Satyal SH, Schmidt E, Kitagawa K, Sondheimer N, Lindquist S, Kramer MJ, Mori- moto RI (2000) Polyglutamine aggregates alter protein folding homeostasis in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U S A 97, 5750–5755. Strange K, Christensen M, Morrison R (2007) Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies. Nat. Protoc. 2, 1003–1012. Walker GA, Lithgow GJ (2003) Lifespan extension in C. elegans by a molec- ular chaperone dependent upon insulin-like signals. Aging Cell 2, 131– 139. Walker GA, White TM, McColl G, Jenkins NL, Babich S, Candido PM, Johnson TE, Lithgow GJ (2001) Heat shock protein accumulation is upregulated in a long-lived mutant of Caenorhabditis elegans. J. Gerontol. A Biol. Sci. Med. Sci. 56, B281–B287. Wang HD, Kazemi-Esfarjani P, Benzer S (2004) Multiple-stress analysis for isola- tion of Drosophila longevity genes. Proc. Natl. Acad. Sci. U S A 101, 12610– 12615. Yokoyama K, Fukumoto K, Murakami T, Harada S, Hosono R, Wadhwa R, Mitsui Y, Ohkuma S (2002) Extended longevity of Caenorhabditis elegans by knock- ing in extra copies of hsp70F, a homolog of mot-2 (morta- lin) ⁄ mthsp70 ⁄ Grp75. FEBS Lett. 516, 53–57. Zid BM, Rogers AN, Katewa SD, Vargas MA, Kolipinski MC, Lu TA, Benzer S, Kapahi P (2009) 4E-BP extends lifespan upon dietary restriction by enhancing mitochondrial activity in Drosophila. Cell 139, 149–160. Aging, protein solubility and lifespan in C. elegans, P. Reis-Rodrigues et al. 127 Supporting Information Additional supporting information may be found in the online version of this article: Fig. S1 DAF-21 and ACO-2 accumulate in the insoluble fraction of aged C. elegans. Table S1 List of insoluble proteins in aged C. elegans. Table S2 Survival analysis knocking down transcripts encoding insoluble proteins Table S3 Survival analysis of a randomly generated list of genes encoding proteins identiﬁed in a total lysate As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing ﬁles) should be addressed to the authors. Published 2011. This article is a U.S. Government work and is in the public domain in the USA. Aging Cell ª 2011 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland