Supporting Information Figure S1 Akt and GSKb phosphorylation by DHIV-GFP in CHME5cells. 16105 CHME5 cells were transduced by DHIV- GFP as described in Figure 2, and the cell lysates were analyzed by western blots for total (t) and phospho (p) specific Akt and GSK3b. The fold differences in the signals are marked. Found at: doi:10.1371/journal.pone.0013121.s001 (1.56 MB TIF) Figure S2 Akt and GSKb phosphorylation by Tat expression in CHME5 cells. Lysates from 16105 CHME5 sublines with References 1. Aquaro S, Bagnarelli P, Guenci T, De Luca A, Clementi M, et al. (2002) Long- term survival and virus production in human primary macrophages infected by human immunodeficiency virus. J Med Virol 68: 479–488. 2. Cosenza MA, Zhao ML, Lee SC (2004) HIV-1 expression protects macrophages and microglia from apoptotic death. Neuropathol Appl Neurobiol 30: 478–490. 3. Mattson MP, Haughey NJ, Nath A (2005) Cell death in HIV dementia. Cell Death Differ 12 Suppl 1: 893–904. 4. 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Vink SR, Schellens JH, van Blitterswijk WJ, Verheij M (2005) Tumor and normal tissue pharmacokinetics of perifosine, an oral anti-cancer alkylpho- spholipid. Invest New Drugs 23: 279–286. PLoS ONE | www.plosone.org 8 September 2010 | Volume 5 | Issue 9 | e13121