Bairami et al. : A Sensitive and Specific PCR Based Method … analysis using isolated DNAs from Enta- moeba histolytica, Giardia duodenalis and Blastocystis hominis and showed no banding pattern. Fig. 1: PCR products of human samples (1-5) and cattle samples (6-9) on 1% agarose gel. WM: 100 bp molecular weight marker; lane 1: sample 229; lane 2: sample 145; lane 3: sample 142; lane 4: sample 233; lane 5: sample 234; lane 6: sample 192; lane 7: sample 183; lane 8: sample 163; lane 9: sample 194; lane 10: human negative fecal sample; lane 11: positive control; lane 12: negative control (Distilled water instead of DNA template) Fig. 2: nested-PCR products of human samples (1-5) and cattle samples (6-9) on 1% agarose gel. WM: 100 bp molecular weight marker; lane 1: sample 229; lane 2: sample 145; lane 3: sample 142; lane 4: sample 233; lane 5: sample 234; lane 6: sample 192; lane 7: sample 183; lane 8: sam- ple 163; lane 9: sample 194; lane 10: human negative fecal sample; lane 11: positive control; lane 12: negative control (Distilled water instead of DNA template) 4