CDC25A-Q110del Novel Isoform Role in Lung Cancer Figure 2. Real time-PCR quantification of CDC25AQ110del in HBEC and NSCLC cell lines. A. Real-time PCR assay to assess the quantity of CDC25AQ110del relative to the total CDC25A transcripts in NSCLC cell lines and tissue samples, the ‘‘Total’’ real time-PCR assay determines total CDC25A template in reaction (Cttot) while the ‘‘wt’’ real time-PCR assay determines the target gene which is the CDC25Awt template (Ctwt), then PLOS ONE | www.plosone.org 5 October 2012 | Volume 7 | Issue 10 | e46464