PPARc Ligands Differentially Impact OVCAR Cells Figure 6. Annexin V assay to detect apoptosis in Ovcar3 cells. Ovcar3 cells were serum starved for 24 hours and treated with vehicle control (DMSO) or 10 mM of CGZ or TGZ for (A) 4 hours, (B) 8 hours, or (C) 12 hours. Results are the means 6 SEM of 3 measurements from three individual experiments. Light gray bars represent viable cells; dark gray bars represent cells undergoing early and late apoptosis as well as those that are dead. Bars that do not share a letter or number designation are significantly different (p,0.05). doi:10.1371/journal.pone.0016179.g006 Figure 7. Cell cycle kinetics using flow cytometry. Ovcar3 cells were serum starved for 24 hours and treated for an additional 24 hours with vehicle control (DMSO) or 10 mM of CGZ or TGZ. (A) G0/G1, (B) G2, (C) S phase. Results are the means 6 SEM for at least 3 measurements from three individual experiments. Bars that do not share a letter or number designation are significantly different (p,0.05). doi:10.1371/journal.pone.0016179.g007 PLoS ONE | www.plosone.org 8 January 2011 | Volume 6 | Issue 1 | e16179