Ets1 in Squamous Neoplasia Figure 6. Ets1 expression induces angiogenesis. (A) Macroscopic appearance of the dermal surface of the dorsal skin of wild type and BT mice. (B) Hematoxylin and eosin stained sections of dorsal skin from wild type and BT mice. Note the presence of increased numbers of blood vessels in the dermal compartment of the BT sample (black arrowheads). (C) PECAM-1 (green) staining of skin samples from wild type and BT skin. Sections were stained with antibodies to keratin 14 (K14, red) to reveal epidermal keratinocytes. doi:10.1371/journal.pone.0004179.g006 Figure 7. The Ets1 induced phenotype is reversible and age-dependent. (A) Phenotype of BT and control mice at the end of a 3–6 week induction period and again after 6 weeks of transgene suppression on a Dox diet. Note that BT mice appear completely normal after six weeks of rescue. (B) Skin samples taken from BT and control mice at the height of the Ets1 induced phenotype and again after transgene suppression. (C) Comparison of the phenotype of BT mice that were induced for the first time at a young age (early induction) or a late age (late induction). Note the more severe phenotype evident in the early induction. doi:10.1371/journal.pone.0004179.g007 PLoS ONE | www.plosone.org 7 January 2009 | Volume 4 | Issue 1 | e4179