Subcellular Dynamics in Plants Figure 9. ocFLM of BRI1-GFP and aquaporin-GFP reveals gradients in their subcellular environment. (A) GFP fluorescence lifetimes (filled squares) and the corresponding intensity profiles (open circles) recorded over 5.0 mm plasmalemma-cell wall sections of two different hypocotyl cells from two independent BRI1-GFP expressing Arabidopsis seedlings. (B) GFP fluorescence lifetimes (filled squares) and the corresponding intensity profiles (open circles) recorded over 4.0 mm plasmalemma-cell wall sections of two different hypocotyl cells from two independent aquaporin-GFP expressing Arabidopsis seedlings. (C) GFP fluorescence lifetime (filled squares) and the corresponding intensity profile (open circles) over a 6.0 mm plasmalemma-cell wall area of a hypocotyl cell from a BRI1-GFP expressing Arabidopsis seedling. The white line in the confocal image inlet shows the recorded section. For the calculation of the fluorescence lifetime values and error bars see Material and Methods. Additional BRI1-GFP and aquaporin-GFP fluorescence lifetime measurements are presented in Fig. 10D and E. doi:10.1371/journal.pone.0005716.g009 PLoS ONE | www.plosone.org 10 May 2009 | Volume 4 | Issue 5 | e5716