Sans in Fly Development Figure 7. Sans co-localizes with the syntaxin Avalanche to early endocytic vesicles. A: Clones of Sans245/Sans245 mutant follicle cells marked by the absence of nuclear GFP (green) of stage 10-egg chambers stained for F-actin (red) and Sans (blue). Sans localizes to intracellular punctate structures of follicle cells. B–D: Clones of follicle cells expressing YFP-Sans (Act5C.Gal4, UAS-YFP-Sans) labeled by YFP-Sans (green) and stained for F-actin (red). The white line in (D) delineates the clone border. YFP-Sans localizes to punctate structures enriched beneath the apical plasma membrane. Note that the YFP-Sans punctate structures are enlarged in comparison to the endogeneous Sans punctate structures. E,F: Wild- type stage 10-egg chambers stained for Avalanche (Avl, red) and Sans (green). Sans partially co-localizes with Avl in punctate structures. (A,F) show top views and (B–E) show cross-sectional views of the follicle epithelium (apical is to the top). Scale bars: A,C–F: 10 mm; B: 20 mm. doi:10.1371/journal.pone.0004753.g007 PLoS ONE | www.plosone.org 8 March 2009 | Volume 4 | Issue 3 | e4753