The CD1 System in Humanized Mice Figure 6. Detection of iNKT cells. A) Flow cytometric analysis of splenocytes from an engrafted mouse using fluorescently labeled human CD1d tetramer (y-axis) and an antibody against human CD3 (x-axis). The left plot shows staining using vehicle treated CD1d tetramer, and the right plot shows a-GalCer loaded CD1d tetramer. B) Percentages of CD1d tetramer-positive cells detected from splenocytes of 10 engrafted mice, compared to PBMCs from 15 healthy adult human donors. The medians for each data group are as follows: spleen cells from hu-NSG mice, tetramer+vehicle = 0.0039%, tetramer+a-GalCer = 0.048%; human PBMCs, CD1d tetramer+vehicle = 0.0041%, CD1d tetramer+a-GalCer = 0.0745%. C) Analysis of the splenocytes and liver mononuclear cells from 4 engrafted mice for human cells stained by CD3 and a-GalCer loaded CD1d tetramer. doi:10.1371/journal.pone.0021701.g006 PLoS ONE | www.plosone.org 11 June 2011 | Volume 6 | Issue 6 | e21701