Signature in Renal Carcinoma Figure 3. Loss of developmental transcription factors in clear cell renal cell carcinoma. a. A microarray heatmap showing significant downregulation of four developmental transcriptional factors in ccRCC and a table showing fold changes of DTFs and their known renal developmental function. b. IHC validation of decreased expression of TFAP2B, DMRT2, and TFCP2L1. Sum scores are shown with n, as indicated. *p,0.01 when comparing ccRCC to normal match. c. Microarray heatmap showing downregulation of TFCP2L1 and its regulated genes in ccRCC. S– Stage, N–normal, T–tumor. Upregulation of genes is indicated in red, downregulation is indicated in green, and similar expression is indicated in yellow, as generated by Genetree. doi:10.1371/journal.pone.0010696.g003 PLoS ONE | www.plosone.org 6 May 2010 | Volume 5 | Issue 5 | e10696