Generalized DNA Transfer in Y. pseudotuberculosis Table 1. Y. pseudotuberculosis strains and plasmids used for DNA transfer experiments. Strains IP32637 and derivatives IP32637 637-RifR 637-NalR 637DHPI-NalR 637DHPI-RifR 637-irp2K-ureBS 637-irp2K-ureBS-5076T 637-irp2K-ureBS(pGDT4T) 637(pUC4K) 637(pUC4K, pGDT4Dpil) IP32637c 637c-NalR 637c-RifR 637c-irp2K 637c-ureBS IP32953 and derivatives IP32953 953-RifR 953-NalR 953-ureBS(pGDT4) 953-irp2K-ureBS IP32777 and derivatives IP32777 777-RifR 777-irp2K-ureBS E. coli and derivatives v7249 v7249(pSWYps1.1) v4826 v4826::pSWYps1.1 pi3 pi3(R388, pSWYps1.2) v826(R388::pSWYps1.2) Plasmids pUC4K pUC18 pcDNA-2.1 pSW25 pGP704-dfr pKOBEG-sacB pGDT4T pSW23T pSWYps1.1 pSW23 pSWYps1.2 R388 R388::pSWYps1.2 Characteristics Wild type, pYV, pGDT4, serotype I Spontaneous RifR mutant of IP32637 Spontaneous NalR mutant of IP32637 Spontaneously NalR and HPI deleted strain IP32637 Spontaneously RifR and HPI deleted strain IP32637 Insertion of the Kan and Spe cassettes into irp2 and ureB, respectively in 637-RifR 637-irp2K-ureBS tagged with Tmp (5076T), RifR Insertion of the Tmp cassette into pGDT4 of 637-irp2K-ureBS Introduction of pUC4K into 637-RifR Replacement of a portion of the pil locus by a Tmp cassette in strain 637(pUC4K) IP32637 cured of pYV and pGDT4 Spontaneous NalR mutant of IP32637c Spontaneous RifR mutant of IP32637c Insertion of Kan into the irp2 gene of IP32637c-RifR Insertion of Spe into the irp2 gene of IP32637c-RifR Wild type, pYV, pYptb32953, serotype I Spontaneous RifR mutant of IP32953 Spontaneous NalR mutant of IP32953 A 953-NalR transconjugant that has acquired ureBS and pGDT4 from 637-irp2K-ureBS Insertion of the Kan and Spe cassettes into irp2 and ureB, respectively in 953-RifR Wild type, serotype I Spontaneous RifR mutant of IP32777 Insertion of the Kan and Spe cassettes into irp2 and ureB, respectively in 777-RifR Allows pSW23T replication and transfer v249 harboring pSWYps1.1 recA derivative of MG1655 v4826 transconjugant with chromosomal insertion of pSWYps1.1 pir+, R388 pi3 carrying R388 and pSWYps1.2 v826 transconjugant harboring R388::pSWYps1.2 cointegrates High copy number High copy number cloning vector, ColEI oriT, ccdB dfr amplified from pNot::Ptac-dfrB1 Ts, low copy number, sacB, CmR Insertion of a Tmp cassette by allelic exchange with the pGDT4-4kb segment Suicide, mobilizable plasmid, oriVR6K, oriTRP4 Insertion of the region encompassing ISYps1 into pSW23T Suicide, non-mobilizable plasmid, oriVR6K Insertion of ISYps1 from pSW23T into pSW23 Conjugative, IncW plasmid Cointegrate between R388 and pSWYps1.2 Antibiotic resistance None Rif Nal Nal Rif Rif, Kan, Spe Rif, Kan, Spe, Tmp Rif, Kan, Spe, Tmp Rif, Kan Rif, Kan, Tmp None Nal Rif Rif, Kan Rif, Kan, Spe, None Rif Nal Spe, Nal Rif, Kan, Spe None Rif Rif, Kan, Spe Kan, Erm Cm Tc Cm Tmp, Erm Tmp, Cm Tmp, Cm Kan Amp Amp Spe Tmp, Amp Cm Tmp Cm Cm Cm Cm Tmp Tmp, Cm Source or reference Institut Pasteur [3] This study [3] This study This study This study This study This study This study [9] This study This study This study This study Institut Pasteur This study [3] This study This study Institut Pasteur This study This study [57] This study [57] This study [16] This study This study Amersham Amersham Invitrogen [52] [3] [54] This study [16] This study [16] This study F. de La Cruz This study doi:10.1371/journal.pgen.1002529.t001 PLoS Genetics | www.plosgenetics.org 3 March 2012 | Volume 8 | Issue 3 | e1002529