1,2,4-TRIAZINE DERIVATIVES, PROCESS FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION

Abstract: A compound of the formula: wherein Ar is a phenyl group substituted with halogen, C₁-C₈ alkyl, C₁-C₈ alkoxy, C₁-C₈ alkylthio, C₁-C₈ alkylsulfonyl, nitro, trihalomethyl, cyano, C₁-C₈ alkylamino, di(C₁-C₈)-alkylamino, amino, hydroxyl, phenyl and/or phenoxy, a naphthyl group or a thienyl group optionally substituted with halogen which is useful as an antifungal agent.

Description: 1,2,4-Triazine -Derivatives, Process for their Production, Pharmaceutical Composition and their Use. The present invention relates to 1,2,4-triazine derivatives, and their production and use. More particularly, it relates to 1,2,4-triazine derivatives of the formula: EMI1.1 wherein Ar is a phenyl group substituted with halogen, C1-C8 alkyl, C1-C8 alkoxy, C1-C8 alkylthio, C1-C8 alkylsulfonyl, nitro, trihalomethyl, cyano, C1-C8 alkylamino, di(C1-C8)- alkylamino, amino, hydroxyl, phenyl and/or phenoxy, a naphthyl group or a thienyl group optionally substituted with halogen, their preparation process and their antifungal use. In the above significances, the alkyl moiety may be straight or branched, and can cover methyl, ethyl, npropyl, iso-propyl, n-butyl, iso-butyl, tert-butyl, npentyl, n-hexyl, n-heptyl, n-octyl, etc. Among them, those having 1 to 4 carbon atoms are preferred. The number of the substituent(s) which should be present on the phenyl group or may be present on the thienyl group is usually not more than 3. The said 1,2,4-triazine derivatives (I) can be prepared, for instance, by reacting a keto-carboxylic acid or its ester of the formula: EMI2.1 wherein R is a hydrogen atom or a C1-Co alkyl group and Ar is as defined above, with 2-pyridylhydrazidine of the formula: EMI2.2 The keto-carboxylic acid or its ester (II) is known or can be easily prepared by a pQr se known procedure as described in "Organic Syntheses" Coll. Vol. 1, 241 (1941). The 2-pyridylhydrazidine (III) is known and described in J.Org.Chem., 30, 931 (1955). The reaction is readily carried out by treating the keto-carboxylic acid or its ester (II) with 2-pyridylhydrazidine (III), usually in an equimolar proportion. It is particularly preferred to effect the treatment in an inert solvent such as an alkanol (e.g. methanol, ethanol), an ether (e.g. tetrahydrofuran, dioxane), a halogenated hydrocarbon (e.g. carbon tetrachloride, methylene chloride), dimethylformamide or water at a temperature between OOC and 3000C, especially between 60QC and 2000C. The said reaction between the keto-carboxylic acid or its ester (II) and 2-pyridylhydrazidine (III) may be considered to proceed through the following intermediary compound: EMI3.1 wherein Ar and R are each as defined above. When the ketoextbaxylic acid (II: R = H) is used as the starting material, its reaction with 2-pyridylhydrazidine (III) at a temperature not higher than room temperature can afford the corresponding compound (IV: R = H), which may be heated, with or without isolation from the reaction mixture, above room temperature to give the objective 1,2,4-triazine derivative (I) with ease. When the keto-carboxylic acid (II: R = H) is reacted with 2-pyridylhydrazidine (III) under heating, the corresponding intermediary compound (IV: R = H) is hardly isolated, and the objective 1,2,4-triazine derivative (I) is directly produced. In case of the starting material being the keto-carboxylic ester (II: R = C1-C4 alkyl), the corresponding intermediary compound (IV: R = C1-C4 alkyl) is hardly produced even when the reaction is effected at low temperature, and there is obtained directly the objective 1,2,4-triazine derivative (I). The 1,2,4-triazine derivatives (I) of the invention are novel and exhibit a strong antifungal activity against pathogenic fungi as shown in Table 1: Table 1: Antifungal activity in vitro EMI4.1 <tb> <SEP> 1,2,4-Triazine <SEP> ! <SEP> Minimal <SEP> inhibitory <SEP> concentration <SEP> (g/ml) <tb> <SEP> derivative <SEP> (I) <tb> <SEP> 0 <SEP> I <SEP> Trichophyton <SEP> rubrum <SEP> Candida <SEP> albicans <tb> <SEP> -Ar <tb> <SEP> Ar <tb> 10 <SEP> F <SEP> b <SEP> j <SEP> 10 <SEP> 20 <SEP> - <SEP> 100 <tb> i <SEP> F\ <tb> 1.25 <SEP> 20 <tb> jW <SEP> F <SEP> j <SEP> 0.6 <tb> <SEP> 4 <tb> <SEP> 20 <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> C1 <SEP> t <SEP> 0.6 <SEP> 20 <tb> <SEP> I <SEP> II <tb> <SEP> t <tb> <SEP> 0.6 <SEP> 10 <tb> <SEP> 0.6 <SEP> 20 <tb> <SEP> ;c-Br <tb> <SEP> 0.6 <SEP> r <SEP> 20 <SEP> - <SEP> 100 <tb> . <SEP> I <tb> <SEP> 3 <SEP> 100 <tb> i <SEP> 2.5 <SEP> 100 <tb> (Continued) EMI5.1 <tb> <SEP> 1,2,4-Triazine <SEP> I <SEP> Minimal <SEP> inhibitory <SEP> concentration <SEP> (-Clg/ml) <tb> <SEP> derivative <SEP> (I) <tb> <SEP> I <tb> <SEP> Ar <tb> <SEP> Ar <SEP> 20 <SEP> - <SEP> 100 <SEP> 1 <SEP> 100 <tb> <SEP> · <tb> <SEP> C1 <SEP> g <tb> <SEP> 1 <tb> <SEP> C1 <SEP> f <SEP> 2.5 <SEP> ', <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> L <SEP> t-C1 <tb> <SEP> µ <SEP> 0.3 <SEP> 20 <SEP> - <SEP> 100 <tb> X <SEP> C1 <SEP> '. <tb> <SEP> 1 <SEP> 1.25 <SEP> 20 <SEP> - <SEP> 100 <tb> kCH3 <tb> <SEP> zi <SEP> 3 <tb> <SEP> e <SEP> 3 <SEP> 1 <SEP> 2.5 <SEP> 20 <SEP> - <SEP> 100 <tb> f <SEP> I <tb> <SEP> /OCH3 <SEP> 20 <SEP> 1 <SEP> 20 <SEP> j <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> ¸/OCH3 <SEP> | <tb> <SEP> \zJ <SEP> 3 <SEP> -1 <SEP> I <tb> S <SEP> e <SEP> i <SEP> 2.5 <SEP> 2.5 <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> ¯¯, <SEP> SCH3 <tb> Z <SEP> 20 <SEP> - <SEP> 100 <SEP> 100 <tb> <SEP> ISO2CH3 <tb> <SEP> NO, <SEP> 20 <SEP> - <SEP> 100 <SEP> 100 <tb> (Continued) EMI6.1 <tb> <SEP> 1,2,4-Triazine <SEP> Minimal <SEP> inhibitory <SEP> concentration <SEP> (g/ml) <tb> <SEP> 1 <SEP> derivative <SEP> (I) <tb> <SEP> 1 <SEP> I <tb> <SEP> H <SEP> O <SEP> Trichophyton <SEP> rubrum <SEP> Candida <SEP> albicans <tb> <SEP> N <SEP> 8 <tb> <SEP> ,, <tb> <SEP> e <SEP> NO2 <SEP> I <SEP> - <SEP> 20 <SEP> 1 <SEP> 100 <SEP> <tb> <SEP> I <tb> <SEP> 4 <SEP> 2.5 <SEP> 5 <tb> <SEP> 1.25 <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> 'CF3 <tb> - <SEP> <tb> <SEP> ¹CN <SEP> 1.25 <SEP> -20 <SEP> -,100 <tb> <SEP> : <SEP> ,CH, <SEP> 2,5 <SEP> 100 <tb> <SEP> e <SEP> ZCH3 <SEP> í <SEP> 2.5 <SEP> , <SEP> 100 <tb> <SEP> CH3 <tb> <SEP> 1 <SEP> i <SEP> 20 <SEP> - <SEP> 100- <SEP> I <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> I <tb> <SEP> 1 <SEP> 20 <SEP> I <tb> <SEP> 20 <SEP> N2 <SEP> µ <SEP> 20 <SEP> 1 <SEP> 20 <SEP> - <SEP> 100 <tb> <SEP> - <SEP> 100 <tb> <SEP> 5 <SEP> 100 <tb> <SEP> 1 <tb> <SEP> 0, <SEP> 20 <SEP> 20 <tb> <SEP> d <SEP> o <SEP> < <SEP> 20 <SEP> 1 <SEP> 20 <tb> <SEP> < <SEP> 20 <SEP> 20 <tb> (Continued) EMI7.1 <tb> <SEP> 1,2,4-Triazine <SEP> Minimal <SEP> inhibitory <SEP> concentration <SEP> (Zg/ml) <tb> <SEP> derivative <SEP> (I) <tb> r <SEP> Trichophyton <SEP> rubrum <SEP> : :Candida <SEP> albicans <SEP> i <tb> <SEP> 1 <tb> f/N-Ar <tb> <SEP> 1 <tb> <SEP> Ar <tb> <SEP> 20 <SEP> < < <SEP> 1 <SEP> 20 <SEP> 100 <tb> <SEP> 0.6 <SEP> ffi <SEP> 2 <SEP> 20 <tb> <SEP> II <tb> <SEP> S <tb> Z <SEP> Z <SEP> 20 <SEP> Z <SEP> 20 <SEP> Z <tb> <SEP> 1 <SEP> 20 <SEP> 20 <tb> <SEP> 2.5 <SEP> 100 <tb> <SEP> Br <tb> #3r <SEP> 2.5 <SEP> . <SEP> 100 <tb> Thus, the 1,2,4-triazine derivatives (I) of the invention are useful as antifungal agent. They can be administered parenterally, orally or locally. to warm-blooded animals in the form of conventional pharmaceutical preparations. For instance, they can be administered in the form of conventional sblid pharmaceutical preparations such as tablets, capsules, powders or granules, or in the form of conventional liquid pharmaceutical preparations such as suspensions, emulsions or solutions. The daily dosage may vary depending upon the administration route and is usually between 10 mg to 5 g. Practical and presently preferred embodiments of the invention are illustratively shown in the following Examples, which are not intended to limit the scope of the invention thereto. Example 1 A mixture of 0.68 g (0.005 mol) of 2-pyridylhydrazidine, 0.95 g (0.005 mol) of 4-chlorophenylglyoxylic acid and 10 ml of ethanol was stirred at room temperature for 15 hours, and precipitated crystals were collected by filtration. The crystals were dissolved in 10 ml of dimethylformamide, and the resulting solution was stirred at 100 to 1200C for 4 hours. The solvent was evaporated off, and the residue was recrystallized from ethanol to give 0.95 g of 6-(4-chlorophenyl)-3-(2-pyridyl)-1,2,4-triazin-5(4E)- one as yellow needles melting at 216 - 2170C. Elementary analysis for C14HgN4OCl (%): Calcd.: C, 59.06, H, 3.19; N, 19.68; Found: C, 59.20; H, 3.00; N, 19.96. Example 2 A mixture of 0.68 g (0.005 mol) of 2-pyridylhydrazidine, 0.95 g (0.005 mol) of 4-chlorophenylglyoxylic acid and 50 ml of ethanol was heated under reflux for 5 hours. The reaction mixture was cooled, and precipitated crystals of 6- (4-chlorophenyl)-3- (2-pyridyl)-l,2,4-triazin- 5(4H)-one were collected by filtration. The infrared spectrum and melting point of the crystals were consistent with those of the product in Example 1. Example 3 A mixture of 0.68 g (0.005 mol) of 2-pyridylhydrazidine, 1.06 g (0.005 mol) of ethyl 4-chlorophenylglyoxylate and 50 ml of ethanol was heated under reflux for 5 hours. The reaction mixture was filtered while hot to remove 5-(4-chlorophenyl)-3-(2-pyridyl)-1,2,4-triazin-6(1H)- one, which is an isomer of the desired product. The filtrate was cooled, and precipitated crystals of 6-(4chlorophenyl)-3-(2-pyridyl)-1,2,4-triazin-5(4H)-one was collected by filtration. The infrared spectrum and melting point of the crystals were consistent with those of the product in Example 1. Example 4 A mixture of 0.68 g (0.005 mol) of 2-pyridylhydrazidine, 1.06 g (0.005 mol) of ethyl 4-chlorophenylglyoxylate and 50 ml of ethanol was stirred under ice cooling for 5 hours and at room temperature for 10 hours. Crystals of 6- (4-chlorophenyl)-3- (2-pyridyl)-l,2,4-triazin- 5(4H)-one were precipitated from the reaction mixture and collected by filtration. The infrared spectrum and melting point of the crystals were consistent with those of the product in Example 1. In the same manner as in Example 1, the following compounds were obtained: 6-(2-Chlorophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 168 - 1690C; 6-(3-Chlorophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 186 - 1870C; 6-(2-Fluorophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 178 - 1810C; 6-(3-Fluorophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 198 - 199 C; 6- (4-Fluorophenyl) -3- (2-pyridyl) -1,2,4-triazin- 5(4H)-one, M.P., 191 - 1920C; 6-(4-Bromophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P. 222 - 2240C; 6-(4-Iodophenyl)-3-(2-pyridyl)-1,2,4-triazin 5(4H)-one, M.P. 224 - 2250C; 6-(2,4-Dichlorophenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M P., 214 - 2150-C; ; 6- (3 ,4-Dichlorophenyl)-3- (2-pyridyl) -1,2,4- triazin-5(4H)-one, M.P. 249 - 2500C; 6- (2,4-Difluorophenyl)-3- (2-pyridyl)-l,2,4- triazin-5(4H)-one, M.P. 217 - 218 C; 6-(2-Chloro-6-fluorophenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P. 199 - 2020C; 6- (4-Methylphenyl)-3- (2-pyridyl) -1,2,4-triazin5(4H)-one, M.P. 165 - 1660C; 6-(4-Methoxyphenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 184 - 1850C; 6-(4-Methylthiophenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P., 188 - 1900C; 6-(4-Mesylphenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 252 - 2530C; 6-(3-Nitrophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 289 - 2900C; 6- (4-Nitrophenyl)-3- (2-pyridyl) -1,2,4-triazin- 5(4H)-one, M.P., 243 - 2450C; ; 6-(3-Trifluoromethylphenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P., 178 - 1810C; 6-(4-Trifluoromethylphenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P., 231 - 2320C; 6- (4-Cyanophenyl) -3- (2-pyridyl) -1,2,4-triazin- 5(4H)-one, M.P., 252 - 2540C; 6-(4-Dimethylaminophenyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P., 2500C; 6-(3,4-Dimethoxyphenyl)-3- (2-pyridyl)-l,2,4- triazin-5(4H)-one, M.P., 193 - 1950C; 6-(4-Hydroxyphenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P. 2100C; 6-(4-Aminophenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P. 215 - 2170C; 6- (4-Phenylphenyl) -3- (2-pyridyl) -1,2, 4-triazin- 5(4H)-one, M.P. 200 - 2030C; ; 6-(4-Phenoxyphenyl)-3-(2-pyridyl)-1 r 2,4-triazin- 5(4H)-one, M.P., 178 - 1810C; 6-3-Phenoxyphenyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 146 - 1480C; 6-(2-Naphthyl)-3-(2-pyridyl)-1,2,4-triazin-5 (4H) - one, M.P., 185 - 1860C; 6-(2-Thienyl)-3-(2-pyridyl)-1,2,4-triazin-5(4H)- one, M.P., 220 - 2220C; 6-(5-Chloro-2-thienyl)-3-(2-pyridyl)-1,2,4- triazin-5(4H)-one, M.P., 217 - 219 C;. 6-(3-Bromo-2-thienyl)-3-(2-pyridyl)-1,2,4-triazin- 5(4H)-one, M.P., 198 - 2010C.

Claims: What is claimed is: 1. 1,2,4-Triazine derivatives of the formula: EMI13.1 wherein Ar is a phenyl group substituted with halogen, C1-C8 alkyl, C1-C8 alkoxy, C1-C8 alkylthio, C1-C8 alkylsulfonyl, nitro, trihalomethyl, cyano, C1-C8 alkylamino, di(C1-C8)- alkylamino, amino, hydroxyl, phenyl and/or phenoxy, a naphthyl group or a thienyl group optionally substituted with halogen. 2. The compound according to claim 1, wherein Ar is a phenyl group substituted with halogen. 3. The compound according to claim 2, wherein Ar is 3-fluorophenyl. 4. The compound according to claim 2, wherein Ar is 4-fluorophenyl. 5. The compound according to claim 2, wherein Ar is 3-chlorophenyl. 6. The compound according to claim 2, wherein Ar is 4-chlorophenyl. 7. The compound according to claim 2, wherein Ar is 3,4-dichlorophenyl. 8. The compound according to claim 2, wherein Ar is 4-bromophenyl. 9. The compound according to claim 2, wherein Ar is 4-iodophenyl. 10. The compound according to claim 1, wherein Ar is a phenyl group substituted with C1-C8 alkyl. 11. The compound according to claim 10, wherein Ar is 4-methylphenyl. 12. The compound according to claim 1, wherein Ar is a phenyl group substituted with trihalomethyl. 13. The compound according to claim 12, wherein Ar is 3-trifluoromethyl. 14. The compound according to claim 1, wherein Ar is a thienyl group. 15. A process for producing 1,2,4-triazine derivatives of the formula: EMI15.1 wherein Ar is a phenyl group substituted with halogen, C1-C8 alkyl, C1-C8 alkoxy, C1-C8 alkylthio, C1-C8 alkylsulfonyl, nitro, trihalomethyl, cyano, C1-C8 alkylamino, di(Cl-C8)- alkylamino, amino, hydroxyl, phenyl and/or phenoxy, a naphthyl group or a thienyl group optionally substituted with halogen, which comprises reacting a compound of the formula: EMI15.2 wherein R is a hydrogen atom or a C1-C4 alkyl group and Ar is as defined above, with a compound of the formula: EMI15.3 16. Apharmaceutical composition which comprises as an active ingredient an effective amount of the compound as claimed in claim 1. 17. Use of the compound as claimed in claim 1 for the preparation of a pharmaceutical composition having an antifungal effect.

Inventors: AGUI, HIDEO, AONO, SHUNJI, OKUDA, TAKAO, TAMOTO, KATSUMI
Applicants: SUMITOMO CHEMICAL COMPANY, LIMITED
Application year: 1980
Main classification: C07D401
IPC codes: C07D409, C07D401