for the phosphatidylinositol-phosphate kinase family. Crit. Rev. Biochem. Mol. Biol. 42:15–39. Helms, C., S. Pelsue, L. Cao, E. Lamb, B. Loffredo, P. Taillon-Miller, B. Herrin, L.M. Burzenski, B. Gott, B.L. Lyons, et al. 2005. The Tetratricopeptide repeat domain 7 gene is mutated in flaky skin mice: a model for psoriasis, autoimmunity, and anemia. Exp. Biol. Med. (Maywood). 230:659–667. Hendricks, K.B., B.Q. Wang, E.A. Schnieders, and J. Thorner. 1999. Yeast homologue of neuronal frequenin is a regulator of phosphatidylinositol- 4-OH kinase. Nat. Cell Biol. 1:234–241. Honda, A., M. Nogami, T. Yokozeki, M. Yamazaki, H. Nakamura, H. Watanabe, K. Kawamoto, K. Nakayama, A.J. Morris, M.A. Frohman, and Y. Kanaho. 1999. Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation. Cell. 99:521–532. Huang, F.D., H.J. Matthies, S.D. Speese, M.A. Smith, and K. Broadie. 2004. Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. Nat. Neurosci. 7:1070–1078. Huang, F.D., E. Woodruff, R. Mohrmann, and K. Broadie. 2006. Rolling blackout is required for synaptic vesicle exocytosis. J. Neurosci. 26:2369–2379. Hughes, T.R., M.J. Marton, A.R. Jones, C.J. Roberts, R. Stoughton, C.D. Armour, H.A. Bennett, E. Coffey, H. Dai, Y.D. He, et al. 2000. Functional discov- ery via a compendium of expression profiles. Cell. 102:109–126. Kaksonen, M., C.P. Toret, and D.G. Drubin. 2005. A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 123:305–320. Lee, S.Y., M.R. Wenk, Y. Kim, A.C. Nairn, and P. De Camilli. 2004. Regulation of synaptojanin 1 by cyclin-dependent kinase 5 at synapses. Proc. Natl. Acad. Sci. USA. 101:546–551. Lee, S.Y., S. Voronov, K. Letinic, A.C. Nairn, G. Di Paolo, and P. De Camilli. 2005. Regulation of the interaction between PIPKI and talin by proline- directed protein kinases. J. Cell Biol. 168:789–799. Lemmon, M.A. 2003. Phosphoinositide recognition domains. Traffic. 4:201–213. Lenburg, M.E., and E.K. O’Shea. 2001. Genetic evidence for a morphogenetic function of the Saccharomyces cerevisiae Pho85 cyclin-dependent kinase. Genetics. 157:39–51. Levine, T., and C. Loewen. 2006. Inter-organelle membrane contact sites: through a glass, darkly. Curr. Opin. Cell Biol. 18:371–378. Ling, K., R.L. Doughman, V.V. Iyer, A.J. Firestone, S.F. Bairstow, D.F. Mosher, M.D. Schaller, and R.A. Anderson. 2003. Tyrosine phosphorylation of type I phosphatidylinositol phosphate kinase by Src regulates an integ- rin–talin switch. J. Cell Biol. 163:1339–1349. Longtine, M.S., A. McKenzie III, D.J. Demarini, N.G. Shah, A. Wach, A. Brachat, P. Philippsen, and J.R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast. 14:953–961. Mnaimneh, S., A.P. Davierwala, J. Haynes, J. Moffat, W.T. Peng, W. Zhang, X. Yang, J. Pootoolal, G. Chua, A. Lopez, et al. 2004. Exploration of essen- tial gene functions via titratable promoter alleles. Cell. 118:31–44. Muhua, L., N.R. Adames, M.D. Murphy, C.R. Shields, and J.A. Cooper. 1998. A cytokinesis checkpoint requiring the yeast homologue of an APC-binding protein. Nature. 393:487–491. Odorizzi, G., M. Babst, and S.D. Emr. 2000. Phosphoinositide signaling and the regulation of membrane trafficking in yeast. Trends Biochem. Sci. 25:229–235. Osborne, S.L., P.J. Wen, and F.A. Meunier. 2006. Phosphoinositide regulation of neuroexocytosis: adding to the complexity. J. Neurochem. 98:336–342. Peng, W.T., M.D. Robinson, S. Mnaimneh, N.J. Krogan, G. Cagney, Q. Morris, A.P. Davierwala, J. Grigull, X. Yang, W. Zhang, et al. 2003. A panoramic view of yeast noncoding RNA processing. Cell. 113:919–933. Prodromou, C., G. Siligardi, R. O’Brien, D.N. Woolfson, L. Regan, B. Panaretou, J.E. Ladbury, P.W. Piper, and L.H. Pearl. 1999. Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)-domain co-chaperones. EMBO J. 18:754–762. Pruyne, D., L. Gao, E. Bi, and A. Bretscher. 2004. Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast. Mol. Biol. Cell. 15:4971–4989. Roy, A., and T.P. Levine. 2004. Multiple pools of phosphatidylinositol 4-phos- phate detected using the pleckstrin homology domain of Osh2p. J. Biol. Chem. 279:44683–44689. Rudge, S.A., D.M. Anderson, and S.D. Emr. 2004. Vacuole size control: regula- tion of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 com- plex, a PtdIns(3,5)P2-specific phosphatase. Mol. Biol. Cell. 15:24–36. Scheufler, C., A. Brinker, G. Bourenkov, S. Pegoraro, L. Moroder, H. Bartunik, F.U. Hartl, and I. Moarefi. 2000. Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multi- chaperone machine. Cell. 101:199–210. 1074 JCB • VOLUME 183 • NUMBER 6 • 2008 Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19–27. Simonsen, A., A.E. Wurmser, S.D. Emr, and H. Stenmark. 2001. The role of phos- phoinositides in membrane transport. Curr. Opin. Cell Biol. 13:485–492. Siniossoglou, S., E.C. Hurt, and H.R. Pelham. 2000. Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast. J. Biol. Chem. 275:19352–19360. Stefan, C.J., S.M. Padilla, A. Audhya, and S.D. Emr. 2005. The phosphoinosi- tide phosphatase Sjl2 is recruited to cortical actin patches in the control of vesicle formation and fission during endocytosis. Mol. Cell. Biol. 25:2910–2923. Strahl, T., H. Hama, D.B. DeWald, and J. Thorner. 2005. Yeast phosphatidyli- nositol 4-kinase, Pik1, has essential roles at the Golgi and in the nucleus. J. Cell Biol. 171:967–979. Tabuchi, M., A. Audhya, A.B. Parsons, C. Boone, and S.D. Emr. 2006. The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol. Cell. Biol. 26:5861–5875. Tarassov, K., V. Messier, C.R. Landry, S. Radinovic, M.M. Serna Molina, I. Shames, Y. Malitskaya, J. Vogel, H. Bussey, and S.W. Michnick. 2008. An in vivo map of the yeast protein interactome. Science. 320:1465–1470. Voelker, D.R. 2005. Protein and lipid motifs regulate phosphatidylserine traffic in yeast. Biochem. Soc. Trans. 33:1141–1145. Walch-Solimena, C., and P. Novick. 1999. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat. Cell Biol. 1:523–525. Walther, T.C., J.H. Brickner, P.S. Aguilar, S. Bernales, C. Pantoja, and P. Walter. 2006. Eisosomes mark static sites of endocytosis. Nature. 439:998–1003. Winzeler, E.A., D.D. Shoemaker, A. Astromoff, H. Liang, K. Anderson, B. Andre, R. Bangham, R. Benito, J.D. Boeke, H. Bussey, et al. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 285:901–906. Yoshida, S., Y. Ohya, M. Goebl, A. Nakano, and Y. Anraku. 1994. A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae. J. Biol. Chem. 269:1166–1172. Yu, J.W., J.M. Mendrola, A. Audhya, S. Singh, D. Keleti, D.B. DeWald, D. Murray, S.D. Emr, and M.A. Lemmon. 2004. Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Mol. Cell. 13:677–688. Zhang, X., K. Orlando, B. He, F. Xi, J. Zhang, A. Zajac, and W. Guo. 2008. Membrane association and functional regulation of Sec3 by phospholipids and Cdc42. J. Cell Biol. 180:145–158.