Context-Specific Notch Targets Figure 9. RBP-J binding site sequences of potential direct Notch1 target genes. Regulatory regions comprising 2000 bp before to 500 bp after the transcription start site of the Notch1 target genes Hes1, Hes5, Sox9, Pax6, Id4, Hey1, Runx1, and Myf5 were screened for potential RBP-J binding sites (red arrow head indicates direction of binding) using the ‘Transcription Element Search System’ (TESS; http://www.cbil.upenn.edu/tess; [67]) and a weight matrix from Ong et al. [68]. As a threshold, a Lg-likelyhood score (La) of 10 had to be exceeded. Binding sites were projected onto respective genomic regions using the UCSC Genome Browser (http://genome.ucsc.edu/) using the Feb. 2006 (NCBI36/mm8) assembly. doi:10.1371/journal.pone.0011481.g009 PLoS ONE | www.plosone.org 13 July 2010 | Volume 5 | Issue 7 | e11481