Prevalence of lameness and associated risk factors in Canadian Holstein-Friesian cows housed in freestall barns. The impact of parity and duration of biotin supplementation on white line disease lameness in dairy cattle. Lameness Prevalence and Risk Factors in Large Dairy Farms in Upstate New York. Model Development for the Prediction of Claw Horn Disruption Lesions Temporal associations between low body condition, lameness and milk yield in a UK dairy herd. Management practices associated with the bulk-milk prevalence of Staphylococcus aureus in Canadian dairy farms. The effect of lameness on the fertility of dairy cattle in a seasonally breeding pasture-based system. The effect of claw trimming on the hoof health of Swedish dairy cattle. Use of Extended Characteristics of Locomotion and Feeding Behavior for Automated Identification of Lame Dairy Cows Visual locomotion scoring in the first seventy days in milk: impact on pregnancy and survival. The development, implementation and testing of a lameness control programme based on HACCP principles and designed for heifers on dairy farms. Effect of supplemental fish meal on the lactation and reproductive performance of dairy cows. The effect of a progesterone releasing intravaginal device (PRID) on pregnancy risk to fixed-time insemination following diagnosis of non-pregnancy in dairy cows. Impact of hyperketonemia in early lactation dairy cows on health and production. A prospective cohort study of digital cushion and corium thickness. Part 1: Associations with body condition, lesion incidence, and proximity to calving. Electroacupuncture in the treatment of chronic lameness in horses and ponies: a controlled clinical trial. Does Dietary Mitigation of Enteric Methane Production Affect Rumen Function and Animal Productivity in Dairy Cows? An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production Evaluation of udder health parameters and risk factors for clinical mastitis in Dutch dairy herds in the context of a restricted antimicrobial usage policy. Effect of administration of a controlled-release monensin capsule on incidence of calving-related disorders, fertility, and milk yield in dairy cows. Management of dairy heifers and its relationships with the incidence of clinical mastitis The amount and type of dairy product intake and incident type 2 diabetes: results from the EPIC-InterAct Study. An evaluation of the effect of altering nutrition and nutritional strategies in early lactation on reproductive performance and estrous behavior of high-yielding Holstein-Friesian dairy cows. Do dairy products induce bronchoconstriction in adults with asthma?
Lameness is a severe welfare problem and a production-limiting disease in dairy farming . The objectives of this study were to determine prevalence of lameness and investigate cow- and herd-level factors associated with lameness in dairy cows housed in freestall barns in 3 Canadian provinces . A purposive sample of 40 Holstein-Friesian cows was selected from each of 141 dairy farms in Québec , Ontario , and Alberta . In total , 5,637 cows were scored once for lameness ( presence of limping when walking ) . Data collected included information on individual cows ( hock lesions , claw length , body condition score , parity , days in milk , and milk production ) , management practice s ( floor and stall cleaning routine , bedding routine , and footbath practice s ) , and facility design ( stall dimensions , stall base and bedding type , width of feed alley , flooring type , and slipperiness ) hypothesized to be risk factors for lameness . Multilevel mixed logistic regression models were constructed ( including farm as a r and om effect and province as a fixed effect ) . Herd-level lameness prevalence ranged from 0 to 69 % ( mean = 21 % ) . Lameness prevalence increased with increasing parity ; compared with first parity , cows in parity 2 , 3 , and ≥ 4 had 1.6 , 3.3 , and 4 times , respectively , higher odds of being lame . Furthermore , the odds of lameness were 1.6 times greater in cows with low body condition score ( ≤ 2.5 ) than in cows with a higher body condition score . In addition , injured hocks and overgrown claws were associated with 1.4- and 1.7-fold increased odds of being lame , respectively , whereas every 1 kg increase in daily milk production was associated with a 3 % decrease in the odds of being lame . Lameness prevalence was higher in herds with ≤ 100 cows , but lower in barns with a s and or dirt stall base , or with bedding ≥ 2 cm deep . Cows exposed to very slippery floors had 2 times the odds of being lame compared with cows exposed to nonslippery floors . We attributed the wide range of lameness prevalence to the great variability in facilities and management practice s among farms . Finally , we inferred that the prevalence of lameness could be decreased by improving management of multiparous , thin , or injured cows and by adopting management practice s intended to improve cow comfort , namely the floor 's slip resistance and the stall 's lying surface A field study was conducted to examine effects of oral biotin supplementation for up to 18 mo on risks of lameness in dairy cows . The study included a total of 900 cattle from five dairy farms in Gloucestershire , southwest U.K. , in a within-herd r and omized control trial . The data from this trial were used in this paper to investigate the impact of parity and duration of supplementation with oral biotin at 20 mg/d on white line disease ( WLD ) lameness . Analysis of the data indicated that WLD increased with increasing parity independent of biotin supplementation from approximately two cases per 100 cow years in primiparous cows to 15.5 cases per 100 cow years in all multiparous cows , but up to 47.7 cases per 100 cow years for cows in parities > or = 5 . Supplementation with biotin reduced WLD lameness by 45 % in multiparous cows down to 8.5 cases per 100 cow years , whereas the effect of biotin supplementation in primiparous cows was not significant . Although numerical reductions in WLD lameness were observed for shorter periods of supplementation , a supplementation length of at least 6 mo was required to significantly reduce the risk of WLD lameness in multiparous cows . The effect of biotin supplementation in reducing lameness has potential impact for both animal welfare and farm economics The main objectives of this prospect i ve cohort study were a ) to describe lameness prevalence at drying off in large high producing New York State herds based on visual locomotion score ( VLS ) and identify potential cow and herd level risk factors , and b ) to develop a model that will predict the probability of a cow developing claw horn disruption lesions ( CHDL ) in the subsequent lactation using cow level variables collected at drying off and /or available from farm management software . Data were collected from 23 large commercial dairy farms located in upstate New York . A total of 7,687 dry cows , that were less than 265 days in gestation , were enrolled in the study . Farms were visited between May 2012 and March 2013 , and cows were assessed for body condition score ( BCS ) and VLS . Data on the CHDL events recorded by the farm employees were extracted from the Dairy-Comp 305 data base , as well as information regarding the studied cows ’ health events , milk production , and reproductive records throughout the previous and subsequent lactation period . Univariable analyses and mixed multivariable logistic regression models were used to analyse the data at the cow level . The overall average prevalence of lameness ( VLS > 2 ) at drying off was 14 % . Lactation group , previous CHDL , mature equivalent 305-d milk yield ( ME305 ) , season , BCS at drying off and sire PTA for strength were all significantly associated with lameness at the drying off ( cow-level ) . Lameness at drying off was associated with CHDL incidence in the subsequent lactation , as well as lactation group , previous CHDL and ME305 . These risk factors for CHDL in the subsequent lactation were included in our predictive model and adjusted predicted probabilities for CHDL were calculated for all studied cows . ROC analysis identified an optimum cut-off point for these probabilities and using this cut-off point we could predict CHDL incidence in the subsequent lactation with an overall specificity of 75 % and sensitivity of 59 % . Using this approach , we would have detected 33 % of the studied population as being at risk , eventually identifying 59 % of future CHDL cases . Our predictive model could help dairy producers focusing their efforts on CHDL reduction by implementing aggressive preventive measures for high risk cows Previous work has hypothesised that cows in low body condition become lame . We tested this in a prospect i ve longitudinal study . Body condition score ( BCS ) , causes of lameness and milk yield were collected from a 600-cow herd over 44-months . Mixed effect binomial models and a continuous outcome model were used to investigate the associations between lameness , BCS and milk yield . In total , 14,320 risk periods were obtained from 1137 cows . There were 1510 lameness treatments : the most common causes of lameness were sole ulcer ( SU ) ( 39 % ) , sole haemorrhage ( SH ) ( 13 % ) , digital dermatitis ( DD ) ( 10 % ) and white line disease ( WLD ) ( 8 % ) . These varied by year and year quarter . Body condition was scored at 60-day intervals . BCS ranged from 1 to 5 with a mean of 2.5 , scores were higher in very early lactation but varied widely throughout lactation ; approximately 45 % of scores were < 2.5 . The key finding was that BCS<2.5 was associated with an increased risk of treatment for lameness in the following 0 - 2 months and > 2 - 4 months for all causes of lameness and also specifically for SU/WLD lameness . BCS<2.5 was associated with an increased risk of treatment for SH in the following 0 - 2 months but not > 2 - 4 months . There was no such association with DD . All lameness , SU/WLD , SH and DD were significantly more likely to occur in cows that had been lame previously , but the effect of BCS was present even when all repeat cases of lameness were excluded from the analysis . Milk yield was significantly higher and fell in the month before treatment in cows lame with SU/WLD but it was not significantly higher for cows that were treated for DD compared with non-lame cows . These findings support the hypothesis that low BCS contributes to the development of horn related claw lameness but not infectious claw diseases in dairy cows . One link between low BCS and lameness is a thin digital cushion which has been proposed as a trigger for claw horn disease . Cows with BCS 2 produced more milk than cows with BCS 2.5 , however , this was only approximately 100 kg difference in yield over a 305-day lactation . Given the increased risk of lameness in cows with BCS 2 , the direct costs of lameness and the small variability in milk yield by BCS , preventing cows from falling to BCS<2.5 would improve cow welfare and be economically beneficial When design ing mastitis-prevention and control programs , it is important to know the level of adoption of mastitis-prevention management practice s and control programs and the herd-level prevalence of contagious mastitis pathogens . Our objectives were to estimate : ( 1 ) adoption of recommended mastitis-preventive management on Canadian dairy farms ; ( 2 ) herd-level prevalence of contagious mastitis pathogens on Canadian dairy farms ; and ( 3 ) associations of certain management practice s with the isolation of Staphylococcus aureus from the bulk tank milk from Canadian dairy farms . In total , 226 farms participating in dairy herd improvement milk recording were r and omly selected . All participating farms in British Columbia had free-stall barns and 85 % of farms in Québec had tie-stall barns .  Post-milking teat disinfection was practised on 96 % of the farms and 72 % had implemented blanket dry-cow treatment . Weighted and province-stratified prevalence of Streptococcus agalactiae and Staph . aureus in bulk tank milk was 4 % ( 95 % confidence interval : 0 - 12 % ) and 74 % ( 95 % confidence interval : 61 - 86 % ) , respectively . Highest Staph . aureus prevalence was found in Nova Scotia ( 91 % ) and lowest prevalence in British Columbia ( 38 % ) . No Mycoplasma spp . were isolated , but detection of Mycoplasma spp . could have been hampered by the frozen shipment and storage of the milk sample s. Management practice s associated with a lower probability of isolating Staph . aureus were blanket dry-cow treatment and believing that a nutritionist is important in mastitis data review . Having the milking equipment checked by an independent technician at least once a year and rubber mats or mattresses in the free-stall barns were associated with an increased probability of isolating Staph . aureus from the bulk tank . Most of Canadian dairy farms adopted important mastitis-prevention practice s , such as post-milking teat disinfection and drying off all cows with antibiotics ; however , improvements can still be made . A few management practice s were associated with the prevalence of Staph . aureus in bulk tank milk , such as dry-cow treatment and barn type for the lactating cows . Further work has to be carried out to investigate to what extent the associations are causal The effect of lameness on the fertility of dairy cattle is well recognized . But , the effect of lameness on the fertility of seasonally breeding cattle in pasture-based systems is less well characterized . This prospect i ve cohort study of 463 cows on 1 farm in the lower North Isl and of New Zeal and was design ed to assess the effect of clinical lameness , as identified by farm staff , on the hazard of conception after the planned start-of-mating date . A Cox proportional hazards model with time-varying covariates was used . After controlling for the effect of parity , breed , body weight at calving , and calving-to-planned start of mating interval , the daily hazard of conception for cows identified as lame was 0.78 ( 95 % confidence interval : 0.68 - 0.86 ) compared with non-lame cows . Lame cows took 12 d longer to get pregnant compared with their non-lame counterparts A 2-year experiment on the effect of claw trimming on hoof health was performed in 77 Swedish dairy herds ( 3444 dairy cattle ) selected on herd size , breed composition and membership in the official milk-recording scheme . In the autumn , cows within each herd were blocked according to breed , parity and stage of lactation and allocated to two treatments : autumn trimming ( AT ) or no autumn trimming ( NAT ) . Outcome variables were claw measurements and prevalence of hoof lesions and lameness ( measured at spring trimming ) and the need for hoof treatments between scheduled trimmings . At spring trimming , NAT cows had longer and shallower claws than AT cows . The average net growth of the toe wall was greater for AT than for NAT cattle , with a marked variation between housing systems . Most hoof lesions present at AT had disappeared at the subsequent spring trimming . Controlled for clustering by herd-within-year and for the effects of individual- and herd-level covariates , AT cattle at spring trimmings had significantly lower odds of lameness ( OR=0.66 ) and of haemorrhages of the sole or white-line ( OR=0.86 ) , sole ulcer ( OR=0.59 ) and white-line fissure or double sole (OR=0.71)-but not of moderate-to-severe heel-horn erosion or dermatitis ( OR=0.96 ) . Acute hoof treatments between claw trimmings were more common in the NAT group ( OR=2.02 ) This study was carried out to detect differences in locomotion and feeding behavior in lame ( group L ; n = 41 ; gait score ≥ 2.5 ) and non-lame ( group C ; n = 12 ; gait score ≤ 2 ) multiparous Holstein cows in a cross-sectional study design . A model for automatic lameness detection was created , using data from accelerometers attached to the hind limbs and noseb and sensors attached to the head . Each cow ’s gait was videotaped and scored on a 5-point scale before and after a period of 3 consecutive days of behavioral data recording . The mean value of 3 independent experienced observers was taken as a definite gait score and considered to be the gold st and ard . For statistical analysis , data from the noseb and sensor and one of two accelerometers per cow ( r and omly selected ) of 2 out of 3 r and omly selected days was used . For comparison between group L and group C , the T-test , the Aspin-Welch Test and the Wilcoxon Test were used . The sensitivity and specificity for lameness detection was determined with logistic regression and ROC- analysis . Group L compared to group C had significantly lower eating and ruminating time , fewer eating chews , ruminating chews and ruminating boluses , longer lying time and lying bout duration , lower st and ing time , fewer st and ing and walking bouts , fewer , slower and shorter strides and a lower walking speed . The model considering the number of st and ing bouts and walking speed was the best predictor of cows being lame with a sensitivity of 90.2 % and specificity of 91.7 % . Sensitivity and specificity of the lameness detection model were considered to be very high , even without the use of halter data . It was concluded that under the conditions of the study farm , accelerometer data were suitable for accurately distinguishing between lame and non-lame dairy cows , even in cases of slight lameness with a gait score of 2.5 Our hypotheses were that cows classified as lame during the first 70 d in milk have more days from calving to conception and a greater hazard of dying or being culled compared with cows that were not classified as lame . Our objective was to estimate the detrimental effects of lameness on calving-to-conception interval and hazard of dying or being culled in lactating Holstein cows . Data were collected from 5 dairy farms located in upstate New York from November 2004 to June 2006 . The design was a prospect i ve observational cohort study . Cows were assigned a  visual locomotion score ( VLS ) using a 5-point scale : 1 = normal , 2 = presence of a slightly asymmetric gait , 3 = the cow clearly favored 1 or more limbs ( moderately lame ) , 4 = severely lame , to 5 = extremely lame ( nonweight-bearing lame ) . In total 1,799 cows were enrolled . In 2 alternative categorizations , cows were considered lame if at least 1 VLS was > or =3 during the first 70 d in milk , and if at least 1 VLS was > or = 4 for the same period they were considered lame . Lameness ( VLS > or =3 ) was detected at least once in 26.5 , 54.2 , 33.9 , 51.8 , and 39.3 % of all cows in farms 1 to 5 , respectively . The hazard ratio of being detected pregnant was 0.85 for lame cows ( VLS > or =3 ) vs. nonlame cows ; hence , lame cows were at a 15 % lower risk of pregnancy than nonlame cows . When lameness was redefined as VLS > or = 4 , the hazard ratio of been detected pregnant was 0.76 for lame cows vs. cows with VLS < 4 . Lameness increased the hazard ratio of culling/death , 1.45 and 1.74 for VLS > or =3 and VLS > or = 4 , respectively , vs. cows with VLS <3 and VLS < 4 , respectively . In summary , lameness significantly decreased the hazard of pregnancy and increased the hazard of culling/death . The detrimental effects were amplified when considering only severely lame and non-weight-bearing cows This paper describes the development and testing of a lameness control programme ( LCP ) for heifers on dairy farms . The LCP , which is based on the analysis of hazards and critical control points ( HACCP ) , was tested via a r and omised intervention study on 60 farms . Tangible hazards for each farm were identified , allotted to 11 categories of proximate hazard and scored on each farm to quantify the risks presented by each hazard . Feet were inspected for signs of claw horn disease and infection , such as digital dermatitis . Intervention was generally ineffective , primarily through failure to implement the LCP . However , retrospective analysis was able to demonstrate highly significant associations between risks attached to proximate hazards and probabilities of lameness and foot lesions , allowing the severity of these hazards to be ranked . The most significant proximate hazards of environmental origin were prolonged st and ing on concrete , st and ing in wet slurry and factors that cause claw trauma . The most severe proximate hazards however were those associated with failures of management , especially poor claw condition and inadequate foot care . Overall farm risks ( OFR ) were estimated by summing the products of the generic severity for proximate hazards with on-farm risks . Changes in OFR were significantly related to changes in outcome ( lameness and lesions ) The influence of high protein diets ( 21 % CP , DM basis ) , containing varied percentages of RUP , on lactation performance and fertility  was evaluated . Sixty-two Holstein cows ( 65 % multiparous ) were blocked by age and r and omly assigned to a 2 x 2 x 2 factorial design from d 12 to 125 postpartum . Factor 1 was 0 or 3.5 % fish meal diet , factor 2 was location ( Calan door versus free stall ) , and factor 3 was parity ( first versus second or later ) . The soybean meal diet consisted of alfalfa hay , corn silage , barley , and soybean meal . The fish meal diet contained 3.5 % fish meal ( ruminant grade menhaden ) that replaced a portion of the soybean meal . Cows fed the fish meal diet ( 40 % RUP ) had DMI , BW , and body condition similar to those of cows offered the soybean meal diet ( 34 % RUP ) . Cows receiving the fish meal supplement tended to have higher milk protein production throughout the trial , higher milk production during the first 6 wk , and significantly lower ruminal ammonia concentrations than cows receiving the soybean meal diet . Differences in reproductive performance were not significant except for a diet by housing location interaction for conception rates from first AI The objective was to compare the probability of pregnancy after fixed-time insemination in cows diagnosed as non-pregnant and re-inseminated following the Ovsynch protocol , with or without exogenous progesterone . Cows ( n=415 ) used in this study originated from 25 farms . Upon diagnosis of non-pregnancy between 30 and 60 days after AI , cows were r and omly assigned to receive either a progesterone releasing intravaginal device ( PRID ; n=208 ) or a placebo intravaginal device ( PID ; n=207 ) . All cows received GnRH at enrollment ( Day 0 ) , PGF(2alpha ) concurrent with intravaginal device removal 7 days later , GnRH on Day 9 and fixed-time insemination 16h later ( Day 10 ) . Cows observed in estrus prior to Day 7 , had the device removed and were inseminated . Ovaries were examined by transrectal palpation at the time of enrollment and the prominent structures were assessed and recorded . Body condition score , lameness status , interval from previous insemination , and times bred at enrollment were recorded . At intravaginal device removal , the occurrence and intensity of vaginitis was determined according to the amount of debris on the device . Overall , the intravaginal device retention rate was 91 % . A total of 5.2 % of PID-treated cows and 2.9 % of PRID-treated cows were detected in estrus within the 7 days treatment period . Pregnancy status was diagnosed between 30 and 56 days after insemination and all cows were followed for a minimum of 150 days after enrollment . Approximately 28 % of cows had evidence of mild vaginitis in response to the intravaginal device , whereas 6 % of cows had copious debris associated with the intravaginal device at removal . The probability of pregnancy after fixed-time insemination was 43.8 % versus 34.9 % in PRID-treated versus PID-treated animals . Exogenous progesterone provided through an intravaginal device to non-pregnant cows that had not displayed estrus improved the probability of pregnancy after fixed-time AI Data from 1,010 lactating lactating , predominately component-fed Holstein cattle from 25 predominately tie-stall dairy farms in southwest Ontario were used to identify objective thresholds for defining hyperketonemia in lactating dairy cattle based on negative impacts on cow health , milk production , or both . Serum sample s obtained during wk 1 and 2 postpartum and analyzed for beta-hydroxybutyrate ( BHBA ) concentrations that were used in analysis . Data were time-ordered so that the serum sample s were obtained at least 1 d before the disease or milk recording events . Serum BHBA cutpoints were constructed at 200 micromol/L intervals between 600 and 2,000 micromol/L. Critical cutpoints for the health analysis were determined based on the threshold having the greatest sum of sensitivity and specificity for predicting the disease occurrence . For the production outcomes , models for first test day milk yield , milk fat , and milk protein percentage were constructed including covariates of parity , precalving body condition score , season of calving , test day linear score , and the r and om effect of herd . Each cutpoint was tested in these models to determine the threshold with the greatest impact and least risk of a type 1 error . Serum BHBA concentrations at or above 1,200 micromol/L in the first week following calving were associated with increased risks of subsequent displaced abomasum [ odds ratio ( OR ) = 2.60 ] and metritis ( OR = 3.35 ) , whereas the critical threshold of BHBA in wk 2 postpartum on the risk of abomasal displacement was > or=1,800 micromol/L ( OR = 6.22 ) . The best threshold for predicting subsequent risk of clinical ketosis from serum obtained during wk 1 and wk 2 postpartum was 1,400 micromol/L of BHBA ( OR = 4.25 and 5.98 , respectively ) . There was no association between clinical mastitis and elevated serum BHBA in wk 1 or 2 postpartum , and there was no association between wk 2 BHBA and risk of metritis . Greater serum BHBA measured during the first and second week postcalving were associated with less milk yield , greater milk fat percentage , and less milk protein percentage on the first Dairy Herd Improvement test day of lactation . Impacts on first Dairy Herd Improvement test milk yield began at BHBA > or=1,200 micromol/L for wk 1 sample s and > or=1,400 micromol/L for wk 2 sample s. The greatest impact on yield occurred at 1,400 micromol/L ( -1.88 kg/d ) and 2,000 micromol/L ( -3.3 kg/d ) for sera from the first and second week postcalving , respectively . Hyperketonemia can be defined at 1,400 micromol/L of BHBA and in the first 2 wk postpartum increases disease risk and results in substantial loss of milk yield in early lactation Claw horn disruption lesions ( CHDL ) are a major cause of lameness in dairy cattle and are likely a result of excessive forces being applied to the germinal epithelium that produces the claw horn . The digital cushion is a connective tissue structure , containing depots of adipose tissue , that sits beneath the distal phalanx and has been shown to be thicker in fatter cows . Body condition score ( BCS ) loss is a risk factor for CHDL , and one possible explanation is that fat is mobilized from the digital cushion during negative energy balance , causing the digital cushion to thin and lose force-dissipating capacity , leading to disruption of claw horn growth . This prospect i ve cohort study investigated the association between measures of body fat and sole soft tissue ( SST ) thickness ( a combined measure of the corium and digital cushion beneath the distal phalanx ) in a longitudinal manner . The  SST  of 179 cows in 2 high-yielding dairy herds were measured at 5 assessment points between 8 wk before and 35 wk postcalving . The BCS , back fat thickness ( BFT ) , and lesion incidence were recorded . Data were analyzed in a 4-level mixed effects regression model , with the outcome being SST thickness beneath the flexor tuberosity of the distal phalanx . Data from 827 assessment points were available for analysis . The overall mean of SST was 4.99 mm ( st and ard deviation : 0.95 ) . The SST was thickest 8 wk before calving ( 5.22 mm , st and ard deviation : 0.91 ) and thinnest 1 wk postcalving ( 4.68 mm , st and ard deviation : 0.87 ) , suggesting an effect of calving on SST . The BFT was positively correlated with SST in the model with a small effect size ( a 10 mm decrease in BFT corresponded with a 0.13 mm decrease in SST ) , yet the nadir of BFT was 11.0 mm at 9 to 17 wk postcalving ( when SST was ∼4.95 mm ) , rather than occurring with the nadir of SST immediately after calving . The SST also varied with other variables [ e.g. , cows that developed a sole ulcer or severe sole hemorrhage during the study had thinner SST ( -0.24 mm ) ] , except when a sole ulcer was present , when it was thicker ( + 0.53 mm ) . Cows that developed lesions had a thinner digital cushion before the lesion occurrence , which became thickened with sole ulcer presence , perhaps representing inflammation . Furthermore , although BFT was correlated with SST over time , SST may also have been influenced by other factors such as integrity of the suspensory apparatus , which could have a major effect on CHDL . Measures of body fat likely contributed to having thin SST , but other factors including calving , herd , and lesion presence also had an effect Electroacupuncture was used to treat lameness in horses and ponies with chronic laminitis ( n = 10 ) or navicular disease ( n = 10 ) . A clinical trial was conducted with r and om allocation of equal numbers of animals to control and treatment groups . Acupuncture was performed three times per week for four consecutive weeks . The degree of lameness was assessed by 1 ) a grading scheme , 2 ) measurement of stride lengths and 3 ) analysis of weight distribution using a force plate . Although seven out of ten animals with chronic laminitis improved clinical ly during the trial , there were no statistically significant differences between treatment and control groups . Six out of ten horses with navicular disease improved , but there were no significant differences between treatment and control groups It has been suggested that the rumen microbiome and rumen function might be disrupted if methane production in the rumen is decreased . Furthermore concerns have been voiced that geography and management might influence the underlying microbial population and hence the response of the rumen to mitigation strategies . Here we report the effect of the dietary additives : linseed oil and nitrate on methane emissions , rumen fermentation , and the rumen microbiome in two experiments from New Zeal and ( Dairy 1 ) and the UK ( Dairy 2 ) . Dairy 1 was a r and omized block design with 18 multiparous lactating cows . Dairy 2 was a complete replicated 3 x 3 Latin Square using 6 rumen cannulated , lactating dairy cows . Treatments consisted of a control total mixed ration ( TMR ) , supplementation with linseed oil ( 4 % of feed DM ) and supplementation with nitrate ( 2 % of feed DM ) in both experiments . Methane emissions were measured in open circuit respiration chambers and rumen sample s were analyzed for rumen fermentation parameters and microbial population structure using qPCR and next generation sequencing ( NGS ) . Supplementation with nitrate , but not linseed oil , decreased methane yield ( g/kg DMI ; P<0.02 ) and increased hydrogen ( P<0.03 ) emissions in both experiments . Furthermore , the effect of nitrate on gaseous emissions was accompanied by an increased rumen acetate to propionate ratio and consistent changes in the rumen microbial population s including a decreased abundance of the main genus Prevotella and a decrease in archaeal mcrA ( log10 copies/ g rumen DM content ) . These results demonstrate that methane emissions can be significantly decreased with nitrate supplementation with only minor , but consistent , effects on the rumen microbial population and its function , with no evidence that the response to dietary additives differed due to geography and different underlying microbial population Significance Methane from enteric fermentation in the ruminant digestive system is a major contributor to anthropogenic greenhouse gas emissions in the United States and worldwide . Methane is also a net loss of feed energy to the animal . This study was undertaken to investigate the effect of a methane inhibitor on enteric methane emissions from lactating dairy cows . The experiment demonstrated that , under industry-relevant conditions , the inhibitor persistently decreased by 30 % enteric methane emissions , without negatively affecting animal productivity . The spared methane energy was partially used for tissue synthesis , which led to a greater body weight gain by the inhibitor-treated cows . If adopted , this mitigation practice could lead to a substantial reduction of greenhouse gas emissions from the ruminant livestock sector . A quarter of all anthropogenic methane emissions in the United States are from enteric fermentation , primarily from ruminant livestock . This study was undertaken to test the effect of a methane inhibitor , 3-nitrooxypropanol ( 3NOP ) , on enteric methane emission in lactating Holstein cows . An experiment was conducted using 48 cows in a r and omized block design with a 2-wk covariate period and a 12-wk data collection period . Feed intake , milk production , and fiber digestibility were not affected by the inhibitor . Milk protein and lactose yields were increased by 3NOP . Rumen methane emission was linearly decreased by 3NOP , averaging about 30 % lower than the control . Methane emission per unit of feed dry matter intake or per unit of energy-corrected milk were also about 30 % less for the 3NOP-treated cows . On average , the body weight gain of 3NOP-treated cows was 80 % greater than control cows during the 12-wk experiment . The experiment demonstrated that the methane inhibitor 3NOP , applied at 40 to 80 mg/kg feed dry matter , decreased methane emissions from high-producing dairy cows by 30 % and increased body weight gain without negatively affecting feed intake or milk production and composition . The inhibitory effect persisted over 12 wk of treatment , thus offering an effective methane mitigation practice for the livestock industries Recently , many changes have been implemented in Dutch dairy herds . Herd sizes have increased and antimicrobial use has been reduced . Certain types of antimicrobials can only be used in specific circumstances , and the preventive use of antimicrobials in dry cows is prohibited . The aim of this study was to quantify clinical mastitis ( CM ) , sub clinical mastitis ( SCM ) , and risk factors associated with CM in Dutch dairy herds in 2013 , in the context of these changes . For this study , 240 dairy herds were r and omly selected from farms that participated in test-day milk recording , used a conventional milking system , and agreed to participate in the study . Eventually , 233 Dutch dairy farmers had complete records of CM in their herds in 2013 and 224 of these farmers completed a question naire on management factors potentially associated with CM . All participating farmers gave consent to use their routinely collected herd data such as test-day records and cow identification and registration data . Clinical and sub clinical mastitis incidence rate ( CMI and SCMI , respectively ) per 100 cows per year , sub clinical mastitis prevalence , and average bulk tank milk somatic cell count were obtained for 2013 . The risk factor analysis was conducted using a generalized linear model with a log link function and a negative binomial distribution on herd level in Stata 13.1 . A median CMI of 28.6 per 100 cows at risk per year , SCMI of 70.1 per 100 cows at risk per year , SCM prevalence of 15.8 % , and bulk tank milk somatic cell count of 171 × 10(3 ) cells/mL were observed in 2013 . Factors that were significantly associated with a higher CMI were cleaning slatted floors only once per day compared with more than 4 times a day ( i.e. , mechanical ) , a higher percentage of Holstein Friesian cows present in the herd , treating less than 50 % of the cows with CM with antimicrobials , postmilking teat disinfection , and treatment of cows with elevated somatic cell count with antimicrobials . The results of this study indicated that udder health had not deteriorated compared with udder health in previous Dutch studies where herd sizes were somewhat smaller and before the restrictions in antimicrobial use . Several of the risk factors that were found can be influenced by the farmer and can prevent the occurrence of CMI . Still , when cases of CM occur , treatment with antimicrobials might be necessary to cure the CM case and is beneficial for the overall udder health in the herd OBJECTIVE To determine the effect of a controlled-release monensin capsule administered at cessation of lactation on incidence of calving-related disorders , fertility , and milk yield in dairy cows . ANIMALS 290 dairy cows treated with monensin and 290 untreated control cows . PROCEDURE Treated cows received a capsule that released monensin at 335 mg/d for 95 days . Incidence of calving-related disorders ; daily milk yield up to 20 days postpartum ; test-day milk yield , fat , protein , and mature-equivalent 305-day milk production ; and body condition score at calving were determined . Reproductive variables were conception rate at first service , pregnancy rate , and calving-to-conception interval . RESULTS Cows treated with monensin were 2.1 times as likely to develop dystocia and 0.8 times as likely to develop metritis as control cows . For milk yield , there was an interaction of treatment X time X parity . In multiparous cows , monensin significantly improved milk yield at test days 4 and 7 . In addition , monensin increased body condition score at calving . CONCLUSIONS AND CLINICAL RELEVANCE Despite increasing the likelihood of developing dystocia and metritis , administration of monensin improved the lactational performance of multiparous cows and may be a promising additive for use at the time of cessation of lactation Abstract AIM : To describe aspects of management of dairy heifers before calving and determine risk factors for clinical mastitis postpartum in heifers , at the herd level , under pasture-based management systems in the Waikato and Taranaki regions of New Zeal and . METHODS : Dairy herdowners ( n=578 ) provided information via a prospect i ve survey about their practice s for rearing heifers and management of mastitis . A proportion of herdowners ( n=250 ) subsequently provided data on the cases of clinical mastitis in their herds , including the date , cow identification , age and quarter affected from cases occurring in the 4 months after the planned start of calving ( PSC ) in the subsequent lactation . The relationship between management factors and the proportion of heifers diagnosed with clinical mastitis within a herd was examined using bivariate and multivariate analyses . RESULTS : The herd average percentage of heifers with clinical mastitis was 13.6 ( 95 % confidence interval (CI)=12.3–14.9)% , and multiparous cows with clinical mastitis was 9.0 ( 95 % CI=8.2–9.8)% in the first 4 months of lactation . There were positive relationships between the proportion of heifers with clinical mastitis and average milk production per cow ( kg milksolids/ lactation ; p<0.001 ) , number of cows milked per labour unit ( p=0.003 ) , stocking rate ( < > 3.30 cows/ha ; p=0.002 ) , and incidence of clinical mastitis in multiparous cows ( % /120 days ; p<0.04 ) , in the final multivariate model . The proportion of heifers with clinical mastitis per herd was lower in herds that milked their lactating cows in multiple groups ( p=0.02 ) . CONCLUSIONS : The risk of clinical mastitis  in heifers was significantly associated with management practice s. It may be possible to reduce the incidence of clinical mastitis in heifers by modification of management practice s at the herd level , and further studies are required to investigate this BACKGROUND Dairy product intake may be inversely associated with risk of type 2 diabetes  , but the evidence is inconclusive for total dairy products and sparse for types of dairy products . OBJECTIVE The objective was to investigate the prospect i ve association of total dairy products and different dairy subtypes with  incidence of diabetes in population s with marked variation of intake of these food groups . DESIGN A nested case-cohort within 8 European countries of the European Prospect i ve Investigation into Cancer and Nutrition Study ( n = 340,234 ; 3.99 million person-years of follow-up ) included a r and om subcohort ( n = 16,835 ) and incident diabetes cases ( n = 12,403 ) .  Baseline dairy product intake was assessed by using dietary question naires . Country-specific Prentice-weighted Cox regression HRs were calculated and pooled by using a r and om-effects meta- analysis . RESULTS Intake of total dairy products was not associated with diabetes ( HR for the comparison of the highest with the lowest quintile of total dairy products : 1.01 ; 95 % CI : 0.83 , 1.34 ; P-trend = 0.92 ) in an analysis adjusted for age , sex , BMI , diabetes risk factors , education , and dietary factors . Of the dairy subtypes , cheese intake tended to have an inverse association with diabetes ( HR : 0.88 ; 95 % CI : 0.76 , 1.02 ; P-trend = 0.01 ) , and a higher combined intake of fermented dairy products ( cheese , yogurt , and thick fermented milk ) was inversely associated with diabetes ( HR : 0.88 ; 95 % CI : 0.78 , 0.99 ; P-trend = 0.02 ) in adjusted analyses that compared extreme quintiles . CONCLUSIONS This large prospect i ve study found no association between total dairy product intake and diabetes risk . An inverse association of cheese intake and combined fermented dairy product intake with diabetes is suggested , which merits further study Reproductive performance in the high-yielding dairy cow has severely decreased in the last 40 yr . The aim of this study was to compare the effectiveness of 4 nutritional strategies in improving the reproductive performance of high-yielding dairy cows . It was hypothesized that offering cows a high-starch ration in early lactation would enhance the onset of luteal activity , and that decreasing the severity of negative energy balance in the early postcalving period would improve reproductive parameters . Nutritional regimens aim ed at improving fertility were applied to 96 Holstein-Friesian dairy animals . Upon calving , animals were allocated in a balanced manner to one of 4 dietary treatments . Primiparous animals were balanced according to live weight , body condition score and calving date . Multiparous animals were balanced according to parity , previous lactation milk yield , liveweight , body condition score and calving date . Treatment 1 was based on an industry best practice diet ( control ) to contain 170 g of crude protein/kg of dry matter . Treatment 2 was an individual cow feeding strategy , whereby the energy balance ( EB ) of individual animals was managed so as to achieve a predetermined target daily EB profile ( ±10 MJ/d ) . Treatment 3 was a high-starch/high-fat combination treatment , whereby an insulinogenic ( high-starch ) diet was offered in early lactation to encourage cyclicity and followed by a lipogenic ( low-starch , high-fat ) diet to promote embryo development . Treatment 4 was a low-protein diet , containing 140 g of crude protein/kg of dry matter , supplemented with protected methionine at an inclusion level of 40 g per animal per day . The nutritional strategies implemented in this study had no statistically significant effects on cow fertility measures , which included the onset of luteal activity , conception rate , in-calf rate , and the incidence of atypical cycles . The individual cow feeding strategy improved EB in early lactation but had no benefit on conception rate to first insemination . However , conception rate to second insemination , 100-d pregnancy rate ( from the commencement of breeding ) , and overall pregnancy rate tended to be higher in this group . The high-starch/high-fat treatment tended to decrease the proportion of delayed ovulations and increase the proportion of animals cycling by d 50 postcalving . Animals that failed to conceive to first insemination had a significantly longer luteal phase in the first cycle postpartum and a longer inter-ovulatory interval in the second cycle postpartum . With regards to estrous behavior , results indicate that as the size of the sexually active group increased , the intensity of estrus and the expression of mounting or attempting to mount another cow also increased . Furthermore , cows that became pregnant displayed more intense estrous behavior than cows that failed to become pregnant BACKGROUND Dairy products have often been implicated as a cause of exacerbation of asthma , but there is little scientific evidence to support this hypothesis . OBJECTIVE We sought to determine whether dairy products induce bronchoconstriction in a group of adults with asthma . METHODS Twenty subjects with asthma ( 13 women and 7 men ) were recruited from respondents who had previously completed a food and asthma question naire . Ten subjects perceived that their asthma became worse with ingestion of dairy products ( positive perceivers ) , whereas ten were negative perceivers . None of the subjects had positive skin prick test results with cow 's milk . The study was a r and omized , cross-over , double-blind , placebo-controlled trial . Subjects complied with a dairy-free diet throughout the study . The active challenge was a single-dose drink equivalent to 300 ml of cow 's milk . A positive reaction was defined as a 15 % reduction in both FEV1 and peak expiratory flow ( PEF ) on the active challenge day compared with results obtained at the same time on the placebo day . RESULTS For both FEV1 and PEF there were no statistically significantly differences in group means between active challenge and placebo challenge , between sequence of administration , or between perceptions . Nine subjects showed FEV1 or PEF changes that were greater than 15 % of baseline values : four patients showed changes after both active and placebo treatment ; two after treatment with placebo only ; and three after active treatment alone . Of the latter group , two subjects showed changes only in PEF , and when one of these subjects underwent a further detailed study , no asthmatic reaction could be demonstrated . CONCLUSION It is unlikely that dairy products have a specific bronchoconstrictor effect in most patients with asthma , regardless of their perception
A higher risk of being lame was found for the presence of claw overgrowth , the first 120 days in milk , larger herd sizes , and increasing parity .
Background Lameness in dairy cows has been an ongoing concern of great relevance to animal welfare and productivity in modern dairy production .
Many studies have examined associations between various factors related to housing , management , and the individual animal and the occurrence of lameness .
The objective of this systematic review was to answer the research question “ what are risk factors associated with lameness in dairy cows that are housed in free stall barns or tie stall facilities ” .
Furthermore , we performed a synthesis of current evidence on certain risk factors by means of a meta- analysis to illustrate the strength of their association with bovine lameness .