Exercise and Leptin Action Figure 1. Effects of IL-6 on food intake, hypothalamic AMPK/mTOR activity and ATP content. (a) Effect of i.c.v. administration of IL-6 on food intake; pretreatment with AICAR blocks IL-6-induced anorexia (n = 12–15 animals per group). (b, c, d) Typical chromatographic run (b) depicting the ATP, ADP, and AMP fractions in control (black line) and in i.c.v. IL-6 treated animals (red line), as mean ATP content and as AMP:ATP ratio (c, d). (e, f, g, h) Representative Western blots of four independent experiments showing hypothalamic lysates from Wistar rats. Phospho-AMPKthr172, threonine-phosphorylated AMPK and total AMPK (e); phospho-ACCser79, serine phosphorylated ACC and total ACC (f); phospho-p70S6Kinasethr389, threonine phosphorylated p70S6Kinase and total p70S6K (g); phospho-4EBP1thr70, threonine phosphorylated 4EBP1 and total 4EBP1 (h). Confocal PLoS ONE | www.plosone.org 3 December 2008 | Volume 3 | Issue 12 | e3856