p44 Functions in Prostate Epithelial Cells Figure 7. Temperature-sensitive large T antigen-immortalized prostate epithelial cells (LTAts-ECs) express markers of prostate epithelial cells. LTAts-ECs were grown at 33uC without (panels a-i) or with 10 nM DHT (panels j-l) and immunostained with anti-CK18, CK5, or AR antibody. Nuclei were stained with Sytox green. The inset in panel j shows AR expression in prostate epithelial cells detected by Western blotting with anti-AR antibody. doi:10.1371/journal.pone.0049173.g007 PLOS ONE | www.plosone.org 10 November 2012 | Volume 7 | Issue 11 | e49173