Perspective Spindle Positioning, Meiotic Nonreduction, and Polyploidy in Plants Sebastien Andreuzza, Imran Siddiqi* Centre for Cellular and Molecular Biology, Hyderabad, India Polyploidy, the state of having more than two sets of chromosomes, is common in flowering plants (angiosperms), including the major crops [1]. Indeed, it is estimated that 30%–80% of the angiosperms are polyploids [2], and most diploid plant species, including Arabidopsis thaliana, show evidence of genome duplication in their ancestry [3]. Polyploidy is accompanied by genome-wide changes in gene expression and epigenetic modifications, leading to phenotypic diversity and rapid adaptation [4]. Doubling of chromosome number can also rescue and stabilize interspecific hy- brids that would otherwise show a high degree of sterility due to failures in meiosis. Thus, polyploidy is thought to be a driving force in plant evolution and speciation [5]. In this issue of PLoS Genetics, d’Erfurth and colleagues report on the molecular charac- terization of the AtPS1 gene and show that lesions in the gene result in unreduced gametes, which are believed to be an important step in polyploidization [6]. While polyploids can originate by an increase of chromosome number either during somatic growth or during meiosis, the major route is now considered to be via the formation of unreduced gametes [7]. The formation of 2n gametes resulting from failure of reduction during meiosis occurs in several plant species and can give rise to triploids that can serve as a bridge to the establishment of an even set of chromosomes in subsequent generations [8]. Unreduced gametes have also been used in breeding schemes for crop im- provement wherein beneficial traits have been introduced from diploid relatives into cultivated polyploid species [9]. The paper by d’Erfurth et al. [6] provides the first molecular insight into high-efficien- cy unreduced pollen formation in plants based on analysis of mutants in the Arabi- dopsis gene AtPS1 that produce diploid pollen. A range of meiotic abnormalities can give rise to unreduced gamete forma- tion. These can be broadly classified as first or second division restitution (FDR and SDR, respectively) [2]. The occurrence of parallel spindles at meiosis II, resulting in a single plane of cell division and formation of dyad spores, is a frequently found mecha- nism for 2n gamete formation and is genetically equivalent to FDR in that it results in retention of heterozygosity for centromeric markers (Figure 1). The parallel spindle 1 (ps1) mutant of potato, which produces unreduced pollen by such a mechanism, was described more than 80 years ago [10] and has been used in potato breeding for introgression of beneficial traits in cultivated strains [11]. While the occur- rence of unreduced gamete formation in plants has long been recognized, the molec- ular mechanisms leading to diploid gamete formation have only recently begun to be uncovered [12,13]. d’Erfurth et al. identified the AtPS1 gene using a novel bioinformatics approach. They looked for genes whose expression pattern in microarrays correlated with that of known meiotic genes. Subsequent analysis showed that T-DNA insertions in AtPS1 result in the production of diploid pollen, and triploidy in as many as 30% of progeny. The authors then show, by elegant genetic and cytological experi- ments, that the atps1 mutant phenotype results from the positioning of the spindles at metaphase II in a parallel orientation instead of in the normal tetrahedral configuration. In most angiosperms, in- cluding Arabidopsis, cytokinesis at the time of meiosis occurs only after the second round of chromosome segregation. The occurrence of parallel spindles at meiosis II in atps1 thus results in the formation of one plane of division, and gives rise to dyads containing diploid spores instead of a tetrad of haploid spores (Figure 1). Interestingly, AtPS1 is a plant-specific gene that encodes a protein containing an N-terminal Forkhead Associated (FHA) domain. FHA domains are phosphopro- tein binding modules and are found in proteins that regulate signal transduction pathways in several different contexts: plant meristem homeostasis, DNA repair, and cell cycle control [14]. The identifi- cation of AtPS1 opens a new window on the control of meiosis II, particularly in relation to spindle orientation. The spindle pole body (SPB) and the centrosome are the yeast and animal microtubule organizing centers (MTOCs), respectively. MTOCs nucleate microtu- bules and orient the spindle during cell division [15]. Within the SPB and centro- some is a pair of centrioles that contain c- tubulin, the site of nucleation of microtu- bules [16]. Although c-tubulin is con- served in eukaryotes, land plants lack a conspicuous MTOC in the form of a centrosome. Nucleation of microtubules occurs in the cortical cytoplasm and on the nuclear envelope from sites along existing microtubules that are marked by c-tubulin [17]. Microtubule nucleation in plants therefore appears to be spatially distribut- ed within the cell. A number of reports point to c-tubulin as the plant MTOC, but the precise mechanism underlying orien- tation of the spindle and its relation to spindle biogenesis during cell division in plants remains to be established [18]. The identification of AtPS1 and its role in controlling spindle orientation during mei- osis II represents an important step in understanding the control of spindle positioning in plants, and determination of its mode of action would be of continued interest. Importantly, because unreduced gametes are of agronomic value, the discovery of AtPS1 may also benefit crop biotechnology. Citation: Andreuzza S, Siddiqi I (2008) Spindle Positioning, Meiotic Nonreduction, and Polyploidy in Plants. PLoS Genet 4(11): e1000272. doi:10.1371/journal.pgen.1000272 Published November 28, 2008 Copyright: 2008 Andreuzza, Siddiqi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. * E-mail: imran@ccmb.res.in PLoS Genetics | www.plosgenetics.org 1 November 2008 | Volume 4 | Issue 11 | e1000272