Replication Pausing and Imprinting 32. Eydmann T, Sommariva E, Inagawa T, Mian S, Klar AJ, et al. (2008) Rtf1- mediated eukaryotic site-specific replication termination. Genetics 180: 27–39. 33. Kim SM, Dubey DD, Huberman JA (2003) Early-replicating heterochromatin. Genes Dev 17: 330–335. 34. Vengrova S, Dalgaard JZ (2005) The Schizosaccharomyces pombe imprint—nick or ribonucleotide(s)? Curr Biol : - 15: R326–327; author reply R327. 35. Noguchi E, Noguchi C, McDonald WH, Yates JR, 3rd, Russell P (2004) Swi1 and Swi3 are components of a replication fork protection complex in fission yeast. Mol Cell Biol 24: 8342–8355. 36. Lee JB, Hite RK, Hamdan SM, Xie XS, Richardson CC, et al. (2006) DNA primase acts as a molecular brake in DNA replication. Nature 439: 621–624. 37. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194: 795–823. 38. Brewer BJ, Fangman WL (1987) The localization of replication origins on ARS plasmids in S. cerevisiae. Cell 51: 463–471. 39. Huberman JA, Spotila LD, Nawotka KA, el-Assouli SM, Davis LR (1987) The in vivo replication origin of the yeast 2 microns plasmid. Cell 51: 473–481. 40. Kiger JA, Jr., Sinsheimer RL (1969) Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated-naphthoylated DEAE cellulose. J Mol Biol 40: 467–490. 41. Ruven HJ, Seelen CM, Lohman PH, Mullenders LH, van Zeeland AA (1994) Efficient synthesis of 32P-labeled single-stranded DNA probes using linear PCR; application of the method for analysis of strand-specific DNA repair. Mutat Res 315: 189–195. 42. Schmitt ME, Brown TA, Trumpower BL (1990) A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res 18: 3091–3092. 43. Rosen KM, Lamperti ED, Villa-Komaroff L (1990) Optimizing the northern blot procedure. Biotechniques 8: 398–403. PLoS Genetics | www.plosgenetics.org 18 March 2011 | Volume 7 | Issue 3 | e1001328