Online supplemental material. Fig. S1 shows CD4+ and CD8+ numbers in thymus, spleen, and LN of WT and SOCS2/ mice, increased IL-4, IL-5, IL-13, and GATA-3 mRNA expression in SOCS2-deficient CD4+ T cells by real-time PCR, and dot plot showing enhanced Th2 differen- tiation in the absence of SOCS2. Fig. S2 shows lung histology, cell in- filtration in BAL, and IL-4, IFN-, and IL-17 concentration in BAL of RAG-1/ mice reconstituted with PBS, WT, or SOCS2/ CD4+ T cells after OVA challenge. Fig. S3 shows increased STAT6 and STAT5 phos- phorylation and reduced STAT3 phosphorylation in SOCS2-deficient CD4+ T cells by flow cytometry, unaffected IL-4R levels, SOCS2 in- duction in CD4+ T cells after IL-6 and TGF- stimulation, and reduced RORt and IL-17 levels in SOCS2 deficient CD4+ T cells polarized toward Th17 lineage. Fig. S4 shows that in the absence of SOCS2, SOCS3 and SOCS1 levels are elevated in splenocytes, SOCS3 expression is higher in freshly isolated and Th2-polarized CD4+ T cells, and SOCS1 and SOCS3 mRNA expression is higher in CD4+ cells stimulated with anti-CD3 and anti-CD28. Online supplemental material is available at http://www.jem .org/cgi/content/full/jem.20101167/DC1. We would like to thank D. Hilton for providing the SOCS2/ mice (Royal Melbourne Hospital, Victoria 3050, Australia), S. Dubrac (Innsbruck Medical University, Austria) and L. Hanna (BRU, Queen’s University Belfast) for helping to design AD studies, C. Fulton (Queen’s University Belfast) for assistance with cell sorting, M. Walsh (City Hospital, Belfast) for helping with eosinophil counting, and O. Lyubomska and M. McCaigue, (Queen’s University Belfast) for assistance. P.G. Fallon was supported by Science Foundation Ireland (07/IN1/B902) and National Children’s Research Centre. J.A. Johnston, A. Kissenpfennig, and J. 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