Myelin Induces Inflammation Figure 1. Inflammatory effect of myelin in peritoneal cavity from WT and CR3 KO mice. (A) Morphology of infiltrated cells in peritoneal cavity from WT mice. Neutrophils were indicated with black arrow and macrophages were indicated with white arrow. (Scale bar = 50 mm). (B) The total number of cells in peritoneal cavity. (C) Number of infiltrated neutrophils and macrophages in peritoneal cavity. (D) Levels of TNF-a and IL-1b in peritoneal lavage fluid detected by ELISA. Data were expressed as mean 6 SEM. (n = 4 at each time point, *P,0.05 vs. WT + PBS; #P,0.05 vs. CR3 KO + Myelin; t-test). doi:10.1371/journal.pone.0009380.g001 PLoS ONE | www.plosone.org 4 February 2010 | Volume 5 | Issue 2 | e9380