Sox9-Haploinsufficiency Causes Glucose Intolerance Figure 5. Sox9-haploinsufficient mice are glucose-intolerant. Body weight (A), random fed blood glucose levels (B), and fasting blood glucose levels (C) do not significantly differ in Sox9+/Dpan and control mice (n = 10), except for fasting blood glucose levels in Sox9+/Dpan mice at 36 weeks of age. (C) The dashed line indicates the threshold at which blood glucose levels are considered diabetic. Following an intraperitoneal glucose challenge, Sox9+/Dpan mice exhibit elevated blood glucose levels compared to control mice at 6 weeks (D; n = 20), 12 weeks (E; n = 18), and 36 weeks (F; n = 8) of age. (G) Blood glucose levels in response to an intraperitoneal glucose challenge are similar in Pdx1-Cre mice and wild-type littermates (n = 11). Values are shown as mean 6 S.E.M.; * = P,0.05; ** = P,0.01. doi:10.1371/journal.pone.0023131.g005 PLoS ONE | www.plosone.org 7 August 2011 | Volume 6 | Issue 8 | e23131