33. Eicks M, Maurino V, Knappe S, Flu¨ gge UI, Fischer K (2002) The plastidic pentose phosphate translocator represents a link between the cytosolic and plastidic pentose phosphate pathway in plants. Plant Physiol 128: 512–522. 34. Schu¨ nemann D, Borchert S, Flu¨ gge UI, Heldt HW (1993) ATP/ADP translocator from pea root plastids. Comparison with translocators from spinach chloroplasts and pea leaf mitochondria. Plant Physiol 103: 131–137. 35. Reiser J, Linka N, Lemke L, Jeblick W, Neuhaus HE (2004) Molecular physiological analysis of the two plastidic ATP/ADP transporters from Arabidopsis. Plant Physiol 136: 3524–3536. 36. Houston NL, Hajduch M, Thelen JJ (2009) Quantitative proteomics of seed filling in castor: comparison with soybean and rapeseed reveals differences between photosynthetic and nonphotosynthetic seed metabolism. Plant Physiol 151: 857–868. 37. Hajduch M, Matusova R, Houston NL, Thelen JJ (2011) Comparative proteomics of seed maturation in oilseeds reveals differences in intermediary metabolism. Proteomics 11: 1619–1629. 38. Wakao S, Andre C, Benning C (2008) Functional analyses of cytosolic glucose-6- phosphate dehydrogenases and their contribution to seed oil accumulation in Arabidopsis. Plant Physiol 146: 277–288. 39. Hajduch M, Casteel JE, Hurrelmeyer KE, Song Z, Agrawal GK, et al. (2006) Proteomic analysis of seed filling in Brassica napus. Developmental characteriza- tion of metabolic isozymes using high-resolution two-dimensional gel electro- phoresis. Plant Physiol 141: 32–46. 40. Troncoso-Ponce MA, Kilaru A, Cao X, Durrett TP, Fan J, et al. (2011) Comparative deep transcriptional profiling of four developing oilseeds. Plant J 68: 1014–1027. 41. Emes MJ, Bowsher CG, Hedley C, Burrell MM, Scrase-Field ES, et al. (2003) Starch synthesis and carbon partitioning in developing endosperm. J Exp Bot 54: 569–575. 42. Hundertmark M, Hincha DK (2008) LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics 9: 118. 43. Li F, Ma C, Wang X, Gao C, Zhang J, et al. (2011) Characterization of sucrose transporter alleles and their association with seed yield-related traits in Brassica napus L. BMC Plant Biol 11: 168. 44. Wang E, Wang J, Zhu X, Hao W, Wang L, et al. (2008) Control of rice grain- filling and yield by a gene with a potential signature of domestication. Nat Genet 40: 1370–1374. 45. Winter H, Huber SC (2000) Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes. Crit Rev Biochem Mol Biol 35: 253–289. 46. Andriotis VM, Pike MJ, Bunnewell S, Hills MJ, Smith AM (2010) The plastidial glucose-6-phosphate/phosphate antiporter GPT1 is essential for morphogenesis in Arabidopsis embryos. Plant J 64: 128–139. 47. Prabhakar V, Lo¨ ttgert T, Geimer S, Do¨ rmann P, Kru¨ ger S, et al. (2010) Phosphoenolpyruvate provision to plastids is essential for gametophyte and sporophyte development in Arabidopsis thaliana. Plant Cell 22: 2594–2617. 48. Niu Y, Wu GZ, Ye R, Lin WH, Shi QM, et al. (2009) Global analysis of gene expression profiles in Brassica napus developing seeds reveals a conserved lipid metabolism regulation with Arabidopsis thaliana. Mol Plant 2: 1107–1122. 49. Rawsthorne S (2002) Carbon flux and fatty acid synthesis in plants. Prog Lipid Res 41: 182–196. 50. Dennis D, Miernyk J (1982) Compartmentation of non-photosynthetic carbohydrate metabolism. Annu Rev Plant Physiol 33: 27–50. 51. White JA, Todd J, Newman T, Focks N, Girke T, et al. (2000) A new set of Arabidopsis expressed sequence tags from developing seeds. The metabolic pathway from carbohydrate to seed oil. Plant Physiol 124: 1582–1594. 52. Simcox PD, Reid EE, Canvin DT, Dennis D (1977) Enzymes of the glycolytic and pentose phosphate pathways in proplastids from the developing endosperm of Ricinus communis L. Plant Physiol 59: 1128–1132. 53. Kang F, Rawsthorne S (1994) Starch and fatty acid synthesis in plastids from developing embryos of oilseed rape (Brassica napus L.). Plant J 6: 795–805. 54. Alonso AP, Goffman FD, Ohlrogge JB, Shachar-Hill Y (2007) Carbon conversion efficiency and central metabolic fluxes in developing sunflower (Helianthus annuus L.) embryos. Plant J 52: 296–308. 55. Smith RG, Gauthier DA, Dennis DT, Turpin DH (1992) Malate-dependent and pyruvate-dependent fatty-acid synthesis in leucoplastids from developing castor endosperm. Plant Physiol 98: 1233–1238. 56. Sriram G, Fulton DB, Iyer VV, Peterson JM, Zhou R, et al. (2004) Quantification of compartmented metabolic fluxes in developing soybean embryos by employing biosynthetically directed fractional 13C labeling, two- dimensional [13C, 1H] nuclear magnetic resonance, and comprehensive isotopomer balancing. Plant Physiol 136: 3043–3057. 57. Da Silva PMFR, Eastmond PJ, Hill LM, Smith AM, Rawsthorne S (1997) Starch metabolism in developing embryos of oilseed rape. Planta 203: 480–487. 58. Focks N, Benning C (1998) Wrinkled 1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiol 118: 91–101. Gene Expression Profiles in Physic Nut Seeds 59. Periappuram C, Steinhauer L, Barton DL, Taylor DC, Chatson B, et al. (2000) A plastidic phosphoglucomutase from Arabidopsis. A reversible enzyme reaction with an important role in metabolic control. Plant Physiol 122: 1193–1199. 60. Vigeolas H, M o¨hlmann T, Martini N, Neuhaus HE, Geigenberger P (2004) Embryo-specific reduction of ADP–Glc pyrophosphorylase leads to an inhibition of starch synthesis and a delay in oil accumulation in developing seeds of oilseed rape. Plant Physiol 136: 2676–2686. 61. Sarin R, Sharma M, Sinharay S, Malhotra RK (2007) Jatropha–palm biodiesel blends: an optimum mix for Asia. Fuel 86: 1365–1371. 62. Knothe G (2008) ‘‘Designer’’ biodiesel: optimizing fatty ester composition to improve fuel properties. Energy Fuels 22: 1358–1364. 63. Krishnakumar J, Venkatachalapathy KVS, Elancheliyan S (2008) Technical aspects of biodiesel production from vegetable oils. Thermal Science 12: 159–169. 64. Li YH, Beisson F, Pollard M, Ohlrogge J (2006) Oil content of Arabidopsis seeds: The influence of seed anatomy, light and plant-to-plant variation. Phytochemistry 67: 904–915. 65. Kunst L, Taylor DC, Underhill EW (1992) Fatty acid elongation in developing seeds of Arabidopsis thaliana. Plant Physiol Biochem 30: 425–434. 66. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, et al. (1994) Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6: 147–158. 67. Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, et al. (1992) Map- based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258: 1353–1355. 68. Knutzon DS, Bleibaum JL, Nelsen J, Kridl JC, Thompson GA (1992) Isolation and characterization of two safflower oleoyl-acyl carrier protein thioesterase cDNA clones. Plant Physiol 100: 1751–1758. 69. Voelker TA, Jones A, Cranmer AM, Davies HM, Knutzon DS (1997) Broad- range and binary-range acyl-acyl carrier protein thioesterases suggest an alternative mechanism for medium-chain production in seeds. Plant Physiol 114: 669–677. 70. Chen GQ, Turner C, He X, Nguyen T, McKeon TA, et al. (2007) Expression profiles of genes involved in fatty acid and triacylglycerol synthesis in castor bean (Ricinus communis L.). Lipids 42: 263–274. 71. Lu C, Wallis JG, Browse J (2007) An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library. BMC Plant Biol 7: 42. 72. Kim HU, Lee KR, Go YS, Jung JH, Suh MC, et al. (2011) Endoplasmic reticulum-located PDAT1–2 from castor bean enhances hydroxy fatty acid accumulation in transgenic plants. Plant Cell Physiol 52: 983–993. 73. van Erp H, Bates PD, Burgal J, Shockey J, Browse J (2011) Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis. Plant Physiol 155: 683–693. 74. Makkar HPS, Aderibigbe AO, Becker K (1998) Comparative evaluation of non- toxic and toxic varieties of Jatropha curcas for chemical composition, digestibility, protein degradability and toxic factors. Food Chem 62: 207–215. 75. Makkar HPS, Becker K, Schmook B (1998) Edible provenances of Jatropha curcas from Quintana Roo state of Mexico and effect of roasting on antinutrient and toxic factors in seeds. Plant Foods Hum Nutr 52: 31–36. 76. Kwong RW, Bui AQ, Lee H, Kwong LW, Fischer RL, et al. (2003) LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development. Plant Cell 5: 5–18. 77. Kagaya Y, Toyoshima R, Okuda R, Usui H, Yamamoto A, et al. (2005) LEAFY COTYLEDON1 controls seed storage protein genes through its regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3. Plant Cell Physiol 46: 399–406. 78. Bensmihen S, Giraudat J, Parcy F (2005) Characterization of three homologous basic leucine zipper transcription factors (bZIP) of the ABI5 family during Arabidopsis thaliana embryo maturation. J Exp Bot 56: 597–603. 79. Hara A, Radin NS (1978) Lipid extraction of tissues with a low toxicity solvent. Anal Biochem 90: 420–426. 80. Siloto RM, Findlay K, Lopez-Villalobos A, Yeung EC, Nykiforuk CL, et al. (2006) The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis. Plant Cell l18: 1961–1974. 81. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, et al. (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76–85. 82. Fales F (1951) The assimilation and degradation of carbohydrates by yeast cells. J Biol Chem 193: 113–124. 83. Wu PZ, Li J, Wei Q, Zeng L, Chen YP, et al. (2009) Cloning and functional characterization of an acyl-acyl carrier protein thioesterase (JcFATB1) from Jatropha curcas. Tree Physiol 29: 1299–1305. 84. Reich M, Ohm K, Angelo M, Tamayo P, Mesirov JP (2004) GeneCluster 2.0: an advanced toolset for bioarray analysis. Bioinformatics 20: 1797–1798. PLoS ONE | www.plosone.org 12 May 2012 | Volume 7 | Issue 5 | e36522