Marra et al. BMC Cancer 2010, 10:129 http://www.biomedcentral.com/1471-2407/10/129 Page 6 of 9 Figure 3 Identification of gene classes in SW780-shPSCA tumors and cells. To induce PSCA silencing mice or cell in culture were treated with dox (dox +) or leaved untreated (dox -). A) Unsuprvised hierarchical clustering of tumors and cells showed that all samples clustered according to dox treatment. Left dendogram shows results of six dox treated and eight untreated tumors collected at day 25 of tumor growth. Right dendogram shows two independent experiments conducted with cells treated for one week in vitro with dox and compared to untreated cells. B) Gene set enrichment of deregulated gene as a function of PSCA expression. Results of gene set enrichment analysis carried out in SW780-shPSCA tumors (in-vivo) or in SW780-shPSCA cells in culture (in-vitro) according to PSCA expression.