The Journal of Comparative Neurology SINGLE CELL TRANSCRIPTIONAL PROFILE OF MGCS 235 Fig. 6. Dissociated cell ISH conﬁrms expression in M u¨ller glia. A–J: Retinae from mature mice were dissociated, plated on slides, and processed for detection by ISH for the indicated probes (ApoE, Dkk3, Apg4b, Itm2b, Rlbp1). Merged images show DAPI stain and immuno-staining for Glul and ISH. Insets are digital magniﬁcations. Scale bar 25 m in A (applies to A,B), C (ap- plies to C,D), E (applies to E,F), G (applies to G,H), and I (applies to I,J). line will provide genetic access for manipulations of Mu¨ ller glia in mice, in addition to the recently published lentiviral vector-based approach applied in rats by Greenberg et al. (2007). The utility of such methods might be especially interesting in retinal disease back- grounds. Relationship of M u¨ller glia to progenitor cells and stem cells A SAGE study, coupled with large-scale in situ hybrid- izations, showed that genes enriched or speciﬁc to M u¨ller glia in the mature retina are also expressed in multipotent