Integrin-Ligand Interactions Control Migration Figure 9. Effect of myosin II activation and Mn2+ on HL-60 cell migration. (A) Immunoblotting for pRLC shows that cells migrating on FN or ICAM-1 show similar levels. For the HL-60 cells, pRLC levels appear to be dose independent on both substrates. Representative blots are shown, and relative-fold increase of pRLC presented. The data are derived from at least three experiments for each substrate. (B) RLC activation does not affect the migratory properties of HL-60 cells on FN. HL-60 cells were doubly transfected with paxillin-mCherry and RLC, RLC-A,D, or RLC-D,D with GFP. Experimental time: 4 minutes. Scale Bar = 10 mm. (C) Migration of HL-60 cells was inhibited by Mn2+. Three individual cells before and after Mn2+ treatment were plotted. Set 1: red; set 2: green; set 3: blue. P value = 0.012. doi:10.1371/journal.pone.0040202.g009 PLoS ONE | www.plosone.org 12 July 2012 | Volume 7 | Issue 7 | e40202