BMC Cancer 2008, 8:57 http://www.biomedcentral.com/1471-2407/8/57 Table 1: Correlation of FOXP3+ Tregs, B7-H1+ TIL and PD-1+ TIL with the clinicopathological parameters of 62 breast cancer patients Age < 40 years ≥ 40 years Tumor Size <4 CM ≥ 4 CM Lymph Node Metastasis Negative 1 to 3 4 to 9 10 or more Histological Grade (SBR) II III Lymphovascular Invasion Positive Negative Her2/neu status Positive Negative ER status Positive Negative PR status Positive Negative Neo-adjuvant Chemotherapy With Without Lymphocyte Infiltration Focal Moderate Severe B7-H1 expression of Tumor cells Positive Negative + 15 (68)♣ 27 (68) 18 (55) 24 (83) 15 (75) 5 (42) 12 (67) 9 (90) 14 (45) 28 (90) 26 (70) 16 (64) 13 (62) 16 (64) 24 (57) 18 (90) 17 (57) 25 (78) 23 (64) 19 (73) 14 (52) 14 (74) 14 (88) 17 (89) 25 (58) FOXP3 - 7 (32) 13 (33) 15 (45) 5 (17) 5 (25) 7 (58) 6 (33) 1 (10) 17 (56) 3 (10) 11 (30) 9 (36) 8 (38) 22 (58) 18 (43) 2 (10) 13 (43) 7 (22) 13 (36) 7 (27) 13 (48) 5 (26) 2 (13) 2 (11) 18 (42) *P 1.000 0.029 0.107 <0.001 0.782 0.588 0.010 0.103 0.584 0.051 0.019 + 12 (55) 25 (63) 16 (48) 21 (72) 13 (65) 6 (50) 11 (61) 6 (60) 12 (39) 25 (81) 25 (68) 12 (48) 18 (62) 19 (58) 21 (50) 16 (80) 15 (50) 22 (69) 20 (56) 17 (65) 12 (44) 11 (58) 14 (88) 17 (89) 20 (47) B7-H1 - 10 (45) 15 (34) 17 (52) 8 (28) 7 (35) 6 (50) 7 (39) 4 (40) 19 (61) 6 (19) 12 (32) 13 (52) 11 (38) 14 (42) 21 (50) 4 (20) 15 (50) 10 (31) 16 (44) 9 (35) 15 (56) 8 (42) 2 (13) 2 (11) 23 (53) P 0.595 0.072 0.864 0.002 0.187 0.798 0.029 0.195 0.600 0.017 0.002 + 15 (68) 22 (58) 17 (53) 20 (71) 12 (63) 2 (29) 12 (67) 5 (56) 12 (40) 25 (83) 23 (64) 14 (58) 17 (61) 20 (63) 19 (48) 18 (90) 11 (38) 26 (84) 23 (66) 14 (56) 12 (46) 13 (68) 12 (80) 15 (83) 22 (52) PD-1 - 7 (32) 16 (42) 15 (47) 8 (29) 7 (37) 5 (71) 6 (33) 4 (44) 18 (60) 5 (17) 13 (36) 10 (42) 11 (39) 12 (38) 21 (53) 2 (10) 18 (62) 5 (16) 12 (34) 11 (44) 14 (54) 6 (32) 3 (20) 3 (17) 20 (48) P 0.583 0.187 0.939 0.001 0.787 1.000 0.001 <0.001 0.591 0.076 0.041 Abbreviations: (+ and -) are number of positive and negative patients, ♣Numbers between brackets are the percentages of patients, Two samples had unknown LN+ status, Two samples had unknown PD-1 status *P values in bold represent a significant data. mainly expressed by two different T lymphocyte subsets (Figure 4C) and only very few CD8+ TIL (< 5%) were FOXP3+. Therefore, it seems that most FOXP3+ Tregs described here are of the CD4+ subset since they were restricted to CD3+ T lymphocytes. On the other hand PD- 1 molecule was mainly expressed by CD8+ T lymphocytes with only few CD8- population were positive for PD-1 (Figure 4D). B7-H1 was mainly expressed by CD8- T lym- phocytes (Figure 4E) consistent with our previous obser- vation of its expression by CD4+ T lymphocytes [18]. Page 6 of 12 (page number not for citation purposes)