Kosger, Ozturk, Sokmen, Bulut, Ay The genus Arnebia (Boraginaceae) are carry out the experiment. Forty-eight animals represented by 4 species in the flora of Turkey, were mainly divided to two groups (scalpel with one of which, Arnebia densiolfra (Nordm.) Ledeb. is and without extract). Each main group was divided widespread in Sivas district2 and known as egnik to four subgroup containing six rats in each to by local people and used as red colouring for observe changes after 4th, 7th, 14th, and 21st days. dying the carpets and the rugs.5 Also, A. densioflra Animals were housed in metal cages and provided roots soaked in butter are used in local wound with standard food and tap water ad libitum. healing care. The roots of this plant have been reported to contain alkannin derivatives, namely Incision wound β,β-dimethylacrylalkannin, teracrylalkannin and All animals were anaesthetized intramuscularly isovalerylalkannin + α-methyl-n-butylalkannin.6 with ketamine plus xylazin combination. A 10-mm This study was designed to explore the healing length full-thickness incision wound was made in effects of topically applied ointment prepared from the mucoperiosteum of midline of the hard palate A. densiolfra root extracts in rat intraoral wound. using number 15 scalpel. No medication was used throughout the experiment. After the incision was MAtERIALS ANd MEtHodS made, incised mucosa sutured with single cat gut Collection of plant material sutures. The ointment was applied to the wound A. densiolfra plants (Boraginaceae) were once a daily in the experimental group animals. collected from the Ulas, Sivas, Turkey in June. Animals were sacrificed in 4th, 7th, 14th, 21st days. It was identified by Dr. Erol Donmez at the Histopathological examinations Department of Biology, Cumhuriyet University, After the creation of the wound, the rats Turkey. Voucher specimens have been deposited were sacrificed at 4th, 7th, 14th or 21st days and at the Herbarium of the Department of Biology, the wound area excised. The tissue was fixed in Cumhuriyet University, Turkey. 10% neutral formalin solution. The formalin-fixed Preparation of the n-hexane extract tissues were dehydrated, embedded in paraffin. The air-dried and powdered roots of A. The 5-7 µm sections of the tissues were stained densiolfra were extracted with n-hexane using with Haematoxylin and Eosin or Mallory Azan, and Soxhlet extraction apparatus for 12 hours. The evaluated for the histological changes under light extract was concentrated under reduced pressure microscope (Jenamed II, Carl Zeiss, Gottingen, (yield 5.3% w/w). The ointment was prepared as Germany). 10% (w/w) concentration, e.g. 5 g of extract was RESuLtS incorporated in 45 g of ointment base (lanolin and liquid paraffin). Findings on each group were evaluated and histological differences were compared between Animals control and experimental groups of section. Wistar albino rats (200-220 gr) were used to At the 4th day, no healing was observed in the Figure 1. Illustrates the day 4 untreated (a) and A. densiflora treated (b) incisional (I) regions made by scalpel. Epithelium (E), subepithelium (S), a: MAx20, b: HEx10. April 2009 - Vol.3 97 European Journal of Dentistry