Role for PAD2 in Gene Regulation Figure 4. Truncated FLAG-tagged PAD2 proteins reveal regions necessary for nuclear localization. (A) The schematic at the top indicates the different truncated PAD2 proteins of 140, 278 and 437 amino acids. Truncated FLAG-PAD2 constructs were validated by overexpressing in HEK 293 cells with lysates analyzed by SDS-PAGE. Membranes were probed with an anti-FLAG antibody which detected predicted molecular weight of truncated proteins while b-actin shows loading control. (B) Truncation of PAD2 reveals regions of the protein necessary for subcellular PLoS ONE | www.plosone.org 7 July 2012 | Volume 7 | Issue 7 | e41242