Nucleic Acids Research, 2011, Vol. 39, No. 10 4115 Figure 3. Identiﬁcation of the 42 kDa polypeptides as the hnRNPG homologues by Mass spectrometry analysis. (A) MALDI-TOF MS spectrum of one of the 42 kDa spots. The peaks are labelled with stars when the mass was identiﬁed as an hnRNP G peptide. (B) The peak at 1435.7 m/z was fragmented for further analysis. The N-to-C sequence of this peptide could be obtained directly by reading the spectrum from right to left (arrow). Thus, the sequence was 10LFIGGLNTETNEK22 and corresponded to an hnRNP G-speciﬁc peptide. Some a-, b-type and immonium fragments were also present and conﬁrmed this unambiguous identiﬁcation.