BMC Genomics 2006, 7:12 http://www.biomedcentral.com/1471-2164/7/12 PFhigyusirceal 5map of the VvF3'H locus Physical map of the VvF3'H locus. Map of BAC contig ctg253 carrying the VvF3'H-1 and VvF3'H-2 genes and its anchorage to LG17 of 'Cabernet Sauvignon' and V. vinifera consensus genetic maps. BAC clone 5A23 bears the VvF3'H-1 isogene and was included after PCR anchoring onto the contig using marker BES5A23-FM. Names written in horizontal refer to BAC clones and contigs; those written in vertical refer to markers and genes. The orientation of the contig is arbitrary. Genetic and physical distances are not drawn to scale. The presence of a given marker on a BAC clone is highlighted with the symbol '● '. The sym- bol 'X' stands for lack of amplification of a given marker in the corresponding BAC clone. BAC clones missing any symbol at marker BES5A23-FM were not tested. Physical localisation of VvF3'H-1 and VvF3'H-2 loci is shown in grey boxes. VvF3'5'H-2 was present in ctg871 with a SSCP haplotype not distinguishable from that found in ctg1320. Integration of genetic and physical maps around the locus VvF3'5'H Contigs ctg313, ctg871, ctg1320 and ctg2373 were anchored to the 'Cabernet Sauvignon' genetic map by using SSR markers present in the BAC clones and single strand conformational polymorphism of the BAC-end sequences (Figure 7). The SSR marker VrZag30 that was identified in five BACs of ctg313 allowed this contig to be anchored to LG6. Map position of ctg313 was confirmed by mapping the SSCP marker BES9C17-FM developed on a BAC clone embedded into ctg313 that co-segregated with SSR VrZag30. The contig ctg871 was anchored to LG6 by the SSR marker VMC3a8. The contig ctg1320 was anchored to LG6 using the SSR VMC3f12 and the BAC end-derived SSCP marker BES38C24-RM. The contig ctg2373 was anchored to LG6 by the BAC end-derived SSCP marker BES17K4-FM. The SSCP band of the marker Page 8 of 17 (page number not for citation purposes)