Histochem Cell Biol (2008) 129:735–749 741 Fig. 1 MDBs are readily detected by diVerent methods. In clinical routine, MDBs are usually detected as eosinophilic aggregates in stan- dard hematoxylin and eosin stained sections (a). After chromotrope aniline blue staining, MDBs appear as blue structures, often with red center (b). ImmunoXuorescence or immunohistochemical staining rep- resents a more sensitive method for MDB detection than conventional histological stainings, but is strongly dependent on the antibody used as well as the staining protocol. MDBs can be reliably detected with antibodies against K8/K18 [green and red channel in (c) and (d), respectively] or p62 [red channel in (c)], whereas only some MDBs stain with antibodies to phosphorylated keratins such as K8 pS431 antibody [green channel in (d)]. In both immunoXuorescence pictures, MDBs are seen as yellow structure due to co-localization of both visu- alized epitopes Fig. 2 MDBs are seen in vari- ous human liver diseases. Immu- nohistochemical staining with p62 antibody visualizes the pres- ence of multiple irregularly shaped aggregates in patients with alcoholic steatohepatitis (a), non-alcoholic steatohepati- tis (b), Indian childhood cirrho- sis and (c), idiopathic copper toxicosis (d) 123