1734 Nucleic Acids Research, 2010, Vol. 38, No. 5 Figure 10. A PROMALS3D sequence alignment of KlcA and ArdB proteins. KlcA_RK2 and ArdB_pKM101 are the archetypal sequences for these two proteins (11,20). Yellow shading represents beta strands, red shading represents alpha helices and purple shading represents the 310 helix. The grey shading highlights the anti-restriction motif LLREYVNTL in ArdB from pKM101 (7,26). Consensus amino acid symbols are: conserved amino acids are in bold and uppercase letters; aliphatic (I, V, L): l; aromatic (Y, H, W, F): @; hydrophobic (W, F, Y, M, L, I, V, A, C, T, H): h; alcohol (S, T): o; polar residues (D, E, H, K, N, Q, R, S, T): p; tiny (A, G, C, S): t; small (A, G, C, S, V, N, D, T, P): s; bulky residues (E, F, I, K, L, M, Q, R, W, Y): b; positively charged (K, R, H): +; negatively charged (D, E): ; charged (D, E, K, R, H): c. Figure 11. Sequence conservation mapped onto KlcA136 protein surface. Conservation surface representation based upon the PROMALS3D multiple sequence alignment of ArdB and KlcA sequences (Supplementary Figure S5) where largely and completely conserved residues are shown in lavender/plum/violet colour mapped onto the surface and variable residue positions coloured cyan.