Database searches are achieved by querying the appropri- ate data category. Thus queries of different sequence features (Named genes, UniProt match descriptions, InterPro domains, GO terms) or genetic data (Phenotypes, Stocks, RNAi experi- ments) involve selecting the data category from the pull- down menu of the search box and ﬁlling in a search term. All searches are case insensitive and are surrounded by wild cards. A query will return a database page if there is only one result, but more often it will return a table with multiple results. For example, for a sequence feature search, the table will contain the name of each sequence feature linked to the corresponding database page as well as its location on the genome sequence. Gene page Suppose that we want to ﬁnd information about the gene SM19 encoding eta-tubulin (21). We can type ‘sm19’ as Gene name in the Sequence box and launch a search. The search will return a table with three results, the wild-type SM19 gene and two alleles, sm19-1 and sm19-2. The gene Nucleic Acids Research, 2007, Vol. 35, Database issue D441 page obtained by clicking on SM19 is shown in Figure 1. The gene page contains three regions, a central panel, a GBrowse image and the left sidebar with links to all related data, presented by category. The central panel shows that we are looking at a Sequence Feature of Type ‘gene’. The score in this case is that provided by Genoscope’s automated annotation (14). For sequence fea- tures of type ‘match’, i.e. for UniProt matches, it is the match score. All names and synonyms are provided, and these are all targets of Gene name searches. The ‘Annotation’ section provides GO terms and their evidence codes. Clicking on the GO term brings up a page with all the genes in Parame- ciumDB associated with the term and an inset frame with the AmiGO browser page for that term (http://www.godatabase. org/). ParameciumDB GO terms (August 2006) are electroni- cally inferred from the InterPro matches. This particular case is a good example of the limits of electronically inferred annotation. Eta-tubulin, a recently discovered member of the tubulin superfamily, also has a new function. Eta-tubulin is necessary for the process of basal body duplication and is not involved in the process of microtubule-based movement, Figure 1. A ParameciumDB gene page. This is the gene page for the gene SM19.