FTY720 treatment in skin allograft rejection Commodaro AG et al. CLINICS 2012;67(7):805-813 Figure 3 - Evaluation of graft-infiltrating cells five days after F1 skin graft transplantation in the C57BL/6 mice. A - A representative flow cytometry dot plot showing the graft-infiltrating cells (FSC x SSC) and Treg cells (CD4+Foxp3+). B - FTY720 treatment reduces the number of graft-infiltrating cells in the F1 transplanted skin (p = 0.02). C - FTY720 treatment reduces the percentage of CD4+ (p = 0.014) graft-infiltrating cells in the F1 transplanted skin. D - FTY720 reduces but does not prevent the accumulation of Foxp3+ graft-infiltrating cells in the F1 transplanted skin (p = 0.011). The results are expressed as the means ¡ SD. Statistical analysis The skin allograft survival curve was obtained using the Kaplan-Meier method. Other data were expressed as the means¡SD. The statistical analyses were performed using Graph Pad Prism version 5.00 for Windows (Graph Pad Soft- ware, San Diego, CA, USA). The parametric Student’s t test was employed, and p-values ,0.05 were considered significant. RESULTS Treatment with FTY720 increases skin allograft survival To evaluate the effect of FTY720 on the progression of skin allograft rejection, the recipient mice were either transplanted only (Tx, n = 5) or transplanted and submitted to the daily 808