Systems Biology Analysis of Chlorella vulgaris Figure 2. Workflow for de novo transcriptome assembly and comparative proteomic analyses. doi:10.1371/journal.pone.0025851.g002 Figure 3. Proteomic Analysis of C. vulgaris. (A) One dimensional SDS-PAGE of C. vulgaris soluble fraction utilized for comparative proteomic analysis. M, Marker; N+, Nitrogen-replete; N2, Nitrogen-deplete. (B) Mass spectral analysis data for the C. vulgaris proteome searched against all available Chlorophyta genome databases (left) and the C. vulgaris Transcriptome (right). doi:10.1371/journal.pone.0025851.g003 PLoS ONE | www.plosone.org 6 October 2011 | Volume 6 | Issue 10 | e25851