802 Genomic detection of melanoma, W. Wachsman et al. Fig 2. Ingenuity Pathway Analysis of epidermal genetic information retrieval-harvested melanoma specimens identiﬁes overexpressed genes involved in melanocyte development and pigmentation. Melanocyte development and pigmentation is primarily regulated by microphthalmia- associated transcription factor (MITF), which is controlled mainly through the melanocyte-stimulating hormone (MSH) signalling pathway that includes adenylate cyclase 2 (ADCY2), sex determining region Y-box 10 (SOX10) and paired box 3 (PAX3) as well as the v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog (Kit) signalling pathway. Tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1) and dopachrome tautomerase (DCT) are activated by MITF and are also involved in skin pigmentation. under the curve (AUC) of 0Æ955, indicating that it is extremely accurate for melanoma detection. We used the 17-gene classiﬁer to evaluate several types of EGIR-harvested additional control lesions, including 73 normal, nonlesional skin samples from both healthy subjects (n = 53) and patients with mela- noma (n = 19) or basal cell carcinoma (n = 1), 18 pigmented basal cell carcinomas, and 22 solar lentigines. The assay for melanoma of these specimens was negative for all of the nonle- sional skin specimens and the solar lentigines, as well as 17 of the 18 basal cell carcinomas (data not shown). These ﬁndings provide further support for the speciﬁcity of the 17-gene classi- ﬁer to detect melanoma speciﬁcally. BJD 2011 The Authors 2011 British Association of Dermatologists 2011 164, pp797–806