Original Article J. Clin. Biochem. Nutr., 46, 205–211, May 2010 EtjJ01KOuchoC098bryeu.18Bicno3grS20N0tn1ia-on9a605,call-40iJl9yo/e08aj3Atfcp96ypbCratfniltoci.0nurle9iFcs-ra9el3eLBRioeacdahicefamlEiRsterxysetanrrcdahNJcuatptraitnSiounppresses the Postprandial Elevation of Portal, Cardiac and Peripheral Fructose Concentrations after Sucrose Ingestion in Rats Keiichiro Sugimoto1,2,*, Tetsuro Hosotani3, Takahiro Kawasaki4,5, Kazuya Nakagawa1, Shuichi Hayashi1, Yoshihisa Nakano2,3,6, Hiroshi Inui2,3 and Toshikazu Yamanouchi4 1Research and Development Center, Nagaoka Perfumery Co. Ltd., 1-3-30 Itsukaichi, Ibaraki, Osaka 567-0005, Japan 2Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan 3Center for Research and Development of Bioresources, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan 4Department of Internal Medicine, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan 5Department of Internal Medicine, Kawakita General Hospital, 1-7-3 Asagayakita, Suginami-ku, Tokyo 166-0001, Japan 6Osaka Women’s Junior College, 3-8-1 Kasugaoka, Fujiidera, Osaka 583-8558, Japan ;5234R6023aec173cc0eeivpetedd259..192.2.2000099 Received 29 September, 2009; Accepted 5 December, 2009; Published online 20 March, 2010 CopySriugmhtm©a2r0y10 OJCvBerNintake of sucrose or fructose induces adiposity. Fructose undergoes a strong Maillard reaction, which worsens diabetic complications. To determine whether Eucalyptus globulus leaf extract (ELE) suppresses the postprandial elevation of serum fructose concentra- tions (SFCs) in the portal, cardiac, and peripheral blood after sucrose ingestion, we performed gas chromatography/mass spectrometry (GC/MS) and measured SFC without any interference by contaminating glucose in the samples. Fasting Wistar rats were orally administered water (control group) or ELE (ELE group) before sucrose ingestion. Blood was collected from the portal vein, heart, and tail. The increase in the SFCs in the portal and cardiac samples 30 min after sucrose ingestion was lower in the ELE group than in the control group. The coefficient of correlation between the SFCs in the portal and cardiac samples was 0.825. The peripheral SFC in the control group progressively increased and was 146 µmol/L at 60 min. This increase was significantly lower in the ELE group. In contrast, the serum glucose concentrations in the 2 groups were similar. ELE suppressed postprandial hyperfructosemia in the portal, cardiac, and peripheral circulations. ELE may counteract glycation caused by high blood fructose concentrations induced by the consumption of fructose-containing foods or drinks. Key Words: Eucalyptus globulus, serum fructose concentration, postprandial hyperfructosemia, intestinal fructose absorption, sucrose-tolerance test *To whom correspondence should be addressed. Tel: +81-72-623-5252 Fax: +81-72-623-5251 E-mail: ksugimoto@npc-nagaoka.co.jp 205 Introduction While starch is the major component of dietary carbo- hydrate, sucrose is also ingested in large amounts because it is used in many foods as a sweetener or for other purposes.