Gas2 Inhibits Cell Division Figure 3. Over-expression of either the full-length Gas2 protein or the Gas2 domain alone arrests cell division in Xenopus embryos. (A–D) The Gas2 protein injected cell is relatively larger than non-injected normal dividing cells. Gas2 localizes to the injected cell cortex (arrow in A) and also co-localizes with microtubule network shown in yellow in the merged image D. Bar, 100 mm. (E–H) Cells expressing GFP alone and (I–L) GFP- CH domain are similar in size to the neighboring non-expressing control cells. Cells in anaphase can be recognized by their microtubule morphology (arrows in F and J) and separating chromosomes (arrows in G and K). Bars in E–H, 10 mm and bars in I–L, 20 mm. (M–P) Cells expressing full-length GFP-Gas2 and (Q–T) GFP-Gas2 domain expressing cells are relatively larger than neighboring non-expressing cells and they also have multiple nuclei, indicating a failure in cell division (arrows in O and S). Bars, 20 mm. doi:10.1371/journal.pone.0024698.g003 PLoS ONE | www.plosone.org 7 September 2011 | Volume 6 | Issue 9 | e24698