PHARMACEUTICALLY ACCEPTABLE SALTS OF 4'-(9-ACRIDINYLAMINO) METHANESULFON-M-ANISIDIDE, THEIR PRODUCTION, AND PHARMACEUTICAL COMPOSITIONS CONTAINING SUCH SALTS

Abstract: For use as, inter alia, an anti-neoplastic agent, there is disclosed a compound of the formula:- wherein X is an acid having a total of from three to six carbon atoms, two to five hydroxy groups and an acidic group selected from -CO₂H and -OPO₃H₂ with the proviso that, when the total number of carbon atoms is three or four, the acidic group is -OPO₃H₂. These particular salts of 4′-(9-­ acridinylamino)methanesulfon-m-anisidide remain in solu­ tion for a reasonable period and hence permit the use of the compound in this salt form for introduction into a mammal. Also disclosed are the processes for producing the salts and pharmaceutical compositions including the salts.

Description: "PHARMACEUTICALLY ACCEPTABLE SALTS OF 4'-(9-ACRIDINYL AMINO)METHANESULFON-m-ANISIDIDE, THEIR PRODUCTION, AND PHARMACEUTICAL COMPOSITIONS CONTAINING SUCH SALTS" This invention relates to pharmaceutically acceptable salts of 4'-(9-acridinylamino)-methanesulfonm-anisidide, to processes for their production, to a pharmaceutical composition including such salts, and to the use of such salts and composition as antineoplastic agents. The compound 4'-(9-acridinylamino)methanesulfon-m-anisidide (hereinafter abbreviated to m-AMSA) has the following formula: EMI1.1 According to one aspect of the present invention, there is provided a compound having the following general formula: - EMI2.1 wherein X is an acid having a total of from three to six carbon atoms, two to five hydroxy groups and an acidic group selected from -CO2H and -OPO3H2 with the proviso that, when the total number of carbon atoms in the acid is three or four, the acidic group is -OPO3H2. The compounds of the present invention are salts having a relatively high degree of solubility in water and capable of remaining in solution for a sufficient period of time to permit the solution to be introduced into a mammal. The term "solution" is used in this specification to mean a material which is in a liquid state capable of completely or almost completely passing through a 0.22 p millipore filter. Certain of the salts of the present invention do form true solutions; however, this may not always be the case. The present invention thus provides pharmaceutically acceptable acid-addition salts of the formula m-AMSA X wherein X is as defined above. The acid X of the formula m-AMSAX, may be in the D,L or a racemic form. In addition, the salt m-AMSAX may exist in a solvated form. All of the foregoing forms are intended to be encompassed by the formula m-AMSAX. The preferred acids represented by X are D-gluconic acid, D-glucuronic acid, Dgalacturonic acid and B-glycerophosphoric acid which give rise to 4'-(9acridinylamino)methanesulfon-m-anisidide D-gluconate, 4'-(9-acridinylamino)methanesulfon-m-anisidide D-glucuronate, 4'-(9-acridinylamino)methanesulfon-manisidide D-galacturonate, and 4l-(9-acridinylamino)- methanesulfon-m-anisidide -glycerophosphate, respectively. The present invention also relates to methods for preparing said salts, pharmaceutical compositions utilizing said salts and methods of treating mammals having certain viral or microbial infections, cancer or a tumor with said salts, preferably in the form of the disclosed pharmaceutical compositions. The compound 4 '-(9-acridinylamino)methanesulfon-m- anisidide, -generally referred to as m-AMSA, has been found to have a high degree of activity in treating mammals in studies using mice: "The Experimental Antitumour Properties of Three Congeners of the Acridylmethanesulphonanilide (AMSA) Series", B. F. Cain and G. J. Atwell, Europe. J. Cancer, Vol. 10, pp. 539-549. Pergamon Press (1974); "Potential Antitumor Agents. 16. 4'-(Acridin-9- ylamino)methanesulfonanilides", Bruce F. Cain, Graham J. Atwell and William A. Denny, Journal of Medicinal Chemistry,- Vol. 18,- pp. 1110-1117 (1975); "Structure-Activity Relationships for Thiolytic Cleavage Rates of Antitumor Drugs in the 4'-(9 Acridinylamino)methanesulfonanilide Series Bruce F. Cain, William Robert Wilson and Bruce C. Baguley, Molecular Pharmacology, Vol. 12, pp. 1027-1035 (1976); "Potential Antitumor Agents. 17. 9-Anilino-10- methylacridinium Salts", Bruce F. Cain, Graham J. Atwell and William A. Denny, Journal of Medicinal Chemistry, Vol. 19, pp. 772-777 (1976); "Potential Antitumor Agents. 26. Anionic Congeners of the 9-Anilinoacridines", William A. Denny, Graham J. Atwell and Bruce F. Cain, Journal of medicinal Chemistry, Vol. 21, pp. 5-10 (1978). The compound m-AMSA is generally prepared by coupling 9-chloroacridine to 4'-aminomethanesulfon-m-anisidide under acid-catalyzed conditions which is described in the following references: "Potential Antitumor Agents. 12. 9-Anilinoacri dines", G. J. Atwell, B. F. Cain and R. N. Seelye, Journal of Medicinal Chemistry, Vol. 15, pp. 611-615 (1972); "The Experimental Antitumour Properties of Three Congeners of the Acridylmethanesulphonanil ide (ANSA) Series", B. F. Cain and G. J. Atwell, Europ. J.-Cancer, Vol. 10, pp. 539-549. Pergamon Press (1974); "Potential Antitumor Agents. 16. 4'-(Acridin-9- ylamino)methanesulfonanilides.", Bruce F. Cain, Graham J. Atwell and William A. Denny, Journal of Medicinal Chemistry, Vol. 18, pp. 1110-1117 (1975); "Potential Antitumor Agents. 17. 9-Anilino-lO methylacridinium Salts", Bruce F. Cain, Graham J. Atwell and William A. Denny, Journal of Medicinal Chemistry, Vol. 19, pp. 772-777 (1976); "Potential Antitumor Agents. 20. Structure Activity-Site Relationships for the 4'-(9 Acridinylamino)alkanesulfonanilides", Bruce F. Cain and Graham J. Atwell, Journal of Medicinal Chemistry, Vol. 19, pp. 1409-1416 (1976); "Potential Antitumor Agents. 23. 4'-(9-Acridinyl amino) alkanesulfonanilide Congeners Bearing Hydrophilic Functionality", Bruce F. Cain, Graham J. Atwell and7William A. Denny, Journal of Medicinal Chemistry, Vol. 20, pp. 987-996 (1977). which are incorporated by reference. Unfortunately, m-AMSA is a compound which is not water soluble. It also has been found that the salts of m-AMSA, such as the hydrochloride salt, are also of a low order of solubility in water (see Table I). If the compound is to be administered parenterally, a solution containing the compound must-be prepared. At present, solutions employing dimethylacetamide as the solvent have been tried; however, the toxicity associated with this solvent makes this approach very undesirable. In addition, the dimethylacetamide is a potent solvent which presents problems as relates to the tubing and. plastic syringes used when administering or storing the solution. It has been found that certain acids when reacted with m-AMSA give salts having high degrees of water solubility. Depending upon the salt, concentration and temperature, standing may result in certain of the solutions-of sal-ts forming -gels -However, a- salt need only remain in solution for a period long enough for it to be administered to a patient. The stability of aqueous solutions of certain preferred compounds is given in Table II. TABLE I Relative Solubility of m-AMSA Salts a,b Acid Rel. Solubility as Comp. to H20 Galacturonic 6133 Glucose-6-Phosphoric 3785 Gluconic 2815 Ascorbic 1213 ss-Glycerophosphoric 1085 Glucuronic 923 Lactic 890 Glyceric 555 Citric 438 Acetic 234 Propionic 225 Gallic 225 Isethionic 213 Malonic 192 D,L-Malic 165 Succinic 154 d-Tartaric 138 Glutaric 102 Mucic 33 Phosphoric 29 Salicylic 18 Glycolic 18 Benzoic 10 Methane-Sulfonic 7.5 Gentisic 7.5 Sulfuric 6 Nitric 3 Sulfamic 2.8 Hydrochloric 1.2 Water 1 a) After solution is achieved, certain solutions upon standing set up like gels. The fact that on standing gels form in certain instances is not reflected in the table. b) The values were obtained by adding m-AMSA to 0.1 M aqueous solutions, except gallic acid (O.05M), mucic acid (0.01M), salicylic acid (0.015M), benzoic acid (0.015M) and gentisic (O.03M). TABLE II Stability of Preferred m-AMSA Salts m-AMSA Concentration Temperature m-AMSA Salt mg/ml C Results gluconate 5.2 room temperature a) after four days - fluid and clear to tyndall light. b) after three weeks - fluid and clear to ordinary light, haze by tyndall light. gluconate 5.2 5 a) after four days - solution gelled. gluconate 7.4 room temperature a) after three days fluid and clear to tyndall light. b) after three weeks - fluid and clear to ordinary light, haze by tyndal light. gluconate 7.4 5 a) after three days - solution gelled gluconate 10 room temperature a) after 18 hours - fluid and clear to tyndall light. b) after 18 days - clear to ordinary light, haze by tyndall light. gluconate 10 5 a) after 18 hours - solution gelled. b) after 18 days - solution gelled* with slight flocculant precipitate. *all foregoing gluconate solution of lower concentration that gelled at 5 C could be returned to a fluid state by warming to room temperature ans shaking, at 10 mg/ml this could not be achieved after storing at 5 C. TABLE II (Continued) Stability of Preferred m-AMSA Salts m-AMSA Concentration Temperature m-AMSA Salt mg/ml C Results glucoronate 4.97 room temperature a) after 18 days - hazy to tyndall light and some particles. glucoronate 4.97 5 a) after 18 days - solution gelled but liquifies on warning to room temperature with stirring (haze to tyndall light and slight precipitate). ss-glycerophosphate 7.5 room temperature a) after three days - fluid and clear to tyndall light. b) after three weeks - solution gelled but became fluid on shaking (haze to tyndall light). ss-glycerophosphate 7.5 5 a) after three days - gelled (warming to room temperature and shaking gave solution clear to tyndall light). b) after three weeks - gelled (warming to room temperature ans shaking gave solution hazy to tyndall light). It is also noted that solubility is enhanced by the presence of an excess of an acid of the formula X' wherein X' is an acid having a total of from three to six carbon atoms, two to five hydroxy groups and an acidic group which may be a -CO2H group or a -OPO3H2 group with the proviso that when the total number of carbon atoms is three or four, the acidic group is OPO3H2. The acid X' may or may not be the same as the acid used in forming the salt of m-AMSA. Generally, the same acid is employed to form the salt of m-AMSA and supply the excess acid, since it is more convenient to use this approach to prepare the m-AMSA salt with excess acid (X and X' are the same in this instance). The quantity of excess acid used, when compared to the m-AMSA salt on a molar basis, is about 0.2 moles to about 10.0 moles per mole of m-AMSA salt, preferably approximately 1.0 moles to approximately 6.0 moles. The salts are prepared from m-AMSA base and the acid in an inert solvent. Although up to 10% molar excess of either reagent may be employed, equimolar proportions of m-AMSA base and the acid are preferred. The m-AMSA base and the acid are dissolved in dimethylformamide, dimethylacetamide, methanol, anhydrous ethanol, 95% ethanol, isopropanol or combinations of the solvents at 35 to 100 C. After filtration of the solution, the salt is precipitated by addition of another solvent such as anhydrous ethanol, isopropanol, anhydrous diethyl ether or ethyl acetate and/or chilling at 5- to -20 C for 2 to 24 hours. The preferred method is to dissolve the m-AMSA base and acid in anhydrous ethanol and dimethylformamide at 70-10O, in boiling anhydrous ethanol or in boiling methanol. The solution is filtered and the salt separated from the filtrate by the following procedures. The dimethylformamide-anhydrous ethanol solution (about 70"C) is diluted with anhydrous ethanol; the. anhydrous ethanol and the methanol solutions at 30 35-C are diluted with diethyl ether. The product is further precipitated by. chilling the filtrates at 5' to -20'C for 16-24 hours. After collecting the product it is dried under reduced pressure at 50-60'C. The compounds of the present invention are useful in treating certain viral infections, namely those caused by Avian myeloblastoma or vaccinia virus or certain bacterial infections, namely those caused by Salmonella tyDhimurium bacteria. In addition, compounds of the present invention are useful antineceplastic agents in the treatment of mammals, such as dogs, cats, etc. Typical of the disease states wherein the compounds of this invention may be employed are leukemia, breast cancert cellular hepatoma, melanoma, etc. The preferred route of administration is intravenous and the dose generally employed is from about 20 mg to about 500 mgZm2 of body surface per day for one to five days preferably 30 mg to 100 mg/m2 per day for about three days. The procedure may be repeated about every three weeks. The compounds of the present invention may also be administered orally in the form of capsules, tablets, syrups, etc., or rectally in the form of suppositories Generally, higher doses are employed when using these routes, especially orally. Thus, when employing these routes about 20 mg to about 1.0 g/m2 of body surface per day for from one to -five days, preferably 50 mg to 800 mg/m2 per day for about three days is used. The compounds of the invention, when administered intravenously, are generally dissolved in water, sterifiltered, lyophilized and redissolved at the time of use. Preferably, an excess of acid is present to further improve solubility and minimize the chances of any blockage of the pores.during the sterifiltration step. An excess of an acid is less desirable if the sterilization process does not utilize a microfiltration step. The lyophilized material is dissolved in a non-toxic aqueous vehicle prior to administration. The vehicle may contain buffers, materials, to make the resultant solution isotonic, preservatives, etc., which are compatable with the compounds of this invention. Certain ions, such as halide ion, cause precipitation of the hydrohalide salt of m-AMSA and should be avoided. In addition, the compounds of the present invention are orally administered, for example, with an inert diluent or with an assimilable edible carrier, or they may be compressed into tablets, or they may be incorporated directly with the food of the diet. For oral therapeutic administration, the active compounds of this invention may be incorporated with excipients and used in the form of tablets, troches, capsules, elixirs, suspensions, syrups and the like. Such compositions and preparations should contain at least 0.18 of active compound. The percentage in the compositions and preparations may, of course, be varied and may conveniently be between about 5% to about.758 or more of the weight of the unit. The amount of active compound in such therapeutically useful compositions or preparations is such that a suitable dosage will be obtained. Preferred compositions or preparations according to the present invention are prepared so that an oral dosage unit form contains between about 10 and 200 milligrams of active compound. The tablets, troches, pills, capsules and the like may also contain the following: a binder such as gum tragacanth, acacia, corn starch or gelatin; an excipient such as dicalcium phosphate, a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin may be added or a flavoring agent such oil of wintergreen, or cherry flavoring. Various bther materials may be present as coatings or to otherwise modify the physical form of the dosage unit, for instance, tablets, pills or capsules may be coated with shellac, sugar or both. A syrup or elixir may contain the active compounds, sucrose as a sweetening agent, methyl and propyl parabens as preservatives, a dye, and a flavoring such as cherry or orange flavor. Of course, avy material used in preparing any dosage unit form should be pharmaceutically pure and substantially non-toxic in the amounts employed. Lastly, the compounds of this invention may be administered in the form of a suppository using glycerin, cocoa butter, etc., as a vehicle. In addition to the compounds of the invention, preseratives and/or coloring agents may also be incorporated into the suppository. The enhanced solubility of the m-AMSA salts may also give. certain advantages when administered by these routes. The compounds of this invention may be administered in combination with other antineoplastic agents. The antineoplastics may be derived from the numerous classes of agents; such as: antibiotic derivatives, doxorubicin, mitomycin, actinomycin, etc.; antiestrogen agents, such as tamoxifen; antimetabolites, such as fluorouracil, carmustine, lomustine, methotrexate, mercaptopurine, thioguanine, etc. cytotoxic agents, such as bleomycin, cyclophosphamide, busulfan, procarbazine, hyroxyurea, etc.; hormones, such as dromostanolone, ethinyl estradiol, megestrol, prednisone, methyltestosterone, chlorotrianisene, testolactone, etc.; nitrogen mustard derivatives, such as melphalan, chlorambucil, methlorethamine, TESPA, etc.; steroids, such as betamethasone, prednisolone, etc., and other miscellaneous agents, such as vinblastine, vincristine, asparaginase, mitotane, cisplatin, etc. When using combinations of two or more neoplastic agents, the dosage of m-AMSA salt may be reduced. The dosage ranges generally employed for the above cited antineoplastics which may be used with m-AMSA are known to the medical profession, see Physicians' Desk Reference, 34th Edition, Medical Economics Co. 1980, which is incorporated by reference. The invention is illustrated by the following examples. EXAMPLE t m-AMSA D-Gluconate A solution of 3.93 gm of m-AMSA and 5.15 gm of D-gluconic acid-water solution (38% w/w) in 305 ml of specially denatured 3A anhydrous ethanol at 70-80-C is filtered with suction. The filtrate is cooled to 30--35 C and 100 ml of anhydrous diethyl ether is added with stirring. After chilling to 0-5 C with stirring, the mixture is stored about 16 hours at 5 C. The product is collected, washed with specially denatured 3A anhydrous ethanol and anhydrous diethyl ether (3:1) at C, then with several portions of anhydrous ether and dried under reduced pressure at 50-55"C to give 3.95 gm of m-AMSA D-gluconate. The salt obtained by this process retains a small amount (about 1% of ethanol). EXAMPLE 2 m-AMSA DGalacturonate A solution of .3.93 gm of m-AMSA and 2.12 gm.of Dgalacturonic acid monohydrate in 325 ml of specially denatured 3A anhydrous. ethanol at 70-80"C is filtered with suction. The filtrate is stirred and chilled to 0-5 C, then stored at -20 C for about 16 hours. The product is collected, washed with cold (about -20 C) specially denatured 3A anhydrous ethanol, then with anhydrous diethyl ether and dried under reduced pressure at 50-55 C to give 3.9 gm of m-AMSA D-galacturonate. The salt obtained by this process retains a-small amount about 38 of ethanol. EXAMPLE 3 m-AMSA D-Glucose-6-phosphate A mixture of 1.1804 gm of m-AMSA and 0.8576 gm of (91%) D-glucose-6-phosphoric acid monohydrate in 19.3 ml of dimethylformamide is heated on a hot plate until a complete solution is obtained at about 1000C. The hot, dark-colored solution is then diluted with an equal volume of warm (65-70 C)-specially denatured 3A anhydrous ethanol and filtered immediately. Additional warm specially denatured 3A anhydrous ethanol (about 70-C) is used to rinse the funnel so that a total of 96.5 ml of ethanol is used. The mixture is allowed to cool to about 20-25'C with Occasional swirling' of the flask. The mixture is then codled for several hours at 3-5 C and then for a.similar period of time at -18 to -20 C. The solid is collected,' washed thoroughly with cold (3-5 C) specially denatured 3A anhydrous ethanol, then with anhydrous diethyl ether and dried under reduced pressure. at 39-40 C to give 1.38 gm of m-ARS Dglucose-6-phosphate. EXAMPLE 4 m-AMSA D-Glucuronate 'A A solution of 3.93 gm of m-AMSA and 2.0. gm of D-glucuronic acid in 325 ml specially denatured anhydrous ethanol at 70 -80 C is filtered with suction. After cooling to 30-35'C, 45 ml anhydrous diethyl ether is added with stirring and chilling to 5-C After chilling about 16 hours at 5 C, the salt is collected, washed with cold (0-5 C) specially'denatured 3A anhydrous ethanol1 then with anhydrous diethyl ether and dried under reduced pressure at 500C to give 4.5 gm of m-AMSA D-glucuronate. EXAMPLE 5 m-AMSA Gluconate Lyophilized Injectable Formulation per liter 1. m-AMSA Gluconate (11.77 mg/ml*) 11.77 g 2. Gluconic Acid, 50% Solution g.s. 3. Celite 521** 1.5 g 4. Water for Injection, USP q.s. to make 1000 ml *Equivalent to 7.5 mg/ml of m-AMSA plus 3% intentional excess. **If needed to clarify solution. Method of Preparation (for 1000 ml): A. Add sufficient 2 to approximately 900 ml of4 in a suitable container to adjust the pH of the solution to approximately 2.2. B. Add 1 slowly with continuous stirring until 1 completely dissolves. C. Recheck pH and adjust to 2.3-2.7, if needed, with 2. D. Add a sufficient amount of 4 to make 1000-ml of solution and mix well. E.. If necessary, clarify solution with 3 followed by suitable filtration to remove 3 (Whatman No. 1 filter paper, Millipore AW 19 membrane or equivalent). F. Sterilize solution by filtration through a pre viously sterilized membrane (Millipore GS or equivalent) using appropriate prefiltration, if necessary. G. Aseptically fill into previously sterilized vials (10 ml per vial). H. Stopper vials loosely with rubber lyophilization stoppers and lyophilize in a suitable lyophilizer, (75 mg m-AMSA per vial). I. At the conclusion of the lyophilization cycle, stopper and cap vials. EXAMPLE 6 m-AMSA Gluconate Injectable Formulation per 1000 vials. m-AMSA gluconate (117.7 mg/vial*) 117.7 g *Equivalent to 75 mg of m-AMSA + 3% intentional excess. Method of Preparation: 1. Presterilize the m-AMSA gluconate. 2. Fill into appropriate** (10 ml-20.ml) previously. sterilized vials. 3. Stopper and cap vials. **If 10 ml vials are used and 10.0 ml of Sterile Water for Injection is used to dissolve the sterile powder, the resulting concentration will be equivalent to 7.5 .mg/ml, m-AMSA, as the gluconate. If 20 ml vials are used and 20.0 ml of Sterile Water for Injection is used to dissolve the sterile powder, the resulting concentration will be 3.75 mg/ml. EXAMPLE 7 m-AMSA Gluconate Oral Formulation mg/cap g/1000 caps 1. m-AMSA Gluconate 76.1* mg 76.2 g 2, Lactose USP Hydrous 438.8 438.8 3. Polysorbate 80 USP 5.0 5.0 4. Syloid 74 (silica gel) 10.0 10.0 5. Alcohol SD 3A Anhydrous 0.07 ml ¯ 7.0 ml 530 mg '530 *Equivalent to 50 mg m-AMSA Base. Method of Preparation: A. Dissolve 3 in 5 and add to 4 contained in a suitable blender. Blend thoroughly. Transfer the wet mass onto trays and dry at about 120'F overnight. Add to 2 and mix well. Bo Screen 1 through a No. 60 screen. Add material from Step A and blend -thoroughly. Pass through No. 60 screen and reblend; C. Fill 530 mg of the powder mixture into No. "0" dark brown opaque hard gelatin capsules.

Claims: CLAIMS (for all states other than Austria): 1. A compound having the following general formula: EMI19.1 wherein X is an acid having a total of from three to six carbon atoms, two to five hydroxy groups and an acidic group selected from -CO2H and -OPO3H2 with the proviso that, when the total number of carbon atoms in the acid is three or four, the acidic group is -OPO3H2. 2. The compound of claim 1 having the name 4'-(9-acridinylamino)methanesulfon-m-anisidide Dgluconate. 3. The compound of claim 1 having the name 4' -(9-acridinylamino)methanesulfon-m-anisidide D-glucuronate. 4. The compound of claim 1 having the name 4'-(9-acridinylamino)methanesulfon-m-anisidide D-galacturonate. 5. The compound of claim 1 having the name 4'-(9-acridinylamino)methanesulfon-m-anisidide B-glycerophosphate. 6. A pharmaceutical composition comprising a compound according to claim l and a pharmaceutically acceptable carrier therefor. 7. A pharmaceutical composition according to claim 6, which also includes an excess of an acid of formula X' which is selected from the same class of acids as acid X. 8. A pharmaceutical composition according to claim 6 or 7, in which the composition is sterile, lyophilized and contained in a hermetically sealed container. 9. A pharmaceutical composition according to claim 6, 7 or 8, which also includes another anti-neoplastic agent. 10. A process for producing a compound according to claim 1, which process comprises reacting a compound having the following formula: EMI20.1 with an acid X wherein X is as defined in claim 1. CLAIMS (for Austria alone): 1. A process for producing a compound having the following general formula: EMI21.1 wherein X is an acid having a total of from three to six carbon atans, two to five hydroxy groups and an acidic group selected from -CO2H and -OPO H with the proviso that, when the total number of carbon atoms is three or four, the acidic group is -OPO3H2; which process comprises reacting a compound of the following formula: - EMI21.2 with an acid X wherein X is as defined above. 2. A process according to claim 1, wherein the acid X is D-gluconic acid. 3. A process according to claim 1, wherein the acid X is D-glucuronic acid. 4. A process according to claim 1, wherein the acid X is D-galacturonic acid. 5. A process according to claim 1, wherein the acid X is B-glycerophosphoric acid. 6. A process for producing a pharmaceutical composition which comprises admixing a compound having the formula I in claim 1 with a pharmaceutically-acceptable carrier. 7. A process according to claim 6, which also includes sterilizing and lyophilizing the pharmaceutical composition. 8. A process according to claim 6 or 7, which also includes sealing the pharmaceutical composition in a hermetically sealed container. 9. A process according to claim 6, 7 or 8, which includes incorporating in the pharmaceutical composition an excess of an acid of formula X' which is selected from the same class of acids as acid X. 10. A process according to any one of claims 6 to 9, which also includes incorporating in the pharmaceutical composition another anti-neoplastic agent.

Inventors: FISHER, JAMES ROYAL, KULIER, CHARLES PETER
Applicants: WARNER-LAMBERT COMPANY
Application year: 1981
Main classification: C07D219
IPC codes: A61P35, A61K9, A61P31, A61K31, C07H7, C07C59, C07D219, C07F9