Telomestatin Uncaps Telomeres Figure 8. Telomestatin induces a delocalization of Topo III from PML and shelterin components but not from BLM. MRC5-V1/YFP- Topo III cells were left untreated or were treated for 48 h with 2 mM telomestatin. A, Quantification of the co-localization between Topo III and PML, TRF2, TRF1, TIN2, and BLM in untreated and treated cells. Results are expressed as the relative percentage of Topo IIIa foci that co-localize with PML, TRF2, TRF1, TIN2, or BLM foci and were determined by analysis of more than 50 nuclei. B, Topo III was detected by fluorescence of YFP-tagged Topo III (green) and BLM was detected by immunofluorescence (red). Dapi staining of DNA is shown in blue. The co-localization of Topo III and BLM was not altered by telomestatin treatment. doi:10.1371/journal.pone.0006919.g008 PLoS ONE | www.plosone.org 9 September 2009 | Volume 4 | Issue 9 | e6919