Table 2. Summary of What’s New since our last NAR update MPD categories with new data Appearance Coat color Behavior Anxiety Exploratory Learning and memory Locomotor activity Motor coordination and activity Social Stress reactivity Wildness Blood Clinical chemistry Hematology Lipids Body composition BMI Fat pads Body metrics Size, weight, growth curve Bone Mineral density and content Metrics Brain Drug concentration Gene expression Cardiovascular Echocardiography Blood pressure, heart rate Cell and tissue damage Apoptosis Chromosome instability Ear Balance Hearing Endocrine Adrenal morphology Hormones Immune system Cell counts Immunoglobulins Response to infection Kidney Urinalysis Vesico-ureteral reﬂux Liver Pathology Homeostasis Gene expression Longevity Lifespan Metabolism Body temperature Energy Food and water intake Nervous system Autonomic function Neuron projection morphology Neurogenesis Neuromuscular function Sensorimotor function Neurosensory Nociception Sensory gating Organ metrics Eye Kidney Liver Spleen Reproduction Fecundity Female Male Sex distribution Respiratory Airway resistance Breathing pattern Lung compliance Lung volume Treatment and intervention studies [S8] (with new data) Dietary interventions Capsaicin Diet restriction High-fat diet High-fat diet+ethanol Sucrose Drugs and alcohol Acetaminophen Ethanol Fluoxetine Haloperidol Isoniazid Lithium b-adrenergic drugs Environmental insult Trichloroethylene Infectious diseases Candida Inﬂuenza A (H1N1) Malaria Lifestyle and healthspan Aging Exercise Nucleic Acids Research, 2012, Vol. 40, Database issue D889 (22%), e.g. administering drugs or high-fat diet (Interventions: S8). MPD has acquired and formatted hundreds of experimental procedures, housing a library of protocols where detailed procedures and assays are maintained so that the research community may beneﬁt from their use (Protocols: S9, S10). Genotype data The current MPD SNP database contains 350 million SNP calls (count excludes imputed SNPs) obtained from approximately 30 publicly available or contributed SNP sources, including Broad, Celera, Center for Genome Dynamics, Merck, Perlegen (NIEHS), Wellcome Trust, Novartis (GNF) and The Jackson Laboratory (MPD SNP Database: S11). MPD makes SNPs available in sets for RI panels (8 total), C57BL/6 substrains (13 variants) and 129 substrains (7 variants). In total, MPD has SNP data for >450 veriﬁed strains (Find Strains with SNPs: S2, S3). MPD combines SNP data with gene annotations from external resources such as Mouse Genome Informatics (MGI) (12), NCBI dbSNP (13) and Ensembl (14). Gene expression data In addition to new phenotypic and genotypic data, MPD has recently begun accessioning gene expression strain survey data. Currently, there are eight gene expression projects, representing 280K probesets. Four of these projects are part of a treatment/control paradigm, and phenotype data are available in parallel. These interven- tion studies are from two laboratories studying the effects of a high-fat diet [Shockley1 (15,16)] and an environmen- tal insult (trichloroethylene exposure) [Rusyn2 (17,18)]. Thus far, data are from either whole brain or liver; efforts are underway to obtain data from other tissues and cell types. Most data sets are inbred strain surveys (10–30 strains), and another is for the ILSXISS recombinant inbred strain panel (61 strains) [GX-Tabakoff2 (19)]. Functionality of the new gene expression interface is showcased below in detail (see also Gene Expression: S12). Since our last reporting, we have accessioned over 70 new phenotype or gene expression projects from over 100 investigators representing >40 institutions. See Table 2 for ‘What’s New’ in MPD categories and intervention groups. Several of these new projects are important drug studies, including acetaminophen toxicity [Threadgill1, Threadgill2 (20–23)], haloperidol effects on behavior [Crowley1 (24,25)] and effects of b-adrenergic drugs on heart function [Maurer1 (26,27)] (Projects: S6; Interventions: S8). Most have associated publications, providing assurance that the study has been peer-reviewed and the data validated by experts. FUNCTIONALITY MPD provides many options to view, analyze and download data (MPD Toolbox Demo: S13). Since our last NAR update, we have made signiﬁcant improvements