PI3K & ephrinBs on Spinal Nociceptive Information Figure 6. Intrathecal (i.t.) pre- or posttreatment with EphB1-Fc inhibited chronic constrictive injury (CCI)-induced neuropathic pain and spinal Fos protein expression, accompanied by the decreased expression of spinal PI3K-p110c and phosphorylated AKT (p- AKT). Consecutive intrathecal injection of EphB1-Fc on 1-h before CCI and 1-d and 2-d after CCI in the pre-treatment experiment and Intrathecal injection of a single dose of EphB1-Fc on 5-d after CCI in post-treatment experiment were performed. Paw withdrawal latency to the radiant heat and paw withdrawal threshold to mechanical stimuli were recorded on 1-d, 3-d, 5-d, 7-d after CCI in pre-treatment experiment and on 1-d, 3-d, 0.5-h,1- h,2-h,4-h,8-h after EphB1-Fc injection on 5-d,6-d and 7-d after CCI in post-treatment experiment. The expression of spinal Fos, PI3K-p110c and p-AKT was assayed on 5-d after CCI in pre-treatment experiment and at 2-h after EphB1-Fc injection on 5-d after CCI in post-treatment experiment.(A) PLoS ONE | www.plosone.org 8 August 2012 | Volume 7 | Issue 8 | e40930