A. Asian 2 p24.1 20863719 3 p25.2-25.3 9867502 6 p22.1 28165236 10 q22.3 77428562 10 q24.1 98845671 17 p13.1 7918593 17 q24.2 62769444 B. Caucasian 2 p23.3 23804032 3 p11.2-12.1 85893658 3 p14.2-14.3 56804094 3 p22.2-24.1 29648645 4 p15.2-15.3 21407638 6 p12.3 47804985 6 p22.1-22.2 25985490 6 q21.1 73325343 9 q22.2-22.3 76902029 10 q21.2 74105644 12 p13.3 8010296 12 q24.2 63460935 17 p22.2 15476489 X q21.3 87836131 aADC: adenocarcinoma; SCC: squamous cell carcinoma. 22199349 12488641 28452189 79105320 99207113 7993947 63340772 26257119 87649703 61897044 38540543 23952439 47961964 26318093 73596310 78697046 74697200 9309215 64329625 15931167 89896378 A. Asian 6 6 17 B. Caucasian 2 2 3 4 10 p21.2 p22.3 p13.3 p22.2-22.3 p25.1 p24.1-24.3 p15.2 q24.2-24.3 39548717 21096443 2443685 35262506 8929622 23113657 25955365 100717385 39877219 22252887 3461082 36832851 11112217 26730485 26477816 104630288 Lo et al. BMC Cancer 2012, 12:235 http://www.biomedcentral.com/1471-2407/12/235 Page 6 of 13 Table 2 Chromosome imbalance regions with different alteration frequency in relation to tumor subtypes in lung cancer ADCa SCCa Chromosome Cytoband Start site End site Size BAC Difference in (Mb) number (n=20) (n=20) alteration frequency from Asian and Caucasian databases with chromosome alteration frequency more than 50% were further ana- lyzed by MetaCore™ analysis software. Calculation of statistical significance throughout MetaCore for cellular pathways, disease-specific biomarker genes, and genes annotated in the gene ontology databases was based on P value, calculated using the hypergeometric distribution [14]. P value of 0.05 was used for the cutoff of candidate gene selection. Collectively, 214 genes were selected as NSCLC-related candidate genes, which are involved in cell cycle, apoptosis, cell migration, signal transduction, DNA repair and other cell growth and motility control Table 3 Chromosome imbalance regions with different alteration frequency in relation to tumor stages in lung cancer Chromosome Cytoband Start site End site Size BAC Early stage Late Stage Difference in (Mb) number (n=19) (n=21) alteration frequency Loss :85% Loss :25% Gain :85% Gain :15% Gain :50% Loss :10% Loss :60% Gain :60% Loss :100% Gain :20% Loss :70% 0 0 0 Loss :60% Loss :50% 0 Loss :90% Loss :10% Gain :60% 0 0 0 Gain :21% 0 Gain :14% Loss : 7% 0 0 Loss :30% Gain :40% Gain :25% Loss :45% Gain :70% Gain :45% 0 0 Loss :40% Gain :80% 0 Loss :80% Gain :60% Gain :50% 0 Gain :20% Gain :60% Loss :10% Loss :60% 0 Gain :60% Loss :62% Gain :66% Gain :71% Gain :100% Gain :100% Loss :60% Gain :60% Loss :100% 55% 65% 60% 60% 20% 55% 60% 60% 60% 60% 70% 80% 60% 50% 60% 70% 60% 80% 50% 60% 60% 62% 66% 50% 100% 86% 53% 60% 100% 1.30 2.60 0.30 1.70 0.36 0.10 0.60 2.45 1.76 5.09 8.89 2.54 0.16 0.33 0.27 1.80 0.59 1.30 0.87 0.45 2.06 0.32 1.15 1.01 1.57 2.18 3.62 0.52 3.91 10 18 6 17 4 2 5 17 14 33 25 27 2 5 2 17 6 11 14 4 17 13 39 9 11 16 32 4 44