N-ARYL-N'((1,4,5,6-TETRAHYDROPYRIMIDIN-2-YL) OR (4,5,6,7-TETRAHYDRO-1-H-1, 3-DIAZEPIN-2-YL)UREAS FOR INTESTINAL DISORDERS AND AS ANTIHYPERTENSIVES

Abstract: N-(substituted phenyl)-N′-(1,4,5,6-tetrahydropyrimidin-­ 2-yl) or (4,5,6,7-tetrahydro-1-H-1,3-diazepin-2-yl) ureas and pharmaceutically-acceptable salts thereof are disclosed to be useful in relieving the symptoms associated with irritable bo­ wel syndrome, and as anti-diarrheal agents and as antihyper­ tensives.

Description: N-Aryl-N'-[(1,4,5,6-Tetrahydropyrimidin-2-yl) or (4,5,6,7 tetrahydro-l-H-1,3-diazepin-2-yl)]Ureas for Intestinal Disorders and as Antihypertensives Field of Invention This invention relates to a method for controlling hypertension, a method for relieving the symptoms associated with irritable bowel syndrome and a method for controlling diarrhea employing certain N-aryl-N"-[(1,4, 5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H- 1,3-diazepin-2-yl)]ureas, to certain of said urea compounds per se, and to pharmaceutical compositions containing said urea compounds. Background of the Invention Irritable bowel syndrome is a functional disorder characterized primarily by abdominal pain, but also by diarrhea and constipation and like symptoms. It is believed that the pain associated with this syndrome is due to excessive sensitivity to distention of the bowel caused either by intestinal gas and/or fecal material. Treatments which would provide relief from the discomforts associated with such symptoms and/or the disorder which produce such symptoms are highly desirable. Brief Summary of the Invention The present invention involves a method of reducing arterial pressure in hypertensive animals,a method for relieving the symptoms associated with irritable bowel syndrome and a method for controlling diarrhea in animals having said syndrome or in animals having diarrhea, respectively, by administering to said animal in a pharmaceutically-acceptable carrier, a therapeutically effective amount of a compound, or a pharmaceuticallyacceptable salt thereof, having the formula: EMI2.1 In the foregoing and subsequent formulas, Ar is a phenyl radical of the formula: EMI2.2 wherein X is Br or C1, and Y is Br, C1 or CH3; and n is 0 or 1. This invention also involves, as compounds per se, the compound wherein X and Y are each C1 which is the most active compound for the above methods involving intestinal disorders, and its pharmaceutically-acceptable salts, and the compound and its salts wherein X is C1, and Y is CH3. This invention also embraces compositions, suitable for use in the above antihypertensive method, wherein the foregoing urea compound is in admixture with a pharmaceutically acceptable carrier. The most preferred composition contains the compound wherein X and Y are each C1, which is the most active antihypertensive compound, or its pharmaceutically-acceptable salts. The activities of the above compounds reside in the urea base so that useful acid addition salts may be from various acids provided only that the acids be pharmaceutically-acceptable. Representative acid salts include hydrochloride, hydrobromide, phosphate, sulfate, P toluenesulfonate, benzenesulfonate, malonate, succinate, methosulfate, methanesulfonate, 2-napsylate and the like. Prior Art Certain N-(aryl)-tetrahydropyrimidin-2-yl ureas are generically disclosed in U.S. Patent No. 3,168,520, but the only utility taught is in connection with dyeing, and no pharmaceutical utility is taught, nor is any of the compounds used in the present invention specifically disclosed. An article by G. H. Douglas, et al., Arzneimittel Forschung., Vol. 28 (II) Supplement 8a, 1435-1441 (1978) discloses as Compound 109 at p. 1438 (in its tautomeric form) the compound of the formula: EMI3.1 which was disclosed to be active as a gastric acid antisecretory agent. The article does not teach the antihypertensive activity, the activity as an antidiarrheal agent or in relieving the symptoms associated with irritable bowel syndrome for this 2,6-dimethylphenyl compound, which applicant found to exist. Prior Art The above article by G. H. Douglas, et al., Arzneimittel Forschung., Vol. 28 (II) Supplement 8a, 1435-1441 (1978) erroneously discloses as Compound 109 at p. 1438 (in its tautomeric form) the compound of the formula: EMI4.1 which was disclosed to be active as a gastric acid antisecretory agent. The article does not teach the antihypertensive activity, the activity as an antidiarrheal agent or in relieving the symptoms associated with irritable bowel syndrome for this 2,6-dimethylphenyl compound, which applicant found to exist. The columns headed by R2 and R3 in Table 3 on p. 1438 of said article were transposed erroneously leading to the above structure, which was then disclosed in Chem Abstracts, 90, 48236s (1979), where it was named in the Chem. Abstracts, Vol. 90, 1979 - Formula Index and given Chem. Abstracts Registry Number. Method of preparation The pharmacologically useful N-aryl-N'-(1,4,5,6-tetrahydropyrimidine-2-yl)urea compounds are prepared from 2-amino-1,4,5,6-tetrahydropyrimidine HCl, which has the structure: EMI4.2 and which is a known compound (R. F. Evans and D. J. Brown, J. Chem. Soc., 4039 (1962) prepared according to the following reaction scheme: EMI5.1 EMI5.2 <tb> <SEP> H <tb> <SEP> 0 <tb> (Ir)free <SEP> basetArNCO <SEP> solvent <SEP> -- <SEP> (III) <tb> <SEP> &verbar; <SEP> HX <tb> <SEP> Acid <SEP> addition <SEP> salts <SEP> of <SEP> (I) <tb> The pharmacologically useful W-aryl-N'-(4,5,6,7-tetrahydro- lH-1,3-diazepin-2-yl)urea compounds are prepared from 2-amino-4,5,6,7-tetrahydro-lH-1,3-diazepine II, which has the structure: EMI5.3 and which is a known compound. EMI6.1 <tb> <SEP> H <SEP> H <tb> C' <SEP> H3C"' <SEP> HNO <tb> <SEP> N <SEP> N <SEP> 2. <SEP> ENO <SEP> n <SEP> trate <tb> <SEP> Inwn <tb> <SEP> solsmnt <tb> <SEP> (It(b) <tb> <SEP> H <tb> (II) <SEP> + <SEP> ArNCO <SEP> solvent <SEP> a <tb> <SEP> L.bNE' <SEP> il <tb> <SEP> E <tb> <SEP> HX <tb> <SEP> 8 <SEP> t <tb> <SEP> Acid <SEP> addition <SEP> salts <SEP> of <SEP> (I) <tb> The free base form of II can be generated by treating a stirring suspension of II HC1 in solvents such as CH2C12 (methylene chloride), tetrahydrofuran, dioxane, and the like with strong bases such as aqueous NaOH (50%), concentrated KOH, and the like; NaOH (50%) being preferred. The resulting solution of free base II is dried over a suitable drying agent such as Na2SO4 or K2C03. The dried solutions may either be used as such for reactions with aryl isocyanates to obtain products I or the extraction solvent may be evaporated in vacuo and the residual free base II taken up in a different organic solvent, e.g., DMF, DMSO, and the like; said solutions of II then being treated with an appropriate isocyanate III to afford the free base products I. Although the reactions of II with III may be carried out with equimolar amounts of reac tants, usually a stoichiometric excess, generally of from about 0.25-1.0 mole of free base II to that of aryl isocyanate III is employed in order to minimize undesired side reactions such as, for example, formation of bis-aryl isocyanate adducts with II. Temperature ranges for those reactions may conveniently range from about -200 to 700C. The products I, obtained in free base form, may conveniently be purified by dissolving in an organic solvent, immiscible with H20, such as CH2C12, washing with H20 to remove excess II, if any, followed by isolation of I from the solvent by drying, filtration from drying agent, and solvent removal. Alternatively, the reactions of II free base with III may be carried out by adding a solution of II HC1 in DMF, DMSO, and the like, to a stirring suspension of a stoichiometric amount of an alkali metal hydride such as LiH, NaH, and the like, LiH being preferred, which forms II free base, the corresponding alkali metal chloride, and H2 gas. The thus obtained solution of free base II (the presence of the metal chloride does not interfere with the subsequent reaction) is treated with an appropriate amount of aryl isocyanate III. When the reaction is complete, dilution with H20 or ice-H20, in excess amounts, causes precipitation of crude product I and leaves any unreacted II in solution. Filtration then allows isolation of crude I. Said I, in free base form, may be further purified, if necessary, by recrystallization and chromatographic techniques, and so forth, according to standard techniques known in the art. A further purification method may be used such as dissolution in dilute aqueous acid, such as HC1, most preferred, H2S04, HBr, HN03 and the like, filtration from any undissolved impurities, followed by neutralization with suitable inorganic bases such as sodium and potassium bicarbonates and carbonates and the like, dilute alkali metal hydroxides such as NaOH, KOH, and the like, and organic bases such as triethylamine, diisopropylethylamine, and the like, which causes precipitation of I free base. The thus-obtained free base I may then be purified by recrystallization, etc., as described above, or may be converted to a suitable pharmaceuticallyacceptable salt form- of I which also may be purified by recrystallization or precipitation techniques well known in the art. Said pharmaceutically-acceptable salt forms of I are generally comprised of I in combination with suitable mineral acids such as HC1 (most preferred), HBr, H2SOk, H3P04, and strong organic acids such as benzenesulfonic, p-toluenesulfonic, 1- and 2-naphthalenesulfonic, ethanedisulfonic, methane- and ethanesulfonic methylsulfuric, and the like, being the most preferred. Although salts of I with weaker acids, such as benzoic, fumaric, maleic, citric, etc. do form, they are relatively easily dissociated because of the relatively weak base strength of I. This dissociation may be caused by attempted drying in vacuo, dissolution in H20, etc. The ease of dissociation, however, may not necessarily preclude use of salts of this type in pharmaceutical formulations insofar as they remain stable enough to be purified by recrystallization, etc., and capable of being formulated into pharmaceutical preparations such as tablets, capsules, and the like. The preferred salt forms of I are additionally capable of forming hydrates and solvates with H20 and certain organic solvents, respectively. Also, I and its salt forms may exist in several tautomeric forms. It is naturally intended that the various hydrates, solvates, and tauto meric forms of I be included within the scope of this invention. The N-aryl-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H-l,3-diazepin-2-yl)i urea compounds have been found to be useful as anti-diarrheal agents and to provide relief from the discomfort associated with functional intestinal disorders spoken of as irritable bowel syndrome. The disorder is characterized by abdominal pain, diarrhea, constipation and like symptoms caused by excessive sensitivity to distension of the bowel by the presence of intestinal gas and/or fecal material. The N-(substituted phenyl)-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-1-H-1,3-diazepin-2-yl)] urea compounds useful in the present invention have been found to alleviate hypertension and further, to generally accomplish this without an accompanying increase in heart rate. The compounds most useful in the present invention do not show an increase in heart rate, but a lowering of heart rate and, generally, long duration. An agent which has an antihypertensive effect without increasing but rather maintaining or decreasing heart rate, is the one considered most useful for beneficially treating a hypertensive subject. The extent to which a compound possesses these properties may be primarily determined in the antihypertensive test hereinafter described. Rodent Antihypertensive Screen--This test evaluates compounds for effects on arterial pressure and heart rate. In this test, the arterial pressure of adult spontaneously hypertensive rats [SHR] (Charles River) is monitored directly via an aortic cannula. Rats are anesthetized with an inhalation anesthetic (methoxyflurane). The left carotid artery is isolated and cannulated. The tip of the cannula is advanced to the aorta and the cannula is exteriorized behind the neck at the level of the scapula. Animals are placed in individual cases and allowed to recover from the anesthetic and are kept unrestrained. The arterial cannula is connected to the pressure transducer which is attached to the recorder. Heart rate is determined from the arterial pressure recording. The test compounds are administered either orally (p.o.) by gavage or by intraperitoneal (i.p.} injection. The arterial pressure and heart rate are monitored for a minimum of 24 hours. A test compound is considered to be active as an antihypertensive agent if the mean arterial pressure (MAP) indicates a fall of > 15 mm of Hg. Each animal serves as his own control. The results of this test employing at least 3 rats per dose level for each compound and performed with N-aryl-N' (1,4,5,6-tetrahydropyrimidin-2-yl))urea compounds are shown in Table I. The results seen in Tables 1A and 1B show that N-(substituted phenyl)-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H-l,3-diazepin-2-yl)] urea compounds and their salts possess not only the beneficial antihypertensive property but also the desirable property of maintaining or lowering heart rate. The compounds used in the present invention are useful for treating hypertension (high blood pressure) by administer ing to subjects in need of treatment, a therapeuticallyeffective hypertension reducing amount of a N-(substituted phenyl)-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6, 7-tetrahydro-l-H-l, 3-diazepin-2-yl)] urea compound of Form ula I or its pharmaceutically-acceptable salt as active agent. The active agents may be administered with or without carrier in the amounts hereinafter set forth. A pre ferred method of administration is by the use of pharmaceu tical compositions in unit dosage form as described below. The operable ranges for carrying out the treatment is the administration, orally or parenterally, of from about 1 milligram to about 500 milligrams of said N-(substituted phenyl)-N'-[(l,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H-l,3-diazepin-2-yl)J urea compound in dosage unit form. While the therapeutic method is most useful for human subjects, it may also be employed for other mammals. Operable amounts are generally within the range of from about 0.5 to 100 mg/kg of body weight. TABLE 1-A ANTIHYPERTENSIVE AND CARDIAC RATE DETERMINATIONS EMI12.1 SHR Rat MAP in #mm Hg #HR in Duration Ar McN- x (mg/kg,route) beats/min. in hours 2,6-Cl2Ph 4951(base) - -54 (35 p.o.) -86 21 2,6-Cl2Ph 4951(base) - -79 (100 p.o.) -114 > 24 2,6-Cl2Ph 4951(base) - -58 (30 p.o.) -140 > 24 2,6-Cl2Ph 4951(base) - -78 (30 i.p.) -100 > 24 2,6-Cl2Ph 4951(base) - -41 (10 p.o.) -76 > 24 2,6-Cl2Ph 4951(base) - -42 (3 p.o.) -36 17 2,6-Cl2Ph 4951(base) - -24 (1 p.o.) -44 1 2-CF3Ph 5027-11-98 1 -49 (30 i.p.) -110 11 -65 (100 p.o.) -74 11 2,6-Me2Ph 5028-11-98 1.1 -70 (30 i.p.) -126 23 art compound -82 (100 p.o.) -168 > 24 2-Cl-6-MePh 5058-11-98 0.5 -73 (30 i.p.) -103 > 24 -66 (100 p.o.) -118 > 24 2,6-Br2-4-FPh 5042-11 0 -90 (30 i.p.) -60 17 -37 (100 p.o.) -71 11 MAP = Mean Arterial Pressure # = Change HR = Heart Rate TABLE 1-B ANTIHYPERTENSIVE AND CARDIAC RATE DETERMINATIONS EMI13.1 SHR Rat #MAP in mm Hg #HR in Duration in Ar X McN- (mg/kg, route) beats/min. Hours 2,6-Cl2Ph 0.5 4967-11-98 -49(35 p.o.) -78 21 -50(30 p.o.) -124 > 24 -48(10 p.o.) -67 23 -31(1 p.o.) -64 17 2-CF3Ph 0 5036-11 -43(30 i.p.) +39 > 1 -39(100 p.o.) -98 11 2,6-Me2Ph 1/3 5076-11-98 -94(30 i.p.) -127 > 24 -64(100 p.o.) -112 > 24 2-Cl-6-MePh 1/3 5075-11-98 -81(30 i.p.) -108 17 -58(100 p.o.) -104 23 2,6-Br2-4-F Ph 0 5037-11 -30(30 i.p.) +58 1 -43(100 p.o.) +41 23 2,6-(OMe)2Ph 0 5040-11 -87(30 i.p.) +62 1 -79(100 p.o.) -50 11 MAP = Mean arterial pressure # = Change HR = Heart Rate Definition of "irritable bowel syndrome--this condition also known as irritable colon, spastic colon, mucous colitis, is a clinical disorder characterized by either diarrhea or constipation, in association with abdominal discomfort, but without demonstrable structural bowel disease. It is classified as a functional bowel disease. In practice, it is one of the major digestive disorders and is a leading cause of absenteeism from work and a reason for physician office visits. In modern usage, nirritable bowel syndrome" is the preferred term for this condition. Pathophysiology--Although the etiology is unknown, recent studies have elucidated characteristics of the pathophysiology of this condition. Alteration of colonic motility has been suggested as a mechanism of this syndrome. This is characterized by an increased basal activity, as well as a sensitivity to exogenous or endogenous stimuli. Since the symptoms many times are brought on by eating, it has been postulated that cholecystokinin, which is released from the small intestine after eating is partially responsible. Electrical recordings from the human colon have also shown that there is a difference in the characteristic slow wave frequency in patients with irritable bowel. Patients with irritable bowel syndrome have a lower pain threshold to distention than normal. Thus, they are much more sensitive to the distention caused by normal amounts of gas or fecal contents present in the colon. If the sensitivity to distention can be reduced, the pain associated with irritable bowel syndrome necessarily will be reduced. Irritable bowel appears to be unique to man and no naturally-occurring disease similar to irritable bowel has been discovered in animals. In order to test compounds for possible activity in this state, the pathophysiologic characteristics of the disease must be utilized in designing animal experiments to test such compounds. The "glass bead test" described below has been developed to test for the effectiveness of compounds against one component of this disease state, that of response of the colon to distention, which could produce abdominal pain. Although no way of producing an increased sensitivity to distention of the bowel has been found in the mouse, stimulation by a greater than normal distending force is utilized. A drug interfering with this response to distention may be of use in the treatment of irritable bowel syndrome if it has other characteristics which could provide efficacy. The "glass bead test" can also be used to evaluate antidiarrheal activity. Although most drugs producing constipation in man are effective in the glass bead test, many work by mechanisms which do not fulfill the characteristics of an agent which both decreases the response of an agent which both decreases the response to distention and effects abnormal motor activity. One example is that of narcotic analgesics which are effective in the glass bead test, but increase the phasic motor activity of the intestine and are not useful in irritable bowel. Other test models should, therefore, be used to confirm or further define the utility of any compound as an anti-irritable bowel agent. One test to confirm the utility of compounds effective in the glass bead test, which may be used as an indicator of effectiveness for the diarrhea state produced by irritable bowel syndrome is the "castor oil diarrhea test" in mice or rats. (A description of this test is given infra.) The series of compounds for which there is claimed antiirritable bowel activity in fact are active against castor oil diarrhea, the effective doses correlating well with those which are effective in the glass bead test. Since there is no model for the constipation phase of this condition, the regularizing action of a compound cannot be examined. However, since constipation in irritable bowel syndrome is often secondary to diarrhea, one would expect that the prevention of diarrhea should alleviate the constipation phase. The Glass Bead Test: The extent to which a compound is effective in providing relief from the symptoms associated with irritable bowel syndrome may be determined by a test in which a glass bead is inserted into the rectum and the lapse time between insertion and expulsion of the bead determined. Compounds which are effective in decreasing the sensitivity to distention of the colonic wall delays the expulsion of the bead. The test is carried out with male albino mice of 18-25 grams body weight using groups of five mice for each compound dose tested. The initial screen dose selected for all compounds is 50 milligrams per kilogram of body weight (mg/kg) administered orally in a volume of 0.1 milliliter per 10 grams of body weight. The control groups receive the vehicle, 0.5 per cent methocel, used for both oral and intraperitoneal administration. The mice are fasted one hour before testing and the test drugs are given one hour prior to glass bead insertion. At the end of the pretreatment time, the mouse is picked up and held firmly in one hand with his abdomen facing the technician. The glass bead of 3 millimeters in diameter is positioned at the rectum and using a pinching action with a thumb and forefinger, the bead is pushed into the rectum. Then using a glass rod of 3 millimeters in diameter which has been lubricated with 0.5 per cent methocel to facilitate insertion, the glass bead is pushed up into the rectum a distance of 2 centimeters using a slow gentle turning motion. The mice are timed as a group using the last mouse inserted as zero time and the number of beads expelled in a group at different timed intervals are recorded. The groups are based on timed intervals of o to 5 minutes, 5 to 10 minutes, 10 to 20 minutes, 20 to 40 minutes, and greater than 40 minutes. They are given the activity index values of 0, 1, 2, 3, and 4, respectively. Mice who have not expelled their beads by the 40minute cutoff time are examined for perforations. Those mice whose colons are perforated are eliminated from the group. The sum of the values divided by the number of mice or beads is termed the activity index for the drug tested. The ED50 is determined by regression lines using the method of last squares. The ED50 is arhitrarily assigned as that dose causing an activity index of 2. The ED2 is essentially equivalent to the ED50, with the activity being expressed on a scale of 0 to 4 in the Glass Bead Test. The results of this test employing intraperitoneal and oral administration of N-aryl-N'-[(1,4,5,6-tetrahydro- pyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H-1,3-diazepin-2- yl)]urea compounds are shown in Tables 2A and 2B. Tables 2A and 2B also contain results of the Mouse Castor Oil Method Test described below. TABLE 2-A EMI18.1 M O U S E M=5-10 M=5 Glass Castor oil Bead Test Diarrhea Test ED2P.O. mg/kg p.o. Ar x McN- mg/kg 1 hour 5 hours 2,6-Cl2Ph - 4951(base) 9.4 1.6 3.9(ED50) 2,6-Cl2Ph 0 4951-11 7 mg/kg p.o. 6.5(ED50) 4.3 mg/kg 2-CF3Ph 1 5027-11-98 > 200 100 > 100 200 lethal (4/5) 2,6-Me2Ph 1.1 5028-11-98 28.6 < 50 21.5(ED50) art compound 2-Cl-6-Me2Ph 0.5 5058-11-98 < 6.25 - 18 2,6-Br2-4-FPh 0 5042-11 > 200 50 145 TABLE 2-B EMI19.1 Mouse n=5-10 3n=5 Glass Bead Test Castor Oil Diarrhea ED2P.O. mg/kg P.O. Ar x McN- mg/kg 1 hour 5 hours 2,6-Cl2Ph 0.5 4967-11-98 9.7 6.25 10.4(ED50) 2-CF3Ph 0 5036-11 146 50 > 100 200 lethal (4/5) 2,6-Me2Ph 1/3 5076-11-98 22.3 - 41(ED50) art compound (disclosed, but not actually made) 2-Cl-6-MePh 1/3 5075-11-98 16.2 - 26(ED50) 2,6-Br2-4-FPh 0 5037-11 91.4 50 108(ED50) 2,6-(OMe)2Ph 0 5040-11 200 50 > 100 200 lethal# (4/5) Mouse Castor Oil Method Test--Male albino mice weighing 18-30 gms are employed for this study. The mice are fasted overnight before testing; however, water is given ad lib. during fast. On the day of testing, the mice are weighed beforehand to obtain an average weight using an individual weight range of +5 gms. Drugs are administered by the desired route (p.o., s.c., or i.p.) suspended or dissolved in 0.5 per cent methocel using a volume of 1 ml/100 gm of average body weight (0.1 ml/10 gm) to groups of five mice for each compound dose tested. Control mice receive the test vehicle. One hour after, a single dose of castor oil (0.3 ml per mouse) is given orally to all groups. The mice are returned to their cages with access to food and water for the remainder of the experiment. Mice fasted overnight will have diarrhea usually within one hour after administration of castor oil. Observations for the presence of diarrhea are taken eve-y hour for five hours. A positive or negative response is used. The absence of diarrhea is expressed in percent inhibition as compared to the incidence of diarrhea at that time period in the control group. ED501s are determined by calculation regression analysis using a least square method. The effect over the total duration of the experiment is used for this calculation. The foregoing results in Tables 2-A and 2-B illustrate the beneficial effect of N-aryl-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-l-H-1,3-diazepin-2- yl)lurea compounds in decreasing abnormal sensitivity to distention of bowel. These properties are utilized in the methods and compositions of the present invention. The process of the present invention, namely, a method for alleviating functional intestinal disorders and for treating the discomforts associated therewith comprises administering to subjects in need of treatment, a therapeutically-effective amount of an N-aryl-N'-(1,4,5,6tetrahydropyrimidin-2-yl)urea compound of Formula I or its pharmaceutically-acceptable salt as active agent. The active agents may be administered with or without carrier in the amounts hereinafter set forth. A preferred method of administration is by use of pharmaceutical compositions in unit dosage form as described below. The operable ranges for carrying out the process is the administration, orally or parenterally, of from about 1 milligram to about 500 milligrams of an N-aryl-N' [(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7 tetrahydro-1-H-1, 3-diazepin-2-yl)] urea compound in dosage unit form. While the therapeutic method is most useful for human subjects, it may also be employed for other mammals. Operable amounts are generally within the range of from about 0.50 to 100 mg/kg. The outstanding properties are most effectively utilized by use of the pharmaceutical compositions of the present invention. The pharmaceutical compositions comprising an N-aryl-N'-(l,4,5,6,-tetrahydropyrimidin-2-yl)urea compound or acid addition salt thereof, as the active ingredient, may be prepared by intimately mixing the urea compound with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques, which carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral. In preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed. Thus, for liquid oral preparations, such as for example, suspensions, elixirs and solutions, suitable carriers and additives include water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like; for solid oral preparations such as, for example, powders, capsules and tablets, suitable carriers and additives include starches, sugars, diluents, granulating agents, lubricants, binders, disintegrating agents and the like. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are employed. If desired, tablets may be sugarcoated or enteric-coated by standard techniques. For parenterals, the carrier will usually comprise sterile water, though other ingredients, for example, for purposes such as aiding solubility or for preservation, may be included. Injectable suspensions may also be prepared, in which case appropriate liquid carriers, suspending agents and the like may be employed. The term "dosage unit form" as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets, capsules, pills, powder packets, wafers, teaspoonsful, tablespoonsful, and the like, and segregated multiples thereof. A dosage unit generally will contain from about 10 to about 500 mg of the N-aryl-N'-(1,4,5,6-tetrahydropyrimidin-2-yl)urea compounds, for use in intestinal disorders, and from about 1 to 500 mg of said compounds for use as antihypertensives. The following examples illustrate the preparation of the urea compounds suitable in the practice of the invention, but are not to be construed as limiting. EXAMPLE I-A N-(2,6-Dichlorophenyl)-N'-(1,4,5,6-tetrahydropyrimi- din-2-yl) urea A solution of 4.20 g (0.0424 mol) of 2-aminotetrahydropyrimidine in 30 ml of dry DMF was warmed to 700C. and with stirring there was added 7.16 g (0.0381 mol) of 2,6dichlorophenyl isocyanate in 25 ml of dry DMF over a period of 0.5 hours. After stirring for 2 hours at 700C., the reaction mixture was cooled, diluted with H2O and filtered. The filter cake was washed well with H2O and air dried. The crude product, 5.1 g (47%) was dissolved in CH2C12, dried over K2CO3, filtered through a prewashed pad of filter aid and hexane was added to the cloud point. The resulting crystals were filtered, washed with hexane and dried in vacuo for 3 hours (500C.; 5 mm Hg) to give pure N-(2,6-dichlorophenyl)-N'-(1,4,5,6-tetrahydropyrimi- din-2-yl)urea, 4.71 g (43%); m.p. 179-180.50C. TLC: 5x20 cm silica gel; toluene-ether-MeOH (8:4:1) or CHCl3-MeOH- NH40H (90:10:1) indicated homogeneity; IR (KBr) 3421,1628 cm¯l; W max. (MeOH) 222 nm (±= 28300) and 239 nm (inflection) (±= 18,000). ANAL. Calcd. for CllHl2Cl2N4O: C, 46.01; H, 4.21; N, 19.51. FOUND: C, 45.98; H, 4.23; N, 19.48. The starting 2-aminotetrahydropyrimidine was prepared according to the procedure of R. F. Evans, D. J. Brown, J. Chem. Soc., 4039 (1962), which was liberated from the hydrochloride with excess 50% NaOH, extracted with CH2C12, dried (K2CO3) and evaporated in vacuo to an oil. EXAMPLE I-B N-(2,6-Dichlorophenyl)N'-(1,4,5,6-tetrahydlropyrimidin2-yl)urea Monohydrochloride A mixture of 8.50 g (0.0625 mol) of 2-amino-1,4,5,6tetrahydropyrimidine hydrochloride, 10.0 g (0.12 mol) of 50% NaOH and 70 ml of THF was stirred for 0.5 hours at room temperature and then 10.0 g Na2SO4 was added. After stirring for an additional 0.5 hours, a solution of 9.4 g (0.050 mol) of 2,6-dichlorophenyl isocyanate in 50 ml of THF was added over a period of 1 hour. After stirring for 1 hour, the reaction mixture was filtered, the filtrate evaporated in vacuo and the residue dissolved in 25 ml of 10% HC1 and 25 ml of H2O with warming. After filtration through a pad of diatomaceous earth, chilling in ice gave the crystalline hydrochloride which was recrystallized from MeOH-ether affording 12.40 g. This material was ground to a fine powder and dried to constant weight in vacuo (60 C., 5 mm Hg) to give 11.97 g (74%) of the title compound; m.p. 215-217 C. dec., homogeneous by TLC [5x20 cm silica gel GF CHCl3-MeOH-NH4OH (90:9:1)]. 1H-NMR (DMSO-d6) 1.85 [pentet, (poorly resolved)2H]; 3.35 (t, J=5HB, 4H); 7.2-7.7 (m, 3H); 9.16 (broad s, 2H) exchangeable; 10.02 (broad s, 1H) exchangeable, 11.19 (brS, 1H) exchangeable. IR(KBr) 3125, 1717, 1678, i628 cm¯l; W max. (MeOH) 241 infl. (e= 11,200) and 220 nm infl. (±= 23,500). ANAL. Calcd. for CllHl2Cl2N4O HC1: C, 40.83; H, 4.05; N, 17.31. FOUND: C, 40.78; H, 4.05; N, 17.30. EXAMPLE 2 N-(2-Chloro-6-Methylphenyl)-N'-(1,4,5,6-tetrahydropyrimidin-2-yl) urea Monohydrochioride Hemihydrate A mixture of 8.14 g (0.060 mol) of 2-amino-1,4,5,6- tetrahydropyrimidine hydrochloride, 6.0 g (0.075 mol) of 508 NaOH and 75 ml of THF was stirred for 0.5 hours at room temperature and then 15 g Na2 S04 was added. After stirring for 0.5 hours, a solution of 6.70 g (0.040 mol) of 2-chloro-6-methylphenyl isocyanate in 50 ml of THF was added over a period of 0.5 hours. After stirring for 1 hour, the reaction mixture was filtered, the filtrate evaporated in vacuo and the residue dissolved in 25 ml of 10% HC1 and 25 ml of H2O with warming. After filtration through diatomaceous earth chilling in ice gave the crystalline hydrochloride which was recrystallized from cold H2O after treatment with charcoal affording 7.15 g (59%). This material was dried in vacuo(200C., 5 mm Hg) to constant weight to give 7.11 g (59%) of pure N-(2chloro-6-methylphenyl)-N'-(1,4,5,6-tetrahydropyrimidin2-yl)urea monohydrochloride hemihydrate, m.p. (190) 2152200C. melts, then forms a new solid; m.p. 243-2450C.; IR(CHC13) 3224, 1713, 1677, 1641, 1543 cm-Ú; W max. (MeOH) 236 ( 16,200) and 217 nm infl ( 21,500). ANAL. Calcd. for C12H15ClN4OHClO5H2O: C, 46.17; H, 5.49; N, 17.94; H2O, 2.89. FOUND: C, 46.21; H, 5.53; N, 17.75; H2O, 3.14. EXAMPLE 3 According to the teachings of Examples 1-2, the following compounds are prepared: 1. N-(2,6-Dibromophenyl)-N'-(1,4,5,6-tetrahydro- pyrimidin-2-yl)urea and acid addition salts. 2. N-(2-Bromo-6-chlorophenyl)-N'-(1,4,5,6-tetrahydro- pyrimidin-2-yl)urea and acid addition salts. 3. N-(2-Bromo-6-methylphenyl)-N'-(1,4,5,6-tetrahydro- pyrimidin-2-yl)urea and acid addition salts. EXAMPLE 4 1,000 hard gelatin capsules, each containing 200 milligrams of N-(2,6-dichlorophenyl)-N'-(1,4,5,6-tetra- hydropyrimidin-2-yl)urea are prepared from the following formulation: Grams N- (2, 6-Dichlorophenyl ) - '-(1,4,5,6-tetrahydropyrimidin-2-yl)urea 200 Starch 250 Lactose 750 Talc 250 Calcium Stearate 10 A uniform mixture of the ingredients is prepared by blending and employed to fill two-piece hard gelatin capsules. The capsules are suitable to be orally administered to subjects with functional bowel disorders, and to hypertensive subjects to reduce blood pressure. EXAMPLE 5 Gelatin capsules are prepared as described in Example 4 except that in the formulation, (for use in intestinal disorders) 400 grams of N-(2,6-dibromophenyl)-N'-(1,4,5,6- tetrahydropyrimidin-2-yl)urea is employed as active agent providing capsules containing 400 milligrams of N-(2,6 dibromophenyl)-N'-(1,4,5,6-tetrahydropyrimidin-2-yl)urea. EXAMPLE 6 Gelatin capsules are prepared as described in Example 5 except that N-(2-chloro-6-methyl)-N'-(1,4,5,6-tetrahydro- pyrimidin-2-yl)urea is employed as the active agent. EXAMPLE 7 1,000 compressed tablets, each containing 500 milligrams of N-( 2-chloro-6-methylphenyl)-N'-( 1,4,5, 6-tetrahydro- pyrimidin-2-yl)urea are prepared from the following formulation: Grams N-(2-Chloro-6-methylphenyl) '-(1,4,5,6-tetrahydropyrimidin-2-yl)urea 500 Starch 750 Dibasic calcium phosphate hydrous 5,000 Calcium stearate 2.5 The finely powdered ingredients are mixed well and granulated with 10 per cent starch paste. The granulation is dried and compressed into tablets using starch as a disintegrant and calcium stearate as a lubricant. EXAMPLE 8 Gelatin capsules are prepared as described in Example 7 except that N-(2,6-dibromophenyl)-N'-(1,4,5,6-tetrahydro- pyrimidin-2-yl)urea is employed as the active agent. EXAMPLE 9-A 2-Amino-4,5,6,7-Tetrahydro-lH-1,3-DiÅazepine Mononitrate A suspension of 217.9 g 2-(methylthio)-4,5,6,7-tetrahydro lH-l,3-diazepine hydroiodide in 400 ml of MeOH was treated with anhydrous NH3 (NaOH traps + NaOCl traps) vigorously for 1 hour. All the solid dissolved. The solution was saturated with NH3 and let stand over the weekend at room temperature. TLC showed the reaction to be essentially complete. The MeOH was boiled off replacing with t-BuOH then cooled and Et2O added gave the HI salt (hygroscopic). The material was converted to the free base in CH2C12. The organic layer was dried (K2CO3), filtered and the solvent removed i vacuo affording a heavy oil. The oil was taken up in absolute EtOH and carefully neutralized with HNO3 (conc. aqueous). A little solid impurity precipitated, early on, which should have been removed and discarded. Before the acid had all been added, the mixture became too thick to mix thoroughly. A first crop of the salt was collected and washed with fresh EtOH (95%). The filtrate was then neutralized to pH7 affording a second crop. Recrystallization of these crops was a long and tedious process using MeOH-EtOH (95%) then boiling off the MeOH and filtering hot from less soluble inorganic impurities. After many recrystallizations, a crop of quite pure product was obtained, 24.7 g (12.8%); m.p. 165 157 C., less pure crop; m.p. 156-161.5 C., 20.9 g (10.8%); IR (KBr) 3251, 3108, 1660, 1598 cm'l; NMR. ANAL. Calcd. for C5HllN3 HNO3 (113.16/176.175): C, 34.09; H, 6.87; N, 31.80. FOUND: C, 34.28; H, 6.36; N, 31.59. EXAMPLE 9-B N-(2,6-Dichlorophenyl)-N'-(4,5,6,7-tetrahydro-1H1,3-diazepin-2-yl)urea Monchydrochloride Hemihydrate A 300 ml flask was charged with 12.1 g (0.05 mol) of amino-4,5,6,7-tetrahydro-lH-l,3-diazepine hydroiodide 1), 10.0 g of 50% NaOH and 70 ml of THF. After stirring at room temperature for 1 hour, 10.0 g Na2SO4 was added and the mixture was stirred for an additional 1.5 hours. The reaction mixture was cooled to -300C. (2-PrOH, dry ice) and a solution of 6.3 g (0.033 mol) of 2,6-dichlorophenyl isocyanate in 80 ml of THF was added over a period of 2 hours. At the end of addition, the reaction mixture was allowed to warm to room temperature and stirring continued for 1 hour. The solids, were filtered and the filtrate evaporated in vacuo. The residue was dissolved in dilute HC1, insolubles removed by filtration, and the aqueous solution washed with ether, made basic with cold 30% NaOH, and extracted with CH2Cl2. The combined organic layers were dried (K2CO3), filtered through a pad of diatomaceous earth and evaporated in vacuo. The residue was dissolved in MeOH, neutralized with ethereal HC1 and treated with ether to give crystals which were recrystallized from MeOH/ether and then from a minimum amount of H2O to give pure N-92,6-dichlorophenyl0-N'-(4,5,6,7-tetrahydro-1H-1,3diazepin-2-yl)urea monohydrochloride hemihydrate; m.p. (175) 177-1790C. dec.; W max. (MeOH) 244 (ç20,100) and 224 nm shld (e20,800); IR (RBr) 3420, 3200, 1710, 1675, 1535 cm-Ú. ANAL. Calcd. for C11H14C12N4OHC1O5H2O: C, 41.59; H, 4.65; N, 16.16; H2O, 2.60. FOUND: C, 41.88; H, 4.70; N, 16.12; H2O, 2.92. P. Stefanye and William L. Howard, J. Am. Chem. Soc., 77, 761(1955) and Brit. Pat. 612,693, Nov. 16,1948; Chem. Abstr., 43, 6670d (1949). Material dried in vacuo at 50 (5 mm Hg) 4 hours (to constant weight). EXAMPLE 10 According to the teaching of Example 9-B, the following compounds are prepared: 1. N-(2-Chloro-6-methylphenyl)-N'-(4,5,6,7-tetrahydro- lH-1,3-diazepin-2-yl)urea monohydrochloride; m.p. 1591610C. dec. 245-2500C. 2. N-(2,6-Dibromophenyl)-N'-(4,5,6,7-tetrahydro-lH-1,3- diazepin-2-yl)urea monohydrochloride and acid addition salts (solvates). 3. N-(2-Bromo-6-chlorophenyl)-N'-(4,5,6,7-tetrahydro-lH1,3-diazepin-2-yl)urea monohydrochloride and acid addition salts (solvates). 4. N-(2-Bromo-6-methylphenyl)-N'-(4,5,6,7-tetrahydro-lH 1,3-diazepin-2-yl)urea monohydrochloride and acid addition salts (solvates). The following examples illustrate the preparation of various pharmaceutical compositions useful for the present invention: EXAMPLE 11 1,000 hard gelatin capsules, each containing 200 milligrams of N-(2,6-dichlorophenyl)-N'-(4,5,6,7 tetrahydro-lH-l, 3-diazepin-2-yl) urea are prepared from the following formulation: Grams N-(2,6-Dichlorophenyl)-N'-(4,5,6,7 tetrahydro-lH-1,3-diazepin-2-yl)urea 200 Starch 250 Lactose 750 Talc 250 Calcium stearate 10 A uniform mixture of the ingredients is prepared by blending and employed to fill two-piece hard gelatin capsules. The capsules are suitable to be orally administered to subjects with functional bowel disorders. EXAMPLE 12 Gelatin capsules are prepared as described in Example 11 except that in the formulation, 400 grams of N-(2,6 dibromophenyl)-N'-(4,5,6,7-tetrahydro-lH-1,3-diazepin-2- yl)urea is employed as active agent providing capsules containing 400 milligrams of N-(2,6-dibromophenyl)-N' (4,5,6,7-tetrahydro-lH-1,3-diazepin-2-yl)urea. EXAMPLE 13 Gelatin capsules are prepared as described in Example VII except that N-( 2,6-dibromophenyl)-N'-(4,5,6,7-tetrahydro- lH-1,3-diazepin-2-yl)urea is employed as the active agent. EXAMPLE 14 1,000 compressed tablets, each containing 500 milligrams of N-(2-chloro-6-methylphenyl)-N'-(4,5,6,7-tetrahydro-lH- 1,3-diazepin-2-yl)urea are prepared from the following formulation: Grams N-(2-Chloro-6-methylphenyl)-N'-(4,5,6,7-tetra- hydro-lH-1,3-diazepin-2-yl)urea 500 Starch 750 Dibasic calcium phosphate hydrous 5,000 Calcium stearate 2.5 The finely powdered ingredients are mixed well and granulated with 10 per cent starch paste. The granulation is dried and compressed into tablets using starch as a disintegrant and calcium stearate as a lubricant.

Claims: CLAIMS: 1. An N-(phenyl)-N'-[(1,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6,7-tetrahydro-1-H-l,3-Jiazepin-2-yl)]urea having the formula: EMI33.1 or pharmaceutically-acceptable salt thereof, wherein n is o or 1, X is Br or Cl and Y is Br, C1 or CH3. 2. N-(2,6-Dichlorophenyl)-N'-(1,4,5,6-tetrahydropyrimidirn 2-yl)urea and pharmaceutically-acceptable salts thereof. 3. N-(2-chloro-6-methylphenyl)-N'-(1,4,5,6-tetrahydropyrimidin-2-yl)urea and pharmaceutically-acceptable salts thereof. 4. N-(2,6-Dichlorophenyl)-N'-(1,4,5,6-tetrahydropyrimidiro-1H-1,3 diazepin-2-yl)urea, and its pharmaceutically-acceptable salts. 5. N-(2,6-Dichlorophenyl)-N'-(1,4,5,6-tetrahydropyrimidiro-1H-1,3diazepin-2-yl)urea, and its pharmaceutically-acceptable salts. 6. N-(2-chloro-6-methylphenyl)-N'-(4,5,6,7-tetrahydro-lH l,3-diazepin-2-yl)urea, and its pharmaceutically-acceptable salts. 7. A pharmaceutical composition in dosage unit form for treating hypertension, irritable bowel syndrome or diarrhea, selected from the group consisting of (a) an N (phenyl)-N'-[ (l,4,5,6-tetrahydropyrimidin-2-yl) or (4,5,6, 7-tetrahydro-1-H-1,3-diazepin-2-yl)]urea having the formula EMI34.1 wherein n is O or 1, X is C1 or Br, and Y is C1, Br or CH3; and (b) a pharmaceutically-acceptable salt thereof. 8. A pharmaceutical composition according to Claim 7 in which the active urea compound is N-(2,6-dichlorophenyl) N'-(1,4,5,6-tetrahydropyrimidin-2-yl)urea; or a pharamceutically-acceptable salt thereof. 9. A pharmaceutical composition according to Claim 7 in which the active urea compound is N-(2,6-dichlorophenyl) N'-(4,5,6,7-tetrahydro-1H-1,3-diazepin-2-yl0urea; or pharmaceutically-acceptable salt thereof. 10. A process for preparing an N-(phenyl)-N'-[(1,4,5,6,- tetrahydropyrimidin-2-yl) or 94,5,6,7-tetrahydro-1-H-1,3 diazepin-2-yl)Jurea having the formula EMI34.2 or pharmaceutically-acceptable salt thereof, wherein n is 0 or 1, X is Br or C1 and Y is Br, C1 or CK3; characterized by reacting a compound of the formula EMI35.1 wherein n is O or 1, with a compound of the formula: EMI35.2 in- a suitable solvent, at temperatures between -200C and 700C, and, if desired, preparing a pharmaceuticallyacceptable acid addition salt thereof. 11. A compound according to any one of Claims 1 to 6, or a composition according to any one of Claims 7 to 9, for use in treating hypertension, irritable bowel syndrome, or diarrhea.

Inventors: RASMUSSEN, CHRIS ROYCE
Applicants: MCNEILAB, INC.
Application year: 1981
Main classification: C07D239
IPC codes: C07D239, C07D243