Identification of DAF-12 Target Genes by ChIP-chip Figure 7. DAF-12 represses expression of target genes during dauer formation. (A) mRNA levels for heterochronic genes (white bars) or miRISC genes (grey bars) in L3 worms (daf-2; daf-12(rh61rh411)) or dauers (daf-2) grown at 25uC were quantified by NanoString analysis. The fold change for daf-2 dauer relative to L3 levels are shown (B) Images of lin-42p::GFP or lin-42p(mut)::GFP worms in L3 stage (left panels) or dauer stage (right panels). Expression of gfp or endogenous lin-42 in L3 and dauers stage worms as shown by RT-qPCR (graphs). (C) Detail of the pharynx from transgenic worms dauer arrested with increased exposure. Arrows indicate GFP expression. (D) Expression of endogenous lin-42 in L3 and dauer stage worms as shown by RT-qPCR. Bars denote standard error of mean (SEM). P-values were determined by Student’s t-test. * p,0.05, ** p,0.01, *** p,0.001. doi:10.1371/journal.pgen.1002179.g007 PLoS Genetics | www.plosgenetics.org 11 July 2011 | Volume 7 | Issue 7 | e1002179