PROCESS FOR PRODUCING MODIFIED INORGANIC POLYMERS, THEIR USE IN PRODUCING POLYNUCLEOTIDES, AND A REAGENT USEFUL IN THESE PROCESSES

Abstract: Modified inorganic polymers represented by the formula wherein P ○ is an inorganic polymer linked to the 3′ or 5′ -0- of the nucleoside through a base hydrolyzable covalent bond; R is H or a blocking group; R₁ is a hydrocarbyl radical containing up to 10 carbons; each B is a nucleoside or deoxynucleoside base; and each A is H or OR. Said inorganic polymers are chemically bound to a nucleoside. The chemical bonding of the nucleoside moiety to the inorganic polymer is by means of reactive groups on the polymer which react with reactive groups of the nucleoside molecule. Such modified inorganic polymer supports are useful in the stepwise addition of nuc­ leosides or oligonucleotides to the original nucleoside moiety of the support by a series of process steps. The starting nuc­ leoside which is blocked in either the 3′- or the 5′-OH and the nucleoside is coupled through the unblocked hydroxy group to the polymer support by the coupling agent. Furthermore an automated apparatus for producing a modified silica gel which is an example of a modified inorganic polymer is shown.

Description: Modified inorganic polymers, process for making same and using them in methods for producing polynucleotides and an apparatus to be used in said process The present invention relates to modified inorganic polymers and to methods of making such modified inorganic polymers. Additionally, it relates to methods of producing polynucleotides utilizing said modified inorganic polymers as a support structure. Numerous attempts have been made to develop a successful methodology for synthesizing sequence defined oligonucleotides. However, the stepwise synthesis of polynucleotides, and specifically oligonucleotides still remains a difficult and time consuming task, often with low yields. One prior art technique has included the use of organic polymers as supports during polynucleotide synthesis. Classically the major problems with'polymer supported synthesis strategies has been inherent in the nature of the polymer support. Various prior art polymers used in such syntheses have proven inadequate for reasons such as: (1) slow diffusion rates of activated nucleotides into the support; (2) excessive swelling of various macroporous, low crosslinked support polymers; and (3) irreversible absorption of reagents onto the polymer. See for example, V. Amarnath and A.D. Broom, Chemical Reviews 77, 183-217 (1977). Modified inorganic polymers are known in the prior rt, primarily for use as absorption materials, for example, Ln liquid chromatography. The attachment of nucleosidephosphates to silica gel using a trityl linking group is described in the prior art (H. Xoster, Tetrahedron Letters, 1527-1530, 1972) out the method is apparently applicable only to pyrimidine zucleosides. The cleavage of the nucleoside from the silica support can only be accomplished with acid to which the purine zucleosides are sensitive. The production of phosphotriester derivatives of Dligothymidylates is described in the literature (R.L. Letsinger snd W.B. Lunsford, Journal of the American Chemical Society, 98:12, 3655-3661) by reaction of a phosphorodichloridite with a 5'-O blocked thymidine and subsequent reaction of the product with a 3'-o- blocked thymidine followed by oxidation of the resulting phosphite to a phosphate and removal of blocking groups to obtain the.phosphotriesters;using this procedure, the tetramer and pentamer products, dTpTpTpT and dTpTpTpTpT in which T is thymidine were prepared. Unfortunately, the process requires separation and purification of products at each stage to ensure. proper sequencing of the added nucleosides. Separation techniques including precipitation and washing of precipitates are necessary to implement each successive stage reaction. The present invention provides new and useful modified inorganic polymers. It also provides a new process for producing suc inorganic polymers. In general, the modified inorganic polymer supports of the present invention comprise the inor gan:c polymer to which is chemically bound a nucleoside. The. chemical bonding of the nucleoside moiety to the polymer is by means of reactive groups on the polymer which react with reactive groups of the nucleoside molecule. Representative combinations of such groups are amino with carboxy to form amide linkages between the nucleoside and the support, or hydroxy with carboxy to form ester linkages between the respective moieties. To accomplish the requisite chemical bonding, each of ¯the reactants must of course contain the necessary reactive groups. Thus, the polymer support can be provided with terminal carboxy functionality which will react with hy droxyl and/or amino groups of the nuclosid, Alternatively, the nucleoside can be provided with carboxy functionality by acylation of the'hydroxyl and/or amino groups using a dicarboxylic acid and the carboxy-functional nuoleoside reacted with hydroxy or amino groups of the polymer support. Hydroxy ad amino functionality where not present on the inorganic support can be introduced by known methods. For example, with silica supports, amino functionality can be introduced by reaction with aminoalkylsilyl halides. Of course, the nucleoside moiety of the present modified inorganic polymers can include more than one nucleoside and may include a number of nucleosides condensed as olngonucleotides with the oligonucleotide being attached to the inorganic polymer support through the single chemical linkage, e.g. ester linkage. The thus modified inorganic polymer supports are useful in the stepwise addition of nucleosides or oligonucleotides to the original nucleoside moiety of the support by:a series of process steps as described hereinafter. Subsequently, the polynucleotides so produced are released from the polymer support and recovered from the polymer by a series of process steps including alkali hydrolysis of the chemical bond between the polynucleotide.and the The present invention is particularly useful in the chemical synthesis of any deoxyribonucleic acids (DNA) and ribonucleic acids .(RNA) which contain any deoxynucleotides, nucleotide, polynucleotide, and polydeoxynucleotide, as well as polypeptides. Thus natural DNA and RNA as well as new DNA and RNA can be syntheisized, A wide variety of inorganic polymers can be employed in the present invention and these include, for example, silica, porous glass, aluminosilicates, borosilicates, metal oxides such as alumina and nickel oxide, and various clays. The polymer should be substantiall insoluble in the reaction solvents employed and relatively chemically inert to the reagents employed during processing, except of course for the chemical reactivity required to form the hereinbeforedescribed chemical bond with the initial nucleoside through which the eventual polynucleoside is attached to the support. The process of the present invention is accomplished by treatment of the nucleotide or nucleoside-modified inorganic polymer support by a series of sequential steps whereby each series results in the addition of nucleotide to the modified support until the desired sequence of nucleotides is obtained. The series of sequential steps is as follows: (a) coupling of a selected nucleoside through a phosphite linkage to the nucleoside bound to the polymer support, i.e. the nucleoside-modified support; (b) optionally, but preferably blocking unreacted hydroxyl groups on the nucleotide of the polymer support; (c) oxidation of the phosphite linkage of step (a) to form a phosphate linkage. (d) removal of protecting group from the selected nucleoside described in step (a) to regenerate a reactive site for the next cycle of these steps. Each nucleoside is added sequentially to the polymer support by repetition of steps a, b, c and d until the final oligonucleotide is obtained, after which the oligonucleotide is then removed from the support by hydrolysis reaction which can also remove blocking groups from the oligonucleotide molecule. The removal of blocking groups and hydrolytic cleavage of the oligonucleotide from the support can be accomplished stepwise, which is preferred, or in a single hydrolysis reaction. The nucleoside-modified- support is prepared by covalently coupling a nucleoside to the inorganic polymer using a suitable coupling agent through the 3'for the 5' OH of the starting nucleoside. This is accomplished with the starting nucleoside being blocked in either the 3'- or the 5-OHS and the nucleoside is coupled through the unblocked hydroxy group to the polymer support by the coupling agent. After condensation, residual reactive groups, e.g. carboxy groups, which did not react can be blocked by suitable means, e.g. conversion of carboxy groups to carboxamide by reaction with simple amines. Thereafter, the blocked 3'- or 5'-hydroxy group is converted to free hydroxy by removal of the blocking group and the free hydroxy group is available for condensation with a selected nucleoside containing a phosphite linking groups as in step Ca) hereinbefore described. A variety of coupling agents or groups on the polymer support can be used to covalently link the initial nucleoside or oligonucleotide to the polymer support Representative groups include amino, especially primary amino, hydroxy, thiol, sulfonic acid, phosphorous and phosphoric acid, particularly in the form of acid halides, especially chloro and bromo and carboxy, among others. These reactive groups are conveniently attached to the polymer support commonly through a hydrocarbyl radical such as an alkylene or phenylene divalent radical, one valence position being occupied by the chain bonding and the remaining attached to the reactive groups. Such hydrocarbyl groups may contain up to about 10 carbon atoms, preferably up to about 6 carbon atoms. Alkylene radicals are usually preferred containing 2-4 carbon atoms in tht principal chain. The nature of the reactive group which bonds the nucleoside to the polymer support is not critical but should preferably be readily hydrolyzable to permit separation of the oligonucleotide product from the polymer support at the conclusior. of the preparative process. If desired, the aforesaid coupling groups can be present on the nucleoside for reaction with reactive groups, e.g. hydroxy or amino, on the support polymer. Normally it is preferred to have the coupling groups on the polymer support. The process of this invention, and the new and useful nucleoside-modified inorganic polymer support used therein are particularly advantageous in that they grovide a rapid synthetic route to oligonucleotides and oligodeoxynucleotides which is characterized by high yields and high purity. Each mononucleotide addition requires maximally 2-3 hours with yields of 95% and greater being obtained for each addition. Further, these same yields are obtained as the oligonucleotide grows in size. While the invention can be implemented with a variety of inorganic polymers, it will be described herein in more detail utilizing silica gel as the polymer support. A particularly preferred silica gel is macroporous silica which is used in high performance liquid chromatography (hplc). In addition, the invention will be described using deoxynucleotides but it should be understood that ribonucleotides can t y substituted therefor to obtain similar results. As employed herein, the terms nucleoside, nucleotide and oligonucleotide are intended to include the deoxy counterparts which differ only in the absence of a hydroxy group in the 2' position. Thus, these terms include structures wherein the 2t position substituent is H or OH (as shown hereinafter by substituent A in formulae I, I-I and III). (A) Preparation of nucleoside-modified support. The silica gel support is preferably linked to the silica gel support through a linkage which is readily hydrolyzable, preferably with a weak base such as ammonium hydroxide. The most preferred linkage is an ester linkage which readily hydrolyzes in a weak base such as ammonium hydroxide. This linkage can be accomplished by first linking carboxy functionality to the support or by preforming the ester linkage on the nucleoside by esterification followed by condensation of the esterified nucleoside through the esterifying acid moiety to the support. The first of these embodiments can be accomplished by the following steps: (1) conversion of silica gel to a matrix containing aminoalkyl groups or hydroxyalkyl groups covalently bound thereto; (2) reaction of the aminoalkyl silica with a dicarboxylic acid to form an amide or ester linkage and carboxy functionality; (3) blocking unreacted silanol OH groups; (4) condensation of the free carboxy groups of the silica with the free hydroxy (3'- or.5'-) of the selected nucleoside; and (5) blocking unreacted carboxy groups by conver sion to unreactive derivatives, e.g. amides. The alternative embodiment involves the following steps: (1) conversion of silica gel to matrix containing aminoalkyl grouPs or hydroxyalkyl groups; t21 block unreacted silanol OH groups; C3) join the derivatized silica gel through amide or ester formation to the free carhoxy group of a selected nucleoside which has been modified to contain the half ester of a dicarboxylic acid; and (4) blocking unreactive amino or hydroxy groups on the silica gel support, e.g. using acetic anhydride. Both embodiments give the same product from identical reactants. The second embodiment however is preferred since it leads to more control of the amount of nucleoside loaded onto the silica gel. Additionally, the second embodiment leads to more nucleoside joined to the silica gel (approx imately 100-120oL mole/g compared to 10-40 *, mole/g by the first embodiment). Preferably, the nucleoside is linked to the silica gel through the 3'-OH group rather than the 5'-OH leaving the 5'-OH available for linkage through phosphite to the added nucleoside. Thus, linkage of the added nucleoside occurs at the 3'-OH group and the 5'-OH remains available for linkage to a further added nucleoside. Accordingly, to accomplish the desired linkages at the 3'-OH and 5'-OH respectively, the initial nucleoside is linked through the 3'-OH to the silica gel by the coupling reaction previously defined herein. This is accomplished by blocking the 5'-OH e.g. by use of trityl groups, such as the dimethoxytrityl group,which are preferred since they are readily removed after the initial 3'-OH coupling reaction occurs. When the initial nucleoside includes amino groups, e.g. guanosine, adenosine, cytidine, deoxyguanosine, deoxyadenosine and deotcvtidine, it is preferred to block these groups using known acylating techniques, e.g. with acetic acid, benzoic acid, isobutyric acid and like acids and such blocking group can be removed when convenient, usually after the final oligonucleotide is obtained. The aminoalkyl groups are incorporated on the silica gel by reaction of aminoalkyl-trialkoxysilane which is conveniently accomplished by refluxing in a solvent, e.g. toluene, for several hours. Suitable reagents include aminopropyltriethoxysilane, 4-aminobutyltrimethoxysilane, 4-aminobutyltriethoxysilane, 2-aminoethyltriethoxysilane and others. The dicarboxylic acid employed in forming the ester linkage of deoxynucleoside to the silica gel can be any of a variety such as succinic, glutaric, adipic, phthalic, maleic and similar such dicarboxylic acids of the aliphatic or aromatic type containing preferably up to about 10 carbon atoms. Esterification with the dicarboxylic acid is best accomplished by using the acid anhydride to assure monoesterification. The product produced, i.e. the nucleoside- modified silica gel, can be represented by the following formula: EMI9.1 wherein 3 is the nucleoside or deoxynucleoside base; Q represents the silica support and the covalent linking group which is preferably represented by the formula Csilica gel) (CH2)ntlHCO Z COin which n is an integer from 1-5 and Z is divalent hydrocarbyl radical including alkyl, alkenyl, cycloalkyl. aryl and aralkyl of up to about 10 carbon atoms; A is H or OR; and R is H or a blocking group, e.g. trityl, methoxytrityl, dimethoxytrityl, dialkylphosphite, pivalylisobutyloxycarbonyl, t-butyl- dimethylsilyl, and similar such blocking groups, Formation of phosphitic-linked oligonucleosides The deoxynucleoside-modified silica gel is condensed with a selected nucleoside through formation of a triester phosphite linkage between the 5'-OH of the deoxynucleoside of the silica gel and the 3'-OH of the selected deoxynucleoside. The phosphite linkage can be produced by first incorporating; the phosphite group onto the 5'-OH of the nucleoside on the silica gel followed by condensation with the added nucleoside through the 3'-OH. Alternatively, and preferably, the phosphite. group is incorporated into the added nucleoside at the 3'-OH (the 5'-OH being blocked as by tritylating) and the resulting nucleoside phosphite then reacted with the 5'-OH of the nucleoside on the silica gel. The deoxynucleoside-modified silica gel can also be condensed with a selected nucleoside through formation of a triester phosphite linkage between the 3'-OH of the deoxynucleoside of the silica gel and the 5'-OH of the selected deoxynucleoside. The phosphite linkage can be produced by first incorporating the phosphite group onto the 3'-OH of the nucleoside on the silica gel followed by condensation with the added nucleoside through the 5'-OH. Alternatively and preferably by this approach, the phosphite group is incorporated into the added nucleoside at the 5'-OH(the 3'-OH being blocked as by tritylating using art form procedures) and the resulting nucleoside phosphite then reacted with the 3'-OH of the nucleoside on the silica gel. The general reaction can be represented by the following: EMI11.1 The preferred reaction is represented as follows: EMI12.1 wherein A, B and are as previously defined, R is a blocking group as previously defined, R1 is lower alkyl and X is halogen, preferably C1 or Br, or a secondary amino group attached through the amino nitrogen. The secondary amino group represented by substituant X is preferably one which is formed by removal of the hydrogen atom from a ring nitrogen of a nitrogen heterocyclic compound which contains unsaturated bonds in the ring structure. Exemplary nitrogen-containing heterocyclics include tetrazole, substituted imidazoles such as nitroimidazole, indole pyrazoie, imidazole, benzimidazole, isoindole, pyrrole, triazole, dioxazole and similar heterocyclics, as well as analogs and homologs thereof. When X is such a secondary group, the resulting product is very reactive andsomewhat unstable at ordinary temperatures. In present experience, these compounds should be freshly prepared as needed, or alternatively they can be prepared, isolated and stored in sealed containers at reduced temperature, usually well below 0 C. and usually at about -200C. The removal of the blocking group R permits reaction with a further nucleoside of formula II and repeat reaction gives rise to the polynucleotide of determined sequence of nucleotides attached to the silica gel through the covalently-bonded linking group, e.g. ester linking group. The phosphite linking group is introduced into the nucleoside moiety of the silica gel at the 5'-OH position or the 3'-OH position of the added nucleoside by reaction with a hydrocarbyl (as previously defined herein) phosphorodichloridite, e.g. methyl phosphorodichloridite, preferably in the presence of a base, such as an organic amine. The resulting compound of formula II can be stored in solvent for about one week under an inert gas such as nitrogen or argon and temperatures below about -200C. The reaction of compounds of formula I with those of formula II > is effected in the presence of a base, such as an organic amine, preferably tertiary organic amines, e.g. pyridine, lutidines and similar amines. Blocking Reactions After condensation of the selected nucleoside through phosphite linkage to the nuceloside or oligonucleotide attached to the silica gel support, a small but significant amount (about 1-5%) of the nucleoside or oligonucleotide attached to the silica gel does not react with the added nucleoside. These unreactive moieties preferably are capped or blocked in order to prevent the formation of several deoxyoligonucleotides with heterogeneous sequences. This capping or blocking step can be accomplished by reaction with a very reactive phosphite to form a 5'-phosphite ester group, a relatively nonhydrophobictriester. For example, diethoxytriazolylphosphine can be used to form the diethyl phosphite-5'-deoxynucleoside triester. Corresponding di-lower alkoxy nitrogen-containing heterocyclylphosphines can be used in lieu of the triazdlylphosphine, e.g. tetrazolyl, imidazolyl and 4-nitroimidazolyl phosphine, to produce the corresponding di-lower alkyl triester. These nitrogen-heterocyclyl -phosphines are prepared from the corresponding phosphinyl chloride) of course, the phosphinyl chloride can be used to phosphinylate the nucleoside but the nitrogen heterocyclyl phosphines are preferred since their use leads to higher yields. 150re traditional blocking or capping groups can be employed such as acid anhydrides like acetic anhydride and arylisocyanates like phenyl isocyanate but these react more slowly with the unblocked 5'-hydroxy group. When acetylation with acid anhydrides, e.g. acetic anhydride, is conducted in the presence of tertiary amines, especially di-loweralkylaminopyridines like dimethylaminopyridine, acylation occurs rapidly and this procedure is preferred for blocking especially the 59-hydroxy group. The dialkylphosphite capping group can also be used. The resulting triester is relatively nonhydrophobic and a preferred purification involves reverse phase high performance liquid chromatography which assures separation of the nonhydrophobic by-product from the product containing the hydrophobic 5'-O-dimethoxy- trityl group. To block unreacted silanol hydroxy groups on the silica gel befora nucleoside addition, the use of trialkoxysilyl chloride is preferred, although blocking can also be accomplished by acylation with hydrocarbylmonocarboxylic acids, preferably containing up to 10 carbon atoms, such as acetic, benzoic, butyric, isobutyric and naphthoic acids. Oxidation of phosphite to phosphate The oxidation is normally carried out using iodine as oxidizing agent using standard procedures. Alternatively the oxidation can also be accomplished by reaction with peroxides like tertiary butyl peroxide and benzoyl peroxide as well as hydroperoxides. The use of hydrogen peroxide can lead to the formation of side products and is not preferred. Oxidation should be effected before further condensation of nucleoside is attempted to obtain best yields. Attempts to defer oxidation until after all condensation reactions are completed have resulted in reduced yield of oligonucleotides due to formation of side products. The removal of blocking groups is accomplished by art recognized procedures using mild bases such as ammonium hydroxide whether at room temperature or at elevated temperature1 In stepwise removal of blocking groups, it is preferred to first remove the alkyl group, e.g. methyl, from the phosphotriesters using triethylammmonium thiophenoxide in solvent, e.g. dioxane or tetrahydrofuran. Thereafter, the product is treated with ammonium hydroxide at room temperature (200C.) to hydrolyze the ester linkage joining the oligonucleotide to the support. Then N-acylblocking groups, e.g. acetyl, benzoyl, and isobutyrl, are removed by warming at 500C. for about 12 hours. The removal of trityl blocking groups is conveniently effected employing Lewis acids, particularly zinc bromide, although other Lewis acids have also been effective, e.g. A1C131 3F3 and TiC14. Usually nitromethane is used as solvent for this reaction although other solvents such as tetrahydrofuran can be used, as well as mixed solvents such as nitromethane and a lower alkanol, such as methanol. Alternatively, erotic acids such as toluene-sulfonic acid can be used to remove the blocking group. With purine nucleoside-containing products, however, some depurination can occur when protic acids are employed and therefore the use of Lewis acids is preferred for removal of the blocking group from purine containing products. Employing the hereindescribed process, oligonucleotides containing up to 10-30 nucleoside units can be produced. The oligonucleotides can be converted by T4-ligase and T- 4 kinase to form a DNA sequence of choice by knocm enzymological reactions. The products as obtained after hydrolysis can be purified by standard procedures after separation from the inorganic polymer support. The firal purification is preferably by reverse phase hplc of the 5'-O-dimethoxytrityloligonucleotide as previously mentioned herein, followed by removal of the dimethoxytrityl group, e.g. using a lower alkanoic acid such as acetic acid. The accompanying drawing is a schematic flow dia grarr. of the apparatus of the- present invention. More specifically, and with reference now to the drawing, an apparatus is illustrated to accomplish the foregoing. The column 10 is packed appropriately with solid silica gel matrix 12, derivatized as described herein. Valve 14 is appropriately programmed under control of valve controller 15 to select among the four active reagents contained in reservoirs 16, 18, 20, and 22, and the wash solvents contained in reservoirs 24 and 26. Valve 14 will permit the independent selection from any reservoir in any order without the need to sequence through intervening reservoirs. Thus, for example, the reagent from reservoir 16 may be selected, and immediately thereafter the wash solvent from reservoir 24. These reagents are required for chain elongation in accordance with the teaching of the method of this invention and are maintained at room temperature for use therein. Valve 28, is appropriately programmed under control of controller 15' to select among the five nucleoside-active phosphite triester contained in reservoirs 30, 32 34, 36 and 38, and the wash solvent in reservcir 40. Once again, valve 28 permits independent selection (to prevent cross contamination) as described above, In addition, ze reservoirs 30-38 are designed to maintain the adducts at -780C. and the valve 28 to allow for the passage therethrough at this temperature. Valve 42 is under the control of programmed controller 15" for the selection of cleavage reagents contained in reservoirs 44 and 46 and a wash solvent in reservoir 48. These reagents and solvent are necessary to cleave the oligonucleotide from the support matrix 12 in column 10 and are maintained at room temperature. The valve 50 operates in conjunction with pump 56 to selectively convey solvents, reagents or adduces from the valve 14, 28 and 42 towards the valve 52. In turn, this valve 52, under suitable control of a valve controller (not illustrated) selects between flow through the column 10 and uv detector 58 or recycling through column 10. Valve 54 is controlled to direct the flow front uv detector either to waste or collection. The uv detector 58 is utilized in the control of the system, through suitable feedback control means (not illustrated). The programmed operation of the system is illustrated schematically in Table I below. This program illustrates the protocol for the addition of one nucleoside and then cleavage of the chain from support 12. It will be apparent that the system may be expanded and/or modified to accomodate the addition of more than one nucleotide, and that the entire system will preferably operate under control of a suitably programmed microprocessor computer. The apparatus has particular applicability for automated operation, details of which are within the preview of one skilled in the art. TABLE I Valve 14, 28, 42/ Valve 50 Valve 52 Valve 54 Pump Step Duration Selection Anode Selection Anode Selection Anode Selection Anode Speed 1 2 min. V-14/16 V-14 Flush Waste L 2 60 min. V-14/16 V-14 Recycle " L 3 5 min. V-14/24 V-14 F " H 4 2 min. V-14/26 V-14 F " H 5 2 min. V-14/18 V-14 F " L 6 2 min. V-14/18 V-14 R " L 7 5 min. V-14/26 V-14 F " H 8 2 min. V-14/20 V-14 F " L 9 2 min. V-14/20 V-14 R " L 10 2 min. V-14/24 V-14 F " H 11 3 min. V-28/40 V-28 F " H 12 20 sec. V-28/30 V-28 F " L 13 5 sec. V-28/30 V-28 F " L 14 60 min. V-28/30 V-28 F " L 15 10 sec. V-28/30 V-28 R " L 16 30 sec. V-28/30 V-28 F Collect L 17 Stop 18 2 min. V-14/22 V-14 F Waste L 19 30 min. V-14/22 V-14 R " L 20 5 min. V-14/26 V-14 F " H 21 5 min. V-14/24 V-14 F " H One Nucleodite Added; Cleavage Sequence: 1 2 min. V-42/44 V42 F Waste L 2 60 min. V-42/44 V42 R " L 3 5 min. V-42/48 V42 F " H 4 30 sec. V-42/46 V42 F " H 5 180 min. V-42/46 V42 R " L 6 10 min. V-42/46 V42 F Collect L 7 10 min. V-42/48 V42 F " L The silica gel which is used as starting material in the production of the modified silica gels of the present invention is not critical. Silica gel particles in the range of from about 5 m to about 1000 m are useful, with particles in the range of about 10 m to about 50 m being preferred. In a similar manner pore size is not critical. Pore sizes in the range of about 50 to about 2000 are preferred. The modified silica gels of the present invention: (1) allow relatively rapid diffusion of activated nucleotides, and other reagents into the support; (2) avoid swelling; and (3) resist adsorption of reagents. Additionally, the modified silica gels of the present invention are (1) insoluble in most solvents; (2) in use as support matrices, allow solvents and unwanted reaction products to be easily washed from the matrix, while maintaining the desired reaction products in place and capable of continuous processing; and (3) allow the supported material to react relatively rapidly and in high yield, for example, in cylindrical condensation. The modified silica gel employed to react with the initial nucleoside of the oligonucleotide chain to form the initial reactive material is prepared by art-recognized procedures. The production of a variety of functional groups on the surface of the silica gel suitable for reaction with the hydroxy (3'- or 5'-) of the initial nucleoside can be effected using known methods, e.g. methods described in U.S. Patents 3,519,538; 3,419,517; 3,652,761; and 3,669,841. The preferred method for the present invention is to introduce amino functionality on the silica by reaction with an aminoalkyl silane derivative, e g. by reaction of a trialkoxy 3-aminopropylsilane such as triethoxy 3-amino-propyl- silane with the silica support to form the covalent linkage: EMI20.1 and the amino group is reacted with one carboxy group of a dicarboxylic acid therefor giving rise to carboxy functionality on the silica gel where condensation of amino and carboxy occur. The silica gel can next be treated to block unreacted silanol groups with suitable blocking agents, e.g. trialkylhalosilanes such as trimethylchlorosilane or the bromo analog. The resulting carboxy-derivatized silica can then be reacted with the hydroxy group (3'- or 5'-) of the first added nucleoside. Alternatively; as previously indicated herein, the dicarboxylic acid can be reacted with the selected nucleoside to form a monoester at the 3'-O or 5'-o and the resulting ester containing a free carboxy group in the esterifying radical can be condensed with the amino-derivatized silica to form the same covalent linkage between nucleoside and silica support. Any unreacted amino groups of the aminoderivatized silica gel are preferably blocked by acylation with monocarboxylic acids such as acetic, benzoic or isobutyric acids, normally employing the acid anhydrides under acylating conditions. The structure of the covalent linkage between the first nucleoside and the silica support is not critical as long as a substantially stable covalent linkage is formed to bind the nucleoside during the sequential nucleoside addition cycles. The covalent linkage therefore should be stable to the sequential reaction conditions but should be reasonably readily hydrolyzable to permit recovery of the formed oligonucleotide after completion of nucleoside addition. Thus, ester and amide linkages are particularly effective with the desired degree of stability and, at the same time, being readily hydrolyzable after nucleoside addition is completed using weak or strong bases. As used herein the symbols for nucleotides and polynucleotides are according to the IUPAC-IUB Commission of Biochemical Nomenclature Recommendations [t1970) Biochemistry 9, 40223. The following examples further illustrate the invention. EXAMPLE 1 A. Polymer supports functionlized with carboxylic acid groups are prepared from silica gel. A separation group silica gel supplied by Vydak as TP silica, having 20 jim particle size, 300 pore size is used as the starting material. The silica gel was placed in a desicator over a saturated LiCl solution for 24 hours. The initial step in the process is silylation by refluxing 3-aminopropyltriethoxysilane , (2.3 g, 0.01 M) with the silica gel (2.6 g) in dry toluene. After silylation is substantially complete, in this case after about twelve hours of refluxing, the reaction mixture is cooled and the toluene solution removed. The thus silylated silica gel is then washed serially with toluene, ethanol and then with ether and air dried. Succinic anhydride (2.5 g, 0.025 M) in water is next reacted with the silane modified silica gel to provide carboxylic acid functionality to the terminal portion of the covalently bonded silane side chains. During this latter reaction, the pH is maintained between 4 and 6 by addition of a base, such as 2 N sodium hydroxide. After this latter reaction, which proceeded for about 6 hours, the modified silica gel containing carboxylic acid functional groups on its side chains is washed with water, then with methanol and ether, and then finally dried in vacuum at room temperature. The modified silica gel is than treated with trimethylsilylchloride [(CH3)3 SiCl, 1.09 g, 0.01 M] in anhydrous pyridine by refluxing for about 12 hours. The resulting modified silica gel is then washed with 5% trichloroacetic acid in water, then with water, and then with ethanol and ether. After drying in vacuum, the yield of carboxylic acid functionality on the modified silica gel is about 250 mole/g. B. 5'-o-dimethoxytrityldeoxythymidine (1.17 g, 0.002 M) and the modified silica gel described in A (4 g, 0.001 mole carboxylic acid functional group) are reacted for about 40 hours in anhydrous pyridine using dicyclohexycarbodiimide (2.06 g, 0.01 M) as condensing agent. The unreacted residual carboxylic acid groups in the modified silica are blocked, by the addition of p-nitrophenol (1.4 g, 0.01'M) followed by the addition of 10% piperidine in pyridine (25 minutes). The reaction product is then washed serially with tetrahydrofuran, methanol and finally with ethyl ether. Then, as a precaution to assure complete blockage of unreacted carboxylic acid, the composition is first treated with dicyclohexylcarbodiimide and p-nitrophenol and then piperidine in pyridine for a second time. After removal of the dimethoxytrityl group using 0.1 N p-toluenesulfonic acid in acetonitrile, the yield of thymidine attached to the support is found by spectrophotometry to be about 40 mole/g. EXAMPLE 2 A. Silica gel (Vydac A, 25 gms) was placed in a desiccator over a saturated LiCl solution for 24 hr. The silica gel was transferred to a 500 ml round bottom flask, toluene (250 ml) and aminopropyltriethoxysilane (13 ml) were added, the flask was tightly sealed, and the suspension was gently shaken for 12 h at room temperature. The flask containing the suspended silica gel was next refluxed for 18 h. Before starting the reflux, add one boiling chip to the solution. Following the reflux step, the silica gel suspension was transferred to a centrifuge bottle and the silica gel pelleted by a low speed'spin. The supernatant was decanted and the silica gel was washed with toluene (3 X, 80 ml ea), methanol (3 X, 80 ml ea) and methanol:H20, 1:1 (2 X, 80 ml ea). The silica gel was next suspended in 80 ml 50% aqueous methanol and shaken overnight at room temperature. Once again the silica gel suspension was isolated by transfer to a centrifuge bottle followed by a low speed spin. The silica gel was next washed with methanol C2 X, 80 ml ea) and ethyl ether (3 X, 80 ml ea). Finally, the silica gel was air dried for 6 h and then dried in vacuo. The silica gel was placed in a round bottom flask. A solution of dry pyridine (50 ml) and trimethylsilyl chloride was added and the suspension shaken at room temperature overnight. The silica was isolated by low speed centrifugation. The silica was then washed with methanol (5 X, 80 ml) and ethyl ether (3 X, 80 ml). The silica gel was air dried for 6 h and then dried in vacuo. B. The 5'-O-dimethoxytrityl and N-protected deoxynucleoside (2.5 mole) was dissolved in a solution of dry pyridine (5 ml) and N, N-dimethylaminopyridine (0.3 g). Succinic anhydride (2.0 mmole, 0.2 g) was added and the solution stirred at room temperature for 12 h. Thin layer chromatography (tlc) in acetonitrile:water (9:1, v/v) can be used to monitor the reaction. Unreacted nuceloside will have an Rf of approximately 0.8 whereas the product will be a smear from Rf 0.3 to Rf 0.5. After completion of the reaction, solvent is removed in a rotary evaporator and the dry gum is redissolved in toluene (10 ml). Toluene is removed using a rotary evaporator and the toluene co-evaporation procedure is repeated. The dry gum free of pyridine and N, N-dimethylaminopyridine is dissolved in methylenechloride (30 ml). This solution is transferred to an extraction funnel and 10% ice-cold citric acid is added. After vigorous shaking and extraction, the organic phase is washed twice with water (15 ml ea) and then dried over sodium sulfate. Approximately 0.3 ml pyridine is added to the methylene chloride solution in order to minimize detritylation while drying over sodium sulfate. The methylene chloride solution is concentrated to 10 ml and the succinylated nucleoside isolated by precipitation into hexane:ether (1:1, v/v; 250 ml). The precipitate is collected by centrifugation and dried in vacuo. To obtain the nitrophenyl esters, succinylated nucleoside (1 mmole) was dissolved in dry dioxane (3 ml) containing pyridine (0.3 ml). DCC (10 mmole, 0.22 g) and p -nitrophenol (0.14 g, 1 mmole) were added and the solution shaken for 2 h. Dicyclohexyl urea was removed by centrifugation. Analysis by tlc in acetoitrile:,O (9: v/v) indicates the product with an Rf of 0.8. This supernatant free ofdicyclohexylurea is used directly for coupling to silica gel. Silica gel prepared as outlined in A of this example, (5 g if 50 mole nucleoside/g desired; 2.5 g if 100 mole nucleoside/g desired) was suspended in dry DMF. The p-nitrophenylsuccinylated nucleoside derivative (supernatant prepared herein) was added to the silica gel and the resulting suspension was shaken for two hours. An aliquot of silica gel (approx. 1 mg) was then removed for analysis. After washing > the aliquot with DMF(2 X), methanol (3 X) and ethyl ether (2 X), 0.1 M toluenesulfonic acid in acetonitrile (1 ml) was added to the aliquot and the trityl released from silica as a red-orange color was observed. This analysis ctn be completed quantitatively if desired. If this analysis appears satisfactory (i.e. a positive trityl test), the bulk of the silica gel was washed with DMF (.3 X, 10 ml ea), dioxane (3 X, 10 ml ea), methanol (5 X, 10 ml ea), and ethyl ether (3 X,-10 ml ea). Unreacted n-propylamino silyl groups were then blocked with a solution of acetic anhydride (0.7 ml) and dry pyridine (5 ml). The silica gel was isolated by centrifugation, decanting and repeated washing with methanol (4 X, 10 ml ea) and ethyl ether (2 S, 10 ml ea). The assay for completeness of the capping or blocking of n-propylamino groups is as follows. Take an aliquot (1 mg) of: (1) Underivatized Vydac-A, (2) Vydac derivatized with the aminopropyltriethoxysilane, (3) Vydac that has had nucleoside attached and subsequently blocked with acetic anhydride. Each sample was then treated with 2501 of saturated sodium borate containing 0.2 m/ml picryl sulfate. Vortex and centrifuge the reactant products. The underivatized Vydac should remain white. The aminopropylsilyl Vydac should appear bright Orange-red. The capped Vydac will be pale yellow-orange. This is probably due to interaction of picryl sulfate with rine nitrogens on nucleosides. With some preparations, a contaminant of succiny lated n-propylamino groups will result from the presence of succinic acid. This succinic acid may be present because all the succinic anhydride was not cons-imed during the succinylation or alternatively was not removed as succinic acid during the aqueous extraction with citric acid. If succinylated n-propylamino groups are present, they can be blocked in the following manner. The protected silica gel containing succinylated nucleoside (either 5 g or 2.5 g) was suspended in a solution of dry pyridine (5 ml) containing DCC (0.28 g) and p-nitrophenol (0.16 g) and shaken overnight at room temperature. Morpholine (0.2 ml) was then added and the suspension shaken for 10 minutes. Silica gel was isolated after centrifugation, decantation of the supernatant, and washing the silica gel with methanol (4 X, 10 ml ea), THE' (3 X, 10 ml ea) and ethyl ether (3 X, 10 ml ea). After air drying, the silica gel was dried in vacuo. A quantitative assay for the trityl cation and therefore the loading of nucleoside on the silica gel is as follows: 1. Weigh accurately approximately 1 mg of dry silica gel. 2. Add 1 ml of 0.1 M toluenesulfonic acid in acetonitrile. 3. N asure the absorbance at 498 nm. If the absorbance approaches 2.0, dilute and re-reed. The loading can be calculated as follows: (Abs498). (dilution factor) loading in moles/g = wt silica gel in mg X 14.3 If 5 gm silica gel was used, the loading should be approximately 40 mole/g. If 2.5 gm silica gel was used, the loading will be approximately 100 mole/g. EXAMPLE 3 Deoxythymidine phosphomonochloridite is synthesized by the addition of 1.0 equivalent 5'-O-dimethoxytritylthymidine to 0.8 equivalent methylphosphorodichloridite and 5 equivalents collidine in THF at -780C. The resulting compound and a thymine-modified silica gel matrix are utilized in oligonucleotide synthesis. The first step involves packing the thymidine-modified silica gel into a glass column. This column is attached through a series of valves and tubes to a pump and an injector loop. The apparatus is organized so that reagents can be recycled through the column, flushed to waste, or collected. The steps involved in synthesizing thymidylylthymidine attached to the support included: (1) recycling the deoxythymidine phosphomonochloridite derivative in THF and collidine through the column of modified silica gel for about 1 hr; (2) oxidizing the polymer supported dinucleoside phosphite to the phosphate using 0.01 M I2 in water/2, 6 lutidine/THF (30 min); (3) recycling phenylisocyanate in THF and 2, 6 lutidine through the column for 1.5 hr. (this reagent protects against the formation of failure sequences by reacting with unphosphorylated nucleoside hydroxyl groups); t4) flushing the column with toluenesulfonic acid in acetonitrile (2 min). All steps were performed at room temPerature, The total time needed for addition of one nucleotide, including various wash cycles after each step is about 4 hours. This four step procedure was repeated several times in order to produce good yields of two oligodeoxynucleotides, d(T)7 and d(T) attached to-the silica gel matrix. The same procedure as described above is used for preparing d(T-C-T-C-T-C-T-T-T). This cytosine containing phosphomonochloridite is prepared from 5'-O-dimethoxytrityl N-benzoyldeoxycytidine. EXAMPLE 4 Removal of Oligodeoxynucleotides from the Support and Characterization of the Compounds Produced The oligodeoxynuclotides [d(T), d(T)g, d(T-C-T-C-T-C-T-T-T)] are freed from protecting groups, isolated and characterized. The methyl group is removed from phosphotriesters using triethylammonium thiophenoxide in dioxane. This step is followed by treatment with concentrated NH40H which removed the N-benzoyl group from cytosine and frees the oligonucleotides from the support. In each case the major product from each synthesis, as determined by high performance liquid chromatography, is found to be the described heptamer or the respective nonamers. Based on the amount of thymidine initially linked to the support, the isolated yield of d(T)9 is found to be about 25%. The cor responding yield of d(T-C-T-C-T-C-T-T-T) is found to be about 23%. Both nonamers and the heptamer are also biochemically characterized All three compounds are found to be completely degraded by Snake Venom Phosphodiesterase. The oligonucleo-. tides isolated from each nonamer synthesis were phosphorylated using [S1-32PjATP and T4kinase and then analyzed by gel electrophoresis before and after partial degradation with Snake Venom Phosphodiesterase. This analysis confirms that the oligonucleotide is homogeneous and contains nine nucleotide units. In order to confirm the sequence of [5' -32P] d (pT-C-T-C-T-C-T-T-T) the sample is analyzed by two dimension homochromatography. The sequence profile is found to be consistent with the results expected for [5'-32P] d(pT-C-T-C-T-C-T-T-T). Finally, [5'-32P] d (pT) 9 was shown to polymerize in the presence of T4-ligase and polydeoxyadenosine indicating that [5'32P]d(pT)9 forms a duplex with polydeoxyadenosine and that this duplex is recognized by T4-ligase. Therefore, d (T)g and d (T C-T-C-T-C-T-T-T) were biochemically active by every criteria so far tested. In preferred embodiments, the amino groups, such as those on cytosine, adenine and guanine are protected. Protection of these groups is not a necessary part of this process but does enhance nucleoside solubilities in the appropriate solvents. Benzoyl, trityl (as previously defined herein or isobutyryl, groups provide suitable protecting groups, although other protecting groups can be used without altering this process. Protected nucleosides produced with good yields include 5'-O-dimethoxytrityl-deoxythymidine [DMTrd(T)], 5'-O-dimethoxytrityl-Nbenzoyldeoxycytidine tDMTrd (bzC)], 5 '-O-dimethoxytrityl-N- benzoyldeoxyadenosine [DMTrd(bsA)], and 5' -O-dimethoxytrityl N- isobutyryldeoxyguanosine [DMTrd(ibG)] as protected nucleosides. A typical synthesis as. illustrated with deoxyadenosine is as follows. EXAMPLE 5 This example illustrates the use of purine deoxynucleotides. DMTrd(bzA) (.66 g., 1 mmole) in dry THF (3 ml) is added dropwise under an argon atmosphere to a stirred solution of the THF (3 ml) containing methyldichlorophosphite (.113 ml, 1.2 mmole) and 2, 4, 6 trimethylpyridine (.633 ml, 4.8 mmole) at -780C. After 10 minutes at -780C., the reaction solution is filtered through a sintered glass funnel and solvent is removed by concentration in vacuo. Excess methyl phosphodichloridite is removed by dissolving the resulting gum in to,luene: THF (2 ml, 2:1) and reevaporating in vacuo to a gum. This procedure is repeated several times to insure removal of the dichloridite. The nucleoside phosphomonochloridite is converted to the tetrazolide. The gum resulting from the final re-evaporation is dissolved in THF (2 ml). A solution of tetrazole (.063 g, 0.9 mole) in THF (2 ml) is then added dropwise with stirring at -780C. to the nucleoside phosphomonochloridite. After 10 minutes at -780C., the solution is transferred to a centrifuge tube, spun at low speed, and the supernatant is removed. This solution contains the activated nucleoside methylphosphomonotetrazolide. If not used immediately, this tetrazolide can be placed in long term storage after precipitation by dropwise addition into dry pentane, followed by collection, drying in vacuo , and storing in sealed tubes under argon or other inert gas at -200C. All operations are performed under inert gas to avoid oxidation. At no time is the active agent exposed to air. The foregoing procedure is applicable for the preparation of activated thmidine, deoxycytidine, and deoxydenosine nucleotides. For the preparation of the activated deoxyguanosine nucleotide, the procedure is the same except for the stoichiometry. The molar ratio of DMTrd(ibG); methyldichlorophosphite; 2, 4, 6 trimethylpyridine and tetrazole is 1 : 0.9 : 3.8 : 0.7. The steps necessary for addition of one nucleotide to the modified silica gel polymer support follow. The removal of the dimethoxytrityl group from the nucleotide is accomplished by exposing the modified silica gel support to 0.1 M ZnBr2 in nitromethane for 15 to 30 minutes. The support is then washed initially with butanol: 2, 6 lutidine : THF (4 : 1 : 5 by volume) and finally with THF. The solvent ratio is notiimportant since this step is used to remove potential zinc esters of nucleosides. This step could be eliminated but lower yields may result. Other Lewis acids could be substituted for ZnBr2, such as BF3, AlCl3 and TiC14. However ZnBr2 is preferred. Protic acids can also be used. However approximately 3-58 depurination of each purine by protic acids is observed even when the amount of acid is reduced to the minimum amount needed to remove the dimethoxytrityl group. The next step in the process is condensation of the protected and activated nucleotide to the nucleoside or oligonucleotide covalently bound to the support. This is accomplished by using 10-15 equivalents of the activated monotetrazolide and a reaction time of about one hour. The solvent is anhydrous THF. This process may also be used for the addition of the activated monochloridites, triazolides and nitroimidazolides. However, best results were obtained with the tetrazolide. The next step in the process is the blocking of unreacted 5'-hydroxyl groups. This is accomplished using a solution of acetic anhydride, dimethylaminopyridine, pyridine and THF. This may also be accomplished using a 0.33 M solution of diethyl- monotriazolephosphite in 2.6-lutidine/THF (1 : 5 by volume ). The reaction time is 5 minutes and is followed by a THF wash. As a further alternative, a solution of phenylisocyanate/lutidine (45 : 55 by volume) and a 90 minute reaction time may be used for this step. This solution is then removed from the modified silica gel by washing the support with THF and "ith acetonitrile. The first procedure is preferred. This step can be eliminated or other reagents that react with 5'-bydrdxyl groups and are compatible with the overall chemistry can be substituted therefore. However, by including this step, the final purification of the desirable oligonucleotide is rendered much easier. This is because the complexity of the total synthetic material bound to the support is reduced considerably. The final step in each cycle is oxidation of the phosphite to the phosphate. A composition of 0.1 M I 2 in water/2, 6 lutidine/THF (1 :1 : 3) is preferred, although other ratios can be used. Furthermore. other oxidizing agents such as N-chlorosuccinimide or aryl or alkyl peroxides could also be used. T-butyl peroxide is presently preferred as oxidizing agent. After the addition of the appropriate activated nucleotides in any predetermined sequence, the deoxyoligonucleotide is removed from the support as described above in the synthesis of d(T)g. The compounds of formula II herein in which X is a secondary amino group formed by removal of the H atom of the secondary amino nitrogen of a nitrogen heterocyclic compound are new compounds which are particularly useful in forming the requisite phosphorus linkage. These compounds are more reactive and consequently more efficient than the corresponding compounds wherein X is halogen. These compounds are readily prepared from the compounds in which X is halogen (as described, for example, in Example 5) or can be formed by reaction of a halo- (20 amino)-alkoxyphosphine with the selected nucleoside. The use of such heterocyclicaminophosphine compounds is exemplified in the succeeding examples, particularly example 5 which illustrates the preparation of a tetrazolide and use thereof in forming the necessary phosphorus linkage. Employing this procedure, a variety of such compounds are prepared using tetrazole, nitroimidazole and triazole as well as various nucleosides to obtain the corresponding nucleoside phosphonomonamine. Particularly, such compounds include as nucleoside base thymine, cytosine, adenosine and quanine and such compounds are further protected with blocking groups as required, e.g. benzoyl groups on the amino group of cytosine and adenine as well as isobutycyl or the amino group of. quanine. EXULE 6 The following example illustrates the use of purinedeoxynucleotides in the invention. A. HPLC grade silica gel (2 g, Vydac TP-20, Separation Group, 100 m2/g surface area, 300 pore size, 20&num; m particle size) was exposed to a 15% relative humidity atmosphere satd. LiCl) for at least 24 h. The silica (2.0 g) was then treated with 3-triethoxysilylpropylamine (2.3 g, 0.01 M in toluene for 12 h at 200 and 12 h at reflux under a Drierite drying tube. This reaction was completed on 'a shaking apparatus because magnetic stir bars pulverize the silica gel and should be avoided. The silica was isolated by centrifugation, washed successively (twice each) with toluene, methanol and ether and air dried. B. The carboxylic acid group was introduced by agitating the silica so produced (2 g) and succinic anhydride (2.5 g, 0.025 M) in water. The pH was controlled (pH 2-6) by addition of 2 M NaOH. Completeness of the carboxylation reaction was qualitatively monitored using a picrate sulfate test. An aliquot of silica (approximately 2 mg) was treated with 0.5 ml of 0.1 M picrate sulfate in saturated sodium borate buffer (pH 10). The original silica reacted within 10 min and stained a bright yellow whereas the acylated product remained white. The succinic anhydride reaction was allowed to continue until the silica gel remained white during the picrate sulfate test. Usually the total reaction time was one hour and a second addition of succinic anhydride was required. After washing successively (twice each) with water, 0.1 M trichloroacetic acid, water,methanol and ether, compound 2 was air dried, dried in vaco, and then treated with trimethylsilylchloride (1.25 ml, .01 M) in pyridine (7 ml) for 24 h at 250 and the product was then washed with methanol (4 times) and ether. Analysis for extent of carboxylation involved a two step procedure. An accurately weighed aliquot was treated with dicylcohexylcarbodiimide (DCC) and p-nitrophenol in pyridine. After several washings with tetrahydrofuran to remove unreacted P-nitrophenol, 10% piperidine in pyridine was added to the silica gel and the amount of p-nitrophenol released was measured at 410 nm using 1.57 X 104 as the extinction coefficient of p-nitrophenoxide. The incorporation of carboxylic acid was 200)1 mol/g. C. The deoxynucleosides were joined to this product using DCC. 5'-O-dimethoxytritylthymidine (1.1 g, 2.16 mmol) DCC (2 g, 0.01 mol), and 2 (4 g, 0.8 mmol carboxylic acid) were agitated in dry pyridine (21 ml) for 2 days. P -Nitrophenol (1.4 g, 0.01 mol) was added, the mixture was agitated for an additional day, and then the reaction was quenched with morpholine (1 ml, .011 mol). 'After washing with methanol and ether, the silica gel was analyzed for unreacted carboxylic acid. Usually a second treatment with DCC (2 g,0.01 mol) and p-nitrophenol (1.4 g, 0.01 mol) in dry pyridine (20 ml) and finally morpholine (I ml) was necessary to completely block the trace amount of free carboxylic acid ( < 10 r)mol/g) that remains from the first blocking procedure. 5'-O-Dimethoxytritylthymidine, 5'-O-dimethoxytrityl-N-benzoyldeoxycytidine, 5' -O-dimethoxytrityl-N-isobu'ryl deoxyguanosine and 5 '-O-dimethoxytrityl-N-benzoyldeoxyadeno- sine were converted to activated nucleoside by introduction of the requisite phosphinyl chloride group using the following procedure. 5'-O-Dimethoxytritylthymidine (1.6 g, 2.9 mmol) in anhydrous tetrahydrofuran (5 ml) was added dropwise to a well stirred solution at -780 of CH3OPC12 (0.33 ml, 2.5 mmol) and collidine (1.86 ml, 14.1 smol) in anhydrous tetrahydrofuran (5 ml). A white precipitate formed during the addition. The mixture was stirred for 15 min at -780 and then filtered through a sintered glass funnel to remove collidine hydrochloride. The collidine hydrochloride was washed with dry tetrahydrofuran (1 ml). The filtrate was then diluted with dry toluene and concentrated to a gum. After dry argon had been bled into the apparatus, a solution (6 ml) containing toluene:tetrahydrofuran (2:1) was added and the gum was allowed to dissolve completely in this solution. Solvent was removed by concentration in vacuo. This reconcentration using a solution of toluene and tetrahydrofuran was repeated three times. After the final concentration, the gum was dissolved in dry tetrahydrofuran (3 ml), cooled to -780 and a solution of tetrazole (.18 g, 2.6 mmol) in dry tetrahydrofuran (3 ml) was added dropwise. A white precipitate of collidine hydrochloride formed during the addition. The mixture was stirred an additional 10 min at -780 and then transferred using positive argon pressure and a cannula to a centrifuge tube filled with argon. The supernatant recovered after centrifugation contained the tetrazolylphosphite product which can be used directly for synthesis of deoxyoligonucleotides. Alternatively, the tetrazolylphosphite can be stored as a precipitate and reconstituted as needed. The aforesaid phosphites, i.e. activated nucleotides, were used in the synthesis of deoxyoligonucleotides in an automated apparatus in accordance with the accompanying drawing. Synthesis of deoxyoligonucleotides. The apparatus consists of a Milton Roy ninipump,, three way Altex slide valves, a recycle valve (a modified Altex valve) and an injector loop (a three way Altex valve). All connections were with teflon tubing and were designed to minimize the tubing volume in the recycle loop. The column was an 11 mm Ace glass column that had been shortended to approximately 1 ml capacity. Cellulose filters were used to support the silica bed. The filters were acetylated with a solution of acetic anhydride and pyridine (1:1 based on volume) for 4 h at 500 before use. The total volume contained within the recycle loop of this apparatus was approximately 2.5 ml. The tetrahydrofuran reservoir was protected from air with a nitrogen bubbler and the ZnBr2 solution was protected from moisture with the Drierite tube. The various chemical operations that must be performed for the addition of one nucleotide to the silica are listed in Table I. Table I. Protocol for Machine Assisted Polynucleotide Synthesis Reagent or Solventa,b Time (min) Machine Mode Satd. ZnBr2/Ch3NO2 30 Flush CH3(CH2)2CH2OH/2,6-lutidine/THF 5 Flush THF 10 Flush Activated Nucleotide 60 Recycle (CH3CH2O)2P (triazole) 5 Recycle THF 2 Flush I2 Oxidation 5 Flush THF 5 Flush CH3NO2 3 Flush aTHF, Tetrahydrofuran bA nitromethane solution saturated with ZnBr2 is approximately 0.1 M in ZnBr2 Typically, 0.25 g of 3 (10 mole thymidine) was loaded into the column and the silica washed with nitromethane. The 5'-O-dimethoxytrityl group was removed by flushing the column (30 min) with nitromethane saturated with ZnBr2 (approximately 0.1 M in ZnBr2) at a pump speed of 1 ml/min. The completeness of deprotection was monitored visually or spectrophotometrically by observing the release of a bright orange dimethoxytrityl cation. By measuring the absorbance at 498 nm, the completeness of the previous condensation step was monitored. This step was followed successively by a wash with a solution of n-butanol:2,6-lutidine:tetrahydrofuran (4:1:5) for 5 min at a flow rate of 2 ml/min. The next step was a wash for 5 min (5 ml/min) with dry tetrahydrofuran. During the course of this washing step, the recycle valve and the injector port were also flushed with dry tetrahydrofuran and the effectiveness of this wash was monitored at 254 nm using a spectrophotometer. The condensation step was next completed using activated nucleotide that had been reconstituted using dry tetrahydrofuran. The reconstituted solution was stored in a dry ice/acetone bath over argon but condensation reactions were carried out at room temperature. When reconstituted, activated nucleotide stored in this way was stable for several days. Approximately 10 equivalents of activated nucleotide (100 mole for 0.25 g of 4) in 0.5 to 0.8 ml of tetrahydrofuran was injected into the apparatus and the machine switched to the recycle mode. The activated nucleotide was circulated through the silica gel for 1 h at a pump speed of 2 ml/min. Aliquots of activated nucleotide from the apparatus were then collected directly into dry methanol and water. Analysis as described previously indicated whether activated nucleotide was still present in the system. Usually this is the case. However, occasionally (approximately 1 in 10) the bis methyl phosphite of the deoxynucleotide was not observed by this assay. When this occurred, the condensation step was repeated to prevent the possibility of incomplete reaction. The next step ;n- volves capping reacted 5'-O-hydroxyls by adding dieth xytriazoylphosphine (1 ml of a 0.3 M solution in tetrahyd.ofuran) directly to the solution of activated nucleotide and continuing the recycle mode for 5 min at a pump speed of 2 ml/min. Residual activated nucleotide and the capping reagent were then flushed from the apparatus using dry tetrahydrofuran (2 min at 5 ml/min). This step was followed by oxidation of phosphites using a solution of tetrahydrofuran:2,6-lutidine: water (2:1:1) containing 0.2 N 12. The solution was flushed through the apparatus for 5 min (2 ml/min). Finally the cycle was completed by flushing the system first with dry tetrahydrofuran for 3 min (5 ml/min) and nitromethane for 2 min(5 ml/min). This cycle is then repeated an appropriate number of times to complete the desired sequence. Isolation of Deoxyoligonucleotides. The completely deprotected deoxyoligonucleotides were isolated by the following procedure. An aliquot (10 mg) of the silica gel containing the deoxyoligonucleotic. triester in protected form was first treated with thiophenol:triethylamine:dioxane (1:1:2, v/v). After 45 min of gentle shaking, the silica gel was recovered by centrifugation and washed with methanol (4 times) and ethyl ether. Afte: air drying, the deoxyoligonucleotide was removed from the support by a three hour treatment with concentrated ammonium hydroxide at 200 followed by centrifugation. Base protecting groups were removed by warming the supernatant at 500 for 12 h in a sealer tube. The 5'-O-dimethoxytrityldeoxyc ligonucleotide was i slated by concentrating the hydrolysate in vacuo, dissolving the residue in 0.1 M triethylamminium acetate (pH 7.0) and chromatographing this material on a C18 reverse phase hplc column (Waters Associates). The eluting buffer was 0.1 M triethylammonium acetate containing 26% acetonitrile. The peak containing 5'-O-dimethoxytrityldeoxyolgonucleotide was concentrated in vacuo and the residue was treated at 200 for 15 min with acetic acid-water (4:1, v/v) to remove the 5'-O-dimethoxytrityl group. The completely deprotected deoxyoligonucleotide was isolated by concentration of the acetic acid solution in vacuo, dissolving the residue in 25 mM triethylammonium bicarbonate (pH 7), and extraction of dimethoxytritanolwith water saturated ether. Characterization of Deoxyoligonucleotides. The 5'-hydroxyl of each deoxyoligonucleotide was phosphorylated using using t5' -32P]ATP and T4-kinase. The amount of deoxyoligo- nucleotide used in a phosphorylation reaction was determined by measuring the absorbance and using a calculated extinction coefficient which assumed no hypochromicity for the deoxyoligonucleotide. Phosphorylated deoxyoligonucleotides were separated from excess ATP by desalting on a G-50-40 Sephadex column using 10 mM triethylammonium bicarbonate (pH 7) as eluant. Gel electrophoresis on polyacrylamide and two dimension analysis were completed using standard procedures. Synthesis of d(C-G-T-C-A-C-A-A-T-A). Silica gel modified with 5'-O-dimethoxytritylthymidine (0.25 g, 50 m/g) was loaded into the column and the cycle was started by washing the silica gel with nitromethane and removing the 5'dimethoxytrityl group with ZnBr2. Elongation was performed as previously described using an approximate tenfold excess of the incoming activated nucleoside phosphite (.1 inN) at each condensation. Synthesis was continued to the completion of the deoxyoctanucleotide, d(T-C-A-C-A-A-T-T). At this point the silica was divided into two approximately equal portions. One portion was elongated to the deoxydecanucleotide in standard fashion. The overall yield was 64% based on the amount of dimethoxytrityl group bound to the support and 30% was the yield isolated from a reverse phase hplc column. Synthesis of d (A-C-G-C-T-C-A-C-A-A-T-T). The remaining portion of d(T-C-A-C-A-A-T-T) was elongated in standard fashion in the machine to the deoxydodecanucleotide. the overall yield was 55% based on the dimethoxytrityl group bound to the support. The isolated yield was not accurately determined. The following oligonucleotides were prepared using the procedures described: 5 '-d(A-A-T-T-C-A-C-C-G-T-G) 5 '-d(C-G-T-G-T-T-G-A-C-T) 5'-d(A-T-T-T-T-A-C-C-T-C-T) 5'-d(G-G-C-G-G-T-G-A-T-A) 5'-d(A-T-G-A-G-C-A-C) 5'-d(A-A-T-T-G-T-G-C) 5'-d(T-C-A-T-T-A-T-C-A) 5 '-d(C-C-G-C-C-A-G-A-G) 5 -d (G-T-A-A-A-A-T-A-G-T-C-A) 5'-d(A-C-A-C-G-C-A-C-G-G-T-G) The procedures described in the foregoing examples can also be used for synthesizing mixed nucleoside and deoxynucleoside oligonucleotides by the simple expediency of inserting the desired nucleoside moiety at the desired point in the sequence. Thus, the present process, in addition to being useful for production of oligonucleotides of natural sequence of the individual nucleosides, can be used to produce synthetic oligonucleotides hitherto unknown in natural occurence which can be useful in research and synthesis of polynucleotides, and eventually genes for use in biological systems. A particularly preferred embodiment of the present invention is the detritylation of the 5'-0 trityl nucleosides, deoxynucleotides, oligonucleotides, oligodeoxynucleotides, polynucleotides and polydeoxynucleotides using a Lewis acid, particularly zinc bromide, although other Lewis acids can also be employed, e.g. titanium tetrachloride. The use of Lewis acids is superior to the use of protic acids for removal of the trityl group from the 5'-o- position since reaction is extremely fast and is not accompanied by depurination. The process is also specific for 5'-O-trityl and thus provides a very practical procedure to permit production of 3'-o- trityl blocked nucleosides by reaction with the 3'-0 and 5'-o ditritylated compound. The procedure merely requires contacting the reactants preferably in a reaction solvent and detritylation occurs within short reaction times. The Lewis acid is normally suspended in the reaction solvent and the solvent is usually water-free to prevent reaction with the Lewis acid to form protic acids. In present experience, nitromethane is the preferred solvent although a variety of other solvents can be used, e.g. dioxane and tetrahydrofuran and mixtures of such solvents with each other and other solvents such as acetone, methylene chloride, and the like. The rate of detritylation was measured and comparative data is provided in Table I. TABLE I - The Rate of Detritylation and Depurination of 5' Dimethoxytrityl-N-benzoyldeoxyadenosine Using Various Solutions* Detritylation Depurination Solution Temp Time %' Time satd. ZnBr2,/CH3NO2** 180C < 1 min 100 10 hrs 50 satd. ZnBr2/CH3NO2 0 C 10 min 100 21 hrs < 5 2% toluenesulfonic acid/ 18 C < 1 min 100 5 min 50 CHCl3:CH3OH (7 : 3) 0.5% toluenesulfonic 0 C 10 min 100 8 hrs 50 acid/CHCl3:CH3OH (7 : 3) *All depurination results summarized in this communication were obtained by analyzing reaction mixtures using reverse phase high pressure liquid chromatography. Estimates of detritylation times were by thin layer chromatography. **A nitromethane solution saturated with ZnBr2 is approximately 0.1 M in ZnBr2. The results obtained with various tritylthymidines at room temperature with zinc bromide is give in Table II. TABLE II - The Rate of Detriylation and Degradation of 5' Tityldeoxynucleosides Using ZnBr2 at Room Temperature Detritylation Degradation Deoxynucleoside Time % - Time 5' -Dimethoxytrityl-N- isobutyryldeoxyguanosine < 1 min 100 50 hrs 5' -Dimethoxytrityl-N- benzoyldeoxycytosine < 1 min 100 24 hrs 51-Dimethoxytritylthymidine < 1 min 100 24 hrs and at OOC. in Table III. TABLE III - The Rate of Detritylation of various Tritylthymidinesf Using Saturated ZnBr2 in Nitromethane at OOC. Nucleoside Time % Detritylation 5'-Dimethoxytritylthymidine 1 min 50 5'-Monomethoxytritylthylmidine 1 min 50 5'-Tritylthymidine 10 min 50 3 '-Monomethoxytritylthymidine 30 min 10 The detritylation procedure is not restricted to polymer support synthesis but is also useful for strictly solution synthesis procedures where the use of trityl groups is included in the reaction sequence.

Claims: WHAT IS CLAIMED IS: 1. A modified inorganic polymer represented by the formula: EMI49.1 wherein s is an inorganic polymer linked to the 3' or 5' -0of the nucleoside through a base hydrolyzable covalent bond; R is H or a blocking group;- RI is a hydrocarbyl radical containing up to 10 carbons; each B is a nucleoside or deoxynucleoside base; and each A is H or OR. 2. The modified polymer according to Claim 1 wherein h is lower alkyl. 3. The modified polymer according to Claim 1 wherein the polymer is a silica gel. 4. The modified polymer according to Claim 3 wherein free silanol hydroxy groups on the silica gel are blocked. 5. The modified polymer according to Claim 3 wherein the 5' -0- blocking group is a trityl group. 6. The modified polymer according to Claim 3 wherein the silica gel is macroporous and of a particle size ranging from about 5 to about 1,000 m. 7. A modified inorganic polymer represented by the formula: EMI50.1 whereinOis an inorganic polymer linked to the 3' -0 through an ester linkage; R is H or a blocking group; R1 is a hydrocarbyl radical containing up to 10 carbons, each B is a nucleoside or deoxynucleoside base and each A is H or OR. 8. The modified polymer according to Claim 7 wherein the polymer is a silica gel and A is H. 9. The modified polymer according to Claim 8 wherein free silanol hydroxy groups of the silica gel are blocked. 10. The modified polymer according to Claim 8 wherein the 5' -0- blocking group is a trityl group. 11. The modified polymer according to Claim 10 wherein the trityl group is dimethoxytrityl or monomethoxytrityl 12. The modified polymer according to Claim 8 wherein B is at least one of adenine, guanine, cytosine, uracil and thymine. 13. A modified inorganic polymer comprising an oligonucleotide or deoxyoligonucleotide covalenty bound to said polymer through an ester linkage formed through the 3' -OH of the initial nucleoside moiety, wherein the terminal nucleoside is attached through a phosphite or phosphate triester linkage to the oligonucleotide chain and the 5' -0-substituent is H or a blocking group. 14. The modified polymer according to Claim 13 wherein the polymer is a silica gel. 15. The modified polymer according to Claim 12 wherein the free silanol hydroxy groups on the silica gel are blocked. 16. The modified polymer according to Claim 12 wherein the 5' -0- blocking group is a trityl group. 17. The modified polymer according to Claim 13 wherein the oligonucleotide or deoxyoligonucleotide is attached to said silica gel through the 3' -0- linkage, -Si-Z-NHCO-Z-l-CO- wherein each of Z and Z1 is a hydrocarbyl radical containing up to 10 carbons. 18. A process for production of oligonucleotides which comprises the step of condensing the 3' -OH or 5' -OH group of a nucleoside or oligonucleotide covalently linked to an inorganic polymer by a coupling agent through the 5'-0or 3' -o-, respectively, of said nucleoside or oligonucleotide with a phosphite compound of the formula: EMI51.1 wherein R is a blocking group; B is a nucleoside or deoxynucleoside base; A is H or OR; R1 is lower alkyl; and X is C1 or Br, or a secondary amino group. 19. The process according to Claim 18 wherein the phosphite compound is of the formula: EMI52.1 wherein substituents RtR1, X, A and B are as previously defined and said condensation occurs through the 5' -OH of said nucleoside or oligonucleotide. 20. The process according to Claim 19 wherein R1 is lower alkyl. 21. The process according to Claim 19 wherein the inorqanic polymer is a silica ctel. 22. The process according to Claim 21 wherein A is H and R is a trityl blocking group. 23. The process according to Claim 22 wherein the free silanol hydroxy groups on the silica gel are blocked prior to the condensation reaction. 24. The process according to Claim 22 wherein the trityl group is dimethoxytrityl. 25. The process according to Claim 21 wherein the silica gel is covalently linked to said nucleoside or oligonucleotide through an ester linkage. 26. The process according to Claim 21 including the further step of oxidizing the resulting phosphite triester to a phosphate triester. 27. The process according to Claim 26 wherein I2 is the oxidizing agent. 28. The process according to Claim 26 wherein the 5' -0- blocking group of the product thus obtained is removed and the product is then condensed with a further nucleoside phosphite compound of the said formula. 29. The process according to Claim 28 wherein (1) the product thus obtained is oxidized to obtain the corresponding phosphate triester; (2) the 5t blocking group is removed; and (3) the product is condensed with a further nucleoside or oligonucleoside phosphite of the said formula. 30. The process according to Claim 29 wherein the 5' -0- blocking group is a trityl group and the said group is removed by reaction with a Lewis acid. 31. The process according to Claim 30 wherein the 5' -0- blocking group is a dimethoxytrityl group and said Lewis acid is zinc bromide. 32. The process according to Claim 29 wherein each of steps 1, 2 and 3 is repeated to successively add nucleosides to the oligonucleotide chain. 33. The process according to Claim 32 wherein the oligonucleotide chain comprises from about 10 to about 30 nucleotides attached through the 5' -0 and 3' -0 positions. 34. A process for production of oligonucleotides which comprises the step of condensing a compound of the formula: EMI53.1 wherein B is a nucleoside or deoxynucleoside base, A is H, OH or OR; andes silica covalently bound to the nucleoside by the group -Z1NHCOZCO- wherein Z and Z1 are each alkylene of up to 5 carbon atoms, with a nucleoside phosphite compound of the formula: EMI54.1 wherein A and B are as previously defined; R1 is lower alkyl; R is a blocking group; and X is C1 or Br, or a secondary amino. 35. The process according to Claim 34 including the further step of oxidizing the phosphite triester to a phosphate tries tar. 36. The process according to Claim 35 wherein I2 is the oxidizing agent. 37. The process according tp Claim 35 wherein the 5' -0- blocking group of the product thus obtained is removed and the product is then condensed with a further nucleoside phosphite compound of the said formula. 38. The process according to Claim 37 wherein (1) the product thus obtained is oxidized to obtain the corresponding phosphate triester; (2) the 5' -0 blocking group is removed; and (3) the product is condensed with a further nucleoside phosphite of the said formula. 39. The process according to Claim 38 wherein each of steps 1, 2 and 3 are repeated to successively add nucleosides to the oligonucleotide chain. 40. The process according to Claim 39 wherein the oligonucleotide chain comprises from about 10 to about a0 nucleosides attached through the 5' -0 and 3' -0 positions. 41. The process according to Claim 39 wherein the phosphate triester product is hydrolyzed to form the oligonucleotide and silica gel, and the oligonucleotide is separated therefrom. 42. The process according to Claim 41 including the further step of removing blocking groups from the oligonucleotide. 43. A compound of the formula: EMI55.1 wherein B is a nucleoside or deoxynucleoside base; R is H or a blocking group, A is H or OR; R1 is a hydrocarbyl radical containing up to 10 carbon atoms and X is a secondary amino group. 44. A compound of the formula: EMI55.2 wherein B is a nucleoside or deoxynucleoside base; R is H or a blocking group; A is H or OR: R1,is lower alkyl and X is a secondary amino group formed by removal of the H atom of the secondary amino nitrogen of a nitrogen heterocyclic compound. 45. The compound according to claim 1 or 2 wherein the secondary amino group is tetrazolyl, nitroimidazolyl or triazolyl. 46. The compound according to claim 1 or 2 wherein B is thymine, cytosine, adenosine and guanine. 47. The compound according to claim 1 or 2 wherein R is a trityl group. 48. The compound according to claim 1 or 2 wherein R is di-p-anisylphenylmethyl or p-anisylphenylmethyl. 49. The product according to claim 2 wherein A-is H. 50. An automated apparatus for producing a modified silica gel, comprising: a) first valve means (14) selectively connectable to a supply of active reagents or wash solvents; b) second valve means (28) selectively connectable to a supply nucleoside-active phosphate triester or a wash solvent; c) third valve means (42) selectively connectable to a supply of cleavage reagents or a wash solvent; d) a collector valve (50) for receiving the outflow from, respectively, said first, second and third valve means; e) a closed flow circuit including a selector valve (52) ; a column (10) containing a modified silica gel matrix (12); and iteans (5o) arranged intermediate said selector valve (52) and said column in series connection; said collector valve (SO) Iving the outflow thereof connected to an inlet of said selector valve (52); f) and a uv detector (58) connected to an outlet of said selector valve (52) for conveying said modified silica gel (12) to either a collector or a waste arrangement. 51. The apparatus of Claim 50 comprising programmable controller means (15, 15', 15") connected to each of said first, second and third valve means (14, 2S, 42) for determining the outflow of said valve means to said collector valve. 52. The apparatus of Claim 50 said selector valve being adjustable to direct flow from said collector valve (50) through said column (10) to said uv detector (58) or alternatively, to recycle flow through said column (10). Claims for Austria: ..1. A process for production of oligonucleotides which comprises the step of condensing the 3' -OH or 5' -OH group of a nucleoside or oligonucleotide covalently linked to an inorganic polymer by a coupling agent through the 5'-0or 3' -O-, respectively, of said nucleoside, or oligonucleotide with a phosphite compound of the formula: EMI58.1 wherein R is a blocking group; B is a nucleoside or deoxynucleoside base; A is H or OR; R1 is lower alkyl; and X is C1 or Br, or a secondary amino group. 2,. The process according to Claim 1 wherein the phosphite compound is of the formula: EMI58.2 wherein substituents RtR1, X, A and B are as previously defined and said condensation occurs through the 5' -OH of said,nucleoside or oligonucleotide. 3 The process according to Claim 2- wherein R1 is lower alkyl. .4:. The Drocess accordinq to Claim 2 wherein the inorqanic polymer is a silica qel. 5. The process according to Claim 4. wherein A is H and R is a trityl blocking group. 6. The process according to Claim 5 wherein the free silanol hydroxy groups on the silica gel are blocked prior to the condensation reaction. 7. The process according to Claim 5 wherein the trityl group is dimethoxytrityl. 8-. The process according to Claim 4 wherein the silica gel is covalently linked to said nucleoside or oligonucleotide through an ester linkage. .9'.. The process according to Claim 4 including the further step of oxidizing the resulting phosphite triester to a phosphate triester. 10. The process according to Claim 3 wherein I2 is the oxidizing agent. 11. The process according to Claim 9 wherein the 5' -0- blocking group of the product thus obtained is removed and the product is then condensed with a further nucleoside phosphite compound of the said formula. 12. The process according to Claim 11 wherein (1) the product thus obtained is oxidized to obtain the corresponding phosphate triester; (2) the 5t-t blocking group is removed; and (3) the product is condensed with a further nucleoside or olicnucleoside phosphite of the said formula 13 Thc process according to Claim 12 wherein the 5' -0- blocking group is a trityl group and the said group is removed by reaction with a Lewis acid. 14. The process according to laim 13 wherein the 5' -0- blocking grc-up is a dimethoxytrityl group and said Lewis acid is zinc bromide. 15. The process according to Claim 12 wherein each of steps 1, 2 and 3 is repeated to successively add nucleosides to the oligonucleotide chain. 16, The process according to Claim 15 wherein the oligonucleotide chain comprises from about 10 to about 30 nucleotides attached through the 5' -0 and 3' -0 positions. 17 A process for production of oligonucleotides which comprises the step of condensing a compound of the formula: EMI60.1 wherein B is a nucleoside or deoxynucleoside base, A is H, OH or OR; andOis silica covalently bound to the nucleoside by the group -Z1NHCOZCO- wherein Z and 7 are each alkylene of up to 5 carbon atoms, with a nucleoside phosphite compound of the formula: EMI60.2 wherein A and B are as previously defined; R1 is lower alkyl; R is a blocking group; and X is C1 or Br, or a secondary amino. tR. The process according to Claim 17 including the further step of oxidizing the phosphite triester to a phosphate triester. 19. The process according to Claim 18 wherein I2 is the oxidizing agent. 20. The process according to Claim 18 wherein the 5' -0- blocking group of the product thus obtained is removed and the product is then condensed with a further nucleoside phosphite compound of the said formula. -N1. The process according to Claim20 wherein (1) the product thus obtained is oxidized to obtain the. corresponding phosphate triester; (2) the 5' -0 blocking group is removed; and (3) the product is condensed with a further nucleoside phosphite of the said formula. -2-2. The process according to Claim 21 wherein each of steps 1, 2 and 3 are repeated to successively add nucleosides to the oligonucleotide chain. 23. The process according to Claim 22 wherein the oligonucleotide chain comprises from about 10 to about 30 nucleosides attached through the 5' -0 and 3' -0 positions. 24. The process according to Claim 22 wherein the phosphate triester product is hydrolyzed to form the oligonucleotide and silica gel, and the oligonucleotide is separated therefrom. 25. The process according to Claim 24 including the further step of removing blocking groups from the oligonucleotide. 26. An automated apparatus for producing a modified silica gel, comprising: a). first valve means (14) selectively connectable to a supply of active reagents or wash solvents; b) second valve means (28) selectively connectable to a supply nucleoside-active phosphate triester or a wash solvent; c) third valve means 142) selectively connectable to a supply of cleavage reagents or a wash solvent; d) a collector valve (50) for receiving the outflow from, respectively, said first, second and third valve means; e) a closed flow circuit including a selector valve (52);- a column (10)containing a modified silica gel matrix (12); ; and pfrmp means (56) arranged intermediate said selector valve (52) and said column in series connection; said collector valve (50) having the outflow thereof connected to an inlet of said selector valve (52); f) and a uv detector (58) collected to an outlet of said selector valve (52! for conveying said modified silica gel (12) to either a collector or a waste arrangement. 27. The apparatus of Claim 26 comprising programmable controller means (15, 15', 15") connected to each of said first, second and third valve means (14, 28, 42) for determining the outflow of said valve means to said collector valve. 28. The apparatus of Claim 26 said selector valve being adjustable to direct flow from said collectorvalve (50) through said column (10) to said uv detector (58) or alternatively, to recycle flow through said column (10).

Inventors: CARUTHERS, MARVIN H., MATTEUCCI, MARK D.
Applicants: UNIVERSITY PATENTS, INC.
Application year: 1981
Main classification: C07H21
IPC codes: C07H21, C40B60, C07H23, C07H19, C40B40, B01J19