Yu et al. Journal of Experimental & Clinical Cancer Research 2010, 29:107 http://www.jeccr.com/content/29/1/107 1.3 Statistical Analysis The experimental result indicated by the mean ± stan- dard deviation(x¯ ± S), used the SPSS11.5 statistics soft- ware analysis system to carry on the c2-test, and the T- test used for categorized variables and the Spearman rank used for continuous variables correlation analysis. It is considered to be statistically significant difference when P < 0.05. Results 2.1.1 The expression of BCL-2, BAD in breast carcinoma, breast fibroadenoma and normal breast tissues The expression of BCL-2 and BAD gene in breast carci- noma tissues were indicated by brown granules, mainly distributes in the cytoplasma, and non-uniform; The positive expression of BCL-2 and BAD in breast carci- noma(Fig 1, 2). The expression rates of BCL-2 were nor- mal breast tissue(90%), breast fibroadenoma(80%) and breast carcinoma(61.25%). Compare with the other 2 groups, the expression of BCL-2 was higher in breast carcinoma group, the differences were statistically signif- icant(P < 0.05) (Table 1). Figure 1 The positive immunohistochemical expression of BCL- 2 in breast carcinoma tissue. Page 3 of 7 Figure 2 The positive immunohistochemical expression of BAD in breast carcinoma tissue. 2.1.2 The expression of BCL-2, BAD in youth and menopause human breast carcinoma The expression rates of BCL-2 in youth and menopause human breast carcinoma were 47.5% and 75%(P < 0.05), The expression rates of BAD in youth and menopause human breast carcinoma were 30% and 65%, the differ- ences were statistically significant(P < 0.05) (P < 0.05). (Table 2) Table 1 The expression of BCL-2, BAD in breast carcinoma, breast fibroadenoma and normal breast tissue Normal breast tissue Breast fibroadenoma Breast carcinoma Total 10 10 80 + BCL-2 - +(%) BAD + - +(%) 9 1 90.00% 8 2 80.00% 8 2 80.00% 7 3 70.00% 49 31 61.25%a 38 42 47.50%b Compare with the other two groups a P < 0.05; b P < 0.05