Subcellular Localization of Class II HDAs Figure 4. Particle bombardment in onion epidermal tissues. A. Onion tissues were transfected with HDA-GFP plasmids to visualize the pertinent localization of Class II HDAs. HDA5 and HDA8 both localize in the nucleus and cytoplasma while HDA14 cytoplasmic. Still, HDA15 remained nuclear. B. Onion tissues transfected with HDA5-GFP shows its localization in the cytoskeletal network with the dynamic movement of HDA5 spots (arrows) along these web-like structures. Pictures were taken at 2 sec intervals with the same onion cell. doi:10.1371/journal.pone.0030846.g004 PLoS ONE | www.plosone.org 6 February 2012 | Volume 7 | Issue 2 | e30846