Table 8. Primers for Methylation Sensitive Restriction Fingerprinting (MSRF), Bisulfite Genomic Sequencing, Methylation Specific PCR (MSPCR) and Real-time PCR. Methylation Sensitive Restriction Fingerprinting Bs7 Bs10 Bs11 Bs20 Bs21 Bs22 Bs23 hBS_ACSL3_F1 hBS_ACSL3_R1 hBS_RAD21_F1 hBS_RAD21_R1 hBS_DUSP22_F1 hBS_DUSP22_R1 hBS_SCD5_F1 hBS_SCD5_R1 hBS_SFMBT2_F2 hBS_SFMBT2_R2 hBS_WWOX_F1 hBS_WWOX_R1 MS_ACSL3_MF-R1 MS_ACSL3_MR-R1 MS_ACSL3_UF-R1 MS_ACSL3_UR-R1 MS_ACSL3_MF-R2 MS_ACSL3_MR-R2 MS_ACSL3_UF-R2 MS_ACSL3_UR-R2 MS_ACSL3_MF-R3 MS_ACSL3_MR-R3 MS_ACSL3_UF-R3 MS_ACSL3_UR-R3 Real-time PCR hACSL3_F1 hACSL3_R1 hRAD21_F1 hRAD21_R1 hDUSP22_F1 hDUSP22_R1 hSCD5_F1 hSCD5_R1 hSFMBT2_F1 hSFMBT2_R1 hWWOX_F1 hWWOX_R1 59- GAGGTGCGCG-39 59- AGGGGACGCG-39 59- GAGAGGCGCG-39 59- GCGCCGACGT-39 59- CGGGACGCGA-39 59- CCGCGATCGC-39 59- TGGCCGCCGA-39 59-GGATTTGATAGTAATTTTTGTAAGAA-39 59-ACCTAATAACACCTACCCCACAAA-39 59-TTTAAAATAAGTATAAGAATAAAGTTAAA-39 59-AAAAAACAACAACAATCACTAACC-39 59-GTTAAAGGGGGATTTGGAGATT-39 59-TCACAAACACACACACAAAATCTAA-39 59-GGGATTGGAAAAATATTATAAAAGGTATT-39 59-ACAAATTCCCACTAACCACATAAAA-39 59-TTAAGATTGTTTTGGGGATTGAAT-39 59-TCCTAAACTAAAAAAAACCCCTAAC-39 59-TGTAAAATAAAGGTTGTGAATTAGTA-39 59-ACCCCTACACTCCAACCTAAATAAC-39 59-TTTTTGGTCGATTTCGTTTTC-39 59-AAACTACCACCCATTAACCCG-39 59-GTTTTTTTTGGTTGATTTTGTTTTT-39 59AAACTACCACCCATTAACCCAC–39 59-TCGTTTTTTTTGGTCGATTTC-39 59-ATACGAAAAAACGAACGTATACGAC-39 5-GTTTGTTTTTTTTGGTTGATTTTGT-39 59-CAATACAAAAAAACAAACATATACAAC-39 59-TTTAGGCGGTTTCGTTTAATAGAC-39 59-ATACGAAAAAACGAACGTATACGAC-39 59-TTAGGTGGTTTTGTTTAATAGATGT-39 59-ATACAAAAAAACAAACATATACAAC-39 59-GAGAGTTTGAACCCGATGGA-39 59-TTGGCACAACAAATCCAATG-39 59-AGAGCTTCCCCCAGAAGAAC-39 59-AAGAGCACGCTGAAGACCAT-39 59-AGGAGCGTGACACTGGTGAT-39 59-CTGCCGATACTGATGGACCT-39 59-GACCTGCTTGCTGATCCTGT-39 59-GGGCTGATGTGCTTGTCATA-39 59-GGGATTGGGTAATTTTATTTTTTTT-39 59-GACCTCCGTTTCTTCTGCAC-39 59-CGGATGGGAACAAGAAACTG-39 59-CAACCACTTTGCCAGTGAAA-39 Bisulfite Genomic Sequencing Methylation Specific PCR doi:10.1371/journal.pone.0004488.t008 PLoS ONE | www.plosone.org 13 February 2009 | Volume 4 | Issue 2 | e4488 PAH, Epigenetic and Asthma Logistic regression was performed to evaluate the association between parental report of asthma (dependent variable) and methylation status (independent variable). 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