condition of the cells on these days is also reflected in the reduced levels of a-tubulin seen on the corresponding Western blots, despite loading equal mg of total protein from each sample. B. MCF7 cells were infected with the same freshly prepared, high titer virus expressing non-target shRNA or shEcad#21, #22, or #23. CDH1 mRNA levels in these cells were quantified using qPCR at days 2, 4, 7, and 15 post infection (top); E-cadherin protein expression was determined at days 4, 7, and 15 p.i. using Western blot (bottom). Found at: doi:10.1371/journal.pone.0013665.s003 (0.97 MB TIF) References 1. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK (2007) WHO Classification of tumours of the central nervous system; Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, eds. Lyon: IARC. 2. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, et al. (2007) The 2007 WHO classification of tumours of the central nervous system.[erratum appears in Acta Neuropathol. 2007 Nov;114(5):547]. 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