Locostatin Impairs Migration Figure 2. DHPE but not locostatin decreases RKIP interactions with Raf-1 and PKC. (A) RKIP association with Raf-1 in the presence or increasing concentrations of locostatin or DHPE. Upper panels: Representative western blots. Lower panels: Bar graphs representative of two independent experiments6range. (B) PKC phosphorylation of RKIP in the presence of increasing concentrations of locostatin or DHPE using RKIP or MBP as substrates. Upper panels: Representative autoradiogram is shown. Lower panels: Bar graphs representative of two independent experiments6range. doi:10.1371/journal.pone.0006028.g002 PLoS ONE | www.plosone.org 4 June 2009 | Volume 4 | Issue 6 | e6028