Quantitative Mcm Perturbation in Mice Figure 1. MCM2-7 proteins and mRNA are reduced in Mcm4Chaos3/Chaos3 MEFs, particularly in early S phase. (A) Western blot analysis of MCM2-7 (left panel). Soluble or chromatin-bound protein was electrophoresced on PAGE gels, electrotransferred, and the blots were immunolabeled with the indicated antibodies. The bands correspond to the predicted molecular weights of these proteins, and for MCM2, the identity of the band was verifed by RNAi knockdown and transient overexpression in NIH 3T3 cells. TBP = TATA box binding protein. Quantification of Western blot data PLoS Genetics | www.plosgenetics.org 3 September 2010 | Volume 6 | Issue 9 | e1001110