5. Holscher C, Reiling N, Schaible UE, Holscher A, Bathmann C, et al. (2008) Containment of aerogenic Mycobacterium tuberculosis infection in mice does not require MyD88 adaptor function for TLR2, -4 and -9. Eur J Immunol 38: 680–694. 6. Reiling N, Holscher C, Fehrenbach A, Kroger S, Kirschning CJ, et al. (2002) Cutting edge: Toll-like receptor (TLR)2- and TLR4-mediated pathogen recognition in resistance to airborne infection with Mycobacterium tuberculosis. J Immunol 169: 3480–3484. 7. Reiling N, Klug K, Krallmann-Wenzel U, Laves R, Goyert S, et al. (2001) Complex encounters at the macrophage-mycobacterium interface: studies on the role of the mannose receptor and CD14 in experimental infection models with Mycobacterium avium. Immunobiology 204: 558–571. 8. Schafer G, Brown GD (2009) Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors. Methods Mol Biol 531: 1–15. 9. Peiser L, Gordon S (2001) The function of scavenger receptors expressed by macrophages and their role in the regulation of inflammation. Microbes Infect 3: 149–159. 10. Neyrolles O, Hernandez-Pando R, Pietri-Rouxel F, Fornes P, Tailleux L, et al. (2006) Is adipose tissue a place for Mycobacterium tuberculosis persistence? PLoS ONE 1: e43. 11. Bowdish DM, Sakamoto K, Kim MJ, Kroos M, Mukhopadhyay S, et al. (2009) MARCO, TLR2, and CD14 are required for macrophage cytokine responses to mycobacterial trehalose dimycolate and Mycobacterium tuberculosis. PLoS Pathog 5: e1000474. 12. Acton S, Rigotti A, Landschulz KT, Xu S, Hobbs HH, et al. (1996) Identification of scavenger receptor SR-BI as a high density lipoprotein receptor. Science 271: 518–520. 13. Rhainds D, Brissette L (2004) The role of scavenger receptor class B type I (SR- BI) in lipid trafficking. defining the rules for lipid traders. Int J Biochem Cell Biol 36: 39–77. 14. Connelly MA, Williams DL (2004) Scavenger receptor BI: a scavenger receptor with a mission to transport high density lipoprotein lipids. Curr Opin Lipidol 15: 287–295. 15. Fidge NH (1999) High density lipoprotein receptors, binding proteins, and ligands. J Lipid Res 40: 187–201. 16. Acton SL, Scherer PE, Lodish HF, Krieger M (1994) Expression cloning of SR- BI, a CD36-related class B scavenger receptor. J Biol Chem 269: 21003–21009. 17. Rigotti A, Acton SL, Krieger M (1995) The class B scavenger receptors SR-BI and CD36 are receptors for anionic phospholipids. J Biol Chem 270: 16221–16224. 18. Calvo D, Gomez-Coronado D, Lasuncion MA, Vega MA (1997) CLA-1 is an 85-kD plasma membrane glycoprotein that acts as a high-affinity receptor for both native (HDL, LDL, and VLDL) and modified (OxLDL and AcLDL) lipoproteins. Arterioscler Thromb Vasc Biol 17: 2341–2349. 19. Philips JA, Rubin EJ, Perrimon N (2005) Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection. Science 309: 1251–1253. 20. Vishnyakova TG, Kurlander R, Bocharov AV, Baranova IN, Chen Z, et al. (2006) CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells. Proc Natl Acad Sci U S A 103: 16888–16893. 21. Voisset C, Callens N, Blanchard E, Op De Beeck A, Dubuisson J, et al. (2005) High density lipoproteins facilitate hepatitis C virus entry through the scavenger receptor class B type I. J Biol Chem 280: 7793–7799. 22. Catanese MT, Graziani R, von Hahn T, Moreau M, Huby T, et al. (2007) High-avidity monoclonal antibodies against the human scavenger class B type I receptor efficiently block hepatitis C virus infection in the presence of high- density lipoprotein. J Virol 81: 8063–8071. 23. Rodrigues CD, Hannus M, Prudencio M, Martin C, Goncalves LA, et al. (2008) Host scavenger receptor SR-BI plays a dual role in the establishment of malaria parasite liver infection. Cell Host Microbe 4: 271–282. 24. Brown GD, Gordon S (2001) Immune recognition. A new receptor for beta- glucans. Nature 413: 36–37. 25. Taylor PR, Brown GD, Herre J, Williams DL, Willment JA, et al. (2004) The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages. J Immunol 172: 1157–1162. 26. Gatfield J, Pieters J (2000) Essential role for cholesterol in entry of mycobacteria into macrophages. Science 288: 1647–1650. 27. Stokes RW, Thorson LM, Speert DP (1998) Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficient. J Immunol 160: 5514–5521. 28. Martens GW, Arikan MC, Lee J, Ren F, Vallerskog T, et al. (2008) Hypercholesterolemia impairs immunity to tuberculosis. Infect Immun 76: 3464–3472. M. tuberculosis and SR-B1 29. Kellner-Weibel G, de La Llera-Moya M, Connelly MA, Stoudt G, Christian AE, et al. (2000) Expression of scavenger receptor BI in COS-7 cells alters cholesterol content and distribution. Biochemistry 39: 221–229. 30. Baranova I, Vishnyakova T, Bocharov A, Chen Z, Remaley AT, et al. (2002) Lipopolysaccharide down regulates both scavenger receptor B1 and ATP binding cassette transporter A1 in RAW cells. Infect Immun 70: 2995–3003. 31. Randhawa AK, Ziltener HJ, Merzaban JS, Stokes RW (2005) CD43 is required for optimal growth inhibition of Mycobacterium tuberculosis in macrophages and in mice. J Immunol 175: 1805–1812. 32. Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, et al. (2007) A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Proc Natl Acad Sci U S A 104: 1947–1952. 33. Pieters J (2008) Mycobacterium tuberculosis and the macrophage: maintaining a balance. Cell Host Microbe 3: 399–407. 34. Schlesinger LS (1993) Macrophage phagocytosis of virulent but not attenuated strains of Mycobacterium tuberculosis is mediated by mannose receptors in addition to complement receptors. J Immunol 150: 2920–2930. 35. Bulut Y, Michelsen KS, Hayrapetian L, Naiki Y, Spallek R, et al. (2005) Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals. J Biol Chem 280: 20961–20967. 36. Yadav M, Schorey JS (2006) The beta-glucan receptor dectin-1 functions together with TLR2 to mediate macrophage activation by mycobacteria. Blood 108: 3168–3175. 37. Le Cabec V, Carreno S, Moisand A, Bordier C, Maridonneau-Parini I (2002) Complement receptor 3 (CD11b/CD18) mediates type I and type II phagocytosis during nonopsonic and opsonic phagocytosis, respectively. J Immunol 169: 2003–2009. 38. Jo EK, Yang CS, Choi CH, Harding CV (2007) Intracellular signalling cascades regulating innate immune responses to Mycobacteria: branching out from Toll- like receptors. Cell Microbiol 9: 1087–1098. 39. Feng CG, Scanga CA, Collazo-Custodio CM, Cheever AW, Hieny S, et al. (2003) Mice lacking myeloid differentiation factor 88 display profound defects in host resistance and immune responses to Mycobacterium avium infection not exhibited by Toll-like receptor 2 (TLR2)- and TLR4-deficient animals. J Immunol 171: 4758–4764. 40. Drennan MB, Nicolle D, Quesniaux VJ, Jacobs M, Allie N, et al. (2004) Toll-like receptor 2-deficient mice succumb to Mycobacterium tuberculosis infection. Am J Pathol 164: 49–57. 41. Abel B, Thieblemont N, Quesniaux VJ, Brown N, Mpagi J, et al. (2002) Toll-like receptor 4 expression is required to control chronic Mycobacterium tuberculosis infection in mice. J Immunol 169: 3155–3162. 42. Stokes RW, Norris-Jones R, Brooks DE, Beveridge TJ, Doxsee D, et al. (2004) The glycan-rich outer layer of the cell wall of Mycobacterium tuberculosis acts as an antiphagocytic capsule limiting the association of the bacterium with macrophages. Infect Immun 72: 5676–5686. 43. Hall-Stoodley L, Watts G, Crowther JE, Balagopal A, Torrelles JB, et al. (2006) Mycobacterium tuberculosis binding to human surfactant proteins A and D, fibronectin, and small airway epithelial cells under shear conditions. Infect Immun 74: 3587–3596. 44. Ferguson JS, Voelker DR, McCormack FX, Schlesinger LS (1999) Surfactant protein D binds to Mycobacterium tuberculosis bacilli and lipoarabinomannan via carbohydrate-lectin interactions resulting in reduced phagocytosis of the bacteria by macrophages. J Immunol 163: 312–321. 45. Ferguson JS, Voelker DR, Ufnar JA, Dawson AJ, Schlesinger LS (2002) Surfactant protein D inhibition of human macrophage uptake of Mycobacte- rium tuberculosis is independent of bacterial agglutination. J Immunol 168: 1309–1314. 46. Sanchez MD, Garcia Y, Montes C, Paris SC, Rojas M, et al. (2006) Functional and phenotypic changes in monocytes from patients with tuberculosis are reversed with treatment. Microbes Infect 8: 2492–2500. 47. Freeman AE, Price PJ, Igel HJ, Young JC, Maryak JM, et al. (1970) Morphological transformation of rat embryo cells induced by diethylnitrosamine and murine leukemia viruses. J Natl Cancer Inst 44: 65–78. 48. Morita S, Kojima T, Kitamura T (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7: 1063–1066. 49. Snewin VA, Gares MP, Gaora PO, Hasan Z, Brown IN, et al. (1999) Assessment of immunity to mycobacterial infection with luciferase reporter constructs. Infect Immun 67: 4586–4593. 50. Rigotti A, Trigatti BL, Penman M, Rayburn H, Herz J, et al. (1997) A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. Proc Natl Acad Sci U S A 94: 12610–12615. 51. Trigatti B, Rayburn H, Vinals M, Braun A, Miettinen H, et al. (1999) Influence of the high density lipoprotein receptor SR-BI on reproductive and cardiovascular pathophysiology. Proc Natl Acad Sci U S A 96: 9322–9327. PLoS ONE | www.plosone.org 13 December 2009 | Volume 4 | Issue 12 | e8448