41. Boesen HT, Pedersen K, Larsen JL, Koch C, Ellis AE (1999) Vibrio anguillarum resistance to rainbow trout (Onchorynchus mykiss) serum: role of O-antigen structure of lipopolysaccharide. Infect Immun 67: 294–301. 42. Welch TJ, Crosa JH (2005) Novel role of the lipopolysaccharide O1 side chain in ferric siderophore transport and virulence of Vibrio anguillarum. Infect Immun 73: 5864–5872. 43. Aoki T, Nomura J, Crosa JH (1985) Virulence of Vibrio anguillarum with particular emphasis on the outer membrane components. Bull Jap Soc Sci Fish 51: 1249–1254. 44. Suzuki Y, Tasumi S, Tsutsui S, Okamoto M, Suetake H (2003) Molecular diversity of skin mucus lectins in fish. Comp Biochem Physiol 136: 723–730. 45. Klena J, Zhang P, Schwartz O, Hull S, Chen T (2005) The core lipopolysaccharide of Escherichia coli is a ligand for the dendritic-cell-specific intercellular adhesion molecule nonintegrin CD209 receptor. J Bacteriol 187: 1710–1715. 46. Elder K, Vizler C, Kusz E, Karcagi I, Glavinas H, et al. (2009) The role of lipopolysaccharide moieties in macrophage response to Escherichia coli. Biochem Biophys Res Commun 389: 46–51. 47. Murray GL, Attridge SR, Morona R (2006) Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar Typhimurium with macrophages and complement. J Bacteriol 188: 2735–2739. O-Antigen Prevents Phagocytosis by Fish Skin Cells 48. Sald´ıas MS, Ortega X, Valvano MA (2009) Burkholderia cenocepacia O antigen lipopolysaccharide prevents phagocytosis by macrophages and adhesion to epithelial cells. J Med Microbiol 58: 1542–1548. 49. Zhang P, Snyder S, Feng P, Azadi P, Zhang S, et al. (2006) Role of N- acetylglucosamine within core lipopolysaccharide of several species of gram- negative bacteria in targeting the DC-SIGN (CD209). J Immunol 177: 4002–4011. 50. Zelensky AN, Gready JE (2004) C-type lectin-like domains in Fugu rubripes. BMC Genomics 5: 51. 51. Milton DL, O’Toole R, Ho¨rstedt P, Wolf-Watz H (1996) Flagellin A is essential for the virulence of Vibrio anguillarum. J Bacteriol 178: 1310–1319. 52. McGee K, Ho¨rstedt P, Milton DL (1996) Identification and characterization of additional flagellin genes from Vibrio anguillarum. J Bacteriol 178: 5188–5198. 53. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory. 54. Preston MA, Penner JL (1987) Structural and antigenic properties of lipopolysaccharides from serotype reference strains of Campylobacter jejuni. Infect Immun 55: 1806–1812. 55. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685. 56. Plante CJ (2000) Role of bacterial exopolymeric capsules in protection from deposit-feeder digestion. Aquat Microb Ecol 21: 211–219. PLoS ONE | www.plosone.org 13 May 2012 | Volume 7 | Issue 5 | e37678