Sensory and Insulin-Like Signals Suppress Seizures Figure 4. The DAF-2/Insulin-like receptor suppresses sex-muscle excitability under food-deprived conditions. (A) Graph showing the effects of daf-2(lf) mutations on unc-103(0)-seizure susceptibility in food, inedible food, and no food conditions. The * indicates that the p-value is significantly different (p,0.05) compared to the unc-103(0) control condition (Fisher’s exact test). (B) Graph displaying the effect of ablating the AWC neurons in daf-2(e1368) unc-103(0) mutant males (p-value Fisher’s exact test). (C) Graph displaying the effect of AWC odorants (1024 isoamyl alcohol, 1027 2,3-pentanedione, and 1024 benzaldehyde) on unc-103(0)-induced seizures in unc-103(0) and daf-2(e1368) unc-103(0) males. Sephadex G-50 beads were used as a vehicle for the odorants (p-value Fisher’s exact test). (D) Temperature shift assay and heat-shock rescue for daf-2(e1368ts) unc- 103(0). Animals were grown at the restrictive temperature (25uC) and then food-deprived at either the restrictive or permissive temperature (15uC). For heat-shock rescue, L4 males were heat-shocked for 35 minutes prior to the assay. The p-values listed were calculated using Fisher’s exact test. doi:10.1371/journal.pgen.1000117.g004 PLoS Genetics | www.plosgenetics.org 8 July 2008 | Volume 4 | Issue 7 | e1000117