SUBSTITUTED 6-PHENETHYL-AND PHENYLETHENYL-3,4,5,6-TETRAHYDRO-4-HYDROXYTETRAYDROPYRAN-2-ONES IN THE4-R TRANS STEREOISOMERIC FORMS AND THE CORRESPONDING DIHYDROXY ACIDS, PROCESS FOR PREPARING AND PHARMACEUTICAL COMPOSITION COMPRISING THEM

Abstract: 6-Phenyl, phenalkyl- and phenylethenyl-4-hydroxytetra­ hydropyran-2-ones in the 4- R trans stereoisomeric forms hav­ ing the structure wherein A is H or methyl; E is a direct bond, -CH₂-, -CH₂-CH₂-, -CH₂-CH₂-CH₂- or -CH=CH-; and R₁, R₂ and R₃ have several meanings, and the corresponding dihydroxy acids in which the lactone ring is hydrolytically opened, and the pharmaceutically accep­ table salts of said dihydroxy acids, and the lower alkyl and the phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; and processes for preparing them. These compounds are po­ tent inhibitors of cholesterol synthesis by the enzyme hydroxy­ methyl glutaryl coenzyme A reductase.

Description: 6-PHENYL-, PHENALKYL- AND PHENYLETHENYL-4 HYDROXYTETRAHYDROPYRAN-2-ONES IN THE 4- R TRANS STEREOISOMERIC FORMS, PROCESS FOR PREPARING AND PHARMACEUTICAL COMPOSITION COMPRISING THEM This invention relates to new hypocholesterolemic and hypolipemic compounds having the structure (I) EMI1.1 wherein A is H or methyl; E is a direct bond, CH2- , -CIR2-CX-, -CH2-CH2-CH2- or -CH=CH-; ; R1, R2 and R3 are each selected from H, halogen, C1-4 alkyl, C1-4 haloalkyl, phenyl, phenyl substituted by halogen, C1-4 alkoxy C2-8 alkanoyloxy C1-4 alkyl, or C1-4 haloalkyl, and OR4 in which R4 is H, C28 alkanoyl, benzoyl, phenyl, halophenyl, phenyl C1-3 alkyl, C1-9 alkyl, cinnamyl, C1-4 haloalkyl, allyl, ycloalkyl-C1-3-alkyl, adamantyl-C1-3-alkyl, or substituted phenyl C1 3-alkyl in each of which the substituents are selected from halogen, C1-4 alkoxy C1-4 alkyl, or C1,4 haloalkyl; and the corresponding dihydroxy acids resulting from the hydrolytic opening of the lactone ring, and the pharmaceutically acceptable salts of said acids, and the lower alkyl and phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the enantiomers having a 4 R configuration in the tetrahydropyran moiety of the trans racemate shown in formula I. A preferred embodiment of this invention relates to those structures of general formula I wherein A is H or methyl; E is -CH=CH-, -CH2CH2- or -CH2CH2CH-; R1, R2 and R3 are each selected from halogen, c14 alkyl, C1-4 haloalkyl, phenyl or substituted phenyl in which the substituent is halo, C1-4 alkyl, C1-4 alkoxy, and R40 in which R4 is phenyl, halophenyl, or substituted phenyl-C13-alkyl wherein the substituents are selected from halogen and C1-4 haloalkyl; and the corresponding dihydroxy acids resulting from the hydrolytic opening of the lactone ring and the pharmaceutically acceptable salts of said dihydroxy acids, and the lower alkyl and phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the enantiomer having a 4 R configuration in the tetrahydropyran moiety of the trans racemate shown in general fromula I. The most preferred embodiment of the present invention comprises those structures of general formula I wherein A is H or methyl; E is -CH2CH2- or -CH=CH-; R1 is situated ortho to E and is halophenyloxy, halophenylalkoxy or halophenyl, especially where halo is fluoro or chloro; R2 and R3 are halo, especially chloro, in the 4 and 6 positions; and the corresponding dihydroxy acids resulting from the hydrolytic opening of the lactone ring, and the pharmaceutically acceptable salts of said dihydroxy acids, and the lower alkyl and phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the enantiomer having a 4 R configuration in the tetrahydropyran moiety of the trans racemate shown in general formula I. The compounds in which R1 is halophenyl, especially fluoroph-nyl and chlorophenyl, are especially to be preferred. The designation 4 R with respect to these compounds indicates that the absolute configuration in space at the 4-carbon of the pyranone ring is believed to be the Rectus (R). series. All the compounds synthesized have been found to be dextrorotatory. BACKGROUND OF THE INVENTION It is known that certain mevalonate derivatives inhibit the biosynthesis of cholesterol, cf F.M. Singer, et al., Proc. Soc. Exper. Biol. Med., 102, 270 (1959) and F. H. Hulcher, Arch. Biochem. Biophys., 146, 422 t1971). Nevertheless, the activity of these known compounds has not always been found to be satisfactory, i.e. to have practical application. Recently, Endo et al, reported (U.S. Letters Patent 4,049,495, Patent 4,137,322 and Patent 3,983,140) the production of a fermentation product which was quite active in the inhibition of cholesterol biosynthesis. This natural product, now called compactin, was reported by Brown et al., (J. Chem. Soc. Perkin I, 1165 (1976)) to have a complex mevaloflolactone structure. However, the low rate of production from fermentation broths appears to limit the supply of this natural product. A recent Belgian patent 867,421 disclosed a group of synthetic compounds of the generic formula II EMI5.1 in which E represents a direct bond, a C13 alkylene bridge or a vinylene bridge and the various R's represent a variety of substitutents. The activity reported in the Belgian patent is less than 1% that of compactin. THE PRESENT INVENTION We have found that the enantiomers of the trans compounds of Formula I having a 4 R.configur- ation in the tetrahydropyran moiety, especially those in which A is hydrogen, and most especially those having a 2,4,6-trisubstitution pattern on the aromatic ring wherein R1 and R2 are C1 in the 2 and 4 position and R3 is phenoxy, phenylalkoxy or phenyl in the 6 position, are unexpectedly potent inhibitors of cholesterol biosynthesis, approaching and surpassing the order of magnitude of compactin. While the compounds of Formula I in which A is methyl are 4-R enantiomers of the trans racemates of the compounds of the cited Belgian patent, the latter prior art shows no recognition of the stereochemistry of these compounds, let alone the fact that an unexpectedly large improvement in the activity would result from the separation of the cis and trans racemates and the latter's resolution, especially when the preferred 2,4,6-trisubstitution occurs in the phenyl ring. However, it has been found that the 4 R enantiomers of the trans racemates corresponding to formula I specifically inhibit with high potency the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase, which is known to be the rate limiting enzyme in the process of biosynthesis of cholesterol. The inhibitory activity of these compounds for the biosynthesis of cholesterol has been measured by two methods. The experimental method A has been realized in vitro by the method of II. J. Knauss, et al., J. Biol. Chem., 234, 2835 (1959). and the activity was expressed as the molar concentration IC50 CM) necessary for the inhibition of 50% of the enzymatic activity. The experimental method B was the method of A. A. Kandutsch, et al., J. Biol. Chem., 248, 8403 (1973) for measuring the quantity of 14C-cholesterol biosynthesis from acetic acid-14C in mouse L cells. The activity is expressed for inhibition of 50% of the biosynthesis of cholesterol. The results obtained in these two assays, as reported in the cited Belgian patent, show IC50 values of 10 4 to 10-6 in both tests. The smallest 50t effective do ; cited is about 4 x 106 whereas the value for compactin, in the same tests, is about 0.8 x 10-8. We have found that the inhibitory potency is greatly increased by such isomer separation, especially when this is combined with optimal selection of a 2,4,6-arrangement of R1, R2 and R3 in the phenyl ring (i.e. selection of R1 and R2 as 2,4-dichloro and R3 as 6-phenoxy or phenylalkoxy), especially with a halogen tpreferrably fluorine) on the 6-phenoxy or phenylalkoxy group. Thus the (+) trans enantiomer of 6- [2- (.2 ,4-dichloro-6-phenylmethoxyphenyl) ethyl] - 3,4,5,6-tetrahydro 4-hydroxy-2H-pyran-2-one (Example 14) (a preferred compound of this invention) gives an IC50 of 6.8 x 10-8 in the test by method A. An even more potent and preferred compound of this invention, the (+) trans enantiomer of 6-12-(2,4-dichloro-6-E- fluorphenyl) ethenyl]-3,4,5,6-tetrahydro-4-hydroxy2H-pyran-2-one (Example 38) gives an IC50 of about 2.8 x 10 , a potency greater than that of compactin, the product described by Endo, when the two compounds are tested as the sodium salts of their corresponding hydroxy acid forms. In the compounds of formula I which form this invention, A may be hydrogen or methyl, but hydrogen is preferred. The connecting group (E) between the rings may be a direct bond, an unsaturated ethylenyl (-CH=CH-) or methylene (-CH2-), ethylene (-CH2-CH2-) or trimethylene (-CH2-CH2-CH2-). Preferred is the ethylene bridge (-CH2-CH2-) in the case where R1 is phenoxy or phenalkoxy and ethylenyl (-CH=CH-) when R1 is phenyl. The substitutents on the benzene ring in Formula I, R1, R2 and R3, may be hydrogen, halogen (F, C1, Br or I with F or C1 preferred), C1-4 alkyl, C1-4 haloalkyl (halo as above), phenyl, substituted phenyl in which the substi tuent is halogen, C1-4 alkoxy, C2-3 alkanoyloxy, or Cl4 haloalkyl (halo as above), hydroxyl, or substituted hydroxyl in which the sub stitutent is C19 alkyl, C2-8 alkanoyl, benzoyl, phenyl, halophenyl, phenyl-Cl 3-alkyl, cinnamyl, C1 4 haloalkyl, allyl, Cycloalkyl C13 alkyl, adamantyl C13 alkyl, or substituted phenyl C13 alkyl, in which the substituent is halogen (as above), C1-4 alkoxy, C1-4 alkyl or C1 4 haloalkyl (halo as above) The preparation of the compounds of this invention is illustrated in the Flow Sheets. Flow Sheet I shows the general scheme for synthesizing compounds with a vinylene bridge between the lactone and benzene rings. A starting benzaldehyde is converted to the corresponding cinnamaldehyde (this forms the bridging group) and this is subjected to an aldol reaction to elaborate a hydroxy keto ester from the terminal aldehydic moiety. Reduction of the hydroxy keto ester affords the dihydroxy ester which upon lactonization, gives the lactone. The lactone is then separated chromatographicaily into its cis and trans racemates and the latter racemate is resolved to give the desired 4 R trans enantiomer. Flow Sheet II shows the further conversion of the 4 R trans lactones into the corresponding dihydroxy acids and their salts and esters. Although this sequence is shown with the -CH=CH- bridged compounds, the same sequence can be used to give the corresponding acids, salts and esters of any of the other bridged compounds. Flow Sheet III shows the synthetic routes for the preparation of the alternative bridging groups, represented by E in formula I. Compounds with a direct bond between the lactone and phenyl rings are made by the process of Flow Sheet I with omission of step 2. In this instance, the starting benzaldehyde is used directly in the Aldol reaction. Compounds with a methylene (-CH2-) bridge are prepared by starting with the appropriate phenylacetaldehyde in place of the cinnamaldehyde. Compounds with an ethylene (-CH2-CH2-) bridge between the rings are prepared by reduction of the vinylene bridged compounds prepared in Flow Sheet I. Compounds with a trimethylene bridge (-CH2-CH2-CH2-) are prepared by starting with the appropriate l-bromo-3-phenylpropane. Compounds of formula I wherein A is a methyl group are prepared as indicated in Flow Sheet IV. Starting with the appropriate aldehyde, condensation with l-(tri-n-butylstannyl)propane-2-one affords a hydroxy ketone which can be converted to the target lactones either by a) acylation with 2-bromoacetyl bromide followed by intramolecular Reformatsky cyclization or b) acylation with acetyl chloride followed by intermolecular Reformatsky reaction with ethyl 2-bromoacetate followed by lactonization of the dihydroxy ester. Separation of the cis and trans racemic lactones and the subsequent resolution of the trans racemate to obtain the 4 R enantiomer are carried out as described in Flow Sheet I. Flow Sheet V shows the details of the synthesis of benzaldehyde having an ortho phenyl group, followed by their use in the general scheme of Flow Sheet I to form compounds of this invention. This Flow Sheet summarizes the use of the benz aIde- hydes so made in the synthesis of vinylene bridged compounds as in Flow Sheet I, but they obviously can also be used as described in Flow Sheet III to produce compounds with other bridging groups. Because of the extremely high potency of the pyranones having a (6-phenyl)phenyl group, these compounds, pre- pared as in Flow Sheet V, are especially to be pre- ferred. Flow Sheet VI shows an alternate preparation of the 6-phenyl substituted benzaldehydes IIIa. The imines formed between aniline and substituted benzaldehydes are treated with palladium (II) acetate to give stable complexes. These complexes are reacted with substituted phenyl Grignard reagents in the presence of triphenylphosphine to give, after acidic hydrolysis, the 6-phenyl substituted benzaldehydes lIla. FLOW SHEET I Synthesis of Vinylene Bridged Compounds EMI12.1 DEFINITIONS: R1, R2 and R3 are as defined in spec ifications for Formula I. FLOW SHEET II Preparatior. of Salts, Esters, Free Dihydroxy Acids EMI13.1 DEFINITIONS: R1, R2 and R3 are as defined in the specifi- cations for Formula I R5=C1-5 lower alkyl or C1-5 lower alkyl substituted by a phenyl, dimethylamino or acetamino group a pharmaceutically acceptable cation. FLOW SHEET III CQMPOUNDS WITH OTHER BRIDGING GROUPS A. Direct Bond from Phenyl Ring to Lactone Ring [Procedure of Flow Sheet I omitting step (2)]. EMI14.1 B. Methylene Bridge EMI14.2 C. Ethylene Bridge EMI15.1 D. Trimethylene Bridge EMI15.2 Def nitions: R1, R2 and R3 are as defined in the specification for Formula I. FLOW SHEET IV SYNTHESIS of SUBSTITUTED 4-HYDROXY-4-METHYL- 3,4,5,6-TETRAHYDRO-2H-PYRAN-2-ONES EMI16.1 DEFINITIONS: E, R1, R2 and R3 are defined in the speci- fications given for Formula I. FLOW SHEET V SYNTHESIS OF 6-[2-(6-phenylphenyl)ethenyl)pyranones EMI17.1 EMI18.1 Definitions: R1 and R2 are as defined in the speci fication for Formula I. X and Y are halogen, C1-4 alkyl or C1 4alkoxy- III a, IV a, VIII a and X are the special variants here of the general compounds in Flow Sheet I. FLOW SHEET VI ALTERNATES PREPARATION OF BENZALDEHYDES III a EMI19.1 Definitions: R1 and R2 are as defined in the speifi- cation for formula I. X and Y are halogen, C1-4 alkoxy or C1-4 alkyl. REACTIONS IN FLOW SHEETS I-VI 1. When R1, R2 or R3 is HO- or bears a hydroxyl substituent, the HO- group is etherified using a reagent R4X in a suitable solvent such as DMF and the like in the presence of a suitable base, preferably an alkali metal carbonate such as K2CO3, to give the corresponding ether R4O- which can be carried through the remainder of the synthesis. If it is desired to remove R4 at a later synthetic step, R4 is chosen as an easily removable group such as CH3OCH2CH2OCH2- (the MEM protecting group). The MEM group is removed readily by treatment with a Lewis acid catalyst such as ZnBr2 in a suitable solvent such as CH2C12 and the like. When the starting material is devoid of a hydroxyl group, step (1) is omitted. 2. Aldol Reaction. This can be run in several ways: a) The classical Aldol synthesis in which acetaldehyde is condensed with the starting benzaldehyde1 the resulting -hydroxyaldehyde is. acetylated with acetic anhydride and acetic acid is eliminated thermally to give the corresponding cinnamaldehyde. b) The directed Aldol condensation in which the anion of an appropriately N-substituted ethylidenylimine, such as ethylidenecyclohexylimine and the like, is condensed with the starting benzaldehyde at or below room temperature in an aprotic solvent, such as THF and the like, to afford a -hydroxy-ss-phenyl- propylidenylimine which, upon concomitant dehydration and imine hydrolysis in an acidic medium, such as dilute aqueous HC1, provides the corresponding cinnamal- dehyde. c) The use of a nucleophilic acetalde hyde equivalent in which cis-2-ethoxyvinylithium,gen erated from cis- l-ethoxy-2-tri-n-butylstannylethylene, is condensed with the starting benzaldehyde to give an allylic alcohol which is subsequently rearranged, under suitable acidic conditions, to the corresponding cinnamaldehyde. 3) Dianion Step. Reaction with the dianion of acetoacetic ester in a suitable aprotic solvent such as THP', dioxane and the like. 4) Reduction with NaBH4 in a suitable solvent such as methanol, ethanol and the like at or below room temperature. 5) tactonization. Saponification by base (e.g. NaOH) in aqueous alcohol followed by acidification and cyclodehydration by heating in toluene. NOTE: Steps 3, 4 and 5 are usually carried out sequentially without purification of compounds V and VI. 6) Separation of the cis and trans race- mic mixtures by chromatography on silica gel or crystallization. 7) Resolution of the trans racemate into its enantiomers by treating the (-)-trans lactone with either d-(+) or 1-(-)- &alpha; a-methylbenzylamine to give the diastereomeric dihydroxy amides which are separated by chromatography or crystallization. Hydrolysis of each pure diastereomeric amide under basic conditions, such as ethanolic NaOH and the like, affords the corresponding enantiomerically pure dihydroxy acid which, upon lactonization, e.g., in refluxing toluene, provides the pure (+)-trans or (-trans enantiomer. Stereochemistry depends on the absolute stereochemistry of the diastereomeric amide from which it is derived. 8) Saponification with M+OH where is an alkali metal cation. 9) Careful acidification. 10) Mild hydrolysis 11) Nucleophilic opening of the lactone ring with an alcohol, R5OH, in the presence either of a basic catalyst, particularly the corresponding alkoxide, R5O, or an acidic catalyst such as an acidic ion exchange resin, e.g. Amberlite 120. 12) Hydrogenation in the presence of a suitable catalyst such as Rhodium or Palladium on carbon. 13) Reaction with NaCN in a suitable solvent such as aqueous ethanol and the like. 14) Reduction with DIBAH in a aprotic solvent such as toluene, ether, THF and the like followed by work up with an aqueous acid such as 5% H2SO4. - 15) Aldol condensation with l-(tri-n-butyl s,tannyl)propan-2-one. 16) Acylation with 2-bromoacetyl bromide. 17) Intramolecular Reformatsky reaction carried out, for example, in the presence of activated zinc dust, cuprous bromide and diethylaluminum chloride. 18) Acylation with acetyl chloride. 19) Intermolecular Reformatsky reaction carried out with ethyl 2-bromacetate, for example, in the presence of the reagents indicated in step (17) above. 20) Treatment with N-bromosuccinimide in CC14 with irradiation by a sun lamp (Tetrahedron Letters, 3809 (1979)). 21) Treatment with two equivalents of the amine EMI23.1 22) Reaction with SOC12 (.J. Org. Chem., 43, 1372 (1978)). 23) Reaction with a substituted phenyl Grignard reagent EMI23.2 24) Reaction with methyl iodide in a suitable solvent such as acetone. 25) Reaction with NaBH4 in a suitable solvent such as ethanol or methanol. 26) Heating with acid (J. Het. Chem., 3, 531 (1966)). 27) Reaction with Palladium (II) acetate in acetic acid at reflux. 28) Reaction with a substituted Grignard reagent EMI23.3 in suitable solvents such as benzene or toluene in the presence of tri- phenylphosphine. 29) Hydrolysis with 6N HC1 at ambient temperature. A further aspect of the present invention is a pharmaceutical composition consisting of at least one of the compounds of formula I in association with a pharmaceutical vehicle or diluent. The pharmaceutical composition can be formulated in a classical manner utilizing solid or liquid vehicles or diluents and pharmaceutical additives of a type appropriate to the mode of desired administration. The compounds can be administered by an oral route, for example, in the form of tablets, capsules, granules or powders, or they can be administered by a parenteral route in the form of injectable preparations. The dose to be administered depends on the unitary dose, the symptoms, and the age and the body weight of the patient. A dose for adults is preferably between 200 and 2,000 mg per day, which can be administered in a single dose or in the form of individual doses from 1-4 times per day. A typical capsule for oral administration contains active ingredient (250 mg), lactose (75 mg) and magnesium stearate (15 mg). The mixture is passed through a 60 mesh sieve and packed into a No. 1 gelatin capsule. A typical injectible preparation is produced by asceptically placing 250 mg of sterile active ingredient into a vial, asceptically freezedrying and sealing. For use, the contents of the vial are mixed with 2 ml of physiological saline, to produce an injectible preparation. The compounds of this invention also have useful antifungal activities. For example, they may be used to control strains of Penicillium sp., Aspergillus niger, Cladosporium sp., Cochliobolus miyabeorus and Hilminthosporium cynodnotis. For those utilities they are admixed with suitable formulating agents, powders, emulsifying agents or solvents such as aqueous ethanol and sprayed or dusted on the plants to be protected. This invention can be illustrated by the following examples in which ratios of solvents are in volumes and percentages, unless otherwise indicated, or by weight. EXAMPLE 1 Preparation of (E)-6-[2-(2,4-Dichloro-6-phenylmethoxyphenyl)ethenyl]-3,4,5,6-tetrahydro-4-hydroxy2H-pyran-2-one Step A. Preparation of 2,4-Dichloro-6-phenylmethoxy- ben zaldehyde Potassium carbonate (9.4 g, 67.8 mmole) was added to a stirred solutiow of 4,6-dichlorosalicylalde- hyde (10.8 g, 56.5 mmole) in dimethylformamide (80 ml). The resulting mixture was stirred at 60 for 30 minutes and treated with benzyl bromide (10.6 g, 62.1 mmole). This mixture was stirred one hour at 600C and then poured into ice water (I000 ml) to give the title compound (15.9 g, 100%) which melted at 98-1000C after recrystallization from hexane. pmr (CDCl3) # 5.10 (2H, s), 7.33 (5H, s) , 10.40 (H, s). Analysis Calc. for C14H10Cl2O2 Calc.: C, 59.81; H, 3.58 Found: C, 59.98; H, 3.58. Step B. Preparation of (E)-2,4-Dichloro-6 phenylmethoxyc innamaldehyde A stirred suspension of 2,4-dichloro-6phenyl-methoxybenzaldehyde (15.5 g, 55.1 mmole) in acetaldehyde (30 ml) was cooled to 50C and treated with 25% methanolic potassium hydroxide (1.4 ml, 6.24 mmole) at such a rate that the internal tem perature was maintained at 25-300C. The resulting solution was stirred for -30 minutes in the ice bath, treated with acetic anhydride (30 ml) and then heated at 1000C for 30 minutes. After cooling to 300C the solution was treated with water (84 ml) and 12N hydrochloric acid (7 ml). The resulting mixture was refluxed for 30 minutes and then cooled in an ice bath to give a gummy solid which was recrystallized from cyclohexane to give the title compound (5.6 g, 33%), mp 109-1120C: pmr (CDC13) 5.10 (2H, s), 7.33 (5H, s), 9.68 (H, d). Analysis Calc. for C16H12C1202 Calc.: C, 62.56; H, 3.94 Found: C, 62.66; H, 3.98. Alternate Step B. Preparation of (E)-2,4-Dichloro 6 -phenylmethoxycinnamaldehyde A 1.6M solution (18.8 ml, 30 mmole) of nbutyllithium in hexane was added cautiously to a stirred solution of freshly distilled diisopropylamine (3.0 g, 30 mmole) in anhydrous tetrahydrofuran (200 ml) maintained at OOC under a nitrogen atmosphere. The resulting solution was stirred at OOC for 15 minutes and then treated with ethylidenecyclohexylamine (3.75 g, 30 mmole). The solution was stirred 15 minutes at OOC, cooled to -78 C and treated with a solution of 2 ,4-dichloro-6-phenylmethoxybenzaldehyde (8.4 g, 30 mmole) in anhydrous tetrahydrofuran (50 ml). The resulting red solution was stirred at -780C for 15 minutes and then at 250C for 60 minutes. The reaction solution was treated with water (200 ml) and extracted with ether (3 x 200 mull. The organic extracts were combined, washed with brine (3 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the desired intermediate hydroxyimine as a brown viscous oil (12.5 g): pmr (CDC13) g 5.10 (2H, s), 5.50 (H, t), 7.37 (.5H, S), 7.70 (H, s). A solution of the oily imine (12.5 g) in tetrahydrofuran (110 ml) was treated with a solution of oxalic acid dihydrate (11 g, 87.2 mmole) in water (22 ml). The resulting solution was ref fluxed for 30 minutes, cooled to 250C and poured into water (500 ml). The resulting mixture was extracted with ether (3 x 200 ml). The organic extracts were combined, washed with brine (3 x 50 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the title compound as a tan solid. The title compound was purified by recrystallization from cyclohexane to give yellow needles (4.7 g, 51%) melting at 109-111 C: pmr (CDCl3) # # 5.11 (2H, s), 7.33 (5H, s), 9.68 (H, d). Alternate to Alternate Step B. Preparation of (E)2,4-Dichloro-6-phenylmethoxycinnamaldehyde A 1.37 M solution (24.1 ml, 33 mmole) of n-butyllithium in hexane was added cautiously to a stirred solution of cis-l-ethoxy-2-tri-n-butylstannylethylene (11.9 g, 33 mmole) in anhydrous tetrahydrofuran (75 ml) maintained at -780C under a nitrogen atomosphere. The resulting solution was stirred at -780C for one hour and then treated with a solution of 2,4-dichloro-6-phenylmethoxybenzaldehyde (8.4 g, 30 mmole) in anhydrous tetrahydrofuran C50 ml). The resulting brown solution was stirred at -780C for one hour and then allowed to warm to 200C. The reaction solution was quenched with saturated aqueous sodium bicarbonate (25 mlr, diluted with water (100 mlZ and then extracted with ether (2 x 200 ml). The organic extracts were combined, washed with brine (2 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the desired intermediate allylic alcohol as a yellow oil. The oil was chromatographed on a silica column (400 g to cause allylic rearrangement to the desired product. Elution with methylene chloride (200 ml) provided a forerun containing tetrabutyltin which was discarded. Continued elution with methylene chloride/methanol (98:2, V:V; 1500 ml) gave the title compound as a pale yellow solid, mp 109-llloC (6.4 g, 70%). Step C. Preparation of Methyl (E)-7-(2,4-dichloro-6 phenylmethoxyphenyl)-5-hydroxy-3-oxo-6 heptenoate Methyl acetoacetate (9.56 g, 82.3 mmole) was added dropwise to a stirred suspension of sodium hydride (50% oil suspension) (3.95 g, 82.3 mmole) in anhydrous tetrahydrofuran at OOC under a nitrogen atmosphere. The resulting solution was stirred 15 minutes at OOC and then treated with a 1.6M solution (51.5 ml, 82.3 mmole) of n-butyllithium in hexane over 5 minutes. The resulting yellow solution was stirred 15 minutes at OOC and then treated with a solution of (E)-2,4-dichloro-6-phenylmethoxycinnamaldehyde (25.3 g, 82.3 mmole) in anhydrous tetrahydrofuran (150 ml). The resulting orange solution was stirred 15 minutes at OOC and then quenched by dropwise addition of 12N hydrochloric acid (ca. 20 ml). The reaction mixture was diluted with water (100 ml) and extracted with ether (3 x 300 mull. The organic extracts were combined1 washed with brine (3 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the title compound as a yellow oil (34.8 g, 100%): pmr (CDCl3) 6'2.75 (2H, d), 3.45 (2H, s), 3.72 (3H, s), 4.71 (H, m), 5.50 (2H, s), 7.37 (5H, s). Step D. Preparation of Methyl (E)-7-(2,4-Dichloro- 6-phenylmethoxypheny)-3,5-dihydroxy-6 heptenoate Sodium tetrahydridoborate (1.55 g, 41.1 mmole) was added with stirring to a cooled solution (50 C) of methyl (E)-7-(2.4-dichloro-6-phenylmethoxy- phenyl)-5-hydroxy-3-oxo-6-heptenoate (34.8 g, 82.3 mmole) in ethanol (200 ml)' at a rate sufficient to maintain the internal temperature at 15-20 C. The resulting solution was stirred with ice-bath cooling for 15 min. and then acidified with 6N hydrochloric acid. The resulting mixture was diluted with water (500 ml) and extracted with ether (3 x 250 ml). The organic extracts were combined, washed with brine (4 x 100 ml), dries over magnesium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the title compound as a yellow oil (34.8 g, 99.5%) pmr CDCl3) g2.45 (2H, dY) 3.65 (3H, s), 4.18 (H, m) 4.45 (H, m), 4.98 (2H, s). 7.28 (5H, s). Step E. Preparation of (E)-7-(2,4-Dichloro-6-phenyl methoxyphenyl)-3,5-dihydroxy-6-heptenoic acid A solution of methyl (E)-7-(2,4-dichloro-6phenylmethoxyphenyl)-3,5-dihydroxy-6-heptenoate (34.8 g, 81.8 mmole), 1N sodium hydroxide (82 ml, 82 mmole) and ethanol (200 ml) was stirred at 250C for 15 min. The reaction solution was acidified with 6N hydro chloric acid, diluted with water (400 ml) and extracted with ether (3 x 200 mull. The combined organic extracts were washed with brine (3 x 100 ml), dried over magnes ium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the title compound as an orange oil (33.3 g, 99%): pmr (CDC13) & .47 (2H, d), 4.30 (2H,br m), 4.98 (2H, s), 7.30 (5H, s). Step F. Preparation of (E)-6- L2- (2,4-Dichloro-6- phenylmethoxyphenyl)ethenyl]-3,4,5,6 tetrahydro-4-hydroxy- 2H-pyran- 2-one A solution of (E)-7-(2,4-dichlorophenylmethoxyphenyl)-3,5-dihydroxy-6-heptenoic acid (33.3 g, 81.3 mmole) in toluene (300 ml) was heated at reflux in a Dean-Stark apparatus. After 2 hours the Dean Stark apparatus was replaced with a soxhlet containing 3# molecular sieves (100 g). The solution was refluxed for an additional 4 hours and then the toluene was removed in vacuo leaving a yellow oil (31.7 g) which is a mixture of cis and trans isomers of the title compound. The oil was chromatographed on a silica gel column (900 g). Elution with methylene chlorideacetone (9:1, V:V; 4000 ml) provided a forerun which was discarded. Continued elution with the same eluant (500 ml) gave the trans isomer of the title compound as a pale yellow solid (5.8 g). Further elution of the column with the same eluant (3250 ml) gave a tan solid (8.8 g), which is a mixture of the cis and trans isomers of tbe title compound. This cis/trans mixture was chromatographed using a Waters Prep LC500. Separation of this mixture was accomplished by using two prep PAK-500/silica cartridges in series and eluting with methylene chloride-acetone (9:1, V:V). Using the shave recycle technique, the cis (4.7 g) and the trans (3.3 g) isomers of the title compound were obtained. The fractions of the trans isomer, collected from the two chromatographys, were combined and recrystallized from n-butyl chloride to give the trans isomer of the title compound (.7.3 g, 23%), mp 130-131 C: pmr (CDCl3) J2.64 (2H, m), 4.30 (H, m), 5.07 (2H, s), 5.30 (X, m), 7.42 (5H, s). Analysis Calc for C20H18C12O4 Calc.: C, 61.03; H, 4.61 Found: 6, 61.12; H, 4.60. The cis isomer (4.3 g, 13%) of the title compound melted at 130-131.5 C after recrystallization from n-butyl chloride: pmr (CDCl3) #4.30 (H, m), 4.83 (H, m) , 5.12 (2H, s), 7.47 (5H, s) Analysis Calc. for C20H18C12O4 Calc.: C, 61.08; H, 4.61 Found: C, 61.55; H, 4.63 EXAMPLE 2 Starting with 4,6-dichlorosalicylaIdehyd but substituting equimolar amounts of the following alkyl halide or tosylate in place of benzyl bromide in Step A of Example 1 and following the procedure of Steps A through F there was obtained a corresponding amount of the appropriate end product listed below. Alkyl Halide M.P. or Tosylate End Product Isomer C Calc. Fd. n-pentyl iodide (E)-6-[2-(2,4-dichloro-6- trans 81-83 C 57.92 57.68 n-pentyloxyphenyl)ethenyl]- H 5.94 6.01 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one cis oil C 57.92 57.54 H 5.94 6.09 3,4-dichlorobenzyl (E)-6-#2-[2,4-dichloro- trans 73-75 C 52.27 52.03 chloride 6-(3,4-dichlorophenyl- H 3.73 3.74 methoxy)phenyl]ethenyl# 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one diphenylmethyl (E)-6-[2-(2,4-dichloro-6- trans 135.5- C 66.53 66.71 bromide diphenylmethoxyphenyl) 137 H 4.72 4.63 ethenyl]-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran 2-one allyl bromide (E)-6-[2-(2,4-dichloro-6- trans 82-84 C 55.99 56.14 allyloxyphenyl)ethenyl]- H 4.70 4.70 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one 2-methoxyethoxy- (E)-6-#2-[2,4-dichloro-6- trans oil C 52.19 52.04 methyl chloride (2-methoxyethoxymethoxy)- H 5.15 5.05 phenyl]ethenyl# -3,4,5,6 tetrahydro-4-hydroxy-2H pyran-2-one <RTI ID=33.1> Alkyl Halide M.P. or Tosylate End Product Isomer C Calc. Fd. methylthio- (E)-6-[2-(2,4-dichloro-6- trans oil C 49.60 49.97 methyl chlor- methylthiomethoxyphenyl)- H 4.44 4.74 ide ethenyl]-3,4,5,6-tetrahydro 4-hydroxy-2H-pyran-2-one 2-(adamant-1- (E)-6-#2-[2,4-dichloro-6 trans 174-6 C 64.52 64.38 yl)ethyl tol- (2-adamant-1-ylethoxy)- H 6.52 6.70 uene-p-sul- phenyl)ethenyl# -3,4,5,6phonate tetrahydro-4-hydroxy-2H pyran-2-one 4-chlorobenzyl (E)-6-#2-[6-(4-chloro- trans 111.5- C 56.16 56.08 bromide phenylmethoxy-2,4- 113 H 4.01 3.98 dichlorophenyl]-ethenyl# 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one 1-bromo-3- (E)-6-#2-[2,4-dichloro-6- trans 93-94 C 62.71 62.66 phenylpro- (3-phenylpropoxy)phenyl]- H 5.26 5.25 pane ethenyl# -3,4,5,6-tetrahy dro-4-hydroxy-2H-pyran 2-one 1-bromo-2- (E)-6-[2-(2,4-dichloro-6- trans 125- C 61.93 62.18 phenyl- phenethylphenyl)ethenyl]- 126 H 4.95 5.07 ethane 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one cinnamyl (E) (E)-6-#2-[2,4-dichloro- trans 120- C 63.02 62.73 bromide 6-(3-phenyl-2-propenyloxy)- 122 H 4.81 4.81 phenyl]ethenyl# -3,4,5,6 tetrahydro-4-hydroxy-2H pyran-2-one Alkyl Halide M.P. or Tosylate End Product Isomer C Calc. Fd. 1-bromo-3,5,5- (E)-6- 2-[2,4-dichloro-6- trans 53-66 C 61.54 61.60 trimethyl- (3,5,5-trimethylhexyl- H 7.04 7.27 hexane oxy)phenyl]ethenyl-3, 4,5,6-tetrahydro-4 hydroxy-2H-pyran-2 one 4-methylbenzyl (E)-6- 2-[2,4-dichloro- trans 113- C 61.92 62.05 bromide 6-(4-methylphenyl- 118 H 4.95 5.05 methoxy)phenyl]ethenyl 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one 4-methoxybenzyl (E)-6- 2-[2,4-Dichloro- trans 114- C 59.58 59.73 bromide 6-(4-methoxyphenyl 115 H 4.76 4.75 methoxy)phenyl]ethenyl 3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one 4-fluorobenzyl (E)-6- 2-[2,4-dichloro- trans 124-6 C 58.41 58.26 bromide 6-(4-fluorophenyl- H 4.17 4.20 methoxy)phenyl]ethenyl 3,4,5,6-tetrahydro-4- cis C 58.41 58.29 hydroxy-2H-pyran-2-one 131-3 H 4.17 4.06 2-fluorobenzyl (E)-6- 2-[2,4-dichloro- trans 140- C 58.41 58.43 chloride 6-(2-fluorophenylmethoxy)- 141.5 H 4.17 4.20 phenyl]ethenyl -3,4,5,6 tetrahydro-4-hydroxy-2H pyran-2-one Alkyl Halide M.P. or Tosylate End Product Isomer C Calc. Fd. 2,4-difluorobenzyl (E)-6-#2-[2,4-dichloro-6- trans 138- C 55.96 56.00 bromide (2,4-difluorophenylmethoxy) 139 H 3.76 3.82 phenyl]ethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran-2 one 3-fluorobenzyl (E)-6-#2-[2,4-dichloro-6(3- trans 91.5- C 58.41 58.48 bromide fluorophenylmethoxy)- 92 H 4.17 4.22 phenyl]ethenyl#-3,4,5,6 tetrahydro-4-hydroxy-2H pyran-2-one 2,3,4,5,6-penta- (E)-6-#2-[2,4-dichloro-6- trans 72- C 49.90 49.87 fluorobenzyl- (2,3,4,5,6-pentafluoro- 75 H 3.33 3.35 chloride phenylmethoxy)phenyl] ethenyl#-3,4,5,6-tetrahydro -4-hydroxy-2H-pyran-2-one. 0.5 chlorobutane EXAMPLE 3 By substituting an equimolar amount of 3, 5-dichlorosalicylaldehyde for 4,6-dichlorosalicylaldehyde and an equimolar amount of n-.pentyl iodide for benzyl bromide in Step A of Example 1 and following the procedure for Steps A through F, there was obtained a corresponding amount of the following end product. (E)-6-[2-(3,5-Dichloro-2-pentyloxyphenyl)ethenyla- 3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one, trans isomer: yellow oil. Analysis calc for C18H22Cl204 Calc.: C, 57.92; H, 5.94 Found: C, 57.83; H, 5.91 cis isomer: yellow oil. Analysis calc. for C18H22C1204 Calc;: C; 57.92; H, 5.94 Found: C, 57.54; H, 6.09. EXAMPLE 4 By substituting an equimolar amount of the following aldehydes in place of 2,4-dichloro-6-phenylmethoxybenzaldehyde in Step B of Example 1 and then following the procedures of Steps B through F, there was obtained a corresponding amount of the appropriate end product listed below. M.P. Aldehyde End Product Isomer C Calc. Fd. 2,4-dichloro- (E)-6-[2-(2,4-dichloro- trans 146- C 54.37 54.57 benzaldehyde phenyl)ethenyl]-3,4,5,6- 148 H 4.21 4.31 tetrahydro-4-hydroxy-2H pyran-2-one cis 115- C 54.37 54.24 117 H 4.21 3.96 2,4-dimethyl- (E)-6-[2-(2,4-dimethyl- trans 111- C 73.14 73.07 benzaldehyde phenyl)ethenyl]-3,4,5,6- 113 H 7.36 7.46 tetrahydro-4-hydroxy-2H pyran-2-one cis 110- C 73.14 72.77 112 H 7.36 7.57 2,6-dichloro- (E)-6-[2-(2,6-dichloro- trans 102- C 54.37 54.65 benzaldehyde phenyl)ethenyl]-3,4,5,6- 104 H 4.21 4.21 tetrahydro-4-hydroxy-2H pyran-2-one cis 118- C 54.37 54.37 119 H 4.21 4.17 2-chloro- (E)-6-[2-(2-chlorophenyl)- trans 129 C 61.79 61.89 benzaldehyde ethenyl]-3,4,5,6-tetra- 131 H 5.18 5.38 hydro-4-hydroxy-2H-pyran 2-one 4-phenyl- (E)-6-[2-(4-biphenyl)- trans 148- C 77.53 77.74 benzaldehyde ethenyl]-3,4,5,6- 150 H 6.16 6.02 tetrahydro-4-hydroxy 2H-pyran-2-one EXAMPLE 5 By substitutina an equimolar amount of the following aldehydes for (E) -2, 4-dichloro-6-phenylmethoxy- cinnamaldehyde in Step C of Example 1 and following the procedure for Steps C through F, there was obtained a corresponding amount of the appropriate end product listed below. M.P. Aldehyde End Product Isomer C Calc. Fd. benzaldehyde 3,4,5,6-tetrahydro-4- trans 91- C 68.73 68.56 hydroxy-6-phenyl-2H 93 H 6.29 6.49 pyran-2-one benzaldehyde 3,4,5,6-tetrahydro-4- cis 91- C 68.73 68.72 hydroxy-6-phenyl-2H- 93 H 6.29 6.41 pyran-2-one (E)-cinnamaldehyde (E)-3,4,5,6-tetrahydro-4- trans 96- C 71.54 71.41 hydroxy-6-(2-phenylethenyl)- 98.5 H 6.46 6.59 2H-pyran-2-one (E)-cinnamaldehyde (E)-3,4,5,6-tetrahydro-4- cis 91- C 71.54 71.79 hydroxy-6-(2-phenylethenyl)- 92.5 H 6.46 6.53 2H-pyran-2-one 4-phenylbenz- 6-(4-biphenyl)-3,4,5,6- trans 135- C 76.10 76.28 aldehyde tetrahydro-4-hydroxy- 137 H 6.01 5.68 2H-pyran-2-one 4-phenylbenz- 6-(4-biphenyl)-3,4,5,6- cis 146- C 76.10 76.11 aldehyde tetrahydro-4-hydroxy- 148 H 6.01 5.67 2H-pyran-2-one 2,4-dichloro- 6-(2,4-dichlorophenyl)- trans 133- C 50.60 50.88 benzaldehyde 3,4,5,6-tetrahydro-4- 136 H 3.86 3.87 hydroxy-2H-pyran-2-one 2,4-dichloro- 6-(2,4-dichlorophenoxy- trans "oil" C 49.51 49.24 phenoxyacet- methyl)-3,4,5,6-tetrahydro- H 4.15 4.10 aldehyde 4-hydroxy-2H-pyran-2-one EXAMPLE 6 Preparation of (E) -6- [2- (2 ,3-Dichlorophenyl) ethenyll - 3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2 one Step A. preparation of (E)-2,3-Dichlorocinnamaldehyde Anhydrous aluminum chloride (10.5 g, 78.8 mmole) was slowly added to a stirred solution of 2,3-dichlorobenzoyl chloride (14.4 g, 68.7 mmole) and bis-trimethylsilylacetylene (12.8 g, 75.1 mmole) in dry methylene chloride maintained at 0 C. The dark brown reaction mixture was stirred 5 minutes at 0 C and 2 hours at 250C and then poured into ice water. The organic product was extracted into ether (4 x 200 ml). The ether extracts were combined, washed with brine (3 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the desired intermediate trimethylsilylacetylenic ketone as a brown oil (18.4 g, 98%), pmr (CDC13) 60,30 (9H, s), 7.47 (3H, m). Sodium methoxide (0.81 g, 15.0 mmole) was added to a stirred solution of the oily trimethylsilylacetylenic ketone (18.4 g, 67.8 mmole) in methanol (200 ml) maintained at OOC in an ice bath. After 5 minutes the ice bath was removed and the reaction solution was stirred 30 minutes at 25 C. The resulting solution was cooled to OOC and sodium tetrahydridoborate (0.9 g, 23.8 mmole) was slowly added. This reaction solution was stirred 30 minutes at 250C and then poured into ice water. The organic product was extracted into ether (4 x 150 ml). The ether extracts were combined, washed with brine (3 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo, leaving the desired 5-hydroxydimethylacetal as a brown oil. Hydrochloric acid (6N, 50 ml) was added to the solution of the crude 8-hydroxydimethylacetal in dioxane (100 ml). The resulting mixture was heated on the steam bath for 30 minutes, and then poured into ice water (1000 ml) to give the title compound as a brown solid (12.7 g). This solid was chromatographed on a silica gel column (700 g). Elution with ethyl acetate-hexane (2:8, V:V; 1250 ml) provided a forerun which was discarded. Continued elution with the same eluant (1125 ml) gave the title compound (10.6 g, 78%) which melted at 94-950C: pmr (CDC13)6.70 (H, dd), 7.98 (H, d), 9.86 (H, d). Analysis Calc. for CgH6C12O Calc.: C, 53.76; H; 3.01 Found: C, 53.52; H, 2.86 Step B. Preparation of (E)-6-[2-(2,3-Dichlorophenyl) ethenyl] -3,4,5, 6-tetrahydro-4-hydroxy-2H- pyran- 2-one By substituting an equimolar amount of (E)-2,3-dichlorocinnamaldehyde for (E) -2, 4-dichloro- 6-phenylmethoxycinnamaldehyde in Step C of Example 1 and following the procedure for Steps C through F, there was obtained a corresponding amount of the title compound. trans isomer: mp 122-1230C. Analysis Calc. for C13H12Cl2O3 Calc.: C, 54.37; H, 4.21 Found: C, 54.31; H, 4.25. cis isomer: mp 13l-1320C. Analysis Calc. for C13H12Cl2O3 Calc.: C, 54.37; H, 4.21 Found: C, 54.52; H, 4.25. EXAMPLE 7 Preparation of (E)-6-[2-(2-Biphenyl)ethenyl]-3,4,5,6 tetrahydro-4-hydroxy-2H-pyran-2-one By substituting an equimolar amount of 2-biphenylcarbonyl chloride for 2,3-dichlorobenzoyl chloride in Step A of Example 6 and following the procedure for Steps A and B, there was obtained a corresponding amount of the title compound. trans isomer: mp 89-91 C. Analysis Calc. for C19H18O3 Calc.: C,- 77.53; H, 6.16 Found: C, 77.51; H, 6.17. cis isomer: yellow glass Analysis Calc. for C19H18O3 Calc.: C, 77.53; H, 6.16 Found: C, 77.26; H, 6.07. EXAMPLE 8 Preparation of Trans- 6- [2- (2 , 4-Dichloro-6-phenyl- methoxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy2H-pyran-2-one A solution of trans-(E)-6-[2-(2,4-dichloro- 6-phenylmethoxyphenyl)ethenyl]-3,4,5,6-tetrahydro-4hydroxy-2H-pyran-2-one (1.1 g, 28 mmole) in tetrahydrofuran (50 ml) was magnetically stirred and hydrogenated at room temperature and atmospheric pressure in the presence of 110 mg of 5% rhodium on carbon catalyst until 1.5 molar equivalents of hydrogen had been consumed. After removing the catalyst by filtration, the filtrate was evaporated in vacuo leaving the title compound as a pale yellow oil. The oil was chromatographed on a silica gel column (200 g). Elution with acetone-methylene chloride (1:9, V:V; 560 ml) provided a forerun which was discarded. Continued elution with the same eluant (240 ml) gave the title compound as a colorless oil which solidified when it was triturated with ether. The title compound was purified by recrystallization from ether-hexane (1:1, V:V; 20 ml) to give colorless needles (0.67 g, 61%) melting at 99-1010C: pmr (CDC13)g1.83 (4H, m) , 2.60 (2H, m) , 2.90 (2H, m) '4.30 (H, m) , 4.62 (H, m) , 5.05 (2H, s) , 7.42 (5H, s) Analysis Calc. for C20H20C1204 Calc.: C, 60.77; H, 5.10 Found: C, 60.96; H, 4.85. EXAMPLE 9 By substituting an equimolar amount of the following 2H-pyran-2-ones for trans-(E)-6-[2-(2, 4- dichloro-6-phenylmethoxyphenyl)ethenyl]-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran-2-one in Example 8 and following the procedure of Example 8, there was obtained a corresponding amount of the following end products. M.P. 2H-Pyran-2-one End Product C. Calc. Fd. trans-(E)-6-(2-phenyl- trans-6-(2-phenylethyl)-3,4- 76-77 C 70.88 71.02 ethenyl)-3,4,5,6- 5,6-tetrahydro-4-hydroxy-2H- H 7.32 7.41 tetrahydro-4-hydroxy pyran-2-one trans-(E)-6-[2-(2,4- trans-6-[2-2,4-dichlorophenyl)- 95-97 C 54.00 53.80 dichlorophenyl)- ethyl]-3,4,5,6-tetrahydro-4- H 4.88 4.71 ethenyl]-3,4,5,6- hydroxy-2H-pyran-2-one tetrahydro-4-hydroxy cis-(E)-6-[2-(2,4- cis-6-[2-(2,4-dichlorophenyl)- oil C 54.00 53.89 dichlorophenyl)- ethyl]-3,4,5,6-tetrahydro-4- H 4.88 4.90 ethenyl]-3,4,5,6- hydroxy-2H-pyran-2-one tetrahydro-4-hydroxy trans-(E)-6-[2-(2- trans-6-[2-(2-biphenyl)ethyl]- oil C 77.00 77.17 biphenyl)ethenyl]- 3,4,5,6-tetrahydro-4-hydroxy- H 6.80 6.85 3,4,5,6-tetrahydro- 2H-pyran-2-one 4-hydroxy M.P. 2H-Pyran-2-one End Product C. Calc. Fd. cis-(E)-6-[2-(2,4- cis-6-[2-(2,4-dichloro-6- 96-98 C 60.77 60.73 dichloro-6-phenyl- phenylmethoxyphenyl)ethyl]- H 5.10 5.24 methoxyphenyl)ethenyl]- 3,4,5,6-tetrahydro-4-hydroxy3,4,5,6-tetrahydro-4- 2H-pyran-2-one hydroxy trans-(E)-6-[2-(2,4- trans-6-[2-(2,4-dichloro- 154- C 59.85 59.87 dichloro-6-cyclohexyl- 6-cyclohexylmethoxyphenyl)- 155 H 6.53 6.44 methoxyphenyl)ethenyl]- ethyl]-3,4,5,6-tetrahydro3,4,5,6-tetrahydro-4- 4-hydroxy-2H-pyran-2-one hydroxy trans-(E)-6-[2-(2,4- trans-6-[2-(2,4-dichloro- oil C 59.85 59.87 dichloro-6-phenoxy- 6-phenoxyphenyl)ethyl]- H 4.76 5.10 phenyl)ethenyl]- 3,4,5,6-tetrahydro-43,4,5,6-tetrahydro- hydroxy-2H-pyran-2-one 4-hydroxy M.P. 2H-Pyran-2-one End Product C. Calc. Fd. trans-(E)-6-#2-[2,4- trans-6-#2-[2,4-dichloro 151- C 58.12 58.13 dichloro-6-(4-fluoro- 6-(4-fluorophenylmethoxy)- 152 H 4.63 4.68 phenylmethoxy)phenyl]- phenyl]ethyl#-3,4,5,6-tetraethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyranhydro-4-hydroxy 2-one trans-(E)-6-#2-[2,4- trans-6-#2-[2,4-dichloro 98- C 58.12 58.24 dichloro-6-(2-fluoro- 6-(2-fluorophenylmethoxy)- 100 H 4.63 4.63 phenylmethoxy)phenyl]- phenyl]ethyl#-3,4,5,6-tetraethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyranhydro-4-hydroxy 2-one trans-(E)-6-#2-[2,4- trans-6-#2-[2,4-dichloro- 106- C 58.12 58.20 dichloro-6-(3-fluoro- 6-(3-fluorophenylmethoxy)- 115 H 4.63 4.72 phenylmethoxy)phenyl]- phenyl]ethyl#-3,4,5,6-tetraethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyranhydro-4-hydroxy 2-one EXAMPLE 10 Preparation of (E) -6- [2-(3,4-Dichlorophenyl) ethenyl]- <RTI ID=49.3> 3,4,5,6-tetrahydro-4-hydPoxy-2fP-pyran2 Step A. Preparation of (E)-3,4-Dichlorocinnamaldehyde The reaction mixture containing sodium tetrahydrodiborate (0.76 g, 20 mmole), cadmium chloride . 2.5 dimethylformamide (3.7 g, 12.6 mmole) and hexamethylphosphoramide (5 ml) in acetonitrile (100 ml) was stirred magnetically at OOC for 5 minutes. A solution of (E)-3-phenyl-2-propenoyl chloride (4.7 g, 20 mmole) in acetonitrile (25 ml) was rapidly added to the stirred reaction mixture and stirring was continued for 5 minutes. The reaction mixture was quenched with 6N- hydrochloric acid and poured into water (500 ml). This aqueous mixture was extracted with ether (3 x 200 ml). The ether extracts were combined, washed with brine (3 x 100 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the title compound as a yellow solid. This yellow. solid was chromatographed on a silica gel column (200 g). Elution with methylene chloride (500 ml) provided a forerun which was discarded. Continued elution with the same eluXlt (550 ml) gave the title compound as a light yellow solid (2.2 g, 54%), melting at 92-940C: pmr (CDCl3) #6.6 (H, dd), 9.72 (H, d). Step B. Preparation of (E)-6-12- (3, 4-Dichlorophenyl) - ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one By substituting an equimolar amount of (E)-3,4-dichlorocinnamaldehyde for (E)-2,4-dichloro6-phenylmethoxycinnamaldehyde in Step C of Example 1 and following the procedure for Steps C through F, there was obtained a corresponding amount of the title compound. trans isomer: mp 116-118 C. Analysis Calc. for C13H12C12O3 Calc.: C, 54.37; H, 4.21 Found: C, 54.60; H, 3.96. EXAMPLE 11 Preparation of Trans-(E)-6-[2-(2,4-dichloro-6-phenoxy Phenyl)ethnyl]=3,4,5,6-tetrahydro-4-hydroxy-2Hpyran- 2-one Step A. Preparation of 2,4-Dichioro-6-phenoxy- benzaldehyde Sodium methoxide (0.54 g, 10 mmole) was added to a stirred solution containing 4,6-dichlorosalicylaldehyde (1.9 g, 10 mmole) in methanol (15 ml). After stirring the reaction solution for 15 minutes 250C, diphenyliodonium chloride (3.16 g, 10 mmole) was added in one portion. The resulting reaction mixture was refluxed for 30 hours and then concentrated in vacuo. The residue was suspended in water (100 ml) and the mixture was extracted with ether (3 x 50 ml, The ether extracts were combined, washed with brine (2 x 50 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the title compound as a brown oil which solidified upon trituration with hexane. This solid was recrystallized from hexane to give pale yellow needles (0.8 g, 30%) which melted at 99-1010C, pmr (CDCl3) # 6.68 (H, d), 7.28 t6H, m) , 10.58 (H, s) Analysis Calc. for C13H8C1202 Calc.: C, 58.45; H, 3.02 Found: C, 58.26; H, 3.01. Step B. reparation of Trans-(E)-6-[2-(2,4-dichloro-6- phenoxyphenyl¯ethenyl]-3,4,5,6-tetrahydro 4 -hydroxy- 2H-pyran- 2-one By substituting an equimolar amount of 2,4-dichlorp-6-phenoxybenzaldehyde in place of 2,4 dichloro-6-phenylmethoxybenzaldehyde in Step B of Example 1 and following the procedure for Steps B through F, there was obtained a corresponding amount of the title compound: trans isomer, mp l24-1260C. Analysis Calc. for C19H16C1204 Calc.: C, 60.17; H, 4.25 Found: C, 60.33; H, 4.30. EXAMPLE 12 By Substituting an equimolar amount of the following substituted diphenyliodonium chlorides for diphenyliodonium chloride in Step A of Example 11 and following essentially the procedure of Steps A and B in Example 11, there was obtained a corresponding amount of the following end product. Substituents on End Product Diphenyl iodonium chloride 4,4'-difluoro trans-(E) -6-C2-[2,4-dichloro 7w--fluorophenoxy) phenyl] - ethenyl}-3,4,5,6-tetrahydro-4- hydroxy-2H-pyran-2-one 2,2'-methoxy trans-(E)-6-{2-[2,4-dichloro 6-(Z-methoxyphenoxy)phenyl]- ethenyl}-3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one 4,4'-dimethyl trans-(E)-6-{2-[2,4-dichloro 6-(4-methylphenoxy)phenyl] ethenyl}-3,4,5,6-tetrahydro-4 hydroxy-2H-yran-2-one EXAMPLE 13 Resolution of the Optical Isomers of (-)Trans-(E)- 6-[2-(2,4-dichlorophenyl)ethenyll-3,4,5,6-tetrahydro- 4-hydroxy-2H-pyran-2-one Step A. Preparation and Separation of Diastereomeric Amides (Diastereomers A and B) + A solution of (+)-trans-(E)-6-[2-(2,4- dichlorophenyl) ethenyl] -3,4,5, 6-tetrahydro-4-hydroxy- 2H-pyran-2-one (2.87 g, 10 mmole) in d-(+)-a-methylbenzylamine (15 ml) was stirred for 18 hours at 250C and then poured into water (100 ml). This aqueous mixture was acidified with 6N hydrochloric acid and then extracted with ether (3 x 100 ml). The ether extracts were combined, washed with brine (4 x 75 ml), dried over magnesium sulfate and filtered. Evapora- tion of the filtrate in vacuo gave the intermediate diastereomeric amides as a tan viscous oil (4.1 g). The tan viscous oil (3.1 g, 7.6 mmole) was chromatographed on a silica gel column (200 g). Elution with acetone-methylene chloride (1:4, V:V; 1200 ml) gave a forerun which was discarded. Continued elution with the same eluant (1000 ml) gave the diastereomeric amides as a viscous oil (3.0 g). The diastereomeric amides were separated by chromatography on a Waters Prep LC500. The separation was accomplished by using two prep PAR-500 silica cartridges in series and eluting with acetonemethylene chloride (1:4, V:V). Using the shaverecycle technique, diastereomer A (1.36 g) and diastereomer B (1.20 g) were obtained. Recrystallization of diastereomer A from n-butyl chloride gave colorless clusters (1.0 g) which melted at 106-1080C: pmr (CDCl3) #1.47 (3H, d), 2.33 (2H, d), 4.30 (H, m), 5.17 (H, q), 7.33 (8H, m). Analysis Calc. for C21H23Cl2NO3 Calc.: C, 61.77; H, 5.68; N, 3.43 Found: C, 61.78; H, 5.78; N, 3.50. Recrystallization of diastereomer B from n-butyl chloride-petroleum ether gave a pale yellow solid which melted at 55-600C: pmr (CDC13) g 1.47 (3H, d), 2.33 (2H, d) , 4.30 (H, m) , 5.17 (H, q) , 7.33 (8H, m). Analysis Calc. for C21H23C12NO3 Calc.: C, 61.77; H, 5.68; N, 3.43 Found: C, 61.41; H,d5.87; N, 3.30. Step B. Preparation of (+)-Trans-(E)-6- [2-(2,4 dichlorophenyl)ethenyl]-3,4,5,6-tetrahydro- 4-hydroxy-2H-pyran-2-one Diastereomer A (0.74 g, 1.8 mmole) of Step A was dissolved in 95% ethanol (25 ml) containing 1N sodium hydroxide (3.6 ml, 3.6 mmole) and the solution was refluxed for 54 hours. The solvent was removed in vacuo and the residue was suspended in water (100 ml) and acidified with 6N hydrochloric acid. This aqueous mixture was extracted with ether (3 x 75 ml). The ether extracts were combined, washed with brine (2 x 50 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the intermediate acid as a yellow oil (0.54 g). A solution of the yellow oil in toluene (150 ml) was refluxed through a soxhlet containing molecular sieves (3#) for 5 hours. The solution was evaporated in vacuo leaving the title compound as a yellow solid. The title compound was purified by recrystallization from ether and then n-butyl chloride to give white needles (0.11 g, 20%) melting at 114-1150C, pmr (CDC13) g 2.03 (2H, m), 2.73 (2H, m), 4.46 (H, m), 5.41 (H, m), 6.19 (H, ddv 7.01 (H, d), 7.14-7.50 (3H m). Analysis Calc. for C13H12C1203 Calc.: C, 54.37; H, 4.21 Found: C, 54.51; H, 4.32. [&alpha;]D25 = +5.9 (c 0.425; chloroform) Step C. Preparation of (-)Trans-(E)-6-[2-(2,4- Dichlorophenyl)ethenyl]-3,4,5,6-tetrahydro 4-hydroxy-2H-pyran-2-one Diastereomer B (1.1 g, 2.7 mmole) of Step A was dissolved in 95% ethanol (25 ml) containing 1N sodium hydroxide (5.4 ml, 5.4 mmole) and the solution was refluxed for 18 hours. The ethanol was removed in vacuo and the residue was suspended in water (100 ml) and acidified with 6N hydrochloric acid This aqueous mixture was extracted with ether (2 x 100 ml). The ether extracts were combined, washed with brine (3 x 50 ml), dried over magnesium sulfate and filtered. The filtrate was evaporated in vacuo leaving the intermediate acid as a yellow oil (0.85 g). A solution of the yellow oil in toluene (150 ml) was refluxed through a soxhlet containing molecular sieves (3A) for 5 hours. The solution was evaporated in vacuo leaving the title compound as a yellow solid. The title compound was recrystallized twice from n-butyl chloride to give white needles (0.34 g, 44%) melting at 114-1150C: pmr (CDCl3)#2.03 (2H, m), 2.73 (2H, m), 4.46 (H, m), 5.41 (H, m), 6.19 (H, dd), 7.01 (H, d), 7.147.50 (3H, m). Analysis Calc. for C13H12C1203 Calc.: C, 54.37; H, 4.21 Found: C, 54.31; H, 4.26. 25 [a] = -6.6 (c 0.555; chloroform) EXAMPLE 14 -Resolution of the Optical Isomers of (t) Trans-6-[22,4-dichloro-6-phenylmethoxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one Step A. Preparation and Separation of Diastereomeric Amides (Diastereomers A and B) By substituting an equimolar amount of (t) trans6-[2-(2,4-dichloro-6-phenylmethoxyphenyl)ethyl]-3,4,5,6tetrahydro-4-hydroxy-2H-pyran-2-one for (t) trans- (E) -6 [2- (2, 4-dichlorophenyl) ethenyl] -3,4,5, 6-tetrahydro-4-hy- droxy-2H-pyran-2-one and replacing d- (+) -a-methylbenzyl- amine with the l-(-)-isomer in Step A of Example 13 and following the procedure described in Step A of Example 13, there was obtained a corresponding amount of the diastereomeric amides: Diastereomer A: mp 177-179 C; pmr (CDCl3) #1.45 (3H, d), 2.22 (2H, d), 2.83 (2H, m), 3.74 (H, m), 4.13 (H, m), 5.04 (2H, s), 6.86 (H, d), 7.05 (H,d), 7.33 (5H, s), 7.82 (5H,s) Analysis Calc. for C28H31C12N04 Calc.: C, 65.11; H, 6.05; N, 2.71 Found: C, 65.28; H, 6.34; N, 2.95 Diastereomer B: mp 130-1320C; pmr (CDCl3) #1.45 (3H,d), 2.22 (2H,d), 2.83 (2H, m), 3.74 (H, m), 4.13 (H, m), 5.04 (2H, s), 6.86 (H,d), 7.05 (H, d), 7.33 (5H, s), 7.82 (SH, s). Analysis Calc. for C28H31C12NO4 Calc. : C, 65.11; H, 6.05; N, 2.71 Found: C, 65.24; H, 6.27; N, 2.88 Step B. Preparation of (+)-Trans-6-[2-(2,4-dichloro- 6-phenylmethoxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy2H-pyran-2-one .0.1 n-Butyl Chloride Solvate By substituting an equimolar amount of diastereomer A from the preceeding step for diastereomer A in Step B of Example 13 and following the procedure described therein, there was obtained a corresponding amount of the title compound, mp 108-1120C; pmr (CDC13) 1.91 (4H, m), 2.61 (2H, m), 2.93 (2H, m), 4.30 (H, m), 4.70 (H, m), 5.06 (2H, s), 6.33 (H, d), 7.02 (H, d), 7.43 (5H, s). Analysis Calc. for C20H20Cl2O4.0.1C4H9Cl Calc.: C, 60.56; H, 5.21 Found: C, 60.93; H, 5.73 [&alpha;]D25 = + 16.6 (c, 0.1; chloroform) Step C. Preparation of (-)-Trans-6-[2-(2,4-dichloro-6- phenylmethoxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy 2H-pyran-2-one.0.1 n-But'yl Chloride Solvate By substituting an equimolar amount of diastereomer B from Step A above for diastereomer B in Step C of Example 13 and following the procedure described therein, there was obtained a corresponding amount of the title compound, mp 104-1110C; pmr (CDCl3) #1.91 (4H, m), 2.61 (2H, m), 2.93 (2H,m), 4.30 (H, m), 4.70 (H, m), 5.06 (2H, s), 6.33 (H, d) 7.02 (H, d), 7.43 (5H, s). analysis Calc. for C20H20Cl2O4.0.1C4H9Cl Calc.: C, 60.56; H, 5.21 Found: C, 60.62; H, 5.46 25 = -17.7 (c, 0.1; chloroform) EXAMPLE 15 Preparation of (E)-6-[2-(2,6-Dichlorophenyl)ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-4-methyl2H-pyran-2-one Step A. Preparation of 6-(2,4-Dichlorophenyl)-4 hydroxy-5-hexene-2-one 2-Acetoxypropene (3.3 ml, 30 mmole) and trin-butyltin methoxide (5.7 g, 24 mmole) were combined and stirred at 60-700C under N2 for 1 hour then placed under vacuum for an additional 30 minutes. 3-(2,4-Dichlorophenyl)propenal (4g, 20 =ole) was added and the reaction mixture was stirred at 700C under N2 for 4 hours. The clear reaction mixture was then cooled, treated with malonic acid (1 g, 10 =ole) in ether (20 ml) and refluxed for 30 minutes. After cooling to -200C, the reaction mixture was filtered and the precipitate was washed with ether (4 x 10 ml). The ethereal solutions were combined, evaporated and the residual oil was chromatographed on a 60 mm column with 15 cm of silica gel (230-400 mesh). Elution with chloroform-methanol (99:1, V:V; 2.0 L) provided the title compound as a thick yellow oil (4.2 g, 81%); pmr CDC13) 2.2 (3H, s), 2.73 (2H, d), 4.73 (H, m), 6.10 (H, dd). Analysis Calc. for C12Hl2C1202 Calc.: C, 55.62; H, 4.67 Found: C, 55.55; H, 4.72 Step B. Preparation of 6-(2,4-Dichlorophenyl)-2-oxo 5-hexene-4-yl 2-Bromoacetate 2-Bromoacetyl bromide (1.1 ml, 13.2 mmole) was added dropwise to a stirred solution of 6-(2,4-dichloro phenyl)-4-hydroxy-5-hexene-2-one (3.4 g, 13.1 mmole) and pyridine (1.07 ml, 13.2 mmole) in ether (100 ml) at OOC. The ice bath was removed and the reaction mixture was stirred at 200C for 2 hours and then diluted with R2O (100 ml). The organic layer was separated and washed with 1N HC1 (100 ml), R2O (2 x 100 ml) and brine, dried over MgSO4, filtered and evaporated. The residual oil was chromatographed on a 60 mm column with 15 cm of silica gel (230-400 mesh) Elution with methylene chloride-acetone (99:1; V:V; 1.9 L) provided the title compound (2.8 g, 56%); pmr (CDC13) 2.2 (3H, s), 2.92 (2H, t), 3.85 (2H, s), 5.9 (H, m), 6.15 (H, m) 6.95-7.5 (4H, m). Step C. Preparation of (E)-6-[2-(2,5-Dichlorophenyl) ethenyl] -3,4,5, 6-tetrahydro-4-hydroxy-4-methyl- 2H-pyran-2-one A solution of 6-(2,4-dichloropheny1)-2-oxo-5- hexene-4-yl 2-bromoacetate (2.8 g, 7.4 mmole) in dry TRF (50 ml) was added dropwise to a vigorously stirred slurry of activated zinc dust (720 mg, 11.1 mmole), cuprous bromide (60 mg, 0.4 mmole), diethylaluminum chloride (25% solution in toluene; 3.2 ml, 8 mmole) and dry TRF (50 ml) under N2 at 200 C. Stirring was continued for 5 hours before quenching with pyridine (8 ml) followed by addition of H2 0 (500 ml) and ether extraction (3 x 150 ml). The combined ether extracts were washed with 1N HCL (2 x 50 ml), R2O (2 x 250 ml) and brine, then dried (MgSO4), filtered and evaporated in vacuo leaving a sticky, pale yellow solid (1.8 g) which is a mixture of cis and trans isomers of the title compound. This crude product was digested once with ether (40 ml) and then crystallized from n-butyl chloride (25 ml) to provide the trans isomer of the title compound as tiny, colorless crystals (550 mg), mp 136-1380C. Analysis Calc. for C14Hl4C1203 Calc.: C, 55.83; H, 4.69 Found: C, 56.07; H, 4.66 The filtrates from digestion and crystallization vida supra were combined, evaporated and chromatographed using a Waters Prep Lie500. The separation was accomplished by using two prep PAK 500/silica cartridges in series and eluting with methylene chloride-acetone (15:1, V:V). By using the shave-recycle technique, the cis (220 mg) and the trans (230 mg) isomers of the title compound were separated. The cis isomer of the title compound was crystallized from n-butyl chloride-hexane (2:1, V:V) to give 120 mg of solid, mp 135-1370C. Analysis Calc. for C14Hl4C1203 Calc.: C, 55.83; H, 4.69 Found: C, 55.46; H, 4.71. The epimeric alcohols are readily distinguished by analytical TLC (fluorescent silica gel (40 A), 1 x 3 in, MK6F, Whatman) and elution with methylene chloride-acetone (9:1; V:V): cis alcohol, Rf 0.25; trans alcohol, Rf 0.30. EXAMPLE 16 Alternate Route to (E)-6-12-(2, 6-Dichlorophenyl) ethenyl]- 3,4,5, 6-tetrahydro-4-hydroxy-4-methyl-2H-pyran-2-one Step A. Preparation of 6-(2,4-Dichlorophenyl)-2-oxo 5-hexene-4-yl Acetate Acetyl chloride (1.2 ml, 16.5 mmole) was added dropwise to a stirred solution of 6-(2,4-dichlorophenyl)-4hydroxy-5-hexene-2-one (3.9 g, 15 =ole) in pyridine (60 ml) at OOC. The ice bath was removed, and the reaction mixture was stirred at 200C for 2 hours and then diluted with ether (300 ml). The ether solution was washed with 1N HC1 (3 x 300 ml) and saturated NaHCO3, dried over MgSO4, filtered and evaporated. The residual pale amber oil (4.1 g) was chromatographed on a 50 mm column with 15 cm of silica gel (230-400 mesh). Elution with methylene chloride (2 L) provided the title compound as a pale yellow oil (3.95 g, 87%): pmr (CDC13) 2.03 (3H, s), 2.17 (3H, s), 2.83 (2H, dd). Analysis Calc. for C14H14C1203 Calc.: C, 55.83; H, 4.69 Found: C, 55.82; R, 4.76 Step B. Preparation of Ethyl 5-Acetoxy-7-(2,4-dichloro phenyl)-3-hydroxy-3-methyl-6-heptenoate A solution of 6-(2,4-dichlorophenyl)-2-oxo-5- hexene-4-yl acetate (1.3 g, 4.3 mmole) and ethyl bromoacetate (0.47 ml, 4.2 mmole) in dry THF (10 ml) was added dropwise to a vigorously stirred slurry of activated zinc dust (490 mg, 7.5 mmole), cuprous bromide (29 mg, 0.2 mmole), diethylaluminum chloride (25% solution in toluene; 1.72 ml, 4.3 =ole) and dry THF (5 ml) under N2 at 200C. Stirring was continued for 5 hours before quenching with pyridine (3.5 ml). After the addition of water (50 ml) the mixture was extracted with ether (3 x 80 ml). The combined ether extracts were washed with 1N HC1 (2 x 25 ml), R2O (2 x 50 ml) and brine, then dried (MgS04), filtered and evaporated leaving the crude title compound as a pale yellow oil (1.2 g); pmr (CDC13)t 1.28 (3H, t), 1.33 (3H, t), 2.10 (3H, s). Step C. Preparation of (E)-6-[2-(2,6-Dichlorophenyl)- ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-4-methyl 2H-pyran-2-one Ethyl 5-acetoxy-7-(2,4-dichlorophenyl)-3-hydroxy3-methyl-6-heptenoate (1.2 g, 3.2 mmole) was stirred with 1N NaOH (6.4 ml, 6.4 =ole) at 500C. for 1 hour. The aqueous solution was diluted with H2O (50 ml) and washed with ether (2 x 50 ml). The aqueous layer was acidified with 12N HC1 and extracted with ether (2 x 50 ml). The combined organic extracts were washed with brine, dried over MgSO4, filtered and evaporated leaving the crude diol acid which was lactonized by refluxing in toluene (75 ml) under a Soxhlet extractor filled with 3A sieves for 3 hours. The toluene was evaporated and the residue was chromatographed on a 50 mm'column with 15 cm of silica gel (230-400 mesh). Elution with chloroform-methanol (19:1; V:V; 400 ml) provided (120 mg, 12%) of the title compound as a mixture of cis (46%) and trans (54%) isomers as determined by RPLC. EXAMPLE 17 Preparation of (E)-6¯[2-(2' -methaxy-l,l' -biphenyl-2- yl)ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran- 2-one. Step A. Preparation of 2- (2' -Methoxy-l,l' -biphenyl-2- yl)-4,4-dimethyl-2-oxazoline. 2-Methoxyphenylmagnesium bromide, prepared from 2-bromoanisole (22.4 g, 120 mmol) and magnesium (2.9 g, 120 mmol), in dry THF (75 ml) was added dropwise to a stirred solution of 2-(2-methoxyphenyl)-4,4-dimethyl2-oxazoline (20.4 g, 100 mmol) in dry THF (150 ml) under N2 at 200C. Stirring of the solution was continued for 20 hours and then the reaction mixture was quenched by the addition of saturated ammonium chloride solution. The resulting mixture was extracted with ether (2 x 500 ml), dried over MgSO4, filtered and evaporated. The residue was chromatographed on silica gel (ethyl acetate-hexane) to provide title compound as colorless crystals (25.3 g, 90%), mp 129-1310C. Step B. Preparation of 2'-Methoxy-l,l'-biphenyl-2carboxylic acid. 2-(2'-Methoxy-1,1'-biphenyl-2-yl)-4,4-dimethyl2-oxazoline (.25 g, 90 mmol) was dissolved in 4.5N HC1 (1.5L) and heated at reflux for 20 hours. After cooling, the heterogeneous mixture was extracted with ether (3 x 200 ml). The etheral extracts were combined and washed with R2O and brine, dried over MgSO4, filtered and then evaporated to provide the title compound (15.4 g, 75%), mp 196-1970C, as a colorless solid. Step C. Preparation of 2'-Methoxy-l,l'-biphenyl-2- carbonyl Chloride. 2'-Methoxy-1,1'-biphenyl-2-carboxylic acid (32 g., 100 mmole) was dissolved in thionyl chloride (40 ml) and the solution was refluxed for 3 hours. The solution was evaporated to provide the title compound. Step D. Preparation of (E)-6-[2-(2'-Methoxy-l,l"-biphenyl- 2-yl)ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H- pyran-2-one. Starting with 2'-methoxy-1,1'-biphenyl-2-carbonyl chloride in place of 2,3-dichlorobenzoyl chloride, the title compound was prepared following the procedures of Examples 6, Step A, and then Example 1, Steps C through F. EXAMPLE 18 Preparation of (E)-6-12-(4'-chloro-1,1'-biphenyl-2-yl)- ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one Starting with 4-chlorophenylmagnesium bromide, in place of the 2-methoxyphenylmagnesium bromide, the title compound was prepared following the procedure of Example 17, Steps A through D. EXAMPLE 19 Preparation of (E)-6-12-(4'-Fluoro-l,l'-biphenyl-2-yl)- ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one Starting with 4-fluorophenylmagnesium bromide, in place of the 2-methoxyphenylmagnesium bromide, the title compound was prepared following the procedure of Example 17, Steps A through D. EXAMPLE 20 Preparation of (E)-6-[2-(4'-methyl-1,1'-biphenyl-2-yl)ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. Starting with 4-methylphenylmagnesium bromide, in place of the 2-methoxyphenylmagnesium bromide, the title compound was prepared following the procedure of Example 17, Steps A through D. EXAMPLE 21 Preparation of 6- [2- (2, 4-Dichloro-6-hydroxyphenyl) ethyl] = 3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one Step A. Preparation of 6-2-[2,4-Dichloro-6-(2-methoxy- ethoxymethoxy) phenyl] ethyl}-3,4,5, 6-tetrahydro-4 hydroxy-2H-pyran-2 -one . Starting with (E)-6- 2- [2, 4-Dichloro-6- (2-methoxy ethoxymethoxy) phenyl] ethenyl -3,4,5, 6-tetrahydro-4-hydroxy- 2H-pyran-2-one and following the procedure of Example 8, the title compound was obtained as a viscous golden oil: pmr (CDCl3) 2.63(2H, d), 3.36 (3H, s), 3.55 (2H, m), 3.8 (2H m), 4.35 (H, m), 4.73 (H, m), 5.27 (2H, 5), 7.6 (2H, dd). Step B. Preparation of 6-{2-(2,4-Dichloro-6-hydroxyphenyl}- ethyl5-3,4,5, 6-tetrahydro-4-hydroxy-2H-pyran-2-one Zinc bromide (2.4 g, 10 itirnole) was added to a solution of 6-±2- [2, 4-dichloro-6- (2-methoxyethoxymethoxy) phenyl]ethyl}-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one (780 mg, 2 mmole) in methylene chloride (12 ml). The resulting mixture was stirred at 200C for 2 hours, then quenched with saturated sodium bicarbonate solution (50 ml) and diluted with ether (200 ml). The ethereal layer was washed with brine, dried over MgSO4, filtered and evaporated. The residue was chromatographed on a 50 = low-pressure column with 6 inches of silica gel (230-400 mesh). The column was eluted with 470 ml of methylene chloride-acetone (4:1, V:V). The next 330 ml provided the title compound as a golden glass (100 mg., 16%): pmr (CDCl3) #2.62 (2H, d), 4.29 (H, m), 4.78 (H, m), 6.87 (2H, s). EXAMPLE 22 Preparation of 6- 2-[2,4-Dichloro-6- (4-trifluoromethylphenylmethoxy)phenyl]ethyl -3,4,5,6-tetrahydro-4-hydroxy2H-pyran-2-one. By following the procedure of Example 1, step A, but substituting equimolar amounts of 6- 2-(2,4-dichloro 6-hydroxyphenyl) ethyl -3,4,5,6-tetrahydro-4-hydroxy-2H- pyran-2-one and 4-trifluoromethylbenzyl bromide for the 4,6-dichlorosalicylaldehyde and benzyl bromide used therein, the title compound is obtained, m.p. 104-1050C. EXAMPLE 23 Preparation of 6-[2-(2-acetoxy-4,6-dichlorophenyl)ethyl]- 3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. Acetyl chloride (0.08 ml, 1.05 mmole) was added dropwise to a stirred solution of 6-[2-(2,4-dichloro-6- hydroxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran2-one (300 mg, 1 mmole) and pyridine (0.09 ml, 1.05 mmole) in ether (10 ml) at OOC. The ice bath was removed and the reaction mixture was stirred at 200C for 1 hour and then diluted with R2O (10 ml). The organic layer was separated and washed with lN HC1 (10 ml), R2O (2 x 10 ml) and brine, dried over MgSO4, filtered and evaporated to provide the title compound. EXAMPLE 24 Preparation of 6-[2- (2-Benzoyloxy-4, 6-dichlorophenyl) ethyl]- 3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. By following the procedure of Example 23, but substituting an equivalent amount of benzoyl chloride for the acetyl chloride used therein, the title compound is obtained. EXAMPLE 25 Preparation of (E) -Trans-6- [2- (3-trifluoromethylphenyl) ethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. By substituting an equimolar amount of 3-trifluoromethylbenzaldehyde for 2, 4-dichloro-6-p.henylmethoxy- benzaldehyde in step B of Example 1 and following Steps B through F, there is obtained a corresponding amount of the title compound. EXAMPLE 26 Preparation of Trans-6- (2-chlorophenylmethyl) -3,4,5,6- tetrahydro-4-hydroxy-2H-pyran-2-one By substituting an equimolar amount of o-chlorophenylacetaldehyde for 2, 4-dichloro-6-phenylmethoxybenzal- dehyde in step B of Example 1 and following Steps B through F there is obtained a corresponding amount of the title compound. EXAMPLE 27 Preparation of (E)-6-[2-(2,4-dichloro-6-methoxyphenylethenyl]-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. The procedure of Example 1 is followed using an equivalent amount of methyl iodide in place of benzyl bromide in Step A. The compound named above is obtained. EXAMPLE 28 Preparation of (E)-6- [2- (2, 4-dichloro-6-cyclopropylmethoxy- phenyl) ethenyl] -3, 4, 5, 6-tetrahydro-4-hydroxy-2H-pyran-2-one. The procedure of Example 1 is followed using an equivalent amount of cyclopropylmethyl iodide in place of benzyl bromide in Step A. The above named compound is obtained. Example 29 Trans-6- (3-phenylpropyl) -3,4,5, 6-tetrahydro-4-hydroxy- 2H-pyran-2-one. Step A. Preparation of 4-Phenylbutyronitrile A mixture of l-bromo-4-phenylbutane (58.8 g, 0.24 mole) and sodium cyanide (25 g, 0.5 mole) in ethanol (300 ml)-water (100 ml) is heated at reflux with stirring for 5 hours. The resulting reaction mixture is concentrated in vacuo and extracted with ether. The ethereal extract is filtered and evaporated at reduced pressure to afford the title compound which is purified by distillation. Step B. Preparation of 4-Phenyl-l-butanal To a stirred suspension of 4-phenylbutyronitrile (21.7 g, 0.12 mole) in ether (400 ml) at 780C is added 85 ml. of 25.3% diisobutylaluminum hydride in toluene over a period of 1 hour. After an additional i hour, the dry ice-acetone bath is removed and the reaction mixture is stirred at ambient temperature for 3 hours. The reaction mixture is added slowly to 5% aqueous sulfuric acid and then is extracted with several portions of ether. The ether extracts are combined, washed with water and saturated aqueous sodium chloride solution and dried over magnesium sulfate. After removal of the ether, the residual yellow oil is distilled in vacuo to give an oil. Step C. Trans-6-(3-phenylpropyl)-3,4,5,6-tetrahydro-4 hydroxy-2H-pyran-2-one. This product is prepared from 4-phenyl-1-butanal in a manner similar to Steps C, D and E in Example 1. The product is purified by column chromatography and high pressure liquid chromatography to give the title compound. Example 30 Preparation of 6-{3-[6-[(4-acetoxyphenyl)methoxy]-2,4 dichlorophenyl] ethyl -3,4,5, 6-tetrahydro-4-hydroxy-2H pyran-2-one. By following the procedure of Example 1, Step A, but substituting equimolar amounts of 6-[2-(2,4-dichloro6-hydroxyphenyl)ethyl]-3,4,5,6-tetrahydro-4-hydroxy-2Hpyran-2-one and 4- (bromomethyl) phenol acetate for the 4,6-dichlorosalicylaldehyde and benzyl bromide used therein, the title compound is obtained. Example 31 Preparation of 6-{2-[2,4-Dichloro-6-(4-hydroxyphenylmethoxy phenyl] ethyl -3,4,5, 6-tetrahydro-4-hydroxy-2H-pyran-2-one. By following the procedure of Example 16, Step C, but substituting an equimolar amount of 6-[ 2-[6-(4-acetoxy- phenylmethoxy)-2,4-dichlorophenyl]ethyl-3,4,5,6-tetrahydro-4-hydro4-hydroxy-2H-pyran-2-one for the ethyl 5-acetoxy-7-(2,4-di- chlorophenyl)-3-hydroxy-3-metyhyl-6-heptenoate used therein, the title compound is obtained. EXAMPLE 32 Preparation of (E) -trans-6-f'2 [3, 5-dichloro-4 '-fluoro- -2-(1,1'-biphenyl)yl]ethenyl}-3,4,5,6-tetrahydro-4 hydroxy- 2H- pyran- 2 -one . Step A. Preparation of 2,4-dichloro-6-methoxyben z aldehyde By substituting an equimolar amount of methyl iodide for benzyl bromide in Step A of Example 1 there was obtained a corresponding amount of the title compound as a white powder, mp 1100-1110C. Step B. Preparation of N-(2-Hydroxy-l,l-dimethyi- ethyl)-2,4-dichloro-6-methoxybenzamide A suspension of 2,4-dichloro-6-methoxybenzaldehyde (3 g, 15 mmol) and N-bromosuccinimide (3.6 g, 20 mmol) in carbon tetrachloride (30 ml) was illuminated with a 150 W flood lamp under nitrogen with vigorous stirring on a steam bath for seven minutes. The cloudy mixture was cooled to 0 C, diluted with methylene chloride (30 ml) and treated dropwise with a solution of 2-amino-2-methylpropanol (3 ml, 30 mmol) in methylene chloride (30 ml). The ice bath was removed and the mixture was stirred at 200 C for twenty hours. The reaction mixture was filtered and the collected solids were washed with additional methylene chloride (50 ml). The clear filtrates were combined and washed with H20 (100 ml), 5% HC1 (100 ml), 5% NaOH (100 ml), H20 (100 ml) and brine, then dried (MgSO4), filtered and evaporated in vacuo to provide the title compound as a white powder (3.6 g, 82%), mp 130-132 C. Crystallization from hexane-toluene (10:8, v:v) provided an analytical sample of title compound, mp 131-132 C. Analysis Calc. for C12H15C12N03 Calc: C, 49.33 H, 5.18 N, 4.79 Found: C, 49.51 H, 5.27 N, 4.62 Step C. Preparation of 2-(2,4-Dichloro-6-methoxy phenyl)-4,4-dimethyl-2-oxazoline N-(2-Hydroxy-1,1-dimethylethyl)-2,4-dichloro 6-methoxybenzamide (5.5 g, 18.8 mmol) was treated dropwise with thionyl chloride (5.5 ml) and stirred magnetically at 200 C for 30 min. Dry ether (100 ml) was added, the mixture was stirred for an additional one hour and the oxazoline hydrochloride precipitate was collected by filtration. The salt was neutralized with 20% sodium hydroxide to afford an alkaline mixture which was extracted with ether. The ethereal extract was dried (MgSO4) and concentrated to give an oil (3.6 g, 70%), which crystallized on standing, mp 47 500 C. Analysis for C12H13C12NO2 Calc: C, 52.57 H, 4.78 N, 5.11 Found: C, 52.60 H, 4.98 N, 4.99 Step D. Preparation of 2- (3, 5-Dichloro-4' -fluoro-2- [1,1'-biphenyl]yl)-4,4-dimethyl-2-oxazoline By substituting equimolar amounts of 2-(2,4 dichloro-6-methoxyphenyl)-4,4-dimethyl-2-oxazoline and 4-fluorophenylmagnesium bromide for 2-(2-methoxyphenyl)- 4,4-dimethyl-2-oxazoline and 2-methoxyphenylmagnesium bromide, the title compound was prepared following the procedure of Example 17, Step A, (85%), mp 93-95 C. Analysis for C17Hl4Cl2FNO Calc: C, 60.37 H, 4.17 N, 4.14 Found: C, 60.72 H, 4.17 N, 3.89 Step E. Preparation of 2-(3,5-Dichloro-4'-fluoro-2 [1,1'-biphenyl]yl)-3,4,4,-trimethyl-2 oxazolium iodide A solution of 2-(3,5-dichloro-4'-fluoro-2 [l,l'-biphenyl]yl)-4,4-dimethyl-2-oxazoline (4.6 g, 13.6 mmol) and methyl iodide (7 ml) in nitromethane (30 ml) was stirred on a steam bath for sixteen hours. The cooled reaction mixture was diluted with dry ether (200 ml) and, after cooling in -an ice-bath, the crystalline product was collected to give 6 g (92%) of the title compound, mp 214-216 C dec. Crystallization from acetonitrile-ether (1:3, v:v) provided an analytical sample of the title compound, mp 218-219.50C dec. Analysis for C18H17C12F1N0 Calc: C, 45.03 H, 3.57 N, 2.92 Found: C, 44.94 H, 3.47 N, 2.83 Step F. Preparation of 3,5-Dichloro-4'-fluoro-l,l'- biphenyl-2-carboxaldehyde A vigorously stirred suspension of 2-(3,5dichloro-4'-fluoro-2-[1,1'-biphenyl]yl-3,4,4-trimethyl2-oxazolium iodide (5.9 g, 12.3 mmol) in ethanol (50 ml) was treated portionwise with sodium borohydride (550 mg, 18 mmol). After stirring for two hours at ambient temperature the clear solution was diluted with 3N hydro chloric acid (100 ml) and stirred on a steam bath for two hours. The reaction mixture was then cooled, diluted with H20 (200 ml) and extracted with ether (300 mlL The ether extract was washed with H20 (2 x 200 ml) and brine, then dried (MgSO4), filtered and evaporated in vacuo to provide 2.72 g (82%) of the title compound, mp 66-68 C. Crystallization from petroleum ether provided an analytical sample of the title compound, mp 73-740 C. Analysis for C13H7C12FO Calc: C, 58.02 H, 2.62 Found: C, 58.15 H, 2.52 Step G. Preparation of (E)-trans-6-[2-(3,5-dichloro- 4'-fluoro-2-[1,1'-biphenyl]yl)ethyl]-3,4,5,6 tetrahydro-4-hydroxy-2H-pyran-2-one By substituting an equimolar amount of 3,5- dichloro-4'-fluoro-1,1'-biphenyl-2-carboxaldehyde in place of 2,4-dichloro-6-phenylmethoxybenzaldehyde in the alternate to alternate Step B, of Example 1 and then following the procedures of Step B through , there was obtained a corresponding amount of the title compound, mp 121-1220 C. Analysis for ClgHl5C12F03 Calc: C, 59.86 H, 3.97 Found: C, 59.70 H, 3.97 The cis isomer of the title compound was obtained in comparable yield after crystallization from n-butyl chloride, mp 107-108 C. EXAMPLE 33 Starting with 2-(2,4-Dichloro-6-methoxyphenyl)4,4-dimethyl-2-oxazoline but substituting equimolar amounts of the following Grignard reagents in place of 4-fluorophenylmagnesium bromide in Step D of Example 31 and following the procedures of Steps D through G there was obtained a corresponding amount of the appropriate end product listed below. Grignard mp Reagent End Product C Calc. Found phenyl (E)-trans-6-[2-(3,5-di- 113-115 C 62.83 C 62.47 chloro-2-[1,1'-biphenyl]- H 4.44 H 4.64 yl)ethenyl]-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran 2-one 4-chlorophenyl (E)-trans-6-[2-(3,4',5- 116.5-118 C 57.38 C 57.07 trichloro-2-[1,1'-biphenyl]- H 3.80 H 3.85 yl)ethenyl]-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran 2-one EXAMPLE 34 Alternate Preparation of 3,5-Dichloro-4'-fluoro-1,1'- biphenyl-2-carboxaldehyde Step A. Preparation of Bis [@-(Acetato-0,0')bis- (3,5-dichloro-2[(phenylimino)methyl]phenyl C,N] dipalladium A mixture of N- [(2, 4-Dichlorophenyl) methylene] benzeneamine (2.5 g, 10 mmole) and palladium (II) acetate (2.24 g, 10 mmole) in acetic acid (50 ml) was heated at reflux for one hour with stirring. The turbid solution was filtered and the filtrate was diluted with water (300 ml) to give the title compound as a red solid (3.9 g, 94%). Crystallization from acetic acid-water (7:1, v:v) provided an analytical sample of the title compound, mp 203-205 C: pmr (CDC13) 1.73 (3H,S), 6.50 (H, d, J=1.5Hz) 6.97 (2He, m), 7.12 (H,d, J=1.5HZ) 7.33 (3H, m), 8.03 (H, S). Analysis Calc. for C30H22C12N404Pd2 Calc: C, 43.42 H, 2.67 N, 3.38 -. Found: C, 43.54 H, 2.59 N, 3.13 Step B. Preparation of 3,5-Dichloro-4'-fluoro-1,1'- biphenyl-2-carboxyldehyde A solution of bis-[CL--(Acetato-O: O')bis- '[3,5- dichloro-2-[(phenylimino)methyl]phenyl-C,N]dipalladium (8.29 g, 10 mmole) and triphenylphosphine (21.0 g, 80 mmole) in dry benzene (150 ml) was stirred for 30 minutes at ambient temperature under N2. The 4-fluoro phenylmagnesium bromide, prepared from 4-bromofluorobenzene (15.4 g, 88 mmole) and magnesium (1.94 g,8Q mmole) in dry ether (100 ml) under N2 at ambient temperature, was added to the above solution in one portion. The resulting mixture was stirred for one hour at ambient temperature. After the addition of 6N HC1 (50 ml) with stirring for one hour, the mixture was filtered. The filtrate was diluted with ether (300 ml) and washed with brine (2 x 100 ml). The organic layer was refiltered to remove more yellow solid and the filtrate, washed with brine (2 x 100 ml), dried over MgSO4, filtered and evaporated. The residue was chromatographed on a silica column (1000 g). Elution with ether-hexane (1:39, v:v, 5500 ml) provided a forerun which was discarded. Continued elution with ether-hexane (1:9, v:v, 5700 ml) gave the title compound as a yellow solid (4.5 g, 84%), mp 73-740 C: pmr (CDC13) m 7.03-7.40 (SH, m), 7.53 (H, d, J-1,5Hz), 10.13 (H,S). EXAMPLE 35 Starting with bis[ -(Acetato-0,0')bis-[3,5dichloro-2[(phenylimino)methyl]phenyl-C,N]dipalladium but substituting equal amounts of the following Grignard reagents for 4-fluorophenylmagnesium bromide in Step B of Example 34 and following the procedure of Step B there was obtained a corresponding amount of the appropriate end product listed below. Grignard End Product pmr (#) 4-methylphenyl 3,5-dichloro-4'-methyl-1,1'- 2.40 (3H, S), 7.13-7.40 biphenyl-2-carboxaldehyde (5H, m), 7.50 (H, d, J=1.5Hz), 10,06 (H, S) 4-methoxyphenyl 3,5-dichloro-4'-methoxy-1,1'- 3.85 (3H, S), 6.93-7.50 biphenyl-2-carboxaldehyde (6H, m), 10.03 (H, S) 3-fluorophenyl 3,5-dichloro-3'-fluoro-1,1'- 6.93-7.60 (6H, m), biphenyl-2-carboxaldehyde 10.16 (H, S) EXAMPLE 36 By substituting an equimolar amount of the following aldehydes for 2,4-dichloro-6-phenyl- methoxybenzaldehyde in alternate to alternate Step B, Example 1, and then following Steps C through F of Example 1, the corresponding pyran-2H-ones listed below were obtained. Analysis Starting Aldehyde End Product mp Calcd Found 3,5-dichloro-4'-methyl- (E)-trans-6-#2-(3,5- 119 - C: 63.67 63.69 1,1'-biphenyl-2-carboxal- dichloro-4'-methyl-2- 119.5 H: 4.81 4.88 dehyde [1,1'-biphenyl]yl) ethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran 2-one 3,5-dichloro-4'-methoxy- (E)-trans-6-#2-(3,51,1'-biphenyl-2-carboxal- dichloro-4'-methoxy-2dehyde [1,1'-biphenyl]yl) ethenyl#-3,4,5,6-tetra hydro-4-hydroxy-2H-pyran -2-one 3,5-dichloro-3'-fluoro- (E)-trans-6-#2-(3,5- 130 - C: 59.86 59.75 1,1'-biphenyl-2-carboxal- dichloro-3'-fluoro-2- 132 H: 3.97 3.94 dehyde [1,1'-biphenyl]yl) ethenyl# -3,4,5,6-tetra hydro-4-hydroxy-2H-pyran 2-one EXAMPLE 37 Resolution of the Optical Isomers of (t) Trans-6 {2-[2,4-dichloro-6-(4-fluorophenylmethoxy)pheny]ethyl}3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one. Step A. Preparation and Separation of the Diastere omeric Amides (Diastereomers A and B) A solution of (#)-trans-6-{2-[2,4-dichloro 6-(4-fluorophenylmethoxy)phenyl]ethyl}-3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2-one (28.3 g, 68 mmole) and 1-(-)- a -methylbenzylamine (16.5 g, 136 mmole) in tetrahydrofuran (350 ml) was refluxed for 20 hours. The tetrahydrofuran was removed in vacuo and the residue was stirred in ether (500 ml) and the precipitate collected to give diastereomer A which was twice stirred for 15 min. in refluxing ether (500 ml) to yield a colorless solid (13.0 g, 36%) which melted at 1850-1880C. Step B. Preparation of (+) Trans-6-±2- [2, 4-dichloro- 6-(4-fluorophenylmethoxy)phenyl]ethyl}-3,4,5,6 tetrahydro-4-hydroxy-2H-pyran-2-one By substituting an equimolar amount of diastereomer A from Step A above for diastereomer A in Step B of Example 13 and following the procedures described therein, there was obtained a corresponding amount of the title compound which was recrystallized from n-butylchloride-pet ether (,4:3, v:v), mp 133 -135 C.; pmr (CDCl3) SI.53-2.20 (5H, m); 2.66 (2H, m), 2.93 (2H, m), 4.36 (H, m), 4.73 (H, m), 5.04 (2H, 5), 6.85 (H, d), 7.03-7.53 (5H, m). Analysis Calc. for C20H19Cl2FO4 Calc: C, 58.12; H, 4.63 Found: C, 58.25; H, 4.71 [&alpha;]D25 + 17.8 (C, 1.0; chloroform) EXAMPLE 38 Preparation of (+)-(E)-(3R*, 5S*)-7-(3,5-dichloro-4' fluoro[1,1'-biphenyl]-2yl)-3,5-dihydroxy-6-heptenoic acid, ammonium salt. Step A. Preparation and Separation of Diastereomeric Amides By substituting an equimolar amount of (#) trans-(E)-6-{2-[3,5-dichloro-4'-fluoro(1,1'-biphenyl] 2-yl)etheny1 -3,4,5,6-tetrahydro-4-hydroxy-2H-pyran-2 one for (#)-trans-6-{2-[2,4-dichloro-6-(4-fluorophenyl- methoxy) phenyl] ethylj -3,4,5, 6-tetrahydro-4-hydroxy-2H- pyran-2-one in Step A of Example 37 and following the procedure described therein there was obtained a corresponding amount of Diastereomer A as colorless crystals which melted at 128.50 - 1290 C. Step B. preparation of (+)-(E)-(3R*, 5S*)-7-{3,5-dichloro 41 -fluoro [1,1' -biphenyl] -2-ylj -3, 5-dihydroxy- 6-heptenoic acid, ammonium salt Diastereomer A (6.2 g, 12.3 mmole) of Step A was dissolved in 95% ethanol (600 ml) containing 1N NaOH (60 ml, 60 mmole) and the solution was refluxed for 16 hours. The solvent was removed in vacuo and the residue was suspended in ice water (200 ml) and ether (500 ml) and subsequently acidified with 3N HC1 (50 ml). The ether layer was washed successively with ice-cold 1N HC1 (200 ml), brine (2 x 200 ml), dried over MgSO4, and filtered. Anhydrous ammonia was bubbled through the cold etherial solution for 2 min. Vigorous stir- ring was then continued at 200C for 1 hour and then the mixture was cooled slowly to ca 50 C. Filtration provided the title compound as tiny colorless needles (4.3 g, 84%), mp 1050-1080 C dec. pmr (d6- DMSO) # 1.15 (H, m), 1.41 (H, m), 1.99 (H, dd), 2.14 (H, dd), 3.66 (H, m), 4.11 (H, dd), 5.52 (H, dd) 6. 38 (H, d), 7.23-7.42 (5H, m), 7.69 (H, d) . Analysis for C19H20C12FNO4 Calc: C, 54.82 H, 4.84 N, 3.36 Found: C, 55.13 H, 4.98 N, 3.07 [c4]27 D

Claims: WHAT IS CLAIMED IS: 1. A process for preparing compounds having the structure wherein EMI86.1 E is a direct bond, -CH2- , -CH2-CH2-, -CH2-CH2-CH2- or -CH=CH- R1, R2 and R3 are each selected from H, halogen, C1-4 alkyl, C1-4 haloalkyl, phenyl, phenyl substituted by halogen, C1-4 alkoxy, C2-8 alkanoyloxy, C14alkyl, or OR4 in which R4 is H, C2-8 alkanoyl, benzoyl, phenyl, halophenyl, phenyl C1-3 alkyl, C1-9 alkyl, cinnamyl, C1-4 haloalkyl, allyl, cycloalkyl-C1-3 alkyl, adamantyl C13 alkyl, or substituted phenyl C13 alkyl in each of which the substituents are selec ted from halogen, C14 alkoxy, C14 alkyl, or C1-4 haloalkyl; and the corresponding dihydroxy acids in which the lactone ring is hydrolytically opened, and the pharmaceutically acceptable salts of said dihydroxy acids, and the lower alkyl and the phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the 4-R enantiomer of the trans racemate whose structure is shown above, which comprises the aldol condensation of the dianion of acetoacetic ester with an aldehyde of the structure EMI87.1 followed by reduction of the 3-keto group to a hydroxyl, saponification of the ester, lactonization of the carboxyl with the 6-hydroxyl, if desired, reducing any double bonds in the bridging group E, and separating the cis and trans racemates and separation therefrom of the 4 (R) trans enantiomers. 2. The process of claim 1 for the preparation of the compound wherein: E is -CH2-CH2- or -CH=CEI-; R1 is halogen in the 2-position; R2 is halogen in the 4-position; R3 is halophenyl-Cl 3 alkoxy, halophenoxy or halophenyl in the 6-position. 3. The process of claim 2 for the preparation of the compound wherein: R1 and R2 are chloro. 4. The process of claim 3 for the preparation of the compound wherein: E is -CH=CH-, and R3 is 4-f luorophenyl. 5. Compounds having the structure EMI89.1 wherein A is H or methyl; E is a direct bond, -CR2- , -CH2-CH2-, -CH2-CH2-CH2- or -CH=CH-; R1, R2 and R3 are ench selected from H, halogen, C1-4 alkyl, C1-4 haloalkyl, phenyl, phenyl substituted by halogen, alkoxy, C2-8 alkanoyloxy, C1-4 alkyl, or OR4 in which R4 is H, C2-8 alkanoyl, benzoyl, phenyl, halophenyl, phenyl C13 @ alkyl, C1-9 alkyl, cinnamyl, C1-4 haloakyl, allyl, cycloalkyl-C1-3 alkyl, adamantyl C1-3 alkyl, or substituted phenyl C1-3 alkyl in each of which the substituents are selec ted from halogen, C1-4 alkoxy, C14 alkyl, or C14 haloalkyl; and the corresponding dihydroxy acids in which the lactone ring is hydrolytically opened, and the pharmaceutically acceptable salts of said dihydroxy acids, and the lower alkyl and the phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the 4-R enantiomer of the trans racemate whose structure is shown above. 6. The compounds of claim 5 in which E is -CH2-CH2- or -CH=CH-, R1 is halogen in the 2-position, R2 is halogen in the 4-position, R3 is halophenyl-C1-3-alkoxy, halophenoxy or halophenyl in the 6-position. 7. The compounds of claim 6 in which A is hydrogen and R1 and R2 are chloro. 8. The compounds of claim 7 in which E is -CH=CH- and R3 is 4-fluorophenyl. 9. A hypocholesterolemic, hypolipemic, pharmaceutical composition comprising a pharmaceutical carrier and an effective hypocholesterolemic, hypolipemic amount of a compound of structural formula: EMI91.1 wherein A is H or methyl; E is a direct bond, -CH2-, -CH2-CH2-, -CH2-CH2-CH2-, or -CH=CH-; ; R1, R2 and R3 are each selected from H, halogen, C1-4 alkyl, C14 haloalkyl, phenyl, phenyl substituted by halogen, C1-4 alkoxy, C2-8 alkanoyloxy, C14 alkyl, or OR4 in which R4 is H, C2-8 alkanoyl, benzoyl, phenyl, halophenyl, phenyl C1-3 alkyl, C1-9 alkyl, cinnamyl, C1¯4 haloalkyl, allyl, cycloalkyl-C1-3 alkyl, adamantyl C13 alkyl, or substituted phenyl C1-3 alkyl in each of which the substituents are selected from phenyl C13 alkyl, C1-9 alkyl, cinnamyl, C1-4 haloakyl, allyl, cycloalkyl-C1-3 alkyl, adamantyl C1-3 alkyl, or substituted phenyl C1-3 alkyl in each of which the substituents are selec ted from halogen, C14 alkoxy, C14 alkyl, or C1-4 haloalkyl; or the corresponding dihydroxy acids in which the lactone ring is hydrolytically opened, or the pharmaceutically acceptable salts of said dihydroxy acids, or the lower alkyl and the phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all of said compounds being the 4-R enantiomer of the trans racemate whose structure is shown above. 10. The pharmaceutical compositions of claim 9 in which E is -CH2-CH2- or -CH=CH-, R1 is halogen in the 2-position, R2 is halogen in the 4-position, R3 is halophenyl-Cl 3-alkoxy, halophenoxy or halophenyl in the 6-position. .11. The pharmaceutical compositions of claim 10 in which A is hydrogen and R1 and R2 are chloro. 12. The pharmaceutical compositions of claim 11 in which E is -CH=CH- and R3 is 4-fluorophenyl. CLAIMS FOR AUSTRIA 1. A process for preparing compounds having the structure EMI94.1 where in E is a direct bond, -CH2- , -CH2-CH2-, -CH2-CH2-CH2- or -CH=CH-; R1, R2 and R3 are each selected from H, halogen, alkyl, C1-4 haloalkyl, phenyl, phenyl substituted by halogen, C1-4 alkoxy, C2-8 alkanoyloxy, C1-4 alkyl, or OR4 in which R4 is H, C2-8 alkanoyl, benzoyl, phenyl, halophenyl, phenyl C13 3 alkyl, C1-9 alkyl, cinnamyl, C1-4 haloalkyl, allyl, cycloalkyl-C1-3 alkyl, adamantyl C13 alkyl, or substituted phenyl C1-3 alkyl in each of which the substituents are selec ted from halogen, C14 alkoxy, C14 alkyl, or C14 haloalkyl; and the corresponding dihydroxy acids in which the lactone ring is hydrolytically opened, and the pharmaceutically acceptable salts of said dihydroxy acids, and the lower alkyl and the phenyl, dimethylamino or acetylamino-substituted lower alkyl esters of said dihydroxy acids; all said compounds being the 4-R enantiomer of the trans racemate whose structure is shown above, which comprises the aldol condensation of the dianion of acetoacetic ester with an aldehyde of the structure EMI95.1 followed by reduction of the 3-keto group to a hydroxyl, saponification of the ester, lactonization of the carboxyl with the 6-hydroxyl, if desired, reducing any double bonds in the bridging group E, and separating the cis and trans racemates and separation therefrom of the 4 (R) trans enantiomers. 2. The process of claim 1 for the preparation of the compound wherein: E is -CH2-CH2- or -CH=CH-; R1 is halogen in the 2-position; R2 is halogen in the 4-position; R3 is halophenyl-C1-3 alkoxy, balophenoxy or halophenyl in the 6-position. 3. The process of claim 2 for the preparation of the compound wherein: R1 and R2 are chloro. 4. The process of claim 3 for the preparation of the compound wherein: E is -CH=CH-, and R3 is 4-f luorophenyl.

Inventors: CRAGOE, EDWARD J., JR., HOFFMAN, WILLIAM F., NOVELLO, FREDERICK C., WILLARD, ALVIN K.
Applicants: MERCK & CO. INC.
Application year: 1980
Main classification: C07D309
IPC codes: A61K31, C07D309, A61P43, A61P31, C12N9, A61P3