Signature in Renal Carcinoma Figure 6. Osteogenic differentation in clear cell renal cell carcinoma. a. Cellular differentiation experiments showing that KIJ-308 and KIJ- 265 ccRCC cells are capable of osteogenic differentiation in osteogenic media by developing calcium deposits, as shown by Alizarin Red stain. Normal patient-matched cells were unable to differentiate. b. A498 ccRCC cells are also capable of osteogenic differentiation, as shown by Alizarin Red stain, unlike normal renal canine MDCK cells. doi:10.1371/journal.pone.0010696.g006 PLoS ONE | www.plosone.org 9 May 2010 | Volume 5 | Issue 5 | e10696