In vivo analysis of traditional amalgam restorations CONCLUSION The restorative clinical procedure following hand caries removal, indirect pulp capping with GIC, varnishing and amalgam filling the cavity did not provide secondary caries and increased dentin mineral content after 12 months. REFERENCES 1. Dennison JB, Hamilton JC. Treatment decisions and con- servation of tooth structure. Dent Ciln N Am 2005;49:825- 845. 2. Silva NR, Carvalho RM, Pegoraro LF, Tay FR, Thompson VP. Evaluation of a self-limiting concept in dentinal caries re- moval. J Dent Res 2006;85:282-286. 3. ten Cate JM. Remineralization of caries lesions extending into dentin. J Dent Res 2001;80:1407-1411. 4. McComb D. Systematic review of conservative operative caries management strategies. J Dent Educ 2001;65:1154- 1161. 5. Kidd EA. How ‘clean’ must a cavity be before restoration? Caries Res 2004;38:305-313. 6. Sato Y, Fusayama T. Removal of dentin by fuchsin staining. J Dent Res 1976;55:678-683. 7. Krause F, Braun A, Eberhard J, Jepsen S. Laser fluores- cence measurements compared to electrical resistance of residual dentine in excavated cavities in vivo. Caries Res 2007;41:135-140. 8. Banerjee A, Kidd EA, Watson TF. In vitro validation of cari- ous dentin removed using different excavation criteria. Am J Dent 2003;16:228-230. 9. Smales RJ, Ngo HC, Yip KHK, Yu C. Clinical effects of glass ionomer restorations on residual carious dentin in primary molars. Am J Dent 2005;18:188-192. 10. Thylstrup A, Fejerskov O. Textbook of clinical cariology. Co- penhagen, Munksgaard 1994. 11. Willmott NS, Wong FS, Davis GR. An X-ray microtomogra- phy study on the mineral concentration of carious dentine removed during cavity preparation in deciduous molars. Car Res 2007;41:129-134. 12. Mertz-Fairhurst EJ, Curtis Jr. JW, Ergle JW, Rueggeberg FA, Adair SM. Ultraconservative and cariostatic sealed res- torations: results at year 10. J Am Dent Assoc 1998;129:55- 66. 13. Alves LS, Fontanella V, Damo AC, Ferreira de Oliveira E, Maltz M. Qualitative and quantitative radiographic as- sessment of sealed carious dentin: a 10-year prospective study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:135-141. 268 European Journal of Dentistry 14. Silveira AD, Borges BC, Almeida HV, Lima KC, Pinheiro IV. Progression of Non-Cavitated Lesions in Dentin through a Nonsurgical Approach: A Preliminary 12-Month Clinical Observation. Eur J Dent 2012;6:34-42. 15. Cenci MS, Piva E, Potrich F, Formolo E, Demarco FF, Pow- ers JM. Microleakage in bonded amalgam restorations us- ing different adhesive materials. Braz Dent J 2004;15:13-18. 16. Vermeesch G, Leloup G, Delmee M, Vreven J. Antibacterial activity of glass-ionomer cements, compomers and resin composites: relatioship between acidity and material set- ting phase. J Oral Rehabil 2005;32:368-374. 17. da Silva AF, Piva E, Demarco FF, Correr-Sobrinho L, Osin- ga PW. Microleakage in conventional and bonded amal- gam restorations: influence of cavity volume. Oper Dent 2006;31:377-383. 18. Thompson VP, Kaim JM. Nonsurgical treatment of incipient and hidden caries. Dent Clin N Am 2005;49:905-921. 19. Bjorndal L, Larsen T, Thylstrup A. A clinical and micro- biological study of deep carious lesions during stepwise excavation using long treatment intervals. Caries Res 1997;31:411-417. 20. Oliveira EF, Carminatti G, Fontanella V, Maltz M. The moni- toring of deep caries lesions after incomplete dentine car- ies removal: results after 14-18 months. Clin Oral Invest 2006;10:134-139. 21. Hibst R, Paulus R, Lussi A. Detection of occlusal caries by laser luorescence. Basic and clinical investigations. Med Laser Appl 2001;16:205-213. 22. Chu CH, Lo EC, You DS. Clinical diagnosis of fissure caries with conventional and laser-induced fluorescence tech- niques. Lasers Med Sci 2010;25:355-362. 23. Kitasako Y, Nakajima M, Foxton RM, Aoki K, Pereira PN, Tagami J. Physiological remineralization of artificially de- mineralized dentin beneath glass ionomer cements with and without bacterial contamination in vivo. Oper Dent 2003;28:274-280. 24. ten Cate, van Duinen RN. Hypermineralization of dentinal lesions adjacent to glass-ionomer cement restorations. J Dent Res 1995;74:1266-1271. 25. Tantbirojn D, Feigal RJ, Ko CC, Versluis A. Remineralized dentin lesions induced by glass ionomer demonstrate in- creased resistance to subsequent acid challenge. Quintes- sence Int 2006;37:273-281. 26. Pinheiro IV, Borges BC, Colombo AP, Lima KC. Clinical and microbiological characteristics in predicting dentine caries progression. Acta Odontol Latinoam 2009;22:143-149. 27. Silveira AD, Borges BC, Almeida HV, Lima KC, Pinheiro IV. Progression of non-cavitated lesions in dentin through a nonsurgical approach: a preliminary 12-month clinical ob- servation. Eur J Dent 2011;5:229-236.