Lifeact-mEGFP in Plant Cells Figure 7. Latrunculin B-induced actin depolymerization alters localization of Lifeact-mEGFP signal. (A) Regenerating moss protoplasts were transferred to media containing increasing concentrations of Latrunculin B. After two days, solidity was measured and plotted versus the log of the concentration. The following are the IC50 values in mM calculated from these data: WT = 1.2160.07, Line 20 = 1.2660.03, Line 22 = 1.2360.12, Line 25 = 1.3460.06. (A total of 21–115 plants was analyzed per data point. ANOVA statistical analysis shows no significant differences.) (B) Moss cells expressing Lifeact-mEGFP, which have been subjected to increasing concentrations of Latrunculin B no longer show filamentous structures (bottom panel). Bar is 5 mm. (C) Lily pollen growing in control media reveals the cortical actin fringe. (D) After treatment with two 2 nM Latrunculin B, the filamentous signal has been lost and the fluorescence is now mostly cortical. Bar is 10 mm. See Movies S5 and S6 for complete series. doi:10.1371/journal.pone.0005744.g007 PLoS ONE | www.plosone.org 10 May 2009 | Volume 4 | Issue 5 | e5744