PI3K & Rbsn-5 in Drosophila Figure 4. Fmi, DE-cadherin and Arm show intracellular accumulation in PI3K (III)-knockdown pupal wings. (A) Confocal immunodetection showing XY and XZ sections in the plane of pupal wings at 30–32hr APF. Flamingo [Fmi], DE-cadherin [DE-cad] and Armadillo [Arm] (red) were localized to the ZA in the plasma membrane in wild type, but accumulated to the intracellular puncta in dVps15-knockdown cells. The regions where the GFP-2xFYVE and/or dsRNA for dVps15 were expressed by the dpp-Gal4 driver are indicated by double-headed arrows. (B) Fmi mostly accumulated to GFP-LAMP1 positive compartments in dVps15-knockdown pupal wing cells at 30 hr APF while DE-cadherin co-localized only partly. Arrows indicate GFP-LAMP1 positive compartments that harbor Fmi or DE-cadherin. Fmi and DE-cadherin location showed little overlap. The scale bars represent 10 mm. doi:10.1371/journal.pone.0007306.g004 PLoS ONE | www.plosone.org 5 October 2009 | Volume 4 | Issue 10 | e7306