Redundancy in Plant Defense Figure 1. Morphological, molecular, and defense phenotypes of ssi2 ndr1-1 sid2-1 and ssi2 eds1-1 sid2-1 plants. (A) Comparison of the morphological phenotypes displayed by 3-week-old soil-grown plants (scale, 0.5 cm). (B) Microscopy of trypan blue-stained leaves from wt (SSI2, Col- 0 ecotype), ssi2, ssi2 eds1-1, ssi2 sid2-1 and ssi2 eds1-1 sid2-1 plants (scale bars, 270 microns). (C) SA and SAG levels in indicated genotypes. The error bars indicate SD. Asterisks indicate data statistically significant from wt N o¨ ecotype (SSI2) (P,0.05, n = 4). (D) Expression of PR-1 and PR-2 genes in indicated genotypes. Total RNA was extracted from 4-week-old plants and used for RNA gel-blot analysis. Ethidium bromide staining of rRNA was used as the loading control. The PR-1 transcript levels in EDS1 SID2 F2 plants were similar to those of wt plants (data not shown). (E) RT-PCR analysis of various R genes in indicated genotypes. The level of b-tubulin was used as an internal control to normalize the amount of cDNA template. (F) RT-PCR analysis of various R genes in indicated genotypes. The level of b-tubulin was used as an internal control to normalize the amount of cDNA template. The expression of R genes in EDS1 SID2 F2 plants was similar to that of wt plants (data not shown). (G) Levels of Myc-tagged RPM1 protein in indicated genotypes. Levels of Rubisco were used as the loading control. doi:10.1371/journal.pgen.1000545.g001 PLoS Genetics | www.plosgenetics.org 3 July 2009 | Volume 5 | Issue 7 | e1000545