In Vitro Model of the Glomerular Capillary Wall Figure 9. Immunofluorescence demonstrating appearance of GEnC (A–C) and podocyte (D–F) cell layers after one week at 336C and one week at 376C on the collagen/PCL/mesh bioartificial composite membrane. PECAM-1 on GEnC labelled red and podocin on podocytes green. (A and B) GEnC cultured in a single layer or (C) with podocytes co-cultured on the opposite side of the membrane. (D and E) podocytes cultured in a single layer or (C) with GEnC co-cultured on the opposite side of the membrane. Scale bars 250 mm (A and D) and 50 mm (B, C, E and F). doi:10.1371/journal.pone.0020802.g009 Figure 10. Scanning EM demonstrating morphology of GEnC (A and B) and podocytes (C and D) co-cultured on opposite sides of the collagen/PCL/mesh bioartificial composite membrane. Scale bars 100 mm (A and C) and 20 mm (B and D). GEnC form a uniform monolayer, comparable with those grown in monoculture or co-culture with podocytes on porous supports in tissue culture inserts (Fig. 2), whilst podocytes are less densely packed. doi:10.1371/journal.pone.0020802.g010 PLoS ONE | www.plosone.org 8 June 2011 | Volume 6 | Issue 6 | e20802