BMC Genomics 2009, 10:442 http://www.biomedcentral.com/1471-2164/10/442 RFPigSu1r9ee1xpression in patient 3 RPS19 expression in patient 3. a) RPS19 expression in patient 3 and controls is shown. Primers complementary to exon 1 (forward) and exon 4 (reverse) were used. Two PCR products are indicated in lane 1 at 628 and 557 bp. b) Sequencing of RPS19 in patient 3. Exon 2 is 71 nt long and the first three nucleotides represent the translation initiation start site (bold). The chromatogram shows deletion of exon 2 in c.1-1G>A allele compared to the wild-type sequence in patient 3. Page 4 of 12 (page number not for citation purposes)