Found at: doi:10.1371/journal.pone.0009593.s005 (0.12 MB TIF) Figure S6 Flow cytometry analysis of apoptotic BMDCs and splenic DCs after LPS challenge (A) BMDC apoptosis after LPS stimulation. BMDCs were stimulated 10 mg/ml LPS for 96 h and then stained with Annexin-V and PI followed by flow cytometry analysis of apoptotic cells. (B) Splenic DC apoptosis after LPS challenge. Jak22/2 and control mice were first challenged with 150 mg/mouse of LPS and were sacrificed after 20 h of injection. Splenic DC apoptosis was then analyzed by flow cytometry as above. Found at: doi:10.1371/journal.pone.0009593.s006 (0.11 MB TIF) References 1. Wen H, Schaller MA, Dou Y, Hogaboam CM, Kunkel SL (2008) Dendritic cells at the interface of innate and acquired immunity: the role for epigenetic changes. J Leukoc Biol 83: 439–446. 2. Steinman RM, Hemmi H (2006) Dendritic cells: translating innate to adaptive immunity. Curr Top Microbiol Immunol 311: 17–58. 3. 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