Eosinophil cdTCR Expression Figure 2. Surface expression of cdTCR/CD3 complex by human eosinophils. (A–C) CD3, cdTCR, Vc9, Vd2, Vd1, abTCR, CD8 and NKG2D surface expression on EPO+ (A) and MBP2+ (B) purified peripheral blood eosinophils analysed after 18 h culture and gated as described in Figure S1. Results in (A) and (B) represent to 2 distinct representative donors. Staining with control isotype antibodies is represented. (C) Presence of CD3+cdTCR+EPO+ and Vd1+d2+EPO+ eosinophils identified by triple staining. Staining with control isotype matched antibodies as well as corresponding double stainings is represented. doi:10.1371/journal.pone.0005926.g002 Figure 3. Heterogeneity of surface expression of cdTCR/CD3 complex by human eosinophils. (A) Cell surface marker expression on EPO+ gated peripheral blood eosinophils from 23 individual eosinophil donors. Group average expression for the indicated markers is represented (-). (B) Individual data and patient status. doi:10.1371/journal.pone.0005926.g003 PLoS ONE | www.plosone.org 5 June 2009 | Volume 4 | Issue 6 | e5926