Natarajan et al. BMC Genomics 2010, 11:606 http://www.biomedcentral.com/1471-2164/11/606 20. Umezawa T, Sakurai T, Totoki Y, Toyoda A, Seki M, Ishiwata A, Akiyama K, Kurotani A, Yoshida T, Mochida K, Kasuga M, Todaka D, Maruyama K, Nakashima K, Enju A, Mizukado S, Ahmed S, Yoshiwara K, Harada K, Tsubokura Y, Hayashi M, Sato S, Anai T, Ishimoto M, Funatsuki H, Teraishi M, Osaki M, Shinano T, Akashi R, Sakaki Y, Shinozaki KY, Shinozaki K: Sequencing and Analysis of Approximately 40 000 Soybean cDNA Clones from a Full-Length-Enriched cDNA Library. DNA Research 2008, 15:333-346. 21. Naoumkina M, Jerez IT, Allen S, He J, Zhao PX, Dixon RA, May GD: Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub). BMC Plant Biology 2007, 7:62. 22. Clarke BC, Hobbs M, Skylas D, Appels R: Genes active in developing wheat endosperm. Funct Integr Genomics 2000, 1:44-55. 23. Rao KS, Chakrabarti PP, Rao VSK, Prasad RBN: Phospholipid Composition of Jatropha curcas Seed Lipids. J Am Oil Chem Soc 2009, 86:197-200. 24. Holmstrom KO, Somersalo S, Mandal A, Palva TE, Welin B: Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. Journal of Experimental Botany 2000, 51:177-185. 25. Liang C, Zhang XY, Luo Y, Wang GP, Zou Q, Wang W: Overaccumulation of Glycine Betaine Alleviates the Negative Effects of Salt Stress in Wheat. Russian Journal of Plant Physiology 2009, 56:370-376. 26. Sarowar S, Oh HW, Cho HS, Baek KH, Seong ES, Joung YH, Cho GJ, Lee S, Choi D: Capsicum annuum CCR4-associated factor CaCAF1 is necessary for plant development and defence response. The Plant Journal 2007, 51:792-802. 27. Ding X, Cao Y, Huang L, Zhao J, Xu C, Li X, Wang S: Activation of the Indole-3-Acetic Acid-Amido Synthetase GH3-8 Suppresses Expansin Expression and Promotes Salicylate- and Jasmonate-Independent Basal Immunity in Rice. The Plant Cell 2008, 20:228-240. 28. Lorenzo O, Piqueras R, Sánchez-Serrano JJ, Solano R: ETHYLENE RESPONSE FACTOR 1 Integrates Signals from Ethylene and Jasmonate Pathways in Plant Defense. The Plant Cell 2002, 15:165-178. 29. Gu Z, Ma B, Jiang Y, Chen Z, Su X, Zhang H: Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses. Gene 2008, 415:1-12. 30. Ondzighi CA, Christopher DA, Cho EJ, Chang SC, Staehelin LA: Arabidopsis Protein Disulfide Isomerase-5 Inhibits Cysteine Proteases during Trafficking to Vacuoles before Programmed Cell Death of the Endothelium in Developing Seeds. The Plant Cell 2008, 20:2205-2220. 31. Damer CK, Bayeva M, Hahn ES, Rivera J, Socec CI: Copine A, a calcium- dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium. BMC Cell Biology 2005, 6:46. 32. Turner NC, Furbank RT, Berger JD, Gremigni P, Abbo S, Leport L: Seed Size Is Associated with Sucrose Synthase Activity in Developing Cotyledons of Chickpea Crop. Science 2009, 49:621-627. 33. Bentham G, Hooker JD: Genera Plantarum. L. Reeve & Co, London 1862. 34. APG II: An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Botanical Journal of the Linnean Society 2003, 141:399-436. 35. APG III: An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Botanical Journal of the Linnean Society 2009, 161:105-121. 36. Angiosperm Phylogeny Website. [http://www.mobot.org/mobot/research/ apweb]. 37. Sharma N, Anderson M, Kumar A, Zhang Y, Giblin EM, Abrams SR, Zaharia LI, Taylor DC, Fobert PR: Transgenic increases in seed oil content are associated with the differential expression of novel Brassica -specific transcripts. BMC Genomics 2008, 9:619. 38. Maisonneuve S, Bessoule JJ, Lessire R, Delseny M, Roscoe TJ: Expression of Rapeseed microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis. Plant Physiology 2010, 152:670-684. 39. Jain RK, Coffey M, Lai K, Kumar A, MacKenzie SL: Enhancement of seed oil content by expression of glycerol-3-phosphate acyltransferase genes. Biochemical Society Transactions 2000, 28:958-961. 40. Aghoram K, Wilson RF, Burton JW, Dewey RE: A mutation in a 3-Keto-Acyl- ACP Synthase II Gene is associated with elevated Palmitic acid levels in Soybean seeds. Crop Science 2006, 46:2453-2459. Page 13 of 14 41. Knutzon DS, Gregory A, Thompson , Sharon E, Radke , William B, Johnson , Knauf VC, kridlt JC: Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc Natl Acad Sci 1992, 89:2624-2628. 42. Goepfert S, Poirier Y: β-Oxidation in fatty acid degradation and beyond. Current Opinion in Plant Biology 2007, 10:245-251. 43. Gao S, Ouyang C, Wang S, Xu Y, Tang L, Chen F: Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environ 2008, 54:374-381. 44. Xiong L, Zhu JK: Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell and Environment 2002, 25:131-139. 45. Kumar S, Dhingra A, Daniell H: Plastid- Expressed Betaine Aldehyde Dehydrogenase Gene in Carrot Cultured Cells, Roots, and Leaves Confers Enhanced Salt Tolerance. Plant Physiology 2004, 136:2843-2854. 46. Wu W, Su Q, Xia XY, Wang Y, Luan YS, An LJ: The Suaeda liaotungensis kitag betaine aldehyde dehydrogenase gene improves salt tolerance of transgenic maize mediated with minimum linear length of DNA fragment. Euphytica 2008, 159:17-25. 47. Jia GX, Zhu ZQ, Chang FQ, Li YX: Transformation of tomato with the BADH gene from Atriplex improves salt tolerance. Plant Cell Rep 2002, 21:141-146. 48. Zhang FL, Niu B, Wang YC, Chen F, Wang SH, Xu Y, Jiang LD, Gao S, Wu J, Tang L, Jia YJ: A novel betaine aldehyde dehydrogenase gene from Jatropha curcas , encoding an enzyme implicated in adaptation to environmental stress. Plant Science 2008, 174:510-518. 49. Nuccio ML, Russell BL, Nolte KD, Rathinasabapathi B, Gage DA, Hanson AD: The endogenous choline supply limits glycine betaine synthesis in transgenic tobacco expressing choline monooxygenase. Plant Journal 1998, 16:487-496. 50. Huang J, Hirji R, Adam L, Rozwadowski KL, Hammerlindl JK, Keller WA, Selvaraj G: Genetic Engineering of Glycinebetaine Production toward Enhancing Stress Tolerance in Plants: Metabolic Limitations. Plant Physiology 2000, 122:747-756. 51. Rajagopal D, Agarwal P, Tyagi W, Singla-Pareek SL, Reddy MK, Sopory SK: Pennisetum glaucum Na+/H+ antiporter confers high level of salinity tolerance in transgenic Brassica juncea. Mol Breeding 2007, 19:137-151. 52. Garg AK, Kim JK, Owens TG, Ranwala AP, Choi YD, Kochian LV, Wu RJ: Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci 2002, 99:15898-15903. 53. Terol J, Conesa A, Colmenero JM, Cercos M, Tadeo T, Agustí J, Alós E, Andres F, Soler G, Brumos J, Iglesias DJ, Götz S, Legaz F, Argout X, Courtois B, Ollitrault P, Dossat C, Wincker P, Morillon R, Talon M: Analysis of 13000 unique Citrus clusters associated with fruit quality, production and salinity tolerance. BMC Genomics 2007, 8:31. 54. Ho CL, Kwan YY, Choi MC, Tee SS, Ng WH, Lim KA, Lee YP, Ooi SE, Lee WW, Tee JM, Tan SH, Kulaveerasingam H, Alwee SS, Abdullah MO: Analysis and functional annotation of expressed sequence tags(ESTs) from multiple tissues of oil palm (Elaeis guineensis Jacq). BMC Genomics 2007, 8:381. 55. Yoshida S, Ishida JK, Kamal NM, Ali AM, Namba S, Shirasu K: A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica. BMC Plant Biology 2010, 10:55. 56. Fukuoka H, Yamaguchi H, Nunome T, Negoro S, Miyatake K, Ohyama A: Accumulation, functional annotation, and comparative analysis of expressed sequence tags in eggplant (Solanum melongena L.), the third pole of the genus Solanum species after tomato and potato. Gene 2010, 450:76-84. 57. Nanjo T, Sakurai T, Totoki Y, Toyoda A, Nishiguchi M, Kado T, Igasaki T, Futamura N, Seki M, Sakaki Y, Shinozaki K, Shinohara K: Functional annotation of 19,841 Populus nigra full-length enriched cDNA clones. BMC Genomics 2007, 8:448. 58. O’Hara P, Slabas AR, Fawcett T: Fatty Acid and Lipid Biosynthetic Genes Are Expressed at Constant Molar Ratios But Different Absolute Levels during Embryogenesis. Plant Physiology 2002, 129:310-320. 59. Patanjali SR, Parimoo S, Weissman SM: Construction of a uniform- abundance (normalized) cDNA library. Proc Natl Acad Sci 1991, 88:1943-1947. 60. Soares MB, Bonaldo MF, Jelene P, Su L, Lawton L, Efstratiadis A: Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci 1994, 91:9228-9232. 61. National Centre for Biotechnology Information BLAST. [http://blast.ncbi. nlm.nih.gov/Blast.cgi].