Table 1. High salinity-induced changes in metabolite levels. TAG MASS REV MATCH COMPOUND TAG RI 1740.00 1421.52 1804.48 1259.77 1860.48 1348.79 2961.58 1978.80 1983.47 1877.02 2303.56 1528.27 1766.29 1338.96 1301.91 1887.43 2668.52 1477.67 2715.89 1914.96 2078.23 1555.80 1262.14 1179.06 1521.02 3344.18 1253.80 1792.80 2245.96 2192.32 1310.30 2620.12 1545.59 1373.34 1290.44 2722.90 1931.81 2091.59 1209.27 1524.11 272 118 147 156 129 147 117 129 129 229 299 158 100 147 147 229 129 117 160 147 129 146 135 113 156 204 147 204 338 116 147 129 147 101 130 143 218 157 144 100 917 975 980 958 978 974 965 950 948 970 916 944 946 956 970 806 912 987 973 960 966 955 999 999 981 936 982 894 926 950 978 970 967 968 982 994 999 970 968 927 Aconitic acid Ascorbic acid Ascorbic acid total Aspartic acid Citric acid Dehydroascorbic acid Ethanolamine Fructose Fumaric acid Galactinol Galactonic acid Gluconic acid Glucose Glucose-6-phosphate Glutamic acid Glutamine Glyceric acid Glycine Indole-3-acetonitrile Lactose Malic acid Maltose Mannitol Myo-inositol Phenylalanine Phosphoric acid Proline Pyroglutamic acid Raffinose Serine Shikimic acid Sinapic acid. trans Spermidine Starch Succinic acid Sucrose Threonic acid Threonic acid-1.4-lactone Threonine Trehalose Tyrosine Uric acid Valine c-Aminobutyric acid ctrl. 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 6h 1.06 0.73 1.08 0.87 1.96 2.21 1.42 0.72 1.80 1.28 0.96 1.74 0.74 0.71 1.20 2.14 1.00 0.45 1.79 1.08 1.98 0.39 0.88 0.91 1.39 0.78 1.94 1.11 1.85 1.08 0.92 1.43 0.79 0.78 1.35 1.15 1.51 1.12 0.90 0.73 4.30 1.03 1.64 1.46 12h 0.96 0.72 0.91 0.85 1.46 1.57 1.02 0.64 1.59 1.49 0.92 1.87 0.93 0.50 2.19 0.32 0.99 0.68 2.02 0.88 1.74 0.57 1.05 0.95 2.20 0.67 1.85 0.69 1.96 1.92 0.81 1.09 0.82 0.83 1.22 1.21 1.54 1.07 1.14 3.36 2.37 1.07 1.32 1.23 1d 1.41 0.69 0.89 0.87 1.57 1.54 0.90 0.47 1.26 0.97 0.80 1.25 0.79 0.68 2.32 1.34 0.96 0.51 1.33 0.83 1.52 0.55 1.05 0.94 1.61 0.52 1.88 0.90 2.35 2.47 0.73 0.95 0.39 0.62 1.16 1.17 1.32 1.15 1.15 1.09 4.56 1.04 1.32 1.05 Metabolic Stress Adaptation 3d 0.56 0.60 0.82 1.18 1.11 1.54 0.99 1.82 1.08 7.35 0.75 2.29 1.39 0.69 4.20 1.14 0.78 2.80 1.63 1.09 1.17 12.04 1.00 1.21 4.37 0.69 5.41 1.08 26.79 5.97 0.78 1.29 0.32 0.55 1.15 0.95 0.95 0.74 1.54 1.47 7.20 1.10 4.98 2.47 5d 0.37 0.36 0.86 0.68 0.84 2.52 1.42 2.85 0.93 27.54 0.79 5.90 1.54 0.41 2.49 2.96 0.82 8.08 1.38 0.76 0.96 11.41 0.91 1.79 6.78 0.48 22.10 0.92 136.14 4.76 0.49 1.04 0.23 0.31 1.42 1.03 1.01 0.71 1.37 1.51 9.39 1.28 5.16 19.81 The metabolite contents of rosette leaves of 4 week old, soil-grown Arabidopsis thaliana were determined upon watering with 150 mM NaCl. Pools of ten individual leaf rosettes were analysed four times using GC-MS. Compounds were identified by their retention index (TAG RI), quantitative mass tag (TAG MASS) and reverse match value (REV MATCH) of the mass spectra comparison. Ascorbic acid and dehydroascorbic acid levels were determined by HPLC and starch levels were quantified spectrophotometrically. Values are x-fold changes compared to the corresponding unstressed controls. Bold letters indicate significant changes of metabolites (t-test; P- value ,0.05). doi:10.1371/journal.pone.0003935.t001 PLoS ONE | www.plosone.org 3 December 2008 | Volume 3 | Issue 12 | e3935