Apoptotic Activated T Cells Inhibit HIV in DCs Figure 3. Reduced percentage of HIV-infected DCs detected after co-culturing with ApoAct but not ApoRest. Immature DCs were exposed to HIVBaL (HIV), apoptotic anti-CD3– and anti-CD28–activated allogeneic CD4+ T cells (ApoAct), ApoAct in the presence of HIVBaL (ApoAct+HIV), apoptotic anti-CD3– and anti-CD28–activated allogeneic CD4+ T cells infected with HIVBaL (ApoInf), or ApoInf in the presence of exogenous virus (ApoInf+HIV). The percentage of infected DCs was assessed by flow cytometry analysis of intracellular p24 staining after 7 days. (A) Representative results from one donor and (B) paired results from eleven donors, both after 7 days of infection, are shown. (C) The average percentage of p24+ DCs 6 SD after 7 days in eight donors is shown. (D) Data comparing the percentage of p24+ DCs in the presence of HIVBaL after co-culturing with ApoAct (ApoAct+HIV) and apoptotic resting CD4+ T cells (ApoRest+HIV). Results are shown as the mean 6 SD of p24+ DCs from additional paired eight donors. Significant differences were assessed by the non-parametric Wilcoxon test and are indicated by *p,0.05, **p,0.01, and ***p,0.001. (E) The number of viral DNA copies/100 cells as assessed by quantitative real-time PCR for three donors is shown. (F) Integration of HIV DNA was assessed by Alu-PCR. Data depict five different donors. Significant differences were assessed by the non-parametric test and are indicated by *p,0.05, **p,0.01. doi:10.1371/journal.pone.0021171.g003 PLoS ONE | www.plosone.org 7 June 2011 | Volume 6 | Issue 6 | e21171