In Vivo and In Vitro Effects of Chlorhexidine Pretreatment on Immediate and Aged Dentin Bond Strengths. Bonding Performance of a Multimode Adhesive to Artificially-induced Caries-affected Primary Dentin. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Effect of phosphoric acid etching prior to self-etching primer application on adhesion of resin composite to enamel and dentin. Bonding of simplified adhesive systems to caries-affected dentin of primary teeth. Effect of caries removal techniques on the bond strength of adhesives to caries-affected primary dentin in vitro. Adhesive performance of dentin bonding agents applied in vivo and in vitro. Effect of intrapulpal pressure and dentin depth. Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin Bond strength of self-etching primer and total-etch adhesive systems to primary dentin. Does the method of caries induction influence the bond strength to dentin of primary teeth? Effect of haemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems. Effect of silver diamine fluoride on microtensile bond strength to dentin. Bond strength of two adhesive systems to primary and permanent enamel. Bond strength of adhesive resin cement with different adhesive systems Primary dentin etching time, bond strength and ultra-structure characterization of dentin surfaces. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin Phosphoric acid-etching promotes bond strength and formation of acid-base resistant zone on enamel. Microtensile bond strength of a total-etching versus self-etching adhesive to caries-affected and intact dentin in primary teeth. Long-term dentin retention of etch-and-rinse and self-etch adhesives and a resin-modified glass ionomer cement in non-carious cervical lesions. Tensile bond strength to and SEM evaluation of ground and intact enamel surfaces. Effect of resin cement system and root region on the push-out bond strength of a translucent fiber post. Total-etch versus self-etch adhesive: effect on postoperative sensitivity. Bond strength of a mild self-etch adhesive with and without prior acid-etching.
This study evaluated the effect of 2 % chlorhexidine ( CHX ) pretreatment of dentin on the immediate and aged microtensile bond strength ( μTBS ) of different adhesives to dentin in vivo and in vitro . Class I cavities were prepared in 80 caries-free human third molars of 40 patients in a split-mouth fashion . In each tooth pair , one tooth received 2 % CHX pretreatment after which both teeth were r and omly assigned to one of the following groups with respect to the type of adhesive system applied : Adper Single Bond 2 ( etch- and -rinse ) , Clearfil SE Bond ( two-step self-etch ) , Clearfil S(3 ) Bond ( one-step self-etch ) , and Adper Prompt-L-Pop ( all-in-one self-etch ) . The teeth were restored with resin composite and extracted for μTBS testing either immediately or after six months in function . In vitro specimen pairs were prepared as with the clinical protocol in intact , freshly extracted human molars , and thereafter , subjected to testing immediately or after 5000 × thermocycling . Data were analyzed with four-way analysis of variance ( ANOVA ) . Bonferroni test was utilized for pair-wise comparisons . The immediate bond strength values were significantly higher than " aged " ones for all tested adhesives ( p=0.00 ) . The in vitro immediate bond strength values were statistically higher than in vivo bond strength values ( p<0.05 ) . While the bond strength of in vitro aged , CHX-treated sample s were higher than their in vivo counterparts ( p<0.05 ) , no difference was observed in non-CHX treated groups ( p>0.05 ) . In the absence of CHX pretreatment , all adhesives showed significantly higher immediate bond strength values than CHX-treated groups , while all " aged " , non-pretreated adhesives exhibited significantly lower bond strength values ( both p<0.05 ) . By contrast , chlorhexidine pretreatment result ed in significantly higher aged bond strengths , regardless of the adhesive system and testing condition . Aging-associated decline in dentin bond strength of etch- and rinse and self-etch adhesives can be counteracted by chlorhexidine application PURPOSE To investigate the bonding of a new universal adhesive applied using different etching strategies on sound and caries-affected dentin of primary teeth . MATERIAL S AND METHODS Flat dentin surfaces from 50 primary molars were r and omly assigned to 10 groups according to substrate ( sound dentin [ SD ] vs caries-affected dentin [ CAD ] pH cycled for 14 days ) and bonding approach ( Scotchbond Universal Adhesive : self-etching , vs dry or wet-bonding etch- and -rinse strategies ; Adper Single Bond Plus [ two-step etch- and -rinse adhesive ] and Clearfil SE Bond [ two-step self-etching system ] as controls ) . After 24 h of water storage , bonded sticks with cross-sectional areas of 0.8 mm2 were tested for microtensile bond strength ( μTBS ) . Two sticks from each tooth were immersed in silver nitrate solution in order to evaluate nanoleakage ( NL ) with SEM . The μTBS means were analyzed using two-way ANOVA and Tukey 's tests . For NL , the Kruskal-Wallis and Mann-Whitney tests were used ( α = 0.05 ) . RESULTS The influence of the etching strategy on the bonding performance of the universal adhesive was substrate dependent . The self-etching approach result ed in lower μTBS values and higher silver nitrate uptake into hybrid layers for Scotchbond Universal Adhesive on SD , while no difference among experimental groups was observed in CAD . CONCLUSION It is preferable to use the universal adhesive following either a dry- or wet-bonding etch- and -rinse approach on both sound and caries-affected primary dentin The purpose of this study was to investigate the influence of chlorhexidine ( CHX ) concentration on the microtensile bond strength ( muTBS ) of contemporary adhesive systems . Eighty bovine central incisors were used in this study . The facial enamel surface of the crowns was abraded with 600-grit silicon carbide paper to expose flat , mid-coronal dentin surfaces . The tested material s were Scotchbond Multi purpose ( SMP ) , Single-Bond ( SB ) , Clearfil SE Bond ( CSEB ) and Clearfil Tri S Bond ( CTSB ) . All the material s were applied according to manufacturer 's instructions and followed by composite application ( Z250 ) . The teeth were r and omly divided into 16 groups : for the etch- and -rinse adhesives ( SMP and SB ) , 0.12 % or 2 % CHX was applied prior to or after the acid etching procedure . For the self-etch adhesives ( CSEB and CTSB ) 0.12 % or 2 % CHX was applied prior to the primer . Control groups for each one of the adhesive systems were also set up . The specimens were immediately su bmi tted to muTBS testing and the data were analyzed using Analysis of Variance and the Tukey post hoc test ( alpha = .01 ) . The failure patterns of the specimens were observed using scanning electron microscopy . The effects of 2 % CHX were statistically significant ( p < 0.01 ) for the self-etch adhesives but were not significant for the etch- and -rinse adhesive systems . Analysis of the data demonstrated no statistical difference between the etch- and -rinse adhesive systems . CHX-based cavity disinfectants in concentrations higher than 0.12 % should be avoided prior to the self-etch adhesive systems evaluated in this study to diminish the possibilities of reduction in bond strength PURPOSE To investigate the effect of phosphoric acid etching prior to the application of self-etching primer on the adhesion of resin composite to tooth substrates . MATERIAL S AND METHODS Bovine teeth were r and omly divided in four groups of 20 sample s each . Tooth surface conditions were as follows : Group 1 : enamel ground with 600-grit SiC paper ; Group 2 : ground enamel was etched with 35 % phosphoric acid gel for 15 seconds , water-rinsed and completely dried ; Group 3 : ground dentin ; Group 4 : dentin etched using the same method as Group 2 . The sample s in each group were divided in two subgroups of 10 each . UniFil Bond ( UB ) and Clearfil SE Bond ( SE ) were applied as adhesive systems with self-etching primers , and a layer of resin composite ( AP-X ) was placed and light-cured for 40 seconds . The tensile bond strengths ( TBS ) were measured and the resin-tooth interfaces were observed with scanning electron microscopy . RESULTS The mean TBS values were 11.2 MPa ( Group 1-UB ) , 14.3 MPa ( Group 1-SE ) , 16.3 MPa ( Group 2-UB ) , 20.5 MPa ( Group 2-SE ) , 13.4 MPa ( Group 3-UB ) , 16.7 MPa ( Group 3-SE ) , 9.3 MPa ( Group 4-UB ) and 12.6 MPa ( Group 4-SE ) . Two-way ANOVA and Scheffe 's F test showed that the enamel etching significantly increased the TBS values but dentin etching significantly decreased the TBS values PURPOSE To evaluate the bonding of simplified adhesive systems to sound and caries-affected dentin of primary teeth with microtensile ( µTBS ) and nanoleakage ( NL ) tests . MATERIAL S AND METHODS Occlusal cavities were prepared in 36 sound second primary molars . Half of the specimens were su bmi tted to pH cycling to simulate caries-affected dentin . Teeth were r and omly restored with one of three material s : the etch- and -rinse adhesive system Adper Single Bond 2 ( SB ) , the two-step self-etching adhesive system Adper SE Plus ( SE ) , and the one-step self-etching adhesive system Adper Easy One ( EASY ) . After storage for 24 h , specimens with cross-sectional areas of 0.8 mm2 were prepared for microtensile testing ( 1 mm/min ) . One stick from each tooth was immersed in silver nitrate solution ( 24 h ) and allowed to develop for 8 h in order to score the nano leakage with SEM . The fracture pattern was evaluated using a stereomicroscope ( 400X ) . The µTBS means were analyzed by two-way ANOVA and Tukey 's post-hoc test . For NL , the Kruskal- Wallis and Mann-Whitney tests were used ( α < 0.05 ) . RESULTS SB ( 35.5 ± 3.5 ) showed the highest µTBS value to sound dentin , followed by EASY ( 26.3 ± 1.9 ) and SE ( 18.2 ± 6.5 ) ( p < 0.05 ) . No difference among material s was observed for caries-affected dentin ( SB : 17.8 ± 4.2 ; SE : 13.9 ± 3.2 ; EASY : 14.4 ± 4.2 , p > 0.05 ) . For all groups , adhesive/mixed fracture prevailed . Caries affected dentin promoted silver nitrate uptake into the adhesive interface ; however , with SE , the nano leakage was more pronounced than in the other adhesive systems , even in sound dentin . CONCLUSION Caries-affected dentin negatively influences the bond strength and nano leakage of the two-step etch- and -rinse and one-step self-etching adhesive systems tested in primary teeth AIM The aim of this in vitro study is to evaluate the effects of three different caries removal techniques on the microtensile bond strength of adhesive material s to caries-affected dentin . MATERIAL S AND METHODS Thirty primary molar teeth were used . The teeth were r and omly divided into three groups according to the caries removal technique employed : conventional steel bur ( group 1 ) ; Er : YAG laser ( group 2 ) ; chemomechanical method ( group 3 ) . Each group was divided into two subgroups according to bonding agents : one-step self-etch adhesive and etch- and -rinse adhesive . The teeth were restored with composite resin . Vertical sticks were obtained and subjected to tensile stress . Data were analyzed by two-way analysis of variance ( ANOVA ) , Tukey 's test and an independent sample s t-test . RESULTS The values for the laser groups were significantly lower than those of the bur groups for both bonding agents ( p<0.05 ) . There were no significant differences between the bur and chemomechanical groups ( p > 0.05 ) . CONCLUSION Bur and chemomechanical techniques in primary teeth were found more successful . Similar results were found according to the adhesives used for each caries removal techniques The purpose of this study was to evaluate the influence of intrapulpal pressure and dentin depth on bond strengths of an etch- and -rinse and a self-etching bonding agent to dentin in vitro and in vivo . Twenty-four pairs of premolars were r and omly divided into four groups ( n = 6 ) according to the dentin bonding agent , Single Bond and Clearfil SE Bond , and intrapulpal pressure , null or positive . Each tooth of the pair was further design ated to be treated in vivo or in vitro . The intrapulpal pressure was controlled in vivo by the delivery of local anesthetics containing or not a vasoconstrictor , while in vitro , it was achieved by keeping the teeth under hydrostatic pressure . Class I cavities were prepared and the dentin bonding agents were applied followed by incremental resin restoration . For the teeth treated in vitro , the same restorative procedures were performed after a 6 month-storage period . Beams with 1 mm(2 ) cross-sectional area were prepared and microtensile tested . Clearfil SE Bond was not influenced by any of the variables of the study , while bond strengths produced in vitro were significantly higher for Single Bond . Overall , lower bond strengths were produced in deep dentin , which reached statistical significance when Single Bond was applied under physiological or simulated intrapulpal pressure . In conclusion , in vitro bonding may overestimate the immediate adhesive performance of more technique-sensitive dentin bonding systems . The impact of intrapulpal pressure on bond strength seems to be more adhesive dependent than dentin morphological characteristics related to depth ABSTRACT Objective Green tea extract has been advocated as a matrix metalloproteinase ( MMP ) inhibitor ; however , its effect on bond durability to caries-affected dentin has never been reported . Thus , the aim of this in vitro study was to evaluate the effect of two MMP inhibitors ( 2 % chlorhexidine and 2 % green tea extract ) , applied after acid etching , on bond durability of an etch- and -rinse adhesive system to caries-affected dentin . Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface , and the molars were su bmi tted to a caries induction protocol for 15 days . After removal of infected dentin , specimens were conditioned with 37 % phosphoric acid ( 15 seconds ) and r and omly divided into three groups , according to the type of dentin pretreatment ( n=10 ) : NT : no treatment ; GT : 2 % green tea extract ; CLX : 2 % chlorhexidine . The etch- and -rinse adhesive system ( Adper ™ Single Bond 2 , 3 M ESPE , St. Paul , MN , USA ) was applied according to the manufacturer 's instructions , and composite resin restorations were built on the dentin . After 24 hours , at 37 ° C , the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks ( 0.8 mm2 of adhesive area ) and r and omly subdivided into two groups according to when they were to be su bmi tted to microtensile bond strength ( μTBS ) testing : immediately or 6 months after storage in distilled water . Data were reported in MPa and su bmi tted to two-way ANOVA for completely r and omized blocks , followed by Tukey ’s test ( α=0.05 ) . Results After 24 hours , there was no significant difference in the μTBS of the groups . After 6 months , the GT group had significantly higher μTBS values . Conclusion It was concluded that the application of 2 % green tea extract was able to increase bond durability of the etch- and -rinse system to dentin . Neither the application of chlorhexidine nor non-treatment ( NT - control ) had any effect on bond strength after water storage PURPOSE The objective of this study was to assess in vitro the tensile bond strength of a self-etching and 2 total-etch , single-bottle adhesive systems to primary dentin . METHODS Thirty-six sound primary canine buccal surfaces were r and omly assigned to 3 groups ( N = 12 ) , corresponding to the tested adhesive systems : ( 1 ) group I = Excite ( EX ) ; ( 2 ) group II = Single Bond ( SB ) ; and ( 3 ) group III = Prompt L-Pop ( PLP ) . After 24-hour storage in distilled water , tensile bond strength was tested for failure at a crosshead speed of 0.5 mm/min . RESULTS Means ( MPa ) and st and ard deviation ( + /- ) were : ( 1 ) EX = 12.72 ( + /- .89 ) ; ( 2 ) SB = 10.86 ( + /- 2.09 ) ; ( 3 ) PLP = 8.66 ( + /- 2.23 ) . Single Bond and Excite showed statistically similar results ( P > .05 ) and provided the highest means . Prompt L-Pop was statistically different from the other groups ( P < .05 ) and reached the lower bond strength to primary dentin . CONCLUSIONS Total-etch agents provided the best overall bonding performance , whereas the all-in-one , self-etching , self-priming adhesive system yielded remarkably lower bond strength to primary teeth dentin PURPOSE To evaluate the effect of chemical and microbiological methods of caries induction on microtensile bond strength ( μTBS ) of current adhesive systems to primary dentin . MATERIAL S AND METHODS Flat dentin surfaces from 36 primary molars were assigned to 3 groups according to the method of inducing caries-affected dentin : ( 1 )  control ( sound dentin ) ; ( 2 )  pH cycling ; and ( 3 ) microbiological . In both methods , teeth were su bmi tted to caries induction for 14 days , and the sound dentin was stored in distilled water for the same period . Specimens were then r and omly reassigned according to adhesive system : a two-step etch- and -rinse adhesive ( Adper Single Bond 2 ) or a two-step self-etching system ( Clearfil SE Bond ) . Composite buildups were constructed and the teeth were sectioned to obtain bonded sticks ( 0.8 mm2 ) to be tested for microtensile bond strength . The μTBS means were analyzed by two-way ANOVA and Tukey 's tests ( α = 0.05 ) . Failure mode was evaluated using a stereomicroscope ( 400X ) . RESULTS Both methods of caries induction result ed in lower μTBS values ( with no significant difference between them ) than those obtained for sound dentin . Adhesive systems showed similar bond strength values . The percentage of premature failure was higher in the microbiological group , regardless of adhesive system . CONCLUSION Microbiological and pH-cycling methods are both suitable for simulating caries-affected dentin for bonding evaluations in primary teeth OBJECTIVES The aim was to determine the effect of blood contamination and haemostatic agent application on the shear bond strength ( SBS ) of different adhesives to dentin . MATERIAL S AND METHODS Seventy-two extracted human molars were used in this study . Teeth were mounted acrylic in moulds . Mesial and distal surfaces were removed to obtain flat dentin surfaces ( n=144 ) and grinded with a 600 grit s and paper . The sample s were r and omly divided into three main groups ( N=48 ) based on the adhesive system and application procedures . GROUP 1 : No contamination ( control ) , GROUP 2 : Blood contamination , GROUP 3 : Blood Contamination+Haemostatic agent application . Each group was further divided into four subgroups : SUBGROUP I : Single Bond 2 ( etch- and -rinse ) Subgroup II : Clearfil SE Bond ( two-step self-etch ) Subgroup III : Single Bond Universal ( multimode , etch- and -rinse ) subgroup IV : Single Bond Universal ( multimode , all-in-one self-etch ) ( n=12 ) . Resin cylinders ( Filtek Z550 ) were bonded to the dentin surfaces according to the manufacturers ' instructions . A shear load was applied to the specimens using universal testing machine at a cross-head speed of 0.5 mm/min until failure . Data were analyzed statistically ( p<0.05 ) . RESULTS Statistically significant differences were observed between no contamination ( control ) and blood + haemostatic agent contamination groups for both Single Bond 2 ( etch- and -rinse ) and Single Bond Universal ( multimode , all-in-one self-etch ) ( p<0.05 ) . When the adhesive systems were compared to each other , no statistically significant differences were found in all contamination groups and control group for the mean SBS values ( p>0.05 ) . CONCLUSION When blood contamination is inevitable two step self- etching adhesive systems may be the choice of adhesive system in terms of shear bond strength The aim of this in vitro study was to investigate the effect of the cariostatic and preventive agent silver diamine fluoride ( SDF ) on the microtensile bond strength of resin composite to dentin . Forty-two caries-free , extracted molars were flattened occlusally and apically using a diamond saw , and the exposed occlusal dentin was polished with a series of silicon carbide papers , all under water irrigation . The teeth were then r and omly divided into six groups of seven teeth each that were treated as follows : 1 ) Peak SE self-etch bonding agent ; 2 ) 12 % SDF + Peak SE ; 3 ) 38 % SDF + Peak SE ; 4 ) Peak LC etch- and -rinse bonding agent ; 5 ) 12 % SDF + Peak LC ; and 6 ) 38 % SDF + Peak LC . Four-millimeter buildups of Amelogen Plus were incrementally placed on all teeth ; after a 24-hour storage period in distilled water , the specimens were sectioned perpendicular to the adhesive interface to produce beams of cross-sectional surface area measuring approximately 1 mm(2 ) . The beams were placed on a microtensile testing machine , which utilized a single-speed pump motor and force gauge at 20 kgf × 0.01 second to record maximum tensile force before failure occurred . Two-way analysis of variance and post hoc Tukey tests were performed to compare the effects of the SDF  on microtensile bond strength , with statistical significance set at α = 0.05 . None of the experimental groups treated with different concentrations of SDF showed a significant difference in bond strength compared to the control groups , and there was no significant difference in bond strength between self-etch and etch- and -rinse groups . However , the effect of SDF  on self-etch bonded teeth compared to etch- and -rinse bonded teeth was statistically significant ( p=0.0363 ) , specifically at the 12 % concentration . SDF does not adversely affect the bond strength of resin composite to noncarious dentin The bonding performance of current adhesive systems to primary enamel has not been thoroughly research ed . This study compared the micro-shear bond strength of two adhesive systems to primary and permanent tooth enamel . Two commercially available resin adhesives , a self-etching primer system ( Clearfil SE Bond ) and a single-bottle adhesive system ( Single Bond ) used with a total-etch wet bonding technique were tested . A micro-shear bond test was used to examine the adhesive systems on mid-coronal buccal enamel of extracted primary or permanent teeth . In addition , etched enamel surfaces and etched-bonded enamel interfaces were examined using scanning electron microscopy ( SEM ) . No statistically significant differences of shear bond strength values were found between the primary and permanent enamel or the adhesive systems used ( p>0.01 ) . The SEM observations showed that both adhesive systems etched the primary enamel deeper than the permanent enamel , suggesting that the action of acid etch seemed to be more intense on primary enamel than on permanent enamel . Bonding of the adhesive systems to primary enamel was almost identical to permanent enamel Background To assess the immediate bond strength of a dual-cure adhesive resin cement to the hybridized dentin with different bonding systems . Material and Methods Fifty-six healthy human molars were r and omly divided into 7 groups ( n=8 ) . After 3 longitudinal sections , the central cuts were included in PVC matrix and were su bmi tted to dentin hybridization according to the groups : G1 - etch & rinse system with 3-step ( Apder ™ Scotchbond ™ Multi- Purpose , 3 M ESPE ) , G2 - etch & rinse system with 3-step ( Optibond ™ FL , Kerr ) , G3 - etch & rinse system with 3-step ( All-Bond 3 ® , Bisco ) , G4 - etch & rinse simplified system ( Adper ™ Single Bond 2 , 3 M ESPE ) , G5 - self-etching system with one step ( Bond Force , Tokuyama ) , G6 - universal system in moist dentin ( Single Bond Universal , 3 M ESPE ) , G7 - universal system in dry dentin ( Single Bond Universal , 3 M ESPE ) . Then all groups received the cementing of a self-adhesive resin cement cylinder ( Duo-link , Bisco ) made from a polypropylene matrix . In the evaluation of bond strength , the sample s were subjected to the microshear test and evaluated according to the fracture pattern by optical microscopy . Results The Kruskal-Wallis test suggests a statistically significant difference between groups ( p=0,039 ) , and Tukey for multiple comparisons , indicating a statistically significant difference between G3 and G4 ( p<0.05 ) . It was verified high prevalence of adhesive failures , followed by mixed failure and cohesive in dentin . Conclusions The technique and the system used to dentin hybridization are able to affect the immediate bond strength of resin cement dual adhesive . Key words : Adhesion , adhesive resin cement , adhesive systems , microshear OBJECTIVES To evaluate the effect of shortening the etching time on roughness , microhardness and bond strength of three adhesive systems to primary tooth dentin . METHODS Flat dentin surfaces from primary molars were r and omly assigned to six experimental groups . Three different adhesive systems were used : an etch- and -rinse adhesive ( Single Bond ) , a two-step self-etching ( Clearfil SE Bond ) , and a one-step self-etching ( One-Up Bond F ) adhesive . In half of the specimens , the recommended etching time was used , in the other half the etching time was 50 % reduced . After applying the adhesive , resin composite build-ups were constructed and stored in a humid environment for 24h at 37 degrees C. Specimens were sectioned into 1mm(2 ) beams and tested for microtensile bond strength ( MTBS ) . Debonded surfaces were analyzed by scanning electron microscopy ( SEM ) . Additional surfaces were conditioned for microhardness measurements ( KHN ) and for atomic force microscopy ( AFM ) analysis . Intertubular and total surface roughness ( Ra ) were recorded . Results were analyzed with ANOVA and Student-Newman-Keuls tests ( P<0.05 ) . RESULTS Single Bond and Clearfil SE Bond showed higher MTBS than One-Up Bond F. Bond strength and intertubular roughness increased when Single Bond and One-Up Bond F were used with a reduced etching time . For Clearfil SE Bond no differences in MTBS were detected when reducing the etching time . The application of phosphoric acid , Clearfil SE Bond primer and One-Up Bond F decreases dentin microhardness . CONCLUSIONS Shortening One-Up Bond F application time and reducing the etching time of phosphoric acid to one-half of the manufacturer 's recommended etching time when using Single Bond are recommended when bonding to primary dentin Aim : This study investigated the effect of one hemostatic agent on the shear bond strength of  self-etch and etch- and -rinse adhesive systems . Material s and Methods : Sixty extracted third molars were selected . After preparing a flat surface of superficial dentin , they were r and omly divided into six groups . Adhesives were Tetric N-Bond , AdheSE , and AdheSE One F. Before applying adhesives , surfaces were contaminated with ViscoStat for 60 s in three groups and rinsed . Then composite were attached to surfaces and light cured . After thermocycling , the bond strength was calculated and failure modes were determined by stereomicroscope . The data were analyzed by t-test and one-way ANOVA with P < 0.05 as the level of significance . Results : ViscoStat had significantly decreased the shear bond strength of AdheSE ( P < 0.0001 ) to dentin . Modes of failures in all groups were mainly adhesive . Conclusion : Contamination had an adverse effect on the shear bond strength of AdheSE and reduced it This study examined the effect of phosphoric acid ( PA ) etching on the bond strength and acid-base resistant zone ( ABRZ ) formation of a two-step self-etching adhesive ( SEA ) system to enamel . An etch- and -rinse adhesive ( EAR ) system Single Bond ( SB ) and a two-step SEA system Clearfil SE Bond ( SE ) were used . Human teeth were r and omly divided into four groups according to different adhesive treatments : 1 ) SB ; 2 ) SE ; 3 ) 35 % PA etching→SE primer→SE adhesive ( PA/SEp+a ) ; ( 4 ) 35 % PA etching→SE adhesive ( PA/SEa )  . Microshear bond strength to enamel was measured and then statistically analyzed using one-way analysis of variance and the Tukey honestly significant difference test . The failure mode was recorded and analyzed by χ ( 2 ) test . The etching pattern of the enamel surface was observed with scanning electron microscope ( SEM ) . The bonded interface was exposed to a demineralizing solution ( pH=4.5 ) for 4.5 hours and then 5 % sodium hypochlorite with ultrasonication for 30 minutes . After argon-ion etching , the interfacial ultrastructure was observed using SEM . The microshear bond strength to enamel of the SE group was significantly lower ( p<0.05 ) than that of the three PA-etched groups , although the latter three were not significantly different from one another . The ABRZ was detected in all the groups . In morphological observation , the ABRZ in the three PA-etched groups were obviously thicker compared with the SE group with an irregular wave-shaped edge BACKGROUND The objec tives of this study were to determine microtensile bond strengths of two dentin adhesives and to compare the micromorphological structure of the resin/dentin interface in caries-affected dentin with that of intact dentin . METHODS The authors r and omly divided 40 proximal dentinal carious primary teeth and 40 noncarious anterior primary teeth into two groups ( self-etching and total-etching ) . They used a caries-detecting dye as an indicator of the need to remove the outer carious dentin . The authors restored the teeth with a hybrid resin-based composite . After 24 hours ' storage in 37 C water , specimens were sectioned and shaped to form a curved section with a cross-sectional area of 1 square millimeter , then tension was applied until they fractured . The authors prepared the resin/dentin interfaces for the two bonding systems and examined them in 10 occlusal carious and 10 noncarious teeth . STATISTICAL ANALYSIS The bond strengths for intact and caries-affected dentin within the same group were analyzed via a t test . The authors compared the remaining dentin thickness ( RDT ) and dentin hardness using analysis of variance and the least significant difference test at the .05 level of significance . RESULTS The self-etching adhesive demonstrated no statistical difference in bond strength between intact and caries-affected dentin . However , the total-etching adhesive demonstrated different bond strengths for intact and caries-affected dentin . Moreover , the RDT of specimens with intact and caries-affected dentin was not significantly different , whereas the dentin hardness of caries-affected dentin was significantly lower than that of intact dentin . The authors found a thicker hybrid layer in intact and caries-affected dentin of specimens in the total-etching group . CONCLUSION The adhesives exhibited significantly different bond strengths in intact dentin of primary teeth . However , they exhibited similar bond strengths in caries-affected dentin OBJECTIVES The aim of this study was to evaluate the clinical long-term retention to dentin of seven adhesive systems . METHODS A total of 270 Class V restorations of four etch- and -rinse , one self-etch adhesive system and a resin-modified glass ionomer cement were placed in non-carious cervical lesions without intentional enamel involvement . The restorations were evaluated at baseline , 6 , 12 , 18 , and 24 months and then every year during a 13-year follow-up . Dentin bonding efficiency was determined by the percentage of lost restorations . RESULTS During the 13 years , 215 restorations could be evaluated . The cumulative loss rate at 13 years was 53.0 % , with significant different failures rates for the different systems varying between 35.6 and 86.8 % . Four systems fulfilled the ADA 18-month full acceptance retention criteria . Two systems showed at 18 months and earlier high debonding rates . The annual failure rates for the etch- and -rinse systems were Optibond 3.1 % , Permagen 13.0 % , Scotchbond MP 4.8 % , Syntac classic 2.8 % ; for the self-etch system P&S 4.4 % ; and the resin-modified glass ionomer cement Vitremer 2.7 % . CONCLUSION It can be concluded that all systems showed a continuous degradation of the bond with a wide variation , which was independent of the adhesion strategy . Three bonding systems showed a cumulative failure rate after 13 years between 36 and 41 % with the best retention for the resin-modified glass ionomer cement and a four-step etch- and -rinse system OBJECTIVES The aim of this study was to evaluate the bonding of four commercially available adhesive systems to ground and intact enamel surfaces . METHODS Extracted human teeth were used to measure the microtensile bond strength to enamel and a field-emission scanning electron microscopy ( FE-SEM ) was used to observe the bonded interface and the effect of the surface conditioning of each material . Intact buccal enamel surfaces were cleansed with tooth paste using a rotary dental brush , and the ground enamel surfaces were prepared by reducing approximately 0.5 mm from the buccal enamel surfaces using a high-speed diamond bur . One-Step ( OS , Bisco ) , Single Bond ( SB , 3 M ) , Clearfil Liner Bond II ( LBII , Kuraray ) , and Tokuso Mac Bond II ( MBII , Tokuso ) were evaluated for their ability to bond to enamel . RESULTS There was no significant difference in bond strengths between the material s when they were applied to ground enamel surfaces ( p > 0.05 ) . However , the bond strengths of the self-etching systems , LBII and MBII , had significantly lower bond strengths to intact enamel than the bonding systems OS and SB using phosphoric acid etching ( p < 0.05 ) . FE-SEM revealed that the etching pattern of self-etching primers was not deep enough to obtain good penetration of bonding resin when applied to intact enamel surfaces . CONCLUSIONS Phosphoric acid etching produced good resin adhesion to ground and intact enamel . The self-etching/self-priming systems also produced good adhesion to ground enamel , but had lower bond strengths to intact enamel OBJECTIVES This study evaluated the bond strength of luting systems for bonding glass fiber posts to root canal dentin . The hypothesis tested was that there are no differences in bond strength of glass fiber posts luted with different cement systems . METHODS Forty bovine incisors were r and omly assigned to five different resin cement groups ( n=8 ) . After endodontic treatment and crown removal , translucent glass fiber posts were bonded into the root canal using five different luting protocol s ( self-cured cement and etch- and -rinse adhesive system ; dual-cured cement and etch- and -rinse adhesive system ; self-cured cement and self-etch adhesive system ; dual-cured cement and self-etch adhesive system ; and dual-cured self-adhesive cement ) . Push-out bond strength was evaluated at three different radicular levels : cervical , middle , and apical . The interface between resinous cement and the post was observed using a stereoscopic microscope . RESULTS Analysis of variance showed a statistically significant difference among the cements ( p<0.05 ) and the root canal thirds ( p<0.05 ) . The self-adhesive resinous cement had lower values of retention . CONCLUSIONS The resin cements used with etch- and -rinse and self-etch adhesive systems seem to be adequate for glass fiber post cementation BACKGROUND Self-etching adhesives are believed to prevent postoperative sensitivity when used under posterior resin-based composite restorations . The authors tested a twofold hypothesis : a self-etch , or SE , adhesive would result in less postoperative sensitivity than a total-etch , or TE , adhesive ; an SE adhesive would result in poorer enamel marginal integrity than a TE adhesive . METHODS Patients were selected on the basis of requiring Class I and II restorations in molars and premolars . The authors placed 30 restorations with the SE material ( Clearfil SE Bond , Kuraray America , New York ) and 36 restorations with Prime & Bond NT ( Dentsply Caulk , Milford , Del. ) , which uses 34 percent phosphoric acid to etch enamel and dentin simultaneously . Preparations were of st and ard design , with all margins in enamel without beveling . Upon rubber dam isolation , the authors conditioned the enamel and dentin walls with the self-etching primer ( for Clearfil SE Bond ) or etched with the proprietary 34 percent phosphoric acid ( for Prime & Bond NT ) , followed by application of the corresponding dentin adhesive . Teeth were restored with the proprietary hybrid resin-based composite indicated for posterior restorations : Clearfil AP-X for Clearfil SE Bond or Esthet-X Micro Matrix Restorative for Prime & Bond NT . The restored teeth were evaluated preoperatively and at two weeks , eight weeks and six months postoperatively for sensitivity to cold ( ice ) , air and masticatory forces , as well as for marginal discoloration . RESULTS Analysis of variance revealed no statistically significant differences in postoperative sensitivity between the SE and TE material s at any recall time . Marginal discoloration was rated as " absent " for all restorations at six months . Only one tooth displayed sensitivity to occlusal forces at six months . CONCLUSION The SE adhesive did not differ from the TE adhesive in regard to sensitivity and marginal discoloration . CLINICAL IMPLICATION S Postoperative sensitivity may depend on the restorative technique rather than on the type of dentin adhesive used UNLABELLED The self-etch approach provides dentists with a generation of user-friendly and less technique-sensitive adhesives . Nevertheless , some concern has been raised regarding their bonding effectiveness to enamel , in particular when so-called ' mild ' self-etch adhesives are employed . OBJECTIVES The purpose of this study was to test the hypothesis that the two-step self-etch adhesive Clearfil SE Bond ( C-SE ; Kuraray , Osaka , Japan ) bonds equally effective to enamel/dentin either with or without prior etching with phosphoric acid . METHODS Bur-cut enamel/dentin surfaces prepared from human molars were partially split in two halves by cutting a shallow groove . One half was first etched with 40 % phosphoric acid ( K-etchant ) , while protecting the other half by holding a razor blade in the groove . Next , C-SE was applied strictly following the manufacturer 's instructions , after which the surface was built up using Z100 ( 3 M Espe ) . After 24-h water storage , micro-specimens were prepared with the interface circularly constricted using a Micro-Specimen Former , prior to micro-tensile bond strength ( MPa ) measurement . In addition , interfaces of C-SE with enamel/dentin prepared with and without beforeh and acid etching were examined by Feg-SEM and TEM . RESULTS Beforeh and etching significantly increased the bonding effectiveness of C-SE to enamel . A clearly more micro-retentive surface was revealed by TEM and Feg-SEM when enamel was etched . Phosphoric-acid etching prior to C-SE application on dentin significantly decreased the muTBS to dentin . TEM provided indications of a low- quality hybrid layer after beforeh and phosphoric-acid etching . CONCLUSION Using C-SE , additional etching with phosphoric acid to improve bonding effectiveness should be limited to enamel
RESULTS No statistical significant difference in bond strength between both categories was observed in caries-affected dentin at immediate evaluation and in sound dentin after ageing .
Etch- and -rinse adhesives , however , performed better in sound enamel and dentin substrates considering immediate bond strength .
None study assessed the long-term adhesive effectiveness to sound or demineralized enamel .
Although the articles included in this meta- analysis showed high heterogeneity and high risk of bias , the in vitro literature suggests superior performance of etch- and -rinse adhesives in primary teeth in comparison with self-etch systems
AIM To systematic ally review the literature for in vitro studies that evaluated the immediate or after ageing bond strength of  etch- and -rinse and self-etch adhesive systems to enamel and dentin of primary teeth .