Diversity in Nuclear Pore Complexes Figure 1. Knockdown of NUP62 or NUP214 in ovarian carcinoma cells (TOV112D) treated with specific siRNAs. A. Constant total protein from siRNA-treated cultures was analyzed for nucleoporin content by immunoblot with the indicated antibodies. Dilutions of the cultures treated with control siRNA are shown for comparison to semi-quantify the extent of knockdown (siRNA knockdown resulted in approximately the same signal intensity as dilution to 25%). B. Immunofluorescence microscopy of TOV112D cells treated with NUP62 or NUP214 siRNAs. Immunofluorescent images represent 3D deconvolved projections of 10–15 um total of optical sections through the z axis. C. Immunofluorescence microscopy of NUP133 and NUP62 or NUP214 in TOV112D cells. Bars represent 2 um. doi:10.1371/journal.pone.0036137.g001 and the central nuclear plane were observed individually. The TOV112D and HEK293 cell lines were chosen for this analysis because they display predominantly disparate or coincident patterns of NUP62 and NUP214 immunolabeling, respectively (discussed below). In HEK293 cells, optical sections through the central plane of the nucleus display overlapping punctate NUP62 and NUP214 signals on the rim of the nucleus, a distribution considered typical for NPC immunolabeling (Fig. 3). The NUP62 and NUP214 also antibodies co-labeled abundant NPCs on the contralateral surfaces of HEK293 nuclei (Fig. 3). Immunolabeling PLoS ONE | www.plosone.org 3 April 2012 | Volume 7 | Issue 4 | e36137