CEPHEM COMPOUNDS, PROCESSES FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

Abstract: A new cephem compound of the formula: wherein R¹ is amino or a substituted amino group; R² is carboxy or a protected carboxy group; R is a group of the formula: -A₁-R 3a [in which A₁ is hydroxy(lower)alkylene, amino(lower)alkylene, protected amino(lower)alkylene, lower alkenylene or hydroxyimino(lower)alkylene wherein the hyrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R 3a is carboxy or a protected carboxy group] or a group of the formula: [in which A₂ is lower alkylene which may have an oxo group; and R 3b is organic group which may have suitable substituent(s)]; and Q is hydrogen or lower alkoxy; and pharmaceutically acceptable salts thereof and processes for their preparation and also a pharmaceutically composition comprising, as an effective ingredient, the above compound in association with pharmaceutically acceptable carriers. The invention also relates to the starting compounds and their preparation.

Description: A NEW CEPHEM COMPOUND, PROCESSES FOR THEIR PREPARATION, PHARMACEUTICAL COMPOSITIONS CONTAINING THEM AND THEIR STARTING COMPOUNDS The present invention relates to new cephem compounds and pharmaceutically acceptable salts thereof. More particularly, it relates to new cephem-compounds and pharmaceutically acceptable salts thereof, which have antimicrobial activities and to processes for preparation thereof, to pharmaceutical composition comprising the same, and to amcthod of using the same therapeutically in treatment of infectious diseases in human being and animals. Accordingly, it is one object of the present invention to provide new cephem compounds and pharm2- ceutically acceptable salts thereof, which are active against a number of pathogenic microorganisms Another object of the present invention is to provide processes for the preparation of new cephem compounds and pharmaceutically acceptable salts thereof. A further object of the present invention is to provide pharmaceutical composition comprising, as active ingredients, said new cephem compounds and pharmaceutically acceptable salts thereof. Still further object of the present invention is to provide a method or the treatment of infectious diseases caused by pathogenic bacteria in human being and animals. The object new cephem compounds are novel and can be represented by the following general formula (I). EMI2.1 wherein R, is amino or a substituted arino group; Rê is carboxy or a protected carboxy group; R is a group of the formula: -A1-R3a [in which A1 is hydroxy(lower)alkylene, amino(lower) alkylene, protected amino(lower)alkylene, lower alkenylene or hydroxyimino(lower)alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group@ and R3a is carboxy or a protected carboxy group] or a group of the formula: EMI2.2 [in which A2 is lower alkylene which may have an oxo group; and R3b is organicgroup which may have sui table substituent (s)]; and Q is hydrogen or lower alkoxy. As to the object compounds (I) and the starting compounds of the present invention, it is to be understood that there may be one or more stereoisomeric pair(s) such as optical and/or geometrical isomers due to zsymmetric carbon atom(s) and/or double bond(s), in the molecule, and these isomers are also included within the scope of the present invention. The particulars of such isomers will be made more clear in the following explanation. According to the present invention, the object compounds (I) can be prepared by the following processes which are illustrated in the following scheme. Process 1 EMI3.1 EMI3.2 or its reactive derivative at the mercapto group or EMI3.3 EMI3.4 or a salt thereof or a salt thereof Process 2 EMI3.5 Acryl awing EMI3.6 EMI3.7 or its reactive derivatives at the amino group or a salt thereof or a salt thereof Process 3 EMI4.1 Elimination of the amino protective EMI4.2 EMI4.3 or a salt thereof or a salt thereof Process 4 EMI4.4 Elimination of the amino protective EMI4.5 EMI4.6 or a salt thereof or a salt thereof wherein R1, R2, R and Q are each as defined above, Y is a group which can be substituted by a group of the formula: : EMI4.7 (wherein R is as defined above) Ria is acylamino; Rla is acylamino having a protected amino group; is is is acylamino having an amino group; - A1 is a protected amino(lower)alkylene; and A1" is amino(lower)alkylene. Among the starting compounds in the present invention, the compound (III) and a part of the acylating agent and t t?:e compounds (11) are novel and can be prepared by the Processes which are illustrated in the following scheme. Process A EMI5.1 or its reactive derivative at the mercapto group or its reactive derivative at the mercapto group or a salt thereof Process B 4 ii)H2N-OR"(VIII) EMI5.2 <tb> <SEP> i) <SEP> (optional <SEP> or <SEP> a <SEP> salt <tb> <SEP> HS- <SEP> 9 <SEP> 1 <SEP> i) <SEP> process) <SEP> thereof <SEP> HS./ <tb> <SEP> X-Co-R3 <SEP> elimination <SEP> 3a <tb> <SEP> of <SEP> or <SEP> the <tb> <SEP> C <SEP> carboxy <SEP> N <tb> <SEP> or <SEP> its <SEP> reactive-trotective <SEP> R4 <tb> derivative <SEP> zt <SEP> group <tb> <SEP> the <SEP> mercapto <SEP> on <SEP> (iota) <tb> group <SEP> or <SEP> a <SEP> salt <SEP> or <SEP> its <SEP> reactive <SEP> derivative <tb> <SEP> thereof <SEP> at <SEP> the <SEP> mercapto <SEP> group <SEP> or <SEP> a <tb> <SEP> salt <SEP> thereof <tb> <SEP> iz) <SEP> Reduction <SEP> i <SEP> HS <SEP> 45 <SEP> 1 <tb> <SEP> X-CH-Ria <tb> <SEP> (mb) <tb> or its reactive derivative at the mercapto group or a salt thereof Process C EMI6.1 or its reactive derivative at the mercapto group or z salt thereof or its reactive derivative at the mercapto group or a salt thereof or its reactive derivative at the mercapto group or a salt thereof Process D EMI6.2 or its reactive derivative at the mercapto group or its reactive derivative at the mercapto group or a salt thereof EMI6.3 <tb> Process <SEP> E <SEP> c <SEP> Ev'--, <tb> <SEP> 11 <SEP> COCOOH <tb> <SEP> Elimination <SEP> 6 <tb> <SEP> R8-0-R6 <SEP> of <SEP> the <SEP> amino <SEP> R6 <SEP> (In <tb> <SEP> protective <SEP> ( <SEP> or <SEP> a <SEP> salt <SEP> N <tb> <SEP> (xi) <SEP> group <SEP> > <SEP> thereof <SEP> R&num; ;\C-coo <tb> <SEP> \fI <tb> <SEP> i) <SEP> or <SEP> a <SEP> salt <SEP> ii) <SEP> N <tb> <SEP> thereof <SEP> s <SEP> 6 <tb> <SEP> O-R <tb> <SEP> (\tea) <tb> <SEP> Process <SEP> F <SEP> or <SEP> a <SEP> salt <SEP> thereto <tb> <SEP> 1 <SEP> Elimination <SEP> of <tb> <SEP> us <SEP> 3b <SEP> 8 <SEP> BI <SEP> 3b <SEP> the <SEP> amino-protective <tb> <SEP> ()Ct:TTT) <SEP> R <SEP> -A-N <SEP> N-R <SEP> D <SEP> croup <tb> <SEP> 2 <tb> <SEP> (X28.) <SEP> or <SEP> a <SEP> sat <SEP> . <SEP> thereof <tb> cr <SEP> a <SEP> salt <SEP> thereof <tb> EMI7.1 lower alkylating EMI7.2 or a salt thereof or a salt thereof EMI7.3 hydrazoic acid salt EMI7.4 or a salt thereof EMI7.5 or a salt thereof Process G EMI7.6 diazotization EMI7.7 or a salt thereof or a salt thereof EMI7.8 or its reactive derivative at the hydroxy group EMI8.1 or a salt thereof Elimination of the mercapto-protective EMI8.2 EMI8.3 or a salt thereof Process H EMI8.4 or a salt thereof or a salt thereof Process I EMI8.5 or its reactive derivative at the carboxy group or a salt thereof EMI8.6 or its reactive derivative at the amino group or a salt thereof EMI8.7 EMI8.8 or a salt thereof Process J EMI9.1 Acylating agent EMI9.2 EMI9.3 or its reactive derivative at the amino group or a salt thereof or a salt thereof Process K EMI9.4 Elimination of the carboxyprotective EMI9.5 EMI9.6 or a salt thereof or a salt thereof wherein RÚ, R1a, Rê, R3a, R3b, A2 and Y are each as defined above; X is lower alkylene; R4 is hydrogen or a lower aliphatic hydrocarbon group; 2 is a protected amino group; B is lower alkenylene; B is lower alkenylene; R5 is amino or a protected amino group; R6 is an organic residue which may have suitable substituent(s) R and R8 are each amino having a protectIve group; R2' is a protected carboxy group; is is lower alkoxy; R9 is lower alkyl; R10a is a mercapto-protective group; is lower alkylene; ; R11 and R12 are each an acid residue; and R1a''' is phenylacetamido having hydroxy and/or carboxy. Suitable pharmaceutically acceptable salts of the object compounds (I) are conventional non-toxic salt and include a metal salt such as an alkali metal salt (e.g. sodium salt, potassium salt, etc.) and an alkaline earth metal salt (e.g. calcium salt, magnesium salt, etc.), an ammonium salt, an organic base salt (e.g. trimethylamine salt, triethylamine salt, pyridine salt, picoline salt, dycyclohexylamine salt, N,N'-dibenzylethylenediamine salt, etc.), an organic acid salt (e.g. acetate, maleate, tartrate, methanesulfonate, benzenesultonate, formate, toluenesulfonate, etc.), an inorganic acid salt (e.g. hydrochloride, hydrobromide, sulfate, phosphate, etc.), or G salt with an amino acid (e.g. arginine, aspartic acid, glutamic acid, etc.), and the like. In the above and subsequent descriptions of the present specification, suitable examples and illustrations of the various definitions which the present invention include within the scope thereof are explained in details as follows. The term "lower" is intended to mean 1 to 6 carbon atoms, unless otherwise indicated. The term "halogen" includes fluorine, chlorine, bromine and iodine. Suitable "substituted amino group" for R1 may include an amino group substituted by a conventional substituent used in Cephalosporin and Penicillin compounds such as acyl as mentioned below, ar(lower)alkyl (e.g. benzyl, phenethyl, trityl, etc.) or the like. Suitable "protected amino" in the terms "protected amino" for R5 and Z, "acylamino having a protected amino group" for R1a' and "protected amino(lower)alkylene' for A and At may include an acylamino or an amino group substituted by a conventional protecting group such as ar(lower)alkyl which may have at least one suitable substituent(s), (e.g. benzyl, trityl, etc.) or the like. Suitable "acyl" and Itacyl moiety" in the term acylamino" may include carbamoyl, an aliphatic acyl group, an acyl group containing an aromatic ring (hereinafter referred to as aromatic acyl) and an acyl group containing a heterocyclic ring (hereinafter referred to as heterocyclic acyl). Suitable example of said acyl may be illustrated as follows: Aliphatic acyl such as lower or higher alkanoyl (e.g. formyl, acetyl, succinyl, hexanoyl, heptanoyl, stearoyl, etc.) lower or higher alkoxycarbonyl (e.g. methoxycarbonyl, ethoxycarbonyl, tert-butoxycarbonyl, tert-pentyloxycarbonyl, heptyloxycarbonyl, etc.); lower or higher alkanesulfonyl (e.g. methanesulfonyl, ethanesulfonyl, etc.); or the like. Aromatic acyl such as aroyl (e.g. benzoyl, toluoyl, naphthoyl, $etc.); ar(lower)alkanoyl (e.g. phenylacetyl, phenylpropionyl, etc.); aryloxycarbonyl (e.g. phenoxycarbonyl, naphthyloxycarbonyl, etc.); ar(lower)alkoxycarbonyl (e.g. benzyloxycarbonyl, phenethyloxycarbonyl, etc.); aryloxy(lower)alkanoyl (e.g. phenoxyacetyl, phenoxypropionyl, etc.); arylglyoxyloyl (e.g. phenylglyoxyoyl, naphthyl glyoxyloyl, etc.; arenesulfonyl (e.g. benzenesulfonyl, p-toluenesulfonyl, etc.); or the like; Heterocyclic acyl such as heterocycliccarbonyl (e.g. thenoyl, furoyl, nicotinoyl, etc.); heterocyclic(lower)alkanoyl (e.g. thienylacetyl, thiazolylacetyl, thiadiazolylacetyl, dithiinylacetyl, pyridylacetyl, pyrimidinylacetyl, triazolylacetyl, tetrazolylacetyl, furylacetyl, oxazolylacetyl, thiazolylpropionyl, etc.); heterocyclicglyoxyloyl (e.g. thiazolylglyoxyloyl, thienylglyoxyloyl, etc.); or the like. The acyl and acyl moiety as stated above may have one or more, same or different, suitable substituent(s) such as lower alkyl as mentioned below ; lower alkoxy (e.g. methoxy, ethoxy, propoxy, etc.); lower alkylthio (e.g. methylthio, ethylthio, etc.); lower alkylamino (e.g. methylamino, etc.); halogen (e.g. chlorine, bromine, fluorine or iodine); amino; a protected amino group as mentioned above:; hydroxy; a protected hydroxy such as tetrahydropyranyloxy or acyloxy wherein the acyl moiety is as stated above; imino; oxo; carboxy, protected carboxy as mentioned below; a group of the formula: =N-OR wherein R6 is as defined above; or the like. Preferable examples of the "acylamino" in the terms "acylamino" as the suitable substituted amino group for RÚ, "acylamino" for R1a, "acylamino having a protected amino group" for R1a' and "acylamino having an amino group" for R1a t±ày include phenylacetamido which may have hydroxy and/or carboxy ; and the group of the formula: EMI13.1 wherein R5 and R6 are each as defined above. In the connection, as we stated above, it is to be understood that the object compounds (I), (Ia), (Ic), (Id), (Ie) and (If) and the starting compounds (II),(IIa) (IIb), (IIc), (VIa), and (IX) include tautometric isomers. That is, in case that the group of the formula: EMI13.2 R5 is as defined above) is contained in the molecules of said object and starting compounds, said group of the formula can also be alternatively represented by its tautomeric formula EMI13.3 (R5 is imino or a protected imino group). That is, the both of said groups are in the state of equilibrium each other and such tautomerism can be represented by the following equilibrium. EMI13.4 wherein R5 and R5' are each as defined above. these types of tatutomerism between tghe amino-compound and the corresponding imino-compound as stated above have been well known in the literature, and it is obvious to a person skilled in the arts that both of the tautomeric isomers are easily convertible reciprocally and are included within the same category of the compound per Se. Accordingly, the both cf the tautomeric forms of the object compounds (I), (Ia), (Ic), (Id), (Ie) and (If) and the starting compounds (II), (IIb), (IIc), (VIa) and (IX) are clearly included within the scope of the present invention in the present specification and clains, the object and starting compounds include the grout of such tautomeric isomers are represented by using one of the exressions thereto, that is the formula defined above. EMI14.1 wherein R5 is as Furthermore, with regard to the object compounds (I), (Ia), (Ib), (Ic), (Id), (Ie) and (If) and the starting compounds (II), (IIa), (IIb), (IIc), (III), (IIIa), and (VIa), it is also to be noted that where the oxyimino groups is contained in the chemical structure thereof, and these compounds exist as geometrical isomers, i.e. syn isomer, anti isomer arid a mixture thereof. For examples with regard to the object compound (I), the syn isomer in the 7-position means one geometrical isomer having the partial structure represented by the following formula: EMI14.2 (wherein H and R6 are each as defined above) and the ant isomer in the 7-position means the other geometri- cal isomer having the partial structure represented by the following formula: EMI15.1 (wherein H5 and R are each as defined above). On the other hand, the syn isomer in the -position means one geometrical isomer having the partial structure represented by the following formula: EMI15.2 (wherein H R4 and X are each as defined above) and the anti isomer in the 5-position means one geometrical isomer having the partial structure represented by the following formula EMI15.3 (wherein R3a, R4 and X are each as defined above). Regarding the other object and starting compounds as mentioned above, the syn isomer and the anti isomer can also be referred to the same geometrical isomers as illustrated for the compound (I). In this regard, the above formula: EMI16.1 (wherein R32 R4, R5, R6 and X are each as defined above) are intended to represent both of the syn isomer and anti isomer as well as a mixture thereof and both of the respective isomers as well as a mixture thereof are included within the scope of the present invention. Concerning the starting thiol compounds (II1),(IIIa), (IIIb), (IIIc), (IIId), (IIIf), (IIIg), IIIh), (IIIi), (IV),(V),and (XXV), there are tautoeric isomers as snown by the following equilibrium: : EMI16.2 These types of tautomerism between l-substituted lc-tetrazole-5-thiol compounds and l-substituted 4,5dihydro -lH-tetrazole-5-thione compounds as stated above have ben well known in the arts, and it is obvious to a person skilled In the arts that both of the tautomeric isomers are equilibrated and lie in the reciprocally convertible state, and accordingly it is to be understood that such isomers are included within the same category of the compound per se. Accordingly, the both of the tautomeric forms are clearly included within the scope of the present invention. In the present specification, starting compounds including the group of such tautomeric isomers are represented by using one of the expressions therefor, i.e. 1-substituted 1H-tetrazole-5-thiol and the formula: EMI17.1 onlv for the convenient sake. Suitable 11organic residue which may have suitable substituent(s)' for R6 may include an aliphatic nd aromatic group, for example, lower alkyl (e.g. methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, neopentyl, tertpentyl, hexl, etc.); lower alkenyl (e.g. vinyl, 1-propenyl, allyl, 1-methylallyl, 1 or 2 or 3-butenyl, 1 or 2 or 3 or 4-pentenyl, 1 or 2 or 5 or 4 or 5-hexenyl, etc.); lower alkynyl (e.g. ethyl, 1-propynyl, 2propynyl, 1-methyl-2-propynyl, 1 or 2 or 3-butynyl, 1 or 2 or 3 cr 4-pentynyl, 1 or 2 or 3 or 4 or 5- hexynyl, etc.); ; cycloalkyl (e.g. cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, etc.); cyclo(lower)alkenyl (e.g. cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclohexenyl, etc.); aryl (e.g. phenyl, tolyl, xylyl, cumenyl, naphthyl, etc.); ar(lower)alkyl such as phenyl(lower)alkyl (e.g. benzyl, phenethyl, phenylpropyl, etc.); ar(lower)alkyl having at least one suitable sub stituent(s) such as halogen as mentioned above (e.g. chlorobenzyl, dichlorobenzyl, trichlorobenzyl, fluorobenzyl, fluorophenetyl, fluorophenylpropyl, etc.) or the like. Suitable protected carboxy forR2,R2'and R3a may include an esterified carboxy and-the like, and suitable examples of the ester moiety in said esterified carboxy may be the ones such as lower alkyl ester (e.g. methyl ester, ethyl. ester, propyl ester, isopropyl ester, butl ester, isobutyl ester, pentyl ester, hexyl ester, l-cyclopropyl- ethyl ester, etc.) which may have at least one suitable sustItuent(s), for example, lower alkanoyloxy(lower)alkyl ester (e.g. acetoxymethyl ester, propionyloxymethyl ester, butyryloxymethyl ester, valeryloxymethyl ester, pivaloyloxymethyl ester, 2-acetoxyethyl ester, 2propionyloxyethyl ester, hexanoyloxymethyl ester, etc.), lower alkanesulfonyl (lower) alkyl ester (e.g. 2-mesylethyl ester, etc. or mono(or di or tri)halo (lower) alkyl ester (e.g. 2-iodoethyl ester, 2,2,2trichloroethyl ester, etc.); lower alkenyl ester (e.g. vinyl ester, allyl ester, etc.); lower alkynyl ester (e.g. ethynyl ester, propynyl ester, etc.); ar(lovergalkyl ester which may have at least one suitable substituent(s) (e.g. benzyl ester, 4methoxybenzyl ester, 4-nitrobenzyl ester, phenethyl ester, trityl ester, diphenylmethyl ester, bis (methoxyphenyl)methyl ester, 3,4-dimethoxybenzyl ester, 4-hydroxy-3,5-ditertiarybutylbenzyl ester, etc.); aryl ester which may have at least one suitable sub stituent(s) (e.g. phenyl ester, 4-chlorophenyl ester, tolyl ester, tertiarybutylphenyl ester, xylyl ester, mesityl ester, cumenyl ester, etc.), and the like. Preferable examples of the esterified carboxy as mentioned above may include lower alkoxycarbonyl (e.g. methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, tert-butoxycarbonyl, pentyloxycarbonyl, tert pentyloxycarbonyl, hexyloxycarbonyl, l-cyclopropylethoxy- carbonyl, etc.) and phenyl(lower)alkcxycarbonyl (e.g. benzyloxycarbonyl, diphenylmethoxycarbonyl, etc.). Suitable "lover alkylene" in the terms "hydroxy- (lower)alkylene". "amino(lower)alkylene", "protected amino(lower)alkylene", "hydroxyimino(lower)alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group" for A1, a protected amino(lower)alkylene for A1', amino(lower) alkylene for A1", lower alkylene for X and A2', and lower alkylene which may have an oxo for A2 may include straight or branched one and may include methylene, ethylene, trimethylene, propylene, tetramethylene, pentamethylene, hexamethylene and the like. Suitable "lower aliphatic hydrocarbon group" in the terms "hydroxyimino(lower)alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group" for Aland "a lower aliphatic hydrocarbon group" for R may include a monovalent radical of a saturated or unsaturated, and straight or branched acyclic aliphatic hydrocarbon, and particularly may include lower alykl, lower alkenyl, lower alkynyl and the like, each of which is exemplified for R6. Suitable "lower alkenylene" for A1,B and B' means straight or branched one and may include lower alkenylene (e.g. vinylene, 1-propenylene, 2-propenylene, 1-methyl-2 propenylene, 1 or 2 or 3-butenylene, 1 or 2 or 3 or 4pentenylene, 1 or 2 or 3 or t or 5-hexenylene, etc.) and lower alkenylidene (e.g. vinylidene, propenylidene, butenylidene, pentenylidene, hexenylidene, etc.). [n this connection, with regard to the object compounds (I), (Ia), (Ib), (Ic) and (Id) and starting compounds (III) and (IIIe), it is to be noted that when the group represented b Alis lower alkenylene, there is a possibility that these compounds sexist as geometrical isomers, i.e. cis- and trans-isomers and a mixture thereof. For example, with regard to the object com pound (I) wherein the group represented by -A1R3a is 3-carboxy- 1-propenyl, the cis isomer means one geometri cal isomer having the partial structure represented by the following formula: : EMI20.1 and the trans isomer means the other geometrical isomer having the partial structure represented by the following formula: EMI20.2 The cis-and trans isomers as well as a mixture thereof are included within the scope of the present invention. Suitable "organic group which may have suitable substituent(s) "for R3b may include carboxy, protected carboxy group as stated above, an aliphatic and aromatic group, for example, lower alkyl (e.g. methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, neopentyl, tert pentyl, hexyl, etc.); lower alkenyl (e.g. vinyl, 1-propenyl, allyl, 1 methylallyl, 1 or 2 or 3-butenyl, 1 or 2 or 3 or 4-pentenyl, 1 or 2 or 3 or 4 or 5-hexenyl, etc.); aryl (e.g. phenyl, tolyl, xylyl, cumenyl, naphthyl, etc.); ar(lower)alkyl such as phenyl (lower)alkyl (e.g. benzyl, phenethyl, phenylpropyl, etc.), hydroxy(lower)alkyl (e.g., hydroxymethyl, hydroxyethyl, hydroxypropyl , hydroxybutyl, etc.); and the like. Suitable "lower alkoxy" for Q and Q' may include methoxy, ethoxy, propoxy, isopropoxy, butoxy and the like. Suitable example of Y may include an acid residue (e.g. azido, halogen as mentioned above, acycloxy as mentioned above, etc.) and the like. Suitable "amino having a protective group" for H8 and R8 may include phthalimido, succinimido, ethoxycarbonyl- amino and the like, and preferably phthalimido. Suuitable "lower alkyl" for R9 means straight or branched one may include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, hexyl and the like. Suitable "mercapto-protective group" for R10 and R10a may include a conventional protective group such as lower alkyl as mentioned above, ar(lower)alkyl, for example, phenyl(lower)alykl (e.g., benzyl, phenethyl, phenylpropyl, etc.) and the like. Suitable "acid residue" for R11 and z12 may include halogen (e.g. chlorine, bromine, iodine etc.), azide, and the like. The preferable examples of the object compound (I) are exemplified as follows: Preferable example of R1 is amino or acylamino, for example, 2.carboxy-2-(hyd5roxyphenyl)acetamido or a group of the formula: EMI22.1 wherein R5 is amino, lower alkanoylamino or halogen substituted lower alkanoylamino; R6 is lower alkyl, lower alkenyl, lower alkynyl, cycloalkyl, cyclo(lower)alkenyl, aryl[more preferably, phenyl], ar(lower)alkyl[more preferably, phenyl(lower)alkyl] or ar (lower) - alkyl having halogen [more preferably, phenyl(lower)alkyl having halogen] 9 Rê is carboxy; R is a group of the formula : -A1-R3a wherein A1 is hydroxy(lower)alkylene, amino(lower)alkylene, acylamino(lower)alkylene[more preferably lower alkoxycarbonylamino(lower)alkylene], hydroxyirino(lower)zlkylene, lower alkoxyimino (lower)alkylene, lower alkenyloxyimino(lower) alkylene, lower alkenylene or lower alkenylidene, R3a is carboxy ; or z group of the formula EMI23.1 wherein A2 is lower alkylene or lower alkylene having oxo, R3b is carboxy, esterified carboxy [more preferably lower alkoxycarbonyl], lower alkyl, lower alkenyl, hydroxy(lower)alkyl, ar(lower)alkyl [more pre ferably phenyl(lower)alkyl], aryl [more prefera bly phenyl] Q is hydrogen cr lower alkoxy. The processes for preparing the object compounds of the present invention are explained in detail in the following. Process 1: The object compound (I) or a salt thereof can be prepared by reacting the compound (II) or a salt thereof with the comound(Ill) or its reactive derivative at the mercapto group or a salt thereof. Suitable salts of the compound (II) and(III) are each referred to the ones exemplified for the compound (I). Suitable reactive derivative at the mercapto group in the compound (III) may include a metal salt such as an alkali metal salt (e.g. sodium salt, potassium salt, etc.), an alkaline earth metal salt (e.g., magnesium salt, etc.) or the like. The reaction is usually carried out in a solvent such as water, acetone, chloroform, nitrobenzene, methylene chloride, ethylene chloride, dimethylformamide, methanol, ethanol, ether, tetrahydrofuran or any other conventional solvents which. do not adversely influence the reaction, preferably in ones having strong polarity, which may be used as a mixture with water. When the compound ( and/or the compound (III) are used in free form in the reactions, the reaction is preferably carried out in the presence of a base such as alkali metal hydroxide, alkali metal carbonate, alkali metal bicarbonate, trialkylamine, pyridine, or P a Lewis acid sucb as boron trifluoride or the like, and preferably carried out around neutral conditions. The reaction temperature is not critical and the reaction is usually carried out at ambient temperature or under warming. The present invention includes, within its scope, the cases that a protected amino and/or a protected carboxy group are converted into the corresponding free amino and/or the free carboxy group during the reaction or the post-treating step of the present process. Process 2 The object compound (Ia) or a salt thereof can be prepared by reacting the compound (Ib) or its reactive derivatives at the amino group or a salt thereof with an acylating agent. Suitable reactive derivatives at the amino group of the compound tIb) may include conventional ones such as Schiff's base type imino or its tautomeric enamine type derivatives formed by the reaction of the compound (Ib) with a carbonyl compound (e.g. aldehyde, ketone, etc.), isocy2nate; silyl derivatives formed by the reaction of the compound (Ib) with a silyl compound [e.g. bis(trimethylsilyl)acetamide, trimethylsilyl acetamide, etc.); derivatives formed by the reaction of the com pound (Ib) with phosphorus trichloride or phosgene, or the like. Suitable salts of the compound (Ib) can be re ferred to the ones as exemplified for the compound The acylating agent to be used for the present reaction may include one of the formulae: R7 - OH (VI) wherein R7 is acyl, or its reactive derivatives or a salt thereof. Suitable acyls can be rferred to those exem plified hereinbefore. Suitable reactive derivatives of the compound (VI) may include an acid halide, an acid anhydride, an activated amide, an activated ester, and the like. The suitable example may be an acid chloride; an acid azide; a mixed acid anhydride with an acid such as substituted phosphoric acid (e.g. dialkylphosphoric acid, phenylphosphoric acid, diphenylphosphoric acid, dibenzylphosphoric acid, halogenated phosphoric acid, etc.), dialkylphosphorous acid, sulfurous acid, thiosulfuric acid, sulfuric acid, alkylcarbonic acid, ali-thatic carboxylic acid (e.g. pivalic acid, pentanoic acid, isopentanoic acid, 2-ethylbutyric acid or trichloroacetic acid, etc.) or aromatic carboxylic acid (e.g. benzoic acid, etc.); a synnmetrical acid anhydride; ; an activated amide with imidazole, 4-substituted imidazole, dimethylpyrazole, triazole or tetrazole; an activated ester [e.g. cyanomethyl ester, methoxymethyl ester, dimethyliminomethyl [(Q3)2N = CH-] ester, vinyl ester, propargyl ester, pnitrophenyl ester, Z,4-dinitrophenyl ester, trichlorophenyl ester, pentachlorophenyl ester, mesyl-phenyl ester, phenylazophenyl -ester, phenyl thioester, p-nitrophenyl thioester, p-cresyl thioester, carboxymethyl thioester, pyranyl ester, pyridyl ester, piperidyl ester, 8-quinolyl thioester, or an ester with N,N-dimethylhydroxylamine, I hydroxy-2- (lH)pyridone, N-hydroxysuccinimide, N-hydroxyphthalimide or 1-hydroxy-6-chloro-1Hbenzotriazole, and the like. These reactive derivatives can optionally be selected from them according to the kind of the ccm- pound (VI) to be used. The salts of the compound (VI) may be salt with an inorganic base such as an alkali metal salts (e.g. sodium or potassium salt) or an alkaline earth metal salt (e.g. calcium or magnesium salt), a salt with an organic base such as trimethylamine, triethylamine, dicyclohexylamine or the like. The present reaction is usually carried out in a conventional solvent such as water, acetone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvents which do not adversely influence the reaction. Among these solvents, hydrophilic solvents may be used in a mixture with water. When the acylating agent is used in a free acid form or its salt form in the reaction, the reaction is preferably carried out in the presence of a conventional condensing agent such as carbodi Snide compound (e.g. N,N'-dicyclohexylcarbodiimide, N-cyclohexyl-N1 -morpholinoethylcarbodiimide, Ncyclohexyl-N'-(4-diethylaminocyclohexyl)carbodiimide, N,N'diethylcarbodiimide, N,N'-diisopropylcarbodiimide, N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide, etc.), N,N'-carbonylbis(2-mesylimidazole), pentamethyleneketene-N-cyclohexylimine, diphenylkete'ne-N-cyclo- hexylimine, alkoxyacetylene, l-alkoxy-l-chloroethylene, trsalkyl phosphite, ethyl polyphosphate, isopropyl polyphosphate, phosphorus oxychloride, phosphorus trichloride, thionyl chloride, oxalyl chloride, triphenylphosphine, N-ethylbenzisoxavolium salt, N-ethyl-5-phenyl-isoxazolium-3'-sulfonate, 1-(p-chlorobenzenesulfonyloxy)-6-chloro-1H ben=otriazcle, Vilsmeier reagent [e.g. (chloro methylene)dimethylammonium chloride, a compound formed by the reaction of dimethylformamide with phosphorus oxychloride, etc.] or the like. The reaction may also be carried out in the pre sence of an inorganic or an organic base such as an alkali metal bicarbonate, alkali metal carbonate, triClower) alkyl amnne, pyridine, di(lower)alkyl pyridine, N- (lower)alkylmorphorine, N,N-di(lower)- alkylbenzylamine, N,N-di(lower)alkylaniline, or the like. then the base or the condensing agent is in liquid, it can be used also as a solvent. The re action temperature is not critical, and the reaction is usually carried out under cooling or at ambient temperature. Process 3 The object compound (Ic) or a salt thereof can be prepared by subjecting the compound (Id) or a salt thereof to the elimination reaction of the amino protective group. Suitable salts of the compound (Id) may include a metal salt, ammonium salt, an organic amine salt and the like as aforementioned. The present elimination reaction is carried out in accordance with a conventional method such as hydrolysis; reduction; elimination -using a Lewis acid; a method by reacting the compound (Id), wherein the protective group is an acyl group, with an iminohalogenating agent and then with an iminoetherifying agent, and, if necessary, subjecting the resulting compound to hydrolysis; or the like. The hydrolysis may include a method using an acid or base or hydrazine and the like. These methods may be selected depending on the kind of the protective groups to be eliminated. Among these methods, hydrolysis:using an acid is one of the common and preferable methods for elinination of an acyl group. Suitable acid may include an organic or an inorganic acid, for example, formic acid, trifluoro- acetic acid, benzenesulfonic acid, p-toluenesulfonic acid, hyrochloric acid and the like, and preferable acids are those which can easily be removed from the reaction mixture by a conventional marker such as distillation under reduced pressure, for example, formic acid, trifluoroacetic acid, hydrochloric acid, etc. The acid suitable for the reaction can be selected according to the kind of protective groups to be eliminated. When the elimination reaction is conducked with zn acid, it can be carried out in the presence or absence of a solvent. Suitable solvents may include an organic solvent, water or a mixed solvent thereof. lten trifluoroacetic acid is used, the elimination reaction may preferably be carried out in the presence of anisole. The hydrolysis using hydrazine is commonly applied for eliminating the protective groups, for example, succinyl or phthaloyl. The hydrolysis using a base is preferably applied for elimination of an acyl group. Suitable base may include, for example, zn inorganic base such as alkali metal hydroxide (e.g. sodium hydroxide, potassium hydroxide, etc.), alkaline earth metal hydroxide (e.g. magnesium hydroxide, calcium hydroxide, etc.,, alkali metal carbonate (e.g. sodium carbonate, potassium carbonate, etc.), alkaline earth metal carbonate (e.g. magnesium carbonate, calcium carbonate, etc.), alkali metal bicarbonate (e.g. sodium bicarbonate, potassium bicarbonate, etc.), alkali metal acetate (e.g. sodium acetate, potassium acetate, etc.), alkaline earth metal phosphate (e.g. magnesium phosphate, calcium phosphate, etc.), alkali metal hydrogen phosphate (e.g. disodium hydrogen phosphate, dipota ssium hydrogen phosphate, etc.), or the like, and an organic base such zs trialkylamine (e.g. trimethyl- amine, triethylamine, etc.), picoline, N-methyl pyr olidine, N-methylmorpholine, 1,5-diazabicyclo [4,3,0]non-5-ene, 1,4-diazabicyclo[2,2,2]octane, 1,5-diazabicyclo[5,4,0]undecene-5 or the like. The hydrolysis using a base is often carried out in water or a hydrophilic organic solvent or a mixed solvent thereof. among the protective group, the acyl group can generally be eliminated by hydrolysis as mentioned above or by the other conventional hydrolysis. In case that the acyl group is halo(lower)alkoxycarbonyl or 8-quinolyloxyzarbonyl, they are eliminated by treating with a heavy metal such as copper, zinc or the like. The reductive elimination is generally applied for eliminating the protective group, for example, halo(lower or higher)alkoxycarbonyl (e.g. trichloroethoxycarbonyl, etc.), substituted or unsubstituted ar(lowergalkoxycarbonyl (e.g. benzyloxycarbonyl, etc.), etc. Suitable reduction may include, for example, reduction with an alkali metal borohydride (e.g. sodium borohydride, etc.), reduction using a combination of a metal (e.g. zinc, zinc amalgam, etc.) and an organic or inorganic acid (e.g. acetic acid, propionic acid, hydrochloric acid, etc.), catalytic reduction, and the like. Suitable iminohalogenating agents used in method as mentioned about may include phosphorus halide (e.g. phosphorus trichloride, phosphorus pentachloride, phosphorus tribromide, phosphorus pentabromide, etc.), phosphorus oxychloride, thionyl chloride, phosgene and the like. The reaction temperature is not critical, and the reaction is usually carried out at ambient temperature or under cooling. Suitable ininoetherifying agents reacted with thus obtained reaction product may include an alcohol, metal alkoxide and the like. Suitable alcohol may include alkanol (e.g. methanol, ethanol, propanol, isopropanol, butanol, tert-butanol, etc.) which may be substituted with alkoxy (e.g. methoxy, ethoxy, propoxy, isopropoxy, butoxy, etc.) Suitable metal alkoxide may include alkali metal alkoxide (e.g. sodium alkoxide, potassium alkoxide, etc), alkaline earth metal alkoxide (e.g. calcium alkoxy, barium alkoxide, etc.) and the like. The reaction temperature is not critical, and the reaction is usually carried out under cooling at ambient temperature or under warming. Process 4 The object compound (Ie) or P salt thereof can be prepared by subjecting the compound (T;) or a salt thereof to the elimination reaction of the amino protective group. Suitable salts of the compound (If) can be referred to the ones as exemplified for the compound (I). The present elimination reaction is carried out substantially in the same manner as ilustrated in Process 3. The processes for preparing the starting compounds (III) are explained in detail as follows. Suitable reactive derivative at the mercapto group of the compounds (V), (IV), (IIa), (IIIb), (IIIc), (IIId), (IIIe) and (IIIf) can be referred to the ones as exemplified for the compound (III). Suitable salts of the compounds (IV), (IIIa), (IIIb), (IIIc), (IIIe) and (XXV) are such referred to the ones as exemplified for the compound (VI). Suitable salts of the compounds (IIa), (IIb), (IIc), (IIId), (IIIg), (IIIi), (VIa), (IX), (XV) and (XXVI) are each referred to the ones as exemplified for the compound (I). Suitable salts of the compounds (IIIh), (VIII), (X), (XIII), (XVI), (XVII), (XIX), (XX), (XXII) and (XXXIV) may include an inorganic acid salt (e.g, hydroxychloride, hydroxybromide, sulfate, etc.), an organic acid salt (e.g., acetate, p-toluenesulfonate, etc.) and the like. Process A. C (IV) The compound (IV) or Fts reactive derivative at the mercapto group or a salt thereof can be prepared by reacting the compound (V) or its reactive derivative at the mercapto group with the compound (VII) or its ester. Suitable esters of the compound (VII) are referred to the ones exemplified for the ester moiety of the esterified carboxy groups represented by Rê and R . The present reaction may be carried out in the presence of a base such as alkyl alkali metal (e.g. n-butyllithium, etc.), alkali metal acetate (e.g. sodium acetate, etc.), alkali metal alkoxide (e.g. sodium methcxide, potassium tert-butoxide, etc.), alkali metal hydride (e.g. sodium hydride, etc.), alkali metal hydroxide, alkaline earth metal hydroxide or the like. The present reaction is usually carried out in a solvent such as tetrahydrofuran or any other solvents which do not adversely affect the reaction. The reaction temperature is not critical and the reaction is preferably carried out under cooling or at ambient temperature. Process B i) : optional process The compound (IV) or its reactive derivative at the mercapto group or a salt thereof wherein R3 is a protected carboxy group may, if desired, be converted into its free form by elimination of the carboxy protective group, prior to ; ii) introduction of the substituent =N-oR4 ; or iii) conversion of the -CO- group to the -CH(OH) group. In the present elimination reaction, conventional methods used in the elimination reaction of the carboxy protective group, for example, hydrolysis, reduction, elimination using Lexis acid, etc. are applicable. When the carboxy protective group is an ester, it can be eliminated by hydrolysis or elimination using Lewis acid. The hydrolysis is preferably carried out in the presence of a base (e.g. sodium hydroxide, etc.) or an acid (e.g. hydrochloric acid, formic acid, etc.). The present reaction is usually carried out in a solvent such as alcohol (e.g. methanol, etc.) or any other solents which do not adversely affect the reaction. The reaction temperature is not critical and the reaction is preferably carried out under cooling, at ambient temperature or under warming. ii) (IEr) + (ErIII) EMI34.1 (IIIa) The compound (IIIa) or its reactive derivative zt the mercapto group or a salt thereof can be prepared by reacting the compound (IV) or its reactive derivative at the mercapto group or a salt thereof with the compound (VIII) or a salt thereof. The reaction is usually carried out in a conventional solvent such as water, alcohol (e.g., methanol, ethanol, etc.), a mixture thereof or any other ones which do not adversely influence the reaction. When the compound (VIII) is used in its salt form, the reaction is preferably carried out in the presence of an organic or an inorganic base as exemplified before. The reaction temperature is not critical, and the reaction is usually carried out from cooling to heating. iii) (IV) EMI34.2 (IIIb) i) The compound (IIIb) or its reactive derivative at the mercapto group or a salt thereof can be prepared by reducing the compound (IV) or its reactive derivative st the mercapto group or 2 salt thereof. The present reduction can be carried out b a conventional method which is applied to the reduction of the -CO- group to the corresponding -CH(OH)- group, for example, by using a combination of a metal (e.g. zinc, etc.) and an acid (e.g. formic acid, acetic acid, hydrochloric acid, etc;) or a base (e.g. sodium hydroxide, etc.), alkali metal borohydride (e.g. lithium borohydride, etc.), metal amalgam (e.g. aluminum amalgam, etc.) or the like. The present reaction is preferably carried out in a solvent such as alcohol (e.g. ethanol, etc.) or any other solvents which do not adversely affect the react ion. The reaction temperature is not critical and the reaction is preferably carried out under cooling, at ambient temperature or under warming. Process C i) (IIIa) EMI35.1 (hid) The compound (IIId) or its reactive derivative at the mercapto group or a salt thereof can be prepared by reducing the compound (IIIa) or its reactive derivative at the mercapto group or a salt thereof. The present reduction can be carried out by the methods explained in Process B iii), among which the method of using a combination of a metal (e.g. zinc, etc.) and an acid (e.g. formic acid, hydrochloric acid, etc.) are preferred. ii) (IIIddl EMI36.1 (ilic) a) The compound (IIIc) or its reactive derivative at the mercapto group or a salt thereof can be prepared by subjecting the compound (IIId) or its reactive derivative at the mercapto group or a salt thereof to the introduction reaction of the amino group. The present introduction reaction can be carried out by reacting the compound (IIId) or its reactive derivative at the mercapto group or a salt thereof with an acylating agent. Suitable acylating agent may include a) R -OH (XXVII ()wherein R is acyl as defined above) or its reactive derivatives or a salt thereof as explained in Process 2. b) an introducing agent of lower alkoxycarbonyl group, for example, 2-(lower)alkoxycarbonyloxyimino- 2-cyanoacetamide (e.g., 2-ethoxycarbonyloxyimino-2cyanoacetamide, etc.), di (lower) alkyl 2- (lower)- alkoxvcarbony-loxDiminomalonate (e.g. diethyl 2-tertbutoxycarbonyloxyiminomalonate, etc.), lower alkyl 2-(lower)alkoxycarbonyloxyimino-2-cyanoacetate (e.g. ethyl 2-isobutoxycarbonyloxyimino-2-cyanoacetate, etc.), lower alkyl 2- (lower)alkoxycarbonyloxyimino- acetoacetate (e.g. ethyl 2-tert-butoxycarbonyloxy iminoacetoacetate, etc.), lower alkoxycarbonyloxyimino2-phenylacetonitrile (e.g. tert-butoxycarbonyloxyimino2-phenylacetonitrile, etc.) or the like, and the like. The substantially same reaction conditions as set out in Process 2 can be applied in this reaction. Process D (IIIf) EMI36.2 (IIIe) The compound (IIIe) or its reactive derivative at the mercapto group or a salt thereof can be prepared by subjecting the compound (Itif) or its reactive derivative at the mercapto group to the reaction to introduce a carboxy group; whereafter, if necessary, the introduction of a carboxy protective group is carried out. The present reaction can be carried out by a conventional method which is applied for the introduction of a carboxy group to the alkenyl group, for example by reacting the compound (IIIf) with dry ice or carbon dioxide, or the like. The present reaction is usually carried out in the presence of a base as exemplified in Process A. The present reaction is usually carried out in a solvent such as tetrahydrofuran, hexan or any other solvents which do not adversely affect the reaction. The reaction temperature is not critical and the reaction is preferably carried out under cooling or at ambient temperature. Process E i) (XI) EMI37.1 ff The compound (X) or a salt thereof can be prepared by subjecting a compound (XI) to elimination reaction of the amino protective group. This elimination reaction of the amino protective group of the compound (XI) can be carried out in a similar manner to that of aforementioned Process 3. Suitable solvents include water, ethanol, chloroform, diethyl ether and the like. The reaction temperature is not critical and the reaction is usually carried out under warring or heating. ii) (X) @ (IX) EMI38.1 (Via) The compound (Via) or a salt thereof can be prepared by reacting a compound (X) or a salt thereof with a compound (IX) or a salt thereof. The reaction is usually carried out in a conventional solvent such as water, alcohol (e.g. methanol, ethanol, etc.), a mixture thereof or any other ones which do not adversely influence the reaction. When the compound (X) is used in its salt form, the reaction is preferably carried out in the presence of an organic or an inorganic base zs exempli fiend before. The reaction temperature is not critical, and the reaction is usually carried out from cooling to heating. In the present reaction, a syn isomer of the compound (VIa) can be obtained preferably by con ducting the present reaction under around neutral conditions. Process F 1) (XIX) (XVIII) EMI39.1 (XVII) The compound (XVII) or a salt thereof can be prepared by reacting the compound (XIX) or a salt thereof with the compound (XVIII). The present reaction may be carried out in the presence of an organic or inOrganic base suc as alkali metal bicarbonate, alkali metal carbonate, alkaline earth metal bicarbonate, alkaline earth metal carbonate, alkali metal hydroxide, alkaline earth metal hydroxide, tri(lower) alkylamine, N, N-di(lower)alkyl arylamine, cvricine or the like. The preset reaction is usually carried out in a solvent such as acetone, N, N-dimethylformamide or any other solvents which do not adversely affect the reaction. The reaction temperature is nct critical and the reaction is preferably carriec out from at ambient temperature to under heating. 2) (XVII) EMI39.2 (XVI) The compound (XVI) or a salt thereof can be prepared by subjecting the compound(XVII) or a salt thereof to the elimination of the amino-protective group. This elimination reaction can be carried out in a similar manner to that of aforementioned Process 3. 3) (XVI) EMI39.3 (xv) The compound (XV) or a salt thereof can be prepared by reacting the compound(XVI) cr a salt thereof with carbon disulfide. The present reaction is usually carried out in a solvent such as alcohol (e.g., methanol, etc.) or any other solvents which do not adversely affect the reaction. The reaction temperature is not critical and the reaction is preferably carried out under cooling to warming. 4) (XV) r (XIV) EMI40.1 (XIII) The compound(XIII)or a salt thereof can be prepared by reacting the compound(XV) or a salt thereof with a lower alkylating agent (XIV). Suitable "lower alkylating agent mav nclude lower alkyl halide (e.g. methyl iodide, methyl chloride, methyl bromide, ethyl iodide, etc.) and the like. The present reaction is preferably carried out in a solvent such as alcohol (e.g. ethanol, etc.) or any other solvents which do not adversely affect the reaction. The reaction temperature is not critical andthe reaction is preferably carried out under cooling to warming. 5) (XIII)+ (XII) EMI40.2 .( IlIg) The compound (IIIg) or a salt thereof can be prepared by reacting the compound (XIII)or a salt thereof with a hydrazoic acid salt (XII). Suitable "hydrazoic acid salt" may include an alkali metal azido (e.g. sodium azido, potassium azido, etc.) and the like. The present reaction is usually carried out in a solvent such as water, alcohol (e.g., methanol, ethanol, etc.) or a mixed solvent thereof or any other solvents which do not adversely affect the reaction. The reaction temperature is not critical and the reaction is preferably carried out from at ambient to under heating. Process G 1) XX?V) - +(XXIII) EMI41.1 (XXII) The compound (XXII) or a salt thereof can be prepared by reacting the compound (XXIV) or a salt thereof with the compound (XXIII) The present reaction is usually carried out in a solvent such as water, alcohol (e.g., methenol, ethanol, etc.) or a mixed solvent thereof, or any other solvents which do not adversely affect the reaction. The reaction temperature is not cirtical and the reaction is preferably carried out under cooling to warming. 2) (XXII) EMI41.2 (XXI) The compound (XXI) can be prepared by subjecting the compound (XXII) or a salt thereof to the diazotization reaction. The present diazotization can be carried out by using a conventional diazotizing agent such as nitrous acid, [which may be. prepareid.in:situ-by reaction of an alkali metal nitrite with an acid (e.g. hydrochloric acid, etc.)), nitrosyl-chloride, lower alkyl nitrite (e.g., t-butyl nitrite, etc.) or the like. The present reaction may be carried out in the presence of an organic or inorganic acid such as hydrochloric acid, acetic acid or the like. The present reaction may be carried out in a solvent such as alcohol (e.g., methanol, ethanol, etc.) or any other solvents which do not adversely affect the reaction. The reaction temperature is not cirtical and the reaction is preferably carried out under cooling or at ambient temperatura. (3) (XXI) + (XIX) EMI42.1 (xx) The compound (XX) or a salt thereof can be prepared by reacting the compound(XXI) or its reactive derivative at the hydroxy group with the compound (XIX)or a salt thereof. Suitable reactive derivative at the hydroxy group of the compound (XXI) may include the compound (XXI) wherein the hydroxy group is trans formed into an acid residue such as halogen (e.g., chlorine, bromine, etc.), arenesulfonyloxy (e.g., ptoluenesulfonyloxy, p-nitrobenzenesulfonyl , etc.), haloformyloxy (e.g., chloroformyloxy, etc.) or the like. The present reaction can be carried out in a similar manner to that of aforementioned Process F-1). 4) (XX) # (IIIg) The compound (IlIg) or a salt thereof can be prepared by subjecting the coinpound(XX) or a salt thereof to the elimination reaction of the mercaptoprotective group. The present elimination reaction may be carried out in accordance with a conventional method such as hydrolysis using an organic or incrganic acid (e.g. acetic acid, hydrobromic acid, etc.) or the like. The present reaction is usually carried out in a solvent such as water or any other solvents which ao not adversely affect the reaction. The reaction temperature is not cirtical and the reaction is preferably carried out under cooling to heating. Process H: (IIIh) EMI43.1 (IIIg) The compound (IIIg) or a salt thereof can be prepared by isomerizing the compound (IIIh) or a salt thereof. The present isomerization may be carried out in the presence of an organic or inorganic acid (e.g., hydrochloric acid, acetic acid, etc.). The present reaction is usually carried out in a solvent such as water or any other solvents which do not adversely affect the reaction. The reaction temperature is not cirtical and the reaction is preferably carried out under cooling to warming. Process I: (XXV) + (XIX) EMI44.1 (1111) The compound (1111) or a salt thereof can be prepared by reacting the compound (XXV) or its reactive derivative at the carboxy group or a salt thereof with the compound (XIX) or its reactive derivative at the amino group or a salt thereof. The present reaction can be carried out in a similar manner to that of aforementioned Process 2. Process J: (XXVI) EMI44.2 (IIa) The compound (IIa) or a salt thereof can be prepared by reacting the compound (XXVI) or its reactive derinative at the amino group or a salt thereof with an acylating agent. The present reaction can be carried out in a similar manner to that of aforementioned Process 2. Process K : (IIc) EMI44.3 (IIb) The compound (IIb) or a salt thereof can be prepared by subjecting the compound (IIc) or a salt thereof to the elimination reaction of the carboxy-protective group. The present elimination reaction is carried out in accordance with a connectional method such as hydrolysis, elimination using Lewis acid [preferably in the presence of cation trapping agents (e.g., anisole, etc.), reduction or the like. The present reaction is usually carried out in a solvent such as nitroalkane (e.g., nitromethane, etc.), alkylene halide (e.g., methylene chloride, etc.) or any other solvents which do not adversely affect the reaction. The reaction temperature is not cirticul and the reaction is preferably carried out under cooling to warming. The present invention includes, within its scope, the cases that protected amino and/or protected carboxy and/or protected hydroxy group(s) are transformed into the corresponding free amino and/or carboxy and/or hydroxy group(s) according to the reaction conditions and kinds of the protective groups in the course of the aforementioned reactions and/or in post-treatment of the reactions in Processes 1 to4 and A to E. In the aforementioned reactions and/or the posttreating of the reactions in Processes 1 to 4 and A to K of the present invention, the aforementioned geometrical isomer and/or tautomeric isomer may occasionally be transformed into the other geometrical isomer and/or tautomeric isomer and such cases are to be also included in the scope of the present invention. In case that the object compound (I) has a free carboxy group and/or a free amino group, it may be transformed into its pharmaceutically acceptable salt as aforementioned by a conventional method. The object compound (I) of the present invention exhibits high antimicrobial activity and inhibits the growth of a number of microorganisms including pathogenic Gram-positive and Gram-negative bacteria. For therapeutic administration, the cephalosporin compounds according to the present invention are used in the form of pharmaceutical preparation which contain said compounds in admixture with a pharmaceutically acceptable carriers such as an organic or inorganic solid or liquid excipient suitable for oral, parenteral or external administration. The pharmaceutical preparations may be in solid form such as capsule, tablet, dragee, ointment or suppository, or in liquid form such as solution, suspension, or emulsion. If desired, there may be included in the above preparations auxiliary substances, stabilizing agents, wetting or emulsifying agents, buffers and other commonly used additives. stile the dosage of the compounds may vary from and also depend upon the age and condition of the patient, an average single dose of about 50 mg., 100 mg., 250 mg., and 500 mg. of the compounds according to the present invention has proved to be effective for treating of infectious diseases caused by a number of pathogenic bacteria. In general amounts, daily dose between 1 mg/body and about 1000 mg/body or even more may be administered. Now, in order to show the utility of the object compounds (I), test data on anti-microbial activity of a representative compound of the present invention are shown below. Test method One loopful of an overnight culture of each test S strain in Trypticase-soy broth (10 viable cells per ml.) was streaked on heart infusion agar (HI-agar) containing graded concentrations of antibiotics, and the minimal inhibitory concentration (MIC) was expressed in terms of vg/mQ after incubation at 370C for 20 hours. Test compound (1) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamido]-3-[1-2-carboxy-2-methoxyiminoethyl)-1Htetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid (syn isomer : in the 7-position). (2) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxylic acid (syn isomer : in the 7position). (3) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2-aminoethyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer : in the 7-position). ¯¯¯ (4) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] -3-[1-[3-(4-methyl-1-piperazinyl)propyl]-1H-tetrazol5-yl]thiomethyl-3-cepghem-4-carboxylic acid (syn isomer). (5) 7-(2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido)-3-[1-(2-(4-methyl-1-piperazinyl)ethyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer). (6) 7-(2-methoxyimino-2-(2-aminothiazol-4-yl) acetEmico]-3-[l-(/-methyl-l-?i?erazinyl)c2rbsnylmethyl- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer). Test result EMI49.1 <SEP> M.I.C. <SEP> ( g/ml) <tb> Test <tb> Microorganism <SEP> Compound <SEP> Compound <SEP> Compound <SEP> Compound <SEP> Compound <SEP> Compound <tb> <SEP> (1) <SEP> (2) <SEP> (3) <SEP> (4) <SEP> (5) <SEP> (6) <tb> Klebsiella <tb> pnuemoniae <SEP> 20 <SEP> 0,20 <SEP> 0,39 <SEP> 0,39 <SEP> 0,10 <SEP> 0,10 <SEP> 0,10 <tb> Proteus <SEP> 0,05 <SEP> 0,39 <SEP> 0,10 <SEP> 0,39 <SEP> 0,39 <SEP> 0,10 <tb> vulgaris <SEP> 2 <tb> The following prepararions and examples are given for the purpose of illustrating the present invention. Preparation 1 A solution of l-methyl-lH-tetrazole-5-thiol (2s g) in dry tetrahydrofuran (80 ml) was added over 20 minutes with stirring under dry nitrogen atmosphere to a n-butyllithium (250 ml, 158 in hexane) solution in dry tetrahydrofuran (250 ml) precooled to -15 to -100C. After stirring at the same temperature for 40 minutes, the mixture was cooled to -600t50C and thereto was added dropwise a solution of diethyl oxalate (28.95 g) in dry tetrahydrofuran (80 ml) over 30 min. The reaction mixture was stirred at -60 #5 C for 1.5 hours. 10% Aqueous hydrochloric acrid (72 ml) was added with stirring and the separated aqueous layer (pH 7.5) was adjusted to pH 2.5 with 10 hydrochloric acid and extracted with ethyl acetate (200 ml x 3). The combined extracts were dried over magnesium sulfate and evaporated to give oily residue of l-ethoxalylmethyllH-tetrazole-5-thiol (34.3 g). I.R. (Nujol) : 1730 cm 1 N.M.R. (CDcl3, 6) : 1.43 (3H, t, J=7Hz), 4.46 (2H, q, J=7Hz), 5.67 (2H, s) Preparation 2 To a solution of l-ethoxalylmethyl-lH-tetrazole-5thiol (18.6 g) in methanol (186 ml) was added 1N aqueous solution of sodium hydroxide (172 ml) at room temperature and the mixture was stirred for 40 minutes. After adjusting to pH 7.5 with 10 hydrochloric acid, the solution was evaporated in vacuo to remove methanol and the remained aqueous solution was washed with ethyl acetate (100 ml). The aqueous solution was adjusted to pH 4.0 and washed again with ethyl acetate to remove impurities. The aqueous solution was adjusted to pH 2 i, and extracted with ethyl acetate, dried over magnesium sulfate and evaporated to give pale yellow powder of 1-oxalomethyl-1H-tetrazole-5-thiol (9.7 g), mp 140 to 1500C (dec.). I.R. (Nujol) : 1750, 1710 cm 1 N.M.R. (DMSO-d, #): 5.71 (2H, s) Preparation 3 A solution of 1-oxalomethyl-1H-tetrazole-5-thiol (10.0 g) and hydroxylamine hydrochloride (4.8 g) in water (100 ml) was adjusted to pH 6.0 with 2N aqueous solution of sodium hydroxide and stirred at room temperature for 3.5 hours. The solution was acidified with 10% hydrochloric acid to pH 2.0, extracted with a mixture of tetrahydrofuran and ethyl acetate (1:1). The extract was washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and evaporated to give l-(2-carboxy-2-hydroxyimino- ethyl)-lH-tetrazoie-5-thiol (5.61 g) N.M.R. (DMSO-d6, #): 5.19 (2H, s) Preparation 4 A solution of l-oxalomethyl-lH-tetrazole-5-thiol (1.09 g) and methoxyamine hydrochloride (0.47 g) in water (15 ml) was adjusted to pH 6.5 with sodium bicarbonate and stirred at room temperature for 2 hours. After washing with ethyl acetate, the aqueous solution was adjusted to pH 1.8 with 10% hydrochloric acid, extracted with ethyl acetate, dried over magnesium sulfate and evaporated to gire l-(2-carboxy-2- methoxyiminoethyl)-1H-tetrazole-5-thiol (1.05 g), mp 84 to 86 C. I.R. (Nujol) 16±5 cm 1 N.M.R. (DMSO-d6, #) : 3.97 (3H, s), 5.17 (2H, s) Preparation 5 The following compound was prepared according to the similar manners to those of Preparation 1 to 4. 1-(2-Carboxy-2-allyloxyiminoethyl)-1H-tetrazole-5-thiol. I.R. (Nujol) : 1700 cm N.M.k. (DMSO-d6, 6) 4.58 (2H, m), 4;98-5.33 (4H, m), 5.58-6.07 (1H, m) Preparation 6 l-Oxalomethyl-lH-tetrazole-5-thiol (0.94 g) was dissolved in aqueous 60% ethanol (15 ml) containing sodium hydroxide (1.4 g) and to this solution was added portionwise zinc powder (0.98 g). The mixture was stirred at room temperature for 3.5 hours. After removal of zinc powder by filtration, ethanol was removed under reduced pressure and the remained aqueous solution was adjusted to pH 2.0 with 10% hydrochloric acid. Extraction with ethyl acetate, drying over magnesium sulfate and evaporation gave an oil which was triturated with a mixture of diisopropyl ether and n-hexane (1:1). The crystals were filtered and washed with a mixture of diisopropyl ether and n-hexane (1:1) to give 1-(2-carboxy-2-hydroxyethyl)-1H-tetrazole-5thiol (0.40 g), mp 143 to 1460C, I.R. (Nujol) : 3320, 3180, 1715 cm 1 N.M.R. (DMSO-d6, 6) : 4.17-4.57 (3H, m) Preparation 7 A mixture of 1-(2-carboyx-2-hydroxyiminoethyl)-1H tetrawole-5-thiol (19.3 g), 5C% formic acid (255 ml), zinc powder (22.3 g) in ethanol (230 ml) was stirred for 5 hours at room temperature. After the addition of trifluoroacetic acid (100 ml), the mixture was stirred for 5 minutes and filtered. The filtrate was evaporated under reduced pressure. The residue was adjusted to pH 13.0 with 4N aqueous solution of sodium hydroxide and then filtered. The filtrate containing disodium l-(2-carboxylate-2-aminoethyl)-lH-tetrazole- 5-thiolate was adjusted to pH 6.0 and thereto were added dioxane (300 ml), 2-tert-butoxycarbonyloxyimino2-phenylacetonitrile (35.1 g) and triethylamine (26.2 g). The resulting mixture was stirred overnight at room temperature. The reaction mixture was adjusted to pH 7.0 and washed with ethyl acetate. The washed layer was adjusted to pH 2.0 with phosphoric acid and then extracted with ethyl acetate. The extract was washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and evaporated. The -residue was pulverized in diisopropyl ether to give l-C2-carboxy-2-tert-butoxyzarboxamidoethyl)-lH- tetrazole-5-thiol (21.2g) I R. (Nujol) : 3400, 1718, 1690 cm N.M.H. (DMSO-d6, 6) 1.34 (9H, s), 4.33-4.80 (3H, m), 7.27 (1H, broad s) Preparation 8 158 solution of n-butyl lithium in hexane (371 ml) was dissolved in dry tetrahydrofuran (150 ml) and cooled to -200C. To the solution was added dropwise a solution of l-allyl-lH-tetrazole-5-thiol (38.7 g) in tetrahydrofuran (220 ml) with stirring under a stream of nitrogen gas during a period of 30 minutes. After the addition of solid carbon dioxide, the mixture was stirred for 30 minutes at -100C. To the mixture was added water (275 ml) and then the resulting mixture was stirred. The aqueous layer was separated adjusted to pH 4.5, washed with ethyl acetate, adjusted to pH 3.8 to 4.0 and extracted with ethyl acetate. The extract was washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and then evaporated. The residue was washed with diisopropyl ether to give an oil of 1-(3-carboxy-1-propenyl)-1H-tetrazole-5- thiol (5.5 g). I.R. (Nujol) : 1700 cm 1 N.M.R. (DMSO-d6, #) : 3.57 (2H, m), 6.00 (lH, m), 7.05 (1H, m) The remaining aqueous layer was adjusted to pH 1.5 and extracted with ethyl acetate. The extract was washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and evaporated to give 1-(1-carboxyallyl)-1H-tetrazole-5-thiol (21.3 g). I.R. (Film) : 1710, 1620 cm N.M.R. (DMSO-d6, 6) : 5.30-5.70 (2H, m), 5.90-6.67 (2H, m) Preparation 9 To a solution of 2-(2-formamidothiazol-4-yl)glyoxylic acid (30 g) and sodium bicarbonate (12.6 g) in water (1300 ml) was added allyloxyamine hydrochloride (19.8 g), and the mixture was stirred for 7 hours at ambient temperature at pH 6. To the reaction mixture was added ethyl acetate (500 ml). After the mixture was adjusted to pH 1.9 with 10% hydrochloric acid, the ethyl acetate layer was separated. The ethyl acetate layer was washed with an aqueous solution of sodium chloride, dried over magnesium sulfate and then the solvent was distilled off. The residue was pulverized in diisopropyl ether, collected by filtration and then dried to give 2-allyloxyimino-2-(2-formamido- thiazol-4-yl)acetic acid (syn isomer)(25.3 g). I.R. (Nujol) : 3110, 1730, 1660, 1540 cm N.M.H. (d6-DMSO, 6) : 4.70 (2H, m), 5.13-5.60 (2H, m), 5 73-6.27 (1H, m), 7.57 (1H, s), 8.35 (1H, s) Preparation 10 (1) Ethyl 2-(2-propynyloxyimino)-3-oxobutyrate (syn isomer)(71.2 g) was obtained by reacting ethyl 2-hydroxyimino-3-oxobutyrate (syn isomer)(56.7 g) - with 2-propynyl bromide (43 g) in the presence of potassium carbonate (72.3 g) and N,N- dimethylformamide (280 ml). I.R. (Film) : 3280, 3220, 2120, 1735, 1670 cm 1 (2) Ethyl 2-(2-propynyloxyimino)-3-oxo-4-chlorobutyrate (syn isomer)(61.6 g) was obtained by reacting ethyl 2-(2-propynyloxyimino)-3-oxobutyrate (syn isomer) (71.2 g) with sulfuryl chloride (50.2 g) in acetic acid (81 ml). I.H. (Film) : 3300, 2130, 1745, 1720, 1675 cm 1 (3) Ethyl 2- (2-propynyloxyimino)-2-(2-aminothiazol-4- yl)acetate (syn isomer) (35.6 g) was obtained by reacting ethyl 2- (2-propynyloxyimino)-3-oxo-4- chlorobutyrate (syn isomer) (61 g) 'with thiourea (20 g) in the presence of sodium acetate trihydrate (35.8 g), water (150 ml) and ethanol (180 ml). I.R. (Nujol) : 3290, 2220, 1729 cm-1 (4) 2-(2-Propynyloxyimino)-2-(2-aminothiazol-4-yl)acetic acid (syn isomer)(1.924 g) was obtained by hydrolyz ing ehtyl 2-(2-propynyloxyimino)-2-(2-aminothiazol 4-yl)acetate (syn isomer)(2.8 g) in the presence of 1N aqueous solution of sodium hydroxide (22.17 ml), methanol (2 ml) and tetrahydrofuran (20 ml). I R. (Nujol) : 2190, 1740 cm (5) 2-(2-Propynyloxyimino)-2-[2-(2, 2, 2-trifluoro acetamido)-thiazol-4-yl]acetic acid (syn isomer) (11.4 g) was obtained by reacting 2-(2-propynyloxyimino)-2-(2-aminothiazol-4-yl) acetic acid (syn isomer)(10.0 g) with 2,2,2 trifluoroacetic anhydride (22.5 g) in the presence of bis(trimethylsilyl)acetamide (22.3 g) and dry ethyl acetate (100 ml). I.R. (Nujol) : 3280, 3130, 2140, 1710, 1575, 1360, 1260, 1210, 1165, 1075, 1020, 980, 750 cm 1 N.M.R. (DMSO-d6, #): 3.53 (1H, t, J=2Hz), 4.83 (2H, d, J=2Hz), 7.73 (1H, s). Preparation 11 (1) The following compounds were obtained according to 2 similar manner to that of Preparation 10(1). i) Ethyl 2-cyclopentyloxyimino-3-oxobutyrate (syn isomer), oil. I.R. (Film) : 170, 1670, 1495, 1430 cm 1 NM.R. (CCl4, #) : 1.32 (3H, t, J=7Hi), 1.4-2.2 (8H, m), 2.33 (3H, s), 4.27 (2H, q, J=7Hz) 4.87 (1H, m) ii) Ethyl 2-benzyloxyimino-3-oxobutyrate (syn isomer). iii) Ethyl 2-(4-fluorobenzyloxyimino)-3-oxobutyrate (syn isomer). I.R. #maxFilm: 3000, 2940, 1730, 1690, 1600 cm-1 N.M.R. #(DMSO-d6, ppm): 1.21 (3H, t, J=7.0Hz), 2.34 (3H, s), 4.26 (2H, q, J=7.OHz) , 5.32 (2H, s), 6.97-7.73 (4H, m) (2) The following compounds were obtained according to a similar manner to that of Preparation 10(2). i) Ethyl 2-cyclopentyloxyimino-3-oxo-4-chlorobutyrate (syn isomer) , oil. I.R. (Fi-lm) : 1750, 1735, 1465, 1435 cm N.M.R. (CCl4, 6) : 1.33 (3H, t, J=7Hz), 1.3-2.4 (8H, m), 4.28 (2H, q, J=7Hz), 4.46 (2H, s), 4.86 (1H, m) ii) Ethyl 2-benzyloxyimino-3-oxo-4-chlorobutyrate (syn isomer). iii) Ethyl 2-(4-fluorobenzyloxyimino)-3-oxo-4 chlorobutyrate (syn isomer). I.R. #maxFilm: 1720, 1600 cm-1 N.M.R. # #(DMSO-d6, ppm) : 1.20 (3H, t, J=7.0Hz), 4.28 (2H, q, J=7.0Hz), 4.87 (2H, s), 5.36 (2H, s), 7.00-7.75 (4H, m) (3) The following compounds were obtained according to a similar manner to that of Preparation 10(3). i) Ethyl 2-cyclopentyloxyimino-2-(2-aminothiazol-4 yl)acetate (syn isomer), mp 134 to 136 C. I.R. (Nujol) : 3490, 3450, 3250, 3120, 1735, 1540, 1460 cm N.M.R. (DMSO-d6, #): 1.25 (3H, t, J=7Hz) 1.62 (8H, broad s), 4.27 (2H, q, J=7Hz), 4.70 (1H, m), 6.85 (1H, s), 7.20 (2H, s) ii) Ethyl 2-benzyloxyimino-2-(2-aminothiazol-4-yl) acetate (syn isomer). I.R. #maxNujol: 3440, 3240, 3100, 1730, 1680 cm 1 iii) Ethyl 2-(4-fluorobenzyloxyimino)-2-(2 aminothiazol-4-yl)acetate (syn isomer). I.R. #maxNujol: 3450, 3150, 3100, 1710, 1620 cm-1 N.M.R. #(DMSO-d6, ppm) 1 1.23 (3H, t, J=7.0Hz), 4.30 (2H, q, J=7'0Hz), 5.15 (2H, s), 6.90 (1H, s), 6.95-7.60 (4H, m) 4) The following compounds were obtained according to a similar manner to that of Preparation 10(4). i) 2-Cyclopentyloxyimino-2-(2-aminothiazol-4-yl) acetic acid (syn isomer), mp 186 C (dec.). I.R. (Nujol) : 3330, 3120, 1635, 1450 cm 1 N.M.R. (DMSO-d6, 6) : 1.1-2.2 (8H, m), 4.68 (1H, m), 6.81 (1H, s), 7.18 (2H, broad s) ii) 2-Benzyloxyimino-2-(2-aminothiazol-4-yl)acetic acid (syn isomer). I.R. #maxNujol:3330, 3200, 3100, 1660, 1590 cm-1 N.M.R. #(DMSO-d6, ppm) : 5.20 (2H, s), 6.90 (1H, s), 7.40 (5H, s) iii) 2-(4-Fluorobenzyloxyimino)-2-(2-aminothiazol-4 yl)acetic acid (syn isomer). I.R. #maxNujol: 3650, 3450, 3300, 3150, 1630 cm-1 N.M.R. #(DMSO-d6, ppm) : 5.16 (2H, s), 6.88 (1H, s), 7.04-7.66 (4H, m) (5) The following compounds were obtained according to a similar manner to that of Preparation 10(5). i) 2-Cyclopentyloxyimino-2-[2-(2, 2, 2 trifluoroacetamido)thiazol-4-yl]acetic acid (syn isomer). I.R. (Nujol) : 3200, 3130, 1720, 1590, 1580 cm N.M.R. #(DMSO-d6, 6) : 1.34-2.22 (8H, m), 4.81 (1H, m), 7.71 (1H, s) ii) 2-Benzyloxyimino-2-[2-(2, 2, 2-trifluoroacetamiso). thiazol-4-yl)acetic acid (syn isomer). I.R. (Nujol) : 3130, 1730, 1600, 1580 cm 1 N.M.R. (DMSO-d6, 6) : 5.23 (2H, s), 7.36 (5H, s), 7.67 (lH, s) ii) 2-(4-Fluorobenzyloxyimino)-2-[2-(2, 2, 2 trifluoroacetamido)thiazol-4-yl]acetic acid (syn isomer), mp 180-1820C. I.H. V Nujol : 3200, 3150, 1730 cm max N.M.H. 6(DMSO-d6, ppm) : 5.25 (2H, s), 7.02-7.60 (4H, m), 7.72 (1H, s) Presaration 12 A mixture of 3-chlorocyclopentene (36.9 g), N-hydroxyphthalimide (58.2 g) and triethylamine (53.9 g) in acetonitrile (370 ml) was refluxed for 2 hours. The reaction mixture was poured into ice-water. The precipitated crystals were collected by filtration, washed with water and dried over phosphorus pentoxide to give N-(2-cyclopenten-1-yl)oxyphthalimide (56.5 g). I.R. (Nujol) : 1780, 1730, 1610 cm -l N.M.H. (DMSO-d6, 6) : 1.98-2.9 (4H, m), 5.42 (lH, s), 6.00 (1H, m), 6.28 (1H, m), 7.92 (4H, s) PreParation 13 A mixture of N-phenoxyphthalimide (5.5 g) and hydrazine hydrate (1.15 g) in ethanol (120- ml) was stirred for 1.5 hours at 75 to 80 C. After the addition of conc.hydrochloric acid (4 ml) and water (40 ml) to the reaction mixture under ice-cooling, the resulting mixture was filtered and the filtrate was evaporated. The remaining mixture containing phenoxyamine hydrochloride was adjusted to pH 7.0 with 10% aqueous solution of sodium hydroxide and thereto were added 2-(2 formamidothiaLol-4-yl)glyoxylic acid (3.54 g). The resulting mixture was adjusted to pH 5.0 to 5.5 with 10% hydrochloric acid and stirred for 2 hours at room temperature. The reaction mixture was evaporated under reduced pressure and to the residue was added ethyl acetate. The resulting mixture was adjusted to pH 7.0 with a saturated aqueous solution of sodium bicarbonate. The aqueous layer was separated, adjusted to pH 2.0 with 10% hydrochloric acid and extracted with ethyl acetate. The extract was washed with a saturated aqueous solution of sodium chloride. The solution was dried over magnesium sulfate and concentrated under reduced pressure. The residue was pulverized in diethyl ether to give 2-phenoxyimino-2-(2-formamidothiazol-4 yl)acetic acid (syn isomer). -l I.R. (Nujol) : 3200, 1700, 1690, 1590, 1560 cm N.M.R. (DMSO-d6, 6) : 12.60 (1H, broad s), 8.58 (1H, s), 7.85 (1H, s), 7.73-6.67 (5H, m) Preparation 14 The following compound was obtained according to a similar manner to that of Preparation 13. 2- (2-Cyclopenten-l-yl)oxyimino-2-(2-formamidothiazol- 4-yl)acetic acid (syn isomer). I.R. (Nujol) : 3200, 1740, 1710, 1600, -1560 cm 1 N.M.R. (DMSO-d6, 6) : 1.52-2.67 (4H, m), 5.45 (1H, m), 6.00 (1H, m) , 6.28 (1H, m), 7.67 (1H, s), 8.73 (1H, s), 13.0 (1H, broad s) Preparation 15 (1) To the solution of N-(3-bromopropyl) phthalimide (402 g) in acetone (2.5 Q) were addled 1-msthylpiperazine (225 g) and potassium carbonate (415 g). The resulting mixture. was reflxed with stirring for 3.5 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with acetone (300 ml). The filtrate and washing were combined and evaporated under reduced pressure. The remaining starting materials in the residnal oil were azeotropically removed with benzene to give an oil of N-[3-(4-methyl-1-piperazinyl)propyl]phthalimide (532 g). I.R. (Film) : 1770,1700 cm (2) To a solution of N-[3-(4-methyl-1-piperazinyl) propyl]phthalimide (502 g) in ethanol (3.0±) was added 190% hydrazine hydrate (187.6 g). The nesulting mixture was refluxed wit stiving for 1.5 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with ethanol (1 l). The filtrate and washing were combined and evaporated, under reduced pressure. The residual oil was distilled under reduced pressure to give 3-(4-methvl-1- piperazinyl) propylamine $146.6 g), bp 34 mmHg/127 to 128 C. (3) To a solution of potassium hydroxide (57.3 g) in methanol (250 m±) was added 3-(4-methyl-l-piperazinyl) propylamine (146 g) and thereto was added carbon disulfid (70.6 g) with stirring under ice cooling over a period of 40 minutes. The resulting mixture was stirred for 3.5 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water (400 ml) and washed with diethyl ether twice. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (132.1 g) with stirring. The resulting mixture was stirred for 2 hours under ice-cooling, and extracted with ethyl acetate (400 ml x 3) and chloroform (400 mQ x 2). The extracts were combined, dried over magnesium sulfate and then evaporated under reduced pressure to give methyl N-[3-(4-methyl-1-peperazinyl)propyl]dithiocarbamate (139.3 g). Thus obtained product was used directly in she next step reaction without further purification. (4) To a solution of methyl N-[3-(4-methyl-l-piperazinyl) propyl dithiocarbamate obtained in Preparation 1 (3) (139.3 g) in a mixture of water (420 mQ) and ethanol (280 mQ) was added sodium azide. (47.6 g). The resulting mixture was refluxed with stirring for 3 hours. The reaction mixture was concentrated under reduced pressure. The concentrate was washed successively with ethyl acetate and diethyl ether and then evaporated. To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure, and to the residual oil was added 6N hydrochloric acid. The mixture was evaporated under reduced pressure. The residue was recrystallized from isopropyl alcohol containing water to give 1-[3-(4methyl-1-piperazinyl)propyl]-1H-tetrazole-5-thiol dihydrochloride(481 g), mp 239 to 243 C. N.M,R. (D20, 6): 2.3-2.8 (2H, m), 3.07 (3H, s) 3.3-3.7 (2H, m), 3.75 (8H, s), 4.42 (2H, t,J = 6Hz). Preparation 16 (1) To the solution of N-(3-bromopropyl)p'nthalimide (20.1 g) in acetone (225 m±) were added. l-benzyl piperazine (19.8 g) and potassium carbonate (31.1 g). The resulting mixture was refluxed with stirring for 3 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with acetone. The filtrate and washing were combined and evaporated under reduced pressure to give the oily crude product of the object compound (36.2 g). This oily product was subjected to column chromatography on silica gel. The fractions containing the object compound were collected to give an oil of N-[3- (4-benzyl-l-piperazinyl)propylphthalimide (22.4 g). (2) To a solution of N- [ 3- ( 4-benzyl- l-piperaz inyl) propyl] pkthalimide (22.4 g) in ethanol (250 m ) was added 100% hydrazine hydrate (8.02'g). The resulting mixture was refluxed with stirring for 2.5 hours. The-. reaction mixture was cooled and then filtered. The filter cake was washed with ethanol. The filtrate and washing were combined and evaporated under reduced pressure. To the residue was added 5% aqueous solution of potassium hydroxide and the mixture was extracted with chloroform. The extract was washed with water, dried over magnesium sulfate and then evaporated under reduced pressure to give an oil of 3-(4 benzyl-l-piperazinyl)propylamine (12. 4 g). N.M.R. (CDCQ3, S): 1.4 (2H, s), 1.6 (2H, tt, J = 7Hz), 2.4 (2H, t, J = 7Hz), 2.47 (8H, s), 2.73 (2H. t, J= 7Hz) , 3.5 (2H, s) , 7.27 (5H, s) (3) To a solution of potassium hydroxide (1.3 g) in methanol (9 ml) was added 3-(4-benzyl-l-piperazinylY- propylamine (4.9 g) and thereto was added carbon disulfide (1.8 g) under ice cooling over a period of 1.0 minutes. The resulting mixture was stirred for 4 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water (20 mQ) and washed with diethyl ether. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (3.0 g) with stirring under ice-cooling over a period of 10 minutes. The resulting mixture was stirred for 4 hours under ice-cooling. The reaction mixture was extracted with ethyl acetate, dried over magnesium sulfate and then evaporated under reduced pressure to give an oil of methyl N-[3-(4-benzyl-1-piperazinyl) propyl) dithiocarbamate (4.6 g). (4) To a solution of methyl N- [3- (4-benzyl-l-piperazinyl) propyl]dithiocarblmate (4.6 g) in a mixture of water (12 mQ) and ethanol (8 mi) was added sodium azide (1.2 g). The resulting mixture was refluxed with stirring for 3 hours. The reaction mixture was concentrated under reduced pressure. The concentrate was washed successively with ethyl acetate and diethyl ether and.then evaporated. -To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure. T6 the residue was added 6N hydrochloric acid. The mixture was evaporated under reduced pressure and the residue was recrystallized from isopropyl alcohol containg water to give 1- [3- (4-benzyl-l-piperazinyl) propyl) -lH-tetrazole-5- thiol dihydrochloride (2.5 g), mp 234 to 2370C. N.X.R. (DMSO-d6, 6): 2.1-3.0 (2E, m), 3.1-4.1 (10H, m) e; 4.42 (2H, s), 4.4 (2H, t), 7.3-8.0 (5H, m) Preparationl7 (1) To the solution of N-(3-bromopropyl)phthalimide (38.8 g) in acetone (400 m±) were added. 1-ally-piperazine (18.5 g) and potassium carbonate (60.8 g). The resulting mixture was refluxed with stirring for 5 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with acetone. The filtrate and washing were combined and evaporated under reduced pressure. The residual oil was subjected to column chromatography on silica gel. The fractions containg the object compound were collected and then evaporated under reduced pressure to give an oil of N-[3-(4-allyl-1- piperazinyl)propyl]phthalimide (35.6 g). (2) To a solution of N-[3-(4-allyl-1-piperazinyl)propyl] phthalimide (35.6 g) in ethanol (400 m±) was added 100% hydrazine hydrate (14.3 g). The resulting mixture was refluxed with stirring for 2.5 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with ethanol. The filtrate and washing were combined and evaporated .under'reduced -pressure. The residual oil was subjected to column chromatigraphy (aluminum oxide), eluting with a mixture of chloroform and methanol (10:1). The fractions containing the object compound were collected and then evaporated under reduced pressure to give an oil of 3-(4-allyl-l-piperazinyl)propylamine (18.8 g). tl.M.P. (CCQ4,.): 1.43 (2H, s) , 1.52 (2H, tt, J = 7Hz), 2.4 (8H, broad s), 2.33 (2H, t, J = 7Hz), 2.67 (2H, t, J = 7Hz), 2.92 (2H, d, J = 6Hz), 5.0-6.0 (3H, m). (3) To a solution of potassium hydroxide (6.3 g) in methanol (40 m ) was added 3-(4-allyl-l-piperazinyl)propylamine (18.8 g) and thereto was added carbon disulfide (7.8 g) under ice cooling over a period of 10 minutes. The resulting mixture was stirred for 2 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water and washed with diethyl ether. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (14.6 g) with stirring. The resulting mixture was stirred for 2 hours under ice-cooling. The reaction mixture was extracted with ethyl acetate, the extract was washed with a saturated aqueous solution of sodium chloride, dried over magnesium. sulfate and then evaporated under reduced pressure to give an oil of methyl N-[3-(4-allyl-1-piperazinyl)propyl3¯ dithiocarbamate (17.7 g). (4) To a solution of methyl N-[3-(4-allyl-l-piperazinyl) propyl)dithiocarbamate (17.7 g) in a mixture of water (60 mQ) and ethanol (40 mQ) was added sodium azide (5.5 g). The resulting mixture was ref fluxed with stirring for 3 hours. The reaction mixture was concentrated under reduced pressure. The concentrate was washed successively with ethyl acetate and dimethyl ether and then evaporated. To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure. To the residue was added 6N hydrochlpric acid. The mixture was evaporated under reduced pressure. The residue was recrystallized from methanol containing water to give 1-[3- (4-allyl-l-piperazinyl) propyl] -lH-tetrazole-S-thiol dihydrochloride (2.5 g), mp 211 to 2150C (dec.) N.M.R. (D20, 6): 2.2-2.8 (2H, m), 3.78 (8H, s), 3.67 (2H, t, J = 6Hz) 3.97 (2H, d, J = 7Hz), 4.50 (2H, t, J = 7Hz), 5.5-6.5 (3H, m). Preparation 18 (1) To the solution of N-(3-bromopropyl) phthalimide (40.5 g) in acetone (375 mQ) were added l-(2-hydroxyethyl) piperazine (31.5 g) and potassium carbonate (63.0 g). The resulting mixture was refluxed with stirring for 3 hours. The reaction mixture was filtered and the filtrete was evaporated. The residual oil was subjected to column chromatography on silica gel, eluting with a mixture of chloroform and methanol (9;1). The fractions containing the object compound were collected and then evaporated to give an oil of N-[3-[4-(2-hydroxyethyl)-1-piperazinyl] propyl]phthalimide (25.5 g). (2)To a solution of N-[3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl] phthalimide (14.4 g) in ethanol (145 mQ) was added 100% hydrazine hydrate (6.6 g). The resulting mixture was refluxed with stirring for 2 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with ethanol. The filtrate and washing were combined and evaporated under reduced pressure. The residual oil was subjected to column chromatography (Aluminum oxide), eluting with a mixture of chloroform and methanol (20:1). The fractions containing the object compound ere collected and then evaporated to give an oil of 3-[4-(2-hydroxyethyl) l-piperazinyl)] propylamine (5.0 g). N.M.R. (DMSO-d6, S) 1.48 (2H, tt, J = 7Hz), 2.28 (2H, t, J = 7Hz), 2.53 (2H, t, J = 7Hz), 2.1-2.7 (lOH, m), 3.03 (3H, s), 3.15 (2H, t, J = 7Hz). (3) To a solution of potasium hydroxide (1.5 g) in methanol (11 mQ) was added 3-[4-(2-hydroxyethyl)-1-piperazinyl] propylamine (5.0 g) and .hereto was added carbon disulfide (2.0 g) under ice cooling over a period of 10 minutes. The resulting mixture was stirred for 2 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water (10 mQ) washed with diethyl ether. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (3.8 g) with stirring. The resulting mixture was stirred for 2 hours under ice-cooling, extracted with ethyl acetate, dried over magnesium sulfate and then evaporated to give methyl N-[3-[4-(2-hydroxy-ethyl)-1-piperazinyl]propyl]dithio-carbamate (4.8 g). (4) To a solution of methyl N-[3-[4-(2-hydroxy-ethyl)- 1-piperazinyl]-propyl]-dithiocarbamate (4.8 g) in a mixture of water (15 mQ) and ethanol (10 m±) was added sodium azide (1.5 g). The resulting mixture was refluxed with stirring for 4 hours. The reaction mixture was concentrated under reduced pressure. The concentrate was disolved in water and the solution was washed successively with ethyl acetate and diethyl ether and then evaporated. To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure. The residue was recrystallized from isopropyl alcohol to give 1-[3-[4-(2-hydroxy-ethyl)-1-piperazinyl]propyl]-1H-tetrazole -5-thiol (1.35 g). N.M.R. (D20, d): 2.0-3.0 (14H, m), 3.79 (2H, t, J = 7Hz), 4.40 (2H, t, J =IT9z) . Preparation 19 (1) To the solution of N-(3-bromopropyl)phthalimide (53.0 g) in acetone (400 mi) were added. l-eçhoxvcarbonyl piperazine (50.0 g) and potassium carbonate (82.0 g). The resulting mixture was refluxed with stirring for 15 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with acetone. The filtrate and washing were combined and evaporated under reduced pressure. The residue was recrystallized from a mixture of benzene and n-hexane to give N-[3-(4-ethoxycarbonyl-1-piperatzinyl)propyl]phthalimide (65.2 g), mp 77 to 840C. (2) To z solution of N-[3-(4-ethoxycarbonyl-l-piperazinyl) propyl]phthalimide (23.9 g) in ethanol (300 mQ) was added 100% hydrazine hydrate (8.4 g). The resulting mixture was refluxed with stirring for 4 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with ethanol. The filtrate and washing were combined and evaporated under reduced pressure. After the addition of an aqueous solution of sodium hydroxide to the residue, the mixture was extracted with chloroform The extract was washed with water, dried over magnesium sulfate and then evaporated under reduced pressure to give an oil of 3-(4-ethoxycarbonyl-l-piperazinyl) propylamine (10.9 g). N.M.R. (CDCt3, g): 1.27 (3H, t, J = 7Hz), 1.63 (2H, tt, J = 7Hz) , 2.3-2.6 (6H, m), 2.80 (2H, t, J = 7Hz), 3.4-3.7 (4H, m), 4.17 (2H, q, J = 7Hz). (3) Tc a solution of potassium hydroxide (2.9 g) in methanol (40 m ) was added 3-(4-ethoxycarbonyl-1-piperazinyl) propylamine (10.9 g) and thereto was added carbon disulfide (3.9 g) under ice cooling over a period of 5 minutes. The resulting mixture was stirred for 4 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water (40 mQ) and washed with diethyl ether. The washed aqueous layer was icecooled and thereto was added methyl iodide (7.2 g) with stirring. The resulting mixture was stirred for 2 hours under ice-cooling, and extracted with ethyl acetate. The extract was dried over magnesium sulfate and then evaporated under reduced pressure to give an oil of methyl N-[3 (4-ethoxy-carbonyl-l-piperazinyl) propyl) dithiocarbamate (11.7 g). (d) To a solution of methyl N-[3-(4-ethoxy-carbonyl-l- piperazinyl)proPyl]dithiocarb=,.ate (11.7 g) in a mixtureof water (40 mQ) and ethanol (30 mQ) was added sodium azide (3.3 g). The resulting mixture was refluxed with stirring for 4 hours. The reaction mixture was concentrated under reduced pressure. After the addition of water to the residue, the mixture was washed with diethyl ether and then evaporated. To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure. After the addion of a mixture of hydrochloric acid and ethanol to the residue, the resulting mixture as evaporated under reduced pressure. The residue was triturated with acetone to give crude crystals of the object compound (5.7 g), which were recrystallized from ethanol to give l-[3-(4-ethoxycarbonyl-l-piperazinyl) propyl]-lH-tetrazole-5-thiol hydrochloride (2.1 g), mp 192 to 1970C (dec.). N.M.R. (DMSO-d6, 6): 1.22 (3H, t, J = 7Hz), 2.1-2.5 (2H, m) , 3.0-3.5 (6H, m) , 3.5-3.9 (4 H, m), 4.13 (-2H, q, J = 7Hz), 4.37 (2H, t, J = 7Hz). Preparation 20 (1) To the solution of N-(3-bromopropyl)phthalimide (30.1 g) in acetone (200 m±) were added l-isopropyl piperazine (23.0 g) and potassium carbonate (41.4 g). The resulting mixture was refluxed with stirring for 4 hours. The reaction maxture was cooled and then filtered. The filter cake was washed with acetone. The filtrate and washing were combined and evaporated under reduced pressure to give an oil of N-[3-(4-isopropyl-l-piperazinyl) propyl]phthalimide (43.5 g). (2) To a solution of N-[3-(4-isopropyl-l-piperazinyl) propylgphthalimide (43.1 g) in ethanol (350 mQ) was added 100% hydrazine hydrate (11.2 g). The resulting mixture was refluxed with stirring for 3 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with ethanol. The filtrate and washing were combined and concentrated under reduced pressure. The residual oil was subjected to column chromatography (aluminum oxide), eluting with a mixture of chloroform and methanol (20:1). The fractions containing the object compound were collected and then evaporated under reduced pressure. The residual oil was distilled under reduced pressure to give 3- (4-isopropyl-l-piperazinyl) propylamine (15.0 g), (bp t5 mmHg/1500C). (3) To a solution of potassium hydroxide (4.9 g) in methanol (40 mQ) was added 3-(4-isopropyl-l-piperazinyl) propylamine (14,7 g) and thereto was added carbon disulfide (6.0 g) under ice cooling over a period of 20 minutes. The resulting mixture was stirred for 3.5 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil we dissolved in water (40 mQ) and washed with diethyl ether. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (11.3 g) with stirring over a period of about 5 minutes. The resulting mixture was stirred for 2 hours under icecooling extracted with chloroform. The extract was washed with water, dried over magnesium sulfate and then evaporated to give an oil of methyl N-E3-(4-isopropyl-1- piperazinyl)propyl]dithiocarbamate (15.6 g). (4) To a solution of methyl N-[3--isopropy1-l-piperazinyl)- propyl]dithiocarbamate (15.1 g) in a mixture of water (50 mQ) and ethanol (40 mQ) was added sodium azide (4.6 g). The resulting mixture was refluxed with stirring for 4.5 hours. The reaction mixture was concentrated under reduced pressure. The concentrate was washed successively with diethyl ether and ethyl acetate and then evaporated. To the residue was added ethanol and the mixture was filtered. The filtrate was evaporated under reduced pressure. After the addition of a little amount of mixture of hydrochloric acid and ethanol to the residual oil, the product was recrystallized from. methanol to give 1;[3-(4- isopropyl-l-piperazinyl)propyl]-lH-tetrazole-5-thiol dihydrochloride (3.6 g). N.M.R. (DMSO-d6, 6): 1.28 (6H, d, J = 6Hz), 2.1-2.5 (2H, m), 3.1-3.8 (3H, m), 3.58 (8H, s), t.33 (2H, t, J = 8Hz). Preparation 23. (1) To the solution of N-(3-bromopropyl)phthalimide (15.6 g) in acetone (150 m±) were added 1-phenvlpiperazine (10.4 g) and potassium carbonate (12.0 g). The resulting mixture was refluxed with stirring for 4.5 hours. The reaction mixture was cooled and then filtered. The filter cake was washed with acetone. The filtrate and washing were combined and evaporated under reduced pressure. The residue was recrystallized from ethanol to give an oil of N-[3-(4-phenyl-1-piperazinyl)propyl]phthalimide (18.3 g), mp 129 to 1340C. 2) To a solution of N-[3-(-phenyl-l-piperazinyl) propyl]phthalimide (18.3 g) in ethanol (300 m2) was added 100% hydrazine hydrate (5.3 g). The resulting mixture was refluxed with stirring for 2 hours. The reaction mixture was evaporated under reduced pressure. After the addition of dil. aqueous solution 0 of sodium hydroxide to the residue, the mixture was extracted with chloroform. The extract was washed with water, dried over magnesium sulfate and then evaporated under reduced pressure to give 3-(4-phenyl -l-piperazinyl) propylamine (11.2g). N.X.R. (CCt4 6): 1.47 (2H, broad s), 1.57 (2H, tt, J = 7Hz), 2.38 (2H, t, J = 7Hz), 2.72 (2H, t, J = 7Hz), 2.4 - 2.7 (4H, m), 3.0-3.2 (4H, m) , 6.6-7.4 (5H, m). (3) To a solution of potassium hydroxide (2.85 g) in methanol (25 mQ) was added 3-(4-phenyl-l-piperazinyl) propylamine (11.1 g) and thereto was added carbon disulfide (3.85 g) under ice cooling over a period of 10 minutes. The resulting mixture was stirred for 3 hours under ice cooling. The reaction mixture was evaporated under reduced pressure. The residual oil was dissolved in water (40 mi) and washed with diethyl ether. The washed aqueous layer was ice-cooled and thereto was added methyl iodide (7.2 g) with stirring. The resulting mixture was stirred for 2 hours under ice-cooling and extracted with ethyl acetate. The extract was washed with water, dried over magnesium sulfate and then evaporated to give methyl N- [3- (4-phenyl-l-piperazinyl) propyl) dithiocarbamate (12.9 g), mp 75 to 790C. (4) To a solution of methyl N-[3-(4-phenyl-l-piperazinyl) propyl] dithiocarbamate (12.9 g) in a mixture of water (20 m ) and ethanol (30 m ) was added sodium azide (3.6 g). The resulting mixture was refluxed with stirring for 4.5 hours. The reaction mixture was concentrated under reduced pressure. After the addition of water to the residue, the mixture was washed successively with ethyl acetate and diethyl ether and then evaporated. To the residue was added ethanol (100 m ) and the mixture was filtered. The filtrate was evaporated under reduced pressure. The residue was recrystallized from isopropyl alcohol twice to give 1-[3-(4-phenyl-1-piperazinyl)propyl]-4,5-dihydro- lH-tetrazole-5-thione (3.2 g), mp. 200 to 2050C. IR. (Nujol): 3270, 1600 cm N.M.P. (DMSO-d6, 6): 1.8-2.1 (2E, m), 3.0-3.2 (4H, m), 4.17 (2H, t, J = 6Hz), 6.8-7.5 (5H, m). A solution of the above obtained product in a little amount of water was acidified with dil hydrochloric acid and'then adjusted to pH 7.0 with an aqueous solution of sodium bicarbonate. The precipitates were collected by filtration, washed with water and dried to give 1-[3-(4phenyl-1-piperazinyl)propyl]-1H-tetrazole-5-thiol, mp 220 to 2330C (dec.). I.R. (Nujol): 2350, 1590 cm N.M.R. (DMSO-d6, 6): 2.0-2.3 (2H, m), 4.3 (2H, t, J = 6Hz), 6.8-7.5 (5H, m) Preparation 22 The following conpounds were prepared according to the similar manners to those of Preparationl5-2l (1) 1-[2-(4-Methyl-1-piperatzinyl)ethyl]-1H-tetrazole 5-thiol hydrobromide, mp 216 to 2220C, N.M.P. (D20, 6): 2.80 (3H, s), 2.80 (4H, m), 3.04 (2H, t, J = 6Hz), 3.28 (4H, m), 4.48 (2H, t, J = 6Hz). (2) 1- (4-Methyl-l-piperazinyl) carbonylmethyl) -lH-tetrazole -5-thiol, mp > 2500C. Elemental Analysis: C H N Calcd 39.65 5.82 34.69 Found 39.33 5.68 34.52 Preparation 23 (1) A mixture of 4-(2-hydroxyethyl) thiosemicarbazide (10.3 g), benzyl chloride (10.6'g), water (30 mQ) and methanol (60 mQ) was stirred for 20 hours at room temperature to give a homogeneous solution containing benzyl N-(2-hydroxyethyl) thiocarbazimidate. The solution was ice-cooled and thereto was little by little added sodium nitrite (5.24 g) with sting and then dropwise added conc. hydrochloric acid (3.5 mi) over a period of 30 mimetes. The resulting mixture was stirred for an hour under ice-cooling. To the reaction mixture were added potassium carbonate (18 g) and water (30 m±). The mixture was stirred and then concentrated under reduced pressure. The concentrate was extracted with chloroform and the extract was washed with water, dried over magnesium sulfate and then evaporated under reduced pressure. The residual oil was subjected to column chromatography on silica gel. The fractions containing the object compound were collected to give an oil of 1-(2-hydroxyethyl)-5-benzylthio-1H-tetrazole (12.1 g). I.R. (Film): 3380, 1595, 1490 cm N.M.R. (C3CQ3,6): 3.1 (lH, m), 4.1 (2H, m), 4.23 (2H, m), 4.50 (2H, s), 7.33 (5H, s). (2) To a solution of l-(2-hydroxyethyl)-5-benzylthio- lH-tetrazole (7.0 g) in pyridine (50 mQ) was added p-toluenesulfonyl chloride (6.3 g) and the mixture was stirred for .3 hours. After the addition of water, the reaction mixture was extracted with ethyl acetate. The extract was washed with water, dried over magnesium sulfate and then concentrated under reduced pressure. To the residual oil containing l-(2-tosyloxyethyl)-5benzylthio-lH-tetrazole were added l-methylpiperazine (12.0 g), potassium carbonate (12.2 g) and N.N-dimethyl- formamide (55 m ) and the mixture was stirred for 5 hours at 500C. After the addition of water, the reaction mixture was extracted with ethyl acetate. The extract was washed with water, dried over magnesium sulfate and evaporated under reduced pressure. The crude product was subjected to column chromatography on silica gel. The fractions containing the object compound was collected to give an oil of l-[2-(4-methyl-1-piperazinyl)ethyl]- 5-benzylthio-lH-tetrazole (6.5 g). I.R. (Film): 1600, 1498, 1460, 1164 cm N.M.R. (CDCl3, d): 2.23 (3H, s), 2.1-2.7 (8H, m), 2.7 (2H, t, J = 6.0Hz), 4.17 (2H, t, J = 6.0Hz), 4.5 (2H, s), 7.27 (5H, s). 3) A mixture of 1-[2-(4-methyl-1-piperazinyl)ethyl]-5 benzylthio-lH-tetrazole (5.4 g), acetic acid (40 m) and conc. hydrobromic acid (40 mt was heated at 70 to 800C for 8 hours. After the concentration of the reaction mixture, the concentrate was diluted with water. The mixture was washed with diethyl ether twice and evaporated under reduced pressure. The residue was recrystallized from methanol. A solution of the obtained crystals in a little amounted of water was adjusted to pH 6.0 with 1N aqueous solution of potassium hydroxide., washed with ethyl acetate and then evaporated under reduced pressure. To the residue was added hot ethanol and insoluble materials were filtered out. The filtrate was cooled and the precipitated crystals were collected by filtration to give 1-[2-(4-methyl-1-piperazinyl)ethyl]-lH-tetrazole-5-thiol hydrobromide (1.1 g), mp 216 to 2220C. N.M.R. (D2 0, o) : 2.80 (3H, s), 280 (4H. m), 3.04 (2H, t, J = 6Hz), 3.28 (4H, m) , 4.48 (2H, t, J = óHz). Preparation 24 The following compounds were prepared according to the similar manner to that of Preparation 23. (1) 1-[3-(4-Methl-l-piperazinyl)propyl]-lH-tetrazole- 5-thioldihydrochloride, mp 239 to 243 C. (2) 1- [3- (4-Benzyl-l-piperazinyl) propyl) -lH-tetrazole-5- thiol dihydrochloride, mp 234 to 237 C. (3) 1-[3-(4-Allyl-1-piperazinyl)propyl]-1H-tetrazole-5 thiol-dihydrochloride, mp 211 to 21500 (dec.) (4) 1-[3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl]-1Htetrazole-5-thiol. N.M.R. (D20, 6): 2.0-3.0 (14H, m), 3.79 (2H, t, J = 7Hz), 4.40 (2H, t, J = 7Hz). (5) 1-[3-(4-Ethoxycarbonyl-1-piperazinyl)propyl]-1Htetrazole-5-thiol, mp 192 to 19700 (dec.). (6) 1-[3-(4-Isopropyl-1-piperazinyl)propyl]-1H-tetrazole 5-thiol dihydrochloride. NM.R. (DMSO-d6): 1.28 (6H, d, J = 6Hz), 2.1-2.5 (2H, m), 3.1-3.8 (3H, m), 3.58 (8H, s), 4.33 (2H, t, J = 8Hz) (7) 1-[3-(4-Phenyl-1-piperazinyl)propyl]-1H-tetrazole5-thiol, mp 220 to 223 C (dec.). (8) 1- (4-Methyl-l-piperazinyl) carbonylmethyl-lH-tetrazole5-thiol, mp > 250 C. Preparation 25 To a solution of l-carboxymethyl-lH-tetrazole-5- thiol (3.2 g) in dry acetone (60 mZ) was added triethyl amine (4.2 g). After the addition of a solution of pivaloyl chloride (4.3 g) in dry acetone (10 mQ) with stirring at -150C over a period of 5 minutes, the mixture was stirred for one hour and 15 minutes at the same temperature, Absolution of l-methylpiperazine (4.0 g) in dry acetone (10 mi) was dropwise added over a period of 5 minutes and the resulting mixture was stirred at -150C for 40 minutes and under ice-cooling for a further 2.5 hours. After the evaporation of the reaction mixture under reduced pressure, water was added to the residue, 4N aqueous solution of sodium hydroxide was added until the crystals were dissolved. The resulting solution was evaporated under reduced pressure and the residue was crystallized from water to give l-(4-methyl-l-piperazinyl) carbonylmethyl-lH-tetrazole-5-thiol (2.2 g). mp > 2500C. Elemental Analysis: C H N Calcd. 39.65 5.82 34.69 Found. 39.33 5.68 34.52 Preparation 26 (1) To a solution of benzhydryl 2-carboxy-2-(4-benzyloxyphemyl) acetate (10 g) in methylene chloride (70 ml) precooled at 0 C was added oxalyl chloride (1.54 mi) and the mixture was stirred for 30 minutes at OOC. The resulting mixture was dropwise added to a mixture of benzhydryl 7 &alpha;-methoxy-7ss-amino-cephalosporanate (8.5 g), pyridine (1.46 ml) and methylene chloride (80 mQ) over a period of about 2 minutes keeping the temperature below 40C and then the mixture was stirred for 1.5 hours at OOC. The reaction mixture was concentrated and to the concentrate were added water and ethyl acetate. The ethyl acetate layer was separated and thereto was added water. The mixture was adjusted to pH 1 with dil. hydrochloric acid and to the separated ethyl acetate layer was added water. The resulting mixture was adjusted to pH 8 with an aqueous solution of sodium bicarbonate. The ethyl acetate layer was separated, washed with a saturated aqueous solution of sodium chloride dried over magnesium sulfate and then filtered. The filtrate was evaporated to give a dark brown solid (15.7 g). A solution of the solid in methylene chloride was subjected to column chromatography (silica gel: 235 g), eluting with methylene chloride The fractions containing the object compound were collected and then concentrated. The residual oil was pulverized in diisopropyl ether to give a powder of benzhydryl 7cr-methoxy-7f3- [2-benzhydryloxy- carbonyl-2-4-(4-benzyloxyphenyl) acetamido) cephalosporanate (4.0 g) I.R. (Nujol): 3350, 1780, 1740, 1610, 1510 cm N.M.R. (DMSO-d6, #): : 1.98 (3H, s) , 3.30 (3H, s) 3.42 (2H, broad s), 4.6-4.9 (2H, broad s), 5.13 (3H, s), 5.24 (1H, s), 6.8-7.6 (31H, m). 2) To a solution of benzhydryl 7&alpha;-methoxy-7ss-[2-benzhy- dryloxycarbonyl-2-(4-benzyloxyphenyl)acetamido]cephalo sporanate (1.5 g), anisole (3.6 m±) in methylene chloride (15 mQ) was dropwise added a solution of aluminum chloride (2.21 g) in methyl nitrite (13 mQ) over a period of 5 minutes and the mixture was stirred for 1 hour and 40 minutes under ice-cooling. After the addition of water and ethyl acetate to there action mixture, the ethyl acetate layer was separated and thereto was added water. The mixture was adjusted to pH7 with an aqueous solution of sodium bicarbonate and then poured into water. The aqueous layer was separated and thereto was added ethyl acetate. The mixture was adjusted to pH1 with dil. hydrochloric acid and the ethyl acetate layer was separated, dried over magnesium sulfate and then filtered. The filtrate was concentrated and the concentrate was pulverized in diisopropyl ether to give 7&alpha;-methoxy-7ss- [D,Th-2-carboxy-2- ( 4-hydroxyphenyl) acetamido] cephalo- sporanic acid (0.7 g). I.R. (KBr): 3250, 3000, 1770, 1720, 1610, 1510, 1440, 1380 cm 1 N.M.R. (DMSO-d6, d): 2.0 (3H, s), 3.42 (3H, s), 3.3-3.6 (2H, m) , 4.70 (1H, s) , 4.6-4.9 (2H, m) 5.07 (lH, s), 6.67, 7.13 (4H, ABq, J = 8Hz), 9.4 (1H, broad s) Example 1 To a suspension of 7-aminocephalosporanic acid (12.1 g) and 1-(2-carboxy-2-hydroxyiminoethyl)-1H- tetrazole-5-thiol (9.0 g) in dry acetonitril (60 ml) was added boron trifluoride etherate (25.1 g) and the resulting solution was stirred at 50 for 50 minutes. Water (60 ml) was added and the solution was adjusted to pH 3.5 with conc. aqueous ammonia to give precipitates which were filtered, washed successively Kith water and acetone, and air-dried to give 7-amino-3 [1-(2-carboxy-2-hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (13.92 g). I.R. (Nujol) : 3160, 1800, 1620 cm N.N.R. (DMSO-d6, 6) : 3.62 (2H, m), 4.30 (2H, q, J=14,0 Hz), 4.77 (lH, d, J=5.OHz), 4.96 (1H, d, J=5.OHz), 5.24 (2H, broad s) Example 2 To a suspension of 7-aminocephalosporanic acid (0.9 g) and 1-(2-carboxy-2-methoxyiminoethyl)-1H- tetrazole-5-thiol (0.95 g) in dry acetonitrile (10 ml) was added boron trifluoride etherate(l.55 g) and the resulting solution was stirred at 500C for 2 hours. Water (10 ml) was added to the reaction mixture and the solution was adjusted to pH 3.5 with conc. aqueous ammonia under ice-cooling to give precipitates which were filtered, washed with water and dried to give 7-amino-3-[1-(2-carboxy-2-methoxyiminoethyl)-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (1.0 g), mp 170 to 1800C (dec.). I.R. (Nujol) : 1800, 1705, 1615 cm-1 N.M.R. (DMSO-d6, 6) : 3.63 (2H, ABq, J=18Hz), 3.96 (3H, s), 4.30 (2H, ABq, J=l4Hz), 4.78 (1H, d, J=5Hz), 4.97 (1H, d, J=5Hz), 5.27 (2H, s) Example 3 To a suspension of 7-[2-methoxyimino-2-(2aminothiazol-4-yl)acetamido]cephalosporanic acid (syn isomer) (1.14 g) and l-(2-carboxy-2-methoxyimino- ethyl)-lH-tetrazole-5-thiol (0.81 g) in water (20 ml) was added sodium bicarbonate and the solution was adjusted to pH 6.5 and then stirred for 5 hours at 60 C. After cooling at 10 to 150C, the solution was adjusted to pH 2.5 with 10% hydrochloric acid. The precipitates were collected by filtration, washed with water and then dried to give 7-[2-methoxyimino2-(2-aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl)-lH-tetrazol -5-yl)thiomethyl-3- cephem-4-carboxylic acid (syn isomer: sin the 7-position) I.R. (Nujol) : 3270, 1770, 1645 cm 1 Example d To a solution of 1-[3-(4-methyl-1-piperazinyl)propyl]- 1H-tetrazole-5-thioldihydrochloide (75.32 g) in water (6 Q) was added 7-aminocephalosporanic acid (60.0 g) and the mixture was adjusted to pH 5.4 with a saturated aqueous solution of sodium bicarbonate and stirred for 2 hours at 640C. The reaction mixture was cooled and adjusted to pH 6.84 with a saturated aqueous solution of sodium bicarbonate. The resulting mixture was subjected to column chromatography (CM-Cephadex, H-type: 3 b), eluting with water, 0.5% aqueous solution of sodium chloride and 2% aqueous solution of sodium chloride. The fiactions containing the object compound were collected. The resulting mixture (about 7Q) was adjusted to pH 4.5 with a saturated aqueous solution of sodium bicarbonate and then subjected to column chromatography (Non-ion adsorption resin, Diaion HP 20 prepared by Mitsubishi Chemical Industries: 3 #) and eluting with water, 2% isopropyl alcohol and 15% isopropyl alcohol. The fractions containing the object compound were collected and then concentrated. The concentrate was lyophilized to give a powder of 7-amino-3-[l-[3-(4-methyl-l-piperazinyl) propyl] -lH-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (34.6 g). IR. (KBr): 3400, 1760, 1600 cm N.M.R. (D2O, ); 2.30 (2H, m), 2.5-3.5 (lox, m), 2.90 (3H, s), 3.57, 3.83 (2H, ABq, J = 18Hz), 4.20, 4.40 (2H, ABq, J = 14Hz), 4.52 (2H, t, J = 7Hz), 4.88 (lH, d, J = 5Hz), 5.13 (lH, d, J = 5Hz). Example 5 A solution of 7-[2-methoxyimino-2-(2-aminothizol-4 yl)acetamido] cephalosporanic acid (syn isomer) (20.0 g) and l-[3-(4-methyl-1-piperazinyl)propyl]-lH-tetrazole-5 -thiolXdihydrochloride (16.6 g) in water (1Q) was adjusted to pH 6.0 with a saturated aqueous solution of sodium bicarbonate. The mixture was stirred for 3 hours and 20 minutes at 63 to 650C whiLe the pH was maintained at 6.0 to 6.2 with 5% hydrochloric acid. The reaction mixture was ice-cooled and adjusted to pH 7.3 with a saturated aqueous solution of sodium bicarbonate and thereto was added cold water (3 Q). The resulting solution was subjected to column chromatograph (CM-Cephadex, H-type: 1 Q), eluting with water (200 ml), 1% (2 ±) , 2% (2 Q) and 4% (4 ±) aqueous solution of sodium chloride The fractions containing the object compound were collected. Theresulting mixture (about 2l) was adjusted to pH 4.5 with a saturated aqueous solution of sodium bicarbonate and subjected to column chromatography (Non ion adsorption resin, Diaion HP 20 prepared by Mitsubishi Chemical Industries: 500 ml), eluting with water (0.5f), 5% (1l) and 15% (2.5 Q) isopropyl alcohol The fractions containing the object compound were collected and then concentrated The concentrate was lyophilized to give 7-[2-methoxyimino 2-(2-aminothiazol-4-yl)acetamido]-3-[1-[3-(4-methyl-1- piperazinyl)propyl]-1H-tetrazol-5-yl]thiomethyl-3-cephem4-carboxylic acid (syn isomer). (9.2 g). I.R. (Nujol): 3300, 1760, 1660, 1600, 1530 cm N.M.R. (DMSO-d6, 6): 2.0 (2H, m), 2.3-3.0 (l0H, m), 2.55 (3H, s), 3.47, 3.73 (2H, ABq J = 18Hz), 3.84 (3H, s), 4.32 (4H, broad s), 5.05 (lH, d, J = 5Hz), 5.67 (lH, dd, J = 5 and 8 Hz), 6.75 (1H, s), 7.22 (2H, broad s), 9.51 (lH, d, J = 8Hz). Example 6 A mixture of 7-[2-methoxyimino-2-(2-aminothiazol- 4-yl)acetamido]cephalosporanic acid (syn isomer) (1.2 g), 1-E2-(4-methyl-1-piperazinyl)ethyl]-lH-tetrazole-5- thiolhydrobromide (1.2 g) and phosphate buffer (PH6.4: 100 ml) was adjusted to pH 6.7 with a saturated aqueous solution of sodium bicarbonate. The mixture was stirred for 6 hours at 650C, cooled to room temperature and then adjusted to pH 4.5 with 5% hydrochloric acid. The resulting mixture was subjected to column chromatography (Non-ion adsorption resin, Diaion HP20 prepared by -?4itsubishi Chemical Industries: 60 ml), eluting with water, 5%, 10% and 20% isopropyl alcohol. The fractions containing the object compound were collected, and then concentrated. The concentrate was lyophilized to give powder of 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[2-(4-methyl-1-piperazinyl3ethyl3-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer) (0.70 g). I.R. (Nujol): 3300, 1765, 1660, 1600, 1530 cm 1 N.M.R. (DMSO-d6, 6): 2.5-3.1 (lOH, m), 2.60 (3H, s), 3.63 (2H, broad s), 3.87 (3H, s), 4.t7 (4H, broad s), 5.03 (lR,i, J = 5Hz), 5.70 (1H, dd, J = 5 and 8Hz), 6.77 (1H, s), 7.23 (2H, broad s), 9.56 (1H, i, J = 8Hz). Example 7. A mixture of 7-[2-methoxyimino-2-(2-aminothiazol-4- yl)acetamido]cephalosporanic acid (syn isomer) (1.5 g), 1-(4-methyl-1-piperazinyl)carbonylmethyl-lH-tetrazole-5- thiol (1.03 g), phosphate buffer (pH 6.4, 50 m±) and water (50 ml) was adjusted to pH 7.0 with a saturated aqueous solution of sodium bicarbonate and the mixture was stirred for 5 hours and 50 minutes at 600C. The reaction mixture was cooled and adjusted to pH 4.5 with 5% hydrochloric acid. The resulting mixture was subjected to column chromatography (Non-ion adsorption resin, Diaion HP20 prepared by Mitsubishi Chemical Industries: 75 mQ), eluting with water, 5% and 10% isopropyl alcohol. The fractions containing the object compound were collected and concentrated. The concentrate was lyophilized to give a powder of 7-12-methoxyimino-2-(2-aminqthiazol-4- yl)acetamido]-3-[1-(4-methyl-1-piperazinyl)carbonylmethyl -1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer). I.R. (Nujol): 3300, 1760, 1660, 1600, 1530 cm 1 N.M.R. (DMSO-d6, 6): 2.5 (3H, s), 2ç5-3.0 (4H. m), 3.4-3.9 (6H, m) , 3.82 (3H, s), 4.27 (2H, broad s), 5.08 (1H, d, J = 5Hz), 5.57 (2H, broad s), 5.65 (lH, dd, J = 5 and 8 Hz), 6.Z5 (1H, s), 7.17 (2H, broad s), 9.55 (1H, d, J = 5Hz). Example 8 A solution of 7&alpha;-methoxy-7ss-[D, L-2-carboxy-2-(4- hydroxyphenyl)acetamido]cephalosporanic acid (0.28 g), 1-[3-(4-methyl-1-piperazinyl)propyl]-lH-tetrazole-5- thiol dihydrochloride (1.3 g! in phosphate buffer (pH 6.4: 100 mZ) was stirred for 2 hours and 50 minutes at 650C. The reaction mixture was adjusted to pH 4.5 with 6N hydrochloric acid and then subjected to column chromatography (Non-ion adsorption resin, Diaion HP-20 prepared by Mitsubishi Chemical Industries: 50 mQ), eluting with water (50 ml), 3% (100 ml), 4% (100 mQ) and 25% (200 ml) isopropyl alcohol. The fractions containing the object compound were collected and concentrated. The concentrate was lyophilized to give a powder of 7&alpha;-methoxy-7ss-[D, L-2- carboxy-2-(4-hydroxyphenyl)acetamido]-3-[1-[3-(4-methyl1-piperatinyl)propyl]-1H-tetrazol-5-yl]thiomethyl-3cephem-4-carboxylic acid (0.15 g). I.R. (Nujol): 3200, 1760, 1670, 1600, 1505 cm 1 N.M.R. (D20-NaHC03, 6): 2.5-3.2 (12H, m), 2.75 (3H, s), 3.5 (3H, s), 3.6 (2X, broad s), 4.2-4.6 (4H, m), 5.13, 5.16 (1H, each s), 5.35 (1H, s), 6.97, 7.40 (4H, ABq, J = 8Hz). Example 9 The following compounds were prepared according to the similar manners to those of Examples 1 to 8. (1) 7-amino-3-[1-(2-carboxy-2-allyloxyiminoethyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid, mp 165 to 170 C (dec.). I.R. (Nujol) : 1790, 1695, 1605 cm N.M.R. (DMSO-d6, 6) : 3.60 (2H, ABq, J=18Hz), 4.30 (2H,,ABq, J=l4Hz), 4.67-5.10 (6H, m), 5.29 (2H, s), 5.60-6.23 (1H, m) (2) 7-amino-3-[1-(2-carboxy-2-hydroxyethyl)-1H tetrazol-5-yl]thiomethylf-3-cephem-4-carboxylic acid. I.R. (Nujol) : 1795, 1725, 1615 cm N.M.R. (DMSO-d6, #) : 3.67 (2H, m), 4.23-4.73 (5H, m), 4.91 (1H, d, J=5Hz), 5.10 (1H, d, J=5Hz) (3) 7-[2-Methoxyimino-2-(2-formamidothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.H. (Nujol) : 1760, 1650 cm 1 (4) 7-[2-Cyclopentyloxyimino-2-{2-(2,Z,2-trifluoro- acetamido)thiazol-4-yl)acetamido]-3-[1-(2-carboxy 2-hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl s-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.H. (Nujol) : 3150, 1770, 1710,1660 cm (5) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2- formamidothiazol-4-yl)acetamido]-3-[1-(2 carboxy-2-hydroxyiminoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3200, 1780, 1660 cm 1 (6) 7-[2-(4-Fluorobenzyl)oxyimino-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3 [1-(2-carboxy-2-hydroxyiminoethyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3300, 1780, 1720, 1670 cm 1 (7) 7-t2-Methoxyimino-2-{2-(2,2,2-trifluoroacetamido)- thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 164 to 1700C (dec.). I.R. (Nujol) : 1780, 1715, 1655 cm (8) 7-[2-Isopropoxyimino-2-(2-(2,2,2- trifluoroacetamido)thiazol-4-yl}acetamido]-3 [1-(2-carboxy-2-methoxyiminoethyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). -l I.R. (Nujol) : 1780, 1715 cm (9) 7-[2-Allyloxyimino-2-(2-formamidothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1770, 1670 cam 1 (10) 7-[2-(2-Propynyloxyimino)-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl}acetamido]-3-[1 (2-carboxy-2-methoxyiminoethyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3225, 2110, 1770, 1715, 1670 cm lz (11) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 formamidothiazol-4-yl)acetamido]-3-[1-(2-carboxy 2-methoxyiminoethyl)-lH-tetrazol -5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 156 to 161 C (dec.). -l I.R. (Nujol) : 1770, 1665 cm (12) 7-[2-Benzyloxyimino-2-{2-(2, 2, 2-trifluoroacetamido) thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl) -lH-tetrazol --5-yI)thiomethyl- 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 165 to 1700C (dec.). I.R. (Nujol) : 1775,' 1710, 1650 cm 1 (13) 7-[2-(4-Fluorobenzyl)oxyimino-2-{2-(2, 2, 2 trifluoroacetamido)thiazol-4-yl}acetamido]-3 [1-(2-carboxy-2-methoxyiminoethyl)-1H-tetrazole 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 153 C (dec.). I.R. (Nujol) : 1765, 1710, 1650 cm 1 (14) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 formamidothiazol-4-yl)acetamido]-3-[1-(2-carboxy- 2-allyloxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 150 to 1600C (dec.). I.R. (Nujol) : 1775, 1670 cm (15) 7-[2-Benzyloxyimino-2-{2-(2,2,2-trifluoroacetamido) thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2 allyloxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1775, 1710, 1650 cm 1 (16) 7-[2-(4-Fluorobenzyl)oxyimino-2-{2-(2, 2, 2 trifluoroacetamido)thiayol-4-yl}acetamido]-3 [1-(2-carboxy-2-hydroxyethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 90 to 100 C (dec.). I.R. (Nujol) : 1770, 1710, 1650 cm 1 (17) 7-[2-Methoxyimio-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 169 to 1730C (dec.). I.R. (Nujol) : 3300, 3200, 1770, 1660, 1630 cm (18) 7-[2-Cyclopentyloxyimino-2-(2-aminothiazol-4-yl)- acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl)- lH-tetrazol -5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 1710C (dec.). I.R. (Nujol) : 3250, 3150, 1760, 1650 cm (19) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2-aminothiazol- 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 150 to 160 C (dec.). I.H. (Nujol) : 3270, 3160, 1770, 1650 cm 1 (20) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyimino- ethyl)-lH-tetrazol -5-yl]thiomethyl-3-cephem-4- carboxylic acid (syn isomer: in the 7-position), mp 160 to 1630C.(dec.). I.R. (Nujol) : 3200, 1770, 1640 cm (21) 7-[2-Isopropoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3260, 1770, 1660 cm 1 (22) 7-{2-Allyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 130 to 1400C (dec.). I.H. (Nujol) : 3250, 1770, 1650 cm 1 (25) 7-[2-(2-Propynyloxyimino)-2-(2-aminothiazol-4- yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3255, 1775, 1670, 1630 cm 1 (24) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3260, 1770, 1650 cm (25) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 172 C (dec.). I.R. (Nujol) : 3260, 1770, 1655 cm 1 (26) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyimino ethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4 carboxylic acid (syn isomer: in the 7-position), mp 130 to 1400C (dec.). I.R. (Nujol) : 3270, 1770, 1625 cm 1 (27) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 allyloxyiminoethyl)-lH-tetrazol-5-yl]thiomethyl- 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 150 C (dec.). I.R. (Nujol) : 3270, 1770, 1660, 1630 cm 1 (28) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-allyloxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 180 to 190 C (dec.). I.R. (Nujol) : 3290, 1775, 1705, 1655 cm 1 (29) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1765, 1630 cm 1 (30) Disodium 7-amino-3-[1-(2-carboxylate-2-tert butoxycarboxaminoethyl) -lH-tetrazol.-S-yl) thiomethyl-3-cephem-4-carboxylate. I.R. (Nujol) : 1750, 1685, 1600 cm 1 N.M.H. (D20, 6) : 1.30 (9H, s), 3.70 (2H, ABq, J=17Hz), 4.05-4.87 (6H, m), 5.04 (1H, d, J=SHz) (31) 7-[2-Methoxyimino-2-{2-(2,2,2-trifluoroacetamido)- thiazol-4-yl)acetamido]-3-[l-(2-carboxy- hydroxyethyl)-lH-tetrazol -5-yl]thiomethyl-3 cephem-4-carboxylic acid (syn'isomer: in the 7-position), mp > 1400C (dec.). -l I.H. (Nujol) : 3180, 1765, 1715, 1650 cm (32) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyethyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3320, 1770, 1635 cm 1 (33) 7-[2-Methoxyimino-2-{2-(2, 2, 2-trifluoroacetamido) thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2-tert butoxycarboxamidoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer in the 7-position), mp 189 to 1960C (dec.). I.R. (Nujol) : 1780, 1695 cm (34) 7-[2-Blethoxyimino-2-{2-(2,2,2-trifluoroacetamido)- thiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 aminoethyl)-lH-tetrazol -S-yl)thiomethyl-3-cephem- 4-carboxylic acid formate (syn isomer: in the 7-position). I.R. (Nujol) : 3160, 1760, '1650 cm 1 (35) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-(2-carboxy-2-aminoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1765, 1640 cm (36) 7-Amino-3-[1-(3-carboxy-1-propenyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid. I.R. (Nujol) : 1795 cm N.M.R. (DMSO-d6, 6) : 3.35 (2H, m), 3.63 (2H, ABq, J=18Hz), 4.33 (2H, ABq, J=14Hz), 4.75 (1H, d, J=SHz), 4.94 (1H, d, J=5Hz), 6.18 (1H, m), 6.96 (1H, m) (37) 7-Amino-3-[1-(1-carboxyallyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid. I.R. (Nujol) : 1795 cm 1 N.M.R. (DMSO-d6, 6) : 3.57 (2H, m), 4.32 (2H, m), 4.84 (2H, m), 5.18-5.40 (2H, m), 5.80-6.47 (2H, m) (38) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3300, 1760, 1660 cm-1 (39) 7-[2-(2-Propynyloxyimino)-2-(2-aminothiazol-4-yl) acetamido]-3-[lt > -carboxy-l-propenyl)-lH-tetrazol- 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3320, 2150, 1775, 1675 cm 1 (40) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(1-carboxyallyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3350, 1765, 1660, 1605 cm 1 (41) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2- - formamidothiazol-4-yl)acetamido]-3-[1-(3-carboxy l-propenyl)-lH-tetrazol-5-yl]thiomethyl-3-cephem- 4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1770, 1660 cm 1 (42) 7- [2-(2-Cyclopenten-1-yl)oxyimimno-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(3-carboxy-1 propenyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem 4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1770, 1660 cm 1 (43) 7-[2-Phenoxyimino-2-(2-formamidothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). N.M.R. (DMSO-d6, 6) : 3.33-3.90 (6H, m), 4.10-4.67 (2H, m), 5.23 (1H, d, J=SHz), 5.80-6.33 (2H, m), 6.90-7.57 (6H, m), 7.73 (1H, s), 8.60 (1H, s), 9.03 (lH, d, J=8Hz), 12.86 (lH, broad s) (44) 7-[2-Phenoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3150, 1770, 1650, 1585 cm 1 (45) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-isopropyl-1-piperazinyl)propyl] lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1660, 1600, 1530 cm 1 N.M.R. (DMSO-d6): 1.22 (6H, d, J = 6Hz), 2.0 (2H, m), 2.3-3.1 (llH, m), 3.87 (3H, s), 4.37 (4H, broad s), 5.10 (1H, d, j = 5Hz), 5.73 (1H, dd, J = 5 and 8 Hz), 6.77 (1H, s), 7.23 (2H, broad s), 9.57 1H, d, j,= 8Hz). (46) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-benzyl-1-piperazinyl)propyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. -1 I.R. (Nujol): 3300, 1760, 1660, 1600 cm 1 N.M.R. (DMSO-d6, Ï): 2.0 (2H, m), 2.5-2.8 (lOH, broad s), 3.60 (2H, broad s), 3.73 (2H, broad s), 3.86 (3H, s), 4.30 (4H, broad s), 5.10 (IH, d, J = SHz), 5.74 (1H, dd, J = 5 and 8 Hz), 6.77 (1H, s), 7.40 (7H, broad s), 9.56 (1H, d, J = 8Hz). (47) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-allyl-1-piperazinyl)propyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer) , powder. -l I.R. (Nujol): 3350, 1770, 1670, 1600, 1530 cm N.M.R. (DMSO-d6, 6): 3.83 (3H, s) , 5.00 (1H, d, J = 5Hz) , 6.73 (lH, s), 7.20 (2H, broad s), 9.50 (1H, d, J = 8H2). (48) Sodium 7-[2-methoxyimino-2-(2- aminothiazol-4-yl) acetamido]-3-[1-[3-(4-phenyl-1-piperazinyl)propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1665, 1600, 1530 cm N.M.R. (DMSO-d6, 6): 2.1 (2H, m), 2.5-3.4 (lOH, m), 3.63 (2H, broad s), 3.80 (3H-, s), 4.25 (2H, broad s), 4.33 (2H, broad s), 5.05 (1H, d, J = 5Hz), 5.70 (lH, dd, J= 5 and 8 Hz), 6.68 (lH, s), 6.7-7.3 (7H, m), 9.48 (1H, d, J = 8Hz). (49) 7- [2-methoxyimino-2- (2-aminothiazol-4-yl) acetamido] -3-[1-[3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol) : 3350, 3250, 1775, 1670, 1610, 1540 cm N.M.R. (DMSO-d6, 6): 1.9-2.1 (2H, m), 2.3-3.0 (12H, m), 3.62 (4H, broad s), 3.80 (3H, s), 4.30 (4H, broad s), 5.00 (1H, d, J = 5Hz), 5.62 (lH, dd, J = 5 and 8 Hz), 6.70 (1H, s), 7.16 (2H, broads), 9.44 (lH, d, J = 8Hz). (50) 7-[2-methoxyimino-2-(2-Ezino.hiazol-4-yl)acetamiCo]- 3-[1-[3-(4-ethoxycarbonyl-1-piperazinyl)propyl]-1H-tetrazol5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol) : 3300, 1770, 1670, 1600, 1530 cm N.M.R. (DMSO-d6, o): 1.17 (3E, t, J = 6.0Hz), 2.0(2H, m), 2.3-2.6(6H, m), 3.2-3.5 (4H, m), 3.67 (2H, broad s), 3.83 (3H, s), 4.03 (2H, q, J = 6Hz), 4.33 (4H, broad s), 5.13 (1H, d, J = 5Hz), 5.77 (1H, dd, J = 5 and 8 Hz), 6.78 (1H, s), 7.15 (2H, broad s), 9.50 (1H, d, J = 8Hz). (51) 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2-formamidothiazol -4-yl)acetamido]-3-[1-[3-(4-methyl-1-piperazinyl)propyl] -lM-tetrazol-5-yl]thiomethyl-3-cephem-4-caXboxylic acid (syn isomer), powder. I.R. (Nujol): 3350, 1775, 1670, 1600, 1550 cm (52) 7- [2- (2-cyclopenten-l-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-[3-(4-methyl-1-piperazinyl)propyl]- 1H-terazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1770, 1660, 1620, 1530 cm 1 Example 10 2- (2-Cyclopenten-l-yl)oxyimino-2- (2- formamidothiazol-4-yl)acetic acid (syn isomer)(1.00 g) was added at 0 C to vilsmeier reagent which had been prepared from N,N-dimethylformamide (0.30 g) and phosphoric chloride (0.62 g) in ethyl acetate (1.2 ml) and tetrahydrofuran (10 ml), and the mixture was stirred at the same temperature for 30 minutes to afford the activated solution. The activated solution was added dropwise at -5 to O C to a solution of 7-amino 3- C1-(2-carboxy-2 hydroxyiminoethyl) 1H-tetrazol -5yl]thiomethyl-3-cephem-4-carboxylic acid (1.30 g) in water (8 ml) and acetone (8 ml), adjusted to pH 8.0 with sodium bicarbonate and the mixture was stirred at the same temperature for 30 minutes. To the resultant mixture was added ethyl acetate (10 ml), and the aqueous phase was separated from the mixture. To the aqueous solution was added ethyl acetate (60 ml) and the mixture was adjusted to pH 2.0 with 10% hydrochloric acid with stirring, and the ethyl acetate layer was separated, washed with water, treated with activated carbon, dried over magnesium sulfate and evaporated. The residue was pulverized with diethyl ether to give 7-[2-(2cyclopenten-1-yl)oxylimino-2-(2-formamidothiazol-4 yl)acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position) (1.17 g). I.R. (Nujol) : 3200, 1780, 1660 cm 1 '.M.R. (DMSO-d6) : : 1.75-2.73 (4H, m), 3.73 (2H, m), 4.39 (2H, q, J=14.0Hz), 5.03-5.53 (4H, m), 5.62-6.36 (3H, m), 7.43 (1H, s), 8.56 (1H, s), 9.63 (lH, d, J=8.0Hz) Example 11 2-Methoxyimino-2-[2-(2,2,2-trifluoroacetamido) thiazol-4-yl]acetic acid (syn isomer)(0.97 g) was added at 0 C to vilsmeier reagent which had been prepared from N,N-dimethylfcrmamide (0.31 g) and phosphoryl -- chloride (0.65 g) in ethyl acetate (1.2 ml) and tetrahydrofuran (7.4 ml), and the mixture was stirred at the same temperature for 30 minutes to afford the activated solution. The activated solution was added. dropwise at -5 to O C to a solution of 7-amino-3-[1-(2- carboxy-2-methoxyiminoethyl)-lH-tetrazol -5-y1)- thiomethyl-3-cephem-4-carboxylic acid (1.40 g) in water (10 ml) and acetone (8 ml), adjusted to pH 8.0 with sodium bicarbonate and the mixture was stirred at the same temperature for 45 minutes. To the resultant mixture was added ethyl acetate (10 ml), and the aqueous phase was separated from the mixture. To the aqueous solution was added ethyl acetate (70 ml) and the mixture was adjusted to pH 2.0 with 10% hydrochloric acid with stirring, and the ethyl acetate layer was separated, washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and evaporated. The residue was pulverized with diisopropyl ether to give 7-r2-methoxyimino-2-(2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3-[1-(2carboxy-2-methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position) (1.77 g), mp 164 to 1700C (dec.). -l I.R. (Nujol) : 1780, 1715, 1655 N.M.R. (DMSO-d6, 6) : 3.70 (2H, m), 3.91 (3H, s), 3.97 (3H, s), 4.39 (2H, m), 5.14 (1H, d, J=4.5Hz), 5.27 (2H, s), 5.81 (lH, dd, J=4.5 and 8Hz), 7.52 (lH, s), 9.73 (lH, d, J=8Hz) Example 12 2-(2-Propynyloxyimino)-2-[2-(2,2,2 trifluoroacetamido)thiazol-4-yl]acetic acid (syn isomer) (1.05 g) was added at 0 C to vilsmeier reagent which had been prepared from N,N-dimethylformamide (0.31 g) and phosphoryl chloride (0.65 g) in ethyl acetate (1.2 ml) and tetrahydrofuran (7.4 ml), and the mixture was stirred at the same temperature for 30 minutes to afford the activated solution. The activated solution was added dropwise at -5 to 0 C to a solution of 7-amino 3-[1-(2-carboxy-2-methoxyiminoethyl)-1H-tetrazol-5yl]thiomethyl-3-cephem-4-carboxylic acid (1.40'g) in water (10 ml) and acetone (8 ml), adjusted to pH 8.0 with sodium bicarbonate and the mixture was stirred at the same temperature for 35 minutes. To the resultant mixture was added ethyl acetate (10 ml), and the aqueous layer was separated from the mixture. To the aqueous solution was added ethyl acetate (70 ml) and the mixture was adjusted to pH 1.8 with 10% hydrochlonic acid with stirring, and the ethyl acetate layer was separated, washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate and evaporated. The residue was pulverized with diisopropyl ether to give 7-[2-(2-propynyloxyimino)-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3-[1-(2carboxy-2-methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position)(2.05 g). I.R. (Nujol) : 3225, 2110, 1770, 1715, 1670 cm-1 N.M.R. (DMSO-d6, 6) : 3.47 (1H, m), 3.69 (2H, m), 3.97 (3H, s), 4.35 (2H, m), 4.77 (2H, m), 5.14 (1H, d, J=SHz), 5.26 (2H, s), 5.80 (lH, dd, J=5 and 8Hz), 7.55 (1H, s), 9.80 (1H, d, J=8Hz) Exemple 13 To a colution of N,N-dimethylformamido t277 mg) in tetrahydrofuran (1 ml) was dropwise added a solution of phosphorus oxychloride (583 mg) in tetrahydrofuran (9 mZ) at -10 C and the mixture was stirred at room temperature. After the addition of 2-(2-cyclopenten-lyl)oxyimino-2-(2-formamidothiazol-4-yl)acetic acid (syn isomer) (900 mg) at -10 C, the resulting mixture was stirred for 30 mines at 0 C and then the temperature was raised to 100C to give a solution (Solution A). On the other hand, a solution of 7-amino-3-[1-[3-(4-methyl-1- piperazinyl)propyl]-1H-tetrazol-5-yl] thiomethyl-3 cephem-4-carboxylic acid (1.2 g) in a mixture cf water (12 m±) and acetone (12 m±) was adjusted to pH 7.0 with 2 saturated aqueous solution of sodium bicarbonate and then cooled to OOC. To the solution was dropwise added te above obtained solution A over a period of 5 minutes while the pH was maintained at 7. The resulting solution was allowed to warm to room temperature, stirred or 1.5 hcurs, adjusted to pM 6.5 with dil, hydrochloric acid and then concentrated under reduced pressure. The precipitates were collected by filtration to give 7-[2-(2-cyclopenten1-yl)oxyimino-2-(2-formamidothiazol-4-yl)acetamido]-3-[1 [3-(4-methyl-1-piperazinyl)propyl]-1H-tetrazol-5-yl]thiomethyl 3-cephern-4-carboxylic acid (syn isomer) (300 mg). The filtrate was subjected to column chromatography (Mon-ion adsorption resin, Diaion HP20 prepared by Mitsubishi Chemical Industries, 60 mi, eluting with water, 5%, 15% and 25% isopropyl alcohol. The fractions containing the object compound were collected and concentrated. The concentrate was lyophilized to give the same object compound (0.85 g), powder. I.R. (Nujol) : 3350, 1775, 1670, 1600, 1550 cm-1 N.M.R. (DMSO-d6, #): 1.8-3.0 (19H, m), 3.6 (2H, broad s) 4.43 (4H, broad s), 5.07 (1H, d, J = 5Hz), 5.2-5.5 (lH, m), 5.6-6.2 (3H, m), 7.38 (1H, s), 8.53 (1H, s), 9.55 (1H, d, J= 8 H) Example 14 The following compounds were prepared according to the similar manners to those of examples lOtO 13 (1) 7-[2-Methoxyimino-2-(2-formamidothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1760, 1650 cm 1 N.M.R. (DMSO-d6, #) : 3.72 (2H, m), 3.90 (3H, s), 4.36 (2H, m), 5.04-5.46 (3H, m), 5.83 (1H, dd, J=4.0 and 8.0Hz), 7.43 (1H, s),. 8.55 (1H, s), 9.78 (1H, d, J=8.0Hz) (2) 7-[2-Cyclopentyloxyimino-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3 [1-(2-carboxy-2-hydroxyiminoethyl)-lH-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3150, 1770, 1710, 1660 cm 1 N.M.R. (DMSO-d6, #) : 1.31-2.01 (8H, m), 3.69 (2H, m), 4.35 (2H, m), 4.72 (1H, m), 5.14 (1H, d, J=4.0Hz), 5.27 (2H, s), 5.80 (lH, dd, J=4.0 and 8.0Hz), 7.48 (1H, s), 9.67 (1H, d, J=8.0Hz) (3) 7-[2-(4-Fluorobenzyl)oxyimino-2-{2-(2,2,2 trifluoroacetamido)thiazol-4-yl)acetamido]-3-[1-(2 (2-carboxy-2-hydroxyiminoethyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3300, 1780, 1720, 1670 cm N.M.R. (DMSO-d6, #) : 3.70 (2H, m), 4.41 \ (2H, m), 5.04-5.45 (SH, m), 5.87 (lH, dd, J=4.0 and 8.0Hz), 6.96-7.72 (4H, m), 7.58 (1H, s), 9.88 (1H, d, J=8. 0Hz) (4) 7-[2-Isopropoxyimino-2-{2-(2,2,2-trifluoroacetamido) thiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl)-lH-tetrazol-5-yl]thiomethyl-3- cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1780, 1715 cm 1 N.M.R. (DMSO-d6, 6) : 1.22 (3H, s), 1.33 (3H, s), 3.72 (2H, m), 3.98 (3H, s), 4.21-4.67 (3H, m) , 5.17 (1H, d, J=SHz) , 5.28 (2H,s), 5.83 (1H, dd, J=5 and 8Hz), 7.48 (1H, s), 9.68 (1h, d, J=8Hz) (5) 7-[2-Allyloxyimino-2-(2-:ormamidothiazol-4-yl)- acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (sn isomer: in the 7-position). I.R. (Nujol3 : 1770, 1670 cm-1 N.M.R. (DMSO-d6, 6) : 3.73 (2H, m), 4.02 (3H, s), 4.43 (2H, m), 4.68 (2H, m), 5.10-5.60 (5H, m), 5.77-6.30 (2H, m), 7.46 (1H, s), 8.57 (1H, s), 9.76 (lH, d, J=8Hz) (6) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 formamidothiazol-4-yl)acetamido]-3-[1-(2-carboxy 2-methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 156 to 1610C (dec.). I.R. (Nujol) : 1770, 1665 cm 1 N.M.R. (DMSO-d6, 6) : 1.70-2.60 (4H, m), 3.67 (2H, m), 3.98 (3H, s), 4.37 (2H, m), 5.02-5.46 (4H, m), 5.71-6.20 (3H, m), 7.39 (lH, s), 8.52 (lH, s), 9.60 (lH, d, J=8Hz) (7) 7-t)-Benzyloxyimino-2-{2-(2,2,2-trifluoroacetamido)- thiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 165 to 1700C (dec.). I.R. (Nujol) : 1775, 1710, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.67 (2H, m), 4.00 (3H, s), 4.37 (2H, m), 5.13-5.50 (5H, m), 5.91 (1H, dd, J=5 and 8Hz), 7.40 (5H, m), 7.57 (1H, s), 9.88 (1H, d, J=8Hz) (8) 7-[2-(4-Fluorobenzyl)oxyimino-2{2-(2,2,2 trifluoroacetamido)thiazol-4-yl)acetamido]-3 [1-(2-carboxy-2-methoxyiminoethyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position, (mp 1530C (dec.). I.R. (Nujol) : 1765, 1710, 1650 cm-1 N.M.R. (DMSO-d6, 6) : 3.65 (2H, m), 3.97 (3H, s), 4.37 (2H, m), 5.08-5.40 (SH, m), 5.83 (1H, dd, J=5 and 8Hz), 7.03-7.63 (5H, m), 9.88 (1H, d, J=8Hz) (9) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 formamidothiazol-4-yl)acetamido]-3-[1-(2-carboxy 2-allyloxyiminoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 150 to 1600C (dec.). I.R. (Nujol) : 1775, 1670 cm N.M.R. (DMSO-d6, 6) : 1.77-2.70 (4H, m), 3.70 (2H, m), 4.40 (2H, ABq, J=14Hz), 4.77 (2H, m), 5.03-5.50 (6H, m), 5.63-6.30 (4H, m), 7.40 (lH, s), 8.57 (1H, s), 9.62 (lH, d, J=8Hz) (10) 7-[2-Benzyloxyimino-2-{2-(2,2,2-trifluoro acetamido)thiazol-4-yl)acetamido]-3-[1-(2 carboxy-2-allyloxyiminoethyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1775, 1710, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.70 (2H, m), 4.30 (2M, m), 4.80 (2H, m), 5.07-5.60 (7H, m), 5.68-6.21 (2H, m), 7.42 (5H, m), 7.56 (1H, s), 9.88 (lH, d, J=8Hz) (11) 7-[2-(4-Flourobenzyl)oxyimino-2-{2-(2,2,2 trifluoroacetamido)thiazol-4-yl)acetamido]-3 [1-(2-carboxy-2-hydroxyethyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 90 to 100 C (dec.). I.R. (Nujol) : 1770, 1710, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.73 (2H, m), 4.20-4.80 (5H, m) , 5.10-5.47 (3H, m), 5.88 (lH, dd, J=5 and 8Hz), 6.88-7.70 (5H, m)s 9.89 (lH, d, J=8Hz) (12) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 169 to 1730C (dec.). I.R. (Nujol) : 3300, 3200, 1770, 1660, 1630 cm 1 (13) 7-[2-Cyclopentyloxyimino-2-(2-aminothiazol-4 yl)acetamido]-3-tl-(2-carboxy-2-hydroxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 171 C (dec.) I.R. (Nujol) : 3250, 3150, 1760, 1650 cm-1 (14) 7-[2-(2-cyclopenten-l-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 150 to 1600C (dec.). I.R. (Nujol) : 3270, 3160, 1770, 1650 cm-1 (15) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyimino ethyl)-lH-tetrazol -5-ylgthiomethyl-3-cephem-4- carboxylic acid (syn isomer: in the 7-position), mp 160 to 163 C. I.R. (Nujol) : 3200, 1770, 1640 cm 1 (16) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3270, 1770, 1645 cm 1 (17) 7-[2-Isopropoxyimino-2-(2-aminothazol-4yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3260, 1770, 1660 cm 1 (18) 7-[2-Allyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-t 2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 130 to 1400C (dec.). I.H. (Nujol) : 3250, 1770, 1650 cm 1 (19) 7-[2-(2-Propynyloxyimino)2-(2-aminothiazol-4 yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3255, 1775, 1670, 1630 cm 1 (20) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 methoxyiminoethyl)-lH-tetrazol -5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 15Q C (dec.). l.R. (Nujol) : 3260, 1770, 1650 cm 1 (21) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 172 C (dec.). I.R. (Nujol) : 3260, 1770, 1655 cm-1 (22) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]3-[1-(2-carboxy-2-methoxyiminoethyl) lH-tetrazol -5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 130 to 140 C (dec.). I.R. (Nujol) : 3270, 1770, 1625 cm-1 (23) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2- aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 allyloxyiminoethyl)-lH-etrazol -S-yl]thiomethyl- 3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3270, 1770, 1660, 1630 cm (24) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido-3-[1-(2-carboxy-2-allyloxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 180 to 190 C (dec.). I.R. (Nujol) : 3290, 1775, 1705, 1655 cm-1 (25) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1765, 1630 cm-1 (26) 7-[2-Methoxyimino-2-{2-(2,2,2-trifluoroacetamido) thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2 hydroxyethyl)-lH-tetrazol-5-yl]thiomethyl-3-cephem 4-carboxylic acid (syn isomer: in the 7-position), mpl40 C (dec.). I.R. (Nujol) : 3180, 1765, 1715, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.70 (2H, m), 3.91 (3H, s), 4.20-4.77 (5H, m), 5.13 (lH, d, J=5Hz), 5.75 (lH, dd, J=5 and 8Hz), 7.52 (1H, s), 9.75 (1H, d, J=8Hz) (27) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyethyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3320, 1770, 1635 cm 1 (28) 7-E2-Methoxyimino-2-{2-(2,2,2-trifluoroacetamido)- thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2-tert butoxycarboxamidoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 189 to 1960C (dec.). I.R. (Nujol) : 1780, 1695 cm N.M.R. (DMSO-d6, 6) : 1,30 (9H, s), 3.70 (2H, m), 3.89 (3H, s), 4.22-4.70 (SH, m), 5.12 (1H, d, J=5Hz), 5.80 (1H, dd, J=5 and 8Hz), 7.52 (1H, s), 9.73 (lH, d, J=8Hz) (29) 7-12-Methoxyimino-2-{2-(2,2,2-trifluoroacetamidor- thiazol-4-yl)acetamido]-3-[1-(2-carboxy-2 aminoethyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxylic acid formate (syn isomer: in the 7-position). I.R. (Nujol) : 160, 3160, 1760, 1650cm 1 (30) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-(2-carboxy-2-aminoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1765, 1640 cm (31) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3300, 1760, 1660 cm 1 N.M.R. (DMSO-d6, 6) : 3.63 (2H, m), 3.87 (3H, s), 4.40 (2H, ABq, J=14Hz), 5.03 (3H, m), 5.67 (2H, m), 6.30 (1H, m), 6.73 (1H, s), 7.27 (2H, broad s), 9.57 (1H, d, J=8Hz) (32) 7-[2-(2-Propynyloxyimino)-2-{2-(2,2,2 trifluoroacetamido)thiazol-4-yl)acetamido]-3 [1-(3-carboxy-1-propenyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.H. (Nujol) : 2120, 1780, 1720 cm-1 N.M.R. (DMSO-d#, d) : 3.47-3.70 (5H, m), 4.33-4.47 (2H, m), 4.80 (2H, m), 5.20 (1H, d, J=SHz), 5.77-6.34 (2H, m), 6.77-7.10 (1H, m), 7.63 (1H, s), 10.27 (1H, d, J=8Hz) (33) 7-[2-(2-Propynyloxyimino)-2-(2-aminothiazol-4 yl)acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3320, 2150, 1775, 1675 cm 1 (34) 7-[2-Methoxyimino-2-(2-aminothiazol.-4-yl) acetamido]-3-[1-(1-carboxyallyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3350, 1765, 1660, 1605 cm N.M.R. (DMSO-d6, #) : 3.55 (2H, m), 3.87 (3H, s), 4.37 (2H, m), 4.67-4.83 (5H, m), 6.75 (1H, s), 7.30 (2H, broad s), 9.60 (lH, d, J=8Hz) (35) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2 formamidothiazol-4-yl)acetamido]-3-[1-(3 carboxy-1-propenyl)-1H-tetrazol-5-yl]thiomethyl 3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1770, 1660 cm (36) 7-[2-(2-Cyclopenten-1-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(3-carboxy 1-propenyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1770, 1660 cm 1 (37) 7-[2-Phenoxyimino-2-(2-formamidothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). N.M.R. (DMSO-d6, d : 3.33-3.90 (6H, m), 4.10-4.67 (2H, m), 5.23 (1H, d, J=SMz), 5.80-6.33 (2H, m), 6.90-7.57 (6H, m), 7.73 (1H, s), 8.60 (1H, s), 9.03 (1M, d, J=8Hz), 12.86 (1H, broad s) (38) 7-[2-Phenoxyimino-2-(2-aminothiazol-4-yl)- acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). -1 I.R. (Nujol) : 3150, 1770, 1650, 1585 cm (39) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] -3-[1-[3-(4-methyl-1-piperazinyl)propyl]-lH-tetrazol-5-yll thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1760, 1660, 1600, 1530 cm 1. (40) 7-[2-methoxyimino-2- (2-aminothiazol-4-yl)acetamido] -3-[1-[3-(4-isopropyl-1-piperazinyl)propyl]-lH-tetrazol5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol) : 3350, 1765, 1660, 1600, 1530 cm (41) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-benzyl-1-piperanzinyl)propyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol): 3300, 1760, 1660, 1600 cm-1. (42) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamino]-3-[1-[3-(4-allyl-1-piperazinyl)propyl]-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. -1 I.R. (Nujol): 3350, 1770, 1670, 1600, 1530 cm (43) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-phenyl-1-piperazinyl)propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1665, 1600, 1530 cm 1 (44) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] -3-[1-[3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3350, 3250, 1775, 1670, 1610, 1540 cm 45)7- 45)7-[2-methoxyimino-2-(2-ami othiazol-4-yl)acetamido] 3-[1-[3-(4-ethoxycarbonyl-1-piperazinyl)propyl]-1H-tetrazol -5-yl]thiomelthyl-3-cephem-4-carboxylic acid (syn siomer), powder. I.R. (Nujol): 3300, 1770, 1670, 1600, 1530 cm-1. (46) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] -3-[1-[2-(4-methyl-1-piperazinyl)ethyl)-1H-tetrazol -5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1660, 1600, 1530 cm 1. (47) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido30 3-[1-(4-methyl-1-piperazinyl)carbonylmethyl-lH-tetrazol- 5-yll thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. -1 I.R. (Nujol): 3300, 1760, 1660, 1600, 1530 cm (48) 7&alpha;-methoxy-7ss-[D,L-2-carboxy-2-(4-hydroxyphenyl) acetamido]-3-[1-[3-(4-methyl-1-piperazinyl)propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid, powder. I.R. (Nujol): 3200, 1760, 1670, 1600, 1505 cm-1. (49) 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-[3-(4-methyl-1-piperazinyl)propyl] lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. -1 I.R. (Nujol): 3300, 1770, 1660, 1620, 1530 cm Examplel5 A solution of 7-[2-(2-cyclopenten-1-yl)oxyimino- 2-(2-formamidothiazol-4-yl)acetamido]-3-11-(2-carboxy- 2-hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxylic acid (syn isomer: in the 7-position) (1.1 g) and conc. hydrochloric acid (0.12 g) in methanol (10 m.1) and tetrahydrofuran (2 ml) was stirred at room temperature for 2.5 hours. After removal of organic solvents under reduced pressure, the residue was dissolved in an aqueous solution of sodium bicarbonate and the solution was adjusted to pH 7.5. The aqueous solution was washed with ethyl acetate (1; ml) and then adjusted to pM 3.0 with 10% hydrochloric acid to give precipitates which were filtered and washed with water to give 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(2-carboxy-2- hydroxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxwlic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3270, 3160, 1770, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 1.78-2.80 (4H, m), 3.70 (2, m), 4.38 (2H, q, J=14.0Hz), 4.97-5.51 (4H, m), 5.61-6.31 (3H, m), 6.73 (1H, s), 9.53 (1H, d, J=8;0Hz) Example 16 A mixture of 7-[2-methoxyimino-2-{2-(2,2,2- trifluoroaceamido)thiazol-4-yl}acetamido]-3-[1-(2 carboxy-2-metholminoethyl)-1H-tetrazol -5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position) (1.70 g) and sodium acetate trihydrate (3.26 g) in water (14 ml) was stirred overnight at room temperature. The solution was adjusted to pM 2.5 with 10% hydrochloric acid under ice-cooling and the resultant precipitates were filtered and washed with water to give 7-[2-methoxyimino-2-(2-aminothiazol- 4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3270, 1770, 1645 cm-1 N.M.R. (DMSO-d6, 6) : 9.60 (1H, d, J=8Hz), 7.13 (2H, broad s), 6.72 (1H, s), 5.77 (1H, dd, J=5 and 8Hz), 5.25 (2H, s), 5.09 (1H, d, J=8Hz), 4.32 (2H, ABq, J=14Hz), 3.95 (3H, s), 3.80 (3H, s), 3.65 (2H, broad s) Example 17 A mixture of 7-[2-(2-propynyloxyimino)-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3-[1-(2carboxy-2-methoxyiminoethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position)(2.0 g) and sodium acetate trihydrate (3.71 g) in water (15 ml) was stirred overnight at room temperature. The solution was adjusted to pM 2.5 with 10% hydrochloric acid under ice-cooling and the resultant precipitates were filtered and washed with water to give 7-[2-(2-propynyloxyimino)-2-(2-aminothiazol4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position).(1.12 g). I.R. (Nujol) : 3255, 1775, 1670, 1630 cm-1 N.M.R. (DMSO-d6, 6? : 9.57 (1H, d, J=8Hz), 7.17 (2H, broad s), 6.70 (1H, s), 5.73 (1H, dd, J=5 and 8Hz), 5.22 (2H, s), 5.07 (1H, d, J=5Hz), 4.64 (2H, m), 4.33 (2H, m), 3.95 (3H, s) , 3.63 (2H, m), 3.40 (1H, m) Example A mixture of 7-(2-(2-cyclopenten-l-yl)oxyiminc-2- (2-formamidothiazol-4-yl)acetamido]-3-[1-([3-(4-methyl-1piperazinyl)propyl]-1H-tetrazol-5-yl]thiomethyl-3cephem-4-carboxylic acid (syn isomer) (1.1 5), methanol (85 mQ) and conc-hydrochloric acid (0.6 m±) was stirred for 45 minutes at-room temperature. The reaction mixture was adjusted to pH 4.5 with a saturated aqueous solution of sodium bicarbonate and then concentrated. The concentrate was adjusted to pH 7 with a saturated aqueous solution of sodium bicarbonate and then subjected'to column chromatography (CA-cephadex, H-type: 110 mZ) , eluted with 1%, 2%, 4% and 5% aqueous solution of sodium chloride The fractions containing the object compound were collected and adjusted to pH 4.3 with a saturated aqueous solution of sodium bicarbonate. The resulting solution was subjected to column chromatography (Non-ion adsorption resin, Diaion HP20 prepared by Mitsubishi Chemical Industries: 50 ml), eluting with water and 20% isopropyl alcohol. The fractions containing the object compound were collected and concentrated under reduced pressure. The concentrate was lyophilized to give 7-[2-(2-cyclopenten-l-yl) oxyimino-2-(2-aminothiazol-4-yl)acetamido]-3-[3-(4methyl-1-piperazinyl)propyl]-1H-tetrazol-5-yl]thiomethyl3-cephem-4-carboxylic acid (syn isomer) (0.3 g). -l I.R. (Nujol): 3300, 1770, 1660, 1620, 1530 cm N.M.R. (DMSO-d6,#) : 1.9-3.2 (16H, m) , 2.63 (3H, s), 3.65 (2H, broad s), 4.28 (4H, broad s), 3.06 (lH, d, J = 5Hz), 5.2 (1H, broad s), 5.6-6.3 (3H, m), 6.54 (1H, s), 7.2 (2H, broad s), 10.13 (lH, d, J = 8Hz). Example 19 The following compounds were prepared according to the similar manners to those of Examples 15to 18. (1) 7-E2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamidol- 3-[1-(2-carboxy-2-hydroxyiminoethyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 169 to 173 C (dec.). I.R. (Nujol) : 3300, 3200, 1770, 1660, 1630 cm 1 N.M.R. (DMSO-d6, #) : 3.70 (2H, m), 3.86 (3H, s), 4.36 t2H, q, J=14.0Hz), 4.98-5.39 (3H, m), 5.80 (1H, dd, J=4.0 and 8.0Hz), 6.77 (1H, s), 9.60 (1H, d, J=8.0Hz) (2) 7-[2-Cyclopentyloxyimino-2-(2-aminothiazol-4 yl)acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 1710C (dec.). I.R. (Nujol) : 3250, 150, 1760, 1650 cm 1 N.M.R. (DMSO-d6, #) : 1.28-2.03 (8H, m), 3.67 (2H, m), 4.33 (2H, m), 4.86 (1H, m), 4.99-5.46 (3H, m), 5.73 (1H, dd, J=5 and 8.0Hz), 6.67 (1H, s), 9.49 (1H, d, J=8.0Hz) (3) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl) lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 160 to 1630C. I.R. (Nujol) : 3200, 1770, 1640 cm N.M.R. (DMSO-d6, 6) : 3.69 (2H, m), 4.41 (2H, q, J=14.0Hz), 5.03-5.48 (5H, m), 5.84 (1H, dd, J=5.0 and 8.0Hz), 6.81 (1H, s), 6.89-7.70 (4H, m), 9.72 (1H, d, J=8.0Hz) (4) 7-[2-Isopropoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[l-(2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 150 C I.R. (Nujol) : 3260, 1770, 1660 cm 1 N.M.R. (DMSO-d6) : 1.27 (6H, d, J=6Hz), 3.68 (2H, m), 3.98 (3H, s), 4.22-4.50 (3H, m), 5.16 (1H, d, J=5Hz), 5.27 (2H, s), 5.77 (1H, dd, J=5 and 8Hz), 6.73 (1H, s), 9.55 (1H, d, J=8Hz) (5) 7-[2-Allyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminothyl) lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 130 to 1400C (dec.). I.R. (Nujol) : 3250, 1770, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.73 (2H, m), 4.02 (3H, s), 4.40 (2H, m), 4.65 (2H, m), 5.31 (2H, 5), 5.60-5.07 (3H, m), 6.40-5.70 (2H, m) , 7.82 (1H, s), 9.69 (1H, d, J=8Hz) (6) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2-aminothiazol- 4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3260, 1770, 1650 cm 1 N.M.R. (DMSO-d6, #) : 1.80-2.43 (4H, m), 3.63 (2H, m), 3.97 (3H, s), 4.35 (2H, m) , 5.03-5.43 (4H, m), 5.60-6.20 (3H, m), 6.69 (1H, s), 9.50 (1H, d, J=8Hz) (7) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl) lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 168 to 172 C (dec.). I.R. (Nujol) : 3260, 1770, 1655 cm 1 N.M.R. (DMSO-d6, 6) : 3.67 (2H, m), 4.00 (3H, s), 4.37 (2H, ABq, J=14Hz), 5.17 (3H, m), 5.30 (2H, s), 5.84 (1H, dd, J=5 and 8Hz), 6.75 (1H, s), 7.37 -' (SH, m), 9.67 (1H, d, J=8Hz) (8) 7-[2-(4-Fluorobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)- lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 130 to 140 C (dec.). I.R. (Nujol) : 3270, 1770, 1625 cm N.M.R. (DMSO-d6, 6) : 3.63 (2H, m), 3.95 (3H, s), 4.33 (2H, ABq, J=14Hz), 5.11 (3H, m), 5.24 (2H, s), 5.79 (1H, dd, J=5 and 8Hz), 6.73 (1H, s), 6.97-7.60 (4H, m), 9.66 (1H, d, J=8Hz) (9) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-allyloxyiminoethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3270, 1770, 1660, 1630 cm 1 N.M.R. (DMSO-d6, 6) : 1.70-2.43 (4H, m), 3.67 (2H, m), 4.35 (2H, m), 4.73 (2H, m), 5.00-6.30 (1OH, m), 6.70 (1H, s), 9.48 (1H, d, J=8Hz) (10) 7-[2-Benzyloxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-allyloxyiminoethyl) lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 180 to 190 C (dec.). I.R. (Nujol) : 3290, 1775, 1705, 1655 cm-1 N.M.R. (DMSO-d6, 6) : 3.68 (2H, m), 4.40 (2H, m), 4.77 (2H, m), 5.03-5.50 (7H, m), 5.66-6.29 (2H, m), 6.77 (1H, s), 7.39 (5H, m), 9.71 (1H, d, J=8Hz) (11) 7-[2-(4-Flourobenzyl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyethyl) 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1765, 1630 cm 1 N.M.R. (DMSO-d6, 6) : 3.70 (2H, m), 4.20-4.70 (5H, m), 5.17 (3H, m), 5.82 (1H, dd, J=5 and 8Hz), 6.80 (1H, s), 7.03-7.61 (4H, m), 9.73 (1H, d, J=8Hz) (12) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-hydroxyethyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), mp 140 to 1500C (dec.). I.R. (Nujol) : 3320, 1770, 1635 cm 1 N.M.R. (DMSO-d6, 6) : 3.70 (2H, m), 3.86 (3H, s) , 4.13-4.78 (5H, m), 5.11 (1H, d, J=SHz), 5.76 (1H, dd, J=5 and 8Hz), 6.77 (1H, s), 9.62 (1H, d, J=8Hz) (13) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(2-carboxy-2-aminoethyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 1765, 1640 cm 1 N.M.R. (DMSO-d6, 6) : 3.72 (2H, m), 3.84 (3H, s), 4.00-4.91 (5H, m), 5.10 (1H, d, J'=SHz), 5.75 (1H, dd, J=5 and 8Hz), 6.74 (1H, s), 9.56 (1H, d, J=8Hz) (14) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3300, 1760, 1660 cm-1 (15) 7-[2-(2-Propynyloxyimino)-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(3-carboxy-1-propenyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3320, 2150, 1775, 1675 cm N.M.R. (DMSO-d6, 6) : 3.37 (2H, d, J=6Hz), 3.47 (1H, m), 3.70 (2H, m), 4.37 (2H, d, J=SHz), 4.70 (2H, d, J=2Hz), 5.10 (1H, d, J=6Hz), 5.77 (1H, d, J=8 and 6Hz), 6.30 (1H, ddd, J=2, 10 and 12Hz), 6.77 (1H, s), 7.00 (1H, m), 7.27 (2H, broad), 9.67 (1H, d, J=8Hz) (16) 7-[2-Methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[1-(1-carboxyallyl)-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3350, 1765, 1660, 1605 cm-1 (17) 7-[2-(2-Cyclopenten-l-yl)oxyimino-2-(2 aminothiazol-4-yl)acetamido]-3-[1-(3-carboxy 1-propenyl)-1H-tetrazol-5-yl]thiomethyl-3 cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3280, 1770, 1660 cm N.M.R. (DMSO-d6, 6) : 1.81-2.93 (4H, m), 3.40 (2H, q, J=16.OHz), 3.71 (2H, m), 4.39 (2H, q, J=14.0Hz), 5.06-5.54 (2H, m), 5.62-6.43 (4H, m), 6.74 (1H, s), 7.01 (1H, d), 9.53 (1H, d, J=8.OHz) (18) 7-[2-Phenoxyimino-2-(2-aminothiazol-4-yl acetamido]-3-[1-(3-carboxy-1-propenyl)-1H tetrazol-5-yllthiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R. (Nujol) : 3150, 1770, 1650, 1585 cm N.M.R. (DMSO-d6, 6) : 3.37 (2H, m) , 3.73 (2H, broad s), 4.37 (2H, q, J=12Hz), 5.17 (1H, d, J=SHz), 5.73-6.37 (2H, m), 6.87-7.33 (6H, m), 7.00 (1H, s), 9.80 (1H, d, J=8Hz) (19) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-[3-(4-methyl-1-piperanzinyl)propyl)]-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1760, 1660, 1600, 1530 cm-1 (20) 7-E2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-E 3-(4-isopropyl-1-piperazinyl)propyl]-lH-tetrazol-5- yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1660, 1600, 1530 cm 1 (21) Sodium 7- [2-methoxyimino-2- (2-aminothiazol-4-yl) acetamido]-3-[1-[3-(4-benzyl-1-piperanzyl)propyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol): 3300, 1760, 1660, 1600 cm 1. (22) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[3-(4-allyl-1-piperanzinyl)propyl)-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol) : 3350, 1770, 1670, 1600, 15.30 cm 1. (23) Sodium 7-[2-methoxyimino-2-(2-aminothiazol-4-yl) acetamido]-3-[ 3-(4-phenyl-1-piperazinyl)propyl3-1H- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylate (syn isomer), powder. I.R. (Nujol): 3300, 1765, 1665, 1600, 1530 cm-1 (24) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] -3-[1-[3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3350, 3250, 1775, 1670, 1610, 1540 cm-1. (24) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-tl-[3-(4-ethoxycarbonyl-l-piperazinyl)propyl3-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. I.R. (Nujol): 3300, 1770, 1670, 1600, 1530 cm ( 7- [2-methoxyimino-2- (2-aminothiazol-4-yl) acetamido] -3-[1-[2-(4-methyl-1-piperazinyl)ethyl]-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer), powder. T.R. (Nujol-): 3300, 1765, 1660, 1600, 1530 cm (27) 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3- 4-methyl-1-piperazinylicarbonylmethyl-lH-tetrazol-S- yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer) powder. I.R. (Nujol) : 3300, 1760, 1660, 1600, 1530 cm-1. Example 20 A solution of 7-[2-methoxyimino-2-{2-(2,2,2- trifluoroacetamido)thiazol-4-yl)acetamido]-3-[1-(2 carboxat-2-tert-butoxyzarboxamidoethyl)-lH-tetTazol-5- yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position) (1.1 g) in formic acid (11 ml) was stirred for 3 hours at 35 to 40 C. The reaction mixture was concentrated. The residue was pulverized in acetonitril (25 ml) and stirred vigorously. The precipitates ere collected by filtration and successively washed with acetonitril and diethyl ether to give 7-[2-methoxyimino-2-{2-(2,2,2-trifluoro- acetamido)thiazol-4-yl}acetamido]-3-[1-(2-carboxy-2aminoethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4carboxylic acid-formate (syn isomer: in the 7-position). I.R. (Nujol) : 3160, 1760, 1650 cm 1 N.M.R. (DMSO-d6, 6) : 3.73 (2H, m), 3.88 (3H, s), 4.00-4.83 (5H, m), 5.13 (1H, d, J=5Mz), 5.87 (1H, dd, J=5 and 8Hz), 7.33 (1H, s), 9.68 (1H, d, J=8Hz) Example 21 The following compound was prepared according to the similar manner to that of Example 20 7-L2-Methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-(2-carboxy-2-aminoethyl)-1H-tetrazol-5-yl]thiomethyl3-cephem-4-carboxylic acid (syn isomer: in the 7-position). I.R (Nujol) : 1765, 1640 cm

Claims: What we claim is 1. A new cephem compound of the formula: EMI126.1 wherein R1 is amino or a substituted amino group; R2 is carboxy or a protected carboxy group; R is a group of the formula: -Al-R3a [ in which A1 is hydroxy(lower)alkylene, amino (lower) alkylene, protected amino (lower) alkylene, lower alkenylene or hydroxyimino(lower) alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R3ais carboxy or a protected carboxy group) or a group of the formula: EMI126.2 [in which A2 is lower alkylene which may have an oxo group; and R3b is organic group which may have suitable substituent(s)3; and Q is hydrogen or lower alkoxy; and pharmaceutically acceptable salts thereof. 2. The compound of claim 1, wherein R1 is amino or an acylamino group; R2 is carboxy or an esterified carboxy group; R is a group of the formula: -Al-R3a [in which A1 is hydroxy(lower)alkylene, amino (lower) alkylene, acylamino (lower) - alkylene, hydroxyimino (lower) alkylene, lower alkoxyimino(lower)alkylene, lower alkenyloxyimino (lower) alkylene or lower alkenylene; and R3a is carboxy or an esterified carboxy group or a group of the formula: EMI127.1 [in which A2 is lower alkylene which may have an oxo group; and R b is carboxy, esterified carboxy, lower alkyl, lower alkenyl, hydroxy (lower)alkyl, ar(lower)alkyl or aryl; and Q is hydrogen or lower alkoxy. 3. The compound ofclaim 2, wherein R1 is amino; R2 is carboxy; R is a group of the formula: -Al-R3a [in which A1 is hydroxy (lower) alkylene, amino (lower) alkylene, lower alkoxycarbonylamino(lower)alkylene, hydroxyimino (lower) alkylene, lower alkoxyimino(lower)alkylene, lower alkenyloxyimino (lower) alkylene or lower alkenylene; and R a is carboxy] or a group of the formula: EMI127.2 [in which A2 is lower alkylene which may have an oxo group; and R3b is carboxy, lower alkoxycarbonyl, lower alkyl, lower alkenyl, hyderoxy (lower) alkyl, phenyl (lower) alkyl or phenyl]; and Q is hydrogen. 4. The compound of claim 3, which is selected from the group consisting of: 7-amino-3-[1-(2-carboxy-2-hydroxyethyl)-lH-tetrazol5-yl]thiomethyl-3-cephem-4-carboxylic acid, 7-amino-3-[1-(2-carboxy-2-tert-butoxycarboxamido ethyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4carboxylic acid, or its disodium salt, 7-amino-3- [1- (2-carboxy-2-hydroxyiminoethyl)-1H- tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid, 7-amino-3- [1- (2-carboxy-2-methoxyiminoethyl)-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid, 7-amino-3- [1- (2-carboxy-2-allyloxyiminoethyl)-lH tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid, 7-amino-3- [l-(3-carboxy-l-propenyl)-lH-tetrazol-5- yl]thiomethyl-3-cephem-4-carboxylic acid, 7-amino-3-[1-(2-carboxyallyl)-1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid, and 7-amino-3-[1-[3-(4-methyl-1-piperazinyl)propyl]-1Htetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid. 5. The compound of claim 2, wher RÚ is a group of the formula: (in which R5 amino or a protected amino; and EMI128.1 R6 is an organic residue which may have suitable substituent(s)) 6. The compound of claim 5, which is syn isomer. 7. The compound of claim 6, wherein EMI128.2 group is EMI128.3 8. The compound of claim 7, wherein R2 is carboxy; R5 is amino; and R6 is lower alkyl, lower alkenyl, lower alkynyl, cycloalkyl, cycloClower)alkenyl, aryl, ar(lower) alkyl which may have halogen. 9. The compound of claim 8, wherein R is a group of the formula: -Al-R3a [in which A1 is hydroxy(lower)alkylene, amino ower)alkylene, lower alkoxycarbonyl amino(lower)alkylene, hydroxyimino (lower)alkylene, lower alkoxyimino (lower)alkylene, lower alkenyloxyimino (lower)alkylene or lower alkenylene; and R3a is carboxy or an esterified carboxy group]; and Q is hydrogen. 10. The compound of claim 9, which is selected from the group consisting of: 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido 3-[1-(2-carboxy-2-hydroxyethyl)-lH-tetrazol-5-yl]- thiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-(2-carboxy-2-aminoethyl)-1H-tetrazol-5-yl] thiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2-aminothiazol- 4-yl)acetamido]-3-[1-(2-carboxy-2-hydroxyiminoethyl)- 1H-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), 7-L2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-(2-carboxy-2-methoxyiminoethyl)-lH-tetrazol-5- yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), 7-[2-isopropoxyimino-2-(2-aminothiazol-4-yl)acetamidol 3- [l"(Z-carboxy-2-methoxyiminoethyl)-lH-tetrazol-S-yl)- thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position) 7-[2-allyloxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-(2-carboxy-2-methoxyiminoethyl)-1H-tetrazol-5 yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), 7-|2-(2-propynyloxyimino)-2-(2-aminothiazol-4-yl)- acetamido]-3-[1-(2-carboxy-2-methoxyiminoethyl)-lH- tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamidol-3-[1-(2-carboxy-2-methoxyiminoethyl) lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer: in the 7-position), and 7-[2-(2-propynyloxyimino)-2-(2-aminothiazol-4-yl) acetamido) -3- [1-(3-carboxy-1-propenyl) -lH-tetrazol- 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer). 11. The compound of claim 8, wherein R is a group of the formula: EMI130.1 [in which A2 is lower alkylene which may have an oxo group; and R3b is carboxy, esterified carboxy, lower alkyl, lower alkenyl, hydroxy(lower)t alkyl, ar(lower)alkyl or aryl]; and Q is hydrogen. 12. The compound of claim 11, which is selected from the group consisting of: 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-[3-(4-methyl-1-piperazinyl)propyl]-lH-tetrazol- 5-yllthiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2"methoxyimino-2-(2-aminothiazol-4-yl)acetamido)- 3-[1-[3-(4-isopropyl-1-piperazinyl)propyl]-1H tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]- 3-[1-[3-(4-allyl-1-piperazinyl)propyl]-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), or its sodium salt, 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-[3-[4-(2-hydroxyethyl)-1-piperazinyl)propyl] lH-tetrazol-5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-[2-[4-methyl-1-piperazinyl)ethyl]-1H-tetrazol 5-yl]thiomethyl-3-cephem-4-carboxylic acid (syn isomer), 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido] 3-[1-(4-methyl-1-piperazinyl)carbonylmethyl]-1H tetrazol-5-yl) thiomethyl-3-cephem-4-carboxylic acid (syn isomer), and 7-[2-(2-cyclopenten-1-yl)oxyimino-2-(2-aminothiazol 4-yl)acetamido]-3-[1-[3-(4-methyl-1-piperazinyl)- propyl]-lH-tetrazol-5-yl]thiomethyl-3-cephem-4- carboxylic acid (syn isomer). 13. The compound of claim 2, wherein R1 is phenyl(lower)alkanoylamino which may have hvdroxv and/or carboxv. R is a group of the formula: EMI131.1 [in which A is lower alkylene: R is lower alkyl]; R2 is carboxy; and Q is lower alkoxy. 14. A process for preparing new cephem compounds of the formula: EMI131.2 wherein R1 is amino or a substituted amino group; R2 is carboxy or a protected carboxy group; R is a group of the formula: -Al-R3a [ in which A1 is hydroxy(lower)alkylene, amino(lower)alkylene,protected amino(lower)alkylene, lower alkenylene or hydroxyimino(lower) alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R3a is carboxy or a protected carboxy zrouDl or a group of the formula: EMI132.1 [in which A2 is lower alkylene which may have an oxo group; and R3b is organic group which may have suitable substituent(s)); and Q is hydrogen or lower alkoxy, or a pharmaceutically acceptable salt thereof, which comprises; 1) reacting a compound of the formula: EMI132.2 wherein R1, R2 and Q are each as defined above, and Y is a group which can be substituted by a group of the formula: (wherein R is as defined above), EMI132.3 or a salt thereof, with a compound of the formula: EMI133.1 wherein R is as defined above, or its reactive derivative at the mercapto group or a salt thereof, to give a compound of the formula: EMI133.2 wherein R1, R2, R and Q are each as defined above, or a salt thereof; or 2) reacting a compound of the formula: EMI133.3 wherein R2, R and Q are each as defined above, or its reactive derivatives at the amino group or a salt thereof, with an acylating agent, to give a compound of the formula: EMI134.1 wherein R2, R and Q are each as defined above; and Rla is acylamino, or a salt thereof; or 3), subjecting a compound of the formula: EMI134.2 wherein R2, R and Q are each as defined above; and Rla is acylamino having a protected amino group, or a salt thereof, to the elimination reaction of the amino protective group, to give a compound of the formula: EMI134.3 wherein R2, R and Q are each as defined above; and Rla" is acylamino having an amino group, or a salt thereof; or 4) subjecting a compound of the formula: EMI135.1 wherein R1, R2, R3a and Q are each as defined above; and A1' is a protected amino(lower)alkylene, or a salt thereof, to the elimination reaction of the amino protective group, to give a compound of the formula: EMI135.2 wherein R1, R2, R3a and Q are each as defined above; and A1,, is amino(lower)alkylene, or a salt thereof. 15. A 1-substituted-1H-tetrazole-5-thiol compound of the formula: EMI136.1 wherein R is a group of the formula: A1a-R3a [in which A1q is hydroxy (lower)alkylene, amino (lower) alkylene, protected amino (lower) alkylene, lower alkenylene, lower alkylene having an oxo group; or hydroxyimino (lower) alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R3a is carboxy or a protected carboxy group] or a group of the formula: EMI136.2 [in which A2 is lower alkylene which may have an oxo group; and R3b is organic group which may have suitable substituent(s)) and R10 is hydrogen or a mercapto-protective group, and a salt thereof. 16. A process for preparing a l-substituted-1H-tetrazole 5-thiol compound of the formula: EMI136.3 wherein R is a group of the formula: -Aal-R3a [in which As is hydroxy(lower)alkylene, amino (lower) alkylene, protected amino (lower) alkylene, lower alkenylene, lower alkylene having an oxo group; or or hydroxyimino (lower) alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R3a is carboxy or a protected carboxy group) or a group of the formula: EMI137.1 [in which A2 is lower alkylene which may have an oxo group; and R3b is organic group which may have suitable substituent(s)) and R10 is hydrogen or a mercapto-protective group, or a salt thereof, which comprises, 1) reacting a compound of the formula: EMI137.2 wherein X is lower alkylene, or its reactive derivative at the mercapto group, with a compound of the formula: R3a-COOH (VII) wherein R3a is as defined above, or its ester, to give a compound of the formula: EMI138.1 wherein X and R3a are each as defined above, or its reactive derivative at the mercapto group or a salt thereof; or 2) subjecting a compound of the formula: EMI138.2 wherein X is as defined above; and R3a is protected carboxy, or its reactive derivative at the mercapto group, to the elimination reaction of the carboxy protective group, to give a compound of the formula: EMI138.3 wherein X is as defined above; or its reactive derivative at the mercapto group or a salt thereof; or 3) reacting a compound of the formula: EMI139.1 wherein X and R3a are each as defined above; or its reactive derivative at the mercapto group or a salt thereof, with a compound of the formula: H2N-OR4 (VIII) wherein R4 is hydrogen or a lower aliphatic hydrocarbon group; or a salt thereof, to give a compound of the formula: EMI139.2 wherein X, R3a and R4 are each as defined above; or its reactive derivative at the mercapto group or a salt thereof; or 4) reducing a compound of the formula: EMI140.1 wherein X and R3a are each as defined above; or its reactive derivative at the mercapto group or a salt thereof, to give a compound of the formula: EMI140.2 wherein X and R3a are each as defined above; or its reactive derivative at the mercapto group or a salt thereof; or 5) reducing a compound of the formula: EMI140.3 wherein X, R3a and R4 are each as defined above; or its reactive derivative at the mercapto group or a salt thereof, to give a compound of the formula: EMI141.1 wherein X and R3a are each as defined above; or its reactive derivative at the mercapto group or a salt thereof; or 5) subjecting a compound of the formula: EMI141.2 wherein X and R3a are as defined above; or its reactive derivative at the mercapto group or a salt thereof, to the introduction reaction of the amino protective group, to give a compound of the formula: EMI141.3 wherein X and R3a are each as defined above; and Z is a protected amino group; or its reactive derivative at the mercapto group or a salt thereof; or 7) subjecting a compound of the formula: EMI142.1 wherein B is lower alkenylene, or its reactive derivative at the mercapto group, to the reaction to introduce a carboxy group, to give a compound of the formula: EMI142.2 wherein R3a is as defined above; and B is lower alkenylene or its reactive derivative at the mercapto group or a salt thereof; or 8) reacting a compound of the formula: EMI142.3 wherein A2 and R3b are each as defined above, and R9 is lower alkyl or a salt thereof, with a hydrazoic acid salt (XII), to give a compound of the formula: EMI143.1 wherein A2 and R3b are each as defined above, or a salt thereof; or 9) reacting a compound of the formula: EMI143.2 wherein A is as defined above, and R is a mercapto protective group, or its reactive derivative at the hydroxy group, with a compound of the formula: EMI143.3 wherein R3b is as defined above, or a salt thereof, to give a compound of the formula: EMI143.4 wherein A2, R3b and R10a are each as defined above, or a salt thereof; or 10) subjecting the compound of the formula: EMI144.1 wherein A2, R3b and RlOa are each as defined above, o a salt thereof, to the elimination reaction of the mercapto-protective group, to give a compound of the formula: EMI144.2 wherein A2 and R3b are each as defined above, or a salt thereof; or 11) reacting a compound of the formula: EMI144.3 wherein A2, is lower alkylene, or its reactive derivative at the carboxy group or a salt thereof, with the compound of the formula: EMI145.1 wherein R3b is as defined above, or its reactive derivative at the amino group or a salt thereof, to give a compound of the formula: EMI145.2 wherein A2 and R3b are each as defined above, or a salt thereof; or 17. An aminothiazolacetic acid compound of the formula: EMI145.3 wherein R5 is amino or a protected amino group; and R6a is aryl. or a salt thereof. 18. A process for preparing an aminothiazolylacetic acid compound of the formula: EMI146.1 wherein R5 is amino or a protected amino group; and R6a is aryl, or a salt thereof, which comprises reacting a compound of the formula: EMI146.2 wherein R5 is as defined above, or a salt thereof, with a compound of the formula: H2N-O-R6a (X) wherein R6a is as defined above, or a salt thereof. 19. A new cephem compound of the formula: EMI146.3 wherein R a is phenylacetamido having hydroxy and/or carboxy; R2 is carboxy or a protected carboxy group; Y is a group which can be substituted by a group of the formula: [wherein R is a group of the formula: -Al-R3a EMI147.1 [in which A1 is hydroxy(lower)alkylene, amino (lower)alkylene protected amino (lower)alkylene, lower alkenylene or hydroxyimino (lower) alkylene wherein the hydrogen atom of the hydroxyimino group may be replaced with a lower aliphatic hydrocarbon group; and R3a is carboxy or a protected carboxy group] or a group of the formula: EMI147.2 [in which A2 is lower alkylene which may have an oxo group; and R3b is organic group which may have suitable substituent(s)]]; and Q is lower alkoxy; 20. A pharmaceutical antibacterial composition comprising a compound of claim 1 in association with a pharmaceuti cally acceptable, substantially non-toxic carrier or excipient.

Inventors: INOUE, YOSHIKAZU, KATO, MASAYUKI, MATSUO, MASAAKI, MURATA, MASAYOSHI, TAKAYA, TAKAO, TSUJI, KIYOSHI, UEDA, IKUO, YASUDA, NOBUYOSHI
Applicants: FUJISAWA PHARMACEUTICAL CO., LTD.
Application year: 1980
Main classification: C07D501
IPC codes: C07D501, C07D257, C07D277, C07D295, C07D209