Secretional Blockade by VSL#3 Figure 1. L. casei selectively inhibits TNF-induced secretion of chemotactically functional IP-10. Mode-K cells were stimulated for 24 h with (A) VSL#3 (moi 20) or (B) L. casei (moi 20) with or without TNF (10 ng/ml) activation for 24 h. The concentration of IP-10 and IL-6 in cell culture supernatants was measured by ELISA. Bars represent mean values (+/2 SD) of triplicate samples. (C) 100 ml DMEM or cell culture supernatant from a 24 h stimulation experiment with Mode-K cells (control, TNF (10 ng/ml), TNF+L. casei (moi 20)) were added to 500 ml of transmigration medium. The amount of transmigrated cells per region of interest was determined by axiovision cell counting. Pictures show representative sections of transmigrated cells of three independent experiments and bars represent mean values (+/2 SD) of triplicate samples. doi:10.1371/journal.pone.0004365.g001 PLoS ONE | www.plosone.org 4 February 2009 | Volume 4 | Issue 2 | e4365