Raf1 Is the DCAF of the CLRC Complex Figure 3. raf1-1 conditionally disrupts heterochromatin. (A) cen1:ade6+ silencing assay in wt, clr4D or raf1-1 cells at 25u, 32u or 36uC. (B) Percentage of lagging chromosomes in anaphase of wt, raf1D and raf1-1 cells at 25uC or 36uC. Cell lacking heterochromatin display higher rates of chromosome missegregation at lower temperature [42]. (C) H3K9me2 ChIP: levels associated with cen-dg relative to act1+ in clr4D and raf1-1 cells normalised to wild-type at 25u or 36uC. Error bars: SD. doi:10.1371/journal.pgen.1002499.g003 PLoS Genetics | www.plosgenetics.org 7 February 2012 | Volume 8 | Issue 2 | e1002499