Induced MSC Are Not Neurons Figure 2. This panel shows the pattern of neural markers expression in the non-induced, chemically-induced and serum-free MSC groups. Primary neuronal cultures were used as positive controls for the immunocytochemistry. Phase microscopy images of cells stained for: b- tubulin III (b-tubIII), MAP-2, NeuN, NF-200, NSE, S100 and GFAP. Scale bar = 100 mm. doi:10.1371/journal.pone.0005222.g002 PLoS ONE | www.plosone.org 5 April 2009 | Volume 4 | Issue 4 | e5222