Exercise and Leptin Action Figure 2. Effects of IL-6 on food intake and hypothalamic PI(3)K/mTOR and JAK/STAT activity. (a) Pretreatment with Rapamycin blocks IL-6-induced anorexia (n = 10–12 animals per group). (b, c) Representative Western blots of four independent experiments showing hypothalamic lysates from Wistar rats. Phospho-p70S6Kinasethr389, threonine phosphorylated p70S6Kinase and total p70S6K (b); phospho-4EBP1thr70, threonine phosphorylated 4EBP1 and total 4EBP1 (c). (d) Pretreatment with LY294002 had no effect on anorectic response to IL-6 (n = 10–12). (e) Representative western blot of four independent experiments showing hypothalamic lysates from Wistar rats. Phospho-Aktser473, serine phosphorylated Akt and total Akt. (f) Pretreatment with AG490 blocks IL-6-induced anorexia (n = 10–12 animals per group). (g, h) Representative Western blots of four independent experiments showing hypothalamic lysates from Wistar rats. Phospho-JAK2, tyrosine phosphorylated JAK2 and total JAK2 (g); phospho-STAT3, Tyrosine phosphorylated STAT3 and total STAT3 (h). Data are the means6SEM. **p,0.01, vs. control group; # p,0.05, vs. other groups. doi:10.1371/journal.pone.0003856.g002 PLoS ONE | www.plosone.org 5 December 2008 | Volume 3 | Issue 12 | e3856