VASH1 Enhances Stress Resistance of Endothelium Figure 7. VASH1 protects mice from death with acute lung injury induced by paraquat treatment. (A) Paraquat was administered to WT (N = 8, dotted line) or VASH1 (+/2) mice (N = 8, solid line), and the survival was observed over 10 days. Kaplan-Meier survival analysis showed significant difference. (B) Two days after paraquat administion, histological analyses of the lungs were performed. H&E staining is shown on the left, and immunostaining for 8-OHdG on the right. (C) Two days after paraquat administration, total protein and number of cells in the BALF were determined. *Significant difference (N = 7). (D) Paraquat was administered to VASH1 (+/2) mice intratracheally infected with AdVASH1 (N = 10, dotted line) or AdLacZ (N = 11, solid line), and the survival was observed over 10 days. Kaplan-Meier survival analysis showed significant difference. (E) Two days after paraquat administion to AdLacZ or AdVASH1 mice, histological analyses of lungs were performed. H&E staining is shown on the left; and immunostaining fo 8-OHdG, on the right. (F) Two days after paraquat administration, BALF was collected; and total protein and number of cells in the BALF were determined. *Significant difference (N = 10). (G) Three days after the intratracheal infection of mice with AdVASH1 or AdLacZ, their lungs were removed and tissue extracts prepared. Western blotting for VASH1, SOD2, and SIRT1 in the extracts was performed. doi:10.1371/journal.pone.0046459.g007 PLOS ONE | www.plosone.org 9 October 2012 | Volume 7 | Issue 10 | e46459