Figure S6 Cell cycle analysis of isolated mouse islet cells. Representative FACS measurements of propidium iodide- stained nuclear DNA from islet cells isolated of (A) wild type mice and (B) PKCdKN transgenic mice and means + SEM from n = 3 independent experiments. (TIF) Acknowledgments We thank Sieglinde Haug for excellent technical help and Reiner Lammers for providing PKCdWT and PKCdKN INS-1E cells (SH and RL, Department of Internal Medicine, Division of Endocrinology, Diabetology, References 1. Rhodes CJ (2005) Type 2 diabetes-a matter of beta-cell life and death? Science 307: 380–384. 2. Lingohr MK, Dickson LM, McCuaig JF, Hugl SR, Twardzik DR, et al. (2002) Activation of IRS-2-mediated signal transduction by IGF-1, but not TGF-alpha or EGF, augments pancreatic beta-cell proliferation. Diabetes 51: 966–976. 3. Laybutt DR, Preston AM, Akerfeldt MC, Kench JG, Busch AK, et al. (2007) Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes. 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PLoS ONE | www.plosone.org 10 December 2011 | Volume 6 | Issue 12 | e28828