436 Purinergic Signalling (2010) 6:429–445 Fig. 4 Effects of purinergic and glutamatergic antagonists on nucleotide-induced [Ca2+]i increases in cells of the anterior telencephalon. a Mean peak responses (±SEM), expressed as percent of control response to 2MeSATP (100 μM), of 186 OB cells and 80 PVZ cells (three slices of the anterior telencephalon) in control conditions (red and green columns on the left-hand side, respectively), after 10 min in bath solution with 200 μM suramin (light red and light green column, respectively), and after 10 min of washout with bath solution (red and green columns on the right-hand side, respectively). b Same representation as in a with ATP (100 μM) and suramin [200 μM; 90 OB cells and 72 PVZ cells (two and three slices of the anterior telencephalon, respectively)]. c Same representation as in a with 2MeSATP (10 μM) and PPADS [200 μM; 155 OB cells and 216 PVZ cells (two slices of the anterior telencephalon)]. d Same representation as in a with ATP (50 μM) and PPADS [200 μM; 242 OB cells and 262 PVZ cells (three slices of the anterior telencepha- lon)]. e The peak amplitudes of ATP-induced (100 μM) mean [Ca2+]i transients (±SEM) of all responsive cells (OB: n = 21, red traces; PVZ: n = 47, green traces) of an individual slice of the anterior telenceph- alon are not significantly attenuated by 50 μM CNQX and 200 μM APV (OB: light red trace; PVZ: light green trace). f Mean peak responses (±SEM), expressed as percent of control response to ATP (100 μM), of 287 OB cells and 360 PVZ cells (seven slices of the anterior telencephalon) in control conditions (red and green columns on the left-hand side, respectively), after 5 min in bath solution with 50 μM CNQX and 200 μM APV (light red and light green column, respectively), and after 10 min of washout with bath solution (red and green columns on the right-hand side, respectively). (*p < 0.05 and **p < 0.01; unpaired t test)