Nuclease Processing of BIR Intermediates Figure 4. Genetic analysis of BIR intermediates processing in SSE mutants. (A) (B) Frequency of translocants of the different strains tested. See Material and Methods for details. ** and *, differences with the WT statistically significant (p,0.01 and p,0.05, respectively, x2 with Yates’ correction) u, statistically different from mus81D (p,0.01, x2 with Yates’ correction). Error bars represent standard deviations. (C) Appearance of BIR products as monitored by Southern analysis in HMR-inc WT, HMR-inc pol32D and HMR-inc mus81D yen1D strains. Experiments were performed as described in Figure 2C. A representative Southern analysis is shown for each genotype analyzed. Positions of the bands corresponding to 7/3-MAT, PLOS Genetics | www.plosgenetics.org 8 September 2012 | Volume 8 | Issue 9 | e1002979