Regulation of EGFR Signal Transduction Figure 1. DHA reduces localization of EGFR to lipid rafts. A) YAMC cells were untreated (control) or treated with 50 mM BSA-complexed fatty acids (LA or DHA) for 72 h (12 flasks per treatment). For the final 16–18 h, cells were incubated with low serum media (0.5% FBS) with the same concentration of fatty acids. Cells were harvested from each flask, pooled (n = 12), and the plasma membrane (PM) was isolated. Following isolation, PLoS ONE | www.plosone.org 3 June 2012 | Volume 7 | Issue 6 | e39682