Background: An anaerobic organism is any organism that does not need oxygen for growth and even dies in its presence. Obligate anaerobes will die when exposed to atmospheric levels of oxygen. Clostridium perfringens bacteria, which are commonly found in soil around the world, are obligate anaerobes. Infection of a wound by C. perfringens bacteria causes the disease gas gangrene. Obligate anaerobes use molecules other than oxygen as terminal electron acceptors.

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would not need oxygen for growth, sample A or sample C?
Answer: sample A

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would need oxygen for growth, sample A or sample C?
Answer: sample C

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample might die in presence of oxygen, sample A or sample C?
Answer: sample A

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample might not die in presence of oxygen, sample A or sample C?
Answer: sample C

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Would sample A die or thrive in presence of oxygen?
Answer: die

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Would sample C die or thrive in presence of oxygen?
Answer: thrive

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would use oxygen as terminal electron acceptor, sample A or sample C?
Answer: sample C

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would not use oxygen as terminal electron acceptor, sample A or sample C?
Answer: sample A

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would cause gas gangrene, sample B or sample C?
Answer: sample B

Situation: Microbiologist John is studying the anaerobic organism. He collected two samples of this organism, sample A and sample B. Sample A is an anaerobic organism, and sample B is specifically identified as the Clostridium perfringens obligate anaerobe. To compare these samples to other organisms he collected another microbe that is not an anaerobic organism. He labeled it as sample C.

Question: Which sample would not cause gas gangrene, sample B or sample C?
Answer: sample C