Novel Promoters for Human NAIP Figure 2. Identification of novel NAIP transcription start sites. A) Diagram of transcription start sites identified in the NAIPfull (top) and NAIP1/2 (bottom) copies by 59 RACE. In the center, shaded block arrows indicate polarity of genes encoded on 5q13.2 (as in Figure 1a) and enlargements of NAIPfull and NAIP1/2 are shown above and beneath this representation. Their orientation is shown opposite to which they are encoded and black boxes represent exons. Checkered and striped block arrows indicate localization and orientation of Alus and the previously identified NAIP LTR promoters [15], respectively. Not all repeat elements are shown. Black double arrowheads represent primers used in nested RT-PCR to uncover NAIP TSS in this and a previous analysis [15], represented by stick diagrams in top- and bottom-most images. All sequenced clones arising from Alus, and PLoS ONE | www.plosone.org 4 June 2009 | Volume 4 | Issue 6 | e5761