Wang et al. BMC Genomics 2012, 13:529 http://www.biomedcentral.com/1471-2164/13/529 8. Liu SS, De Barro PJ, Xu J, Luan JB, Zang LS, Ruan YM, Wan FH: Asymmetric mating interactions drive widespread invasion and displacement in a whitefly. Science 2007, 318:1769–1772. 9. Jiu M, Zhou XP, Tong L, Xu J, Yang X, Wan FH, Liu SS: Vector-virus mutualism accelerates population increase of an invasive whitefly. PLoS One 2007, 2:e182. 10. Crowder DW, Horowitz AR, De Barro PJ: Mating behaviour, life history and adaptation to insecticides determine species exclusion between whiteflies. J Anim Ecol 2010, 79:563–570. 11. Gorman K, Slater R, Blande JD, Clarke A, Wren J, McCaffery A, Denholm I: Cross-resistance relationships between neonicotinoids and pymetrozine in Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag Sci 2010, 66:1186–1190. 12. Czosnek H, Ghanim M, Ghanim M: The circulative pathway of begomoviruses in the whitefly vector Bemisia tabaci; insights from studies with Tomato yellow leaf curl virus. Ann Appl Biol 2002, 140:215–231. 13. Dalton R: Whitefly infestations: the Christmas Invasion. Nature 2006, 443:898–900. 14. Naranjo SE, Castle SJ, De Barro PJ, Liu SS: Population Dynamics, Demography, Dispersal and Spread of Bemisia tabaci. In Bemisia: Bionomics and Management of a Global Pest. Edited by Stansly PA, Naranjo SE. Heidelberg: Springer; 2010:185–226. 15. Brown JK, Frohlich DR, Rosell RC: The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annu Rev Entomol 1995, 40:511–534. 16. Boykin LM, Shatters RG, Rosell RC, McKenzie CL, Bagnall RA, De Barro PJ, Frohlich DR: Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Mol Phylogenet Evol 2007, 44:1306–1319. 17. Luan JB, Xu J, Lin KK, Zalucki MP, Shu-sheng L: Species exclusion between an invasive and an indigenous whitefly on host plants with differential levels of suitability. J Integr Agri 2012, 11:215–224. 18. Muñiz M, Nombela G: Differential variation in development of the B- and Q-biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on sweet pepper at constant temperatures. Environ Entomol 2001, 30:720–727. 19. Nauen R, Stumpf N, Elbert A: Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag Sci 2002, 58:868–875. 20. Horowitz AR, Kontsedalov S, Khasdan V, Ishaaya I: Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resistance. Arch Insect Biochem Physiol 2005, 58:216–225. 21. Fernandez E, Gravalos C, Haro PJ, Cifuentes D, Bielza P: Insecticide resistance status of Bemisia tabaci Q-biotype in south-eastern Spain. Pest Manag Sci 2009, 65:885–891. 22. Ghanim M, Kontsedalov S: Gene expression in pyriproxyfen-resistant Bemisia tabaci Q biotype. Pest Manag Sci 2007, 63:776–783. 23. Stewart CNJ: Weedy and Invasive Plant Genomics. New Jersey: Wiley- Blackwell; 2009. 24. Charlesworth D, Charlesworth B, McVean GA: Genome sequences and evolutionary biology, a two-way interaction. Trends Ecol Evol 2001, 16:235–242. 25. Wang P, Sun DB, Qiu BL, Liu SS: The presence of six putative species of the whitefly Bemisia tabaci complex in China as revealed by crossing experiments. Insect Sci 2011, 18:67–77. 26. Sun DB, Xu J, Luan JB, Liu SS: Reproductive incompatibility between the B and Q biotypes of the whitefly Bemisia tabaci: genetic and behavioural evidence. Bull Entomol Res 2011, 101:211–220. 27. Moya A, Guirao P, Cifuentes D, Beitia F, Cenis JL: Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Mol Ecol 2001, 10:891–897. 28. Elbaz M, Weiser M, Morin S: Asymmetry in thermal tolerance trade-offs between the B and Q sibling species of Bemisia tabaci (Hemiptera: Aleyrodidae). J Evol Biol 2011, 24:1099–1109. 29. Wang XW, Luan JB, Li JM, Bao YY, Zhang CX, Liu SS: De novo characterization of a whitefly transcriptome and analysis of its gene expression during development. BMC Genomics 2010, 11:400. 30. Wang XW, Luan JB, Li JM, Su YL, Xia J, Liu SS: Transcriptome analysis and comparison reveal divergence between two invasive whitefly cryptic species. BMC Genomics 2011, 12:458. 31. Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, Li S, Yang H, Wang J, Wang J: De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010, 20:265–272. Page 12 of 13 32. Elmer KR, Fan S, Gunter HM, Jones JC, Boekhoff S, Kuraku S, Meyer A: Rapid evolution and selection inferred from the transcriptomes of sympatric crater lake cichlid fishes. Mol Ecol 2010, 19(Suppl 1):197–211. 33. Yin Y, Martin J, Abubucker S, Scott AL, McCarter JP, Wilson RK, Jasmer DP, Mitreva M: Intestinal transcriptomes of nematodes: comparison of the parasites Ascaris suum and Haemonchus contortus with the free-living Caenorhabditis elegans. PLoS Negl Trop Dis 2008, 2:e269. 34. Zang LS, Chen WQ, Liu SS: Comparison of performance on different host plants between the B biotype and a non-B biotype of Bemisia tabaci from Zhejiang, China. Entomol Exp Appl 2006, 121:221–227. 35. Yang Z, Yoder AD: Estimation of the transition/transversion rate bias and species sampling. J Mol Evol 1999, 48:274–283. 36. Shen JC, Rideout WM 3rd, Jones PA: The rate of hydrolytic deamination of 5- methylcytosine in double-stranded DNA. Nucleic Acids Res 1994, 22:972–976. 37. Xu J, De Barro PJ, Liu SS: Reproductive incompatibility among genetic groups of Bemisia tabaci supports the proposition that the whitefly is a cryptic species complex. Bull Entomol Res 2010, 100:359–366. 38. Makalowski W, Boguski MS: Evolutionary parameters of the transcribed mammalian genome: an analysis of 2,820 orthologous rodent and human sequences. Proc Nat Acad Sci USA 1998, 95:9407–9412. 39. Hellmann I, Zollner S, Enard W, Ebersberger I, Nickel B, Paabo S: Selection on human genes as revealed by comparisons to chimpanzee cDNA. Genome Res 2003, 13:831–837. 40. Nielsen R, Yang Z: Likelihood models for detecting positively selected amino acid sites and applications to the HIV-1 envelope gene. Genetics 1998, 148:929–936. 41. Wondji CS, Hemingway J, Ranson H: Identification and analysis of single nucleotide polymorphisms (SNPs) in the mosquito Anopheles funestus, malaria vector. BMC Genomics 2007, 8:5. 42. Morlais I, Poncon N, Simard F, Cohuet A, Fontenille D: Intraspecific nucleotide variation in Anopheles gambiae: new insights into the biology of malaria vectors. Am J Trop Med Hyg 2004, 71:795–802. 43. Kim SY, Lohmueller KE, Albrechtsen A, Li Y, Korneliussen T, Tian G, Grarup N, Jiang T, Andersen G, Witte D, Jorgensen T, Hansen T, Pedersen O, Wang J, Nielsen R: Estimation of allele frequency and association mapping using next-generation sequencing data. BMC Bioinformatics 2011, 12:231. 44. Luo C, Jones CM, Devine G, Zhang F, Denholm I, Gorman K: Insecticide resistance in Bemisia tabaci biotype Q (Hemiptera: Aleyrodidae) from China. Crop Prot 2010, 29:429–434. 45. Li FF, Xia J, Li JM, Liu SS, Wang XW: p38 MAPK is a component of the signal transduction pathway triggering cold stress response in the MED cryptic species of Bemisia tabaci. J Integr Agri 2012, 11:302–311. 46. Li JM, Ruan YM, Li FF, Liu SS, Wang XW: Gene expression profiling of the whitefly (Bemisia tabaci ) Middle East – Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco. Insect Sci 2011, 18:11–22. 47. Pertea G, Huang X, Liang F, Antonescu V, Sultana R, Karamycheva S, Lee Y, White J, Cheung F, Parvizi B, Tsai J, Quackenbush J: TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics 2003, 19:651–652. 48. Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M: Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 2005, 21:3674–3676. 49. Ye J, Fang L, Zheng H, Zhang Y, Chen J, Zhang Z, Wang J, Li S, Li R, Bolund L, Wang J: WEGO: a web tool for plotting GO annotations. Nucleic Acids Res 2006, 34:W293–W297. 50. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B: Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 2008, 5:621–628. 51. Wolf JB, Bayer T, Haubold B, Schilhabel M, Rosenstiel P, Tautz D: Nucleotide divergence vs. gene expression differentiation: comparative transcriptome sequencing in natural isolates from the carrion crow and its hybrid zone with the hooded crow. Mol Ecol 2010, 19(Suppl 1):162–175. 52. Yang Z, Nielsen R: Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models. Mol Biol Evol 2000, 17:32–43. 53. Zhang Z, Li J, Zhao XQ, Wang J, Wong GK, Yu J: KaKs_Calculator: calculating Ka and Ks through model selection and model averaging. Genomics Proteomics Bioinformatics 2006, 4:259–263. 54. Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004, 32:1792–1797.