LRP1 Controls Vascular PI3K Figure 3. Reduced atherosclerotic lesions in smLRP12/2; LDLR2/2; PDGFRb F2/F2 mutant mice. (A) Unopened (a–c) & Oil Red O stained (d– f) aortas from 10-month old mice of the indicated genotypes. Mice were maintained on standard rodent chow diet. Arrows indicate lipid-laden atherosclerotic lesions. (B) Histological analysis of aortas from 8-month old mice of the indicated genotypes. g–l: HE stain, m–r: trichrome stain, s–x: elastin stain. g,h,i,m,n,o,s,t u: aortic arch; j,k,l,p,q,r,v,w,x: thoracic aorta. Scale bar, 20 mm. (C, D, E, F) Atherosclerotic lesions (C), length (D), thickness (E) and cell number per cm2 (F) of the indicated genotypes were quantified using Image J software (NIH). Results from 3 mice per group are presented as mean6SD. * p,0.05, ** p,0.01, *** p,0.001. doi:10.1371/journal.pone.0006922.g003 PLoS ONE | www.plosone.org 5 September 2009 | Volume 4 | Issue 9 | e6922