Figure S5 HeLa cells were transfected with either empty pEGFP-C3 or pEGFP-C3-Aurora A constructs and cells were harvested 48 hrs after transfection for immunoprecipitations using anti-centrin and anti-phospho-centrin antibodies. Immunoprecip- itations were separated by SDS-PAGE and blotted with anti- centrin antibody. Note that there is more centrin and phospho- centrin in the pEGFP-C3-Aurora A expressing cells. (DOC) Figure S6 HeLa cells were processed for immunofluorescence as described in the Material and Methods section. Aurora A was detected with an anti-Aurora A antibody (green) and DNA was counterstained with DAPI (blue). An interphase cell is shown on the left side and an early prophase cell on the right side of the image. Note that Aurora A is much more abundant in the early prophase cell. Scale bar = 10 microns. (DOC) References 1. Duensing A, Liu Y, Perdreau SA, Kleylein-Sohn J, Nigg EA, et al. 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