Figure 2. Autoantibody production and renal pathology in DKO female mice. (A) Serum anti-dsDNA antibody levels of 1-yr-old female (top) and male (bottom) mice (n = 13–17/group). Each dot represents individual mice per group, and horizontal bars indicate mean for each group. Data are representative of two independent experiments. ***, P < 0.005; ns, not statistically significant. (B) Sera from 4 WT and 4 DKO 1-yr-old female mice were subjected to an auto- antigen microarray. The data are presented as a heat map, which shows the IgG seroreactivities of each sample toward the individual antigens on the array. Reactivities above the array mean are shown in red, reactivities below the mean are shown in green, and reactivities close to the mean are shown in black. A clustering algorithm was used to cluster together sera that display similar reactivities (top dendrogram) and group together antigens that were targeted in a 584 IRF4 in autoimmunity | Biswas et al.