OsLEA3-2 in Salt and Drought Tolerance Response Figure 4. Growth of S. cerevisiae cells expressing OsLEA3-2 and the control cell in the medium containing 1.2 M NaCl (A), 1.2 M KCl (B), and 2.0 M sorbitol (C). Yeast cells were grown on YNBG medium for two days. Twenty microliters of the culture was inoculated into YNBG supplemented with either 1.2 M NaCl, 1.2 M KCl, or 2.0 M sorbitol. At each time point, 60 microliters of culture was removed, and OD600 was measured with a spectrophotometer. doi:10.1371/journal.pone.0045117.g004 PLOS ONE | www.plosone.org 6 September 2012 | Volume 7 | Issue 9 | e45117