DAF-25/Ankmy2 Functions in Ciliary cGMP Signaling Figure 5. DAF-25 is required for the localization of a guanylyl cyclase (DAF-11) to cilia. The guanylyl cyclase DAF-11::GFP translational fusion protein was expressed in both N2 and daf-25(m362) genetic backgrounds. In wild type (A), DAF-11::GFP was localized to the ASI, ASJ or ASK sensory cilia, but was limited to the distal end of the dendrites (indicated by arrow) and largely excluded from basal body-ciliary structures in daf- 25(m362) cilia (B). Normal ciliary localization was seen for the transient receptor potential channel (TRPV4) OSM-9::GFP reporter gene in both wild type PLoS Genetics | www.plosgenetics.org 7 November 2010 | Volume 6 | Issue 11 | e1001199