BMC Genomics 2005, 6:88 http://www.biomedcentral.com/1471-2164/6/88 Table 1: microRNA/target pairs predicted by MovingTargets using the following strict targeting criteria: minimum 3 target sites, maximum dG of microRNA/target hybridization of -15 kcal/mole at RT for each target site, minimum 7 out of 8 consecutive base pairs in microRNA 5' end, maximum 1 G:U base pair in microRNA 5' region. Transcription factor targets are listed first followed by neural targets. Gene function and biological process are as given by FlyBase http://www.flybase.net, March 2004. # target dG of miRNA-mRNA sites hybrid (kcal/mole) Molecular Function Biological process Target CrebA fkh Eip74EF Eip93FA pros zfh1 zfh2 SoxN HLHm5 CG32527B ab sbb Syn nerfin-1 synaptogyrin Pkc98E nAcRbeta-96A RhoGEF2 WA kel adat bru-2 fus mblA Asph CG32062A CG32429 lmg wb Cbl Pdi mir-276a-3 3 mir-34 3 mir-309 3 mir-7 3 mir-34 3 miRNA mir-92b mir-312 mir-315 mir-34 mir-280A mir-34 mir-5 let-7 mir-33 mir-279 mir-286 mir-92b mir-313 mir-210 mir-210 bantam mir-280A mir-310 mir-311 mir-312 mir-9c mir-3 mir-309 mir-318 mir-9a mir-9c mir-12 mir-280A mir-303 mir-280A mir-9b mir-33 mir-34 mir-34 mir-34 mir-34 mir-263b mir-305 mir-316 3 4 3 3 3 3 3 5 3 3 3 3 3 3 3 4 3 3 4 4 3 3 3 3 3 3 4 3 3 3 3 3 3 4 3 4 3 4 3 -16, -24, -19 -16, -20, -17, -22 -18, -16, -17 -25, -27, -21 -21, -15, -16 -18, -17, -18 -17, -15, -15 -16, -15, -17 -23, -24, -16 -19, -16, -20 -24, -24, -26 -25, -24, -21 -16, -17, -19 -19, -17, -24 -19, -16, -27 -19, -20, -15 -17, -16, -15 -15, -15, -15 -19, -18, -23 -20, -25, -17, -17, -19 -21, -16, -27, -17 -17, -19, -15 -20, -19, -22 -17, -21, -19, -15 -16, -18, -20, -17 -16, -16, -18 -28, -17, -19 -23, -16, -15 -26, -15, -20 -19, -17, -16 -19, -15, -16 -19, -17, -23, -15 -16, -16, -19 -16, -15, -16 -17, -17, -21 -21, -19, -16 -17, -17, -18 -27, -20, -22 -22, -22, -19, -23 -22, -17, -22 -26, -23, -30, -20 -18, -15, -17 -24, -18, -19, -22 -21, -16, -21 transcription factor salivary gland development transcription factor salivary gland morphogenesis transcription factor mesoderm development transcription factor autophagy, induction of apoptosis by hormones transcription factor ectoderm development transcription factor ectoderm development, mesoderm development transcription factor, RNA binding ectoderm development transcription factor, DNA ectoderm development, visual perception bending transcription factor ectoderm development, cell proliferation multiple (including transcription unknown factor) transcription factor transmission of nerve impulse, sex determination transcription factor axon guidance, axon target recognition, larval walking behavior transcription factor neuronal lineage restriction unknown unknown multiple nicotinic acetylcholine-activated cation-selective channel activity, acetylcholine receptor activity unknown actin binding Rho guanyl-nucleotide exchange factor activity, diacylglycerol binding tRNA specific adenosine deaminase activity RNA binding RNA binding RNA binding RNA binding, DNA binding peptide-aspartate beta- dioxygenase activity unknown unknown binding, structural molecule activity ligase activity protein disulfide isomerase activity neurotransmitter secretion, synaptic vesicle exocytois synaptic vesicle exocytosis, regulation of calcium ion dependent exocytosis multiple (including neural processes) multiple (including neural processes) induction of apoptosis, programmed cell death apoptosis, ovarian ring canal formation multiple purine base metabolism mRNA processing, protein metabolism unknown epidermal growth factor receptor signaling pathway mesoderm development transmembrane receptor protein tyrosine kinase signaling pathway unknown mitotic anaphase cell-cell adhesion, cell-matrix adhesion, signal transduction cellular defense response protein folding, protein modification Page 5 of 11 (page number not for citation purposes)