is revealed from the experimental data, SFmap could be implemented to enhance high accurate genome-wide pre- dictions of binding sites of SFs in particular and RNA-binding proteins in general. ACKNOWLEDGEMENT We would like to thank the many users for their useful comments and suggestions for improving the web site. FUNDING Elyahu Pen Research Fund; the Israeli Science Foundation (grant number 1297/09 to Y.M.G.). Funding for open access charge: The Israeli Science Foundation (grant number 1297/09 to Y.M.G.). Conﬂict of interest statement. None declared. REFERENCES 1. Chen,M. and Manley,J.L. (2009) Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Nat. Rev. Mol. Cell. Biol., 10, 741–754. 2. Cooper,T.A., Wan,L. and Dreyfuss,G. (2009) RNA and disease. Cell, 136, 777–793. 3. Schwartz,S., Hall,E. and Ast,G. (2009) SROOGLE: webserver for integrative, user-friendly visualization of splicing signals. Nucleic Acids Res., 37, W189–W192. 4. Wang,Z. and Burge,C.B. (2008) Splicing regulation: from a parts list of regulatory elements to an integrated splicing code. RNA, 14, 802–813. 5. Ladd,A.N. and Cooper,T.A. (2002) Finding signals that regulate alternative splicing in the post-genomic era. Genome Biol., 3, reviews0008. 6. Cartegni,L., Wang,J., Zhu,Z., Zhang,M.Q. and Krainer,A.R. (2003) ESEﬁnder: a web resource to identify exonic splicing enhancers. Nucleic Acids Res., 31, 3568–3571. 7. Fairbrother,W.G., Yeo,G.W., Yeh,R., Goldstein,P., Mawson,M., Sharp,P.A. and Burge,C.B. (2004) RESCUE-ESE identiﬁes Nucleic Acids Research, 2010, Vol. 38, Web Server issue W285 candidate exonic splicing enhancers in vertebrate exons. Nucleic Acids Res., 32, W187–W190. 8. Zhang,X.H., Kangsamaksin,T., Chao,M.S., Banerjee,J.K. and Chasin,L.A. (2005) Exon inclusion is dependent on predictable exonic splicing enhancers. Mol. Cell Biol., 25, 7323–7332. 9. Wang,Z., Rolish,M.E., Yeo,G., Tung,V., Mawson,M. and Burge,C.B. (2004) Systematic identiﬁcation and analysis of exonic splicing silencers. Cell, 119, 831–845. 10. Goren,A., Ram,O., Amit,M., Keren,H., Lev-Maor,G., Vig,I., Pupko,T. and Ast,G. (2006) Comparative analysis identiﬁes exonic splicing regulatory sequences–the complex deﬁnition of enhancers and silencers. Mol. Cell, 22, 769–781. 11. Chasin,L.A. (2007) Searching for splicing motifs. Adv. Exp. Med. Biol., 623, 85–106. 12. Smith,P.J., Zhang,C., Wang,J., Chew,S.L., Zhang,M.Q. and Krainer,A.R. (2006) An increased speciﬁcity score matrix for the prediction of SF2/ASF-speciﬁc exonic splicing enhancers. Hum. Mol. Genet., 15, 2490–2508. 13. Huang,H.Y., Chien,C.H., Jen,K.H. and Huang,H.D. (2006) RegRNA: an integrated web server for identifying regulatory RNA motifs and elements. Nucleic Acids Res., 34, W429–W434. 14. Akerman,M., David-Eden,H., Pinter,R.Y. and Mandel- Gutfreund,Y. (2009) A computational approach for genome-wide mapping of splicing factor binding sites. Genome Biol., 10, R30. 15. Kuhn,R.M., Karolchik,D., Zweig,A.S., Wang,T., Smith,K.E., Rosenbloom,K.R., Rhead,B., Raney,B.J., Pohl,A., Pheasant,M. et al. (2009) The UCSC Genome Browser Database: update 2009. Nucleic Acids Res., 37, D755–D761. 16. Kent,W.J. (2002) BLAT–the BLAST-like alignment tool. Genome Res., 12, 656–664. 17. Sureau,A., Gattoni,R., Dooghe,Y., Stevenin,J. and Soret,J. (2001) SC35 autoregulates its expression by promoting splicing events that destabilize its mRNAs. EMBO J., 20, 1785–1796. 18. Dreumont,N., Hardy,S., Behm-Ansmant,I., Kister,L., Branlant,C., Stevenin,J. and Bourgeois,C.F. (2009) Antagonistic factors control the unproductive splicing of SC35 terminal intron. Nucleic Acids Res., 38, 1353–1366. 19. Buratti,E. and Baralle,F.E. (2008) Multiple roles of TDP-43 in gene expression, splicing regulation, and human disease. Front. Biosci., 13, 867–878. 20. Gupta,Y.K., Lee,T.H., Edwards,T.A., Escalante,C.R., Kadyrova,L.Y., Wharton,R.P. and Aggarwal,A.K. (2009) Co-occupancy of two Pumilio molecules on a single hunchback NRE. RNA, 15, 1029–1035.