Effect of IL-15 on APAP Hepatitis Figure 1. Deficiency of IL-15 exacerbates APAP-induced fulminant hepatitis in mice. (A) Survival of Il152/2 (n = 8) and WT (n = 10) mice for 5 days after APAP injection. P,0.01 for Il152/2 mice compared to WT controls. Serum levels of (B) ALT and (C) AST at indicated times after treatment with APAP. (D) Serum ALT levels and (E) liver histopathological changes at 8 hr after treatment with 200 mg/kg of APAP (H&E staining; magnification, upper panels, 64; lower panel, 610). *P,0.05; **P,0.001. Data are mean 6 SEM from 5,8 mice per group. doi:10.1371/journal.pone.0044880.g001 Figure 2. IL-15 was elevated in serum and up-regulated in KC-enriched fraction but not in total liver during AILI in WT mice. (A) IL-15 levels in mouse serum at indicated time points after APAP injection were evaluated by ELISA. Relative mRNA levels of IL-15 in (B) total liver at 0, 2 and 8 hr, and (C) KC-enriched fraction at 0 and 8 hr after APAP injection were analyzed by quantitative PCR. *P,0.05; **P,0.01. Data are mean 6 SEM from 6,8 mice per group. doi:10.1371/journal.pone.0044880.g002 PLOS ONE | www.plosone.org 4 September 2012 | Volume 7 | Issue 9 | e44880