Found at: doi:10.1371/journal.pgen.1000639.s002 (0.48 MB TIF) Figure S3 DAF-16/FOXO nuclear localization in germline- ablated tcer-1(tm1452) mutants. Found at: doi:10.1371/journal.pgen.1000639.s003 (0.86 MB TIF) Figure S4 Expression of F52H3.5 in wild type, glp-1 mutant, and tcer-1; glp-1 mutant backgrounds. Found at: doi:10.1371/journal.pgen.1000639.s004 (2.08 MB TIF) Figure S5 Role of tcer-1 in the longevity of mutants in daf-16- independent pathways. Found at: doi:10.1371/journal.pgen.1000639.s005 (0.39 MB TIF) Table S1 Effect of tcer-1 RNAi and mutation on the lifespans of glp-1 mutants and wild-type worms. Found at: doi:10.1371/journal.pgen.1000639.s006 (0.15 MB PDF) Table S2 Effect of tcer-1 overexpression on the lifespan of wild- type animals, tcer-1 and long-lived mutants. Found at: doi:10.1371/journal.pgen.1000639.s007 (0.14 MB PDF) Table S3 Expression of DAF-16/FOXO-target genes in wild- type animals, glp-1 and daf-2 mutants. 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(1997) CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat- activated human immunodeficiency virus type 1 transcription. Mol Cell Biol 17: 6029–6039. PLoS Genetics | www.plosgenetics.org 12 September 2009 | Volume 5 | Issue 9 | e1000639