Table II. Phenotypic Characterization of Multicolony-derived Strains of Human Marrow Stromal Cells and Guinea Pig Blood-derived Adherent Cells, and of Single Colony–derived Strains of Human and Rabbit Blood-derived Adherent Cells Marker Fibroblastic Col I (LF41)* Col III (LF71)* Fibronectin (rabbit; ICN Biomedicals) Fibronectin (guinea pig, MsGP13; Serotec) CD44 (H9H11)‡ 1 integrin subunit (MAB1959, Chemicon) Osteogenic ON (BON-1)* OPN (LF123)* BSP (LF83)* OC (LF32)* Adipocytic CEBP (Santa Cruz Biotechnology, Inc.) PPAR 2 (Santa Cruz Biotechnology, Inc.) Endothelial CD34 (Dako) Muc-18§ Factor VIII (Dako) Endoglin PAL-E (rabbit; Accurat Chemical Scientific) EN4 (guinea pig; Accurat Chemical Scientific) Marrow stromal cells Stro-1¶ CD106 (VCAM-1) (human, MCA907; Serotec) Smooth muscle -Smooth muscle actin (1A4; Dako) Smooth muscle (rabbit and guinea pig, ACL-10002; Accurat Chemical Scientific) Skeletal muscle MyoD (M318; Dako) Monocyte/macrophage CD14 (human, TUK4; Dako) RAM11 (rabbit; Dako) MR-1 (guinea pig; Serotec) Leukocyte CD45 (human, 2B11/PD7-26; Dako) CD45 (rabbit, L12/201; Serotec) CD45 (guinea pig, 1H-1; Serotec) Nerve Neurofilament (2F11; Dako) Histochemical Alkaline phosphatase Acid phosphatase -Naphthyl acetate esterase Oil Red O (lipid accumulation) 20% FBS 20% Rabbit serum Rabbit polygonal non-osteogenic Rabbit fibroblast-like osteogenic Guinea pig osteogenic Human fibroblast-like non-osteogenic Human fibroblast-like osteogenic Human marrow stromal cells / / / , intensive staining; , weak staining; , negative staining. *Fisher et al. (1995); provided by Dr. L. Fischer, National Institutes of Dental and Craniofacial Research, National Institutes of Health (Bethesda, MD). ‡Zannettino et al. (1996). §Filshie et al. (1998). Developmental studies hybridoma Bank, University of Iowa, Iowa City, IA. ¶Simmons and Torok-Storb (1991). lagen types I and III, fibronectin, osteonectin, -smooth muscle actin, CD44, VCAM-1, and the 1 integrin subunit. The phenotypic profile of blood-borne adherent cells over- all was nearly identical between species (rabbit, guinea pig, and human) and quite similar to that of marrow stromal cells derived from each species. However, the human blood-derived adherent cells were negative for the human marrow stromal marker, Stro-1, as well as for endoglin and The Journal of Cell Biology, Volume 153, 2001 1136