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Gamblin TC, Finkelstein SD, Upsal N, Kaye JD, Blumberg D: Microdissection- based allelotyping: a novel technique to determine the temporal sequence and biological aggressiveness of colorectal cancer. Am Surg 2006, 72(5):445-453. Pre-publication history The pre-publication history for this paper can be accessed here: [http://www.biomedcentral.com/1471-2407/10/101/prepub] doi:10.1186/1471-2407-10-101 Cite this article as: Fumagalli et al.: A rapid, sensitive, reproducible and cost-effective method for mutation profiling of colon cancer and metastatic lymph nodes. BMC Cancer 2010 10:101. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit