inhibitors. The physiological significance of butylene interrupted PUFA in cancer cells calls for further detailed investigation. Our present results provide an impetus to better understanding the role of fatty acid desaturases, especially FADS2 as a tumor suppressor in neoplastic disorders. Materials and Methods Plasmid vector construction The protein coding sequences of FADS1 (GenBank Accession# EF531577) and FADS2 (GenBank Accession# EU780003) were cloned into pcDNA3.1 expression vector using cDNA from neonate baboon liver tissue. The cDNA was obtained from banked samples drawn from a study approved by the Cornell University Institutional Animal Care and Use Committee (IACUC, protocol # 02–105). Analysis and comparison of amino acid sequence of baboon FADS1 showed 95% identities and 97% positives with human FADS1 (AF084558), whereas, baboon FADS2 showed 98% identities and 99% positives with human FADS2 (NM_004265). Earlier, we have shown novel D8- desaturation function using baboon FADS2 [14]. MCF7 cell culture and fatty acid supplementation For transfection experiments MCF7 cells were grown in MEM- a media with 10% Lipid-reduced FBS (media and serum obtained from HyClone) at 37uC in a humidified environment with 5% CO2. Human breast cancer MCF7 cells (American Type Culture References 1. Chandrasekharappa SC, Guru SC, Manickam P, Olufemi SE, Collins FS, et al. (1997) Positional cloning of the gene for multiple endocrine neoplasia-type 1. Science 276: 404–407. 2. Chung CC, Ciampa J, Yeager M, Jacobs KB, Berndt SI, et al. (2011) Fine mapping of a region of chromosome 11q13 reveals multiple independent loci associated with risk of prostate cancer. Hum Mol Genet;doi: 10.1093/hmg/ ddr189. 3. Koreth J, Bakkenist CJ, McGee JO (1999) Chromosomes, 11Q and cancer: a review. J Pathol 187: 28–38. 4. Rosa-Rosa JM, Pita G, Gonzalez-Neira A, Milne RL, Fernandez V, et al. 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Anal Chem 71: 1981–1989. PLoS ONE | www.plosone.org 4 November 2011 | Volume 6 | Issue 11 | e28186