Inhibition of Chlamydial Infection by Ivermectin Figure 2. Ivermectin inhibits the development of chlamydial inclusions in epithelial cells. HeLa cells grown on glass coverslips were infected with C. trachomatis serovar L2 at an MOI of 1, followed by treatment with ivermectin at the indicated concentrations at 1 hour post infection (hpi). At 24 hpi, cells were fixed with ice cold methanol for 10 minutes, followed by staining with C. trachomatis genus antibodies (Argene) and Hoechst (Sigma), and observed on a widefield fluorescence microscope (Leica). doi:10.1371/journal.pone.0048456.g002 PLOS ONE | www.plosone.org 3 October 2012 | Volume 7 | Issue 10 | e48456