Actin Patch mRNA Localization and Dynamics Figure 3. Quantification of actin patch nucleator mRNA localization. (A) Proportion of total mRNAs localizing to the actin patch from cells at all stages of the cell cycle. Error bars represent the 95% confidence interval. (B) Comparison of mRNA localization patterns for pairs of MS2-tagged mRNAs. Cellular structures scored for mRNA localization are indicated in the first ideogram. Significant differences in localization between mRNAs of interest to cellular locations in the ideograms are indicated by color: red or blue indicates a –log false discovery rate (FDR) of 2 (light color) to 16 (saturated, dark color), with red colors indicating significant over-representation of mRNA 1 in each pair and blue colors indicating significant over- representation of mRNA 2. Grey or white represents no significant difference in the localization between the two mRNAs. Significance was measured by Fisher’s exact test with the Benjamini-Hochberg multiple hypothesis test correction. doi:10.1371/journal.pone.0031912.g003 proportion of BBC1 mRNA co-localizing at actin patches (P = 1.661027), while addition of the drug carrier, DMSO, had no significant effect (P = 0.46). To test the importance of Myo3 in AP mRNA localization to actin patches, we constructed homozygous MYO3 (myo3D) diploid versions of the BBC1:MS2 SAC6:mCherry, LAS17:MS2 SAC6:mCherry, and ARG3:MS2 SAC6:mCherry strains (see Materials and Methods). Loss of MYO3 significantly decreased the proportion of BBC1 and LAS17 mRNAs that localized to the actin patch (22% and 14%, respectively) while having no significant effect on the localization of ARG3 (5%; P = 5.661025, P = 1.161025, and P = 0.50, respectively). MYO3 deletion does not impact the formation of actin patches [76], nor did the strains exhibit any obvious defects in growth rate, PLoS ONE | www.plosone.org 6 February 2012 | Volume 7 | Issue 2 | e31912