OPTICALLY ACTIVE CEPHALOSPORIN ANALOGS, PROCESS FOR THEIR PREPARATION AND THEIR USE IN THE PREPARATION OF PHARMACEUTICAL COMPOSITIONS

Abstract: Optically active cephalosporin analogs represented by the general formula (I) wherein R¹ represents a hydrogen or a lower alkyl group, R² represents a hydrogen or a halogen atom, R₃ represents a hydrogen or protective group of carboxylic acid, and the hydrogens at the 6- and 7-positions have cis configuration, and the pharmaceutically acceptable salts thereof, are produced by optically selective deacylation of an optically inactive acylated analog. The compounds are useful as intermediates in the preparation of optically active acylated antimicrobial agents.

Description: Background of the Invention The present invention relates to optically active cephalosporin analogs and, more particularly, it pertains to optically active compounds of cephalosporin analogs represented by the general formula (I) EMI1.1 wherein R1 represents a hydrogen or a lower alkyl group, R2 represents a hydrogen or a halogen atom, R3 represents a hydrogen or protective group of carboxylic acid, and the hydrogens at the 6- and 7-positions have cis configuration, the pharmaceutically acceptable salts thereof and processes for producing the same. Heretofore, a carbacephem compound, which is named according to the nomenclature in J. Am. Chem. Soc. 96, 7584 (1974), wherein the sulfur atom of cephalosporin is substituted with a carbon atom and which has a 'substituted methyl group at the 3-position is described in the above reference and J. Med Chem. 20, 551 (1977). However, no compound of this type having strong antibacterial activity especially has been reported. The present inventors have succeeded in preparing. carbacephem compounds having various substituents at the 4-, 5- and 3-positions [The numbering system is as shown in general formula (l)j. The compounds are described in the specifications of Japanese Patent Applications No. 34696/78 (Japanese Published Unexamined Patent Application No.128591/79) (German Offenelegungsschrift 2911786, referred as "G.O:" hereinafter), 122403/78, 133072/78, 162005/78 and 8408/79. Further, the present inventors have succeeded in preparing novel acylated carbacephems which are new antibiotics having strong antibacterial activities. These are described in Japanese Patent Applications No. 34696/78 (Japanese Published Unexamined Patent Application No. 128591/79), 122402/78, 127027/78, 133071/78, 162006/78, 162007/78, 162008/78, 8409/79 and G.O. 2911787. However, cephalosporin analog mentioned above are prepared by synthetic methods using optically inactive starting compounds, and they are optically inactive dl lrepresented by ( compounds unless they have optically active acyl group. More specifically, compounds represented by the general formula (I) wherein the hydrogen atoms at the 6- and 7-positions have cis configuration are present as a mixture of equal amounts of the mirror image compounds represented by the formulae (I-1) and (I-2) EMI2.1 wherein R1, R2 and R3 have the same significance as defined above. However, no method of isolating one of these enantiomers has been reported. To this end, it has now ben found that one of the optically active mirror image compounds can be prepared and isolated. Summary of The Invention In accordance with the present invention, optically active compounds are prepared of cephalosporin analogs represented by the formula: EMI3.1 (wherein R1 represents a hydrogen or a lower alkyl group, R2 represents a hydrogen or a halogen atom, R3 represents a hydrogen or a protective group of carboxylic acid and the hydrogens at the 6- and 7-positions have cis configuration) and salts thereof. In the foregoing general formula (I), the lower alkyl group R1 is a straight or branched alkyl group having 1 to 5 carbon atoms such as methyl group, ethyl group, n-propyl group, i-propyl group, n-butyl group, i-butyl group, sec-butyl group, t-butyl-group, and the like. The ester group COOR3 is a group readily convertible to COOH employed in the chemistry of peniciliins and cephalosporins. The group R3, may be a straight-rhain or branched alkyl group having 1 to 5 carbon atoms such as methyl group, ethyl group, n-propyl group, i-propyl group, n-butyl group, i-butyl group, sec-butyl group, t-butyl group, and the like; a straight-chain or branched alkoxymethyl group having 1 to 5 carbon atoms such as methoxymethyl group, ethoxymethyl group, and the like; a straight or branched halogenated alkyl group having 1 to 5 carbon atoms such as chloromethyl group, 2,2,2-trichloroethyl group, 2,2,2-trifluoroethyl group, and the like; a lower alkylsulfonylethyl group such as methylsulfonylethyl group, ethylsulfonylethyl group, and the like; an arylmethyl group hiving 7 to 12 carbon atoms such as benzyl group, diphenylmethyl group, trityl group, triphenylmethyl group, and the like; a substituted arylmethyl group having 7 to 20 carbon atoms wherein the substituent is methoxy group, nitro group, or the like and the number of the substituents on the phenyl ring is 1 to 5; or a protective group of carboxylic acid represented by the general formula (11). EMI4.1 wherein R4 represents a straight-chain or branched lower alkyl group having 1 to 5 carbon atoms, a straight-chain or branched lower alkoxy group having 1 to 5 carbon atoms., or a phenyl group, and Rg represents a hydrogen or a straightchain or branched lower alkyl group having 1 to 5 carbon atoms. As the halogen atom, chlorine, bromine or iodine is exemplified, The optically active compounds of cephalosporin analogs represented by the general formula (I), that is, one of the enantiomers, arB prepared, according to the present invention, by an optically selective deacylation reaction using an enzyme end an optically inactive dl compound having an acyl group as a certain starting compound. The desired compound is obtained in a remarkably high yield by this method. A compound wherein an optically active acyl group is introduced to a dl form of a compound represented. by the general formula (I), referred to as Compound (I) hereinafter, that is, the compound represented by the general formula (A) EMI4.2 is separated to diastereoisomers (Japanese Patent Application No. 127027/78) -(G.O. 2911787) The optically active compounds obtained in the present invention are assumed to have the absolute structure represented by the general formula (In1), that is (6R, 7S), from various properties, strong antimicrobial activity of the acyl compounds as compared with the corresponding optically inactive dl-compound and the relationship between the absolute chemical structure of cephalosporins and activities thereof. These compounds are particularly useful as intermediates in the preparation of optically active acylated compounds which are strong antibacterial agents. In the following description, the optically active compounds are described with reference to the general formula (I-1). Additionally, the compounds in the following examples and reference examples are named according to the assumed absolute structural formula. Detailed Description of The Invention Optically active compounds of the cephalosporin analogs represented by the general formula (I) or compounds represented by the assumed absolute structural formula (In1) are produced by optically selective deacylation of a compound represented by the general formula (III) EMI5.1 wherein R represents a substituted or unsubstituted unsaturated six-membered carbocyclic or five-membered - heterocyclic group, wherein substituent represent hydroxy group, halogens/ nitro group or methansulfonamide group, X represents a hydrogen, an amino group, a hydroxy group or a lower alkyl group (referred to as Compound [III] hereinafter), R1, R2 and R3 have the same significance as defined above, and the hydrogens at the 6- and 7-positions have cis configuration. As the unsaturated six-membered carbocyclic and five-membered heterocyclic group, phenyl group, cyclohexenyl group, cyclohexadienyl group, thienyl group, furyl group pyrrolyl group, thiazolyl group, iso-thiazolyl group, oxazolyl group, iso-oxazolyl group, imidazolyl group, pyrazolyl group, triazolyl group, tetrazolyl group, pyridinyl group, and pyrazinyl group are exemplified. As the substituent, hydroxy group, halogens,nitro group, methansulfonamide group, and the like are mentioned. As the lower alkyl group, straight-chain or branched alkyl groups having 1 to 5 carbon atoms such as methyl group, ethyl group, n-propyl group, i-propyl group, n-butyl group, i-butyl group, sec-butyl group, t-butyl group and the like are mentioned. The optically selective deacylation of Compound 1III3 to obtain optically active Compound lI] is carried out in the presence of an enzyme obtained from a microorganism capable of producing optically active Compound (iJ by optically selective deacylation of Compound [iii). As the microorganism having an ability of optically selective deacylation, microorganisms belonging to the genus Aeromonas, Achromobacter, Arthrobacter, Acetobacter, Alcaligenes, Escherichia, Xanthomonas, Kluyvera, Gluconobacter, Clostridi-=m, Comamonas, Corynebacterium, Sarcina, Staphylococcus, Spirillum, Bacillus, Pseudomonas, Flavobacterium, Brevibacterium, Protaminobacter, Proteus, Beneckea, Micrococcus, Mycoplane and Rhodopseudomonas are used. The following strains are examples of the microorganism. Aeromononas hydrophila IFO 12634 Achromobacter aceris IFO 3320 Arthrobacter simplex ATCC 15799 Acetobacter aurantius IFO 3245, IAM 1812 Acetobacter ss. ATCC 21760 Alcaligenes faecalis ATCC 8750 Escherichia coli ATCC 11105 Escherichia coli ATCC 13281 Xanthomonas citri IFO 3835 Xanthomonas physalidicola IFO 13555 Kluyvera citrophila ATCC 21285 Gluconobacter liquefaciens ATCC 14835 Clostridium acetobutylicum IFO 3346 Comamonas sp. FERM-P 2410 Corynebacterium tritici IFO 1216 Sarcina lutea ATCC 9341 Staphylococcus aureus IFO 3060 Spirillum methamorphum IFO 12012 Bacillus megaterium ATCC 14945 Pseudomonas melanogenum ATCC 17808 Pseudomonas aeruginosa IFO 3451 Flavobacterium sp. ATCC 21429 Brevibacterium cerinum ATCC 15112 Protaminobacter alboflavus IFO 13221 Proteus rettgeri ATCC 9250 Beneckea hyperoptica ATCC 15803 Micrococcus luteus AHU 1427 Mycoplana bullata IFO 13267 Mycoplana dimorpha IFO 13213 Rhodopseudomonas spheroides ATCC 21286 For carrying out the optically selective deacylation reaction, the enzyme may be provided, more specifically, in any of the following forms: 1. As the culture liquor of the microorganism or treated matter thereof; 2. As cell bodies recovered from the culture broth by centrifugation which may be washed with saline water (usually about 1%), buffer solution and the like, or as a cell suspension; 3. As a disrupted cell suspension, i.e., a suspension of the cell bodies disrupted mechanically or chemically; 4. As a cell free extract, i.e., a liquid obtained by removing the disrupted cell bodies from the disrupted cell suspension; or 5. As a purified enzyme solution which is obtained by .recbvering the enzyme protein with ammonium sulfate from the cell free extract and subjecting the enzyme protein to gel filt:ation, ion-exchange cellulose column chromatography, ion exchange sephadex column chromato graphy, and the lik,.. Cells or the purified enzyme immobilized by a conventional method may be used. The reaction is carried out at a temperature of 0 to 400C, preferably 15-to 350C and at a pH of 5 to 8 in an inactive solvent which does not affect the reaction. As the solvent1 water is most preferably used. In order to dissolve the substitute or cephalosporin analogs, organic solvents such as acetone, methanol, ethanol, N,Ndimethylformamide, dinethylsulfoxide, and the like may be used. It is effective to add phosphate buffer, Veronal buffer or citric acid buffer to control the pH in the reaction Reaction time being influenced by the kind and concentration of enzymes, the kind and concentration of substrates, reaction temperature or reaction pH, is generally 30 minutes to 24 hours. It is most preferable to terminate the reaction when the reaction ratio reaches maximum. The concentration of cells is preferably 1 to 50 mg by dry weight per 1 ml of the reaction solution. When a purified enzyme is used, it is appropriate to use the amount of the enzyme having the same activity as that of the dry cell. The substrate Compound [III] is used in an amount of 0.5 to 50 mg per 1 ml of the reaction solution. In the event the microorganism utilized also produces an enzyme such as B-lactamdse, esterase or the like, which tend to prevent the desired reaction, such microorganisms can be mutated by known techniques to obtain a mutant strain which has a reduced productivity of the undesirable enzyme. Alternatively, inhibitors of such enzymes may be added in the reaction system to raise the reaction ratio. After the completion of the reaction, isolation of the desired compound is carried out by a conventional method employed in the isolation and purification of organic compounds from culture liquors such as absorption using various carriers, ion-exchange chromatography, gel filtration, liquid liquid extraction, and the like. Among the compounds represented by the general formula (I), the optically active compounds of the cephalospori: analogs represented by the general formula (I-3) EMI9.1 (wherein R1 and h have the same significance as defined above, Rl3 represents a protective group of carboxylic acid and the hydrogens at the 6- and 7-positions have cis configuration) may also be obtained by the esterification of the optically active cephalosporin analogs represented by the general formula (I-4) EMI9.2 (wherein R1 and R2 have the same significance as defined above, and the hydrogens at the 6- and 7-positions have cis configuration) by a conventional method, that is, the compound represented by t.he formula (I-3') EMI9.3 (wherein R2, R2 R2 and R'3 have the same significance as defined above) are obtained by the esterification of the compound represented by the formula (I-4') EMI9.4 (wherein R1 and R2 have the same significance as defined above) by a conventional method. Optically active compounds of the present invention, that is, Compound (i-li, themselves are expected to have antimicrobial activities and the acyl compounds of the optically active Compound lI] (Compound [I-1]) have much stronger antimicrobial activities than the acyl compounds of the corresponding optically inactive Compound 1I3. Examples of such compounds and antimicrobial activities thereof are described in Reference Examples. The present invention is explained by the following Examples. Example 1. Preparation of (+)-cis-7 amino-1-azabicyclo[4,2,0] oct-2-en-8-on-2-carboxylic acid 1(+)-cis-7 -amino-2-carboxy1-azabicyclo[4,2,0]oct-2-en-8-on]: (cis refers to the stereochemistry at 6-and 7 positions and the same shall apply hereinafter) EMI11.1 1-1. Preparation of disrupted cell suspension 1) Cultivation of a microorganism having an ability of optically selective deacylation As the seed strain, Xluyvera citrophila ATCC 21285 ±Biological properties are described in J. General Applied Microbiology 3, 28-31 (1957)] is used. As the seed medium, an aqueous solution containing 18 polypeptone, 1% yeast extract, 0.58 meat extract, 0.5t sodium glutamate and 0.25% sodium chloride and adjusted at a pH of 7.0 with 5N-NaOH is used. One loopful of the seed strain is inoculated into 10 mQ of the seed medium in, 50 mi of a large test tube and culturing is carried out at a temperature of 300C for 24 hours. The whole of the seed broth is inoculated into 300 ml of the culture medium in 2 l of an Erlenmeyer flask and culturing is carried out at a temperature of 300C with shaking. The composition of the main culture medium is the same as that of the seed medium. 2) Preparation of disrupted cell suspension After culturing for 24 hours, the culture broth is subjected to centrifugation to obtain cell bodies. The cells are washed twice with 50 mQ of0.9% saline solution and suspended in a concentration of 40 mg/mQ by dry weight in 1/30M phosphate buffer solution. Then, 10 mt of the cell suspension is put in 50 mQ of a large test tube and subjected to ultrasonic disintegration at 200 W for.2 minutes to obtain discupted cell suspension. In the treatment, ultrasonic disintegrator Model UR200P (product of Tomy Seiko Co., Ltd.) is used. 1-2. Preparation of a substrate solution In this step, 200 mg of (#)-cis-7-phenylacetamido- 1-azabicyclo[4,2,0]oct-2-en-8-on-carboxylic acid .¯¯¯ '- obtained as in GO 2911787 is added into 9 mQ of 1/30M phosphate buffer (pH 6.5). Since the compound is not dissolved, 2N-NaOH is added in a small portion and the mixture is again adjusted at a pH of 6.5 to dissolve the compound. Finally, deionized water is added to make 10 mQ of a solution. "GO" refers to German Offenlegungsschrift No., hereinafter. 1-3. Enzyme reaction In this step, 10 mQ of the disrupted cell suspension mentioned above is added in 10 mQ of the substrate solution and enzyme reaction is carried out at a temperature of 300C for 80 minutes. Time course of the reaction is illustrated in Table 1. Table 1 The amount of Reaction period Compound [1-13 Yield (minutes) produced (mg/mA) (Mol ratio, %) 10 2.0 33 20 2.6 43 40 2.9 48 60 3.0 50 80 3.0 50 As apparent from the Table 1, the reaction and yield are stationary after the conversion ratio of the mixture of the optically active isomers reaches 50% (mol ratio) 1-4. Isolation and Purification of the desired compound After the completion of the reaction, cells are removed by centrifugation from the reaction solution. The supernatant is adjusted at a pH of 3.0 with 2N-hydrochloric acid and charged on a column (2.6' cm width, 51 cm height) packed with 270 ml of Diaion HP-10 (product of Mitsubishi Kasei Co., Ltd.). Elution is carried out with deionized water and the eluate is collected in 5 mQ of fractions. The desired compound is eluted from 280 mQ to 315 mQ of the fractions. The fractions are concentrated under reduced pressure, lyophilized and dissolved in a small amount of a mixture of water and methanol (50:50 by volume, the same shall apply hereinafter). The solution is charged on a column (1.6 cm width, 64.5 cm height) packed with 130 ml of Sephadex LH-20 (Farmacia Fine Chemicals Co., Ltd.). Elution is carried out with a mixture of water and methanol (50:50). The eluate is collected in 5 mll of fractions. Fractions from 65 ml to 85 mQ are combined and concentrated under reduced pressure to remove methanol. Then, the residue is lyophilized to obtain 48 mg of a white powder. Properties of the product are as follows. -1 cm1 max : 1800, 1790, 1775, 1640, 1620 NMR(100M D2O-DSS)6: 6.46(1H,dd,J=3.5, 4.7Hz), 4,88(1H,d, J=5.2Hz), 4.06(1H,m), 2.5-1.5-(4H,M) It is revealed that the compound has one mole of hydrochloric acid and water. The properties of the compound coincide well with those of the corresponding d±-compound. The value of optical rotation is [&alpha;]D15 = +48 [c=0.5, in 1M phosphate buffer solution (pH 7.0)] which coincides well with the value in Example 2 below, [&alpha;]D15 =48.5 [c=0.5, in 1M phosphate buffer solution (pH 7.0)]. The compound shows a ninhydrin positive single spot at an Rf value of 0.22 on silica gel thin layer chromatography [thin layer plate Merck Art 5721 (product of E. Z4erck & Co.], solvent for development, isopropanol : acetic acid : water ¯ 4 : 1 : 13. The Rf value coincides with that of the optically inactive dQ-compound. Example 2. Preparation of (+)-cis- 7 -amino-1-azabicyclo[4,2,0] oct-2-en-8-on-2-carboxylic acid (Alternative method) 2-1. Preparation of disrupted cell suspension The same procedure as in Example 1-1 is repeated 2-2. Preparation of a substrate solution In this step, 100 mg of (+)-cis-7 -[(R)-2-phenyl- 2-aminoacetamido]-1-azabicyclo[4,2,0]oct-2-en-8-on-2carboxylic acid obtained as in GO 2911787 is dissolved in 5 m9 of 1/30M phosphate buffer solution (pH 6.5). 2-3. Enzyme reaction In this step, 5 mi of the disrupted cell suspension mentioned above are added in 5 mQ of the substrate solution and enzyme reaction is carried out at 300C for 24 hours. 2-4. Isolation and Purification 46 mg of a white powder is obtained by a similar method as in Exemple 1-4. Properties of the compound coincide well with those of the compound obtained in Example 1. [&alpha;]D15 = 48.5 [c=0.5, in 1M phosphate buffer solution .(pH 7.0)) Example 3. Preparation of (-)-cis-7B-amino-4a-methyl-l- azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid 3-1 Preparation of disrupted cell suspension A similar procedure as in Example 1-1 is repeated. 3-2. Preparation of a substrate solution A similar procedure as in Example 1-2 is repeated except that (#)-cis-7ss-phenylacetamido-4&alpha;-methyl-1-azabi cyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid obtained as in GO 2911787 is used. 3-3. Enzyme reaction A similar procedure as in Example 1-3 is repeated except that the disrupted cell suspension and the substrate solution obtained in 3-1 and 3-2 are used. Reaction ratio becomes stationary in one hour. The reaction is continued for 120 minutes. The yield is 50% (mol ratio) of the (t) substrate. 3-4. Isolation and Purification of the desired compound An almost similar procedure as in Example 1-4 is repeated. After the completion of reaction1 cells are removed by centrifugation from the reaction solution. The supernatant is charged on a column (2.5 cm width, 46 cm height) packed with 220 ml of Diaion HP-10. Elution is carried out with deionized water and the eluate is collected in 5 mQ of fractions. The desired compound is eluted in 200 mQ to 270 mi of fractions. The fractions are concentrated under reduced pressure, lyophilized, and dissolved in a small amount of water and methanol (50:50). - The solution is charged on a column (1.6 cm width, 64.5 cm height) packed with 130 mQ of Sephadex LH-20 and elution is carried out with a mixture of water and methanol (50:50). The eluate is collected in 5 ml of fractions. The fractions from 65 mt to 80 mQ are combined and concentrated to removemethanol. Then, the residue is lyophilized to obtain¯30.5 mg of a white powder. Properties of the compound are as follows. IR(Kbr)#maxcm-1: 1800, 1770(sh), 1760(sh), 1740, 1680, 1630 NMR(100M D2O-DSS)#: 6.16(1H,d,J=7.3Hz) J=4.9Hz), 3.86(lH,m), 2.64(lH,m), 1.9-1.4(2H,m), 1.10(3H,d,J=7.3Hz) It is revealed that the compound.is a potassium salt having 2 moles of water. The properties above coincide well with those of the corresponding di-compound. The compound shows a ninhydrin positive single spot at Rf=0.33 on a silica gel thin layer chromatography (the same silica gel as in Example 1-4 used). The Rf value coincides with that of the optically inactive di-compound. Optical rotation [&alpha;]D15 =-30 [c=0.5, in 1M phosphate buffer solution) The value coincides well with that in Example 4, [&alpha;]D15 =-30.8 [c=0.5, in 1M phosphate buffer solution (pH 7.0)). Example 4. Preparation of (-)-cis-75-amino-4a-methyl-1- azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid (Alternative method) 4-1. Preparation of disrupted cell suspension A similar procedure as in Example 3-1 is repeated. 4-2. Preparation of a substrate solution 100 mg of (+)-cis-7 -[(R)-2-phenyl-2-aminoacetamido3 4&alpha;-methyl-1-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid obtained as in GO 2911787 is dissolved in 5 mQ of 1/30M phosphate buffer solution (pH 6.5). 4-3. Enzyme reaction As in Example 1-3, 5 mZ of the disrupted cell suspension described above is added in 5 m± of the substrate solution and enzyme reaction is carried out at a temperature of 300C for 24 hours. 4-4. Isolation and Purification of the desired product A similar procedure as in Example 3-4 is repeated to obtain 55 mg of a white powder. Properties of the compound coincide well with those in Example 3. Optical rotation [&alpha;]D15 = -10.8 [c=o.5, in 1M phosphate buffer solution (pH 7.0)]. Example 5. Preparation of (+) -cis-7 -amino-l-azabicyclo [4,2,0] oct-2-en-8-on-2-carboxylic acid (Alternative method): 5-1. Cultivation of microorganisms As the seed microorganism, the following strains are used. Aeromononas hydrophila iFO 12634 Achromobacter aceris IFO 3320 Arthrobacter simplex ATCC 15799 Acetobacter aurantius IFO 3245 Acetobacter sp. ATCC 21760 Alcaligenes faecalis ATCC 8750 Escherichia coli ATCC 11105 Escherichia coli ATCC 13281 Xanthomonas citri IFO 3835 Xanthomonas physalidicola IFO 13555 Gluconobacter liquefaciens ATCC 14835 Gluconobacter dioxyacetonicus IFO 3271 Comamonas terrigena IFO 12685 Corynebacterium tritici iFO 1216 Sarcina lutea ATCC 9341 Staphylococcus aureus IFO 3060 Spirillum methanorphum IFO 12012 Bacillus megaterium ATCC 14945 Pseudomonas melanogenum ATCC 17808 Pseudomonas aeruginosa IFO 3451 Flavobacterium g. ATCC 21429 Brevibacterium cerinum ATCC 15112 Protaminobacter alboflavus IFO 13221 Proteus rettgeri ATCC 9250 Beneckea hyperoptica ATCC 15803 Mycoplana bullata IFO 13267 Mycoplana dimorpha IFO 13213 Rhodopseudomonas spheroides ATCC 21286 As a medium, an aqueous solution containing 1% meat extract, 1% peptone, 0.3E sodium chloride and 0.5t yeast extract and adjusted at a pH of 7.2 with SS-NaOH is used. One loopful seed strain is inoculated into 30 mA of the seed medium in 300 ml of an Erlenmeyer flask and culturing is carried out at a temperature of 300C for 24 hours. Cell bodies obtained by centrifugation from the culture broth are washed with 5 m± of 0.9% saline solution and again recovered by centrifugation therefrom. The cell is suspended in 1/30M phosphate buffer (pH 7.0) in a concentration of 20 mg/mi for dry weight. 5-2. Preparation of a substrate solution 150 mg of (#)-cis-7-[(R)-2-phenyl-2-aminoacetamido]- l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid prepared as in GO 2911787 is dissolved in 15 mQ of l/30M phosphate buffer (pH 7.0). 5-3. Enzyme reaction 0.5 m of each cell suspension prepared as in Example 5-1 and 0.5 mQ of the substrate solution are mixed and the mixture is allowed to react at a temperature of 300 for 20 hours. 5-4. Identification of the product It is possible to assay diastereoisomers of (#)-cis- 7-l(R)-2-phenyl-2-amlnoacetamldo]-l-azabicyclol4t2woloct-2- en-8-on-2-carboxylic acid by high speed liquid chromatography as described in GO 2911787. In this Example, diastereoisomer is quantitativeiy determined by such method. Quantitative determination of 7 -amino-l-azabicyclo[4,2,0]oct-2-en-8-on- 2-carboxylic acid produced in the above 5-3) is possible in the same manner. As apparent from Table 2, in the reaction solution a more polar diastereoisomer, i.e., (-)-cis-7-[(R)-2-phenyl 2-aminOacetamido3-1-azabicyclof4,2,0]oct-2-en-8-on-2- carboxylic acid is remained unchanged and a less polar isomer, i.e., (+)-cis-7-[(R)-2-phenyl-2-aminoacetamido]- 1-azabicyclo [4,2,0] oct-2-en-8-on-2-carboxylic acid is decreased. Corresponding to the decrease, a peak of (+)-cis7 -amino-l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid is formed. The unbalance of the decrease of (+)-cis-7-[(R)- 2-phenyl-2-aminoacetamido]-1-azabicyclo14,2,0]oct-2-en-8-on- 2-carboxylic acid and the formation of (+)-cis-7 -amino-lazabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid depends on the presence of ss-lactamase in the reaction system. Enzymes of all microorganisms in Table 2 have an ability of producing optically active compound having the absolute configuration (6R,7S) by selectively deacylating cephalosporin analogs. Table2 EMI20.1 <SEP> The <SEP> amount <SEP> of* <SEP> The <SEP> amount <SEP> of** <SEP> The <SEP> amount <SEP> of <tb> Microorganism <SEP> H <SEP> H <tb> <SEP> H2N# <SEP> #CHCON# <SEP> #CHCON# <tb> <SEP> H2N# <SEP> H2N# <tb> <SEP> (+) <SEP> COOH <SEP> (+) <SEP> COOH <SEP> (-) <SEP> COOH <tb> <SEP> produced. <SEP> decreased. <SEP> decreased. <tb> <SEP> (mg) <SEP> (mg) <SEP> (mg) <tb> Aeromonas <SEP> hydrophila <SEP> IFO <SEP> 12634 <SEP> 0.2 <SEP> 2.3 <SEP> 0 <tb> Achromobacter <SEP> aceris <SEP> IFO <SEP> 3320 <SEP> 0.4 <SEP> 2.0 <SEP> 0 <tb> Arthrobacter <SEP> simplex <SEP> ATCC <SEP> 15799 <SEP> 0.2 <SEP> 0.8 <SEP> 0 <tb> Acetobacter <SEP> aurantius <SEP> IFO <SEP> 3245 <SEP> 0.3 <SEP> 1.1 <SEP> 0 <tb> Acetobacter <SEP> sp. <SEP> ATCC <SEP> 21760 <SEP> 0.3 <SEP> 2.5 <SEP> 0 <tb> Alcaligenes <SEP> faecalis <SEP> ATCC <SEP> 8750 <SEP> 0.4 <SEP> 1.1 <SEP> 0 <tb> Escherichia <SEP> coli <SEP> ATCC <SEP> 11105 <SEP> 0.5 <SEP> 1.5 <SEP> 0 <tb> Escherichia <SEP> coli <SEP> ATCC <SEP> 13281 <SEP> 0.3 <SEP> 1.0 <SEP> 0 <tb> Xanthomonas <SEP> citri <SEP> IFO <SEP> 3835 <SEP> 0.9 <SEP> 2.5 <SEP> 0 <tb> Xanthomonas <SEP> physalidicola <SEP> IFO <SEP> 13555 <SEP> 1.3 <SEP> 2.5 <SEP> 0 <tb> Beneckea <SEP> hyperoptica <SEP> ATCC <SEP> 15803 <SEP> 1.1 <SEP> 2.4 <SEP> 0 <tb> Gluconobacter <SEP> liquefaciens <SEP> ATCC <SEP> 14835 <SEP> 0.8 <SEP> 2.4 <SEP> 0 <tb> Gluconobacter <SEP> dioxyacetonicus <SEP> IFO <SEP> 3217 <SEP> 1.2 <SEP> 2.4 <SEP> 0 <tb> EMI21.1 Comamonas <SEP> terrigena <SEP> IFO <SEP> 12685 <SEP> 0.1 <SEP> 0.3 <SEP> 0 <tb> Corynebacterium <SEP> tritici <SEP> IFO <SEP> 1216 <SEP> 0.2 <SEP> 0.4 <SEP> 0 <tb> Sarcina <SEP> lutea <SEP> ATCC <SEP> 9341 <SEP> 0.1 <SEP> 0.3 <SEP> 0 <tb> Staphylococcus <SEP> aureus <SEP> IFO <SEP> 3060 <SEP> 0.1 <SEP> 0.3 <SEP> 0 <tb> Spirillum <SEP> methamorphum <SEP> IFO <SEP> 12012 <SEP> 0.2 <SEP> 1.0 <SEP> 0 <tb> Bacillus <SEP> megaterium <SEP> ATCC <SEP> 14945 <SEP> 0.1 <SEP> 0.8 <SEP> 0 <tb> Pseudomonas <SEP> melanogenum <SEP> ATCC <SEP> 17808 <SEP> 1.3 <SEP> 2.5 <SEP> 0 <tb> Pseudomonas <SEP> aeruginosa <SEP> IFO <SEP> 3451 <SEP> 0.5 <SEP> 1.1 <SEP> 0 <tb> Flavobacterium <SEP> sp. <SEP> ATCC <SEP> 21429 <SEP> 0.5 <SEP> 1.5 <SEP> 0 <tb> Brevibacterium <SEP> cerinum <SEP> ATCC <SEP> 15112 <SEP> 0.4 <SEP> 0.8 <SEP> 0 <tb> Protaminobacter <SEP> alboflavus <SEP> IFO <SEP> 13221 <SEP> 1.2 <SEP> 2.5 <SEP> 0 <tb> Proteus <SEP> rettgeri <SEP> ATCC <SEP> 9250 <SEP> 0.1 <SEP> 0.2 <SEP> 0 <tb> Mycoplana <SEP> bullata <SEP> IFO <SEP> 13267 <SEP> 1.0 <SEP> 2.5 <SEP> 0 <tb> Mycoplana <SEP> dimorpha <SEP> IFo <SEP> 13213 <SEP> 1.3 <SEP> 2.5 <SEP> 0 <tb> Rhodopseudomonas <SEP> spheroides <SEP> ATCC <SEP> 21286 <SEP> 0.3 <SEP> 0.6 <SEP> 0 <tb> * When the yield is 100%, 1.4 mg of the compound is produced. ** The initial concentration is 2.5 mg. Example 6. Preparation of (+)-cis-7 -amino-l-azabicyclo[4,2,0] oct-2-en-s-on-2-carboxylic acid (Alternative method): Clostridium acetobutylicum IFO 3346 is inoculated in 100 int of Potatq Dextrose Broth (product of DIFCO Lab.). Air in the fermentor is replaced with sterilized nitrogen gas and the fermentor is sealed. Culturing is carried out at a temperature of 300C for 48 hours. After the cultivation, cells are recovered, washed with physiological saline solution and suspended in 2 mQ of 1/30M potassium phosphate buffer (pH 7.0). 0.5 ml of the substrate solution prepared as in Example 5-2) and the cell suspension are combined and allowed to react at a temperature of 300C for 20 hours. The reaction is monitored as in Example 5-4). (-)-cis-7- r (R)-2-phenyl-2-amlnoáceamidoJ-l-azabicyclot42o] oct-2-en-8-on-2-carboxylic acid is remained unchanged and only (+)-cis-7-[(R)-2-phenyl-2-aminoacetamido]-l-azabicyclo [4,2,0]oct-2-en-8-on-2-carboxylic acid is decreased The amount of the decrease is 1.0 mg, producing 0.2 mg of (+)-7 -amino-l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid. Example 7. Preparation of (-) -cis-7-amino-3-chloro-1-azabi- cyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid: 7-1. Preparation of disrupted cell suspension 1) Cultivation of a microorganism having an ability of optically selective deacylation As the seed strain, Kluyvera citrophila ATCC 21285 [Biological properties are described in J. General Applied Microbiology 3, 28-31 (1957)] is used. As the seed medium, an aqueous solution containing 1% polypeptone, 18 yeast extract, 0.5% meat extract, 0.5% sodium glutamate.and 0.25% sodium chloride and adjusted at a pH of 7.0 with SN-NaOH is used. One loopful seed strain is inoculated into 10 mL of the seed medium in 50 mt of a large test tube and culturing is carried out at a temperature of 300C for 24 hours. The whole of the seed broth is inoculated into 300 mQ of the culture medium in 2 t of an Erlenmeyer flask and culturing is carried out at a temperature of 30"C with shaking. The composition of the main culture medium is the same as that of the seed medium. 2) Preparation of disrupted cell suspension After culturing for 24 hours, the culture broth is subjected to centrifugation to obtain cell bodies.. The cells are washed twice with 50 mQ of 0.9% saline solution and suspended in. a concentration of 40 mg/mQ by dry weight in 1/30M phosphate buffer solution (pH 8.0). 7-2. Preparation of a substrate solution In this step, 200 mg of (+)-cis-7-phenylacetamido 3-chloro-l-azabicyclol4,2,0]oct-2-en-S-on-2-carboxylic acid obtained as in Reference Example 7 below is added into 9 mQ of 1/30M phosphate buffer (pH 8.0). Since the compound is not dissolved, 2N-NaOH is added in a small portion and the mixture is again adjusted to a pH of 8.0 to dissolve the compound. Finally, deionized water is added to make small of a solution. 7-3. Enzyme reaction In this step, 10 mQ of the disrupted cell suspension mentioned above is added in 10 mQ of the substrate solution and enzyme reaction is carried out at a temperature of 40"C for 80 minutes. Time course of the reaction is illustrated in Table 3. Table 3 The amount of Reaction period Compound [I-1] Yield (minutes) produced (mg/ml) (Mol ratio, %) 10 1.3 20 20 1.8 28 40 2.0 31 60 2.3 36 80 2.4 37 7-4. Isolation and Purification of the desired compound After the completion of the reaction, cells are removed by centrifugation from the reaction solution The supernatant is concentrated under reduced pressure to make 5 ml of solution. The solution is charged on a column (1.75 cm width, 42 cm height) packed with Diaion HP-10. Elution is carried out with deionized water. The desired compound is eluted from 90 mQ to 120 ml of the fractions. The fractions are concentrated under reduced pressure, to make 2 mQ of solution and the solution is adjusted to pH 3.5 with lN-hydrochloric acid to deposit crystals. The crystals are recovered by filtration, washed with a small amount of methanol and dried to obtain 38 mg of a white powder. Properties of the product are as follows. IR(KBr)#maxcm : 3200, 1800, 1790(sh), 1640(sh), 30, 1555 NMR(100M D2O-DSS)#: 4.47(1H,d.J=5.1Hz), 3.88(1H,m), 2.64(2H,m), 1.93(2H,m) Optical rotation [&alpha;]D25 =-2.7 (c=0.24, 1M phosphate buffer pH 7.0) Reference Example 1. Preparation of (+)-cis-7 -[2-(2-amino-4-thiazolyl)2-syn-methoxyiminoacetamido]-l-azabicyclo[4,2,0]oct-2-en-8on-2-carboxylic acid EMI25.1 In this Example, 76 mg (0.17 m mole) of trtityamino-4-thiazolyl)-2-syn-methoxyiminoacetic acid is dissolved in 1.52 mi of anhydrous dichloromethane and 17.3 mg (0.17 m mole) of triethylamine is added at a temperature of -150C. Then after adding 35.7 mg (0.17 m mole) of phosphorus pentachloride, the mixture is allowed to react with stirring at a temperature of -150C for 30 minutes. The reaction mixture is concentrated under reduced pressure and the residue is dissolved in 2 mQ of anhydrous tetrahydrofuran to make an acid chloride solution. Separa-ely, 28 mg (0.10 m mole) of the dihydrate of the potassium salt of -)-cis-7ss-amino-4&alpha;-methyl-l-azabi- cyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid obtained as in Example 3 is dissolved in 1.5 mQ cE a mixture of tetrahydrofuran and water (1:1) and 36.3 mg (0.36 za mole) of triethylamine is added to make a homogeneous solution. To the solution is added the acid chloride solution dropwise with stirring under ice cooling and the ,nixture is allowed do react for 45 minutes. Then, the reaction mixture is extracted four times with 3 ml of ethyl acetate. The ethyl acetate layer is washed with 5 ml of saturated saline solution, dried with saturated sodium sulfate, and concentrated under reduced pressure to obtain 107.1 mg of a crude acyl compound represented by the formula 1VII]. The product is dissolved in 4.5 ml of 50% acetic acid and the solution is stirred at a temperature of 50 to 550C for 45 minutes. After cooling to room temperature, the reaction solution is subjected to filtration to remove a deposited white precipitate and the cake is washed with 2 ml of 50% acetic acid. The washing and filtrate are combined and concentrated under reduced pressure. The residue is dissolved in a small amount of dimethylsulfoxide and charged on a column packed with 10 mQ of HP-10. Elution is carried out with water and methanol (5:1 to 2:1). Fractions showing an Rf value at 0.54 by silica gel thin layer chromatography (the same conditions as mentioned above are used) are combined and concentrated under reduced pressure to obtain 12.9 mg (yield 23.8%) of the desired product as a white powder. Properties of the product are as follows. M.P.: decompose at about 1800C [aj150= 27C (DXSO, c=0.5) IR(KBr)#maxcm-1: 1770, 1672, 1633, 1540 PMR(DMSO-d6)#: 9.26(1H,d.J=8.3Hz), 7.18(2H,s), 6.75 (1H,s), 6.31(1H,d,J=5.1Hz), 5.51(1H,d-d, J=8.3, 5.0Hz), 3.83(3E,s), 1.67(2H,m), 1.07(3H,d,J=7.3Hz) The values coincide well with those of the corresponding dQ- compound. From the strong antimicrobial activity, absolute configuration of this compound is assumed to be (4S,6R,7S). Reference Example 3. Antimicrobial activities of the compounds obtained in Reference Examples 1 and 2 are as follows. Heart Infusion Agar Dilution Method (pH 7.2) is used. The cephalosporin compound having the same acyl side chain corresponding to the dt-compound is used as a control. A: The compound obtained in Reference Example 1 A': The d±-compound corresponding to the compound obtained in Reference Example 1. 8: The compound obtained in Reference Example 2. B': The di-compound corresponding to the compound obtained in Reference Example 2. C: Cephalosporin compound represented by the following formula. EMI27.1 EMI28.1 Microorganism <SEP> MIC <SEP> ( g/ml) <tb> <SEP> A <SEP> A' <SEP> B <SEP> B' <SEP> C <tb> Staphylococcus <SEP> aureus <SEP> 209-p <SEP> 3.12 <SEP> 12.5 <SEP> 1.56 <SEP> 6.25 <SEP> 0.78 <tb> Staphylococcus <SEP> aureus <SEP> Smith <SEP> 6.25 <SEP> 25 <SEP> 6.25 <SEP> 12.5 <SEP> 1.56 <tb> Staphlococcus <SEP> epidermis <SEP> 12.5 <SEP> 25 <SEP> 3.12 <SEP> 12.5 <SEP> 1.56 <tb> Escherichia <SEP> coli <SEP> NIHJC-2 <SEP> 0.02 <SEP> 0.05 <SEP> 0.02 <SEP> 0.05 <SEP> 0.1 <tb> Escherichia <SEP> coli <SEP> GN2411-5 <SEP> 0.01 <SEP> 0.05 <SEP> #0.01 <SEP> 0.02 <SEP> 0.05 <tb> Escherichia <SEP> coli <SEP> Juhl <SEP> 0.02 <SEP> 0.1 <SEP> 0.02 <SEP> 0.05 <SEP> 0.05 <tb> Klebsiella <SEP> pneumoniae <SEP> 8045 <SEP> #0.006 <SEP> #0.006 <SEP> #0.01 <SEP> 0.01 <SEP> #0.01 <tb> Klebsiella <SEP> pneumoniae <SEP> Y-60 <SEP> 0.02 <SEP> 0.05 <SEP> 0.02 <SEP> 0.05 <SEP> 0.05 <tb> Serratia <SEP> marcescens <SEP> T-26 <SEP> 0.2 <SEP> 0.78 <SEP> 0.4 <SEP> 0.78 <SEP> 0.78 <tb> Serratia <SEP> marcescens <SEP> T-55 <SEP> 0.02 <SEP> 0.1 <SEP> 0.05 <SEP> 0.2 <SEP> 0.1 <tb> Proteus <SEP> mirabilis <SEP> 1287 <SEP> 0.01 <SEP> 0.02 <SEP> #0.01 <SEP> 0.01 <SEP> 0.02 <tb> Proteus <SEP> vulgaris <SEP> 6897 <SEP> #0.006 <SEP> 0.01 <SEP> #0.01 <SEP> 0.01 <SEP> #0.01 <tb> Proteus <SEP> morganii <SEP> KY <SEP> 4298 <SEP> 0.05 <SEP> 0.1 <SEP> 0.02 <SEP> 0.1 <SEP> 0.05 <tb> Proteus <SEP> rettgeri <SEP> KY <SEP> 4289 <SEP> #0.006 <SEP> #0.006 <SEP> #0.01 <SEP> 0.01 <SEP> #0.01 <tb> Pseudomonas <SEP> aeruginosa <SEP> #1 <SEP> 6.25 <SEP> 25 <SEP> 25 <SEP> 50 <SEP> 6.25 <tb> Pseudomonas <SEP> aeruginosa <SEP> 145 <SEP> 50 <SEP> 50 <SEP> 100 <SEP> #100 <SEP> 50 <tb> Pseudomonas <SEP> putida <SEP> 264 <SEP> 0.1 <SEP> 0.4 <SEP> 0.05 <SEP> 0.2 <SEP> 0.1 <tb> EMI29.1 In this Example, 131.3 mg (0.30 m mole) of 2-(2tritylamino-4-thiazolyl)-2-syn-methoxyiminoacetic acid is dissolved in 1 mQ of anhydrous dichloromethane and 4.1 of triethylamine is added at a temperature of -200C. Then, after adding 61.7 mg of phosphorus pentachloride, the mixture is allowed to react at a temperature of -200C for 30 minutes and concentrated under reduced pressure. The residue is dissolved in 1 ml of anhydrous tetrahydrofuran to make an acid chloride solution Separately, 40.2 mg (0.17 m mole) of the monohydrate of the hydrochloride of (+)¯cis-7-amino-5-azabScyclo [4,2,0]oct-2-en-8-on-2-carboxylic acid obtained as in Example 1 is dissolved in a mixture of 1 mQ of tetrahydrofuran and 1 mQ of water and 116.2 pQ of triethylamine is added. The acid chloride solution prepared above is added dropwise to the solution with stirring under ice coolinq and the mixture is allowed to react for one hour Then, the mixture is adjusted to a pH of 2.0 with 5% hydrochloric acid and extracted 3 times with 10 ml of ethyl acetate. The ethyl acetate layers are washed with 10 mQ of saturated saline solution, dried with saturated sodium sulfate and concentrated under reduced pressure to obtain 93 mq of a crude acyl compound. The product is dissolved in 10 mQ of 50# acetic acid and stirred at a temperature of 500C for 1.5 hours. The solution is cooled to room temperature and deposited whiteprecipitate is removed by filtration. The filtrate is concentrated and the residue is dissolved in a small amount of dimethylsulfoxide. The solution is cahrged on a column packed with 10 ml of HP-10 and elution is carried out with water to a mixture of water and methanol (1:2). Fractions showing an.Rf value of 0.3 by silica gel thin layer chromatography lplate: Merck Art. 5719 (product of E. Merck & Co.), solvent : butanol : acetic acid : water = 4 : 1 : Ii are combined and concentrated under reduced pressure to obtain 13.5 mg (yield 22.4%) as white crystals. Properties of the product are as follows. M.P.: 1720C (dec.) [&alpha;]D#=+32.6 (DMSO, c = 0.5) IR(KBr)#maxcm-1: 1765, 1660, 1630,1545 PMR(DMSO-d6)#: 9.26(1H,d), 7.19(2H,s), 6.75(1H,s), 6.28(1h,t), 5.50(lH,d-d, J=8.9, 4.7Hz), 3.83(3H,s), 2.5-1.0(4H,m) These values coincide well with those of the corresponding dQ-compound. From the strong antimicrobial activity, absolute configuration of this compound is assumed to be (6R,?S). Reference Examine 2. Preparation of (-)-cis-7a-[2-(2-amino-4-thiazolyl)- 3 2-syn-methoxyiminoacetamido]-4&alpha;-methyl-l-azabicyclo[4,2,0]oct- 2-en-8-on-2-carboxylic acid: Reference Example 4. Preparation of (s)-cis-7-azido-3-chloro-2-t- butylOxycarbonylwl-azabicyclof4,2,030ct-2-en-8-one: EMI31.1 The present compound is prepared according to the following processes a), b), c) and d). a) Preparation of (+)-cis-7-azido-3-phenylthio- l-azabicyclo[4,2,0]octane-8-on-2-carboxylic acid, t-butylester: EMI31.2 In this Example, 528 mg (2 m mole) of (+)-cis-7 azido-l-azabicyclot4,2,0]oct-2-en-8-on-2-oarboxylic acid, t-butylester prepared as in the me trod described in GO 2911786 is dissolved in 15 mQ of an.lydrous benzene ai.d 0.2 mQ (2 m mole) of thiophenol and 0.2 mQ (2 m mole) of piperidine are added. The mixture .s stirred at room temperature for 2 hours. After the completion of reaction, the reaction solution is washed with 10% citric acid and saturated saline solution and dried with anhydrous sodium sulfate. The solvent is removed by distillation under reduced pressure and the obtained oily residue is purified bv silica gel column chromatography using 30 g of silica gel and a solvent of ethyl acetate and n-hexane (1:4) to obtain 720 mg (96.3%) of the desired compound. Melting point: 77.5-78.0 C IR(KBr (cm-1). 2110, 1765, 1745 max NMR (CDCl3)#: 7.27-7.60(5H,m), 4.78(1H,d,J = 5Hz), 4.33(lH,s), 3.78-3.98(1H,m), 3.81(1H,s), 1.50-2.34(4H,m), 1.42(9H,s) b) Preparation of (#)-cis-7-azido-3-phenyl- sulfinyl-l-azabicyclo14,2,0)octane-8-on- acid, t-butylester: EMI32.1 In this Example, 480 mg (1.28 m mole) of 3-phenylthio compound obtained as in Reference Example 4-a) is dissolved in 50 ml of anhydrous chloroform and 240 mg (1.41 m mole) of m-chloroperbenzoic acid under ice cooling. The mixture is allowed to react with stirring under ice cooling for 30 minutes. The reaction mixture is washed with saturated sodium bicarbonate and saturated sodium chloride and dried with anhydrous sodium sulfate. The solvent is removed by distillation under reduced pressure to obtain 500 mg (99.9%) of the desired compound. Melting point: 95.5-96.5 C IR#maxKBr (cm-1): 2120, 2100, 1780, 1735, 1035 NMR (CDCl3)#: 7.55-7.91(5H,m), 4.87(1H,d,J = 4.6Hz), 4.05(1H,s), 3.90-4.10(1H,m), 3.10(1H,s), 1.70-2.84(4h,m), 1.30(9H,s) c) Preparation of (+)-cis-7-azido-3-chloro-3- phenylsulfinyl-l-azabicyclo[42oloctane-8 on-2-carboxylic acid, t-butylester: EMI33.1 In this Example, 109 mg of sulfoxide compound obtained as in Reference Example 4-b) is dissolved in 1 mt of anhydrous methylene chloride and 23.5 mg (0.42 m mole) of calcium oxide is added. After adding 27 for (0.34 m mole) of sulfinyl chloride, the mixture is allowed to react with stirring under ice cooling for one hour. The reaction mixture is washed with 10% citric acid, saturated sodium bicarbonate and saturated sodium chloride solution and dried with anhydrous sodium sulfate. The solvent is removed by distillation and the obtained-oily residue is subjected to silica gel chromatography using 5 g of silica gel and a solvent of ethyl acetate and n-hexane (1:5) to obtain 66.5 mg (56.1%) of the purified desired oily compound. IR#maxCDCl3 (cm 2120, 1770, 1735, 1055 max NMR (CDCl3)#: 7.53-8.00(5H,m), 4.90(1H,d,J = 5Hz), 4.43(1H,s), 4.15-4.35(lH,m), 1.83-2.85 (4H,m), 1.38(9H,s) d) Preparation of (+)-cis-7-azido-3-chloro-l- azabicyclof4,2,0loct-2-en-8-on-2-carboxylic acid, t-butylester: EMI34.1 In this Example, 1.3 g (3.06 m mole) of 3-chloro-3phenylsulfinyl compound obtained as in Reference Example 4-c) is heated under reflux with 100 mQ of carbon tetrachloride for 6 hours. After the completion of reaction, the reaction solution is subjected to distillation under reduced pressure to remove the solvent. The obtained residue is subjected to purification by silica gel chromatography using 100 g of silica gel and a solvent of ethyl acetate and n-hexane (1:5) to obtain 596 mg (65.2b). of the desired compound. Melting point: 96-970C IRmXBr (cm-1): 2120 , 1765, 1735, 1630 PMR(CDCl3)#: 4.93(1H,d,J = 5Hz), 3.72(1H,m), 2.56 2.70(2h,m), 1.86-2.32(2H,m), 1.55(9H,s), CMR (CDCl3)#: 127.7, 125.0 Reference Example 5. Preparation of (+)-cis-7-amino-3-chloro-l- azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid, t-buthylester: EMI34.2 In this Example, 350 mg (1.17 m mole) of the azido compound obtained as in Reference Example 4 is dissolved in 20 mQ of ethanol and 1.2 mQ of IN-hydrochloric acid and 70 mg of 108 palladium-carbon. Hydrogen gas is passed through the mixture at room temperature and atmospheric pressure for 3 hours and palladium-carbon is removed by filtration. The filtrate is concentrated under reduced pressure to obtain a solid. The solid is dissolved in water and washed with ether. After adding sodium bicarbonate to make weakly alkaline, the water layer is extracted with ethyl acetate. The ethyl acetate layer is washed with saturated saline solution and dried with anhydrous sodium sulfate. The solvent is removed by distillation under reduced pressure to obtain 218.4 mg (68.4%) of a powder of the desired compound. Melting point: 102.5-104.5 C IR#KBr (cm-1 max ): 1770, 1720, 1620 NMR (CDCt3)6: 4.43(1H,d,J = 5Hz), 3.52-3.90(lH.m). 2.52-2.72(2H,m), 2.22(2H,br), 1.87-2.17 (2H,m), 1.55(9H,s) Reference Example 6. Preparation of the trifluoroacetate of (i)-cis-7- amino-3-chloro-l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid: EMI35.1 In this Example, 102.2 mg (0.31 m mole) of the amino-ester compound obtained as in Reference Example 5 and 1 mb of trifluoroacetic acid is added under ice cooling. The mixture is stirred at room temperature for 30 minutes. The solvent is removed by distillation under reduced pressure to obtain an oily residue. To the residue is added ether and the resulted powder is recovered by filtration. The powder is lyophilized to obtain 75.5 mg (60.9 ) of the desired compound. Melting point: 208-220 C (dec.) IR#maxKBr (cm-1): 1795, 1630 Reference Example 7. Preparation of (+)-cis-7-phenylacetamido-3-chlorol-azabicyclo[4,2,0]cot-2-en-8-on-2-carboxylic acid: EMI36.1 In this Example, 150 mg (0.45 m mole) of the trifluoroacetate of (#)-cis-7-amino-3-chloro-l-azabicyclo- 14,2,0]oct-2-en-8-on-2-carboxylic acid prepared as in Reference Example 6 is dissolved in a mixture of 2 ml of water and 2 mQ of acetone and 134 mg (1.5 m mole) of sodium bicarbonate is added to the solution to make the solution homogeneous, To the mixture is added 0.5 mi of phenylacetyl- chloride under cooling in one hour and the mixture is stirred for 3 hours. The reaction mixture is adjusted to a pH of 2 with 1N hydrochloric acid and extracted 5 times with 2 mQ of ethyl acetate. The extract is concentrated under reduced pressure and dried to obtain 80 mg (55.0%) of the desired compound. IR#maxKBr (cm-1): 1790, 1705, 1630, 1560 NMR (CD3OD)#: 7.29(5H,s), 5.36(1H,d,J = 5Hz), 3.79-3.99 (lH,m), 2.56-2.75(2H,m), 1.17-2.02(2H,m) Reference Example 8. Preparation of (+)-cis-7-I(R)-2-phenyl-2-tbutyloxy- carbonylamino]-3-chloro-l-azabicyclo[4,2,0]oct-2-en-8-on-2carboxylic acid, t-butylester: EMI37.1 In this Example, 150 mg (0.55 m mole) of (i)-cis-7- amino-3-chloro-l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid, t-butylester obtained as in Reference Example 5 is dissolved in 3 mi of anhydrous methylene chloride. Separately, 166 mg (0.66 m mole) of N-t-butyloxycarbonyl-(R) phenylglycine is dissolved in 5 mQ of anhydrous tetrahydro furan and the solution is cooled at a temperature of -15 to -100C. Then, 0.66 mQ of lN-N-methylmorpholine in tetrahydrofuran and 0.66 mQ of lN-isobutyl chloroformate in tetrahydrofuran are added dropwise to the solution and the solution is stirred at a temperature of -15 to -10 C for 20 minutes. Under the same temperature, the amine solution prepared above is added dropwise to the solution and the temperature is raised gradually to room temperature. The mixture stirred at room temperature overnight. After adding 10 m± of methylene chloride, the reaction mixture is washed with 10% citric acid solution, saturated sodium bicarbonate and saturated saline solution, dried with anhydrous sodium sulfate and concentrated under reduced pressure. The concentrate is subjected to silica gel chromaography using 20 g of silca gel (Wako-gel C 200, product of' Wako Junyaku Co., Ltd.) and a solvent of ethyl acetate anR n-hexane (1:5 by volume, the same shall apply hereinafter) to obtain 124 mg (yield 44.68) of a mixture of the diastf.reoisomers of.the desired compound. Propertie of the product are as follows. IR#maxKBr (cm-1): 1780, 1730, 1680, 1655, 1550 NrGR (CDCQ3)6: 7.34(5/2H,s), 7.31(5/2H,s), 6.93(1H,m), 5.63(1H,m), 5.30(1/2H,dd,J = 5.4, 6.8Hz), 5.11-5.22(3/2H,m), 3.76-3.91(lH,m), 2.33-2.66(2H,m), 1.52(9H,s), 1.41(9H,s), 0.92-1.97 (2H,m) Reference Example 9. Preparation of (6R,7S) 7-(R)-phenylglycinamido 3-chloro-l-azabicyclof4,2,030ct-2-en-8-on-2-carboxylic acid (A) and (6S, 7R) 7- (R) -phenylglycinamido-3-chloro-1azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid: EMI38.1 in this Example, to 128.4 mg (0.25 m mole) of (#)-cis-7-(2-phenyl-2-t-buthylloxycarbonylaminoacetamido)-3- chloro-l-azabicyclo[4,2,0]oct-2-enm-8-on-2-carboxylic acid, t-butylester obtained as in Reference Example 8 is added 1 mQ of methylene chloride and 1 mQ of trifluoroacetic acid under ice cooling and the mixture is stirred under ice cooling for 1.5 hours. The solvent is removed by distillation under reduced pressure and the obtained oily productis subjected to high speed liquid chromatography using Micro-Bondapack C-18 (product of Waters, Co.) and a solvent of 7% methanol and 0.2N KH2P04 to separate diastereoisomers. Each of the eluted fractions are concentrated under reduced pressure and the concentrates are subjected to desaltation with Diaion HP-10 and a solvent of methanol and water (1:1) to obtain 9.4 mg of a more polar isomer (B) and 7.6 mg of a less polar isomer (A). Total yield 19.18 More polar isomer (B) [&alpha;]D21 : -75.8 (c = 0.4, H2O) Melting point: more than 3000C (brown colored) IR#KBr (cm-1 max ): 1765, 1700, 1550 NMR (D2O)#: 7.49(5H,s), 5.16(1H,d,J = 4.7Hz), 5.05(1H,s), 3.78-4.03(1H,m), 2.53-2.67(2H,m), 1.26-2.09 (2H,m) Less polar isomer (A) [&alpha;]D21 : +34.0 (c = 0.35, H2O) Melting point: more than 3000C (brown colored) IR#KBr (cm-1): 1770, 1700, 1630 max NMR (D2O)#: 7.51(5H,s), 5.36(1H,d,J = 4.6Hz), 5.19(1H,s), 3.83-4.00(1H,m), 2.41-2.56(2H,m), 1.49-1.76 (1H,m), 1.14-1.45(1H,m) The absolute structure of the isomer (A) is assigned as (6R,7S) based on strong antibacterial activity of the less polar isomer (A) as mentioned below. Reference Example 10. Preparation of (6R,7S) 7-(R)-phenylglycinamido3-chloro-l-azabicyclo[4,2,0]oct-2-en-8-on-2-carboxylic acid (alternative method): EMI39.1 1)-1) Preparation of cell suspension a Cultivation of a microorganism having an ability of :ptically selective acylation. A3 a seed strain, Pseudomonas melanogenum ATCC 17308 [Biological properties are described in Journai of the Agricultural Chemical Society of Japan 37, 71(1963)) is used. s s the seed medium, an aqueous solution containing 1% polypeptone, 1% yeast extract, 0.5 meat extract, 0.5% sodium glutamate and 0.25% sodium chloride and adjusted at a pH of 7.0 with 5N-NaOH is used. One loopful seed strain is.inoculated into 10 mQ the seed medium in 50 mt of a large test tube and culturing is carried out at a temperature of 300C for 24 hours. The whole amount of the seed medium is put into 300 mQ of the culture medium in 2 l of Erlenmeyer flask and culturing is carried out at a temperature of 300C. The composition of the culture medium is the same as that of the seed medium. b) Preparation of cell suspension After culturing for 24 hours, cell bodies are recovered from the culture broth by centrifugation and washed 2 times with 50 ml of 0.9% saline solution. The cells-are suspended in a concentration of 20 mg/mQ by dry weight in1/30M phosphate buffer (pH 6.5). 10-2) Preparation of a substrate solution 200mg of the trifluoroacetate of (#)-cis-7-amino-3-chloro-l-azabicyclo [4,2,0]oct-2-en-8-on-2-carboxylic acid (starting compound a) obtained as in reference example 6 and 800 mg of the hydrochloride of D-phenylglycinemethylester (starting compoud b ) are added in 9 mQ of 1/30N1 potassium phosphate buffer (pH 6.5). SN-KOH is added in a small portion and the mixture is again adjusted to a pF of 6.5 to dissolve two starting compounds. Finally, deionized water is added to make 10 mQ of a solution. 10-3) Enzyme reaction In this step, 10 ml of the cell suspension is added to 10 m of the substrate solution and enzyme reaction is carried out at a temperature of 300C for 1.5 hours. The reaction is monitored by high speed liquid chromatography using TRI ROTA (product of Nippon Bunko Co. Ltd.) and Prepack column Nucleosil 10C18 (product of Gaschro Xogyo Co., Ltd.). Elution is carried out with 7% methanol-0.2M KH2PO solution. Reaction reaches maximum in a yield of 90% to the starting compound a in 1.5 hours. 10-4) isolation and Purification of the desired compound: After the completion of reaction, cell bodies axe removed from the reaction solution by centrifugation. The supernatant is concentrated under reduced pressure and charged on a column (1.6 cm width, 50 cm height) packed with Diaion HP-10. After adding 200 mQ of deionized water, elution is carried out with 25% aqueous methanol solution. Then, the fractions containing the desired compound are concentrated under reduced pressure to make a 5 mQ of concentrate. The concentrate is charged on a column (1.6 cm width,. 64.5 cm height) packed with 130 mQ of Sephadex-LH2O and elution is carried out with a solvent of water and methanol (50:50). The desired compound is eluted in 55 mL to 75 mQ of fractions.. The fractions are concentrated under reduced pressure and lyophilized to obtain 78 mg of a white powder. Properties of the product coincide with the less polar isomer (A) in Reference Example 9. Antibacterial activities of the compounds obtained in Reference Examples 9 and 10 are shown in the following table. Heart-Infusion Agar Dilution Method (pH 7.2) is used. Cefazolin is used as a control. EMI42.1 <SEP> MIC <SEP> ( g/ml) <tb> <SEP> The <SEP> less <SEP> polar <SEP> isomer <SEP> (A) <SEP> The <SEP> more <SEP> polar <tb> Microorganism <SEP> obtained <SEP> in <SEP> Referance <SEP> isomer <SEP> (B) <tb> <SEP> Cefazolin <SEP> Example <SEP> 9 <SEP> and <SEP> the <SEP> com- <SEP> obtained <SEP> in <tb> <SEP> pound <SEP> obtained <SEP> in <SEP> Reference <tb> <SEP> Reference <SEP> Example <SEP> 10 <SEP> Example <SEP> 9 <tb> Staphylococcus <SEP> aureus <SEP> 209-p <SEP> #0.05 <SEP> 0.1 <SEP> 100 <tb> Staphylococcus <SEP> aureus <SEP> Smith <SEP> 0.4 <SEP> 1.56 <SEP> < 100 <tb> Staphylococcus <SEP> epidermis <SEP> 0.78 <SEP> 1.56 <SEP> < 100 <tb> Escherichia <SEP> coli <SEP> NIHJC-2 <SEP> 1.56 <SEP> 1.56 <SEP> < 100 <tb> Escherichia <SEP> coli <SEP> Juhl <SEP> 1.56 <SEP> 1.56 <SEP> < 100 <tb> Klebsiella <SEP> pneumoniae <SEP> 8045 <SEP> 0.78 <SEP> 0.2 <SEP> 100 <tb> Klebsiella <SEP> pneumoniae <SEP> Y-60 <SEP> 3.12 <SEP> 6.25 <SEP> < 100 <tb> Serratia <SEP> marcescens <SEP> T-26 <SEP> < 100 <SEP> < 100 <SEP> < 100 <tb> Serratia <SEP> marcescens <SEP> T-55 <SEP> 50 <SEP> 6.25 <SEP> < 100 <tb> Proteus <SEP> mirabilis <SEP> 1287 <SEP> 12.5 <SEP> 3.12 <SEP> < 100 <tb>

Claims: WHAT IS CLAIMED IS: 1. Optically active cephalosporin analogs represented by the general formula (I) EMI43.1 wherein R1 represents a hydrogen or a lower alkyl group, h represents a hydrogen or a halogen atom, R3 represents a hydrogen or protective group of carboxylic acid, and the hydrogens at the 6- and 7-positions have cis configuration, and the pharmaceutically acceptable salts thereof. 2. The compound according to claim 1 wherein R1 and R2 are hydrogens. 3. The compound according to claim 2 wherein R3 is a hydrogen, that is, (+)-cis-7-amino-l-azabicyclo [4,2,0] oct2-en-8-on-2-carboxylic acid. 4. The compound according to claim 1 wherein R1 is a methyl group and h is a hydrogen. 5. The compound according to claim 4 wherein R3 is a hydrogen. 6. The compound according to claim 5 wherein the methyl group has the same configuration with the hydrogens at the 6- and 7-positions, that is, (-)-cis-78-amino-4a- methyl-l-azabicyclo [,2,0] oct-2-en-8-on-2-carboxylic acid. 7. The compound according to claim 1 wherein R1 is a hydrogen and h is a chlorine. 8. The compound according to claim 7 wherein R3 is a hydrogen, that is, (-) -cis-7-amino-3-chloro-l-azabicyclo [4,2,0] oct-2-en-8-on-2-carboxylic acid. 9. A process for producing optically active compound represented by the general formula (I) EMI44.1 wherein R1 represents a hydrogen or a lower alkyl group, R2 represents a hydrogen or halogen atom, R3 represents a hydrogen or protective group of carboxylic acid, and the hydrogens at the 6- and ?-positions have cis configuration, which comprises reacting a compound represented by the formula (III) EMI44.2 wherein R represents a substituted or unsubstituted unsaturated six-membered carbocyclic or five-membered heterocyclic group, wherein substituent represent hydroxy group, halogens,nitro group or methansulfonamide group, X represents a hydrogen, an amino group, a hydroxy group or a lower alkyl group, R1, R2 and R3 have the same significance as defined above, and the hydrogens at the 6- and 7-positions have cis configuration with an enzyme capable of selective optical deacylation and thereafter recovering said optically active compound. 10. The process according to claim 9 wherein said enzyme is obtained from a microorganism belonging to the genus Aeromonas, Achromobacter, Arthrobacter, Acetobacter, Alcaligenes, Escherichia, Xanthomonas, Xluyvera, Gluconobacter, Clostridium, Comamonas, Corynebacterium, Sarcina, . Staphylococcus, Spirillum, Bacillus, Pseudomonas, Flavobacterium, Brevibacterium, Protaminobacter, Proteus, Beneckea , Micrococcus, Mycoplana or ChodoPseudomonas. 11. The process according to claim 10 wherein said enzyme is provided to said reaction in the form of a purified enzyme solution, cell bodies recovered from a culture broth, a cell suspension, a disrupted cell suspension, a cell free extract, or a culture liquor of the microorganism. 12. Pharmaceutical composition with antimicrobial activity comprising a compound according to any of claims 1 to 8 together with conventional diluents and/or carriers and/or adjuvants.

Inventors: HASHIMOTO, YUKIO, HIRATA, TADASHI, KIMURA, KAZUO, KOBAYASHI, SHIGERU, MATSUKUMA, IKUO, OGASA, TAKEHIRO, TAKASAWA, SEIGO, YOSHIIE, SHIGEO
Applicants: KYOWA HAKKO KOGYO CO., LTD
Application year: 1980
Main classification: C07D471
IPC codes: C12P17, C07D463