992 Moreover, DNA adsorbed onto DNA-PLA-PEG-NPs was efﬁciently protected from nuclease degradation and could remain relatively stable in plasma. It was less toxic than commercial Lipofectamine 2000 and safer to Hela cell. And it could successfully transfer plasmid EGFP into Hela cells, what’s more, the transfection activity was not diminished dramatically by the serum in transfection medium which surpassed the commercial cationic lipo- some based reagent, Lipofectamine 2000. It could be anticipated that the established DNA-PLA-PEG-NPs in current study was a promising nonviral gene delivery system used in gene therapy. It has a potential to alleviate the acute toxicity effect of other vectors and prolong the circulation time in vivo. Our further study will focus on the optimization of the formulation for higher transfection efﬁciency and the in vivo performance of the DNA-PLA- PEG-NPs as gene delivery system will also be conducted. 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