The Action Mechanism of Omomyc Figure 2. Omomyc intracellular co-localization with Miz-1 and c-Myc. A) Immunofluorescence photomicrographs of 293T cells transfected with Miz-1, c-Myc, FLAG-c-Myc and FLAG-Omomyc expressing plasmids - as indicated above panels - and stained with antibodies against FLAG (green), Miz-1 (red) and c-Myc (red) as indicated inside panels. Nuclei of the same fields were stained in blue with DAPI (4,6-diamidino-2- phenylindole). B) Quantitation of the experiments shown in A), based on the examination of 15 microscopy field and at least three biological replicates. doi:10.1371/journal.pone.0022284.g002 PLoS ONE | www.plosone.org 4 July 2011 | Volume 6 | Issue 7 | e22284