SSC Niche and SSC In Zebrafish Figure 4. Depletion of endogenous spermatogenesis in male recipients for SSC transplantation. A. Effects of different temperatures on zebrafish GSI (gonadosomatic index). Bars represent mean 6 SE (n = 15, 20uC), (n = 12, 27uC), (n = 7, 30uC), and (n = 12, 35uC). Different letters indicate significant differences (p,0.05) among groups. B. Effects of two single doses of busulfan (30 or 40 mg/Kg) on zebrafish GSI. The arrow indicates the PLoS ONE | www.plosone.org 8 September 2010 | Volume 5 | Issue 9 | e12808