miR-210 Regulates ISCU Figure 3. MiR-210 increases the formation of ROS in MCF7 and HCT116 cell lines. A, in normoxia, transfection of mimic-210 significantly increases superoxide production at 48 hrs as measured by MitoSox staining. Representative images from mimic-210 transfected cells are shown on the top panels and the mean 6 s.e.m. of approximately 300 cells are shown on the bottom panels (***, p,0.001). B, exposure of cells to 0.1% oxygen for 48 hrs enhances the production of superoxide; this effect is almost completely reversed upon transfection of anti-210. Representative images from miR-210 transfected are shown on the left panels and the mean 6 s.e.m. of approximately 300 cells are shown on the right panels (***, p,0.001). C, ROS production induced by mimic-210 is inhibited in MCF7 cells by cotrasfection with the ISCU2 expressing plasmid. Mean 6 s.e.m. of approximately 300 cells is shown (*** p,0.001). Bars = 10 mm. doi:10.1371/journal.pone.0010345.g003 PLoS ONE | www.plosone.org 5 April 2010 | Volume 5 | Issue 4 | e10345