2719656 3577940 3160175 2991233 2878074 2370433 CACNA1E RUNX CD38 CLMN VLDLR AHR NRG2 Real-time PCR aFrom GSE7851. 3284596 PARD3 3848243 INSR 3721010 IGFBP4 3848243 3577940 INSR CLMN Runt-related transcription factor 1 CD38 molecule Calmin Very low density lipoprotein receptor Calcium channel, voltage-dependent, R type, alpha 1E subunit Aryl hydrocarbon receptor Neuregulin 2 Par-3 partitioning defective 3 homolog Insulin receptor Insulin-like growth factor binding protein 4 Insulin receptor Calmin Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse 50-CCTCTGCTGCCAGTAAAGAAG-30 50-Fam-CCGGCCACTTTATCACAGC-30 50-CCTGTCCAGATACTTGATACTTTC-30 50-Fam-AGTCCAGAGTGCTGCCAGT-30 50-Fam-TGCCTCGAAGGTACTTAGGAAGT-30 50-AGAAGTTTGTACGTGGGCTCTTG-30 50-AGCACACTGTACTCCTAATGCCTG-30 50-Fam-AGGACAGGCATCAGCATTTTC-30 50-Fam-AATGTTGTGTGCTGTCCCAA-30 50-TTGCATACAGTGGGCACACA-30 50-Fam-AGTTGTACAGACAACCTGGAGA-30 50-CAGTGATGTATGTGGAGAAGTAAT-30 50-TTCCGGCCTAAAGAACTAGCTC-30 50-Fam-AGACTGGCCTGGGATGTGT-30 50-GCTTTGGACACACTAGTGTCTGC-30 50-Fam-ATGAGGATCCAGAGCAGGAAA-30 50-GAAGTCTTTAGATGGCTTCTCTCTG-30 50-Fam-GCCTTATATTCTAGGTGCTGAGG-30 50-Fam-GGTGGTAGAAAAGTCTTGGCTC-30 50-GTGTAAGAATGAGGTTAGGGCAA-30 50-AAGACCATCGACTCGGTGAC-30 50-GGATCGGCGGATTTTTAGAT-30 50-GGTTCTTGGGCTGATATGGA-30 50-GGCCTTGATAGCCTTCCTCT-30 Experiment Genotyping Affymetrix transcript cluster IDa 3930360 Exploring the relationship between polymorphic (TG/CA)n repeats RESEARCH Table 1. Primers used in genotyping and real-time PCR Gene Description Direction Primer The PCR reaction mix was prepared with 1 unit of HotStart Taq Polymerase (Qiagen, Valencia, CA, USA), 1.5 mM MgCl2 (Qiagen, Valencia, CA, USA), 250 nM of each primer (IDT, San Jose, CA, USA) and 20 ng of genomic DNA. PCR ampliﬁca- tion was performed in a thermal cycler (Bio-Rad, Hercules, CA, USA) with an initial step of 12 minutes at 958C, followed by 35 cycles of 10 seconds # HENRY STEWART PUBLICATIONS 1479–7364. HUMAN GENOMICS. VOL 3. NO 3. 236–245 APRIL 2009 239