1-N-ACYLATED AND 1-N-ALKYLATED DERIVATIVES OF 4-0-SUBSTITUTED -2-DEOXYSTREPTAMINE AMINOGLYCOSIDES

Abstract: Novel 1-N-acylated or 1-N-alkylated derivatives of a 4-O-substituted-2-deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, α or β-(C₁ to C₆ alkyl)aprosaminide, α or β-(C₂ to C₆ hydroxyalkyl)aprosaminide, α or β-(C₂ to C₆ aminoalkyl)aprosaminide, α or β-(C₂ to C₆ protected aminoalkyl) aprosaminide, α or β-(C₃ to C₆ aminohydroxyalkyl)-aprosaminide, α or β-(C₃ to C₆ (protected amino)hydroxyalkyl)aprosaminide, and pharmaceutically-acceptable acid addition salts thereof are disclosed. They are useful for treating warm blooded animals suffering from bacterial diseases. They and 1-N-acylated and 1-N-alkylated deriva­ tives of lividamine, paromamine and the 6′-N-protected derivatives of nebramine, neamine, gentamine C₁, genta­ mine C 1a , gentamine C₂, gentamine C 2a , and gentamine C 2b , and pharmaceutically-acceptable acid addition salts thereof can be prepared by reacting the corresponding aminoglyco­ side substrate with a zinc salt Zn (X₂), X being C₁ to C₁₀ carboxylate, in water, diluting with a polar organic solvent, treating with an alkalating agent or acylating agent, optional­ ly, deprotecting the amino or N-protected substituents, optionally, reducing carbonyl portions of an acyl group, and recovering as a free base or as a pharmaceutically-­ acceptable acid addition salt.

Description: 1-N-ACYLATED AND 1-N-ALKYLATED DERIVATIVES OF 4-O-SUBSTITUTED-2-DEOXYSTREPTSMINE AMINOGLYCOSIDES Novel l-N-acylated and lN-alkylated derivatives of 4-0-substituted-2-deoxystreptamine aminoglycosides have antibacterial activity and are useful in pharmaceutical, veterinary and surface disinfectant formulations. They and 1-Nacylated and l-N-alkylated derivatives of related aminoglycosides are prepared with the help of zinc salts. Examples of 4-O-substituted 2-deoxystreptaminecontaining aminoglycosides used in the process of the present invention include the antibiotics apramycin (see U.S. Patent Nos. 3,691,279, 3,853,709 and 3,876,767) EMI1.1 and nebramine: EMI2.1 The nomenclature of the above formula of apramycin is simplified by the following numbering system in which the carbons of the 2-deoxystreptamine moiety are numbered 1 through 6, the carbons of the octose moiety are numbered with a single prime, 1'-8', and the carbons of the hexose moiety are numbered with a double prime, 1"-6". EMI2.2 Analogously, the aprosaminide aminoglycosides referred to in this invention have the same numbering system as apramycin with respect to the octose and 2-deoxystreptamine moieties: EMI3.1 The numbering system for the pseudodisaccharides referred to in this invention, such as nebramine, has the carbons of the 2-deoxystreptamine moiety numbered 1-6, the same as for apramycin and the aprosaminides, but the hexose carbons are numbered with a single prime, 1'-6'. EMI3.2 The use of transition metal cations to selectively protect certain amino groups in aminoglycoside antibiotics from acylation has been reported by the following groups of workers: T.L. Nagabhushan, Abstr. 9th Int. Symp. Carbohyd. Chem., papers B36 and B37 (1978); T.L. Nagabhushan, et al., J. Am. Chem. Soc., 100, 5253 (1978); T.L. Nagabhushan, W.N. Turner and A. Cooper, U.S. Patents 4,136,254 (1979) and 4,230,847 (1980). S. Hanessian and G. Patil, Tet. Letters, 1031 and 1035 (1978). R.E. Carney and J.B. McAlpine, Abstr. 175th ACS Meeting, Carb. 46 (1978). R.E. Carney and S. Hanessian, U.S. Patent No. 4,214,077 (1980). T. Tsuchiya, Y. Takagi and S. Umezawa, Tet. Letters, 4951 (1979). According to the present invention there is provided a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, 3'-hydroxyapramycin, a or D-(C1 to C6 alkyl)aprosaminide, a or D-(C2 to C6 hydroxyalkyl)aprosaminide, a or D-(C2 to C6 aminoalkyl)aprosaminide, a or D-(C2 to C6 protected aminoalkyl)apros aminide, a or B-(C3 to C6 aminohydroxyalkyl) aprosaminide, a or D-(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmaceutically-acceptable acid addition salt thereof; ; wherein the acyl or alkyl substituent group is selected from the group consisting of y-amino-a-hydroxybutyryl, y-protected amino a-hydroxybutyryl, B-amino-a-hydroxypropionyl, ss-protected amino-a-hydroxypropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3 carbonyl, N-protected 3-hydroxypyrrolidine 3-carbonyl, 3-hydroxyazetidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl)acetyl, N-pro tected 2-hydroxy-2- (azetidin-3-yl) acetyl, 4-amino-2-hydroxybutyl, 3-amino-2-hydroxy - propyl, 2-aminoethyl, 3-hydroxypyrrolidine 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl)ethyl, C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl. This invention also provides formulations, pharmaceutical, veterinary and adapted as a surface disinfectant, which comprise as active ingredients l-N-acylated or l-N-alkylated derivatives of 4-0 substituted-2-deoxystreptamine aminoglycosides as above described excepting compounds bearing protected amino or N-protected substituents associated with at least one carrier or diluent thereof, as appropriate to the formulation. This invention also provides a process for preparing a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as before described or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine C1al gentamine C2, gentamine C2a, and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: : apramycin, 3' -hydroxyapramycin, a or B-(C1 to C6 alkyl)aprosaminide, a or ss-(C2 to C6 hydroxyalkyl)aprosaminide, a or ss-(C2 to C6 protected aminoalkyl) aprosaminide, a or ss-(C3 to C6 (pro tected amino) hydroxyalkyl) aprosaminide, lividamine, paromamine and the 6'-N- protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2b;. with between two and six molar equivalents of a zinc salt of the formula: Zn(X)2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then carrying out the reaction at a temperature between OOC. and 400C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used is a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, D-protected aminoa-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used are C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide ana substituted benzyl iodide. (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; and (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. The invention also provides l-N-acylated or a l-N-alkylated derivative of a 4-0-substituted-2 deoxystreptamine aminoglycoside as before described for use in treating warm-blooded animals having bacterial infection and for use as a surface disinfectant. The process of this invention involves regioselective acylation or alkylation of the l-amino group of 4-O-substituted-2-deoxystreptamine aminoglycoside substrates. The instant process is carried out by adding an acylating agent or alkylating agent to a mixture of the aminoglycoside substrate and a C1 to C10 carboxylate salt of zinc (II) in an aqueous polar organic solvent, and stirring the mixture for the appropriate time at a temperature between 0 C. and 400C. The aminoglycoside substrates that are used in the process of this invention are selected from a group consisting of apramycin, 3'-hydroxyapramycin, a or -(C1 to C6 alkyl)aprosaminide, a or D-(C2 to C6 hydroxyalkyl)aprosaminide, a or 5-(C2 to C6 protected aminoalkyl)aprosaminide, and a or ss-(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a and gentamine C2b. The acylating agents for use in the present invention are reactive derivatives of acyl moieties selected from the group consisting of y-protected amino-a-hydroxybutyryl, B-protected amino-a-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3carbonyl and N-protected 2-hydroxy-2-(azetidin-3-yl)acetyl. The alkylating agents used in the instant process are C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. Compounds claimed in the present invention include the above mentioned apramycin, 3'-hydroxyapramycin and the various a or 9-aprosaminide moieties, all substituted on the l-amino group by the above-listed acyl or alkyl moieties. As isolated from the process of this invention, these acylated compounds have their 1-N substituent in the protected amino or N-protected form. These derivatives in the protected amino and N-protected forms are intermediates to their respective deprotected amino and N-deprotected antibiotic compounds; which antibiotic compounds are also claimed in the instant application. Additionally, the deprotected amino acyl derivatives and the N-deprotected acyl derivatives may undergo reduction of the carbonyl group to give a methylene group. The deprotected amino acyl derivative compounds and the N-deprotected compounds and all of the 1-N alkyl derivatives, either in their free base form or their pharmaceutically acceptable acid addition salt form, are antibacterial agents and are also claimed compounds in the instant invention. This invention is directed to the 1-Nacylated and l-N-alkylated derivatives of apramycin, 3'-hydroxyapramycin, a or R-(C1 to C6 alkyl)aprosaminide, a or h-(C2 to C6 hydroxyalkyl)aprosaminide, a or D- (C2 to C6 aminoalkyl)aprosaminide, a or -(C2 to C6 protected aminoalkyl)aprosaminide, a or ss-(C3 to C6 aminohydroxyalkyl)aprosaminide, a or -(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, and the pharmaceutically acceptable acid addition salts of these compounds. The acyl substituent on the l-amino group of the above compounds is selected from a group consisting of y-amino-a-hydroxybutyryl, y-protected amino-a-hydroxybutyryl, B-amino-a-hydroxypropionyl, B-protected amino-a-hydroxypropionyl, glycyl, Nprotected glycyl, 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, 3hydroxyazetidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl)acetyl and N-protected 2-hydroxy-2-(azetidin-3-yl)acetyl. Alkyl substituents on the l-amino group of the above compounds are selected from the group consisting of the reduced carbonyl derivatives of the above deprotected amino acyl substituents and N-deprotected acyl substituents, namely: 4-amino-2-hydroxybutyl, 3-amino2-hydroxypropyl, 2-aminoethyl, 3-hydroxypyrrolidine3-methylene, 3-hydroxyazetidine-3-methylene, 2hydroxy-2-(azetidin-3-yl)ethyl, in addition to the alkyl substituents C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl. In the above description of the invention, the term "C1 to C6 alkyl means a branched or straight chain alkyl such as methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, sec-butyl, t-butyl, n-pentyl, amethylpentyl, n-hexyl and like groups. Similarly, by the term "C2 to C6 hydroxyalkyl", we mean a C2 to C6 alkyl chain substituted by from 1 to 3 hydroxyl groups, provided that no more than one oxygen atom is bonded to any single carbon atom of the alkyl chain. Examples of the hydroxyalkyl chains include 2-hydroxyethyl, 2-hydroxy-n-propyl, 3-hydroxy-n-propyl, 1,3-dihydroxy-2 propyl, 2, 4-dihydroxy-n-butyl, 3-hydroxy-2-methylpropyl, 2,4,5-trihydroxy-n-pentyl, 2, 4-dihydroxy-n-pentyl, 2-hydroxy-4-methylpentyl, 5-hydroxy-n-hexyl, 4,5,6trihydroxy-n-hexyl, and like groups. By the term "C2 to C6 aminoalkyl", we mean a C2 to C6 alkyl chain substituted by from one to three amino groups provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these aminoalkyl chains include: 2-aminoethyl, 2-amino-n-propyl, 3-amino-n-propyl, 1,3-diamino-2-propyl, 2, 4-diamino-n- butyl, 3-amino-2-methylpropyl, 2,4,5-triamino-n-pentyl, 3-amino-n-pentyl, 2-amino-4-methylpentyl, 5,6-diamino-nhexyl, 3-amino-n-hexyl, and like groups. By the term "C2 to C6 protected aminoalkyl", we mean a C2 to C6 alkyl chain substituted by from one to three protected amino groups, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these protected aminoalkyl chains include the example compounds listed above for the C2 to C6 aminoalkyl chains wherein each of the amino groups are substituted by N-protecting groups, and like compounds. By the term "C3 to C6 aminohydroxyalkyl", we mean a C3 to C6 alkyl chain substituted bv onca amino grout and one hydroxyl group, or by two amino groups and one hydroxyl group, or by one amino group and two hydroxyl groups, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these aminohydroxyalkyl chains include: 2-hydroxy-3-amino n-propyl, l-hydroxy-3-amino-2-propyl, 2-hydroxy-4amino-n-butyl, l-hydroxy-4-amino-2-butyl, 2-hydroxy-5amino-n-pentyl, 4 , 5-diamino-3-hydroxy-n-pentyl, 3,5dihydroxy-4-amino-n-pentyl, 2-amino-3-hydroxy-4methylpentyl, 2-hydroxy-6-amino-n-hexyl, and like groups. By the term "C3 to C6 (protected amino)hydroxyalkyl", we mean a C3 to C6 alkyl chain substituted by one protected amino group and one hydroxyl group, or by two protected amino groups and one hydroxyl group, or by one protected amino group and two hydroxyl groups, provided that no more than one heteroatom is bonded to any single carbon atom of the alkyl chain. Examples of these (protected amino)hydroxyalkyl chains include the examples listed above for the C3 to C6 aminohydroxyalkyl chains wherein each of the amino groups-are substituted by N-protecting groups, and like compounds. The terms "protected amino" and l'N-protected" used in the above description refer to an amino group substituted with one of the commonly employed amino blocking groups that are compatible with the present reaction conditions. Representative suitable aminoblocking groups for the purpose of this invention are described by J.W. Barton in "Protective Groups in Organic Chemistry," J.F.W. McOmie, Ed., Plenum Press, New York, N.Y., 1973, Chapter 2 and T. W. Greene "Protective Groups in Organic Synthesis," John Wiley and Sons, New York, 1981, Chapter 7. Preferred amino blocking groups are the various alkyloxycarbonyl and aryloxycarbonyl groups, with the most preferred amino blocking group being benzyloxycarbonyl. By the term "pharmaceutically acceptable acid addition salts," we mean those formed by standard acid-base reactions between the appropriate aminoglycoside and organic or inorganic acids. Such acids include hydrochloric, sulfuric, phosphoric, acetic, succinic, citric, lactic, maleic, furmaric, palmitic, cholic, pamoic, mucic, D-glutamic, d-camphoric, glutaric, glycolic, phthalic, tartaric, lauric, stearic, salicylic, methanesulfonic, benzenesulfonic, sorbic, picric, benzoic, cinnamic and like acids. By the term "C2 to C4 alkyl" we mean ethyl, n-propyl and n-butyl. By the term "C3 to C5 allyl" we mean allyl1 2-methylallyl, 3,3-dimethylallyl, 3-methylallyl and 2,3-dimethylallyl. Those skilled in the art will recognize that the latter two allyl substituents can exist in either the E or Z geometric isomers. In the present invention, it is understood that the allyl compounds can exist in the pure E or Z form, or as a mixture the two isomers. By the term "substituted benzyl" we mean a benzyl moiety where the phenyl ring is substituted with one substituent selected from the group consisting of fluoro, chloro, bromo, iodo, methyl, methoxy and trifluoromethyl. This substituent can be in either the ortho, meta or para positions. Examples of these substituted benzyl groups include para-chlorobenzyl, para-bromobenzyl, para-iodobenzyl, para-methylbenzyl, para-methoxybenzyl, para-trifluoromethylbenzyl, metafluorobenzyl, meta-bromobenzyl, meta-methylbenzyl, meta-methoxybenzyl, meta-chlorobenzyl, ortho-chlorobenzyl, orth-iodobenzyl, ortho-methylbenzyl, orthomethoxybenzyl, ortho-bromobenzyl, and the like. Those skilled in-the art will recognize that all of the above listed acyl substituents except glycyl and N-protected glycyl, the corresponding reduced acyl substituents, namely: 4-amino-2-hydroxybutyl, 3amino-2-hydroxypropyl, 3-hydroxypyrrolidine-3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin3-yl)ethyl, as well as many of the substituents described by the terms ''C1 to C6 alkyl", "C2 to C6 hydroxyalkyl", t'C2 to C6 aminoalkyl", "C2 to C6 protected aminoalkyl1,, "C3 to C6 aminohydroxyalkyl" and "C3 to C6 (protected amino)hydroxyalkyl" can exist as optical isomers. These terms refer to either the individual R or S isomers or to equal or unequal mixtures of the R and S isomers at each of the asymmetric carbon atoms for the purposes of this invention. The process of making the above l-N-acylated and l-N-alkylated compounds is the other aspect of this invention. Specifically, this process substantially selectively acylates or alkylates the l-amino group of the following substrates: apramycin, 3'-hydroxyapramycin, a or to C6 alkyl)aprosaminide, a or -(C2 to C6 hydroxyalkyl)aprosaminide, a or 5-(C2 to C6 protected aminoalkyl)aprosaminide, a or D-(C3 to C6 (protected amino)hydroxyalkyl) aprosaminide, lividamine, paromamine, and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2aI and gentamine C2b, by (1) combining the above aminoglycoside with between two and six molar equivalents of a zinc salt of the formula: : Zn(X)2 in water; (2) diluting the above mixture with between two and ten volumes of a polar organic solvent; and (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above dilution of step (2) then carrying out the reaction at a temperature between 0 C. and 400C. for a period between 0.5 and 24 hours for acylation, and between 15 hours and 60 hours for alkylation. The terms "C1 to , alkyl", "C2 to C6 hydroxyalkyl", "C2 to C6 protected aminoalkyl", "C3 to C6 (protected amino)hydroxyalkyl", "protected amino and "...N-protected..." are as defined above for the compounds of the instant invention. Four of the above-listed aminoglycoside substrates for the process of this invention, namely apramycin, 3'-hydroxyapramycinr lividamine and paromamine, are known compounds and therefore their synthesis or isolation can be found in the literature. The pseudodisaccharides nebramine, neamine, gentamine C1, gentamine Clal gentamine C2, gentamine C2aI and gentamine C 2b are also known compounds and therefore their synthesis or isolation can also be found in the literature. These pseudodisaccharides can be converted to their 6'-N-protected derivatives which are required as substrates for the instant process by methods analogous to the procedure given in Example 4, Part A of the Experimental Section. The various substituted a or ss aprosaminide substrates listed above can be made by methods analogous to the procedure described by S. O'Connor, et al., Journal of Organic Chemistry, 41, 2087 (1976). The acylating agents used in the instant process are also known in the art. Additional examples for converting the known carboxylic acid precursors into suitable acylating agents for the process of this invention are given in Preparations 1 and 2 in the Experimental Section. As with the compounds claimed in the present invention, it will be recognized by those skilled in the art that certain of the acylating agents, except for the reactive derivative of N-protected glycyl, certain C1 to C6 alkyl chains and certain substituted C2 to C6 alkyl chains and C3 to C6 alkyl chains of the a or ss substituted aprosaminide substrates can exist as optical isomers. For the purposes of the process of this invention, these moieties can be used as their pure R or S configurations or as mixtures of equal or unequal proportions of these two configurations at each of the asymmetric carbon atoms. In the above description of the process of the instant invention, the formula: Zn(X)2 refers to a zinc salt wherein the zinc is in the formal (II) oxidation state, and X refers to a C1 to C10 carboxylate ligand. The term "C1 to C10 carboxylate" includes straight or branched chain aliphatic carboxylates, as well as aromatic carboxylates, with acetate being the preferred carboxylate. Exemplary zinc salts used in the instant process include: Zn (acetate) 2 Zn (propionate) 2 Zn (hexanoate) 2 Zn(2-ethylhexanoate)2 Zn (benzoate) 2 Zn (octanoate) 2 Zn (neodecanoate) 2 Although not explicitly depicted in the above formula and examples, the zinc salts used in the process of this invention may be either anhydrous or hydrated. The term "acylating agent", as used in the above description of the instant process, means a reactive derivative of a carboxylic acid which is capable in the instant process of coupling the acid to an amino group by an amide linkage. The carboxylic acids that are converted into reactive derivatives for use as acylating agents in the process of this invention are selected from a group consisting of y-protected amino-a-hydroxybutyric acid, B-protected amino-a-hydroxypropionic acid, N-protected glycine, N-protected 3-hydroxypyrrolidine-3-carboxylic acid, N-protected 3-hydroxyazetidine-3-carboxylic acid, and N-protected 2-hydroxy-2-(azetidin-3-yl)acetic acid. Examples of reactive derivatives of a carboxyl group are an acid halide, an acid anhydride, an activated ester, an acid azide and the like. Preferred reactive derivatives are acid anhydrides and activated esters, with the more preferred reactive derivatives being acid anhydrides and the activated esters formed with Nhydroxysuccinimide and N-hydroxyphthalimide. After the l-(N-protected or amino-protected) acyl derivative compounds have been prepared by the instant process, they can be converted to their corresponding deprotected acyl derivatives by procedures well known in the art. Furthermore, the above deprotected amino or deprotected nitrogen acyl derivative compounds may have their carbonyl groups reduced to methylene groups with diborane or lithium aluminum hydride; these newly formed alkyl derivative compounds still retain biological activity. Methods for reducing the carbonyl groups are well known in the art, and include methods such as the ones described by K. Igarashi, et al. in U.S. Patent 4,201,774, herein incorporated by reference. An additional reference for the above reduction procedure is K. Richardson et al. J. Antibiotics, 30, 843 (1977). The alkylating agents used in the instant process are C2 to C4 alkyl bromide, C2 toC4 alkyl iodide, C3 to C5 allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. The preferred alkylating agents are ethyl iodide, n-butyl bromide, n-butyl iodide, allyl bromide, allyl iodide and benzyl bromide. It should be noted that the instant process will result in the l,6'-di-N-acylation of nebramine, neamine, gentamine C1, gentamine C1a, gentamine C2, gentamine C2a, and gentamine C2b when the 6'-amino group of these substrates is not protected. Therefore, in order to acylate the above pseudodisaccharide substrates on the l-amino group only, it is necessary to block the 6'-amino group with a suitable amino protecting group as defined above.. This protecting group can then be removed after the l-amino group is substituted to yield the desired aminoglycoside derivative. An example of a 6'-N protection + 1-N acylation 6'-N deprotection procedure is given in Example 4 of the Experimental Section. The first step of the instant process involves forming the zinc salt:aminoglycoside complex. This step is accomplished by mixing the aminoglycoside substrate compound and the zinc salt in water. Thorough mixing of these two components in the water is crucial and can be accomplished by employing methods such as sonication, vigorous stirring and mixing, moderate warming, etc. An aqueous solution may not always be obtained, but a thoroughly mixed or dispersed suspension is sufficient. Between a 2:1 and a 6:1 molar ratio of zinc salt:aminoglycoside substrate can be used, with the usual ratio employed being about 4:1. The second step of the instant process entails diluting the above mixture with a polar, organic solvent. By the term "polar, organic solvent" we mean a solvent such as formamide, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, hexamethyl phosphoramide, t-butanol, tetrahydrofuran, acetonitrile, acetone, sulfolane, tetramethylurea, 2-pyrrolidinone, N-methylpyrrolidinone, dioxane, and the like, or a mixture of any of the above solvents, with the preferred solvent for acylation being dimethylformamide. The preferred "polar, organic solvent" for alkylation is also dimethylformamide. Between 2 and 10 volumes of polar, organic solvent per volume of water can be used, with the usual amount employed being about 5 volumes of organic solvent per volume of water. The resultant mixture is usually stirred for about five minutes to about one hour before proceeding to the next step. The mixture can be either heterogeneous or homogeneous at this point. The final step of the instant process involves adding the appropriate acylating or alkylating agent to the mixture of step 2 of the process. The amount of acylating agent that can be used ranges from a 1:1 to a 2:1 ratio of acylating agent:aminoglycoside, with about a 1.5:1 ratio being typical. A ratio range of 5:1 to 20:1 of alkylating agent:aminoglycoside can be used, with the usual ratio employed being about 10:1. The time required to complete the acylation in the final step is not critical. The acylation reaction can be stirred for a period ranging between 0.5 and 24 hours, with the usual time being about 15 hours. The alkylation reaction requires longer periods of time, encompassing a range of fifteen hours to sixty hours. The temperature at which the instant process is carried out can range from between OOC. and 400C. Typically the reaction is run between 200C. and 250C. Once the acylation reaction of the final step is completed, recognized isolation procedures may be utilized to obtain the desired purified product. One such procedure involves column chromatography on either silica gel or Chelex 100 resin (BioRad Labs, 2200 wight Ave., Richmond, Ca. 94804) to adsorb the zinc salt from and separate the desired product from the resultant product mixture obtained from the instant process. To carry out this procedure, the solvent is evaporated in vacuo from the product mixture. The resultant residue is dissolved or suspended in an appropriate solvent and then loaded onto a column packed with the same solvent, using either Chelex 100 resin or silica gel as the stationary phase. An example of an appropriate solvent for use with the Chelex 100 resin is aqueous ammonium hydroxide. An example of an appropriate solvent for use with the silica gel is a 30:10:1 volume ratio of dichloromethane:methanol:ammonium hydroxide. The column is then eluted by common procedures (gradient or stepwise). The product can be chromatographed again on a second column of ion-exchange resin (e.g. Bio-Rex 70) or silica gel if necessary. Chelex 100 is a styrene divinylbenzene copolymer functionalized with iminodiacetate ions which act as chelating ligands for binding metal ions. Another isolation procedure removes Zn(II) ions by precipitation as the insoluble sulfide salts followed by ion-exchange chromatography to separate the desired products. Specifically, this procedure involves a) evaporating the solvent from the product mixture, dissolving the resultant residue in dilute acid (e.g., 0.1N HC1) and bubbling hydrogen sulfide gas through this mixture until precipitation of the zinc ion is complete, and b) chromatographing the filtered solution from step a) on an ion-exchange resin such as Bio-Rex 70 (BioRad Labs, supra) using an ammonium hydroxide solution or gradient to elute the desired product. Bio-Rex 70 is a weakly acidic cation exchange resin containing carboxylic acid groups on a macroreticular acrylic polymer lattice. Similar useful ion-exchange resins are Amberlite CG-50 and Amberlite IRC-50 (Rohm and Haas Co., Philadelphia, Pa.) The l-N-acylated and l-N-alkylated aminoglycosides made by the process of this invention, some of which having the 1-N substituent in the protected amino or N-protected form, and in addition some of the various pseudodisaccharides having the 6'-amino group protected, are intermediates to their respective deprotected amino or N-deprotected compounds. These l-(protected amino and N-protected) acyl derivatives can be converted to their corresponding deprotected forms according to procedures well recognized in the art. These deprotected compounds, either in their free base form or their pharmaceutically acceptable acid addition salt form, are useful for treating infections in warm-blooded animals caused by gram-positive and gram-negative bacteria. These compounds can be administered parenterally, in the free base form or in the pharmaceutically acceptable acid addition salt form, using pharmaceutically acceptable formulations known in the art. These compounds can also be administered as veterinary compositions, such as, for example, in the feed or drinking water of farm animals to treat infections such as colibacillosis or swine dysentery. Alternatively, these compounds can be used as a surface disinfectant. Solutions containing as little as 0.01 percent by weight of the antibiotic are effective for disinfecting purposes. Such solutions, preferably also containing a detergent or other cleansing agent, are useful for disinfecting objects such as glassware, dental and surgical instruments, and surfaces such as walls, floors, and tables in areas where maintenance of sterile conditions is important, i.e. hospitals, food-preparation areas, and the like. The antibacterial activity of the deprotected compounds of this invention is illustrated by the following in vitro and in vivo test data obtained with representative compounds. In Table I, the minimum inhibitory concentration (MIC) for representative compounds against a wide range of gram-positive and gram-negative bacteria is presented. The MIC values were obtained by the standard agar dilution test. Table 1 Antibiotic Activity of 1-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Staphylococcus aureus X1.1 8 8 64 8 8 Staphylococcus aureus V41 16 16 128 8 8 Staphylococcus aureus X400 32 64 > 128 64 16 Staphylococcus aureus S13E 16 16 64 8 8 Staphylococcus epidermidis EPI1 8 8 64 8 8 Staphylococcus epidermidis EPI2 4 4 64 4 4 Streptococcus pyogenes C203 64 64 > 128 16 16 Streptococcus pneumoniae Park I 64 64 > 128 16 16 Streptococcus group D X66 > 128 > 128 > 128 > 128 > 128 Streptococcus group D 9960 > 128 > 128 > 128 > 128 > 128 Hemophilus influenzae C.L. 16 32 64 16 16 Hemophilus influenzae 76 16 32 64 16 16 Shigella sonnei N9 16 16 128 16 16 Escherichia coli N10 8 16 > 128 8 16 Escherichia coli EC14 16 16 128 16 32 Table 1 cont. Antibiotic Activity of 1-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Escherichia coli TEM 4 4 32 2 8 Klebsiella pneumoniae X26 8 8 64 4 8 Klebsiella pneumoniae KAE 16 16 > 128 8 16 Enterobacter aerogenes X68 8 16 64 8 16 Enterobacter aerogenes C32 8 8 64 8 8 Enterobacter aerogenes EB17 16 16 64 8 16 Enterobacter cloacae EB5 16 16 128 16 32 Enterobacter cloacae 265A 32 32 128 32 32 Salmonella heidelberg X514 16 16 128 16 32 Salmonella typhimurium 1335 16 16 128 8 32 Pseudomonas aeruginosa X528 64 64 > 128 64 16 Pseudomonas aeruginosa X239 64 64 > 128 32 8 Pseudomonas aeruginosa Ps18 128 128 > 128 32 32 Table 1 cont. Antibiotic Activity of 1-N-Substituted Derivatives of Apramycin and Nebramine vs. Gram-Positive and Gram-Negative Bacteria Test CompoundÚ Test Organism* Minimum Inhibitory Concentration (mcg/ml) 1 2 3 4 5 Serratia marcescens X99 8 16 64 8 64 Serratia marcescens SE3 16 16 128 16 > 128 Proteus morganii PR15 128 128 > 128 32 32 Proteus inconstans PR33 8 8 128 8 128 Proteus rettgeri PR7 8 4 > 128 8 8 Proteus rettgeri C24 8 8 64 8 64 Citrobacter freundii CF17 16 16 > 128 16 16 Bordetella bronchiseptica 16 64 64 > 128 32 64 #Numerals and letters following the names of test microorganisms refer to the strains. 1Test compounds numbered 1-5 are as follows: 1 = 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin 2 = 1-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin (racemic side chain) 3 = 1-N-glycylapramycin 4 = 1-N-ethylapramycin 5 = 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) nebramine Table II illustrates the effective dose (ED50, the effective dose to protect 50 percent of the test animals) for representative compounds of this invention against an experimental bacterial infection in mice. Table II Subcutaneous Therapy of Staphylococcus aureus 3055 Infections in Mice Test Compound Effective Dose (ED50) l-N-(S-y-amino-a-hydroxybutyryl)- apramycin < 4.351 1-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl)- apramycin4 7.1ê l-N-glycylapramycin 59.53 l-N-ethylapramycin 4.5 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) nebramine 8.75 mg/kg x 2 subcutaneous doses at 1 and 5 hours post infection 1. mice were challenged with 68.1 LD50ls. 2. mice were challenged with 331 LD50's. 3. mice were challenged with 1,794 LD50's. 4. mixture of diastereomers The ED50 values were determined as described by W.E. Wick et al., Journal of Bacteriology, 81 [No. 2], 233-235 (1961). Table III, shown below, illustrates the in vitro activity of l-N-(S-y-amino-a-hydroxybutyryl)apramycin, l-N-(RS-y-amino-a-hydroxybutyryl)apramycin, and l-N-(S-y-amino-a-hydroxybutyryl)nebramine against an apramycin resistant strain of E. coli. The results below were obtained using the standard disc-plate method. The numerical values are the diameters in millimeters of the zones of inhibition observed against this bacterium. Compounds substituted with the y-amino- a-hydroxybutyryl and analogous side chains are useful in treating infections caused by apramycin resistant bacteria such as the one above. The test organism (E. coli X563) used in the experiments summarized in Table III is. resistant to apramycin due to a 3-N-acetyltransferase which enzymatically inactivates apramycin. Table III Antibiotic Activity of 1-N-Substituted Derivatives of Apramycin and Nebramine Against Apramycin-Resistant Bacteria Zone of InhibitionÚ (diameter in mm) Test Organism Test Compound No.ê 1 2 3 4 5 Escherichia coli X563 12.4 12.5 Tr NA4 11.3 14.7 13.6 9.4 NA4 12.1 1. Concentration is 15 mcg/disc, and zone of inhibition values are given for each of the two test runs performed for each compound. 2. Test compounds numbered 1-5 are as follows: 1 = 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin 2 = 1-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin (racemic side chain) 3 = 1-N-glycylapramycin 4 = 1-N-ethylapramycin 5 = 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) nebramine 3. Tr = Trace zone of activity ( < 8 mm) around the disc (6.35 mm diameter) 4. NA = not active The following Examples (1-8) are provided to further illustrate this invention. Preparations 1 and 2 provide examples of methods for synthesizing activated esters used in the process of the present invention. It is not intended that this invention be limited in scope by reason of any of the preparations or examples. In the following preparations and examples, carbon-13 nuclear magnetic resonance spectra, field desorption mass spectra and optical rotations are abbreviated c.m.r., f.d.m.s., and o.r., respectively. The nuclear magnetic resonance spectra were obtained on either a Varian Associates FT-80 Spectrometer or a JEOL JNM-PS-100 Spectrometer using dioxane as the internal standard. The chemical shifts are expressed in 6 values (parts per million downfield from tetramethylsilane). The field desorption mass spectra were taken on a Varian-MAT 731 Spectrometer using carbon dendrite emitters. Optical rotations were measured on a Perkin Elmer Model 241 Polarimeter. Thin layer chromatography was carried out.on E. Merck silica gel plates. Preparation 1 Preparation of N-(S-y-Benzyloxycarbonylamino-ahydroxybutyryloxy)succinimide S-y-Benzyloxycarbonylamino-a-hydroxybutyric acid (10.1 g, 40 mmol) and N-hydroxysuccinimide (4.60 g., 40 mmol) were dissolved in ethyl acetate (220 ml.) and cooled in an ice bath. N,N'-Dicyclohexylcarbodiimide (8.25 g., 40 mmol) was added and the mixture was stirred overnight. The resulting white precipitate was filtered, and the filtrate was evaporated to dryness under reduced pressure. The residue was triturated with pentane (200 ml.). The resultant white solid was filtered, washed with pentane and air-dried to yield 9.37 g (67%) of product. Crystallization from ethyl acetate yielded purified material, m.p. 108"C. Preparation 2 Preparation of N-(N-Benzyloxycarbonylglycyloxy) succ inimide N-Benzyloxycarbonylglycine (10.9 g., 52.2 mmol) and N-hydroxysuccinimide (6.0 g., 52.2 mmol), were dissolved in dioxane (100 ml.) and cooled in a cold water bath. N,N'-Dicyclohexylcarbodiimide (10.8 g., 52.2 mmol) was added, and the mixture was stirred overnight. The white precipitate which formed was filtered, washed and air-dried (11.8 g., 100% of dicyclohexylurea). The filtrate was evaporated to dryness under reduced pressure, and the residual solid (16.2 g.) was crystallized from ethyl acetate (50 ml.) to give 8.4 g. (53%) of crystalline N-(N-benzyloxycarbonylglycyloxy)succinimide, m.p. 110.5-112"C. Example 1 Preparation of 1-N- (S-y-Amino-a-hydroxybutyryl) apramycin A solution of apramycin (3.07 g, 5.7 mmol) in water (40 ml) was treated with zinc (II) acetate dihydrate (5.0 g, 22.8 mmol) and this mixture was diluted with dimethylformamide (200 ml). The resultant white opaque mixture was stirred for one hour and treated with N- (S-y-benzyloxycarbonylamino-a-hydroxy- butyryloxy)succinimide (2.86 g, 8.2 mmol), slowly yielding a homogeneous solution. After stirring overnight at ambient temperature, the solvent was evaporated under reduced pressure. The resultant residue was dissolved in a 10:10:1 mixture of dichloromethane: methanol:concentrated ammonium hydroxide and loaded on a silica gel column (Grace 950, 500 g) prewashed with the same solvent mixture. This column was eluted stepwise with two different mixtures of dichloromethane: methanol:ammonium hydroxide (10:10:1, then 3:3:1). Fractions containing the desired product were found by thin layer chromatography, combined, concentrated and lyophilized, yielding a glassy residue (1.7 g) contraining 1-N-(S-y-benzyloxycarbonylamino-y-hydroxy- butyryloxy)apramycin. This residue was dissolved in a solution of 25% aqueous dioxane (50 ml) and glacial acetic acid (1 ml), treated with 5% palladium on carbon (700 mg), and hydrogenated at 4 atmospheres for 24 hours at room temperature. The palladium catalyst was removed by filtration and the filtrate was evaporated to dryness. The resultant residue was loaded on a column of 115 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 9.5). This column was eluted with a linear gradient of 0.01N ammonium hydroxide (2 1) and 0.4N ammonium hydroxide (2 1). The fractions collected from this column were analyzed by thin layer chromatography and by their antimicrobial activity against the apramycinresistant E. coli X563. The two fractions active against the above bacterium were combined and lyophilized to yield l-N-(S-y-amino-a-hydroxybutyryl)apramycin (200 mg). Thin layer chromatography Rf was 0.14 using a 3:1:2 volume ratio of methanol:methylene chloride: concentrated ammonium hydroxide. Example 2 Preparation of l-N-(RS-y-Amino-a-hydroxybutyryl)- apramycin The same procedure as described in Example 1 was followed except the acylating agent employed was N-(RS-y-benzyloxycarbonylamino-a-hydroxybutyrylOxy)suc- cinimide (racemic mixture). The product mixture of diastereomers, l-N-(RS-y-amino-a-hydroxybutyryl)apramycin, was not resolved by chromatography, and exhibited the following physical chemical properties: c.m.r. (D2O-H2O, pH > 10) 6 33.0 (2x), 35.0, 35.6, 37.8, 49.9, 50.2, 50.5, 53.3, 61.8, 62.4, 66.4, 67.9, 70.7, 71.0, 71.8, 73.6, 74.2, 75.3, 77.1, 87.2, 95.3, 96.5, 101.6, 177.1 ; f.d.m.s. m/e = 641 (P+1) ; o.r. [&alpha;]D25 + 119.9 (H2O, C = 6 mg/ml). Example 3 Preparation of l-N-Glycylapramycin Apramycin (2.16 g, 4 mmol) was dissolved in water (40 ml) and treated with zinc(II) acetate dihydrate (3.5 g, 16 mmol). The resulting white mixture was diluted with dimethylformamide (200 ml) and treated with N- (N-benzyloxycarbonylglycyloxy) succinimide (1.84 g, 6 mmol). After the solution was stirred overnight at room temperature, the solvent was evaporated under reduced pressure. The residue thus obtained was dissolved in 0.1N hydrochloric acid (250 ml). This solution was treated for one hour with a slow stream of gaseous hydrogen sulfide, yielding a white precipitate which was stirred overnight and then was filtered. The filtrate was evaporated to dryness, and the residue was dissolved in 1N sodium hydroxide (50 ml, solution pH 9.4). The solution was loaded on a column of 300 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 10, prewashed with 0.01N ammonium hydroxide). After loading, the column was eluted with 400 ml of 0.01N ammonium hydroxide, followed by a linear gradient of 0.01N ammonium hydroxide (2 1) and 0.05N ammonium hydroxide (2 1). l-N-(N- benzyloxycarbonylglycyl)apramycin was readily eluted from the column, giving 0.61 g (21%) of relatively pure product. Later fractions contained smaller amounts of the 2'-N-substituted and 3-N-substituted isomers. l-N-(N-Benzyloxycarbonylglycyl)apramycin (0.51 g, 0.70 mmol) was dissolved in a mixture of ethanol (100 ml), water (16 ml) and 1N hydrochloric acid (4.2 ml) and hydrogenated over 5% palladium on charcoal (2 g)- at 60 psi overnight at room temperature. The catalyst was filtered and the filtrate was evaporated under reduced pressure. The residue thus obtained was dissolved in water, the pH of the solution was adjusted to 8 with 1N sodium hydroxide, and the resulting mixture was loaded on a column of 100 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 10, prewashed with 0.01N- ammonium hydroxide). After loading, a linear gradient was begun with 0.01N ammonium hydroxide (1 1) and 0.1N ammonium hydroxide (1 1). The fractions containing product were found by thin layer chromatographic analysis, were combined and lyophilized to yield 258 mg (62%) of l-N-glycylapramycin: c.m.r. (D2O-H2O, pH > 10) 6 32.9 (2x), 34.9, 44.8, 49.7, 50.1, 50.5, 53.2, 61.7, 62.3, 66.2, 68.0, 71.0, 71.8, 73.5, 74.2, 75.4, 76.9, 87.2, 95.5, 96.5, 101.6, 176.4; f.d.m.s. m/e 597(P+1); thin layer chromatography Rf= 0.31 using a 3:1:2 volume ratio of methanol methylene chloride: concentrated ammonium hydroxide. Example 4 A. Preparation of 6'-N-Benzyloxycarbonylnebramine Nebramine (15.3 g, 50 mmol) was dissolved in water (400 ml) and tetrahydrofuran (150 ml). A solution of benzyloxycarbonylimidazole (11.6 g, 57 mmol) in tetrahydrofuran (300 ml) was added dropwise over a 90-minute period, and the reaction mixture was stirred overnight at room temperature. Tetrahydrofuran was evaporated under reduced pressure, and the resulting aqueous solution was lyophilized. The crude product thus obtained was dissolved in water (100 ml) and loaded on a column of 1500 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 9.7). The column was eluted stepwise with 0.005N ammonium hydroxide (1.5 1), 0.015N ammonium hydroxide (2 1), 0.03N ammonium hydroxide (5 1) and 0.05N ammonium hydroxide (10 1). Benzyloxycarbonylnebramine was eluted with 0.05N ammonium hydroxide; fractions were analyzed by thin layer chromatography and appropriate fractions were combined and lyophilized to give 9.7 g (44%) of product. B. Preparation of l-N-(S-y-Amino-a-hydroxybutyryl)nebramine 6'-N-Benzyloxycarbonylnebramine (1.32 g, 3 mmol) was dissolved in water (20 ml) and treated with zinc(II) acetate dihydrate (2.63 g, 12 mmol). The mixture was diluted with dimethylformamide (100 ml), treated with N-(S-y-benzyloxycarbonylamino-a-hydroxybutyryloxy)succinimide (1.58 g, 4.5 mmol) and stirred overnight at room temperature. Solvent was evaporated under reduced pressure; the resulting residue was dissolved in methanol and re-evaporated to remove all dimethylformamide. The residue was dissolved in 30:10:1 dichloromethane:methanol:ammonium hydroxide and loaded on a column of 140 g Grace 950 silica gel packed in the same solvent. The column was eluted with 30:10:1 dichloromethane :methanol ammonium hydroxide. Appropriate fractions were combined and evaporated under reduced pressure. The resulting residue was dissolved in 1:1 dioxane:water, was filtered and lyophilized to give 1.29 g (64%) of crude 6'-N benzyloxycarbonyl-l-N- (S-y-benzyloxycarbonylamino- a-hydroxybutyryl) nebramine. 6 '-N-Benzyloxycarbonyl-l-N- (S-y-benzyloxy- carbonylamino-a-hydroxybutyryl)nebramine (1.10 g, 1.6 mmol) was dissolved in a mixture of dioxane (25 ml), water (25 ml) and glacial acetic acid (1 ml). 5% palladium on charcoal (280 mg) was added, and the mixture was hydrogenated at 42 psi overnight at room temperature. The catalyst was filtered and washed with hot water, and the filtrate was evaporated under reduced pressure. The residual oil was dissolved in water (20 ml) and loaded on a column of 70 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 10). The column was eluted with a gradient of 2 liters of 0.005N ammonium hydroxide and 3 liters of 0.5N ammonium hydroxide. Fractions were analyzed by thin layer chromatography, and the appropriate fractions were combined and lyophilized to yield 283 mg (43%) of l-N-(S-y-amino-ahydroxybutyryl)nebramine: c.m.r. (D2O-H2O, pH > 10) 6 33.2, 34.7, 35.4, 37.5, 42.1, 49.7, 50.1, 50.3, 67.0, 70.5, 73.2, 75.1, 77.0, 86.2, 100.1, 176.5; f.d.m.s. m/e 408(P+l); thin layer chromatography, Rf= 0.20 using a 3:1:2 volume ratio of methanol:methylene chloride:concentrated ammonium hydroxide. Example 5 Preparation of l-N-Ethylapramycin Apramycin (1.08 g., 2.0 mmol) and zinc (II) acetate dihydrate (1.76 g., 8.0 mmol) were added to water (20 ml) and this mixture was diluted with dimethylformamide (100 ml). The resultant white mixture was stirred for 1 hour and ethyl iodide (3.12 g., 20 mmol) was added. After stirring overnight at room temperature, solvent was evaporated under reduced pressure. The resultant residue was suspended in a 30:10:1 mixture of methanol:dichloromethane:concentrated ammonium hydroxide (25 ml) and loaded on a silica gel column (Grace 950, 100 g) prewashed with the same solvent mixture. The column was eluted stepwise first with a 30:10:1 mixture of methanol:dichloromethane: concentrated ammonium hydroxide (500 ml) and then with a 30:10:15 mixture of methanol:dichloromethane:concentrated ammonium hydroxide. The fractions containing the desired product were combined and evaporated to dryness (1.76 g). The resultant residue was dissolved in water (15 ml); the pH of this solution was adjusted to 8.8 and then loaded on a column of 300 ml Bio-Rex 70 (ammonium hydroxide cycle, pH 9.8, prewashed with 0.01N ammonium hydroxide (1 1.)). The column was eluted with a linear gradient of 0.01N ammonium hydroxide (2 1) and 0.1N ammonium hydroxide (2 1). Fractions were analyzed by thin layer chromatography and the fractions containing the desired product were combined and lyophilized to yield l-N-ethylapramycin (103 mg, 10% yield): c.m.r. (D2O-H2O, pH > 10) 6 14.9, 32.9, 33.1, 33.8, 41.2, 49.9, 50.4, 53.6, 57.5, 61.9, 62.5, 66.4, 68.0, 71.2, 72.0, 73.9, 74.4, 76.4, 77.3, 87.8, 95.8, 96.5, 101.6; f.d.m.s. m/e 568(P+1); thin layer chromatography, Rf = 0.29 using a 6:2:3 volume ratio of methanol:methylene chloride:concentrated ammonium hydroxide. Example 6 Preparation of l'-N-(n-Butyl)apramycin Method 1 Apramycin (54 mg, 0.1 mmol) was dissolved in water (1 ml). Zinc acetate dihydrate (100 mg, 0.4 mmol) was added to this solution and the mixture was briefly sonicated to disperse crystals of zinc acetate. The mixture was diluted with dimethylformamide (5 ml), 1iodo-n-butane (0.115 ml), 1 mmol) was added and this reaction mixture was stirred at room temperature for 4 duos. Thin layer chromatographic analysis (developed in 6:2:3 methanol:dichloromethane:ammonium hydroxide solution, visualized by chlorox spray and heating followed by starch-iodine spray reagent) of the reaction mixture showed a mixture of l-N-(n-butyl)apramycin (ref=0.52) and 3-N-n-butyl)apramycin (R=0.59) as major products along with unreacted apramycin (Rf=0.18). Bioautography of'another thin layer chromatography plate against B. subtilis X12 showed a good zone of inhibition due to l-N-(n-butyl)apramycin but not for 3-N-(n-butyl) apramycin. Method 2 The reaction of Method 1 was repeated using l-bromo-(n-butane) (0.11 ml, 1 mmol) instead of 1 iodo- (n-butane). This reaction appeared to give a lower yield of the desired l-N-(n-butyl)apramycin than did the reaction of Method 1. Example 7 Preparation of l-N-Benzylapramycin The reaction of Example 6, Method 1, was followed, except that benzyl bromide (0.06 ml, 0.5 mmol) was used instead of l-iodo-n-butane. The reaction mixture was stirred at room temperature for 19 h, at which time thin layer chromatographic analysis showed l-N-benzylapramycin (Rf=O .54) to be the major mono-alkyl product. Bioautography of another thin layer chromatography plate against B. subtilis X12 showed a zone of inhibition due to l-N-benzylapramycin. Example 8 Preparation of l-N-Allylapramycin Method 1 The reaction of Example 6, Method 1, was followed that allyl bromide (0.043 ml, 0.5 mmol) was used instead of 1-iodo-n-butane. The reaction mixture was stirred at room temperature for 22 h, at which time thin layer chromatographic analysis showed 1-Nallylapramycin (Rf = 0.45) was present as the principal mono-alkylated product. Bioautography of a second thin layer chromatography plate against B. subtilis X12 showed a good zone of inhibition due to l-N-allyl apramyc in. Method 2 The reaction of Method 1 above was repeated, except that allyl iodide (0.05 ml, 0.55 mmol) was used instead of allyl bromide. The reaction mixture was stirred at room temperature for 22 h, when thin layer chromatographic analysis of the reaction mixture gave the same results as in Method 1.

Claims: Claims 1. A l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2-deoxystreptz;mine aminoglycoside selected from the group consisting of; apramycin, 3' -hydroxyapramycin, a or -(C1 to C6 alkyl)aprosaminide, a or B-(C2 to C6 hydroxyalkyl)aprosaminide, a or ss-(C2 to C6 aminoalkyl)aprosaminide, a or h-(C2 to C6 protected aminoalkyl)apros aminide, a or D-(C3 to C6 aminohydroxyalkyl) aprosaminide, a or D-(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmaceutically-acceptable acid addition salt thereof; wherein the acyl or alkyl substituent group is selected from the group consisting of y-amino-a-hydroxybutyryl, y-protected amino a-hydroxybutyryl, B-amino-a-hydroxypropionyl, D-protected amino-a-hydroxypropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3 carbonyl, N-protected 3-hydroxypyrrolidine 3-carbonyl, 3-hydroxyazetidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl) acetyl, N-pro tected 2-hydroxy-2-(azetidin-3-yl)acetyl, 4-amino-2-hydroxybutyl, 3-amino-2-hydroxy propyl, 2-aminoethyl, 3-hydroxypyrrolidine 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl)ethyl, C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl. 2. A l-N-acylated or l-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside as claimed in claim 1 selected from : 1-N-(S-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin, 1-N-(RS-&gamma;-amino-&alpha;-hydroxybutyryl) apramycin (racemic side chain), l-N-glycylapramycin, l-N-ethylapramycin, l-N-allylapramycin, l-N-benzylapramycin, and l-N-(n-butyl)apramycin. 3. A l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside as claimed in claim 1 selected from the group which includes the above compounds excluding those bearing amino-protected or N-protected substituents. 4. A pharmaceutical formulation which comprises as an active ingredient a l-N-acylated or a 1-Nalkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside as claimed in claim 3 associated with at least one carrier or diluent therefor. 5. A veterinary formulation which comprises as an active ingredient a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2-deoxystreptamine aminoglycoside as claimed in claim 3 associated with at least one carrier or diluent therefor. 6. A surface disinfectant formulation which comprises as an active ingredient a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2deoxystreptamine aminoglycoside as claimed in claim 3 associated with at least one carrier or diluent therefor. 7. A process for preparing a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted 2deoxystreptamine aminoglycoside as defined in claim 1 or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2ai and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: : apramycin, 3'-hydroxyapramycin, a or -(C1 to C6 alkyl)aprosaminide, a or -(C2 to C6 hydroxyalkyl)aprosaminide, a or D-(C2 to C6 protected aminoalkyl) aprosaminide, a or R-(C3 to C6 (pro- tected amino) hydroxyalkyl) aprosaminide, lividamine, paromamine and the 6'-N protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2; with between two and six molar equivalents of a zinc salt of the formula: : Zn(X)2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then carrying out the reaction at a temperature between OOC. and 400C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used is a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, ss-protected aminoa-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used are C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C5 allyl bromide, C3 to C allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; and (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. 8. A process as claimed in claim 6 wherein the product is selected from: l-N-(S-y-amino-a-hydroxybutyryl)apramycin, l-N-(RS-y-amino-a-hydroxybutyryl)apramycin (racemic side chain), l-N-glycylapramycin, l-N-ethylapramycin, l-N-butylapramycin l-N-benzylapramycin l-N-allylapramycin, and l-N-(S-y-amino-a-hydroxybutyryl)nebramine. 9. A l-N-acylated or a l-N-alkylated derivative of a 4-0-substituted-2-deoxystreptamine aminoglycoside as defined in claim 3 for use in treating warm-blooded animals having a bacterial infection. 10. A l-N-acylated or a l-N-alkylated derivative of a 4-0-substituted-2-deoxystreptamine aminoglycoside as defined in claim 3 for use as a surface disinfectant. Claims 1. A process for preparing a l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted-2deoxystreptamine aminoglycoside selected from the group consisting of; apramycin, 31 -hydroxyapramycin, a or ss-(C1 to C6 alkyl)aprosaminide, a or ss-(C2 to C6 hydroxyalkyl)aprosaminide, a or ss-(C2 to C6 aminoalkyl)aprosaminide, a or D-(C2 to C6 protected aminoalkyl)apros aminide, &alpha; or ss-(C3 to C6 aminohydroxyalkyl) aprosaminide, a or ss-(C3 to C6 (protected amino) hydroxyalkyl) aprosaminide, or a pharmaceutically-acceptable acid addition salt thereof; wherein the acyl or alkyl substituent group is selected from the group consisting of y-amino-a-hydroxybutyryl, y-protected amino a-hydroxybutyryl, -amino-a-hydroxypropionyl, ss-protected amino-&alpha;-hydroxypropionyl, glycyl, N-protected glycyl, 3-hydroxypyrrolidine-3- carbonyl, N-protected 3-hydroxypyrrolidine 3-carbonyl, 3-hydroxyazetidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl, 2-hydroxy-2-(azetidin-3-yl)acetyl, N-pro tected 2-hydroxy-2-(azetidin-3-yl) acetyl, 4-amino-2-hydroxybutyl, 3-ari'ino-2-hydroxy- propyl, 2-aminoethyl, 3-hydroxypyrrolidine 3-methylene, 3-hydroxyazetidine-3-methylene, 2-hydroxy-2-(azetidin-3-yl)ethyl, C2 to C4 alkyl, C3 to C5 allyl, benzyl and substituted benzyl; or any one of the group lividamine, paromamine and the 6'-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2a, and gentamine C2b, or a pharmaceutically-acceptable acid addition salt thereof which comprises the steps of: (1) combining an aminoglycoside substrate selected from the group consisting of: : apramycin, 3'-hydroxyapramycin, a or D-(C1 to C6 alkyl)aprosaminide, a or 5-(C2 to C6 hydroxyalkyl)aprosaminide, a or 5-(C2 to C6 protected aminoalkyl) aprosaminide, a or ss-(C3 to C6 (pro tected amino) hydroxyalkyl) aprosaminide, lividamine, paromamine and the 6'-N protected derivatives of nebramine, neamine, gentamine C1, gentamine Clal gentamine C2, gentamine C2a, and gentamine C2b; with between two and six molar equivalents of a zinc salt of the formula: Zn(X)2 in water; (2) diluting the above aqueous mixture with between two and ten volumes of a polar organic solvent, and; (3) adding between one and two molar equivalents of an acylating agent or between five and twenty molar equivalents of an alkylating agent to the above mixture of step (2) then carrying out the reaction at a temperature between OOC. and 400C. for a period between 0.5 and 24 hours for acylation or between 15 hours and 60 hours for alkylation, wherein: X is C1 to C10 carboxylate; the acylating agent used is a reactive derivative of an acyl moiety selected from the group consisting of y-protected amino-a-hydroxybutyryl, D-protected aminoa-hydroxypropionyl, N-protected glycyl, N-protected 3-hydroxypyrrolidine-3-carbonyl, N-protected 3-hydroxyazetidine-3-carbonyl and N-protected 2-hydroxy-2 (azetidin-3-yl) acetyl; and the alkylating agents used are C2 to C4 alkyl bromide, C2 to C4 alkyl iodide, C3 to C allyl bromide, C3 to C5 allyl iodide, benzyl bromide, benzyl iodide, substituted benzyl bromide and substituted benzyl iodide. (4) optionally, deprotecting protected amino or N-protected substituents; (5) optionally, reducing the carbonyl portion of an acyl substituent with a reducing agent; and (6) recovering as a free base or as a pharma ceutically-acceptable acid addition salt thereof. 2. A process of preparing 1-N-(S-y-amino- a-hydroxybutyryl)apramycin as claimed in claim 1 which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting the solution with dimethylformamide, treating with N-(S-y-benzyloxycarbonyl amino-a-hydroxybutyryloxy)succinimide overnight to form 1-N- (S-y-benzyloxycarbonylamino-a-hydroxybutyryl) - apramycin, followed by removal of the protecting agent and recovering a free base. 3. A process of preparing a racemic mixture of 1-N (RS-y-amino-a-hydroxybutyryl) apramycin which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting the solution with dimethylformamide, treating with racemic mixture of N (S-y-benzyloxycarbonylamino-a-hydroxybutyryloxy)succinimide overnight to form racemic mixture of 1-N (S -y-benzyloxycarbonylamino -a-hydroxybu tyryl) apramyc in, followed by removal of the protecting agent and recovering a free base. 4. A process of preparing l-N-glycyl apramycin as claimed in claim 1 which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting the solution with dimethylformamide, treating with N- (N- benzyloxycarbonylglycyloxy) succinimite overnight to form l-N-(N-benzyloxycarbonylglycyl)apramycin, followed by removal of the protecting agent and recovering as a free base. 5. A process of preparing l-N-(S-y-aminoa-hydroxy-butyryl)nebramine as claimed in claim 1 which comprises reacting 6'-N-benzyloxycarbonyl nebramine and zinc II acetate dihydrate in water, diluting with dimethylformamide, treating with N-(S-y-benzyloxy carbonyl-amino-a-hydroxybutyryloxy)succinimide overnight to give 6' -N-benzyloxycarbonyl-l-N-S-y-benzyloxy- carbonylamino-a-hydroxy butyryl)nebramine, followed by removal of the protecting agent. 6. A process of preparing l-N-ethylapramycin as claimed in claim 1 which comprises reacting apramycin and zinc II acetate dihydrate in water, diluting with dimethylformamide, and treating with ethyl iodide overnight and recovering the free base. 7. A process of preparing l-N-(n-butyl)- apramycin as claimed in claim 1 which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting in dimethylforman-ide, treating with l-iodon-butane for 4 days, and recovering as the free base. 8. A process of preparing l-N-benzylapramycin as claimed in claim 1 which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting in dimethylformamide, treating with benzyl bromide for 19 hours, and recovering as a free base 9. A process for the preparation of 1-Nallylapramycin as claimed in claim 1 which comprises reacting apramycin with zinc II acetate dihydrate in water, diluting with dimethylformamide, treating with allyl bromide for 22 hours, and recovering as a free base. 10. A l-N-acylated or a l-N-alkylated derivative of a 4-O-substituted 2-deoxystreptamine aminoglycoside as defined in claim 1 or any one of the group lividamine, paromamine and the 61-N-protected derivatives of nebramine, neamine, gentamine C1, gentamine Cla, gentamine C2, gentamine C2al and gentamine C2b or a pharmaceutically-acceptable acid addition salt thereof whenever prepared by the process as claimed in claim 1.

Inventors: KIRST, HERBERT ANDREW, TRUEDELL, BRENDA ALETHIA
Applicants: ELI LILLY AND COMPANY
Application year: 1981
Main classification: C07H15
IPC codes: C07H15, A61P31, C07D207, A61K31