TRIF Can Be a Suppressor of TLR Signaling Figure 1. TRIF acts as a negative regulator of rEA-induced MyD88-dependent activation of dendritic cells in vivo. C57BL/6 WT (N = 3– 4), MyD88-KO (N = 3), TRIF-KO (N = 3–4), and MyD88/TRIF-DKO (N = 4) mice were injected with 100 ng of rEA. Splenocytes were harvested at 6 hpi, processed, stained for expression of surface markers, and FACS sorted as described in Materials and Methods. All genotype mock-injected mice (N = 2–3) were included in analysis. One of two representative experiments is shown. Separate sets of WT mice were utilized for comparison with each knockout genotype. Activation of CD11c+, CD192, and CD32 DCs is shown. The bars represent Mean 6 SEM. Statistical analysis was completed using PLoS ONE | www.plosone.org 2 July 2011 | Volume 6 | Issue 7 | e22064