RAB-5 Regulates PAR-6 Cortical Dynamics Figure 5. RAB-5 controls actin and PAR-6 organization at the cortex. (A) Images of dMoe::GFP at the cortex of control(RNAi), rab-5(RNAi), dyn- 1(RNAi) and rab-5(RNAi); dyn-1 (RNAi) embryos during the phase of maintenance of polarity. Regions of low fluorescence intensity (LFI) are visible in all backgrounds (white arrows), but quantitation reveals that they are more frequent in rab-5(RNAi) and rab-5(RNAi); dyn-1(RNAi) embryos (red star, compared to control(RNAi): p = 0.00027 and p = 0.0054 respectively, Student’s t-test). n, number of embryos analyzed. Error bars represent standard error of the mean. (B) Images of dMoe::GFP at the cortex of control(RNAi) and rab-5(RNAi) embryos during the phase of maintenance of polarity. Timed quantitation by kymograph analysis shows that the LFI regions are visible for a longer time in rab-5(RNAi) embryos (red star, p = 0.037, Student’s t-test). n, number of embryos analyzed. Error bars represent standard error of the mean. (C) Images of PAR-6::mCherry and dMoe::GFP at the cortex of control(RNAi) and rab-5(RNAi) embryos during the phase of maintenance of polarity. The box is magnified four-fold in inset. Fluorescence intensity was measured along the line in each inset and represented for PAR-6 (red) or dMoe (green). The black arrow in the graph points to a region PLoS ONE | www.plosone.org 8 April 2012 | Volume 7 | Issue 4 | e35286