p38 MAPK in Epithelial-Mesenchymal Transition Figure 5. Expression of E-cadherin, CK, a-SMA, vimentin in HK-2 cells by Western blot and RT-PCR analysis. (A–B)E-cadherin expression(A1,A2) and a-SMA(B1,B2) were analyzed by Western blot of the HK-2 cells cultured in 5.5 mM glucose (lane 1), 30 mM glucose (lane 2), 30 mM glucose+Cont siRNA(lane 3), 30 mM glucose+p38 siRNA for 24 h(lane 4), 30 mM glucose+p38 siRNA for 48 h(lane 5).(C–D)mRNA of CK (C1,C2) and vimentin(D1,D2) were analyzed by RT-PCR of the HK-2 cells cultured in 5.5 mM glucose (lane 1), 30 mM glucose (lane 2), 30 mM glucose+Cont siRNA(lane 3), 30 mM glucose+p38 siRNA for 24 h(lane 4), 30 mM glucose+p38 siRNA for 48 h(lane 5).Values represent the mean 6 SD, aP,0.05 vs. 5.5 mM glucose, bP,0.05 vs. 30 mM glucose. doi:10.1371/journal.pone.0022806.g005 PLoS ONE | www.plosone.org 5 July 2011 | Volume 6 | Issue 7 | e22806