BMC Genomics 2007, 8:139 http://www.biomedcentral.com/1471-2164/8/139 pF5ig3u-dreep4endent induction of target genes p53-dependent induction of target genes. Panels A-H show site-specific qPCR ChIP results on left with standard deviation, where significance at p ≤ 0.05 is designated by an asterisk (*). Center tile depicts gene map of predicted p53 binding site loca- tion and the sequence relative to annotated genes, along with exon structure and direction of transcription as per NCBI MapViewer conventions. Right tile represents RT-PCR gel image of each mapped target gene. RT-PCR analyses were per- formed multiple times for each target gene and gel images are representative of consistent expression levels. mRNA levels of each gene in wild-type naïve PC12 cells were compared to 7-day NGF (50 ng/mL) differentiated wild-type and three anti-p53 shRNA expressing PC12 cells (p53#3low, p53#2mid, p53#4mid). ImageJ quantitation, normalized to naïve RNA levels, is depicted under each band, while PCR cycle number is depicted on right of gel image. GAPDH expression levels are shown in A and represent a typical loading control along with no-RT negative control. Page 6 of 17 (page number not for citation purposes)