Cellular Trafficking of FX-Ad5fiber35 Complex Figure 4. SPR analysis of the in vitro binding of chimeric HAdV5F35 vector to (A) surface-immobilized FX, or (B, C) immobilized HS with or without FX or FXGL. (A), Representative sensorgrams for HAdV5wt vector (discontinuous lines) injected at 26109 and 46109 vp/ml, or for HAdV5F35 injected at the same doses (solid lines). (B), Comparison of binding to HS of HAdV5wt and HAdV5F35 vector particles (26109 vp/ml) in the presence of FX or FXGL at 720 copies per vector particle. Controls shown are FX and FXGL alone. For better clarity, the sensorgrams for virions alone, which superimposed those of FX and FXGL, are not shown (refer to Fig. 1C). (C), Dose-response effect of FX on HAdV5F35 binding to immobilized HS. Note that a detectable signal was observed for 120 copies of FX per virion, and reached the maximal value for 480 copies/vp. doi:10.1371/journal.pone.0018205.g004 PLoS ONE | www.plosone.org 7 May 2011 | Volume 6 | Issue 5 | e18205