CHF1/Hey2 Regulates Hypertrophy transport may be important for cardiac hypertrophy (Fig. (3)). In addition, aquaporins 3, 7 and 9 also transport glyc- erol and are known as aquaglyceroporins. AQ7 mice develop adipocyte hypertrophy, obesity and diabetes due to impaired glycerol efflux [20-22]. These findings raise the intriguing possibility that suppression of glycerol efflux in cardiac myocytes may prevent the onset of pathological hy- pertrophy, perhaps through supporting fatty acid metabolism. Genes involved in regulation of adenylate cyclase signaling (GO:0045761, Fig. (4)) include multiple G protein coupled Current Genomics, 2010, Vol. 11, No. 4 291 receptors such as ADCYAP1R1, LTB4R2, ADRB2, GABBR1, GALR3, GHRHR, CRHR1, ADRA2A, VIPR2, GRM4 and intracellular regulators of the adenylate cyclase response such as ADCY4, GNAL, ADCY2, ADCY7, ADCY1. Given the known role of adrenergic signaling in the pathogenesis of cardiac hypertrophy and heart failure (re- viewed in [23]), suppression of adenylate cyclase activity is consistent with these findings. Fig. (3). Heat map for genes involved in water transport (GO: 0006833). Columns represent microarray data obtained from inde- pendent primary cultures of cells of the indicated genotype and therefore represent biological replicates. Gene name abbreviations for each component of the gene set are indicated at the end of each row. Green indicates expression is lower, while red indicates ex- pression is higher than average of WT. The genes in this set are predominantly downregulated in the transgenic cells (TgS1/2/3/4/5/ 6/7) in the presence of serum relative to the WT cells treated with serum (WTS1/2/3/4/5/6/7). Fig. (4). Heat map for genes related to regulation of adenylate cy- clase activity (GO: 0045761). Columns represent microarray data obtained from independent primary cultures of cells of the indicated genotype and therefore represent biological replicates. Gene name abbreviations for each component of the gene set are indicated at the end of each row. Green indicates expression is lower, while red indicates expression is higher than average of WT. The genes in this set are predominantly downregulated in the transgenic cells (TgS1/2/3/4/5/6/7) in the presence of serum relative to the WT cells treated with serum (WTS1/2/3/4/5/6/7).