APT-2-Mediated Deacylation of Peripheral Proteins Figure 2. Deacylation kinetic of single acylated N13GAP-43(C3S). A) 60 h after coexpressing GAP-43full-YFP (GAP-43full), N13GAP-43-YFP (N13GAP-43), their S-acylation mutants (C3S, C4S or C3,4S) and N27GalNAc-T-CFP (GalNAc-T), CHO-K1 cells were incubated with Alexa Fluor 647-Tf (Tf) to label the RE and analyzed by live cell confocal fluorescent microscopy. The fluorescence signals from YFP, CFP and Alexa Fluor 647 were pseudocolored green, blue and red, respectively. Each panel shows merged images from the three acquired channels. The insets in each panel show PLoS ONE | www.plosone.org 4 November 2010 | Volume 5 | Issue 11 | e15045