Myelin Induces Inflammation Figure 9. Effect of inactivation of PI3K and Akt on myelin-induced cytokine expression in macrophages. Macrophages were pre-treated with LY-294002 (A) or Akt inhibitor IV (B) for 30 minutes and then treated with myelin for 12 hours. Cytokines at transcriptional level were detected by qRT-PCR. Data were expressed as mean 6 SEM and similar results were obtained from three independent experiments. (n = 3, *P,0.05 vs. control; #P,0.05 vs. inhibitor treatment; t-test). doi:10.1371/journal.pone.0009380.g009 PLoS ONE | www.plosone.org 12 February 2010 | Volume 5 | Issue 2 | e9380