performed with mouse anti-V5 or anti-Xpress (1:250; Invitrogen, USA, CA) and GST-Alexa Fluor 488nm (1:100; Santa Cruz, USA, CA). After that, samples were incubated with secondary antibody, goat anti-mouse-Alexa Fluor 568nm (1:200; Sigma- Aldrich, USA, MO). Cells were subjected to Zeiss LSM510 META confocal microscopy studies. Reverse transcription and Real time PCR iScriptTM cDNA synthesis kit (Bio-Rad, USA, CA) was performed according to manufacturer’s instructions. Quantitative Real time PCR was carried out using iTaqTM SYBRH Green Supermix with ROX (Bio-Rad, USA, CA) according to manu- facturer’s instructions. Primer sequences used for Real time PCR were listed in Table S2. Chromatin immunoprecipitation (ChIP) Confluent HEK293T cells cultured in 10 cm plates were cross- linked with Formaldehyde (37% stock) for 15min at 37uC. The reaction was quenched with 1M glycine for 5min at RT. 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