19. Nagel SD, Boothroyd JC (1988) The alpha- and beta-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns. Mol Biochem Parasitol 29: 261–273. 20. Lupas A, Van Dyke M, Stock J (1991) Predicting coiled coils from protein sequences. Science 252: 1162–1164. 21. Melby TE, Ciampaglio CN, Briscoe G, Erickson HP (1998) The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge. J Cell Biol 142: 1595–1604. 22. Strunnikov AV, Larionov VL, Koshland D (1993) SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family. J Cell Biol 123: 1635–1648. 23. Hirano T, Mitchison TJ (1994) A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro. Cell 79: 449–458. 24. Saka Y, Sutani T, Yamashita Y, Saitoh S, Takeuchi M, et al. (1994) Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO J 13: 4938–4952. 25. Hirano T (2006) At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol 7: 311–322. 26. Losada A, Hirano T (2005) Dynamic molecular linkers of the genome: the first decade of SMC proteins. Genes Dev 19: 1269–1287. 27. Matsuyama A, Shimazu T, Sumida Y, Saito A, Yoshimatsu Y, et al. (2002) In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetyla- tion. EMBO J 21: 6820–6831. 28. Sasse R, Gull K (1988) Tubulin post-translational modifications and the construction of microtubular organelles in Trypanosoma brucei. J Cell Sci 90 (Pt 4): 577–589. 29. Westermann S, Weber K (2003) Post-translational modifications regulate microtubule function. Nat Rev Mol Cell Biol 4: 938–947. 30. Ems-McClung SC, Zheng Y, Walczak CE (2004) Importin alpha/beta and Ran- GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal. Mol Biol Cell 15: 46–57. 31. Mann T, Beckers C (2001) Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite Toxoplasma gondii. Mol Biochem Parasitol 115: 257–268. 32. Mann T, Gaskins E, Beckers C (2002) Proteolytic processing of TgIMC1 during maturation of the membrane skeleton of Toxoplasma gondii. J Biol Chem 277: 41240–41246. 33. Hu K, Mann T, Striepen B, Beckers CJ, Roos DS, et al. (2002) Daughter cell assembly in the protozoan parasite Toxoplasma gondii. Mol Biol Cell 13: 593–606. 34. Park RS, Legier MF, Cartwright J, Jr., Goldstein MA (1984) Perinuclear microtubules in postnatal rat heart. Journal of Morphology 179: 13–19. 35. Umeshima H, Hirano T, Kengaku M (2007) Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons. Proceedings of the National Academy of Sciences of the United States of America 104: 16182–16187. 36. Masson D, Kreis TE (1993) Identification and molecular characterization of E- MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells. Journal of Cell Biology 123: 357–371. Characterization of TgICMAP1 37. Hammond JW, Cai D, Verhey KJ (2008) Tubulin modifications and their cellular functions. Curr Opin Cell Biol 20: 71–76. 38. Liao G, Gundersen GG (1998) Kinesin is a candidate for cross-bridging microtubules and intermediate filaments. Selective binding of kinesin to detyrosinated tubulin and vimentin. J Biol Chem 273: 9797–9803. 39. Konishi Y, Setou M (2009) Tubulin tyrosination navigates the kinesin-1 motor domain to axons. Nat Neurosci 12: 559–567. 40. Peris L, Thery M, Faure J, Saoudi Y, Lafanechere L, et al. (2006) Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends. J Cell Biol 174: 839–849. 41. Reed NA, Cai D, Blasius TL, Jih GT, Meyhofer E, et al. (2006) Microtubule acetylation promotes kinesin-1 binding and transport. Curr Biol 16: 2166–2172. 42. Mital J (2006) Investigating the role of Apical Membrane Antigen 1 (TgAMA1) during Toxoplasma gondii invasion. Burlington, VT: University of Vermont. 43. Alexander DL, Mital J, Ward GE, Bradley P, Boothroyd JC (2005) Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles. PLoS Pathog 1: e17. 44. Kessler H, Herm-Gotz A, Hegge S, Rauch M, Soldati-Favre D, et al. (2008) Microneme protein 8—a new essential invasion factor in Toxoplasma gondii. J Cell Sci 121: 947–956. 45. Huynh MH, Rabenau KE, Harper JM, Beatty WL, Sibley LD, et al. (2003) Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complex. EMBO J 22: 2082–2090. 46. Hirokawa N (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279: 519–526. 47. Vale RD (2003) The molecular motor toolbox for intracellular transport. Cell 112: 467–480. 48. Tuma MC, Zill A, Le Bot N, Vernos I, Gelfand V (1998) Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores. J Cell Biol 143: 1547–1558. 49. Zhao C, Takita J, Tanaka Y, Setou M, Nakagawa T, et al. (2001) Charcot- Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta. Cell 105: 587–597. 50. Cassimeris L, Spittle C (2001) Regulation of microtubule-associated proteins. Int Rev Cytol 210: 163–226. 51. Roos DS, Donald RG, Morrissette NS, Moulton AL (1994) Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods Cell Biol 45: 27–63. 52. Guzman LM, Belin D, Carson MJ, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177: 4121–4130. 53. Desai A, Walczak CE (2001) Assays for microtubule-destabilizing kinesins. Methods Mol Biol 164: 109–121. 54. Hertzer KM, Ems-McClung SC, Kline-Smith SL, Lipkin TG, Gilbert SP, et al. (2006) Full-length dimeric MCAK is a more efficient microtubule depolymerase than minimal domain monomeric MCAK. Mol Biol Cell 17: 700–710. PLoS ONE | www.plosone.org 12 October 2009 | Volume 4 | Issue 10 | e7406