RNAi in the Eusocial Wasp Polistes directs hexamerin uptake in the rice moth Corcyra cephalonica. Insect Biochemistry and Molecular Biology 38: 307–319. 46. Zhou X, Oi FM, Scharf ME (2006) Social exploitation of hexamerin: RNAi reveals a major caste-regulatory factor in termites. Proceedings of the National Academy of Sciences USA 103: 4499–4504. 47. Tomoyasu Y, Denell RE (2004) Larval RNAi in Tribolium (Coleoptera) for analyzing adult development. Development Genes and Evolution 214: 575–578. 48. Mutti NS, Wang Y, Kaftanoglu O, Amdam GV (2011) Honey bee PTEN - description, developmental knockdown, and tissue-specific expression of splice- variants correlated with alternative social phenotypes. PLoS ONE 6: e22195. 49. Hunt JH, Buck NA, Wheeler DE (2003) Storage proteins in vespid wasps: characterization, developmental pattern, and occurrence in adults. Journal of Insect Physiology 49: 785–794. 50. Nasrin N, Kaushik VK, Fortier E, Wall D, Pearson KJ, et al. (2009) JNK1 phosphorylates SIRT1 and promotes its enzymatic activity. PLoS One: e8414. 51. Havukainen H, Halskau Ø, Skjarven L, Smedal B, Amdam GV (2011) Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N- terminus. Journal of Experimental Biology 214: 582–592. 52. Henshaw M, Toth A, Young T (2011) Development of new microsatellite loci for the genus Polistes from publicly available expressed sequence tag sequences. Insectes Sociaux, (In press). 53. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 22DDCT method. Methods 25: 402–408. 54. Kucharski R, Maleszka R (2005) Microarray and real-time PCR analyses of gene expression in the honeybee brain following caffein treatment. Journal of Molecular Neuroscience 27: 269–276. 55. Rossi AM, Hunt JH (1988) Honey supplementation and its developmental consequences: evidence for food limitation in a paper wasp, Polistes metricus. Ecological Entomology 13: 437–442. 56. Hines HM, Hunt JH, O’Connor TK, Gillespie JJ, Cameron SA (2007) Multigene phylogeny reveals eusociality evolved twice in vespid wasps. Proceedings of the National Academy of Sciences USA 104: 3295–3299. 57. Karsai I, Hunt JH (2002) Food quantity affects traits of offspring in the paper wasp Polistes metricus (Hymenoptera: Vespidae). Environmental Entomology 31: 99–106. 58. Naumann ID (1991) Hymenoptera. The Insects of Australia. Ithaca: Cornell University Press. pp 916–1000. PLoS ONE | www.plosone.org 12 November 2011 | Volume 6 | Issue 11 | e26641