Anti-IGF-1R Potentiate the Effect of AS602868 Figure 5. AS602868+anti-IGF-1R antibody inhibit the NF-kB pathway in RPMI8226 and LP1 cells. 5.A AS602868 and anti-IGF-1R antibody decreased the level of NF-kB proteins pathways. RPMI8226 cells were treated for 16 h with 10 mg/mL anti-IGF-1R antibody or/and 10 mM AS602868 at 37uC. 5.B AS602868 and anti-IGF-1R antibody inhibited the expression of NF-kB regulated genes in multiple myeloma (IKB, IGF1, IL-6 and ICAM-1). Mean6 SD of three experiments ** P,0.001: * P,0.05 versus control. doi:10.1371/journal.pone.0022641.g005 Figure 6. Effect of AS602868 and anti-IGF-1R antibody on PI-3K/Akt pathway and proand anti-apoptotic proteins in RPMI8226 and LP1 cell lines. 6.A Anti-IGF-1R antibody inhibits the phosphorylation of Akt, p70S6K and 4EBP-1 in RPMI8226. 6.B Anti-IGF-1R antibody induces the expression of pro-apoptotic proteins. Cells were incubated for 16 h with 10 mg/mL anti-IGF-1R antibody or/and 10 mM AS602868. doi:10.1371/journal.pone.0022641.g006 PLoS ONE | www.plosone.org 6 July 2011 | Volume 6 | Issue 7 | e22641