Pathogen-Host Omics Data Figure 3. Structured queries across infectious agents and data types. A) Search for proteins detected in studies of Bacillus anthracis AND Salmonella typhimurium infection that include microarray experiments, finds 16 host proteins from mouse macrophage cell lines. B) Results include a large uncharacterized protein K1033_MOUSE. C) A customizable web interface allows users to add and view related information on pathways of protein families. To reproduce these queries use this URL: http://pir.georgetown.edu/cgi-bin/textsearch_cat.pl?search = 1&field0 = EXPC&query0 = Infection+Bacillus+anthracis&andor1 = and&field1 = EXPC&query1 = Infection+Salmonella+typhimurium&andor2 = and&field2 = datatype& query2 = microarray . doi:10.1371/journal.pone.0007162.g003 chemokine-binding proteins that mimic the chemokine response and prevent activation and chemotaxis of leukocytes [43,44]. Protein C6L belongs to a family of poxvirus paralogs that may function as toll-like receptor inhibitors based on homology to A52R [45,46,47]. Thus, this protein may modulate Toll/IL-1R signaling, resulting in a diminished host immune response and enhancing viral survival. P19013 - Keratin, type II cytoskeletal 4, the host protein, has tissue specificity in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa [48]. Transgenic knockout mice have shown Keratin, type II cytoskeletal 4 to play an important role in maintaining normal epithelial tissue structure [49]. Keratin Figure 4. Queries on cluster or family information. Queries on cluster or family information can discover related information across laboratories and host pathogen systems. Using the UniRef90 cluster ID for K1033_MOUSE, identified in Figure 3, to query for all proteins with at least 90% identity, we find the human homolog K1033_HUMAN was detected in HeLa cells infected with Vaccinia and Monkypox virus [12]. doi:10.1371/journal.pone.0007162.g004 PLoS ONE | www.plosone.org 7 September 2009 | Volume 4 | Issue 9 | e7162