1.2.4-TRIAZOLIDINE-3.5-DIONE DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

Abstract: Coumpounds of the formula (I) wherein: n is 1 to 5 Y is -CH₂-CH₂, -CH=CH- or -C≡C- R₁ is hydrogen or CO₂R₁ represents an ester group in which the R₁ moiety contains from 1-12 carbon atoms; R₂ is hydrogen, C₁₋₄ alkyl or phenyl; R₃ is hydroxy or protected hydroxy; R₄ is a group of the formula: L-Q-R₁₀ in which Q is an oxygen atom, a -CH=CH- group or a -C≡C- group, and L is a C₁₋₉ alkylene group; and R₁₀ is C₁₋₄ alkyl, phenyl or naphthyl, any of which phenyl or naphthyl moieties may be substituted by one or more halogen, trifluoromethyl, C₁₋₆ alkyl, hydroxy, C₁₋₆ alkoxy, phenyl C₁₋₆ alkoxy or nitro groups, or if Q is a -CH=CH- group, the R₁₀ may be a hydrogen atom; and R₅ is hydrogen or C₁₋₆ alkyl; and salts thereof, having similar activity to natural prostaglandins, a process for their preparation, intermediates useful in that pro­ cess and pharmaceutical compositions containing them.

Description: Novel Organic Compounds This invention relates to novel compounds having pharmacological activity, to a process for their preparation, to intermediates useful in that process. and to pharmaceutical composition containing them Offenlegungsschrift No: 2323193 discloses that pyrazolidine derivatives of the formula (I)': wherein: EMI1.1 A is CH=CH or CHIC; R is H, an alkali metal, an amine salt, or a # 12C hydrocarbon or chlorohydrocarbon residue; m is Oor 1; n is 0-6; p is 0-6; and Y and Z are 0 or H except that Y and Z are not both 0; have similar biological properties to the prostaglandins or are antagonists of prostaglandins. French Patent Application No: 2258376 discloses that 10-aza prostaglandins of formula (II)": EMI2.1 wherein: R=H or lower alkyl; R' and R" = CH3 or C2H5; R =H or lower alkyl; Y = -CH2-CH2-, or -CH = CH-; Z = -CO or -CH(¯CH)-; are useful in the treatment of blood pressure and gastro-intestinal disorders, and in the preparation for confinement. Belgian Patent No: 835989 discloses that compounds of the formula (III)": EMI2.2 wherein: X is CO, protected CO, CROH in which R is hydrogen or C14 alkyl and in which the OH moiety may be protected; Y is CH2CH2 or CH=CH; Z is CO or CH2; n is 1 to 8; m is 1, 2 or 3; R1 is hydrogen, CH2OH, CH2OH in which the Oh moiety is protected, CO2W wherein W is hydrogen or Co2W represents an ester group in which the ester moiety contains from 1 to 12 carbon atoms, or CONH2; R2 is hydrogen, C1-4 alkyl, or taken together with R3 and the carbon atom t.o which it is attached represents a carbonyl group; R3 is hydrogen, hydroxy or protected hydroxy; R4 is hydrogen or C1 galkyl; and salts thereof; have useful pharmacological activity. A novel class of compounds also having useful pharmacological activity his now been discovered, which compounds are structurally distinct from the prior art referred to above. Accordingly the present invention provides a compound of the formula (I): EMI3.1 wherein: n is 1 to 5 Y is -CH2-CH2, -CH=CH- or -CC- R1 is hydrogen or C02R1 represents an ester group in which the R1 moiety contains from 1-12 carbon atoms; R2 is hydrogen, C1-4 alkyl or phenyl; R3 is hydroxy or protected hydroxy; R4 is a group of the formula: L - Q - R10 in which: Q is an oxygen atom, a -CH=CH- group or a -C¯C- group, and L is a r alkylene group; and R10 is C1-4 alkyl, phenyl or napnthyl, any of which phenyl or naphthyl moieties may be substituted by one or more halogen, trifluoromethyl, C1 6alkyl, hydroxy, C1-6alkoxy, phenyl C1 6alkoxy or nitro groups, or, if Q is a -CH=CH- group, then R10 may be a hydrogen atom; and R5 is hydrogen or C16alkyl; and salts thereof. Suitably n is 2, 3 or 4, preferably 3. Similarly Y may be -CH2-CH2- or -CH=CH-, suitably -CH2-CH2-. R1 is hydrogen, or C02R1 represents an ester group in which the R1 moiety contains from 1 to 12 carbon atoms. Examples of R1 include hydrogen, methyl, ethyl, n- and iso-propyl, sec- and tert-butyl, phenyl, benzyl, tolyl and the like, while normally hydrogen or C1-6 alkyl groups are preferred. Suitable examples of R2 include hydrogen, methyl, ethyl and phenyl. Preferred examples of R2 include methyl. Suitable protected hydroxy groups R3 include readily hydrolysable derivatives such as acylated hydroxy groups in which the acyl moiety contains 1 to 4 carbon atoms, for example the acetoxy group; and hydroxy groups etherified by inert groups such as benzyl or methyl. Preferably however R! i ]ydroxy. Within R4, L may be straight chain alkylene groups such as n-propylene, n-butylene , n-pentylene, n-hexylene and n-heptylene or may be alkylene groups branched by one or two methyl groups at the same or different carbon atoms. R4 groups more suitably have the structure -(CH2)J-Q-R10, optionally substituted by one or two methyl groups at the same or different non-R10 carbon atoms. Within these more suitable groups R4, when Q is an oxygen atom, j is 1 to 6. j is preferably 4 to 6, in particular 5, when R10 is CI4 alkyl. Within these more: suitable groups R4, when Q is a -CH=CHgroup or a -C#C- group, j is 1 to 4, preferably 1 or 2. When R10 is C14 alkyl or a hydrogen atom subject to the proviso given under formula (I), then R10 is preferably methyl or a hydrogen atom subject to the aforementioned proviso, so that specific examples of such R4 groups are -(CH2)5-O-Me and -(CH2)2-CH=CH2. When R10 in R4 is C1,4 alkyl, it may suitably be straight chain, such as methyl, ethyl, n-propyl and n-butyl, or may suitably be branched by one or two methyl groups at the same or different carbon atoms, such as iso-propyl or tert-butyl; it is preferably methyl or ethyl. When R4 contains an aryl moiety as previously defined, these groups may be substituted in the phenyl or naphthyl moiety by normally one, two or three groups selected from those substituent groups listed hereinbefore. Examples of suitable substituent groups include fluorine, chlorine and bromine atoms and CF3, methyl, ethyl, n- and iso-propyl, m'tIry and ethoxy, n- and iso-propoxy and nitro groups. Other examples of such groups include hydroxy and bezyloxy. Preferably the aryl moieties when substituted by such groups will be mono or di-substituted. Suitable examples of R5 include hydrogen, methyl, ethyl, n- and iso-propyl, and n-, sec and tert-butyl. Preferred R5 groups include methyl. The compounds of the formula (I) may form conventional salts when R1 is hydrogen and also when R5 is hydrogen. Such salts include those with alkali and alkaline earth metals, suitably sodium and potassium, and ammonium and substituted ammonium salts. Normally salts of the R5 hydrogen will be with alkali metals. One particularly suitable sub-group of compounds within formula (I) is of formula (II): EMI6.1 wherein: Y, R1 and R5 are as defined in formula (I); nÚ is 2, 3 or 4; RÚ2 is hydrogen, methyl, ethyl or phenyl; and RÚ4 is a group of the formula: L-O-RÚ10 in which L is a C1-9 alkylene group; and RÚ10 is C1-4 alkyl; and salts thereof. Suitably in formula (II) nÚ is 3. Also Y is preferably -CH2CH2-. Suitably R1 is hydrogen or C1-6 alkyl, preferably hydrogen. Suitably RÚ2 is hydrogen, methyl or ethyl, preferably methyl. Suitable and preferred straight chain and branched groups L include those previously described as suitable and preferred. Thus L is preferably a C4-8 alkylene group. Suitable and preferred RÚ10 are as so described for R10 C1-4 alkyl groups under formula (I). R5 is ofter methyl or ethyl, preferably methyl. From the aforesaid it will be realised that one preferred group within formula (II) is of formula (III): EMI7.1 wherein: R, is as defined in formula (I); R 2 is hydrogen, methyl or ethyl and 4 is a group of the formula: L1 - 0 - R210 in which L1 is a C4 8 alkylene group; and Rê10 is C1-2 alkyl; and salts thereof. Suitably R1 in formula (III) is hydrogen or C1-6 alkyl, preferably hydrogen. Suitable and preferred groups L1 include those listed hereinbefore for L. Another particul.trly suitable sub-group of compounds within formula (I) is of formula (IV): EMI8.1 wherein: Y, R1 and R5 are as defined in formula (I); nÚ is 2, 3 or 4; RÚ2 iE hydrogen, methyl, ethyl or phenyl;and R4 is a group of formula (V): EMI8.2 wherein: L is as defined in formula (II); and V, W and Z are each hydrogen or fluorine, chlorine or bromine atoms, or CF3, methyl, ethyl, n- or iso-propyl, methoxy, ethoxy, n- or iso-propoxy or nitro groups; and salts thereof. In formula (IV) it is preferred that n1 is 3. Suitably R1 is hydrogen, sodium or C16 alkyl more preferably hydrogen. V and W will often be hydrogen. Preferred groups L are often those groups described as preferred under formula (I) for L. Often R5 will be methyl or ethyl, preferably methyl. Another particularly suitable sub-group of compounds within formula (I) is of formula (VI): EMI9.1 wherein: Y, n1, R1, R12 and R5 are as defined in formula (II); and R44 is a group of the formula - L - Q1 ¯ R3 10 in which L is as defined in formula (I); a -CH=CH- group or a -C#C- group; is C1-4 alkyl, or if Q1 is a -CH=CH- group, then R 10 may be a hydrogen atom; and salts thereof. Suitably in formula (VI) n1 is 3. Suitably R1 is hydrogen or C16alkyl, preferably hydrogen. Suitably R12 is hydrogen, methyl or ethyl. Suitable and preferred straight chain and branched groups L include those previously described as suitable and preferred. Thus L is preferably C1-4alkylene. Suitable and preferred groups R 10 include hydrogen, methyl and ethyl. Often in formula (VI) R5 will be methyl or ethyl, preferably methyl. From the aforesaid it will be realised that one preferred group within formula (VI) is of formula (VII): wherein: EMI10.1 R1 is as defined in formula (I); R12 is hydrogen, methyl or ethyl; R54 is a group of the formula L -QÚ-R410 in which L is a C1-4 alkylene group; QÚ is a group -CH=CH- or a group -C#C-; R410 is C1-2 alkyl or may be hydrogen if QÚ is a -CH=CHgroup; and salts thereof. Suitably R1 in formula (IX) is hydrogen or C1-6 alkyl, preferably hydrogen. Suitable and preferred groups L3 include those listed hereinbefore for Another particularly suitable sub-group of compounds within formula (I) is of formula (VIII): EMI11.1 wherein: Y, R1 and R5 are as defined in formula (I); nÚ is 2, 3 or 4; RÚ2 is hydrogen, methyl, ethyl or phenyl; R64 is a group of formula (IX): EMI11.2 wherein: L and QÚ are as defined in formula (VI); and V, W and Z are each hydrogen or fluorine, chlorine or bromine atoms, or CFD, methyl, ethyl, n- or isopropoxy or nitro groups; and salts thereof. In formula (VIII) it is preferred that n1 is 3. Suitably R1 is hydrogen or C1-6 alkyl, more preferably hydrogen. Preferred L groups are as so described undet formula (I). Also V and W will often be hydrogen. Preferably R5 is a C1-6 alkyl group. Often R5 will be methyl or ethyl, preferably methyl. Process Variant a) The invention also provides a process for the preparation of a compound oi the formula (I), which process comprises reacting a compound of formula (X): EMI13.1 wherein: R2, R3, R4 and R5 are as defined in formula (I), with a compound of formula (XI): T-CH2-Y-(CH2)nCO2R1 (XI) wherein T is a good leaving group, and Y, n and R1 are as defined in formula (I). @ suitably a halogen, such as bromine, and the reaction suitably carried out in an organic solvent such as hexamethylphosphoramide or the like in the presence of a base such as sodium carbonate or the like. Generally it is preferred that R1 in the compound of formula (XI) is other than hydrogen, and so if in this case a R1 hydrogen compound of the formula (I) is desired it is prepared from the thus formed compound of the formula (I) by a convontional deesterification reaction. When R3 is a protected hydroxy group in the compound of formula (X), then if in this case a compound of the formula (I) wherein R3 is hydroxy is required it is prepared from the thus formed R3 protected hydroxy compound by conventional de-protection reactions. For example, when R3 is a benzyloxy group, the benzyl group may readily be removed by hydrogenolysis. Thus it can be seen that compounds of the formula (I) wherein R3 is protected hydroxy are useful intermediates in the preparation of the corresponding free hydroxy compounds of the formula (I). Process Variant b) The inven+ion also provides a preferred process for the preparation of a compound of the formula (I), which process comprises reacting a compound of the formula (XII): EMI14.1 wherein: Y, n, R1, R4 and R5 are as defined in formula (I); with a reducing agent to give a corresponding compound of the formula (I) wherein R2 is hydrogen and R3 is hydroxy, or with a C1 4alkyl or phenyl Grignard reagent or C1-4 alkyl or phenyl metallic complex to give a corresponding compound of the formula (I) wherein R2 is C14 alkyl or phenyl, and R3 is hydroxy; and then optionally protecting the R3 hydroxy moiety. The reduction of the side chain carbonyl in a compound of the formula (XII)may be carried out by conventional methods for reducing a ketone to an alcohol, for example by sodium borohydride reduction. The C14 alkyl or phenyl Grignard reagent or C14 alkyl or phenyl metallic (suitably C14 alkyl or phenyl lithium) complex reaction may be carried out under conventional conditions for sch reactions, for example in an inert anhydrous solvent. The optional protection of the R3 hydroxy moiety may be carried out in conventional manner, for example by acylating, alky'ating or benzylating the R3 hydroxy compound. Process Variant c) The invention also provides a further preferred process for the preparation of a compound of the formula (I), which process is convenient when R5 in the compound of formula (I) is not a hydrogen atom, and which comprises reacting a compound of formula (XIII): EMI15.1 wherein: Y, n, R1 and R5 are as defined in formula (I), with a co.pornd of formula (X (V): EMI15.2 as defined above. Suitable reaction conditions are those given hereinafter as suitable for the reaction of the compound of the formula (XIV) with the compound of the formula (XVII). Suitable and' preferred examples of D are those hereinafter described as suitable and preferred in the compounds of the formula (XIV). After these reactions if so desired the group R1 in the thus formed compounds of the formula (I) may be varied by conventional esterification and/or de-esterification reactions. Similarly when R1 and/or R5 is hydrogen in such compounds of the formula (I), salts of these compounds may be prepared in conventional manner, for example, by reacting the chosen compound of the formula (I) with the required base. Preferably strong bases such as sodium in an alcohol, e.g. ethanol, and similar reagents are used to obtain salts of R5=H compounds. When in the group R4 Q is 0, and Y is -C--C- or -CH=CH-. Y may be converted to the corresponding cis- CH=CH or CH2CH2 group by any usual reduction method such as palladium-catalysed hydrogenation. The preparation of the intermediates for use in the processess of the invention will now be described. Process Variant a), Intermediates Compounds of the formula (X) may be prepared by reacting n "'jnpound of the formula (XVI): EMI16.1 with a reducing agent to give a corresponding compound of the formula (X) wherein R2 is hydrogen and R3 is hydroxy. The reduction may be carried out as hereinbefore described with reference to compounds of the formula (XII). Compounds of thX formula (X) may also be prepared by reacting a compound of the formula (XVII): EMI17.1 with a compound of the formula (XIV) EMI17.2 wherein: D is a good leaving group, and R2, R5 and R4 are as defined in formula (I). 'Jii: reaction is suitably carried out in an inert organic solvent, such as hexamethylphosphoramide or N,N-dimethylformamide, at room temperature, in the presence of a base, such as sodium carbonate or sodium hydride, and a source of alkali metal ions, such as an alkali metal halide. Suitable alkali halides include sodium iodide and lithium iodide. Suitable examples of D include tosylate, bromide or iodide. Preferably D is a tosylate residue. Compounds of the formula (XI) are either known compounds o may be prepared by a process analogous to those used for preparing known compounds. The compound o:P. formula (X) may also be prepared be cyclising a compound of the formula (XIX) EMI18.1 wherein R2, R3, R4 and R5 are as defined in formula (I) and R6 is an organic group of up to 12 carbon atoms. Suitable groups R6 include C1,6 alkyl. The reaction is suitably carried out in an inert organic solvent, such as benzene or toluene at room temperature in the presence of a strong organic base such as an alkali meial alkoxide, for example potassium tert-butoride, or a t)o1ycyclic aza-substituted base, such as DBU (diazabicycloundecene). When R5 is not a hydrogen atom, the compound of formula (XIX) is conveniently prepared, and subsequently cyclised in situ, by the reaction of a compound of formula (XX): EMI18.2 with a compound R5NCO, conveniently in an inert organic solvent sch as toluene, under reflux. The compound of formula (XX) may be prepared by the N-nitrosylation and reduction of a compound of the formula (XXI) in conventional manner: EMI19.1 One suitable reaction scheme for the preparation of compounds of the formula (XXI) is shown below: EMI19.2 Process Variant b), Intermediates Compounds of to formula (XII) may be prepared by a process which comprises reacting a compound of the formula (XVI) as defined with a compound of the formula (XI) as defined. Compounds of the formula (XVI) may be prepared by reacting a compound of the formula(XVII): EMI20.1 with a strong base and a compound of formula (XV): EMI20.2 Compounds of the formula (XVII) are either known compounds ( .rc1 be prepared in analogous manner to known compounds. For example, one suitable reaction scheme for the preparation of these compounds is shown below: EMI20.3 Process Variant c) Intermediates Wnen R5 is not a hydrogen atom, the compound of formula (XIII) wherein Y is trans- CH=CH or -CH2CH2 may be prepared by reacting a compound of formula (XVIII): EMI21.1 with a compound of formula EMI21.2 and then if necessary reducing the resulting.Y is CH=CH compound to the corresponding Y is CH2CH2 compound. This reaction is suitably carried out in an inert organic solvent, such as benzene, at the reflux temperature, under an inert atmosphere. It should be mentioned that to prepare a compound of the formula (XII I) wherein R1 is other than hydrogen, it is generally preferred to prepare the corresponding compound of the formula (XIII) wherein RI is @@ogen and then to convert that compound to the desired 'mipound by conventional methods. For example a R1 is hydrogen compound may be converted to a R1 is methyl compound by treatment with acetyl chloride in methanol. The optional reduction can be carried out in conventional manner. When R5 is not a hydrogen atom, the compound of formula (XVIII) may be prepared by treating a compound of formula (XVII)as hereinbefore defined, that is, a compound of the formula: EMI21.3 with an oxidizing agent, such as N204 or t-butyl hypochlorite. This reaction is suitably carried out by suspending the chosen compound of the formula(XVII) in an inert organic solvent, such as dichloromethane, at OOC, and bubbling N204 through this suspension, or adding a known volume of N204 in dichloromethane slowly to the suspension. The preparation of the compounds of the formula(XVII) has been discussed hereinbefore. It is believed that compounds of formula (X), (XII), (XIII) and (XVI) are novel compounds, and these compounds are useful intermediates as hereinbefore described. As such, they form an important part of this invention. It will be realised by the skilled reader that although the reaction sequences leading to the active compounds of the invention hereinbefore described are particularly suitabie, a number of variations in the sequences are posible. It is believed these variations are best illustrated by use of the following flow diagram. (The reactions represented by arrows in the flow diagram are carried out as hereinbefore described, or in an analogous manner). EMI23.1 I-t will of course be realised -that the compounds of the formula (I) have an asymmetric centre, and thus are capable of exis-ting in enantiomeric forms. The invention extends -to each of these isomeric forms, and to mixtures thereof. The different isomeric forms may be resolved by the usual methods. Compounds within the formula (I) have useful pharmacological activity. For example compounds within the formula (I) have anti-gastric secretion activity e.g. anti-ulcer activity, cardiovascular activity e.g. anti-hypertensive activity or an-ti-arrhy-thnic activity,platelet aggregation inhibition activity, affect the respiratory tract e.g. broncho dilator activity, and have anti-fertility and smooth muscle activity. In general it may be said that compounds within the formula (I) have a range of pharmacological activities similar to those shown by the natural prosl;ag:landins, but that these activities tend -to be rathi' more selective. Compounds of the formula (I) wherein Q in the group R4 is an oxygen atom include compounds which in particular exhibit anti-gastric secretion activity, e.g. anti-ulcer activity, especially these compounds of formula (IV) The invention therefore also provides a pha@maceutical composition comprising a compound of the formula (I) and a pharmaceutically acceptable carrier. Clearly the formulation of the said pharmaceutical composition will depend on the nature of the activity shown by the chosen compound of the formula (I), and on lother factors such as preference in a particular area of therapy for a particular n.ode of administration. The compositione may be in the form of tablets, capsules, powders, granules, lozenges or liquid preparations, such as oral or sterile parenteral solutions or suspensions. Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipient; such as binding agents, fillers, tabletting lubricants, disintegrants and acceptable wetting agents and the like. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconsititution with water or other suitable vehicle before use. Such liquid preparations may contain conven-tional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), pre.servatives, and if desired conventional flavouring or colouring agents, and the ike. Por @@enteral administration, fluid unit dosage forms may prepared utilizing the compound of the formula (I) and a s-terile vehicle. The compound, depending on the vehicle and concen-tration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved for injection and filtcr sterilized before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents can be dissolved in the vehicle. Parenteral suspensions are prepared in substantially the same manner except that the, compound is suspended in the vehicle instead of being dissolved and s-terilization cannot be accomplished hy filtration. The compound can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or vetting agent is included in the composition to facilitate uniform distribution of the compound. When appropriate, the compositions of this invention may be presented as an aerosol for oral administratsion, or as as a mierofine powder for insufflation. As is common practice, the compositions will usually be accompanied by written or printed directions for use in the medical treatment concerned. It will of course be realised that the precise dosage used in the treatment of any of -the hereinbefore described disorders will depend on the actual compound of the formula (I) used, and also on other factors such as the of the disorder being treated. The invention also provides a methods o, treatment and/or prophylaxis of disorders in humae ' -'ings or demestie animals which comprises the administration to the sufferer of @@ @@ective amount of a compound of the formula (I). The following 3xamples 4, 5 and 8 illustrate the preparation of the invention. The following Examples 1 to 3, 6 and 7 illustrate the preparation of intermediates therefor. EXAMPLE I Preparation of 1-(p-Toluenesulphonyloxy)-3-methyl-4-phenoxybutan-3-ol. EMI28.1 To a slurry of zinc (33g, 0.5 mol) in benzene (50ml) containing a small crystal of iodine and under reflux with stirring, was added carefully 40ml of a mixture of phenoxypropan-2-one (44g, 0.29 mol), ethylbromoacetate (84g, 0.5 mol) and benzene (50ml) after reaction initiation the rest of the mixture was added at such a rate that reflux was maintained. After complete addition of the ketone mixture, the resultant reaction mixture was boiled at reflux for a further Ihr. The mixture was then cooled and poured into ice-cold 20% sulphuric acid (200ml) and ex traced with diethyl ether (4 x 200ml). The combined extracts were washed with water (2 x 250ml), saturated sodium hydrogen carbonate solution (2 x 250ml) and saturated sodium chloride solution until neutral. The extract was then dried (Na2S04), filtered and the filtrate concentrated in vacuo to leave an oil (65g). This residual oil was then distilled to afford ethyl -3-hydroxy-3-methyl-4- phenoxybutyrate (35.3g) b.p. 133-7 /0.05 mm. To a slurry of lithium aluminium hydride (5.64g, 0.148 mol) in diethyl ether (250ml) under nitrogen and cooled in an ice bath was added dropwise ethyl-3-hydroxy-3-methyl-4phenoxybutyrate (35.3g, 0.148 mol) in diethyl ether (100ml). After complete addition of the ester solution the resultant mixture was toiled at reflux for Ihr, then cooled in an ice bath. Excess lithium aluminium hydride was destroyed by successive dropwise addition of water (6ml), 10% sodium hydroxide solution (6ml) and water (18ml). The reaction mixture was filtered, the filter cake washed with diethyl ether (2 x 100ml), and the filtrate washed with saturated sodium chloride solution (1 x 250m1) and then dried (Na2SO4). This mixture was filtered and the filtrate concentrated in vacuo to give 3-methyl-4-phenoxybutane-l, 3-diol (26.5g) as an oil. N.m.r. (CDC13) # : 2.68-3.38 bm, 5H, C6H5-; 5.82-6.42m, 6H, CH2-OH, PhOCH2, OH x2 ; 8.02-8.38m, 2H, CH2; 8,73s, 3H, CH3. I.r (cm ) : 3500, OH; 1603, Ar. The 3-methyl-4-phenoxybutane-1,3-diol (26.5g, 0.135 mol) was dissolved in pyridine (150ml) and cooled to OOC with stirring p-Toluenesulphonyl chloride (27g, 0.142 mol) was added portionwise, and the resultant mixture was stirred for 45 minutes at 0 C. The reaction mixture was stored in ( refrigerator for 15hr and then poured into icedwater (200ml). The reaction mixture was extracted with diethyl ether (3 x 200ml) and the combined extracts washed with 10% hydrochloric acid (2 x 200ml), saturated sodium chloride solution (3 x 200ml) and then dried (Na2SO4). This mixture was filtered and the filtrate concentrated in vacuo at room temperature to leave a gum (38g). This gum was mixed with petrol (bp 60-80 C, 200ml), gradually cooled to -780C with stirring and the petrol decanted. Residual petrol was removed by evaporation in vacuo at room temperature to leave 1-(p-toluenesulphonyloxy)-3-methyl- 4-phenoxybutan-3-ol (38g) as a gum. Analysis: C, 62.01; H, 6.49; S, 9.53* C18H2205S requires: C, 61.71; H, 6.33; S,9.13%. N.m.r. (CDCl3) # : 2.23-3.32m, 9H, aromatic; 5.81t, 2H, CH2-OTs; 6.35s, 2H, PhOCH2; 7.23bs, 1H, -OH; 7.64s, 3H, EMI30.1 8.08t, 2H, CH2-CH2-OTs; 8.78s, 3H, CH I.r (cm 1) : 3600, -OH; 1603, aromatic; 1190, EMI30.2 EXAMPLE 2 Preparation of 4,4-dimethyl-8-methoxy-oct-1-en-3-one. EMI31.1 Sodium (53.2g, 2.32g. atom) was dissolved in methanol (500ml) and the resultant solution was added dropwise, with stirring, to 1,4-dibromobutane (500g, 2.32 mol) at 600. The resultant mixture was boiled at reflux for 6hr , cooled and filtered. The filtrate was poured into water (1200ml) and extracted with diethyl ether (4 x 600ml). The combined extracts were washed with water (2 x 300ml), dried (Na2SO4), filtered and concentrated in vacuo. The residue was fractionally distilled to afford 1-bromo-1X-methoxybutane 65.1g) bp. 52-6000, 15mm. Hg. N. . (CDCl3)#: 6.50-6.73m, 6.73's, 7H, BrCH2(CH2)2CH20CH3; 7,80-8.50m, 4H, BrCH2(CH2)2CH2OCH3. To ice-cold cyclohexylamine (198g, 2.0 mol) was added dropwise, with stirring, isobutyraldehyde (144g, 2.0 mol) and the mixture stirred at 0 for 3hr . To the reaction mixture was then added potassium carbonate (100g) and benzene (200ml) and the resultant mixture stirred overnight at room temperature. The mixture was then filtered and the benzene removed by distillation at atmospheric pressure. The residual oil was then distilled to afford,N-(2-methyl propylidene)cyclohexylamine (320g) bp 81-5 /2Omm.(ref bp 820/26mm). To a solution of lithium diethylamide prepared from lithium (2.98g, 0.43 mol) and diethylamine (32.0g, 0.43 mol), in hexamethylphosphoramide (92ml), benzene (84ml) and tetrahydrofuran (80ml) cooled to -780 under nitrogen, was added dropwise, with stirring N-(2-methylpropylidene) cyclohexylamine 68g, 0 39 mol) in tetrahydrofuran (80ml). The resultant mixture was allowed to warm to 0 during 0.5 h and was then re-cooled to -78 C and stirred for a further Ih at 7800. To this reaction mixture was added dropwise 1-bromo-4-methoxybutane (65.1g, 0,39 mol) in tetrahydrofuran (80ml) and the resultant solution was allowed to warm to room temperature and stirred at this temperature for 2 hr . Water (100ml) followed by 5N hydrochloric acid (20ml) were then added, and the mixture was boiled at reflux for 0.5hr. The mixture was then cooled and extracted with diethyl ether (4 x 200ml). The combined extracts were washed with 10% hydrochloric acid (2 x 200ml), water (2 x 200ml) and saturated sodium chloride solution until neutral, and then dried (Na2S04) and filtered, and the filtrate was concentrated in vacuo, to afford an oil (84g). This residual oil was distilled to afford 2,2-dimethyl-6-methoxyhexanal (36g) bp. 84-92/15mm. N.m.r. (CDCl3)#: 0.66, 1H, -CHO; 6.58-6.78m, 6,78s, 5H, CH2-OCH3; 8.4 - 8.78m, 6H, (CH2)3; 8.998, 6H, (CH3)2. To vinyl magnesium bromide prepared from magnesium (7.45g, 0.31 g atom), vinyl bromide (36.9g, 0.34 mol) in tetrahydrofuran (140ml), was added dropwise, with stirring, 2,2-dimethyl-6-methoxyhexanal (36g, 0.23 mol) in tetrahydrofuran (60ml), and the resultant mixture was stirred for 15hr at room temperature. The reaction mixture was poured into saturated ammonium chloride solution (200ml) and extracted with diethyl ether (4 x 200ml). The combined extracts were washed with saturated sodium chloride solution (2 x 250ml) and then dried (Na2S04) and concentrated in vacuo. The residual oil was distilled to afford I ,i -dimethyl-8- methoxy-oct-1-en-3-ol (31.7g) bp 120-127 , 14 mm Hg. N.m.r. (CC14) # 3.85-4.40m, 1H; 4.70-5.10m, 2H; 6.32d, 1H, CH-OH; 6.6-6.8m, 6.8s, 5H, CH2-0-CH3; 7.78 bs, 1H, -OH; 8.45-8.93 bm, 6H,2xCH3, 9.23d, 6H, (CH3)2. To a solution of 5,5-dimethyl-8-methoxy-oct-l-en-3-ol (31.7g, 0.17 mol) in acetone (200ml) at 0 C was added, dropwise during 2hr,a solution of chromium trioxide (17.5g, 0.17 mol) in water (52ml) containing concentrated sulphuric acid (15ml). The resultant solution was stirred for a further 0.5hr, then poured into water (100ml) and extracted with diethyl ether (4 x 200ml). The combined extracts were washed with saturated sodium chloride solution (4 x 200ml), dried (Na2S04) and IOmg hydroquinone added. The extracts were then filtered and the filtrate concentrated in vacuo. The residual oil was distilled to afford 5,5 dimethyl-8-methoxy-oct-l-en-3-one (15g), bp 112-124 , 14mm Hg. N.m.r. (CDCl3) # : 3.15q, J = 9, 17Hz; 3.70q, J = 3, 17Hz; 4.37q, J = 3, 9Hz; 6.5-6.69 bm, 6.69s, 5H; 8.17-8.84m, 8.84s, 12H. I.r (cm-1) : 1685, C=O; 1610, C=C. EXAMPLE 3 Preparation of 1-(6'-methoxycarbonyl-n-hexyl)-4-methyl-1, 2,4-triazolidine-3,5-dione. EMI34.1 Dinitrogen tetroxide was bubbled through a suspension of 4-methyl-1,2,4-triazolidine-3,5-dione2 (23..0g, 0.2 mol) in dichloromethane (250ml) cooled to 0o until a clear, homogeneous deep red solution was obtained. This solution was then dried (Na2S04) and filtered, and the filtrate was evaporated at room temperature in vacuo to give 4-methyl1,2,4-triazoline-3,5-dione (mp 1050, decomp.). To the triazoline (23.0g, 0.2 mol) dissolved in benzene (200ml) was added dropwise, with stirring, hept-6-enoic acid3 (23.0g, 0.18 mol) in benzene (100ml) and the resultant solution boiled under reflux in an atmosphere of nitrogen for Ihr. The reaction mixture was cooled and evaporated in vacuo. The residue was dissolved in a 10% solution of acetyl chloride in methanol (300ml) and boiled at reflux for 5hr and then left at room temperature overnight. The solution was evaporated and the residue (44g) was chromatographed on silica gel (Merck Kieselgel 60, 900g) using chloroform: methanol (0.5%) as eluant to afford 1-(6'-methoxycarbonyl n-hex-2-enyl)-4-methyl-1,2,4-triazolidine-3,5-dione (19.1g) m.p. 55-7 Analysis: C, 51.55; H, 6.56; N, 16.59% C11H17N304 requires C, 51.76; H, 6.71; N, 16.46%. N.m.r. (CDCl3) # : 0.8-1.2 bs, 1H, N-H; 4.37m, 2H, CH=CH; 5.9s,bd, 2H, N-CH2-; 6.40s, 3H, -CO2CH3; 7.00S, 3H, N-OH3; 7.5-8.8 bm, 6H, -(CH2)3. I.r (cm1) : 1760, EMI35.1 1670-1740, EMI35.2 CO2Me. Mass Spec. Meas. mass 255.1243; calc.mass 255.1219. The 1-(6'-methoxycarbonyl-n-hex-2-enyl)-4-methyl-1,2,4¯ triazolidine-3,5-dione (12.4g, 0.049 mol) was dissolved in dimethoxyethane (200ml),10% palladium on charcoal (3g) was added, and the resultant mixture allowed to take up hydrogen (ca. 1100ml). After the reaction was complete the resultant mixture was filtered through a Kieselguhr bed and the filtrate evaporated in vacuo to afford 1-(6' methOxyCarbonyl-n-hexyl)-4-methyl-1,2,4-triazolidine-3,5- dione (12.3g) mp 80-1 C. Analysis found: C, 51.12; H, 7.71; N, 16.38%. C11H19N304 requires C, 51.35; H, 7.44; N, 16.33%. N.m.r. (CDCl3)#: 6.398, 6,48m, 5H, CO2CH3; N-CH2; 6.978. 3H, N-CH3; 7.73m, 2H, CH2C02Me; 9.07 9.78 bm, 8H, (CH2)4. I.r (nujol, cm 1) : 1660-1780, EMI35.3 CO2Me. Mass Spec: Meas. mass 257.1377; calc. mass 257.1375. EXAMPLE 4 Preparation of 1-(6'-carboxyhexyl)-2-(3"-hydroxy-3"-methyl4"-phenoxybutyl)-4-methyl-1,2,4-triazolidine-3,5-dione EMI36.1 To a solution of 1-(6'-methoxycarbonyl-n-hexyl)-4-methyl- 1,2,4-triazolidine-3,5-dione (2.0g, 0.008 mol) in dimethylformamide (20ml) was added portionwise sodium hydride as an 80% dispersion in mineral oil (0.26g, 0.009 mol). After 0.5h and50 C under nitrogen, lithium iodide (2.3g, 0.018 mol) and a solution of 1-(p-toluenesulphenyloxy)-3-methyl-4- phenoxybutan-3-ol (2.73g, 0.008 mol) in dimethylformamide (20ml) were added and the resultant mixture stirred for 15hr at 50 C. T t' action mixture was then cooled and poured into water (200ml) and after acidification with 10% hydrochloric acid, the resultant mixture was extracted with ethyl acetate (3 x 200ml). The combined extracts were washed with saturated sodium chloride solution (3 x 250ml), dried (Na2SO4) ancl then filtered, and the filtrate was concentrated to give a gum (4.24g). This gum was chromatographed on silica gel (200g, Merck Kieselgel 60) using chloroform as eluant to afford 1-(6'-methoxycarbonyl-n-hexyl)-2-(3"- hydroxy-3"-methyl-4"-phenoxy-n-butyl)-4-methyl-1,2,4- triazolidine-3,5-dione (1.37g) as a gum. Analysis: C, 60.42; H, 7.59; N, 9.79%. C22H33N306 requires: C, 60.67; H, 7.64; N, 9.65% N.m.r. (CDCl3) # : 2.5-3.2m, 5H, C6H5; 6.0-6.77m, 6.15S, 6.31S, 9H, (N-CH2)2, CH2-OPh, OO2CH3; 6.94S, 3H, N-CH3; 7.26 bs, 1H, -OH; 7.70 bt, 2H, -CH2OO2Me; 7.90-9.0m, 8.648, 13H. I.r (cm 1) : 3500, OH; 1770, EMI37.1 1680-1760, EMI37.2 CO2Me; 1595, 1603, C=C. Mass Spec: Meas. Mass : 435.2338; Calc. Mass 435.2341. To a solution of 1-(6'-methoxycarbonyl-n-butyl)-2-(3"- hydroxy-3"-methyl-4"-phenoxy-n-butyl)-4-methyl-1,2,4triazolidine-3,5-dione (1.11g, 0.0026 mol) in methanol (40ml) was added a 10% aqueous solution of sodium carbonate (8ml) and the mixture boiled at reflux for 18hrs. The reaction mixture was cooled and evaporated in vacuo. The residue was dissolved in water (40ml) and extracted with ether (3 x 50ml). The aqueous phase was then acidified with 10% hydrochloric acid and re-extracted with ethyl aceLiL- (3 x 60ml). The combined extracts were washed with saturated sodium chloride solution (2 x 100ml), dried (Na2S04), filtered and the filtrate concentrated in vacuo to afford 1-(6'-carboxy-n-hexyl)-2-(3"-hydroxy-3"-methyl 4"-phenoxy-n-butyl)-4-methyl-1,2,4-triazolidine-3,5-dione (1.1g) as a gum. Analysis: C, 59.50; H, 7.75; N, 9.61%. C21H31N3O6 requires C, 59.84; H, 7.41; N, 9.97% N.m.r. (CDCl3) #: 2.5-3.2M, 5H, C6H5; 4.2 bs, 2H, CO2H, OH; 6.0-6.7 bm, 6.15s, 6H,"(N-CH2)2, -OCH2Ph; 6.94S, 3H, -N-CH3; 7.65 bt, 2H, OH2CO2H; 8.0-8.65 bm, 13H. I.r (cm-1); 2500-3450, CO2H, OH; 1760, EMI38.1 1540-1740, EMI38.2 CO2; 1603, C=C. Mass. Spec: Meas. mass. 421.2191; Calc. Mass, 421.2212. EXAMPLE 5 Preparation of 1-(6'-carboxyhexyl)-2-(4"-dimethyl3"-hydroxy-8"-methoxyoctyl)-4-methyl-1,2,4-triazolidine3,5-dione. EMI39.1 To a solution of 4-methyl-1,2,4-triazolidine-3,5-dione (5.0g, 0.0435 mol) in dimethylformamide (3Cml) was added portionwise sodium hydride as an 80% dispersion in mineral oil (1.44g, 0.048 mol). The resultant mixture was stirred at 50 C under N2 for 1hr and then a solution of 4,4-dimethyl8-methoxy-oct-1-en-3-one (8,0g, 0.0435 mol) in dimethylformamide (2Oml) was added dropwise. After 65hr at 50 C the rea@tion mixture was cooled and poured into ice-water (100ml), acidified with 10% hydrochloric acid and extracted with ethyl acetate (3 x 100ml). The combined extracts were washed with water (2 x 100ml), saturated sodium chloride solution (2 x 100ml) and then dried (Na2S04) and concentrated in vacuo to leave a gum (8.82g). This gum was dissolved in diethyl ether (100ml) and extracted with 2% aqueous sodium hydroxide solution (3 x 50ml). The combined extracts were washed with diethyl ether (2 x 100ml) and then acidified and re-extracted with ethyl acetate (3 x 100ml). The combined extracts were washed with saturated sodium chloride solution (3 x 200ml), dried (Na2SO4) and filtered, and the filtrate was concentrated in vacuo to give 2-(4', 4'-dimethyl-8'-methoxy-3'-oxo-n-octyl)-4-methyl-1,2,4-triazolidine-3,5-dione (5.45g) as a gum. N.m.r (CDCl3)# : 2.1 bs, 1H, N-H; 5.18t, 2H, N-OH2; 6.45-6.70m, 6.70S, 5HO OH2OCH3; 6.95S, 3H, N-CH3; 7.15t, CH2C-; 8.2-9.0 bm, 8.88S, 12H. T.r(cm ) : 3200, NH; 1760, C=O; 1700, EMI40.1 To a solution of 2-(4',4'-dimethyl-8-methoxy-3'-oxo-n- octyl)-4-methyl-1,2,4-triazolidine-3,5-dione (4,485g, 0.015 mol) in hexamethylphosphoramide (10ml), was added sodium carbonate (2.0g), sodium iodide (0.5g) and a solution of ethyl-7-bromo-heptanoate (3.91g, 0.016 mol) in hexamethylphosphoramide (10ml). Teh resultant mixture was stirred for 6 days at room temperature and then poured into water (100ml) and extracted with ethyl acetate (3 x 100ml). The combined extracts were washed with water (3 x 100ml) and saturated sodium chloride solution (2 x 100ml) and then dried (Na2S04) and filtered, and the filtrate was concentrated to leave an oil (7.75g). This oil was chromatographed on silica gel (Merck Kieselgel 60, 250g) using chloroform as eluant to afford an oil (2.93g). This oil was dissolved in ethanol (50ml) was sodium borohydride (0.268g, 0.067 mol) and added portionwise, and the resultant mixture was stirred overnight at room temperature. The reaction was then concentrated in vacuo, dissolved in water (50ml), acidified with 10% hydrochloric acid and extracted with ethyl acetate (3 x 50ml). The combined extracts were washed with saturated sodium chloride solution (2 x I0Oml), dried (Na2S04) and filtered, and the filtrate was concentrated in vacuo to afford a gum (2.92g). This gum was chromatographed on silica gel (Merck Kieselgel 60, 90g) to afford 1-(6'-ethoxycarbonyl-n-hexyl)-2-(4",4"- dime-thyl-3"-hydroxy-8"-methoxy-n-octyl)-4-mettyl-1,2,4-tria- zolidine-3,5-dione (1.87g) as a gum. N.m.r (CDCl3)#: 5.79q, 2H, CO2CH2CH3; 6.07-7.32 bm, 7.32S, 10H, (N-CH2)3, CH-OH, CH2-OCH3; 7.94S, 3H, N-CH3; 7.20 bs, 1H, -O-H; 7.22 bt, 2H, CH2C02Et; 8.07-8.87 bm, 19H; 9.15s, 6H. I.r (cm-1): 3450, -OH; 1770, EMI41.1 1660-1740, EMI41.2 CO2Et. to a solution of 1-(6'-ethoxycarbonyl-n-hexyl)-2-(4",4" dimethyl-3"-hydroxy-8"-methoxy-n-octyl)-4-methyl-1,2,4- triazolidine-3,5-dione (1.87g) in ethanol (20ml) was added a 10% aqueous solution of sodium carbonate (15ml) and the mixture was boiled at reflux for 18hr . The reaction mixture was cooled and evaporated in vacuo. The residue was dissolved in water (40ml) and extracted with diethyl ether (3 x 50ml). The aqueous phase was then acidified with 10% hydrochloric acid and re-extracted with ethyl acetate (3 x 60ml). The combined extracts were washed with saturated sodium chloride solution (2 x 100ml), dried (Na2S04) and filtered, and the filtrate was concentrated in vacuo to afford 1-(6'-carboxy-n-hexyl)-2-(4",4"-dimethyl-3" hyclrOxy-8"-methoxy-n-octyl)-4-methyl-1,2,4-triazolidine-3, 5-dione (1.32g) as a gum. N.m.r (CDCl3)#: 3.95 bs, 2H, -OH; 6.05-6.75 bm, 6.65S, 10H, CH-OH, (N-CH2)2, CH2-O-CH3; 6.918, 3H, -N-CH3; 7.65 bt, 2H, CH2CO2H; 8.0 8.9 bm, 16H, 9.13 bs, 6H. I.r (cm 1) : 2500-3450, -OH; 1760, EMI41.3 1640-1740, EMI41.4 -CO2. EXAMPLE 6 Preparation of 4,4-dimethylhepta-1,6-dien-3-one EMI42.1 2,2-Dimethylpent-4-enal was prepared as described by K. C. Braunsch, J. Amer. Chem. Soc., 1959, 81, 3379. To vinyl magnesium bromide, prepared from nagnesium (13.4g, 0.56 mol) and vinyl bromide (66.4g, 0.62 mol) in tetrahydrofuran (100ml), was added dropwise a solution of 2,2-dimethylpent-4-en-l-al (46.3g, 0.413 mol) in tetrahydrofuran (100ml). The resultant mixture was stirred overnight at room temperature, poured into saturated ammonium chloride solution (200ml) and extracted with diethyl ether (3 x 200ml). The combined extracts were washed with saturated sodium chloride solution (2 x 200ml), dried (Na2S04), filtered an(1 ( ncentrated in vacuo to give an oil (52g). This oil was distilled to afford 4,4-dimethylhepta-1,6-diene-3-ol (45.4g) b p. 64-68 /14 nm Hg. To a suspension of pyridinium chlorochromate (104.5g, 0.486 mol) in dichloromethane (500ml) stirred at room temperature was added rapidily a solution of 4,4-dimethylhepta-1,6-dien-3-ol (45.4g, 0.324 mol) in dichloromethane (70ml) and cooled in an ice-bath. The reaction mixture was stirred for 2hr at room temperature after subsidence of the initial exothermic reaction, diethyl ether (250ml) was added, and the mixture was filtered. The filtrate was concentrated in vacuo, hydroquinone (100mg) added and the residual oil was distilled to afford 4,4dimethylhepta-1,6-dien-3-one (24.7g) bp. 58-62 /15 mm Hg. EXAMPLE 7. Preparation of 2-(4',4'-dimethyl-3'-oxo-hepta-6'-enyl)-4methyl-1,2,4-triazolidine-3,5-dione. EMI43.1 To a solution of 4-methyl-1,2,4-triazolidine-3,5-dione (6.9g, 0.06 mol) in dimethylformamide (70ml) stirred at room temperature, under nitrogen, was added portionwise sodium hydride (1.98g, 0.066 mol) as an 86% dispersion in mineral oil. The resultant mixture was stirred at 50 C for 6hr and then a solution of 4,4-dimethylhepta-1,6- dien-3-one (8.28g, 0.06 mol) in dimethylformamide (10ml) was added dropwise. After 3 days at 50 C, the reaction mixture was cooled and poured into water (200ml), and the resultant mixture was acidified with 10% hydrochloric acid, and extracted with ethyl acetate (3 x 100ml). The combined extraets were washed with water (3 x 100ml) and saturated $sodium chloride solution (2 x 100ml) and then dried (Na2S04) and filtered, and the filtrate was concentrated in vacuo to give an oil. This oil was dissolved in diethyl ether (100ml) and extracted with 2% aqueous sodium hydroxide solution (3 x 50ml). The combined extracts were extracted with diethyl ether (2 x 100ml), acidified and re-extracted with ethyl acetate (3 x 100ml). The combined ethyl acetate extracts were washed with saturated sodium chloride solution (3 x 100ml) and then dried (Na2S04) and filtered and the filtrate evaporated in vacuo to give 2-(4',4'-dimethyl-3'-oxo-hept-6'- enyl)-4-methyl-1,2,4-triazolidine-3,5-dione as a gum (4.403g). N.m.r (CDCl3)# 1.80 bs, 1H, -N-H; 3.96-4.62m, 1H, 4.75 5.17m, 2H; 6.18t, 2H, -N-CH2; 6.92S, 3H, O -N-CH3; 7.12t, 2H, CH2-C; 7.70d, CH2- ; 8.85S, 6H. I.r (cm-1) : 2700-3300, N-H; 1760, EMI44.1 1660-1720, 0 N-C. EXAMPLE 8 Preparation of 1-(6'-ethoxy -n-hexyl)-2-(4",4"-dimethyl 3"-hydroxy-hept-6"-enyl)-4-methyl-1,2,4-triazolidine-3,5- dione. EMI45.1 To a solution of 2-(4',4'-dimethyl-3'-oxo-hept-6'-enyl)-4 methyl-1,2,4-triazolidine-3,5-dione (3.58g, 0.014 mol) in hexamethylphosphoramide (10ml) was added sodium carbonate (2.0g), sodium iodide (0.5g) and a solution of ethyl7-bromoheptanoate (4.12g, 0.017 mol) in hexamethylphosphor- amide (10ml). The resultant solution was stirred at room temperature for 10 days and then poured into water (100ml) and extracted with ethyl acetate (3 x 100ml). The combined extracts were washed with water (3 x 100ml) and saturated sedium chloride solution (2 x 100ml) and then dried (Na2S04) and filtered and the filtrate was evaporated in vacuo to leave a gum (5.98g). This gum was dissolved in ethanol (75ml), and sodium borohydride (0.611g, 0.016 mol) was added portionwise. The resultant mixture was stirred overnight at room temperature and then concentrated in vacuo. The residue was dissolved in water (100ml), acidified with 10% hydrochloric acid and extracted with ethyl acetate (3 x 100ml). The combined extracts were washed with saturated sodium chloride solution (2 x 100ml) and then dried (Na2S04) and filtered and the filtrate concentrated in vacuo to leave a gum (5.89g). This gum was chromatographed on silica gel (Kieselgel 60, 180g) using chloroform as eluant to afford 1-(6'-ethoxycarbonyl-n-hexyl)-2-(4",4"-dimethyl-3"- hydroxy-hept-6t'-enyl)-4-methyl-1,2,4-triazolidine-3,5-dione (2.0g) as a gum. N.m.r (CDCl3) # : 3.96-4.52m, 1H, 4.88-5.1m, 2H; 5.78q, 2H; 6.28m, 5H; 6.82S, 3H, 7.34 bs, 1H; 7.45 8.2 bm, 8.65t, 17H, 9.02d, 6H. I.r (cm-1) : 3450, -OH; 1760, EMI46.1 1660-1740, EMI46.2 CO2Et. PHARMACOLOGICAL DATA Broncho-dilation Activity The compound of Example 4 was examined for its ability to inhibit 5-hydroxy-tryptamine or histamine induced bron choconstriction in the anaeshetised artifically respired guinea-p;g (Konzett-Rossler preparation). The ED50 value (g/kg, i.v.) for inhibition of bronchoconstriction for the compound of Example 4 was 39. Anti-ulcer activity Method An-ti-ulcer activity of the compound of Example 4 was assessed by the inhibition of indomethacin induced gastric damage in the rat according to the method of Eleghe (1974) Israeli J. Med. Sci. 10. 1451. Rats were starved overnight, given 15mg/kg indomethacin subcutaneously and sacrificed 4 hours later. Stomachs were reflated with n. saline, cut along the greater c:lrv;lture pinned out and scored for gastric damage. Groups of 7 rats were used for each treatment and the test compound or vehicle was administered 30 minutes prior to giving the indomethacin. Varying doses of test compound were administered orally and control groups receiving vehicle only were set up simultaneously. Mean values for each treatment were obtained and the Mann Witney test applied for significance of difference between the values obtained with the treatments. The results are shown in Table A. Table A EMI48.1 <tb> <SEP> Dose <SEP> % <SEP> <SEP> Inhibition <SEP> of <tb> mg/kg <SEP> p.o. <SEP> damage <tb> <SEP> 100 <SEP> 92 <tb> <SEP> 50 <SEP> 50 <tb> <SEP> 25 <SEP> # <SEP> <SEP> 56 <tb> Anti-gastric secretory activity The compounds were examined for their ability to inhibit pentagastrin-stimulated gastric acid secretion in the anaesthetised, perfused rat stomach preparation (Ghosh and Schild preparation, Parsons modification). The compounds were administered intra-venously. The results are shown in Table B Table B EMI48.2 <tb> <SEP> . <SEP> % <SEP> Inhibition <SEP> of <SEP> <tb> mg/kg <SEP> i.v <SEP> secretion <tb> <SEP> 0.4 <SEP> 60 <tb> <SEP> 1.6 <SEP> 98 <tb> Toxicity No toxic effects were observed in these tests.

Claims: CLAIMS 1. A compound of the formula (I): EMI49.1 characterised in that: n is 1 to 5; Y is -CH2-CH2, -CH=CH- or -C--C- R1 is hydrogen or C02R1 represents an ester group in which the R1 moiety contains from 1-12 carbon atoms; is hydrogen, C1-4 alkyl or phenyl; R3 is hydroxy or protected hydroxy; R4 is is a group of the formula: L - Q - R10 in which: Q is an oxygen atom, a -CH=CH- group or a -CC- group and L is a C1 9 alkylene group; and R10 is C1 4 alkyl, phenyl or nphthyl, any of which phenyl or naphthyl moieties may be @bstituted by one or more halogen, trifluoromethyl, C1-6 alkoxy, phenyl C1-6 alkoxy or nitro groups, or, if Q is a -CHL-CH- group, then R10 may be a hydrogen atom; and R5 is hydrogen or C1-6 alkyl; and salts thereof. 2. A compound according to claim 1 of formula (II): EMI49.2 characterised in that: Y, R1 and R5 are as defined in claim 1: n is 2, 3 or RÚ 1 is hydrogen, methyl, ethyl or phenyl; and R1 is a group of 2 the formula: 1 10 in which L is a C1 9 alkylene group; and RÚ10 is 1-4 alkyl; and salts thereof. 3. A compound according to claim 1 of the formula (III): EMI50.1 characterised in that: R1 is as defined in claim 1 RÚ2 is hydrogen, methyl or ethyl; and Rê4 is a group of the formula: LÚ - O - Rê10 in which LÚ is a C4-8 alkylene group, and Rê10 is C1-2 alkyl; and salts thereof. 4. 1-(6'-carboxyhexyl)-2-(4",4"-dimethyl-3"-hydroxy-8"-methoxyoctyl) 4-methyl-1,2,4-triazolidine-3,5-dione. g. A compound according to claim 1 of formula (IV): EMI50.2 characterised in that: Y, R1 and R5 are as deined in claim 1; n is 2, 3 or 4; RÚ2 is hydrogen, methyl ethyl or phenyl; and R 4 is a group of formula (V): EMI51.1 wherein: L is as defined in claim 2 and V, W and Z are each hydrogen or fluorine, chlorine or bromine atoms, or CF3, methyl, ethyl, n- or iso-propyl, methoxy, ethoxy, n- or :iso-propoxy or nitro groups; and salts thereof. 6. 1-(6'-carboxyhexyl)-2-(3"-hydroxy-3"-methyl-4"-phenoxybutyl) 4-methyl-1,2,4-triazolidine-3,5-dione. 7. A compound according to claim 1 of formula (VI): EMI51.2 baracterised in that: Y, nÚ, R1, RÚ2 and R5 are as defined in claim 1; and R44 is a group of the formula - L - QÚ - R 10 in which L is as defined in formula (I); Q is a -CH=CH- group or a -CC- group; R310 is C1-4alkyl, or if Q is a -CE=CH- group, then R310 may be a hydrogen atom; and salts thereof. 8. A compound according to claim 1 of formula (VII): EMI51.3 characterised in that: R1 is as defiled in claim 1; RÚ2 is hydrogen, methyl or ethyl; R54 is a group of the formula L - QÚ - R410 in which L is a C1-4 alkylene group Q1is a group -CH=CH- or a group -CC-; is is C. alkyl or may be hydrogen if Q1 is a -CH=CH- group; 10 and salts thereof. 9. 1-(6'-carboxyhexyl)-2-(4",4"-dimethyl-3"-hydroxyhept-6"-enyl) #-methyl-1,2,4-triazolidine-3,5-dione. 10. A compound according to claim 1 of formula (VIII): EMI52.1 characterised in that: 1, R1 and R5 are as defined in claim 1; n is 2, 3 or RÚ is hydrogen, methyl, ethyl or phenyl; R64 is a group of formula (IX): EMI52.2 wherein: L and Q are as defined in formula (VI); and V, W and Z are each hydrogen, chlorine or bromine atoms, or CF3, methyl, ethyl, n- or iso- propoxy or nitro groups, and salts thereof. 11. A pharmaceutical composition characterised in that it comprises a compound of the formula (I) as defined in claim 1 and a pharmaceutically acceptable carrier. 12. A process for the preparation of a compound of the formula (I) as defined in claim 1, characterised by either a) reacting a compound of formula (X): EMI53.1 wherein: R2, R3, R4 and R5 are as defined in claim 1, with a compound of formula (XI): T-CH2-Y-(CH2)nC02R1 (XI) wherein T is a good leaving group, and Y, n and R1 are as defined in claim 1. b) reacting a compound of the formula (XII): EMI53.2 wherein: Y, n, R1, R4 and R5 are as defined in claim 1; with a reducing agent to give a corresponding compound of the formula (I) wherein R2 is hydrogen and R3 is hydroxy, or with a C14 alkyl or phenyl, Grignard reagent or C14 alkyl or phenyl metallic complex to give a corresponding compound of the formula (I) wherein R2 is C1-4 alkyl or phenyl, and R is hydroxy; and then optionally protecting,the R3 hydroxy moiety, or c) when R5 in the compound of formula (I) is not a hydrogen atom, reacting a compound of the formula (XIII) EMI54.1 wherein: Y, n, R1 and R5 are as defined inclaim 1, with a compound of formula (XIV): EMI54.2 wherein the variables are as defined in claim 1.

Inventors: ADAMS, DAVID REGINALD, BARNES, ALLEN FRANK
Applicants: BEECHAM GROUP PLC
Application year: 1979
Main classification: C07D249
IPC codes: A61P9, C07C43, C07C47, C07C49, A61P1, A61K31, C07D249, C07C45, C07C41, C07C29, C07C309, A61P43, A61P7