P2Y12 in Atherothrombosis Figure 7. Inhibition of P2Y12 receptors affects plaque-induced thrombus formation at high shear rate. Citrate-anticoagulated human blood was supplemented with OG488-fibrinogen (25 mg/ml), recalcified with CaCl2/MgCl2 in the presence of 2 pM tissue factor and perfused over homogenized human plaque material at 1000 s21 for 8 min. Blood was pre-incubated with ticagrelor (20 mM) or cangrelor (10 mM) as indicated. (A) Representative phase-contrast and fluorescence images (bar = 20 mm). (B) Average size of thrombi after perfusion. (C) Mean fluorescence intensity from fibrin(ogen)-binding platelets and thrombi. (D) Histograms of features on surface; the estimated numbers of platelets per feature were 9–24 (white), 24–75 (light gray), 75–400 (dark gray) and .400 (black). (E) Disaggregation events measured per platelet aggregate per min. Data are means 6 SE (n = 3), *p,0.05, #p = 0.06 compared to vehicle. doi:10.1371/journal.pone.0010130.g007 PLoS ONE | www.plosone.org 11 April 2010 | Volume 5 | Issue 4 | e10130