SATB1 Regulates Replication Timing Figure 6. Chromatin immunoprecipitation (ChIP) assays of SATB1 binding. A. Locations of primers (a–e) used for ChIP assays. B. ChIP analyses were carried out by using anti-mouse SATB1 antibody (left panel) or purified mouse IgG1 control antibody (central panel) jn HeLaS3 cells stably expressing SATB1. Chromain-immunoprecipitated DNA was purified by MinElute (QIAGEN) and used for quantitative PCR. Error bars represent the mean and standard deviations based on four independent experiments. Relative ratio (SATB1/control) is shown as SATB1-specific binding (right panel). doi:10.1371/journal.pone.0042375.g006 Figure 7. Genome copy number analyses of TRR in synchronized cell population. A. Locations of primers (a, b, c, d and f) used for copy number analyses. B. The procedure for quantification of copy number. C. Time course of DNA replication at various locations in and around TTR. Genome copy numbers of cells synchronously growing from double thymidine block were quantified at various loci at various timepoints. The level of DNA synthesis at 2 hr or 8 hr after release was set as 0% or 100% DNA synthesis, respectively. doi:10.1371/journal.pone.0042375.g007 PLoS ONE | www.plosone.org 10 August 2012 | Volume 7 | Issue 8 | e42375