Nanoparticles of Selenium with Stronger Physiological Effects 305 Table 4 White blood cell counts (means ± SD) in each of the groups during the different days of sampling Table 6 Lymphocyte counts (means ± SD) in each of the groups during the different days of sampling Sampling days Group 1 (nano-selenium) 0 10 20 30 P value 7,460±497.996 8,640±879.204* 9,020±491.935*,** 8,580±349.285*,** <0.05 Group 2 (sodium selenit) 7,420±449.444 8,380±785.493* 8,540±931.665*,** 8,100±768.115 <0.05 Control 7,440±602.495 7,560±795.613 7,420±521.536 7,500±768.115 >0.05 *P<0.05, significant increase to day 0; **P<0.05, significant increase to the control group The Neutrophil Counts Table 5 shows that the number of neutrophils in groups 1 and 2 increased in the period between days 0 and 20, but this increase was more significant in the nano-selenium group (the P value was <0.001 in each of the three groups). The statistical survey in group 1 (nano-selenium) showed that there was a significant increase in neutrophil counts on the 10th, 20th, and 30th day in comparison with day 0 (the P value was <0.001 in each of the three groups), on the 20th day in comparison with the 10th day (P= 0.005), and on the 30th day in comparison with the 10th day (P= 0.015). In group 2 (sodium selenite), there was significant increase on the 10th, 20th, and 30th days in comparison with day 0 (the P value was 0.019, <0.001, and 0.003, respectively) and on the 20th day in comparison with the 10th day (P= 0.003), but unlike group 1, there was no significant difference on the 30th day in comparison to the 10th day. In the control group, there were no significant differences in the neutrophil counts on any of the sampling days, neither with the basal level (day 0) nor with each other. By comparing the means of the three groups together, it was determined that there were no significant differences in the neutrophil counts on day 0 (P> 0.05). The neutrophil counts in group 1 showed a significant increase on the 10th day in comparison with the two groups (P=0.050) and control (P=0.049) group, on the 20th and 30th Table 5 Neutrophil counts (means ± SD) in each of the groups during the different days of sampling Sampling Group 1 days (nano-selenium) Group 2 (sodium selenite) Control 0 10 20 30 P value 33.200±1.924 32.400±1.817 34.200±2.864 43.200±3.701*,***,**** 37.800±3.701* 35.00±7.00 63.00±10.840*,**,**** 55.200±8.289*,**,**** 36.400±1.517 53.800±6.686*,**,**** 44.600±6.387*,**** 34.400±2.510 <0.05 <0.05 >0.05 *P<0.05, significant increase to day 0; **P<0.05, significant increase to day 10; ***P<0.05, significant increase to group 2 (sodium selenite); ****P<0.05, significant increase to the control group Sampling Group 1 days (nano-selenium) Group 2 (sodium selenite) Control 0 10 20 30 P value 63.800±1.924 65.400±0.894 63.800±2.588 53.600±3.507*,****,***** 59.800±2.168* 62.200±6.723 34.200±10.498*,**,***** 41.800±9.230*,**,***** 61.800±2.775 34.200±10.498*,**,***** 53.400±6.107*,***,***** 63.600±2.881 <0.05 <0.05 >0.05 *P <0.05, significant reduction to day 0; **P <0.05, significant reduction to day 10; ***P<0.05, significant increase to day 20; ****P<0.05, significant reduction to group 2 (sodium selenite); *****P<0.05, significant reduction to the control group day compared with control group (the P value was <0.001 and <0.001, respectively), and in group 2 on the 20th and 30th day (the P value was 0.001 and 0.010, respectively) in comparison with the control group. Number of Lymphocytes Table 6 shows that the number of lymphocytes in groups 1 and 2 decreased in the period between days 0 and 20, but this decrease was more significant in the sodium selenite group (the P value was <0.001 in each of the three groups). The statistical survey in group 1 (nano-selenium) showed that there was a significant decrease in lymphocyte counts on the 10th, 20th, and 30th day in comparison with day 0 (the P value was <0.001 in each of the three groups), on the 20th day in comparison with the 10th day (P= 0.004), and on the 30th day in comparison with the 10th day (P= 0.030). In group 2 (sodium selenite), there was significant decrease on the 10th, 20th, and 30th day in comparison with day 0 (the P value was <0.001, <0.001, and 0.002, respectively) and on the 20th day in comparison with the 10th day (P= 0.003), but unlike group 1, there was no significant difference on the 30th day in comparison to the 10th day, but in group 2, there was significant increase on the 30th day in comparison to the 20th day (P= 0.047). In the control group, there were no significant differences in the lymphocyte counts on any of the sampling days, neither with the basal level (day 0) nor with each other. By comparing the means of the three groups together, it was determined that there were no significant differences in the lymphocyte counts on day 0 (P> 0.05). The lymphocyte counts in group 1 showed a significant decrease on the 10th day in comparison with those in group 2 (P= 0.010) and the controls (P= 0.035) and in group 1 on the 20th and 30th day, in comparison with the control group, with P values of <0.001 and <0.001, respectively, and in group 2 on the 20th and 30th day, in comparison with the control group, with P values of 0.002 and 0.010, respectively.