levels of (A) Hsp70 and (B) HSC70, and (C) protein levels and quantification data of Hsp70/HSC70 at 8 hr post-APAP injection in mice. #P,0.05, compared with WT at 0 hr; *P,0.05; **P,0.01. Data are mean 6 SEM from 5,8 mice per group. (TIF) Figure S4 Effect of Nnt mutation on APAP-induced hepatitis in WT or Il152/2 mice. (A) Nnt genotyping results of WT and Il152/2 mice. DNA samples from mice were evaluated with Nnt+/+ (wt) and Nnt2/2 (mut) genetic markers by PCR. (B) Serum levels of ALT at 8 hr after APAP treatment in WT and Il152/2 mice. *P,0.05; **P,0.01; **P,0.001. Data are mean 6 SEM from 5,8 mice per group. (TIF) Methods S1 Determination of NPC sub-population with flow cytometry, and Nnt genotyping of the mice. (DOC) References 1. Dahlin DC, Miwa GT, Lu AY, Nelson SD (1984) N-acetyl-p-benzoquinone imine: a cytochrome P-450-mediated oxidation product of acetaminophen. Proc Natl Acad Sci U S A 81: 1327–1331. 2. 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