5250 Nucleic Acids Research, 2010, Vol. 38, No. 15 Figure 6. PR gene expression can be silenced by agRNAs containing LNA. (A) Duplex sequences: passenger strands are listed on top in all cases; for siRNA PR2526 this is the sense strand, but for agRNA PR-9 this is the antisense strand. LNA sequences contain 5-methylcytosine instead of cytosine. Modiﬁcation codes: dna, RNA, lna. (B) PR-9 analogs and controls containing four evenly distributed LNA units. (C) PR-9 analogs containing LNA at strand ends. (D) PR2526 analogs containing LNA at strand ends. All data are normalized to mismatch control MM4. RNA levels are the average (±SD) from at least two independent transfections. *P < 0.05, **P < 0.01, ***P < 0.005. Protein levels were also conﬁrmed by at least two independent transfections, from which a typical western blot is shown.