Table 4. cont. miRNA Group ID C. elegans miRNA Human related miRNAs 59 Sequence Full Sequence miR-256 cel-miR-256 hsa-miR-1 miR-259 miR-266 miR-268 miR-269 miR-273 miR-785 miR-786 cel-miR-259 cel-miR-266 cel-miR-268 cel-miR-269 cel-miR-273 cel-miR-785 cel-miR-786 miR-267 cel-miR-267 miR-357 cel-miR-357 miR-787 cel-miR-787 miR-790 cel-miR-790 miR-791 cel-miR-791 hsa-miR-19b hsa-miR-1 hsa-miR-122 hsa-miR-206 hsa-miR-519a hsa-miR-519b-3p hsa-miR-519c-3p hsa-miR-216a hsa-miR-216b hsa-miR-31 hsa-miR-99a hsa-miR-99b hsa-miR-100 hsa-miR-31 hsa-miR-873 hsa-miR-31 hsa-miR-99a hsa-miR-99b hsa-miR-100 hsa-miR-302a hsa-miR-302b hsa-miR-302c hsa-miR-302d hsa-miR-545* hsa-miR-559 hsa-miR-18a* hsa-miR-18b* hsa-miR-365 hsa-miR-99a* hsa-miR-99b* hsa-miR-96 hsa-miR-183 hsa-miR-200a hsa-miR-514 hsa-miR-96 hsa-miR-182 hsa-miR-183 hsa-miR-200a hsa-miR-514 hsa-let-7a hsa-let-7b hsa-let-7c hsa-let-7e hsa-let-7f hsa-miR-556-5p hsa-miR-25* hsa-miR-31 hsa-miR-301a hsa-miR-301b hsa-miR-31 MicroRNAs of Worms and Flies Human related miRNAs 59 Sequence Full Sequence hsa-let-7g hsa-let-7i hsa-miR-98 hsa-miR-202 hsa-let-7a hsa-let-7b hsa-let-7c hsa-let-7d hsa-let-7e hsa-let-7f hsa-let-7g hsa-let-7i hsa-miR-98 hsa-miR-196a hsa-let-7a hsa-let-7b hsa-let-7c hsa-let-7d hsa-let-7e hsa-let-7f hsa-let-7g hsa-let-7i hsa-miR-98 hsa-miR-1 hsa-miR-122 hsa-miR-206 Table 4. cont. miRNA Group ID C. elegans miRNA miR-794 cel-miR-794 miR-795 cel-miR-795 miR-796 cel-miR-796 miR-797 miR-1018 miR-1020 miR-1022 cel-miR-797 cel-miR-1018 cel-miR-1020 cel-miR-1022 hsa-miR-499-3p hsa-miR-450b-3p hsa-miR-148b* hsa-miR-450b-3p 76 C. elegans miRNAs are 59 related to 98 human miRNAs (Dataset S10), and 22 nematode miRNAs are $70% identical over the full length of 46 human miRNAs (Dataset S11). Worm-human miRNAs with weaker identity (60–69.9%) over full sequence are detailed in Dataset S12. Group IDs correspond to C. elegans miRNAs with human related-sequences. doi:10.1371/journal.pone.0002818.t004 but ,70% overall similarity with at least one of their 59 sequence- related miRNAs (indicated in Dataset S1). 40 miRNAs have significant homology to sequence-related worm miRNAs only at the 59 end and thus were not included in the $70% homology lists compiled after full length sequence comparison (Table 1, Dataset S2). Conversely, not all miRNAs with similarity over the sequence length include 7 or more continuous identical nucleotides within the first 10 nt of the 59 end. 3 of the 45 miRNAs with $70% identity (cel-miR-78, cel-miR-270 and cel-miR-272) fail to comply with our criteria for 59 end family grouping and therefore are not included in the list of 59 end-related miRNAs in Dataset S1. 39 end sequences miRNA target sites with perfect complementarity to miRNA 39 ends and negligible pairing at the 59 end have not been miR-793 cel-miR-793 hsa-let-7g PLoS ONE | www.plosone.org 9 July 2008 | Volume 3 | Issue 7 | e2818