Table 1. Primer sequences for real-time PCR. Gene DLL4 Efnb2 Nr2f2 Jag1 Notch4 EphB4 Forward primer Reverse primer TAC TGC TGG TGT TGC TGG TC GCA GCA GGG ATT AGG TTG TC AGA CAC CGC AAA CAC TCT CC CGT AGT GTG GGC AGA AGA CA AAA GTC CCA GTG TGC TTT GG CCT ACC AAA CGG ACG AAA AA TCT ACT GGT GTG TGC GGA AG AAT CCT TGA TGG GGA CTG TG GTG GAG GAC CTG GTT GAA GA GCA TCT TTA TCC GCT CCA GT TGA AGG GTA CCT GGT TCG AC TTC CCT CCA CTC ACA ACA CA Dynactin1 CGA GAA GCT CAA GGA TGA GG GAA GGT CAC TTT GCC CAT GT doi:10.1371/journal.pone.0027332.t001 Real-time PCR analysis Sybr-green-based, real-time PCR was performed to determine the relative expression of transcripts (rat Dll4, Efnb2, Nr2f2, Jag1, Notch4 and Ephb4). 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