IL-36a and Pulmonary Inflammation Figure 4. Intratracheal instillation of IL-36a induced neutrophil influx in the lungs of IL-1ab2/2 mice. A) Total cell counts from BAL fluid recovered from mice 24 h following a single i.t. instillation of PBS or 10 mg IL-36a. B) Differential cell count percentages and C) Differential cell count numbers in the BAL fluid recovered from mice 24 h following a single i.t. instillation of PBS or 10 mg IL-36a. *Indicates significant differences (P,0.05) compared to PBS treated mice. Data represent mean6SEM from 3–4 mice/group. D) Flow cytometry on cells recovered from BAL fluid from mice 24 h following a single i.t. instillation of PBS or 10 mg IL-36a. A majority of cells from the IL-36a instilled lungs were CD11c2CD11b+Ly6G+ neutrophils. Depicted flow cytometry plots are representative of 3–4 mice/group. H) Hematoxylin & Eosin stained lung sections isolated from mice 24 h following a single i.t instillation of PBS or 10 mg IL-36a. Depicted sections are representative of 3–4 mice/group. doi:10.1371/journal.pone.0045784.g004 PLOS ONE | www.plosone.org 8 September 2012 | Volume 7 | Issue 9 | e45784