Gene.Accession Gene.Symbol Gene.Description ENSMUST00000112686 Cecr2 NM_013904 NM_019450 NM_181820 NM_001030274 NM_030087 NM_026626 Hey2 Il1f6 Tmc4 Ndufs5 Ndufv3 Efcab2 CHF1/Hey2 Regulates Hypertrophy Current Genomics, 2010, Vol. 11, No. 4 289 Table 1. Genes Demonstrating Significant Changes in Expression in CHF1/Hey2 Trangenic Myocytes vs Wild Type myocytes After Serum Stimulation EF-hand calcium binding domain 2 0.473221857 6.46E-05 0.12082322 P.Value 2.73E-07 1.98E-05 2.26E-05 2.31E-05 3.95E-05 FDR 0.009724348 0.117403674 0.117403674 0.117403674 0.12082322 0.347003714 6.16E-05 0.12082322 Hairy/enhancer-of-split related with YRPW motif 2 Interleukin 1 family, member 6 Cat eye syndrome chromosome region, candidate 2 homolog (human) NADH dehydrogenase (ubiquinone) Fe-S protein 5 NADH dehydrognase (ubiquinone) flavoprotein 3 Transmembrane channel-like gene family 4 -0.306575857 logFC 1.814541571 -0.429964571 0.437622286 0.341531429 Fig. (2). qRT-PCR of transcripts identified by microarray single gene analysis validates their expression profiles. RNA was harvested from multiple independent preparations of WT and TG cells treated with serum, reverse transcribed and amplified by standard qRT-PCR methods. Expression was normalized to GAPDH and compared by the 2- CT method as described [19].