Wen-Li et al. Journal of Experimental & Clinical Cancer Research 2012, 31:92 http://www.jeccr.com/content/31/1/92 Page 7 of 9 Figure 4 Analysis of effect of McAb7E10 on the cell cycle in AML cell lines. Cells were harvested, fixed, stained with propidium iodide staining and analyzed by flow cytometry. (A) Cell cycle analysis results of MV4-11 and HL-60 cell treated with different dose of McAb7E10. (B) The relative rate of apoptosis in 5, 10 and 50 ug/mL McAb7E10 treated MV4-11 cells was 3.6 ± 0.83%, 8.4 ± 1.69% and 17.3 ± 2.56% compared to 1.5% ± 0.85% in mouse IgG treated cells, p < 0.01. (C) The relative rate of apoptosis in 5, 10 and 50 ug/mL McAb7E10 treated HL-60 cells was 5.5 ± 2.37%, 11.3 ± 3.62% and 19.9 ± 3.31% compared to 1.56% ± 0.97% in mouse IgG treated cells, p < 0.01. Figure 5 McAb7E10 induces apoptosis in AML cell lines. (A, B) Annexin V staining and flow cytometry was used to confirm that McAb7E10 induced apoptosis in AML cells. (C) The relative rate of apoptosis in 50 μg/ml McAb7E10 treated MV4-11 cells was 50.5% ± 7.04% vs 21.9% ± 3.11% in mouse IgG treated cells, p < 0.01. (D) The relative rate of apoptosis in 50 μg/ml McAb7E10 treated HL-60 cells was 32.9% ± 4.52% vs 15.3% ± 3.95% in mouse IgG treated cells, p < 0.01.