late phase of the anagen stage. (B and C) Higher magnification views of the boxed regions in A. B, epidermal region; C, hair follicle region. CnAß was observed in both epidermal and follicular keratinocytes. Scale bar, 100 mm. (TIF) Figure S5 Distribution of CnAß-FK mRNA and NFATc2 in rat skin at postnatal days 24 and 25 (early phase of the anagen stage). (A–D) Distribution of CnAß-FK mRNA. (A and B), postnatal day 24 (P24); (C and D), postnatal day 25 (P25). (B and D) Sections were counter-stained with hematoxylin (blue). (E and F) Immunohistochemical analysis of NFATc2 in hair follicles at P24 and P25. In HG cells, NFATc2 was present in the cytoplasm. DP, dermal papillae; HG, hair germ; SG, sebaceous gland. Scale bars, 50 mm. (TIF) Figure S6 Distribution of CnAß-FK mRNA in human skin. CnAß-FK is also expressed in human hair follicle. (TIF) Figure S7 Effect of CnAß-FK overexpression on cyclin G2 expression in Hela cells. HeLa cells were transfected with the CnAß-FK/pcDNA3.1-V5-His vector. The cells were harvest- ed 6, 12 and 24 h after the transfection and the amount of cyclin G2 at each time point was measured by Western blotting. V5 is an epitope tag fused with CnAß-FK. (TIF) Figure S8 Cyclosporin A reduces cyclin G2 expression in hair follicles through NFATc2 inhibition. (A) Time-scale of the hair cycle in male Wister rats. The intensity of gray shading indicates the rate of proliferation of follicular keratinocytes as described in Fig. 3a. (B–E) NFATc2 and cyclin G2 localization in anagen stage hair follicles of a control rat. Arrows indicate nuclear References 1. Paus R, Cotsarelis G (1999) The biology of hair follicles. N Engl J Med 341: 491–497. 2. Fuchs E (2007) Scratching the surface of skin development. Nature 445: 834–842. 3. Jiang H, Yamamoto S, Kato R (1995) Induction of anagen in telogen mouse skin by topical application of FK506, a potent immunosuppressant. 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