miR-150 Targets Cxcr4 to Regulate MNC Mobilization Figure 3. miR-150 targets Cxcr4 and regulates MNC migration. (A) A putative target site of miR-150 highly conserved in the Cxcr4 mRNA 39- UTR as predicted by computational analysis. (B) miR-150 expression was decreased in BM-MNCs after AMI as validated by real-time PCR. (C) CXCR4 protein expression in MNCs significantly increased by transfection with miR-150 inhibitor. (D) BM-MNCs isolated from mice with LAD ligation enhanced migration capacity of BM-MNCs in response to SDF-1a as evaluated by transwell migration system. Transfection of wild type MNCs with anti-miR-150 also increased the number of migrating cells. doi:10.1371/journal.pone.0023114.g003 PLoS ONE | www.plosone.org 6 October 2011 | Volume 6 | Issue 10 | e23114