Biotechnol Lett (2010) 32:655–659 novel strains that co-display GFP with OPH on the yeast surface are now being developed. Acknowledgments The authors are grateful to Prof. Akihiko Kondo of Kobe University for useful discussion and the gift of Flo1p anchor system. Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. References Cortada C, Vidal L, Tejada S, Romo A, Canals A (2009) Determination of organochlorine pesticides in complex matrices by single-drop microextraction coupled to gas chromatography-mass spectrometry. Anal Chim Acta 638: 29–35 Horii K, Adachi T, Tanino T, Tanaka T, Sahara H, Shibasaki S, Ogino C, Hata Y, Ueda M, Kondo A (2009) Evaluation of cell surface-displayed protein stability against simulated gastric ﬂuid. Biotechnol Lett 31:1259–1264 Lee JK, Ahn KC, Stoutamre DW, Gee SJ, Hammock BD (2003) Development of an enzyme-linked immunosorbent 659 assay for the detection of the organophosphorus insecti- cide acephate. J Agric Food Chem 51:3695–3703 Matsumoto T, Fukuda H, Ueda M, Tanaka A, Kondo A (2002) Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p ﬂocculation functional domain. Appl Environ Microbiol 68:4517–4522 Ranina EI, Efremenco EN, Varfolomeyev SD, Simonian AL, Wild JR (1996) The development of a new biosensor based on recombinant E. coli for the direct detection of organophosphorus neurotoxins. Biosens Bioelectron 11:991–1000 Shi H, Su WW (2001) Display of green ﬂuorescent protein on Escherichia coli cell surface. Enzym Microb Technol 28: 25–34 Shimazu M, Mulchandani A, Chen W (2001) Cell surface display of organophosphorus hydrolase using ice nucle- ation protein. Biotechnol Prog 17:76–80 Tajima M, Nogi Y, Fukasawa T (1985) Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast 1:67–77 Takayama K, Suye S, Kuroda K, Ueda M, Kitaguchi T, Tsuchiyama K, Fukuda T, Chen W, Mulchandani A (2006) Surface display of organophosphorus hydrolase on Saccharomyces cerevisiae. Biotechnol Prog 22:939–943 Tuovinen K, Kalistekorhonen E, Raushel FM, Ha¨nninen O (1994) Phosphotriesterase promising candidate for use in detoxiﬁcation of organophosphates. Fundam Appl Toxicol 23:578–584 123