1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 AT4G27170 AT4G27160 AT4G27150 AT4G28520 AT3G27660 AT5G38195 AT4G25140 AT5G44120 AT5G45890 AT1G03880 AT1G48750 AT1G79870 AT1G12920 AT5G35530 AT3G12580 AT3G01570 AT1G69830 AT3G05020 AT5G09440 6,691 2,402 249 126 1,226 1,640 1,119 1,112 4,452 1,791 8 5 1 401 31 170 1 401 31 96 67 34 24 13 15 36 15 17 44 12 4 3 2 6 8 6 2 14 3 669 637 575 560 195 183 161 146 143 143 138 136 123 113 102 102 99 97 86 82 BMC Genomics 2009, 10:295 http://www.biomedcentral.com/1471-2164/10/295 Table 5: Summary of the 20 most abundant genes expressed in B. napus seeds at 35 days after pollination (DAP) based on LongSAGE data analysis. Rank A. thaliana locus No. of matched Brassica ESTs at 1e-6 AT4G27140 16,413 No. of different tags matched to Brassica ESTs Mean tag count per locus Frequency of tag counts (%, normalized) 2.657 2.528 2.284 2.224 0.776 0.728 0.640 0.580 0.567 0.566 0.549 0.538 0.487 0.447 0.406 0.405 0.393 0.385 0.340 0.326 Description of matched A. thaliana gene locus 2S seed storage protein 1/ 2S albumin storage protein/NWMU1-2S albumin 1 2S seed storage protein 4/ 2S albumin storage protein/NWMU2-2S albumin 4 2S seed storage protein 3/ 2S albumin storage protein/NWMU2-2S albumin 3 2S seed storage protein 2/ 2S albumin storage protein/NWMU2-2S albumin 2 CRU3 (CRUCIFERIN 3) OLEO4 (OLEOSIN4) protease inhibitor/seed storage/lipid transfer protein (LTP) family protein OLEO1 (OLEOSIN1) CRA1 (CRUCIFERINA); nutrient reservoir SAG12 (SENESCENCE- ASSOCIATED GENE 12); cysteine-type peptidase CRU2 (CRUCIFERIN 2); nutrient reservoir protease inhibitor/seed storage/lipid transfer protein (LTP) family protein oxidoreductase family protein ERF1-2 (EUKARYOTIC RELEASE FACTOR 1-2); translation release factor 40S ribosomal protein S3 (RPS3C) HSP70 (heat shock protein 70); ATP binding glycine-rich protein/ oleosin AMY3/ATAMY3 (ALPHA- AMYLASE-LIKE 3); alpha- amylase ACP1 (ACYL CARRIER PROTEIN 1) phosphate-responsive protein, putative Page 7 of 16 (page number not for citation purposes)