LTP-Related Gene Networks Figure 9. IPA network analysis at 24 h. The three highest scoring networks from the LRG list are shown. See Figure 5 legend for further details. doi:10.1371/journal.pone.0040538.g009 Discussion We have used Ingenuity Pathway Analysis and DAVID to interpret how the differentially expressed genes identified at 20 min, 5 h and 24 h post-LTP induction at perforant path synapses in vivo are related. This analysis has confirmed that the persistence of LTP is associated with ongoing regulation of gene expression and has highlighted both an integrated network of regulatory mechanisms as well as effector mechanisms likely to contribute directly to the consolidation of LTP. Our analysis has identified regulation of G-protein coupled signalling, calcium dynamics and ion channels as important biological functions that are regulated 5 h post-LTP induction, PLoS ONE | www.plosone.org 10 July 2012 | Volume 7 | Issue 7 | e40538