Sule Menziletoglu Yildiz, Sedefgul Yuzbasioglu Ariyurek, Murat Tahiroglu, Kiymet Aksoy 0.75 units of Taq polymerase and 100 ng of genomic DNA in a total volume of 25 μl. Thermocycling was performed on a Perkin Elmer 9600 system (Norwalk, Connecticut, USA) using the following conditions: Subsequent to denaturation at 95°C for 5 min, 35 cycles consisted of 1 min at 95°C, 1 min at 60°C, 1 min at 72°C, and a final extension at 72°C for 10 min were performed. After purification from excess unincorporated primers and desalting, the PCR products were mixed with histidine, and then electronically addressed. Afterwards, the amplified products were denatured using 0.1 M sodium hydroxide for 3 min and then the cartridge was incubated with stabilizers in a high salt buffer for another 3 min. Following hybridization of probes, a thermal stringency step specific for 1311 polymorphism was carried out in the Reader and hybridization was detected through fluorescence using automated scanning and dedicated software. Restriction fragment length polymorphism (RFLP) was used to confirm the C1311T mutation. Table I. The fluorescence signal rates from microarray analysis and the genotype of 1311 polymorphism in the G6PD gene Case no. and sex 1. Gd 1311 Control 2. M 3. F 4. M 5. F 6. F 7. M 8. M 9. M 10. M 11. F 12. F 13. F 14. F 15. F 16. M 17. M 18. M 19. F 20. M 21. F 22. M 23. M 24. M 25. M 26. M 27. F 28. M 29. M 30. M 31. M 32. F 33. M 588 Red 237 39 42 99 2 0 272 14 0 0 0 51 259 418 15 436 311 261 317 282 207 174 197 7 296 235 304 162 188 232 246 155 15 Green 3.33 4.43 280.5 17.74 1331.54 1579.89 48.31 900.26 2197.43 282.72 1637.54 51 11.09 14.41 2734.04 85.37 16.63 9.98 14.41 9.98 14.41 9.98 6.65 1544.41 35.48 9.98 6.65 6.65 6.65 7.76 6.65 838.55 88.7 Rate (R/G) 21.72 8.79 : 1 1 : 6.68 5.58 : 1 1 : 665.77 Endless 5.63 : 1 1 : 64.3 Endless Endless Endless 1 : 1 23.36 : 1 29 : 1 1 : 182.27 5.11 : 1 18.7 : 1 26.16 : 1 23.83 : 1 28.26 : 1 14.36 : 1 17.44 : 1 29.61 : 1 1 : 220.63 8.34 : 1 23.55 : 1 45.7 : 1 24.35 : 1 28.26 : 1 29.89 : 1 3698 : 1 1 : 5.41 1 : 5.91 Prob Red/red Red/red Green/green Red/red Green/green Green/green Red/red Green/green Green/green Green/green Green/green Red/green Red/red Red/red Green/green Red/red Red/red Red/red Red/red Red/red Red/red Red/red Red/red Green/green Red/red Red/red Red/red Red/red Red/red Red/red Red/red Green/green Green/green Genotype Control Hemizygous Normal Hemizygous Normal Normal Hemizygous Normal Normal Normal Normal Heterozygous Homozygous Homozygous Normal Hemizygous Hemizygous Hemizygous Homozygous Hemizygous Homozygous Hemizygous Hemizygous Normal Hemizygous Hemizygous Homozygous Hemizygous Hemizygous Hemizygous Hemizygous Normal Normal Arch Med Sci 4, August / 2011