CD69 Role on T Cell Priming Figure 2. CD69 is upregulated on BMDC upon activation. CD69+/+ or CD692/2 GM-CSF BMDC were treated with LPS at different conditions of time and dose. Subsequently they were stained for CD11c and CD69, and analyzed by flow cytometry. A. Overlay of CD69 histograms of CD69+/+ untreated cells (dashed line), and CD69–/2 (grey filled) and CD69+/+ (solid line) cells cultured for 12h with 1 mg/ml of LPS (right). B. Time course of CD69 upregulation in response to 1 mg/ml of LPS. CD69 expression is the difference in MFI between CD69+/+ and CD692/2 BMDC. C. CD69+/+ BMDC were cultured for 24h with growing doses of LPS and with anti-CD69 2.2 (to provide a background staining control) or Isotype control. CD69 surface expression is expressed as the difference in MFI between unblocked and blocked samples. Results are representative of two experiments with similar PLOS ONE | www.plosone.org 3 October 2012 | Volume 7 | Issue 10 | e48593