The therapeutic effect of PEMF on reproduction Kumar S et al. CLINICS 2011;66(7):1237-1245 Figure 2 - Schematic layout of the 2.45-GHz exposure device. were 0.32 mm in diameter, had a thin layer of enamel insulation (Belden, St. Louis, MO), and were framed on rectangular plastic formers (30 cm617.8 cm) with 150 turns. The four coils were spaced 9.7 cm apart from one another. The coils were connected in parallel pairs. Each pair was connected to one channel of a two-channel amplifier, and the connection leads were connected to a distribution unit that permitted individual coil-phase selection. The coils were electrically shielded with conductive silver paint and copper foil to minimize the introduction of an electric field. An in-house-built arbitrary function signal generator was configured to produce a 100 Hz (64-step) step. Prato et al36 also used the same setup. The wave shape of the PEMF in the time domain is shown in Figure 4. Statistical analysis All of the experimental results were compared with those of the sham exposure group and are expressed as means¡SEMs. The analyses were performed using GraphPad Prism software and a one-way analysis of variance. p,0.05 was considered to be significant. To test the effectiveness of the exposure, a multiple-range test was performed by using Fisher’s LSD test. The mean differences between the various experimental groups were found to be greater than the LSD values at a Figure 3 - Diagram of the mu-metal box with a pulsed electromagnetic field generator at 100 Hz. Four vertical light pipes were mounted within the enclosure (one in each corner port). These acrylic rods are removable. Light was supplied by four LED sources that fit under the lower ports. The four rectangular coils were mounted inside the mu-metal box in a Merritt-like configuration. 1240