Niepelt et al. Nanoscale Research Letters 2011, 6:511 http://www.nanoscalereslett.com/content/6/1/511 Page 5 of 7 Figure 3 Nanowire intensity fluctuations and thickness variations. In (a), a fluorescence image of a biofunctionalized nanowire is shown. In (b), the intensity plot along the dotted line is shown. The intensity value is fluctuating along the wire axis and even disappearing at two points. Images (c) and (d) show SEM images of a typical nanowire used in this study. With the larger magnification in (d), the nanowire thickness variation is clearly visible. appears to be orders of magnitude weaker in the latter case. In Figure 5c, the fluorescence intensities of various control samples are compared. To quantify the intensi- ties of different fluorescent samples, all signals have been integrated for different lengths of time and the resulting apparent magnitudes have been classified into six levels of fluorescence intensity between no fluores- cence at all and fluorescence saturation. It turns out that the fluorescence is most pronounced in the case of GOPS treatment and DNA with amino linker. All the