NSAID and Gluten Sensitivity Figure 5. Splenocyte proliferation after incubation with PT gliadin. Proliferation was measured by 3H-thymidine incorporation and expressed as stimulation index. Splenocytes from gluten-sensitized mice treated with or without indomethacin exhibited increased proliferation compared to non-sensitized controls. Data represent the means6SEM of 6 mice/group. doi:10.1371/journal.pone.0006472.g005 Figure 6. Cytokines in supernatant of splenocyte cultures after incubation with PT-gliadin (black) or medium (white). Expressions of (A) IL-12p70, (B) IFN-c, (C) IL-10 were determined by CBA analysis. Culture supernatants from gluten-sensitized plus indomethacin (Indo) treated mice showed increased IFN- c (*p,0.01 vs all groups). Cultured splenocytes from gluten-sensitized mice, with or without indomethacin showed a trend for increased IL-10 release after PT-gliadin stimulation (p = 0.09). Data represent the means6SEM of 6 mice/group. doi:10.1371/journal.pone.0006472.g006 PLoS ONE | www.plosone.org 5 July 2009 | Volume 4 | Issue 7 | e6472