292 Current Genomics, 2010, Vol. 11, No. 4 Analysis of individual components of other gene sets suppressed in transgenic myocytes after serum stimulation reveals a number of interesting regulatory genes. Genes in- volved in eye development (GO: 0048048) include the tran- scription factors VAX2, RARG, FOXL2 and SP1 and the metabolic enzymes ALDH1A1 and ALDH1A3. Genes in- volved in gut development (GO: 0048565) include many genes known to be important in developmental signaling and transcription such as TCF7, NOTCH1, NKX2-3, HOXD13, GLI2, FOXF2 and SHH (Fig. (5)). Genes involved in fluid transport (GO: 0042044) include the aquaporins described above for GO: 0006833 and are largely redundant. A role for any of these molecules in cardiac hypertrophy and heart fail- ure, to the best of our knowledge, has not yet been described. Yu et al. Analysis of individual components of gene sets that are activated in the CHF1/Hey2 transgenic myocytes in both the absence or presence of serum (GO: 0006470, GO: 0016311) reveals a number of phosphatases, such as PTPN4, DUSP28, MTMR6, PPP3R1, DUSP8, PPP6C, PPM1B, PPP2R4, PPP2R2A and PTPN2 (Fig. (6)). DUSP8 and PPM1B can turn off components of the cellular stress response [24, 25], while PTPN2 can attenuate EGF signaling [24, 25], both which may be relevant to hypertrophy. Fig. (5). Heat map for genes pertaining to gut development (GO: 0048565). Columns represent microarray data obtained from inde- pendent primary cultures of cells of the indicated genotype and therefore represent biological replicates. Gene name abbreviations for each component of the gene set are indicated at the end of each row. Green indicates expression is lower, while red indicates ex- pression is higher than average of WT. The genes in this set are predominantly downregulated in the transgenic cells (TgS1/2/3/4/5/ 6/7) in the presence of serum relative to the WT cells treated with serum (WTS1/2/3/4/5/6/7). Fig. (6). Heat map for genes linked to protein amino acid dephos- phorylation (GO: 0006470). Columns represent microarray data obtained from independent primary cultures of cells of the indicated genotype and therefore represent biological replicates. Gene name abbreviations for each component of the gene set are indicated at the end of each row. Green indicates expression is lower, while red indicates expression is higher than average of WT. The genes in this set are predominantly upregulated in the transgenic cells (Tg1/2/3/4/5/7) in the absence of serum relative to the correspond- ing WT cells (WT 1/2/5/6/7).