GLS-1 and Germline Development Figure 5. GLS-1 Regulates the Sperm-to-Oocyte Switch. (A–D) Immunofluorescence images of extruded germ lines stained for sperm (SP56, green), oocytes (anti-RME-2, red) and nuclear morphology (DAPI, white). (E) Summary of the genetic cascade regulating the sperm-to-oocyte switch in the hermaphrodite germ line. Previously established genes (in black) taken from [11] and new players are indicated in color. (F) GLS-1(FL) has a stronger affinity for the unique C-terminal half of GLD-3L in GST-pulldown assays. (Top) GLS-1 visualized with anti-His antibodies. (Bottom) Purified full-length GLS-16xHis and GLD-3 fragments fused to GST. Nt-half, covers all KH domains. Ct-half, covers the unique part of GLD-3L. (G) Competition assay for GLD-3L and GLS-1 on bead bound FBF. All proteins are purified from insect cells and carry a C-terminal His tag. FBF is immobilized on beads. GLD-3L was first bound to FBF and subsequently displaced by the addition of increasing amounts of free GLS-1. The decrease of GLD-3L on beads was quantified. doi:10.1371/journal.pgen.1000494.g005 PLoS Genetics | www.plosgenetics.org 9 May 2009 | Volume 5 | Issue 5 | e1000494