GSK-3 Inhibition Ameliorates Ab Pathology Figure 9. Flies expressing Ab42 in adult neurons show no changes in total tau phosphorylation and at Ser 262 and Ser 356 specific epitopes. (A) Western blot analyses for total tau phosphorylation using phos-tag gels and (B) phospho Ser262 and Ser356. Tau showed no changes in phosphorylation in flies expressing Ab42 (UAS-ArcAb42/GFP;elavGS/+) in comparison to their 2RU controls, and when the Ab42 expressing flies were fed lithium (+RU +Li). Flies were collected 17 days post RU induction, and maintained at 27uC. Quantification of the western blot analysis in (A), phospho- tau or non-phospho-tau normalised to total tau per sample (n = 4), and in (B) n = 4 for total tau and n = 3 for the Ser sites depicted in the bar chart, showed no significant differences between ArcAb42/+;elavGS/+ +RU flies to 2RU controls or to ArcAb42/+;elavGS/+ +RU +Li flies (one-way ANOVA). doi:10.1371/journal.pgen.1001087.g009 PLoS Genetics | www.plosgenetics.org 11 September 2010 | Volume 6 | Issue 9 | e1001087