HK Inhibits Angiogenesis/Peritoneal Dissemination Figure 8. Effects of honokiol on the phosphorylation of STAT-3 in gastric cancer cells, HUVECs, and tumors. (A) The p-STAT-3 (Tyr705) expressions in gastric cancer cells (AGS) and HUVECs treated with honokiol (HK, 10 and 20 mM) for 1 h were detected by a confocal microscope. (B) Tumors in nude mice were established for 7 days after the intraperitoneal inoculation of MKN45 cells. Mice were then injected intraperitoneally with honokiol (5 mg/kg/twice per week) for 28 days. The p-STAT-3 (Tyr705) expressions in metastatic tumors isolated from mice with or without HK treatment are shown. The p-STAT-3 (Tyr705) protein expressions, stained dark brown, were detected by immunohistochemistry. All data are presented as mean 6 SEM of five independent experiments. *p,0.05 as compared with control. doi:10.1371/journal.pone.0043711.g008 PLOS ONE | www.plosone.org 9 August 2012 | Volume 7 | Issue 8 | e43711