HORMAD1 Is Essential for Male and Female Meiosis Figure 8. Sex chromosome expressed transcripts escape meiotic sex chromosome inactivation in Hormad 2/2 spermatocytes. (A–H) Quantitative real time PCR analyses show that expression of testes specific genes derived from the autosome, Hormad2, Rnh2, and Mov10l1, did not significantly differ between the wild-type and the Hormad12/2 testes (A-C). However, X chromosome linked, testis specific genes, Usp26, Fthl17, Pramel3, Tex11 and Tex13 transcript were 2–6 fold increased (D-H). (I) Microarray analysis shows preponderance of X and Y derived transcripts among the top 38 up-regulated genes in Hormad 2/2 testes. Data are normalized to b-actin expression and presented as the mean relative quantity (compared to wild-type) with error bars representing the standard error of mean. Student’s t test was used to calculate the P values. doi:10.1371/journal.pgen.1001190.g008 PLoS Genetics | www.plosgenetics.org 10 November 2010 | Volume 6 | Issue 11 | e1001190