Int. J. Environ. Res. Public Health 2010, 7 3. Results 3.1. Surface Fungal Growth 3229 All 20 aged faecal pellets collected and examined for surface fungal growth supported extensive, visible, sporulating microfungal colonies (Figure 2a,b). The genera identified on the five pellets selected at random for colony characterisation included Aspergillus (three species), Penicillium (two species), Absidia, Memnoniella, Stilbella, Fusarium, Acremonium, Syncephalastrum and Geotrichum. Figure 2. (a,b) Two aged faecal pellets of Litoria caerulea covered with extensive growth of sporulating microfungi. (c) Soil particles from a faecal pellet of Litoria caerulea. (d) Microfungal spores from a needle-point fragment of the interior of a fresh faecal pellet of Litoria caerulea showing visible microfungal spores. 3.2. Microfungi Recovered from Excrement Most of the faecal pellets from which needle-point samples were taken and examined microscopically in May 2009 contained visible microfungal spores (Figure 2d). Many of the faecal pellets contained visible grass fragments and soil particles (Figure 2a,c). The estimated CFU per gram of excrement and characterisation of filamentous microfungi and yeasts, based on the colony recoveries from the decimal dilution series made from the 10 pellets collected in May 2009 and the five collected in September, 2009 are in Table 1. 3.3. Yeast Identification—Excrement The 10 yeast isolates sub-cultured from colonies recovered from the five frog pellets collected in Sept/Oct 2009 were identified as 1 Rhodotorula sp., 6 Trichosporon spp., 1 Candida sp., 1 Candida famata, and 1 Candida glabrata.