1998 Nucleic Acids Research, 2005, Vol. 33, No. 6 noteworthy that 24 of 40 clones for 72 h.p.i. and also 13 out of 16 clones for 48 h.p.i. had the identical sequence (TG or TGT, shown within parentheses in Figure 3B) at the 30 end of the RNA template region (i.e. the start site of reverse transcription). The 50-UG-30 or 50-UGU-30 sequences are presumably base-paired with the bottom strand of TSD, 50-AGTAGATAGGGACA-30, suggesting the interaction between the template RNA and the target 28S rDNA sequence