Table S1 (DOC) Table S2 (DOC) References raf1-R576A and raf1-1 cells. Right: cells grown at 36uC. (C) Westerns of Stc1-FLAG IP analysed for Rik1-Myc in wild-type, raf1-R518A, raf1-R576A and raf1-1 cells. Right: cells grown at 36uC. (D) Northern: centromeric siRNAs in wild-type, raf1-1, clr4D, and raf1D cells. Cells grown at 36uC. Loading control: snoRNA58. (E) RNAPII ChIP in wild-type, clr4D, raf1-R518A and raf1-R576A cells. Diagram (left) indicates position of cen1 primers used (grey bar). RNAPII enrichment (right) was analysed by qPCR relative to tRNA gene primers. Error bar SD. (TIF) List of strains used in this study. List of primers used in this study. 1. Farazi TA, Juranek SA, Tuschl T (2008) The growing catalog of small RNAs and their association with distinct Argonaute/Piwi family members. Develop- ment 135: 1201–1214. 2. Grewal SI (2010) RNAi-dependent formation of heterochromatin and its diverse functions. Curr Opin Genet Dev 20: 134–141. 3. 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PLoS Genetics | www.plosgenetics.org 13 February 2012 | Volume 8 | Issue 2 | e1002499