28. Esplugues E, Sancho D, Vega-Ramos J, Martinez C, Syrbe U, et al. (2003) Enhanced antitumor immunity in mice deficient in CD69. J Exp Med 197: 1093–1106. 29. Sancho D, Gomez M, Viedma F, Esplugues E, Gordon-Alonso M, et al. (2003) CD69 downregulates autoimmune reactivity through active transforming growth factor-beta production in collagen-induced arthritis. J Clin Invest 112: 872–882. 30. Martin P, Gomez M, Lamana A, Matesanz Marin AM, Cortes JR, et al. (2010) The leukocyte activation antigen CD69 limits allergic asthma and skin contact hypersensitivity. J Allergy Clin Immunol 126: 355–365, 365 e351–353. 31. Cruz-Adalia A, Jimenez-Borreguero LJ, Ramirez-Huesca M, Chico-Calero I, Barreiro O, et al. (2010) CD69 limits the severity of cardiomyopathy after autoimmune myocarditis. Circulation 122: 1396–1404. 32. Esplugues E, Vega-Ramos J, Cartoixa D, Vazquez BN, Salaet I, et al. (2005) Induction of tumor NK-cell immunity by anti-CD69 antibody therapy. Blood 105: 4399–4406. 33. Sancho D, Gomez M, Martinez Del Hoyo G, Lamana A, Esplugues E, et al. (2006) CD69 targeting differentially affects the course of collagen-induced arthritis. J Leukoc Biol 80: 1233–1241. 34. Alari-Pahissa E, Vega-Ramos J, Zhang JG, Castano AR, Turley SJ, et al. (2012) Differential effect of CD69 targeting on bystander and antigen-specific T cell proliferation. J Leukoc Biol 92: 145–158. 35. Firat H, Garcia-Pons F, Tourdot S, Pascolo S, Scardino A, et al. (1999) H-2 class I knockout, HLA-A2.1-transgenic mice: a versatile animal model for preclinical evaluation of antitumor immunotherapeutic strategies. Eur J Immunol 29: 3112–3121. 36. Vremec D, Shortman K (2008) The isolation and identification of murine dendritic cell populations from lymphoid tissues and their production in culture. Methods Mol Biol 415: 163–178. 37. Lorente E, Infantes S, Barnea E, Beer I, Garcia R, et al. (2012) Multiple viral ligands naturally presented by different class I molecules in transporter antigen processing-deficient vaccinia virus-infected cells. J Virol 86: 527–541. CD69 Role on T Cell Priming 38. Restifo NP, Bacik I, Irvine KR, Yewdell JW, McCabe BJ, et al. (1995) Antigen processing in vivo and the elicitation of primary CTL responses. J Immunol 154: 4414–4422. 39. Lorente E, Garcia R, Mir C, Barriga A, Lemonnier FA, et al. (2012) Role of metalloproteases in vaccinia virus epitope processing for transporter associated with antigen processing (TAP)-independent human leukocyte antigen (HLA)-B7 class I antigen presentation. J Biol Chem 287: 9990–10000. 40. Young LJ, Wilson NS, Schnorrer P, Proietto A, ten Broeke T, et al. (2008) Differential MHC class II synthesis and ubiquitination confers distinct antigen- presenting properties on conventional and plasmacytoid dendritic cells. Nat Immunol 9: 1244–1252. 41. Vremec D, O’Keeffe M, Wilson A, Ferrero I, Koch U, et al. (2010) Factors determining the spontaneous activation of splenic dendritic cells in culture. Innate Immun. 42. Bachmann MF, McKall-Faienza K, Schmits R, Bouchard D, Beach J, et al. (1997) Distinct roles for LFA-1 and CD28 during activation of naive T cells: adhesion versus costimulation. Immunity 7: 549–557. 43. Hara T, Jung LK, Bjorndahl JM, Fu SM (1986) Human T cell activation. III. Rapid induction of a phosphorylated 28 kD/32 kD disulfide-linked early activation antigen (EA 1) by 12-o-tetradecanoyl phorbol-13-acetate, mitogens, and antigens. J Exp Med 164: 1988–2005. 44. Cosulich ME, Rubartelli A, Risso A, Cozzolino F, Bargellesi A (1987) Functional characterization of an antigen involved in an early step of T-cell activation. Proc Natl Acad Sci U S A 84: 4205–4209. 45. Carrero JA, Calderon B, Unanue ER (2006) Lymphocytes are detrimental during the early innate immune response against Listeria monocytogenes. J Exp Med 203: 933–940. 46. Martin P, Gomez M, Lamana A, Cruz-Adalia A, Ramirez-Huesca M, et al. (2010) CD69 association with Jak3/Stat5 proteins regulates Th17 cell differentiation. Mol Cell Biol 30: 4877–4889. PLOS ONE | www.plosone.org 12 October 2012 | Volume 7 | Issue 10 | e48593