Environmental stress Eggs from WT or daf-12(0) worms carrying the SCM::GFP transgene were obtained by hypochlorite treatment and incubated for 72 hours at 20uC or 15uC–25uC cycles of 4 hours each with a programmable incubator (Innova 4230, New Brunswick Scientific, New Brunswick, NJ). Supporting Information Figure S1 Enriched DAF-12 binding sites were analyzed by (A) DREME or (B) MEME. (PDF) Comparison between PHA-4 and DAF-12 target DAF-12 binding sites at the lin-42 locus. Heterochronic phenotypes enhanced by daf-12(0). Figure S5 Mutation of M-2 and DR domains (highlighted) within lin-42A promoter. Mutations are shown above the WT sequence. (PDF) Heterochronic phenotypes suppressed by dauer Dataset S1 (PDF) Figure S2 genes. (EPS) Figure S3 (EPS) Figure S4 (PDF) Figure S6 formation. (PDF) References 1. Antebi A, Culotti JG, Hedgecock EM (1998) daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191–1205. 2. 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Zhang Y, Nash L, Fisher AL (2008) A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering. BMC Dev Biol 8: 119. PLoS Genetics | www.plosgenetics.org 15 July 2011 | Volume 7 | Issue 7 | e1002179