Sb Modulates avb5 Integrin Activity expressed b5 integrin subunit. Moreover, upon SbS treatment, b5 integrin was re-distributed at the basal side of the epithelial intestinal cells. This suggests that avb5integrin participates in SbS- mediated epithelial cell migration. However, it should be noted that SbS increased the b5 subunit integrin expression level of E- cadherin negative cells located into the lamina propria, suggesting that SbS could also act on non-epithelial cells, as recently described [31]. Figure 3. SbS down-modulates cell adhesion to vitronectin. (A) Isolated HCT-8/E11 cells were treated with or without SbS (dilution 1/8) and plated on either fibronectin or vitronectin at the indicated concentrations. Cell-ECM adhesion was evaluated as described in Methods. Results are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. (B) HCT-8/E11 cells were treated with or without dilutions of SbS and plated on vitronectin (Vn). Cell-vitronectin adhesion was evaluated as described in Methods. (C) HCT-8/E11 cells were treated with or without SbS, seeded in vitronectin-coated or fibronectin-coated wells and allowed to adhere for 2 h at 37uC. Spreading cells were counted microscopically. Results are expressed as the percentage of spreading cells. Data represent the mean+SD of 3 separate experiments. *** P,0.001. doi:10.1371/journal.pone.0045047.g003 PLOS ONE | www.plosone.org 5 September 2012 | Volume 7 | Issue 9 | e45047