MiR-338-3p on HBx Deletion Mutants (HBx-d382) Figure 1. HBx-d382 enhances the non-anchored growing ability of transfected hepatocytes. (A–B) The identification of stable HBx transfection in LO2 cells. (A) The HBx gene was identified by RT-PCR. (B) Western blotting showed the expression of HBx in LO2 cells. 1: LO2; 2: LO2/ pcDNA3.0; 3: LO2/HBx-d382; 4: LO2/HBx; M: marker. (C) Soft agar colony formation assay of transfected LO2 cells. The rate of colony formation in HBx- PLOS ONE | www.plosone.org 3 August 2012 | Volume 7 | Issue 8 | e43204