proximal to B–E (through transition zones and distal dendrites). Transition zones appear identical in N2 and daf-25 worms, with Y- links (arrow) and the internal apical ring (arrowhead) clearly visible and intact. Scale bars; 200 nm. Found at: doi:10.1371/journal.pgen.1001199.s003 (9.08 MB TIF) Figure S4 Alignment of DAF-25 with Ankmy2. C. elegans (Ce) DAF-25 was aligned with Homo sapiens (Hs), Bos taurus (Bt), Mus musculus (Mm), and Danio rerio (Dr). The red bar indicates the ankyrin repeat domain and the blue bar indicates the zinc finger MYND domain. White font on black background indicates conservation in all five species, white font on grey indicates four, and black font on grey indicates three. Ankmy2 is very well conserved among chordates, with identity percentages compared to human Ankmy2 of 93% for cow, 88% for mouse, and 76% for zebrafish while DAF-25 shares 32% identity. Found at: doi:10.1371/journal.pgen.1001199.s004 (2.18 MB TIF) Figure S5 The daf-25 transcript including allele and UTR information. Displayed is the sequence of the daf-25 transcript including the molecular lesions in the four daf-25 alleles. A line over the sequence indicates the extent of the deletion. A line under the amino acid sequence indicates the two protein domains including the ankyrin repeat domain in the first half of the sequence and the zinc-finger MYND domain near the C-terminus of the sequence. Found at: doi:10.1371/journal.pgen.1001199.s005 (3.21 MB TIF) Figure S6 Many cilia targeted proteins localize normally in daf- 25(m362). Shown are the localization patterns of the translational fusion constructs BBS-8::GFP, CHE-2::GFP, CHE-11::GFP, CHE-13::GFP, OSM-5::GFP and XBX-1::GFP. All six of these GFP-tagged proteins localize normally to the cilia in both N2 and daf-25(m362) mutants, indicating that DAF-25 is unlikely to be a References 1. 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