Genomics of Rhodococcus for Biofuels Figure 5. Screens of four bacterial species for growth on carbohydrates and alcohols. a) Compounds were clustered according to how R. opacus PD630, R. jostii RHA1, C. glutamicum 13032, and R. eutropha H16 were able to grow on oligosaccharides from 2–4 days. Yellow indicates evidence of growth. b) alcohol and monosaccharide compounds were clustered according to bacterial growth as in a. c) Comparison of three Rhodococcus chromosomes revealed that R. opacus B4 and R. opacus PD630 shared two divergent operons dedicated to galactose and oligogalactoside metabolism but R. jostii RHA1 only had a small piece of this chromosomal region containing the GalK and GalT genes omitting a- and b-galactosidases, Solute Binding Protein (SBP), two Solute Binding Protein Transporter (SBPT) proteins, and a DeoR family transcriptional regulatory protein. doi:10.1371/journal.pgen.1002219.g005 for maltotriose growth that are part of an operon containing OPAG_05551 that breaks down glycogen (glucose a1–4) with Pi to yield glucose-1-P. Glucose 1-P isomerase is also utilized in galactose catabolism to generate the glucose 6-P that is common to glycolysis, pentose phosphate, and Entner Duoderoff catabolic pathways that are all complete pathways in our sequence based metabolic reconstructions of Rhodococcus (http://tinyurl.com/ GLC-6-P). PLoS Genetics | www.plosgenetics.org 9 September 2011 | Volume 7 | Issue 9 | e1002219