ETV1 Translocations in Prostate Cancer Figure 1. Full-length ETV1 overexpression in clinical prostate cancers correlates with genomic rearrangement of the complete gene. (A) QPCR on RNA from clinical samples that show ETV1 overexpression. Two primer-pairs were used to determine ETV1 expression: ETV1 exon 1 forward and exon 4 reverse, and exon 11 forward and exon 12 reverse, respectively. Primer sequences are given in Supplementary Table S1. Amplified products were quantified relative to the expression of the porphobilinogen deaminase (PBGD) housekeeping gene. Data were normalized to full length ETV1 overexpressed in the xenograft PC135. A high exon 11/12 to exon 1/4 ratio indicates an ETV1 fusion event, a 1 to 1 ratio indicates overexpression of a full length ETV1 transcript. PC374 is a control xenograft that expresses a TMPRSS2-ETV1 fusion gene. A representative experiment of the six samples that show ETV1 overexpression is depicted. (B) Interphase FISH on fresh-frozen prostate cancer tissue sections. BACs used are indicated below the chromosome 7 region investigated. BAC RP11-124L22 (red) spans ETV1 and RP11-1149J13 (green) overlaps DGKB (left panel). Positions of genes from the top of chromosome 7 are indicated in Mbp. A split signal representing an ETV1 translocation is indicated by an arrow. PLoS ONE | www.plosone.org 3 January 2011 | Volume 6 | Issue 1 | e16332