cel-miR-268 cel-miR-269 cel-miR-273 dme-miR-100^ cel-miR-357 dme-miR-277^ cel-miR-785 cel-miR-86^ dme-miR-987^ cel-miR-787 cel-miR-231^ dme-miR-993^ cel-miR-790 dme-miR-263b^ D. melanogaster H. sapiens dme-miR-31a^ dme-miR-31b^ dme-miR-31a^ dme-miR-31b^ hsa-miR-99b^ hsa-miR-100^ hsa-miR-31^ hsa-miR-873^ hsa-miR-31^70 hsa-miR-99a^ hsa-miR-99b^ hsa-miR-100^ hsa-miR-302a^ hsa-miR-302b^ hsa-miR-302c^ hsa-miR-302d^ hsa-miR-545*(^) hsa-miR-559^ hsa-miR-99a*(^) hsa-miR-99b*(^) hsa-miR-556-5p^ hsa-miR-96^ hsa-miR-183^ hsa-miR-200a^ hsa-miR-514^ hsa-miR-96^ hsa-miR-182^ hsa-miR-183^ hsa-miR-200a^ hsa-miR-514^ hsa-let-7a^ hsa-let-7b^ hsa-let-7c^ hsa-let-7e^ hsa-let-7f^ hsa-let-7g^70 hsa-let-7i^ hsa-miR-98^ hsa-miR-202^ hsa-let-7a^ MicroRNAs of Worms and Flies Table 6. cont. Table 6. cont. miRNA probe Conserved miRNA Sequences miRNA probe Conserved miRNA Sequences cel-miR-791 dme-miR-263b^ dme-bantam^ dme-miR-306*(^) hsa-miR-450b-3p^ C. elegans cel-miR-54^ cel-miR-56^ cel-miR-72^ cel-miR-73^70 cel-miR-74^ cel-miR-266^ cel-miR-269^ cel-miR-72^ cel-miR-73^ cel-miR-74^ cel-miR-266^70 cel-miR-268^ cel-miR-51^ cel-miR-52^ cel-miR-53^ cel-miR-54^ cel-miR-55^ cel-miR-56^70 cel-miR-232^ cel-miR-63^ cel-miR-64^ cel-miR-65^ cel-miR-66^ cel-miR-228^ cel-miR-229^ cel-miR-791^ cel-miR-63^ cel-miR-64^ cel-miR-65^ cel-miR-66^ cel-miR-228^ cel-miR-229^ cel-miR-790^ cel-let-7^ cel-miR-48^ cel-miR-84^ cel-miR-241^ cel-miR-794^ cel-miR-795^ D. melanogaster H. sapiens dme-miR-963^ dme-miR-977^70 dme-miR-984^ dme-let-7^ dme-miR-963^ dme-miR-977^ dme-miR-984^ dme-miR-1^ dme-miR-2a^ dme-miR-2b^ dme-miR-2c^ dme-miR-6^ dme-miR-11^ dme-miR-13a^ dme-miR-13b^ dme-miR-308^ dme-bantam^ hsa-miR-98^ hsa-miR-196a^ hsa-let-7b^ hsa-let-7c^ hsa-let-7d^ hsa-let-7e^ hsa-let-7f^ hsa-let-7g^ hsa-let-7i^ hsa-let-7a^ hsa-let-7b^ hsa-let-7c^ hsa-let-7d^ hsa-let-7e^ hsa-let-7f^ hsa-let-7g^ hsa-let-7i^ hsa-miR-98^ hsa-miR-1^ hsa-miR-122^ hsa-miR-206^ hsa-miR-499-3p^ hsa-miR-450b-3p^ cel-miR-795 cel-miR-796 cel-miR-797 cel-miR-1018 cel-miR-1022 C. elegans cel-miR-48^ cel-miR-84^ cel-miR-241^ cel-miR-793^ cel-miR-795^ cel-let-7^ cel-miR-48^ cel-miR-84^ cel-miR-241^ cel-miR-793^ cel-miR-794^ cel-miR-1^ cel-miR-256^ cel-miR-2^ cel-miR-43^ cel-miR-250^ cel-miR-58^ cel-miR-80^ cel-miR-81^ cel-miR-82^ cel-miR-1022^ cel-miR-58^ cel-miR-80^ cel-miR-81^ cel-miR-82^ cel-miR-1018^ cel-miR-793 dme-let-7^ dme-miR-977^ dme-miR-984^ cel-miR-794 cel-let-7^ dme-let-7^ PLoS ONE | www.plosone.org 73 C. elegans miRNAs have significant identity at their 59 ends and/or $70% similarity over their entire sequences to both fly and human miRNAs. All the 73 C. elegans miRNAs have 59 related sequences in both flies and humans, whereas 16/73 C. elegans miRNAs are also classified as $70% homologous over length to miRNAs in flies and humans. For detail in sequence relationships refer to Figure S1. ^indicates miRNAs with 59 end sequence homology present in worms, flies and humans. Superscript 70 denotes miRNAs with $70% similarity over full sequence across the three analyzed species. It should be noted that the miRNA registry was extensively modified in the year 2007 (releases 10.0 and 10.1), introducing changes to previous mature miRNA sequences as well as adding new mature miRNA sequences to C. elegans (5), D. melanogaster (75) and human (494) miRNA databases. We performed our analysis using the latest miRBase release (10.1). We elected to use C. elegans sequences as reference anchors because of the general availability of deletions for mir genes. doi:10.1371/journal.pone.0002818.t006 16 July 2008 | Volume 3 | Issue 7 | e2818