physiological and pathological implications of the interactions between H2S and estrogen in proliferative vascular diseases merit further investigation. Supporting Information Figure S1 Estrogen had no effect on CSE expression and H2S production. A, The expression of CSE was not changed by estrogen treatments. After WT-SMCs were incubated with the indicated concentration of estrogen for 72 h, the cells were collected and subjected to western blotting with anti-CSE antibody. B, Estrogen did not affect H2S production. After WT- SMCs were treated with the indicated concentration of estrogen for 72 h, H2S production rate was measured. All the results were representative of five individual experiments. (DOC) Figure S2 Different activation of MAPK by estrogen between WT-SMCs and CSE-KO-SMCs. Time course of the activation of ERK (A), p38 (B), and JNK (C) induced by estrogen. The graphs represent the optical density of the bands of phospho- MAPK normalized with the expression of total-MAPK. 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