Detection of Receptor Activation in FFPE Specimens Figure 7. VeraTag FFPE quantification of HGF/c-MET ligand-receptor complexes and correlation with c-MET (Tyr1003) phosphorylation. Ai. Schematic of the HGF/c-MET VeraTag FFPE proximity assay. Aii. Quantification of HGF/c-MET levels in unstimulated and HGF stimulated A549 cells. Isotype IgG control signals (inset) were subtracted from the HGF/c-MET assay signals. B. VeraTag quantification of HGF/c- PLoS ONE | www.plosone.org 11 January 2011 | Volume 6 | Issue 1 | e15932