Myelin Induces Inflammation Figure 2. Inflammatory effect of myelin in spinal cord from WT and CR3 KO mice. (A) Neutrophils were positive for Ly-6G (green) but negative for IBA-1 (red) detected by double immunostaining. (B) Neutrophil and macrophage infiltration in spinal cord at 1 d after PBS injection detected by double immunostaining. (C) Neutrophil infiltration (Ly-6G+, green) in spinal cord at 1 d after myelin injection, along with injection region (solid line), marginal region (dashed line) and enlarged injection region. (D) The number of neutrophils at injection region and marginal region in spinal cord at 1 d after myelin or PBS injection. (E) Macrophage infiltration (IBA-1+, red) in spinal cord at 4 d after myelin injection, along with injection region (solid line), marginal region (dashed line) and enlarged injection region. (F) The number of macrophages at injection and marginal region in spinal cord at 4 d after myelin or PBS injection. Scale bar = 200 mm. Data were expressed as mean 6 SEM. (n = 4 at each time point, *P,0.05 vs. WT + PBS; #P,0.05 vs. CR3 KO + myelin; t-test). doi:10.1371/journal.pone.0009380.g002 PLoS ONE | www.plosone.org 5 February 2010 | Volume 5 | Issue 2 | e9380