Lifeact-mEGFP in Plant Cells Figure 5. High expression of Lifeact-mEGFP affects growth in moss protonemata. (A) Top panel shows protein extracted from indicated moss lines resolved by SDS-PAGE then immunoblotted with GFP specific antibodies. Coomassie stained RUBISCO large subunit (RBCL) is shown as loading control. The lower panel displays relative quantitation of immunoblot band intensity shown in top panel. (B) shows the results of a growth assay performed upon moss stably transformed with Lifeact-mEGFP lines. Top panel shows plant area as a fraction of the WT. Bottom panel shows solidity. Line 8 indicated by the asterisk is significantly different from WT and NLS4 for both area and solidity (ANOVA p,0.05). Variations in area for all other lines are not significant. (C) Three representative maximal projections of tip cells from line 22 (left) and line 8 (right). Bar is 5 mm. doi:10.1371/journal.pone.0005744.g005 PLoS ONE | www.plosone.org 7 May 2009 | Volume 4 | Issue 5 | e5744