Conditional Transneuronal Tracing with PRV Figure 3. Neuroinvasive Profiles of PRV Recombinants. The number of infected neurons in 24 coronal planes sampling the neuraxis 5 days following injection of PRV 267 into right kidney (solid line) and PRV-263 into the left kidney (dashed line) is illustrated. Cases are arranged according to the magnitude of viral invasion. Case numbers are indicated in the upper left of each graph and the post inoculation survival interval is listed below each case number. The location of the planes of section sampled for each case is illustrated in the sagittal schematic included in the upper right of Case 4. The X-axis of each graph indicates the position of the 24 coronal planes relative to Bregma (b), an anatomical marker of the confluence of bone sutures on the rostral skull. The Y-axis indicates the number of infected neurons. Although the magnitude of infection varied between cases, the number of infected neurons on the left and right side of the brain was comparable for each coronal plane in each case. This finding is consistent with the conclusion that each recombinant invaded preautonomic circuitry from the kidney at equivalent rates in each experimental animal. The schematic diagram is adapted from the atlas Brain Maps: Structure of the Rat Brain [28]. doi:10.1371/journal.pone.0021141.g003 PLoS ONE | www.plosone.org 6 June 2011 | Volume 6 | Issue 6 | e21141