BIOLOGICAL AND CHEMICAL CONVERSION PROCESSES IN LIQUID PHASE-SYSTEM

Abstract: Method for carrying out biological and chemical conversion processes which require the presence of one or more catalytically active substances, such as enzymes, microorganisms, cells, organelles, cell homogenates, and organic or inorganic catalysts. The method is carried out in a liquid system consisting of at least two aqueous phases (F1, F2) so selected that the catalytic substance (K) is enriched in one (F1) of the phases, while the starting substrate (S) of the process is distributed in both phases or, advantageously, is enriched in the same phases as the catalytic substance, and the process product (P) is distributed in both phases or, advantageously, is enriched in the other phase (F2). During the process, the system is agitated to finely disperse one phase in the other. The process product can be recovered from the other phase, either by ceasing to agitate the system and allowing the phases to separate, or by maintaining dispersement of one phase in only a part of the other phase and continuously tapping off the upper part of the other phase for removal of the process product therefrom and then returning the removed phase part to the agitated part of the liquid system. Advantageously, the other phase (E2) of the liquid system has a substantially larger volume than the mentioned one phase (F1). The method can be applied with gaseous, low-molecular, macromolecular and particulate starting substrates, which may be soluble or insoluble in the system. The method according to the invention affords particular advantages when carrying out enzymatic conversion reactions requiring the presence of a coenzyme. In this case, the coenzyme and an enzyme operable to regenerate the coenzyme are also introduced to the system, the two phases of which are selected so that the coenzyme and the further enzyme are also enriched in the same phase as the enzyme requisite to the conversion reaction.

Description: BIOLOGICAL AND CHEMICAL CONVERSION PROCESSES INLIQUID PHASE -SYSTEM The present invention relates to a method of carrying out biological and chemical conversion processes which require the presence of at least one catalytically active substance, which may be, for example, enzymes, micro- organisms , whole cells , cell homogenates , organelles , or ' organic or inorganic catalysts. The realization of such conversion processes on a large scale is encumbered with several problems which have hitherto been found difficult to resolve in a satisfactory " manner. One such problem encountered is that of retaining the catalytic substance in the process chamber, so as to avo losing said substance, which in many cases is expensive, or obviate the need of providing complicated and expensive additional process steps for separating the catalytic substa from the process products removed from said process chamber. In certain- cases it is also desired to prevent the catalytic stance from being included in the desired final product of said process as an undesirable impurity. Attempts have been made to solve this problem by immobilizing the catalytic sub stance, by binding said substance to a carrier in some ' suita manner so that said substance can be held in the process cha and the aforementioned disadvantages eliminated, thereby enabling the process to be effected as a continuous process. This immobilisation of the catalytic substance, however, give rise to other problems. It has been found, for instance, that the activity of the immobilized catalytic substance is impair with respect to the starting substrate of the process, due to diffusion restrictions and steric hindrance, which- becomes particularly noticeable with macromolecular or particulate starting substrates. Thus, hitherto it has not been found possible to carry out biochemical conversioncprocesses with macromolecular or particulate starting substrates and while OM?I_ using immobilized catalytic substances, such as enzymes a microorganisms for example. One problem which is particularly difficult to res exists when carrying out biochemical conversion processes the use of a catalytically active enzyπr which is not able function unless in the presence of a coenzyme. In this ca is not only necessary for the enzyme and the coenzyme to retained in ~ he process chamber, but also for the coenzym be continuously regenerated over the duration of the proc so that it can be returned to its originally active confi tiσn with respect to the enzyme.- Hitherto, it has not bee possible to solve this problem in any way other than to u whole immobilized cells which contain both the enzyme req for the conversion process and the coenzyme, and in which generation of the coenzyme can also taJce place. Because o aforementioned reasons, however, such immobilized cells c be used in conjunction with macromolecular or particulate starting substrates ; and such cells also have the serious disadvantage whereby they result in a plurality of mutual different enzyme—activities in addition to the activity s fically desired for the conversion process, meaning that large number of undesirable secondary reactions can take place. A further serious problem often occurring in bioch conversion processes of the aforementioned kind, is that end product of the conversion process, or intermediate products formed in different stages in a conversion proce comprising a number of stages, often has a strong inhibit effect on the process or the various process stages. In c sequence hereof it is necessary to hold the concentration the end product and such intermediate products as may be aτ a low level during the process, in order to obtain a h process yield and to enable high, concentrations of starti substrate and catalyτic substance to be used. The object of the present inventionlis to provide novel and useful method of carrying out biochemical conve processes of the aforedescribed kind in which the afore¬ mentioned problems can be resolved in a satisfactory mann The characterizing features of the method according to the invention are set forth in- the accompanying claims . The invention will now be described in more detail with reference to the accompanying drawing, in which Figures 1A, IB och 1C illustrate schematically a non-continuous conversion process carried out in accordance with the method of the invention; Fig. 2 illustrates schematically a conversion process carried out continuously in accordance with the method of the invention; Figures 3A and 3B illustrate schematically a conversio process using a coenzyme-dependent enzyme when applying the method according to the invention; and Figures 4, 5 and 6 are diagrams illustrating "the resul obtained in tests carried out when applying the method according to the invention for converting cellulose to ethanol. In accordance with the invention, the conversion proces is carried out in a liquid system including at least two liqui phases which are so selected that the catalytic substance use is enriched in one of said phases. Figure 1A illustrates schematically one such biphase liquid system, including a bottom phase- Fl and a -top phase F2. The biphase system is so selected " that 'the catalytic substance K used, for example an enzyme present in solution, is enriched " in the bottom phase Fl. The starting substrate S for the conversion pjrocess may be uniformly distribu t ed in the biphases Fl and F2 , although ther may be selected to special advantage a system in which also the substrate S is enriched in the same phase Fl as the catalyti substance K, as illustrated in Figure 1A. In this case, the to phase F2 contains neither the catalytic substance K nor the starting substrate S. When carrying out the conversion process according to the invention, the liquid system is agitated so that the top phase F2 becomes finely dispersed in the bottom phase Fl , as illustrated schematically in Figure IB. The con- version process continues in the resultant dispersion or emulsion, within the fine droplets of the bottom phase Fl , as illustrated schematically in Figure 1C which shows one such droplet. The starting substrate S and the catalytic substance ' / K are both isolated in said droplets and are in extremely o π intimate contact with each other , thereby enabling the proce to continue without diffus ion limitations or steric hindran even though the starring substrate should be macromolecular particulate . In accordance with the invention , the two phase Fl and F2 in the liquid system are so selected that the -prod P formed in the conversion proces s is also distributed to th top phase F2 , as illus trated schematically in Figures IB and 1C. Thus , the product P pas ses to the top phase F2 as s aid product is formed in the convers ion proces s taking place in the finely divided droplets of the bottom phase Fl . In this way , it is pssible to maintain a low concentration of the product P in the bottom phase Fl , s.o as to enable any inhibit effect of the product on -the conversion: process to be elimin or at leas t greatly restricted . As illustrated schematically in Figure 1 , this is particularly the - case when the biphase liquid system is such that the top phase F2 has a subs tantial much greater volume than the bottom phase Fl , for example a volume which is ten times greater than the volume of s aid bottom phase . The distribution of the product P in the two phases Fl and F2 , i . e . the concentration of the products P in the .two phases , is not , in the maj ority of cases , appreciably affected by the ratio between the respective volumes of the two phases . Thus , il: is pos sible in this way for a much large amount of product P to be formed without the concentration of said* product in phase Fl increas ing to a value which would cause inhibition of the proces s . Consequently , it is possible to use the starting substrate and the catalytic substance in high concentrations and to achieve a high process yield at th same time . This favourable and sought after result can be sti further improved if , in accordance with a particularly advan¬ tageous embodiment of the invention , the phases of the liquid system are s elected so that the product P formed is enriched in the top phase F2 , so that the concentration of product P is much greater in the top phase F2 than in the bottom phase in which the convers ion process continues . In this way , the concentration of product P in the bottom phase Fl can be kept at such a low level as to have substantially no inhibiting effect on the process . The trans ition of product P from the bottom phase Fl to the top phase F2 becomes very effective when maintaining the bottom phase Fl in a finely dispersed— _ state in the top phase F2,.such as to provide a very large transition interface between the two phases. When the conversion process has terminated, agitation of the system is stopped, so as to .enable the two phases Fl and F2 to separate. In this liquid system, the catalytic substance K still remains concentrated in the bottom phase Fl together with any remaining, non-converted residues of the starting substrate S, while, if the biphase system is such that the product is partitioned to the top phase F2 , a large part of or substantially all the formed product P is present in the top phase F2. The top phase F2 can now be removed from the process chamber and treated in a suitable, conventional manner for recovering the product P from said phase. Liquid systems which comprise two or more phases and which can be used when carrying out the method according to the invention are, in themselves, well known and exhaustively described in the Literature. Thus, a biphase system which can be used when practicing the invention comprises mixtures of water and two mutually different polymers , which in this respect may both be non-ionic polymers or polyelectrolytes or a non-ionic polymer and a polyelectrolyte . Examples of such biphase systems are dextran-polyethylene gly col-water , dextran-Ficσll-water , dextran-methylcellulose-water , dextran- polyvinylalcohol-water, -Na dextran ' sulfate-polypropylene glycol-water , Na carboxymethyldextran-polyethylehe glycol-water with an addition of NaCl , Na-dextran sulfate- Na carboxymethyldextran-wate . A useable biphase system may also comprise a mixture of water, a polymer and a component of low molecular weight, for example a salt. Examples of such systems are polypropylene glycol-potassium phosphate-water, polyethylene glycol-potassium phosphate -water , polyethylene gly col-ammonium sulf te -water . A further account and comprehensive exemplification of aqueous biphase systems and polyphase systems which can be used in accordance with the invention is given in "Partition of Cell Particles and Macromolecules " , Per-Ake Albertsson, Wiley-Interscience , for example Tables 2.1 and 2.2 on pages 22, 23 and 25, and table 10.3 on page 261. Thus, there is a very large number of systems to chose from when selcting a liquid system which is suitable both with respect to the availability and price of the phase components- and with respect to the requirements placed on th conversion process in question. Because of the high water content of these aqueous biphase or polyphase systems , the systems may dissolve proteins and are in general more amenia to biological materials, such as enzymes, microorganisms and cells used as catalytic substances. The surface tension of t interface between the different phases is also very small, which means that the phases can be maintained finely mixed wiτh each other by agitation, without he energy consumed by said agitation becoming too significant. As mentioned in the "aforegoing, .the method according the invention affords particularly important advantages over previously known methods when using macromolecular or parti- culate starting substrates. It will be understood, however, that the method according to the invention can also be appli in conjunction with the use of different starting substrates for example gases or low molecular, soluble or insoluble substances . The product formed in the process may also be of different kind, such as a gas, a low-molecular, macromolecul or particulate substance, a-nd may be soluble or insoluble. Naturally, mixtures of different starting substrates may -als be used, and mixtures of different process products may be produced. The method according to the invention may be applied to particular advantage for carrying out continuous conversi processes. In this respect, as schematically illustrated in Figure 2, there can be used a reactor vessel 1 having a lowe part la, which serves as a mixing chamber and in which the t phases of the liquid system are maintained intimately mixed agitation, and an upper part lb, in which no stirring or agitation takes place and in which the two phases thus separ from each other so that only the top phase E2 is present in least the uppermost region of the part lb. This top phase F2 is removed, either intermittently or continuously, from the upper part of the reactor vessel through an outlet 2 , and is passed to a treatment unit 3, in which the formed process product can be removed from the top phase F2 in a suitable manner, said top phase then being returned to the lower part of the reactor vessel through an inlet 4. The process starti substrate may be supplied to the lower part la of the reacto vessel in a suitable manner, not shown in detail. 5 As before mentioned, -the.method according to the .invention can afford particularly important advantages over previously known methods when carrying out biochemical con¬ version processes with the use of an enzyme whose activity is dependent upon the presence of a coenzyme. As illustrated in 10 Figures 3A and 3B, in such a process, which may be carried o intermittently or, to advantage, continuously, there is adde to the biphase liquid system, in accordance with the inventi not only the starting substrate S-, for the conversion proces and the enzyme E, requisite therefor, but also the requisite 15 coenzyme CE and a further enzyme E_ , able to co-act to produce the necessary regeneration of the modified coenzyme CE , so that said enzyme returns to its original configuration CE . I this case the phase system is selected so that both the enzy E, and the coenzyme CE and the further enzyme E_ are partiti 20 to the bottom phase Fl. The product formed in the desired conversion process is- designated P-, in Figure 3B, while the ■ reference S„ identifies a substrate requisite for regenerati the coenzyme, and the reference P„ identifies a product forme when regenerating the coenzyme under the influence of the 25 enzyme E„ .. It may be necessary also to add the substrate S- to the liquid system, and the phases of the liquid system may if so is suitable, be selected so that the substrate S„ is partitioned to the bottom phase F. and that both products P_ and ?2 are partitioned to the top phase F„ . 30 The invention will hereinafter be further illustrated as applied to a process for the enzymatic degradation and con version of particulate cellulose to ethanol. This process proceeds in accordance with the following reaction chain : endoglucanases exoglucanases β-glucosidase yeast or -_ - , Oli• g & osaccharides . . ^ G„,lucose .. . . -E r+ t.- 35 cellulose > -?> ,> cellobiose In this chaih of reactions, the end product, ethanol , has a highly inhibiting effect on the preceding reaction stage while the intermediate product glucose, and still more the intermediate product cellobiose, have an inhibiting effect on respective preceding reaction stages . Tests have been carried out in accordance with the invention both on the first part of. the reaction chain, namely the enzymatic degradation of cellulose to glucose , and on the latter part of the reaction chain, namely the fermentation of glucose to ethanol, and also on the complete reaction chain from cellulose to ethanol. " Preparation of a biphasic system x The tests were carried out in an aqueous biphase system prepared by mixing a volume of Dextran T-40 (16 % weight/weigh (from Pharmacia Fine Chemicals , Uppsala) dissolved in a 0.1 M sodium acetate buffer, pH 4.5, with 5 volumes of PEG-6000 (12 % weight/weight) (polyethylene glycol having a molecular weight of 6000 from Union Carbide, N.Y. , USA) dis¬ solved in the same buffer. Test 1: Fermentation of glucose to ethanol The fermentation of glucose to ethanol when using-bake '-s y was investigated, by adding "100 mg of yeast to 60 ml of the biphase system prepared in accordance with the above, and sus¬ pending said yeast in the system, after which 0.5 grams of glucose was added. The system was agitated by means of a -magnetic agitator at a temperature of 30 C and samples (0.5 ml) were taken from the mixed phase system at regular intervals. These samples were centrifuged and the top phase analysed. The result can be seen from the diagram in Figure 4, where curve A shows the concentration of ethanol (mg/ml) in the top phase as a function of the reaction time (hours). Test 2 : Enzymatic degradation of cellulose to glucose The enzymatic degradation of cellulose to glucose was investigated by adding to 60 ml of the biphase system prepared in accordance with the above 0.5 gram of cellulose (Soϋ a Floe 200 from Brown Company, N.H. USA), 100 mg of cellulase (SP 122) (endoglucanases . and exoglucanases from Tricoderma Reesei; Nova AS, Copenhagen, Denmark) and 10 mg of β-glucosidase OMPI (E. C.3.2.1.21) (prepared from sweet almonds by P-L Biochemi Wis. , USA). The system was treated in the same manner as th recited in test 1. The result can be seen from the diagram Figure 5, where the curve B shows reducing sugars (mg/ml) a the curve C glucose (mg/ml) in the top phase as a function time (days ) . Test 3: Bioconversion of cellulose to ethanol The whole reaction sequence from cellulose to ethano was investigated by adding 100 mg yeast, 100 mg cellulase, 10 mg g-glucosidase and 0,5 g cellulose to 60 ml of the biphase system prepared in accordance with the above. Same conditions were used as: in tests 1 and 2. The result can be seen from the diagram in Fig. 6, where ' curve D shows ethano (mg/ml), curve E shows reducing sugars (mg/ml) and curve F shows glucose (mg/ml) in the top phase as a function of time (days) . Discussion of the test results As mentioned in the foregoing, it is. important when, carrying out the method according to the invention that the catalytically active substances are enriched in one of the phases. Consequently, there was first investigated the partition coefficients of the enzymes and the yeast cells, i.e. the concentration in the top phase relative to the concentration in the bottom phase. It was found that the yeast cells were strongly partitioned to the bottom phase (partition coefficient K < 0.5) and that the g-glucosidase was also partitioned to the bottom phase (partition coeffi¬ cient K = 0.25). On the other hand, the cellulases were more uniformly distributed between the two phases when they were present in a pure system, i.e. in the absence of cellulose in the system. When cellulose is added, however, the cellu¬ lases bind to the cellulose fibres and the agregate is found in the bottom phase. Thus, when cellulose is present in the system both the enzymes and the yeast cells are con- centrated in the same phase as the cellulose substrate, i.e. in the bottom -phase. The ability of the yeast cells to convert glucose to ethanol in the biphase system used in accordance with the invention was investigated in Test 1. As will be seen from curve A in Figure 4, there was obtained in the top phase after 24 hours approximately 2.5 mg/ml of ethanol. If a uniform distribution of glucose between the two phases is assumed, the initial concentration of glucose in the system was 8.3 mg/ If the ethanol is also uniformly distributed between the two phases, a complete conversion of glucose to ethanol would thus result in 4.25 mg/ml ethanol. Thus, after 24 hours the amount of ethanol obtained in the test was about 57 % of the theoretical yield. The enzymatic saccharification of cellulose to glucose in the biphase system used in accordance with the invention was investigated in Test 2. Because of the high inhibiting effect of primarily cellobiose, the enzymatic saccharification of cellulose is generally characterized by a high initial rate, whereafter the process of saccharification continues ver slowly. The curve- C in Figure 5 shows, however, that in the test carried out according to the inyention the glucose content increased constantly during the four days over which the experiment was continued. At the same time the amount of reducing sugars decreased still more strongly, which shows that substantially equal quantities of glucose and cellubiose were produced in the system. Both the amount of glucose and the amount of reducing sugars produced per unif of time decreased slowly, which indicates that inhibiting concentra¬ tions of both glucose and reducing sugars had accumulated during the latter part of the test. As a result of the favourable distribution in the biphase system used, i.e. catalysts and substrates concentrated in one phase while the products are more uniformly distributed,and the ratio between • the volumes of the two phases, i.e. the top phase has a volume which is five times greater than the bottom phase, large quantities of the products, however, passed to the top phase. Continuous removal of the products from the top phase would apparently provide a still more favourable system. The high concentrations of reducing sugars compared with the glucose C PI concentration confirms that the β-glucosidase activity was also subjected to product inhibition. The complete conversion process from cellulose to ethanol using the biphase system according to the inventio was investigated in Test 3. The result of this test, illustrated in the diagram in Figure 6, clearly shows that the amount of yeast added was excessive compared with the amount of glucose present, i.e. the enzymatic saccharifica of cellulose to cellubiose and glucose constituted the rate limiting reaction stage. Thus, less than 0.1 mg/ml glucose was present in the system during the whole of the test. Thi fact is also apparent when making a comparison between the curve D in Figure 6 and the curve A in Figure.4, which show that when converting pure glucose to ethanol there is obtai approximately twice as much ethanol as when converting gluc simultaneously produced from cellulose. A comparison betwee curve C in Figure- 5 and curve E in Figure 6 shows that the presence of yeast effectively reduces the glucose concentra tion of the system, thereby eliminating the product inhibit effect of the β-glucosidase activity. This is also clear fr the fact that the cellubiose concentration (estimated as • reducing sugars, curve E in Figure 6) passes a maximum and then decreases to a constant level of about 0.5 mg/ml. The fact that at this maximum level of concentration the reduci sugars are approximately ten times greater than the glucose concentration indicates a poor β-glucosidase activity. The test was carried out over five days , during whic no inhibiting effect of the ethanol could be observed. The ethanol production was substantially linear during the whole of the test period. The final value of about 5 mg/ml indica that a practically complete conversion of cellulose to ethan had been- reached at the end of the test, assuming an equal distribution of ethanol in the system. Thus , the tests carried out show that a bioconversion of cellulose to ethanol in a biphase system in accordance wi the present invention is very effective. Such a biphase syst also enables the ethanol formed to be removed continuously from the top phase of the system, whereby a continuous trans of ethanol from the bottom phase to the top phase is obtained, so that inhibiting concentrations of ethanol are prevented from occurring in the bottom phase, where the bio- conversion process takes place. Conventional batchwise 5 processes for manufacturing ethanol from cellulose by simultaneous saccharification and fermentation of the resultant glucose to ethanol have shown that the enzyme activity for cellulose degradation is inhibited to 50 % already at an ethanol concentration of 3 %. 10 In the following the method according to —he invention will be exemplified with a number of additional examples . Example 1. Production of acetone and butanol with the use of bacteria Clostridium acetobutylicum A biphase system was used consisting of 6 % (w/w) 15 Dextran T-40 as bottom phase and 25 % (w/w) PEG 8000 (polyethylene glycol with molecular weight 8000) as top phase. The volume ratio of the top phase to the bottom phase was 6:1. To this phase system were added 40 g/1 glucose as substrate and 10 g/1 peptone together with 10 g/1 yeast -extract as 20 nutrition medium for the bacteria Clostridium acetobutylicum, ATCC 824. The temperaturewas 35°C. The bacteria were enriched . in the bottom phase, whereas the substrate and the nutrition medium were distributed in both phases. 25 After about 30 hours the bacteria had produced 8 g/1 butanol and 3 g/1 acetone to be compared with 6 g/1 and 2 g/1, respectively, if no biphase system is used. Butanol and acetone can be distilled from the top phase and if more sugar is added to the system the production will ■ 30 start again. This will happen also if the top phase is replaced with a new top phase without any additional nutritive medium and more sugar is added to the system. Example 2. Production of polymer with the use of bacteria 35 If the fermentation in Example 1 is left to continue on its own, the butanol is after 4 days converted to a polymer, which is not yet fully defined but which seems to be poly- hydroxy butyric acid. The reason for this conversion is believed to be that the bacteria are present in an environ¬ ment having a reduced water activity whereby their meta- bolism is changed. Example 3. Production of penicillin A biphase system was used consisting of 6 % (w/w) Dextran T-40 , 7.5 % (w/w) PEG 8000 and 0,3 H TRIS buffer up to 100 % (w/w) , pH 7.8. A continuously operating reactor of the general type illustrated in Fig. 2 was used. The reactor volume was 6 ml of which volume 3 ml was agitated whereas the remaining 3 m was maintained unagitated. The volume ratio of the top phas to the bottom phase was 3:1. The bottom phase included 0,5 of a solution of penicillin acylase which was partitioned to the bottom phase. The top phase was pumped into the reactor at a rate of 1,8 ml/h.and included 70 g/1 benzyl penicillin which in the reactor was distributed in both phases. The reactor ' temperature was 37°C. The output- flow from the reactor contained 14 g/1- of 6-APA. " - Example 4. Hydrolysis with the use of inorganic catalyst A biphase system was used consisting of 6 % (w/w) : ' Dextran T-40 and 7.5 % (w/w) PEG' 8000. The volume ratio top phase to bottom phase was 3:1. To this system were added 0,1 g/ml of the superacid NAFION® (Dupont, USA) as catalyst, which was partitioned to the bottom phase, 5 % (w/w) cellobiose as substrate and 0,1 % (w/w) NaCl. Within 4 days the cellobiose was converte to 1,2 % (w/w) glucose. Example 5. Biphase system in which one phase is also substrate A phase system was used consisting of 15 % (w/w) PEG 800 and 15 % (w/w) starch, which had been geled at 73 C in the presence of 0 ,6 % (v/w) of the a ylase enzyme Termamyl^ (N0V0 A/S, Copenhagen). The bottom phase contained also 10% '(w/w) of yeast. The initial volume ratio top phase to bottom phase was 3:1. The starch was converted to 20 g/1 ethanol. Example 6. Use of enzyme and coenzyme A biphase system was used consisting of 6 % (w/w) PEG 20000 (molecular weight 20000), 10 % (w/w) Dextran T-10 (molecular weight 10000) and 0,03 M, pH 8,8, pyrophosphate buffer. The volume ratio top phase to bottom phase was 3:1, The enzyme was alcohol dehydrogenase, 50 units/ml in the bottom phase, and the coenzyme was NAD , 8 mM. As substrate was used 200 M ethanol, which was converted to 100 mM acetaldehyde. For the regeneration of the coenzyme one could use either a second substrate in the form of glutaraldehyde, at least 200 mM, or a second enzyme in the form of alamine dehydrogenase, 50 units/ml, or a flavine in the form of flavinemononucleotide, 12 mM. Example 7. Production " of the enzyme amylase with the use of Bacillus subtilis The same phase system as in Example 4- was used. As substrate one used 20 g/1 lactose and as nutritive medium 10 g/1 peptone and 10 g/1 yeast extract together with mineral salts. In this system Bacillus subtilis produced the enzyme. amylase after about 48 hours. The enzym was partitioned (enriched) to the top phase. Example 8. Production of acetic acid with the use of E. coli The same phase system as in Example 4 was used. As substrate one used 30 g/1 glucose. The nutritive medium was chosen according to Jano et al, J. Ferment. Technol. , Vol. 58, No. 3 (1380), p. 259. In this system E. coli produced about 5 g/1 acetic aci from 30 g/1 glucose. The acetic acid was partitioned (enriched) to the top phase. CϊfPl Example 9. Production of ethanol with the use of Thermoanaerobacter ethanolicus The same phase system as in Example 4 was used. As subsfrate one used not "more than 10 g/i of a sugar chosen according to Wiegel J. , Ljungdahl L.G., Arch. Microbiol., 128 (1981), p. 343. The nutritive medium was selected according to Wiegel J., Ljungdahl L.G., Rawson J.R., (1979) J. Bacterial. 139:800-810. As in this system the bacteria are partitioned (enric to the bottom phase, whereas the sugar is distributed in both phases, a larger total amount of sugar can be used in the system and converted to ethanol, without any inhibitin effect on the activity of Th. ethanolicus, due to a high sugar concentration. Example 10. Biological conversion in a polymer-salt system A biphase system was used consisting of 13,5 % (w/w) PEG 4000, 13,5 % (w/w) KgS0 4 7H 2 0 and 100 mM TRIS, HC1 buff pH 7,0. The volume ratio top phase to bottom phase was 3:1. As substrate one used 14 mM phenol, 0,8 mM amϊno antipyrine and 1 mM H 2 0 2 .As enzyme one used peroxidase, which was partitioned (enriched) to the bottom phase. In this system phenol and a ino antipyrine are convert to a conjugated aromatic called quinemine, which is par- titioned to the top phase. By means of this process it is possible to make phenol in e.g. waste water harmless. Example 11. Production of ethanol with the use of Zymomonas sp. The same phase system as in Example 4 was used. As substrate one used 10-25 % (w/w) glucose and the nutritive medium was selected according to Rogers P.L., Lee K.J., Tribe D.E. (1979) Biotechnol. Letters 4, p. 165-170 and p. 421-426. The use of the biphase system prevents the inhibiting effects of the substrate glucose as well as the product ethanol . It will be seen from the aforegoing that the method according to the present invention can be applied to great advantage when carrying out a number of different biologica and chemical conversion processes. Although one of the advantages with the method according to the invention is th it is possible to use non-immobilised catalytic substances, such as enzymes and microorganisms for example, and despite this retain these catalytic substances in the process chamber, it will be seen that there is nothing to prevent the invention from being applied while using immobilised catalytic substances , if so desired. The biphase or polypha liquid system used has been exemplified in the aforegoing with reference to a system consisting of aqueous phases, although it will be understood that systems based on liquid other than water can also be used if said liquids are compa tible with the starting substrate, catalytic substance and products present in the process in question. OMPI

Claims: Claims 1. A method of carrying out a biological or chemical conversion process which requires the presence of at least one catalytically active substance, characterized in that the process is effected in a liquid system in which a starting substrate (S) for the process and the catalytic substance (K) are introduced and which comprises at least " two liquid phases (Fl, F2) so selected that the catalytic substance (K) is enriched in one of said phases (Fl), at least part of the starting substrate (S) is present in said one phase (Fl) , and the process product (P) is partitioned also to the other of said phases (F2); and in that said liquid system is agitated so that said one phase (ED is held finely dispersed in said other phase (F2). 2. A method according to claim 1, characterized in that .the two phases are selected so that the process product (P is enriched in said other phase (F2). 3. A method according to claim 1 or claim 2, characteriz in that the two phases are selected so that the starting substrate (S) is enriched , in said one phase (Fl). 4. A method according to any one of -claims 1 - 3, characterizedin that said other phase (F2) has a substan¬ tially greater volume than said one phase (Fl). 5. A method according to any one of claims 1 - 4, characterized in that after the process is completed, agita tion is discontinued and the two phases allowed to separate whereafter said other phase (F2) is treated for the recover of the process product (P) therefrom. 6. A method according to claim 4, characterized in that agitation of the liquid system is effected in a manner such that said one phase (Fl) is held dispersed in only a portio (la) of said other phase (F2), and in that part of the remaining portion (lb) of said other phase (F2), is removed from the process chamber, either intermittently or continuously, and treated for the removal of process product from said part, and then returned to the process chamber. 7. A method according to any one of claims 1 - 6, for carrying out a conversion process in which the catalytic substance is an enzyme (E-, ) which requires the presence of a coenzyme (CE) , characterized in that the coenzyme (CE) and a further enzyme (E 2 ) capable of regenerating said coenzyme are also added to the liquid system, the two phases of which are selected so that the coenzyme (CE) and said further enzyme (E2) are also enriched in said one phase (Fl) . 8. A method according to any one of claims 1 - 7, characterized in that the two phases of .said liquid system comprise, two mutually different aqueous polymer solutions. 9. A ' method according to any one of claims 1 — 7, characterized in that the irwo phases of said liquid system comprise an aqueous polymer solution and an aqueous salt solution. OMPI

Inventors: ALBERTSSON PER AKE, HAEGERDAL BAERBEL, MATTIASSON BO
Applicants: ALBERTSSON PER AKE, HAEGERDAL BAERBEL, MATTIASSON BO
Application year: 1981
Main classification: C12P1
IPC codes: B01J14, C02F1, C02F3, C12J1, C12M1, C12N9, C12P1, C12P7, C12P19, C12P37, C13K1