4-0-SUBSTITUTED -2-DEOXYSTREPTAMINE AMINOGLYCOSIDE DERIVATIVES, THEIR PREPARATION AND FORMULATIONS CONTAINING THEM

Abstract: Novel compounds are disclosed which are 2′-N-acylated or 2′-N-alkylated derivatives of a 4-O-substituted-2-­ deoxystreptamine aminoglycoside selected from the group consisting of: apramycin, α or β-(C₁ to C₆ alkyl)aprosaminide, α or β-(C₂ to C₆ hydroxyalkyl)aprosaminide, α or β-(C₂ to C₆ aminoalkyl)aprosaminide, α or β-(C₂ to C₆ protected aminoalkyl)aprosaminide, α or β-(C₃ to C₆ aminohydroxyalkyl)aprosaminide, α or β-(C₃ to C₆ (protected amino)hydroxyalkyl)aprosaminide, or a pharmaceutically-acceptable acid addition salt thereof; wherein the acyl or alkyl substituent group is selected from the group consisting of γ-amino-α-hydroxybutyryl, γ-protected amino-α-­ hydroxybutyryl, β-amino-α-hydroxypropionyl, β-­ protected amino-α-hydroxypropionyl, glycyl, N-­ protected glycyl, 3-hydroxypyrrolidine-3-carbonyl, N-­ protected 3-hydroxypyrrolidine-3-carbonyl, 3-­ hydroxyazetidine-3-carbonyl, N-protected 3-­ hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-­ yl)acetyl, N-protected 2-hydroxy-2-(azetidin-3-yl)acetyl, 4-amino-2-hydroxybulyl, 3-amino-2-hydroxypropyl, 2-­ aminoethyl, 3-hydroxypyrrolidine-3-methylene, 3-­ hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-­ yl)ethyl, C₂ to C₄ alkyl, C₃ to C₅ allyl, benzyl and substituted benzyl. They are useful for treating warm blooded animals suffering from bacterial diseases. They and 2′-N-acylated and 2′-N-alkylated derivatives of lividamine, paromamine and the 6′-N-protected derivatives of nebramine, neamine, gentamine C₁, gentamine C 1a , gentamine C₂, gentamine C 2a , and gentamine C 2b , and pharmaceutically-acceptable acid addition salts thereof can be prepared by reacting the corresponding aminoglycoside substrate with a nickel salt Ni (X₂), X being C₁ to C₁₀ carboxylate, in water, diluting with a polar organic solvent, treating with an alkylating agent or acylating agent, optionally, deprotecting the amino or N-­ protected substituents, optionally, reducing carbonyl por­ tions of an acyl group, and recovering as a free base or as a pharmaceutically-acceptable acid addition salt.

Description: 4-0-SUBSTITUTED-2-DEOXYSTREPTAMINE AMINOGLYCOSID DERIVATIVES, THEIR PREPARATION AND FORMULATIONS CONTAINING THEM. The invention relates to novel 2'-N-acylated and 2'-N-alkylated derivatives of 4-O-substituted-2deoxystreptamine amino-glycosides having antibacterial activity against a wide range of gram positive and gram negative bacteria. They are useful in pharmaceutical, veterinary and surface disinfectant formulations. These and related 2'-N-acylated and 2'-N-alkylated derivatives of related aminoglycosides are prepared with the help of a nickel salt. Examples of 4-O-substituted 2-deoxystreptamine containing aminoglycosides used in the process of the present invention include the antibiotics apramycin (see U.S. Patent Nos. 3,691,279, 3,853,709 and 3,876,767): EMI1.1 and nebramine: EMI2.1 The nomenclature of the above formula of apramycin is simplified by the following numbering system in which the carbons of the 2-deoxystreptamine moiety are numbered 1 through 6, the carbons of the octose moiety are numbered with a single prime, 1'-8', and the carbons of the hexose moiety are numbered with a double prime, l''-6''. EMI3.1 Analogously, the aprosaminide aminoglycosides referred to in this invention have the same numbering system as apramycin with respect to the octose and 2-deoxystreptamine moieties. EMI4.1 The numbering system for the pseudodisaccharides referred to in this invention, such as nebramine, has the carbons of the 2-deoxystreptamine moiety numbered 1-6 (the same as for apramycin and the aprosaminides), but the hexose carbons are numbered with a single prime, 1'-6'. EMI4.2 The prior art has not disclosed a direct acylation of the 2'-amino group of 2'-aminoglycoside antibiotics. According to the present invention there is provided a 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, a or 5-(C1 to C6 alkyl)aprosaminide, a or 5-(C2 to C6 hydroxyalkyl)aprosaminide, a or D-(C2 to C6 aminoalkyl)aprosaminide, a or 5-(C2 to C6 protected aminoalkyl)apros aminide, &alpha; B- (C3 to C6 aminohydroxyalkyl) aprosaminide, a or -(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmaceutically-acceptable acid addition salt therebf; wherein the acyl or alkyl substituent group is selected from the group consisting of y-amino-a-hydroxybutyryl, y-protected amino a-hydroxybutyryl, D-amino-a-hydroxypropionyl, D-protected amino-a-hydroxypropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3 carbonyl, N-protected 3-hydroxypyrrolidine 3-carbonyl, 3-hydroxyazetiine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl)acetyl, N-pro tected 2-hydroxy-2-(azetidin-3-yl) acetyl, 4-amino-2-hydroxybutyl, 3-amino-2-hydroxy- propyl, 2-aminoethyl, 3-hydroxypyrrolidine 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl)ethyl, C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl. This invention also provides formulations, pharmaceutical, veterinary, and adapted as a surface disinfectant, which comprise as an active ingredient a 2'-N-acylated or 2'-N-alkylated derivative of a 4-0substituted-2-deoxystreptamine aminoglycoside as above described excepting compounds bearing protected amino or N-protected substituents associated with at least one carrier or diluent therefor, as appropriate to the formulation. This invention also provides a process for preparing a 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as before described or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: : apramycin, CL or 5-(C1 to C6 alkyl)aprosaminide, CL or D- (C2 to C6 hydroxyalkyl)aprosaminide, CL or to to C6 protected aminoalkyl)aprosaminide, a or D-(C3 to C6 (protected amino)hydroxyalkyl) aprosaminide, lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2 gentamine C2a, and gentamine C2; with two to six molar equivalents of a nickel salt of the formula: : Ni(X)2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then car rying out the reaction at a temperature between OOC. and 40"C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used being a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, ss-protected aminoa-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used being C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide; and (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. This invention also provides a 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as before described for use in treating warm-blooded animals having bacterial infections or for use as a surface disinfectant. The process of this invention involves regioselective acylation or alkylation of the 2'-amino group of 4-O-substituted-2-deoxystreptam.ine aminoglycoside substrates. The instant process is carried out by adding an acylating agent or alkylating agent to a mixture of the aminoglycoside substrate and a C1 to C10 carboxylate salt of nickel (II) in an aqueous polar organic solvent, and stirring the mixture for the appropriate time at a temperature between OOC and 400C. The aminoglycoside substrates that are used in the process of this invention are selected from a group consisting of apramycin, a or D-(C1 to C6 alkyl) aprosaminide, a or D-(C2 to C6 hydroxyalkyl)aprosaminide, a or 5-(C2 to C6 protected aminoalkyl)aprosaminide, and a or R-(C3 to C6 (protected amino)hydroxyalkyl)aprosaminide, lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a and gentamine C 2b The acylating agents for use in the present invention are reactive derivatives of acyl moieties selected from the group consisting of yprotected amino-a-hydroxybutyryl, B-protected amino -hydroxypropionyl, N-protected glycyl, N-protected 3hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl)acetyl. The alkylating agents used in the instant process are C2 toC4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. The compounds claimed in the present invention include the above-mentioned apramycin and the various a or D-aprosaminide compounds substituted on the 2'-amino group by the above-listed acyl or alkyl moieties. As isolated from the process of this invention, these acylated compounds have their 2'-N substituent in the protected amino or N-protected form. These derivatives in the protected amino and N-protected derivatives are intermediates to their respective deprotected amino and N-deprotected antibiotic compounds; which antibiotic compounds are also claimed in the instant application. Additionally, the deprotected amino acyl derivatives may undergo reduction of the carbonyl group to give a methylene group. These deprotected amino acyl derivative compounds and the N-deprotected compounds and all of the 2'-N alkyl derivatives, either in their free base form or their pharmaceutically acceptable acid addition salt form, are antibacterial agents and are also claimed compounds in the instant invention. This invention is directed to the 2'-Nacylated and 2'-N-alkylated derivatives of apramycin, a or D-(C1 to C6 alkyl)aprosaminide, a or ss-(C2 to C6 hydroxyalkyl)aprosaminide, a or 5-(C2 to C6 aminoalkyl)aprosaminide, Q or 5-(C2 to C6 protected aminoalkyl)aprosaminide, a or ss-(C3 to C6 aminohydroxyalkyl)aprosaminide, CL or ss-(C3 to C6 (protected amino)hydroxyalkyl)aprosaminide, and the pharmaceutically acceptable acid addition salts of these compounds. The acyl substituent on the 2'-amino group of the above compounds is selected from a group consisting of y-amino-ahydroxybutyryl, y-protected amino-a-hydroxybutyryl, B-amino-a-hydroxypropionyl, D-protected amino-a-hydroxy- propionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxypyrrolidine3-carbonyl, 3-hydroxyazetidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3yl)acetyl and N-protected 2-hydroxy-2- (azetidin-3-yl) - acetyl. Alkyl substituents on the 21-amino group of the above compounds are selected from the group consisting of the reduced derivatives of the above deprotectedamino acyl substituents and N-deprotected acyl substituents, namely: 4-amino-2-hydroxybutyl, 3-amino-2-hydroxypropyl, 2-aminoethyl, 3-hydroxypyrrolidine-3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin3-yl)ethyl, in addition to the alkyl substituents C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl. In the above description of the invention, the term "C1 to C6 alkyl" means a branched or straight chain alkyl such as methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, sec-butyl, t-butyl, n-pentyl, -methyl- pentyl, n-hexyl and like groups. Similarly, by the term "C2 to C6 hydroxyalkyl", we mean from one to three hydroxyl groups bonded to any carbon atom of a C2 to C6 alkyl chain, provided that no more than one oxygen atom is bonded to any single carbon atom of the alkyl chain. Examples of the hydroxyalkyl chains include: 2-hydroxyethyl, 2-hydroxy-n-propyl, 3-hydroxy-n-propyl, 1,3-dihydroxy-2-propyl, 2,4dihydroxy-n-butyl, 3-hydroxy-2-methylpropyl, 2,4,5trihydroxy-n-pentyl, 2, 4-dihydroxy-n-pentyl, 2-hydroxy4-methylpentyl, 5-hydroxy-n-hexyl, 4,5,6-trihydroxy-nhexyl, and like groups. By the term "C2 to C6 aminoalkyl", we mean from one to three amino groups bonded to any carbon atom of a C2 to C6 alkyl chain, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these aminoalkyl chains include: 2-aminoethyl, 2-amino-n-propyl, 3-amino-npropyl, 1,3-diamino-2-propyl, 2, 4-diamino-n-butyl, 3-amino-2-methylpropyl, 2,4,5-triamino-n-pentyl, 3amino-n-pentyl, 2-amino-4-methylpentyl, 5,6-diamino-n hexyl, 3-amino-n-hexyl, and like groups. By the term "C2 to C6 protected aminoalkyl", we mean from one to three protected amino groups bonded to any carbon atom of a C2 to C6 alkyl chain, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these protected aminoalkyl chains include the example compounds listed above for the C2 to C6 aminoalkyl chains wherein each of the amino groups are substituted by N-protecting groups, and like compounds. By the term "C3 to C6 aminohydroxyalkyl", we mean a C3 to C6 alkyl chain substituted at any carbon atom by one amino group and one hydroxyl group, or by two amino groups and one hydroxyl group, or by one amino group and two hydroxyl groups, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these aminohydroxyalkyl chains include: 2-hydroxy-3-amino-n-propyl, l-hydroxy-3-amino-2-propyl, 2-hydroxy-4-amino-n-butyl, l-hydroxy-4-amino-2-butyl, 2-hydroxy-5-amino-n-pentyl, 4,5-diamino-3-hydroxy-n-pentyl, 3,5-dihydroxy-4-aminon-pentyl, 2-amino-3-hydroxy-4-methylpentyl, 2-hydroxy6-amino-n-hexyl and like groups. By the term "C3 to C6 (protected amino)hydroxyalkyl", we mean a C3 to C6 alkyl chain substituted at any carbon atom by one protected amino group and one hydroxyl group, or by two protected amino groups and one hydroxyl group, or by one protected amino group and two hydroxyl groups, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these (protected amino)hydroxyalkyl chains include the examples listed above for the C3 to C6 aminohydroxyalkyl chains wherein each of the amino groups are substituted by N-protecting groups, and like compounds. The terms "protected amino" and "N-protected" used in the above description refer to an amino group substituted with one of the commonly employed amino blocking groups that are compatible with the present reaction conditions. Representative suitable aminoblocking groups for the purpose of this invention are described by J.W. Barton in "Protective Groups in Organic Chemistry," J.F.W. McOmie, Ed., Plenum Press, New York, N.Y., 1973, Chapter 2 and T. W. Greene "Protective Groups in Organic Synthesis," John Wiley and Sons, New York, N.Y., 1981, Chapter 7. Preferred amino blocking groups are the various alkoxycarbonyl and aryloxycarbonyl groups, with the most preferred amino blocking group being benzyloxycarbonyl. By the term "pharmaceutically acceptable acid addition salts" we mean those formed by standard acidbase reactions between the appropriate aminoglycoside and organic or inorganic acids. Such acids include hydrochloric, sulfuric, phosphoric, acetic, succinic, citric, lactic, maleic, furmaric, palmitic, cholic, pamoic, mucic, D-glutamic, d-camphoric, glutaric, glycolic, phthalic, tartaric, lauric, stearic, salicylic, methanesulfonic, benzenesulfonic, sorbic, picric, benzoic, cinnamic and like acids. By the term l'C2 to C4 alkyl", we mean ethyl, n-propyl and n-butyl. By the term "C3 to C5 allyl" we mean allyl, 2-methylallyl, 3,3-dimethylallyl, 3-methylallyl, and 2,3 dimethylallyl. Those skilled in the art will recognize that the latter two allyl substituents can exist in either the E or Z geometric isomers. In the present invention, it is understood that the allyl compounds can exist in the pure E or Z form, or as a mixture the two isomers. By the term "substituted benzyl" we mean a benzyl moiety where the phenyl ring is substituted with one substituent selected from the group consisting of fluoro, chloro, bromo, iodo, methyl, methoxy and trifluoromethyl. This substituent can be in either the ortho, meta or para positions. Examples of these substituted benzyl groups include para-chlorobenzyl, para-bromobenzyl, para-iodobenzyl, para-methylbenzyl, para-methoxybenzyl, para-trifluoromethylbenzyl, metafluorobenzyl, meta-bromobenzyl, meta-methylbenzyl, meta-methoxybenzyl, meta-chlorobenzyl, ortho-chlorobenzyl, ortho-iodobenzyl, ortho-methylbenzyl, orthomethoxybenzyl, ortho-bromobenzyl, and the like. Those skilled in the art will recognize that all of the above listed acyl substituents except glycyl and N-protected glycyl, the corresponding reduced acyl substituents, namely: 4-amino-2-hydroxybutyl, 3-amino2-hydroxypropyl, 3-hydroxypyrrolidine-3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin3-yl)ethyl, as well as many of the substituents described by the terms "C1 to C6 alkyl", "C2 to C6 hydroxyalkyl", "C2 to C6 aminoalkyl", "C2 to C6 protected aminoalkyl", "C3 to C6 aminohydroxyalkyl" and "C3 to C6 (protected amino)hydroxyalkyl" can exist as optical isomers. These terms refer to either the individual R or S isomers or to equal or unequal mixtures of the R and S isomers at each of the asymmetric carbon atoms for the purposes of this invention. The process of making the above 2'-N-acylated and 2'-N-alkylated compounds is the other aspect of this invention. Specifically, this process substantially selectively acylates or alkylates the 2-amino group of the following substrates: apramycin, &alpha; or ss-(C1 to C6 alkyl) aprosaminide, a or ss-(C2 to C6 hydroxyalkyl)aprosaminide, a or -(C2 to C6 protected aminoalkyl)aprosaminide, a or to to C6 (protected amino)hydroxyalkyl)aprosaminide, lividamine, paromamine, and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2b, by (1) combining the above aminoglycoside with between two and six molar equivalents of a nickel salt of the formula: : Ni (X)2 in water; (2) diluting the above mixture with between about two and ten volumes of a polar organic solvent; and (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above dilution of step (2) then carrying out the reaction at a temperature between OOC. and 40 C. for a period between 0.5 and 24 hours for acylation, and between 15 hours and 60 hours for alkylation. The terms ''C1 to C6 alkyl", "C2 to C6 hydroxyalkyl", "C2 to C6 protected aminoalkyl", "C3 to C6 (protected amino)hydroxyalkyl", "protected amino", and "...N-protected..." are as defined above for the compounds of the instant invention. Three of the above-listed aminoglycoside substrates for the process of this invention, namely apramycin, lividamine and paromamine, are known compounds and therefore their synthesis or isolation can be found in the literature. The pseudodisaccharides nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C 2b are also known compounds and therefore their synthesis or isolation can also be found in the literature. These pseudodisaccharides can be converted to their 6'-N-protected derivatives which are required as substrates for the instant process by methods analogous to the procedure given in Example 4, Part A of the Experimental Section. The various substituted a or ss aprosaminide substrates listed above can be made by methods analogous to the procedure described by S. O'Connor, et al., Journal of Organic Chemistry, 41, 2087 (1976). The acylating agents used in the instant process are also known in the art. Additional examples for converting the known carboxylic acid precursors into suitable acylating agents for the process of this invention are given in Preparations 1 and 2 in the Experimental Section. As with the compounds claimed in the present invention, it will be recognized by those skilled in the art that certain of the acylating agents, except for the reactive derivative of N-protected glycyl, certain C1 to C6 alkyl chains and certain substituted C2 to C alkyl chains and C3 to C6 alkyl chains of the a or ss substituted aprosaminide substrates can exist as optical isomers. For the purposes of the process of this invention, these moieties can be used as their pure R or S configurations or as mixtures of equal or unequal proportions of these two configurations at each of the asymmetric carbon atoms. In the above description of the process of the instant invention, the formula: Ni(X)2 refers to a nickel salt wherein the nickel is in the formal (II) oxidation state, and X refers to a C1 to C10 carboxylate ligand. The term "C1 to C10 carboxylate" includes straight or branched chain aliphatic carboxylates, as well as aromatic carboxylates, with acetate being the preferred carboxylate. Exemplary nickel salts used in the instant process include: Ni (acetate) 2 Ni (propionate) 2 Ni (hexanoate) 2 Ni(2-ethylhexanoate)2 Ni (benzoate) 2 Ni (octanoate) 2 Ni(neodecanoate)2. Although not explicitly depicted in the above formula and examples, the nickel salts used in the process of this invention may be either anhydrous or hydrated. The term "acylating agent", as used in the above description of the instant process, means a reactive derivative of a carboxylic acid which is capable in the instant process of coupling the acid to an amino group by an amide linkage. The carboxylic acids that are converted into reactive derivatives for use as acylating agents in the process of this invention are selected from a group consisting of y-protected amino-a-hydroxybutyric acid, 8-protected amino-a-hydroxypropionic acid, N-protected glycine, N-protected 3-hydroxypyrrolidine-3-carboxylic acid, N-protected 3-hydroxyazetidine-3-carboxylic acid, and N-protected 2-hydroxy-2-(azetidin-3-yl)acetic acid. Examples of reactive derivatives of a carboxyl group are an acid halide, an acid anhydride, an activated ester, an acid azide and the like. Preferred reactive derivatives are acid anhydrides and activated esters, with the more preferred reactive derivatives being acid anhydrides and the activated esters formed with Nhydroxysuccinimide and N-hydroxyphthalimide. After the 2'-(N-protected and amino-protected) acyl derivative compounds have been prepared by the instant process, they can be converted to their corresponding deprotected acyl derivatives by procedures well known in the art. Furthermore, the above deprotected amino or deprotected nitrogen acyl derivative compounds may have their carbonyl groups reduced to methylene groups with diborane or lithium aluminum hydride; these newly formed alkyl derivative compounds still retain biological activity. Methods for reducing the carbonyl groups are well known in the art, and include methods such as the ones described by K. Igarashi, et al. in U.S. Patent 4,201,774, herein incorporated by reference. An additional reference for the above reduction procedure is K. Richardson et al., J. Antibiotics, 30, 843 (1977). The alkylating agents used in the instant process are C2 to C4 alkyl bromide, C2 toC4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. The preferred alkylating agents are ethyl iodide, n-butyl bromide, n-butyl iodide, allyl bromide, allyl iodide, and benzyl bromide. It should be noted that the instant process will result in the 2',6'-di-N-acylation of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C when the 6'-amino C 2b group of these substrates is not protected. Therefore, in order to acylate the above pseudodisaccharide substrates on the 2'-amino group only, it is necessary to block the 6'-amino group with a suitable amino protecting group as defined above. This protecting group can then be removed after the 2'-amino group is substituted to yield the desired aminoglycoside derivative. An example of a 6'-N protection + 2'-N acylation + 6'-N deprotection procedure is given in Example 4 of the Experimental Section. The first step of the instant process involves forming the nickel salt:aminoglycoside complex. This step is accomplished by mixing the aminoglycoside substrate compound and the nickel salt in water. Thorough mixing of these two components in the water is crucial and can be accomplished by employing methods such as sonication, vigorous stirring and mixing, moderate warming, etc. An aqueous solution is usually obtained; if not, a thoroughly mixed or dispersed suspension is sufficient. Between about a 2:1 to about a 6:1 molar ratio of nickel salt:aminoglycoside substrate can be used, with the usual ratio employed being about 4:1. The second step of the instant process entails diluting the above mixture with a polar, organic solvent. By the term "polar, organic solvent" we mean a solvent such as formamide, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, hexamethylphosphoramide, t-butanol, tetrahydrofuran, acetonitrile, acetone, sulfolane, tetramethylurea, 2-pyrrolidinone, N-methylpyrrolidinone, dioxane, and the like, or a mixture of any of the above solvents, with the preferred solvent for acylation being dimethylformamide. The preferred "polar, organic solvent" for alkylation is also dimethylformamide. Between two and ten volumes of polar, organic solvent per volume of water can be used, with the usual amount employed being about 5 volumes of organic solvent per volume of water. The resultant mixture is usually stirred for about five minutes to about one hour before proceeding to the next step. The mixture can be either heterogeneous or homogeneous at this point. The final step of the instant process involves adding the appropriate acylating or alkylating agent to the mixture of step 2 of the process. The amount of acylating agent that can be used ranges from a 1:1 to a 2:1 ratio of acylating agent:aminoglycoside, with about a 1.5:1 ratio being typical. A ratio range of 5:1 to 20:1 of alkylating agent:aminoglycoside can be used, with the usual ratio employed being about 10:1. The time required to complete the acylation in the final step is not critical. The acylation reaction can be stirred for a period ranging between 0.5 and 24 hours, with the usual time being about 15 hours. The alkylation reaction requires longer periods of time, encompassing a range of fifteen hours to sixty hours. The temperature at which the instant process is carried out can range from between 0 C. to 400C. Typically the reaction is run between 200C. to 250C. Once the acylation reaction of the final step is completed, recognized isolation procedures may be utilized to obtain the desired purified product. One such procedure involves column chromatography on either silica gel or Chelex 100 resin (BioRad Labs, 2200 Wright Ave., Richmond, Ca. 94804) to adsorb the nickel salt from and separate the desired product from the resultant product mixture obtained from the instant process. To carry out this procedure, the solvent is evaporated in vacuo from the product mixture. The resultant residue is dissolved or suspended in an appropriate solvent and then loaded onto a column packed with the same solvent, using either Chelex 100 resin or silica gel as the stationary phase. An example of an appropriate solvent for use with the Chelex 100 resin is aqueous ammonium hydroxide. An example of an appropriate solvent for use with the silica gel is a 30:10:1 volume ratio of dichloromethane:methanol:ammonium hydroxide. The column is then eluted by common procedures (gradient or stepwise). The product can be chromatographed again on a second column of ion-exchange resin (e.g., Bio-Rex 70) or silica gel if necessary. Chelex 100 is a styrene divinylbenzene copolymer functionalized with iminodiacetate ions which act as chelating ligands for binding metal ions. Another isolation procedure removes Ni(II) ions by precipitation as the insoluble sulfide salts followed by ion-exchange chromatography to separate the desired products. Specifically, this procedure involves a) evaporating the solvent from the product mixture, dissolving the resultant residue in dilute acid (e.g., Q.1N HC1) and bubbling hydrogen sulfide gas through this mixture until precipitation of the nickel ion is complete, and b) chromatographing the filtered solution from step a) on an ion-exchange resin such as Bio-Rex 70 (BioRad Labs, supra) using an ammonium hydroxide solution or gradient to elute the desired product. Bio-Rex 70 is a weakly acidic cation exchange resin containing carboxylic acid groups on a macroreticular acrylic polymer lattice. Similar useful ion-exchange resins are Amberlite CG-50 and Amberlite IRC-50 (Rohm and Haas Co., Philadelphia, Pa.). The 2'-N-acylated and 2'-N-alkylated aminoglycosides made by the process of this invention, some of which having the 2'-N substitutent in the protected amino or N-protected form, and in addition some of the various pseudodisaccharides having the 6'-amino group protected, are intermediates to their respective deprotected amino and N-deprotected compounds. These 2' (protected amino and N-protected)acyl derivatives can be converted to their corresponding deprotected forms according to procedures well recognized in the art. These deprotected compounds, either in their free base form or their pharmaceutically acceptable acid addition salt form, are useful for treating infections in warm-blooded animals caused by gram-positive and gramnegative bacteria. These compounds can be administered parenterally, in the free base form or in the pharmaceutically acceptable acid addition salt form, using pharmaceutically acceptable formulations known in the art. These compounds can also be administered as veterinary compositions, such as, for example, in the feed or drinking water of farm animals to treat infections such as colibacillosis or swine disentery Alternatively, these compounds can be used as a surface disinfectant. Solutions containing as little as 0.01 percent by weight of the antibiotic are effective for disinfecting purposes. Such solutions, preferably also containing a detergent or other cleansing agent, are useful for disinfecting objects such as glassware, dental and surgical instruments, and surfaces such as walls, floors, and tables in areas where maintenance of sterile conditions is important, i.e. hospitals, food-preparation areas, and the like. The antibacterial activity of the deprotected compounds of this invention is illustrated by the following in vitro and in vivo test data obtained with representative compounds. In Table I, the minimum inhibitory concentration (MIC) for representative compounds against a wide range of gram-positive and gram-negative bacteria is presented. The MIC values were obtained by the standard agar dilution test. Table 1 Antibiotic Activity of 2'-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Staphylococcus aureus X1.1 16 16 16 4 32 Staphylococcus aureus V41 32 32 64 8 64 Staphylococcus aureus X400 64 64 128 16 64 Staphylococcus aureus S13E 16 16 32 8 32 Staphylococcus epidermidis EPI1 16 16 16 8 64 Staphylococcus epidermidis EPI2 4 8 8 2 32 Streptococcus pyogenes C203 32 32 64 16 128 Streptococcus pneumoniae Park I 32 32 128 16 128 Streptococcus group D X66 > 128 > 128 > 128 128 > 128 Streptococcus group D 9960 > 128 > 128 > 128 > 128 > 128 Hemophilus influenzae C.L. 64 32 16 16 64 Hemophilus influenzae 76 32 32 32 16 64 Shigella sonnei N9 32 32 16 8 64 Escherichia coli N10 16 16 64 8 128 Escherichia coli EC14 32 32 32 8 > 128 Table 1 cont. Antibiotic Activity of 2'-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Escherichia coli TEM 8 8 8 2 32 Klebsiella pneumoniae X26 8 8 8 4 64 Klebsiella pneumoniae KAE 16 32 16 8 64 Enterobacter aerogenes X68 16 16 16 8 64 Enterobacter aerogenes C32 16 16 16 8 64 Enterobacter aerogenes EB17 16 32 16 8 64 Enterobacter cloacae EB5 32 32 32 16 128 Enterobacter cloacae 265A 64 64 32 32 > 128 Salmonella heidelberg X514 32 32 32 16 128 Salmonella typhimurium 1335 16 32 64 8 128 Pseudomonas aeruginosa X528 128 128 128 64 > 128 Pseudomonas aeruginosa X239 64 128 64 64 128 Pseudomonas aeruginosa Psl8 128 128 128 32 128 Serratia marcescens x99 16 16 16 8 128 Serratia marcescens SE3 32 32 32 16 > 128 Table 1 cont. Antibiotic Activity of 2'-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Proteus morganii PR15 128 128 128 32 64 Proteus inconstans PR33 16 32 32 8 64 Proteus rettgeri PR7 8 16 32 8 32 Proteus rettgeri C24 16 16 16 8 64 Citrobacter freundii CF17 32 32 64 16 64 Bordetella bronchiseptica 16 128 128 128 32 > 128 #Numerals and letters following the names of test microorganisms refer to the strains. 1 Test compounds numbered 1-5 are as follows: 1 = 2'-N- (S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin 2 = 2'-N- (RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin (racemic side chain) 3 = 2'-N-glycylapramycin 4 = 2'-N-ethylapramycin 5 = 2'-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) nebramine Table II illustrates the effective dose (ED50, effective dose to protect 50 percent of the test animals) for representative compounds of this invention against an experimental bacterial infection in mice Table II Subcutaneous Therapy of Staphylococcus aureus 3055 Infections in Mice Test Compound Effective Dose (ED50) 2'-N-(S-y-amino-a-hydroxybutyryl)- 1 apramycin 6.5 2'-N-(RS-&gamma;-amino-&alpha; ;-hydroxybutyryl)- apramycin 21.8 2'-N-glycylapramycinsHC1 30 2'-N-ethylapramycin < 4.351 2'-N-(S-y-amino-a-hydroxybutyryl)- 3 nebramine > 70 * mg/kg x 2 subcutaneous doses at 1 and 5 hours post infection 1. mice were challenged with 68.1 LD50's. 2. mice were challenged with 331 LD50 's. 3. mice were challenged with 1,794 LD50's. 4. Mixture of diastereomers The ED50 values were determined as described by W.E. Wick et al., Journal of Bacteriology, 81 [No. 2], 233-235 (1961). The following Examples (1-8) are provided to further illustrate this invention. Preparations 1 and 2 provide examples of methods for synthesizing activated esters used in the process of the present invention. It is not intended that this invention be limited in scope by reason of any of the preparations or examples. In the following preparations and examples, carbon-13 nuclear magnetic resonance spectra, field desorption mass spectra and optical rotations are abbreviated c.m.r., f.d.m.s., and o.r., respectively. The nuclear magnetic resonance spectra were obtained on either a Varian Associates FT-80 Spectrometer or a JEOL JNM-PS-100 Spectrometer using dioxane as the internal standard. The chemical shifts are expressed in d values (parts per million downfield from tetramethylsilane. The field desorption mass spectra were taken on a Varian MAT 731 Spectrometer using carbon dendrite emitters. Optical rotations were measured on a Perkin Elmer Model 241 Polarimeter. Thin layer chromatography was carried out on E. Merck silica gel plates. Preparation 1 Preparation of N-(S-y-Benzyloxycarbonylamino-ahydroxybutyryloxy)succinimide 5-y-Benzyloxycarbonylamino-a-hydroxybutyric acid (10.1 g, 40 mmol) and N-hydroxysuccinimide (4.60 g, 40 mmol) were dissolved in ethyl acetate (220 ml) and cooled in an ice bath. N,N'-Dicyclohexylcarbodiimide (8.25 g, 40 mmol) was added and the reaction mixture was stirred overnight. The resulting white precipitate was filtered and the filtrate was evaporated to dryness under reduced pressure. The residue was triturated with pentane (200 ml). The resultant white solid was filtered, washed with pentane and air-dried to yield 9.37 g (67%) of product. Crystallization from ethyl acetate yielded purified material, m.p. 1080C. Preparation 2 Preparation of N- (N-Benzyloxycarbonylglycyloxy) succinimide N-Benzyloxycarbonylglycine (10.9 g, 52.2 mmol) and N-hydroxysuccinimide (6.0 g, 52.2 mmol), were dissolved in dioxane (100 ml) and cooled in a cold water bath. N,N'-Dicyclohexylcarbodiimide (10.8 g, 52.2 mmol) was added and the mixture was stirred overnight. The white precipitate which formed was filtered, washed and air-dried (11.8 g, 100% of dicyclohexylurea). The filtrate was evaporated to dryness under reduced pressure, and the residual solid (16.2 g) was crystallized from ethyl acetate (50 ml) to give 8.4 g (53%) of crystalline N- (N-benzyloxycarbonylglycyloxy) succinimide, m.p. 110.5-112 C. Example 1 Preparation of 2'-N-(S-y-Amino-a-hydroxybutyryl)apramycin Apramycin (1.08 g, 2.0 mmol) and nickel(II) acetate tetrahydrate (1.99 g, 8 mmol) were dissolved in water (20 ml) and this solution was diluted with dimethylformamide (100 ml). The resultant opaque, blue-green mixture was stirred for one hour at ambient temperature and then treated with N-(S-y-benzyloxy carbonylamino-a-hydroxybutyryloxy)succimide (1.05 g, 3 mmol); a homogeneous green solution formed after about thirty minutes. After stirring overnight at ambient temperature, the solvent was evaporated under reduced pressure. The resultant residue was dissolved in a 30:15:2.5 mixture of dichloromethane:methanol: concentrated ammonium hydroxide and was loaded onto a silica gel column (Grace 950, 200 g) prewashed with the same solvent mixture. The column was successively eluted with three different mixtures of dichloromethane: methanol: concentrated ammonium hydroxide (30:15:2.5, 6:6:1, 3:3:1). The fractions containing the desired product were combined, the solvent was evaporated and the resultant colorless glassy residue was dissolved in water and was lyophilized to give 2'-N-(S-y-benzyloxycarbonylamino-a-hydroxybutyryl)apramycin (0.60 g, 39% yield). The above 2 '-N- (S-y-benzyloxycarbonylamino- a-hydroxybutyryl)apramycin (550 mg) was dissolved in a solution of water (30 ml), dioxane (10 ml) and glacial acetic acid (1 ml). 5% palladium on carbon (135 mg) was added and the mixture was hydrogenated -at 45 p.s.i. for 24 hours at room temperature. The palladium catalyst was removed by filtration and the filtrate was evaporated to dryness. The residual glassy product was dissolved in water and loaded onto a column of Bio-Rex 70 (290 ml, ammonium hydroxide cycle, pH 10.0). This column was eluted with a linear gradient of 0.005N ammonium hydroxide (1 1) and 0.5N ammonium hydroxide (1 1) followed by continued elution with 0.5N ammonium hydroxide. The fractions containing the desired product were combined, concentrated and lyophilized to yield 2'-N-(S-y-amino-a-hydroxybutyryl)apramycin (383 mg, 84% yield): c.m.r. (D20-H2O, pH > 10) 6 29.7, 32.9, 33.2, 36.3, 37.7, 48.8, 50.4, 51.0, 53.1, 61.6, 62.2, 66.1, 67.5, 70.5, 71.2, 71.7, 73.4, 74.2, 76.6, 78.3, 87.4, 95.4, 96.5, 98.5, 175.9; (at pH < 2) 6 29.0, 29.2, 31.1, 31.7, 37.6, 47.9, 50.0, 50.6, 53.0, 60.4, 61.2, 63.7, 67.7, 69.0, 70.1, 70.3, 70.6, 71.2, 73.4, 76.1, 78.8, 93.7, 95.3, 97.5, 175.5; f.d.m.s. m/e 623 (P+ 1-18); thin layer chromatography, Rf = 0.14 using a 3:1:2 volume ratio of methanol:methylene chloride: concentrated ammonium hydroxide. Example 2 Preparation of 2'-N-(RS-y-Amino-a-hydroxybutyryl)- apramycin The same procedure was followed as described in Example 1, except the acylating agent employed was N-(RS-y-benzyloxycarbonylamino- -hydroxybutyryloxy)- succinimide (racemic mixture). The product obtained from this reaction, 2'-N-(RS-y-amino-a-hydroxybutyryl)- apramycin, had the following physical properties: c.m.r. (D2O-H2O, pH < 3) 6 29.3 (2x), 31.4, 31.9, 37.8, 48.0, 50.2, 51.0, 53.4, 60.6, 61.4, 64.0, 67.7, 69.2, 70.2, 70.3, 70.6, 71.3, 73.6, 76.2, (78.8 and 79.1 -doublet), 93.7, 95.3, 97.4, 175.6; f.d.m.s. m/e 641 250 (p+l); o.r. [CL] D = + 124,7 (H2O,c = 10 mg/ml). Example 3 Preparation of 2'-N-Glycylapramycin Apramycin (2.16 g, 4 mmol) and nickel(II) acetate tetrahydrate (4.0 g, 16 mmol) were dissolved in water (40 ml) and the resultant solution was diluted with dimethylformamide (200 ml). To this mixture was added N- (N-benzyloxycarbonylglycyloxy) succinimide (1.84 g, 6 mmol). After stirring overnight at ambient temperature, solvent was evaporated under reduced pressure. The residue was dissolved in 0.1N hydrochloric acid (250 ml) and a slow stream of gaseous hydrogen sulfide was bubbled into the mixture for one hour. After stirring overnight, the precipitate was filtered and the filtrate was evaporated to dryness under reduced pressure. The resultant residue was suspended in 1N sodium hydroxide (35 ml) and was loaded on a column of 300 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 10, pre-eluted with 0.01N ammonium hydroxide). The column was eluted first with 0.01N ammonium hydroxide (400 ml) and then with a linear gradient of 0.0in ammonium hydroxide (2 1) and 0.05N ammonium hydroxide (2 1). Fractions containing the desired product were located by thin layer chromatography, combined and lyophilized to yield 2'-N- (N-benzyloxycarbonylglycyl) - apramycin (1.35 g, 46% yield). The above 2'-N- (N-benzyloxycarbonylglycyl) - apramycin was dissolved in a mixture of ethanol (100 ml) water (10 ml) and 1N hydrochloric acid (10 ml). 5% palladium on charcoal (3 g) was added. This mixture was hydrogenated at 60 p.s.i. at ambient temperature overnight. The palladium catalyst was removed by filtration and the filtrate was placed in the refrigerator overnight. The desired crystalline 2'-N-glycylapramycin pentahydrochloride (1.01 g, 78% yield) precipitated from solution and was collected by filtration, washed with ethanol and air-dried: c.m.r. (D2O-H2O, pE > 10) 6 29.7, 32.8, 35.1, 44.2, 48.4, 50.3, 51.1, 53.1, 61.6, 62.1, 66.1, 67.4, 71.2, 71.7, 73.4, 74.2, 76.6, 77.4, 87.0, 95.4, 96.3, 98.5, 174.5; f.d.m.s. m/e 250 597 (P+1); o.r. [ ]D = + 127.3 (H2O, C = 8.3 mg/ml); thin layer chromatography, Rf = 0.32 using a 3:1:2 volume ratio of methanol:methylene chloride:concentratod ammonium hydroxide. Example 4 A. Preparation of 6'-N-Benzyloxycarbonylnebramine Nebramine (15.3 g, 50 mmol) was dissolved in water (400 ml) and tetrahydrofuran (THF) (150 ml). A solution of benzyloxycarbonylimidazole (11.6 g, 57 mmol) in THF (300 ml) was added dropwise over a 90 minute period and the reaction mixture was stirred overnight at ambient temperature. THF was evaporated under reduced pressure and the remaining aqueous solution was lyophilized. The resultant crude product was dissolved in water (100 ml) and loaded onto a column of 1500 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 9.7). The column was eluted stepwise with 0.005N ammonium hydroxide (1.5 1), 0.015N ammonium hydroxide (2 1), 0.03N ammonium hydroxide (5 1), and finally with 0.05N ammonium hydroxide (10 1). The fractions eluted with 0.05N ammonium hydroxide were analyzed by thin layer chromatography, and those containing the desired product were combined and lyophilized to give 6'-N-benzyloxycarbonylnebramine (9.7 g, 44%-yield). B. Preparation of 2'-N-(S-y-Amino-a-hydroxybutyryl)nebramine 6'-N-Benzyloxycarbonylnebramine (880 mg, 2 mmol) and nickel(II) acetate tetrahydrate (1.99 g, 8 mmol) were dissolved in water (20 ml) and this solution was diluted with dimethylformamide (100 ml). To the resultant mixture was added N-(S-y-benzyloxycarbonyl amino-a-hydroxybutyryloxy)succinimide (1.05 g, 3 mmol). After stirring overnight at room temperature, the solvent was evaporated under reduced pressure, the residue was dissolved in methanol and the solvent was again evaporated to remove traces of dimethylformamide. The resultant residue was dissolved in a 30:10:1 mixture of dichloromethane:methanol:ammonium hydroxide and was loaded on a silica gel column (Grace 950, 90 g) prewashed with the same solvent mixture. The desired product was eluted from the column using the 30:10:1 mixture of dichloromethane methanol : ammonium hydroxide as eluant. The fractions containing the desired product were combined and the solvent was evaporated. The residue was dissolved in a 1:1 mixture of water:dioxane, filtered and lyophilized to give 6'-N-benzyloxycarbonyl 2'-N-(S-y-benzyloxycarbonylamino- -hydroxybutyryl)- nebramine (1.06 g, 79% yield). 6' -N-Benzyloxycarbonyl-2 '-N- (S-y-benzyloxy- carbonylamino- -hydroxybutyryl)nebramine (975 mg) was dissolved in a mixture of dioxane (20 ml), water (20 ml) and glacial acetic acid (1 ml). 5% palladium on charcoal (270 mg) was added and the mixture was hydrogenated at 40 p.s.i. overnight at ambient temperature. The palladium catalyst was removed by filtration and the filtrate was concentrated under reduced pressure and then loaded onto a column of 200 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 8.6). After first eluting with a gradient of 0.005N ammonium hydroxide (2 1) and 0.4N ammonium hydroxide (3 1), the desired product was eluted with 0.5N ammonium hydroxide. The fractions containing the desired product were combined and lyophilized to yield 2'-N-(S-y-amino-a-hydroxybutyryl)nebramine (397 mg, 70% yield): c.m.r. (D20-H20, pH > 10) 6 32.4, 33.3, 36.2, 37.7, 42.0, 48.8, 50.4, 51.0, 66.4, 70.5, 73.6, 76.7, 78.4, 86.3, 97.3, 175.9; f.d.m.s. m/e 408 (P+1); thin layer chromatography, Rf = 0.23 using a 3:1:2 volume ratio of methanol:methylene chloride: concentrated ammonium hydroxide. Example 5 Preparation of 2'-N-Ethylapramycin Apramycin (1.08 g, 2.0 mmol) and nickel(II) acetate tetrahydrate (1.99 g, 8.0 mmol) were dissolved in water (20 ml) and the solution was diluted with dimethylformamide (100 ml). The resultant opaque blue-green mixture was stirred for one hour at ambient temperature and then ethyl iodide (3.12 g, 20 mmol) was added. After stirring overnight at ambient temperature, solvent was evaporated under reduced pressure. The residue was suspended in a 30:10:1 mixture of methanol: dichloromethane:concentrated ammonium hydroxide (40 ml) and was loaded onto a silica gel column (Grace 950, 150 g) prewashed with the same solvent mixture. The column was eluted stepwise with eight different mixtures of methanol:dichloromethane:concentrated ammonium hydroxide [(a) 30:10:1 (400 ml), (b) 30:10:3 (430 ml), (c) 30:10:5 (900 ml), (d) 30:10:6 (460 ml), (e) 30:10:7 (1 1.) (f) 30:10:8 (400 ml), (g) 30.10:10 (400 ml), (h) 30:10:15 (400 ml)]. The fractions containing the desired product were combined, concentrated and lyophilized, yielding 785 mg of crude product. This material was dissolved in water, the solution's pH was adjusted to 8.8 and then the solution was loaded on a column of 300 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 9.8, prewashed with 0.001N ammonium hydroxide (1 1)). The column was eluted with a linear gradient of 0.01N ammonium hydroxide (2 1.) and 0.10N ammonium hydroxide (2 1). Fractions containing the desired product were combined and lyophilized, yielding 2'-N-ethylapramycin (150 mg, 13% yield): c.m.r. (D20-H2O, pH > 10) 6 14.8, 30.8, 33.1, 36.8, 41.7, 50.4, 51.4, 53.5, 56.1, 61.8, 62.4, 66.3, 68.0, 71.4, 72.2, 73.8, 74.3, 76.9, 78.5, 88.5, 95.6, 96.4, 100.2; f.d.m.s. m/e 567 (P+); thin layer chromatography; Rf = 0.25 using a 6:2:3 volume ratio of methanol:methylene chloride: concentrated ammonium hydroxide. Example 6 Preparation of 2'-N-(n-Butyl)apramycin Method 1 Apramycin (54 mg, 0.1 mmol) was dissolved in water (1 ml). Nickel acetate tetrahydrate (100 mg, 0.4 mmol) was added and this solution was diluted with dimethylformamide (5 ml). The mixture was treated with l-iodo-n-butane (0.115 ml, 1 mmol3 and this reaction mixture was stirred at room temperature for 4 days. Thin layer chromatographic analysis (developed in 6:2:3 methanol:dichloromethane:ammonium hydroxide solution, visualized by chlorox spray and heating followed by starch-iodine spray reagent) of the reaction mixture showed 2'-N-(n-butyl)apramycin as the major product (Rf = 0.35) along with unreacted apramycin (Rf = 0.18). Bioautography of another thin layer chromatography plate against B. subtilis X12 showed a zone of inhibition due to 2'-N-(n-butyl)apramycin. Method 2 The reaction of Method 1 was repeated using l-bromo-(n-butane) (0.11 ml, 1 mmol) instead of 1iodo-(n-butane). This reaction appeared to give a lower yield of the desired 2'-N-(n-butyl)apramycin than did the reaction of Method 1. Example 7 Preparation of 2'-N-Benzylapramycin The reaction of Example 6, Method 1, was followed, except that benzyl bromide (0.06 ml, 0.5 mmol) was used instead of l-iodo-n-butane. The reaction mixture was stirred at room temperature for 19 h, at which time thin layer chromatographic analysis showed 2'-N-benzylapramycin (R5 = 0.32) to be the major product. Bioautography of a second thin layer chromatography plate against B. subtilis X12 showed a zone of inhibition due to 2'-N-benzylapramycin. Example 8 Preparation of 2' -N-Allylapramycin Method 1 The reaction of Example 6, Method 1, was followed, except that allyl bromide (0.043 ml. 0.5 mmol) was used instead of l-iodo-n-butane. The reaction mixture was stirred at room temperature for 22 h, at which time thin layer chromatographic analysis showed a substantial amount of 2'-N-allylapramycin (Rf = 0.27) was present along with an unidentified material (Rf = 0.38) and unreacted apramycin. Bioautography of a second thin layer chromatography plate against B. subtilis X12 showed a good zone of inhibition due to 2'-N-allylapramycin. Method 2 The reaction of Method 1 above was repeated, except that allyl iodide (0.05 ml, 0.55 mmol) was used instead of allyl bromide. The reaction mixture was stirred at room temperature for 22 h, when thin layer chromatographic analysis of the reaction mixture gave the same results as in Method 1.

Claims: Claims 1. A 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, a or ss-(Cl to C &alpha; or ss - (C2 to C6 hydroxyalkyl) aprosaminide, &alpha; or ss - (C2 to C6 aminoalkyl) aprosaminide, &alpha; or ss - (C2 to C6 protected aminoalkyl) apros aminide, &alpha; or ss - (C3 to C6 aminohydroxyalkyl) aprosaminide, a or ss-(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmacentically-acceptable acid addition salt thereof; wherein the acyl or alkyl substituent group is selected from the group consisting of &gamma;-amino-&alpha;-hydroxybutyryl, &gamma;-protected amino &alpha;-hydroxybutyryl, ss-amino-&alpha;-hydroxypropionyl, ss-protected amino-&alpha; ;-hydroxypropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3- carbonyl, N-protected 3-hydroxypyrrolidine 3-carbonyl, 3-roxyazetidine-3-carbonyl, N-orotected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl) acetyl, N-pro tected 2-hydroxy-2- (azetidin-3-yl) acetyl, 4 -amino-2-hydroxybutyl, 3-amlno-2-hydroxy- propyl, 2-aminoethyl, 3-hydroxypyrrolidine 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl) ethyl, C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted Denzyl. 2. A 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2-deoxystreptomine aminoglycoside as claimed in claim 1 selected from: 2'-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin, 2'-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin, 2' -N-glycylapramycin, 2' -N-ethylapramycin, 2'-N- (n-butyl) apramycin, 2 '-N-benzylapramycln, and 2'-N-allylapramycin. 3. A 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside as claimed in claim 1 selected from the group which includes the above compounds excluding those bearing protected amino or N-protected substituents. 4. A pharmaceutical formulation which comprises as an active ingredient a 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2deoxystreptamine aminoglycoside as claimed in claim 3 associated with at least one carrier or diluent therefor. 5. A veterinary formulation which comprises as an.active ingredient a 2'-N-acylated or 2'-Nalkylated derivated of a 4-O-substituted-2- eoxy- streptamine aminoglycoside as claimed in claim 3 asassociated with at least one carrier or diluent thereof. 6. A surface disinfectant formulation which comprises as an active ingredient a 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted-2- deoxystreptamine aminoglyceside as claimed in claim 3 associated with at least one carrier or diluent therefor. 7. A process for preparing a 2'-N-acylated or 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as defined in claim 1 or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine r and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: : apramycin, CL or D-(C1 to C6 alkyl) aprosaminide, a or ss- (C2 to C6 hydroxyalkyl)aprosaminide, a or to to C6 protected aminoaikyl)aprosaminide, CL or to C 6 or D-(C3 to C6 (protected amino)hydroxyalkyl)- aprosaminide, lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Clal gentamine C2 gentamine C2a, and gentamine C2; with two to six molar equivalents of a nickel salt of the formula: : Ni (X) 2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then car rying out the reaction at a temperature between OOC. and 400C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used being a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, B-protected aminoa-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used being C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide; and (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. 8. A process as claimed in claim 6 wherein the product is selected from: 2'-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin, 2'-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin, 2' -N-glycylapramycin, 2' -N-ethylapramycin, 2' -N- (n-butyl) apramycin, 2' -N-benzylapramycin, 2'-N-allylapramycin, and 2'-N-(S-y-amino- -hydroxybutyryl)nebramine. 9. A 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted 27deoxystreptamine aminoglycoside as defined in claim 3 for use in treating warm-blooded animals having bacterial infections. 10. A 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as defined in claim 3 for use as a surface disinfectant. 11. A 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as defined in claim 3, for use as a pharmaceutical Claims 1. A process for preparing a 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted-2deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, &alpha; or ss - (C1 to C6 alkyl) aprosaminide, &alpha; or ss - (C2 to C6 hydroxyalkyl) aprosaminide, a or ss-(C2 to C6 aminoalkyl)aprosaminide, a or ss-(C2 to C6 protected aminoalkyl)apros- aminide, CL or ss-(C3 to C6 aminohydroxyalkyl)- aprosaminide, &alpha; ; or ss- (C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmaceutically-acceptable acid addition salt thereof; wherein the acyl or alkyl substituent group is selected from the group consisting of y-amino-a-hydroxybutyryl, y-protected amino &alpha;-hydroxybutyryl, ss-amino-&alpha;-hydroxypropionyl, ss-protected amino-&alpha; ;-hydroxyropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3 carbonyl, N-prciected 3-hydroxypyrrolidine- 3-crbonyl, 3-hydroxyazetidine-3-carbonyl, N-orotected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl) acetyl, N-pro tected 2-hydroxy-2-(azetidin-3-yl) acetyl, 4-amine-2-hydroxybutyl, 3-amino-2-hydroxy propyl, 2-aminoethyl, 3-hydroxypyrrolidine- 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl)ethyl, C2 to C4 alkyl, C3 to C allyl, benzyl and substituted benzyl, or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine C1a gentamine C2, gentamine C2a, and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: apramycin, a or ss-(Cl to C6 alkyl)aprosaminide, a or ss- (C2 to C6 hydroxyalkyl)aprosaminide, a or to to C6 protected aminoalkyl)aprosaminide, a or ss-(C3 to C (protected amino)hydroxy 6 alkyl) aprosaminide, lividamine, paromamine and the 6'-N-protected derivatives of nebra mine, neamine, gentamine C1, gentamine Clal gentamine C2 gentamine C2a, and gentamine C2b; with two to six molar equivalents of a nickel salt of the formula: Ni(X)2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then carrying out the reaction at a temperature between OOC. and 400C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used being a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, 8-protected amino CL-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used being C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide; and (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. 2. A process of preparing 2'-N-(S-y-amino- a-hydroxybutyryl)apramycin as claimed in claim 1 which comprises reacting apramycin with nickel II acetate tetrahydrate in water, diluting the solution with di methylfowmamide, treating with N-(S-y-benzyloxy carbonylamino-a-hydroxybutyryloxy)succinimi e overnight to form 2'-N-(S-y-benzyloxvcarbonylamino-a-hydroxy- butyryl)apramycin, followed by removal of the protecting agent and recovering a free base. 3. A process of preparing a racemic mixture of 2'N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin as claimed in claim 1 which comprises reacting apramycin with nickel II acetate tetrahydrate in water, diluting the solution with dimethylformamide, treating with a racemic mixture of N-(S-y-benzyloxycarbonylamino-C-hydroxybutyryloxy)succinimide overnight to form a racemic mixture of 2'-N- (S-y-benzyloxycarbonylamino-CL-hydroxybutyryl) - apramycin, followed by removal of the protecting agent and recovering a free base. 4. A process of preparing 2'-N-glycyl apramycin as claimed in claim 1 which comprises reacting apramycin with nickel II acetate tetrahydrate in water, diluting the solution with dimethylformamide, treating with N- (N-benzyloxy carbonylglycyloxy)succinimide overnight to form 2'-N-(N-benzyloxycarbonylglycyl)- apramycin, followed by removal of the protecting agent and recovering as a free base. 5. A process of preparing 2'-N-(S-y-amino a-hydroxy-butyryl)nebramine as claimed in claim 1 which comprises reacting 6'-N-benzyloxycarbonyl nebramine and nickel II acetate tetrahydrate in water, diluting with dimethylformamide, treating with N-(S-y-benzyloxy carbonyl-amino-a-hydroxybutyryloxy)succinimide overnight to give 6' -N-benzyloxycarbonyl-2 '-N-S-y-benzyloxy- carbonylamino-a-hyd¯oxy butyryl) nebramine, followed by removal of the protecting agent and recovering as a free base. 6. A process of preparing 2'-N-ethylapramycin as claimed in claim 1 which comprises reacting apramycin and nickel II acetate tetrahydrate in water, diluting with dimethylformamide, and treating with ethyl iodide overnight and recovering the free base. 7. A process of preparing 2'-N-(n-butyl)apramycin as claimed in claim 1 which comprises reacting apramycin with nickel acetate tetrahydrate in water, diluting in dimethylformamide, treating with l-iodo-n-butane for 4 days, and recovering as the free base. 8. A process of preparing 2'-N-benzylapramycin as claimed in claim 1 which comprises reacting apramycin with nickel acetate tetrahydrate in water, diluting in dimethylformamide, treating with benzyl bromide for 19 hours, and recovering as a free base. 9. A process for the preparation of 2'-Nallylapramycin as claimed in claim 1 which comprises reacting apramycin with nickel acetate tetrahydrate in water, diluting with dimethylformamide, treating with allyl bromide for 22 hours, and recovering as a free base. 10. A 2'-N-acylated or a 2'-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as defined in claim 1 or any one of the group lividamine, paromamine and the 61-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2b or a pharmaceutically-acceptable acid addition salt thereof whenever prepared by the process as claimed in claim 1.

Inventors: KIRST, HERBERT ANDREW, TRUEDELL, BRENDA ALETHIA
Applicants: ELI LILLY AND COMPANY
Application year: 1981
Main classification: C07H15
IPC codes: A61K31, A01N63, C07H15, C07D207, A61P31