Phylogeny Orders HSE Evolution Figure 10. Evolutionary changes in numbers of HSEs superimposed on a phylogenetic tree of the 13 species. Tallies of total changes in all 7 genes surveyed are placed in the centers of the segments in which the changes occurred. The changes themselves, however, may have occurred at any time during the interval the segment represents. *One change, that for Hsp68, cannot be assigned to a specific segment because the published genomic sequence for D. willistoni lacks a proximal promoter region that can be aligned to those for the other species. doi:10.1371/journal.pone.0010669.g010 Figure 11. Sequence logos for nGAAn and nTTCn motifs. WebLogos were generated using the entire pool of available motifs, including duplications and multiple Hsp70 copies. doi:10.1371/journal.pone.0010669.g011 PLoS ONE | www.plosone.org 11 May 2010 | Volume 5 | Issue 5 | e10669