Identification of RKIP Ligands Figure 6. Increasing doses of compound 26 does not change EGF-induced MAPK activity. HeLa cells expressing wild-type (A) or depleted RKIP (B) were serum starved overnight and then pre-treated for 30 min with increasing doses of DMSO (control) or Compound 26. EGF (10 ng/ml) was added in the final 5 minutes of incubation. Whole cell lysates (30 mg) were separated on SDS-PAGE gels (12.5%), transferred to nitrocellulose and PLoS ONE | www.plosone.org 9 May 2010 | Volume 5 | Issue 5 | e10479