BLM and PICH in Centromere Disjunction Figure 1. GFP-BLM localizes to centromeres in G2/prophase cells. (A) Localization of GFP-BLM (green) to centromeres in late G2 cells. Nuclei were visualized by DAPI staining (blue). G2 cells were stained with antibodies against cyclin B1. Cells were stained with CREST serum to visualize centromeres (red). Lower panels are the magnification of the corresponding upper panels. Scale bar = 5 mm. (B) Two hundred cells with GFP-BLM- positive centromeres were analyzed by staining for cyclin B1 (cyclin B- or cyclin B+). The percentage of cells in each category is indicated (left panel), PLoS ONE | www.plosone.org 2 April 2012 | Volume 7 | Issue 4 | e33905