HSPG Regulates Trans-Synaptic Signaling Figure 2. Loss of sulf1/hs6st causes opposite effects on transmission strength. (A) Representative excitatory junctional current (EJC) traces from control (w11186UH1-GAL4), sulf1 RNAi (UH1-GAL46UAS-CG6725) and hs6st RNAi (UH1-GAL46UAS-CG4451). The nerve was stimulated (arrows) in 1.0 mM external Ca2+, with TEVC records (260 mV holding potential) from muscle 6 in segment A3. Each trace averaged from 10 consecutive recordings. (B) Quantified mean EJC amplitudes (nA) for the three genotypes shown in panel A. (C) Representative traces from control (w1118), sulf1D1 and hs6std770 alleles under the same conditions described in panel A. (D) Quantified mean EJC amplitudes (nA) for the three genotypes shown in panel C. Sample sizes are at least 11 animals per indicated genotype. Statistically significant differences calculated using student’s t-test, ** p,0.01, *** p,0.001. Error bars indicate S.E.M. doi:10.1371/journal.pgen.1003031.g002 PLOS Genetics | www.plosgenetics.org 6 November 2012 | Volume 8 | Issue 11 | e1003031