IL-17 and Synoviocyte Survival Figure 2. Effect of IL-17 on RA FLS apoptosis. RA FLS were treated with SNP 0.01-1 mM for 24 h and apoptosis measured by SS DNA apoptosis kit (A). The results are presented as the fold induction in apoptosis relative to control samples, n = 3, mean 6 SEM of duplicate experiments from 3 different RA donors. * P,0.05 compared to no treatment control. RA FLS were treated with SNP 0.01–1 mM for 24 h (B, left panel) or 0.1 mM over 24 h (B, right panel) and synoviolin expression measured by real-time RT-PCR. The results are expressed as the ratio of synoviolin/b-actin mRNA amplification, n = 3, mean 6 SEM of duplicate experiments from 3 different RA donors. * P,0.05 compared to no treatment control. RA FLS were PLoS ONE | www.plosone.org 5 October 2010 | Volume 5 | Issue 10 | e13416