Sadeghian et al. Control 30.00±2.550 30.400±3.647 29.400±3.050 29.200±3.701 >0.05 Sampling days Group 1 (nano-selenium) 0.122±0.006 0.133±0.039 0.187±0.032*,** 0.184±0.012*,**,*** <0.05 Table 1 The changes in mean + SD of TBARS value in each of the groups during the different days of sampling Table 2 PCV counts (means ± SD) in each of the groups during the different days of sampling Group 2 (sodium selenite) 0.125±0.007 0.146±0.118 0.192±0.047* 0.199±0.035*,*** <0.05 Control 0.124±0.007 0.135±0.012 0.145±0.027 0.141±0.018 >0.05 Sampling days 0 10 20 30 P value Group 1 (nano-selenium) Group 2 (sodium selenite) 32.00±1.225 33.00±2.345 30.400±1.140 31.800±0.837 >0.05 30.400±1.517 30.800±2.950 31.600±3.782 28.400±3.286 >0.05 304 0 10 20 30 P value *P<0.05, significant increase to day 0; **P<0.05, significant increase to day 10; ***P<0.05, significant increase to the control group TBARS were increased to 0.187±0.032 and 0.184±0.012, respectively (P<0.05), in comparison with the basal level (0.122±0.006). The statistical survey showed that there were no significant differences on the 10th day in comparison with day 0, but a significant increase was observed in the values of TBARS on the 20th day in comparison with day 0 (P= 0.002) and the 30th day compared with day 0 (P<0.001). Besides, a significant increase was observed on the 20th (P= 0.042) and 30th day (P=0.024) in comparison with the 10th day. These results show that the maximum values of TBARS occurred on day 20 and less antioxidant properties, in day 20. In group 2 (sodium selenite), TBARS value was increased slightly on the 10th day as it reached from 0.125± 0.007 (day 0) to 0.146±0.118 on the 10th day (P>0.05). An ascending trend in TBARS value occurred on the 20th and 30th day and reached to 0.192±0.047 and 0.199±0.035, respectively (P<0.05). A significant increase on the 20th day in comparison with day 0 (P=0.013) and on the 30th day in comparison with day 0 (P=0.002) was also seen. In the control group, there were no significant differences in the TBARS value on any of the sampling days, neither with the basal level (day 0) nor with each other. By comparing the means of the three groups together, it was determined that there were no significant differences in the TBARS values on the 0, 10th, and 20th day (P>0.05), while in groups 1 and 2, a significant increase in the TBARS values was seen just on the 30th day in comparison with the control group, with P=0.003 and P=0.011, respectively. Hematological Parameters The observed PCV, RBC, WBC, neutrophil, and lymphocyte counts in each group on different days of sampling are presented in Table 2, 3, 4, 5, and 6. The PCV and RBC Counts In the three groups, there were no significant differences in the PCV and RBC counts on any of the sampling days, neither with the basal level (day 0) nor with each other. Number of WBC In group 1 (nano-selenium), on the 10th and 20th day, the numbers of WBC increased to 8,640 ±879.204 and 9,020 ± 491.935, respectively (P< 0.05), in comparison with the basal level (7,460±497.996). The statistical survey showed that there was a significant increase in WBC counts on the 10th, 20th, and 30th day in comparison with day 0 with P= 0.031, P= 0.002, and P= 0.003, respectively. As Table 4 clearly shows, in group 1, the WBC counts decreased from the 20th day on, but statistically, there were no significant differences between the 20th and 30th day (P> 0.05). The statistical survey in group 2 (sodium selenite) showed that there was a significant increase on the 10th and 20th day in comparison to day 0 (the P value was 0.045 and 0.023, respectively), and unlike group 1, there was no significant difference in the 30th day in comparison to day 0. In the control group, there were no significant differences in the WBC counts on any of the sampling days, neither with the basal level (day 0) nor with each other. By comparing the means of the three groups together, it was determined that there were no significant differences in the WBC counts on day 0 (P> 0.05). The WBC counts in group 1 showed a significant increase on the 20th and 30th day in comparison with the control group (the P value was 0.001 and 0.021, respectively), while in group 2, a significant increase in the WBC counts was seen just on the 20th day in comparison with the control group (P=0.047). Table 3 Red blood cell counts (means ± SD) in each of the groups during the different days of sampling Sampling days 0 10 20 30 P value Group 1 (nano-selenium) Group 2 (sodium selenite) 11.300±0.412 11.660±0.811 10.780±0.432 11.240±0.288 >0.05 10.820±0.492 10.900 ±0.935 11.220±1.248 10.120±1.117 >0.05 Control 10.660±0.853 10.820±1.260 10.520±1.040 10.380±1.184 >0.05