Freshwater Fishing. License: A Florida freshwater fishing license is required to fish in freshwater or to possess fresh water species. Bait: Live or dead fish (including minnows and shiners) or amphibians, and non-preserved fish eggs or roe, are prohibited. Digging for bait inside the park is not permitted. Closed to Fishing: No fishing is allowed at the Ernest F. Coe (Main) Visitor Center lakes, Royal Palm Visitor Center area and trails, Chekika Lake, along the first 3 miles of the Main Park Road, including Taylor Slough, or along the Shark Valley Tram Road.