Wnt5a in AD Figure 4. Wnt5a signaling regulates Ab neurotoxicity. The MTT assay was used to measure cell viability in cortical cultures at 24 hrs after indicated treatments. A. Cell survival rates in cultures challenged with Ab oligomers, H2O2, or a combination of Ab oligomers and H2O2. B. Cultures challenged with Ab (500 nM) and H2O2 (100 mM) in the presence of Wnt5a at different concentrations. C. Cultures challenged with Ab and H2O2 in the presence of anti-Wnt5a or ant-Fz5 antibodies. D. Effects of Foxy5 on the neurotoxicity of Ab/H2O2. E. Effects of Box5 on the neurotoxicity of Ab/ H2O2. Data are expressed as mean 6 SEM (n$12 from three independent experiments). * p,0.05; ** p,0.01; *** p,0.001. # p,0.05 (vs. the group of PLoS ONE | www.plosone.org 5 August 2011 | Volume 6 | Issue 8 | e22920