The LARGE Principle in AFiPSCs Figure 3. Trophoblast differentiation of AFiPSCs. Exogenous activation of BMP signaling cascades and blocking of TGFbeta/Activin/Nodal signaling results in differentiation of AFiPSCs into the trophoblast lineage. (A) When AFiPSCs were treated with 100 ng/ml BMP2 or BMP4 for five days, a morphological change from densely packed colonies (A-I, -II, -V, -VI) towards cobblestone-like cell clusters (A-III, -IV, -VII, -VIII) was observed PLoS ONE | www.plosone.org 6 October 2010 | Volume 5 | Issue 10 | e13703