Human Flt3L Expands Dog DCs Figure 3. Activation status of dog peripheral blood DC cultures. Peripheral blood cultures were collected after 7 days in culture (PBDC culture). Cells were then incubated for additional 24 h without added cytokines in the absence (mock) or presence of CpG2216 or LPS. Activation status was assessed by flow cytometry using an antibody against Major Histocompatibility Complex type II (MHCII, A) or against the costimulatory marker CD80 (B). Black numbers in representative dot plots or histograms indicate the percentage of MHCII+ or CD80+ cells. Green numbers indicate MHCIIhigh cells. Insets on the top right show the dot plot of cells incubated with isotype control mouse IgG1. Insets on the bottom right show PLoS ONE | www.plosone.org 5 June 2010 | Volume 5 | Issue 6 | e11074