Journal of Proteome Research furthermore, enables development of a predictive model of proteomic features that determine whether a protein is antigenic and produces antibodies that confer protection. This systems biology approach provides an empirical basis for understanding the breadth and speciﬁcity of the immune response to B. melitensis and a new framework for comparing the humoral responses against other organisms. ’ ASSOCIATED CONTENT bS Supporting Information Supplementary tables and ﬁgures. This material is available free of charge via the Internet at http://pubs.acs.org. ’ AUTHOR INFORMATION Corresponding Author *Li Liang (lliang3@uci.edu) or Philip L. Felgner (pfelgner@ uci.edu). Phone: 949-824-1407. Fax: 949-824- 0481. Notes Conﬂict of InterestP.L.F. and D.H.D. have patent applications pertaining to this work and own stock in Anitigen Discovery Inc. that has licensed the technology. X.L. is an inventor on these patent applications and is employed at Anitigen Discovery Inc. 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