Androgen Regulation of 5a-Reductase Isoenzymes Figure 2. DHT regulates the mRNA level of 5a-reductase differently in different prostate cell lines. A, LNCaP cells were treated with ethanol (vehicle only) or different concentrations of DHT (1 nM, 10 nM, 100 nM) for 24 and 48 hours. PWR-1E (B), BPH-1-AR (C), LAPC-4 (D), and C4- 2B4 (E) cells were treated the same way but for only for 24 hours. The mRNA levels of SRD5A1, SRD5A2, and SRD5A3 for all cell lines were quantified by qRT-PCR. *p,0.05, **p,0.01, ***p,0.001; 2-sided t test. doi:10.1371/journal.pone.0028840.g002 PLoS ONE | www.plosone.org 5 December 2011 | Volume 6 | Issue 12 | e28840