PEITC Targets EGFR to Suppress Ovarian Cancer Figure 5. PEITC inhibits AKT in ovarian cancer cells. Representative blots of the concentration dependent effect of PEITC on p-AKT (Ser-473) and AKT in (A) SKOV-3, (B) OVCAR-3 and (C) TOV-21G ovarian cancer cells. Actin was used as loading control. *p,0.05 when compared to control. (D) Concentration dependent effect of PEITC for 24 h on AKT kinase activity as represented by phosphorylation of GSK at Ser-21/9 in OVCAR-3 or TOV- 21G cells. doi:10.1371/journal.pone.0043577.g005 PLOS ONE | www.plosone.org 7 August 2012 | Volume 7 | Issue 8 | e43577