Exercise and Leptin Action Figure 7. Leptin effects on 12-h cumulative food intake and AMPK/mTOR signaling in the hypothalami of control, DIO and exercised DIO rats. (a) Representative Western blots of five independent experiments showing hypothalamic IL-6 expression in DIO Wistar rats at rest and immediately after acute exercise. (b) Leptin (1026 M) was administered in control, DIO and exercised DIO rats. Animals were immediately exposed to food for a 12-hour period (n = 12–15 animals per group). (c, d, e and f) Representative Western blots of five independent experiments showing hypothalamic lysates from Wistar rats. Phospho-AMPKthr172, threonine-phosphorylated AMPK and total AMPK (c); phospho-ACCser79, serine phosphorylated ACC and total ACC (d); phospho-p70S6Kinasethr389, threonine phosphorylated p70S6Kinase and total p70S6K (e); phospho-4EBP1thr70, threonine phosphorylated 4EBP1 and total 4EBP1 (f). Data are the means6SEM. * p,0.05, vs. control group, # p,0.05, vs. DIO group. doi:10.1371/journal.pone.0003856.g007 PLoS ONE | www.plosone.org 11 December 2008 | Volume 3 | Issue 12 | e3856