© 2012 Landes Bioscience. Do not distribute. Figure 3. Five ReDs exhibit different tissue patterns of DNa methylation. DNa methylation in villi (n = 11), brain (n = 8), kidney (n = 11) and muscle (n = 10) from 2nd trimester fetuses and adult blood (n = 10) was measured using (a ) % a lu, (B) % LINE-1, (c ) strong island probes, (D) weak island probes and (E) non-island probes. LINE-1, weak island and non-island probe methylation were most variable tissue to tissue. Villi DNa methylation was significantly reduced compared with most other somatic tissues at LINE-1, weak island and non-island probes. h owever villi DNa methylation was significantly increased compared with kidney and muscle at strong island probes. s ignificance is indicated by *p < 0.05, **p < 0.001, ***p < 0.0001. were obtained from 12 fetuses and were thus used to investigate intra-individual DNA methylation. None of the ReDS was uni- versally significantly correlated across all four tissues analyzed within an individual (Fig. S12A–E). However, weak island probe methylation was significantly correlated in villi and kidney (Fig. S12D; r = 0.70, p = 0.03) and villi and brain (Fig. S12D; r = 0.89, p = 0.01). Additionally, Alu DNA methylation was cor- related in villi and kidney (Fig. S12A; r = 0.82, p = 0.01). Overall, we observed more positive than negative correlation coefficients (Fig. S12; 18 vs. 7). In particular, there were more comparisons www.landesbioscience.com Epigenetics 657