BMC Genomics 2005, 6:125 http://www.biomedcentral.com/1471-2164/6/125 A. Homo sapiens (Human) tcccgaccagtcctgaccgcacgggggccgcggccacggggcgcaggggccatggtgcgc M V R ggcaggatctcccggctctcggtccgggacgtgcgcttccccacgtcgcttgggggccac G R I S R L S V R D V R F P T S L G G H ggcgcggacgccatggtaagcg..(6kb intron)..ctcctgcagcacacggaccct.. G A D A M H T D P .. B. Anopheles gambiae (mosquito) ctcagcagcatggggaaggatcggtgcctcaacattaccaccctgcaggcaaaggatatc M G K D R C L N I T T L Q A K D I Cgatggccaacgtcactgggagcgcacgggtccgatgcgatggtatgtgtgcgtcgaa.. R W P T S L G A H G S D A M ..(76 bp intron)..tccgatcaaagtagcacacggatccggactattcctgcgt.. H T D P D Y S C V .. C. Ustilago maydis (fungi, Basidiomycetes) ttgattgacatcaaattactaagtaccgccttgcctaccatgaccaagttatcgccgctg M T K L S P L gtcgtctcgtcgtattcgatacacgatgtacgctttcccacttcgctcaccggagatggc V V S S Y S I H D V R F P T S L T G D G Acggacgctatgaacaagtgtaagtgtagtaagtgtacaagccaccgttaatgagcag.. T D A M N K D. Magnaporthe grisea (fungi, Ascomycetes) ttctctgtccagctgtttatccttttgacttattttcttataatcttctggtcaaaaata atggcagctcaagaaatcaccattacgggctggaaaacccgcgatgtccggttccccaca M A A Q E I T I T G W K T R D V R F P T tctttggacaagacggggtcggatgccatgaacgctgcgggtgattactcgtcagcctat S L D K T G S D A M N A A G D Y S S A Y tgcatccttgagacagactcagagttctcaggtcatggaatggttagtagtggcatatat C I L E T D S E F S G H G M E. Sinorhizobium meliloti ( -proteobacteria) cgctgcaggtctattggagcaaacatgacccgcatcaccgaccttcgcgtcttcgacctt M T R I T D L R V F D L cgctttccgacctcggcgagcctcgacggttcggacgccatgaacccggatccggactat R F P T S A S L D G S D A M N P D P D Y PFrigeduircetio1n of extended N-termini for rTS genes Prediction of extended N-termini for rTS genes. The genomic sequences and the predicted protein translations for the N-terminal regions from four different species, Homo sapiens, Anopheles gambiae, Ustilago maydis, and Sinorhizobium meliloti, are shown in panels A to D, respectively. A double underlined "ATG" indicates the predicted start codon for the extended N-ter- minus, while the "ATG" with single underline indicates the start codon of the isoform with a shorter N-terminus. Grey high- lights indicate the canonical "GT....AG" intron motif. Page 3 of 10 (page number not for citation purposes)