BMC Genomics 2008, 9:298 http://www.biomedcentral.com/1471-2164/9/298 Table 2: Expression changes accompanying environmental challenge in T. brucei. Experiment SMB grown with 0%FBS 5 mg/ml BSA vs with 10% FBS SMB grown with 0%FBS, 5 mg/ml BSA, 0.3 mg/ mg Tfn vs with 10% FBS SMB grown with 30%FBS vs with 10% FBS SMB grown for 1 hr with 1 mM DTT vs without SMB grown for 4 hr with 1 mM DTT vs without SMB grown for 4 hr with 5 ug/ml Tunicamycin vs without SMB grown for 24 hr with 5 ug/ml Tunicamycin vs without VSG RNAi induced vs uninduced Day1 Gene Gp63 (TbMSP-C) procyclin procyclin cysteine peptidase ESAG6 ESAG7 14-3-3-like Gp63 (TbMSP-C) procyclin cysteine peptidase ESAG6 ESAG7 ISG65-like CAMK4 kinase procyclin GTP1/OBG/Hsr1 putative ABC transporter ISG65-like ESAG7 MAP kinase Rab3GAP dual-specificity protein phosphatase TbGRP hypothetical ER chaperone Ubiquitin conjugating Vta1-like syntaxin Hsp60 chaperonin GMP-PDE delta Rab11a Ser peptidase (Bem46-like) Sec24 procyclin ESAG6 procyclin AP2-kinase1 procyclin procyclin ESAG7 ESAG6 RabGAP GOLD domain PDI/ERp72 RabGEF/Vps9 Accession Tb10.70.5290 Tb10.6k15.0030 Tb927.6.450 Tb927.6.960 H25N7.15 N19B2.155 Tb11.02.4700 Tb10.70.5290 Tb10.6k15.0030 Tb927.6.960 H25N7.15 N19B2.155 Tb927.5.410 Tb927.4.3770 Tb10.6k15.0030 Tb927.2.5060 Tb10.329.0040 Tb927.5.410 N19B2.155 Tb10.70.2070 Tb10.6k15.1390 Tb927.5.3620 Tb10.6k15.1520 Tb11.01.8120 Tb927.7.6960 Tb927.7.7140 Tb10.61.1980 Tb10.70.0280 Tb927.2.4580 Tb927.8.4330 Tb09.211.2310 Tb927.3.5420 Tb10.6k15.0030 H25N7.15 Tb927.6.450 Tb09.160.4770 Tb10.6k15.0030 Tb927.6.450 N19B2.155 H25N7.15 Tb10.6k15.1790 Tb927.7.3600 Tb10.6k15.2290 Tb927.3.2430 Fold up (down) vs control qRT-PCR array 2.9 2.4 2.0 1.9 1.8 1.6 (1.9) 3.1 2.2 2.0 2.0 1.4 (2.9) (2.2) (1.9) 2.4 2.2 (2.9) (2.2) (2.1) (2.1) (1.9) 3.6 3.0 2.8 2.7 2.7 (3.0) (2.8) (2.8) (2.4) (2.4) 2.4 1.9 1.7 (2.0) 4.2 2.7 2.6 2.2 2.1 2.1 (2.3) 2.1 2.2 2.4 2.3 3.6 3.9 4.7 1.5 1.9 4.6 4.1 3.5 (4.1) (3.6) (1.9) (2.0) 3.3 (1.8) 2.1 2.3 2.3 7.8 8.8 4.3 2.8 Page 14 of 28 (page number not for citation purposes)