Gadd153 Mediates 27-OHC-Induced AD Pathology Figure 5. siRNA to gadd153 reduces alterations in iron homeostasis caused by 27-OHC. Treatment with 27-OHC reduces transferrin receptor (TfR) levels and increases levels of ferritin light chain (FLC) as well as ferritin heavy chain (FHC) (a–d). Staining with Phen-green SK, a probe which the fluorescence of which is quenched by iron, shows an accumulation of iron in slices treated with 27-OHC compared to control slices and treatment with siRNA to gadd153 reduces the intensity of Phen-green SK staining (e). *p,0.05 and **p,0.01 versus controls; #p,0.05 versus 27- OHC. doi:10.1371/journal.pone.0026420.g005 PLoS ONE | www.plosone.org 8 October 2011 | Volume 6 | Issue 10 | e26420