209 bp product. The density of each band in the 2% agarose gel was quantified using Bio-RAD Gel Quantification software. Histology of the ischemia treated cardiac tissue samples The biopsies were performed in the laboratory condition following the institutional and national ethical guideline. Mini- mum four endomyocardial samples were collected from different regions of the left side of the interventricular septum. Samples were then treated under ischemia by incubating it on ischemic vascular bed. For RL based recovery experiment, the heart tissues were pre-treated with RL for 15 min followed by incubation on ischemic vascular bed treated with RL during the period of incubation. After treatment, samples were fixed with 10% formaldehyde. Next, the samples of the non-ischemic and ischemic treated group were embedded in paraffin wax, sectioned into 5 mm slices and stained with the hematoxin-eosin stain. Next, the slides were observed under the 4X objective of a stereo microscope and imaged with Nikon Cool Pix camera adapted with the microscope. The technician responsible for examining the slides had no information about the sample types thus avoiding the biasness. Studying CK-MB activity of the ischemia treated heart tissue samples Samples isolated using the above protocol was treated under ischemia. Next, the cardiac tissues were homogenized with 1X HBS (0.4 gm NaCl, 0.0135 gm Na2HPO4. 2H2O, 0.6 gm HEPES for 100 ml, pH-7.2) containing leupeptin (1 mM), aprotinin References 1. Aoki N, Bitterman H, Brezinski ME, Lefer AM (1988) Cardioprotective actions of human superoxide dismutase in two reperfusion models of myocardial ischaemia in the rat. Br J Pharmacol 95: 735–740. 2. Marshall RC, Powers-Risius P, Reutter BW, Schustz AM, Kuo C, et al. (2003) Flow heterogeneity following global no-flow ischemia in isolated rabbit heart. Am J Physiol Heart Circ Physiol 284: 654–667. 3. 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