Identification of DAF-12 Target Genes by ChIP-chip Figure 2. Analysis of DAF-12 binding sites. (A) Distribution of the distance between DAF-12 binding sites and candidate gene targets (ATG). (B) Pie chart illustrates gene ontology for candidate target genes defined by biological function. (C) RNA pol II and DAF-12 binding sites in L2 stage animals were compared within the miRISC gene alg-2 using IGB software. A DR5 sequence within the DAF-12 binding peak is shown. (D) Analysis of the miRISC gene nhl-2. A total of 15 AGTGCA-related hexameric sequences were found. (E) Analysis of the miRISC gene ain-1. Multiple M-2 motifs were found. (F) AGTGCA-related sequences and M-2 motifs were over-represented among DAF-12 binding regions as identified by the MEME and DREME programs. The statistical significance of the motifs determined by p-values is also shown. doi:10.1371/journal.pgen.1002179.g002 PLoS Genetics | www.plosgenetics.org 5 July 2011 | Volume 7 | Issue 7 | e1002179