Background: There is growing concern regarding the use of pesticides in agriculture. Therefore, many of the current commercial applications of modern biotechnology in agriculture are focused on reducing the dependence of farmers on these chemicals. For example, Bacillus thuringiensis (Bt) is a soil bacterium that produces a protein that can act as an insecticide, known as the Bt toxin . But it is a naturally occurring protein, not a foreign chemical. Could this protein be used in crops instead of pesticides? Traditionally, an insecticidal spray has been produced from these bacteria. As a spray, the Bt toxin is in an inactive state and requires digestion by an insect to become active and have any effect. Crop plants have now been engineered to contain and express the genes for the Bt toxin, which they produce in its active form. When an insect ingests the transgenic crop, it stops feeding and soon thereafter dies as a result of the Bt toxin binding to its gut wall. Bt corn is now commercially available in a number of countries to control corn borer (an insect like a moth or butterfly), which is otherwise controlled by insecticidal spraying.

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer used a bacterium that produces a protein that can act as an insecticide?
Answer: Don

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer didn't use a bacterium that produces a protein that can act as an insecticide?
Answer: Nate

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer didn't use a bacterium that produces Bt toxin?
Answer: Nate

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer used a bacterium that produces Bt toxin?
Answer: Don

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer used a naturally occurring protein, not a foreign chemical?
Answer: Don

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer didn't use a naturally occurring protein, not a foreign chemical?
Answer: Nate

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer didn't use a an insecticidal spray produced from bacterial?
Answer: Nate

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer used a an insecticidal spray produced from bacterial?
Answer: Don

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer used a an insecticidal spray  that is in an inactive state and requires digestion by an insect to become active?
Answer: Don

Situation: Two farmers tried different methods of growing their crops. Nate used pesticides, while his buddy Don used bacterium that produces a toxin instead. Don was happy with the results.

Question: Which farmer didn't use a an insecticidal spray  that is in an inactive state and requires digestion by an insect to become active?
Answer: Nate