Zhang et al. Journal of Experimental & Clinical Cancer Research 2010, 29:52 http://www.jeccr.com/content/29/1/52 Page 7 of 7 Authors' contributions JFZ carried out most of the experiments and organized data for manuscript. FW, HPW, HML, XFC performed some experiments involving in viral construc- tion, package, Western blot or cell culture. WQ and PKR participated in data organization and manuscript drafting. QH performed project design and man- uscript writing. All authors read and approved the final manuscript. Acknowledgements This work was supported by Grant of National Basic Research Project of China (2010CB529902), National High-tech R&D program (2007AA021202), National Natural Science Foundation for Outstanding Youth (30325043). Author Details 1Central Experimental Laboratory, First People's Hospital, Shanghai Jiaotong University, 85 Wujin Road, Shanghai 200080, PR China and 2School of Life Science, Guangdong Pharmaceutical University, Guangzhou 510006, PR China Received: 20 December 2009 Accepted: 20 May 2010 Published: 20 May 2010 JT©oh2uis0rnia1sr0taliZnoclhfeOEaipxnseganveriaAtmiclae;nbetlsiecalfan&rostCmeiceil:nhBcditoaspMltC:r/eiabdwnucCwtewrdnR.tjuerasnceldaLre.tcrdot.hm2e0/ct1eo0rn,mt2es9no:5tf/2th9e/1C/5re2ativeCommonsAt ributionLicense(ht p:/ creativecommons.org/licenses/by/2.0),whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. References 1. Lee CB, Stinchcombe TE, Rosenman JG, Socinski MA: Therapeutic advances in local-regional therapy for stage III non-small-cell lung cancer: evolving role of dose-escalated conformal (3-dimensional) radiation therapy. Clin Lung Cancer 2006, 8:195-202. 2. Rossi A, Maione P, Colantuoni G, Ferrara C, Rossi E, Guerriero C, Nicolella D, Falanga M, Palazzolo G, Gridelli C: Recent developments of targeted therapies in the treatment of non-small cell lung cancer. Curr Drug Discov Technol 2009, 6:91-102. 3. Ricciardi S, Tomao S, Marinis F: Toxicity of targeted therapy in non-small- cell lung cancer management. Clin Lung Cancer 2009, 10:28-35. 4. Herbst RS, Sandler AB: Overview of the current status of human epidermal growth factor receptor inhibitors in lung cancer. Clin Lung Cancer 2004, 6:S7-S19. 5. Baumann M, Krause M, Zips D, Petersen C, Dittmann K, Dörr W, Rodemann HP: Molecular targeting in radiotherapy of lung cancer. Lung Cancer 2004, 45:S187-197. 6. Määttä AM, Tenhunen A, Pasanen T, Meriläinen O, Pellinen R, Mäkinen K, Alhava E, Wahlfors J: Non-small cell lung cancer as a target disease for herpes simplex type 1 thymidine kinase-ganciclovir gene therapy. Int J Oncol 2004, 24:943-949. 7. Nemunaitis J, Vorhies JS, Pappen B, Senzer N: 10-year follow-up of gene- modified adenoviral-based therapy in 146 non-small-cell lung cancer patients. Cancer Gene Ther 2007, 14:762-763. 8. Lee SJ, Zhang Y, Lee SD, Jung C, Li X, Kim HS, Bae KH, Jeng MH, Kao C, Gardner T: Targeting prostate cancer with conditionally replicative adenovirus using PSMA enhancer. Mol Ther 2004, 10:1051-1058. 9. Ulasov IV, Zhu ZB, Tyler MA, Han Y, Rivera AA, Khramtsov A, Curiel DT, Lesniak MS: Survivin-driven and fiber-modified oncolytic adenovirus exhibits potent antitumor activity in established intracranial glioma. Hum Gene Ther 2007, 18:589-602. 10. Strazisar M, Mlakar V, Glavac D: The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC). Cell Mol Biol Lett 2009, 14:442-4569. 11. Ji X, Zhang J, Cheng L, Wei F, Li H, Liu X, Chen X, Li C, Wang Y, Huang Q: Oncolytic adenovirus delivering herpes simplex virus thymidine kinase suicide gene reduces the growth of human retinoblastoma in an in vivo mouse model. Exp Eye Res 2009, 89:193-199. 12. Huang Q, Zhang X, Wang H, Yan B, Kirkpatrick J, Dewhrist MW, Li CY: A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells. Clin Cancer Res 2004, 10:1439-1445. doi: 10.1186/1756-9966-29-52 Cite this article as: Zhang et al., Potent anti-tumor activity of telomerase- dependent and HSV-TK armed oncolytic adenovirus for non-small cell lung cancer in vitro and in vivo Journal of Experimental & Clinical Cancer Research 2010, 29:52