cyp6a17 Is Required for TPB in Drosophila Figure 7. cyp6a17 expression is regulated by PKA signaling pathway. (A) Temperature preference behavior of MB247.UAS-PKACA, cyp6a17- RNAi. Temperature preference profile and avoidance index (AI) are shown. Black bars, AIHigh; gray bars, AILow. Two asterisks, P,0.001. (B–D) Determination of expression level of cyp6a17 in the PKA signaling pathway mutant background. CrebB-17AS162 (B); rut1 (C); and dncM14/dnc 1(D). Relative transcript level was measured by real-time PCR (histogram). gapdh1 was used as internal control. The number of tests: N = 3,4. Asterisks, P,0.001 by t test. Error bars indicates S.E.M. doi:10.1371/journal.pone.0029800.g007 PLoS ONE | www.plosone.org 9 December 2011 | Volume 6 | Issue 12 | e29800