Vps45 Regulates Tlg2 and Snc Levels Figure 4. Vps45L117R complements growth phenotypes of vps45D cells. (A) The specific growth rate (m) and doubling time of wild-type (SF838-9D) and congenic vps45D mutant (NOzY1) cells producing either no HA-Vps45 (carrying the vector YEplac195), wild-type HA-Vps45 (VPS45) or HA-Vps45L117R (L117R) from pCOG070 and pCOG071 respectively (WT and vps45D cells producing no HA-Vps45 carried the vector YEplac195) was determined during logarithmic growth in minimal media. Data represent the mean 6 SEM of 6 independent sets of cells. 1, P,0.05 versus WT; #, P,0.05 versus vps45D. (B) Wild-type cells (SF838-9D) harbouring YEPlac195 or pCOG070 (HA-Vps45), and congenic vps45D mutant cells (NOzY1) harbouring plasmids YEplac195 (empty vector), pCOG070 (HA-Vps45), pCOG71 (HA-Vps45L117R) or pCOG072 (HA-Vps45W244R) were analysed as described for Figure 3B. (C) All yeast strains in this panel harbour plasmid pCOG054, producing HA-Snc2 in addition to the following plasmids. Wild- type cells (SF838-9D) harbouring YCplac111 (empty vector) and congenic vps45D mutant cells (NOzY1) harbouring YCplac111 (empty vector) or pNB706 (HA-Vps45), pNB707 (HA-Vps45L117R) or pNB708 (HA-Vps45W244R) were analyzed as in Figure 3B. doi:10.1371/journal.pone.0049628.g004 PLOS ONE | www.plosone.org 5 November 2012 | Volume 7 | Issue 11 | e49628