APT-2-Mediated Deacylation of Peripheral Proteins Figure 1. GAP-43 deacylation in CHO-K1 cells. A) 24 h after transfection with plasmids for GAP-43full-YFP or N13GAP-43-YFP, CHO-K1 cells were incubated with 50 mM 2-BP (2-BP) or vehicle (Control) in the presence (+Inh) or absence (-Inh) of protein degradation inhibitors for 0, 3 and 6 h. At each time point, cells were lysed and homogenates Western blotted with an antibody to GFP. The lower panels show Western blot with antibody to a-tubulin. B) 60 h after transfection, CHO-K1 cells expressing GAP-43full-YFP or N13GAP-43-YFP were treated with 50 mM 2-BP (2-BP) or vehicle (Control) for 0, 3 and 6 h in presence of CHX and protein degradation inhibitors. At each time point the subcellular distribution was analyzed by live cell confocal fluorescent microscopy. Scale bars: 5 mm. doi:10.1371/journal.pone.0015045.g001 PLoS ONE | www.plosone.org 3 November 2010 | Volume 5 | Issue 11 | e15045