Identification of DAF-12 Target Genes by ChIP-chip Figure 6. daf-12(0) worms are sensitized to changes in miRISC activity. (A) (Left panels) Images of ain-1 RNAi treated rrf-3 or rrf-3; daf- 12(rh61rh411) worms. (Upper right) Photo showing protruding vulva phenotype (arrow). Graph showing effects of ain-1 RNAi treatment on vulva development. (B) Body length was analyzed in rrf-3 or rrf-3; daf-12(rh61rh411) worms treated with ain-2 or nhl-2 RNAi. (C) Effect of control and nhl-2 RNAi on WT or daf-12(rh61rh411) worms carrying SCM::GFP transgene marking seam cells. (D) Quantification of seam cells in worms treated with miRISC RNAi. (E) Effect of ain-1 RNAi on WT or daf-12(rh61rh411) worms carrying an elt-2::GFP transgene marking intestinal nuclei. White bar indicates abnormal extra division. (F) Quantification of intestinal cells in worms treated with miRISC RNAi. Bars denote standard error of mean (SEM). P-values were determined by Student’s t-test. ** p,0.01, *** p,0.001, n.s. (difference is not statistically significant). doi:10.1371/journal.pgen.1002179.g006 PLoS Genetics | www.plosgenetics.org 9 July 2011 | Volume 7 | Issue 7 | e1002179