Herzig et al. BMC Genomics 2010, 11:100 http://www.biomedcentral.com/1471-2164/11/100 protocol. cDNA clones were sequenced commercially (GeneWiz, South Plainfield, NJ) in order to verify the insert identity and for subsequent sequence analysis. Additional file 1: Germline TRDD and TRDJ gene sequences Click here for file [ http://www.biomedcentral.com/content/supplementary/1471-2164-11- 100-S1.PDF ] Acknowledgements This work was conducted as part of the Bovine Genome Annotation Sequencing Consortium. The work was supported by USDA-NRI grants nos. 2006-01691 and 2005-01812 and CSREES-USDA Massachusetts Agricultural Experiment Station under project no. MAS00913 and Multi-State NRSP-008 project no. MAS00955. Author details 1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA. 2IMGT, Institut Universitaire de France, Laboratoire d’Immuno Génétique Moléculaire, Université Montpellier 2, UPR CNRS 1142, Montpellier, France. 3Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA. 4Current address: Department of Epidemiology, Columbia University, New York, NY 10032, USA. Authors’ contributions CH carried out the annotations, molecular studies and the sequence analyses and helped to draft the manuscript. MPL participated in interpretation of data and helped to draft the manuscript. CB participated in the design of the study, interpretation of data, securing funding and helped to draft the manuscript. All authors read and approved the final manuscript. Received: 2 June 2009 Accepted: 9 February 2010 Published: 9 February 2010 References 1. Chien YH, Jores R, Crowley MP: Recognition by gamma/delta T cells. 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