Human Cytomegalovirus UL114 Interact with SMARCB1 Figure 2. SMARCB1 and UL114 interact in vitro. (A) In vitro binding analysis of HA-tagged D379-SMARCB1 and c-myc-tagged UL114 in 35S- labeled proteins using the TNT coupled transcription/translation system. The proteins were transcribed and translated in vitro with 35S-methionine in the translation mixture to generate radioactive labeled products from vectors pACT2-D379-SMARCB1 (HA-epitope) and pGBKT7-UL114 (c-myc epitope). The translated D379-SMARCB1-HA and UL114-c-myc were immunoprecipitated with either anti-HA or anti-c-myc-antibodies and eluted from the Protein G beads. Samples were subjected to 8% SDS-PAGE and PhosphoImaging. Lane 1: UL114-c-myc+c-myc antibody. Lane 2: D379- SMARCB1-HA+HA-antibody. Lane 3: D379-SMARCB1-HA+UL114-c-myc+HA-antibody. Lane 4: D379-SMARCB1-HA+UL114-c-myc+c-myc antibody. PLoS ONE | www.plosone.org 4 March 2012 | Volume 7 | Issue 3 | e34119