Mtmr8 in Muscle Development Figure 5. Mtmr8 cooperates with PI3K regulating actin modeling. (A) Staining of embryos with phalloidin (F-actin) at 24hpf. (B) Nexilin mRNA expression at 24 hpf. (C) RT-PCR of Nexilin transcript at 24hpf in WT and Nexilin-MO (3 ng) morpholino-injected embryos, comparing cryptic spliced transcript in the morpholino injected embryos to the cDNA PCR products. (D) The effects of Nexilin knockdown on embryo development (a, b) and F- actin (c, d). The pictures are representative of at least three experiments (30 embryos in each experiment). All scale bars are 100 mm. doi:10.1371/journal.pone.0004979.g005 PLoS ONE | www.plosone.org 8 March 2009 | Volume 4 | Issue 3 | e4979