microarray screening. A) mean value of methylation for each CpG analysed. B) CpG methylation in each samples. A cut-off value was set at 20%, meaning that value above 20% is considered as hypermethylated, whereas above means hypomethylated. Position of each CpG is indicated (TSS = +1). In red is indicated the Cpg analysed on Illumina chip. White circle, hypomethylated CpG; black circle, hypermethylated CpG. (TIF) Figure S4 Correlation between UTF1 promoter mean CpG methylation and UTF1 mRNA expression. The mean of CpG methylation was calculated for each sample. Data from qPCR were log transformed before being plotted. Correlation between UTF1 mean CpG methylation and mRNA expression was evaluated by Spearman’s rank correlation test, reported p- value was two-sided. (TIF) Figure S5 Correlation between UTF1 promoter mean CpG methylation and UTF1 mRNA expression in cancer cell lines treated or not with 5-AZA. The mean of CpG methylation was calculated for each sample. Correlation between UTF1 mean CpG methylation and mRNA expression was evaluated by Spearman’s rank correlation test, reported p-value was two-sided. (TIF) Figure S6 UTF1 promoter methylation analysis by direct bisulfite pyrosequencing in NCCIT and NCCIT differentiated with retinoic acid (NCCIT RA). Position of References 1. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, et al. (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. International journal of cancer Journal international du cancer 127: 2893–2917. 2. zur Hausen H (2009) Papillomaviruses in the causation of human cancers - a brief historical account. Virology 384: 260–265. 3. Hall S, Lorincz A, Shah F, Sherman ME, Abbas F, et al. (1996) Human papillomavirus DNA detection in cervical specimens by hybrid capture: correlation with cytologic and histologic diagnoses of squamous intraepithelial lesions of the cervix. Gynecologic oncology 62: 353–359. 4. 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