Figure 4. Min mice have mitotic defects in dividing crypt cells. (A) Crypts in the small intestine were analyzed using ﬂuorescence microscopy. The cellular boundaries were identiﬁed with β-catenin (red), spindles with Numa antibodies (green), and chromosomes with DAPI (blue); a twofold magniﬁcation of the boxed area is shown in the inset. (B) Diagram of the cellular organization, position of mitotic cells, and orientation of their spindles for a wild-type mouse. 1114 JCB • VOLUME 178 • NUMBER 7 • 2007