TGF-b Inhibition in Myeloma Figure 5. Terminally differentiated OBs induce MM cell apoptosis with G1 arrest. A. 5TGM1 cells (56104/mL) were cultured alone or co- cultured with MC3T3-E1 cells with or without mineralized nodules. After culturing for 2 days, 5TGM1 cells were collected, stained with FITC-labeled annexin V and PI, and analyzed by flow cytometry. B. 5TGM1 cells were cultured alone, or cocultured with MC3T3-E1 cells with or without mineralized PLoS ONE | www.plosone.org 8 March 2010 | Volume 5 | Issue 3 | e9870