Capa GPCR and NMUR in Fluid Homeostasis Figure 2. Desensitization and internalisation of capa-1-stimulated capaR. S2 cells were transfected with eYFP-tagged capaR, left un-treated or treated with capa-1, and viewed by confocal microscopy after immunocytochemistry with anti-GFP antibody. (A) Control. (B) capa-1 stimulated, 15 min. (C) sample was incubated for 15 minutes with capa-1, washed three times with culture medium followed by 30 minutes incubation in culture PLoS ONE | www.plosone.org 5 January 2012 | Volume 7 | Issue 1 | e29897