VIG and Vig2 Impact Chromatin cytoplasmic material of Drosophila S2 cells. We found both proteins in nuclear and in cytoplasmic extracts (Figure 2). These results suggest that VIG and Vig2 function both in the nucleus and in the cytoplasm. The broad peak of VIG and of Vig2 in the nuclear and cytoplasmic fractions suggests that in each compartment these proteins are likely to be associated with other factors in complexes of different sizes and possibly of different functions. Figure 2. Recovery of VIG and Vig2 from nuclear and cytoplasmic S2 cell extracts. (A) Nuclear and cytoplasmic material was subjected to Superose-6 column chromatography. The numbers above the Western image represent different fractions. Peaks of size standards used to calibrate the column are shown. The Western blot was performed using an antiserum that recognizes both VIG1 and Vig2 (CSH1803). (B) Segregation of nuclear and cytoplasmic components in the extracts used for column chromatography is verified by a control Western blot for HP1 (nuclear) and Dynein (cytoplasmic). doi:10.1371/journal.pone.0006182.g002 PLoS ONE | www.plosone.org 3 July 2009 | Volume 4 | Issue 7 | e6182