23. Blanchette J, Racette N, Faure R, Siminovitch KA, Olivier M (1999) Leishmania-induced increases in activation of macrophage SHP-1 tyrosine phosphatase are associated with impaired IFN-gamma-triggered JAK2 activa- tion. Eur J Immunol 29: 3737–3744. 24. Contreras I, Go´mez MA, Nguyen O, Shio MT, McMaster RW, et al. (2010) Leishmania-Induced Inactivation of the Macrophage Transcription Factor AP-1 Is Mediated by the Parasite Metalloprotease GP63. PLoS Pathog 6: e1001148. 25. Heinonen KM, Dube N, Bourdeau A, Lapp WS, Tremblay ML (2006) Protein tyrosine phosphatase 1B negatively regulates macrophage development through CSF-1 signaling. Proc Natl Acad Sci U S A 103: 2776–2781. 26. Xu H, An H, Hou J, Han C, Wang P, et al. (2008) Phosphatase PTP1B negatively regulates MyD88- and TRIF-dependent proinflammatory cytokine and type I interferon production in TLR-triggered macrophages. Mol Immunol 45: 3545–3552. 27. Blanchette J, Pouliot P, Olivier M (2007) Role of protein tyrosine phosphatases in the regulation of interferon-{gamma}-induced macrophage nitric oxide generation: implication of ERK pathway and AP-1 activation. J Leukoc Biol 81: 835–844. 28. Forget G, Gregory DJ, Whitcombe LA, Olivier M (2006) Role of host protein tyrosine phosphatase SHP-1 in Leishmania donovani-induced inhibition of nitric oxide production. Infect Immun 74: 6272–6279. 29. Lapara NJ, 3rd, Kelly BL (2010) Suppression of LPS-induced inflammatory responses in macrophages infected with Leishmania. J Inflamm (Lond) 7: 8. 30. Silverman JM, Clos J, Horakova E, Wang AY, Wiesgigl M, et al. (2010) Leishmania Exosomes Modulate Innate and Adaptive Immune Responses through Effects on Monocytes and Dendritic Cells. J Immunol. Secreted Proteins of Leishmania Alter Macrophage 31. Bendtsen JD, Jensen LJ, Blom N, Von Heijne G, Brunak S (2004) Feature-based prediction of non-classical and leaderless protein secretion. Protein Eng Des Sel 17: 349–356. 32. Radzioch D, Hudson T, Boule M, Barrera L, Urbance JW, et al. (1991) Genetic resistance/susceptibility to mycobacteria: phenotypic expression in bone marrow derived macrophage lines. J Leukoc Biol 50: 263–272. 33. Robinson JP (1998) Current protocols in cytometry. New York: Wiley. 34. Wasiak S, Legendre-Guillemin V, Puertollano R, Blondeau F, Girard M, et al. (2002) Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics. J Cell Biol 158: 855–862. 35. Keller A, Nesvizhskii AI, Kolker E, Aebersold R (2002) Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem 74: 5383–5392. 36. Gotz S, Garcia-Gomez JM, Terol J, Williams TD, Nagaraj SH, et al. (2008) High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res 36: 3420–3435. 37. Mulder NJ, Apweiler R, Attwood TK, Bairoch A, Bateman A, et al. (2007) New developments in the InterPro database. Nucleic Acids Res 35: D224–228. 38. Myhre S, Tveit H, Mollestad T, Laegreid A (2006) Additional gene ontology structure for improved biological reasoning. Bioinformatics 22: 2020–2027. 39. Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340: 783–795. 40. Markova B, Gulati P, Herrlich PA, Bohmer FD (2005) Investigation of protein- tyrosine phosphatases by in-gel assays. Methods 35: 22–27. PLoS ONE | www.plosone.org 10 May 2011 | Volume 6 | Issue 5 | e18724