SC Dynamics at Steady State Figure 6. Zip1 subunits do not exit to the same extent that they enter the SC. Cartoon in (A) outlines the induction experiment designed to ask whether Zip1 subunits exit the full-length SC structure. Meiotic nuclei with full length SC built of ZIP1-YFP (green) are induced to express two copies of untagged ZIP1 (SM248) or ZIP1-YFP (SM240), at 22 hours of sporulation. SC intrinsic fluorescence is measured at multiple time points after induction. Scatterplots in (B) depict the fluorescence intensity (expressed in arbitrary units) of segments of SCs comprised predominantly of Zip1-YFP. Uninduced datapoints (orange) show a range of SC intrinsic fluorescence in the absence of induced ZIP1or ZIP1-YFP expression. Induced datapoints (blue) show ranges of SC fluorescence at specific time points after induction of two copies of ZIP1-YFP (left) or untagged ZIP1 (right), starting at PLOS Genetics | www.plosgenetics.org 9 October 2012 | Volume 8 | Issue 10 | e1002993