OsLEA3-2 in Salt and Drought Tolerance Response Figure 5. Effect of osmotic or salinity stress on Arabidopsis seedlings from wild type and OsLEA3-2-overexpressing transgenic lines (L35, L48, and L69). (A) Diagram of the T-DNA region of the binary vector pHB::OsLEA3-2; (B) OsLEA3-2 expression level in transgenic Arabidopsis, wild type plants as control; (C, D) Sorbitol or NaCl sensitivity of wild type or transgenic seedlings. Photographs were taken following 9 days of growth on media containing 0 (control), 200 mM sorbitol, or 100 mM NaCl. (E) Fresh/dry weights of 9-day-old seedlings. All samples were measured in triplicate. Statistical significance was determined by Student’s t test. **P,0.01 shown above the bar reprent results significantly different from wild type control. doi:10.1371/journal.pone.0045117.g005 PLOS ONE | www.plosone.org 7 September 2012 | Volume 7 | Issue 9 | e45117