YY1 Overexpression Negatively Impacts B Cells Figure 1. Cells expressing high levels of YY1 are depleted in B cell populations. Lethally irradiated C57BL/6 mice were reconstituted with 5- FU-treated C57BL/6 donor bone marrow cells transduced with MigR1-FlagYY1 or MigR1 vector alone. Recipient mice were subject to analysis at 10 weeks, 14 weeks, 18 weeks or 26 weeks post transplantation. (A) The top panel shows the cell gating strategy. Bone marrow cells were evaluated for the presence of pro-B (B220+CD43+CD19+AA4.1+IgM2), pre-B (B220+CD432 IgM2AA4.1+), immature B (B220+CD432IgM+AA4.1+), and re-circulating B (B220+CD432IgM + AA4.12) cells. GFP+ percentages are listed in each B cell compartment in MigR1 or MigR1-FlagYY1 reconstituted mice 10 weeks (left PLoS ONE | www.plosone.org 3 January 2012 | Volume 7 | Issue 1 | e30656