672 The Journal of Cell Biology | Volume 166, Number 5, 2004 W.K. Yung, and P.A. Steck. 1999. Regulation of Akt/PKB activity, cellular growth, and apoptosis in prostate carcinoma cells by MMAC/PTEN. Cancer Res. 59:2551–2556. Dolmetsch, R.E., K. Xu, and R.S. Lewis. 1998. Calcium oscillations increase the efficiency and specificity of gene expression. Nature. 392:933–936. Ellis-Davies, G.C., and J.H. Kaplan. 1994. Nitrophenyl-EGTA, a photolabile che- lator that selectively binds Ca2 with high affinity and releases it rapidly upon photolysis. Proc. Natl. Acad. Sci. USA. 91:187–191. Gilmore, T.D. 1999. Multiple mutations contribute to the oncogenicity of the ret- roviral oncoprotein v-Rel. Oncogene. 18:6925–6937. Guttridge, D.C., C. Albanese, J.Y. Reuther, R.G. Pestell, and A.S. Baldwin Jr. 1999. NF- B controls cell growth and differentiation through transcrip- tional regulation of cyclin D1. Mol. Cell. Biol. 19:5785–5799. Hinz, M., D. Krappmann, A. Eichten, A. Heder, C. Scheidereit, and M. Strauss. 1999. NF- B function in growth control: regulation of cyclin D1 expression and G0/G1-to-S-phase transition. Mol. Cell. Biol. 19:2690–2698. Ichikawa, J., and T. Kiyohara. 2001. Suppression of EGF-induced cell prolifera- tion by the blockade of Ca2 mobilization and capacitative Ca2 entry in mouse mammary epithelial cells. Cell Biochem. Funct. 19:213–219. Joyce, D., B. Bouzahzah, M. Fu, C. Albanese, M. D’Amico, J. Steer, J.U. Klein, R.J. Lee, J.E. Segall, J.K. Westwick, et al. 1999. Integration of Rac-depen- dent regulation of cyclin D1 transcription through a nuclear factor- B- dependent pathway. J. Biol. Chem. 274:25245–25249. Katoch, S.S., X. Su, and R.S. Moreland. 1999. Ca2 - and protein kinase C-depen- dent stimulation of mitogen-activated protein kinase in detergent-skinned vascular smooth muscle. J. Cell. Physiol. 179:208–217. Lannergren, J., and A. Arner. 1992. Relaxation rate of intact striated muscle fibres after flash photolysis of a caged calcium chelator (diazo-2). J. Muscle Res. Cell Motil. 13:630–634. Lau, L.F., and D. Nathans. 1985. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells. EMBO J. 4:3145–3151. Lavoie, J.N., G. L’Allemain, A. Brunet, R. Muller, and J. Pouyssegur. 1996. Cyclin D1 expression is regulated positively by the p42/p44MAPK and negatively by the p38/HOGMAPK pathway. J. Biol. Chem. 271:20608–20616. Lee, R.J., C. Albanese, R.J. Stenger, G. Watanabe, G. Inghirami, G.K. Haines III, M. Webster, W.J. Muller, J.S. Brugge, R.J. Davis, and R.G. Pestell. 1999. pp60v-src induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcrip- tion factor-2 in pp60v-src signaling in breast cancer cells. J. Biol. Chem. 274: 7341–7350. Lenormand, P., C. Sardet, G. Pages, G. L’Allemain, A. Brunet, and J. Pouyssegur. 1993. Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fi- broblasts. J. Cell Biol. 122:1079–1088. Lilienbaum, A., and A. Israel. 2003. From calcium to NF- B signaling pathways in neurons. Mol. Cell. Biol. 23:2680–2698. Madrid, L.V., M.W. Mayo, J.Y. Reuther, and A.S. Baldwin Jr. 2001. Akt stimu- lates the transactivation potential of the RelA/p65 Subunit of NF- B through utilization of the I B kinase and activation of the mitogen-activated protein kinase p38. J. Biol. Chem. 276:18934–18940. Matsumura, I., T. Kitamura, H. Wakao, H. Tanaka, K. Hashimoto, C. Albanese, J. Downward, R.G. Pestell, and Y. Kanakura. 1999. Transcriptional regula- tion of the cyclin D1 promoter by STAT5: its involvement in cytokine- dependent growth of hematopoietic cells. EMBO J. 18:1367–1377. McNeil, P.L., M.P. McKenna, and D.L. Taylor. 1985. A transient rise in cytosolic calcium follows stimulation of quiescent cells with growth factors and is in- hibitable with phorbol myristate acetate. J. Cell Biol. 101:372–379. Means, A.R. 1994. Calcium, calmodulin and cell cycle regulation. FEBS Lett. 347:1–4. Meng, F., L. Liu, P.C. Chin, and S.R. D’Mello. 2002. Akt is a downstream target of NF- B. J. Biol. Chem. 277:29674–29680. Nelson, G., L. Paraoan, D.G. Spiller, G.J. Wilde, M.A. Browne, P.K. Djali, J.F. Unitt, E. Sullivan, E. Floettmann, and M.R. White. 2002a. Multi-parame- ter analysis of the kinetics of NF- B signalling and transcription in single living cells. J. Cell Sci. 115:1137–1148. Nelson, G., G.J. Wilde, D.G. Spiller, E. Sullivan, J.F. Unitt, and M.R. White. 2002b. Dynamic analysis of STAT6 signalling in living cells. FEBS Lett. 532:188–192. Ozes, O.N., L.D. Mayo, J.A. Gustin, S.R. Pfeffer, L.M. Pfeffer, and D.B. Donner. 1999. NF- B activation by tumour necrosis factor requires the Akt serine- threonine kinase. Nature. 401:82–85. Pages, G., P. Lenormand, G. L’Allemain, J.C. Chambard, S. Meloche, and J. Pouyssegur. 1993. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation. Proc. Natl. Acad. Sci. USA. 90: 8319–8323. Patel, R., M. Holt, R. Philipova, S. Moss, H. Schulman, H. Hidaka, and M. Whit- aker. 1999. Calcium/calmodulin-dependent phosphorylation and activation of human Cdc25-C at the G2/M phase transition in HeLa cells. J. Biol. Chem. 274:7958–7968. Rayet, B., and C. Gelinas. 1999. Aberrant rel/nfkb genes and activity in human cancer. Oncogene. 18:6938–6947. Rocha, S., A.M. Martin, D.W. Meek, and N.D. Perkins. 2003. p53 represses cy- clin D1 transcription through down regulation of Bcl-3 and inducing in- creased association of the p52 NF- B subunit with histone deacetylase 1. Mol. Cell. Biol. 23:4713–4727. Romashkova, J.A., and S.S. Makarov. 1999. NF- B is a target of AKT in anti-apop- totic PDGF signalling. Nature. 401:86–90. Romieu-Mourez, R., D.W. Kim, S.M. Shin, E.G. Demicco, E. Landesman-Bollag, D.C. Seldin, R.D. Cardiff, and G.E. Sonenshein. 2003. Mouse mammary tumor virus c-rel transgenic mice develop mammary tumors. Mol. Cell. Biol. 23:5738–5754. Rozen, S., and H.J. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132:365–386. Ryan, K.M., M.K. Ernst, N.R. Rice, and K.H. Vousden. 2000. Role of NF- B in p53-mediated programmed cell death. Nature. 404:892–897. Santella, L. 1998. The role of calcium in the cell cycle: facts and hypotheses. Bio- chem. Biophys. Res. Commun. 244:317–324. See, V., A.L. Boutillier, H. Bito, and J.P. Loeffler. 2001. Calcium/calmodulin- dependent protein kinase type IV (CaMKIV) inhibits apoptosis induced by potassium deprivation in cerebellar granule neurons. FASEB J. 15:134–144. Sen, R., and D. Baltimore. 1986. Inducibility of kappa immunoglobulin enhancer- binding protein Nf- B by a posttranslational mechanism. Cell. 47:921–928. Takuwa, N., W. Zhou, M. Kumada, and Y. Takuwa. 1995. Involvement of intact inositol-1,4,5-trisphosphate-sensitive Ca2 stores in cell cycle progression at the G1/S boundary in serum-stimulated human fibroblasts. FEBS Lett. 360: 173–176. Tang, E.D., N. Inohara, C.Y. Wang, G. Nunez, and K.L. Guan. 2003. Roles for homotypic interactions and transautophosphorylation in I B kinase (IKK ) activation. J. Biol. Chem. 278:38566–38570. Thanos, D., and T. Maniatis. 1995. NF- B: a lesson in family values. Cell. 80: 529–532. Truneh, A., F. Albert, P. Golstein, and A.M. Schmitt-Verhulst. 1985. Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester. Nature. 313:318–320. Watanabe, G., C. Albanese, R.J. Lee, A. Reutens, G. Vairo, B. Henglein, and R.G. Pestell. 1998. Inhibition of cyclin D1 kinase activity is associated with E2F- mediated inhibition of cyclin D1 promoter activity through E2F and Sp1. Mol. Cell. Biol. 18:3212–3222. Westerheide, S.D., M.W. Mayo, V. Anest, J.L. Hanson, and A.S. Baldwin Jr. 2001. The putative oncoprotein Bcl-3 induces cyclin D1 to stimulate G1 transition. Mol. Cell. Biol. 21:8428–8436. Whitaker, M., and M.G. Larman. 2001. Calcium and mitosis. Semin. Cell Dev. Biol. 12:53–58.