30. Smith MA, Rodriguez JJ, Whitfield JB, Deans AR, Janzen DH, et al. (2008) Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proc Natl Acad Sci USA 105: 12359–12364. 31. Rosenberg (2007) Statistical tests for taxonomic distinctiveness from observations of monophyly. Evolution 61: 317–323. 32. Coleman AW (2009) Is there a molecular key to the level of "biological species" in eukaryotes? A DNA guide. Molecular Phylogenetics and Evolution 50: 197–203. 33. Muller T, Philippi N, Dandekar T, Schultz J, Wolf M (2007) Distinguishing species. Rna-a Publication of the Rna Society 13: 1469–1472. 34. Frankham R (2010) Where are we in conservation genetics and where do we need to go? Conservation Genetics 11: 661–663. 35. Mayr E (1942) Systematics and the origin of species, from the viewpoint of a zoologist. Cambridge: Harvard University Press. 36. Bensasson D, Zhang D, Hartl DL, Hewitt GM (2001) Mitochondrial pseudogenes: evolution’s misplaced witnesses. Trends Ecol Evol 16: 314–321. 37. Floyd R, Abebe E, Papert A, Blaxter M (2002) Molecular barcodes for soil nematode identification. Molecular Ecology 11: 839–850. 38. Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biological Conservation 61: 1–10. 39. Cohen JE, Jonsson T, Carpenter SR (2003) Ecological community description using the food web, species abundance, and body size. Proceedings of the National Academy of Sciences 100: 1781–1786. 40. Cohen JE (1978) Food Webs and Niche Space. Princeton: Princeton University Press. 41. Emmerson MC, Raffaelli D (2004) Predator-Prey Body Size, Interaction Strength and the Stability of a Real Food Web. Journal of Animal Ecology 73: 399–409. 42. Brown JH, Gillooly JF (2003) Ecological food webs: High-quality data facilitate theoretical unification. Proceedings of the National Academy of Sciences 100: 1467–1468. 43. Acs Z, Challis RJ, Bihari P, Blaxter M, Hayward A, et al. (2010) Phylogeny and DNA barcoding of inquiline oak gallwasps (Hymenoptera: Cynipidae) of the Western Palaearctic. Molecular Phylogenetics and Evolution 55: 210–225. 44. Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biological Conservation 61: 1–10. 45. Cavender-Bares J, Kozak KH, Fine PVA, Kembel SW (2009) The merging of community ecology and phylogenetic biology. Ecology Letters 12: 693–715. 46. Smith MA, Fisher B (2009) Invasions, DNA barcodes, and rapid biodiversity assessment using ants of Mauritius. Frontiers in Zoology 6: 31. 47. Zhou X, Adamowicz S, Jacobus L, DeWalt RE, Hebert P (2009) Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada. Frontiers in Zoology 6: 30. 48. Crawford AJ, Lips KR, Bermingham E (2010) Epidemic disease decimates amphibian abundance, species diversity, and evolutionary history in the highlands of central Panama. Proceedings of the National Academy of Sciences 107: 13777–13782. 49. Smith MA, Fernandez Triana J, Roughley R, Hebert PDN (2009) DNA Barcode Accumulation Curves for understudied taxa and areas. Molecular Ecology Resources 9s1: 208–216. 50. Schultz J, Wolf M (2009) ITS2 sequence-structure analysis in phylogenetics: A how-to manual for molecular systematics. Molecular Phylogenetics and Evolution 52: 520–523. 51. Ruhl MW, Wolf M, Jenkins TM (2009) Compensatory base changes illuminate morphologically difficult taxonomy. Molecular Phylogenetics and Evolution 54: 664–669. 52. Avise JC (2009) Phylogeography: retrospect and prospect. Journal of Biogeography 36: 3–15. Cryptic Species in a Food Web 53. Higgs PG (2000) RNA secondary structure: physical and computational aspects. Quarterly Reviews of Biophysics 33: 199–253. 54. van Veen FJF, Belshaw R, Godfray HCJ (2003) The value of the ITS2 region for the identification of species boundaries between Alloxysta hyperparasitoids (Hymenoptera: Charipidae) of aphids. European Journal of Entomology 100: 449–453. 55. Birky Jr. CW, Adams J, Gemmell M, Perry J (2010) Using Population Genetic Theory and DNA Sequences for Species Detection and Identification in Asexual Organisms. PLoS ONE 5: e10609. doi:10610.11371/journal.pone.0010609. 56. Wiley EO (1978) The Evolutionary Species Concept Reconsidered. Systematic Biology 27: 17–26. 57. de Queiroz K (2007) Species Concepts and Species Delimitation. Systematic Biology 56: 879–886. 58. Cracraft J (1989) Speciation and its ontology: the empirical consequences of alternative species concepts for understanding patterns and processes of differentiation. In: Otte D, Endler J, eds. Speciation and its consequences. SunderlandMa.: Sinauer Assoc. pp 28–59. 59. Virgilio M, Backeljau T, Nevado B, De Meyer M (2010) Comparative performances of DNA barcoding across insect orders. BMC Bioinformatics 11: 206. 60. O’Meara BC (2010) New Heuristic Methods for Joint Species Delimitation and Species Tree Inference. Systematic Biology 59: 59–73. 61. Austerlitz F, David O, Schaeffer B, Bleakley K, Olteanu M, et al. (2009) DNA barcode analysis: a comparison of phylogenetic and statistical classification methods. BMC Bioinformatics 10: S10. 62. Zhang AB, Heb LJ, Crozier RH, Muster C, Zhu C-D (2010) Estimating sample sizes for DNA barcoding. Molecular Phylogenetics and Evolution 54: 1035–1039. 63. Rougerie R, Smith MA, Fernandez Triana J, Lopez-Vaamonde C, Ratnasingham S, et al. (2010) Molecular Analysis of Parasitoid Linkages (MAPL): gut contents of adult parasitoid wasps reveal larval host. Molecular Ecology in press. 64. Dunne JA, Williams RJ, Martinez ND (2004) Network structure and robustness of marine food webs. Marine Ecology Progress Series 273: 291–302. 65. McCann KS (2007) Protecting biostructure. Nature 446: 29. 66. Ivanova NV, Dewaard JR, Hebert PDN (2006) An inexpensive, automation- friendly protocol for recovering high-quality DNA. Molecular Ecology Notes 6: 998–1002. 67. Ya-Jie J, De-Xing Z, Li-Jun H (2003) Evolutionary conservation and versatility of a new set of primers for amplifying the ribosomal internal transcribed spacer regions in insects and other invertebrates. Molecular Ecology Notes 3: 581–585. 68. Koetschan C, Fo¨rster F, Keller A, Schleicher T, Ruderisch B, et al. (2010) The ITS2 Database III - sequences and structures for phylogeny. Nucleic Acids Research 38: D275–279. 69. Seibel PN, Mu¨ller T, Dandekar T, Schultz J, Wolf M (2006) 4SALE - A tool for synchronous RNA sequence and secondary structure alignment and editing. BMC Bioinformatics 7: 498-. 70. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599. 71. Agapow PM, Crozier RH (2008) Conserve IV . 72. Crozier RH, Agapow PM, Pedersen K (1999) Towards complete biodiversity assessment: an evaluation of the subterranean bacterial communities in the Oklo region of the sole surviving natural nuclear reactor. FEMS Microbiol Ecol 28: 325–334. 73. Yoon I, Williams RJ, Levine E, Yoon S, Dunne JA, et al. (2004) Webs on the Web (WoW): 3D visualization of ecological networks on the WWW for collaborative research and education. Proceedings of the IS&T/SPIE Sympo- sium on Electronic Imaging, Visualization and Data Analysis 5295: 124–132. 74. Ratnasingham S, Hebert PDN (2007) BOLD: The Barcode of Life Data System. Mol Ecol Notes 7: 355–364. PLoS ONE | www.plosone.org 12 July 2011 | Volume 6 | Issue 7 | e14424