Reversal of Lupus Nephritis by SAP Treatment Figure 2. The ratios of SAP to DNA decrease in SLE murine model. 6-week-old female BALB/c mice were immunized subcutaneously with ALD-DNA, UnALD-DNA, or PBS (n = 15) for 3 times in 4 weeks. (A) The dynamics of SAP level in serum of mice immunized with ALD-DNA, UnALD-DNA, or PBS were determined by ELISA assay every 2 weeks. (B) The dynamics of circulating DNA level in serum of mice immunized with ALD-DNA, UnALD- DNA, or PBS were determined using a PicoGreen DNA detection kit (Invitrogen) every 2 weeks. (C) SAP levels in the serum of SLE murine model and controls were tested by ELISA at week 8 after initial immunization. n = 15. (D) Circulating DNA levels in the serum of SLE murine model and controls were determined using a PicoGreen DNA detection kit at week 8 after initial immunization. n = 15. (E) The correlation between SAP and DNA levels in PLoS ONE | www.plosone.org 4 July 2011 | Volume 6 | Issue 7 | e22659