S-Aspirin Protects Aspirin-Induced Gastric Injury Figure 6. Effect of ACS14 on protein expressions of p22phox, p47phox, p67phox and gp91phox protein expressions of gastric tissue in Asp-treated rats. Data are presented as means 6 SE. n = 4. *P,0.05 compared with control; #P,0.05 compared with Asp; {P,0.05 compared with Asp+ACS14 1 mg/kg. doi:10.1371/journal.pone.0046301.g006 mechanism underlying the ACS14-increased gastric blood flow may involve opening of KATP channels. This is supported by a previous study which showed that systemically application of exogenous H2S increased gastric mucosal blood flow by activation of KATP channels [50]. In conclusion, we demonstrated in the present study ACS14, an H2S releasing Asp, protects gastric mucosa against Asp induced injury via inhibition of oxidative stress and increasing blood flow locally. Author Contributions Conceived and designed the experiments: JSB XYW CDY. Performed the experiments: LL JC CJS. Analyzed the data: LL CDY. Contributed reagents/materials/analysis tools: AS PDS. Wrote the paper: LL JSB CDY. PLOS ONE | www.plosone.org 8 September 2012 | Volume 7 | Issue 9 | e46301