from EVTs or JEG-3 cells. GAPDH levels (C) provide PCR product standardization between lanes. L; DNA ladder. PCR reactions were performed on two separate occasions (N = 2). (EPS) Figure S3 ADAMTS-12 does not associate with the av integrin. av integrin was immunoprecipitated from protein lysates of JEG-3 cells stably transfected with LacZ (lanes 1 and 2), A12FL (lanes 3 and 4) or A12Mut (lanes 5 and 6). Immunopre- cipitates (IP) were probed for with antibodies directed against ADAMTS-12 (A) and av integrin (B). Representative autoradio- graphs from three independent experiments (N = 3) as described for A and B are shown. The molecular weight markers (kDa) are shown to the left. IB, immunoblot. (EPS) Table S1 Names of genes, DNA primer sequences and PCR conditions for the semiquantitative analysis of levels References 1. Aplin JD (1991) Implantation, trophoblast differentiation and haemochorial placentation: mechanistic evidence in vivo and in vitro. J Cell Sci 99: 681–92. 2. 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