dsRNA-Mediated Nitric Oxide Response Figure 5. NF-kB contributes to iNOS induction and subsequent antiviral protection against HSV-1 in IRF32/292/2 MEFs. (A) The effective concentration of Bay 11-7082 to inhibit NF-kB translocation to the nucleus after treatment with poly I:C was determined. The effect of NF-kB inhibition by Bay 11-7082 on iNOS mRNA accumulation was measured by qRT-PCR in poly I:C-treated WT (B) and IRF32/292/2 (C) MEFs. Initiation of HSV-1gfp replication was quantified in WT (D) and IRF32/292/2 (E) MEFs following a 5 hour poly I:C treatment in the presence or absence of Bay 11- 7082 and fluorescence compared to untreated monolayers, where fluorescence was set at 100% in each experiment. A 1-way ANOVA with a Tukey post-test was performed to compare efficacy of a range of NF-kB inhibitor concentrations. An unpaired t-test was performed to compare the antiviral PLoS ONE | www.plosone.org 7 February 2012 | Volume 7 | Issue 2 | e31688