Supporting Information Figure S1 Temperature sensitivity of iG170Dhsp82. (A) Growth kinetics of iG170Dhsp82 at indicated temperatures. This strain shows slow growth phenotype at 37uC. (B) iG170Dhsp82 cells, wild type cells (HSP82), tester strains YDS32 (MATa) and YDS31 (MATa) all show comparable growth at 37uC on YPD plate. (TIF) Figure S2 Dsir2 strain shows de-repression of telomere silencing at YFR057w locus. Semi quantitative RT-PCR shows increase in YFR057w transcript in Dsir2 strain compared to the wild type strain (SIR2). ACT1 transcript level remains comparable in both the strains. (TIF) References 1. Caplan AJ, Jackson S, Smith D (2003) Hsp90 reaches new heights. EMBO Reports 4(2): 126–130. 2. Terasawa K, Minami M, Minami Y (2005) Constantly updated knowledge of hsp90. J Bio Chem (Tokyo) 137: 443–447. 3. Donnelly A, Blagg BSJ (2008) Novobiocin and Additional Inhibitors of the Hsp90 C-Terminal Nucleotide-binding Pocket. Curr Med Chem 15(26): 2702–2717. 4. 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