Regulation of Dark and Photoconversion of phyB-401 Figure 6. RG9 treatment is ineffective in depleting cR-induced PHYB-401:YFP NBs in darkness. Seedlings expressing PHYB-401:YFP or PHYB:YFP were grown for 3 days in darkness and on day 4 they were irradiated with cR (3 mmol m22 s21). After the 24 h R irradiation the seedlings were transferred to darkness (2RG9) or were treated with a RG9 pulse (40 mmol m22 s21) prior to transfer to darkness (+RG9). Subcellular localization of the PHYB-401:YFP and the PHYB:YFP fusion proteins were monitored after the light treatments. Sampling time (h) after the light treatments is indicated in the left panels. Scale bar is shown. doi:10.1371/journal.pone.0027250.g006 PLoS ONE | www.plosone.org 6 November 2011 | Volume 6 | Issue 11 | e27250