20 Zeichner-David M. Regeneration of periodontal tissues: cementogenesis revisited. Periodontol 2000 2006; 41: 196–217. 21 Fong H, Chu EY, Tompkins KA et al. Aberrant cementum phenotype associated with the hypophosphatemic hyp mouse. J Periodontol 2009; 80(8): 1348–1354. 22 Foster BL, Nagatomo KJ, Bamashmous SO et al. The progressive ankylosis protein regulates cementum apposition and extracellular matrix composition. Cells Tissues Organs 2011; 194(5): 382–405. 23 Jacquet R, Hillyer J, Landis WJ. Analysis of connective tissues by laser capture microdissection and reverse transcriptase–polymerase chain reaction. Anal Biochem 2005; 337(1): 22–34. 24 Kai N, Iwase K, Imai K et al. Altered gene expression in the subdivisions of the amygdala of Fyn-deficient mice as revealed by laser capture microdissection and mKIAA cDNA array analysis. Brain Res 2006; 1073–1074: 60–70. 25 Liu D, Yao S, Wise GE. Effect of interleukin-10 on gene expression of osteoclastogenic regulatory molecules in the rat dental follicle. Eur J Oral Sci 2006; 114(1): 42–49. 26 Nakamura Y, Nomura Y, Arai C et al. Laser capture microdissection of rat periodontal ligament for gene analysis. Biotech Histochem 2007; 82(6): 295–300. 27 Wise GE, Ding D, Yao S. Regulation of secretion of osteoprotegerin in rat dental follicle cells. Eur J Oral Sci 2004; 112(5): 439–444. 28 Yao S, Ring S, Henk WG et al. In vivo expression of RANKL in the rat dental follicle as determined by laser capture microdissection. Arch Oral Biol 2011; 49(6): 451– 456. 29 Yao S, Pan F, Wise GE. Chronological gene expression of parathyroid hormone-related protein (PTHrP) in the stellate reticulum of the rat: implications for tooth eruption. Arch Oral Biol 2007; 52(3): 228–232. 30 Fong HK, Foster BL, Popowics TE et al. The crowning achievement: getting to the root of the problem. J Dent Educ 2005; 69(5): 555–570. 31 Foster BL, Somerman MJ. Regenerating the periodontium: is there a magic formula? Orthod Craniofac Res 2005; 8(4): 285–291. 32 Foster BL, Popowics TE, Fong HK et al. Advances in defining regulators of cementum development and periodontal regeneration. Curr Top Dev Biol 2007; 78: 47–126. 33 D’Errico JA, MacNeil RL, Takata T et al. Expression of bone associated markers by tooth root lining cells, in situ and in vitro. Bone 1997; 20(2): 117–126. 34 Kawano S, Saito M, Handa K et al. Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor. J Dent Res 2004; 83(2): 129–133. 35 Klein OD, Lyons DB, Balooch G et al. An FGF signaling loop sustains the generation of differentiated progeny from stem cells in mouse incisors. Development 2008; 135(2): 377–385. 36 Yamashiro T, Tummers M, Thesleff I. Expression of bone morphogenetic proteins and Msx genes during root formation. J Dent Res 2003; 82(3): 172–176. 37 Macneil RL, Sheng N, Strayhorn C et al. Bone sialoprotein is localized to the root surface during cementogenesis. J Bone Miner Res 1994; 9(10): 1597–1606. 38 Lee NK, Sowa H, Hinoi E et al. Endocrine regulation of energy metabolism by the skeleton. Cell 2007; 130(3): 456–469. 39 Kawamoto T, Shimizu M. A method for preparing 2- to 50-micron-thick fresh-frozen sections of large samples and undecalcified hard tissues. Histochem Cell Biol 2000; 113(5): 331–339. 40 Meacock SC, Brandon DR, Brown CP et al. A novel technique for immuno- histoperoxidase staining of unfixed whole joints of small animals. Histochem J 1992; 24(2): 115–119. 41 Clement-Ziza M, Munnich A, Lyonnet S et al. Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions. Rna 2008; 14(12): 2698–2704. Laser capture microdissection for root study JX Sun et al 42 Guo D, Catchpoole DR. Isolation of intact RNA following cryosection of archived frozen tissue. Biotechniques 2003; 34(1): 48–50. 43 Roos-van Groningen MC, Eikmans M, Baelde HJ et al. Improvement of extraction and processing of RNA from renal biopsies. Kidney Int 2004; 65(1): 97–105. 44 Cummings M, McGinley CV, Wilkinson N et al. A robust RNA integrity-preserving staining protocol for laser capture microdissection of endometrial cancer tissue. Anal Biochem 2011; 416(1): 123–125. 45 Wang WZ, Oeschger FM, Lee S et al. High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection. BMC Mol Biol 2009; 10: 69. 46 Casagrande L, Demarco FF, Zhang Z et al. Dentin-derived BMP-2 and odontoblast differentiation. J Dent Res 2010; 89(6): 603–608. 47 Chen S, Gluhak-Heinrich J, Martinez M et al. Bone morphogenetic protein 2 mediates dentin sialophosphoprotein expression and odontoblast differentiation via NF-Y signaling. J Biol Chem 2008; 283(28): 19359–19370. 48 Nakashima M. Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy. Cytokine Growth Factor Rev 2005; 16(3): 369–376. 49 Yang X, van der Kraan PM, Bian Z et al. Mineralized tissue formation by BMP2- transfected pulp stem cells. J Dent Res 2009; 88(11): 1020–1025. 50 de Vega S, Iwamoto T, Yamada Y. Fibulins: multiple roles in matrix structures and tissue functions. Cell Mol Life Sci 2009; 66(11/12): 1890–1902. 51 Zhang HY, Timpl R, Sasaki T et al. Fibulin-1 and fibulin-2 expression during organogenesis in the developing mouse embryo. Dev Dyn 1996; 205(3): 348–364. 52 Tsuda T, Wang H, Timpl R et al. Fibulin-2 expression marks transformed mesenchymal cells in developing cardiac valves, aortic arch vessels, and coronary vessels. Dev Dyn 2001; 222(1): 89–100. 53 Ehlermann J, Weber S, Pfisterer P et al. Cloning, expression and characterization of the murine Efemp1, a gene mutated in Doyne–Honeycomb retinal dystrophy. Gene Expr Patterns 2003; 3(4): 441–447. 54 Lotery AJ, Baas D, Ridley C et al. Reduced secretion of fibulin 5 in age-related macular degeneration and cutis laxa. Hum Mutat 2006; 27(6): 568–574. 55 Schultz DW, Klein ML, Humpert AJ et al. Analysis of the ARMD1 locus: evidence that a mutation in Hemicentin-1 is associated with age-related macular degeneration in a large family. Hum Mol Genet 2003; 12(24): 3315–3323. 56 Bri o´n M, Sanchez-Salorio M, Cort o´n M et al. Genetic association study of age-related macular degeneration in the Spanish population. Acta Ophthalmol 2011; 89(1): e12– e22. 57 Williamson MR, Shuttleworth A, Canfield AE et al. The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment. Biomaterials 2007; 28(35): 5307–5318. 58 Vogel BE, Hedgecock EM. Hemicentin, a conserved extracellular member of the immunoglobulin superfamily, organizes epithelial and other cell attachments into oriented line-shaped junctions. Development 2001; 128(6): 883–894. 59 de Vega S, Iwamoto T, Nakamura T et al. TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding. J Biol Chem 2007; 282(42): 30878–30888. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 Unported License. To view a copy of this license, visit http:// creativecommons.org/licenses/by-nc-nd/3.0 13 International Journal of Oral Science