Ang-1 Reduces Myocardial Infarction in db/db Mice Figure 4. Ang-1 attenuates myocardial/endothelial apoptosis, and reduces cardiac fibrosis and hypertrophy in db/db mouse. A. TUNEL-stained heart sections from sham control db/db mouse, db/db mouse treated with Ad-b-gal or Ad-Ang-1 at 24 hours and 14 days of MI. Myocardial and endothelial apoptotic cells in the infarcted area of the ischemic hearts were identified by TUNEL staining (green) and TUNEL/vWF (red) positive staining, and total nuclei by DAPI counterstaining (blue). B and C. Quantitative analysis of apoptotic cells in sham control db/db mouse and infarcted area of db/db mouse treated with Ad-b-gal or Ad-Ang-1 at 24 hours and 14 days after ischemia. TUNEL or TUNEL/vWF positive cells are expressed as the total number of per mm2. Apoptotic cells were significantly decreased in Ad-Ang-1 compared to Ad-b-gal mice. All data represent mean 6 SD (n = 4 mice); *p,0.05. D. Representative images and quantitative analysis of apoptotic cells in the remote area of infarction of db/db mouse at 24 hours after ischemia. Apoptotic cells were significantly decreased in Ad-Ang-1 treated db/db mouse hearts (n = 5) compared to Ad-b-gal treated db/db mice (n = 7). All data represent mean 6 SD; sham control n = 5, *p,0.05. E. Representative images of TUNEL and SMA-stained heart PLoS ONE | www.plosone.org 6 April 2012 | Volume 7 | Issue 4 | e35905