pioneers and hspb11 expression is absent. (C) Homozygous sopfixe2/2 mutants show wildtype eng2a expression in muscle pioneers and at the midbrain-hindbrain boundary (mhb). (D and E) Muscle pioneer development in MO(UTR)-hspb11 and mmMO-hspb11 injected embryos was not effected, confirmed by eng2a expression. (TIF) Figure S6 MO(ATG)-hspb11 and MO(59UTR)-hspb11 morpholinos specifically target their hspb11 binding sites. Embryos injected with gfp mRNA that contains the MO binding site expresses GFP. Co-injection of MO(ATG)-hspb11 or MO(59UTR)-hspb11 with the gfp mRNA abolishes GFP expres- sion, whereas with the mmMO-hspb11 morpholino does not reduce GFP expression. atg-gfp mRNA contains MO(ATG)-hspb11 binding site. utr-gfp mRNA contains MO(UTR)-hspb11 binding site. (TIF) Table S1 Gene sets exhibiting greatest overlap to differentially expressed genes in APM treated zebrafish embryos. The analysis was performed by the software GSEA References 1. 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