Aciculatin Induces Apoptosis via p53 Pathway Figure 1. Effect of aciculatin on cell viability and cell cycle in human cancer cells. A, Structure of aciculatin: 8-(2,6-dideoxy-b-ribo- hexopyranosyl)-5-hydroxy- 2-(4-hydroxyphenyl)-7-methoxy-4H-1-benzopyran-4-one sesquihydrate. B, HCT116 cells were incubated with the indicated concentrations of aciculatin (5–10 mM) for 48 h. Cell viability was then determined by MTT assay. C, HCT116 cells were starved overnight, and then incubated with vehicle (0.1% DMSO) or aciculatin (10 mM) for the indicated period. The DNA content was subsequently analyzed by PI staining using PLOS ONE | www.plosone.org 3 August 2012 | Volume 7 | Issue 8 | e42192