SSC Niche and SSC In Zebrafish Figure 7. Characterization of male recipients prior SSC transplantation. A. 11-KT plasma levels (ng/ml plasma) from zebrafish kept at 27uC (control) (n = 7), 35uC (n = 11), and busulfan-depleted animals (n = 19). Bars represent the mean6SE. Different letters mean significant differences (p,0.05) among groups. B. In vitro 11-KT (ng/mg testis) release from control (27uC) (n = 7) and busulfan-depleted testes [35uC + Bu (40 mg/Kg/BW)] (n = 7) in basal and 1 mM forskolin-induced. Bars represent the mean6SE. * means significant differences (p,0.05) between control and depleted in the same experimental condition (Student unpaired t-test). # indicates significantly higher (p,0.05) than the respective basal release (Student paired PLoS ONE | www.plosone.org 13 September 2010 | Volume 5 | Issue 9 | e12808