Pglyrp3/4 Promote Treg and Inhibit Th17 Responses Figure 8. Pglyrp32/2 mice have high numbers Th17 and low numbers of Treg cells in the affected skin in the oxazolone model of atopic dermatitis. (A) Numbers of CD4+ cells and Th17 cells in the ears or (B–D) percentages of Th1, Th2, Th17, and Treg cells in the ears, cervical lymph nodes, and spleen in sensitized WT and Pglyrp32/2 mice on days 13 or 20 of ear treatment with oxazolone, measured by flow cytometry; means 6 SEM of 5–9 mice/group (*, P,0.05; **, P,0.005; Pglyrp32/2 versus WT) or representative dot plots are shown. (E) Expression of receptors for chemokines that attract Treg cells in cervical lymph nodes of sensitized WT and Pglyrp32/2 mice on day 20 of ear treatment with oxazolone measured by qRT-PCR; amounts of mRNA are shown as means 6 SEM of 3 arrays from 5 mice/group. doi:10.1371/journal.pone.0024961.g008 PLoS ONE | www.plosone.org 10 September 2011 | Volume 6 | Issue 9 | e24961