cultured for 0, 3 and 24 h without (2) and with (+) 100 U/ml IFNg. Calnexin was assayed in each sample as a loading control. Found at: doi:10.1371/journal.pone.0006787.s005 (0.38 MB TIF) Acknowledgments We thank Dr Marina Freudenberg, Max Planck Institute for Immunobi- ology, Freiburg, and Professor Hans Hengartner, Institute for Experimen- tal Immunology, University of Z u¨rich, for providing interferon receptor knock-out mice and for their hospitality while tissues were collected, to Dr Jens Zerrahn, Max Planck Institute for Infection Biology, Berlin, for the References 1. Boehm U, Klamp T, Groot M, Howard JC (1997) Cellular responses to interferon-c. Annu Rev Immunol 15: 749–795. 2. Martens S, Howard J (2006) The Interferon-Inducible GTPases. Annu Rev Cell Dev Biol 22: 559–589. 3. Taylor GA, Feng CG, Sher A (2007) Control of IFN-gamma-mediated host resistance to intracellular pathogens by immunity-related GTPases (p47 GTPases). Microbes Infect 9: 1644–1651. 4. 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