DNA Content Analysis and Drug Interactions Figure 2. Representative DNA content histograms. Independent experiments of the indicated samples are shown in each case. Fluorescence is plotted on the x-axis, while the number of cells analyzed is on the y-axis. Reference samples were treated with DMSO, shown at the top. Examples of ‘‘High G1’’ profiles include cells treated with ketoconazole or gemfibrozil, while cells treated with fluoxetine give rise to a ‘‘Low G1’’ DNA content profile. At the bottom, we show a few examples of complex DNA content histograms that were unquantifiable. These include profiles of cells treated with suramin and 5-fluorouracil (antineoplastic agents), and flubendazole (a microtubule blocker used as anti-nematodal). doi:10.1371/journal.pone.0036503.g002 PLoS ONE | www.plosone.org 3 May 2012 | Volume 7 | Issue 5 | e36503