Romeuf et al. BMC Genomics 2010, 11:185 http://www.biomedcentral.com/1471-2164/11/185 Page 8 of 15 Figure 3 Visualization of the wDBTF web interface main page. The list of available families links to an individual page for each of the 84 analyzed TF subfamilies and then to the results page from which wheat sequences and additional information can be downloaded. MCOG-A, groups III and VII to MCOG-C, and groups IV, V and VI to MCOG-D. Expression of T. aestivum Dof genes We found 59 wheat Dof represented by 25 contigs and 34 singletons. Of the Dof contigs 60% are made up of 1- 5 sequences, while only 5 have more than 10 ESTs indi- cative of high in silico expression. Of all the wheat Dof expressed sequences 48% come from spike libraries (Figure 5). In silico expression data show that 25 expressed Dof sequences from six contigs (Figure 6) and 19 singletons (Additional file 4) were detected only in spikes. Our primary focus is on iden- tifying wheat Dof involved in endosperm development. cDNA made from RNA extracted from seed (300 degree-days after anthesis) of an INRA breeding line RE99006 was tested for the presence of 20 Dof tran- scripts using gene-specific PCR primers (Additional file 2); all these Dof transcripts were confirmed to be expressed in seeds (data not shown). To establish whether they are involved in wheat grain development, we tested the expression of 12 transcripts in