Int. J. Environ. Res. Public Health 2012, 9 907 Figure 6. Phylogenetic tree based on the partial nucleotide sequences of ORF2 (190 bp) of HAstV strains. The strains of the present study are in bold face. Scale indicates genetic distance. 3.4.6. EV 3.4.7. AiV Of the 36 [sixteen (March) and twenty (October)] EV strains amplified for 5′ NCR, only 19 [52.8%, seven (March) and twelve (October)] were genotyped (Table 3). Phylogenetic analysis of the sequences obtained on the basis of VP1/2A region (450 bp) for 17/19 strains revealed the presence of EV-76 in two, EV-84 in one, EV-89 in one, EV-90 in four, CA-13 in one, CA-17 in one, CA-19 in one, CA-19/22 in one, CA-21 in two, Echo-21 in one and Echo-32 in two strains and indicated 81.4– 98.0% nucleotide identities with the corresponding reference strains (Figure 7). The remaining two strains were classified as EV-76 on the basis of VP1 gene sequences (data not shown). Phylogenetic analysis of the sequences obtained for 3C-3D junction region (265 bp) classified all of the 4 [one (March) and three (October)] AiV strains in genotype B and showed 93.7–98.9% nucleotide identity with B-171/05 (EF079157) and QId/2008/204 (EU715251) strains from Bangladesh and Australia, respectively (Figure 8, Table 3 ).