© 2012 Landes Bioscience. Do not distribute. Figure 5. Increase in DNa methylation at weak and non-islands throughout gestation. DNa methylation in 1st trimester (n = 10), 2nd trimester (n = 11) and term (n = 10) placental villi was measured with (a ) % a lu, (B) % LINE-1, (c ) strong island probes, (D) weak island probes and (E) non-island probes. There was no change in LINE-1 or strong island methylation, but a notable increase in methylation at weak and non-islands throughout gestation. s ignificance is indicated by *p < 0.05, **p < 0.001, ***p < 0.0001. www.landesbioscience.com Epigenetics 659