MMP-2 Promotes avb3 Mediated Tumor Cell Migration Figure 6. MMP-2 enhances the adhesion of melanoma cells mediated by avb3 integrin by cleaving fibronectin. (A) Full-length fibronectin (FN) was incubated with activated MMP-2, and the cleaved fragments (FN + MMP-2+ APMA) were resolved on a 10% Tris-HCl gel and subjected to silver staining. (B) Adhesion assays were performed using human A375 cells incubated in full-length fibronectin (FN)- or MMP-2–cleaved fibronectin (FN+MMP-2+APMA)-coated plates as described in MATERIALS AND METHODS (Cell Adhesion Experiment) at different time points (from 5 PLoS ONE | www.plosone.org 10 July 2012 | Volume 7 | Issue 7 | e41591