NKX2-5 GATA4 MEF2c aMHC cTnT cTnI ISL-1 ANF HAND2 TBX2 HAND1 TBX5 Effectors Wnt1 Wnt2 Wnt2B Wnt5A Wnt5B Wnt8B Wnt11 Activin A receptor N-cadherin 2 DKK2 DKK3 Frizzled 4 Frizzled 9 Frizzled 10 LEF1 NFATC2 NFATC4 RARb Inhibitors PPP2CA PPP2R1B PPP2R2B SFRP1 SFRP2 SFRP3 SOX2 SOX3 SOX11 tube tube loop loop contract contract A/RV A RV AV LV LV *A, atrium; RV, right ventricle; AV, atrioventricular septum; LV, left ventricle. **FDR, false discovery rate. doi:10.1371/journal.pone.0016004.t001 Table 2. Fold-changes in Wnt Pathway Genes. Wnt Genes Day 8 v. Day 0 [FDR]* Day 14 v. Day 0 [FDR] 17 47 - - 4 - 8 4 4 - 5 4 5 - 15 4 - - 22 25 29 23 219 - 217 212 23 [0.001] [0] - - [0.005] - [0.007] [0.011] [0.042] - [0.002] [0.018] [0.012] - [0.001] [0.004] - - [0.045] [0.013] [0.002] [0.017] [0.006] - [0.001] [0.003] [0.018] 32 26 3 5 5 4 10 4 - 5 7 - - 3 20 5 3 3 26 24 26 - 27 5 29 25 - [0] [0] [0.039] [0.009] [0.002] [0.041] [0.005] [0.007] - [0.022] [0.001] - - [0.017] [0] [0.002] [0.011] [0.045] [0.046] [0.027] [0.003] - [0.001] [0.029] [0.002] [0.016] - 34 24 28 841 - - 4 82 232 - - - [0.002] [0] [0] [0] - - [0.012] [0] [0] - - - TGFb Genes Effectors TGFb2 TGFb3 Activin/Inhibin A Activin A receptor BMP2 BMP4 BMP5 BMP7 BMP10 SMAD3 SMAD6 SMAD9 Id2 Id3 Id4 Inhibitors MAP3K7 MAPK1 MAPK8 c-Myc DP1 E2F4 31 4 3 4 16 6 65 8 14 - 4 4 7 4 12 22 23 22 213 22 23 [0] [0.043] [0.013] [0.011] [0] [0.013] [0] [0.003] [0.013] - [0.019] [0.007] [0.003] [0.012] [0.001] [0.038] [0.038] [0.027] [0.001] [0.050] [0.028] 42 29 26 886 122 32 16 317 476 46 33 74 51 - - 4 18 25 135 10 - 2 - 8 - - - - - - 210 23 23 [0.001] [0] [0] [0] [0] [0] [0.001] [0] [0] [0] [0] [0] [0] - - [0.007] [0] [0.001] [0] [0.002] - [0.022] - [0.001] - - - - - - [0.001] [0.017] [0.020] Selection of Human Myocardial Precursors Table 1. Fold-changes in Cardiac-Specific Genes. Cardiac Genes Stage/Chamber* Day 8 v. Day 0 [FDR**] Day 14 v. Day 0 [FDR] Plasmid and cell line construction The 2K7bsd lentivector (kindly provided by David Suter [14]) was used to assemble a lentiviral plasmid capable of driving the expression of enhanced green fluorescent protein (GFP) by a ubiquitin-C or a-myosin heavy chain (aMHC) promoter. The Table 3. Fold-changes in TGFb Pathway Genes. Day 8 v. Day 0 [FDR*] Day 14 v. Day 0 [FDR] *FDR, false discovery rate. doi:10.1371/journal.pone.0016004.t002 *FDR, false discovery rate. doi:10.1371/journal.pone.0016004.t003 PLoS ONE | www.plosone.org 8 January 2011 | Volume 6 | Issue 1 | e16004