Fig. 5. Section in situ hybridization of genes enriched in single M u¨ller glia. Top: Heatmap of M u¨ller glia enriched genes. A–N: ISH signal of candidate M u¨ller glial transcripts on mouse retinal sections. Gene name is as indicated. Probes used are listed in the supplemen- tary data. O: Xgal histochemistry on GPR37tm1Dgen retinal sections. P: Recombinase-dependent nuclear lacZ expression in M u¨ller glial cells of Pdgfra-Cre; RC::PFwe retinal sections. Q: Immunohistochem- istry on mouse retinal sections with anti-Pax6 (red) and anti-Glul (green) antibodies. R: Digital magniﬁcation of image shown in Q. RGCs, retinal ganglion cells; ACs, amacrine cells; PRs, photorecep- tors; MGs, Mu¨ ller glia cells; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. See Supplemental Figure S3 for magenta-green versions of Q and R. Scale bar 50 m in A (applies to A–P); 20 m in Q.