PARK2 Modifies Synucleinopathy Figure 6. The frequency of PS129-a-synuclein-positive structures immunolabelled with anti-ubiquitin antibodies is lower in the absence than presence of Parkin. (A) Confocal scanning laser micrographs of a brainstem section from a representative symptomatic hA30Pa-syn mouse illustrating colocalization of ubiquitin staining with PS129-a-synuclein-positive deposits (top panel). Note that ubiquitin was present in most of the swollen PS129-a-synuclein-positive neurites (arrows). Synphilin-1-immunoreactivity was not found within the PS129-a-synuclein-positive deposits (middle panel), whereas it was detected in nerve processes in a representative region of the cerebellum (lower panel). gr: granular cell layer; P: Purkinje cell layer; mol: molecular cell layer. Scale bars indicate 25 mm. (B) Percentage of PS129-a-synuclein-immunopositive neurites and cell bodies co-labelled for ubiquitin in a representative region of the brainstem of symptomatic hA30Pa-syn mice with or without Parkin (n = 4–5). *, p,0.05. doi:10.1371/journal.pone.0006629.g006 PLoS ONE | www.plosone.org 7 August 2009 | Volume 4 | Issue 8 | e6629