Syntichaki, P., C. Samara, and N. Tavernarakis. 2005. The vacuolar H+-ATPase mediates intracellular acidification required for neurodegeneration in C. elegans. Curr. Biol. 15:1249–1254. Takemura, H., A.R. Hughes, O. Thastrup, and J.W. Putney Jr. 1989. Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phos- phate regulates calcium fluxes at the plasma membrane. J. Biol. Chem. 264:12266–12271. Treusch, S., S. Knuth, S.A. Slaugenhaupt, E. Goldin, B.D. Grant, and H. Fares. 2004. Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 is required for lysosome biogenesis. Proc. Natl. Acad. Sci. USA. 101:4483–4488. Walker, N.I., B.V. Harmon, G.C. Gobe, and J.F. Kerr. 1988. Patterns of cell death. Methods Achiev. Exp. Pathol. 13:18–54. Xu, K., N. Tavernarakis, and M. Driscoll. 2001. Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum. Neuron. 31:957–971. Yamashima, T. 2000. Implication of cysteine proteases calpain, cathepsin and caspase in ischemic neuronal death of primates. Prog. Neurobiol. 62:273–295. Yamashima, T. 2004. Ca2+-dependent proteases in ischemic neuronal death: a conserved ‘calpain-cathepsin cascade’ from nematodes to primates. Cell Calcium. 36:285–293. Yamashima, T., Y. Kohda, K. Tsuchiya, T. Ueno, J. Yamashita, T. Yoshioka, and E. Kominami. 1998. Inhibition of ischaemic hippocampal neuronal death in primates with cathepsin B inhibitor CA-074: a novel strategy for neu- roprotection based on ‘calpain-cathepsin hypothesis’. Eur. J. Neurosci. 10:1723–1733. Yamashima, T., A.B. Tonchev, T. Tsukada, T.C. Saido, S. Imajoh-Ohmi, T. Momoi, and E. Kominami. 2003. Sustained calpain activation associated with lysosomal rupture executes necrosis of the postischemic CA1 neu- rons in primates. Hippocampus. 13:791–800. Yoshida, M., T. Yamashima, L. Zhao, K. Tsuchiya, Y. Kohda, A.B. Tonchev, M. Matsuda, and E. Kominami. 2002. Primate neurons show different vulnerability to transient ischemia and response to cathepsin inhibition. Acta Neuropathol. (Berl.). 104:267–272. Yu, G., R. Zucchi, S. Ronca-Testoni, and G. Ronca. 2000. Protection of ischemic rat heart by dantrolene, an antagonist of the sarcoplasmic reticulum cal- cium release channel. Basic Res. Cardiol. 95:137–143. Zhao, H.F., X. Wang, and G.J. Zhang. 2005. Lysosome destabilization by cyto- solic extracts, putative involvement of Ca(2+)/phospholipase C. FEBS Lett. 579:1551–1556. LYSOSOMAL FATE IN NECROTIC CELL DEATH • ARTAL-SANZ ET AL. 239