Clr-C Required for Heterochromatin Establishment Figure 4. Components of the Clr-C complex are required for establishment of H3K9me2 at centromeres. A. ChIP analysis of H3K9me2 at dh repeats compared with adh1+ locus measured by real time PCR. Enrichment of H3K9me2 was normalized to a strain lacking clr4+. Data represents the mean of 2 independent ChIP experiments 6 SEM. Strains analyzed PY2036, 1838, 3494, 3497, 3293, 3781, 3783, 3791, 3792. B. ChIP analysis of PLoS Genetics | www.plosgenetics.org 8 October 2010 | Volume 6 | Issue 10 | e1001174