NQO1 Localizes to the Mitotic Spindle Figure 5. Co-localization of NQO1 and alpha-tubulin with mitotic spindles in human cells. Triple immunofluorescent staining for NQO1 (red, panel A); alpha-tubulin (green, panel B); merged (with DAPI,panel C). doi:10.1371/journal.pone.0044861.g005 were obtained from Cell Applications (San Diego, CA) and grown in Endothelial Cell Growth Media (Cell Applications). Primary human astrocytes were obtained from ScienCell Research Labs (Carlsbad, CA) and grown in Astrocyte Medium (ScienCell Research Labs). Immortalized human bone marrow endothelial cells (HBMEC) were provided by Dr. B.B. Weksler (Cornell University) and grown as previously described [36]. Immortalized human bronchial epithelial cells [37] (16HBE) were provided by Dr. Brian Day (National Jewish Health, Denver CO) and grown in RPMI 1640 containing 5% fetal bovine serum and 1% penicillin/ PLOS ONE | www.plosone.org 5 September 2012 | Volume 7 | Issue 9 | e44861