14-3-3h Interacts with rEag1 Figure 7. The effect of 14-3-3h over-expression on rEag1 K+ currents. (A) (Left panel) Representative K+ currents recorded from HEK293T cells expressing rEag1 in the absence or presence of 14-3-3h. HEK293T cells were co-transfected with the cDNAs for rEag1 and myc-vector or myc-14-3-3h in the molar ratio of 1:5. The holding potential was 290 mV. The pulse protocol comprised 300-ms depolarizing test pulses ranging from 290 to +50 mV, with 10-mV increments. (Right panel) Normalized mean K+ current density (at +40 mV) of rEag1 channels in the absence or presence of myc- 14-3-3h. The numbers in the parentheses refer to the number of cells analyzed, and the asterisk denotes significant difference from the rEag1 control PLoS ONE | www.plosone.org 7 July 2012 | Volume 7 | Issue 7 | e41203