25. Obaldia N, 3rd, Milhous W, Kyle D (2009) Adaptation of a Thai multidrug- resistant C2A clone of Plasmodium falciparum to Aotus monkeys and its preliminary in vivo antimalarial drug efficacy-resistance profile. Am J Trop Med Hyg 81: 587–594. 26. Silamut K, White NJ (1993) Relation of the stage of parasite development in the peripheral blood to prognosis in severe falciparum malaria. Trans R Soc Trop Med Hyg 87: 436–443. 27. Pukrittayakamee S, Clemens R, Pramoolsinsap C, Karges HE, Vanijanonta S, et al. (1992) Polymorphonuclear leucocyte elastase in Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg 86: 598–601. 28. Gomez CR, Nomellini V, Faunce DE, Kovacs EJ (2008) Innate immunity and aging. Exp Gerontol 43: 718–728. 29. Nomellini V, Gomez CR, Kovacs EJ (2008) Aging and impairment of innate immunity. Contrib Microbiol 15: 188–205. 30. Chelvarajan RL, Collins SM, Van Willigen JM, Bondada S (2005) The unresponsiveness of aged mice to polysaccharide antigens is a result of a defect in macrophage function. J Leukoc Biol 77: 503–512. 31. Chelvarajan RL, Liu Y, Popa D, Getchell ML, Getchell TV, et al. (2006) Molecular basis of age-associated cytokine dysregulation in LPS-stimulated macrophages. J Leukoc Biol 79: 1314–1327. 32. Boehmer ED, Goral J, Faunce DE, Kovacs EJ (2004) Age-dependent decrease in Toll-like receptor 4-mediated proinflammatory cytokine production and mitogen-activated protein kinase expression. J Leukoc Biol 75: 342–349. 33. Plackett TP, Boehmer ED, Faunce DE, Kovacs EJ (2004) Aging and innate immune cells. J Leukoc Biol 76: 291–299. 34. Inamizu T, Chang MP, Makinodan T (1985) Influence of age on the production and regulation of interleukin-1 in mice. Immunology 55: 447–455. 35. Hasko G, Nemeth ZH, Vizi ES, Salzman AL, Szabo C (1998) An agonist of adenosine A3 receptors decreases interleukin-12 and interferon-gamma production and prevents lethality in endotoxemic mice. Eur J Pharmacol 358: 261–268. 36. Liaudet L, Mabley JG, Soriano FG, Pacher P, Marton A, et al. (2001) Inosine reduces systemic inflammation and improves survival in septic shock induced by cecal ligation and puncture. Am J Respir Crit Care Med 164: 1213–1220. 37. Liaudet L, Mabley JG, Pacher P, Virag L, Soriano FG, et al. (2002) Inosine exerts a broad range of antiinflammatory effects in a murine model of acute lung injury. Ann Surg 235: 568–578. 38. Schrier DJ, Lesch ME, Wright CD, Gilbertsen RB (1990) The antiinflammatory effects of adenosine receptor agonists on the carrageenan-induced pleural inflammatory response in rats. J Immunol 145: 1874–1879. Improved P. falciparum Mouse Model 39. Mabley JG, Pacher P, Liaudet L, Soriano FG, Hasko G, et al. (2003) Inosine reduces inflammation and improves survival in a murine model of colitis. Am J Physiol Gastrointest Liver Physiol 284: G138–144. 40. Marton A, Pacher P, Murthy KG, Nemeth ZH, Hasko G, et al. (2001) Anti- inflammatory effects of inosine in human monocytes, neutrophils and epithelial cells in vitro. Int J Mol Med 8: 617–621. 41. Barankiewicz J, Cohen A (1985) Purine nucleotide metabolism in resident and activated rat macrophages in vitro. Eur J Immunol 15: 627–631. 42. Hasko G, Kuhel DG, Nemeth ZH, Mabley JG, Stachlewitz RF, et al. (2000) Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock. J Immunol 164: 1013–1019. 43. Fibach E, Rachmilewitz E (2008) The role of oxidative stress in hemolytic anemia. Curr Mol Med 8: 609–619. 44. Marsh K, Forster D, Waruiru C, Mwangi I, Winstanley M, et al. (1995) Indicators of life-threatening malaria in African children. N Engl J Med 332: 1399–1404. 45. Ishikawa F, Yasukawa M, Lyons B, Yoshida S, Miyamoto T, et al. (2005) Development of functional human blood and immune systems in NOD/SCID/ IL2 receptor {gamma} chain(null) mice. Blood 106: 1565–1573. 46. Serbina NV, Hohl TM, Cherny M, Pamer EG (2009) Selective expansion of the monocytic lineage directed by bacterial infection. J Immunol 183: 1900–1910. 47. Engel DR, Maurer J, Tittel AP, Weisheit C, Cavlar T, et al. (2008) CCR2 mediates homeostatic and inflammatory release of Gr1(high) monocytes from the bone marrow, but is dispensable for bladder infiltration in bacterial urinary tract infection. J Immunol 181: 5579–5586. 48. Sponaas AM, Freitas do Rosario AP, Voisine C, Mastelic B, Thompson J, et al. (2009) Migrating monocytes recruited to the spleen play an important role in control of blood stage malaria. Blood 114: 5522–5531. 49. Waymack JP, Gallon L, Barcelli U, Trocki O, Alexander JW (1987) Effect of blood transfusions on immune function. III. Alterations in macrophage arachidonic acid metabolism. Arch Surg 122: 56–60. 50. Van Rooijen N (1989) The liposome-mediated macrophage ’suicide’ technique. J Immunol Methods 124: 1–6. 51. van Rooijen N (1992) Liposome-mediated elimination of macrophages. Res Immunol 143: 215–219. 52. Giarratana MC, Kobari L, Lapillonne H, Chalmers D, Kiger L, et al. (2005) Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells. Nat Biotechnol 23: 69–74. 53. Galinski MR, Barnwell JW (2008) Plasmodium vivax: who cares? Malar J 7 Suppl 1: S9. PLoS ONE | www.plosone.org 12 March 2011 | Volume 6 | Issue 3 | e18045