Movie S5 Rotation of immunofluorescence imaging of P. berghei ookinete, labelled with Pbs28 (Red), rabbit anti-Act239–253 (Green) and DAPI (Blue) highlighting labelling at nuclear periphery and pellicle. See Figure 3. (MOV) Movie S6 Rotation of 3D-SIM imaging of P. falciparum merozoite invading, labelled with RON4 (Green), rabbit anti- Act239–253 (Red) and DAPI (Blue). See Figure 6. (MOV) Acknowledgments We thank the Australian Red Cross for supplying human red blood cells, the Walter & Eliza Hall Institute Monoclonal Facility for antibody References 1. WHO (2010) World Health Report. Geneva: World Health Organization. 2. Cowman AF, Crabb BS (2006) Invasion of red blood cells by malaria parasites. Cell 124: 755–766. 3. Prudencio M, Rodriguez A, Mota MM (2006) The silent path to thousands of merozoites: the Plasmodium liver stage. Nat Rev Microbiol 4: 849–856. 4. Vlachou D, Schlegelmilch T, Runn E, Mendes A, Kafatos FC (2006) The developmental migration of Plasmodium in mosquitoes. 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