Table S2 Annual fruit stage of Breynia fruticosa and Epicephala rostrata, and life history of E. lativalvaris and E. mirivalvata in Tianzhu Mountain and Wanshi Botanical Garden of Xiamen, China. (DOC) Table S3 Statistics of oviposition scars made by Epicephala lativalvaris on Breynia fruits at five locations. (DOC) Table S4 Statistics of eggs laid by Epicephala mirivalvata on Breynia female flowers at three locations. (DOC) Coevolution between Two Epicephala and Two Breynia Table S7 Developed set of Breynia female flowers in hand- pollination experiment. (DOC) Acknowledgments We appreciate Fuchen Shi for identifying Breynia plants, to Yanru Yin for her assistance in the field. Special thanks are given to members of Tianzhu Mountain National Forest Park and Yingge Mountain Nature Reserves for their support and kind help in the field. This research was supported by the National Natural Science Foundation of China (No. 30930014). GenBank accession number of Epicephala moths COI Author Contributions Table S5 sequences. (DOC) References Table S6 GenBank accession number of Phyllantheae plants matK sequences. (DOC) 1. Janzen DH (1979) How many babies do figs pay for babies? Biotropica 11: 48– 50. 2. Weiblen GD (2002) How to be a fig wasp. Annual Review of Entomology 47: 299–230. 3. Kato M, Takimura A, Kawakita A (2003) An obligate pollination mutualism and reciprocal diversification in the tree genus Glochidion (Euphorbiaceae). Proceed- ings of the National Academy of Science 100 (9): 5264–5267. 4. Pellmyr O (2003) Yuccas, yucca moths, and coevolution: a review. Annals of the Missouri Botanical Garden 90: 35–55. 5. Ehrlich PR, Raven PH (1964) Butterflies and plants: a study in coevolution. Evolution 18: 586–608. 6. Wiebes JT (1979) Co-evolution of figs and their insect pollinators. Annual Review Ecology and Systematics 10: 1–12. 7. Pellmyr O, Leebens-Mack J, Huth CJ (1996) Non-mutualistic yucca moths and their evolutionary consequences. Nature 380: 155–156. 8. Kawakita A, Kato M (2006) Assessment of the diversity and species specificity of the mutualistic association between Epicephala moths and Glochidion trees. Molecular Ecology 15: 3567–3582. 9. Kawakita A, Kato M (2009) Repeated independent evolution of obligate pollination mutualism in the Phyllantheae2Epicephala association. Proceedings of the Royal society B-Biological Sciences 276 (1656): 4172426. 10. Kawakita A (2010) Evolution of obligate pollination mutualism in the tribe Phyllantheae (Phyllanthaceae). Plant Species Biology 25: 3219. 11. Kawakita A, Kato M (2004) Obligate pollination mutualism in Breynia (Phyllanthaceae): further documentation of pollination mutualism involving Epicephala moth (Gracillariidae). American Journal of Botany 91(9): 1319–1325. 12. Okamoto T, Kawakita A, Kato M (2007) Interspecific variation of floral scent composition in Glochidion and its association with host-specific pollinating seed parasite (Epicephala). Journal of Chemical Ecology 33: 106521081. 13. Svensson GP, Okamoto T, Kawakita A, Goto R, Kato M (2010) Chemical ecology of an obligate pollination mutualism: testing the ‘private channel’ hypothesis in the Breynia2Epicephala association. New Phytologist 186: 99521004. Conceived and designed the experiments: HL SW. Performed the experiments: JZ BH XY ZW. Analyzed the data: JZ HL. Wrote the paper: JZ SW HL. 14. Janzen DH (1980) When is it coevolution. Evolution 34(3): 611–612. 15. Machado CA, Robbins N, Gilbert MTP, Herre EA (2005) Critical review of host specificity and its coevolutionary implications in the fig/fig–wasp mutualism. Proceedings of the National Academy of Sciences USA (suppl. 1): 6558–6565. 16. Li BT, Gilbert MG (2008) Flora of China. Missouri Botanical Garden Press. USA 11: 635 pp. 17. Zhang J, Hu BB, Wang SX, Li HH (2012) Six new species of the genus Epicephala Meyrick, 1880 (Lepidoptera: Gracillariidae) associated with Euphorbiaceae plants. Zootaxa 3275: 43–54. 18. Corlett RT (1993) Reproductive phenology of Hongkong shrubland. Journal of Tropical Ecology 9(4): 5012510. 19. Doyle JJ, Doyle L (1987) A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochemical Bulletin 19: 11215. 20. Park DS, Suh S-J, Oh HW, Hebert PDN (2010) Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primes. BMC genomics 11: 423. 21. Samuel R, Kathriarachchi H, Hoffmann P, Barfuss MH, Wurdack KJ, et al. (2005) Molecular phylogenetics of Phyllanthaceae: evidence from plastid MATK and nuclear PHYC sequences. American Journal of Botany 92(1): 1322141. 22. Brower AVZ (1994) Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences 91: 649126495. 23. Caterino MS, Sperling FA (1999) Papilio phylogeny based on mitochondrial cytochrome I and II genes. Molecular Phylogenetics and Evolution 11(1): 1222137. 24. Kathriarachchi H, Samuel R, Hoffmann P, Mlinarec J, Wurdack KJ, et al. (2006) Phylogenetics of the tribe Phyllantheae (Phyllanthaceae; Euphoriaceae sensu lato) based on nrITS and plastid matK DNA sequence data. American Journal of Botany 93(4): 6372655. 25. Swofford DL (2005) PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer. Sunderland. Massachusetts. USA. PLoS ONE | www.plosone.org 9 July 2012 | Volume 7 | Issue 7 | e41657