Rapid Mutation by OPEN ZFNs Table 1. Recognition helix (RH) amino acid sequences and B2H activities of zinc finger arrays for endogenous zebrafish gene targets. ZFN Name Site Name F1 subsite/RH sequence F2 subsite/RH sequence F3 subsite/RH sequence Mean B2H fold-activation S.D. of B2H fold-activation kdr ZFP1 kdr ZFP2 OZ453 OZ454 OZ455 OZ456 OZ457 OZ458 OZ459 OZ460 OZ461 OZ462 OZ463 OZ464 OZ465 OZ466 OZ467 OZ468 OZ469 OZ470 OZ471 OZ472 OZ473 OZ474 OZ475 OZ476 OZ477 OZ478 OZ479 OZ480 OZ481 OZ482 OZ483 OZ484 OZ485 OZ486 OZ487 OZ488 kdr_2L kdr_2R DT_16L DT_16L DT_16L DT_16L DT_16L DT_16L DT_16R DT_16R DT_16R DT_16R DT_16R HIF_12L HIF_12L HIF_12L HIF_12L HIF_12L HIF_12R HIF_12R HIF_12R HIF_12R HIF_12R Telo_16L Telo_16L Telo_16L Telo_16R Telo_16R Telo_16R Telo_16R Telo_16R Telo_16R TfR2_2L TfR2_2L TfR2_2L TfR2_2L TfR2_2L TfR2_2L GTGt RSDALTR GGAc QKGHLTR GTCc TSTLLNR TMAVLRR TSTLLKR TRADLRR TSTLLNR TGVLLRR GTCa TRAVLAR TRAVLRR TAAVLTR TRAVLAR TGAVLTR GGGt KAERLRR RNTHLAR KKDHLHR KGERLVR KGERLVR GGTg IPNHLAR IPNHLAR TKQKLQV QKHHLAV IPNHLAR GGAg DKTKLRV DKTKLRV DKTKLSV GCTg QRQALDR QRQALDR SAQALAR QRQALDR STQALRR SRQALGR GCTc TRPMLRR LSQTLKR THSMLAR MNSTLIR MKNTLTR TTQALRR GCTg GGT LRHHLTR GTG RSDALTR GTG RKQVLTN RREVLEN RGEVLIN RTEVLTN RGEVLAN RREVLMN GAC DAGNLTR DGSNLAR DRANLTR DAGNLTR DKGNLKR TAG RSDNLKS RSDNLTT RADNLQT RMDNLST RMDNLST GGA QSAHLKR QKPHLTN QNPHLTN QSAHLKR QRPHLTN GAT VRHNLTR VKHNLHR VAHNLTR GAA QTGNLLR QGSNLQR QGGNLTR QTGNLQR QATNLQR QSANLSR GGG RGEHLTR RREHLMR RREHLVR RVDHLHR RQEHLVR RREHLMR GAA GAA QKANLTR TTG RSDSLLG GAA QEVNLNR QTVNLDR QTVNLDR QNTNLSR QSVNLRR QDGNLGR GGG RIDKLGG RIDKLGG RIDKLGD RIDKLGG RNDKLVT GTG RGDALAR RGDALAR RKRNLTG RKDALNR RKDALNR GCA QDVSLVR QATTLRR QSNVLSR QDVSLVR QTATLKR GTA QSTSLQR QSGTLTR QGTSLAR GAA QRNNLGR QRNNLGR QHPNLTR QHPNLTR QHPNLTR QHPNLTR GGA QGGHLKR QNSHLRR QTTHLRR QNSHLRR QKPHLSR QTTHLSR GAT 7.19 1.14 3.06 7.27 2.97 4.35 4.76 1.94 3.79 3.80 4.18 2.94 3.47 4.90 8.43 4.95 7.39 6.22 7.22 5.80 0.92 3.22 3.67 9.74 7.78 6.29 7.48 5.79 9.43 7.72 5.93 5.79 4.93 32.92 5.58 7.45 7.19 6.62 0.70 0.40 0.16 1.55 0.51 1.45 0.13 0.28 0.55 0.02 0.05 0.05 0.48 0.48 0.56 0.70 0.51 0.94 0.57 0.08 0.06 0.12 0.21 0.37 0.86 0.46 0.46 0.17 0.20 0.67 0.68 0.36 0.08 8.91 0.98 1.34 0.14 0.46 PLoS ONE | www.plosone.org 4 February 2009 | Volume 4 | Issue 2 | e4348