470 Tomita M, Munetsuna H, Sato T, Adachi T, Hino R, Hayashi M, Shimizu NN, Tamura T, Yoshizato K (2003) Transgenic silk- worms produce recombinant human type III procollagen in cocoons. Nat Biotechnol 21:52–56 Wang Y, Wu X, Liu G, Cao C, Huang H, Xu Z, Liu J (2005) Expression and porcine lactoferrin by using recombinant bacu- lovirus in silkworm, Bombyx mori L., and its purification and characterization. Appl Microbiol Biotechnol 69:385–389 Wei WL, Qin JC, Sun MJ (2000) High-level expression of human butyrylcholinesterase gene in Bombyx mori and biochemical– pharmacological characteristic study of its product. Biochem Pharmacol 60:121–126 Wess J (1997) G-protein-coupled receptors: molecular mechanisms involved in receptor activation and selectivity of G-protein recognition. FASEB J 11:346–354 Wise A, Jupe SC, Rees S (2004) The identification of ligands at orphan G-protein coupled receptors. Annu Rev Pharmacol Toxicol 44:43–66 Xia Q, Zhou Z, Lu C, Cheng T, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, Wan Y, Zhu Y, Yu M, Shen W, Wu D, Xiang Z, Yu J, Wang J, Li R, Shi J, Li H, Li G, Su J, Wang X, Li G, Zhang Z, Wu Q, Li J, Zhang Q, Wei N, Xu J, Sun H, Dong L, Liu D, Zhao S, Zhao X, Meng Q, Lan F, Huang X, Li Y, Fang L, Li C, Li D, Sun Y, Zhang Z, Yang Z, Huang Y, Xi Y, Qi Q, He D, Huang H, Zhang X, Wang Z, Li W, Cao Y, Yu Y, Yu H, Li J, Ye J, Chen H, Zhou Y, Liu B, Wang J, Ye J, Ji H, Li S, Ni P, Zhang J, Zhang Y, Zheng H, Mao B, Wang W, Ye C, Li S, Wang J, Wong GKS, Yang H (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306:1937–1940 Appl Microbiol Biotechnol (2010) 85:459–470 Xia Q, Cheng D, Duan J, Wang G, Cheng T, Zha X, Liu C, Zhao P, Dai F, Zhang Z, He N, Zhang L, Xiang Z (2007) Microarray- based gene expression profile in multiple tissue of the domesti- cated silkworm Bombyx mori. Genome Biol 8:R162 Yamada S, Suzuki Y, Suzuki T, Li MQ, Nidom CA, Sakai-Tagawa Y, Muramoto Y, Ito M, Kiso M, Hiromoto T, Shinya K, Sawada T, Kiso M, Usui T, Murata T, Lin Y, Hay A, Haire LF, Stevens DJ, Russell RJ, Gamblin SJ, Skehel JJ, Kawaoka Y (2006) Hemagglutinin mutations responsible for the binding of H5N1 influenza A viruses to human-type receptors. Nature 444:378–382 Yamamoto M, Yamao M, Nishiyama H, Sugihara S, Nagaoka S, Tomita M, Yoshizato K, Tamura T, Mori H (2004) New and highly efficient method for silkworm transgenesis using Auto- grapha californica nucleopolyhedrovirus and piggyBac transpos- able elements. Biotechnol Bioeng 88:849–853 Yamao M, Katayama N, Nakazawa H, Yamakawa M, Hayashi Y, Hara S, Kamei K, Mori H (1999) Gene targeting in the silkworm by use of a baculovirus. Genes Dev 13:511–516 Yue W, Miao Y, Li X, Wu X, Zhao A, Nakagaki M (2006) Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV baculovirus expression system. Appl Microbiol Biotechnol 73:181–186 Zhang Y, Hu J, Miao Y, Zhao A, Zhao T, Wu D, Liang L, Miikura A, Shiomi K, Kajiura Z, Nakagaki M (2008) Expression of EGFP- spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline protein yield. Mol Biol Rep 35:329–335 Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu Wei, Jiang C, Liu, L, Sheng Q, Jin Y, Wu X (2009) Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Inter Genomics. doi:10.1007/s10142-009-0127-x