50000 40000 30000 20000 10000 0 50000 40000 30000 20000 ts 10000 in 0 u y r a r t i b r a / y it 50000 sn 40000 te 30000 In 20000 10000 0 50000 40000 30000 20000 10000 0 A ARF B C D Ras-like small GTPases ARL Rab Ras-like BARP ESAG6/7 ISG65 ISG75 ISG65-like Qa Qb Qc R Surface proteins SNAREs Secretory pepidases BMC Genomics 2008, 9:298 http://www.biomedcentral.com/1471-2164/9/298 Aspartic/cysteine Serine Metallo Open reading frame accession BSF PCF RFeiglautrivee3steady state mRNA levels of selected trypanosome genes Relative steady state mRNA levels of selected trypanosome genes. Fluorescence intensity data for eight microarray experiments comparing BSF to PCF, representing four biological replicates plus dye-swaps, was normalised to the total signal intensity of each slide, averaged and plotted as bar-graphs against ORF designation or accession number. Error bars indicate the standard deviation, dark bars are BSF and light bars PCF. Panel A: Small GTPases. ARL and Rab annotations are based on [39], Ras-like annotations based on [29] and additional data at GeneDB. Panel B: Cell surface proteins. Panel C: SNARE family proteins. Annotations based on domain architecture and similarity to sequences described for L. major [59]. Panel D: Putative secretory pathway proteases. ORFs were included for predicted proteases that bear an ER targeting sequence and/or a pre- dicted trans-membrane domain. In each cohort, a limited number of transcripts are highly expressed. Page 9 of 28 (page number not for citation purposes)