L-PGDS Compensates for a Lack of PPARc2 Figure 4. Regulation of lipolytic proteins in WAT depots. Western blots from A) epididymal and B) subcutaneous white adipose tissue. All quantifications based on densitometry and normalised to b-actin. Representative blots show N = 3 mice. Quantification N = 6 for each genotype for all genes except subcutaneous ATGL, N = 3. a, P,0.05 WT vs PPARc2, b, P,0.05 WT vs DKO c, P,0.05 PPARc2 vs DKO. doi:10.1371/journal.pone.0039512.g004 Figure 5. Expression of inflammatory markers in WAT depots. Gene expression from epididymal white adipose tissue of; A) markers of different macrophage and T-cell subtypes B) cytokines and chemokines and markers of macrophage polarisation. Gene expression from subcutaneous white adipose tissue of; C) markers of different macrophage and T-cell subtypes D) cytokines, chemokines and markers of macrophage polarisation All mice n = 8 per group C57Bl/6 males 4 months old. a, P,0.05 WT vs PPARc2, b, P,0.05 WT vs DKO c, P,0.05 PPARc2 vs DKO, d P,0.05 L-PGDS vs DKO. doi:10.1371/journal.pone.0039512.g005 PLoS ONE | www.plosone.org 9 July 2012 | Volume 7 | Issue 7 | e39512