Raf1 Is the DCAF of the CLRC Complex Figure 4. Heterochromatin, but not siRNA production, is erased in raf1-1 cells. (A) Swi6 localisation in indicated cells at 25u or 36uC. Representative images of fixed cells with Swi6 (green) and CENP-ACnp1(red). % cells with Swi6 localised at centromeres. (B) Northern: unprocessed otr transcripts in indicated cells at 25u or 36uC. Loading control: rRNA. (C) mat3-M:ura4+ silencing assay of cells grown at 36uC and shifted to 25uC (top PLoS Genetics | www.plosgenetics.org 8 February 2012 | Volume 8 | Issue 2 | e1002499