Ba¨r H, Mu¨cke N, Kostareva A, Sjoberg G, Aebi U, Herrmann H. 2005. Severe muscle disease-causing desmin mutations interfere with in vitro ﬁlament assembly at distinct stages. Proc Natl Acad Sci USA 102 (42): 15099–15104. Ba¨r H, Mu¨cke N, Ringler P, Mu¨ller SA, Kreplak L, Katus HA, Aebi U, Herrmann H. 2006. Impact of disease mutations on the desmin ﬁlament assembly process. J Mol Biol 360 (5): 1031–1042. Ba¨r H, Schopferer M, Sharma S, Hochstein B, Mu¨cke N, Herr- mann H, Willenbacher N. 2010. Mutations in desmin’s carboxy-ter- minal ‘‘tail" domain severely modify ﬁlament and network mechanics. J Mol Biol 397 (5): 1188–1198. Becker E, Herrera NC, Gunderson FQ, Derman AI, Dance AL, Sims J, Larsen RA, Pogliano J. 2006. DNA segregation by the bac- terial actin AlfA during Bacillus subtilis growth and development. EMBO J 25 (24): 5919–5931. Beuttenmu¨ller M, Chen M, Janetzko A, Ku¨hn S, Traub P. 1994. Structural elements of the amino-terminal head domain of vimentin essential for intermediate ﬁlament formation in vivo and in vitro. Exp Cell Res 213 (1): 128–142. Bork P, Sander C, Valencia A. 1992. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc Natl Acad Sci USA 89 (16): 7290–7294. Cabeen MT, Charbon G, Vollmer W, Born P, Ausmees N, Weibel DB, Jacobs-Wagner C. 2009. Bacterial cell curvature through me- chanical control of cell growth. EMBO J 28 (9): 1208–1219. Cabeen MT, Murolo MA, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C. 2010. Mutations in the lipopoly- saccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus. J Bacteriol 192 (13): 3368–3378. Cabeen MT, Jacobs-Wagner C. 2010. The bacterial cytoskeleton. Annu Rev Genet 44:365–392. Charbon G, Cabeen MT, Jacobs-Wagner C. 2009. Bacterial inter- mediate ﬁlaments: in vivo assembly, organization, and dynamics of crescentin. Genes Dev 23 (9): 1131–1144. de Boer P, Crossley R, Rothﬁeld L. 1992. The essential bacterial cell-division protein FtsZ is a GTPase. Nature 359 (6392): 254–256. Delorenzi M, Speed T. 2002. An HMM model for coiled-coil domains and a comparison with PSSM-based predictions. Bioinfor- matics 18 (4): 617–625. Derman AI, Becker EC, Truong BD, Fujioka A, Tucey TM, Erb ML, Patterson PC, Pogliano J. 2009. Phylogenetic analysis identiﬁes many uncharacterized actin-like proteins (Alps) in bacteria: regu- lated polymerization, dynamic instability and treadmilling in Alp7A. Mol Microbiol 73 (4): 534–552. Eckelt A, Herrmann H, Franke WW. 1992. Assembly of a tail-less mutant of the intermediate ﬁlament protein, vimentin, in vitro and in vivo. Eur J Cell Biol 58 (2): 319–330. Ely B. 1991. Genetics of Caulobacter crescentus. Methods Enzymol 204:372–384. Erickson HP. 2007. Evolution of the cytoskeleton. Bioessays 29 (7): 668–677. Esue O, Rupprecht L, Sun SX, Wirtz D. 2010. Dynamics of the bacterial intermediate ﬁlament crescentin in vitro and in vivo. PLoS One 5 (1): e8855. Evinger M, Agabian N. 1977. Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells. J Bacteriol 132 (1): 294–301. Geisler N, Weber K. 1988. Phosphorylation of desmin in vitro inhibits formation of intermediate ﬁlaments; identiﬁcation of three n 218 Cabeen et al. kinase A sites in the aminoterminal head domain. EMBO J 7 (1): 15–20. Gitai Z, Dye N, Shapiro L. 2004. An actin-like gene can determine cell polarity in bacteria. Proc Natl Acad Sci USA 101 (23): 8643–8648. Graumann PL. 2009. Dynamics of bacterial cytoskeletal elements. Cell Motil Cytoskeleton 66 (11): 909–914. Grifﬁn BA, Adams SR, Tsien RY. 1998. Speciﬁc covalent labeling of recombinant protein molecules inside live cells. Science 281 (5374): 269–272. Harborth J, Elbashir SM, Bechert K, Tuschl T, Weber K. 2001. Identiﬁcation of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Sci 114 (Part 24): 4557–4565. Hatzfeld M, Burba M. 1994. Function of type I and type II keratin head domains: their role in dimer, tetramer and ﬁlament formation. J Cell Sci 107 (Part 7): 1959–1972. Hedberg KK, Chen LB. 1986. Absence of intermediate ﬁlaments in a human adrenal cortex carcinoma-derived cell line. Exp Cell Res 163 (2): 509–517. Heitlinger E, Peter M, Lustig A, Villiger W, Nigg EA, Aebi U. 1992. The role of the head and tail domain in lamin structure and assembly: analysis of bacterially expressed chicken lamin A and truncated B2 lamins. J Struct Biol 108 (1): 74–89. Herrmann H, Aebi U. 2004. Intermediate ﬁlaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular scaffolds. Annu Rev Biochem 73:749–789. Herrmann H, Haner M, Brettel M, Muller SA, Goldie KN, Fedtke B, Lustig A, Franke WW, Aebi U. 1996. Structure and assembly properties of the intermediate ﬁlament protein vimentin: the role of its head, rod and tail domains. J Mol Biol 264 (5): 933–953. Herrmann H, Haner M, Brettel M, Ku NO, Aebi U. 1999. Char- acterization of distinct early assembly units of different intermediate ﬁlament proteins. J Mol Biol 286 (5): 1403–1420. Herrmann H, Ba¨r H, Kreplak L, Strelkov SV, Aebi U. 2007. Inter- mediate ﬁlaments: from cell architecture to nanomechanics. Nat Rev Mol Cell Biol 8 (7): 562–573. Herrmann H, Strelkov SV, Burkhard P, Aebi U. 2009. Intermediate ﬁlaments: primary determinants of cell architecture and plasticity. J Clin Invest 119 (7): 1772–1783. Herrmann H, Strelkov SV, Feja B, Rogers KR, Brettel M, Lustig A, Haner M, Parry DA, Steinert PM, Burkhard P, et al. 2000. The in- termediate ﬁlament protein consensus motif of helix 2B: its atomic structure and contribution to assembly. J Mol Biol 298 (5): 817–832. Izard J. 2006. Cytoskeletal cytoplasmic ﬁlament ribbon of Trepo- nema: a member of an intermediate-like ﬁlament protein family. J Mol Microbiol Biotechnol 11 (3-5): 159–166. Jiang H, Sun SX. 2010. Morphology, growth, and size limit of bac- terial cells. Phys Rev Lett 105 (2): 028101. Lam H, Schoﬁeld WB, Jacobs-Wagner C. 2006. A landmark pro- tein essential for establishing and perpetuating the polarity of a bac- terial cell. Cell 124 (5): 1011–1023. Larsen RA, Cusumano C, Fujioka A, Lim-Fong G, Patterson P, Pogliano J. 2007. Treadmilling of a prokaryotic tubulin-like protein, TubZ, required for plasmid stability in Bacillus thuringiensis. Genes Dev 21 (11): 1340–1352. Lo¨we J, Amos LA. 1998. Crystal structure of the bacterial cell-divi- sion protein FtsZ. Nature 391 (6663): 203–206. Mukherjee A, Dai K, Lutkenhaus J. 1993. Escherichia coli cell divi- sion protein FtsZ is a guanine nucleotide binding protein. Proc Natl Acad Sci USA 90 (3): 1053–1057. CYTOSKELETON