MB Found at: doi:10.1371/journal.pone.0013365.s005 (6.70 MB TIF) Found at: doi:10.1371/journal.pone.0013365.s003 (10.15 TIF) Figure S4 The attenuating effect of cholestyramine resin treatment on fluid accumulation is not due to absorptive removal of norepinephrine. V. parahaemolyticus (107 CFU) were suspend- ed in LB medium with or without 100 mM of norepinephrine (NE) and injected into nontreated or cholestyramine-treated ileal loops. Fluid accumulation in each loop was measured 16 h after infection. Error bars represent SDs for experiments conducted in sextuplicate (n.s., not significant). Found at: doi:10.1371/journal.pone.0013365.s004 (6.52 MB TIF) Figure S5 PCR-based genotyping of non-O1/non-O139 V. cholerae. PCR assays of O1 V. cholerae RIMD2203294 (lane1) and non-O1/non-O139 V. cholerae RIMD2214243 (lane 2) strains were performed to test for the presence of several known virulence genes (vtrA, vtrB, vscN2, vscV2, ctxA, tcpA, hlyA and rtxA). 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