Myelin Induces Inflammation Figure 6. Effect of myelin on NF-kB activation and cytokine expression in bone marrow-derived macrophages from CR3 KO mice. (A) Macrophages from CR3 KO mice were treated with myelin for the indicated time and phosphorylation/degradation of IkB-a were assessed by western blot. (B) Macrophages from CR3 KO mice were treated with myelin for 6 and 12 hours. Cytokine and chemokine expression in cells at mRNA levels were detected by qRT-PCR. (C) Macrophages from WT and CR3 KO mice were treated with myelin for 24 hours and cytokines in the supernatants of cell culture were detected by ELISA. Phosphorylation levels were normalized to non-phosphorylated total proteins. Data were expressed as mean 6 SEM and similar results were obtained from three independent experiments. (n = 3, *P,0.05 vs. CR3 KO + myelin; t-test). doi:10.1371/journal.pone.0009380.g006 PLoS ONE | www.plosone.org 9 February 2010 | Volume 5 | Issue 2 | e9380