Human Genes Required for Influenza Replication Figure 5. Effect of miRNA inhibition on host protease gene expression 24 h post-miRNA inhibitor treatment. Host cell miRNAs of interest were evaluated for their effect on host gene hits by qPCR. A549 cells were treated with the appropriate miRNA inhibitor (25 nM) for 24 hours. Cellular RNA was isolated 24 hpi and evaluated by qPCR for host gene expression using a SYBRgreen assay with gene-specific primers. Gene expression was compared to cells transfected with siNEG (for siRNA) or NEG (non-targeting miRNA inhibitor) at the equivalent concentration. Data is normalized to GAPDH expression. miRNAs indicated on the x-axis refer to inhibition of those miRNAs. A: ADAMTS7 expression levels, B: CPE, C: DPP3, D: MST1, E: PRSS12. Data is representative of two independent experiments. (*p,0.05 versus siRNA treatment.). doi:10.1371/journal.pone.0037169.g005 PLoS ONE | www.plosone.org 5 May 2012 | Volume 7 | Issue 5 | e37169