ALCAM remove a variety of classical cadherins from adherens junctions in different cell types? Several reports have demonstrated that ALCAM and cadherins are present in the same lipid microdomains [5,54,55]: does ALCAM regulate cadherin recruit- ment to these rafts? New studies focused on identification of both extracellular and intracellular binding partners of ALCAM will be critical to understanding of the mechanisms by which ALCAM regulates adherens junctions, cell motility, and invasive capacity. Supporting Information Figure S1 Silencing of ALCAM in HEK cells results in disrupted ß-catenin junctions. HEK cells were transiently transfected with an shRNA construct confirmed to silence ALCAM (sh2), or a negative control scrambled shRNA (sh0). Both constructs included a GFP marker to track transfected cells (pseudocolored blue). HEK cells with silenced ALCAM expression show reduced ß-catenin localization (green) to cell-cell contacts, as References 1. 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