Yu et al. Journal of Experimental & Clinical Cancer Research 2010, 29:107 http://www.jeccr.com/content/29/1/107 Page 7 of 7 (+)BAD(-)ones. The breast cancer cells in which BCL-2 and BAD are all positive are more chemosensitive to NVB than the BCL-2(+)BAD(-)ones(P < 0.05). We indi- cated that the union examination of BCL-2 and Bad might play a guiding role in the selection of chemother- apy drugs. Studies [11] confirmed that antisense oligo- nucleotide of BCL-2 can effectively reduce the expression of BCL-2 in breast cancer cells, reduced the inhibition caused by the BCL-2 gene in chemotherapy- induced apoptosis, improved the treatment effect. Anti- sense oligonucleotide of BCL-2 as an enhancer of the chemotherapeutic effect, provided us a new way for the treatment of breast cancer. Acknowledgements This work was supported by the Science and Technique Plan Projects of Chongqing Municipality.(CSTC 2008AC5082) Authors’ contributions BY did the immunohistochemical assay, XS: preformed the statistical analysis, and participated in culturing cell in vitro, HYS culturing the cell in vitro, FG did the MTT test, YMF colleted the sample, ZJS designed the experiment, analysed the data, and wrote this paper. All authors read and approved the final manuscript. Competing interests The authors declare that they have no competing interests. Received: 15 June 2010 Accepted: 7 August 2010 Published: 7 August 2010 References 1. Shimizu M, Saitoh Y, Itoh H: Immunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues. [J] Hum Pathol 1990, 21(6):607-612. 2. Mounia Chami, Andrea Prandini: Bcl-2 and Bax Exert Opposing Effects on Ca2+ Signaling, Which Do Not Depend on Their Putative Pore-forming Region. [J] Biol Chem 2004, 279(52):54581-54589. 3. Datta SR, Katsov A, Hu L, Petros A, Fesik SW, Yaffe MB, Greenberg ME: 14-3- 3 proteins and survival kinases cooperate to inactivate BAD by BH3 domain phosphorylation. [J] Mol Cell 2000, 6:41-51. 4. Kim R, Emi M: Therapeutic potential of antisense Bcl-2 as a chemosensitizer for patients with gastric carcinoma. Journal of Clinical Oncology 2005, 23(16S(June 1 Supplement)):4050. 5. Molto Luis, Rayman Pat: The Bcl-2 Transgene Protects T Cells from Renal Cell Carcinoma-mediated Apoptosis. Clinical Cancer Research 2003, 9:4060-4068. 6. Agrup M, Stal O, Olsen K, Wingren S: C-erbB-2 overexpression and survival in early onset breast cancer. [J] Breast Cancer Res Treat 2000, 63(1):23-29. 7. Milella M, Trisciuoglio Daniela, Bruno Tiziana: Trastuzumab Down-Regulates Bcl-2 Expression and Potentiates Apoptosis Induction by Bcl-2/Bcl-XL Bispecific Antisense Oligonucleotides in HER-2 Gene-Amplified Breast Cancer Cells. [J] Clinical Cancer Research 2004, 10:7747-7756. 8. Chami M, Prandini A, Campanella M, Pinton P, Szabadkai G, Reed JC, Rizzuto R: Bcl-2 and Bax exert opposing effect on Ca2+ signaling, which do not depend on their putative pore-forming region. [J] J Biol Chem 2004, 279(52):54581. 9. Linjawi A, Kontogiannea M, Halwani F, Edwardes M, Meterissian S: Prognostic significance of p53, Bcl-2, and Bax expression in early breast cancer. [J] Am Coll Surg 2004, 198(1):83. 10. Xiao-wei Wang, Bing-lin Guo, Zhong-hua Shang: Bcl-2 and Bad protein expression in breast carcinoma. [J] Chin J Gen Surg 2004, 19(9):564. 11. Tanabe K, Kim R, Inoue H, Emi M, Uchida Y, Toge T: Ant3. isense Bcl-2 and HER-2 oligonucleotide treatment of breast cancer cells enhances their sensitivity to anticancer drugs. [J] Int J Oncol 2003, 22:875-81. doi:10.1186/1756-9966-29-107 Cite this article as: Yu et al.: Expression of the apoptosis-related genes BCL-2 and BAD in human breast carcinoma and their associated relationship with chemosensitivity. Journal of Experimental & Clinical Cancer Research 2010 29:107. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit