Effect of IL-15 on APAP Hepatitis Figure 10. Effects of neutrophil and Kupffer cell elimination on AILI in mice. (A) 1.5 mg/kg vinblastine (Vin) was intravenously injected into mice 5 days before APAP challenge of mice. (B) Mice were intravenously injected with 30 mg/kg GdCl3 36,40 hr before APAP challenge. Serum ALT levels were analyzed at 4, 6 and 8 hr in Il152/2 mice, and 8 hr in WT mice after APAP injection. (C) The serum IL-15 levels, and (D) the dot plot of serum ALTs and relative IL-15 levels at 8 hr after APAP injection in WT mice with or without GdCl3 pretreatment. The ratio of ALT/IL-15 level was on the right panel. (E) Hepatic mRNA levels of TNFa and IL-1b and (F) hepatic nitrotyrosine formation (magnification 620) at 8 hr post-APAP in WT and Il152/2 mice with GdCl3 pretreatment or not. *P,0.05; **P,0.01; ***P,0.001; n.s., not significant. Data are mean 6 SEM from 5,8 mice per group. doi:10.1371/journal.pone.0044880.g010 Supporting Information Figure S1 The induction of pro-inflammatory cytokines in serums and livers after APAP challenge in mice. (A) The serum levels of IL-1b, TNFa and IFNc and (B) hepatic protein levels of IL-1b and IFNc at 8 hr after treatment with APAP. *P,0.05; **P,0.01; ***P,0.001. Data are mean 6 SEM from 6,8 mice per group. (TIF) Figure S2 The number of infiltrated hepatic neutrophils is greater in Il152/2 mice after APAP injection. Hepatic non-parenchymal cells were isolated from APAP-injected WT and Il152/2 mice. Cells were stained for CD11b and F4/80 cell surface markers. CD11b+ cells were gated to demonstrate in dot plots. (A) The relative percentages of neutrophils and KCs in CD11b+ cells at 0 and 8 hr after APAP injection, dot plots and numbers are the representative data. (B) Data are from 3,5 mice at 0 and 8 hr, respectively, after APAP challenge. *P,0.05. Data are mean 6 SEM from 3,5 mice per group. (TIF) Figure S3 The level of hepatic Hsp70 is lower in Il152/2 mice after APAP challenge. The Hsp70/HSC70 levels were evaluated by quantitative PCR and western blot analysis. mRNA PLOS ONE | www.plosone.org 12 September 2012 | Volume 7 | Issue 9 | e44880