Exercise and Leptin Action Figure 5. Leptin effects on 12-h cumulative food intake and AMPK/mTOR signaling, in the hypothalami of control and exercised rats. (a) Leptin (1026 M) was administered in control and exercised rats. Animals were immediately exposed to food for a 12-hour period (n = 12–15 animals per group). (b, c, d, e) Representative Western blots of five independent experiments showing hypothalamic lysates from Wistar rats. Phospho-AMPKthr172, threonine-phosphorylated AMPK and total AMPK (b); phospho-ACCser79, serine phosphorylated ACC and total ACC (c); phospho- p70S6Kinasethr389, threonine phosphorylated p70S6Kinase and total p70S6K (d); phospho-4EBP1thr70, threonine phosphorylated 4EBP1 and total 4EBP1 (e). Data are the means6SEM. * p,0.05, ** p,0.01, vs. control group; # p,0.05, ## p,0.01 vs. leptin-stimulated control group. doi:10.1371/journal.pone.0003856.g005 PLoS ONE | www.plosone.org 9 December 2008 | Volume 3 | Issue 12 | e3856