Chromatin immunoprecipitation and PCR. Chromatin immunoprecipitation was performed according to the published protocol [35]. Polyclonal antibody to AR (PG-21, Upstate, NY, USA) was used for immunoprecipitation on the control (vehicle treated) and androgen treated LNCaP cell nuclear lysates. For PCR analysis of target gene enrichment analysis, 2 ml of input DNA, and target-enriched DNA were used. PCR primer sequences designed to flank the target gene promoters and used for ChIP-PCR are given in Supplementary Table S5. Supporting Information. Two supplementary figures, and five supplementary tables. This material is available free at http:// pubs.plosone.org. Supporting Information Figure S1 Found at: doi:10.1371/journal.pone.0007075.s001 (0.27 PNG) Figure S2 Found at: doi:10.1371/journal.pone.0007075.s002 (0.76 PNG) Table S1 Found at: doi:10.1371/journal.pone.0007075.s003 (0.01 PDF) Table S2 Found at: doi:10.1371/journal.pone.0007075.s004 (0.01 PDF) MB MB MB MB References 1. 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PLoS ONE | www.plosone.org 11 September 2009 | Volume 4 | Issue 9 | e7075