STARTING COMPOUNDS FOR 7-ACYLAMINO-3-VINYLCEPHALOSPORANIC ACID DERIVATIVES AND PROCESSES FOR THEIR PREPARATION

Abstract: Carboxylic acid derivative of the formula R¹-A¹³-R e (5 ○-b) in which R¹ is amino-substituted-heterocyclic group which may have halogen, protected amino-substituted-heterocyclic group which may have halogen, or a group of the formula: wherein R³ is lower alkyl, A¹³ is lower alkylene having a group of the formula: = N-ORh⁴ R e is carboxy or a protected carboxy group, or a salt thereof and process for the preparation thereof.

Description: The present invention relates to starting compounds for 7-acylamino-3-vinylcephalosporanic acid deriv atives and pharmaceutically acceptable salts thereof, which have antimicrobial activity, and to processes for the preparation thereof. This application is a divisional application from EP-A2-123024 which by itself is a divisional application from EP-A2-30630 disclosing 7-acylamino-3-vinylcephalosporanic acid derivatives, processes for their preparation and pharmaceutical compositions containing them. EP-A-8442 shows the compound of the formula: wherein R³ is amino or a protected amino group, and R⁴ is lower alkyl having halogen atom(s); or lower unsaturated aliphatic group such as lower alkenyl or lower alkynyl. GB-A-2033377 shows the compounds of the formula: wherein R¹ is-amino or protected amino, R² is lower alkyl, amino(lower)alkyl, protected amino(lower)alkyl, hydroxy(lower)alkyl, protected hydroxy(lower)alkyl, lower alkylthio(lower)alkyl, carboxy(lower)alkyl, esterified carboxy(lower)alkyl, (C₃ to C₈)cycloalkyl, lower alkenyl or lower alkynyl. DE-A-2810922 shows the compounds of the formula: wherein R² is lower alkyl having 2 to 8 carbon atoms, cycloalkyl, lower alkenyl, having 2 to 6 carbon atoms, lower alkynyl having 2 to 6 carbon atoms, halo(lower)alkyl, carboxy(lower)alkyl or lower alkoxycarbonyl(lower)alkyl, R⁶ is amino, substituted or unsubstituted(lower)alkanamido or tritylamino, and R⁸ is hydrogen or lower alkyl, provided that when R² is ethyl, isopropyl or allyl, then R⁶ is amino or unsubstituted(lower)alkanamido, and R⁸ is hydrogen. DE-A-2812625 shows the compounds of the formula: wherein R₂ is selected from the group consisting of chloroacetyl and groups easily removable by acid hydrolysis or hydrogenolysis, R₃ is selected from the group consisting of -CN, -CONH₂ and -COOR₁'' and R₁'' is alkyl of 1 to 3 carbon atoms or an ester group easily removable by acid hydrolysis or hydrogenolysis, and R' and R'' are individually selected from the group consisting of hydrogen and alkyl of 1 to 3 carbon atoms. The object compounds of the present invention, which is useful for the starting compounds for 7-acylamino-3-vinylcephalosporanic acid derivates, are novel and can be represented by the following general formula: in which R¹ is aminothiazolyl which may have halogen, aminothiadiazolyl, protected aminothiazolyl which may have halogen or protected aminothiadiazolyl, R 4 / h is C₂-C₇alkenyl substituted by carboxy or a protected carboxy group, C₁-C₇alkyl substituted by a protected amino and a protected carboxy groups, or C₁-C₇alkyl substituted by a protected amino- and a protected carboxy-substituted-(C₁-C₇)alkoxycarbonyl, and R e is carboxy or a protected carboxy group. In the object compounds (5 ○-b) and the corresponding starting compound (5 ○-a) in the Process mentioned below, it is to be understood that there may be one or more stereoisomeric pair(s) such as optical and geometrical isomers due to asymmetric carbon atom and double bond in those molecules and such isomers are also included within the scope of the present invention. With regard to geometrical isomers in the object compounds, it is to be noted that, they include syn isomer, anti isomer and a mixture thereof, and the syn isomer means one geometrical isomer having the partial structure represented by the following formula: wherein R¹ and R 4 / h are each as defined above, and the anti isomer means the other geometrical isomer having the partial structure represented by the following formula: wherein R¹ and R 4 / h are each as defined above. Suitable pharmaceutically acceptable salts of the object compounds (5 ○-b) are conventional non-toxic salts and may include a salt with a base or an acid addition salt such as a salt with an inorganic base, for example, an alkali metal salt (e.g. sodium salt, potassium salt), an alkaline earth metal salt (e.g. calcium salt, magnesium salt), an ammonium salt; a salt with an organic base, for example, an organic amine salt (e.g. triethylamine salt, pyridine salt, picoline salt, ethanolamine salt, triethanolamine salt, dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt); an inorganic acid addition salt (e.g. hydrochloride, hydrobromide, sulfate, phosphate); an organic carboxylic or sulfonic acid addition salt (e.g. formate, acetate, trifluoroacetate, maleate, tartrate, methanesulfonate, benzenesulfonate, p-toluenesulfonate); a salt with a basic or acidic amino acid (e.g. arginine, aspartic acid, glutamic acid); or an intermolecular or intramolecular quaternary salt. According to the present invention. the object compound (5 ○-b) and the pharmaceutically acceptable salts thereof can be prepared by the process as illustrated by the following reaction scheme. Process in which R¹, R 4 / h and R e are each as defined above In the above and subsequent description of the present specification, suitable examples and illustration of the various definitions to be included within the scope thereof are explained in detail as follows. Suitable "C₁-C₇ alkyl" group may include straight or branched one such as methyl, ethyl, propyl, ispropyl, butyl, isobutyl, pentyl, isopentyl, neopentyl and hexyl, in which the preferred one is C₁-C₄ alkyl. Suitable "C₂-C₇ alkenyl" group may include straight or branched one such as vinyl, 1-propenyl, allyl, 1-(or 2- or 3-)butenyl, 1-(or 2- or 3- or 4-)pentenyl, 1-(or 2- or 3- or 4- or 5-)hexenyl and 2-methyl-2-propenyl, in which the preferred one is C₂-C₅ alkenyl. Suitable "protected amino" group may include an amino group substituted by a conventional amino-protective group which is used in penicillin and cephalosporin compounds. for example, acyl as mentioned below, aryl(C₁-C₇)alkyl such as mono-(or di or tri)phenyl(C₁-C₇)-alkyl (e.g. benzyl. benzhydryl, trityl), C₁-C₇ alkoxycarbonyl(C₁-C₇) alkylidene or its enamine tautomer (e.g. 1-methoxycarbonyl-1-propen-2-yl) or di (C₁-C₇)-alkylaminomethylene (e.g. dimethylaminomethylene). Suitable "acyl" may include an aliphatic acyl, an aromatic acyl, a heterocyclic acyl and an aliphatic acyl substituted with aromatic or heterocyclic group(s). The aliphatic acyl may include saturated or unsaturated, acyclic or cyclic ones, such as C₁-C₇ alkanoyl (e.g. formyl, acetyl, propionyl, butyryl, isobutyryl. valeryl, isovaleryl, pivaloyl or hexanoyl), C₁-C₇ alkanesulfonyl (e.g. mesyl, ethanesulfonyl, propanesulfonyl), C₁-C₇ alkoxycarbonyl (e.g. methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, tert-butoxycarbonyl), C₂-C₇ alkenoyl (e.g. acryloyl, methacryloyl, crotonoyl), ' (C₃-C₇)-cycloalkanecarbonyl (e.g. cyclohexanecarbonyl, and amidino. The aromatic acyl may include aroyl (e.g. benzoyl, toluoyl, xyloyl) and arenesulfonyl (e.g. benzenesulfonyl, tosyl). The heterocyclic acyl may include heterocyclecarbonyl (e.g. furoyl, thenoyl, nicotinoyl, isonicotinoyl, thiazolylcarbonyl, thiadiazolylcarbonyl, tetrazolylcarbonyl). The aliphatic acyl substituted with aromatic group(s) may include ar (C₁-C₇)alkanoyl such as phenyl(C₁-C₇)alkanoyl (e.g. phenylacetyl, phenylpropionyl, phenylhexanoyl), ar (C₁-C₇) alkoxycarbonyl such as phenyl(C₁-C₇) alkoxycarbonyl (e.g. benzyloxycarbonyl, phenethyloxycarbonyl) and phenoxy(C₁-C₇) alkanoyl (e.g. phenoxyacetyl, phenoxypropionyl). The aliphatic acyl substituted with heterocyclic group(s) may include thienylacetyl, imidazolylacetyl, furylacetyl, tetrazolylacetyl, thiazolylacetyl, thiadiazolylacetyl, thienylpropionyl and thiadiazolylpropionyl. These acyl groups may be further substituted with one or more suitable substituents such as C₁-C₇ alkyl (e.g. methyl, ethyl, propyl, isopropyl, butyl, pentyl, hexyl), halogen (e. g. chlorine, bromine, iodine, fluorine), C₁-C₇ alkoxy (e.g. methoxy, ethoxy, propoxy, isopropoxy, butoxy, pentyloxy, hexyloxy), C₁-C₇ alkylthio (e.g. methylthio, ethylthio, propylthio, isopropylthio, butylthio, pentylthio and hexylthio), nitro, and preferable acyl having such substituent(s) may be mono (or di or tri)halo(C₁-C₇)-alkanoyl (e.g. chloroacetyl, bromoacetyl, dichloroacetyl, trifluoroacetyl), mono (or di or tri)halo(C₁-C₇)-alkoxycarbonyl (e.g. chloromethoxycarbonyl, dichloromethoxycarbonyl, 2,2,2-tri-chloroethoxycarbonyl), nitro (or halo or C₁-C₇ alkoxy)phenyl (C₁-C₇) alkoxycarbonyl (e.g. nitrobenzyloxycarbonyl, chlorobenzyloxycarbonyl, methoxybenzyloxycarbonyl). Suitable "protected carboxy" group may include an esterified carboxy group which is conventionally used in penicillin or cephalosporin compounds at their 3rd or 4th position thereof. Suitable "ester moiety" in "esterified carboxy group" may include C₁-C₇ alkyl ester (e.g. methyl ester, ethyl ester, propyl ester, isopropyl ester, butyl ester, isobutyl ester, t-butyl ester, pentyl ester, tertpentyl ester, hexyl ester), C₂-C₇ alkenyl ester (e.g. vinyl ester, allyl ester), C₂-C₇ alkynyl ester (e.g. ethynyl ester, propynyl ester), C₁-C₇ alkoxy (C₁-C₇) alkyl ester (e.g. methoxymethyl ester, ethoxymethyl ester, isopropoxymethyl ester, 1-methoxyethyl ester, 1-ethoxyethyl ester), C₁-C₇ alkylthio (C₁-C₇) alkyl ester (e.g. methylthiomethyl ester, ethylthiomethyl ester, ethylthioethyl ester isopropylthiomethyl ester), amino- and carboxy-substituted- C₁-C₇ alkyl ester (e.g. 2-amino-2-carboxyethyl ester, 3-amino-3-carboxypropyl ester), protected amino- and protected carboxy-substituted- C₁-C₇ alkyl ester such as C₁-C₇ alkoxycarbonylamino- and mono-(or di to tri)phenyl (C₁-C₇) alkoxycarbonyl-substituted- C₁-C₇ alkyl ester (e.g. 2-tert-butoxycarbonylamino-2-benzhydryloxycarbonylethyl ester, 3-tert-butoxycarbonylamino-3-benzhydryloxycarbonylpropyl ester), mono(or di or tri)halo (C₁-C₇) alkyl ester (e.g. 2-iodoethyl ester, 2,2,2-trichloroethyl ester), C₁-C₇ alkanoyloxy-(C₁-C₇) alkyl ester (e. g. acetoxymethyl ester, propionyloxymethyl ester, butyryloxymethyl ester, isobutyryloxymethyl ester, valeryloxymethyl ester, pivaloyloxymethyl ester, hexanoyloxymethyl ester, 2-acetoxyethyl ester, 2-propionyloxyethyl ester, 1-acetoxypropyl ester), C₁-C₇ alkanesulfonyl (C₁-C₇) alkyl ester (e.g. mesylmethyl ester, 2-mesylethyl ester), aryl (C₁-C₇)-alkyl ester which may have one or more substituent(s) such as mono(or di or tri)phenyl (C₁-C₇) alkyl ester which may have one or more suitable substituent(s) (e.g. benzyl ester, 4-methoxybenzyl ester, 4-nitrobenzyl ester, phenethyl ester, benzhydryl ester, trityl ester, bis(methoxyphenyl)methyl ester, 3,4-dimethoxybenzyl ester, 4-hydroxy-3,5-di-t-butylbenzyl ester), aryl ester which may have one or more suitable substituents (e.g. phenyl ester, tolyl ester, t-butylphenyl ester, xylyl ester, mesityl ester, cumenyl ester, salicyl ester) and heterocyclic ester (e.g. phthalidyl ester). Suitable "halogen" may include chloro, bromo and iodo. Particularly, the preferred embodiments of the terms "R¹" and "R 4 / h" of the object compounds (5 ○-b) are as follows. The formula: is preferably a group of the formula: in which R¹ is preferably 2-aminothiazol-4-yl, 2-aminothiazol-5-yl, 2-amino-5-halothiazol-4-yl, 4-aminothiazol-2-yl, 5-amino-1,2,4-thiadiazol-3-yl, acylaminothiazolyl which may have halogen [more preferably 2-C₁-C₇alkanamidothiazol-4-yl (e.g. 2-formamidothiazol-4-yl), 4- C₁-C₇alkoxycarbonylaminothiazol-2-yl (e.g. 4-tert-butoxycarbonylaminothiazol-2-yl)], di(C₁-C₇)alkylaminomethyleneaminothiadiazolyl [more preferably 5-di(C₁-C₇)alkylaminomethyleneamino-1,2,4-thiadiazol-3-yl (e.g. 5-dimethylaminomethyleneamino-1,2,4-thiadiazol-3-yl)]. R 4 / h is carboxy(C₁-C₇)alkenyl (e.g. 3-carboxyallyl), esterified carboxy(C₁-C₇)alkenyl [more preferably C₁-C₇ alkoxycarbonyl(C₁-C₇)-alkenyl (e.g. 3-tert-butoxycarbonylallyl)], C₁-C₇ alkoxycarbonylamino- and mono- or di- or triphenyl(C₁-C₇)-alkoxycarbonyl-substituted-C₁-C₇ alkoxycarbonyl(C₁-C₇)alkyl (e.g. 2-tert-butoxycarbonylamino-2-benzhydryloxycarbonylethoxycarbonylmethyl), acylamino- and esterified carboxy-substituted-C₁-C₇ alkyl [more preferably C₁-C₇ alkoxycarbonylamino- and mono- or di- or triphenyl(C₁-C₇)-alkoxycarbonyl-substituted-C₁-C₇ alkyl (e.g. 3-tert-butoxycarbonylamino-3-benzhydryloxycarbonylpropyl)]. Process for the preparation of the object compound is explained in the following. Process : The compound (5 ○-b) or a salt thereof can be prepared by reacting the compound (5 ○-a) or a salt thereof with the compound (XV) or a salt thereof. Suitable salts of the compounds (5 ○-a) and (XV) may include the same ones as exemplified for the compound (5 ○-b ). This reaction can be carried out in the presence of an organic or inorganic base such as alkali metal (e.g. lithium, sodium, potassium), alkaline earth metal (e.g. calcium), alkali metal hydride (e.g. sodium hydride), alkaline earth metal hydride (e.g. calcium hydride), alkali metal hydroxide (e.g. sodium hydroxide, potassium hydroxide), alkali metal carbonate (e.g. sodium carbonate, potassium carbonated), alkali metal bicarbonate (e.g. sodium bicarbonate, potassium bicarbonate), alkali metal alkoxide (e.g. sodium methoxide, sodium ethoxide, potassium tert-butoxide), alkali metal alkanoic acid (e.g. sodium acetate), trialkylamine (e.g. triethylamine), pyridine compound (e.g. pyridine, lutidine, picoline) and quinoline. This reaction is usually carried out in a conventional solvent which does not adversely influence the reaction such as water, methanol, ethanol, propanol, dioxane, tetrahydrofuran, or a mixture thereof. The reaction temperature is not critical and the reaction is usually carried out under cooling to warming. The object compounds (5 ○-b) obtained according to the Process as explained above can be isolated and purified in a conventional manner, for example by extraction, precipitation, fractional crystallization, recrystallization or chromatography. It is to be noted that, in the aforementioned reaction or the post-treatment of the reaction mixture, in case that the starting or object compounds possess an optical and/or geometrical isomer(s), it may occasionally be transformed into the other optical and/or geometrical isomer(s), and such cases are also included within the scope of the present invention. In case that the object compound (5 ○-b) have a free carboxy group or free amino group, it may be transformed into its pharmaceutically acceptable salts by a conventional method. The compounds and the pharmaceutically acceptable salts thereof derived from the object compound of the present invention are novel and exhibit high antimicrobial activity, inhibiting the growth of a wide variety of pathogenic microorganisms including Gram-positive and Gram-negative microorganisms and are useful as antimicrobial agents, especially for oral administration as shown in the following data. Now in order to show the utility of the object compounds (5 ○-b), the test data on the antimicrobial activity of some representative compounds derived from the object compound of this invention are shown in the following. (1) Test: in vitro Antimicrobial Activities. Test Compounds No. 1 7- [2-(2-Aminothiazol-4-yl)-2-(L-2-amino-2-carboxyethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid (syn isomer) (hereinafter referred to as compound A) No. 2 7-[2-(2-Aminothiazol-4-yl)-2-(trans-3-carboxyallyloxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid (syn isomer)(hereinafter referred to as Compound B). Test Method In vitro Antimicrobial activity was determined by the two-fold agar-plate dilution method as described below. One loopful of an overnight culture of each test strain in Tripticase-soy broth (approximately 10⁸ viable cells per ml) was streaked on heart infusion agar (HI-agar) containing graded concentrations of antimicrobial agents, and the minimal inhibitory concentration (MIC) was expressed in term of µg/ml after incubation at 37°C for 20 hours. The following examples are given for the purpose of illustrating the present invention. Preparation of the starting compounds Preparation 1 To a suspension of L-serine (50 g) in water (500 ml) and dioxane (500 ml) were added triethylamine (140 ml) and 2-tert-butoxycarbonyloxyimino-2-phenylacetonitrile (138 g), and the mixture was stirred at ambient temperature for 24 hours. After removal of the dioxane, the remained aqueous solution was adjusted to pH 8.0 with an aqueous sodium bicarbonate and then washed four times with ethyl acetate (200 ml). The aqueous solution was adjusted to pH 2.0 with conc. hydrochloric acid and then extracted twice with ethyl acetate (300 ml). The combined ethyl acetate solution was washed with an aqueous sodium chloride, dried over anhydrous magnesium sulfate and then concentrated under reduced pressure. To the concentrate was added dropwise a solution of diazodiphenylmethane in ethyl acetate till the starting compound was disappeared on thin layer chromatography. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain N-tert-butoxycarbonyl-L-serine benzhydryl ester (109 g). I.R. (Nujol) : 3250, 1746, 1677 cm⁻¹ N.M.R. δppm (DMSO-d₆) : 1.37 (9H, s), 3.73 (2H, t, J=12.0Hz), 4.20 (1H, m), 4.93 (1H, t, J=12Hz), 6.82 (1H, s), 7.40 (10H, broad s) Preparation 2 To a solution of bromoacetyl bromide (20 g) in tetrahydrofuran (200 ml) were added N-tert-butoxycarbonyl-L-serine benzhydryl ester (10.84 g) and N,N-dimethylaniline (6.δ ml), and the mixture was stirred at 20 to 23°C for 80 minutes. After adjusting to pH 5.0 with 10% aqueous sodium hydroxide and 5% aqueous sodium bicarbonate, the tetrahydrofuran was removed by evaporation. The residue was dissolved in a mixture of ethyl acetate (200 ml) and water (100 ml), and then washed with 5% hydrochloric acid and an aqueous sodium chloride, followed by drying over anhydrous magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-bromoacetate (21.3 g), mp 92-94°C. IR (Nujol) : 3350, 1735, 1727, 1704, 1160 cm⁻¹ NMR δppm (DMSO-d₆) : 1.40 (9H, s), 4.03 (2H, s), 4.67 (3H, m), 6.85 (1H, s), 7.37 (10H, s) Preparation 3 A mixture of L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-bromoacetate (20 g), N-hydroxyphthalimide (6.7 g), triethylamine (8.5 ml) and N,N-dimethylformamide (80 ml) was stirred at 10 to 15°C for half an hour. The reaction mixture was poured into 5% aqueous sodium chloride (1.5 ℓ), and the precipitated material was collected by filtration and then washed with water, followed by dissolving in ethyl acetate (500 ml). The solution was washed twice with an aqueous sodium chloride and dried over anhydrous magnesium sulfate, followed by evaporation to give a residue, which was pulverized with diisopropyl ether to obtain L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-phthalimidooxyacetate (24.5 g), mp 45-50°C. IR (Nujol) : 3420, 1740, 1720 (shoulder) cm⁻¹ NMR δppm (DMSO-d₆) : 1.38 (9H, s), 4.55 (3H, broad s), 4.89 (2H, broad s), 6.93 (1H, s), 7.47 (10H,broad s), 8.00 (4H, s) Preparation 4 To a solution of L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-phthalimidooxyacetate (20 g) in methylene chloride (100 ml) was added a solution of hydrazine monohydrate (3.5 g) in methanol under icecooling, and the mixture was stirred below 15°C for an hour. The precipitated material was collected by filtration and washed with methylene chloride. The washings and the above obtained methylene chloride solution were combined, adjusted to pH 7.0 with 5% hydrochloric acid, and washed with an aqueous sodium chloride, followed by drying over anhydrous magnesium sulfate. Removal of the solvent gave a residue, which was chromatographed on silica gel (200 ml) eluting with a mixed solvent of benzene and ethyl acetate, and fractions containing a desired compound were collected. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-aminooxyacetate (10.5 g), mp 90-92°C. IR (Nujol) : 3400, 1745, 1720 cm⁻¹ NMR δppm (DMSO-d₆) : 1.38 (9H, s), 4.10 (2H, s), 4.45 (3H, broad s), 6.30 (2H, s), 6.84 (1H, s), 7.37 (10H, s) Preparation 5 A mixture of DL-homoserine (50 g), triethylamine (140 ml), 2-tert-butoxycarbonyloxyimino-2-phenylacetonitrile (103.3 g), water (500 ml) and dioxane (500 ml) was stirred at ambient temperature for 24 hours. After removal of the dioxane, the remained aqueous solution was adjusted to pH δ.5-9.0 with 10% aqueous sodium hydroxide and then washed with ethyl acetate (500 ml x 5). The resultant aqueous solution was adjusted to pH 2.0 with conc. hydrochloric acid and then extracted with ethyl acetate, followed by washing with an aqueous sodium chloride and drying over anhydrous magnesium sulfate. To this solution was added dropwise a solution of diazodiphenylmethane in ethyl acetate till the starting compound was disappeared on thin layer chromatography. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain N-tert-butoxycarbonyl-DL-homoserine benzhydryl ester (117.0 g), mp 125-129°C. IR (Nujol) : 3500, 3320, 1735, 1687 cm⁻¹ NMR δppm (CDCl₃) : 1.43 (9H, s), 1.8-2.5 (2H, m), 3.6 (2H, m), 4.58 (1H, m), 5.5 (1H, d, J=8Hz), 6.92 (1H, s), 7.3 (10H, s) Preparation 6 To a solution of N-hydroxyphthalimide (4.3 g), N-tert-butoxycarbonyl-DL-homoserine benzhydryl ester (10 g) and triphenylphosphine in tetrahydrofuran (100 ml) was added dropwise a diethyl diazenedicarboxylate (4.6 g) at ambient temperature with stirring, and the stirring was continued at 32 to 35°C for 3 hours. Removal of the solvent gave a residue, which was chromatographed on silica gel eluting with a mixed solvent of benzene and acetone. Fractions containing a desired compound were collected and then evaporated to obtain benzhydryl DL-2-tert-butoxycarbonylamino-4-phthalimidooxybutyrate (10 g), mp 162-163°C. IR (Nujol) : 3360, 1740, 1712, 1681 cm⁻¹ NMR δppm (CDCl₃) : 1.45 (9H, s), 2.37 (2H, q, J=6Hz), 4.26 (2H, t, J=6Hz), 4.58 (1H, m), 5.73 (1H, d, J=δHz), 6.90 (1H, s), 7.3 (10H, s), 7.77 (4H, s) Preparation 7 A solution of benzhydryl DL-2-tert-butoxycarbonylamino-4-phthalimidooxybutyrate (7.0 g) in methylene chloride (100 ml) was added dropwise to a solution of hydrazine monohydrate (2.0 g) in methanol (6 ml) at ambient temperature, and the mixture was stirred for half an hour. The precipitated material was collected by filtration and then washed with methylene chloride (30 ml). After the filtrate and washings were combined, thereto was added water, followed by adjusting to pH 7.0 with conc.hydrochloric acid. The separated methylene chloride solution was washed with water and an aqueous sodium chloride, followed by drying over anhydrous magnesium sulfate. Removal of the solvent gave a residue which was pulverized with diisopropyl ether to obtain benzhydryl DL-2-tert-butoxycarbonylamino-4-aminooxybutyrate (5.0 6), mp 92-93°C. IR (Nujol) : 3340, 3305, 1730, 1695 cm⁻¹ NMR σppm (DMSO-d₆) : 1.38 (9H, s), 1.95 (2H, m), 3.58 (2H, t, J=6Hz), 4.18 (1H, m), 5.92 (2H, s), 6.82 (1H, s), 7.37 (10H, s) Preparation 8 To a solution of N-hydroxyphthalimide (70.08 g) in acetonitrile (300 ml) were added triethylamine ( 48 g) and tert-butyl 4-bromocrotonate (96.0 g) with stirring, and the mixture was refluxed under heating for 1.5 hours. The reaction mixture was poured into water (600 ml), followed by extraction with ethyl acetate. The extract was washed with a saturated aqueous sodium chloride, dried over magnesium sulfate and then evaporated to dryness under reduced pressure to give a residue, which was pulverized with n-hexane. The resultant substance was subjected to column chromatography on silica gel eluting with a mixed solvent of n-hexane, ethyl acetate and diisopropyl ether (5:0.5:4.5 by volume), and the fractions containing a desired compound were collected. Removal of the solvent gave a residue, which was pulverized with n-hexane and collected by filtration to obtain tert-butyl 4-phthalimidooxycrotonate (41.7 g). NMR δppm (DMSO-d₆) : 1.45 (9H, s), 4.90 (2H, m), 6.09 (1H, m), 6.66-7.19 (1H, m), 7.86 (4H, s) preparation 9 To a solution of tert-butyl 4-phthalimidooxycrotonate (20.0 g) in methylene chloride (140 ml) was added a solution of hydrazine monohydrate (5.0 g) in methanol (10 ml) with stirring, and the stirring was continued at ambient temperature for 15 minutes. The insoluble substance was collected by filtration and washed with methylene chloride. The washings and the filtrate were combined and then extracted three times with 5% hydrochloric acid. After the combined extracts were washed with diethyl ether, thereto was added methylene chloride, followed by adjusting to pH 7.5 with 28% aqueous ammonium hydroxide. The separated methylene chloride solution was washed with a saturated aqueous sodium chloride and then dried over magnesium sulfate. Removal of the solvent gave an oil of tert-butyl 4-aminooxycrotonate (11.03 g). IR (Film) : 3340, 3250, 2980, 2940, 1720, 1660 cm⁻¹ NMR δppm (DMSO-d₆) : 1.43 (9H, s), 4.18 (2H, m), 5.85 (1H, m), 6.14 (2H, broad s), 6.52-7.06 (1H, m) Preparation of the object compounds Example 1 To tert-butyl 4-aminooxycrotonate (10.0 g) were added ethanol (150 ml) and water (150 ml), followed by gradually adding (2-formamidothiazol-4-yl)glyoxylic acid (11.0 g) with stirring. During the addition, the mixture was adjusted to pH 5 to 5.5 with 10% aqueous sodium hydroxide, and the stirring was continued at ambient temperature for 2 hours. After removal of the ethanol, to the remaining aqueous solution was added ethyl acetate, followed by adjusting to pH 7.5 with 10% aqueous sodium hydroxide. The aqueous layer was separated and washed with ethyl acetate. Thereto was further added ethyl acetate, followed by adjusting to pH 2.0 with 10% hydrochloric acid. The separated organic layer was washed with a saturated aqueous sodium chloride and then dried over magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with n-hexane and tetrahydrofuran, and collected by filtration. To this substance were added ethanol (50 ml) and water (30 ml), followed by adjusting to pH 7.5 with 10% aqueous sodium hydroxide. The precipitated substance was collected by filtration, washed with a mixed solvent of water and ethanol (1:1 by volume), followed by addition of water and ethyl acetate, and adjusting to pH 2.0 with 10% hydrochloric acid. The organic layer was separated, washed with a saturated aqueous sodium chloride and then dried over magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with n-hexane and tetrahydrofuran to obtain 2-(trans-3-tert-butoxycarbonylallyloxyimino)-2-(2-formamidothiazol-4-yl)acetic acid (syn isomer) (12.01 g). IR (Nujol) : 3150, 1720, 1650 cm⁻¹ NMR δppm (DMSO-d₆) : 1.47 (9H, s), 4.89 (2H, m), 5.96 (1H, m), 6.69-7.16 (1H, m), 7.60 (1H, s), 8.57 (1H, s), 12.72 (1H, broad s) Example 2 To a suspension of (2-formamidothiazol-4-yl)-glyoxylic acid (3.6 g) in pyridine (3.7 ml) and water (33 ml) was added a solution of L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethyl 2-aminooxyacetate (10.5 g) in tetrahydrofuran (30 ml), and the mixture was stirred at ambient temperature for 5 hours. Water was added to the reaction mixture, followed by adjusting to pH 1.6 with conc. hydrochloric acid. After extraction with ethyl acetate, the extract was washed with an aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain 2-(2-formamidothiazol-4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethoxycarbonylmethoxyimino)acetic acid (syn isomer)(11.7 g), mp 110-113°C. IR (Nujol) : 3330, 3180, 1756 (shoulder), 1743, 1715, 1703 cm⁻¹ NMR δppm (DMSO-d₆) : 1.38 (9H, s), 4.47 (3H, broad s), 4.70 (2H, broad s), 6.85 (1H, s), 7.40 (10H, broad s), 7.55 (1H, s), 8.55 (1H. s), 12.70 (1H, broad s) Example 3 To a suspension of 2-(2-formamidothiazol-4-yl)-glyoxylic acid (3.40 g) in pyridine (3.6 ml) and water (32 ml) was added a solution of benzhydryl DL-2-tert-butoxycarbonylamino-4-aminooxybutyrate (7.0 g) in tetrahydrofuran (30 ml) at ambient temperature, and the mixture was stirred for 3 hours. The reaction mixture was poured into ethyl acetate (200 ml), and the separated ethyl acetate layer was washed with dilute hydrochloric acid and an aqueous sodium chloride, followed by drying over anhydrous magnesium sulfate. Removal of the solvent gave 2-(2-formamidothiazol-4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino)acetic acid (syn isomer)(9.0 g), mp 61-65°C. IR (Nujol) : 3150, 1740, 1695 cm⁻¹ NMR δppm (DMSO-d₆) : 1.4 (9H, s), 2.1 (2H, m), 4.2 (3H, m), 6.82 (1H, s), 7.33 (10H, s), 7.53 (1H, s), 8.53 (1H, s) Reference 1 To a solution of benzhydryl 7-amino-3-vinyl-3-cephem-4-carboxylate (3.02 g) in methylene chloride (30 ml) were added a solution of 2-(2-formamidothiazol4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxy-carbonylaminoethoxycarbonylmethoxyimino)acetic acid (syn isomer)(5.5 g) and then N,N'-dicyclohexylcarbodiimide (1.81 g), followed by stirring at ambient temperature for 2 hours. Diethyl ether (100 ml) was added to the reaction mixture, and the precipitated material was removed by filtration. After removing the solvent from the filtrate, the residue was dissolved in ethyl acetate, washed with 5% aqueous sodium bicarbonate and then an aqueous sodium chloride, followed by drying over anhydrous magnesium sulfate. Removal of the solvent gave a residue (10 g), which was chromatographed on silica gel (200 ml) eluting with a mixed solvent of diisopropyl ether and acetone. Fractions containing a desired compound were collected and evaporated to obtain benzhydryl 7-[2-(2-formamidothiazol-4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer)(5.9 g), mp 87-94°C. IR (Nujol) : 3350, 1780, 1720, 1700 (broad) cm⁻¹ NMR δppm (DMSO-d₆) : 1.33 (9H, s), 3.57, 3.96 (2H, ABq, J=18Hz), 4.53 (3H, m), 4.73 (2H, broad s), 5.3 (1H, d, J=11Hz), 5.33 (1H, d, J=5Hz), 5.53 (1H, d, J=18HZ), 6.00 (1H, dd, J=5Hz, 8Hz), 6.87 (1H, s), 7.00 (1H, s), 7.4 (20H, m), 7.50 (1H, s), 8.57 (1H, s), 9.80 (1H, d, J=8Hz), 12.7 (1H, broad s) Reference 2 To a solution of benzhydryl 7-amino-3-vinyl-3-cephem-4-carboxylate (3.54 g) in methylene chloride (35 ml) were added a solution of 2-(2-formamidothiazol-4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino)acetic acid (syn isomer)(6.0 g) in tetrahydrofuran (60 ml) and then N,N'-dicyclohexylcarbodiimide (2.2 g), followed by starring at ambient temperature for 4 hours. The precipitated material was removed by filtration, and the filtrate was evaporated to dryness to give a residue, which was chromatographed on silica gel eluting with a mixed solvent of diisopropyl ether and acetone. Fractions containing a desired compound were collected and evaporated to obtain benzhydryl 7-[2-(2-formamidothiazol-4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer)(3.80 g), mp 153-158°C. IR (Nujol) : 3200, 1780, 1700 (broad) cm⁻¹ NMR δppm (DMSO-d₆) : 1.36 (9H, s), 2.1 (2H, m), 3.6 (2H, m), 4.2 (3H, m), 5.2-6.1 (4H, m), 6.8 (1H, s), 6.97 (1H, s), 6.8-7.2 (1H, m), 7.37 (20H, m), 7.43 (1H, s), 8.53 (1H, s), 9.75 (1H, d, J=8Hz), 12.7 (1H, broad s) Reference 3 To a solution of benzhydryl 7-[2-(2-formamidothiazol-4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephen-4-carboxylate (syn isomer)(6.9 g) in methanol (140 ml) was added conc. hydrochloric acid (3.1 ml), and the mixture was stirred at 35°C for 90 ninutes. The reaction mixture was adjusted to pH 6.0 with 5% aqueous sodium bicarbonate and then diluted with water (200 ml). Removal of the methanol gave an aqueous solution, which was extracted with ethyl acetate. The extract was washed with an aqueous sodium chloride and then dried over anhydrous magnesium sulfate, followed by removal of the solvent. The residue was pulverized with diisopropyl ether and collected by filtration to obtain benzhydryl 7-[2-(2-aminothiazol-4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (5.5 g), mp 124-128°C. IR (Nujol) : 3360, 1750 (broad) cm⁻¹ NMR δppm (DMSO-d₆) : 1.40 (9H, S), 3.57, 3.98 (2H, ABq, J=18Hz), 4.50 (3H, m), 4.63 (2H, broad s), 5.30 (1H, d, J=11Hz), 5.31 (1H, d, J=5Hz), 5.67 (1H, d, J=18Hz), 5.95 (1H, dd, J=5Hz, 8Hz), 6.86 (2H, s), 6.8-7.20 (1H, m), 7.00 (1H, s), 7.40 (10H, s), 9.65 (1H, d, J=8Hz) Reference 4 To a solution of benzhydryl 7-[2-(2-formamidothiazol4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino) acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer)(3.65 g) in methanol (109 ml) was added conc. hydrochloric acid (1.96 ml), and the mixture was stirred at 35°C for 24 minutes. The reaction mixture was adjusted to pH 6.5 with 10% aqueous sodium hydroxide and 5% aqueous sodium bicarbonate, followed by removal of the methanol. The residue was dissolved in ethyl acetate, washed with an aqueous sodium chloride and then dried over anhydrous magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain benzhydryl 7-[2-(2-aminothiazol-4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino) acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (3.5 g), mp 119-122°C. IR (Nujol) : 3300, 1780, 1719, 1680 cm⁻¹ NMR δppm (DMSO-d₆) : 1.37 (9H, s), 2.1 (2H, m), 3.7 (2H, m), 4.2 (3H, m), 5.2-6.1 (4H, m), 6.8 (2H, s), 6.8-7.2 (1H, m), 6.97 (1H, s), 7.37 (20H, s), 9.67 (1H, d, J=8Hz) Reference 5 A mixture of benzhydryl 7-[2-(2-aminothiazol-4-yl)-2-(L-2-benzhydryloxycarbonyl-2-tert-butoxycarbonylaminoethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer)(5.4 g), trifluoroacetic acid (21.6 ml) and anisole (5.4 ml) was stirred at 5 to 10°C for 70 minutes. The reaction mixture was added dropwise to diisopropyl ether (1000 ml), followed by collecting the precipitated material. After washing with diisopropyl ether, said material was dissolved in a mixture of ethyl acetate (50 ml) and water (50 ml). The separated aqueous solution was washed with ethyl acetate, and the ethyl acetate therein was completely removed by evaporation. The resultant aqueous solution was adjusted to pH 3.0 with 5% sodium bicarbonate and then chromatographed on a nonionic adsorption resin, "Diaion HP-20" (100 ml). After washing with water (300 ml), elution was carried out 20% aqueous isopropyl alcohol, and fractions containing a desired compound were collected. Removal of the solvent gave a residue, which was lyophilized to obtain 7-[2-(2-aminothiazol-4-yl)-2-(L-2-amino-2-carboxyethoxycarbonylmethoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid (syn isomer)(1.7 g), mp 158°C (dec.). IR (Nujol) : 3200 (broad), 1760 (broad) cm⁻¹ NMR δppm (DMSO-d₆) : 3.51, 3.75 (2H, ABq, J=18Hz), 4.3-4.8 (5H, m), 5.13 (1H, d, J=5Hz), 5.19 (1H, d, J=11Hz), 5.44 (1H, d, J=18Hz), 5.72 (1H, dd, J=5Hz, 8Hz), 6.79 (1H, s), 6.93 (1H, dd, J=11Hz, 18Hz), 9.66 (1H, d, J=8Hz) Reference 6 A mixture of benzhydryl 7-[2-(2-aminothiazol-4-yl)-2-(DL-3-benzhydryloxycarbonyl-3-tert-butoxycarbonylaminopropoxyimino) acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer)(3.3 g), trifluoroacetic acid (20 ml) and anisole (3.3 ml) was stirred at 5 to 10°C for 1.5 hours. The reaction mixture was added dropwise to diisopropyl ether (300 ml), and the precipitated material was collected by filtration and then washed with diisopropyl ether, followed by dissolving in water (50 ml). The aqueous solution was washed with ethyl acetate (50 ml x 2), and the ethyl acetate therein was completely removed by evaporation. The resultant aqueous solution was adjusted to pH 3.1 with 5% aqueous sodium bicarbonate and chromatographed or a nonionic adsorption resin, "Diaion HP-20" (100 ml). After washing with water (300 ml), elution was carried out with 20% aqueous isopropyl alcohol, and the fractions containing a desired compound were collected and then treated with an activated charcoal. Removal of the solvent gave a residue, which was lyophilized to obtain 7-[2-(2-aminothiazol-4-yl)-2-(DL-3-amino-3-carboxypropoxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid (syn isomer)(0.7 g), mp 145°C (dec.). IR (Nujol) : 3120, 1766, 1612 cm⁻¹ NMR δ ppm (D₂O+DCl) : 2.53 (2H, m), 3.82 (2H, broad s), 4.31 (1H, t, J=6Hz), 4.57 (2H, t, J=6Hz), 5.32 (1H, d, J=5Hz), 5.53 (1H, d, J=11Hz), 5.73 (1H, d, J=18Hz), 5.82 (1H, d, J=5Hz), 7.13 (1H, dd, J=11Hz, 18Hz), 7.25 (1H, s) Reference 7 Benzhydryl 7-amino-3-vinyl-3-cephem-4-carboxylate hydrochloride (3.0 g) was dissolved in dried ethyl acetate (50 ml) and trimethylsilylacetamide (6.4 g) at 40°C. On the other hand, to a Vilsmeier reagent, which was prepared by reacting dried N,N-dimethylformamide (0.66 g) with phosphorus oxychloride (1.39 g)in dried ethyl acetate (2.64 ml) in a conventional manner, were added dried tetrahydrofuran (25 ml) and 2-(trans-3-tert-butoxycarbonylallyloxyimino)-2-(2-formamidothiazol-4-yl)acetic acid (syn isomer) (2.7 g), followed by stirring at -3 to 3°C for a while to prepare the activated acid solution. This solution was added to the ethyl acetate solution obtained before at -10°C with stirring, and the stirring was continued at -10 to -5°C for half an hour. To the reaction mixture was added water, and the separated organic layer was washed with a saturated aqueous sodium bicarbonate and a saturated aqueous sodium chloride, followed by drying over magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain benzhydryl 7-[2-(trans-3-tert-butoxycarbonylallyloxyimino)-2-(2-formamidothiazol-4-yl)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (4.83 g). IR (Nujol) : 3250, 1780, 1710, 1660 cm⁻¹ NMR δppm (DMSO-d₆) : 1.47 (9H, s), 3.79 (2H, q, J=18.0Hz), 4.89 (2H, m), 5.34 (1H, d, J=11.0Hz), 5.35 (1H, d, J=5 0Hz), 5.68 (1H, d, J=18.0Hz), 5.86-6.30 (2H, m), 6.52-7.22 (2H, m), 7.00 (1H, s), 7.21-7.74 (11H, m), 8.58 (1H, s), 9.91 (1H, d, J=8.0Hz), 12.73 (1H, broad s) Reference 8 A mixture of benzhydryl 7-[2-(trans-3-tert-butoxycarbonylallyloxyimino)-2-(2-formamidothiazol-4-yl)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (4.7 g), conc. hydrochloric acid (1.34 g), methanol (30 ml) and tetrahydrofuran (10 ml) was stirred at ambient temperature for 2.5 hours. To the reaction mixture was added ethyl acetate, followed by adjusting to pH 7.5 with a saturated aqueous sodium bicarbonate. The separated organic layer was washed with a saturated aqueous sodium chloride and then dried over magnesium sulfate. Removal of the solvent gave a residue, which was pulverized with diisopropyl ether to obtain benzhydryl 7-[2-(trans-3-tert-butoxycarbonylallyloxyimino)-2-(2-aminothiazol-4-yl)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (4.50 g). IR (Nujol) : 3250, 1770, 1700, 1670, 1610 cm⁻¹ NMR δppm (DMSO-d₆) : 1.45 (9H, s), 3.76 (2H, m), 4.86 (2H, m), 5.34 (1H, d, J=12.0Hz), 5.35 (1H, d, J=5.0Hz), 5.68 (1H, d, J=18.0Hz), 5.77-6.30 (2H, m), 6.54-7.17 (2H, m), 6.86 (1H, s), 7.00 (1H, s), 7.17-7.70 (10H, m), 9.81 (1H, d, J=8 0Hz) Reference 9 To a solution of benzhydryl 7-[2-(2-aminothiazol-4-yl)-2-(trans-3-tert-butoxycarbonylallyloxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylate (syn isomer) (4.4 g) and anisole (4.4 ml) in methylene chloride (9.0 ml) was added trifluoroacetic acid (17.6 ml) under ice-cooling, and the mixture was stirred at ambient temperature for 2 hours. To the reaction mixture was added diisopropyl ether, and the precipitated substance was collected by filtration, which was washed with diisopropyl ether. To this substance were added ethyl acetate and water, and then adjusted to pH 7.5 with a saturated aqueous sodium bicarbonate. The separated aqueous layer was washed with ethyl acetate, and the remaining ethyl acetzte in the aqueous solution was completely removed by evaporation, followed by adjusting to pH 2.2 with 10% hydrochloric acid. The precipitated substance was collected by filtration and then dried to obtain 7-[2-(2-aminothiazol-4-yl)-2-(trans-3-carboxyallyloxyimino)acetamido]-3-vinyl-3-cephem-4-carboxylic acid (syn isomer) (2.31 g). IR (Nujol) : 3250, 1760, 1690, 1650 cm⁻¹ NMR δppm (DMSO-d₆) : 3.73 (2H, q, J=16.0Hz), 4.84 (2H, m), 5.24 (1H, d, J=4.0Hz), 5.34 (1H, d, J=12.0Hz), 5.47-6.23 (3H, m), 6.63-7.34 (2H, m), 6.83 (1H, s), 9.77 (1H, d, J=8.0Hz)

Claims: Carboxylic acid derivative of the formula in which R¹ is aminothiazolyl which may have halogen, aminothiadiazolyl, protected aminothiazolyl which may have halogen or protected aminothiadiazolyl, R 4 / h is C₂-C₇ alkenyl substituted by carboxy or a protected carboxy group, C₁-C₇ alkyl substituted by a protected amino and a protected carboxy groups, or C₁-C₇ alkyl substituted by a protected amino- and a protected carboxy-substituted-(C₁-C₇)alkoxycarbonyl, and R e is carboxy or a protected carboxy group, or a salt thereof, A process for preparing carboxylic acid derivatives of the formula: in which R¹ is aminothiazolyl which may have halogen, aminothiadiazolyl, protected aminothiazolyl which may have halogen or protected aminothiadiazolyl, R 4 / h is C₂-C₇ alkenyl substituted by carboxy or a protected carboxy group, C₁-C₇ alkyl substituted by a protected amino and a protected carboxy groups, or C₁-C₇ alkyl substituted by a protected amino- and a protected carboxy-substituted-(C₁-C₇)alkoxycarbonyl, and R e is carboxy or a protected carboxy group, or a salt thereof, which comprises reacting a compound of the formula R¹-CO-R e R¹ and R e are each as defined above, or a salt thereof with a compound of the formula R 4 / hONH₂ in which R 4 / h is as defined above, or a salt thereof.

Inventors: KAWABATA, KOHJI, MASUGI, TAKASHI, TAKASUGI, HISASHI, TAKAYA, TAKAO, YAMANAKA, HIDEAKI
Applicants: FUJISAWA PHARMACEUTICAL CO., LTD.
Application year: 1980
Main classification: C07D277
IPC codes: A61P31, C07D501, C07D213, C07D277, C07D417, C07D239, A61K31, C07F9, C07D257, C07D271, C07D285, C07D209