Journal of Proteome Research ARTICLE Figure 5. Overview of an enrichment analysis of Bm proteome classiﬁed with COGs and computational predictions categories. (A) Overview of Bm proteome classiﬁed with a total of 3291 COGs. The size of each slice represents the number of proteins assigned to each COG category. Proteins classiﬁed with COGs-U, M, N, O categories are signiﬁcantly overrepresented in serodominant antigens and shown in extracted red. COG-K proteins are signiﬁcantly underrepresented in serodominant antigens are shown in extracted black. COG categories with <5 proteins were not included in the ﬁgure (COGs-B, W). (B) Computational predictions for the Bm proteome are shown. Signiﬁcant over- and under-representations are shown in extracted red or black, respectively. Numbers of serodominant antigens within these categories/number of total proteins within these categories are in parentheses in the legend. Results of the complete COG analysis and computational predictions are in Table S3 (Supporting Information). (C) Correlation between enrichment fold and peptide numbers detected by Mass Spectrometry. As peptide numbers for serdiagnostic antigens increase, enrichment fold for serodiagnostic antigens also increases. In contrast, enrichment fold for cross-reactive antigens decreases as peptide numbers for cross-reactive antigens increase. yielded a high AUC of 0.97, with sensitivity rate of 96.7% and speciﬁcity rate of 96.7%. Comparing Blood Culture and Serological Testing by Agglutination Serological tests are routinely used to diagnose brucellosis. While the qualitative agglutination test (Rose Bengal) is ∼90% sensitive for screening for acute/subacute brucellosis,50,51 quantitative agglutination testing (e.g., tube agglutination test (TAT)) with titer typically greater than 1/160 used as a more precise diagnostic criterion, can also be used to diagnose relapse. Often blood cultures are negative at the time of initial presentation or relapse, possibly because of low levels of bacteremia or antecedent use of antibiotics. Without being able to ascertain whether the sera used in this study were obtained from patients who might have taken antibiotics prior to blood cultures, we queried our data set to 4818 dx.doi.org/10.1021/pr200619r |J. Proteome Res. 2011, 10, 4813–4824