BMC Genomics 2007, 8:271 Additional material Additional file 1 Supplement to Figure 3. The precise positions in human NOS-2 promoter of all the TFBSs shown in Fig. 3 Click here for file [http://www.biomedcentral.com/content/supplementary/1471- 2164-8-271-S1.doc] Additional file 2 Proximal promoters of dog, mouse, rat and human. The alignment of the proximal promoters of dog, mouse, rat and human, showing the presence of a simple (A)n repeat next to the κB site in the human sequence. Click here for file [http://www.biomedcentral.com/content/supplementary/1471- 2164-8-271-S2.doc] Additional file 3 EnsEMBL Gene IDs. All EnsEMBL Gene IDs and chromosomal locations of the sequences used in the study. Click here for file [http://www.biomedcentral.com/content/supplementary/1471- 2164-8-271-S3.doc] Acknowledgements The authors thank Dr S. Bartlett for help in the preparation of the manu- script. The authors also thank Dr J. Dopazo for fruitful discussion and com- ments in the development of the project. This work was supported by a grant from Ministerio de Educación y Ciencia (SAF2005-03022), BIO 283- 2006 (Comunidad de Madrid), RECAVA and Fundación Mutua Madrileña. DR and JMV were supported by fellowships from MEC (Spain). References 1. Kracht M, Saklatvala J: Transcriptional and post-transcriptional control of gene expression in inflammation. Cytokine 2002, 20:91-106. 2. Natoli G: Tuning up inflammation: how DNA sequence and chromatin organization control the induction of inflamma- tory genes by NF-κB. FEBS Lett 2006, 580:2843-2849. 3. Natoli G: Little things that count in transcriptional regulation. Cell 2004, 118:406-408. 4. Ito CY, Kazantsev AG, Baldwin AS Jr.: Three NF-k B sites in the IκB-α promoter are required for induction of gene expres- sion by TNF-α. Nucleic Acids Res 1994, 22:3787-3792. 5. Bates PW, Miyamoto S: Expanded nuclear roles for IκBs. Sci STKE 2004, 2004:e48. 6. 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