29. Kim J, Irvine KD, Carroll SB (1995) Cell recognition, signal induction, and symmetrical gene activation at the dorsal-ventral boundary of the developing Drosophila wing. Cell 82: 795–802. 30. Speicher SA, Thomas U, Hinz U, Knust E (1994) The Serrate locus of Drosophila and its role in morphogenesis of the wing imaginal discs: control of cell proliferation. Development 120: 535–544. 31. Furriols M, Bray S (2001) A model response element detects Suppressor of Hairless-dependent molecular switch. Curr Biol 11: 60–64. 32. Lai EC (2002) Keeping a good pathway down: transcriptional repression of Notch pathway target genes by CSL proteins. EMBO Rep 9: 840–845. 33. Klein T, Seugnet L, Haenlin M, Martinez-Arias A (2000) Two different activities of Suppressor of Hairless during wing development in Drosophila. Development 127: 3553–3566. 34. Bachmann A, Knust E (1998) Dissection of cis-regulatory elements of the Drosophila gene Serrate. Dev Genes Evol 208: 346–351. 35. Lai EC, Roegiers F, Qin X, Jan YN, Rubin GM (2005) The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta. Development 132: 2319–2332. 36. Le Borgne R, Remaud S, Hamel S, Schweisguth F (2005) Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila. PLoS Biol 3: e96. 37. Wang W, Struhl G (2005) Distinct roles for Mind bomb, Neuralized and Epsin in mediating DSL endocytosis and signaling in Drosophila. Development 132: 2883–2894. 38. Lei L, Xu A, Panin V, Irvine KD (2003) An O-fucose site in the ligand binding domain inhibits Notch activation. Development 130: 6411–6421. 39. Klein T, Martinez-Arias AM (1998) Different spatial and temporal interactions between Notch, wingless, and vestigial specify proximal and distal pattern elements of the wing in Drosophila. Developmental Biology 194: 196–212. 40. Klein T, Brennan K, Martinez-Arias AM (1997) An intrinsic dominant negative activity of serrate that is modulated during wing development in Drosophila. Developmental Biology 189: 123–134. 41. Yaron A, Sprinzal D (2011) The cis side of juxtacrine signaling: a new role in the development of the nervous system. Trends Neurosci 35: 230–239. 42. Dahmann C, Basler K (1999) Compartment boundaries: at the edge of development. Trends Genet 15: 320–326. Sequential Signalling at the Fng Boundary 43. Grammont M, Irvine KD (2001) fringe and Notch specify polar cell fate during Drosophila oogenesis. Development 128: 2243–2253. 44. Struhl G, Greenwald I (1999) Presenilin is required for activity and nuclear access of Notch in Drosophila [see comments]. Nature 398: 522–525. 45. Lecourtois M, Schweisguth F (1995) The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes And Development 9: 2598–2608. 46. Thomas U, Speicher SA, Knust E (1991) The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs. Development 111: 749–761. 47. Bang AG, Bailey AM, Posakony JW (1995) Hairless promotes stable commitment to the sensory organ precursor cell fate by negatively regulating the activity of the Notch signaling pathway. Developmental Biology 172: 479– 494. 48. Cohen B, McGuffin ME, Pfeifle C, Segal D, Cohen SM (1992) apterous, a gene required for imaginal disc development in Drosophila encodes a member of the LIM family of developmental regulatory proteins. Genes And Development 6: 715–729. 49. Williams JA, Paddock SW, Vorwerk K, Carroll SB (1994) Organization of wing formation and induction of a wing-patterning gene at the dorsal/ventral compartment boundary. Nature 368: 299–305. 50. Bachmann A, Knust E (1998) Positive and negative control of Serrate expression during early development of the Drosophila wing. Mech Dev 76: 67–78. 51. Duffy JB, Harrison DA, Perrimon N (1998) Identifying loci required for follicular patterning using directed mosaics. Development 125: 2263–2271. 52. Yeh E, Gustafson K, Boulianne GL (1995) Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proceedings Of The National Academy Of Sciences Of The United States Of America 92: 7036– 7040. 53. Greco V, Hannus M, Eaton S (2001) Argosomes: a potential vehicle for the spread of morphogens through epithelia. Cell 106: 633–645. 54. Lee YS, Nakahara K, Pham JW, Kim K, He Z, et al. (2004) Distinct roles for Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117: 69–81. PLOS ONE | www.plosone.org 16 November 2012 | Volume 7 | Issue 11 | e49007