12. Ballestero S, Virseda I, Escobar H, Suarez L, Baquero F. Stenotrophomo- nas maltophilia in cystic fibrosis patients. Eur J Clin Microbiol Infect Dis 1995;14:728–9. 13. Denton M. Stenotrophomonas maltophilia: an emerging problem in cystic fibrosis patients. Rev Med Microbiol 1997;8:15–9. 14. Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. Microbial biofilms. Annu Rev Microbiol 1995;49:711–45. 15. Mills AL, Powelson DK. Bacterial interactions with surfaces in soil. In: Fletcher M, editor. Bacterial adhesion: molecular and ecological diversity. New York: John Wiley & Sons; 1996. p. 25–7. 16. Leriche V, Sibille P, Carpentier B. Use of an enzyme-linked lectinsorbent assay to monitor the shift in polysaccharide composition in bacterial bio- films. Appl Environ Microbiol 2000;66:1851–6. 17. O’Brien M, Davis GHG. Enzymatic profile of Pseudomonas maltophilia. J Clin Microbiol 1982;16:417–21. 18. O’Toole GA, Kolter R. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol 1998;30:295–304. 19. La Ragione RM, Sayers AR, Woodward MJ. The role of fimbriae and fla- gella in the colonization, invasion and persistence of Escherichia coli O78:K80 in the day-old chick model. Epidemiol Infect 2000;124:351–63. 20. Pratt LA, Kolter R. Genetic analysis of Escherichia coli biofilm forma- tion: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol 1998;30:285–93. 21. Sheikh JM, Hicks S, Dall’Agnol M, Phillips A, Nataro JP. Role for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli. Mol Microbiol 2001;41:983–98. 22. Watnick PI, Kolter R. Steps in the development of a Vibrio cholerae El Tor biofilm. Mol Microbiol 1999;34:586–95. 23. Jucker BA, Harms H, Zehnder AJB. Adhesion to positively charged bac- terium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and Teflon. J Bacteriol 1996;178:5472–9. RESEARCH 24. Young GM, Badger JL, Miller VL. Motility is required to initiate host cell invasion by Yersinia enterocolitica. Infect Immun 2000;68:4323–6. 25. Garcia DO, Timenetsky J, Martinez MB, Francisco W, Sinto SI, Yanagu- ita RM. Proteases (caseinase and elastase), hemolysins, adhesion and sus- ceptibility to antimicrobials of Stenotrophomonas maltophilia isolates obtained from clinical specimens. Braz J Microbiol 2002;33:1643–54. 26. Girón JA. Expression of flagella and motility by Shigella. Mol Microbiol 1995;18:63–75. 27. Girón JA, Ho SY, Schoolnik GK. An inducible bundle-forming pilus pro- duced by enteropathogenic Escherichia coli. Science 1991;254:710–3. 28. Laemmli UK. Cleavage of strutural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–5. 29. Mobley HT, Belas B, Lockatell V, Chippendale G, Trifillis AL, Johnson DE, et al. Construction of a flagellum-negative mutant of Proteus mirabi- lis: effect on internalization by human renal epithelial cells and virulence in a mouse model of ascending urinary tract infections. Infect Immun 1996;64:5332–40. 30. Chilcott GS, Hughes K. Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli. Microbiol Mol Biol Rev 2000;64:694–08. 31. MacNab RM. Flagella and motility. In: Neidhardt FC, editor. Escherichia coli and Salmonella cellular and molecular biology. Vol. 1. Washington: ASM Press; 1996. p 123–45. 32. Montie TC, Stover GB. Isolation and characterization of flagellar prepa- rations from Pseudomonas species. J Clin Microbiol 1983;18:452–6. Address for correspondence: Jorge A. Giron, Centro de Investigaciones en Ciencias Microbiologicas, Benemerita Universidad Autonoma de Puebla, Edi- ficio 76, Ciudad Universitaria, CP 72000, Puebla, Puebla, Mexico; fax: 52 22 244 45 18; e-mail: jagiron@yahoo.com Search past issues of EID at www.cdc.gov/eid Emerging Infectious Diseases • Vol. 8, No. 9, September 2002 923