Menin Is Phosphorylated in Response to DNA Damage Figure 3. Menin is enriched at additional DNA damage responsive genes after DNA damage. Quantitative RT-PCR results showing induction of GADD45A (A) and MDM2 (D) mRNA 0.5 h or 6h following treatment with 1000 Rads of c-IR or 10J/m2 UV in U2OS cells. ChIPs were performed in U2OS cells under the above conditions. The association of menin, p53 and RNAPII with the GADD45A locus was quantitated following treatment with 1000 Rads of c-IR (B) or 10J/m2 UV (C). ChIP of menin, p53, and RNAPII along the MDM2 locus after IR (E) or UV (F). Graphs represent PLoS ONE | www.plosone.org 5 January 2011 | Volume 6 | Issue 1 | e16119