UTF1 and Cervical Carcinogenesis Figure 4. UTF1 expression increases during cervical carcinogenesis. A) Expression of UTF1 mRNA in liquid-based cytological samples. Expression was assessed by quantitative RT-PCR. Ecto, normal ectocervix; LSIL, low grade squamous intraepithelial lesion; HSIL, high grade squamous intraepithelial lesion; SCC, squamous cell carcinoma. B) Immunohistochemical staining of UTF1 protein in cervical tissue samples, magnification 6200. C) semi-quantitative analysis of staining. Testis, positive control; Neg, negative control without primary antibody. *, p,0.05; **, p,0.01 (Kruskal-Wallis followed by Dunn’s Multiple Comparison test). doi:10.1371/journal.pone.0042704.g004 Figure 5. Expression of UTF1 and its regulators Oct4A and Sox2 in cervical cancer cell lines. A) Quantitative RT-PCR. B) Western Blot. C) Immunofluorescence; magnification 6400. Results are representative of 3 independent experiments. *, p,0.05 vs NCCIT+RA; ***, p,0.001 vs NCCIT+RA (Unpaired t test). doi:10.1371/journal.pone.0042704.g005 PLoS ONE | www.plosone.org 6 August 2012 | Volume 7 | Issue 8 | e42704