BMC Cancer 2008, 8:19 http://www.biomedcentral.com/1471-2407/8/19 iFGnieganumerseicidr5oenartirfaieydstinudtyheonSSmHeliabnroarmyadpisrtoinggrueissshionnon-neoplastic from neoplastic tissues and primary from metastatic melanomas Genes identified in the SSH library distinguish non-neoplastic from neoplastic tissues and primary from meta- static melanomas in a microarray study on melanoma progression. Expression data from the microarray analysis by Haqq et al [16] were collected for the genes identified in the RGP and Met libraries. The expression data for each gene group were submitted to SAM (FDR = 0) in a two-class analysis for detection of genes differentially expressed between primary and metastatic tumors and between non-neoplastic (skin and melanocytic nevi) and neoplastic (primary and metastatic melanomas) samples. The results from SAM analysis were extracted using SAMTERS and visualized by CLUSTER 3.0 and Java TreeView – Red and green squares represent genes up-regulated and down-regulated, respectively. (A) Expression profiles from primary and metastatic tumors for genes from the RGP library. (B – C) Expression profiles from non-neoplastic and neoplastic samples for genes from the RGP (B) and Met (C) libraries. Vertical blue lines on the left side indicate: (B) Genes from the RGP library that showed up-regulation in non-neoplastic samples; and (C) Genes from the Met library up-regulated in neoplastic tissues. Page 12 of 18 (page number not for citation purposes)