Akt1 in Mammary Development Figure 6. Akt1 is necessary for Btn1a1 expression following pseudopregnant hormonal stimulation. Representative whole mounts of pseudopregnant Akt1+/+ (A) and Akt12/2 (B) mammary glands, and H&E stained (C, D) mammary glands. Representative immunofluorescence of Akt1 and Btn1a1 in pseudopregnant Akt1+/+ (E, G, I) and Akt12/2 (F, H, J) mammary glands. Arrow indicates co-expression of Akt1 and Btn1a1 the in Akt1+/+ mammary gland epithelium. Btn1a1 gene expression as determined by real time RT-PCR in virgin and pseuodopregnant Akt1+/+ and Akt12/2 thoracic mammary glands (K). Exposure = pseudopregnancy. Scale bars for whole mounts and histology/immunohistochemistry indicate 1,000 mm and 100 mm, respectively. Data represented as mean plus standard error. Statistical analyses were conducted by a two-way ANOVA followed by a Tukey HSD test. **p,0.01 for Tukey HSD post-hoc test. n = 3–6 per genotype, per exposure. doi:10.1371/journal.pone.0024432.g006 PLoS ONE | www.plosone.org 9 September 2011 | Volume 6 | Issue 9 | e24432