Novel CCR2-RFP Mice in EAE Figure 3. Analysis of peritoneal exudate cells from Ccr2-RFP Cx3cr1-GFP mice in a sterile peritonitis model. WT, Ccr2RFP/+Cx3cr1GFP/+, Ccr2RFP/+Cxc3r1GFP/GFP, Ccr2RFP/RFPCx3cr1GFP/+, and Ccr2RFP/RFPCx3cr1GFP/GFP mice were injected intraperitoneally with thioglycollate, and inflammatory exudate cells were harvested 24 h later. Cells were stained with F4/80. (A) RFP/GFP profiles of F4/80+ peritoneal cells from naive mice (top panels) and 24 hr after injection (bottom panels). (B) Percentage of exudate cells staining positive for F4/80. Values are mean 6 SEM. n = 4 mice/group. Results are representative of two similar experiments. doi:10.1371/journal.pone.0013693.g003 each genotype (Figure 4C). CD45hi leukocytes accounted for 36.263.5% of total cells in Ccr2RFP/+Cx3cr1GFP/+ mice and 35.263.5% in Ccr2RFP/RFPCx3cr1GFP/+ mice (P.0.5). To further characterize the inflammatory infiltrate, we stained samples with antibodies against CD115 and Ly6C. Cells were gated by CD45 expression; CD45hi cells were then analyzed for PLoS ONE | www.plosone.org 5 October 2010 | Volume 5 | Issue 10 | e13693