MoAbs to Toxic Gluten Peptides Figure 5. Relative affinity of moAb A1 for different peptide variants derived from its recognition region (QLPYPQP). A. Amino acid sequences of the peptides. The A1 recognition sequence in the 33-mer peptide is in red. B, C, D and E. Competition assay for detection of the affinity of the moAb A1 for the peptides and their localization in a-gliadin (B; accession number: JQ1047), c-secalin (C; accession number: ABO32294.1), C- hordein (D; accession number: AAA92333.1) and avenin (E; accession number: AAA32716.1). Two separate assays were performed with the moAb, each with three repetitions. IC50 values of the moAb A1 to peptides are indicated. N.A.: Not applicable. The color code for labelling the peptides is the same as that used in A. doi:10.1371/journal.pone.0002294.g005 PLoS ONE | www.plosone.org 7 May 2008 | Volume 3 | Issue 5 | e2294