IL-17 and Synoviocyte Survival Figure 3. Effect of IL-17RA or IL-17RC knockdown on apoptosis, synoviolin expression in RA FLS. To confirm specificity of siRNA knockdown of individual IL-17 receptors, RA FLS were nucleofected (amaxa) for 24 h with 0.5 mg IL-17RA (siRA), 0.05 mg IL-17RC (siRC) or siCONTROL siRNA (sictl) serum starved overnight then treated 50 ng/ml IL-17A for 8 h. IL-17RA or IL-17RC protein expression was measured by Western Blot. Membranes were stripped and reprobed for actin as a loading control (A). IL-17RA or IL-17RC mRNA expression was measured by real- PLoS ONE | www.plosone.org 7 October 2010 | Volume 5 | Issue 10 | e13416