P2Y12 in Atherothrombosis Figure 2. Inhibition of murine P2Y12 receptors diminishes thrombus formation and provokes platelet disaggregation under flow. Apoe2/2 mice were infused with vehicle solution, ticagrelor or cangrelor (see Figure 1). (A–C) PPACK/heparin anticoagulated blood from treated mice was perfused over native type I collagen at a shear rate of 1000 s21 for 4 min. (A) Representative phase-contrast images after 4 min perfusion (bar = 20 mm). (B) Quantitative effect of ticagrelor on surface area coverage by platelets. (C) Average number of disaggregation events measured from a preformed aggregate during flow. (D) Flow-cytometric evaluation in whole blood of platelets with active aIIbb3 (JON/A mAb) by activation with ADP (20 mM), and of platelets exposing P-selectin (anti-CD62 mAb) by activation with convulxin (Cvx, 10 ng/ml). Bars give percentages of positive platelets. Data are means 6 SE (n = 3–4), *p,0.05. doi:10.1371/journal.pone.0010130.g002 PLoS ONE | www.plosone.org 6 April 2010 | Volume 5 | Issue 4 | e10130