Locostatin Impairs Migration Figure 7. Locostatin disrupts the mitotic spindle and chromosome organization. MEFs expressing (A) wild-type (RKIP+/+) or lacking RKIP were plated at 26104 cells/coverslip and grown for 48 h before treatment with either DMSO (Control) or locostatin (20 mM) for either 1 or 2 h. (B) Wild-type (RKIP+/+) or deficient (RKIP2/2) RKIP were plated as described in (A) and treated with either DMSO (Control), locostatin (20 mM) or paclitaxel (10 mM) for 6 h. Cells were fixed and permeabilized and stained with anti-a-tubulin antibody-green. DNA/chromatin was stained with H o¨echst 33342- blue. Digital photomicrographs were captured using Openlab Darkroom (Improvision) and a Retiga 1300 color digital camera (Q Imaging). Intensity of images were adjusted to display qualitative rather than quantitative comparisons so that the intensity of staining in each panel cannot be directly compared. doi:10.1371/journal.pone.0006028.g007 PLoS ONE | www.plosone.org 10 June 2009 | Volume 4 | Issue 6 | e6028