Int. J. Environ. Res. Public Health 2010, 7 3228 suspension was filtered through sterile muslin and the filtrate was brought up to 100 mL with SROW. Streptomycin was added to the filtrate at the rate of 100 mg L−1 to inhibit bacteria, and decimal dilution series to 10−3 were prepared from the filtrate using SROW. An aliquot of 100 µL of each dilution was spread onto malt extract agar amended with chloramphenicol (MEAC) (malt extract 10 g, glucose 10 g, bacteriological agar 10 g, peptone 0.5 g, reverse osmosis water 500 mL, chloramphenicol 50 mg) in standard plastic Petri dishes. The culture plates were surface-dried in a biological safety cabinet for 1 h immediately before use. The spread plates were incubated at 25 °C in the dark. Yeast and filamentous fungal colonies were counted over 7 days and the estimated number of CFU g−1 of frog excrement was calculated. Filamentous colonies were identified to genus level and the estimated percentage of each genus recovered was calculated. This procedure was repeated on another five fresh faecal pellets of L. caerulea collected in September/October 2009 after several months of extremely dry, low-humidity weather. Ten yeast colonies, which appeared to be different to each other in terms of gross colony morphologies and which were represented in all of the samples, were picked from the cultures and sub-cultured. These were sent to Sullivan Nicolaides Pathology Laboratories in Rockhampton, Queensland for identification. 2.4. Skin Swabs—Yeast An area of 9 cm2 of the dorsal skin of each of 10 sleeping adult specimens of L. caerulea (CQUniversity Animal Ethics permit No. A10/04-258) was gently swabbed to determine if yeasts were endemic on the skin of these animals. A sterile cotton bud, with long wooden handle, dipped in SROW was used for this purpose. The cotton bud was then placed in a sterile 30 mL polycarbonate screw-top tube (Sarstedt) containing 10 mL of SROW, the wooden handle of the cotton bud was broken off, and the tube was capped. Sterile rubber gloves were worn to prevent accidental cross-contamination between operator and frog. The tubes were transported back to the laboratory in pre-cooled containers and were processed immediately on arrival. Each tube was vortexed on a CSV90 Auto vortex mixer at setting 8 for 30 sec. and the swab was discarded after expressing excess solution by pressing it against the side of the tube. A decimal dilution series to 10−3 was prepared from the contents of each of the ten tubes using SROW. Spread plates were prepared using MEAC culture plates which had been surface-dried in a biological safety cabinet for 1 hr. immediately before use. An aliquot of 200 µL of each of the dilution series was pipetted onto these plates and spread with sterile plastic spreaders. The culture plates were incubated in the dark at 25 °C and yeast colonies were counted daily for 3 days. Ten yeast colonies, which appeared to be different to each other in terms of gross colony morphologies and which were represented in all of the samples, were picked from the cultures and sub-cultured. These were sent to Sullivan Nicolaides Pathology Laboratories in Rockhampton, Queensland for identification. 2.5. Photographs and Photomicrographs Photographs were taken with a Nikon Coolpix 995 digital camera. Photomicrographs were taken with an Olympus Colorview III digital camera mounted on a Leica DMLB light microscope.