5T4 Regulates CXCR4 Chemotaxis Figure 1. Differentiating mES cells show 5T4 dependent CXCL12 chemotaxis. (A), Triplicate quantitative RT-PCR of WT-ES cells after 3 days differentiation with significant changes in 5T4, CD26 and CXCL12 mRNA but not CXCR4 respectively P = 0.014, 0.057, 0.81, 0.012 by Student’s t-test. (B), Flow cytometry analysis of WT-ES cell differentiation 5T4 (triangles), CD26 (circles) and SSEA-1 (diamonds) (n.3 a single representative time PLoS ONE | www.plosone.org 3 April 2010 | Volume 5 | Issue 4 | e9982