6-AMINO PYRIDINE CARBOXYLIC ACID DERIVATIVES, A PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

Abstract: This invention provides a series of novel nicotinic acid derivatives, a method for their preparation and their use as hypolipidaemic agents. The compounds of the invention have formula (I):- wherein R₁CH₂- is a C₉ to C₂₅ alkyl or alkenyl group which is optionally branched or unbranched or includes a carbocyclic ring and R₂ is -CO₂H or -CH₂CO₂H or a pharmaceutically acceptable salt thereof or a pharmaceutically acceptable group which is convertable in the human body into a -CO₂H or -CH₂CO₂H group.

Description: 6-Amino nicotinic acid I)rivatives This invention relates to a series of novel nicotinic acid derivatives to a method for their preparation and to their use as hypolipidaemic agents. Accordingly the present iiivention provides a compound of formula (I): EMI1.1 wherein R1CH,- is a C9 to C25alkyl or alkenyl group which is optionally branched or unbranched or includes a carbocyclic ring and R3 2 is -CO2H or -CH2CO2H or a pharniaceutically acceptable salt thereof or a pharmaceutically acceptable group which is convert able in the human body into a-CO2H or -CH2CO2H group. Most suitably R1C142- does not contain more than 20 carbon atoms. Examples of suitable unbranched alkykl groups which R1CH9- may represent include 1-dodecyl, 1-tetradecyl, l-hexadecyl, l-octadecyl and l-eicosyl. Examples of branched alkyl groups include 3-dodecyl, 3-tetradecyl, 3-llexlctecyl, -oct a.lecyl alld 3-eicosyl. Ccjrb()cyclic riiigs \tliic-ii Cm 13 may include include nay Itmono- Li- or polycyclic. Examples of oi table mono-cyclic systelils includes cyclodecyl and cyclododecyl. Examples of suitable bi-cyc Lic systems include bicyclo [2.2.1] heptyl, bicyclo [2.2.1] hel)tenyl, bicyclu [2.2.2] octyl and bicycle [4.4.0] decyl. An example of a suitable polycycloalkyl group is adamantyl. Preferably R1 is an unbranched C13 to C alkyl group. Thus a preferred class of compounds within the scope of this invention has the general formula (I) wherein R is an unbranched C13 to C17 alkyl group and 2 is as previously defined with reference to formula (1). An example of a compound of this preferred class is 6-(n-hexadecylamino)-nicotinic acid. Examples of pharmaceutically acceptable salts of the -CO2H and -CH2CO2H include alkali metal and alkaline earth metal salts, particularly sodium, potassium, calcium and magnesium salts. Compounds of general formula (I) also form pharmaceutically acceptable acid addition salts with appropriate acids. Examples of suitable acids include inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulphuric, phosphoric and nitric acid and organic acids such as lower alkanoic acids (e.g. acetic and propionic), glycollic pyruvic, malonic, succinic, malic, tartaric and citric acids. The term pharmaceutically acceptable groups which are convertable in the human body into a -CO2H or -CH2CO2H group means a group which is hydrolysed or is metabolised in some other way to a -CO2H or -CH2CO2H group, and is such that the compound of formula (I) is pharmaceutically acceptable. Groups which are convertable by hydrolysis to the -CO2H or -CH2CO2H group include pharmaceutically acceptable esters. Examples of such esters include the C1 to C6 alkyl esters, in particular the methyl, ethyl and n-propyl esters. Other suitable esters include for example alkoxyalkyl esters such as methoxymethyl esters; acyloxyalkyl esters such as acetoxymethyl, pivaloyloxymethyl, a-acetoxyethyl, cL- acetoxybenzyl and a-pivaloyloxvethvl esters; alkoxycarbonyloxyalkyl esters, such as ethoxycarbonyloxyethyl; and lactone, thiolactone and dithiolactone esters, i.e. ester groups of formula (II): EMI3.1 wherein X' and Y' are oxygen or sulphur and Z' is an ethylene group or a 1,2-phenylene group optionally substituted by C1 - C6 alkoxy, halogen or nitro. Preferred ester groups are the phthalidyl and 3,4-dimethoxy phthalidyl esters. Typical of groups which are metabolisable to -CO2H and -CH2C02H groups are terminal methyl ketones where R2 in formula (I) represents: EMI3.2 in which n is an integer up to 5, terminal carbinols where R2 in formula (I) represents: - (CH2)nCH2OH in which m is zero or an integer up to 5 and terminal carboxylic acids where R2 in formula (I) represents: - (CH2)p.C02H in which p is an integer from 2 to 6, as well as their pharmaceutically acceptable salts and esters. Suitable pharmaceutically acceptable salts and esters of such terminal carboxylic acid containing derivatives are the same as discussed above with reference to salts and esters of the -COH and -CHCOH groups. Where n, m or p are odd the group is metabolised to -Cl42CO2H. Where n is zero and where m, n and p are even the group is metabolised to -CO2H. When R2 represent a terminal methyl ketone, m is preferably 1. An example of such a compound is 3-( 2-oxo-propyl )-6-( n-hexadecylamino)-pyridine. When R2 represents a terminal carbinol,n is preferably 1. An example of such a compound is 3 hydroxymethyl-6- (n-hexadecylamino ) -pyridine. Where R2 represents a terminal carboxylic acid, p is preferably 2. An example of such a compound is 3-(6 '-n-hexadeylaminopyrid-3'-yl) propanoic acid. Tn order to determine whether a group R2 is a group which is convertable in the human body to -CO2H or -CII2CO2H (as required by the above description), the urine of a patient to whom the compound of formula (I) has been administered is tested to detect the presence of a compound carrying -CO2H or -CH2C02H at that position, and which is absent in the urine of the same patient before such administration. In some cases it may be necessary to administer the compound carrying a radio-active label on the &alpha;-carbon in the group R2, to see if a compound is excreted bearing a labelled carboxylic acid group. Compounds of formula (I) may be prepared by reacting a halopyridine derivative with a suitable amine. Accordingly,the invention further provides a process for preparing a compound of formula (I) which process comprises reacting a compound of formula (III): EMI4.1 with an amine of formula R CII2NI-I, wherein X is a haloge atom and R1 and R2 are defined with reference to formula (I) above, and thereafter tviere desired converting a compound of formula (I) so produced as a free carboxylic acid into a pharmaceutically acceptable salt or ester, converting a compound of formula (I) so produced as a salt into a pharmaceutically acceptable ester, or converting a compound of formula (I) so produced as an ester into a salt or free carboxylic acid. Examples of suitable bases include alkali metal and alkaline earth metal carbonates and hexamethylphosphoramide. Preferably the base is a mixture of potassium carbonate and hexamethylphosphoramide. The reaction is preferably carried out at elevated temperatures i.e. greater than 100 C, 120140 C being the most convenient temperature range. The reaction may be carried out in the presence or absence of solvent. Generally where hexamethyl phosphoramide is the base the reaction is carried out in the absence of solvent. The time for which the reaction is allowed to proceed depends upon the nature of the starting materials and the base and the temperature at which it is carried out. Suitable reaction times may be determined by following the course of reaction with any standard technique such as thin layer chromatography. Generally, under the preferred conditions referred to above, the reaction is complete within 3 to 5 hours. The compounds of this invention may also be prepared by alkylating an aminopyridine with an alkyl halide. Accordingly the present invention provides a process for preparing a compound of -formula (I) above which process comprises reacting a compound of formula (IV):- EMI6.1 with an alkyl halide R1X, wherein R1 and R2 are as declined with reference to formula (I) and X is halogen in the presence of a base, and thereafter where desired converting a compound of formula (I) so produced as a free carboxylic acid into a pharmaceutically acceptable salt or ester, or converting the compound of formula (I) so produced as a salt into a free acid or ester, or converting a compound of formula (I) so produced as an ester into a free acid or salt. This process is carried out in a manner analogous to that described above with reference to compounds of formula (III). Compounds of formula-(I) (I) above may also be prepared by reducing a corresponding enamine (V): EMI6.2 wherein R1 and R2 are defined with reference to formula (I) with a reducing agent and thereafter where desired converting a compound of formula (I) so produced as a free carboxylic acid into a pharmaceutically acceptable salt or ester,or converting the compound of formula (I) so produced as a salt into a free acid or ester, or converting a compound of formula (I) so produced as an ester into a free acid or salt. A suitable reagent to effect this reduction is a metal hydrirle such as sodium borohydride in an alcoholic solvent, particularly ethyl alcohol. Alternatively, catalytic hydrogenation may be employed, for example using a platinum oxide catalyst. The intermediates of formula (V) may be prepared by reaction of an amine of formula (VI) with an aldehyde of formula (VII): EMI7.1 It may be convenient to generate the intermediate (V) in situ, by reacting an amine of formula (VI) with an aldehyde of formula (VII) in the presence of a reducing agent. In such a case, a suitable reducing agent is hydrogen on a platinum oxide catalyst in a suitable solvent such as a mixture of methanol and dioxan. Compound of formula (I) may also be prepared by reducing a compound of formula (VIII): EMI7.2 wherein R1 and R2 are as defined with reference to formula (I) and thereafter where desired converting a compound of formula (I) so produced as a free carboxylic acid into a pharmaceutically acceptable salt or ester or converting a compound of formula (I) so produced as a salt into a free acid or ester, or converting a compound of formula (I) so produced as an ester inl() a free acid or salt. A suitable reagent for reducing compound (VIII) is borane in dimethyl sulphide. Intermediates (VIII) may be prepared by acylation of a compound (VI) as defined above in a conventional manner. Compounds (I) produced as free acids or salts in the above processes may be converted to esters by conventional methods. Similarly esters and salts may be converted into free acids and salts may be converted to esters by conventional methods. In order to use the compounds of formula (I) as hypolipidaemic agents they may be formulated as pharmaceutical compositions in a variety oi dosage forms. Accordingly, in a further aspect, the invention provides a pharmaceutical composition comprising a compound of formula (I) as defined above or a pharmaceutically acceptable salt or ester thereof -together with a pharmaceutically acceptable carrier. The compositions may be formulated for systemic administration by any route, although oral administration is preferred. The conipositions may be in the form of tablets, capsules, powders, granules, lozenges or liquid preparations, such as oral or sterile parenteral solutions or suspensions. Tablets and capsules for oral administration may be in unit dose presentation iorm, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacantli, or polyvinyl-pyrollidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known iri normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example, sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia, non-aqueous vehicles (which may include edible oils) for example almond oil, Iractionated coconut oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl -hydroxybenzoate or sorbic acid; and if desired conventional flavouring or colouring agents. Suppositories will contaiii conventional suppository bases, e.g. cocoa butter or other glycerides. For parenteral administration, fluid unit dosage forms are prepared utilizing the compounds and a sterile vehicle, water being preferred. Tulle coiripound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved in water for injection and filter-sterilized before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as local anaesthetic, preservative and buffering agents can be dissolved in the vehicle. To enhance the stahility, the composition can be frozen after filling into the vial and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The compound can be sterilized by exposure tl) ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound. The composition may contain from 0.1% to 99 by weight, preferably from 10-60zEo by weight, of the active material, depending on the method of administration. Where the compositions comprise dosage units, each unit will preferably contain from 250 mg - 3g, of the active ingredient. The dosage as employed for adult human treatment will preferably range from 1 to lOg, per day, for instance 39, per day, depending on the route and frequency of administration. The following examples illustrate the invention: Example J Preparati)n of methyl-6- ( n-hexadecylamino) -pyricline-3- carboxylate ate Methyl-6-chloropyridine-3-carboxylate (3.449; 0.02 m) was added to a mixture of n-hexadecylamine (6;059; 0.025 m) and anhydrous potassium carbonate (69) in hexamethylphosphoramide (30 ml). The mixture was heated at 130 C for 90 minutes with vigorous stirring, cooled to room temperature,and water (1JO X was added. The solution was extracted with dichloromethane (2 x 100 ml) and the organic extracts were dried (WlgSO) and evaporated to dryness. The residual oil was dissolved in diethyl ether (100 ml),washed with water (2 x 100 nil), and the organic phase was dried (MgSO4) and evaporated to give 6.99 of solid product. Crystallisation of the product from ether yielded methyl-6- ( n-hexa decylamino) -pyridine-3-carboxylate (3.99; 52%) M.P. 88-900C. Analysis Req. Fc)un(l C 73.40 73.09 H 10.64 10.82 N 7.45 7.32 Example 2 Preparation of 6-(n-hexadecylaminci)-pyridine-3-carboxylic acid I jvdrochloride Methyl-6- (n-hexadecy lamino) -pyridine-3-carboxylate (7.52 g ; 0.02 ìTI) in methanol (20 ml) was treated with potassium hydroxide (5.6 g; 0.1 m) in water (20 ml) and the mixture was boiled under reflux with stirring for 90 minutes. The product was cooled, the solid filtered, i:ashed with water (50 ml) and dried to give the potassium salt. This salt was suspended in 10, hydrochloric acid solution (100 ml) and boiled under reflux with stirring for 1 hour. The solution was cooled, diluted with water (200 ml), filtered,and the solid product dried under vacuum to give 6-(n-hexadecyclamino)-pyridine-3-carboxylic acid hydrochloride (5.lg ; 64%) M.P. - 150 C 1500C Decomp. Analysis Req. Found C 66.25 67.00 H 9.78 9.92 N 7.02 6.94

Claims: CLAIMS 1. A compound of formula (I): EMI13.1 wherein R1CH2- represents a Cg to C25 alkyl or alkenyl group which is optionally branched or unbranched or includes a carbocyclic ring and R2 represents a -CO2H or -CH2CO2H group or a pharmaceutically acceptable salt thereof or a pharmaceutically acceptable group which is convertable in the human body into a -CO2H or -C02H group. 2. A compound as claimed in claim 1 wherein the group P1 CH2- contains not more than 20 carbon atoms. 3. A compound as claimed in claim 1 wherein R represents an unbranched C13 to C17 alkyl group. 4. 6- (n-Hexadecylamino)-pyridine-3-carboxylic acid, or a C16 alkyl ester thereof, or a pharmaceutically acceptable acid addition salt of the acid or ester thereof. 5. A process for the preparation of a compound as claimed in claim 1, which process comprises: a) reacting a compound of formula (III): EMI13.2 with an amine of formula R1CH2NH2 wherein X is s a halogen atom arid R1 and R2 are as deiined in c ai ri 1: or h) reacting a compound of formula (IV): EMI14.1 jith an alkyl halide of formula R1X, wherein R1 and R2 are as defined in claim 1 and X is a halogen, in the presence of a base; or c) reducing an enamine of formula (V) or an aside of formula (VIII): EMI14.2 wherein R1 and R2 are as defined in clairn 1, with a reducing agent; and after processes a.), b) or c), then optionally converting a compound of formula (I) so produced as a free carboxylic acid into a pharmaceutically acceptable salt or ester, converting a compound of formula (I) so produced as a salt into a free acid or a pharmaceutically acceptable ester, or converting a compound of formula (I) so produced as an ester into a salt or free carboxylic acid. 6. A pharmaceutical composition which comprises a compound as claimed in claim 1 together with a pharmaceutically acceptable carrier.

Inventors: HINDLEY, RICHARD MARK
Applicants: BEECHAM GROUP PLC
Application year: 1979
Main classification: C07D213
IPC codes: A61P3, A61K31, C07D213