Calcineurin Activation and Hair Development Figure 5. Cyclin G2 is a target molecule of Cn/NFATc2 in follicular keratinocytes. (A) The microarray analysis identified 24 genes that showed down-regulated expression in PHK cells treated with either 11R-VIVIT or CsA; ccng2 (cyclin G2) expression showed the greatest decrease after Cn/NFAT inhibition. (B) Time-dependent changes in cyclin G2 and p21waf/cip1 expression in PHK cells treated with 11R-VIVIT. (C) Quantitative analysis of the changes in expression changes of cyclin G2 and p21waf/cip1 in PHK cells after 11R-VIVIT treatment. n = 5 for each sample group. * P,0.01, { P,0.05. (D) Effect of cyclin G2 overexpression on the proliferation of PHK cells. Cells were infected with recombinant adenoviruses carrying cyclin G2 and lacZ at an MOI of 100. Overexpression of cyclin G2 significantly inhibited cell proliferation compared with LacZ-infected cells (*P,0.01). (E) Expression of cyclin G2 in rat hair follicles at postnatal days 9 (first anagen stage) and 49 (second telogen stage). Arrowheads, differentiated keratinocytes in a distal part of the hair shaft; arrows, proliferating keratinocytes in the proximal hair shaft. DP, dermal papillae; HG, hair germ; SG, sebaceous gland. Scale Bar = 50 mm. doi:10.1371/journal.pone.0017685.g005 PLoS ONE | www.plosone.org 9 March 2011 | Volume 6 | Issue 3 | e17685