CHX and Lexa Combination-Induced Apoptosis in HCC Figure 1. Lexa and CHX combination treatment induces apoptosis in HCC cells. A, Huh7 cells were treated with TNF-a (10 ng/ml) or Lexa (1 mg/ml) for indicated times. Cell lysates were prepared and subjected to Western blotting to detect the expression of IkB-a and phospho-IkB-a. b- actin protein levels were used as an equal protein loading control (Lexa, lexatumumab). B, Huh7 cells were treated with DMSO (Con), CHX (10 mg/ml), Lexa (1 mg/ml), or a combination of Lexa (1 mg/ml) and CHX (10 mg/ml). Apoptosis was measured by nuclear dye Hoechst 33258 staining to label DNA PLoS ONE | www.plosone.org 3 February 2011 | Volume 6 | Issue 2 | e16966