EcNAC1 Confers Abiotic Stress-Tolerance in Tobacco Figure 1. Bioinformatic analysis of NAC protein sequences. (A) Phylogram was derived for rice, sorghum and maize NAC protein sequences which clustered with EcNAC1 using the program ClustalW. (B) Prediction of putative motifs shared among these protein sequences using MEME program. Numbered boxes represent different putative motifs (annotations of these motifs in EcNAC1 are listed in Table S3). doi:10.1371/journal.pone.0040397.g001 Figure 2. Quantitative expression of EcNAC1 gene in response to water-deficit and salt stress. (A) Adapting a gradual stress imposition protocol, 25-day-old finger millet seedlings were subjected to water-deficit stress following gravimetric approach. (B) NaCl treatment was given to seedlings 3-days after germination. Accumulation of EcNAC1 transcripts was determined by qRT-PCR with 2 mg of total RNA. The Actin gene was used as normalizer. doi:10.1371/journal.pone.0040397.g002 PLoS ONE | www.plosone.org 5 July 2012 | Volume 7 | Issue 7 | e40397