Viral Vectored Blood Stage Malaria Vaccine Figure 7. Comparison of antibody and growth inhibitory activity of rabbit IgG after vaccination with mono-allelic or bi-allelic PfAMA1 vaccines. New Zealand rabbits (n = 4) were primed with 161010 vp 1) ChAd63-biallelic, 2) AdHu5-biallelic or 3) AdHu5-PfAMA1 (3D7). Groups 1) and 2) were boosted with 16107 pfu of the bi-allelic MVA construct and group 3) was boosted with the mono-allelic MVA-PfAMA1 (3D7). The AMA1 specific antibody response against both homologous 3D7 (A) and FVO (B) PfAMA1 recombinant protein was measured in the serum two weeks after the final immunization (day 70). There was no significant difference in the antibody responses between the groups against the 3D7 or FVO alleles. The % GIA values for individual rabbits and the median are shown against both the 3D7 (C) and FVO (D) strains at 5 mg/ml IgG concentration. There was a significant positive correlation between the 3D7 and FVO GIA (R2 = 0.56, *P #0.05) (E). doi:10.1371/journal.pone.0020977.g007 PLoS ONE | www.plosone.org 9 June 2011 | Volume 6 | Issue 6 | e20977