Mtmr8 in Muscle Development Figure 6. The effect of Mtmr8 knockdown on hedgehog pathway and slow muscle development. (A) The expression of myod and ptc1 at 24hpf in the Cont MO (a, d), Mtmr8-MO1 morphant (b, e) and it injected with dnPKA mRNA (c, f). (B) Whole-mount staining of 24 hpf embryo with F59 antibody (green) and Prox1 antibody (red) and to reveal slow muscle cells. The pictures are representative of at least three experiments (30 embryos in each experiment). All scale bars are 100 mm. doi:10.1371/journal.pone.0004979.g006 Figure 7. Mtmr8 controls actin modeling non-cell-autonomously. (A) Schematic depiction of cell transplant experiments. (B) Representative picture of a chimeric embryo at 24 hpf. The labeling cells were mostly located in somites of trunk and tail regions. (C) The effects of cell transplantation on F-actin. doi:10.1371/journal.pone.0004979.g007 PLoS ONE | www.plosone.org 9 March 2009 | Volume 4 | Issue 3 | e4979