Adomas et al. BMC Genomics 2010, 11:477 http://www.biomedcentral.com/1471-2164/11/477 Page 6 of 16 Figure 2 Genes significantly differentially expressed (P ≤ 0.05) by S. cerevisiae growing in nitrogen-poor (light red/blue) and nitrogen- rich (dark red/blue) conditions coding for enzymes involved in glutamate metabolism; identified by microarray using multi-targeted primers (red) or oligo(dT) primers (blue). The results were validated using RNA sequencing on Illumina platform (grey; bars in the same order as for microarray profiling). Error bars represent 95% credible intervals. NS - statistically insignificant difference (P > 0.05); * or lack of a symbol - significant difference in gene expression level (P ≤ 0.05); † - microarray: not well measured; tag sequencing: not detected or too few tags for a statistical significance (P > 0.05).