SC Dynamics at Steady State Figure 3. Sites of initial Zip1 entry into full-length SC localize near Zip3 foci. (A) Surface-spread meiotic nuclei from Zip3-MYC strains (K48) after a short induction of ZIP1-GFP expression (45 minutes). Zip3-MYC (red) foci often touch or overlap a Zip1-GFP (green) initial deposition event on full-length SCs (anti-Zip1 staining is shown in blue). Insets show 1.56 zoomed regions of the images, to highlight examples of Zip1-GFP localization with Zip3-MYC. Scale, 1 mm. (B) Bar graph indicating the percent of Zip1-GFP short stretches (0.35–0.5 mm) or foci that overlap or localize adjacent to (touching) a Zip3-MYC focus. The scatterplot in (C) displays the number of Zip3-MYC foci exhibited by synapsed chromosomes in nuclei prior to or after ZIP1-GFP induction. Circles indicate values for individual nuclei; horizontal lines indicate mean and standard error of the mean. doi:10.1371/journal.pgen.1002993.g003 PLOS Genetics | www.plosgenetics.org 6 October 2012 | Volume 8 | Issue 10 | e1002993