Gassmann et al. BMC Cancer 2010, 10:177 http://www.biomedcentral.com/1471-2407/10/177 43. Korb T, Schlüter K, Enns A, Spiegel HU, Senninger N, Nicolson GL, Haier J: Integrity of actin fibers and microtubules influences metastatic tumor cell adhesion. Exp Cell Res 2004, 299:236-247. 44. Varki A: Selectin ligands. Proc Natl Acad Sci USA 1994, 91:7390-7397. Review. 45. Khaldoyanidi SK, Glinsky VV, Sikora L, Glinskii AB, Mossine VV, Quinn TP, Glinsky GV, Sriramarao P: MDA-MB-435 Human breast carcinoma cell homo- and heterotypic adhesion under flow conditions is mediated in part by Thomson-Friedenreich antigen - galectin-3 interactions. J Biol Chem 2003, 278:4127-4134. 46. Heimburg J, Yan J, Morey S, Glinskii OV, Huxley VH, Wild L, Klick R, Roy R, Glinsky VV, Rittenhouse-Olson K: Inhibition of spontaneous breast cancer metastasis by anti-Thomsen-Friedenreich antigen monoclonal antibody JAA-F11. Neoplasia 2006, 8:939-948. 47. Humphries MJ, Olden K, Yamada KM: A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science 1986, 233:467-470. 48. Vollmers HP, Imhof BA, Braun S, Waller CA, Schirrmacher V, Birchmeier W: Monoclonal antibodies which prevent experimental lung metastases. Interference with the adhesion of tumour cells to laminin. FEBS Lett 1984, 172:17-20. 49. Wang H, Fu W, Im JH, Zhou Z, Santoro SA, Iyer V, DiPersio CM, Yu QC, Quaranta V, Al-Mehdi A, Muschel RJ: Tumor cell alpha3beta1 integrin and vascular laminin-5 mediate pulmonary arrest and metastasis. J Cell Biol 2004, 164:935-941. 50. Deen WM, Lazzara MJ, Myers BD: Structural determinants of glomerular permeability. Am J Physiol Renal Physiol 2001, 281:F579-F596. 51. Couchman JR, Beavan LA, McCarthy KJ: Glomerular matrix: synthesis, turnover and role in mesangial expansion. Kidney Int 1994, 45:328-335. 52. Tani N, Higashiyama S, Kawaguchi N, Madarame J, Ota I, Ito Y, Ohoka Y, Shiosaka S, Takada Y, Matsuura N: Expression level of integrin alpha 5 on tumour cells affects the rate of metastasis to the kidney. Br J Cancer 2003, 88:327-333. 53. Kikkawa H, Kaihou M, Horaguchi N, Uchida T, Imafuku H, Takiguchi A, Yamazaki Y, Koike C, Kuruto R, Kakiuchi T, Tsukada H, Takada Y, Matsuura N, Oku N: Role of integrin alpha(v)beta3 in the early phase of liver metastasis: PET and IVM analyses. Clin Exp Metastasis 2002, 19:717-725. 54. Braet F, Wisse E: Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review. Comp Hepatol 2002, 1:1. 55. Rosenow F, Ossig R, Thormeyer D, Gasmann P, Schlüter K, Brunner G, Haier J, Eble JA: Integrins as antimetastatic targets of RGD-independent snake venom components in liver metastasis [corrected]. Neoplasia 2008, 10:168-176. Erratum in: Neoplasia 2008: 10: 410. 56. Orr FW, Wang HH, Lafrenie RM Scherbarth S, Nance DM: Interactions between cancer cells and the endothelium in metastasis. J Pathol 2000, 190:310-329. 57. Takenaka K, Shibuya M, Takeda Y, Hibino S, Gemma A, Ono Y, Kudoh S: Altered expression and function of beta1 integrins in a highly metastatic human lung adenocarcinoma cell. Int J Oncol 2000, 17:1187-1194. 58. Orr FW, Adamson IY, Young L: Promotion of pulmonary metastasis in mice by bleomycin-induced endothelial injury. Cancer Res 1986, 46:891-897. Pre-publication history The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2407/10/177/prepub doi: 10.1186/1471-2407-10-177 Cite this article as: Gassmann et al., In vivo tumor cell adhesion in the pul- monary microvasculature is exclusively mediated by tumor cell - endothelial cell interaction BMC Cancer 2010, 10:177 Page 11 of 11