14. Vermeer PD, Denker J, Estin M, Moninger TO, Keshavjee S, et al. (2009) MMP9 modulates tight junction integrity and cell viability in human airway epithelia. Am J Physiol Lung Cell Mol Physiol 296: L751–762. 15. Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, et al. (2003) Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100: 14339–14344. 16. Motulsky H (1995) Intuitive Biostatistics. New York: Oxford University Press. 386 p. 17. Lee WS, Kanai Y, Wells RG, Hediger MA (1994) The high affinity Na+/glucose cotransporter. Re-evaluation of function and distribution of expression. J Biol Chem 269: 12032–12039. 18. Zhao FQ, Keating AF (2007) Functional properties and genomics of glucose transporters. Curr Genomics 8: 113–128. 19. Holtug K, Hansen MB, Skadhauge E (1996) Experimental studies of intestinal ion and water transport. Scand J Gastroenterol 216(Suppl): 95–110. 20. Zeiher BG, Eichwald E, Zabner J, Smith JJ, Puga AP, et al. (1995) A mouse model for the delta F508 allele of cystic fibrosis. J Clin Invest 96: 2051–2064. 21. Saumon G, Martet G, Loiseau P (1996) Glucose transport and equilibrium across alveolar-airway barrier of rat. Am J Physiol 270: L183–190. 22. Dawson PA, Mychaleckyj JC, Fossey SC, Mihic SJ, Craddock AL, et al. (2001) Sequence and functional analysis of GLUT10: a glucose transporter in the Type 2 diabetes-linked region of chromosome 20q12-13.1. Mol Genet Metab 74: 186–199. 23. Mcvie-Wylie A (2001) Molecular Cloning of a Novel Member of the GLUT Family of Transporters, SLC2A10 (GLUT10), Localized on Chromosome 20q13.1: A Candidate Gene for NIDDM Susceptibility. Genomics 72: 113–117. 24. Fischer AJ, Goss KL, Scheetz TE, Wohlford-Lenane CL, Snyder JM, et al. (2009) Differential gene expression in human conducting airway surface epithelia and submucosal glands. Am J Respir Cell Mol Biol 40: 189–199. 25. Singh PK, Jia HP, Wiles K, Hesselberth J, Liu L, et al. (1998) Production of beta-defensins by human airway epithelia. Proc Natl Acad Sci U S A 95: 14961–14966. 26. Cuskey SM, Wolff JA, Phibbs PV, Olsen RH (1985) Cloning of genes specifying carbohydrate catabolism in Pseudomonas aeruginosa and Pseudomonas putida. J Bacteriol 162: 865–871. 27. Smith JJ, Travis SM, Greenberg EP, Welsh MJ (1996) Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid. Cell 85: 229–236. 28. Landry JS, Eidelman DH (2001) Airway surface liquid: end of the controversy? J Gen Physiol 117: 419–422. 29. Coleman DL (1973) Effects of parabiosis of obese with diabetes and normal mice. Diabetologia 9: 294–298. 30. Coleman DL (1978) Obese and diabetes: two mutant genes causing diabetes- obesity syndromes in mice. Diabetologia 14: 141–148. 31. Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, et al. (1995) Weight- reducing effects of the plasma protein encoded by the obese gene. Science 269: 543–546. 32. Ingalls AM, Dickie MM, Snell GD (1950) Obese, a new mutation in the house mouse. J Hered 41: 317–318. 33. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, et al. (1994) Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425–432. 34. Hummel KP, Dickie MM, Coleman DL (1966) Diabetes, a new mutation in the mouse. Science 153: 1127–1128. 35. Lessie TG, Phibbs PV (1984) Alternative pathways of carbohydrate utilization in pseudomonads. Annu Rev Microbiol 38: 359–388. Airway Glucose and Innate Immunity 36. Rakhimova E, Munder A, Wiehlmann L, Bredenbruch F, Tummler B (2008) Fitness of isogenic colony morphology variants of Pseudomonas aeruginosa in murine airway infection. PLoS ONE 3: e1685. 37. Uldry M, Thorens B (2004) The SLC2 family of facilitated hexose and polyol transporters. Pflugers Arch 447: 480–489. 38. Bento JL, Bowden DW, Mychaleckyj JC, Hirakawa S, Rich SS, et al. (2005) Genetic analysis of the GLUT10 glucose transporter (SLC2A10) polymorphisms in Caucasian American type 2 diabetes. BMC Med Genet 6: 42. 39. Lin WH, Chuang LM, Chen CH, Yeh JI, Hsieh PS, et al. (2006) Association study of genetic polymorphisms of SLC2A10 gene and type 2 diabetes in the Taiwanese population. Diabetologia 49: 1214–1221. 40. Mohlke KL, Skol AD, Scott LJ, Valle TT, Bergman RN, et al. (2005) Evaluation of SLC2A10 (GLUT10) as a candidate gene for type 2 diabetes and related traits in Finns. Mol Genet Metab 85: 323–327. 41. Pe´rez-D´ıaz J, Mart´ın-Requero A, Ayuso-Parrila MS, Parrilla R (1977) Metabolic features of isolated rat lung cells. I. Factors controlling glucose utilization. Am J Physiol 232: E394–400. 42. Morens DM, Taubenberger JK, Fauci AS (2008) Predominant role of bacterial pneumonia as a cause of death in pandemic influenza: implications for pandemic influenza preparedness. J Infect Dis 198: 962–970. 43. Ampofo K, Bender J, Sheng X, Korgenski K, Daly J, et al. (2008) Seasonal invasive pneumococcal disease in children: role of preceding respiratory viral infection. Pediatrics 122: 229–237. 44. Philips BJ, Meguer JX, Redman J, Baker EH (2003) Factors determining the appearance of glucose in upper and lower respiratory tract secretions. Intensive Care Med 29: 2204–2210. 45. Kalsi KK, Baker EH, Fraser O, Chung YL, Mace OJ, et al. (2009) Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism. Pflugers Arch 457: 1061–1070. 46. Coucke P, Willaert A, Wessels M, Callewaert B, Zoppi N, et al. (2006) Mutations in the facilitative glucose transporter GLUT10 alter angiogenesis and cause arterial tortuosity syndrome. Nat Genet 38: 452–457. 47. Coucke PJ, Wessels MW, Van Acker P, Gardella R, Barlati S, et al. (2003) Homozygosity mapping of a gene for arterial tortuosity syndrome to chromosome 20q13. J Med Genet 40: 747–751. 48. Wessels MW, Catsman-Berrevoets CE, Mancini GM, Breuning MH, Hoogeboom JJ, et al. (2004) Three new families with arterial tortuosity syndrome. Am J Med Genet A 131: 134–143. 49. Reading PC, Allison J, Crouch EC, Anders EM (1998) Increased susceptibility of diabetic mice to influenza virus infection: compromise of collectin-mediated host defense of the lung by glucose? J Virol 72: 6884–6887. 50. Ritchings BW, Almira EC, Lory S, Ramphal R (1995) Cloning and phenotypic characterization of fleS and fleR, new response regulators of Pseudomonas aeruginosa which regulate motility and adhesion to mucin. Infect Immun 63: 4868–4876. 51. Beatson SA, Whitchurch CB, Semmler AB, Mattick JS (2002) Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa. J Bacteriol 184: 3598–3604. 52. de Groot A, Koster M, Ge´rard-Vincent M, Gerritse G, Lazdunski A, et al. (2001) Exchange of Xcp (Gsp) secretion machineries between Pseudomonas aeruginosa and Pseudomonas alcaligenes: species specificity unrelated to substrate recognition. J Bacteriol 183: 959–967. 53. McCaw ML, Lykken GL, Singh P, Yahr TL (2002) ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon. Mol Microbiol 46: 1123–1133. 54. Stoltz DA, Ozer EA, Taft PJ, Barry M, Liu L, et al. (2008) Drosophila are protected from Pseudomonas aeruginosa lethality by transgenic expression of paraoxonase-1. J Clin Invest 118: 3123–3131. PLoS ONE | www.plosone.org 8 January 2011 | Volume 6 | Issue 1 | e16166