N-Chlorotaurine Inacitvates Shiga Toxin Figure 4. Binding to and penetration of purified Stx2 into human kidney glomerular endothelial cells (GEnC) was inhibited by NCT. (A) FACS analysis of binding of Stx2 to GEnC cells. Purified Stx2 was incubated with 55 mM NCT (1% w/v) in PBS at 37uC for 30 min. Controls with Stx2 PLOS ONE | www.plosone.org 6 November 2012 | Volume 7 | Issue 11 | e47105