Histochem Cell Biol (2012) 138:861–879 867 was barely detected in 4VCP from E19 rats, and the band increased in intensity in older animals (Fig. 4a). Cld-2 immunoreactivity was associated with all intercellular epithelial junctions in LVCP of E19 rats, but displayed a patchy, discontinuous pattern of lower intensity in 4VCP of the same animals (Fig. 4b). The difference between LVCP 123