Dimorphic Stress Hormones Figure 3. Locomotor activity is differentially affected by corazonin neuron function and sex. A: Locomotion was monitored for three 12:12 LD cycles on flies expressing different transgenes in corazonin neurons. Raw data are means +/2 SEM from three replicate experiments. Records begin at lights on (ZT0). B: Quantification of total locomotion. Mean amount of locomotion per ten minute bins were estimated for the genotypes listed plotted with 95% confidence intervals are plotted from three independent experiments. Different letters denote statistical significance from each other (Tukey post-hoc test p,0.05). Locomotor values from females are designated in red, males in blue, and for animals with feminized corazonin neurons are indicated in yellow. Animals in the first group have wild-type corazonin neuronal function (w1118), the second grouping are expressing NaChBac in corazonin neurons (Activation) and the third grouping are expressing the ork channel in corazonin neurons (Silencing). doi:10.1371/journal.pone.0009141.g003 PLoS ONE | www.plosone.org 6 February 2010 | Volume 5 | Issue 2 | e9141