OsLEA3-2 in Salt and Drought Tolerance Response Figure 1. In silico analysis of the OsLEA3-2 protein. (A) Comparison of deduced amino acid sequence of OsLEA3-2 with OsLEA3 (AAD02421.1) and LEA3 proteins from A. thaliana (BAA11017.1) and B. napus (ACJ39155.1), underlines show the 11-mer repeats in the OsLEA3-2; (B) phylogenetic analysis of these group 3 LEA proteins; (C) hydropathy analysis of OsLEA3-2 protein. doi:10.1371/journal.pone.0045117.g001 Figure 2. OsLEA3-2 gene expression. (A) Expression profile of OsLEA3-2 in different tissues of Oryza sativa. Lane 1, embryo; lane 2, root; lane 3, shoot base; lane 4, leaf. (B) RT-PCR analysis of OsLEA3-2 gene expression in Oryza sativa under different stresses. Irrigation with half-strength Hoagland solution (A, control), 400 mM mannitol (B), sprayed with 100 mM ABA in 0.02% Tween-20 on seedlings (C), 4uC incubation (D), 200 mM NaCl (E), 25% PEG-6000 (F). 1, roots; 2, shoot base; 3, leaves. doi:10.1371/journal.pone.0045117.g002 PLOS ONE | www.plosone.org 4 September 2012 | Volume 7 | Issue 9 | e45117