Arias et al. BMC Genomics 2011, 12:390 http://www.biomedcentral.com/1471-2164/12/390 Page 12 of 17 Figure 7 Examination of MLP1 expression in mutants lacking Slt2 activation. (a) Expression of MLP1-lacZ was determined in wild-type and the indicated deletant strains growing to mid-log phase in a rich medium. Each value represents the mean and standard deviation of three independent transformants. (b) Slt2 phosphorylation was examined by immunoblotting total extracts from exponential cultures of the wild-type strain and pmt2Δ and ssd1Δ mutants growing in YEPD with an anti-phospho-p44/p42 MAPK antibody. The protein load was monitored using a mouse anti-actin mAb. Figure 8 Transcriptional response observed in mutants pmt2Δ and ssd1Δ is dependent on Slt2 activity. (a) Expression of MLP1- lacZ, CWP1-lacZ and SED1-lacZ was determined in wild-type strain and pmt2Δ, pmt2Δrlm1Δ, pmt2Δslt2Δ, ssd1Δ, ssd1Δrlm1Δ and ssd1Δslt2Δ mutant cells growing in a rich medium under standard conditions. (b) The MLP1-lacZ reporter plasmid was co-transformed with centromeric plasmids bearing the wild-type SLT2 allele, inactive mutant alleles of SLT2 (slt2/mpk1-K54R and slt2/mpk1-TA/YF) or the empty vector (pRS315) into pmt2Δslt2Δ and ssd1Δslt2Δ strains, and b-galactosidase activity was measured in crude extracts. Each value represents the mean and standard deviation of three independent transformants.