Figure S3 Ectopic expression of DRP-1, ZIPk and DRP- 1b induces LC3 shift. HEK293T cells were transfected with the FLAG-tagged ZIPk, DRP-1b or DRP-1 expression vectors or were mock transfected with a nonrelevant protein expressing plasmind, and were harvested 24 h post transfection. Lysates were immunoblotted using anti-LC3, anti-FLAG and anti-Actin antibodies. (TIF) Figure S4 The binding of DRP-1b to ATF-4 is through the leucine zipper-like domain. HEK293T cells were co- transfected with the indicated vectors and harvested 24 h post transfection. Lysates were immunoprecipitated using anti-FLAG antibodies, and protein levels were detected using western blot with the indicated antibodies. (TIF) Table S1 Sequences used in the multiple sequence alignment of the DAP kinases. The table details organisms, References 1. Bialik S, Kimchi A (2006) The death-associated protein kinases: structure, function, and beyond. Annu Rev Biochem 75: 189–210. 2. 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