200b and miR-200c but not miR-200a reduced the size of prostaspheres. Found at: doi:10.1371/journal.pone.0012445.s003 (1.66 MB TIF) Figure S3 Binding sites of miR-200b and miR-200c in 39UTR of Lin28B or Bmi1 mRNA. Conserved, predicted binding sites for the seed sequences of miR-200b and mir-200c in the 39UTR of Lin28B or Bmi1 mRNA were shown. Found at: doi:10.1371/journal.pone.0012445.s004 (3.36 MB TIF) Figure S4 Let-7 regulated the self-renewal of PC3 PDGF-D. (A) Transfection of pre-let-7 or combination of pre-let-7 and Lin28B siRNA reduced the size of prostaspheres. (B) Transfection of pre- let-7 or combination of pre-let-7 and Lin28B siRNA increased the numbers of prostaspheres with smaller size (30–50 mm) and reduced the number of prostaspheres with bigger size (.100 mm) compared to transfection with control. **, p,0.01 compared to control (Con: control, a: pre-let-7a, abd: combination of pre-let-7a, pre-let-7a, pre-let-7d, al: combination of pre-let-7a and Lin28B siRNA, Lin28B-si: Lin28B siRNA). Found at: doi:10.1371/journal.pone.0012445.s005 (1.75 MB TIF) Table S1 Fold change in EMT-associated genes induced by over-expression of PDGF-D in PC3 cells. Found at: doi:10.1371/journal.pone.0012445.s006 (0.03 MB DOC) Table S2 Fold change in the expression of genes associated with stem-like cells in PDGF-D over-expressing PC3 cells compared to PC3 Neo cells. References 1. Visvader JE, Lindeman GJ (2008) Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat Rev Cancer 8: 755–768. 2. 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