Arias et al. BMC Genomics 2011, 12:390 http://www.biomedcentral.com/1471-2164/12/390 Page 4 of 17 Table 1 S. cerevisiae mutants with increased levels of dually phosphorylated MAP Kinase Slt2 ORF Gene P-Slt2 Functional Group Description YAL058W CNE1 16.4 Cell wall and morphogenesis Calnexin 1. may be involved in a QC process for secretory pathway proteins YBL007C SLA1 8.1 Cell wall and morphogenesis Functions in the assembly of cortical actin cytoskeleton YBR078W ECM33 14.4 Cell wall and morphogenesis GPI-anchored protein required for CW integrity and mannoprotein biosynthesis YCR088W ABP1 2.3 Cell wall and morphogenesis Actin binding protein that functions in clathrin- and actin-mediated endocytosis YDL095W PMT1 10.8 Cell wall and morphogenesis Mannosyltransferase, first step in O-glycosylation YDR349C YPS7 16.6 Cell wall and morphogenesis GPI-anchored aspartyl protease involved in maintaining cell wall integrity YJL062W LAS21 3.6 Cell wall and morphogenesis Protein required for addition of a side chain to the GPI core structure YJL099W CHS6 2.9 Cell wall and morphogenesis Protein involved in chitin synthase Chs3p activity YJR075W HOC1 2.1 Cell wall and morphogenesis Alpha-1,6-mannosyltransferase activity YLR319C BUD6 2.4 Cell wall and morphogenesis Budding 6, required for bipolar budding and involved in bud site selection YLR337C VRP1 8.6 Cell wall and morphogenesis Proline-rich protein verprolin, involved in cytoskeletal organization YLR350W ORM2 2.6 Cell wall and morphogenesis Putative transmembrane protein that may function in the CWI pathway YLR370C ARC18 5.5 Cell wall and morphogenesis Component of the ARP2/3 actin-organizing complex, involved in actin assembly YMR307W GAS1 27.4 Cell wall and morphogenesis GPI-anchored protein with 1,3-beta-glucanosyltransferase activity YNL079C TPM1 7.2 Cell wall and morphogenesis Tropomyosin, functions in a variety of processes involving the actin cytoskeleton YNL116W DMA2 3.4 Cell wall and morphogenesis Functions in spindle positioning and septin ring assembly YNL192W CHS1 2.0 Cell wall and morphogenesis Chitin synthase I, has a repair function during cell separation YOR002W ALG6 6.3 Cell wall and morphogenesis Dolichyl-phosphate-mannose-protein mannosyltransferase activity YER037W PHM8 4.0 Metabolism Lysophosphatidic acid phosphatase YJL137C GLG2 2.3 Metabolism Transferase activity, transferring hexosyl groups YHL023C RMD11 2.9 Nuclear Protein possibly involved in meiotic nuclear division YPR031W NTO1 2.0 Nuclear May be involved in chromatin-mediated transcription regulation YBR101C FES1 2.8 Protein metabolism NEF for the cytosolic chaperone Ssa1p. Response to oxidative stress YDR069C DOA4 2.6 Protein metabolism Ubiquitin-specific protease that acts in recycling ubiquitin YFL007W BLM10 4.0 Protein metabolism Proteasome assembly YBL024W NCL1 5.8 RNA metabolism tRNA (cytosine-5-)-methyltransferase activity YPL029W SUV3 2.0 RNA metabolism Mitochondrial RNA helicase of the DEAD box family YPL213W LEA1 9.0 RNA metabolism RNA splicing factor activity YDL047W SIT4 4.3 Signal transduction Serine/threonine phosphatase involved in cell cycle regulation/ion homeostasis YDR162C NBP2 16.9 Signal transduction Negative regulator of the HOG pathway YDR389W SAC7 7.5 Signal transduction GTPase-activating protein for Rho1p YER155C BEM2 2.4 Signal transduction GTPase-activating (GAP) protein that regulates Rho1p YHR082C KSP1 2.9 Signal transduction Serine/threonine kinase involved in filamentous growth YHR206W SKN7 2.5 Signal transduction Transcription factor involved in the oxidative and osmotic stress responses YJR074W MOG1 5.4 Signal transduction Involved in nuclear protein import, plays a role in osmoregulation via SLN1-SKN7 YKL126W YPK1 2.3 Signal transduction Putative S/T protein kinase possibly related with the CWI and sphingolipids YLR371W ROM2 2.5 Signal transduction GDP-GTP exchange factor for Rho1p YNL053W MSG5 5.4 Signal transduction Dual-specificity protein tyrosine phosphatase involved in response to pheromone YNR047W FPK1 3.9 Signal transduction Serine/threonine protein kinase that regulates phospholipid asymmetry YLR242C ARV1 8.6 Sphingolipid metabolism Protein involved in sterol uptake required for normal sphingolipid metabolism YER139C RTR1 5.2 Transcription Protein required for growth at high temperature, RNA polymerase II factor YMR136W GAT2 10.7 Transcription GATA zinc finger transcription factor YNL025C SSN8 2.2 Transcription RNA polymerase II transcription mediator YPL042C SSN3 3.9 Transcription Cyclin-dependent serine/threonine protein kinase of the RNA polymerase II YPR065W ROX1 4.7 Transcription Transcriptional repressor of hypoxic genes YPR115W 3.6 Transcription Phosphoinositide binding. Response to oxidative stress YDR200C VPS64 3.9 Transport Class B vacuolar sorting protein involved in Prc1p trafficking/a-factor secretion YFL048C EMP47 4.1 Transport Golgi and ER membrane protein involved in glycoprotein secretion YGR028W MSP1 2.1 Transport Intra-mitochondrial sorting protein, member of the AAA family of ATPases YHR032W ERC1 3.5 Transport Member of the MatE family. Transporter activity YJL133W MRS3 5.2 Transport Mitochondrial carrier (MCF, family of membrane transporters). Iron transport