Regulation of T Cell Priming by Lymphoid Stroma Figure 2. Regulation of T cell activation by LN Stromal cells via an IFNc mediated and proximity-dependent mechanism. (a–d) CD8+ T cell proliferation under various stimuli. Cultures were incubated for 48 hours prior to analysis by flow cytometry. (a) CFSE dilution profiles of OTI T cells cultured with FRC.5 cells as well as either SL8 peptide-pulsed BMDCs (BMDC+SL8), plate-bound anti-CD3 (CD3), plate-bound anti-CD3 and soluble anti-CD28 (aCD3/aCD28), or soluble PMA and Ionomycin. (b–d) The percentage of blasting OTI cells are represented as the percentage of cells progressing past the first, non-proliferative, CFSE peak (% of Blasts). Cells were activated with various concentrations of FRC.5 under the following conditions: (b) activated with 104 peptide-loaded BMDCs (c) plate-bound anti-CD3, plate-bound anti-CD3 and soluble anti-CD28, or soluble PLoS ONE | www.plosone.org 4 November 2011 | Volume 6 | Issue 11 | e26138