1094 Appl Microbiol Biotechnol (2009) 84:1087–1094 Van der Linden RH, Frenken LG, De Geus B, Harmsen MM, Ruuls RC, Stok W, de Ron L, Wilson S et al (1999) Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochim Biophys Acta 1431:37–46 Van der Vaart JM (2002) Expression of VHH antibody fragments in Saccharomyces cerevisiae. In: O'Brien PM, Aitken R (eds) Methods in molecular biology, Vol. 178, antibody phage display: methods and protocols. Humana, Totowa, NJ, pp 359–366 Wright A, Morrison SL (1994) Effect of altered CH2-associated carbohydrate structure on the functional properties and in vivo fate of chimeric mouse-human immunoglobulin G1. J Exp Med 180:1087–1096