VEGFA and miR-361-5p Expression in Human SCC Figure 2. Impact of altered miRNA-361-5p levels on secretion of endogenous VEGFA. A431 and HaCaT cells were transfected with the indicated concentrations of miR-361-5p mimics, antisense inhibitors, or controls, either in the presence (A, B) or absence (C, D) of TNF-a. Cellular supernatants were analyzed for VEGFA protein content using a human VEGFA ELISA. Three independent experiments were performed in triplicate. Mean values 6 S.D. from a representative experiment are plotted. Two-tailed, unpaired t-tests were used to calculate P values (one and two asterisks denote P values,0.05 and,0.01, respectively). doi:10.1371/journal.pone.0049568.g002 PLOS ONE | www.plosone.org 5 November 2012 | Volume 7 | Issue 11 | e49568