Locostatin Impairs Migration Figure 4. Locostatin impairs wound healing in wild-type and depleted RKIP MEFs. MEFs expressing wild-type (A, B) or depleted (C, D) RKIP were seeded at 0.56106/ml and grown for 24 h before treatment with either DMSO (control) or locostatin at 20 (A) and (C) or 50 mM (B) and (D). (E) A standardized scratch (wound) was applied to monolayers and digital images were taken at time points indicated. Cell migration was determined as described in materials and methods. Data represents the mean6SEM of eight wounds from two independent experiments. *p,0.05, **p,0.01 indicates the significance of the change relative to the corresponding sample in the absence of locostatin. doi:10.1371/journal.pone.0006028.g004 PLoS ONE | www.plosone.org 7 June 2009 | Volume 4 | Issue 6 | e6028