Imprinted miRNA Signature in Male GS Cells Figure 1. Genomic location and expression of imprinted miRNAs analyzed in this study. A: Genomic structure of Gnas-Nespas (i), Dlk1- Dio3 (ii) and Igf2-H19 (iii) imprinted gene clusters analyzed in this study. Maternally expressed genes and miRNAs are shown in red while paternally expressed genes and miRNAs are shown in blue. Arrow shows the direction of transcription. Lollipops represent the position of imprinting control region (ICR) or differentially methylated regions (DMR) of the respective gene cluster. Red lollipop indicates methylation in maternal chromosome while blue lollipops indicated methylation in paternal chromosomes. Not drawn to scale. B: Expression of miR-296-3p, miR-296-5p, miR-127, miR-127- 5p and miR-483 imprinted miRNAs in mouse somatic cells (open box), sperm (crossed box) and testis-tissue (closed box). Values above the bars indicate relative abundance of miRNAs normalized to the expression of respective miRNA in somatic cells. Different alphabet (a, b, c) on the bars indicate statistical difference (P,0.01) in gene expression. doi:10.1371/journal.pone.0022481.g001 PLoS ONE | www.plosone.org 3 July 2011 | Volume 6 | Issue 7 | e22481