PROCESS FOR PRODUCING ALKYL 4-(ALKOXY METHYL PHOSPHINOYL)BUTYRATES AND ALKYL-(2-AMINO-4-(ALKOXY METHYL PHOSPHINOYL)) BUTYRATES

Abstract: Dialkyl 4-methylphosphinobutyrates represented by the following general formula; (wherein R₁ represents hydrogen atom or acylamino group, and R₂ and R₅ each represents C₁-C₅ alkyl group) are inter­ mediates for D,L-2-amino-4-methylphosphinobutyric acid useful as a herbicide, and are prepared by reacting a corres­ ponding vinylacetic acid ester with methylphosphorous acid monoalkyl ester at 100-200°C in the presence of a radical-­ generating agent or under irradiation with ultraviolet rays.

Description: Title of tlic invention Organic phosphorus compounds and process for preparation thereof. Scope of the invention The present invention relates to 4-methylphosphinobutyric acid dialkyl esters and 2-substituted amino derivatives thereof which are intcrmcdiates useful for synthesis of herbicidal and anti-microbial DL-2-amino-4-methylphosphinobutyric acid, and to a process of producing the same. Background of the invention Several processes of producing DL-2-amino-4-methylphosphinobutyric acid have already been proposed. The examples of these processes include a process of producing said compound through p-chloroethyl-methyl-phosphinic acid p-chlorocthyl ester, described by H. Gross et al (J. Pract. Chem. 318, p. 157, 1976), a process of producing the same from dialkyl methylphosphonite and 3-halopropanal dialkylacetal, by P. Mastalerz et al (Rocz. Chem. 9, p. 2127, 1975). Furthermore the present inventors have also proposed a process using a reaction between a 2-haloethyl-methyl-phosphinic acid ester and acetamidomalonic acid ester (Japanese Preliminary Patent Publication No. 48-91019). Disclosure of the invention The present inventors have made extensive research for estab -lishing a method to economically produce DL-2-amino-4-methylphos- phinobutyric acid through intermediates different from and more advantageous than that of the above mentioned prior arts because of cheaper materials. As a result of the research, it has been found that 4-methyl- phosphinobutyric acid dialkyl ester and 2-substituted amino--methyl- pbospllinobutyric acid dialkyl ester which are derived from vinyla- cetic acid ester aiid -substituted amino-vinylacetic acid ester respectively by phosphorylation with methylphosphorous nonoalkZl ester, and that said dialkyl esters can be effectively used for synthesis of DL-2-amino-4-methylphosphinobutyric acid by way of subsequent two independent pathways. Tile present invention is advantageous industrially in that vinylacetic acid ester as the material for 4-methylpIiospiinobutyric' acid dialkyl ester is inexpensive and that through 2-substituted -amino-4-methylphosphinobutyric acid dialkyl ester, the consumption of expensive phosphorous agent is limited to a minimum. The present invention will be described in more detail. As a starting material, there is used vinylacetic acid ester or an -substituted-amino-vinylacetic acid ester represented by the general formula (1) EMI2.1 wherein R1 represents hydrogen atom, an acylamino group expressed by R3CONE-(Where R3 is a Cl-C5 lower alkyl group, trifluoromethyl 15 group, trichloromethyl group, monochloromethyl group or phenyl group), a phthalimino group, or an N-acyl-N-benzylamino group ex- pressed by EMI2.2 (where R4 is an acetyl group, trifluoroacetyl group or benzoyl group), and R2 represents a C1-C5 lower alkyl group (straight or branched). The ester of N-acyl-vinylglycine which is one of the representatives of -substituted amino-vinylacetic acid esters of the formula (l) in which R1 is not hydrogen is obtained by treating vinyiglycine (J.E. Baldwin et al, J. Org. Chem. 42, 1239, 1977) with an acid anhydride such as acetic anhydride, propionic anhydride or trifluoroacetic anhydride, or with an acid chloride such as acetyl chloride or benzoyl chloride and esterifying the N-acyl compound obtained. The ester of N-benzyl-N-acyl-vinylglycine is obtained by reacting an oc-bromo-vinylacetic acid ester with benzylamino in an organic solvent such as dimethylformamide, benzene, toluene, or dimethy lsulfoxide and subjecting the resulting N-benzyl-vinylglycine ester to N-acylation with acetic anhydride or trifluoroacetic anhydride. N-phthaloyl-vinylglycine ester is obtained by reacting > -bromo -vinylacetic acid ester with potassium phtalimide in a solvent such as dimethylformamide or dimethylsulfoxide. The thus obtained vinylacetic acid ester or > -substituted -amino-vinylacetic acid ester is reacted with a methylphosphorous monoalkyl ester of the general formula (2) EMI3.1 wherein R5 represents a C1-C5 lower alkyl group (straight or bran ched), in the presence of a free radical-generating agent such as di-t-butyl peroxide, dibenzoyl peroxide, t-butyl peroxide, t-butyl hydroperoxide or acetylbenzoyl peroxide, or under ultraviolet-irradiating condition, usually in a solvent-free condition at a tempera ture ranging from 100 to 2000C for a reaction time- of 1 to 8 hours. This reaction can be also conducted in an organic solvent such as toluene and xylene. By this reaction, there is obtained a 4-methylphosphinobutyric acid dialkyl ester or a 2-substituted-amino-4-methylphosphinobutyris acid dialkyl ester as a product of this invention each expressed by the general formula (3) EMI3.2 wherein Rl R2 and R5 have each the same meaning as defined above. The herbicidal DL-2-amino-4-methylphosphinobutyric acid can be prepared by subsequent different processes corresponding to the substituent R1, as described below. The resulting 4-methylphosphinobutyric acid dialkyl ester of the formula (3) in which the R1 is hydrogen is subjected to a hydrolysis reaction under an appropriate condition with an acid such as hydrochloric acid, sulfuric acid, hydrobromic acid or trifluoroacetic acid or an alkaline solution such as of sodium hydroxide, potassium hydroxide or barium hydroxide, to effect deesterification of the carboxylic ester, thereby giving 4-methylphosphinobutyric acid or 4-methylphosphinobutyric acid monoester of the general formula (4) EMI3.3 wherein R represents hydrogen atom or a lower alkyl of C -c 5 15 atoms (straight or branched). For instance, when LI-methylphosphinobutyric acid dialkyl ester is located in 0.1 to 2 N sodium hydroxide solution in water, aqueous ethyl alcohol or aqueous acetone, at 40 to 800C for 1 to 5 hours, there is obtained 4-methylp}losphinobutyric acid. Wlen 4-methylphosphinobutyric acid dialkyl ester is heated in aueous 0.5 to iN hydrochloric acid at a temperature of 50 to 70 C for 1 to 2 hours, the carboxylic acid ester alone is deesterified, there is obtained 4-methylphosphinobutyric acid monoester. Particularly, in the case of t-butyl ester of the carboxylic acid, the selective deesterification proceeds easily even at a room temperature. The compound of the general formula (4) is converted to 2-halogenocarboxylic acid of the general formula (5) EMI4.1 wherein R5 ; has the same meaning as defined above, and X represents a halogen atom, by the use of the halogenation reaction at the cc-position of the carboxylic acid, as described below. For example, the halogenation reaction is easily conducted either by subjecting the compound of the formula (4) to a bromination with bromine or a chlorination with sulfuryl chloride in the presence of a compound such as phosphorus trichloride, phosphorus tribromide or red phosphorus, or by converting the compound of the formula (4) to its acid chloride by treating with thionyl chloride, followed by chlorination by the reaction with chlorins gas in the presence of iodine, red phosphorus or an organic peroxide under ultraviolet-irradiating conditions. Some of the reagents mentioned may induce not only the halo genation, but also conversion of the carboxylic acid moiety into the acid halode. In such a case, however, the treatment with cold water is sufficient to convert the acid hay ode to the original free carboxylic acid. The thus obtained -halide of the general formula (5) is then treated with a usually employable aminating agent such as concentrated aqueous ammonia or liquid ammonia to obtain DL-2-amino-4 methlphosphinobutyric acid as the final compound. Alternatively the compound of the general formula (5) may be reacted with sodium nitride or hydrazine to form an & azide compound or an d-hydrazine compound which is subsequently reduced in a known manner to obtain the DG2-amino-4-methylphosphinobutyric acid. 2-substituted-amino-4-methylphosphinobutyric acid dialkyl ester of the formula (3) in which the R1 is other than hydrogen atom is hydrolyzed by the use of an inorganic acid such as hydrochloric acid, sulfuric acid or hydrobromic acid or of an alkaline solution containing sodium hydroxide, potassium hydroxide or barium hydroxide to obtain an intended compound of DL-2-amino-4methylphosphinobutyric acid The final compound can be separated from the reaction solution advantageously by an ion exchange chromatographic technique or a crystallization technique, by which pure DL-2-amino-4-methylphosphinobutyric acid can readily be obtained. Preferred embodiments of the invention. The present invention is nov illustrated with reference to the following Examples to which the present invention is not limited in any way. Example l 4.08g of methyl phosphorous monoisobutyl ester was placed in a 20ml flask, into which 3.42g of vinylacetic acid ethyl ester containing O.lg of t-butyl peroxide was dropped over 3 hours while agitating at 145 to 1500C. After reaction for further 1 hour at the temperature, the reaction product was dissolved in 20ml of chloroform, and charged into a silica gel column with a volume of 70ml, followed by eluting it with a mixed solvent of chloroform and methanol (20:1). The resulting eluate was concentrated to obtain 7.lg of an oil substance of 4-(0-isobutyl-methylphosDhino)butyric acid ethyl ester. Elementary analysis Found : C 52.59, H 9.27% Calculated for C11H2304P: C 52.80, H 9.20, 0 25.60, P 12.40 EMI6.1 Example 2 6 .log of 4-(O-isobutyl-methylphosphino)butyric acid ethyl ester obtained in the Example 1 was dissolved in 40ml of aqueous IN sodium hydroxide solution and heated for 3 hours at. The resulting reaction product was adjusted to pH 2.0 by addition of an aqueous 5N hydrochloric acid solution, and concer.t- rated and evaporated to dryness as it is. The thus obtained residue was extracted with 30ml of ethanol and the extract was evaporated to dryness to obtain 3.4g of a syrup of 4-methylphosphinobutyric acid. Elementary analysis Found 36.08, H 6.54% Calculated for C5Hll04P: C 36.14, H 6.63, 0 38.55 P 18.67 To 3.3g of the thus obtained 4-methylphosphinobutyric acid, was added I.76g of bromine, to which was further 0.04ml of phosphorus trichloride, followed by reacting at 700C for 15 hours under agitation. The reaction product was evaporated to dryness to obtain 4.2g of a crude syrup of 4-methylphosphino-2-bromo-butyric acid. The thus obtained substance was then dissolved, without purification, in 30ml of 28 aqueous ammonia and allowed to stand at room temperature for 6 days. The reaction product was evaporated to dryness to remove excess ammonia therefrom and then dissolved in 5ml of water. The solution was adjusted to pH 2 with aqueous 5N hydrochloric acid solution and evaporated to dryness. The residue was extracted with 20ml of ethanol and the extract was subjected to distillation to remove ethanol therefrom. The residue was subjected to column chromatography with 50ml of Dowex 1 x 2 (acetic acid type) which was developed with an aqueous 3N acetic acid solution, to collect a ninhydrin positive fraction, followed by concentrating and evaporating to dryness to obtain l.4g of DL-2-amino¯4-methylphos¯ phinobutyric acid. Example 3 5.5g of the 4-(O-isobutyl-methylphosphino)butyric acid ethyl ester obtained according to Example 1 was suspended in 80ml of an aqueous 1N hydrochloric acid solution and heated at 700C for 2 hours under agitation. The reaction solution was evaporated to dryness and dried over sodium hydroxide in high vacuum to obtain 4.6g of 4-(0-isobutyl fnethylphosphino) butyric acid. This substance was found from the NMR spectrum-that only the absorptions of 4-(O-isobutyl- methylphosphino)butyric acid ethyl ester at 1.25 (t) and 4.18(g) ppm disappeared. Elementary analysis Found: C 48.52, H 8.63% Calculated for C9H1904P: C 48.65, H 8,65, 0 28.83. P 13.96% Then, 20ml of thionyl chloride was added to 4.45g or the substance for reaction at 50 to 600C for 2 hours. with agitation. To the thionyl chloride solution of the acid chloride obtained was added 0.25g of iodine, followed by violently agitating at 85 to 900C under ultraviolet irradiation w-hile passing chlorine gas. The reaction time was 6 hours. The thionyl chloride was removed from the reaction solution by distillation and lOml of iced water was added to the residue to decompose the acid chloride. The solution was again evaporated to dryness, followed by drying over sodium hydroxide in high vacuum to obtain 4.72g of a syrup of 2-chloro-4-(O-isobutyl-methylphosphino)butyric acid. 300 mg of the resulting syrup was dissolved in 0.5ml of methanol and was subjected to a silica gel column (40ml) which was then eluted with CHCl3 -CH3OH (5:1) to obtain 180mg of sample for analysis. Elementary analysis Found: C 41.82, H 7.24, C1 12.68% Calculated for C9H18O4Cl P: C 42.10, H 7.02, 0 24.95, C1 13.84 P 12.08% EMI7.1 The remainder of the syrup was dissolved in 40m1 of 28% aqueous ammonia and allowed to stand for 7 days at room temperature, and heated for 4 hours at 600C. The reaction solution was evaporated to dryness and then treated in the same procedue as in example 2 to obtain l.lg of DL-2-amino-4-methylphosphinobutyric acid. Example 4 1.2g of vinylglycine was dissolved in lOnil of trifluoroacetic acid, to which was added 2.4ml of trifluoroacetic anhydride at 0 C. After being agitated for 30 minutes, the solution was allowed to stand for 3 hours. The reaction solution was evaporated to dryness and the residue was dissolved in lOml of methanol, to which was added 50ml of an ether solution of diazomethane at O to 5 C, followed by allowing to stand at 5 C for 1 hour. The reaction solution was evaporated to dryness to obtain 2.3g of an oily substance of methyl NT-trifluoroacetyl-vinylglycinate. Then, 1.36g of methylphosphorous monoisobutyl ester was added to 2.lg of the oily substance obtained, followed by agitating at 150 C for 5 hours, while di-t-butyl peroxide, divided into 10 portions, was added portion by portion in a total amount of 0.2g. The reaction solution was evaporated to dryness and 3.43g of the product containing 4 - (0-is obutyl-methylphospbino) -2-trirThoroaoetyl-arnum butyric acid methyl ester was obtained. 200mg of the product was dissolved in 0.5ml of chloroform and subjected to a silica gel column (30ml) which was then eluted with chloroform-methanol (40:1) to obtain 85mg oily sample to be analyzed. Elementary analysis Found: C 41.25, H 6.18, N 3.92% Calculated for C12H21 0 TPF 5 3 C 41.50, H 6.05, 0 23.05, N 4.03, P 8.93, F 16.43% EMI8.1 The remainder of the produCt was dissolved in 20ml of an aqueous 5N hydrochloric acid and hydrolyzed at lO00C for 4 hours. The hydrolyzed solution was evaporated to dryness and the residue was dissolved in lOml of water and discolored with use of carbon powder, after which it was adjusted to pH 3 with aqueous 3N sodium hydroxide solution. The thus pH adjusted solution was concerntrated to about 3ml to which was added 8ml of ethanol, followed by allowing to stand at low temperature so as to effect crystallization. The crystals were collected by filtration to obtain 1.35g of white crystals of DL-2-amino-4-methylphosphinobutyric acid. This substance was found to exhibit the same melting point as the control substance without showing any depression of melting point. Example 5 1.8g of vinylglycine was dissolved in 9ml of water, into which was dropped 6m1 of acetic anhydride under ice-cooloing over 2 hours with agitation. The solution was agitated at 5 to 10 C for further 5 hours and then evaporated to dryness, thereby obtaining crystals. The crystals were suspended in 5ml of acetone and then separated by filtration to obtain 2.lg of white crystals of N-acetylvinylglycine having a melting point of 118 to 119 C. 2.0g of the crystals were dissolved in 80ml of methanol to which was added 0.3ml of concentrated sulfuric acid, followed by refluxing'for 4 hours under heating conditions. The reaction solution was allowed to cool, neutralized with sodium hydrogen carbonate, and evaporated to dryness. The residue was dissolved in 50ml of chloroform and washed lOml of a saturated saline solution. The chloroform layer was dehydrated with anhydrous sodium sulfate and evaporated to dryness to give 2.2g of an oily substance of methylN-acetyl-vinylglycinate. To 1.6g of the thus obtained methyl-N-acetyl-vinylqlrcinate was added 1.35g-of methyl phosphorous monoisobutyl ester, to which was further added portion by portion O.15g of di-t-butyl poroxide, divided into ten portions, while agitating at 1600C for 4 hours. The reaction solution was evaporated to dryness to obtain 2.68g of the product containing 4-(0-isobutal) methylphosphino-2 N-acetylbutyric acid methyl ester. I To the product was added 25ml of aqueous 6N hydrochloric acid solution to hydrolyze at l100C for 20 hours. Then, the procedure of Example 4 was repeated to obtain l.12g of crystals of DL-2-amino -4-methylphosphinobutyric acid. Example 6 2.5g of ethyl & bromo-vinylacetate was dissolved in lOml of dimethyl:formamide, to which was added 2.38g of patassium phthalimidc, followed by agitating at room temperature for 6 hours. The reaction solution was diluted by addetion of looms of chloroform and the insoluble matter was removed by filtration. The resulting filtrate was washed twice with 30ml of a saturated saline solution. The chloroform layer was treated with anhydrous sodium salfate to remove water therefrom, concentrated to about 5ml, and allowed to stand at a low temperature, whereupon crystals precipitated in small amount. The crystals were removed by filtration and the filtrate was evaporated to dryness to obtain an oily substance. This substance was dissolved in l0ml of ethyl ether. WEen the solution was allowed to stand at a low temperature, crystals appeared. The crystals were collected by filtration to obtain 3.5g of white crystals of ethyl N-phthaloyl-vinylglycinate with a melting point of 95 to 97 C. To 1.29g of the crystals of ethyl N-phthaloylvinylglycinate was added 0.68g of methylphosphorous nonoisobutyl ester and diluted with 3ml of xnlene. O.lg of di-t-butyl peroxide which had been divided into ten portions was added portion by portion to the mixture at 1400C over 6 hours under agitation. The reaction solution vas evaporated to obtain 1.46g of dried product containing 4- (O-isobutyl -nethylhosp;iino) -2-N-phthaiiitiido- butyric acid ethyl ester. The product was dissolved in 5m1 of aqueous 40% sulfuric acid and refluxed for 4 hours to effect hydrolysis. The reaction solution was decolorized with activated carbon powder and allowed to stand at low temperature, followed by separating precipitated crystals of phthalic acid by filtration. The obtained filtrate was neutralized with barium carbonate to obtain barium sulfate which was filtered off. The filtrate was treated in the similar manner as in Example 4 to obtain 0.3g of crystalls of DL-2-amino-4-methylphosphinobutyric acid.

Claims: What is claimed is: v v r t 1. 4methylphosphinobu'tyric acid dialkyl ester of 2 substituted amino-4-methylphosp}linobutyric acid dialkyl ester represented by the general formula: EMI11.1 wherein R1 represents hydrogen atom, an acylamino group expressed by R3CONE-(wherein R is a C -C lower alkyl group 3 15 trifluoromethyl group, monochloromethyl group or phenyl group) a phthalimino group, or an N-acyl-N-benzylamino group expressed by EMI11.2 (wherein R4 is an acetyl group, triflu oroacetyl group or benzoyl group), and R2 represents a C1-C5 lower alkyl group. 2. A process for production of 4-methylphosphinobutyric acid dialkyl ester of 2-substituted amino-4-methylphosphinobutyric acid dialkyl ester represented by the general formula: EMI11.3 wherein R1 represents hydrogen atom, an acylamino group expressed by R3CONH-(wherein R3 is a C1-C5 lower alkyl group, trifluoromethyl group, trichloromethyl group, monochloromethyl group or phenyl group) a phthalimino group, or an N-acyl-Nbenzylamino group expressed by EMI11.4 (wherein R4 is an acetyl group, trifluoroacetyl group or benzoyl group), and R2 represents a C -C lower alkyl group, which comprises reacting viiiylacetic acid ester or -substituted aminovinylacetic acid ester of the general formula: : EMI11.5 wherein R1 and R2 have each the same meaning as defined above, with a methylphosphorous monoalkyl ester of the general formula: EMI11.6 wherein R5 has the same meaning as defined above, in the presence of a free radical-generating agent or under ultraviolet-irradiating condition, at a temperature from 100 to 2000C.

Inventors: INOUYE, SHIGEHARU, MIZUTANI, KAZUKO, SEKIZAWA, YASUHARU, SUZUKI, AKIRA, TACHIBANA, KUNITAKA, TAKEMATSU, TETSUO, TSURUOKA, TAKASHI
Applicants: MEIJI SEIKA KAISHA LTD.
Application year: 1979
Main classification: C07F9
IPC codes: C07F9