Gene name Forkhead box A1 Cyclin E1 Peroxiredoxin 4 Keratin 17 Keratin 5 c-mer proto-oncogene tyrosine kinase Estrogen receptor 1 (alpha) ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c Frizzled homolog 7 Chemokine (C-X-C motif) ligand 1 Mediator complex subunit 1 Acyl-CoA synthase long-chain family member 1 Phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) Thioredoxin reductase 1 Thioredoxin 1 18S RNA Milk fat globule-EGF factor 8 protein MFGE8 NM_001045489 278–444 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 ERBB2 NM_001003817 2980–3162 Gene symbol FOXA1 MERKT ESR1 ATP5G KRT17 KRT5 FZD7 CXCL1 MED1 ACSL1 PIK3R1 TXN1 RN18S CCNE1 NM_007633 160–330 PREDX4 NM_016764 716–883 NM_008587 1944–2127 NM_007956 1560–1752 NM_007506 167–223 NM_010663 679–851 NM_027011 1005–1170 NM_008057 1477–1631 NM_008176 179–352 NM_013634 1459–1623 NM_007981 1586–1759 NM_001077495 1906–2080 TXNRD1 NM_00142523 1587–1763 NM_011660 315–484 NR_003278 1194–1294 Table 4. List of primers used for real time quantitative PCR analysis. Protein tyrosine phosphatase 4a2 PTP4A2 NM_008974 427–598 Accession IDs Sequence range Product size (bp) NM_008259 456–588 doi:10.1371/journal.pone.0004968.t004 mole of free TNB with the extinction coefficient 13.6 mM21 cm21. For accessing the activity of Trx, assays were performed as above with the similar reaction cocktail except that 600 nM rat TrxR1 but not Trx1 was included. Reaction solutions without TrxR1 were used as the control. Data analysis and statistics All experiments were performed with six to eight samples per group, and all results were derived from at least three independent experiments. Data are shown as mean values 6standard deviation (SD). Comparison between groups was done using Student’s unpaired t-test. Tumor latency was analysed using a Kaplan- Meier survival analysis followed by log rank tests. In all statistical comparisons, P,0.05 was used to indicate a significant difference. Note that for the ex vivo and in vitro experiments, while tumor cells derived from male and female mice showed similar characteristics in both FVB/N and C57BL/6J backgrounds, only results derived from the tumor cells of female FVB/N mice were shown. PLoS ONE | www.plosone.org 14 March 2009 | Volume 4 | Issue 3 | e4968 133 172 185 193 57 171 118 173 166 167 155 174 183 165 174 175 177 170 101 Adiponectin and Breast Cancer Primer sequences (F) 59-GAA GGG CTC CTG TGC TAG TT-39 (R) 59-AGG ACA TGT TGA AGG AAG CC-39 (F) 59-GAA GGG CTC CTG TGC TAG TT-39 (R) 59-TGC CCA TTG GTA TCT CTG AA-39 (F) 59-AAG CAG CAT GCA TGA AAG AC-39 (R) 59-TGC AGG TGA ATG TAC TTG GG-39 (F) 59-CCG GAG TGT ACA CGT TTC TG-39 (R) 59-TTG TTA CTC ATG TGC CGG AT-39 (F) 59-GGG AAT TCC AGA CCA GTG TC-39 (R) 59-TTG AGA GAT GGG TTC CTG GC-39 (F) 59-ACA GCT TCG GGT CTG AGT TC-39 (R) 59-GGC AAT TTC TTC ATC TGG GT-39 (F) 59-CGA TGA CAA AGG AGT CCT GA-39 (R) 59-GCT GGA TCT GGG ATT ATT GT-39 (F) 59-AAG AAG AAC CAC GAG GAG GA-39 (R) 59-AAG AAC CAG TCT TCG GCA TC-39 (F) 59-GCA GAC ACA CGT CTC TGA CA-39 (R) 59-TTG CAG CTC CTC ATA CTT GG-39 (F) 59-AGA CTG AGA GAG GAC CAT GC-39 (R) 59-CAT GCC CAG CTG TGT AGA AC-39 (F) 59-TTC CTA GGT GAG CGT GAC TG-39 (R) 59-TAG GTG AGC ACC GTG AAG AG-39 (F) 59-ACC CAA ACC GAA GTC ATA GC-39 (R) 59-GTT GTC AGA AGC CAG CGT T-39 (F) 59-ATT TGC TGG AGA AGG GAG AA-39 (R) 59-AGT CCT CGT TCT GGA TGA CC-39 (F) 59-CAG ACC TTG GAG TGA AAC CA-39 (R) 59-GAG CCC AGT CCA TTC TGT CT-39 (F) 59-CTA TGA AGG CTA CGG ACA GA-39 (R) 59-CCT TTC ACA CAC ACC TCA CC-39 (F) 59-TCC AAA TAC CAG CAG GAT CA-39 (R) 59-ATG CTT CGA TAG CCG TTC TT-39 (F) 59-TTG GAA TAT GGC TGT TGT GG-39 (R) 59-CAC GAC ACG TTC ATC GTC TT-39 (F) 59-AAG CCC TTC TTC CAT TCC CT-39 (R) 59-CCT TGT TAG CAC CGG AGA AC-39 (F) 59-TAA AGG AAT TGA CGG AAG GG-39 (R) 59-CTG TCA ATC CTG TCC GTG TC-39