Multigene Expression Profiling Figure 3. Relative expression level of 20 genes in yeast across the samples induced by weak acid and that non-induced detected by MgC-GEP, real time RT-PCR and Microarray analysis. Yeast cells of S. cerevisiae W303-1A strain from overnight cultures in YPD were diluted in fresh YPD to an OD600 of 0.1 and grown at 30uC until an OD600 of 1–1.1 was reached. Cultures were split and potassium sorbate (BBI) was added at a final concentration of 8 mM to one half of the culture. After 20 min, both untreated and treated cultures were harvested by centrifugation at room temperature (2 min, 40006g), and cells were immediately washed in ice-cold water, reharvested at 4uC, snap-frozen in liquid nitrogen and saved at 280uC. *Microarray data was cited from previous research [40]. doi:10.1371/journal.pone.0015711.g003 Table S2 (DOC) References Supporting Information Table S1 Multiplex gene-specific primers and universal primers. *Underlined sequences are restriction sites. Italic letters are the universal sequences. (DOC) Primer sequence for real-time RT-PCR. Table S3 The amplification efficiencies of specific prim- ers. The amplification efficiencies of specific primers were determined using real-time RT-PCR described in Materials and Methods. (DOC) Author Contributions Conceived and designed the experiments: YX. Performed the experiments: KJ XZ. Analyzed the data: KJ XZ. Contributed reagents/materials/ analysis tools: YX. Wrote the paper: KJ XZ YX. 1. Gregory SG, Sekhon M, Schein J, Zhao S, Osoegawa K, et al. (2002) A physical map of the mouse genome. Nature 418: 743–750. 2. Li R, Fan W, Tian G, Zhu H, He L, et al. (2009) The sequence and de novo assembly of the giant panda genome. Nature 463: 311–317. 3. Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, et al. (2001) Initial sequencing and analysis of the human genome. Nature 409: 860–921. 4. Dean RA, Talbot NJ, Ebbole DJ, Farman ML, Mitchell TK, et al. (2005) The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434: 980–986. 5. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, et al. (2000) The genome sequence of Drosophila melanogaster. Science 287: 2185–2195. 6. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, et al. (2002) The genome sequence of the malaria mosquito Anopheles gambiae. Science 298: 129–149. 7. Sasaki T, Matsumoto T, Yamamoto K, Sakata K, Baba T, et al. (2002) The genome sequence and structure of rice chromosome 1. Nature 420: 312–316. 8. Nene V, Wortman JR, Lawson D, Haas B, Kodira C, et al. (2007) Genome sequence of Aedes aegypti, a major arbovirus vector. Science 316: 1718–1723. 9. Hughes TR, Marton MJ, Jones AR, Roberts CJ, Stoughton R, et al. (2000) Functional discovery via a compendium of expression profiles. Cell 102: 109–126. 10. Cebeci O, Budak H (2009) Global expression patterns of three Festuca species exposed to different doses of glyphosate using the affymetrix geneChip wheat genome array. Comp Funct Genomics 2009: 505701. 11. Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, et al. (2009) Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci USA 106: 21984–21989. 12. Ji RR, de Silva H, Jin Y, Bruccoleri RE, Cao J, et al. (2009) Transcriptional profiling of the dose response: a more powerful approach for characterizing drug activities. PLoS Comput Biol 5: e1000512. 13. Hu G, Agarwal P (2009) Human disease-drug network based on genomic expression profiles. PLoS One 4: e6536. 14. Golub TR, Slonim DK, Tamayo P, Huard C, Gaasenbeek M, et al. (1999) Molecular classification of cancer: class discovery and class prediction by gene expression monitoring. Science 286: 531–537. 15. Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, et al. (2000) Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403: 503–511. PLoS ONE | www.plosone.org 5 January 2011 | Volume 6 | Issue 1 | e15711