Androgen-Dependent Exons Figure 3. Androgens regulate expression of a number of alternative mRNA isoforms in LNCaP cells. (A) Capillary agarose gel electrophoretic analysis of RT-PCR products amplified from mRNA from cells grown either in steroid-deplete medium (lanes 1–4) or after 24 hours of treatment with 10 nM R1881 (lanes 5–8). The predicted mRNA isoforms detected are shown to the right of each gel exactly as summarised in Figure 2B. (B) Fluorescent quantification was performed to obtain the relative normalised expression ratio, which is the contribution of the PCR product representing the alternative (androgen-regulated) transcript/constitutive transcript or 39 transcript end/59 transcript end. Data from the experimental quadruplicates (gel image) were used to obtain the mean 6 SE. doi:10.1371/journal.pone.0029088.g003 PLoS ONE | www.plosone.org 5 December 2011 | Volume 6 | Issue 12 | e29088