highest break number (red). The timing distribution for the whole genome is shown as a white box. (B) The meiS replication time of DSB HSs mapped by ssDNA enrichment (left panel) or Spo11 genome-wide location analysis (center panel), or axis association sites (right panel) are plotted as a function of their time of replication in mitS. Red line indicates the predicted trend if relative replication time were identical in meiS and mitS. (TIF) Figure S7 Axis sites. Genome-wide localization analysis was performed for Rec8 (shown in purple), in wild-type cells with and without HU (H4471) cdc6-mn cells (H5491) and clb5D clb6D cells (H6495). Hop1 localization analysis is shown in green for wild-type cells without HU (H119, [67]), with HU (H4471), cdc6-mn cells (H154) and clb5D clb6D cells (H2017). Red1 localization analysis is shown in red for wild-type cells without HU (H119), with HU (H4471), cdc6-mn cells (H154) and clb5D clb6D cells (H2017). The enrichment of immunoprecipitated over input DNA is plotted for chromosome III. Sites that showed significant coincident binding for Rec8, Hop1 and Red1 are indicated by inverted black triangles above the plots. Black dots indicate the positions of the centromere. (TIF) Figure S8 ssDNA enrichment profiles. The ssDNA enrichment profiles for dmc1D cells (blue, [67]), and dmc1D cdc6-mn cells (H1584, orange, enrichment downwards) were plotted with respect to position for all 16 yeast chromosomes. Black dots indicate the positions of centromeres. (TIF) References 1. Moens PB, Pearlman RE (1988) Chromatin organization at meiosis. Bioessays 9: 151–153. 2. Zickler D, Kleckner N (1999) Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33: 603–754. 3. Williamson DH, Johnston LH, Fennell DJ, Simchen G (1983) The timing of the S phase and other nuclear events in yeast meiosis. Exp Cell Res 145: 209–217. 4. 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