1700 A. S. St u¨rzebecher and others J Physiol 588.10 Figure 2. Cell-autonomous inhibition of sodium currents in nociceptors of Tg-t-MrVIa mice A, co-expression of Scn10a and t-MrVIa transcripts in DRGs of Tg-t-MrVIa mice detected by RT-PCR analysis (Sk, skin; Bs, brain stem; Co, cortex. B, in situ hybridisation on spinal cord sections of mouse embryos (E15) show strong t-MrVIa signal in DRGs but not in spinal cord (Sc) of Tg-t-MrVIa mice. Vc, vertebral column. Scale bar: 100 μm. C, t-MrVIa transcripts were detected by in situ hybridisation in nociceptors (arrows) but not mechano- receptors (arrowheads) of Tg-t-MrVIa mice (2–4 weeks old). Scale bar: 50 μm. D–H, voltage-gated currents were evoked by step depolarisations from −50 mV to +40 mV preceded by a hyperpolarizing prepulse from −60 to −120 mV to prevent inactivation. D and E, representative traces and current–voltage relationships of inward currents indicate a signiﬁcant reduction of sodium currents in nociceptors of Tg-t-MrVIa mice in comparison to wild-type littermates. F, current–voltage relations of outward currents are not affected in nociceptors of Tg-t-MrVIa mice. G and H, representative traces and current–voltage relations of inward currents, evoked by the same protocol shown in D, indicate no differences in mechanoreceptors of Tg-t-MrVIa mice and wild-type littermates. I, peak C 2010 The Authors. Journal compilation C 2010 The Physiological Society