TRPV4 Regulates Cytoskeleton Figure 3. TRPV4 co-localizes with actin and microtubule cytoskeleton. a–c. Shown are the live-cell confocal images of F11 cells expressing TRV4-GFP (green) and RFP-actin (red). Presences of TRPV4-GFP specifically in actin-enriched structures are shown. a. Enlarged view of lamellipodia and at the tip of the actin filaments are shown. b–c. Enlarged view of focal adhesion point-like structures (b) and cell cortex with actin ribs (c) are shown. Arrows indicate the localization of TRPV4-GFP at filopodial tips. d–f. TRPV4 co-localizes with microtubule cytoskeleton. Shown are the confocal images of F11 cells immunostained for TRPV4 (green) and tyrosinated tubulin (red). Arrows indicate presence and acumulation of microtubules in thin filopodial structures (d, upper panel) and thin lamellipodial structures (e, middle panel). The status of the microtubules in the non-transfected cells are shown in below (f, lower panel). doi:10.1371/journal.pone.0011654.g003 PLoS ONE | www.plosone.org 5 July 2010 | Volume 5 | Issue 7 | e11654