Nuclear Translocation of Procaspase-3 by TRB3 Figure 4. TRB3 expression induces nuclear translocation of proCASP3. (A) HeLa cells were cotransfected with the C163S-proCASP3-EmGFP and V5-WT-TRB3 expression plasmids. After 24 hr, the cells were stained with anti-V5 antibody (red) and counterstained with DAPI (blue). Arrow and PLoS ONE | www.plosone.org 7 August 2012 | Volume 7 | Issue 8 | e42721