Found at: doi:10.1371/journal.pone.0011755.s002 (0.06 MB AVI) Video S3 Real-time motion of EGFP-TSHb granules close to the plasma membrane of SIRT1-RNAi pituitary cells was monitored at 5 s intervals by TIRF microscopy. Scale bar, 20 mm. Found at: doi:10.1371/journal.pone.0011755.s003 (0.06 MB AVI) Acknowledgments We thank former and other members of the Setou group for their support and advice, especially Dr Konishi, Dr Tsutsumi and Dr Kurabe for advice; Ms Hatanaka and Dr Sato for their technical assistance; Dr Parlow for References 1. Imai S, Armstrong CM, Kaeberlein M, Guarente L (2000) Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403: 795–800. 2. North BJ, Verdin E (2004) Sirtuins: Sir2-related NAD-dependent protein deacetylases. Genome Biol 5: 224. 3. Oberdoerffer P, Sinclair DA (2007) The role of nuclear architecture in genomic instability and ageing. Nat Rev Mol Cell Biol 8: 692–702. 4. 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