Zhu et al. Journal of Experimental & Clinical Cancer Research 2010, 29:124 http://www.jeccr.com/content/29/1/124 Table 1 The sequences of the primers used in the experiment Gene HIF1a Flk-1 VEGF Cyclin D1 V-src P53 b-actin Sense TGCACAGGCCACATTCACGT ACAGTGGTATGGTTCTTGCCTCA TCACCAAGGCCAGCACATAG GATGCCAACCTCCTCAACGAC CACTCGCTCAGCACAGGACAG GCTGCTCAGATAGCGATGGTC CCTGTACGCCAACACAGTGC Antisense GTTCACAAATCAGCACCAAGC GTAGCCGCTTGTCTGGTTTGA GGGAACGCTCCAGGACTTAT CTCCTCGCACTTCTGTTCCTC AGAGGCAGTAGGCACCTTTCG CTCCCAGGACAGGCACAAACA ATACTCCTGCTTGCTGATCC Page 4 of 8 Product (bps) 97 140 166 171 196 298 211 and those of apoptosis were increased after 3 d’s incuba- tion, however, there had no difference in S-phase and apoptosis after 7 d’s incubation of the two cell lines. On the other hand, the percent of S-phase of HUVEC cells was decreased and that of apoptosis was increased after both 3 and 7 d’s incubation. The numbers of cell migrated through basement membrane of the transwell chamber were shown in Fig. 3D (after 3 d’s incubation) and 3E (after 7 d’s incuba- tion). Compared to normoxia control, the numbers decreased significantly in SKOV-3 after 3 and 7 d’s incubation under hypoxia while it decreased significantly in ES-2 only after 3 d’s incubation. The numbers of HUVEC cells were decreased significantly after both 3 and 7 d’s incubation. The activities of telomerase of SKOV-3, ES-2 and HUVEC cells are changed by hypoxia In order to study the malignant of the ovarian cancer cells, the activities of telomerase of SKOV-3, ES-2 and Figure 1 The morphology of the ELs from ovarian cancer induced by hypoxia and microdissected by LCM. The ovarian cancer cells were cultured in 3-dimisonal Matrigel system on EVA membrane under hypoxia for 7 d before harvest. The pictures were taken under the light microscope. A and B. The original and after microdissected by LCM of the single cell. C and D. The original and after microdissected by LCM of the grouped cells. Magnification X200. Arrow: The morphology of the cells after microdissection.