CHROMANOL DERIVATIVES, A PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS COMPRISING THEM

Abstract: Compounds of the formula (I): wherein the pyrrolidino and OR moieties are trans and wherein R is a hydrogen atom an alkyl group of 1 to 3 car­ bon atoms or an acyl group of 1 to 8 carbon atoms; or a pharmaceutically acceptable acid addition salt thereof; are anti-hypertensive agents. Their preparation and formula­ tion is described.

Description: Chromanol derivatives a process for their preparation and their use in pharmaceutical compositions British Patent Specification No. 1495526 discloses a large group of derivatives of trans- 3 -bydroxy-4- aminochroman which have blood pressure lowering activity. British Patent Specification No. 1511187 discloses a further group of derivatives of trans-3-hydroxy-4-aminochroman which also have blood pressure lowering activity. British Patent Specification No. 1511187 also disclosed that 6-cyano-3, 4-dihydro-2,2-dimethyl-trans-4-piperidino-2H-benzo[b] pyran-3-ol was a useful chemical intermediate although it was not suggested to be of particular use as an anti hypertensive agent in its own right. It has been found that trans-3-hydroxy-4-aminochromans such as the favoured 6-nitro compounds previously described as having vasodilatory activity also have unwanted cardiac effects. Clearly it is desirable to have compounds that has a better therapeutic ratio as judged by the separation of desired vasodilatory effects from undesired cardiac effects. It has now been found that 6-cyano-3,4-dihydro-2, 2-dimethyl-trans-4-pyrrolidino-2R-ben2o [b jpyran-3-ol (and especially the (+)-isomer thereof) and certain derivatives have such desirable properties. Accordingly the present invention provides the - compounds of the formula (I): EMI2.1 wherein the pyrrolidino and OR moieties are trans and wherein R is a hydrogen atom, an alkyl group of 1 to 3 carbon atoms or an acyl group of 1 to 8 carbon atoms1 or a pharmaceutically acceptable acid addition salt thereof. Suitable alkyl groups R in relation to formula (I) are the methyl, ethyl and n-propyl groups of which the methyl group is most suitable. Suitable acyl 'groups R in relation to formula (I) are unsubstituted carboxylic acyl groups much as unsubstituted aliphatic acyl or benzoyl. Preferably R in relation to formula (I) is a hydrogen atom. The compounds of the formula (I) may be provided in the form of a mixture of stereoisomers or as a single stereoisomer.It is particularly convenient to prepare and use the compounds of the formula (I) as racemic mixtures. Most of the desirable biological activity of the compounds of the formula (I) is found in the (+)-isomer of the compound wherein R is hydrogen and in the derivatives thereof wherein R is alkyl or acyl. Thus in a preferred aspect this invention provides such (+)-isomers (that is when effectively free of the (-)isomer). Suitable acid addition salts of the compounds o the formula (I) are those with pharmaceutically acceptable inorganic or organic acids such as hydrochloric, hydrobromic, phosphorIc, sulphuric, methanesulphonic, toluenesulphonic, acetic, propionic, succinic, citric, tartaric, mandelic, lactic, gluconic or the like acid. The present invention also provides a process for the preparation of a compound of the formula (I) or a pharmaceutically acceptable salt thereof which process comprises the reaction of the compound of the formula (it1: EMI3.1 with pyrrolidine and thereafter optionally reacting the thus produced compound of the formula (I) wherein R is a hydrogen atom and thereafter optionally alkylating or acylating said compound, resolving any of said compounds and/or salifying any of the resulting compounds of formula (I). The trans- isomer is specifically formed. The reaction of the epoxide may be carried out at any non-extreme low, medium or high temperature (for example, -lO0C to 2O00C) but in general ambient or slightly elevated temperatures are most suitable (for example 120 to 100 C). The reaction is normally carried out in a solvent such as a lower alcohol or lower ketone, for example methanol, ethanol, propanol, acetone or methylethylketonP It has been found that the reaction proceeds smoothly if carried out in refluxing ethanol. The desired product may be obtained from che reaction mixture by removal of the solvent which is normally accomplished by evaporation under reduced pressure. The initial product may contain some epoxide. This may be separated by dissolving the reaction product in ethyl acetate and extracting into dilute acid. If desired the solvent may be evaporated at this stage but it is usually more convenient to basify, back extract into ethyl acetate and recover by evaporation at reduced pressure. If a salt is desired this product (the free base) may be dissolved in diethyl ether containing a little ethanol and treated with a solution of the acid for example in diethyl ether. The desired salt may then be collected by filtration. The compound of the formula (II) may be prepared and reacted in situ, for example from a corresponding bromohydrin. Etherification of the initially produced compound of the formula (I) wherein R is a hydrogen atom may be effected in conventional manner such as reaction with an alkyl iodide in the presence of a base such as potassium tert-butoxide in an inert solvent such as toluene. Preparation of esters of the compounds of formula (I) wherein R3 is hydrogen may be by such conventional methods of esterification as reaction with an acylating agent optionally in the presence of an acid acceptor. Suitable acylating agents include acid halides such as a bromide or chloride by reaction with an acid in the presence of a condensation promoting agent such as dicyclohexylcarbodiimide or its chemical equivalent or by reaction with an acid anhydride. Such reactions are generally carried out in a non-hydroxylic solvent at a non-extreme temperature. Resolution of a compound of the formula (I) may be effected by forming a salt with an optically active acid such as optically active tartaric acid and fraction211y crystallising from a suitable solvent such as ethanol. In a further aspect the product invention also provides a pharmaceutical composition which comprises a compound of this invention and a pharmaceutically acceptable carrier. The compositions of this invention are most suitably adapted for oral administration although adaptation for other modes of administration such as by injection, for example by intra venous injection for heart -failure are also suitable. In order to obtain consistency of administration it is preferred that the compositions of this invention are in the form of a unit-dose. Suitable unit dose forms include tablets, capsules and powders in sachets or vials Such unit dose forms aptly contain from 1 to 100 my of the compound of this invention and more usually from 2 to 50 mg, for example 5 to 25 mg such as 6, 10, 15 or 20.-,g, Such compositions may be administered from 1 to 6 times a day, more usually from 2 to 4 times a day, in a manner such that the daily dose is from 5 to 200 mg for a 70 kg human adult and more aptly from 10 to 100 mg. Shaped oral dosage compositions are favoured composition aspects. The compositions of this invention may be formulated in conventional manner, for example in a manner similar to that used for known antihypertensive agents such as hydrallazine. In addition such compositions may contain further active agents such as other anti-hypertensive agents, diuretics and p-blocking agents. The following Exampies illustrate the invention. EXAMPLE 1 6-Cyano-3,4-dthydro-2,2-dlmethyl-trans-4-yrrolidino-2H-benzo- [b(-pyran-3-ol hydrochloride 4-Cyanophenol (19.60 g), sodium hydroxide (9.90 g), 0% benzyltrimethylainmonium hydroxide in methanol (34.50g) and 3-methyl-3-chlorobutyne (25.50 g) were stirred in water (150 ml) and dichloromethane (150 ml) for 5.5 days at room temperature. After separation of the layers, the aqueous layer was extracted twice with chloroform, and the combined organic phase evaporated leaving a gum which was taken up in ether and washed three times with 10% sodium hydroxide solution and with water before drying over magnesium sulphate. Removal of drying agent and solvent gave a viscous liquid having absorptions in the IR (film) at 2100, 2220, 3290cm This liquid (20.91 g) was heated in o-dichlorobenzene (40ml) at reflux temperature for 1.5 hours under nitrogen. After distillation of the solvent the fraction boiling at 110-114 / 0.02 mmHg (16.57 g) was collected, which on standing formed a low melting solid, having an IR absorption at 2230cm-1 (See M. Harfenist and E. Thom, J. Org. Chem., 37, 841 (1972) who quote m.p. 36-37 ). Addition to this 6-cyanochromene (16.50 g) dissolved in dimethyl sulphoxide (150ml) containing water (3.24 ml), of N-bromosuccinimide (31.90 g) with vigorous stirring and cooling, followed by dilution with water and extraction via ethyl acetate gave a mixture which was boiled in acetone (300ml) and water (100m1) for 5 hours to hydrolyse the small amount of 3,4-dibromide present. Evaporation of solvents gave 6-cvano-trans-5-bromo-5.4-dihYdro-2,2-dimethyl-2H-banzo- [b]pyran-4-ol as white crystals (24.37 g). A small sample had m.p. 128-128.5 from 60-80 petroleum ether, nmr (CDCl3) 1.43 (3H), 1.69 (3H), 7.48 (1H, exchangeable) 4.07 (1H, d, J=9), 4.87 (1H, d, J=9), 6.80 (1H, d, J=8), 7.43 (1H, 9, J=8, 2), 7.78 (1H, d, J=2). Anal. Calcd. for C12H12NO2Br: C, 51.07; H, 4.26; N, 4.96; Br, 28.37. Found: C, 50.95; H, 4.38; N, fOS; Br, 28.39%. The bromohydrin (24.30 g) was stirred with sodium hydroxide pellets (5.00 g) in water (250ml) and dioxan (200ml) for 3 hours at room temperature. The solvents were removed by distillation under high vacuum and the residue taken up in ether arid washed with water and brine before drying over magnesium sulphate. Removal of drying agent and solvent and gave crude 6-cyano-3,4-dihydro-2,2-dimethyl-3,4-epoxy 2H-benzo[b]pyran (16.02 g) as a gum, having an absorption at 2230 cm in the IR and Nmr (CCl4) i1.26 (3H), 1.54 (3H), 3.40 and 3.80 (each 1H, d, J=4), 6.77 (1H, d, J=8), 7.43 (1H, q, J=8, 2), 7.58 (1H, d, J=2). This epoxide (16.00 g) and pyrrolidine (7.20- ml) were refluxed in ethanol (240ml) for 3.5 hours. Removal of solvent, addition of ethyl acetate,and washing with water was followed by extraction with 5N hydrochloric acid. The acidic extract was basified with 10N sodium hydroxide solution and extraction via ethyl acetate gave a gum which was taken up in dimethyl ether containing a little ethanol and treated with ethereal hydrogen chloride. The precipi tate was collected and washed with diethyl ether leaving 6-cvano-5 4-dihvdro-2 ' 2-dimethvl-trans-4-tvrrolidino-2H-benzo [b]pyran-3-ol hydrochloride as a white solid (11.02 g), m.p. 202-204 ; IR absorption at 2220 cm-1; nmr (CDCl3) # 1.19 (3H). 1.73 (3H), 2.22 (4H, broad m), 3.13 (2H, broad m), 3.97 (2H, broad m), 4.20 (1H, d, J=8), 4.86 (1H, d, J=8), 5.53 (s, 1 exchangeable H, broad), 6.87 (IN, d, (J=8), 7,47 (1H, q, J=8, 2), 8.72 (1H, d, J=2). Anal. Calcd. for C16H21N2O2Cl: C, 62.23; H, 5.86; N, 9.07; C1, 11.48. Found: C, 62.34; H, 6.73; N, 8.82; Cl, 11.40%. Example 2 6-Cvano-3,4-dihydro-2,2-d'imethyl-trans-4-pyrrolidino-2H- benzoLb]pyran-3-yl 2,2-dimethylpropanoate To a solution of 4-dimethylaminopyridine (0.98 g) in dichloromethane (50 ml) was added 2,2dimethylpropanoyl chloride (0.69 ml) dropwise and with gentle stirring, followed by crude yano-3,4-dihvdro 2-dimethyl-trans-4-nyrrolidino-2H-benzo[blpyran-3-ol (2.50 g) in dichloromethane (50 ml) during 4 minutes. The resulting red solution was heated under reflux for 40 hours before cooling and evaporation of solvent. The orange residue was taken up in ethyl acetate and washed with water, dried and evaporated leaving a mustard coloured solid (2.97 g) which was separated by chromatography on silica gel (110 g) with mixtures of ethyl acetate and 60-80 petroleum ether using a gradient elution technique, into crude ester (1.12 g) and starting material (1.13 g). Recrystallisation of the crude material from 60-800 petroleum ether gave 6-cvano-3,4-dihvdro-2,2dimethyl-trans-4-pyrrolidino-2H-benzo[b]pyran-3-yl 2 2-dimethylpropanoate (0.73 g) as white crystals of m.p. 109-1100C; IR absorptions at 1730, 2220cm-1 nmr (CDC13) 6 1.24 (9H), 1.29 (3H), 1.41 (3H), 1.75 (4H, broad m), 2.73 (4K, broad m), 4.09 (1H, d, J=9), 5.33 (1H, d, J=9), 6.86 (1H, d, J=8) , 7.43 (1H, q, J=8, 2), 7.74 (1H, d, J=2). Anal. Calcd. for C21H28N2O3 : C, 70.76; H, 7.92; N, 7.86. Found: C, 70.74; H, 8.03; N, 7.76%. Example 3 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-3-methoxy-4pyrrolidino-2H-benzo[b]pyran To potassium t-butoxide (1.03 g) in dry toluene (40 ml) was added dropwise with stirring 6-cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrolidino-2H benzo[b}tvran-3-ol (2.50 g) in toluene (100 ml) under nitrogen. After 10 minutes, methyl iodide (0.62 ml) in toluene (20 ml) was added dropwise, and the resulting yellow reaction mixture was stirred at 75-800C for 16 hours. Cooling, and cautious addition of water, separation of the organic layer, washing with water, drying and evaporation gave a red gum (2.66g) which was separated, by column chromatography on silica gel (110 g) with mixtures of ethyl acetate and 60-80 petroleum ether using a gradient elution technique, into crude ether (1.34 g) and starting material (0.91 g). One crystallisation from 60-80 petroleum ether gave 6-cyano-3,4-dihydro-2,2-dimethyl-trans-3-methoxy-4 vrrolidino-2H-benzo[b]vran (1.06g) as off-white crystals m.p. 108-109 C; nmr (CDCl3) 6 1.22 (3H), 1.50 (3H), 1,80 (4H, broad m) , 2.76 (4H, broad m), 3.39 (1H, d, J=9), 3.51 (3H), 3.98 (1H, d, J=9), 6.76 (1H, d, J=8), 7.34 (1H, q, J=8, 2), 7.72 (1H, d, J=2). Anal. Calcd. for C17R22N202 : C, 71.30; H, 7.74; N, 9.78. Found: C, 71.12; H, 7.96; N, 9.72%. Example 4 Resolution of 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4pyrrolidino-2H-benzo-[b]-pyran-3-ol. Racemic 6-cyano-3,4-dihydro-2,2-dimethyltrans-4-pyrrolidino-2H-benzo[b]-pyran-3-ol (4.12 g) and (+)-Tartaric acid (2.55 g) both dissolved in ethanol, were combined and the resulting solution evaporated leaving a cream foam. Three recrystallisatitns from ethanol gave a tartrate (1.01 g) of mp 173-173.5 and an [a] = 64o. Basification with NaHCO3 and extraction with diethyl ether gave the minus isomer of the free base (0.64 g) of [&alpha;]EtOH = 1010. Treatment of an ethereal solution of this free base with ethereal- anhydrous HC1 and one recrystallisation from ethanol-diethyl ether gave (-)-6-cyano-3, 4dihydro-2,2-dimethyl-trans-4-pyrrolidino-2H-benzo-[b] pvran-3-ol hvdrochloride (0.49 g) of mp 184-185 C, [a] = Anal. Calcd. for C16H21N2 2C1: C, 62.23; H, 6.85; N, 9.07; Ci, 11.48. Found : c, 62.29; H, 7.11; N, 9.17; Ci, 11.39%. The mother liquors remaining after the three recrystallisations of the tartrate prepared from the racemic free base and (+)-tartaric acid were evaporated to dryness, dissolved in water, and basified with NaHCO3. Extraction via diethyl ether gave crude free base (3.10 g) which was treated with (-)-tartaric acid (1.67 g) in ethanol. Evaporation gave a cream foam (4.78 g). Four recrystallisations from ethanol gave a tartrate (1.42 g) of mp 172.5-173.5 and [a] +58 . Basification with NaHCO5 and extraction with diethyl ether gave the plus isomer of the free base (0.88 g) of [&alpha;]EtOH = +115 . Treatment of an ethereal solution of this free base with ethereal-anhydrous HC1 and one recrystallisation from ethanol-diethyl ether gave (+)-6-cvano-3,4-dihvdro- ¯.2-dimethYl-trans-4-pyrrolidino-2H-benzo-[b3-pyran-5-ol hydrochloride (0.87 g) of mp 175-177 C, [a] = +100 . Anal, Calcd, for C16H21N2O2C1 : C, 62.23; H, 6.85; N, 9.07; C1, 11.48. Found: C, 61.94; H, 7.06; N, 9.28; C1, 11.63%. (Optical rotations were determined in water unless otherwise stated and measured at the sodium-D line). Example 5 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrolidino-2E- benzo-[b]-pyran-3-ol hydrochloride 6-Cyano-trans-3-bromo-3,4-dihydro-2,2-dimethyl 2H-benzo[b]pyran-4-ol. (1.57 g) was dissolved in pyrrolidine (2.0 ml) and the solution heated under reflux for 25 min. After cooling, the solution was subjected to reduced pressure to remove traces of pyrrolidine. The residual gum was dissolved in ethyl acetate and washed with aqueous sodium carbonate solution, and water, before extraction with 1N hydrochloric acid. The organic layer was discarded, and the aqueous layer was basified with 2.5 N sodium hydroxide and extracted with ethyl acetate. Water washing, drying, and evaporation of the organic layer gave the crude 6-cvano-3,4-dihydro- 2,2-dimethyl-trans-4-pyrrolidino-2H-benzo[b]pyran-3-ol (0.85 g) having identical chromatographic characteristics to that described in Example 1. The hydrochloride salt was then prepared in the same manner as described in Example 1. DEMONSTRATION 1 - Effectiveness of the Compound of Example 1 Systolic blood pressures were recorded by 2 modlfi- cation of the tail cuff method described by I.M. Claxton, M.G. Palfreyman, R.H. Poyser and R.L. Whiting, European Journal of Pharmacology, 37, 179 (1976). An oscilloscope or W+W BP recorder, model 8002, was used to display pulses. Prior to all measurements rats were placed in a heated environment (33.5' 0.50C) before transfer to a restraining cage. Each determination of blood pressure was the mean of at least 6 readings. Spontaneously hypertensive rats (ages 12-18 weeks) with systolic blood pressures > 170 mmHg were considered hypertensive. Time Post % change % change Compound of dose in Systolic in heart Example 1 hours Blood rate Pressure 5 Rats Dose 1 mg/kg p.o. 1 -22; 2 +10 + 3 Initial Blood Pressure 208 + 3 2 -21+ 2 + 8 + 2 Initial Heart Rate 471+ 11 4 -29+ 2 + 7 + 2 6 -21+ 2 + 7 + 2 24 -18+ 2 - 4 #4 6 Rats Dose 0.3 mg/kg p.o 1 -21+ 2 + 2 + 1 Initial Blood Pressure 197+ 2 2 -12+ 2 - 8 # 2 Initial Heart Rate 477+ 5 4 - 8+ 2 - 6 + 1 6 -2#2 -4#1 24 + 6+ 4 - + L DEMONSTRATION 2 - Effectiveness of the Compound of Example 4 Systolic blood pressures were recorded by a modification of the tail cuff method described by I.M. Claxton, M.G. Palfreyman, R.H. Poyser and R.L. Whiting, European Journal of Pharmacology, 37, 179 (1976). A W and W B.P recorder, model 8002, was used to display pulses. Prior to all measurements, DOCA-Salt treated hypertensive rats were placed in a heated environment (33.5 # 0.5 C) before transfer to a restraining cage Each determination of b.p. was the mean of at least 6 readings. Time Post % chanae % chanae (-)-Isome dose in Svstolic in heart hours B.P. rate 5 rats Initial values 185 r 88 mmHg 344 + 17 beats/ min. 1 mg/kg p.o. 1 -3 + 3 16 + 7 2 -4 + 3 10 ; 7 4 -5 + 3 2 + 6 6 -1 # 2 2 # 2 24 9 + 1 17 + 6 Time Post % change % change (+)-Isomer dose in Systolic in heart hours B.P. rate 6 rats Initial values 191 # 4 mmHg 331 # 13 beats/ min. 1 mg/kg p.o. 1 -37 + 2 22 + 3 2 -27 + 3 17 l. 5 4 -23 + 4 7 + 6 6 -14 + 3 13 + 4 24 4 + 3 9 + 3

Claims: WHAT WE CLAIM IS: 1. A compound of the formula (I): EMI16.1 wherein the pyrrolid no and OR moieties are trans and wherein R is a hydrogen atom an alkyl group of 1 to 3 carbon atoms or an acyl group of 1 to 8 carbon atoms; or a pharmaceutically acceptable acid addition salt thereof. 2. 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrolidine -2K-benzo[b]pyran-3-ol or a pharmaceutically acceptable acid addition salt thereof. 3. 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrolidino -2K-benzo [b]pyran-3-ol hydrochloride. . 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrolidino -2H-benzo[bgpyran-3-yl 2,2-dimethylpropanoate or a pharmaceutically acceptable acid addition salt thereof. 5. 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-3-methoxy-4 pyrrolidino-2H-benzotbgpyran or a pharmaceutically acceptable acid addition salt thereof 6. A compound as claimed in claim 1 having the stereo chemistry of (+)-6-cyano-3,4-dihydro-2,2-dimethyl-trans- 4-pyrrolidino-2H-benzopyran-3-ol. 7. (+)-6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-pyrrol dino- 2H-benzo [b ]pyran- 3-01 or a pharmaceutically acceptable salt thereof. 8. (+)-6-Cyano-3, A -dihydro-2,2-dimethyl-trans-4-pyrroli- dino-2K-benzo [b]pyran-3-ol hydrochloride. 9. A pharmaceutical composition comprising a compound as claimed in any of claims 1 to 8 and a pharmaceutically acceptable carrier. 10. A composition as claimed in claim 9 in the form of an orally administrable shaped unit dose form containing from 2 to 50 mg of said compound as claimed in any of claims 1 to 8. 11. The use of a compound as claimed in any of claims 1 to 9 as a vasodilatory anti-hypertensive agent. 12. A process for the preparation of a compound as claimed in claim which process comprises the reaction of the compound of the formula (II): EMI17.1 with pyrrolidine and thereafter option ally reacting the thus produced compound of the formula (I) wherein R is a hydrogen atom and thereafter optionally alkylating -or acylating said compounds, resolving any of said compounds and/or salifying any of the resulting compounds.

Inventors: EVANS, JOHN MORRIS
Applicants: BEECHAM GROUP PLC
Application year: 1979
Main classification: C07D311
IPC codes: C07D311