Polyphosphate Maintains Intestinal Homeostasis Figure 6. Poly P prevented F-actin and E-cadherin degradation induced by the oxidative stress. (A–F) Caco2/BBE cells were treated with 10 mg/mL of poly P, 10 mM of Gly-Arg-Gly-Asp-Ser (GRGDS) or Ser-Asp-Gly-Arg-Gly (SDGRG) overnight, followed by stimulated with 30 mM of H2O2 for 1 h. (G, H) Caco2/BBE cells were treated with 10 mg/mL of poly P overnight, followed by stimulated with 0.3 mM of NH2Cl for 1 h. F-actin was stained with phalloidin (green) and E-cadherin (red). Nuclei were counterstained with 49,6-Diamidino-2-Phenylindole (DAPI) (blue). doi:10.1371/journal.pone.0023278.g006 PLoS ONE | www.plosone.org 10 August 2011 | Volume 6 | Issue 8 | e23278