Repression of Tumors in miR-143 Transgenic Mice Figure 2. Tumor incidence in ApcMin/+ mice with or without CAG/miR-143 transgene. Tumors of intestines were observed under a stereoscopic microscope. A and B) The number of small intestine tumors whose diameter (W) was more than 1 mm of females (A) and males (B) of W/ APC (W) and Tg/APC (T) is presented. C-E) Incidence of small intestine tumors of W/APC (W) and Tg/APC (T) is presented based on tumor size: (C) 1 mm , the diameter of tumors (W) ,2 mm, (D) 2 mm ,W,3 mm, (E) 3 mm ,W. F and G) The number of colon tumors whose diameter (W) was more than 1 mm of females (F) and males (G) of W/APC (W) and Tg/APC (T) is presented. Tumor incidence is presented by box and whisker plots and median values are given as asterisks. n: numbers of mice examined. doi:10.1371/journal.pone.0042137.g002 Hence, we examined the effect of miR-143 and miR-145 on p68/p72 expression in DLD-1 cells. As shown in Fig. 5B (3rd panel), miR-145 mimic inhibited p72 expression, while neither miR-143 mimic nor siRNA for ERK5 seemed to have significant effect, which data demonstrate that the downregulation of p72 in the small intestine tumors of Tg/APC may be mainly elicited by PLoS ONE | www.plosone.org 4 August 2012 | Volume 7 | Issue 8 | e42137