SMURF1 Amplification in Pancreatic Cancer Figure 1. Focal amplification of 7q22.1 in pancreatic cancer spans SMURF1. (A) A minimal amplicon is defined by four pancreatic cancer specimens (AsPC-1 and three xenografts). Starting from bottom: chr 7 ideogram; Heatmap of DNA copy number (red indicates gain) for the four specimens across the 7q21-q22 region (91–101 Mb); Scatter plot of DNA copy number log2 ratios across 7q21.3-q22.1 (96–100 Mb), overlaid with the cghFLasso [34] called ratios (red line); Screen shot of the corresponding locus from the UCSC genome browser. The dashed lines bracket the 325 Kb minimal amplicon, which spans SMURF1. (B) SMURF1 is overexpressed when gained/amplified. Box plots show 25th, 50th (median) and 75th percentile transcript levels (assayed by Agilent 44K array) for specimens with (red) or without (gray) 7q21-q22 gain, for SMURF1 and its nearest gene neighbors. Note, KPNA7 was not represented on the array. *, P,0.05; **, P,0.01; ***, P,0.001 (Mann-Whitney U-test). (C) FISH reveals SMURF1 amplification in primary pancreatic cancers. Shown are two pancreatic cancers with SMURF1 amplification (center, and right), along with a non-amplified control (BxPC3 cells, left). SMURF1 locus probe (red); control chr 7 centromere (CRP7) probe (green). doi:10.1371/journal.pone.0023924.g001 PLoS ONE | www.plosone.org 3 August 2011 | Volume 6 | Issue 8 | e23924