294 M H ABEL and others . Effects of FSH on testicular transcript levels Table 1 Effects of FSH treatment on testicular transcript levels – highest-regulated transcripts from microarray studiesa Gene symbol Gene title Fold Gene change symbol Gene title Fold change Transcripts up-regulated 12 h after start of treatment 20.7 Ren1 Renin 1 structural 18.2 Dmkn Dermokine 13.3 Aqp5 Aquaporin 5 12.1 Wif1 Wnt inhibitory factor 1 11.0 Fabp5 Fatty acid binding protein 5, epidermal 6.6 Tubb3 Tubulin, b 3 6.5 Cyp17a1 Cytochrome P450, family 17a 1 6.3 Akr1b7 Aldo-keto reductase family 1 B7 6.0 Lgals1 Lectin, galactose binding, soluble 1 5.9 Col4a1 Procollagen, type IV, a1 5.7 Pappa Pregnancy-associated plasma protein A 5.2 Star Steroidogenic acute regulatory protein 4.6 Ldlr Low density lipoprotein receptor 4.6 Tesc Tescalcin 4.6 Rps6ka2 Ribosomal protein S6 kinase 2 4.6 Scara5 Scavenger receptor class A 5 4.4 Hgsnat Heparan N-acetyltransferase 4.4 Hs3st1 Heparan sulphate 3-O-sulphotransferase 1 4.2 Syne1 Synaptic nuclear envelope 1 4.1 Slc38a5 Solute carrier family 38, member 5 4.0 Gpd1 Glycerol-3-phosphate dehydrogenase 1 3.9 Dos Downstream of Stk11 3.8 Svs5 Seminal vesicle secretory protein 5 3.8 Bhmt Betaine-homocysteine methyltransferase 3.7 D9Ertd280e Chr 9, ERATO Doi 280 3.7 Tnfrsf12a Tumour necrosis factor receptor 12a 3.7 1200016- RIKEN cDNA 1200016E24 E24Rik 3.7 Nr4a1 Nuclear receptor subfamily 4, group A1 . 3 6 Dkk3 Dickkopf homolog 3 . 3 6 Cyp51 Cytochrome P450, family 51 Transcripts up-regulated 24 h after start of treatment 28.8 Lin7c Lin-7 homolog C (C. elegans) 18.0 13.6 11.0 10.1 . 9 4 . 8 7 . 7 9 . 7 4 . 7 0 . 6 3 . 5 5 . 5 0 . 4 6 . 4 5 . 4 2 . 4 2 . 4 2 . 4 1 . 4 0 . 4 0 . 4 0 . 4 0 . 3 8 Cyp17a1 Cyp11a1 Fabp5 Rhox5 Star Slc38a5 Aqp5 Tubb3 Drd4 Tesc Lgals1 Spinlw1 Osr1 Fads2 Pappa Scara5 Pscdbp Plac8 Gpt2 Rps6ka2 Gpd1 Hdc Igf1 Cytochrome P450, family 17a1 Cytochrome P450, family 11a1 Fatty acid binding protein 5, epidermal Reproductive homeobox 5 Steroidogenic acute regulatory protein Solute carrier family 38, member 5 Aquaporin 5 Tubulin, b 3 Dopamine receptor 4 Tescalcin Lectin, galactose binding, soluble 1 Eppin Odd-skipped related 1 Fatty acid desaturase 2 Pregnancy-associated plasma protein A Scavenger receptor class A5 Pleckstrin homology binding protein Placenta-speciﬁc 8 Glutamic pyruvate transaminase 2 Ribosomal protein S6 kinase 2 Glycerol-3-phosphate dehydrogenase 1 Histidine decarboxylase Insulin-like growth factor 1 Transcripts down-regulated 12 h after start of treatment 7.27 Myh8 Myosin, heavy polypeptide 8 6.65 Rin2 Ras and Rab interactor 2 5.03 Pdgfc Platelet-derived growth factor C 4.55 Transcribed locusb 4.53 Brevican Bcan 4.46 Regulator of G-protein signalling 11 Rgs11 4.45 Transmembrane protein 37 Tmem37 4.43 Formin homology 2 domain containing 3 Fhod3 4.38 Der1-like domain family, member 3 Derl3 4.01 DNA-damage-inducible transcript 4-like Ddit4l 3.98 cDNA sequence BC013672 BC013672 3.97 Scinderin Scin 3.96 DNA-damage-inducible transcript 4-like Ddit4l 3.89 Chaperone, ABC1 complex-like Cabc1 3.86 Keratin 20 Krt20 3.74 Transmembrane protein 140 Tmem140 3.71 D site albumin promoter binding protein Dbp 3.71 Ribonuclease L Rnasel 3.70 splA receptor domain and SOCS box 1 Spsb1 3.67 Troponin I, cardiac Tnni3 3.65 Cysteine dioxygenase 1, cytosolic Cdo1 3.57 START domain containing 8 Stard8 3.55 Solute carrier family 40, member 1 Slc40a1 3.52 Histone deacetylase 5 Hdac5 3.32 D site albumin promoter binding protein Dbp 3.23 Choline dehydrogenase Chdh 3.23 RIKEN cDNA 8030411F24 gene 8030411- F24Rik 3.23 Per3 Period homolog 3 (Drosophila) 3.22 3.19 Ctnna2 Trim47 Catenin, a 2 Tripartite motif protein 47 Transcripts down-regulated 24 h after start of treatment 9.9 Ddit4l DNA-damage-inducible transcript 4-like 9.9 Rin2 Ras and Rab interactor 2 9.1 Slc40a1 Solute carrier family 40, member 1 8.0 Rgs11 Regulator of G-protein signalling 11 7.6 Igfbp3 Insulin-like growth factor binding protein 3 7.4 Myh6 Myosin, heavy polypeptide 6 a 7.2 Apbb2 Amyloid precursor protein-binding B2 6.7 Rassf5 Ras association domain family 5 6.3 Tmem37 Transmembrane protein 37 6.3 Chdh Choline dehydrogenase 6.2 Itga9 Integrin a 9 6.2 Thbd Thrombomodulin 5.9 Fcgr2b Fc receptor, IgG, low afﬁnity IIb 5.8 Trim47 Tripartite motif protein 47 5.7 Spsb1 splA receptor domain and SOCS box 1 5.6 Ddit4l DNA-damage-inducible transcript 4-like 5.5 Vnn1 Vanin 1 5.5 Fcgr2b Fc receptor, IgG, low afﬁnity IIb 5.4 Ptprd Protein tyrosine phosphatase, receptor D 5.4 Nkx3-1 NK-3 transcription factor, locus 1 5.3 Fcgr2b Fc receptor, IgG, low afﬁnity IIb 5.1 Ctgf Connective tissue growth factor 5.0 H19 H19 fetal liver mRNA 5.0 Pdgfc Platelet-derived growth factor C (continued) Journal of Molecular Endocrinology (2009) 42, 291–303 www.endocrinology-journals.org