Todorčević et al. BMC Genomics 2010, 11:39 http://www.biomedcentral.com/1471-2164/11/39 Page 6 of 17 Figure 5 Results of microarray analyses, selected genes with expression changes. Samples from days 1, 4, 7, 9, 15 and 30 (5 pooled flasks per day) were compared to an equalised mixture from all time-points. Data are log-ER (expression ratios). Significantly up- and down-regulated genes (p < 0.01, t-test, 12 spot replicates per gene) are highlighted with red and green scales. NS - not significant, ND - not detected. joining of ribosomal subunits resulting in the formation of a functional 80S initiation complex was up-regulated at the last time point (Fig. 5). The increase of several protein folding heat shock pro- teins and genes involved in protein degradation was evi- dent during this period (Fig. 5 and 10). The components of the 26S proteasome, ubiquitin and several enzymes involved in the ubiquitin conjugation to target proteins were up-regulated at day 15 and even more markedly at the end of the study period. High levels of 26S protea- some non-ATPase regulatory subunit 14 (POH1), were observed at day 30 while up-regulation of lysosomal proteases (cathepsins) was seen from day 15 (Fig. 5). Discussion Our transcriptomic analyses suggest, as expected, that early salmon aSVF culture contains a number of cell types, including vascular cells, macrophages and lym- phocytes, in addition to preadipocytes. Similar cell com- position of aSVF was reported in mammals [38]. The gene expression profiles at days 1 and 4 implied reten- tion of multipotency of aSVF cells during the early