A C B D Cultivated limbal stem cell transplantation Figure 8 A. Slit lamp photograph of a patient with total limbal stem cell deﬁciency following alkali injury. B. Slit lamp photograph of the cornea showing a persistent scar with stable ocular surface, a year after cultivated LSCT. C. Slit lamp photograph at 6 months after penetrating keratoplasty. D. Photomicrograph of the hematoxylin and eosin-stained section of the excised corneal button showing stratiﬁed corneal epithelium subsequent to cultivated LSCT. Disclosure The authors have no proprietary interests in the materials or methods of this article. References Anderson DF, Ellies P, Pires RT, et al. 2001. Amniotic membrane in partial limbal stem cell deﬁciency. Br J Ophthalmol, 85:567–75. Coster DL, Aggarwal RK, Williams KA. 1995. Surgical management of ocular surface disorders using conjunctival and stem cells allografts. Br J Ophthalmol, 79:977–82. Cotsarelis G, Cheng SZ, Dong G, et al. 1989. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cells, 57:201–9. Daya SM, Bell RWD, Habib NE, et al. 1999. Clinical and pathological ﬁnd- ings in keratolimbal allograft rejection. Cornea, 19:443–50. Dua HS, Gomes JAP, Singh A. 1994. Corneal epithelial wound healing. Br J Ophthalmol, 78:401–8. Dua HS. 1995. Stem cells of the ocular surface: Scientiﬁc principles and clinical applications. Br J Ophthalmol, 79:968–69. Dua HS, Saini JS, Azuara-Blanco A, et al. 2000. Limbal stem cell deﬁciency: Concept, aetiology, clinical presentation, diagnosis and management. Ind J Ophthalmol, 48(2):83–92. Dua HS, Blanco AA. 1999. Allolimbal transplantation in patients with limbal stem cell deﬁciency. Br J Opthalmol, 83:414–19. Clinical Ophthalmology 2008:2(3) Espana EM, Ti SE, Grueterich M, et al. 2003. Corneal stromal changes following reconstruction by ex vivo expanded limbal epithelial cells in rabbits with total limbal limbal stem cell deﬁciency. Br J Ophthalmol, 87:1509–14. Grueterich M, Tseng SC. 2002a. Human limbal progenitor cells expanded on intact amniotic membrane ex vivo. Arch Ophthalmol, 120:783–90. Grueterich M, Espana E, Tseng SC. 2002b. Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol Vis Sci, 43:63–71. Grueterich M, Espana E, Touhami A, et al. 2002c. Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deﬁciency. Ophthalmol- ogy, 109:1547–52. Grueterich M, Espana EM, Tseng SC. 2003. Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche. Surv Ophthalmol, 48:631–46. Kenyon KR, Bulusoglu G, Ziske JD. 1990. Clinical pathologic correlations of limbal autograft transplantation. Am J Ophthalmol, 31:l–12. Kenyon KR, Tseng SCG. 1989. Limbal autograft transplantation for ocular surface disorders. Ophthalmology, 96:709–23. Kim JC, Tseng SCG. 1995. Transplantation of preserved human amniotic membrane for ocular surface reconstruction in severely damaged rabbit corneas. Cornea, 14:473–84. Koizumi N, Inatomi T, Quantock AJ, et al. 2000a. Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits. Cornea, 19:65–71. 501