TRIM50 Promotes Aggresome Formation and Clearance Figure 3. p62 deletion mutants interacting with TRIM50. (A) Schematic representation of p62 deletion mutants with the minimal region of interaction with TRIM50. Lysates from FLAG-TRIM50#3 expressing a number of p62 deletion mutants were immunoprecipitated with anti-FLAG and immunoblotted with anti-GFP (B) and anti-GST (C). doi:10.1371/journal.pone.0040440.g003 PLoS ONE | www.plosone.org 6 July 2012 | Volume 7 | Issue 7 | e40440