Appl Microbiol Biotechnol (2010) 85:459–470 Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono- Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin-i T, Abe H, Shimada T, Morishita S, Sasaki T (2004) The genome sequence of silkworm, Bombyx mori. DNA Res 11:27–35 Miyajima A, Schreurs J, Otsu K, Kondo A, Arai K, Maeda S (1987) Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level of expression and secretion of biologically active mouse inteleukin-3. Gene 58:273–281 Mori H, Nakazawa H, Shirai N, Shibata N, Sumida M, Matsubara F (1992) Foreign gene expression by a baculovirus vector with an expanded host range. J Gen Virol 73:1877–1880 Motohashi T, Shimojima T, Fukagawa T, Maenaka K, Park EY (2005) Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. Biochem Biophys Res Commun 326:564–569 Muneta Y, Nagaya H, Minagawa Yu, Enomoto C, Matsumoto S, Mori Y (2004) Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworm using a hybrid baculovirus expression system. Biotechnol Lett 26:1453–1458 Nagaya H, Kanaya T, Kaki H, Tobita Y, Takahashi M, Takahashi H, Yokomizo Y, Inumaru S (2004) Establishment of a large-scale purification procedure for purified recombinant bovine interferon-τ produced by a silkworm-baculovirus gene expres- sion system. J Vet Med Sci 66:1395–1401 Nakajima M, Kato T, Kanamasa S, Park EY (2009) Molecular chaperone- assisted production of human α-1, 4-N-acetylglucosaminyltransferase in silkworm larvae using recombinant BmNPV bacmid. Mol Biotechnol 43:67–75 Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, RV Bruggner, J Costas, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O’leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW (2007) Genome sequence of Aedes aegypti, a major arbovirus vector. Science 318:1718–1723 Ogata M, Murata T, Murakami K, Suzuki T, I-PJ HK, Suzuki Y, Usui T (2007) Chemoenzymatic synthesis of artificial glycopolypep- tides containing multivalent sialyl oligosaccharides with a γ- polyglutamic acid backbone and their effect on inhibition of infection by influenza viruses. Bioorg Med Chem 15:1383– 1393 Ogata M, I-PJ HK, Murata T, Shimada S, Kozaki W, Park EY, Suzuki T, Usui T (2009a) Chemoenzymatic synthesis of sialoglycopolypeptides as glycomimetics to block infection by avian and human influenza viruses. Bioconjugate Chem 20:539–549 Ogata M, Nakajima M, Kato T, Obara T, Yagi H, Kato K, Usui T, Park EY (2009b) Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat α2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae. BMC Biotechnol 9:54 469 Palhan VB, Sumathy S, Gopinathan KP (1995) Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. Biotechniques 19(100):102–104 Park EY, Kageshima A, Kwon MS, Kato T (2007) Enhanced production of secretory Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence. J Biotechnol 129:681–688 Park EY, Abe T, Kato T (2008) Improved expression of fusion protein using cysteine-protease-and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Biotechnol Appl Biochem 49:135–140 Park EY, Ishikiriyama M, Nishina T, Kato T, Yagi H, Kato K, Ueda H (2009) Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmid and its N-linked glycan structure. J Biotechnol 139:108–114 Pérez-Filgueira DM, Resino-Talaván P, Cubillos C, Angulo I, Barderas MG, Barcena J, Escribano JM (2007) Development of a low-cost, insect larvae-derived recombinant subunit vaccine against RHDV. Virol 364:422–430 Qiu P, Ding Y, Qin J, Han KK, Zhu D (1994) Expression of biologically active monomeric form of human M-CSF in baculovirus infected silkworm, Bombyx mori. Biol Chem Hoppe Seyler 375:413–418 Sarramegna V, Talmont F, Demange P, Milon A (2003) Heterologous expression of G-protein-coupled receptors: comparison of ex- pression systems from the standpoint of large-scale production and purification. Cell Mol Life Sci 60:1529–1546 Sasaki K, Kajikawa M, Kuroki K, Motohashi T, Shimojima T, Park EY, Kondo S, Yagi H, Kato K, Maenaka K (2009) Silkworm expression and sugar profiling of human immune cell surface receptor, KIR2DL1. Biochem Biophys Res Commun 387:575– 380 Schengrund C-L (2003) “Multivalent” saccharides: development of new approaches for inhibiting the effects of glycosphingolipid- binding pathogens. Biochem Pharmacol 65:699–707 Shivappa RB, McAllister PE, Edwards GH, Santi N, Evensen Ø, Vakharia VN (2005) Development of a subunit vaccine for infectious pancreatic necrosis virus using a baculovirus insect/ larvae system. Dev Biol Basel Karger 121:165–174 Sugiura T, Sugita S, Imagawa H, Kanaya T, Ishiyama S, Saeki N, Uchiyama A, Tanigawa M, Kuwano A (2001) Serological diagnosis of equine influenza using the hemagglutinin protein produced in a baculovirus expression system. J Virol Methods 98:1–8 Suzuki Y, Nagano Y, Kato H, Matsumoto M, Nerome K, Nakajima K, Nobusawa E (1986) Human influenza A virus hemagglutinin distinguishes sialyl oligosaccharides in membrane-associated gangliosides as its receptor which mediates the adsorption and fusion processes of virus infection. J Biol Chem 261:17057– 17061 Suzuki T, Kanaya T, Okazaki H, Ogawa K, Usami A, Watanabe H, Kadono-Okuda K, Yamakawa M, Sato H, Mori H, Takahashi S, Oda K (1997) Efficient protein production using a Bombyx mori nuclear polyhedrosis virus lacking the cysteine proteinase gene. J Gen Virol 78:3073–3080 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Lett 520:97–101 Tamura T, Thibert C, Royer C, Kanda T, Abraham E, Kamba M, Komoto N, Thomas JL, Mauchamp B, Chavancy G, Shirk P, Fraser M, Prudhomme JC, Couble P (2000) Germ line transformation of the silkworm Bombyx mori L. using piggyBac transposon-derived vector. Nat Biotechnol 18:81–84 Tateno M, Toyooka M, Shikano Y, Takeda S, Kuwabara N, Sezutsu H, Tamura T (2009) Production and characterization of the recombinant human μ-opioid receptor from transgenic silk- worms. J Biochem 145:37–42