J Am Oil Chem Soc (2012) 89:389–400 Fig. 3 HPLC-FLD chromatogram of a hemp seed oil fraction. Upper line shows the oil sample chromatogram, and the lower one chromatogram of the standard solution. (1 Naf, 2,3 Acy and Ace, 4 Fluo, 5 Phe, 6 Ant, 7 Flua, 8 Pyr, 9 B[a]A, 10 Chry, 11 B[b]F, 12 B[k]F, 13 B[a]P, 14 [DB[ah]A, 15 B[ghi]P, 16 IndP.) Typical chromatogram showing marker-PCB fraction extracted from an oil sample is shown in Fig. 4. Average proﬁle of the PCB fraction within the oil samples is shown in Fig. 5. PCB TEQ values determined for the studied samples are shown in Table 8. PCBs 105, 114, 118 and 126 had the highest average contribution to PCB TEQ. Average total contribution of those four congeners accounted to more that 80% of the total PCB TEQ. TEQ results calculated for 2005 TEFs were generally lower than those calculated for 1998 TEFs. This is inﬂuenced mainly by decreased TEF values for mono-ortho-PCB in the 2005 TEF scheme. Majority of the investigated PBDE congeners in the oil samples were below LOQ. BDE 47, 77 and 99 were the most abundant and subsequently the most frequently congeners found. The highest total concentration of 14 PBDEs was (19.3 pg g-1) found in the linaire oil sample. PCBs and PBDEs in oil may be attributable mainly to environmental contamination. However, such contaminants are typical for high-fat food products of animal origin Borage 14935.3 9591.3 335.4 11.4 5.6 3.3 24882.3 19.0 0.7 28.6 23.9 54.3 1.4 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ 123 397 Evening star 624.7 377.8 29.7 16.1 7.5 4.2 1060.0 2.8 \LOQ 6.1 3.5 13.6 0.3 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ Table 6 Average contents of the studied PCB congeners in the analyzed oil samples (mean value of two parallel determinations for the composite oil sample) PCB Congener Concentration (pg g-1) Poppy seed Rapeseed Sesame seed Pumpkinseed Hempseed Linaire 28 52 101 138 153 180 P6 77 81 105 114 118 123 126 156 157 167 169 189 135.3 104.7 29.4 33.4 9.9 6.6 319.2 2.0 \LOQ 8.6 1.1 14.2 0.2 \LOQ 1.0 \LOQ \LOQ 0.2 \LOQ 229.6 187.8 16.7 4.6 2.9 2.3 443.9 1.8 \LOQ 4.2 2.0 7.2 0.2 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ 787.6 664.2 85.7 8.4 5.0 1.8 1552.7 8.4 \LOQ 21.3 2.2 33.0 1.0 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ 59.2 25.9 9.9 21.1 6.4 4.6 127.2 1.0 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ 125.7 72.3 15.9 10.8 6.2 2.9 233.7 \LOQ 0.7 2.4 1.8 4.8 0.0 \LOQ \LOQ \LOQ \LOQ \LOQ \LOQ 127.3 56.4 18.5 27.6 9.3 10.8 250.0 1.0 \LOQ 2.9 2.9 6.2 0.0 \LOQ 1.9 \LOQ 0.7 \LOQ \LOQ