Neuronal COUP-TFII Regulation Figure 3. COUP-TFII mRNA levels in the hypothalamus of STZ treated mice. (A) Glycemia (B) RT-qPCR analysis of mRNA levels. Upper left panel: POMC mRNA levels; upper middle panel: AgRP mRNA levels; upper right panel: NPY mRNA levels; lower left panel: Neuro-D mRNA levels; lower right panel: COUP-TFII mRNA levels. Citrate control mice (%), STZ treated mice (&), STZ mice treated with insulin (grey square). Data are expressed as mean 6 SEM. (n = 6 mice/group). *P#0.05 **P#0.01 compared to citrate treated mice. 1 P#0.05 11P#0.01 compared to STZ treated mice. doi:10.1371/journal.pone.0013464.g003 PLoS ONE | www.plosone.org 5 October 2010 | Volume 5 | Issue 10 | e13464