SOX9 Is an Activator and Repressor in Chondrocytes Figure 1. Conserved non-coding regions in COL10A1. (A) Global alignment of COL10A1 loci was performed using mammalian genomes. The translated regions in exons 2 and 3 of COL10A1 are highly conserved (deep blue). Element A of mouse Col10a1, which corresponds to the human enhancer, is located in the nearest conserved upstream non-coding region (pink). The untranslated regions (light blue) are poorly conserved in opossum. The mouse genome serves as the base sequence for alignment. Op: opossum; Ca: canine; Bo: bovine; Hu: human. (B) The 640 bp element A is located between 24286 and 23646 bp of mouse Col10a1. (C) Conserved non-coding regions in mammalian COL10A1. Alignment showed that the functional SOX9 binding site (COL2C1) is conserved in the mammalian COL10A1 enhancers together with the adjacent TG-rich sequence. The PLoS Genetics | www.plosgenetics.org 3 November 2011 | Volume 7 | Issue 11 | e1002356