Detection of Receptor Activation in FFPE Specimens Figure 8. Measurement of the HGF/c-MET complex by Surface Protein-Protein Interaction by Cross-linking ELISA (SPPICE) assay. A. SPPICE schemetic. c-MET was chemically cross-linked to expgenous HGF using the membrane impermeable sulfo-EGS cross-linker. Membrane proteins were solublized and HGF/c-MET complexes were measured using the SPPICE procedure (Bi) or by immunoprecipitation with HGF antibodies PLoS ONE | www.plosone.org 13 January 2011 | Volume 6 | Issue 1 | e15932