MoAbs to Toxic Gluten Peptides Figure 7. Indirect competitive ELISA using moAb A1 to test whole-wheat bread digests for 33-mer content. A. Concentration of 33-mer (mg/mL) in whole-wheat bread digests containing 0.6 mg/mL pepsin supplemented with specified concentrations of recombinant proEP-B2 (U/mg gluten). B. Concentration of 33-mer (mg/mL) in whole-wheat bread digests containing 0.6 mg/mL pepsin and 32 U/mg EP-B2 supplemented with specified concentrations of recombinant SC PEP (U/mg gluten). The concentration of 33-mer in each digest was determined by comparison to a synthetic 33-mer standard curve. Two separate assays were performed with the antibody, each with three repetitions. doi:10.1371/journal.pone.0002294.g007 PLoS ONE | www.plosone.org 9 May 2008 | Volume 3 | Issue 5 | e2294