37. Lints R, Hall DH (2009) Male muscle system, male-specific muscles. In WormAtlas doi:103908/wormatlas25. 38. Gower NJ, Walker DS, Baylis HA (2005) Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males. Mol Biol Cell 16: 3978–3986. 39. Bennett MV, Zukin RS (2004) Electrical coupling and neuronal synchronization in the Mammalian brain. Neuron 41: 495–511. 40. Bennett MV, Barrio LC, Bargiello TA, Spray DC, Hertzberg E, et al. (1991) Gap junctions: new tools, new answers, new questions. Neuron 6: 305–320. 41. Nagel G, Brauner M, Liewald JF, Adeishvili N, Bamberg E, et al. (2005) Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses. Curr Biol 15: 2279–2284. 42. Nagel G, Szellas T, Huhn W, Kateriya S, Adeishvili N, et al. (2003) Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc Natl Acad Sci U S A 100: 13940–13945. 43. Nakai J, Ohkura M, Imoto K (2001) A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat Biotechnol 19: 137–141. 44. Reiner DJ, Weinshenker D, Tian H, Thomas JH, Nishiwaki K, et al. (2006) Behavioral genetics of caenorhabditis elegans unc-103-encoded erg-like K(+) channel. J Neurogenet 20: 41–66. 45. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, et al. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 17: 969–973. 46. Baird GS, Zacharias DA, Tsien RY (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A 97: 11984–11989. 47. Zhang F, Prigge M, Beyriere F, Tsunoda SP, Mattis J, et al. (2008) Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat Neurosci 11: 631–633. 48. Reiner DJ, Weinshenker D, Thomas JH (1995) Analysis of dominant mutations affecting muscle excitation in Caenorhabditis elegans. Genetics 141: 961–976. 49. Peters MA, Teramoto T, White JQ, Iwasaki K, Jorgensen EM (2007) A calcium wave mediated by gap junctions coordinates a rhythmic behavior in C. elegans. Curr Biol 17: 1601–1608. 50. Davidson JS, Baumgarten IM (1988) Glycyrrhetinic acid derivatives: a novel class of inhibitors of gap-junctional intercellular communication. Structure- activity relationships. J Pharmacol Exp Ther 246: 1104–1107. 51. Schneider NL, Stengl M (2006) Gap junctions between accessory medulla neurons appear to synchronize circadian clock cells of the cockroach Leucophaea maderae. J Neurophysiol 95: 1996–2002. 52. Bao L, Samuels S, Locovei S, Macagno ER, Muller KJ, et al. (2007) Innexins form two types of channels. FEBS Lett 581: 5703–5708. 53. Raizen DM, Lee RY, Avery L (1995) Interacting genes required for pharyngeal excitation by motor neuron MC in Caenorhabditis elegans. Genetics 141: 1365–1382. 54. Sattelle DB, Culetto E, Grauso M, Raymond V, Franks CJ, et al. (2002) Functional genomics of ionotropic acetylcholine receptors in Caenorhabditis elegans and Drosophila melanogaster. Novartis Found Symp 245: 240–257; discussion 257- 260, 261-244. 55. Altun ZF, Chen B, Wang ZW, Hall DH (2009) High resolution map of Caenorhabditis elegans gap junction proteins. Dev Dyn 238: 1936–1950. 56. Starich T, Sheehan M, Jadrich J, Shaw J (2001) Innexins in C. elegans. Cell Commun Adhes 8: 311–314. 57. Sulston JE, Horvitz HR (1977) Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev Biol 56: 110–156. 58. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71–94. 59. Liu Q, Chen B, Gaier E, Joshi J, Wang ZW (2006) Low conductance gap junctions mediate specific electrical coupling in body-wall muscle cells of Caenorhabditis elegans. J Biol Chem 281: 7881–7889. 60. Garcia LR, Sternberg PW (2003) Caenorhabditis elegans UNC-103 ERG-like potassium channel regulates contractile behaviors of sex muscles in males before and during mating. J Neurosci 23: 2696–2705. 61. Franks CJ, Murray C, Ogden D, O’Connor V, Holden-Dye L (2009) A comparison of electrically evoked and channel rhodopsin-evoked postsynaptic potentials in the pharyngeal system of Caenorhabditis elegans. Invert Neurosci 9: 43–56. 62. Baier H, Scott EK (2009) Genetic and optical targeting of neural circuits and behavior—zebrafish in the spotlight. Curr Opin Neurobiol 19: 553–560. 63. Guo ZV, Hart AC, Ramanathan S (2009) Optical interrogation of neural circuits in Caenorhabditis elegans. Nat Methods 6: 891–896. Schema of the Spicule Insertion Circuit 64. Schroeder CE, Wilson DA, Radman T, Scharfman H, Lakatos P (2010) Dynamics of Active Sensing and perceptual selection. Curr Opin Neurobiol 20: 172–176. 65. Cronin CJ, Mendel JE, Mukhtar S, Kim YM, Stirbl RC, et al. (2005) An automated system for measuring parameters of nematode sinusoidal movement. BMC Genet 6: 5. 66. Shadmehr R, Smith MA, Krakauer JW (2010) Error correction, sensory prediction, and adaptation in motor control. Annu Rev Neurosci 33: 89–108. 67. Yu H, Pretot R, Burglin T, Sternberg P (2003) Distinct roles of transcription factors EGL-46 and DAF-19 in specifying the functionality of a polycystin- expressing sensory neuron necessary for C. elegans male vulva location behavior. Development 130: 5217–5227. 68. Peden E, Barr M (2005) The KLP-6 kinesin is required for male mating behaviors and polycystin localization in Caenorhabditis elegans. Curr Biol 15: 394–404. 69. Jauregui A, Barr M (2005) Functional characterization of the C. elegans nephrocystins NPHP-1 and NPHP-4 and their role in cilia and male sensory behaviors. Exp Cell Res 305: 333–342. 70. Bae Y-K, Lyman-Gingerich J, Barr M, Knobel K (2008) Identification of genes involved in the ciliary trafficking of C. elegans PKD-2. Developmental Dynamics 237: 2021–2029. 71. White JG, Southgate E, Thomson JN, Brenner S (1986) The Structure of the Nervous System of the Nematode Caenorhabditis elegans. Phil Trans Royal Soc London Series B, Biol Scien 314: 1–340. 72. Macosko EZ, Pokala N, Feinberg EH, Chalasani SH, Butcher RA, et al. (2009) A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. elegans. Nature 458: 1171–1175. 73. Chen B, Liu Q, Ge Q, Xie J, Wang ZW (2007) UNC-1 regulates gap junctions important to locomotion in C. elegans. Curr Biol 17: 1334–1339. 74. Wagenaar DA, Hamilton MS, Huang T, Kristan WB, French KA (2010) A hormone-activated central pattern generator for courtship. Curr Biol 20: 487–495. 75. Grillner S, Wallen P, Saitoh K, Kozlov A, Robertson B (2008) Neural bases of goal-directed locomotion in vertebrates—an overview. Brain Res Rev 57: 2–12. 76. Grillner S, Jessell TM (2009) Measured motion: searching for simplicity in spinal locomotor networks. Curr Opin Neurobiol 19: 572–586. 77. Nisenbaum ES, Wilson CJ (1995) Potassium currents responsible for inward and outward rectification in rat neostriatal spiny projection neurons. J Neurosci 15: 4449–4463. 78. Kreitzer AC, Malenka RC (2008) Striatal plasticity and basal ganglia circuit function. Neuron 60: 543–554. 79. Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998) Inwardly rectifying potassium (IRK) currents are correlated with IRK subunit expression in rat nucleus accumbens medium spiny neurons. J Neurosci 18: 6650–6661. 80. Wilson CJ, Kawaguchi Y (1996) The origins of two-state spontaneous membrane potential fluctuations of neostriatal spiny neurons. J Neurosci 16: 2397–2410. 81. Grillner S, Hellgren J, Menard A, Saitoh K, Wikstrom MA (2005) Mechanisms for selection of basic motor programs—roles for the striatum and pallidum. Trends Neurosci 28: 364–370. 82. Hodgkin JA, Horvitz HR, Brenner S (1979) Nondisjunction mutants of the nematode Caenorhabditis elegans. Genetics 91: 67–94. 83. Park EC, Horvitz HR (1986) Mutations with dominant effects on the behavior and morphology of the nematode Caenorhabditis elegans. Genetics 113: 821–852. 84. Schnabel H, Schnabel R (1990) An Organ-Specific Differentiation Gene, pha-1, from Caenorhabditis elegans. Science 250: 686–688. 85. Edwards S, Charlie N, Milfort M, Brown B, Gravlin C, et al. (2008) A novel molecular solution for ultraviolet light detection in Caenorhabditis elegans. PLoS Biol 6: e198. doi:10.1371/journal.pbio.0060198. 86. Bargmann CI, Avery L (1995) Laser killing of cells in Caenorhabditis elegans. Methods Cell Biol 48: 225–250. 87. Fang-Yen C, Wasserman S, Sengupta P, Samuel AD (2009) Agarose immobilization of C. elegans. Worm Breeder’s Gazette 18: 32. 88. Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, et al. (2004) Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res 14: 2162–2168. 89. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J (2001) Effectiveness of specific RNA-mediated interference through ingested double- stranded RNA in Caenorhabditis elegans. Genome Biol 2: RESEARCH0002. PLoS Genetics | www.plosgenetics.org 20 March 2011 | Volume 7 | Issue 3 | e1001326