Adaptive Immunity, Autophagy Figure 2. T. gondii-infected CD402/2 mice exhibit upregulation of IFN-c, TNF-a and NOS2 in the eye and brain. B6 and CD402/2 mice were euthanized 4 weeks after infection with tissue cysts of the ME49 strain of T. gondii. A, Levels of IFN-c, TNF-a and NOS2 mRNA in eyes and brains were examined using quantitative PCR and normalized against the levels of 18s rRNA. Each group contained 5 mice. Results are shown as the mean 6 SEM. B, Brains were subjected to immunohistochemistry for NOS2 (X100) and TNF-a (X200). TNF-a was detected not only in the brain parenchyma but also within blood vessel walls in both B6 and CD402/2 mice (inset). C, Brains were lysed and subjected to immunoblot for NOS2, TNF-a and actin. Each lane represents one mouse. Results are representative of 3 to 4 independent experiments. doi:10.1371/journal.pone.0014472.g002 PLoS ONE | www.plosone.org 4 December 2010 | Volume 5 | Issue 12 | e14472