Gene Expression Profiles in Physic Nut Seeds Figure 5. Overview of expression profiles of genes involved in starch metabolism and reserve synthesis in developing physic nut seeds. A: Profiles of starch synthesis pathway genes. B: Profiles of a-amylase (AMY) and b-amylase (BAM) starch breakdown genes. C: Profiles of fatty acid synthesis pathway genes. D: Profiles of triacylglycerol assembly pathway genes. FAD2, oleate desaturase; FAD3, linoleate desaturase; FAH12, oleic acid hydroxylase; PDAT, phospholipid:diacylglycerol acyltransferase. E: Profiles of oil-body protein genes. F: Profiles of seed storage protein genes. 11S, homologs of 11S globulins; 2S, homologs of 2S albumins; 7S, homologs of 7S and 7S-like globulins. All expression data were transformed to log2 scale for plotting the profiles, and lines in different colors in A, C, and D indicate the expression patterns of different genes in the respective metabolic pathway. Values less than 22 indicate that no expression of the gene was detected at the indicated point. Genes shown in this figure are listed in Table S2. doi:10.1371/journal.pone.0036522.g005 the earliest stage, LEC2 and LEC1L genes were expressed from S3, while FUS3, ABI3, ABI4, and WRI1 genes were expressed from S4 (Figure 6). Discussion Studies on the regulation of physic nut lipid metabolism have important fundamental and practical applications. Here, we provide a systematic analysis of gene expression profiles in PLoS ONE | www.plosone.org 6 May 2012 | Volume 7 | Issue 5 | e36522