1416 Sample Rice husk Sugarcane bagasse Coffee husk Table 3 Percent crystallization in particles SiO2 synthesized with bioprocess and without bioprocess Crystallization (%) With bioprocess 500 C 28.47 10.42 15.73 700 C 16.69 9.190 25.82 500 C 0.000 76.12 58.28 In this table is presented an estimative obtained for low curve areas in the peaks of XRD. These percents shows the contribution than the temperature of calcination and the bioprocess have on the crystalli- zation of nanoparticles of SiO2 nanoparticles using annelids’ biotransformation by employing agro-industrial wastes. The approach represents an inexpensive and relatively eco-friendly technology in comparison with standard chemical methods. By taking into account the biological aspects of the production of SiO2 nanoparticles with speciﬁc crystal arrangement using annelids allows to extend the number of living organism dedicate to biosiliﬁcation, in addition to open an interesting ﬁeld toward the knowledge of new annelid bioprocesses with a primitive metabolism, as potential alternative natural nanotechnology bioprocesses to synthesize nanoparticles and nanostructures, for the particles obtained through bio- transformation by annelids show similar characteristics than synthetic SiO2 Aerosil 130 such as size, composition and polydispersity. While synthetic particles possess amorphous structure the particles synthesized using vermicompost present different disables characteristics such as crystalline (different polymorphism) and nanometric dimension. Acknowledgments The authors are grateful to Ms. Maria de Lourdes Palma for her assistance in TEM, to Dr. Genoveva Herna´ndez-Padron for her assistance in IR analysis, to Dr. Eric Rivera for his assistance in XRD, to CINVESTAV Quere´taro, particularly to Dr. S. Jimenez and Mr. F Rodriguez for their assistance in some measurements and to DGEST and Consejo Nacional de Ciencia y Tecnologia (CONACyT), Mexico, for the economical support through the projects P333-05 and JI-58232, respectively. Financial support from the National Council for Science and Technology of Mexico (CONACYT) (PhD Scholarship to A.E.-G.) is gratefully acknowledged. Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which per- mits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. Without bioprocess 700 C 1.910 13.71 17.36 Nanoscale Res Lett (2010) 5:1408–1417 2. E.D. 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