Z-OMEGA-SUBSTITUTED THIO-2-(2,2-DIMETHYLCYCLOPROPANECARBOXAMIDO)-2-ALKENOIC ACIDS, A PROCESS FOR PREPARING AND AN ANTIBACTERIAL COMPOSITION CONTAINING THE SAME

Abstract: Novel chemical compounds and a process for preparing them are provided. These compounds selectively inhibit the metabolism of dipeptidase (E.C.3.4.13.11) and therefore are useful in combination with antibacterial products. These chemical compounds are Z-2-(2,2-dimethylcyclopropane­ carboxamido)-ω-pyridylthio-2-alkenoic acids (and related arylthio and heterocyclylthio analogs) of the general for­ mula: wherein n is an integer from 3-5, Y is pyridyl, pyrimidinyl, tetrazolyl, imidazolyl, thiadiazolyl, thiazolinyl, or phenyl, optionally having 1 or 2 substituents which are hydroxyl, oxo, carboxyl, or methyl; and the loweralkyl (C₁₋₆) esters and pharmaceutically acceptable salts thereof.

Description: Z-2- ( 2 ,2-DIMETHYLCYCLOPROPANECARBOXAMIDO) ¯L) -SUBSTITUTED THIO-2-ALRENOC ACIDS, A PROCESS -FOR PREPARING AND AN ANTIBACTERIAL COMPOSITION CONTAINING THE SAME INTRODUCTION: A new class of fused ring f3-lactam antibiotics, including thienamycin and its semisynthetic derivatives, epithienamycins, and olivanic acids, has recently been described. These compounds which will be defined more extensively below, are hereinafter referred to as the thienamycin class of compounds11. These compounds have a high level of antibacterial activity, but are subject to extensive metabolism by mammalian species. The kidney was identified as the primary site of metabolism, and an enzyme was' purified from renal extracts which catalyzed the inactivation of thienamycin by hydrolysis of the 13-lactam. By such criteria as cytological localization, substrate specificity and susceptibility to enzyme inhibitors, this enzyme is very similar if not identical to a widely studied renal dipeptidase (E.C.3.4.13.11), also referred to in the literature as dehydropeptidase I". However, the ss-lactamase activity is exhibited only toward the thienamycin class of compounds. Indeed, there exists no precedent example of the mammalian metabolism via ss-lactam cleavage of any representative of the classical n-lactam antibiotics, the penicillins and cephalosporins. DETAILED DESCRIPTION OF THE INVENTION: The chemical substances which selectively inhibit the metabolism of the dipeptidase [E.C.3.4.13.11], also called "dipeptidase inhibitors", include chemical compounds which are substituted 2-alkenoic acids having the following formula EMI2.1 wherein n is an integer from 3-5 and Y is a heterocyclic or phenyl group which may be substituted or unsubstituted. By the termheterocyclic" is meant pyridyl, pyrimidinyl, tetrazolyl, imidazolyl, thiadiazolyl, thiazolinyl, and the like. These rings, and the phenyl ring can be unsubstituted or substituted with hydroxyl, oxo, carbonyl, or methyl. Y-groups include 2-pyridyl; 4-pyridyl; 3-hydroxy-2-pyridyl; 3-carboxy-2-pyridyl; 5-carboxy-2-pyridyl; 2-carboxyphenyl; 1-methyl-1,2,3,4-tetrazol-5-yl; 4-carboxy-6-hydroxy-2-pyrimidinyl; and others. The preferred configuration at the cyclopropyl center is S, although the R,S mixture has been prepared and is active. The most active compounds are those in which n is 4, and Y is 3-carboxy-2-pyridyl, or 3-hydroxy-2pyridyl, in the S-form.t The Z configuration (J.E. Blackwood et al., J. Am. Chem. Soc., 90, p-. 509 (1968)) is assigned to the above compounds on the basis of their NMR spectra by analogy with the work of A. Srinavasan et al. Tetrahedron Letters, 891 (1976). Although these compounds of Formula I are described and named as the free acids, it will be apparent to one skilled in the art that various pharmaceutically acceptable derivatives such as alkali and alkaline earth metal, ammonium, or amine salts, or the like can be employed as equivalents thereto. Salts such as the sodium, potassium, calcium, or tetramethylammonium salts are suitable. UTILITY OF THE INVENTION As noted above, the compounds of this invention are dipeptidase (E.C.3.4.13.11) inhibitors, and can be used in combination with antibacterial compounds which are subject to renal degradation. The group of antibiotics of present primary importance for use in combination with the Z-2-acylamino-3-monosubstituted propenoates of this invention are the "thienamycin class of compounds". The term "thienamycin class of compounds1, is used to identify any of a number of naturally occurring, semi-synthetic, or synthetic derivatives or analog compounds having a common fused-ring n-lactam nucleus. These compounds can be generically classed as 6- and (optionally) 2-substituted pen-2-em-3carboxylic acids and l-carbadethia-pen- 2-em-3carboxylic acids or l-azabicyclo[3.2.Oljhept- 2-ene-7-one-2-carboxylic acids. Specific compounds particularly useful in this invention are represented structurally in the following formula II: EMI4.1 wherein X can be CH2 or S; R2 can be hydrogen; S-alkyl wherein the alkyl group is from 1 to 6 carbons; SCH2CH2NHR3, wherein R3 is hydrogen, acetyl, formimidoyl, acetimidoyl; -s (0) -CH=CHNHCOCH3 and -S-CH=CHNHCOCH3; and Rbis EMI4.2 wherein R7 is hydrogen, hydroxy or sulfonyloxy, or R6 is H. All possible stereoisomeric forms are included within the above structural definition. All of these compounds within Formula II are described in the literature. When X is CH2, and R2 is SCH2CH2NH2, and R6 is CH{OH)CH3, the compound is known as thienamycin, an antibiotic produced by fermentation of S. cattleva, deposited as NRLL 8057 at Northern Regional Research Laboratories, U.S. Department of Agriculture, Peoria, Illinois, U.S.A, on November 18, 1974, described and claimed in U.S. Patent 3,950,357, issued April 13, 1976. The N-substituted derivatives of thienamycin, i.e., in the formula II above wherein R3 is other than hydrogen, are disclosed and claimed in co-pending U.S. applications and their published foreign equivalents. The fermentation product N-acetyl thienamycin (R6 is CH(OH)CH3, .and R3 is acetyl), also called 924A, is claimed in Belgian Patent No. 848,346, issued May 16, 1977. The N-imidoyl derivatives are covered in Belgian Patent No. 848,545, issued May 20, 1977. The unsaturated side chain-containing compound, also called N acetyl-dehydrothienamycin or 924A5 is a fermentation product claimed in U.S. Patent 4,162,323 and also in Belgian Patent No. 866,035, issued October 17, 1978. Epimeric forms of N-acetyl thienamycin, also called 890awl and 890A3, as well as the desacetyl 890A1 and desacetyl 890A3 are disclosed, respectively in published French Patent 76 34887, issued 25 April 1980 and Belgian Patent 848,349, issued 16 May 1977. Epiitencforms forms of the unsaturated thienamycin, also called 890A2 and 890A5 are claimed in published French Patent 77 11891 granted 20 April 1977. The 6-sulfonyloxycontaining N-acetyl compounds, also called.890A9 or 890A10, are claimed respectively, in published French Patent 77 34456, granted 23 June 1980 and published French Patent 77 34457, granted 3 March 1980. Desacetyl analogues of 890asp and 890A 9 10 are disclosed in French Patent 78 03666 granted 5 May 1978; and also in French Patent Application 78 03667, filed 9 February 1978. Some of these latter compounds in the 890at and 890A10 series are also known as derivatives of olivanic acid (see Corbett et al., J. Chem. Soc. Chem. Commun. 1977, No. 24, pp. 953-54). Compounds of the Formula II above when R2 is hydrogen, also called descysteaminyl thienamycins, are disclosed in Belgian Patent 867,227, granted 20 November 1978. When R is hydrogen, and X is CH2, these compounds are disclosed in Belgian Patent 860,962 granted 18 May 1978. A thienamycin type antibiotic in which R2 is SCH2CH2NHAc and R6 is C 2H5, has been named PS5 and is reported by K. Okaimura et al., J. Antibiotics 31 p. 480 (1978), see also Belgian Patent 865,578. The compounds in-which X is S, also called "penems", are described by R.B. Woodward in "Recent Advances in the Chemistry of ss-Lactam Antibiotics", J. Elks (Ed), The Chemical Society,-London, 1977, p. 167; R.B. Woodward, Abstracts of Uppsala University 500 Years Symposium on Current Topics in Drug Research, Uppsala, Sweden, October 1921, 1977. Acta. Pharm. Suecica, Vol. 14, Supplement, p. 23, and U.S. Patent 4,070,477, issued 24 January 1978 and 4,260,618 issued 7 April 1981, and in British Patent applications 2,013,674 published 15 August 1979 and 2,042,520 published 24 September 1980. The compounds wherein X is S are not limited to those elncompassed by formula II above but include, in addition, all of the compounds disclosed in the references listed in this paragraph. The disclosures of.the foregoing patents, pending and abandoned patent applications and literature references are hereby incorporated by reference. Particularly preferred members within the thienamycin class of compounds are the N-formimidoyl and N-acetamidoyl derivatives of thienamycin. The crystalline form of N-formimidoyl thienamycin, which has recently been described, is also useful in the practice of this invention. An example illustrating a preferred way of making this compound follows: ILLUSTRATIVE EXAMPLE N-Formimidoyl thienamycin, (NFT) crystalline Step A. Benzylformimidate hydrochloride A 3 1. three-necked flask fitted with an addition funnel, overhead stirrer, and a reflux condenser, was; charged with a mixture of benzyl alcohol (125 g., 1.15 mol) formamide (51 g., 1.12 mol) and anhydrous ether (1200 ml.). The mixture was stirred vigorously; at room temperature (20-250C) under a nitrogen atmosphere and benzoyl chloride (157 g., 1.12 mol) in 50 ml. of anhydrous ether was added dropwise using the addition funnel. The addition required approximately 50 minutes. The reaction mixture was stirred an additional 60 minutes at room temperature. The ether: was removed by decantation and 300 ml. of acetic anhydride in 500 ml. of anhydrous ether was added. The mixture was stirred 30 minutes at room temperature. The precipitate was allowed to settle and the ether-acetic anhydride was again removed by decantation. The solid was collected by filtration, washed with 500 ml. of ether and dried in vacuo over KOH at 250C for 2 hours to give 130 g. (67%) of benzylformimidate hydrochloride as a white solid. The product was assayed by NMR g(DMSO) 5.7 (s, 2H, wCH2), 7.5 (s, 5H, ), 9.0 (s, lH HC=N). The product is thermally unstable. It decomposes to formamide and benzyl chloride at OOC and above. However, no appreciable decomposition was detected on storage at -200C for 2 months. Step B. Derivatization of Thienamycin Thienamycin (in the form of a 6 1. aqueous solution, pH = 6.5, concentrate from the fermentation broth, containing 28 g. thienamycin) was placed in a large beaker (12 1) and cooled to OOC. The beaker was equipped with a pH meter and an efficient high speed stirrer. The pH was raised to 8.5 by the careful addition of 3N KOH (KOH was added dropwise via syringe to the stirred solution). The solution was treated with 6 equivalents of solid benzyl formirnidate hydrochloride (sol00 g.) in portions while maintaining the pH at 8.5 + 0.3 by the addition- of 3N KOH (200 ml.) using a syringe. The addition required 3-5 min. The reaction mixture was stirred for 6 min. at OOC and then assayed by liquid chromatography to insure completion of the reaction. The solution was adjusted to pH 7 with 1N HCl. The volume of the reaction mixture was measured, and the solution was assayed by UV. The neutralized reaction mixture was concentrated to 15 g./l. on the reverse osmosis unit at < 100C. The volume of the concentrate was measured and the pH was adjusted to 7.2-7.4, if necessary. The concentrate was filtered through a medium porosity sintered glass funnel to remove any solids present after concentration. Step C. Dowex 50W x 2 Chromatography The concentrate (750-1000 ml., 15-20 g.) was applied to OOC. to a precooled 18 1. column of Dowex 50W x 2 in the potassium cycle (200-400 mesh resin) and the column was eluted at 0-50C with distilled deionized water a flow rate of 90 ml/min. and a head pressure of 0-45 psig. Forerun fractions of 4 1., 2 1., and one 1., were collected followed by 18 fractions of 450 ml. each, and one final fraction of 2 1. Each fraction was assayed by UV (1/100 dilution, NH2OH extinction was omitted) and the total amount of NFT present in each fraction was calculated. The beginning and end fractions were assayed for liquid chromatography purity and the desired rich cut fractions were combined. The pH of the combined rich cuts was determined by both pH meter and bromothymol blue indicating solutions and was adjusted to pH 7.2-7.4 if necessary. The combined rich cuts (3-4 1.) were then assayed by UV and the total formamidine content was determined, 15-16 g., 75% yield from the column. The rich cuts were concentrated-on osmosis unit at < 100C as far as possible, then the concentration to 33 g./l. 'was completed on the circulatory evaporator at less than 280C. A total volume of about 500 ml. concentrate was obtained. Step D. Crystallization of N-Formimidoyl Thienamycin The concentrate from the previous step is adjusted to 7.3, if necessary, and N-formimidoyl thienamycin content assayed by UV, was about 85-90%. The concentrate was filtered through a sintered glass funnel (medium porosity) into a large Erlenmeyer flask. Five volumes (or2200 ml.) of 3A ethanol was filtered into the concentrate and the solution was stirred at room temperature for 10 minutes and at OOC for 12-24 hrs. The crystals were filtered by suction filtration and washed with 0.1 volume (N250 ml.) of OOC 80% 3A ethanol followed by 1/25 volume (100 ml.) of 3A ethanol at room temperature. The crystals were dried in vacuo for 12-24 hrs. to give approximately a 40% overall yield of N-formimidoyl thienamycin (10-12 g.). Analytical results on a 50 g. blend of Nformimidoyl thienamycin, prepared as above, are as follows: C, theory 45.42%; found, 45.82% H, theory 6.038; found, 5.72% N, theory 13.24%; found, 13.10% S, theory l0.l08; found, 10.14% residue on ignition, predicted 0.5, found 0.478; 25 [a] = 89.40, T.G. = 6.8%, UV Xmax 300 MM, E% = 328. D METHODS OF USING THE INVENTION As mentioned above, the thienamycintype compound, is used in combination with the dipeptidase inhibitor. The combination of the novel chemical inhibitors of this invention and the thienamycin class compound can be in the form of a pharmaceutical composition containing the two compounds in a pharmaceutically acceptable carrier. The two can be employed in amounts so that the weight ratio of the thienamycin class compound to inhibitor is 1:3 to 30:1, and preferably 1:1 to 5:1. The components can also be separately administered. For instance, the thienamycin class compound can be administered intramuscularly or intravenously in amounts of 1-100 mg/kg/day, preferably 1-20 mg/kg/day, or 1-5 mg/kg/day, in divided dosage forms, e.g., three or four times a day. The inhibitor can be separately administered, orally, intramuscularly, or IV, in amounts of 1-100 mg/kg/day, or preferably 1-30 mg/kg/day, or 1-5 mg/kg/day. The amounts of the two components administered during one day ideally are within the ratio limits denoted above. One preferred dosage form known to applicÅants is as a single dose, of two compounds, one being N-formimidoyl thienamycin and the other being (+) Z-7- (3-carboxy-2-pyridylthio) -2- (2, 2-dimethylcyclopro- panecarboxamido)-2-heptenoic acid, co-administered in a sterile aqueous IV injection form (sodium salt), at a level of 150 mg. of the thienamycin and either 75 or 150 mg of the heptenoic acid. This dose is given to humans (each assumed to weigh about 80 kg.) from l to 4 times a day, or 2-8 mg/kg/day of the thienamycin class compound and 1-8 mg/kg/day of the inhibitor. The thienamycin can also be administered at either 250 or 500 mg., together with the inhibitor at 1:1 (weight) ratio, or 250 or 500 mg also. When this dosage is given 1-4 times daily, from 3,1-25 mg/kg/day is given of each component. The components, whether administered separately or together are employed in pharmaceutically acceptable carriers such as conventional vehicles adapted for oral adminstration such as capsules, tablets, or liquid solutions or suspensions. The components separately or together, can also be dissolved in a vehicle adapted for administration by injection. Suitable formulations for oral use, may include diluents, granulating agents, preservatives, binders, flavoring agents, and coating agents. The example of an oral use composition in the combination of active ingredients, or the acid component alone, intermixed in the dry pulverulent state with gelatin, starch, magnesium stearate, and alginic acid, and pressed into a tablet. As noted above, the presently known preferred method is parenteral administration of the thienamycin class compound and either co-parenteral administration or oral administration of the inhibitor compound. METHODS OF TESTING THE COMBINATION ANTIBACTERIAL AGENT As noted, disposition studies with thienamycin, its natural analogs and its semi-synthetic derivatives have revealed a major metabolic degradation pathway of elimination in the various species examined (mouse, rat, dog, chimpanzee, Rhesus monkey). The extent of metabolism is reflected in low urinary recovery and short plasma half-lives. The nature of this degradation was demonstrated to be lactam cleavage by the renal dipeptidase (E.C.3.4.13.11), described first by Bergmann, M. and Schleich, H., Z. Physiol. Chem., 205 65(1932); see also Greenstein, J.P., Advances in Enzymology, Vol. VIII, Wiley-Interscience, (1948), New York, and Campbell, B.J.; Lin, Y-C., Davis, R.V. and Ballew, E., "The Purification and Properties of Particulate Renal Dipeptidase", Biochim. Biophys. Acta., 118, 371 (1966). In order to demonstrate the ability of the compounds of Formula I to suppress the action of the renal dipeptidase enzyme, an in vitro screen procedure was followed. This measured the ability of compounds to inhibit hydrolysis of glycyldehydrophenylalanine (GDP) by a solubilized preparation of dipeptidase isolated from hog kidneys. The procedure is as follows: to a 1 ml. system containing 50 mM "MOPS" (3-(N-morpholino)propanesulfonic acid) buffer, pH 7.1, is added Sug of lyophilized enzyme, and the test compound at a final concentration of 0.1 mM. After a five minute incubation at 370C, GDP is added to a final concentration of 0.05mM. Incubation is continued for 10 minutes, at 370C and hydrolysis of GDP is measured by the change in optical density with time at 275 nm. Inhibition of the enzyme is gauged by comparison to a standard run containing no inhibitor and is expressed as the inhibitor binding constant, K.. This is the concentration of the inhibitor which achieves 50% inhibition of enzyme. The substrate GDP is employed in preference to thienamycin in this screen because it has a much higher maximal velocity f hydrolysis by renal dipeptidase, thereby reducing the amount of enzyme required. Both GDP and thienamycin have a similar affinity for renal dipeptidase; furthermore, Kits of inhibitors tested have been identicai for the two substrates. Urinary recovery of -thienamycin was measured in all cases with the use of a cylinder or disc diffusion assay, conducted in a manner described in U.S. Patent 3,950,357. This bioassay, with Staphylococcus aureus ATCC 6538 as the test organism, has a useful response range from 0.04 vg/ml to 3.0 Vg/ml. The compounds of this invention are made from an -bromoalkenoic acid. EMI13.1 This is condensed with the appropriate mercaptan, YSH, in water in the presence of sodium bicarbonate at ambient temperature. Most of the desired mercaptans are commercially available. Mercapto nicotinic acids can be prepared by reacting chloronicotinic acids with sodium hydrosulfide according to literature methods, see J. Delarge et al., J. Pharm. Belg., 22, 213 (1967); C.A. 68, 59406 (1968); and L. R. Fibel et al., JACS 70, 3908; (1948). More detail about preparation of the compounds is found in the following examples. PREPARATIVE EXAMPLE Z-8-Bromo-2-(2,2-Dimethylcyclopropanecarboxamido)-2- octenoic acid To a suspension of 14.4 g (0.3 mole) of 50% NaH dispersion in 360 ml of toluene cooled in an ice bath and in a N2 atmosphere was added over 45 minutes a solution of 146 g (0.6 moles) of 1,6-dibromohexane and 57.6 g (0.3 mole) of ethyl 1,3-dithiane-2-carboxylate in 120 ml of DMF. The cooling bath was removed and the mixture stirred at room temperature for -20 hours. The reaction mixture was washed with water (3 x 210 ml), dried over MgSO4 and evaporated under reduced pressure to give 179.5 g of a yellow oil containing the desired alkylated dithiane, 1,6-dibromohexane and mineral oil. This crude material was used in the next reaction without purification. To a suspension of 426 g (2.4 moles) of N-bromosuccinamide in 800 ml of acetonitrile and 200 ml of H2O was added over 45 minutes a solution of the crude dithiane in 100 ml of acetonitrile. The temperature of the reaction mixture was maintained below 250C with an ice bath. After stirring at 200C for 10 minutes the dark red reaction mixture was poured into 2 1. of hexane-CH2Cl2 (1:1). The solution was shaken with saturated NaHSO3 (2 x 460 ml) and water (1 x 500 ml). Then 400 ml of saturated Na2CO3 solution was added in small portions (vigorous CO2 solution). After the foaming subsided the funnel was shaken and the aqueous phase separated. The organic layer was extracted with saturated Na2CO3 solution (400 ml) and water (500 ml) and dried over MgSO4. Removal of the solvent under reduced pressure gave 133.8 g of crude bromo ketoester containing 1,6--dibromohexane and mineral oil. This crude material was used in the next reaction without purification. A mixture of 133.8 g of crude bromo ketoester, 133 ml of 50% hydrobromic acid and 267 ml of acetic acid was heated at 900C (internal temperature) for 75 minutes. The dark solution was evaporated under reduced pressure until most of the acetic acid was removed. The residue was dissolved in 500 ml of ether, washed with water (2 x 100 ml) and extracted with saturated NaHCO3 (3 x 200 ml). The combined NaHCO3 extracts were extracted with ether (2 x 100 ml) and acidified with concentrated HCl. The precipitated oil was extracted with ether (3 x 200 ml). The ether extracts were washed with water (1 x 100 ml) and saturated brine (1 x 100 ml) and dried over MgSO4. Removal of the ether under reduced pressure gave 46.2 g of pure bromo keto acid, homogeneous by TlC (silica gel, 4:1 toluene-acetic acid). The NMR spectrum was consistent with the desired product. A mixture of 46.1 g (0.194 moles) of the bromo keto acid , 17.6 g (0.156 mole) of 2,2-dimethylcyclo propanecarboxamide and 450 ml of toluene was heated under reflux for 13 hours, with collection of water in a small Dean-Stark trap. After cooling, the clear reaction mixture was extracted with saturated NaHCO3 solution (4 x 100 ml). The combined extracts were washed with ether (2 x 100 ml) and then the pH was adjusted to 3.5 (pH meter) by addition of concentrated HCl. An oil precipitated: which soon crystallized. The solid was filtered, washed well with water and dried. Recrystallization from acetonitrile gave 22.5 g of Z-8-bromo-2-(2,2 dimethylcyclopropanecarboxamido) -2-octenoic acid, m.p. 151-1530C, homogeneous by TLC (4:1 tolueneacetic acid). The NMR spectrum was consistent with the desired structure. Anal. (C14H22BrNO3) Calcd Found C 50.61 50.66 H 6.67 6.96 N 4.22 4.45 Br 24.05 23.95 The following cJ-bromo-compounds were prepared using the same procedure: Z-6-Bromo-2- (2,2-dimethylcyclopropanecarboxamido) -2- hexenoic acid; Z-7-Bromo-2-(2,2-dimethylcyclopropanecarboxamido) -2- heptenoic acid. The compound (+)Z-7-bromo-2-(2,2-dimethylcyclopropane carboxamido)-2-heptenoic acid, has a melting point of 86-880C, and optical rotation, 28 [a] = +69.90. D Example 1 Racemic Z-7-(3-hydroxy-2-pyridylthio) -2-(2,2-dimethyl- cyclo-propanecarboxamido)-2-heptenoic acid A solution of 200 mg (0.630 mmole)-:of Z-7-bromo-2 (2, 2-dimethylcyclopropanecarboxamido) -2-heptenoic acid, 72.4 mg (0.57 mmole) of 2-mercapto-3-pyridinol, and 134 mg (1.26 mmole) of Na2CO3 in l.0 ml of H 0 was stirred at room temperature under nitrogen for 3 days. The reaction mixture was partitioned between 10.0 ml of 1.5 N HCl and 10.0 ml Et2O. The aqueous fraction was adjusted to pHN3.5 with 2.5 N NaOH. The product did not oil out, but was extracted into Et2O, dried with MgSO4, and filtered. Concentration of the solution gave 151.0 mg of white crystalline product. This product was recrystallized from nitromethane with several drops of acetic acid, yield 43.0 mg; m.p.=199.0-200.50C. and analysis gave for C18H23N2O4S.0.5 H2O, MW=373.46: found calculated N 7.21 7.50 C 58.17 57.89 H 6.72 6.75 S 8.70 8.59 EXAMPLE 2 (+)'-Z-2- (2, 2-Dimethylcyclopropanecarboxamido) -7- (3- hydroxy-2-pyridylthio) -2-heptenoic acid A mixture of 2.00 g (6.3 mmole') of (+) -Z-7-bromo-2- (2, 2-dimethylcyclopropanecarboxamido) - 2-heptenoic acid, 0.73 g (5.75 mmole) of 2-mercapto-3-pyridinol, 1.34 g (12.6 mmole) of Na2CO3, and 8 ml of H 29 was stirred at room temperature under N2. After 17.5 hours, the mixture was treated cautiously with 60 ml of 2.5 N HCl (foaming) and shaken with 60 ml of' in a separatory funnel until all of the gummy precipitate obtained initially upon acidification had dissolved in one phase or the other. The aqueous phase was adjusted to pH^S3-3.5 with 2.5 N NaOH, causing separation of a gum. The mixture was heated on a steam bath, resulting in solidification. After cooling, the solid was collected on a filter and washed with H2. The air-dried material (1.7 was suspended in rev50 ml of nitromethane, heated on a steam bath, and treated slowly with AcOH while hot until all of the solid dissolved. The solution was treated with charcoal and filtered through Super-Cel. The filtrate was reheated to redissolve crystallized product and then allowed to cool slowly. After standing, the crystallized product was collected on a filter and washed with nitromethane. The material was finally dried in a vacuum oven at 1000. Yield of white crystals = 1.24 g (59%, m.p. 188-189 . Tlc (2:1 toluene-AcOH) was virtually homogeneous. NMR(DMSO-d6) was consistent with the assigned structure. From the mother liquor there was obtained a second crop of light cream-colored crystals: 0.12 g, m.p. 185-186 ; tlc similar to that of first crop. Total yield of material = 1.36 g (65%), ki=0.02; 27 [a] = +343O D Anal. (C18H24N2O4S) Calcd. Found (lust crop) C 59.32 59.28 H 6.64 6.58 N 7.69 7.58 S 8.80 ¯ 8.96 EXAMPLE 3 Racemic Z-7-(3-carboxy-2-pyridylthio)-2-(2,2 dimethylcyclopropanecarboxamido) -2-heptenoic acid A- solution of 108.0 mg (0.34 mmole) of Z-7-bromo-2- (2, 2-dimethylcyclopropanecarboxamide) -2- heptenoic acid, 48.0 mg (0.31 mmole) of 2-mercaptonicotinic acid, and 72.1 mg (0.68 mmole) of Na2CO3 in 0.5 ml H2O was stirred under N2 at room temperature. After 1 day, the reaction mixture was partitioned between 2.5 N HCl and Et2O. The product was extracted into 2.5 N Hdl and to this layer was added 50% NaOH to pH 2.5-3.0. The product was extracted into Et2O, dried with MgSO4, filtered, and concentrated on the rotovac to yield 51.0 mg (38%) with purity confirmed by NMR and tlc., ki = 0.p4. EXAMPLE 4 Racemic Z-7-(5-carboxy-2-pyridylthio)-2-(2,2 dimethylcyclopropanecarboxamido) -2-heptenoic acid A solution of 108.0 mg (0.34 mmole) of Z-7-bromo-2- (2, 2-dimethylcyclopropanecarboxamide) -2- heptenoic acid, 48.0 (0.31 mmole) of 6-mercaptonicotinic acid and 72.0 mg (0.68 mmole) of Na2CO3 in 0.5 ml of H2O was stirred under N2 at room temperature. After 5 days, the reaction mixture was partitioned between 5.0 ml of Et2O and 5.0 ml of 2.5 N HCl. To the aqueous layer was ad-ded 10% NaOH until the solution was cloudy white, approx. pH 2-3. The product then extracted into Et2O. The Et2O layer was dried with MgSO4 and concentrated on the rotovac to yield 95.0 mg (71%) of white glassy solid confirmed by NMR, tlc (8:1 toluene:AcOH); m.p. > 600C (after drying under high vacuum), ki = 0.25. Analysis: C19H24N205S MW=392.47, Calcd. for 0.25 H2O found C 57.49 57.78 H 6.22 6.42 N 7.06 6.58 EXAMPLE 5 (+)-Z-7-(3-carboxy-2-pyridylthio)-2-(2,2-dimethyl cyclopropanecarboxamido)-2-heptenoic acid A solution of 600.0 mg (1.89 mmole) of (+) Z-7-bromo-2-(2,2-dimethylcyclopropanecarboxamido) -2- heptenoic acid, 267.0 mg (1.72 mmole) of 2-mercaptonicotinic acid and 400.6 mg (3.78 mmole) of Na2CO3 in 3.0 ml of H2O was stirred under N2 at room temperature. After 3 days, the reaction was partitioned between 25.0 ml. of 2.5 N HCl and 25.0 ml of Et2O. The Et2O layer was partitioned with an additional 25.0 ml of 25 N HCl. To the aqueous fractions was added 10% NaOH until the solution turned cloudy white (still pH (l (1.0). Extraction into Et2O, drying with MgSO4, filtration and concentrated on rotovac gave a pale yellow glassy solid, 394.0 mg, m.p. > 700C. Analysis for ClgH24N205S M5=392.47 calcd. for 0.25 H2O found C 57.49 57.73 H 6.22 6.55 N 7.06 -6.77 EXAMPLES 6-13 Using procedures similar to those of Examples 1-5, the following compounds were made, characterized, and tested. Example 6 Z-2-(2,2-dimethylcyclopropanecarbox amido) -6-(3-hydroxy-2-pyridylthio) -2-hexenoic acid; white glassy solid, m.p. = > 700C; analysis calcd. for Cl7H22N2o4S.0.25 H20: C, 57.53; H, 6.39; N, 7.89; found: C, 57.54; H, 6.67; N, 7.93. Example 7 Z-7-(2-pyridylthio)-2-(2,2-dimethylcyclopropanecarboxamido)-2-heptenoic acid; tlc and NMR good. Example 8 Z-7-(4-pyridylthio)-2-(2,2-dimethylcyclo- propanecarboxamido)-2-heptenoic acid; a glassy oil; analysis for C18H23N2O45. 3/4 H2O; calc.: C, 59.90; H, 6.76; N, 7.76; found: C, 59.95; H, 6.99; N, 7.01. Example 9 Z-7-(2-carboxyphenylthio)-2-(2,2-dimethyl cyclopropanecarboxamido)-2-heptenoic acid; m.p. 163-1670C, decomposition; anal. for -' C20H25NO5S.0.75 H2O; calc.: C, 59.32; H, 6.60; N, 3.45; found: C, 59.25; H, 6.36; N, 3.26. Example 10 Z-2-(2,2-dimethylcyclopropanecarboxamido)- 7-phenylthio-2-heptenoic acid; m.p. l00-1040C, dec. anal. for C19H25NO3S.0.75 H2O; calc.: C, 63.22; H, 7.40; N, 3.88; found: C, 63.05; H, 7.23; N, 3.90. Example 11 (+)-Z-2-(2,2-dimethylcyclopropane- carboxamido)-8-(3-hydroxy-2-pyridylthio)-2-octenoic acid, a white gummy solid; analysis shows C19H26N2O4S. 1/2 H2O. Example 12 Z-8-(l-methyl-5-tetrazolylthio)-2-(2,2 dimethylcyclopropanecarboxamido) -2-octenoic acid; a viscous oil; also made as the t-butyl ester and the sodium salt. Example 13 Z-8-(4-carboxy-6-hydroxy-2-pyrimidinyl- thio)-2-(2,2-dimethylcyclopropanecarboxamido-2-octenoic acid. Example 14 Z-8-(2-thiazolinylthio)-2-(2,2-dimethylcyclopropanecarboxamido)-2-octenoic acid, m.p. 86-900C, ki=0.15, C17H26N2O35 2 calc.: C = 55.11; H, 7.07; N = 7.56; S = 17.31; found: C = 54.61; H, 7.20; N = 7.36; S = 17.21.

Claims: WHAT IS CLAIMED IS: 1. The compound of the following formula: EMI24.1 wherein n is an integer frbm 3-5, Y is pyridyl, pyrimidinyl, tetrazolyl, imidazolyl, thiadiazolyl, thiazolinyl, or phenyl, optionally having 1 or 2 substituents which are hydroxyl, oxo, carboxyl, or methyl; and the loweralkyl (C1-6) esters and pharmaceutically acceptable salts thereof. 2. The compound of Claim 1 wherein Y is pyridyl or phenyl. 3. The compound of Claim 2 in which the substituent is hydroxyl or carboxyl. 4. The compound of Claim 1 which is Z-7- (3-hydroxy-2-pyridylthio) -2- (2,2-dimethylcyclo- propanecarboxamido)-2-heptenoic acid. 5. The racemic (+) form of the compound of Claim 4. 6. The S(+) form of the compound of Claim 4. 7. The compound of Claim 1 which is Z-7-(3-carboxy-2-pyridylthio)-2-(2,2-dimethylcyclopro- panecarboxamido)-2-heptenoic acid. 8. The racemic (+) form of the compound of Claim 7. 9. the S(+) form Of the compound of Claim 7. 10. The compound Of Claim 1 which is Z-(5-carboxy-2-pyridylthio) (2,2-dimethylcyclopropane carboxamido)-2-heptenoic acidr 11. An antibacteridl composition comprising a combination of thienamycin-type compound and a dipeptidase (E. C. 3. 4. 13. 11) inhibitor which is the compound defined in Claim 1, the ratio of the thienamycintype compound to the dipeptidase inhibitor being within the range of about 1:3 to about 30:1. 12. The composition of Claim 11 in which the thienamycin-type compound is thienamycin, N-formimidoyl thienamycin,-or N-acetimidoyl thienamycin. 13. The process of preparing the compounds of Claim 1 which comprises reacting a # -bromo alkenoic acid of the formula: EMI26.1 wherein n is as defined in Claim l; with a mercaptan of the formula: YSH wherein Y is as defined in Claim 1; in water in the presence of sodium bicarbonate, at ambient temperature, and recovering the product thereby produced. Claims for Austria 1. Process of preparing compounds of the following formula: EMI27.1 wherein n is an integer from 3-5, Y is pyridyl, pyrimidinyl, tetrazolyl, imidazolyl, thiadiazolyl, thiazolinyl, or phenyl, optionally having 1 or 2 substituents which are hydroxyl, oxo, carboxyl, or methyl; and the ioweralkyl (C1-6) esters and pharmaceutically acceptable salts thereof, which comprises reacting ao-bromo alkenoic acid of the formula: EMI27.2 with a mercaptan of the formula: YSH in water in the presence of sodium bicarbonate, at ambient temperature, and recovering the product thereby produced. 2. Process according to Claim 1 for preparing compounds wherein Y is pyridyl or phenyl. 3. Process according to Claim 1 for preparing compounds of Claim 2 in which the substituent is hydroxyl or carboxyl. 4. Process according to Claim 1 for preparing Z-7-83-hydroxy-2-pyridylthio)-2-(2,2- dimethylcyclopropanecarboxamido) -2-heptenoic acid. 5. Process according to Claim 1 for preparing the racemic (t) form of the compound of Claim 4. 6. Process according to Claim 1 for preparing the S (+) form of the compound of Claim 4. 7. Process according to Claim 1 for preparing Z-7-(3-carboxy-2-pyridylthio)-2-(2,2 dimethylcyclopropanecarboxamido) -2-heptenoic acid. 8. Process according to Claim 1 for preparing the racemic (+) form of the compound-of Claim 7. 9. Process according to Claim 1 for preparing the S (+) form of the compound of Claim 7. 10. Process according to Claim 1 for preparing Z-(5-carboxy-2-pyridylthio)-2-(2,2 dimethylcyclopropanecarboxamido) -2-heptenoic acid.

Inventors: ASHTON, WALLACE T., BARASH, LOUIS, BROWN, JEANNETTE E., GRAHAM, DONALD W.
Applicants: MERCK & CO. INC.
Application year: 1981
Main classification: C07D213
IPC codes: C07D257, A61K31, A61P31, C07D233, A61P43, C07D277, C07D285, C07C313, C07D213, C07C67, C07C323, C12N9, C07D477, C07D239