Calbindin-D32k in the Echinoderm Nervous System Figure 6. Alignment of the peptide sequences and phylogenetic trees of members of the calbindin subfamily from different species. (A) Sequence alignment of rat calbindin 2 (NP_446440.1), Drosophila calbindin-D32k (NP_476838.1), S. kowalevskii calbindin-D32k (XP_002735965.1), and S. purpuratus calbindin-D32k proteins (XP_781517.2). Alignment of the proteins was made using the CLUSTALW multiple sequences alignment. Dots represent conserved residues and black lines below the residues represents the presence of an EF hand domain. (B) Phylogenetic tree of members of the calbindin subfamily from different species. Calb32_Dm Drosophila melanogaster (NP_476838.1), Calb32_Sk S. kowalevskii (XP_002735965.1), Calb32_Sp S. purpuratus (XP_781517.2), Calb32_Sm S. mansoni (XP_002574332.1), Calb2_Gg G. gallus (NP_990647.1), Calb2_Dr D. rerio (NP_957005.1), Calb2_Rn R. norvegicus (NP_446440.1), Calb2_Hs H. sapiens (NP_001731.2), Calb1_Xl X. laevis (NP_00108408.1), Calb1_Gg G. gallus (NP_990844.1), Calb1_Mm M. musculus (AAH16421.1), Calb1_Rn R. norvegicus (AAH81764.1), Calb1_Hs H. sapiens (NP_004920.1), SCGN_Sk S. kowalevskii (NP_001161653.1), SCGN_Sp S. purpuratus (XP_785060.2), SCGN_Dr D. rerio (NP_001005776.1), SCGN_Xl X. laevis (NP_001088097.1), SCGN_Rn R. norvegicus (NP_963855.1), SCGN_Mm M. musculus (NP_663374.1). The phylogenetic tree was constructed from an alignment created by using the unweighted pair group method with arithmetic mean. The scale bar represents 100 times the expected number of amino acid substitution. doi:10.1371/journal.pone.0032689.g006 PLoS ONE | www.plosone.org 9 March 2012 | Volume 7 | Issue 3 | e32689