Strain C. crescentus CB15N CJW932 CJW1231 CJW1233 CJW1474 CJW1475 CJW1478 CJW1518 CJW1520 CJW1521 CJW1522 CJW1535 CJW1536 CJW2862 CJW2927 LS3812 E. coli DH5a S17-1 BL21 (DE3) CJW1659 CJW1353 CJW2054 CJW2045 CJW3663 Plasmid pBluescriptKSþ pET29a pET29acreS pET29acreSDN27 pET29acreSDL1 pET29acreSSAT pET29acreSDT pHL23 pMR20 pMR20creS-tc pMR20creSDN28-79-tc pMR20creSDL1-tc pMR20creSDR-tc pMR20creSDT pMR20creSSAT-tc n 208 Cabeen et al. Table I. Strains and Plasmids Used in This Work Relevant genotype or description Synchronizable variant strain of CB15; also known as NA1000 CB15N creS::pJM21creS-ﬂag CB15N/pMR20creS-tc CB15N DcreS/pMR20creS-tc CB15N/pMR20creSDT CB15N DcreS/pMR20creSDT CB15N/pMR20creSDR-tc CB15N DcreS/pMR20creSSAT-tc CB15N/pMR20creSDL1-tc CB15N DcreS/pMR20creSDL1-tc CB15N/pMR20creSSAT-tc CB15N/pMR20creSDN28-79-tc CB15N DcreS/pMR20creSDN28-79-tc CB15N DcreS/pMR20creSDR-tc CB15N DcreS/pMR20creSDN27-tc CB15N DcreS Cloning strain RP4-2 Tc::Mu KM-Tn7, for plasmid mobilization F_ompT hsdSB (rB_ mB_) dcm gal (DE3) BL21 (DE3)/pET29acreS BL21 (DE3)/pET29acreSDN27 BL21 (DE3)/pET29acreSDL1 BL21 (DE3)/pET29acreSSAT BL21 (DE3)/pET29acreSDT Description AmpR cloning vector Protein expression plasmid pET29a carrying creS coding sequence pET29a carrying creSDN27 coding sequence pET29a carrying creSDL1 coding sequence pET29a carrying creSSAT coding sequence pET29a carrying creSDT coding sequence KanR cloning vector TetR low-copy-number broad host range vector pMR20 carrying creS-tc gene, coding for crescentin with a C-terminal tetracysteine tag pMR20 carrying creS-tc gene deleted for N28-79 coding sequence pMR20 carrying creS-tc ORF deleted for linker 1 coding sequence pMR20 carrying creS-tc gene deleted for rod coding sequence pMR20 carrying creS gene deleted for tail coding sequence pMR20 carrying creS-tc gene with coding sequence for Ser-Ala-Thr inserted at stutter site Reference or source Evinger and Agabian, 1977 Ausmees et al., 2003 This study Cabeen et al., 2009 This study This study This study This study This study Cabeen et al., 2009 This study This study This study This study Cabeen et al., 2009 Gitai et al., 2004 Invitrogen Simon et al., 1983 Novagen This study This study This study This study This study Reference or Source Stratagene Novagen This study This study This study This study This study Lam et al., 2006 Roberts et al., 1996 Cabeen et al., 2009 This study Cabeen et al., 2009 This study This study This study CYTOSKELETON