Thiebaut et al. BMC Genomics 2012, 13:290 http://www.biomedcentral.com/1471-2164/13/290 Page 8 of 14 Table 4 Predicted sugarcane and sorghum mRNA targets for the high confidence novel miRNAs miRNA ID Sugarcane targetsa sof-miR-Seq7 TC130098,TC151912,TC148492,TC113248,TC152641,TC150124,TC132416,TC114872,TC140938,TC115982,TC120001 sof-miR-Seq15 No TC found sof-miR-Seq42 TC153375,TC149921,TC131074,TC139079 sof-miR-Seq143 No TC found sof-miR-Seq151 TC153350,TC116972,TC137677 sof-miR-Seq155 TC152159,TC125281,TC149817,TC136354 sof-miR-Seq171 TC149921,TC132416,TC152095,TC148248 sof-miR-Seq183 TC149944,TC132416 sof-miR-Seq253 No target found sof-miR-Seq313 TC154059,TC139862,TC140501 sof-miR-Seq345 No TC found sof-miR-Seq375 TC136870,TC153496,TC134975 sof-miR-Seq376 No TC found sof-miR-Seq393 TC152280,TC116268 sof-miR-Seq394 No TC found sof-miR-Seq409 TC121220,TC126485,TC132938,TC125507,TC114017,TC141274,TC147793,TC153834,TC115398,TC154486,TC141931, TC137189,TC132602,TC143298,TC121611,TC150673,TC114871,TC154912,TC129500,TC154465,TC152078,TC154496, TC150491,TC154742,TC131475,TC116420,TC142998,TC146859,TC134345,TC133965,TC153566,TC149558,TC149819, TC154535,TC150277 sof-miR-Seq468 TC153570,TC141767 + sof-miR-Seq488 TC152925 - sof-miR-Seq501 TC151370 - sof-miR-Seq504 TC126404 + sof-miR-Seq509 No target found + sof-miR-Seq511 TC124067,TC118309 + sof-miR-Seq513 TC141387 - sof-miR-Seq536 TC139920,TC130151,TC120175 + sof-miR-Seq538 No TC found - sof-miR-Seq565 No TC found - sof-miR-Seq568 No target found + sof-miR-Seq587 TC139519,TC152280 + sof-miR-Seq594 TC115982,TC149921,TC113248,TC140938,TC132416,TC148248,TC140476 + sof-miR-Seq598 TC124210 + sof-miR-Seq610 TC122224,TC140470,TC145127,TC142562,TC120001,TC150124 + sof-miR-Seq648 TC137080,TC134144,TC117196 + sof-miR-Seq656 No TC found + sof-miR-Seq670 TC138461,TC153815,TC149322,TC146441,TC153901,TC142328,TC154437,TC154455,TC125410 + sof-miR-Seq678 TC133894,TC129132 + sof-miR-Seq700 TC152242,TC146024,TC117281,TC152372,TC146587,TC153355,TC154348,TC148421,TC128182,TC148979,TC120001, + TC117481,TC134335,TC113000,TC138157,TC130788,TC129900 sof-miR-Seq720 TC125467,TC129883,TC145851,TC144060 + a Identification of sugarcane targets. miRNAs that only have singletons as targets were identified by No TC found. MiRNAs that did not have predicted targets were identified by No target found. b Plus and minus signals indicate presence or absence (respectively) of sorghum genes containing conserved miRNA recognition sites. Sorghum targetsb + + + + + + + + + + - + - + - +