Zhang et al. BMC Genomics 2011, 12:63 http://www.biomedcentral.com/1471-2164/12/63 Page 14 of 20 Figure 7 Real-time quantitative RT-PCR confirmation of the differentially expressed genes between the mutant (black columns) and the wild-type (gray columns). The transcript abundance from MPSS data is shown above each gene; TPM, transcripts per million. Relative transcript levels are calculated by real-time PCR with b-actin as the standard. Data are means ± SE of three separate measurements. For comprehensive identification of candidate genes in the citrus flowering pathways, we were able to identify 110 homolog genes among about 36,523 unigene sequences, representing putative citrus homologs to flowering-time genes (Table 3, Additional file 7). Sequences found in citrus corresponded to all known Arabidopsis flowering-time pathways, suggesting that all of these genetic pathways may be present in citrus.