Hippocampal Neurogenesis Figure 8. The expression pattern of markers for cell maturation during rodent hippocampal neurogenesis is conserved during human hippocampal neurogenesis. (A–E5) Indirect immunohistochemistries performed on human hippocampal coronal sections of 58-years-old individual. (A) DAB immunohistochemistry for the neuronal marker NeuN shows the neuro-anatomical structure of the hippocampus. (B1–B4) Mature granule neurons and astrocytes/radial glia-like cells express NeuN (green) and GFAP (red), respectively. (C1–C4) Neurons located in the DG express the granule neuron identity marker Prox1. (D1–D5) Pax6 and NeuN expression do not co-localize, suggesting the existence of astrocytes/radial glial stem cell in human hippocampus. (E1–E5) The presence of Sox2+ cells in the SVZ of the DG suggests the occurrence of ongoing neurogenesis. (F) The existence of radial glia stem cells in the human hippocampus is identified by RT-PCR for the markers Pax6, GFAP and GLAST. The molecular bibliography of cellular markers and transcription factors expressed during hippocampal neurogenesis in rodents is conserved during hippocampal neurogenesis in human. Rectangular images on the bottom and right of the panels B4, D4 and D5, E4 and E5 represent a projection of 14-Z stacks PLoS ONE | www.plosone.org 13 March 2009 | Volume 4 | Issue 3 | e4779