MicroRNAs of Worms and Flies Table 2. Summary of D. melanogaster miRNA relationships. miRNA Group ID Sequence-Related miRNAs Table 2. cont. miRNA Group ID Sequence-Related miRNAs bantam let-7 miR-2a miR-3 miR-9a miR-10 miR-12 miR-14 miR-31a miR-219 miR-263a miR-275 miR-276a 59 Sequence dme-bantam dme-miR-306* dme-let-7 dme-miR-963 dme-miR-977 dme-miR-984 dme-miR-2a dme-miR-2b dme-miR-2c dme-miR-6 dme-miR-11 dme-miR-13a dme-miR-13b dme-miR-308 dme-miR-3 dme-miR-309 dme-miR-318 dme-miR-9a dme-miR-9b dme-miR-9c dme-miR-12 dme-miR-280 dme-miR-283 dme-miR-289 dme-miR-960 dme-miR-14 dme-miR-316 dme-miR-31a dme-miR-31b dme-miR-219 dme-miR-315 dme-miR-275 dme-miR-306 dme-miR-967 dme-miR-276a dme-miR-276b Full Sequence dme-miR-2b dme-miR-2c dme-miR-13a dme-miR-13b dme-miR-2c dme-miR-13a dme-miR-13b dme-miR-13a dme-miR-13b dme-miR-13b dme-miR-309 dme-miR-318 dme-miR-9b dme-miR-9c dme-miR-9c dme-miR-10 dme-miR-100 dme-miR-31b dme-miR-263a dme-miR-263b dme-miR-276b miR-279 miR-281-2* miR-285 miR-312 miR-954 miR-1003 miR-1006 miR-1009 miR-1010 miR-iab4as-3p miR-iab4as-5p 59 Sequence dme-miR-279 dme-miR-286 dme-miR-996 dme-miR-4 dme-miR-7 dme-miR-79 dme-miR-281-1* dme-miR-281-2* dme-miR-285 dme-miR-995 dme-miR-998 dme-miR-92a dme-miR-92b dme-miR-310 dme-miR-311 dme-miR-312 dme-miR-313 dme-miR-1003 dme-miR-1004 dme-miR-1006 dme-miR-1014 dme-miR-1010 dme-miR-1016 dme-miR-iab4as-5p dme-miR-iab-4-5p Full Sequence dme-miR-281-2* dme-miR-998 dme-miR-998 dme-miR-92b dme-miR-310 dme-miR-312 dme-miR-310 dme-miR-312 dme-miR-311 dme-miR-312 dme-miR-313 dme-miR-313 dme-miR-954 dme-miR-966 dme-miR-1009 dme-miR-1010 dme-miR-iab4as-3p dme-miR-iab-4-3p dme-miR-iab-4-5p 70/152 Drosophila miRNAs can be arranged into 24 groups that have sequence homology at the 59 end (61 miRNAs, listed separately in Dataset S4) and/or over their entire length (38 miRNAs, listed separately in Dataset S5). See Methods and text for explanation of match criteria. The miRNA group ID was chosen as the miRNA closest to the consensus sequence of the grouped related miRNAs. D. melanogaster miRNAs with 60–69.9% similarity over their full sequence are listed in Dataset S6. doi:10.1371/journal.pone.0002818.t002 overlap. Combining the two strategies for identification of homologies among miRNAs that we described above, we identify 84 C. elegans sequence-related miRNAs grouped in 20 families (Table 1). This analysis expands the previously reported number of members in C. elegans miRNA families [43,51,63,64] and establishes 1 new sequence-related group containing miRNAs cel-miR-78 and cel-miR-272. About half (101/211) of the sequence relationships described in this work have not been PLoS ONE | www.plosone.org 4 July 2008 | Volume 3 | Issue 7 | e2818