Chondrogenic Potential of CD90+ Progenitors blue (D,I,N,S) and Collagen 2 (E,J,O,T) staining intensity was also decreased in the CD902 negative fraction compared to the CD90+ fraction (Alcian blue: B,G,L,Q, Collagen 2:C,H,M,R) on day 14. Scale bars represent 250 mM. doi:10.1371/journal.pone.0043616.g002 Figure 3. Multi-potent differentiation of CD90+/2 sfMPCs. Normal and OA CD90+ or CD902 sfMPCs were induced to differentiate into osteoblasts or adipocytes and stained with Akaline phosphatase (A) to indentify osteoblasts, or Oil Red-O (B) to identify lipid-containing cells (adipocytes). qRT-PCR was used to determine the relative level of adipocyte specific genes (Adiponectin, PPAR-gamma, [C]) and osteoblastic specific genes (Osteonectin, Sp7/Osterix [D]). * = p.0.05. doi:10.1371/journal.pone.0043616.g003 PLOS ONE | www.plosone.org 5 August 2012 | Volume 7 | Issue 8 | e43616