BMC Genomics 2009, 10:58 http://www.biomedcentral.com/1471-2164/10/58 TFeigmuproer3al expression patterns of probesets for predicted H. glycines secretory protein encoding genes Temporal expression patterns of probesets for predicted H. glycines secretory protein encoding genes. All 646 H. glycines probesets encoding putative secretory proteins with predicted signal peptide, at least thirty amino acids after the sig- nal peptide cleavage site and no predicted transmembrane helix were differentially expressed (FDR 5%) over the entire life cycle and were grouped into nine expression clusters. The average expression pattern of each cluster is represented by a bold line. found 576 G. max and 134 P. sojae probesets that hybrid- ized strongly and repeatedly to H. glycines probes in three independent experiments (Additional files 13, 14) and that showed differential expression (Figures 5, 6). A vari- ety of BLAST searches of cross-hybridizing probesets (BLASTX, BLASTN against non-redundant GenBank; BLASTN against dbEST_other database) detected only a negligible number of Caenorhabditis hits and did not lead to matches for cyst nematode sequences, but primarily soybean and oomycete or fungus sequences as best hits Page 7 of 17 (page number not for citation purposes)