BMC Cancer 2005, 5:104 http://www.biomedcentral.com/1471-2407/5/104 tEFyxipgpeurerIIsescie3olnlpohfeGn oIRtyKpech(aAn5n4e9ls) ianndadceanroccinaorcidin(oHm7a27a)lvceeollalrines Expression of GIRK channels in adenocarcinoma alveolar type II cell phenotype (A549) and carcinoid (H727) cell lines. Top panel: GIRK1 was expressed in both A549 and H727 cell lines. Bottom panel: Both A549 and H727 expressed GIRK2 and GIRK4. Cyclophilin, used as a positive reaction control was seen in both samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; GIRK2-438 bp; GIRK4-401 bp; cyclophilin- 216 bp; M-100 bp. nFDoiigfmfuearreecne4cllelsiniensGIRK expression in three squamous carci- Differences in GIRK expression in three squamous carcinoma cell lines. Top panel: GIRK1 was expressed in all three squamous cell lines, H2170, H226, and H520. Middle panel: GIRK2 is only expressed in H520 cell line, but not in H2170 or H226. GIRK4 was expressed in all three squamous cell lines, H2170, H226, and H520. Bottom panel: Cyclophi- lin, used as a positive reaction control was seen in all sam- ples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; GIRK2-438 bp; GIRK4-401 bp; cyclo- philin-216 bp; M-100 bp. Page 5 of 10 (page number not for citation purposes)