Mtmr8 in Muscle Development Figure 4. PH/G domain of Mtmr8 is required to cooperate with PI3K regulating embryo development and essential for the phosphatase activity of zebrafish Mtmr8 gene. (A) Splice junction morpholino targeted against Mtmr8 exon-intron boundary resulted to loss of PH/G domain. RT-PCR of Mtmr8 transcript at 24hpf in WT and Mtmr8-MO2 (6 ng) morpholino-injected embryos, comparing cryptic spliced transcript in the morpholino injected embryos to the cDNA PCR products. (B) Embryo phenotypes observed under the microscope. LY represents the PI3K PLoS ONE | www.plosone.org 6 March 2009 | Volume 4 | Issue 3 | e4979