n Female/male Median age (range) Diagnose AML NOS t-myeloid neoplasm AML with MLD MDS RA RARS RCMD RAEB-1 and RAEB-2 del (5q): Unclassiﬁable MPN CML-AP PMF PV MDS/MPN RARS-T CMML: Unclassiﬁable Others Hemoglobin, g/l MCV, fL MCH, pg MCHC, g/dl RDW, % hypoRBC,% Retic (·109/l) CHr, pg Erythrogram pattern RS-pattern Any other pattern 15% of RS in BM With RS 38 18/20 67.5 (23–95) 6 3 – 3 21 – 6 9 6 – – 3 2 1 – 6 4 2 – 2 95 (61–151) 91.2 (75.4–113.7) 30.8 (22.8–37) 335.5 (265–365) 21.4 (14.6–33.8) 5.3 (0.3–78) 73.6 (9–251) 33 (25–51) 32 6 38 Without RS 30 9/21 64 (23–91) 6 – – 6 21 3 – 3 12 1 2 – – – – 3 – 1 2 – 95 (68–144) 99.7 (84–116.7) 34.7 (27.6–39.9) 340.5 (307–367) 17.1 (13.8–28.6) 1.3 (0.1–21.2) 39.4 (0.5–164.9) 37 (28–41) 0 30 0 0.72 0.001 0.0002 0.203 0.0002 0.00006 0.002 0.002 <0.000001 Validation 21 9/12 63 (42–85) 4 3 1 – 11 – 8 – 3 – – 4 2 1 1 1 – – 1 1 95.5 (80–121) 93.6 (78.1–100.7) 31.5 (26.2–36.3) 336 (300–346) 22.6 (19–28) 6.7 (3.3–20.9) 49.9 (5.4–209.6) 33.5 (27–38) 21 0 20 0.98 0.53 0.64 0.72 0.019 0.25 0.14 0.67 332 A. ROVÓ ET AL. ERYTHROGRAM PATTERN OF SIDEROBLASTIC CHANGES Table 1. Patient’s characteristics and RBCs values. In the ﬁrst step analysis, comparison of patients with and without RS; in the second step analysis, validation with patients presenting a typical RS-pattern Analysis of the ﬁrst step Second validation step P-value (with RS vs. without RS) P-value (with RS vs. validation group) RS, ringed sideroblasts; AML, acute myeloid leukemia; NOS, not otherwise speciﬁed; t-myeloid neoplasm, therapy- related myeloid neoplasm; AML with MLD, AML with multilineage dysplasia; MDS, myelodysplastic syndromes; RA, refractory anemia; RARS, refractory anemia with ring sideroblasts; RCMD, refractory cytopenia with multilineage dysplasia; RAEB-1 and RAEB-2, refractory anemia with excess blasts 1 and 2; del (5q), associated with isolated del (5q); MPN, myeloproliferative neoplasms; CML-AP, CML BCR-ABL1 positive in accelerated phase; PMF, primary myeloﬁbrosis; PV, polycythemia vera; RARS-T, RARS associated with marked thrombocytosis; CMML, chronic myelo- monocytic leukemia; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RDW, red cell distribution width; hypoRBC, hypochromic erythrocyte; Retic, absolute reticulocytes; CHr, content of hemoglobin of the reticulocytes; BM, bone marrow cytology. 2009 The Authors Journal compilation 2009 Blackwell Publishing Ltd, Int. Jnl. Lab. Hem. 2010, 32, 329–335