TRIF Can Be a Suppressor of TLR Signaling Figure 5. TRIF negatively regulates rEA-mediated MyD88 dependent activation of pro-inflammatory cytokines and chemokines in dendritic cells. (A) CD11c+ dendritic cells were isolated from C57BL/6 WT (N = 2), MyD88-KO (N = 2), TRIF-KO (N = 2), and MyD88/TRIF-DKO (N = 2) mice, in vitro stimulated with rEA, then used to perform a IL12p70 ELISA as described in Materials and Methods. One (of three) representative PLoS ONE | www.plosone.org 9 July 2011 | Volume 6 | Issue 7 | e22064