PINK1 and Drp1 Figure 3. PINK1 protects against rotenone-induced loss of mitochondrial connectivity. (A) Living cells were imaged after transfection with mito-YFP. Cells expressing PINK1 had longer mitochondria compared to control lines (kinase dead shown here). Rotenone induced fragmentation in control lines but not in lines expressing PINK1. Scale bar in the lower right panel is 2 mm, applies to all fluorescence micrographs. (B) FRAP curves demonstrate that control cells showed more recovery under control conditions (open circles) than after 24 h exposure to 100 nM rotenone (closed circles). In this experiment, control cells are parental M17 cells that were transfected only with the mito-YFP vector to image mitochondria (labeled vector). (C) In contrast, cells stably transduced with wild-type PINK1 showed very similar FRAP curves whether this was measured in the presence of PLoS ONE | www.plosone.org 7 May 2009 | Volume 4 | Issue 5 | e5701