Redundancy in Plant Defense Figure 2. Restoration of ssi2 phenotypes in ssi2 eds1-1 sid2-1 plants and glycerol responsiveness of eds1-1 sid2-1 plants. (A) Visual phenotypes of water- or BTH–treated wt (SSI2; Col-0 ecotype) and ssi2 eds1-1 sid2-1 plants. The plants were photographed at 2 days post treatment (dpt). (B) Microscopy of trypan blue-stained leaves from BTH–treated wt (SSI2; Col-0 ecotype), sid2, eds1-1 and ssi2 eds1-1 sid2-1 plants. The plants were treated with BTH and stained at 2 dpt (scale bars, 270 microns). (C) RT–PCR analysis of R genes in water- or BTH-treated ssi2 eds1-1 sid2-1 plants. Untreated wt (SSI2; Col-0 ecotype) and ssi2 plants were used as controls. The expression of R genes in EDS1 SID2 F2 plants was similar to that of wt plants (data not shown). The level of b-tubulin was used as an internal control to normalize the amount of cDNA template. (D) RT–PCR analysis of various R genes in water- or glycerol-treated sid2-1 and eds1-1 sid2-1 plants. The glycerol-treated wt (SSI2; Col-0 ecotype) and eds1-1 were included as additional controls. The expression of R genes in water- or glycerol-treated EDS1 SID2 F2 plants was similar to that of water- or glycerol-treated wt plants, respectively (data not shown). The expression of R genes in wt and eds1-1 plants was similar to that seen in sid2-1 or eds1-1 sid2-1 plants. The plants were treated with water or glycerol for three days and analyzed for 18:1 levels and R gene expression. The level of b-tubulin was used as an internal control to normalize the amount of cDNA template. The 18:1 content of each genotype is shown as mol%6SD. doi:10.1371/journal.pgen.1000545.g002 PLoS Genetics | www.plosgenetics.org 5 July 2009 | Volume 5 | Issue 7 | e1000545