Redundancy in Plant Defense Figure 4. Interaction phenotypes of virulent or AvrRPT2-expressing P. syringae with eds1 sid2 plants. (A) Photograph showing phenotypes produced upon infiltration of 105 CFU/ml bacteria (AvrRPT2). All genotypes were in the Col-0 background. The leaves were photographed at 3 days post inoculation (dpi). The pathogen-inoculated EDS1 SID2 F2 plants showed absence of any visible symptoms in response to bacterial inoculations, similar to Col-0 plants (data not shown). (B) Growth of virulent or avirulent (expressing AvrRPT2) P. syrinage on indicated genotypes. The error bars indicate SD. Asterisks and omega symbols indicate data statistically significant from wt (Col-0) or sid2 (P,0.05, n = 4), respectively. All genotypes are in the Col-0 background. (C) Levels of HA-tagged RPS2 protein at 0, 2, 4, 8, and 24 h post inoculation with P. syringae expressing AvrRPT2. Levels of Rubisco were used as the loading control. (D) Levels of HA-tagged RPS2 protein in indicated genotypes. Levels of Rubisco were used as the loading control. doi:10.1371/journal.pgen.1000545.g004 PLoS Genetics | www.plosgenetics.org 8 July 2009 | Volume 5 | Issue 7 | e1000545