Inflammatory Gene Expression in SGBS Adipocytes B D B A C A DISCUSSION The present study shows that treatment of dif- ferentiated SGBS cells with the pleiotropic pro- inflammatory cytokine, TNF-α, increased expres- Fig. 1. Oil Red O staining of SGBS cells at (A) Day 0 and at (B) Day 4, (C) Day 10, and (D) Day 20 after differentiation. The stained lipid droplets are shown in red. Fig. 2. Scanned image data of a MWG human inflammatory array, hybrid- ized with cRNA prepared from dif- ferentiated SGBS (Day 20) and TNF-α- treated differentiated SGBS cells (Day 20/TNF-α). (A) Day 20 cells are Cy3- labeled and Day 20/TNF-α cells are Cy5-labeled. (B) Day 20 cells are Cy5- labeled and Day 20/TNF-α cells are Cy3-labeled. sion of six, and decreased the expression of five, inflammation-related genes. Real-time PCR an- alysis did not always confirm the results from array analysis, and reason for this discrepancy may be the cross-hybridization of oligonucleotides Yonsei Med J Vol. 47, No. 5, 2006