CG31637 CG3689 CG8475 didum Ggamma1 CG1441 Rh6 CG31163A CG18854 CG7908 CG14507 CG33085A,B CG32316B CG32912B CG33047A,B CG33075B CG32956B CG33038B CG32791 tinc CG9932 CG30389 CG3975 CG8963 CG3638 CG33006B CG31305B CG32206A CG12071 3 3 3 3 3 3 4 3 mir-92b mir-210 mir-263b mir-276a-3 3 mir-277 3 mir-278 mir-278 mir-289A mir-306-3 mir-309 mir-276a-5 3 mir-276b-5 3 mir-184-3 3 mir-284A 3 mir-184-3 3 mir-278 3 mir-279 3 mir-133 3 mir-284A 3 mir-306-3 3 mir-9a 5 mir-9b 5 mir-9c 5 mir-33 3 mir-9a 4 mir-9b 5 mir-9c 4 mir-31a 3 mir-34 3 mir-263b 4 mir-14 3 mir-34 3 mir-33 3 mir-6 3 mir-184-3 3 mir-278 3 mir-278 4 mir-289A 3 mir-305 3 -17, -18, -17 -20, -15, -17 -18, -17, -21 -18, -16, -15 -19, -16, -15 -30, -22, -26 -19, -22, -20 -17, -15, -17 -27, -19, -24, -21 -17, -19, -22 -24, -20, -21 -24, -20, -21 -21, -22, -16 -23, -25, -17 -20, -22, -16 -18, -20, -23 -15, -21, -16 -19, -17, -19 -27, -17, -15 -26, -20, -27 -17, -19, -18, -21, -18 -17, -20, -16, -20, -17 -17, -22, -18, -20, -18 -19, -23, -18 -20, -17, -16, -18 -18, -19, -17, -16, -16 -21, -17, -17, -19 -15, -17, -15 -22, -23, -33 -16, -21, -20, -21 -17, -16, -25 -28, -21, -24 -17, -19, -22 -16, -17, -17 -27, -17, -22 -20, -15, -23 -16, -32, -29, -25 -15, -15, -16 -17, -21, -19 carrier activity multiple multiple unknown unknown unknown unknown unknown unknown unknown unknown multiple unknown unknown BMC Genomics 2005, 6:88 http://www.biomedcentral.com/1471-2164/6/88 Table 1: microRNA/target pairs predicted by MovingTargets using the following strict targeting criteria: minimum 3 target sites, maximum dG of microRNA/target hybridization of -15 kcal/mole at RT for each target site, minimum 7 out of 8 consecutive base pairs in microRNA 5' end, maximum 1 G:U base pair in microRNA 5' region. Transcription factor targets are listed first followed by neural targets. Gene function and biological process are as given by FlyBase http://www.flybase.net, March 2004. (Continued) A: exact 5' and 3' ends for mir-263a, mir-274, mir-280, mir-282, mir-284, mir-289 have not yet been determined (6); target predictions for these microRNAs are likely to change once the precise microRNA sequences are known B: gene annotated as a "gene cassette"; molecular function and biological process listed is the union of molecular functions and biological processes for each ORF in the putative dicistronic transcript measurement of the effect of the miRNA. When let-7 is coexpressed with the luc/ab reporter, the level of luciferase activity is substantially reduced (Fig. 1B). To confirm that expression of the luciferase reporter in this system is not simply sensitive to the coexpresssion of any miRNA, the experiment was also performed using miR-92b instead of let-7. Using the parameters noted above, MovingTargets does not predict mir-92b to target ab. Exogenous miR-92b sulfotransferase activity carbohydrate metabolism pre-mRNA splicing factor activity mRNA cleavage, nuclear mRNA splicing, via spliceosome kinase activator activity, glycogen metabolism phosphorylase kinase regulator activity multiple multiple heterotrimeric G-protein G-protein coupled receptor protein signaling GTPase activity pathway oxidoreductase activity unknown G-protein coupled phototransduction, visual perception, sensory photoreceptor activity perception SH3/SH2 adaptor protein activity unknown inositol-triphosphate 3-kinase unknown activity zinc ion binding, cell surface receptor linked signal metalloendopeptidase avtivity transduction, proteolysis and petidolysis phospholipase A2 activity unknown argininosuccinate lyase activity unknown oxoglutarate dehydrogenase tricarboxylic acid cycle (succinyl-transferring) activity peptidoglycan recognition activity immune response alpha-L-fucosidase activity O-glycoside catabolism, fucose metabolism transport multiple multiple unknown unknown unknown unknown unknown unknown unknown unknown unknown unknown heparan sulfate proteoglycan biosynthesis, proton transport Page 6 of 11 (page number not for citation purposes)