Table 2. Strong transcriptional activators when fused C-terminally to a Gal4–DBD and expressed in yeast strain with Gal4-binding sites in the His3 reporter gene Name ACE2 ACF2 ADR1 AOS1 APC4 APL2 ASM4 ATC1 BCK2 BFR2 CAK1 CHA4 CRZ1 CSE2 CUP2 ECM22 FCP1 GAC1 GAL11 GLN3 GYP1 HAP4 HSF1 IDS2 IKI1 IME1 ISF1 LTV1 MAS6 MBR1 MCD4 MED4 MED8 MET4 MRS6 MSB4 MSN2 MVP1 NAM7 NAP1 NCA3 NUP100 PAT1 PCL6 PDC2 PEX19 PFK27 PHO4 PPG1 PUP2 PUT3 REC114 RIM15 RLM1 ROX3 RPA12 RPB3 RPI1 RPP1A RSP5 RTG1 SCD5 SCH9 SIN4 SIW14 SLA1 SNF2 SPT21 ORF YLR131C YLR144C YDR216W YPR180W YDR118W YKL135C YDL088C YDR184C YER167W YDR299W YFL029C YLR098C YNL027W YNR010W YGL166W YLR228C YMR277W YOR178C YOL051W YER040W YOR070C YKL109W YGL073W YJL146W YHR187W YJR094C YMR081C YKL143W YNR017W YKL093W YKL165C YOR174W YBR193C YNL103W YOR370C YOL112W YMR037C YMR004W YMR080C YKR048C YJL116C YKL068W YCR077C YER059W YDR081C YDL065C YOL136C YFR034C YNR032W YGR253C YKL015W YMR133W YFL033C YPL089C YBL093C YJR063W YIL021W YIL119C YDL081C YER125W YOL067C YOR329C YHR205W YNL236W YNL032W YBL007C YOR290C YMR179W Process/function Transcription Cytoskeleton Transcription Protein modification Protein catabolism Vesicular transport Nuclear organization Response to stress# Cell cycle# Vesicular transport# Meiosis,cell cycle Transcription Transcription Transcription Transcription Transcription Transcription Meiosis Transcription Transcription Vesicular transport Transcription Transcription Meiosis# Transcription Transcription Cellular respiration# Unknown# Transport Cellular respiration# Protein modification# Transcription Transcription Transcription Transport Cytoskeleton Response to stress Transport# RNA metabolism Budding Organelle organization# Nuclear organization Cell cycle# Carbohydrate metabolism Transcription Organelle organization# Carbohydrate metabolism Transcription Protein modification Response to stress Transcription DNA metabolism# Response to stress Cell wall Transcription Transcription Transcription Signal transduction Protein biosynthesis Nuclear organization Transcription Vesicular transport Protein modification Transcription Response to stress Cell wall Transcription Transcription# Loc. N S N N N C N,ER N C,N N C N C,N N N N N N N G N N C,N N N ? C M ? S N N N C S N C C C,N ? N,ER C N C C C,N C,N N,ER N N C N N N N N C N C,N V C N C S N N Nucleic Acids Research, 2006, Vol. 34, No. 3 959 Table 2. Continued Name SSN2 STE5 SWI3 SWI5 TOA1 UBP3 YAL014C YAP1802 YBL081W YBR061C YBR271W YCR082W YDL161w YDR031w YDR213W YDR223W YDR260C YDR291W YDR320C YDR330W YDR489W YDR520C YER045c YER051W YGL036W YGL079W YGL223C YGR120C YGR130C YHL012W YHR160C YIL019W YIL079C YJL029C YJL181W YJR070C YJR119C YKL059C YKL171W YKR060W YKR064W YKR077W YLL013C YLR135W YLR192C YLR285W YLR445W YML037C YMR030W YMR048W YMR181C YMR195W YMR295C YMR299C YNR069C YOR066W YOR166C YOR262W YPL105C YPL229W YPL233W YPL250C YPR008W YPR179C ORF YDR443C YDR103W YJL176C YDR146C YOR194C YER151C YAL014C YGR241C YBL081W YBR061C YBR271W YCR082W YDL161W YDR031W YDR213W YDR223W YDR260C YDR291W YDR320C YDR330W YDR489W YDR520C YER045C YER051W YGL036W YGL079W YGL223C YGR120C YGR130C YHL012W YHR160C YIL019W YIL079C YJL029C YJL181W YJR070C YJR119C YKL059C YKL171W YKR060W YKR064W YKR077W YLL013C YLR135W YLR192C YLR285W YLR445W YML037C YMR030W YMR048W YMR181C YMR195W YMR295C YMR299C YNR069C YOR066W YOR166C YOR262W YPL105C YPL229W YPL233W YPL250C YPR008W YPR179C Process/function Transcription Signal transduction Transcription Transcription Transcription Protein modification Transport Vesicular transport Unknown# Protein biosynthesis Unknown Unknown# Vesicular transport Unknown# Transcription Unknown# Cell cycle# Unknown Organelle organization# Protein catabolism# DNA metabolism Unknown# Transcription Unknown# Unknown# Unknown# Vesicular transport# Vesicular transport Unknown# Unknown# Transport Unknown# Transport# Vesicular transport# Unknown# Unknown Unknown# RNA metabolism# Protein catabolism Ribosome biogenesis Unknown# Unknown# RNA metabolism DNA metabolism Protein biosynthesis Vitamin metabolism Unknown# Unknown# Cellular respiration# Cell cycle# Unknown# Unknown# Unknown# Unknown# Unknown# Unknown# Unknown# Unknown# Unknown# Unknown# Cell cycle# Unknown# Transcription Transcription Loc. N V,C,N N C,N N C C S ? C C C,N S C,N C,N ? N N ER C,N N C,N N ? C C G G C ? P C,N N G,C ? C,N C,N N C N C,N C,N C N C C ? V M,N N ? V S C ? ? ? C C C N ? N N 3AT concentrations of 200 mM or more are required to suppress the amount of His3 expressed in these strains. Process/function abbreviated after GO annota- tion in SGD, # ¼ molecular function unknown. Localization (Loc.) encoded as nuclear (N), cytoplasmic (C), cytoskeletal/cell wall (S), Golgi (G), endoplasmic reticulum (ER), peroxisome (P), vesicle/membranes (V), mitochondrion (M) or unknown (?).