HSL Null Mice Figure 9. Altered gene expression in white adipocytes from HSL mice. mRNA level of UCP-1 (A), CPT1 (B), pRb (C), RIP140 (D) and PGC1a (E) analyzed with real-time quantitative PCR in isolated adipocytes from periovarial WAT from 2 months old, wildtype and HSL mice fed HFD for 4 weeks (n = 6). Values given are mean6SEM, * p,0.05, ** p,0.01, analyzed with Mann-Whitney U tests. doi:10.1371/journal.pone.0001793.g009 Figure 10. HFD-fed HSL mice have increased oxygen consumption in WAT. Oxygen consumption (A) and level of uncoupling of mitochondria (B) measured using a Clark electrode in periovarial WAT pieces from 10–11 months old, wildtype (n = 11 and 9 respectively) and HSL mice (n = 11 and 8 respectively) fed HFD for 6 months. Values given are mean6SEM, *** p,0.001, analyzed with Mann-Whitney U tests. doi:10.1371/journal.pone.0001793.g010 PLoS ONE | www.plosone.org 8 2008 | Volume 3 | Issue 3 | e1793