ABC Transporters in Neurogenesis Figure 6. ABC transporters are important for neurogenic functions during disease states as revealed by the CCI paradigm. The proliferation capacity measured by BrdU incorporation (A) was significantly decreased in the induced hemisphere of all ABC transporter-deficient strains (ABCB10/0: 247%; ABCG20/0: 231%; ABCC10/0: 253%) (right). The number of Sox2+ cells was not significantly altered in the induced brain hemisphere (B), but shows a trend toward a decreased number in ABCB1- and ABCC1- deficient mice that resembles the decreases in immature DCX+ and calretinin+ neurons (C, D). The number of DCX+ neuronal progenitors (B) was significantly reduced in the induced hemisphere of ABCB1- (236%) or ABCC1-deficient (233%) animals as compared to controls (right). Additionally, the number of early granule cells expressing calretinin (D) was decreased in the induced hemisphere of ABCB1- (29%) and ABCC1-deficient mice (224%) (right), while there was no change in ABCG2-deficient mice. Error bars: SEM, *p,0.05; **p,0.01; ***p,0.001. doi:10.1371/journal.pone.0035613.g006 PLoS ONE | www.plosone.org 7 April 2012 | Volume 7 | Issue 4 | e35613