Locostatin Impairs Migration Figure 6. Locostatin impairs cytoskeletal organization independent of RKIP expression. MEFs expressing wild-type, depleted or no RKIP were plated at 26104 cells/coverslip and grown for 48 h before treatment with either DMSO (Control) or locostatin (50 mM) for either 2 or 6 h. Cells were fixed and permeabilized and stained with phalloidin for actin-red. DNA/chromatin was stained with Ho¨ echst 33342-blue. Digital photomicrographs were captured using Openlab Darkroom (Improvision) and a Retiga 1300 color digital camera (Q Imaging). Images from MEFs expressing wild-type RKIP were similar to the RKIP+/+ MEFs which express lentiviral vector control. Intensity of images were adjusted to display qualitative rather than quantitative comparisons so that the intensity of staining in each panel cannot be directly compared. doi:10.1371/journal.pone.0006028.g006 PLoS ONE | www.plosone.org 9 June 2009 | Volume 4 | Issue 6 | e6028