cleavage at each time point is indicated at the bottom. Positions of the bands corresponding to MAT, HMR, HML, cut MAT, cut HMR, cut HML and matDYZ are indicated. GAL, galactose; h, hours. (TIF) Figure S4 Analysis of repair synthesis products in WT cells, as monitored by PCR. Experiments were performed as in Figure 2B except that in this case only 25 cycles of PCR amplifications were done. (TIF) Figure S5 Genetic analysis of T7/3 translocations in SSE mutants. PFGE followed by Southern analysis using the 3R1 probe of Ura2 5-FOAr survivors in the WT and different mutants combination strains. Black arrows indicate the positions of the T7/ 3-MAT and T7/3-HMR translocations and of the linear chromosome III (Chr. III). (TIF) Figure S6 Genetic analysis of T7/3 translocations in SSE mutants. Details as in Figure S5. (TIF) Figure S7 PFGE analysis of translocations in the HMR-inc WT strain. (A) Schematic representation of chromosomes III. Local- ization of the 3L and 3R1 probes is indicated. PFGE followed by Southern analysis using the 3R1 (B) or 3L probes (C) of Ura2 5- FOAr survivors in the control HMR-inc WT strain. Black arrows indicate the positions of the T7/3-MAT and T7/3-HMR translocations and the circular and linear chromosomes III (Chr. III). (TIF) Figure S8 Genetic analysis of chromosome III circularization in SSE mutants. PFGE followed by Southern analysis using the 3R2 probe of Ura2 5-FOAr survivors in the WT and different mutants combination strains. Black arrows indicate the positions of T7/3- MAT translocations, and the circular and linear chromosomes III References 1. Chen JM, Cooper DN, Ferec C, Kehrer-Sawatzki H, Patrinos GP (2010) Genomic rearrangements in inherited disease and cancer. Semin Cancer Biol 20: 222–233. 2. Agarwal S, Tafel AA, Kanaar R (2006) DNA double-strand break repair and chromosome translocations. DNA Repair (Amst) 5: 1075–1081. 3. Richardson C, Jasin M (2000) Frequent chromosomal translocations induced by DNA double-strand breaks. 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