MU2 and Double Strand Break Repair Figure 1. Localization of MU2 protein. (A) Ovaries transgenic for mGFP-tagged MU2 were examined for mGFP fluorescence (green) and (A9) counterstained with DAPI. The oocyte nucleus and cytoplasm were fluorescent although the nucleus is brighter (arrowhead). (B) Testes from the transgenic animals were examined for mGFP fluorescence and (B9) DAPI staining. Fluorescence is not visible in the male germ cells, but is visible in somatic sheath cells. (C) Polytene chromosomes from salivary glands of transgenic animals carrying mGFP-tagged MU2 were examined for fluorescence. The localization of mGFP-MU2 was similar to the DAPI staining (C9). (D) Immmunostaining of Oregon R ovaries using anti-MU2 antiserum showed that MU2 is present primarily in the oocyte nucleus. The fluorescent signal in the oocyte nucleus may be more pronounced than in panel A. (E) mu2a oocytes immunostained with anti-MU2 antibody and (E9) with DAPI. The overall fluorescence intensity is much less than in panel D. (F) Mutant (mu2a) and wild type (OreR) larval (L) and the adult (A) extracts were prepared using RIPA buffer, and the MU2 protein in the extracts was detected using mouse anti-MU2 antiserum. doi:10.1371/journal.pgen.1000473.g001 PLoS Genetics | www.plosgenetics.org 3 May 2009 | Volume 5 | Issue 5 | e1000473