ISH Profiling of Rat Cortex Figure 6. Spatial distributions of RORbeta, ER81 and Nurr1 in the standardized cortical map. (A) Average standardized layer maps (layers 2/3, 4, 5 and 6) for in situ hybridization immunohistochemistry (ISH) of RORbeta, ER81 and Nurr1. Black lines indicate the cytoarchitectonic borders of the cortical area defined in Fig. 5A. (B) Coefficient of variance (CV) of standardized layer maps (layers 2/3, 4, 5 and 6) for in situ hybridization immunohistochemistry (ISH) of RORbeta, ER81 and Nurr1. White color represents the CV values of the pixels with low average values (see Materials and Methods). doi:10.1371/journal.pone.0003266.g006 PLoS ONE | www.plosone.org 7 September 2008 | Volume 3 | Issue 9 | e3266