TSE Transmission to Sea Bream Figure 7. Staining of an aggregate in the brain of a BSE-challenged fish 24 months p.i. with different techniques. A, H&E; B, Congo red in normal light; C, PAS; D, IHC (SaurPrP1) after PK-digestion; E, Alcian blue. Rectangles in the left panel indicate areas of magnification shown in the right panel. Scale bars, 100 mm (left panel) or 10 mm (right panel). doi:10.1371/journal.pone.0006175.g007 PLoS ONE | www.plosone.org 8 July 2009 | Volume 4 | Issue 7 | e6175