PAGE 13 OF 13 Nucleic Acids Research, 2008, Vol. 36, No. 7 e42 45. Worm,J., Aggerholm,A. and Guldberg,P. (2001) In-tube DNA methylation proﬁling by ﬂuorescence melting curve analysis. Clin. Chem., 47, 1183–1189. 46. Warnecke,P.M., Stirzaker,C., Melki,J.R., Millar,D.S., Paul,C.L. and Clark,S.J. (1997) Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA. Nucleic Acids Res., 25, 4422–4426. 47. Akey,D.T., Akey,J.M., Zhang,K. and Jin,L. (2002) Assaying DNA methylation based on high-throughput melting curve approaches. Genomics, 80, 376–384. 48. Wojdacz,T.K. and Dobrovic,A. (2007) Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res., 35, e41.