GlcAT-P in Nervous System Development Figure 2. Analysis of GlcAT-P expression as revealed by GlcAT-P-GAL4 driven reporter. GlcAT-P is expressed in the larval brain lobes (A–G), the VNC (H–P) and peripheral nerves (Q–S) in the L3 larva brain. lacZ reporter expression in the brain lobes (red, A) colabeled with a-Repo (green, B). (C) Merged frames showing that GlcAT-P is expressed in a subset of Repo-positive glial cells (yellow; arrows). lacZ reporter expression in the mushroom bodies (red, D) colabeled with the neuronal marker Elav (green, E). Merged frame (F) showing co-staining of GlcAT-P and Elav in Kenyon cells (yellow; arrows). (G) Membrane targeted reporter (UAS-mCD8-mCherry) reveals expression of GlcAT-P in Kenyon cell neurites. lacZ reporter expression (H, K) in the larval VNC double labeled with a-Repo (I, J) or a-Elav (L, M). GlcAT-P is expressed in a subset of glial cells (yellow, arrows in J). GlcAT-P expression is also found in about two Elav positive neurons in ventral midline positions (yellow, arrows in M). (N–P) UAS-mCD8-mCherry reporter expression reveals that two cells (white arrow in N) bifurcate (arrowheads) and extend motor axons (blue arrows) bilaterally into the periphery. (P). GlcAT-P.lacZ reporter expression (Q) in cells on peripheral nerves are shown. These cells are peripheral glia as they colabel with Repo (R; yellow and arrows in S). A–F, H–J and K–M show individual frames of 1 mm thickness. G and N are maximum projections of Z-stacks. Bars in A, S: 50 mm. Bars in F, G: 10 mm. doi:10.1371/journal.pone.0028106.g002 PLoS ONE | www.plosone.org 5 November 2011 | Volume 6 | Issue 11 | e28106