Sarmiento-Rubiano et al. BMC Genomics 2010, 11:504 http://www.biomedcentral.com/1471-2164/11/504 Table 4 DNA re-association values as determined by DNA macroarray analysis among Lactobacillus strains L. intestinalis DSMZ 6629T L. johnsonii ATCC 33200T L. crispatus DSMZ 20584T L. gasseri ATCC 33323T L. acidophilus CECT 903T L. murinus BL262 L. reuteri BL259 L. intestinalis DSMZ 6629T L. intestinalis BL260 L. johnsonii ATCC 33200T L. johnsonii CECT 289 L. johnsonii NCC 2822 L. johnsonii NCC 1741 L. johnsonii NCC 533 L. johnsonii NCC 2767 L. johnsonii BL261 L. crispatus DSMZ 20584T L. crispatus BL221 L. gasseri ATCC 33323T L. gasseri NCC 2856 L. gasseri NCC 2857 L. gasseri NCC 2858 L. gasseri BL223 L. acidophilus CECT 903T L. acidophilus CECT 4529 L. acidophilus CECT 4179 L. taiwanensis BL263 L. taiwanensis BL301 L. taiwanensis BL302 L. taiwanensis BL303 L. taiwanensis BL304 1.95 ± 1.39 4.33 ± 4.16 100.00 107.55 ± 4.18 7.74 ± 1.49 10.44 ± 3.74 15.39 ± 8.09 17.57 ± 7.53 9.70 ± 5.52 16.00 ± 7.28 7.07 ± 3.58 7.34 ± 2.86 7.14 ± 2.66 3.99 ± 1.88 10.56 ± 5.92 11.54 ± 6.36 8.07 ± 3.82 5.06 ± 2.07 8.28 ± 2.65 8.59 ± 3.38 7.10 ± 3.61 7.50 ± 1.18 3.55 ± 0.51 3.57 ± 1.55 3.55 ± 0.29 3.40 ± 0.25 3.68 ± 2.29 6.04 ± 4.61 6.00 ± 1.26 9.90 ± 3.70 100.00 81.91 ± 8.39 94.25 ± 12.15 91.82 ± 8.88 76.78 ± 8.09 106.05 ± 7.91 56.49 ± 8.70 6.22 ± 1.06 6.84 ± 0.86 15.62 ± 2.19 31.63 ± 9.46 41.76 ± 12.27 29.36 ± 9.15 21.28 ± 1.75 7.06 ± 0.08 6.79 ± 1.73 5.12 ± 1.50 31.43 ± 6.28 18.20 ± 5.85 19.56 ± 4.20 17.65 ± 6.63 17.31 ± 6.16 82.06 ± 10.06 3.63 ± 1.61 2.41 ± 0.83 3.87 ± 4.17 3.66 ± 1.10 7.75 ± 4.33 5.15 ± 0.70 5.45 ± 2.02 1.39 ± 6.29 10.48 ± 6.32 9.73 ± 7.20 13.33 ± 8.54 6.04 ± 2.44 100.00 7.73 ± 3.08 11.11 ± 6.48 5.98 ± 3.43 4.21 ± 1.38 9.84 ± 3.14 9.08 ± 3.40 6.72 ± 2.72 6.98 ± 2.42 3.82 ± 0.34 3.94 ± 0.88 3.74 ± 1.00 3.47 ± 0.75 4.82 ± 2.47 8.12 ± 4.71 4.08 ± 0.86 9.93 ± 4.83 19.32 ± 1.51 23.12 ± 7.98 45.64 ± 4.48 38.06 ± 8.11 24.53 ± 8.96 45.02 ± 14.95 19.74 ± 3.73 4.28 ± 2.06 4.88 ± 2.97 100.00 76.31 ± 6.36 79.66 ± 15.14 71.09 ± 5.46 62.11 ± 9.80 6.18 ± 2.42 3.68 ± 0.61 3.39 ± 1.61 22.05 ± 3.10 12.61 ± 2.42 13.18 ± 0.71 12.96 ± 2.37 12.73 ± 2.10 1.23 ± 0.07 5.93 ± 2.16 6.79 ± 2.11 9.77 ± 0.23 7.57 ± 1.73 8.61 ± 3.24 13.81 ± 6.55 10.39 ± 3.73 8.31 ± 3.82 11.35 ± 3.62 12.02 ± 10.84 11.70 ± 5.35 8.40 ± 2.87 3.25 ± 0.77 6.57 ± 2.36 8.73 ± 3.21 8.92 ± 4.47 3.29 ± 0.88 100.00 98.60 ± 6.52 101.10 ± 2.45 6.19 ± 1.73 3.26 ± 0.66 2.56 ± 0.89 3.23 ± 0.05 3.57 ± 0.64 Page 11 of 16 L. taiwanensis BL263 15.22 ± 4.65 4.78 ± 2.07 3.52 ± 1.05 7.27 ± 1.86 20.76 ± 1.32 28.28 ± 6.53 35.37 ± 11.93 35.18 ± 9.31 24.94 ± 8.71 39.75 ± 11.45 24.15 ± 2.27 4.20 ± 1.90 4.21 ± 1.34 9.89 ± 2.56 21.74 ± 6.62 25.55 ± 8.95 19.56 ± 5.14 14.13 ± 2.67 4.74 ± 0.80 3.96 ± 1.23 3.96 ± 0.64 100.00 72.61 ± 10.45 94.95 ± 5.69 86.54 ± 8.38 75.04 ± 8.82 Values were calculated as percentage of hybridization relative taking as a 100% the hybridization of each strain with itself and they are the means ± SD from three independent hybridization experiments. Intra-specific DNA hybridization values are shown in bold. the genomic level (> 72%). They were therefore regarded to belong to the same species. During the preparation of this manuscript a new L. taiwanensis species isolated from silage was described [39]. 16S rRNA encoding gene and multilocus sequence analyses showed that our strains isolated from rat intestine belong to L. taiwanen- sis species. This species is placed within the L. acidophi- lus group, but they constituted a different cluster from their closest relatives, L. johnsonii and L. gasseri. The five L. taiwanensis strains have been isolated from the same environment and showed very high homology in the phylogenetic analyses; however, the carbohydrate utilization and growth profiles clearly distinguish L. tai- wanensis BL301 from the groups made by L. taiwanensis strains BL304 and BL302 and by L. taiwanensis strains BL303 and BL263 (Table 3 and Fig. 1A). These two cou- ples of strains can be distinguished by their protein pro- files (Fig. 1B) and by DNA-DNA hybridization