Original Article Elmer ress J Clin Med Res • 2012;4(2):110-113 The Investigation of Serum Vaspin Level in Atherosclerotic Coronary Artery Disease Mehmet Ali Kobata, e, Ahmet Celika, Mehmet Balina, Yakup Altasa, Adil Baydasb, Musa Bulutb, Suleyman Aydinc, Necati Daglib, Mustafa Ferzeyn Yavuzkirb, Selcuk Ilhand Abstract Background: It was speculated that fatty tissue originated adipo- cytokines may play role in pathogenesis of atherosclerosis. These adipocytokines may alter vascular homeostasis by effecting endo- thelial cells, arterial smooth muscle cells and macrophages. Vaspin is a newly described member of adipocytokines family. We aimed to investigate whether plasma vaspin level has any predictive value in coronary artery disease (CAD). Methods: Forty patients who have at least single vessel ≥ 70 % stenosis demostrated angiographically and 40 subjects with normal coronary anatomy were included to the study. The vaspin levels were measured from serum that is obtained by centrifigation of blood and stored at -20 oC by ELISA method. The length, weight and body mass index of patients were measured. Biochemical pa- rameters including total cholesterol, low density lipoprotein, high density lipoprotein, creatinine, sodium, potassium, hemoglobine, uric acid and fasting glucose were also measured. Results: Biochemical markers levels were similar in both groups. Serum vaspin levels were significantly lower in CAD patients than Manuscript accepted for publication February 22, 2012 aDepartment of Cardiology, Elazig Education and Research Hospital, Elazig, Turkey bDepartment of Cardiology, Firat University Medical Faculty, Elazig, Turkey cDepartment of Biochemistry, Firat University Medical Faculty, Elazig, Turkey dDepartment of Pharmacology, Firat University Medical Faculty, Elazig, Turkey eCorresponding author: Mehmet Ali Kobat. Email: mehmetalikobat@yahoo.com doi:10.4021/jocmr841w control group (respectively; 256 ± 219 pg/ml vs. 472 ± 564 pg/ml, P < 0.02). Beside this serum vaspin level was lower in control group with high systolic blood pressure. Conclusions: Serum vaspin levels were found significantly lower in patients with CAD than age-matched subjects with normal coro- nary anatomy. Vaspin may be used as a predictor of CAD. Keywords: Coronary artery disease; Vaspin; Adipokine Introduction Inflammation was thought to be one of the major causes of early atherosclerosis and its’ progressive complications such as plaque rupture [1, 2]. Inflammation and blood flow are alternative pathogenic factors for plaque development [3, 4]. Recently, it was shown that adipokines including visser- al adipose tissue-derived serine protease inhibitor (VASPIN) which have endocrine and local roles in atherosclerosis de- velopment are syntesized by adipose tissue [5, 6]. Adipose tissue derived factors including adipokines has been suggest- ed as actors for premature and accelerated atherosclerosis in obese people [7]. VASPIN is a member of serine protease inhibitor family which has a regulator role in glukose and lipid metabolism [8]. The high blood vaspin concentration in obese subjects was shown [9] and it was also demonstrated in both non-obese and obese type 2 diabetic patients [10]. The relationship between VASPIN levels and coronary ar- tery disease (CAD) was unclear in the literature. In the present study we aimed to show the relationship between serum VASPIN levels and patients with angio- graphically proven coronary artery disease. Methods In this study, we screened 40 consecutive patients with stable angina pectoris who had at least 70% stenosis in any coronary artery diagnosed by coronary angiography (CAD Group) and 40 subjects with normal coronary anatomy that 110 Articles © The authors | Journal compilation © J Clin Med Res and Elmer Press™ | www.jocmr.org