Won et al. BMC Cancer 2010, 10:564 http://www.biomedcentral.com/1471-2407/10/564 Page 3 of 8 Figure 1 Cyclin D1, survivin, ERCC1, and TP expressions by immunohistochemistry in biliary tract cancer (magnification ×200). BDC was more common in men (p = 0.001). There was no significant difference in age, stage and resectability between GB cancer and BDC. Among 161 patients, 127 patients experienced surgical resection with curative intent, and 70 patients (55.1%) took adjuvant therapy with chemotherapy or chemo-radiation based on 5-FU. Immunohistochemical staining and correlation with clinico-pathological variables Histopathology showed that there was no significant dif- ference in differentiation, vascular invasion, and lympha- tic invasion between GB cancer and BDC. However, BDC showed significantly higher perineural invasion compared to GB cancer (p = 0.001) (Table 1). The immunohistochemical staining results are listed in Table 2. Immunohistochemistry results of some tissue cores cannot be determined, because of inadequate or incor- rect expression and loss of tissue samples in the tissue microarray. Among all cases on which interpretation of immunohistochemistry was possible, 72 cases (56.7%) and 35 cases (26.7%) showed positive expression for p53 and cyclin D1, respectively, 54 cases (41.9%) for survivin, and 78 cases (65.0%) for TP. However, ERCC1 expres- sion was mostly negative in 74 cases (65.5%). In com- parison, TP expression was significantly higher in GB cancer than in BDC (p = 0.006). We analyzed the corre- lations between the clinico-pathololgical findings and the expression of these proteins (Table 3). For all