Urzúa et al. BMC Genomics 2010, 11(Suppl 5):S1 http://www.biomedcentral.com/1471-2164/11/S5/S1 Page 5 of 11 Functional analysis of genes associated both to OTF and NL Table 2 also shows the number of genes correlated with more than one trait. The highest overlap was 145 clones associated both with OTF and NL. No overlap was observed between OTF and litter size (LS), or between OTF and relative fecundity (RF). The latter parameter -numerically derived from NL, LS and PM [5] - resulted in 17 correlated genes, 14 of them also correlated to NL and LS, 7 genes each trait. Interestingly, LS showed the highest percentage (90%) of exclusively correlated genes. Figure 3 shows scatter plots for a combined total of 40 Figure 3 Ovarian gene expression correlated to OTF and NL The gene expression ratios of the top-20 OTF(-), NL(+) best correlated clones (A) and the top-10 OTF(+), NL(-) best correlated clones (B) are plotted against their respective phenotypic variables. The average of squared correlation coefficients and four representative tendency lines are shown in each plot. Insets show hierarchical clusters for genes (vertical trees) and samples (horizontal trees). Yellow clusters represent negative correlation and blue clusters represent positive correlation. Color scales and Unigene IDs are shown at the bottom and right side of each cluster, respectively. Unknown clones appear with the NIA-15K clone code.