IL-1b Production in Cystic Fibrosis Figure 1. Cell stimulation and inhibitor schedule. Schedule outlines the timing of inhibitor addition and priming in relation to inflammasome stimulation (t = 0) for THP-1 reporter and PBMC cytokine quantification experiments. Inhibitor treatments and stimulations were carried out as described in the Materials and Methods section. doi:10.1371/journal.pone.0037689.g001 Figure 2. Airway epithelial cells do not significantly contribute to IL-1b production in response to inflammasome stimuli. Control cell lines ((A) S9, (C) NuLi-1) and their corresponding CF cell lines ((B) IB3-1, and (D) CuFi-1) cells were stimulated with P. aeruginosa (MOI = 10), ATP (5 mM), or IL-1b (10 ng/ml), for the indicated times (n = 3 individual experiments). Cells were primed with LPS (100 ng/ml) for 4 hours where appropriate. Cell culture supernatants were assayed for IL-1b and IL-8 production by ELISA. Insert shows IL-8 secretion in response to stimulation with IL-1b (10 ng/ml). doi:10.1371/journal.pone.0037689.g002 PLoS ONE | www.plosone.org 3 May 2012 | Volume 7 | Issue 5 | e37689