Locostatin Impairs Migration Figure 5. Locostatin impairs wound healing independent of RKIP. MEFs expressing wild-type (A, B) or deficient (C, D) RKIP were seeded at 0.56106/ml and grown for 24 h before treatment with either DMSO (control) or 20 (A) and (C) or 50 mM (B) and (D) locostatin. (E) A standardized scratch (wound) was applied to monolayers and analyzed as in materials and methods. Data represents the mean6SEM of three independent experiments (n = 4 wounds/sample). *p,0.05, **p,0.01 indicates the significance of the change relative to the corresponding sample in the absence of locostatin. doi:10.1371/journal.pone.0006028.g005 PLoS ONE | www.plosone.org 8 June 2009 | Volume 4 | Issue 6 | e6028