Lysine Acetylation and Foxp3 Function Figure 2. Single Foxp3 lysine mutations affect Treg suppressive function and gene expression. (A) CD4+ CD252 T cells transduced with retroviruses encoding WT Foxp3, K16R, K17R, K18R, K19R or EV; Foxp3 staining showed .80% transduction efficiency. (B) Effects of single lysine mutations on Treg suppressive activity. (C) RNA derived from CD4+CD252 T cells transduced with WT Foxp3, K17R, K18R or EV were analyzed for CTLA4 (in the absence of CD3/CD28 mAbs) and IL-2, IL-4, IL-17 and IL-21 (in the presence of CD3/CD28 mAbs) gene expression by qPCR. Data were normalized to 18S; *p,0.05, **p,0.01 compared to WT Foxp3. Graphs show means 6 SD and results are representative of 3 independent experiments. doi:10.1371/journal.pone.0029035.g002 PLoS ONE | www.plosone.org 4 January 2012 | Volume 7 | Issue 1 | e29035