254 Cerebellum (2012) 11:246–258 Fig. 5 Silver impregnation of cerebellar sections in Cacna1a Purkinje cell-specific knockout mice. a, d No apparent neurodegeneration is seen in the cerebellum of PCα1KO mice at P30. b, e Silver-stained Purkinje cell bodies as well as dendrites and axons indicating dying cells in the cerebellum of PCα1KO mice at P45. Arrows indicate dying Purkinje cells in (e). c, f No Purkinje cells are present the respective areas. Silver-stained cells can be found in the granular and molecular layers indicating some granule and interneuron loss at P200–250. G granule cell layer, PC Purkinje cell layer, M molecular layer, WM white matter. Roman numbers indicate the cerebellar lobules. Dotted boxes in (a–c) correspond to regions shown in (d–f) Fig. 6 Silver impregnation of cerebellar section reveal stained axons surrounding the medial cerebellar nuclei in Cacna1a Purkinje cell-specific knockout mice. a–d Silver-stained Purkinje cells and their axons are found in PCα1KO mice at P60 (c, d) but not in tissue from WT littermates (a, b). Dotted boxes in (a, c) correspond to regions shown in (b, d)