Regulation of CCL2 Gene Expression Figure 3. p65 overexpression is sufficient for activation of the CCL2 gene in rat islets and b-cell lines. A. 832/13 cells were transduced with increasing concentrations of recombinant adenoviruses expressing either bGAL, FLAG-p65 and FLAG-p65 S276A. Whole cell lysates were harvested 24 h post-transduction and an immunoblot was performed using antibodies against p65 (detecting both endogenous and ectopically PLOS ONE | www.plosone.org 7 October 2012 | Volume 7 | Issue 10 | e46986