Young Tae Kim and Min Zhao Table 1. Expression Status of p16 in Squamous Cervical Carcinoma Reference No. Pathology No. of cases Expression status Technique 8. Murphy et al. cGIN 12 100% positive expression Immuno-staining CA 11 100% positive expression 9. Kim et al. SCC 20 Mutations in exon2 PCR-SSCP 10. Dong et al. SCC 31 39% methylation MSP CA 22 18% methylation 12. Tsuda et al. CIN 42 14.3% positive expression Immuno-staining IC 53 43.4% positive expression 13. Hirama et al. SCC 41 No deletions or mutations Southern blot/SSCP CC cell line 8 No deletions or mutations 15. Sano et al. SCC/CIS 34 100% strong positive Immuno-staining GCA 16 100% positive expression DC 15 100% positive expression 16. Klaes et al. CINI 47 100% overexpressiom Immuno-staining 7 (LR-HPV) No expression CINII 32 100% overexpressiom CINIII 60 100% overexpressiom IC 60 97% overexpressiom 18. Volgareva et al. CINI 51 37.3% positive expression Immuno-staining CINII 38 31.6% positive expression CINIII 24 66.7% positive expression IC 21 95.2% positive expression AC 5 100% positive expression 21. Murphy et al. cGIN 5 100% positive expression Immuno-staining CINI 33 100% positive expression CINII 38 100% positive expression CINIII 46 98% positive expression IC 10 100% positive expression 22. Guo et al. LSIL 107 57.9% positive expression Immuno-staining HSIL CIN2/3 103 97.1% positive expression 23. Saqi et al. LSIL 27 74% positive expression Immuno-staining HSIL 10 90% positive expression SCC 1 100% positive expression 24. Hu et al. CINI 45 44% positive expression Immuno-staining CINII 46 93% positive expression CINIII 51 100% positive expression cGIN, cervical glandular intraepithelial neoplasia; CA, Cervical adenocarcinoma; SCC, squamous cell carcinoma; CIN, cervical intrae- pithelial neoplasia; LSIL, low-grade squamous intraepithelial; HSIL, high-grade squamous intraepithelial; IC, invasive carcinoma; CIS, carcinomas or carcinoma in situ; GCA, genital condyloma acuminate; DC, dysplasia of the cervix; LR-HPV, low-risk HPV; MSP, methylation-specific PCR; PCR-SSCP, polymerase chain reaction-single strand conformation polymorphism. invasive cervical cancer that were not associated with increased protein amounts. Additionally, un- derexpression of cyclin D1 was shown to be regulated at the transcriptional level in another Yonsei Med J Vol. 46, No. 5, 2005