Journal of Experimental & Clinical Cancer Research 2008, 27:87 http://www.jeccr.com/content/27/1/87 TFhigeuerxep2ression levels of BCR/ABL, mTOR and PTEN mRNA in K562 cells transfected with PTEN gene (MOI = 200) The expression levels of BCR/ABL, mTOR and PTEN mRNA in K562 cells transfected with PTEN gene (MOI = 200). *mTOR to right ordinate When the expression of PTEN mRNA increased, the expression of mTOR mRNA decreased in K562 cells after the cells transfected wild-type PTEN, but BCR/ABL fusion gene did not change significantly. 60kD 60kD 54kD p-Akt Total Akt PTEN 0d 1d 3d 5d 7d FAikgtu,pre-A3kt and PTEN protein expression levels in K562 cells transfected with PTEN for different time point Akt, p-Akt and PTEN protein expression levels in K562 cells transfected with PTEN for different time point. The expression of p-Akt- protein in K562 cells decreased gradually following Ad-GFP-PTEN transfection, and the lowest level appeared on the third day of the transfection. PTEN protein increased gradually after Ad-GFP-PTEN transfection, and the top level was detected on the third day of the transfection. However, there was no obvious change in total Akt expression. Page 5 of 9 (page number not for citation purposes)