Gaucher Disease Therapy Figure 4. Analyses of GC and GS levels in the cortex and liver by LC/MS. (A) Cortex GC and GS levels in IFG treated 4L;C* did not show significant reduction relative to the untreated mice. GC and GS levels remained accumulated at terminal stage in 600 mg/kg/d IFG treated 4L;C* brain. (B) Increased liver GC were detected in 600 mg/kg/d IFG treated 4L;C* mice at 14 and 44 days relative to untreated 4L;C* liver. GS level decreased in 20 mg/kg/d IFG treated 4L;C* liver at 28 days. GS level in 600 mg/kg/d IFG treated 28 day liver were higher than untreated 4L;C* liver. The data represent the mean6S.E. and analyzed by Student’s t-test. *, p,0.05 (n = 3 mice). The GC and GS levels were normalized by protein level in the tissues lysate. doi:10.1371/journal.pone.0019037.g004 PLoS ONE | www.plosone.org 5 April 2011 | Volume 6 | Issue 4 | e19037