staining with ponceau S. Lane2, Western blotting analysis of VER2 in wheat plants vernalized for 3 weeks. Found at: doi:10.1371/journal.pone.0004854.s001 (8.56 MB TIF) Figure S2 Organ-specific accumulation of VER2 protein during heading stage in winter wheat. (A) Heading stages for analysis. S1, before heading stage; S2, during heading stage. Corresponding panicles were shown below. (B) Expression patterns and abundance of VER2 during different development stages of panicle. Expression level of tubulin detected by immunoblotting was used as loading control. Found at: doi:10.1371/journal.pone.0004854.s002 (2.82 MB TIF) References 1. Peumans WJ, Hause B, Van Damme EJ (2000) The galactose-binding and mannose-binding jacalin-related lectins are located in different sub-cellular compartments. FEBS Lett 477: 186–192. 2. 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