UEV-1 and Glutamate Receptors Figure 1. UEV-1 regulates GLR-1 Trafficking. The fluorescence of (A, B) GLR-1::GFP (GluR), (C, D) SNB-1::GFP (synaptobrevin), (E, F) LIN-10::GFP (Mint2), and (G, H) UNC-43::GFP (CaMKII) was observed along ventral cord neurites of (A, C, E, G) wild-type or (B, D, F, H) uev-1(od10)-mutant animals. The mean size (I) of fluorescent puncta and accretions (arrows) combined are plotted for adult nematodes of the given genotype (stippled bars for wild type, gray bars for uev-1 mutants) and for the given fluorescent reporter (indicated below the graph). (J) The mean density (number per 10 microns of ventral cord length) of fluorescent puncta is plotted for adults of the given genotype. For each genotype, the values have been normalized to the mean value of the wild-type control. Whereas wild-type animals have small GLR-1::GFP puncta, uev-1 mutants accumulate GLR- 1::GFP in large accretions (arrows); other synaptic proteins appear not to be affected in uev-1 mutants. Bar, 5 microns. Error bars are SEM. N = 20–30 animals for each genotype. *P,0.01, ***P,0.001 by ANOVA with Bonferroni multiple comparison tests (only shown for wild type versus mutant for each reporter). doi:10.1371/journal.pone.0014291.g001 promoter (data not shown). We confirmed this, as well as the fact that UEV-1 acts cell autonomously, by transformation with a construct containing uev-1 coding sequences under the glr-1 promoter (Figure 2B–G). Following our identification of od10 as an allele of uev-1, a new deletion allele, ok2610, became available from the C. elegans Genome Consortium. To determine the PLoS ONE | www.plosone.org 3 December 2010 | Volume 5 | Issue 12 | e14291