SOD3 Derived Cell Migration Figure 2. Inhibition of T-cell migration. Open bars represent LacZ animals and black bars represent SOD3 animals. Infiltration of CD3+ lymphocytes was inhibited in SOD3 animals 10 days after injury remaining at the level seen at earlier 3-day time point (206 magnification). doi:10.1371/journal.pone.0005786.g002 Figure 3. Anti-inflammatory effect of SOD3 affects predominantly macrophages in peritonitis model. Open bars represent LacZ animals and black bars represent SOD3 animals. (a) SOD3-mediated reduction in total leukocyte number in peritoneal lavage 18 hours after induction of inflammation. SOD3 treated animals had 55.66104 (64.1) cells/milliliters of lavage as compared to 91.66104 (66.7) found from LacZ control animals. (b) Analysis of different leukocyte subtypes showed strongest effect in macrophages although lymphocyte migration was also reduced. Monocyte accumulation in LacZ vs. SOD3 treatment was reduced from 33.56104 (61.8) to 10.96104 (62.5) cells/ml whereas lymphocytes were reduced from 11.46104 (62.2) to 7.66104 (61.2) cells/ml, and neutrophils from 47.06104 (64.7) to 37.56104 (62.9) cells/ml. doi:10.1371/journal.pone.0005786.g003 PLoS ONE | www.plosone.org 4 June 2009 | Volume 4 | Issue 6 | e5786