TLR and HIF in Human DCs Figure 9. Modulation of proinflammatory cytokine secretion and IL-10 by inhibition of HIF-1a by CTM. (A), (B), (C) and (D), MoDC were stimulated with LPS (1 mg/ml), HA (20 mg/ml) and LTA (5 mg/ml). After 24 hours of incubation, cells were harvested and the supernatants examined for the amounts of IL-6 (A), TNF-a (B), IL-1b (C) and IL-10 (D) with a Luminex multiplex array system. w/o: without stimulus. Mean values 6 SD of 6 experiments with cells from different donors are shown. *p,0.05 vs. mature MoDC (unpaired Student’s t-test). doi:10.1371/journal.pone.0010983.g009 In general, different pharmacokinetic properties (accessibility, excretion, or metabolism) and characters of target molecules (expression, activation, or isoforms) are considered major factors determining drug sensitivity. However, these aspects in relation to CTM, YC-1 and digoxin have not been fully determined to date; thus, the underlying basis for the varying sensitivity and respon- siveness of the maturation process, cytokine production and VEGF secretion to the different inhibitors remains to be investigated. Figure 10. Modulation of proinflammatory cytokine secretion and IL-10 by YC-1. (A), (B), (C) and (D), MoDC were stimulated with LPS (1 mg/ml), HA (20 mg/ml) and LTA (5 mg/ml). After 24 hours of incubation, cells were harvested and the supernatants examined for the amounts of IL- 6 (A), TNF-a (B), IL-1b (C) and IL-10 (D) with a Luminex multiplex array system. w/o: without stimulus. Mean values 6 SD of 4 experiments with cells from different donors are shown. doi:10.1371/journal.pone.0010983.g010 PLoS ONE | www.plosone.org 8 June 2010 | Volume 5 | Issue 6 | e10983