PINK1 and Drp1 Figure 5. Mitochondrial effects due to loss of PINK1 can be rescued by knockdown of Drp1. (A) Exposure of control shRNA or PINK1 shRNA stable cell lines to Drp1 siRNA for four days results in an approximately 70% knockdown of Drp1 (arrow) compared to a non-specific GFP siRNA. b-actin shows equal loading. Molecular weight markers are in kilodaltons. (B) Live cell images of mito-YFP transfected control shRNA (upper panels) and PINK1 shRNA (lower panel) cells exposed to a non-specific siRNA against GFP (left panels) or an siRNA against Drp1 (right panels). Scale bar is 2 mm, applies to all fluorescence micrographs. (C) FRAP curves show that under basal situations, there was a difference between the control shRNA line and the PINK1 shRNA line. These differences were partially normalized after the cells were exposed to an siRNA against Drp1. Each point is PLoS ONE | www.plosone.org 9 May 2009 | Volume 4 | Issue 5 | e5701