Vanin-1 in Vascular Remodeling Disease Figure 5. Activation of MMP-9 and migration are inhibited in Vnn12/2 SMCs. A, Serum-starved SMCs were treated with PDGF (10 ng/ml), cysteamine (500 ng/ml) or diamide (5 mM) for 48 h, and conditioned media analyzed for MMP-9 activity by gelatin zymography. B, Densitometric analysis of enhanced MMP-9 activity in WT compared to Vnn12/2 SMCs. Data pooled from 3 independent experiments for densitometry. C, We assayed migration in SMCs treated with diamide (5 mM) or PDGF (10 ng/ml), as described in the Methods. Total number of SMCs migrated/well after 48 h treatment with diamide or PDGF are shown. Data are mean 6 SD of 3 independent experiments. *P,0.05 vs. control. doi:10.1371/journal.pone.0039106.g005 PLoS ONE | www.plosone.org 8 June 2012 | Volume 7 | Issue 6 | e39106