Table 1 List of M. bovis BCG potential multiprotein complexes and proteins identified in this study proteina) description peptideb) SCc) pId) Mr scoref) TMHg) Gravyh) functioni) bandj) spotk) (kDa)e) Zheng et al. BMC Genomics 2011, 12:40 http://www.biomedcentral.com/1471-2164/12/40 Pks2 polyketide synthase MmpL8 integral membrane transport protein FadD23 fatty-acid-CoA ligase PapA1 polyketide synthase associated protein BCG_3890c putative transposase LpqW putative lipoprotein AtpA ATP synthase alpha chain AtpD ATP synthase beta chain AtpH ATP synthase delta chain LipO putative esterase AtpG ATP synthase gamma chain AtpB ATP synthase a chain AtpF ATP synthase b chain AtpC ATP synthase epsilon chain AtpE ATP synthase c chain PpsC phenolpthiocerol synthesis type-I polyketide synthase PpsA phenolpthiocerol synthesis type-I polyketide synthase EmbB integral membrane indolylacetylinositol arabinosyltransferase EmbC integral membrane indolylacetylinositol arabinosyltransferase EmbA integral membrane indolylacetylinositol arabinosyltransferase BCG_2759c putative membrane alanine rich protein Pks7 polyketide synthase Pks8 polyketide synthase DnaK chaperone protein GroEL2 60 KDA chaperonin 2 GroEL1 60 kDa chaperonin 1 DnaJ2 chaperone protein FurA ferric uptake regulation protein MmpL11 transmembrane transport protein BCG_0241c transmembrane protein BCG_0238c hypothetical protein RplB 50S ribosomal protein L2 RpsD 30S ribosomal protein S4 RpsE 30S ribosomal protein S5 RplE 50S ribosomal protein L5 RplV 50S ribosomal protein L22 RplM 50S ribosomal protein L13 RplR 50S ribosomal protein L18 SdhA succinate dehydrogenase (flavoprotein subunit) SdhB succinate dehydrogenase (iron-sulphur protein subunit) a) Protein name in M. bovis BCG database. b) Number of identified unique peptides obtained for a particular protein. c) Sequence coverage in percent of the identified protein. d) Isoelectric point of the protein that calculated from its amino acid sequence. e) Molecular weight in kDa of the respective protein. f) The Mascot score of the protein identification. g) Transmembrane helices predicted by TMHMM 2.0 program. h) Grand average of hydrophobicity predicted by ProtParam. i) Functional classification based on the Pasteur Institute functional classification tree. j) Number of BN gel bands for complex identification. k) Number of spots for protein identification. 10 3 4 4 2 3 14 19 9 4 9 2 6 5 2 8 12 4 7 3 4 12 6 11 5 6 3 2 3 3 2 9 2 3 5 8 2 6 20 4 4.09 5.18 4.78 9.85 8.56 5.42 11.35 5.33 3.19 11.94 4.25 5.23 22.60 4.89 46.50 4.71 18.40 5.28 36.07 10.59 21.30 5.26 12.80 6.14 39.80 5.11 47.10 4.55 49.40 4.78 5.30 4.92 7.25 2.55 6.31 3.56 5.02 10.14 10.42 10.12 12.22 12.05 8.09 5.29 4.50 5.21 26.72 4.70 11.30 4.70 9.46 4.84 10.21 6.24 15.33 5.46 2.48 10.14 6.32 10.29 8.98 7.75 29.30 11.94 9.50 10.33 13.20 10.74 19.30 10.52 44.20 12.24 17.00 10.41 46.70 12.10 36.78 5.65 12.55 8.93 227.13 116.28 62.84 56.13 46.11 66.37 59.48 53.18 48.83 46.10 33.93 27.51 18.32 13.13 8.06 231.22 199.69 118.14 117.83 115.92 29.06 221.75 167.47 66.79 56.69 55.84 41.03 16.53 104.06 45.89 18.52 30.62 23.46 22.93 21.01 20.37 16.34 13.18 65.24 30.01 60.02 35.23 29.57 27.47 31.02 104.53 1883.30 2917.93 285.95 36.07 593.00 80.91 115.66 43.53 24.67 59.56 132.56 40.51 59.83 39.95 98.48 154.53 41.55 202.48 82.72 61.98 31.23 23.78 34.28 20.18 44.45 100.45 95.47 24.67 73.79 162.57 22.52 133.98 1062.30 211.54 0 12 2 0 0 0 0 0 1 0 0 5 1 0 2 0 0 12 12 12 2 0 0 0 0 0 0 0 12 8 0 0 0 0 0 0 0 0 0 0 -0.001 0.330 0.064 0.013 -0.577 0.027 -0.207 -0.168 0.027 -0.113 -0.267 0.818 0.040 -0.061 1.016 0.028 -0.045 0.312 0.246 0.338 0.113 0.213 0.264 -0.368 -0.091 0.110 -0.237 0.013 0.337 0.323 -0.292 -0.851 -0.722 -0.171 -0.344 -0.494 -0.418 -0.329 -0.246 -0.154 1 3 1 1 5 3 7 7 7 7 7 7 7 7 7 1 1 3 3 3 10 1 1 0 0 0 0 9 3 3 10 2 2 2 2 2 2 2 7 7 Page 5 of 11 I I I I I II II II II II II II,IV II II II III III IV IV IV IV V V VI VI VI VI,II VI VII VII VII VIII VIII VIII VIII VIII VIII VIII IX IX 1 2 3 4 5 6 7 8 9 10 12 13,25 15 16 17 18 19 21 22 23 24 26 27 28 29 30 11,31 32 33 34 35 36 37 38 39 40 41 42 43 44