Romeuf et al. BMC Genomics 2010, 11:185 http://www.biomedcentral.com/1471-2164/11/185 Availability and requirements The wheat transcription factor inventory database wDBTF is now available at http://wwwappli.nantes.inra. fr:8180/wDBFT/ License: none. Any restrictions to use by non-academics: none. Additional file 1: The 226 wheat ESTs libraries used for TF analysis. A table showing banks used for this database construction and corresponding tissue and EST number. Additional file 2: Sequences of primers used for detection of Dof transcripts. A table of sequence accession names and DNA probe sequences of forward and reverse primers used for Dof transcripts PCR amplification. Additional file 3: Multiple sequence alignment of the Dof DNA- binding domain from wheat, rice and barley. A protein sequence alignment of the Dof domain resulting from CLUSTALW. The levels of amino acid conservation at each position among the Dof members are indicated in the first histogram with the highest bars representing 100% amino acid identity. The black histogram represents the consensus amino acid sequence. Additional file 4: Tissue-specific in silico expression of wheat Dof singletons. A table showing EST accession names of wheat Dof singletons and their corresponding tissue of origin. Acknowledgements We thank Christophe Troquier and his team for growing the plants and Stéphanie Verrier for RNA extraction. We also thank Rachel Carol from Emendo Bioscience http://www.emendo.co.uk for editing the manuscript. Author details 1Institut National de la Recherche Agronomique (INRA), UMR1095, Génétique, Diversité et Ecophysiologie des Céréales, 234 avenue du Brézet, Clermont-Ferrand, F-63100 France; Université Blaise-Pascal, UMR1095, Campus des Cézeaux, F-63170 Aubière, France. 2Institut National de la Recherche Agronomique (INRA), UR Biopolymères, Interactions, Assemblages (BIA), rue de la Géraudière, Nantes, F-44316, France. Authors’ contributions IR analyzed data, designed and assessed specific primers for Dof transcript amplification and wrote the manuscript. DT has developed bioinformatics tools. MD carried out the RNA extraction and PCR amplification. CR performed the Blast searches against Affymetrix probes, participated in coordination and in the design of the study and helped to draft the manuscript. GB and GC participated in coordination and helped to draft the manuscript. All authors read and approved the final manuscript. 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