BAX/BAK Independent Cell Death Figure 6. BH3 Peptides induces a decrease in mitochondrial membrane potential but does not engage the Permeability Transition Pore. (A and B) Quantification of TMRE release from wild type and Bax2/2/Bak2/2 MEFs respectively, as an output of mitochondrial membrane depolarization. Mitochondria were loaded with 50 nM TMRE and treated BID BH3 peptide (100 mM), mutant BID BH3 peptide (100 mM) or BIM BH3 PLoS ONE | www.plosone.org 8 May 2009 | Volume 4 | Issue 5 | e5646