LRP1 Controls Vascular PI3K Figure 4. Disorganization of the actin cytoskeleton in LRP12/2 SMCs is prevented by blocking PI3K activation by PDGFRb. (A) Primary SMCs generated from mouse aortas. (a) Phase contrast image. Scale bar, 80 mm. (b) Immunofluorescence using anti-smooth muscle actin monoclonal antibody (red). Blue: DAPI staining of nuclear DNA. Scale bar, 40 mm. (c, d) Detection of LRP1 (green) by immunofluorescence by a rabbit anti-LRP1 polyclonal antibody. Blue: DAPI staining. WT, wild type. Scale bars, 40 mm. (e) Immunoblotting was performed to verify the presence or absence of LRP1 protein in the wild type and LRP12/2 SMCs using the same polyclonal anti-LRP1 antibody. (B) Immunofluorescence of smooth muscle actin (red) in primary SMCs. Actin disorganization in LRP12/2 and LRP12/2; LDLR2/2 SMCs. Normal organization of the actin cytoskeleton is restored in primary PDGFRb F2/F2 SMCs. Blue: DAPI. Scale bar: 20 mm. doi:10.1371/journal.pone.0006922.g004 PLoS ONE | www.plosone.org 6 September 2009 | Volume 4 | Issue 9 | e6922