Plasmodium falciparum Hsp70–Hsp90 organising protein 199 Fig. 4 Localisation and expression of PfHop-GFP by parasites in human red blood cells. a Localisation of PfHop in P. falciparum cells; panels show a DIC image, nuclear stain (Hoechst), distribution of PfHop, merge and overlay. b Panels show a DIC image, nuclear stain (Hoechst), distribution of PfHop-GFP, merge and overlay. c Panels show a DIC image, nucleus (Hoechst), distribution of PfHop-GFP, distribution of PfHsp90, merge and overlay for PfHsp90–PfHop-GFP co-localisation. d Panels show DIC image, stain (Hoechst), distribu- tion of PfHop-GFP, distribution of PfHsp70, merge and overlay. e Western blot analyses to confirm the expression of PfHop-GFP. First panel, Western blot conducted using polyclonal anti-PfHop anti- bodies; second panel, Western blot conducted using monoclonal anti- GFP antibodies; and third panel, Western blot conducted as loading control conducted using polyclonal anti-PfAldolase antibodies. The results are representative of at least three independent experiments