cyp6a17 Is Required for TPB in Drosophila Figure 1. Genes differentially expressed by altered PKA activity in the mushroom bodies. (A) CLUSTER image of 130 PKA-regulated genes. Each column represents changes in the transcript level of candidate genes. Red indicates up-regulation by PKACA, and green represents down- regulation by PKADN. Identities of all PKA-regulated genes are provided on the right side of the CLUSTER image, placed in the same order as their relative position in the CLUSTER image. (B) Van diagram for 103 candidate genes. Blue area: 44 transcripts responded to PKADN but not PKACA. Red area: 39 transcripts responded to PKACA but not PKADN. Blue and red overlap area: 20 transcripts showed changes by both PKADN and PKACA. (C) Transcript profiles of cyp6a17 by PKADN or PKACA. (D) RT-PCR confirmed the results of genome-scale microarray experiment. The relative mRNA transcript level was measured (histograms) by real-time qPCR analysis. gapdh1 was used as internal control. The number of tests: N = 4. Two asterisks, P,0.001. doi:10.1371/journal.pone.0029800.g001 PLoS ONE | www.plosone.org 3 December 2011 | Volume 6 | Issue 12 | e29800