3410 Nucleic Acids Research, 2011, Vol. 39, No. 8 Figure 4. Dissection of RNA proﬁles of the mutants in native condition. Phosphorimage of RNA incubated at 35 C was taken, its signal intensity distribution analyzed and represented by the line chart. (A) Raw RNA proﬁles from northern blot. (B) Mobility of peak signals of monomeric RNA (Step0 and PR) or dimeric RNA (others). (C) The RNA proﬁle transition from immature ( PR or Step0) to mature (WT) conditions. The proﬁle of WT was drawn as shaded charts in each graph. Signal intensities were measured at every 0.01 mm and total value of each signal was set to one. The plotted data are the value at every 0.5 mm. The data are the average of three separate experiments, and the error bars represent SEM.