Sequence (59-39)a CCGCGGCGCCATTGACCAATTTCAG GGATCCGCAACTCTCGTAATGTAAAC AAGTCGTGCTGCTTCATGTG CGGTGATATTCTCATTTTAGCC CGAATCGAATTCAGGAAC GGGAAGTTCTATGCTTATGG GCTCGCTTCTTTAAACTCAACC TCTAGAAAGGCTCACTCTACCCTATT CTCTTGGCATTCTTTACACCACACCCCGGGAGGCTCGAGGCTGATATCTTCCTTATCCG CGGATAAGGAAGATATCAGCCTCGAGCCTCCCGGGGTGTGGTGTAAAGAATGCCAAGAG CGCAGCGATAAAGGCGTGGTG GTCGACAAGACTTTCACCTGGAAACGC GCTTTATCGCCCACTTTCAAG CCACAATAGCCGTAACGCTTACCCGGGAGGCTCGAGCATGTTTTCTCCTTGTGATTAG CTAATCACAAGGAGAAAACATGCTCGAGCCTCCCGGGTAAGCGTTACGGCTATTGTGG GGCATGGAATTGTCAATCC GATAGCCTTATTGTAATC GATTACAATAAGGCTATC CATATGAAAAGATTAGAAACTTTGGAATCCATTTT CTCGAGTTATTAACATTCACTCTCTTGG GAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATGTGTAGGCTGGAGCTGCTTC TCATGTCTACGCCGTATTAATAGATAATGCCAATCACCATATGAATATCCTCCTTAGTTC GGTTTGCCTGGGTTGGAT GATTTTGCGGTATTTTG GCGGCTGAAGAATACGAG CTGATCAGCCAAATACAA AAGCCCTGTAGCGTTTGATT CCCAATTCCAACCAGAGCCA GAGCGCTTGAGGATGTCTATC GTGATCATGGTGTTCTTTAGC CTACAAAATTTGCATAACG CCACAAAATTTGCATAAAG GCAACTCTCGTAATGTAAAC CCAAAGAGGGTAAAGAGAGCG CCTCTCCATCGCTTCTCTAAC Table 2. Primers used in this study. Primerb sodB promoter primers sodB-F1 (SacII) sodB-R1 (BamHI) Screening and Sequencing primers gfp-1 aphA3-2 sacBSCN-F2 HpKanSacSCN-R HpsodBSCN-R Cloning primers FurCF (XbaI) HpUKanSacR (XhoI, SmaI) HpDKanSacF (XhoI, SmaI) HpDKanSacR FurCR (SalI) USod-F USod-R DSod-F DSod-R SodBMt-R SodBMt-F HP_Fur_expression F2 (NdeI) HP_Fur_expression R2 (Xho1) Red_EC_Fur_F Red_EC_Fur_R RPA primers amiE-RPA-F amiE-RPA-R pfr-RPA-F pfr-RPA-R sodB-RPA-F sodB-RPA-R fur RPA F fur RPA R EMSA primers G27 sodB EMSA-F 26695 sodB EMSA-F sodB EMSA-R rpoB EMSA-F rpoB EMSA-R aRestriction endonuclease sites are underlined, and linker bases are in bold type. bImportant restriction sites are included in parentheses. doi:10.1371/journal.pone.0005369.t002 Biolabs) and BamHI (Invitrogen). The resulting promoter fragment was then ligated into the appropriately digested pTM117 vector to create pDSM236. The fusion was confirmed by PCR amplification with sodB-F1 and gfp-1 [18] primers and by sequencing with the aphA3-2 primer [18]. pDSM236 was naturally transformed into WT G27 and DSM300, and transfor- mants were selected on HBA plates containing 25 mg/ml Kan and 25 mg/ml Kan plus 8 mg/ml Cm, respectively. The WT strain bearing pDSM236 was designated DSM238, and DSM300 bearing pDSM236 was designated DSM308. GFP reporter assays The ability of the sodB transcriptional fusion to drive the expression of GFP was assessed using flow cytometry as described PLoS ONE | www.plosone.org 3 April 2009 | Volume 4 | Issue 4 | e5369 sodB in H. pylori Reference This study This study [18] [18] This study This study This study [18] This study This study This study [18] This study This study This study This study This study This study This study This study This study This study [7] [7] [18] [18] This study This study [18] [18] This study This study This study This study This study