SOX2 Controls Fate of Brain Tumor Cells Figure 7. Neurosphere culture of CD133+ and CD1332 i-SOX2-DAOY Cells in the absence and presence of Dox. i-SOX2-DAOY cells were FACS sorted into CD1332 and CD133+ populations. SSEA1+ cells were excluded from both populations. (A) RNA was isolated from sorted cells, cDNA was prepared and RT-qPCR was conducted. Values represented are differences in Ct value (normalized by correcting for differences in Ct values of GAPDH), where a positive value indicates increased expression of a given transcript within the CD133+ population. (B) Freshly sorted i-SOX2-DAOY cells were placed into suspension culture in the absence or presence of Dox (1 mg/mL), as described in the Materials and Methods. Photomicrographs were taken after 5 days in culture. doi:10.1371/journal.pone.0044087.g007 PLOS ONE | www.plosone.org 11 August 2012 | Volume 7 | Issue 8 | e44087