IL-36a and Pulmonary Inflammation Figure 7. IL-36a induced the expression of T cell costimulatory molecules in splenic CD11c+ cells. A–C) Transcript expression of the co- stimulatory molecules CD80, CD86 and CD40 in splenic CD11c+ cells 2 h following incubation with increasing concentrations of IL-36a. Transcript expression was evaluated by SYBR-Green based quantitative real-time PCR. *Indicates significant differences (P,0.05) compared to 0 mg/mL group. Data represent mean6SD from quadruplicate samples from one of two representative experiments. D) Flow cytometric evaluation of splenic CD11c+ cells 24 h following incubation with increasing concentrations of IL-36a for 2 h. E) Cell surface expression of co-stimulatory molecules in splenic CD11c+ cells 24 h following incubation with increasing concentrations of IL-36a for 2 h. MFI – mean fluorescence intensity. *Indicates significant differences (P,0.05) compared to 0.1 mg/mL group. Data represent mean6SD from triplicate samples from one of two representative experiments. doi:10.1371/journal.pone.0045784.g007 PLOS ONE | www.plosone.org 12 September 2012 | Volume 7 | Issue 9 | e45784