Inhibition of EGFR Localization to Mitochondria Figure 6. Downregulation of pEGFR and phospho-c-Src in mitochondria in hUCBSC treatments. (A) Control and hUCBSC-treated U251, U87 and 5310 cells were labeled with pEGFR and Mito Tracker green and processed for immunofluorescence. (B) Control and hUCBSC-treated U251 and 5310 tissue sections were labeled with pEGFR and Mito Tracker green and processed for immunofluorescence. pEGFR was conjugated with Alexa Fluor 594 (red) secondary antibody (n$3; bar = 100 mm). (C) Control and hUCBSC-treated U251, U87 and 5310 cells were labeled with phospho-c-Src and Mito Tracker green and processed for immunofluorescence. (D) Control and hUCBSC-treated U251 and 5310 tissue sections were labeled with phospho-c-Src and Mito Tracker green and processed for immunofluorescence. Phospho-c-Src was conjugated with Alexa Fluor 594 (red) secondary antibody (n$3; bar = 100 mm). doi:10.1371/journal.pone.0031884.g006 reported. Mitochondria are involved in diverse cellular and metabolic processes including proliferation, programmed cell death execution and signal transduction [44–46]. Recent reports reveal that there exists an integrated signal system in the mitochondrion to coordinate the various molecular messages that enter and exit the mitochondrion according to the diverse needs of PLoS ONE | www.plosone.org 9 February 2012 | Volume 7 | Issue 2 | e31884