rodent-specific loci containing (GGCAGG)n repeat motif showed germ-line mutation rates up to 8.861022 per gamete [33]. Direct records of new length variants identified in comparisons between parents and offspring loci may be considered the most unambiguous way for analyzing mutational process in the germ line [27,29]. Unisexual reptiles reproduce clonally and have a low level of recombinational events, thus all of the observed changes in microsatellite cluster are probably mutations. In three out of four D. unisexualis families the patterns of mutations were similar in all offspring, suggesting that mutation must be occurring in the mitotic creation of germ-line tissue, such that multiple oocytes would carry the same mutation.. The offspring of one family (offspring 3, family 4) showed different pattern of mutations: in most offsprings the mutant allele arose following the loss of a monomer, while in one offspring the imperfect monomer GATATA was lost. 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