Zhang et al. BMC Genomics 2011, 12:63 http://www.biomedcentral.com/1471-2164/12/63 Page 7 of 20 Figure 4 Comparison of unigenes expression between the mutant and the wild-type. The abundance of each gene was normalized as transcripts per million (TPM). The differentially expressed genes are shown in red and green, while blue indicates genes that were not differentially expressed genes (not DEGs) between the mutant and the wild-type. Table 2 Hit percentages against important public databasesa 36,523 Unigenes (All) 29,864 Unigenes (WT) Database SwissProt KEGG Nr COG Total aE value threshold is E-5. Annotated (n) 19,119 12,724 26,938 9,515 26,993 % 52.35 34.84 73.76 26.05 73.91 Annotated (n) 16,431 11,005 22,042 8,397 23,076 % 55.02 36.85 73.81 28.12 77.27 31,468 Unigenes (MT) Annotated (n) 16,675 11,279 23,770 8,446 23,835 % 52.99 35.84 75.54 26.84 75.74