Inflammatory Gene Expression in SGBS Adipocytes Table 3. Real-Time PCR Analysis of Up- and Down-Regulated Genes of TNF-α-treated, Differentiated SGBS Cells A) Up-regulated genes Name Average CT CT CT 2-ΔΔCT D20 VCAM 29.35 2.90 0 1 MCP-1 31.70 5.25 0 1 Eotaxin-1 32.65 6.20 0 1 CH25 29.00 2.55 0 1 TNFSF13B 30.45 4.00 0 1 RGS2 32.25 5.80 0 1 GAPDH 26.45 - - - D20//TNF-α VCAM1 27.45 1.10 -1.80 3.48 MCP-1 26.00 -0.35 -5.60 48.50 Eotaxin-1 30.10 3.75 -2.45 5.46 CH25 30.95 4.60 2.05 0.24 TNFSF13B 32.45 6.10 2.10 0.23 RGS2 32.90 6.55 0.75 0.59 GAPDH 26.35 - - - B) Down-regulated genes Name Average CT CT CT 2-ΔΔCT D20 IL1F5 30.80 8.05 0 1 ADAMTS 30.20 7.45 0 1 FN1 22.10 -0.65 0 1 MMP15 26.40 3.65 0 1 PTGES 26.45 3.70 0 1 GAPDH 22.75 - - - D20/TNF-α IL1F5 33.25 11.55 3.50 0.089 ADAMTS 30.00 8.30 0.85 0.550 FN1 21.95 0.25 0.90 0.500 MMP15 29.10 7.40 3.75 0.074 PTGES 22.65 0.95 -2.75 6.730 GAPDH 21.70 - - - CT, Avg.sample CT-Avg.GAPDH CT; CT, Avg. CT-Avg. CT, before; 2- CT, normalized sample (after) amount relative to sample (before). in cartilage treated with TNF-α, retinoic acid, and 45 kDa fibronectin fragments.16,17 It has also been reported that the expression of several MMPs is elevated in cartilage and synovial tissues of patients with rheumatoid arthritis and osteoar- thritis.18,19 There have been a few reports that ADAMTS1 and fibronectin are expressed in adipocytes, but their function in adipocytes is not Yonsei Med J Vol. 47, No. 5, 2006