KAR, Stress & Steroids Figure 2. ROD of GluR6 mRNA in the dentate gyrus (A) CA3 (B) and CA1 (C) after adrenalectomy and treatment with vehicle (ADX), aldosterone (ADX+Aldo) RU28,362 (ADX+RU362) or corticosterone (ADX+CORT). (D) A representative autoradiogram of GluR6 mRNA. *-significantly different from sham and ADX+CORT (p,0.05, n = 8). doi:10.1371/journal.pone.0004328.g002 or 400 mg/ml corticosterone. As shown in Figure 6, treatment with a moderate dose of 25 mg/ml, but not a high dose of 400 mg/ ml of corticosterone significantly (p,0.05) increased KA1 mRNA levels in the dentate gyrus (F (2,16) = 6.504) but did not reach significance in the CA3. Apoptosis in the dentate gyrus Adrenalectomy produced a 46% (p,0.00001) increase (from 3.2 to 4.8% of total cell profiles) in the number of pyknotic cells in the dentate gyrus relative to sham adrenalectomized animals (data not shown). Figure 3. ROD of GluR7 mRNA in the dentate gyrus (A) after adrenalectomy and treatment with vehicle (ADX), aldosterone (ADX+Aldo) RU28,362 (ADX+RU362) or corticosterone (ADX+CORT). (B) A representative autoradiogram of GluR6 mRNA. *-significantly different from sham and ADX+CORT (p,0.05, n = 8). doi:10.1371/journal.pone.0004328.g003 PLoS ONE | www.plosone.org 3 January 2009 | Volume 4 | Issue 1 | e4328