TABLE 2 Comparative secretion of proteins identiﬁed from visceral and subcutaneous adipose tissue– derived preadipocytes Signal peptide? Functional description Visceral or subcutaneous WAT-derived preadipocytes were incubated in media containing different isotopes of arginine and lysine. Conditioned media were mixed, subjected to lectin afﬁnity chromatography, and subjected to quantitative mass spectrometry. Peak height ratios of isotope to endogenous (12C) amino acids and ratios between isotopes are shown. Complete dataset available in supplementary data. stromovascular fraction are able to differentiate into adi- pocytes and endothelial cells and vice versa (41). Anti- angiogenic agents have been proposed as novel therapeutic options in obesity as they induce weight loss in obese mouse models without adverse effect (42). In support of this hypothesis, cluster analysis of the 14 TABLE 3 Comparative secretion of proteins identiﬁed from visceral and subcutaneous adipose tissue– derived endothelial cells S.L. HOCKING AND ASSOCIATES Extracellular matrix Extracellular matrix Extracellular matrix Extracellular matrix Extracellular matrix Extracellular matrix Angiogenesis inhibitor Angiogenesis inhibitor, role in insulin resistance Extracellular matrix Haem binding protein, acute phase reactant Protease activity Inhibits C1s and C1r protease activity Serine protease inhibitor, inhibits matrix metalloproteinases, associated with insulin resistance Extracellular matrix Regulation of extracellular matrix Extracellular matrix Mechanical contraction Extracellular matrix Cysteine protease Regulation of growth and proliferation Extracellular matrix Growth factor binding Regulation of extracellular matrix Accession CO1A1_MOUSE CO1A2_MOUSE CO3A1_MOUSE CO5A2_MOUSE FINC_MOUSE FBLN2_MOUSE SPRC_MOUSE PEDF_MOUSE CO5A1_MOUSE HPT_MOUSE CATB_MOUSE IC1_MOUSE PAI1_MOUSE CO2A1_MOUSE TIMP1_MOUSE CO4A1_MOUSE TITIN_MOUSE PGS1_MOUSE CATL1_MOUSE EGFR_MOUSE COBA2_MOUSE FSTL1_MOUSE ATS7_MOUSE Description Collagen -1(I) chain Collagen -2(I) chain Collagen -1(III) chain Collagen -2(V) chain Fibronectin Fibulin-2 SPARC Pigment epithelium-derived factor Collagen -1(V) chain Haptoglobin Cathepsin B Plasma protease C1 inhibitor Plasminogen activator inhibitor 1 Collagen -1(II) chain Metalloproteinase inhibitor 1 Collagen -1(IV) chain Titin Biglycan Cathepsin L1 Epidermal growth factor receptor Collagen -2(XI) chain Follistatin-related protein 1 A disintegrin and metalloproteinase with thrombospondin motifs 7 Description Tenascin Adipocyte enhancer-binding protein 1 Collagen -2(I) chain Thrombospondin-1 Plasma protease C1 inhibitor EMILIN-1 Biglycan Collagen -1(I) chain Basement membrane-speciﬁc heparan sulfate proteoglycan core protein Decorin Collagen -1(III) chain Plasminogen activator inhibitor 1 Titin 14-3-3 protein / -2-macroglobulin Lipoprotein lipase -actin-like protein 2 Apolipoprotein E Inter- -trypsin inhibitor heavy chain H3 Histone H2B type 1-C/E/G Signal peptide? - - - - - Functional description Extracellular matrix Transcriptional repressor Extracellular matrix Angiogenesis inhibitor Inhibits C1s and C1r protease activity Extracellular matrix Extracellular matrix Extracellular matrix Accession TENA_MOUSE AEBP1_MOUSE COL1A2_MOUSE TSP1_MOUSE IC1_MOUSE EMIL1_MOUSE PGS1_MOUSE CO1A1_MOUSE Extracellular matrix PGBM_MOUSE Extracellular matrix PGS2_MOUSE Extracellular matrix CO3A1_MOUSE Serine protease inhibitor, inhibits matrix metalloproteinases, PAI1_MOUSE associated with insulin resistance Mechanical contraction TITIN_MOUSE Phosphoprotein binding, modulation of enzymatic activity 1433Z_MOUSE Serine protease inhibitor, prevents coagulation and ﬁbrinolysis A2M_MOUSE Lipoprotein associated lipid hydrolysis LIPL_MOUSE Cytoskeleton ACTBL_MOUSE Lipid transport APOE_MOUSE Serine protease inhibitor DNA binding ITIH3_MOUSE H2B1C_MOUSE Visceral or subcutaneous WAT-derived endothelial cells were incubated in media containing different isotopes of arginine and lysine. Conditioned media were mixed, subjected to lectin afﬁnity chromatography, and subjected to quantitative mass spectrometry. Peak height ratios of isotope to endogenous (12C) amino acids and ratios between isotopes are shown. Complete dataset available in supplementary data. diabetes.diabetesjournals.org DIABETES, VOL. 59, DECEMBER 2010 3013