2716 Nucleic Acids Research, 2011, Vol. 39, No. 7 37. Mount,S.M., Burks,C., Hertz,G., Stormo,G.D., White,O. and Fields,C. (1992) Splicing signals in Drosophila: intron size, information content, and consensus sequences. Nucleic Acids Res., 20, 4255–4262. 38. Sheth,N., Roca,X., Hastings,M.L., Roeder,T., Krainer,A.R. and Sachidanandam,R. (2006) Comprehensive splice-site analysis using comparative genomics. Nucleic Acids Res., 34, 3955–3967. 39. Yeo,G. and Burge,C.B. (2004) Maximum entropy modeling of short sequence motifs with applications to RNA splicing signals. J. Comput. Biol., 11, 377–394. 40. Lareau,L.F., Green,R.E., Bhatnagar,R.S. and Brenner,S.E. (2004) The evolving roles of alternative splicing. Curr. Opin. Struct. Biol., 14, 273–282. 41. Wilson,D., Charoensawan,V., Kummerfeld,S.K. and Teichmann,S.A. (2008) DBD – taxonomically broad transcription factor predictions: new content and functionality. Nucleic Acids Res., 36(Database issue), D88–D92. 42. Hwang,S., Gou,Z. and Kuznetsov,I.B. (2007) DP-Bind: a web server for sequence-based prediction of DNA-binding residues in DNA-binding proteins. Bioinformatics, 23, 634–636. 43. Kuznetsov,I.B., Gou,Z., Li,R. and Hwang,S. (2006) Using evolutionary and structural information to predict DNA-binding sites on DNA-binding proteins. Proteins, 64, 19–27. 44. Hennig,W. (2003) Chromosomal proteins in the spermatogenesis of Drosophila. Chromosoma, 111, 489–494. 45. Bastian,F., Parmentier,G., Roux,J., Moretti,S., Laudet,V. and Robinson-Rechavi,M. (2008) Bgee: integrating and comparing heterogeneous transcriptome data among species. DILS: LNCS, 5109, 124–131. 46. Anderson,S.J., Lauritsen,J.P., Hartman,M.G., Foushee,A.M., Lefebvre,J.M., Shinton,S.A., Gerhardt,B., Hardy,R.R., Oravecz,T. and Wiest,D.L. (2007) Ablation of ribosomal protein L22 selectively impairs alphabeta T cell development by activation of a p53-dependent checkpoint. Immunity, 26, 759–772. 47. Kamath,R.S., Fraser,A.G., Dong,Y., Poulin,G., Durbin,R., Gotta,M., Kanapin,A., Le Bot,N., Moreno,S., Sohrmann,M. et al. (2003) Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421, 231–237. 48. Bourbon,H.M., Gonzy-Treboul,G., Peronnet,F., Alin,M.F., Ardourel,C., Benassayag,C., Cribbs,D., Deutsch,J., Ferrer,P., Haenlin,M. et al. (2002) A P-insertion screen identifying novel X-linked essential genes in Drosophila. Mech. Dev., 110, 71–83. 49. Boutros,M., Kiger,A.A., Armknecht,S., Kerr,K., Hild,M., Koch,B., Haas,S.A., Paro,R. and Perrimon,N. Heidelberg Fly Array Consortium. (2004) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science, 303, 832–835. 50. Deutschbauer,A.M., Jaramillo,D.F., Proctor,M., Kumm,J., Hillenmeyer,M.E., Davis,R.W., Nislow,C. and Giaever,G. (2005) Mechanisms of haploinsufﬁciency revealed by genome-wide proﬁling in yeast. Genetics, 169, 1915–1925. 51. Copley,R.R., Goodstadt,L. and Ponting,C. (2003) Eukaryotic domain evolution inferred from genome comparisons. Curr. Opin. Genet. Dev., 13, 623–628.