Menin Is Phosphorylated in Response to DNA Damage Figure 2. Menin is associated with the CDKN1A locus after DNA damage. (A) Quantitative RT-PCR results showing induction of CDKN1A mRNA 0.5 h or 6 h following treatment with 1000 Rads of c-IR or 10J/m2 UV in U2OS cells. (B) Whole cell extracts were prepared from U2OS cells under the above conditions and immunoblotted with the indicated antibodies. ChIPs were performed from U2OS cells following treatment with 1000 Rads of c-IR (C) or 10J/m2 UV (D). p53 (left panel), menin (middle panel) and RNAPII (right panel) were quantitated along the CDKN1A locus. Results are the average of at least 3independent experiments. Error bars are standard error of the mean. * p,0.05. doi:10.1371/journal.pone.0016119.g002 PLoS ONE | www.plosone.org 4 January 2011 | Volume 6 | Issue 1 | e16119