P2Y12 in Atherothrombosis Figure 6. Inhibition of P2Y12 receptors reduces aggregate size at high but not low shear in cone and plate(let) analyzer. Citrate-anticoagulated human blood was preincubated with vehicle, ticagrelor (20 mM) or cangrelor (10 mM) for 10 min. Blood samples were subjected to a shear rate of 500 or 5000 s21 for 2 min in a cone and plate(let) analyzer (CPA). (A) Representative images of platelet 2 aggregates formed on the surface after 2 min (bar = 20 mm). (B) Average aggregate size in mm . Data are means 6 SE (n = 4–6), *p,0.05 compared to vehicle. doi:10.1371/journal.pone.0010130.g006 plaque material in vitro. Furthermore, this P2Y12 de- pendency of thrombus stability is maintained in the presence of coagulation, but most pronounced at high shear flow conditions. Acknowledgments We thank dr. R. Megens and Laura Jackman for participation in experiments. We also thank Schering Plough Research Institute for providing the P2Y12 deficient mice. PLoS ONE | www.plosone.org 10 April 2010 | Volume 5 | Issue 4 | e10130