Rad51 Targeted Adenoviral Vector Figure 2. Identification of a core Rad51 promoter element. (A) Luciferase assays measuring Rad51 promoter activity in 9 different cancer and non-cancer cell lines. Cells were transduced with their respective vectors at a MOI of 100. 24 hours post-infection, cells were lysed and luciferase activity of the cell lysates measured. Data are presented as mean values of independent experimental triplicates; error bars represent the standard deviation of the data values. (B) As a measure of tumor specificity, luciferase activity for each construct in each cell type was calculated as a ratio relative to its activity in normal lung fibroblasts (Wi-38). doi:10.1371/journal.pone.0028714.g002 PLoS ONE | www.plosone.org 4 December 2011 | Volume 6 | Issue 12 | e28714