962 Nucleic Acids Research, 2006, Vol. 34, No. 3 Figure 3. General physicochemical properties of activators. Mean and SEM of physicochemical properties is shown for several protein sets. (A) Isoelectric point (pI), (B) molecular mass (Mr), (C) GRAVY score (overall hydrophobicity), (D) aromaticity and (E) Codon adaptation index (CAI). For details see text. directly with the puriﬁed SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts (16). A physical interaction of YFL049W with the SWI/SNF complex together with the strong transcription activation properties (strong Y2H activator) strongly supports the possible transcription stimulation function involving the SWI/SNF complex. YJR070C is both localized to the nucleus and the cyto- plasm. Nucleic acid binding proteins are overrepresented in its set of genetic and physical interaction partners. As it is known to bind eIF5 (49) it might have regulatory functions involving translation in the cytoplasm and transcription in the nucleus.