Iranian J Parasitol: Vol. 6, No.2, 2011, pp.9-16 Fig.2: Caspase-3 activity in fertile HF-treated lymphocytes, infertile HF-treated lymphocytes and cell con- trol (from left to right). Bar graph indicates the mean ± S.E.M. Increase in Caspase-3 activity was deter- mined by comparing fluorescence of 7-amino-4-trifluoromethyl coumarin in control with HF-treated lymphocytes Fig.3: Semi-quantitative RT-PCR analysis of Bax and Bcl-2 expressions in fertile HF-treated lymphocytes, infertile HF-treated lymphocytes and cell control (from left to right). GAPDH (Gluceraldehydes-3-phos- phate dehydrogenase GAPDH gene) was used as an internal control. The density of the labeled bands for amplified products of Bax gene (151 bp) and Bcl-2 gene (249 bp) as well as GAPDH gene (500 bp) is shown in each group Fig. 4: Bax/Bcl-2 expression ratio in fertile HF-treated lymphocytes compared to infertile HF-treated lymphocytes and cell control. Bar graph indicates the mean ± S.E.M 13