Nestin and Steroid Receptor Figure 9. EM immunogold replica technique shows specific association of GR containing complexes with vimentin filaments. Cytoskeletons of C6D8 cells were fixed, double labelled with antibodies and immunogold particles, postfixed, and handled the same way as described in legend to Figure 8. (A) The labeling technique clearly identifies vimentin containing IFs (10 nm immunogold particles); labeling of actin with 5 nm immunogold particles; (B1–B3) double labelling with antibodies to vimentin (10 nm immunogold particles) and GR (5 nm immunogold particles) shows specific association of GR with vimentin containing filaments. doi:10.1371/journal.pone.0006084.g009 PLoS ONE | www.plosone.org 11 June 2009 | Volume 4 | Issue 6 | e6084