Automated Cell Cycle Analysis Figure 6. TSA detects changes in interphase and mitotic durations in response to cycloheximide treatment. HeLa H2B-GFP cells were treated with cycloheximide at indicated concentrations. Cells were imaged every 12 min for 48 hours. For each condition, 4 fields in 2 independent wells were imaged and the results for the 8 image series were combined. Image series were analyzed using DCELLIQ (a and b) with the time series approach and half of them were analyzed manually (a). The origin of x-axis in graph (a) was set at 800 minutes as no interphase events had a duration shorter than 800 minutes. doi:10.1371/journal.pone.0025511.g006 PLoS ONE | www.plosone.org 8 September 2011 | Volume 6 | Issue 9 | e25511