PWM-Induced B Cell Activation Figure 3. Synergistic effects of TLR ligands with poke weed lectin. A: Effect of TLR2 blockage on human B cell proliferation in response to PWM. CD19+ human peripheral blood B cells were labelled with CFSE, preincubated with anti-TLR2 mAb or the corresponding isotype control (murine IgG1k), and stimulated with Pam3CSK4 or PWM (10 mg/ml) 6 SpA (5 mg/ml) for 5 days. Proliferation was assessed by CFSE dilution. The gate denotes the percentage of live gated proliferating B cells as depicted. One representative experiment of n = 3 is shown. B: Synergistic effects of PWM and immunostimulatory DNA. Human B cells were stained with CFSE for assessment of B cell proliferation. Stimulation was performed with CpG ODN 2006 (PTO), DNAse- or mock (reaction buffer only)-treated PWM, 2006 GC (PTO) or CpG 2006 (PO) and combinations thereof as indicated. The graphs show the results from one representative experiment from n$3. The percentage of proliferating B cells is indicated in the graphs. doi:10.1371/journal.pone.0029806.g003 PLoS ONE | www.plosone.org 7 January 2012 | Volume 7 | Issue 1 | e29806