Study Human Chemokine Receptor Figure 6. Detergent screening for solubilization of Trx-hCRs expressed by pBAD-hCRs in Top10. A)–D) Effectiveness of the 96 detergents on solubilizing Trx-hCRs from cell lysates, as detected by Dot blot. Immunoblotting analysis was performed using mouse anti-His6-tag monoclonal antibody. Each filter has 96 spots (a1 to h12), corresponding to the 96 detergents listed in Figure 5. Controls shown in (A) are positive control (+) using 1:100 dilution of BenchMark His-tagged protein standard; negative control (21) was pBAD-hCCR5 in Top10 cultured in the absence of inducer; negative control (22) was L-arabinose induced sample solubilized without the addition of detergent. The negative controls were processed in PLoS ONE | www.plosone.org 9 February 2009 | Volume 4 | Issue 2 | e4509