Spatial Segregation of BMP/Smad Signaling Figure 3. Gene expression is differentially affected by inhibition of dynamin-dependent endocytosis. Serum-starved C2C12 cells were treated for 2 h with 40 mM dynasore or 0.05% DSMO prior to stimulation with 30 nM BMP-2 for 6 h in medium containing dynasore or DMSO. RNA was isolated and subjected to whole genome profiling using the Illumina BeadChip system. Expression profiles were analyzed relative to DMSO control treatment. (A) Venn diagram based on significantly detected genes (1.4 fold regulation, detection P-value,0.05) (the entire gene list is depicted in Table S2). (B) Heatmap depicting a subset of BMP-2-induced genes, which are endocytosis-dependent (the complete list is given in Table S3). (C) Heatmap depicting BMP-2-induced genes which are endocytosis-independent (the gene list is shown in Table S4). (B,C) The heatmaps are colored by log2 expression signals according to the color key at the bottom. (D) Selected genes from lists representing endocytosis-dependent or - independent genes displayed in Table S3 and S4, respectively. doi:10.1371/journal.pone.0025163.g003 PLoS ONE | www.plosone.org 5 October 2011 | Volume 6 | Issue 10 | e25163