SC Dynamics at Steady State Figure 1. Zip1-GFP incorporates into previously established, full-length SC. Cartoon in (A) outlines the induction experiment designed to explore the dynamics of the full-length SC structure using K39. Most ndt80 mutant meiotic nuclei exhibit full-length SC by 24 hours of sporulation; ndt80 meiotic cells are induced to express ZIP1-GFP at 26 hours of sporulation. Western blot in (B) has been probed with an anti-Zip1 antibody, which detects both untagged and tagged Zip1 species, labeled at right (although relative affinity of this antibody for either version of Zip1 is unknown). (C) Representative images of surface-spread nuclei from uninduced or induced cells. Staining for Zip1 (and/or Zip1-GFP) (red) and Zip1-GFP (green) is shown in top panels while corresponding DAPI-stained DNA (white) is pictured below). Zip1-GFP is barely detectable in most uninduced nuclei but PLOS Genetics | www.plosgenetics.org 4 October 2012 | Volume 8 | Issue 10 | e1002993