BMC Cancer 2005, 5:77 http://www.biomedcentral.com/1471-2407/5/77 Table 1: Clinical stage and HPV status of the analyzed samples. Additional material Sample Pre-malignant Invasive tumors G60 G67 G42 T56 T55 T46 T24 SXA LRL ASJ A69 VGR T49 CaLo ViBo INBL RoVa HeLa SiHa CasKi Stage CIN I CIN III CIN III Ib Ib Ib IIb IIb IIb IIb IIIb IIb IVa IIb IIb IVa IVa Cell lines HPV 58 16 31 16 16 16 16 16 16 16 18 18 18 18 18 16 16 Undetected Undetected Undetected possibility to combine LOH with copy number changes, might be useful for the detection of gene specific targets that are relevant for the genesis and progression of cervical carcinoma. Abbreviations CGH: Comparative Genomic Hybridization, UCC: Uter- ine cervix carcinoma, HPV: Human papilloma virus, DAPI: 4,6-diamino-2-phenylindole. Competing interests The author(s) declare that they do not have any compet- ing interests. Authors' contributions AH: Performed the microarray CGH experiments, data analysis and paper writing; MB: Help with data submis- sion to the Progenetix database, data analysis; IP: pro- vided training for the experiments, CGH data analysis; PP: tissue processing; GV: HPV typing; DH: sample collection; JG: provided access to the samples; ML: access to samples, histopathological analysis; RL: sample collection; CP: DNA extraction; JG: Help with data analysis; KV: DNA extraction; BA: HPV typing; MS: project coordinator. Additional File 1 Raw data from the CGH microarray experiments. This is text file contain- ing the raw data from the microarrays, the first column denotes the iden- tification of the clone, column 2 represents the cytogenetic position of the clone, sample data begins in column 3. Click here for file [http://www.biomedcentral.com/content/supplementary/1471- 2407-5-77-S1.txt] Acknowledgements This work was partially funded through the 7114 and 34686 grants from the Mexican Council of Science and technology (CONACyT) and the Mexican Institute for Social Security (IMSS-FOFOI FP- 2001–2003). AH, GVO, CP, RL were recipients of scholarships from the CONACyT, IMSS and DGEP- UNAM. We would like to thank Abbott-Vysis for providing the CGH array system for this analysis. This work was submitted in partial fulfillment of the requirements for the Ph.D. degree of HA at the Ph.D. in Biomedical Sci- ences, National University of Mexico. References 1. Walboomers J, Jacobs M, Manos M, Bosch X, Kummer A, Shah K, Snijders P, Peto J, Meijer C, Muñoz N: Human Papilloma Virus is a necessary cause of invasive cervical cancer worldwide. J Pathol 1999, 189:12-19. 2. Bosch X, Muñoz N: The viral etiology of cervical cancer. Virus Res 2002, 89:183-190. 3. Münger K, Howley P: Human papillomavirus immortalization and transforming functions. Virus Res 2002, 89:213-228. 4. Ried T, Hesselmeyer K, Blegen H, Schröck E, Auer G: Genomic changes defining the genesis, progression and malignancy in solid human tumors: a phenotype/genotype correlation. Genes Chrom Cancer 1999, 25:195-204. 5. Pihan G, Wallace J, Zhou Y, Doxsey S: Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas. Cancer Res 2003, 63:1398-1404. 6. Cahill DP, Kinzler KW, Vogelstein B, Lengauer C: Genetic instabil- ity and darwinian selection in tumours. Trends Cell Biol 1999, 9:M57-M60. 7. Heselmeyer K, Schröck E, Du Manoir S, Blegen H, Shah K, Steinbeck R, Auer G, Ried T: Gain of chromosome 3q defines the transi- tion from severe dysplasia to invasive carcinoma of the uter- ine cervix. Proc Natl Acad Sci USA 1996, 93:479-484. 8. Heselmeyer K, Macville M, Schröck E, Blegen H, Hellström A, Shah K, Auer G, Ried T: Advanced stage cervical carcinomas are defined by a recurrent pattern of chromosomal aberrations revealing high genetic instability and a consistent gain of chromosome arm 3q. Genes Chrom Cancer 1997, 19:233-240. 9. Dellas A, Torhorst J, Jiang F, Proffit J, Schultheiss E, Holzgreve W, Sau- ter G, Mihatsch M, Moch H: Prognostic value of genomic alter- ations in invasive cervical squamous cell carcinoma stage IB detected by comparative genomic hybridization. Cancer Res 1999, 59:3475-3479. 10. Kirchhoff M, Rose H, Petersen B, Maahr J, Gerdes T, Lundsteen C, Bryndorf T, Kryger-Baggesen N, Christensen L, Engelholm S, Philip J: Comparative genomic hybridization reveals a recurrent pat- tern of chromosomal aberrations in severe dysplasia/carci- noma in situ of the cervix and in advanced-stage cervical carcinoma. Genes Chrom Cancer 1999, 24:144-150. 11. Allen D, White D, Hutchins A, Scurry J, Tabrizi S, Garland S, Armes J: Progressive genetic aberrations detected by comparative genomic hybridization in squamous cell carvical cancer. Br J Cancer 2000, 83:1659-1663. 12. Kirchhoff M, Rose H, Petersen B, Maahr J, Gerdes T, Philip J, Lunds- teen C: Comparative genomic hybridization reveals non-ran- Page 6 of 7 (page number not for citation purposes)