CYP5122A1, a Novel Cytochrome P450 of Leishmania Figure 6. In vitro infective abilities of WT and HKOs. A. Photomicrographs of macrophage infected with WT and HKO parasites for different time points. b: 1 h; d: 2 h; f: 4 h; h: 10 h; a, c, e, g: nomarski of b, d, f, h respectively. Scale, 5 mm. P: promastigote, A: amastigote. B. Bar graph showing percentages of macrophages infected with WT and HKO parasites. Mean 6 SE, n = 3, * P#0.05. C. Number of parasites present in infected macrophages over a period of time. Mean 6 SE, n = 3, * P#0.05. doi:10.1371/journal.pone.0025273.g006 PLoS ONE | www.plosone.org 8 September 2011 | Volume 6 | Issue 9 | e25273