Ethical oversight Bone-marrow derived macrophages used in this study were previously derived from B10A.Bcgr mice [32]. Experiments done on the animals used in that study [32] adhered to the McGill University’s guidelines for animal husbandry and was approved by the institutional research ethics committee. The current study did not require any ethical approval from the review board since it did not involve any animal work or human-derived cells. Supporting Information Figure S1 Flowcytometry analysis of cell damage during temperature shift. Stationary L. mexicana parasites were stained with PI to measure the percentage of damaged cells after 4 h of Temperature shift (TS). Percentage of PI-positive cells remains negligible following 4 h of TS. However, addition of hydrogen peroxide together with TS induces cell damage. Nil represents stationary parasites before washing with PBS (refer to materials and methods). Blue: Non-stained cells, Red: stained cells. (TIF) Table S1 Full list of identified proteins that are secreted within 4 hours of temperature shift. Proteins that are being reported for the first time are marked with an asterisk. Accession numbers belong to Protein Databank of NCBI. (XLS) Alternative Language Abstract S1 Farsi (Persian) trans- lation provided by Kasra Hassani. (PDF) Alternative Language Abstract S2 provided by Amandine Isnard. (PDF) Alternative Language Abstract S3 provided by Cecilia Quiroga. (PDF) References French translation Spanish translation 1. Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, et al. (2007) Cutaneous leishmaniasis. Lancet Infect Dis 7: 581–596. 2. Olivier M, Gregory DJ, Forget G (2005) Subversion mechanisms by which Leishmania parasites can escape the host immune response: a signaling point of view. Clin Microbiol Rev 18: 293–305. 3. Abu-Dayyeh I, Shio MT, Sato S, Akira S, Cousineau B, et al. (2008) Leishmania-Induced IRAK-1 Inactivation Is Mediated by SHP-1 Interacting with an Evolutionarily Conserved KTIM Motif. PLoS Negl Trop Dis 2: e305. 4. Gregory DJ, Godbout M, Contreras I, Forget G, Olivier M (2008) A novel form of NF-kappaB is induced by Leishmania infection: involvement in macrophage gene expression. Eur J Immunol 38: 1071–1081. 5. Gomez MA, Contreras I, Halle M, Tremblay ML, McMaster RW, et al. (2009) Leishmania GP63 alters host signaling through cleavage-activated protein tyrosine phosphatases. Sci Signal 2: ra58. 6. Thery C, Ostrowski M, Segura E (2009) Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 9: 581–593. 7. Corrales RM, Sereno D, Mathieu-Daude F (2010) Deciphering the Leishmania exoproteome: what we know and what we can learn. FEMS Immunol Med Microbiol 58: 27–38. 8. Cuervo P, De Jesus JB, Saboia-Vahia L, Mendonca-Lima L, Domont GB, et al. (2009) Proteomic characterization of the released/secreted proteins of Leishmania (Viannia) braziliensis promastigotes. J Proteomics 73: 79–92. 9. Silverman JM, Chan SK, Robinson DP, Dwyer DM, Nandan D, et al. (2008) Proteomic analysis of the secretome of Leishmania donovani. Genome Biol 9: R35. 10. Geiger A, Hirtz C, Becue T, Bellard E, Centeno D, et al. (2010) Exocytosis and protein secretion in Trypanosoma. BMC Microbiol 10: 20. 11. Silverman JM, Clos J, de’Oliveira CC, Shirvani O, Fang Y, et al. (2010) An exosome-based secretion pathway is responsible for protein export from Leishmania and communication with macrophages. J Cell Sci 123: 842–852. 12. Trocoli Torrecilhas AC, Tonelli RR, Pavanelli WR, da Silva JS, Schumacher RI, et al. (2009) Trypanosoma cruzi: parasite shed vesicles Secreted Proteins of Leishmania Alter Macrophage Alternative Language Abstract S4 provided by Felix Hugentobler. (PDF) Alternative Language Abstract S5 provided by Marina T. Shio. (PDF) German translation Portuguese translation Alternative Language Abstract S6 Amharic (Ethiopian) translation provided by Fikregabrail Aberra Kassa. (PDF) Alternative Language Abstract S7 provided by Issa Abu-Dayyeh. (PDF) Arabic translation File S1 Spectrum, Spectrum/Model error and Frag- mentation table of proteins identified by a single peptide. (PDF) Acknowledgments Proteomic analyses were performed on the Proteomics Platform of the McGill University and Genome Que´bec Innovation Centre, Montre´al, Canada. SEM was performed in FEMR center at McGill University, Montre´al, Canada. The authors thank Dr. Marie-Jose´e Bellemare for assistance with SEM, Dr. Sylvie Laboissiere, Mr. Fikergabrail Kassa and Mr. Yovani Moreno for their comments on the proteomic analyses. 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