Journal of Proteome Research ARTICLE Figure 2. Characterization of the An. gambiae adult midgut DRM proteome. (a) Distribution of the total DRM proteins into categories based on intracellular location (N = 1,452) (see text and Table S1, Supporting Information, for details). (b) Distribution of the predicted extracellular DRM proteins into categories based on function (N = 191). (c) Spectral heat map representing a subset of extracellular proteins (N = 80) that were identiﬁed across the three biological DRM replicates (FDR < 1%), with the exception of AGAP003790. Included in this map are four out of the six the current transmission-blocking vaccine candidates listed in Table 1, as well as canonical lipid raft markers (see text and Table S4, Supporting Information, for details). Each row shows the row-normalized number of spectral identiﬁcations for each protein from each run (see scale bar). Normalization was carried out as described previously.16 proteins were annotated functionally according to conserved domains as predicted by VectorBase (VB-2011 04), InterPro, UniProt, and BLAST searching using the nonredundant protein sequences database on NCBI. The identiﬁed proteins were then assigned to various categories based on their predicted cellular localization (Figure 2a). Bioinformatics analysis predicted that a considerable number of these proteins are likely to be cyto- plasmic (23.1%) and cytoskeletal (5%). The presence of these proteins has been previously reported from biochemical and proteomic analyses of DRM preparations from many diﬀerent 5155 dx.doi.org/10.1021/pr2006268 |J. Proteome Res. 2011, 10, 5150–5162