Resveratrol Inhibit LPS-Induced Activation by mTOR Figure 8. mTOR is involved in resveratrol-attenuated the production of the proinflammatory cytokine IL-1b at the transcriptional and translational levels in BV-2 cells. Panel A shows the immunofluorenscence images for protein expression of IL-1b and Panel B shows the corresponding mRNA data. The relative mRNA level was quantified by scanning densitometry and normalized to b-actin mRNA. Note the up-regulated protein and mRNA expression of IL-1b by LPS is suppressed by different concentrations of resveratrol; however, in cells pretreated with mTOR inhibitor rapamycin, the suppressive effect of resveratrol is abrogated. The values shown are mean 6 SEM of data from three independent experiments. #Significant compared with control alone, p,0.05. *Significant compared with LPS alone, p,0.05. nSignificant compared with resveratrol+LPS, p,0.05. doi:10.1371/journal.pone.0032195.g008 Figure 9. mTOR is required for resveratrol-inhibited expression of NF-kB/RelA protein and mRNA induced by LPS in BV-2 cells. Panel A shows the immunofluorenscence images for protein expression of NF-kB/RelA and Panel B shows the corresponding mRNA data. The relative mRNA level was quantified by scanning densitometry and normalized to b-actin mRNA. Note the up-regulated protein and mRNA expression of NF- kB/RelA by LPS is suppressed by different concentrations of resveratrol; however, in cells pretreated with mTOR inhibitor rapamycin, the suppressive effect of resveratrol is abrogated. The values shown are mean 6 SEM of data from three independent experiments. #Significant compared with control alone, p,0.05. *Significant compared with LPS alone, p,0.05. nSignificant compared with resveratrol+LPS, p,0.05. doi:10.1371/journal.pone.0032195.g009 PLoS ONE | www.plosone.org 7 February 2012 | Volume 7 | Issue 2 | e32195