TB 15), or its respective isotype controls (BD Pharmingen) for a further 30 min. All the samples were run on a Becton Dickinson LSR-II and data were analyzed using FACSDiva v5.0.1 software. Cells were gated on lymphocytes based on characteristic forward and side scatter profiles. Individual cell populations were identified according to their presence of specific fluorescent-labeled anti- bodies. All the analyses were performed with a minimum acquisition of 100,000 events. Our laboratory routinely uses the Becton-Dickinson Company LSR II flow cytometer fluorescence minus one (FMO) technology comparing the isotype control verses the fluorescence of the subpopulations evaluated as an internal quality control in addition to the standard isotype verses isotype control. We have verified that no significant differences exist in values between our isotype verse isotype control compared to our isotype verses the fluorescence of the subpopulations evaluated in our study. 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