CRX Regulates RdCVF Figure 7. The proximal CRX binding element controls activity of the mouse and human NXNL1 promoters. (A) Transient transfection analysis performed in Y79 cells with NXNL1 2205/+57 and the 79+134 NXNL1/NXNL2 fusion construct. The 2205/+57 wt and CRX mutant constructs both have a T at position -30 and an A at position -8. Luciferase activity was normalized to control (pRL-TK) and the results displayed as fold induction compared to the pGL4.17 control plasmid. Values represent the average of at least three independent experiments done in triplicate. Error is shown as SEM. ***p,0.001. (B) P0 mouse retinas were electroporated with the RFP rhodopsin expression construct and the indicated GFP construct. Quantified fluorescence intensity of the electroporated retinal explants. Comparison of NXNL1 2205/+57 wild-type (wt) and CRX mutant constructs (mut). The 2205/+57 wt and CRX mutant constructs both have a T at position -30 and an A at position -8. Comparison of Nxnl1 2351/+51 wild-type (wt) and CRX mutant constructs (mut). doi:10.1371/journal.pone.0013075.g007 PLoS ONE | www.plosone.org 11 October 2010 | Volume 5 | Issue 10 | e13075