Characterization of TgICMAP1 Figure 2. eGFP-TgICMAP1 coats MTs when ectopically expressed in mammalian cells. Images of mouse embryonic fibroblast cells transiently expressing eGFP-TgICMAP1 (green) labeled with anti-tubulin YL1/2 (red), which show that eGFP-TgICMAP1 binds to a subset of MTs, mainly around the nucleus. Insets: notice that MT segments labeled by eGFP-TgICMAP1 tend to have much weaker staining of tubulin antibody than adjacent segments (arrowheads and brackets). Images are projections of deconvolved 3-D stacks. Insets are single optical sections of indicated regions in images and are at 26 magnification. doi:10.1371/journal.pone.0007406.g002 Figure 3. TgICMAP1 is a coiled-coil protein and contains a SMC-like coiled-coil domain. (A) Graphic display of predicted coiled-coil domains in TgICMAP1. Y-axis, probability of formation of coiled-coil domain where values close to 1 indicate regions with a high propensity to form coiled-coil domains. X-axis, TgICMAP1 amino acids. (B) Schematics of full-length TgICMAP1 and the two TgICMAP1 truncation proteins, TgICMAP11–474 and TgICMAP1455–1231. doi:10.1371/journal.pone.0007406.g003 PLoS ONE | www.plosone.org 3 October 2009 | Volume 4 | Issue 10 | e7406