GASZ Is Essential for Male Meiosis Figure 3. GASZ co-immunolocalization with intermitochondrial cement markers. (A–F) Immunofluorescent analysis of spermatocytes (A– C) and spermatids (D–F). (A,D) GASZ (red) localizes between mitochondrial clusters in spermatocytes using antibodies to cytochrome c (green). (A inset) Higher magnification. (B,E) Staining is shown for GASZ (red), TDRD1 (green). (C,F) Staining for GASZ (red) and MVH (green). Arrows in (E,F) identify the chromatoid body. Note the presence of the GASZ (red) foci and corresponding TDRD1 and MVH (green) foci in spermatocytes (A–C) but not in spermatids (D–F). (G–I) Staining is shown for GASZ (G), TDRD1 (H), and merged (I). (J–L) Staining is shown for GASZ (J), MILI (K), and merged (L). (M–O) Staining of newborn testes is shown for GASZ (M), MILI (N), and merged (O). Note the presence of the GASZ (green) foci and corresponding MILI (red) foci in gonocytes (arrowheads). [Scaling: 10,0006(A–F), 40X(G–L), and 4006(M–O) magnification.] doi:10.1371/journal.pgen.1000635.g003 PLoS Genetics | www.plosgenetics.org 5 September 2009 | Volume 5 | Issue 9 | e1000635