Natarajan et al. BMC Genomics 2010, 11:606 http://www.biomedcentral.com/1471-2164/11/606 62. Ewing B, Hillier L, Wendl MC, Green P: Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 1998, 8:175-185. 63. Huang X, Madan A: CAP3: A DNA sequence assembly program. Genome Res 1999, 9:868-877. 64. ClustalW2 Program. [http://www.ebi.ac.uk/Tools/clustalw2/index.html]. 65. Open Reading Frame (ORF) Finder. [http://www.ncbi.nlm.nih.gov/projects/ gorf]. 66. The Arabidopsis Information Resource. [http://www.arabidopsis.org]. doi:10.1186/1471-2164-11-606 Cite this article as: Natarajan et al.: Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds. BMC Genomics 2010 11:606. Page 14 of 14 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit