Cholecystolithiasis Is Associated with Clonorchis Figure 1. C. sinensis eggs in gallbladder stones under a light microscope (original magnification, 6400). a, b, eggs are yellow-brown, and wrapped by bilirubinate granules, and parts of the eggs have lost operculum and have a thick shell; c, d, eggs are wrapped by bilirubinate granules or mucoid matter, with operculum in the front end and miracidium in the egg; e, f, eggs are adhered to or parceled with bilirubinate granules and calcium carbonate crystals, and part of the eggs have lost operculum and have a thick shell. doi:10.1371/journal.pone.0042471.g001 Subjects and Specimens Gallbladder stones from one hundred eighty-three cholecysto- lithiasis patients that had received gallbladder-preserving chole- lithotomies in the Department of General Surgery of The Second People’s Hospital during the period of March 2011 to August 2011 were obtained. All patients were from Guangdong province, and consist of 101 males and 82 females. The mean age was 46.6612.8 (Mean 6SD) years old. The BMI of male and female patients was PLoS ONE | www.plosone.org 2 August 2012 | Volume 7 | Issue 8 | e42471