(TIF) Figure S3 Response of mice to a fasting/re-feeding challenge. A. FFA levels were determined in the serum of 16 week old RC-fed mice at baseline (0), after 24 h fasting (24), and after 3 h of re-feeding (+3). B. Blood glucose levels was measured in whole blood of mice at baseline (0), after 24 h fasting (24), and after 3 h of re-feeding (+3). C. Food intake during the re-feeding phase was determined from the difference in weight of food added at the start and end of the re-feeding phase. D. Insulin levels was determined in plasma of the mice at the indicated times (n = 4–11/ genotype/diet). All values are represented as mean 6 S.E.M. a- p#0.05 HFD-fed control versus HFD-fed mGHRKO, b- p#0.05 RC-fed control versus RC-fed mGHRKO, c- p#0.05 RC-fed control versus HFD-fed control, d- p#0.05 RC-fed mGHRKO versus HFD-fed mGHRKO. Two-way ANOVA. References 1. 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