incubated in DMEM 2/2/2(100 ml, 100 nM) with Attractene (1.5 ml) at 25uC for 20 min. The transfection mix was added to the cells dropwise. Cells were collected at 72 hrs post transfection, lysed, and extracts (50 mg) were loaded onto a 4–20% SDS-PAGE gel. Western blots were performed using antibodies against p65, p300, IKK-b, and b-Actin. Densitometry was performed using ImageJ software (Wayne Rasband, NIH, USA). Acknowledgments Rachel Van Duyne is a predoctoral student in the Microbiology and Immunology Program of the Institute for Biomedical Sciences at the George Washington University. This work is from a dissertation to be References 1. Easley R, Van Duyne R, Coley W, Guendel I, Dadgar S, et al. (2010) Chromatin dynamics associated with HIV-1 Tat-activated transcription. Biochim Biophys Acta 1799: 275–285. 2. Gallo RC (2005) History of the discoveries of the first human retroviruses: HTLV-1 and HTLV-2. Oncogene 24: 5926–5930. 3. 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Blood 104: 1490–1497. 44. Giebler HA, Loring JE, van Orden K, Colgin MA, Garrus JE, et al. (1997) Anchoring of CREB binding protein to the human T-cell leukemia virus type 1 PLoS ONE | www.plosone.org 13 July 2012 | Volume 7 | Issue 7 | e40662