Retrieval of V-ATPase 46. Rauchenberger R, Hacker U, Murphy J, Niewohner J, Maniak M (1997) Coronin and vacuolin identify consecutive stages of a late, actin-coated endocytic compartment in Dictyostelium. Curr Biol 7: 215–218. 47. Insall R, Mu¨ller-Taubenberger A, Machesky L, Ko¨hler J, Simmeth E, et al. (2001) Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motil Cytoskeleton 50: 115–128. 48. Lee E, Knecht DA (2002) Visualization of actin dynamics during macro- pinocytosis and exocytosis. Traffic 3: 186–192. 49. Lukacs GL, Rotstein OD, Grinstein S (1991) Determinants of the phagosomal pH in macrophages. In situ assessment of vacuolar H(+)-ATPase activity, counterion conductance, and H+ ‘‘leak’’. J Biol Chem 266: 24540–24548. 50. Steinberg BE, Touret N, Vargas-Caballero M, Grinstein S (2007) In situ measurement of the electrical potential across the phagosomal membrane using FRET and its contribution to the proton-motive force. Proc Natl Acad Sci U S A 104: 9523–9528. 51. Aubry L, Klein G, Martiel JL, Satre M (1993) Kinetics of endosomal pH evolution in Dictyostelium discoideum amoebae. Study by fluorescence spectroscopy. J Cell Sci 105 (Pt 3): 861–866. 52. Jenne N, Rauchenberger R, Hacker U, Kast T, Maniak M (1998) Targeted gene disruption reveals a role for vacuolin B in the late endocytic pathway and exocytosis. J Cell Sci 111 (Pt 1): 61–70. 53. Maniak M (2001) Fluid-phase uptake and transit in axenic Dictyostelium cells. Biochim Biophys Acta 1525: 197–204. 54. Recchi C, Chavrier P (2006) V-ATPase: a potential pH sensor. Nat Cell Biol 8: 107–109. 55. Hurtado-Lorenzo A, Skinner M, El Annan J, Futai M, Sun-Wada GH, et al. (2006) V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway. Nat Cell Biol 8: 124–136. 56. Heuser J (2006) Evidence for recycling of contractile vacuole membrane during osmoregulation in Dictyostelium amoebae–a tribute to Gunther Gerisch. Eur J Cell Biol 85: 859–871. PLoS ONE | www.plosone.org 14 January 2010 | Volume 5 | Issue 1 | e8585