D746 Nucleic Acids Research, 2010, Vol. 38, Database issue Table 2. PSI empty vectors available at DNASU Vector name Tags Bacterial expression vectors pSpeedET modiﬁed His p11 6xHis p15Tv-LIC 6xHis p283 6xHis pDB.GST GST pDB.HBP HBP pDB.His.MBP 6xHis and MBP pDB.His.NusA 6xHis and NusA pDB.His.TRX 6xHis and thioredoxin pDB.TRX Thioredoxin pDB-HisGST 6xHis and GST pH3c-LIC 8xHis pHM3C-LIC 6xHis and MBP pHM6g 6xHis and MBP pLIC-CH 8xHis pLIC-EGFP 8xHis and EGFP pLIC.HM6g 6xHis and MBP pLICB.H 6xHis pLICB.HM 6xHis and MBP pLICB.HV 6xHis pNYCOMPS-LIC- 10xHis and Flag FH10T + (N term) pVP13a Stag, 6xHis and MBP pVP16a 8xHis and MBP pVP33Ab 8xHis and MBP pVP33Kb 6xHis and MBP pVP56Ab 8xHis and MBP pVP56Kb 8xHis and MBP pMCSG7c 6xHis Cell free expression vectors pEU-GST-FVd GST pEU-His-FVd 6xHis Coexpression vectors pMCSG11 pMCSG12 pMCSG13 pMCSG14 pMCSG17 pMCSG20 pMCSG21 pMCSG22 pMCSG23 pMCSG24 6xHis 6xHis tag and Sloop 6xHis and MBP 6xHis and GST Stag Stag and GST 6xHis 6xHis and Sloop 6xHis and MBP 6xHis and GST Vectors for increased solubility pMCSG9e 6xHis and MBP pMCSG10 6xHis and GST pMCSG19e 6xHis and MBP pMCSG19C Screening vectors pMCSG18 6xHis and MBP 6xHis and GFP Vectors to decrease protein toxicity pMCSG8 6xHis and Sloop Cloning vectors pMCentr1 attL1-TEV-LIC site-attL2 pMCentr2 pMCentr3 attL1-TEV-LIC site-attL2 attL1-TVMV-LIC site-attL2 Cloning method PIPE cloning Restriction enzyme LIC Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme LIC LIC restriction enzyme LIC LIC LIC-SmaI LIC-SmaI LIC-SmaI LIC-SmaI LIC Recombinational Recombinational Restriction enzyme Restriction enzyme Restriction enzyme Restriction enzyme LIC Restriction enzyme Restriction enzyme LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC LIC to make entry vector for recombinational cloning LIC to make entry vector for recombinational cloning Destination vector; LIC to recombinational cloning Cleavage sites TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease 3C protease 3C protease TEV protease TEV protease TEV protease TEV protease none TEV protease TEV protease TEV protease TEV protease TEV protease 3CP and TEV cleavage 3CP and TEV cleavage 3CP and TEV cleavage 3CP and TEV cleavage TEV protease 3CP cleavage None TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV protease TEV and TVMV protease TEV and TVMV protease TEV protease TEV protease TEV protease TEV protease TVMV protease Resistance Kanamycin Ampicillin Ampicillin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Kanamycin Ampicillin Ampicillin Ampicillin Ampicillin Kanamycin Ampicillin Ampicillin Ampicillin Kanamycin Ampicillin Kanamycin Ampicillin Ampicillin Ampicillin Chloroamphenicol Chloroamphenicol Chloroamphenicol Chloroamphenicol Ampicillin Ampicillin Spectinomycin Spectinomycin Spectinomycin Spectinomycin Ampicillin Ampicillin Ampicillin Ampicillin Ampicillin Ampicillin Zeocin Kanamycin Kanamycin PSI Site JCSG MCSG MCSG MCSG BSGC BSGC BSGC BSGC BSGC BSGC BSGC CSMP CSMP BSGC CSMP CSMP BSGC BSGC BSGC BSGC NYCOMPS CESG CESG CESG CESG CESG CESG MCSG CESG CESG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG MCSG His, Histidine tag; PIPE, Polymerase Incomplete Primer Extension; MBP, Maltose binding protein tag; LIC, ligation independent cloning; C3P, human rhinovirus (HRV) 3C protease cleavage site; TEV, Tobacco Etch Virus protease cleavage site; GST, Glutathione D-transferase tag; GFP, green ﬂuorescent protein tag; Sloop, Loop of GroEX that binds GroEL; TVMV, Tobacco Vein Mottling Virus Protease; attL1 or attL2, Gateway recombinational cloning sites; Stag, Small T antigen; JCSG, Joint Center for Structural Genomics; MCSG, Midwest Center for Structural Genomics; BSGC, Berkeley Structural Genomics Center; NYCOMPS, New York Consortium on Membrane Protein Structure; CESG, Center for Eukaryptoc Structural Genomics; CSMP, Center for Structures of Membrane Proteins. aRef. (20); bRef. (17); cRef. (19); dRef. (21); eRef. (18).