VIG and Vig2 Impact Chromatin Figure 3. Vig and vig2 mutants are dominant modifiers of PEV. (A, B) VIG and Vig2 production is disrupted in mutant lines as shown by Western analysis. VigEP812 is a P-element insertion in the coding region of vig; vig2PL470 is a piggyBac-based insertion in vig2. Protein extracts from adult flies homozygous for these mutations were used as starting material. (C) Examination of the yellow variegating reporters B79, J545, and J448 in a vig or vig2 haplo deficient background. Bar graphs represent the number of pigmented patches on males’ dissected abdomens normalized to the control (set at 1). Error bars indicate standard errors. Typical images of the observed phenotypes are shown above the graph. doi:10.1371/journal.pone.0006182.g003 PLoS ONE | www.plosone.org 5 July 2009 | Volume 4 | Issue 7 | e6182