Wolbachia Infection in Cultured Insect Germlines Figure 2. FISH images of wAlbB infection in insect ovaries captured using a confocal microscope for An. gambiae (A–C), An. stephensi (D–F), Ae. aegypti (G–H) C. tarsalis (J–L) and D. melanogaster (M–O). Images in the left column are the red channel only (Wolbachia), images in the center column are blue channel only (host nuclei) and images in the right column are the merged image (Red: Wolbachia, Blue: host nuclei) The Z-stacks of the ovaries are available as supplementary videos (Movie S1, S2, S3, S4, S5). The scale bar represents 20 mm. Images are compiled from compression of Z stack with a depth of 5 mm. doi:10.1371/journal.pone.0036277.g002 PLoS ONE | www.plosone.org 3 April 2012 | Volume 7 | Issue 4 | e36277