TGACCCAGATCATGTTTGAGA-39 and Reverse 59-AGTC- CATCACGATGCCAGT-39 for human ACTB (NM_001101.3); Forward 59-AGTGTGACGTTGACATCCGT-39 and Reverse 59- CCACCGATCCACACAGAGTA-39 for mouse Actb (NM_007393.3); Forward 59-CACCCGCGAGTACAACCTTC- 39 and Reverse 59-CCCATACCCACCATCACACC-39 for rat Actb (NM_031144.2); Forward 59-ACGAAATCAACAACC- CCGTA-39 and Reverse 59-GGCAGAACGACTCGGTTATC- 39 for mouse MT-Co2 (NC_005089.1). b-actin gene (ACTB or Actb) was used as the QPCR internal control for cell and tissue RNA samples. Cytochrome C oxidase II (COXII) gene (MT-Co2) was used as the internal control for mitochondrial polysome-associated RNA samples. The amplified products were checked by separation on a 2% agarose gel. Western blotting Tissues were homogenised in lysis buffer [20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Nonidet P 40, 0.1% (w/v) SDS, 1% (w/v) sodium deoxycholate, 1 mM NaF, 1 mM Na3VO4, 1% (v/v) protease inhibitor cocktails (Roche, Mannheim, Germany)]. 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