AMD3100 Enhances Engraftment Figure 6. Post transplant administration of AMD3100 increase donor cell division. A: In vivo cell division measurement. CD45.1+ KTLS cells were labeled with CFSE and injected into lethally irradiated C57BL/6 CD45.2 Thy1.2 mice (76103 KTLS cells/mouse). The 2 panels on the left showed the sorted CFSE-labeled KTLS cells prior to injection to recipient mice. The recipient mice were treated with AMD3100 at 5 mg/kg or PBS every other day beginning at day +2. The mice were sacrificed at day +7 and bone marrow cells measured for CFSE intensity (The 4 panels on the right. Top 2 panels: recipient mice treated with PBS control; Lower 2 panels: recipient mice treated with AMD3100). The CFSE intensity was gated on CD45.1+ donor derived cell population (n = 5 mice in each group). B: In vitro marrow cell proliferation responses to AMD3100 or G-CSF. Marrow cells (56105/well) were cultured with various concentrations of AMD3100 or recombinant G-CSF, and [3H] thymidine incorporation was measured (three separate sets of experiments with triplicates in each experiment). doi:10.1371/journal.pone.0011316.g006 PLoS ONE | www.plosone.org 9 June 2010 | Volume 5 | Issue 6 | e11316