PAGE 9 OF 9 Nucleic Acids Research, 2009, Vol. 37, No. 18 e122 28. Fogg,M.J., Pearl,L.H. and Connolly,B.A. (2002) Structural basis for uracil recognition by archaeal family B DNA polymerases. Nat. Struct. Biol., 9, 922–927. 29. Hogrefe,H.H., Hansen,C.J., Scott,B.R. and Nielson,K.B. (2002) Archaeal dUTPase enhances PCR ampliﬁcations with archaeal DNA polymerases by preventing dUTP incorporation. Proc. Natl Acad. Sci. USA, 99, 596–601. 30. Keppler,A., Gendreizig,S., Gronemeyer,T., Pick,H., Vogel,H. and Johnsson,K. (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat. Biotechnol., 21, 86–89. 31. Hofacker,I.L. (2003) Vienna RNA secondary structure server. Nucleic Acids Res., 31, 3429–3431. 32. SantaLucia,J. Jr. (1998) A uniﬁed view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc. Natl Acad. Sci. USA, 95, 1460–1465. 33. Sambrook,J. and Russel,D.W. (2001) Molecular Cloning—A Laboratory Manual, Vol. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 8.37–38.41. 34. Wetmur,J.G. (1991) DNA probes: applications of the principles of nucleic acid hybridization. Crit. Rev. Biochem. Mol. Biol., 26, 227–259. 35. Young,B.D. and Anderson,M.L.M. (1985) In Hames,B.D. and Higgins,S.J. (eds), Nucleic Acid Hybridization: A Practical Approach. IRL Press, Oxford, pp. 47–71. 36. Georgescu,R., Bandara,G. and Sun,L. (2003) In Arnold,F.H. and Georgiou,G. (eds), Directed Evolution Library Creation—Methods and Protocols, Vol. 231. Humana Press, Totowa, NJ, pp. 75–83. 37. Sambrook,J. and Russel,D.W. (2001) Molecular Cloning—A Laboratory Manual, Vol. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 13.36–13.39. 38. Roche-Applied-Science. (2001) Manual for RTS E. coli, Linear Template Generation Set, Mannheim. 39. Shevchuk,N.A., Bryksin,A.V., Nusinovich,Y.A., Cabello,F.C., Sutherland,M. and Ladisch,S. (2004) Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously. Nucleic Acids Res., 32, e19.