W158 Nucleic Acids Research, 2007, Vol. 35, Web Server issue GeneHub-GEPIS reliable, powerful, easy to use and widely accessible. At this point, GeneHub-GEPIS can report estimated expression levels in about 40 diﬀerent types of normal and cancerous tissues for a given gene or a list of genes. As more EST data become available, it will be possible to analyze gene expression in more detailed tissue subtypes and for additional organisms. We have noticed a dramatic increase of traﬃc to our web server since the release of GeneHub-GEPIS, and we hope that GeneHub-GEPIS will stimulate greater usage of EST data and perhaps additional software development in this area. SUPPLEMENTARY DATA Supplementary Data are available at NAR Online. ACKNOWLEDGEMENTS We thank the UCSF Computer Graphics Laboratory and Dr Thomas Ferrin for hosting this web server. We thank Fan Hsu and Jim Kent from UCSC for early evaluation and comments. 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