AGO10 and Floral Stem Cell Termination Figure 2. AGO10 and AGO1 promote floral determinacy. (A) An ag-10 flower; most ag-10 flowers were similar to wild-type ones. (B) An ag-10 ago10-13 flower; the gynoecia of ag-10 ago10-13 flowers were bulged and contained ectopic floral organs internally. (C) A flower of ag-10 ago10-13 pAGO10::AGO10-FLAG showing that AGO10 rescued the floral determinacy defects of ag-10 ago10-13. (D) Representative siliques from ag-10 plants. Most were similar to wild-type siliques, while some were constricted on one side but still long and thin. Bulged siliques with internal floral organs (represented by the one on the left) were observed at a low frequency (usually 1-2 per plant). (E) Siliques from ag-10 ago10-13 plants. 100% of the siliques on an ag-10 ago10-13 plant were bulged. Occasionally, the primary carpels that make up a silique were not properly fused, revealing the internal organs. (F) An ago10-13 ago1-11/+ gynoecium with internal floral organs (arrow) bursting out of the gynoecium. (G, H) Longitudinal sections PLoS Genetics | www.plosgenetics.org 5 March 2011 | Volume 7 | Issue 3 | e1001358