infection with 0.1 ml of bacteria (16106 UFC/mouse) in pyrogen- free PBS (pH 7.2). Fibrin D-dimers were assessed by the semiquantitative D-Di testH latex agglutination assay (Diagnostica Stago). The levels of IL-6, IL-10, IL-12p40 and TNF-a were estimated by ELISA (eBioscience) in the sera of mice (n = 5) infected intraperitoneally with 106 CFU of BABDolsB or BAB- parental. For leukocyte counts, mice were intraperitoneally infected with 16106 CFU of BABDolsB or BAB-parental or 105 CFU of S. typhimurium SL1344 in pyrogen-free sterile PBS (pH 7.2), or with pyrogen-free sterile PBS as a control. Blood was collected from the retrorbital plexus at different time points in tubes with 2mg/ml of EDTA. Then, 5 ml of ice cold PBS were injected in the peritoneal cavity, and 3.0 to 4.5 ml of fluids were collected with a syringe. After centrifugation, the peritoneal cells were resuspended in 0.2 ml of PBS, and total leukocytes, neutrophils, monocytes and lymphocytes were counted. 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Rojas-Jimenez K, Sohlenkamp C, Geiger O, Martinez-Romero E, Werner D, et al. (2005) A ClC chloride channel homolog and ornithine-containing PLoS ONE | www.plosone.org 10 January 2011 | Volume 6 | Issue 1 | e16030