PINK1 and Drp1 Figure 2. PINK1 influences mitochondrial morphology in response to rotenone. (A) M17 cell lines were stained with mitotracker and cells assigned to one of three morphological categories; intact, truncated or fragmented. Scale bar in the bottom panel is 10 mm, applies to all fluorescence micrographs. (B, C) Blinded counts of 45–130 cells in each of three experiments were performed in cell lines overexpressing PINK1 (B) or lacking PINK1 (C) with or without exposure to 100 nM rotenone for 24 hours. Cells with truncated or fragmented mitochondria are expressed as a percentage of the total counted. Error bars indicate the SEM between experiments. Statistical significance was assessed by two-way ANOVA for mitochondrial morphology and cell line. P values for cell groups were significant and are shown above each figure. (D) Transmission electron PLoS ONE | www.plosone.org 5 May 2009 | Volume 4 | Issue 5 | e5701