Lipid Raft Aggregation in Epithelial Cells and Mtb Figure 9. Treatment of epithelial cells with Filipin III disrupts mycobacterial-induced LR aggregation. A549 cells were treated with cholesterol-binding, LR-disruption agent Filipin III and LR aggregation observed for controls and infections with all three live Mtb strains. Confocal microscopy demonstrated an absence of CT-B puncta at 6 and 24 hpi for controls (A) and live Mtb strains (B). Images were collected at 63x magnification. Infections were performed in triplicate and repeated three times. doi:10.1371/journal.pone.0045028.g009 PLOS ONE | www.plosone.org 10 September 2012 | Volume 7 | Issue 9 | e45028