Lung Cell Mechanoresponse to Force Directionality Figure 4. Role of cytoskeleton integrity, and Ca2+ and tyrosine phosphorylation-dependent signaling in lung cell mechan- oresponses. (A–C) Effect of inhibitors against actin (latA) or microtubule (nocodazole) polymerization on (A) EC and ET, (B) EC/ET and (C) F* on CCD- 19Lu fibroblasts probed with RGD-coated FE-AFM tips. (D-F) Effect of the tyrosine phosphatases inhibitor (PAO), and the extracellular (EGTA) and intracellular (BAPTA/AM) Ca2+ chelators on (D) EC and ET, (E) EC/ET and (F) F* probed in the same conditions as in (A–C). The legend of the bar filling patterns shown at the top of (A) and (D) apply to plots (A–C) and (D–F), respectively. Statistical analysis was performed as in Figure 3. doi:10.1371/journal.pone.0032261.g004 PLoS ONE | www.plosone.org 7 February 2012 | Volume 7 | Issue 2 | e32261