2 mg, 10%). Lane 6: GST-SMARCB1 (input, 2 mg, 10%). Lane 7: His6-NEIL1 (input, 2 mg, 13%). Note that spontaneous cleavage occurred in the GST-SMARCB1 protein sample (Lane 5). (TIF) Acknowledgments The confocal imaging was performed at the Molecular Imaging Center (Fuge, Norwegian Research Council), University of Bergen, Norway. References 1. Adler SP, Nigro G, Pereira L (2007) Recent advances in the prevention and treatment of congenital cytomegalovirus infections. Semin Perinatol 31: 10–18. 2. Kenneson A, Cannon MJ (2007) Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection. Rev Med Virol 17: 253–276. 3. Emery VC (2001) Investigation of CMV disease in immunocompromised patients. J Clin Pathol 54: 84–88. 4. Hassan-Walker AF, Kidd IM, Sabin C, Sweny P, Griffiths PD, et al. (1999) Quantity of human cytomegalovirus (CMV) DNAemia as a risk factor for CMV disease in renal allograft recipients: relationship with donor/recipient CMV serostatus, receipt of augmented methylprednisolone and antithymocyte globulin (ATG). J Med Virol 58: 182–187. 5. Sagedal S, Nordal KP, Hartmann A, Degre M, Holter E, et al. (2000) A prospective study of the natural course of cytomegalovirus infection and disease in renal allograft recipients. Transplantation 70: 1166–1174. 6. Chee MS, Bankier AT, Beck S, Bohni R, Brown CM, et al. (1990) Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol 154: 125–169. 7. Ogawa-Goto K, Tanaka K, Gibson W, Moriishi E, Miura Y, et al. (2003) Microtubule network facilitates nuclear targeting of human cytomegalovirus capsid. J Virol 77: 8541–8547. 8. Cuevas-Bennett C, Shenk T (2008) Dynamic histone H3 acetylation and methylation at human cytomegalovirus promoters during replication in fibroblasts. J Virol 82: 9525–9536. 9. Nitzsche A, Paulus C, Nevels M (2008) Temporal dynamics of cytomegalovirus chromatin assembly in productively infected human cells. J Virol 82: 11167–11180. 10. Everett RD, Chelbi-Alix MK (2007) PML and PML nuclear bodies: implications in antiviral defence. Biochimie 89: 819–830. 11. Tavalai N, Stamminger T (2008) New insights into the role of the subnuclear structure ND10 for viral infection. Biochim Biophys Acta 1783: 2207–2221. 12. Davison AJ, Dolan A, Akter P, Addison C, Dargan DJ, et al. (2003) The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomeg- alovirus genome. J Gen Virol 84: 17–28. 13. Pari GS (2008) Nuts and bolts of human cytomegalovirus lytic DNA replication. Curr Top Microbiol Immunol 325: 153–166. 14. Chen R, Wang H, Mansky LM (2002) Roles of uracil-DNA glycosylase and dUTPase in virus replication. J Gen Virol 83: 2339–2345. 15. Courcelle CT, Courcelle J, Prichard MN, Mocarski ES (2001) Requirement for uracil-DNA glycosylase during the transition to late-phase cytomegalovirus DNA replication. J Virol 75: 7592–7601. 16. Prichard MN, Lawlor H, Duke GM, Mo C, Wang Z, et al. (2005) Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA. Virol J 2: 55. 17. Lieberman PM (2006) Chromatin regulation of virus infection. Trends in Microbiology 14: 132–140. 18. Nevels M, Nitzsche A, Paulus C (2011) How to control an infectious bead string: nucleosome-based regulation and targeting of herpesvirus chromatin. Rev Med Virol 21: 154–180. 19. Paulus C, Nitzsche A, Nevels M (2010) Chromatinisation of herpesvirus genomes. Rev Med Virol 20: 34–50. 20. Sinclair J, Sissons P (2006) Latency and reactivation of human cytomegalovirus. J Gen Virol 87: 1763–1779. 21. Sinclair J (2010) Chromatin structure regulates human cytomegalovirus gene expression during latency, reactivation and lytic infection. Biochim Biophys Acta 1799: 286–295. 22. Bannister AJ, Kouzarides T (2011) Regulation of chromatin by histone modifications. Cell Res 21: 381–395. 23. Hargreaves DC, Crabtree GR (2011) ATP-dependent chromatin remodeling: genetics, genomics and mechanisms. Cell Res 21: 396–420. 24. Narlikar GJ, Fan HY, Kingston RE (2002) Cooperation between complexes that regulate chromatin structure and transcription. Cell 108: 475–487. 25. Clapier CR, Cairns BR (2009) The biology of chromatin remodeling complexes. Annu Rev Biochem 78: 273–304. 26. Ranneberg-Nilsen T, Dale HA, Luna L, Slettebakk R, Sundheim O, et al. (2008) Characterization of human cytomegalovirus uracil DNA glycosylase (UL114) and its interaction with polymerase processivity factor (UL44). J Mol Biol 381: 276–288. Human Cytomegalovirus UL114 Interact with SMARCB1 Author Contributions Conceived and designed the experiments: TRN HR RS LL MB. Performed the experiments: TRN RS PHB LL ØO. Analyzed the data: TRN HR LL MB. Contributed reagents/materials/analysis tools: HR MB. Wrote the paper: TRN HR LL MB. 27. Penfold ME, Mocarski ES (1997) Formation of cytomegalovirus DNA replication compartments defined by localization of viral proteins and DNA synthesis. Virology 239: 46–61. 28. Phelan ML, Sif S, Narlikar GJ, Kingston RE (1999) Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Mol Cell 3: 247–253. 29. Kingston RE, Bunker CA, Imbalzano AN (1996) Repression and activation by multiprotein complexes that alter chromatin structure. Genes Dev 10: 905–920. 30. Sudarsanam P, Winston F (2000) The Swi/Snf family nucleosome-remodeling complexes and transcriptional control. Trends Genet 16: 345–351. 31. Reyes JC, Muchardt C, Yaniv M (1997) Components of the human SWI/SNF complex are enriched in active chromatin and are associated with the nuclear matrix. J Cell Biol 137: 263–274. 32. Maxwell KL, Frappier L (2007) Viral proteomics. Microbiol Mol Biol Rev 71: 398–411. 33. Oh J, Fraser NW (2008) Temporal association of the herpes simplex virus genome with histone proteins during a lytic infection. J Virol 82: 3530–3537. 34. Varnum SM, Streblow DN, Monroe ME, Smith P, Auberry KJ, et al. (2004) Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome. J Virol 78: 10960–10966. 35. Huang J, Kent JR, Placek B, Whelan KA, Hollow CM, et al. (2006) Trimethylation of histone H3 lysine 4 by Set1 in the lytic infection of human herpes simplex virus 1. J Virol 80: 5740–5746. 36. Ioudinkova E, Arcangeletti MC, Rynditch A, De CF, Motta F, et al. (2006) Control of human cytomegalovirus gene expression by differential histone modifications during lytic and latent infection of a monocytic cell line. Gene 384: 120–128. 37. Kent JR, Zeng PY, Atanasiu D, Gardner J, Fraser NW, et al. (2004) During lytic infection herpes simplex virus type 1 is associated with histones bearing modifications that correlate with active transcription. J Virol 78: 10178–10186. 38. Murphy JC, Fischle W, Verdin E, Sinclair JH (2002) Control of cytomegalovirus lytic gene expression by histone acetylation. EMBO J 21: 1112–1120. 39. Wang W (2003) The SWI/SNF family of ATP-dependent chromatin remodelers: similar mechanisms for diverse functions. Curr Top Microbiol Immunol 274: 143–169. 40. Kalpana GV, Marmon S, Wang W, Crabtree GR, Goff SP (1994) Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science 266: 2002–2006. 41. Cha S, Seo T (2011) hSNF5 is required for human papillomavirus E2-driven transcriptional activation and DNA replication. Intervirology 54: 66–77. 42. Kumar RA, Naidu SR, Wang X, Imbalzano AN, Androphy EJ (2007) Interaction of papillomavirus E2 protein with the Brm chromatin remodeling complex leads to enhanced transcriptional activation. J Virol 81: 2213–2220. 43. Lee D, Sohn H, Kalpana GV, Choe J (1999) Interaction of E1 and hSNF5 proteins stimulates replication of human papillomavirus DNA. Nature 399: 487–491. 44. Wu DY, Kalpana GV, Goff SP, Schubach WH (1996) Epstein-Barr virus nuclear protein 2 (EBNA2) binds to a component of the human SNF-SWI complex, hSNF5/Ini1. J Virol 70: 6020–6028. 45. Hwang S, Lee D, Gwack Y, Min H, Choe J (2003) Kaposi’s sarcoma-associated herpesvirus K8 protein interacts with hSNF5. J Gen Virol 84: 665–676. 46. Herrera FJ, Triezenberg SJ (2004) VP16-dependent association of chromatin- modifying coactivators and underrepresentation of histones at immediate-early gene promoters during herpes simplex virus infection. J Virol 78: 9689–9696. 47. Taylor TJ, Knipe DM (2004) Proteomics of herpes simplex virus replication compartments: association of cellular DNA replication, repair, recombination, and chromatin remodeling proteins with ICP8. J Virol 78: 5856–5866. 48. Martens JA, Winston F (2003) Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr Opin Genet Dev 13: 136–142. 49. Cihlar T, Fuller MD, Cherrington JM (1997) Expression of the catalytic subunit (UL54) and the accessory protein (UL44) of human cytomegalovirus DNA polymerase in a coupled in vitro transcription/translation system. Protein Expr Purif 11: 209–218. 50. Ertl PF, Powell KL (1992) Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells. J Virol 66: 4126–4133. 51. Weiland KL, Oien NL, Homa F, Wathen MW (1994) Functional analysis of human cytomegalovirus polymerase accessory protein. Virus Res 34: 191–206. PLoS ONE | www.plosone.org 11 March 2012 | Volume 7 | Issue 3 | e34119