# Patent

## Title
Subtilases

## Abstract

The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 14/013,231 filed on Aug. 29, 2013 (pending, now allowed), which is a continuation of U.S. application Ser. No. 13/743,839 filed on Jan. 17, 2013 (now U.S. Pat. No. 8,546,122), which is a continuation of U.S. application Ser. No. 13/304,880 filed on Nov. 28, 2011 (now U.S. Pat. No. 8,383,387), which is a continuation of U.S. Ser. No. 12/879,410 filed on Sep. 10, 2010 (now U.S. Pat. No. 8,084,241), which is a continuation of U.S. application Ser. No. 11/928,576 filed on Oct. 30, 2007 (now abandoned) which is a continuation of U.S. application Ser. No. 10/786,850 filed Feb. 24, 2004 (now U.S. Pat. No. 7,294,499), which is a continuation of PCT/DK2004/000066 filed Jan. 30, 2004, which claims priority or the benefit under 35 U.S.C. 119 of Danish application nos. PA 2003 00119 and PA 2003 00689 filed Jan. 30, 2003 and May 7, 2003, respectively, and U.S. provisional application Nos. 60/445,300 and 60/468,574 filed Feb. 5, 2003 and May 7, 2003, respectively, the contents of which are fully incorporated herein by reference.


REFERENCE TO SEQUENCE LISTING
The present application contains a paper copy and computer readable form of a sequence listing. The contents of the computer readable form are fully incorporated herein by reference.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to variants of TY145 subtilases and BPN′ subtilases and to methods of construction such variants with altered properties, such as stability (e.g. thermostability or storage stability), Ca2+ dependency, pH dependent activity.
2. Description of Related Art
Enzymes have been used within the detergent industry as part of washing formulations for more than 30 years. Proteases are from a commercial perspective the most relevant enzyme in such formulations, but other enzymes including lipases, amylases, cellulases, hemicellulases or mixtures of enzymes are also often used.
To improve the cost and/or the performance of proteases there is an ongoing search for proteases with altered properties, such as increased activity at low temperatures, increased thermostability, increased specific activity at a given pH, altered Ca2+ dependency, increased stability in the presence of other detergent ingredients (e.g. bleach, surfactants etc.) etc.
The search for proteases with altered properties include both discovery of naturally occurring proteases, i.e. so called wild-type proteases but also alteration of well-known proteases by e.g. genetic manipulation of the nucleic acid sequence encoding said proteases. Knowledge of the relationship between the three-dimensional structure and the function of a protein has improved the ability to evaluate which areas of a protein to alter to affect a specific characteristic of the protein.
One family of proteases, which are often used in detergents, are the subtilases. This family has previously been further grouped into 6 different sub-groups by Siezen R J and Leunissen JAM, 1997, Protein Science, 6, 501-523. One of these sub-groups is the Subtilisin family which includes subtilases such as BPN′, subtilisin 309 (SAVINASE®, Novozymes A/S), subtilisin Carlsberg (ALCALASE®, Novozymes A/S), subtilisin S41 (a subtilase from the psychrophilic Antarctic Bacillus TA41, Davail S et al. 1994, The Journal of Biological Chemistry, 269(26), 99. 17448-17453), subtilisin S39 (a subtilase from the psychrophilic Antarctic Bacillus TA39, Narinx E et al. 1997, Protein Engineering, 10 (11), pp. 1271-1279) and TY145 (a subtilase from Bacillus sp. TY145, NCIMB 40339 described in WO 92/17577).
However, despite the sequence homology between the subtilases belonging to the Subtilisin subgroup of subtilases, modelling of the three-dimensional structure of one subtilase on the basis of the three-dimensional structure of another subtilase may result in an incorrect three-dimensional structure because of structural differences.
The inventors of the present invention have elucidated the three-dimensional structure of the TY145 subtilase and found that there are several differences between this and the three-dimensional structure of BPN′ also belonging to the Subtilisin subgroup of subtilases. This surprising difference in structure makes it advantageous to use the TY145 structure as basis for homology modelling of TY145 like subtilisins, which, in turn, will improve the ability to obtain desired changes in functionality by protein engineering.
Two studies have used protein engineering to alter functionality of TY145 like subtilisins: Miyazaki K et al. 2000, J Mol Biol, 297, pp. 1015-1026 discloses enhancement of the thermostability and activity of the psychrophilic protease subtilisin S41 by methods of directed evolution.
Wintrode T L et al. 2000, Journal of Biological Chemistry, 275 (41), pp. 31635-31640 discloses conversion of a mesophilic subtilisin-like protease from Bacillus sphaericus SSII into its psychrophilic counterpart by methods of directed evolution. Wintrode et al. constructed the three-dimensional structural model of the SSII subtilase on basis of its homology with subtilisins Carlsberg, Savinase, BPN′ and Thermitase. However, according to the present invention the SSII subtilase pertain to the new group of TY145 like subtilases and thus the modelling of SSII based on the 3D structure of the BPN′ like subtilases will likely give an inaccurate result.
The differences between the three-dimensional structures of TY145 and BPN′ are confirmed by the recently published three-dimensional structure of the subtilase “sphericase” from Bacillus sphaericus (PDB NO:1EA7, Protein Data Bank). The overall structure and many details of this subtilase are very homologous to the TY145 subtilase structure.
BRIEF DESCRIPTION OF THE INVENTION
The inventors have modified the amino acid sequence of a subtilase to obtain variants with improved properties, based on the three-dimensional structure of the subtilases TY145 and BPN′. The variants have altered properties, such as increased activity at low temperatures, increased thermostability, increased specific activity at a given pH, altered Ca2+ dependency, increased stability in the presence of other detergent ingredients (e.g. bleach, surfactants etc.) etc.
Accordingly, the object of the present invention is to provide a method for constructing subtilases having altered properties, in particular to provide a method for constructing subtilases having altered properties as described above.
Thus, in its broadest aspect, the present invention relates to a method for constructing a variant of a parent subtilase, wherein the variant has at least one altered property as compared to said parent subtilase, which method comprises:
i) analyzing the three-dimensional structure of the subtilase to identify, on the basis of an evaluation of structural considerations, at least one amino acid residue or at least one structural region of the subtilase, which is of relevance for altering said property;
ii) constructing a variant of the subtilase, which as compared to the parent subtilase, has been modified in the amino acid residue or structural part identified in i) so as to alter said property; and
iii) testing the resulting subtilase variant for said property.
Although it has been described in the following that modification of the parent subtilase in certain regions and/or positions is expected to confer a particular effect to the thus produced subtilase variant, it should be noted that modification of the parent subtilase in any of such regions may also give rise to any other of the above-mentioned effects. For example, any of the regions and/or positions mentioned as being of particular interest with respect to, e.g., improved thermostability, may also give rise to, e.g., higher activity at a lower pH, an altered pH optimum, or increased specific activity, such as increased peptidase activity.
Further aspects of the present invention relates to variants of a subtilase, the DNA encoding such variants and methods of preparing the variants. Still further aspects of the present invention relates to the use of the variants for various industrial purposes, in particular as an additive in detergent compositions. Other aspects of the present invention will be apparent from the below description as well as from the appended claims.
BRIEF DESCRIPTION OF APPENDIX
Appendix 1 shows the structural coordinates for the solved crystal 3D structure of the TY145 subtilase (SEQ ID NO: 1).



BRIEF DESCRIPTION OF DRAWINGS
FIG. 1 shows a multiple alignment of 3D sequences of subtilases from TY145 (SEQ ID NO: 1), TA39 (SEQ ID NO: 2), TA41 (SEQ ID NO: 3), Bacillus sphaericus (SEQ ID NO: 4) and Savinase (SEQ ID NO: 6).
FIG. 2 shows an alignment between the amino acid sequences of subtilisin BPN′ (SEQ ID NO: 5) and Savinase (SEQ ID NO: 6) in order to define the BPN′ numbering of Savinase.
FIG. 3 shows a superposition of TY145 subtilase (SEQ ID NO: 1) (light) and BPN′ structures (SEQ ID NO: 5) (dark), with spheres indicating ion-binding sites. The TY145 ion-binding sites are light and the BPN′ ion-binding sites are dark.



DEFINITIONS
Prior to discussing this invention in further detail, the following terms and conventions will first be defined.
For a detailed description of the nomenclature of amino acids and nucleic acids, we refer to WO 00/71691 page 5, hereby incorporated by reference. A description of the nomenclature of modifications introduced in a polypeptide by genetic manipulation can be found in WO 00/71691 page 7-12, hereby incorporated by reference.
The term “subtilases” refer to a sub-group of serine protease according to Siezen et al., Protein Engng. 4 (1991) 719-737 and Siezen et al. Protein Science 6 (1997) 501-523. Serine proteases or serine peptidases is a subgroup of proteases characterized by having a serine in the active site, which forms a covalent adduct with the substrate. Further the subtilases (and the serine proteases) are characterized by having two active site amino acid residues apart from the serine, namely a histidine and an aspartic acid residue.
Subtilases are defined by homology analysis of more than 170 amino acid sequences of serine proteases previously referred to as subtilisin-like proteases. The subtilases may be divided into 6 sub-divisions, i.e. the Subtilisin family, the Thermitase family, the Proteinase K family, the Lantibiotic peptidase family, the Kexin family and the Pyrolysin family.
The Subtilisin family (EC 3.4.21.62) may be further divided into 3 sub-groups, i.e. I-S1 (“true” subtilisins), I-S2 (highly alkaline proteases) and intracellular subtilisins. Definitions or grouping of enzymes may vary or change, however, in the context of the present invention the above division of subtilases into sub-division or sub-groups shall be understood as those described by Siezen et al., Protein Engng. 4 (1991) 719-737 and Siezen et al. Protein Science 6 (1997) 501-523.
The term “parent” is in the context of the present invention to be understood as a protein, which is modified to create a protein variant. The parent protein may be a naturally occurring (wild-type) polypeptide or it may be a variant thereof prepared by any suitable means. For instance, the parent protein may be a variant of a naturally occurring protein which has been modified by substitution, chemical modification, deletion or truncation of one or more amino acid residues, or by addition or insertion of one or more amino acid residues to the amino acid sequence, of a naturally-occurring polypeptide. Thus the term “parent subtilase” refers to a subtilase which is modified to create a subtilase variant.
The term “variant” is in the context of the present invention to be understood as a protein which has been modified as compared to a parent protein at one or more amino acid residues.
The term “modification(s)” or “modified” is in the context of the present invention to be understood as to include chemical modification of a protein as well as genetic manipulation of the DNA encoding a protein. The modification(s) may be replacement(s) of the amino acid side chain(s), substitution(s), deletion(s) and/or insertions in or at the amino acid(s) of interest. Thus the term “modified protein”, e.g. “modified subtilase”, is to be understood as a protein which contains modification(s) compared to a parent protein, e.g. subtilase.
The term “(a) TY145 subtilase” or “(a) TY145 like subtilase” should in the context of the present invention be understood as a subtilase belonging to the Subtilisin group according to Siezen et al. Protein Science 6 (1997) 501-523 and which has at least 63% homology to TY145, SEQ ID NO: 1. In the context of the present invention a TY145 subtilase has three ion-binding sites.
The term “(a) BPN′ subtilase” or “(a) BPN′ like subtilase” should in the context of the present invention be understood as a subtilase belonging to the Subtilisin group according Siezen et al. Siezen et al. Protein Science 6 (1997) 501-523 and which has at least 61% homology to BPN′ SEQ ID NO: 5. Such a BPN′ like subtilase is for example Savinase. In the context of the present invention a BPN′ subtilase has two, three or five ion-binding sites. A BPN′ like subtilase may, in the context of the present invention, belong to branch I-S of the subtilisins i.e. to branch I-S1, the “true” subtilisins or I-S2, the highly alkaline proteases (Siezen et al., Protein Engng. 4 (1991) 719-737).
“Homology” or “homologous to” is in the context of the present invention to be understood in its conventional meaning and the “homology” between two amino acid sequences should be determined by use of the “Similarity” defined by the GAP program from the University of Wisconsin Genetics Computer Group (UWGCG) package using default settings for alignment parameters, comparison matrix, gap and gap extension penalties. Default values for GAP penalties, i.e. GAP creation penalty of 3.0 and GAP extension penalty of 0.1 (Program Manual for the Wisconsin Package, Version 8, August 1994, Genetics Computer Group, 575 Science Drive, Madison, Wis., USA 53711). The method is also described in S. B. Needleman and C. D. Wunsch, Journal of Molecular Biology, 48, 443-445 (1970). Identities can be extracted from the same calculation. The homology between two amino acid sequences can also be determined by “identity” or “similarity” using the GAP routine of the UWGCG package version 9.1 with default setting for alignment parameters, comparison matrix, gap and gap extension penalties can also be applied using the following parameters: gap creation penalty=8 and gap extension penalty=8 and all other parameters kept at their default values. The output from the routine is besides the amino acid alignment the calculation of the “Percent Identity” and the “Similarity” between the two sequences. The numbers calculated using UWGCG package version 9.1 is slightly different from the version 8.
The term “position” is in the context of the present invention to be understood as the number of an amino acid in a peptide or polypeptide when counting from the N-terminal end of said peptide/polypeptide. The position numbers used in the present invention refer to different subtilases depending on which subgroup the subtilase belongs to.
The four known subtilases belonging to the TY145 subgroup, i.e. subtilases obtained from TY145, TA39, TA41 and Bacillus sphaericus are numbered individually according to each of SEQ ID NOS: 1, 2, 3 and 4.
Likewise other subtilases belonging to the TY145 subgroup are numbered individually according to their own sequence. However in order to determine homologous positions in such other subtilases an alignment with each of SEQ ID NOS: 1, 2, 3 and 4 is conducted according to the GAP procedure described above. Subsequently the homologous positions are determined with reference to the most homologous of SEQ ID NOS: 1, 2, 3 and 4.
Alternatively subtilases belonging to the TY145 subgroup can be numbered by reference to the positions of TY145 subtilase (SEQ ID NO: 1).
Subtilases belonging to the BPN′ subgroup refers to the positions of Subtilisin Novo (BPN′) from B. amyloliquefaciens (SEQ ID NO: 5).
DETAILED DESCRIPTION OF THE INVENTION
Despite the great homology of the subtilases described above the inventors of the present invention have elucidated the three-dimensional structure of TY145, SEQ ID NO: 1 by X-ray crystallography and found that there are several substantial differences between the three dimensional structures of TY145 and BPN′. The inventors of the present invention have further compared the sequence homology of a representative number of subtilases belonging to the Subtilisin subgroup. This is shown in the homology matrix in Table 1 below.






















TABLE 1





No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15






























1
100
93
76
51
50
51
55
52
54
58
58
59
57
60
60


2

100
75
52
52
52
56
53
55
58
58
61
58
62
61


3


100
60
60
60
58
60
62
58
57
59
59
62
59


4



100
99
99
97
91
76
63
69
74
66
74
74


5




100
99
97
90
76
69
74
66
74
74
56


6





100
98
91
77
63
69
74
66
74
74


7






100
88
79
69
67
74
74
74
74


8







100
77
66
71
74
67
74
74


9








100
64
69
74
67
73
73


10









100
99
76
72
76
76


11










100
76
76
76
76


12











100
99
99
99


13












100
99
99


14













100
98


15














100





Legend to Table 1


TY145 like subtilases:


1: q45681; Subtilase derived from B. subtilis (BSTA41)


2: p28842; Psychrophilic subtilisin derived from Antarctic Bacillus strain (BSTA39)


3: abb77095; Subtilase derived from Bacillus sp. (TY145)


BPN′ like subtilases, I-S1:


4: p00783; Subtilase derived from Bacillus subtilis var. amylosacchariticus (BSAMY)


5: p29142; Subtilase derived from Bacillus stearothermophilus (BSSJ)


6: p35835; Subtilase derived from Bacillus subtilis var. natto. (BSNAT)


7: p07518; Subtilase derived from Bacillus pumilus (B. mesentericus) (BPMES)


8: p00782; Subtilase derived from Bacillus amyloliquefaciens (BPN′)


9: p00780; Subtilase derived from Bacillus licheniformis (BLSCAR)


BPN′ like subtilases, I-S2


10: p41363; Subtilase derived from Bacillus halodurans (BHSAH)


11: aaw62222; Subtilase derived from Bacillus lentus (BLS147)


12: p29600; Subtilase derived from Bacillus lentus (BLSAVI, BLS309)


13: p27693; Subtilase derived from Bacillus alcalophilus (BAALKP)


14: q99405; Subtilase derived from Bacillus sp. strain KSM-K16 (BSKSMK)


15: p29599; Subtilase derived from Bacillus lentus (BLSUBL).






On the basis of the 3D structure comparison and protein sequence the inventors of the present invention find that the subgroup of TY145 subtilases are different from BPN′ subtilases based on the 3D structure comparison of the enclosed 3D structure of TY145 and the BPN′ 3D structure but also indicated from the sequence homology between TY145 and BPN′.
TY145 Subtilases
As described above a TY145 subtilase is in the context of the present invention to be understood as a subtilase which has at least 63% homology to SEQ ID NO: 1. In particular said TY145 subtilase may have at least 65%, such as at least 70%, at least 74%, at least 80%, at least 83%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to TY145, i.e. to SEQ ID NO: 1.
In a first embodiment of the present invention a TY145 subtilase suitable for the purpose described herein may be a subtilase homologous to the three-dimensional structure of TY145, i.e. it may be homologous to the three-dimensional structure defined by the structure coordinates in Appendix 1.
As it is well-known to a person skilled in the art that a set of structure coordinates for a protein or a portion thereof is a relative set of points that define a shape in three dimensions, it is possible that an entirely different set of coordinates could define an identical or a similar shape. Moreover, slight variations in the individual coordinates may have little or no effect on the overall shape.
These variations in coordinates may be generated because of mathematical manipulations of the structure coordinates. For example, the structure coordinates of Appendix 1 (TY145 structure) may be manipulated by crystallographic permutations of the structure coordinates, fractionalization of the structure coordinates, integer additions or subtractions to sets of the structure coordinates, inversion of the structure coordinates or any combination of the above. Alternatively, said variations may be due to differences in the primary amino acid sequence.
If such variations are within an acceptable standard error as compared to the structure coordinates of Appendix 1 said three-dimensional structure is within the context of the present invention to be understood as being homologous to the structure of Appendix 1. The standard error may typically be measured as the root mean square deviation of e.g. conserved backbone residues, where the term “root mean square deviation” (RMS) means the square root of the arithmetic mean of the squares of the deviations from the mean.
As it is also well-known to a person skilled in the art that within a group of proteins which have a homologous structure there may be variations in the three-dimensional structure in certain areas or domains of the structure, e.g. loops, which are not or at least only of a small importance to the functional domains of the structure, but which may result in a big root mean square deviation of the conserved residue backbone atoms between said structures.
Thus it is well known that a set of structure coordinates is unique to the crystallized protein. No other three dimensional structure will have the exact same set of coordinates, be it a homologous structure or even the same protein crystallized in different manner. There are natural fluctuations in the coordinates. The overall structure and the inter-atomic relationship can be found to be similar. The similarity can be discussed in terms of root mean square deviation of each atom of a structure from each “homologous” atom of another structure. However, only identical proteins have the exact same number of atoms. Therefore, proteins having a similarity below 100% will normally have a different number of atoms, and thus the root mean square deviation can not be calculated on all atoms, but only the ones that are considered “homologous”. A precise description of the similarity based on the coordinates is thus difficult to describe and difficult to compute for homologous proteins. Regarding the present invention, similarities in 3D structure of different subtilases can be described by the content of homologous structural elements, and/or the similarity in amino acid or DNA sequence. For sequences having no deletions or insertions a RMS for the calcium atoms can be calculated.
Examples of TY145 like subtilases include the psychrophilic subtilisin protease S41 derived from the Antarctic Bacillus TA41, herein also called TA41 subtilase (Davail S et al., 1994, J. Biol. Chem., 269, 17448-17453), and the psychrophilic subtilisin protease S39 derived from the Antarctic Bacillus TA39, herein also called TA39 subtilase (Narinx E et al., 1997, Protein Engineering, 10 (11), 1271-1279). Recently a three-dimensional structure of a subtilisin homologous with the TY145 subtilisins was published in the Protein Data Bank (Accession No:1EA7). The overall structure and many details of this Bacillus sphaericus “sphericase” subtilase are very homologous with the TY145 subtilase structure; however the structure of the sphericase revealed as much as five ion-binding sites. The number of ion-binding sites may vary in similar structures depending on the medium used for crystallization. Thus it appears that the two extra ion-binding sites of Bacillus sphaericus “sphericase” are due to a calcium containing crystallization medium.
Accordingly, a preferred embodiment of the present invention is a parent subtilase or a subtilase variant which is at least 63% homologous to the sequence of SEQ ID NO: 1, preferably at least 65%, at least 70%, at least 74%, at least 80%, at least 83%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homologous to the sequence of SEQ ID NO: 1, and optionally said subtilase further comprises the following structural characteristics:
a) a twisted beta-sheet with 7 strands,
b) six alpha helices,
c) at least three ion-binding sites and
wherein the Strong ion-binding site of the BPN′ like subtilases is not present, and with the exception of the TY145 subtilase, the TA39 subtilase, the TA41 subtilase, and the Bacillus sphaericus “sphericase”.
The TY145 subtilase of the present invention is encoded by an isolated nucleic acid sequence, which nucleic acid sequence has at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% homology with the nucleic acid sequence shown in SEQ ID NO: 20.
Further the isolated nucleic acid sequence encoding a TY145 subtilase of the invention hybridizes with a complementary strand of the nucleic acid sequence shown in SEQ ID NO: 20 preferably under low stringency conditions, at least under medium stringency conditions, at least under medium/high stringency conditions, at least under high stringency conditions, at least under very high stringency conditions.
Suitable experimental conditions for determining hybridization at *low, medium, or high stringency between a nucleotide probe and a homologous DNA or RNA sequence involves presoaking of the filter containing the DNA fragments or RNA to hybridize in 5×SSC (Sodium chloride/Sodium citrate, Sambrook et al. 1989) for 10 min, and prehybridization of the filter in a solution of 5×SSC, 5×Denhardt's solution (Sambrook et al. 1989), 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA (Sambrook et al. 1989), followed by hybridization in the same solution containing a concentration of 10 ng/ml of a random-primed (Feinberg, A. P. and Vogelstein, B. (1983) Anal. Biochem. 132:6-13), 32P-dCTP-labeled (specific activity>1×109 cpm/μg) probe for 12 hours at ca. 45° C. The filter is then washed twice for 30 minutes in 2×SSC, 0.5% SDS at least * 55° C. (low stringency), more preferably at least 60° C. (medium stringency), still more preferably at least 65° C. (medium/high stringency), even more preferably at least 70° C. (high stringency), and even more preferably at least 75° C. (very high stringency).
BPN′ Subtilases
As described above a BPN′ subtilase is in the context of the present invention to be understood as a subtilase which has at least 61% homology to SEQ ID NO: 5. In particular said BPN′ subtilase may have at least 70%, such as at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to BPN′, i.e. to SEQ ID NO: 5.
In one embodiment of the present invention a BPN′ subtilase suitable for the purpose described herein may be a subtilase homologous to the three-dimensional structure of BPN′ as defined by the structure coordinates given in PDB Nos. 1 SBT and 1 GNS (Protein Data Bank), or one of the several other structures of BPN′ that are accessible from the Protein Data Bank. Variations between homologous structures may occur for several reasons as described above. Thus a BPN′ subtilase within the context of the present invention is to be understood as any subtilase having the structural characteristics pertaining to the BPN′ subtilases as described above, and in addition such subtilases does preferably not have further structural characteristics which are not present in the BPN′ subtilases as described herein. Further a BPN′ subtilase of the present invention may have the necessary percentage of similarity with SEQ ID NO: 5.
Examples of BPN′ like subtilases include the subtilisin 309 (PDB NO:1SVN SAVINASE®, Novozymes A/S) and subtilisin Carlsberg (ALCALASE®, Novozymes A/S), among others.
In FIG. 1 of R. J. Siezen and J. A. M Leunissen (Protein science, Vol. 6 (3), pp. 501-523, 1997) page 502 a structure of subtilases is described. A subtilase consists of 6-8 helices, 11 strands of which 7 are central in a twisted beta-sheet. Two ion-binding sites are mentioned, one of which is the so called “Weak” calcium-binding site. It was later discovered that for some structures (subtilisin DY PDB no. 1BH6, 1998), this calcium-binding site was shown to be a Na (sodium) binding site when the calcium concentration in the crystallization medium was low. Thus, in the following we refer to ion-binding sites instead of calcium-binding sites.
The BPN′ subtilase of the present invention is encoded by an isolated nucleic acid sequence, which nucleic acid sequence has at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% homology with the nucleic acid sequence shown in SEQ ID NO: 21.
Further the isolated nucleic acid sequence encoding a BPN′ subtilase of the invention hybridizes with a complementary strand of the nucleic acid sequence shown in SEQ ID NO: 21 preferably under low stringency conditions, but at least under medium stringency conditions, at least under medium/high stringency conditions, at least under high stringency conditions, at least under very high stringency conditions.
Three-Dimensional Structure of TY145 Subtilases
The TY145 subtilase was used to elucidate the three-dimensional structure forming the basis for the present invention.
The structure of TY145 was solved in accordance with the principle for x-ray crystallographic methods, for example, as given in X-Ray Structure Determination, Stout, G. K. and Jensen, L. H., John Wiley & Sons, Inc. NY, 1989.
The structural coordinates for the solved crystal structure of TY145 are given in standard PDB format (Protein Data Bank, Brookhaven National Laboratory, Brookhaven, Conn.) as set forth in Appendix 1. It is to be understood that Appendix 1 forms part of the present application. In the context of Appendix 1, the following abbreviations are used: CA refers to c-alpha (carbon atoms) or to calcium ions, (however to avoid misunderstandings we use the full names “c-alpha atoms” and “calcium” or “ion” in the present specification). Amino acid residues are given in their standard three-letter code. The attached structural coordinates contain the protease structure, and an inhibitor structure CI2 as well as water molecules. The protease coordinates has a chain identification called A, whereas the CI2 inhibitor is called B, the calcium ions are called C, and the water is W. In the following the positions of the mentioned residues refer to the sequence of TY145 as disclosed in SEQ ID NO: 1.
The structure of TY145 shows the same “overall” fold as found in the S8 family of subtilisins. The structure comprises a twisted beta-sheet with 7 strands arranged in the following sequential order S2, S3, S1, S4, S5, S6, S7. There are six alpha helices in the structure of which number H1 contains residues 9-15, H2 contains residues 72-81, H3 contains residues 114-131, H4 contains residues 148-158, H5 contains residues 250-267 and H6 contains residues 273-286.
The TY145 like subtilases are shown to lack the well-known Strong ion-binding site of the BPN′ subtilases. However, in addition to the Weak calcium or ion-binding site also known from the BPN′ subtilases, the TY145 subtilases have two ion-binding sites which are not present in the BPN′ subtilisin structures. This can be seen in the structural alignment presented in FIG. 3. These additional ion-binding sites are hereinafter referred to as “Near” and “Far” according to their distance to the Weak ion-binding site. Thus in relation to the atomic coordinates disclosed in Appendix 1, the ion-binding sites of TY145 are located at:
Weak—calcium atom named C 314,
Near—calcium atom named C 312, and
Far—calcium atom named C 313 in the PDB table (Appendix 1).
The position of an ion-binding site can be defined by the distance to four specific atoms in the core structure. The distance from the ion-binding site to the c-alpha atoms of the three active site residues has been chosen. Throughout the subtilases the residues Ser, His and Asp in the active site are highly conserved. In TY145 they are Asp35, His72 and Ser251. The fourth distance chosen is the distance to the c-alpha atom of the amino acid residue coming first after the active site serine residue in the sequence (herein after called “next to Ser”); in the 3D structure of TY145 it is Met252.
In a preferred embodiment of the present invention, the distance between:
a) the Weak ion-binding site and i) Asp c-alpha atom is 17.50-19.50 Λ, ii) His c-alpha atom is 21-23 Å, iii) Ser c-alpha atom is 13.80-15.80 Å, iv) next to Ser c-alpha atom is 15.80-17.80 Å,
b) the Far ion-binding site and i) Asp c-alpha atom is 28.70-30.70 Å, ii) His c-alpha atom is 28-30 Å, iii) Ser c-alpha atom is 20-22 Å, iv) next to Ser c-alpha atom is 19.50-21.50 Å,
c) the Near ion-binding site and i) Asp c-alpha atom is 27-29 Å, ii) His c-alpha atom is 29.50-31.50 Å, iii) Ser c-alpha atom is 21.40-23.40 Å, iv) next to Ser c-alpha atom is 22.50-24.50 Å.
Below are the specific distances between the four chosen c-alpha atoms and the three ion binding sites of the TY145 subtilase given in Å:














Weak ion-
Far ion-
Near ion-



binding site 
binding site
binding site


















Met252 c-alpha atom
16.75
20.35
23.58


His72 c-alpha atom
21.98
29.10
30.43


Asp35 c-alpha atom
18.55
29.68
28.04


Ser251 c-alpha atom
14.71
20.96
22.28


Weak ion-binding site
0
16.62
9.79


Far ion-binding site
16.62
0
12.48


Near ion-binding site
9.79
12.48
0









However these distances may vary from one subtilase to the other, and as described above, the Weak ion binding site may also bind to a sodium ion. The present distances are given with a calcium ion in the structure. If a sodium ion was bound instead the distances would be shifted a little bit. Generally the distances can vary ±0.8 Å, preferably ±0.7 Å, ±0.6 Å, ±0.5 Å, ±0.4 Å, or most preferably ±0.3 Å.
Further, in the TY145 like subtilases, the peptide structure circumscribing the Weak ion-binding site is composed of the amino acid residues placed in positions 182-189 and 221-227 with the coordinating atoms being the backbone carbonyl oxygen atom of residues G182, A187, L184 and two water molecules.
The peptide structure circumscribing the Near ion-binding site is composed of residues 212-225 with the coordinating atoms being the backbone carbonyl oxygen atom of residues I220 and T215, the oxygens from the carboxylic acids of residues D225 and D218 and the amid group of residue Q222.
The peptide structure circumscribing the Far ion-binding site is composed of residues 288-306 with the coordinating atoms being the backbone carbonyl oxygen atom of residues G298, G296 and I289, the oxygens from the carboxylic acids of residues D300 and D288, and two water molecules.
In comparison with the BPN′ like subtilase structures the structure of the TY145 like subtilase can be divided into a “common subtilase-like” region, an “intermediate” region and a “nonhomolo-gous” region.
The active site can be found in the common subtilase-like region, which is structurally closely related to the BPN′ structures. The common subtilase-like region is composed of residues 88-128 and 225-284, and contains the alpha-helix H3 and the central alpha-helix H5 in which the active site serine residue is situated in the N-terminal part. The common subtilase-like region has an RMS lower than 1.2.
Outside the common subtilase-like region the structure of the TY145 like subtilase differs from the BPN′ structures to a greater extent.
The intermediate region consist of residues 24-45, 48-58, 65-66, 67-85, 134-174, 175-196, 202-212 and 287-290. The intermediate region has an RMS higher than 1.2 and lower than 1.8. The relationships between the three-dimensional structure and functionality are potentially difficult to predict in this region of the TY145 like subtilases.
The nonhomologous region consists of residues 5-15, 16-23, 86-87, 129-133, 197-201, 213-124, 285-286, 291-298 and 299-311. The nonhomologous region has a RMS higher than 1.5, which also pertains to residues 65-66 from the intermediate region. The group comprising residues 5-15 and 299-311 has an RMS between 2.1-2.2. The relationships between the three-dimensional structure and functionality are very difficult to predict in this region of the TY145 like subtilases.
The regions in areas A1-T5, N16-T24, A46-Q51, S58-C66, G84-G90, S129-K134, S129-K134, S173-S175, V196-T201, N212-R224, A284-V286, K290-D299 and V310-K311 in the TY145 structure differs significantly from the other S8 family subtilisins (including the BPN′ type subtilisins) in c-alpha atom coordinates. An RMS cannot be calculated for these last residues as there are no homologous c-alpha atoms in the compared subtilases.
Homology Building of TY145 and BPN′ Like Subtilases
A model structure of a TY145 like subtilase or a BPN′ like subtilase can be built using the Homology program or a comparable program, e.g., Modeller (both from Molecular Simulations, Inc., San Diego, Calif.). The principle is to align the amino acid sequence of a protein for which the 3D structure is known with the amino acid sequence of a protein for which a model 3D structure has to be constructed. The structurally conserved regions can then be built on the basis of consensus sequences. In areas lacking homology, loop structures can be inserted, or sequences can be deleted with subsequent bonding of the necessary residues using, e.g., the program Homology. Subsequent relaxing and optimization of the structure should be done using either Homology or another molecular simulation program, e.g., CHARMm from Molecular Simulations.
Methods for Designing TY145 and Subtilisin Family Subtilase Variants
Comparisons of the molecular dynamics of different proteins can give a hint as to which domains are important or connected to certain properties pertained by each protein.
The present invention comprises a method of producing a variant of a parent TY145 like subtilase, the variant having at least one altered property as compared to the parent TY145 like subtilase, the method comprising:
a) modelling the parent TY145 subtilase on the three-dimensional structure of a TY145 subtilase to produce a three-dimensional structure of the parent TY145 subtilase;
b) comparing the three-dimensional structure obtained in step a) to the three-dimensional structure of a TY145 subtilase;
c) identifying on the basis of the comparison in step b) at least one structural part of the parent TY145 subtilase, wherein an alteration in said structural part is predicted to result in an altered property;
d) modifying the nucleic acid sequence encoding the parent TY145 subtilase to produce a nucleic acid sequence encoding deletion or substitution of one or more amino acids at a position corresponding to said structural part, or an insertion of one or more amino acid residues in positions corresponding to said structural part;
e) expressing the modified nucleic acid sequence in a host cell to produce the variant TY145 subtilase;
f) isolating the produced subtilase;
g) purifying the isolated subtilase; and
h) recovering the purified subtilase.
Further the present invention comprises a method of producing a variant of a parent Subtilisin family subtilase, such as a BPN′ like subtilase, the variant having at least one altered property as compared to the parent Subtilisin family subtilase, the method comprising:
a) modelling the parent Subtilisin family subtilase on the three-dimensional structure of a Subtilisin family subtilase to produce a three-dimensional structure of the parent Subtilisin family subtilase;
b) comparing the three-dimensional structure obtained in step a) to the three-dimensional structure of a TY145 like subtilase;
c) identifying on the basis of the comparison in step b) at least one structural part of the parent Subtilisin family subtilase, wherein an alteration in said structural part is predicted to result in an altered property;
d) modifying the nucleic acid sequence encoding the parent Subtilisin family subtilase to produce a nucleic acid sequence encoding deletion or substitution of one or more amino acids at a position corresponding to said structural part, or an insertion of one or more amino acid residues in positions corresponding to said structural part;
e) expressing the modified nucleic acid sequence in a host cell to produce the variant Subtilisin family subtilase,
f) isolating the produced subtilase,
g) purifying the isolated subtilase, and
h) recovering the purified subtilase.
Further the present invention comprises a method of producing a variant of a parent TY145 like subtilase, the variant having at least one altered property as compared to the parent TY145 like subtilase, the method comprising:
a) modelling the parent TY145 like subtilase on the three-dimensional structure of a TY145 like subtilase to produce a three-dimensional structure of the parent TY145 like subtilase;
b) comparing the three-dimensional structure obtained in step a) to the three-dimensional structure of a Subtilisin family subtilase;
c) identifying on the basis of the comparison in step b) at least one structural part of the parent TY145 like subtilase, wherein an alteration in said structural part is predicted to result in an altered property;
d) modifying the nucleic acid sequence encoding the parent TY145 like subtilase to produce a nucleic acid sequence encoding deletion or substitution of one or more amino acids at a position corresponding to said structural part, or an insertion of one or more amino acid residues in positions corresponding to said structural part;
e) expressing the modified nucleic acid sequence in a host cell to produce the variant TY145 like subtilase;
f) isolating the produced subtilase;
g) purifying the isolated subtilase; and
h) recovering the purified subtilase.
Stability—Alteration of Ion-Binding Sites
As described above the TY145 subtilases has two new ion-binding sites not present in the BPN′ subtilisin structures but lacks the Strong ion-binding site of the BPN′ subtilases. Stability of the ion-binding site is of crucial importance for the functionality of the enzyme. Therefore alterations of the amino acid residues close to the ion-binding sites are likely to result in alterations of the stability of the enzyme.
The positions which may be modified are located:
Weak: at a distance of 10 Å or less around calcium atom named C 314,
Near: at a distance of 10 Å or less around calcium atom named C 312, and
Far: at a distance of 10 Å or less around calcium atom named C 313 in the PDB table (Appendix 1).
Improved Stability
Stabilization of the ion-binding sites of TY145 may possibly be obtained by alterations in the positions close to the sites. Positions located at a distance of 10 Å or less to the ion-binding sites of TY145 (SEQ ID NO: 1) are:
Weak: 154, 155, 158, 164, 165, 166, 167, 168, 178-191 (i.e. 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191), 211, 220-228 (i.e. 220, 221, 222, 223, 224, 225, 226, 227, 228), 277, 281 and 305.
Near: 185, 211-227 (i.e. 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227), 277, 281, 299, 300, 301, 304, 305.
Far: 193, 198, 199, 201, 202, 204, 216, 217, 219, 226, 227, 228, 229 and 284-307 (i.e. 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307).
In detergent compositions calcium chelaters contribute to removal of calcium from the subtilases with subsequent inactivation of the enzyme as the result. To decrease the inactivation due to calcium removal of e.g. calcium chelaters, variants with improved calcium stability can be constructed.
Variants with alterations close to the Near ion-binding site are I220S,T and T215S, variants with alterations close to the Far ion-binding site are G298A,S,T and G296A,S,T, and variants with alterations close to the Weak ion-binding site are V185T and I221N,D,T.
TY145 with Extra Ion-Binding Site
The Strong ion-binding site from the BPN′ subtilases can be transplanted into TY145 (or other subtilases in TY145 subgroup) by deletion(s) of or in the region H83-G90 (of SEQ ID NO: 1) and subsequent insertion of one or more amino acid residues. A preferred variant has the whole region deleted and a subsequent insertion between A82 and V91 of the sequence LNNSIG.
Removal of Ion-Binding Site in TY145
By removing a ion-binding site it is possible to alter the enzymes dependency of calcium or other ions in the solution. The Far and Near ion-binding sites in TY145 (or others from TY145 group) can be removed with guidance from the three-dimensional structure of BPN′ and Savinase (or others in BPN′ group).
Removal of the Far site can be done by deletion(s) of or in the region K290-D300 (of SEQ ID NO: 1) and subsequent insertion of one or more amino acid residues. A preferred variant has the whole region deleted and a subsequent insertion between I289 and Y301 of the sequence GDS or DST. Preferably, but not mandatory the substitution S303Y is further added.
Removal of the Near site can be done by deletion(s) of or in the region N212-R224 (of SEQ ID NO: 1) and subsequent insertion of one or more amino acid residues. A preferred variant has the whole region deleted and a subsequent insertion of a proline or alanine residue between G211 and D225.
Removal of Strong Ion-Binding Site in BPN′ Subtilases
The Strong ion-binding site in BPN′ like subtilases can be removed. Exemplified in Savinase, the removal can be done by deletion of or in the region L75-G80 (BPN′ numbering) and subsequent insertion of one or more amino acid residues. A preferred variant has the whole region deleted and a subsequent insertion of residues 84-88 from TY145. In addition the substitutions L82Y and Q2A,N can be applied.
Alteration of Thermostability
A variant with improved stability (typically increased thermostability) may be obtained by substitution with proline, introduction of a disulfide bond, altering a hydrogen bond contact, altering charge distribution, introduction of a salt bridge, filling in an internal structural cavity with one or more amino acids with bulkier side groups (in e.g. regions which are structurally mobile), substitution of histidine residues with other amino acids, removal of a deamidation site, or by helix capping.
Regions with Increased Mobility:
The following regions of TY145 have an increased mobility in the crystal structure of the enzyme, and it is presently believed that these regions can be responsible for stability or activity of TY145. Especially thermostabilization may possibly be obtained by altering the highly mobile regions. Improvements of the enzyme can be obtained by mutation in the below regions and positions. Introducing e.g. larger residues or residues having more atoms in the side chain could increase the stability, or, e.g., introduction of residues having fewer atoms in the side chain could be important for the mobility and thus the activity profile of the enzyme. The regions can be found by analysing the B-factors taken from the coordinate file in Appendix 1, and/or from molecular dynamics calculations of the isotropic fluctuations. These can be obtained by using the program CHARMm from MSI (Molecular Simulations Inc.).
Molecular dynamics simulation at 300K of TY145 reveals the following highly mobile regions:
84-89 (i.e. 84, 85, 86, 87, 88, 89)
108-117 (i.e. 108, 109, 110, 111, 112, 113, 114, 115, 116, 117)
141-146 (i.e. 141, 142, 143, 144, 145, 146)
150-152 (i.e. 150, 151, 152)
169-171 (i.e. 169, 170, 171)
200-201
211-220 (i.e. 211, 212, 213, 214, 215, 216, 217, 218, 219, 220)
242-243
268-270 (i.e. 268, 269, 270).
Also B-factors (see “in X-Ray Structure Determination, Stout, G. K. and Jensen, L. H., John Wiley & Sons, Inc. NY, 1989”) from crystallographic data indicates the following more mobile regions in the TY145 structure:
1-7 (i.e. 1, 2, 3, 4, 5, 6, 7),
17-23 (i.e. 17, 18, 19, 20, 21, 22, 23),
38-50 (i.e. 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50),
57-69 (i.e. 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69),
84-92 (i.e. 84, 85, 86, 87, 88, 89, 90, 91, 92),
107-110 (i.e. 107, 108, 109, 110),
239-243 (i.e. 239, 240, 241, 242, 243) and
265-266.
Preferably the regions 57-69 and 84-92.
Disulfide Bonds:
A TY145 variant of the present invention with improved stability, e.g. thermostability, as compared to the parent TY145 may be obtained by introducing new inter-domain or intra-domain bonds, such as by establishing inter- or intra-domain disulfide bridges.
Thus a further aspect of the present invention relates to a method for producing a variant of a parent TY145 comprising the methods described in the paragraph “Methods of preparing TY145 like or BPN′ like subtilase variants” herein.
According to the guidelines mentioned above the below mentioned amino acid residues identified in the amino acid sequence of SEQ ID NO: 1 are suitable for cysteine replacement. With one or more of these substitutions with cysteine, disulfide bridges may possibly form in a variant of TY145. The substitutions are: G26C+A95C; A167C+T254C; R203C+G292C and V228C+A284C.
Similar residues suitable for cysteine replacement in homologous subtilases such as TA39, TA41 can be elucidated by finding the homologous positions in the alignment of FIG. 1. Concerning another TY145 like sequence the homologous positions suitable for cysteine replacement can be selected by aligning said TY145 like sequence with all of the sequences of FIG. 1 using the GAP analysis method as described above. The suitable residues can then be selected in accordance with the homologous positions in the most homologous of SEQ ID NOS: 1, 2, 3 and 4 which are the sequences of the subtilases aligned in FIG. 1.
Surface Charge Distribution
A variant with improved stability (typically improved thermostability) as compared to the parent subtilase may be obtained by changing the surface charge distribution of the subtilase. For example, when the pH is lowered to about 5 or below histidine residues typically become positively charged and, consequently, unfavorable electrostatic interactions on the protein surface may occur. By engineering the surface charge of the subtilase one may avoid such unfavorable electrostatic interactions that in turn lead to a higher stability of the subtilase.
Therefore, a further aspect of the present invention relates to a method for constructing a variant of a parent subtilase, the method comprising:
a) identifying, on the surface of the parent subtilase, preferably a TY145 like or a BPN′ like subtilase, at least one amino acid residue selected from the group consisting of Asp, Glu, Arg, Lys and His;
b) substituting, on the surface of the parent subtilase, at least one amino acid residue selected from the group consisting of Asp, Glu, Arg, Lys and His with an uncharged amino acid residue;
c) optionally repeating steps a) and b) recursively;
d) optionally, making alterations each of which is an insertion, a deletion or a substitution of an amino acid residue at one or more positions other than b);
e) preparing the variant resulting from steps a)-d);
f) testing the stability of said variant; and
g) optionally repeating steps a)-f) recursively; and
h) selecting a subtilase variant having increased stability as compared to the parent subtilase.
As it will be understood by the skilled person it may also, in some cases, be advantageous to substitute an uncharged amino acid residue with an amino acid residue bearing a charge or, alternatively, it may in some cases be advantageous to substitute an amino acid residue bearing a charge with an amino acid residue bearing a charge of opposite sign. Thus, the above-mentioned method may easily be employed by the skilled person also for these purposes. In the case of substituting an uncharged amino acid residue with an amino acid residue bearing a charge the above-mentioned method may be employed the only difference being steps a) and b) which will then read:
a) identifying, on the surface of the parent subtilase, at least one uncharged amino acid residue;
b) substituting, on the surface of the parent subtilase, at least one uncharged amino acid residue with a charged amino acid residue selected from the group consisting of Asp, Glu, Arg, Lys and His.
Also in the case of changing the sign of an amino acid residue present on the surface of the subtilase the above method may be employed. Again, compared to the above method, the only difference being steps a) and b) which, in this case, read:
a) identifying, on the surface of the parent subtilase, at least one charged amino acid residue selected from the group consisting of Asp, Glu, Arg, Lys and His;
b) substituting, on the surface of the parent subtilase, at least one charged amino acid residue identified in step a) with an amino acid residue having an opposite charge.
Thus, Asp may be substituted with Arg, Lys or His; Glu may be substituted with Arg, Lys or His; Arg may be substituted with Asp or Glu; Lys may be substituted with Asp or Glu; and His may be substituted with Asp or Glu.
In order to determine the amino acid residues of a subtilase, which are present on the surface of the enzyme, the surface accessible area are measured using the DSSP program (Kabsch and Sander, Biopolymers (1983), 22, 2577-2637). All residues having a surface accessibility higher than 0 is regarded a surface residue.
An amino acid residue found on the surface of TY145 using the above method is D116 and it is contemplated that the substitutions D116H,K,R are of particular interest.
Similar substitutions may be introduced in equivalent positions of other TY145 like subtilases.
Substitution with Proline Residues
Improved thermostability of a subtilase can be obtained by subjecting the subtilase in question to analysis for secondary structure, identifying residues in the subtilase having dihedral angles φ (phi) and ψ (psi) confined to the intervals [−90°<φ<−40° and −180°<ψ<180°], preferably the intervals [−90°<φ<−40° and 120°<ψ<180] or [−90°<φ<−40° and −50°<ψ<10° ] and excluding residues located in regions in which the subtilase is characterized by possessing α-helical or β-sheet structure.
After the dihedral angles φ (phi) and ψ (psi) for the amino acids have been calculated, based on the atomic structure in the crystalline subtilases, it is possible to select position(s) which has/have dihedral phi and psi angles favourable for substitution with a proline residue. The aliphatic side chain of proline residues is bonded covalently to the nitrogen atom of the peptide group. The resulting cyclic five-membered ring consequently imposes a rigid constraint on the rotation about the N—Cα bond of the peptide backbone and simultaneously prevents the formation of hydrogen bonding to the backbone N-atom. For these structural reasons, proline residues are generally not compatible with α-helical and β-sheet secondary conformations.
If a proline residue is not already at the identified position(s), the naturally occurring amino acid residue is substituted with a proline residue, preferably by site directed mutagenesis applied on a gene encoding the subtilase in question.
In the group of TY145 like subtilases proline residues can be introduced at positions 18, 115, 185, 269 and 293. Accordingly, a preferred TY145 variant has one or more of the substitutions: Q18P, D115P, V185P, T269P and I293P.
Alteration of Activity
Introduction of Activity at Low Temperature in TY145 and Savinase
A comparison of the molecular dynamics at 300K of TY145 (a mesophilic-derived enzyme obtained from crystal structure) and TA41 (a psychrophilic derived enzyme obtained from modelling) was conducted.
The comparison was directed to low temperature activity and revealed a difference in dynamical behaviour of TY145 and TA41. The theory derived from the comparison is that the difference in dynamics, especially around the active site, are important for the low temperature functionality of the psychrophilic enzyme. The necessary dynamics are needed for the enzyme to have activity at low temperature and thus the activity drops if the enzymes dynamics are lowered.
The higher mobility regions in TA41 compared to TY145 measured by molecular dynamics simulation indicates important regions for the low temperature activity, of the enzyme TA41 which can be transferred to TY145.
The regions in TA41 are:

    16-22 (i.e. 16, 17, 18, 19, 20, 21, 22),
    40-73 (i.e. 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73),
    118-131 (i.e. 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131)
    140-161 (i.e. 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161) and
    275-294 (i.e. 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294).


Regions closest to the active site and the substrate binding site are regarded as preferred in relation to making higher activity at low temperature for TY145: 40-73 and 140-161, preferably 65-73 and 140-150. The regions in TY145 should be modified to be more mobile for example by substitution with small less rigid residues, i.e. residues with smaller side chains (such as Gly, Ala, Ser, Thr or Val), into the TY145 backbone.
The other regions in TA41 are most interesting for stabilization of the psychrophilic enzyme. These regions can easily be found in TA39 as well or in other homologous enzymes, also non psychrophilics.
The regions around the active site and the substrate binding site are the regions most likely involved in the low temperature functionality.
Below are suggestions for transferring the low temperature activity of TA41 and homologous sequences to TY145-like sequences and the BPN′-like sequences:












TA41
TY145
Savinase







I31
V31I, A, L
V28I, A, L


V38
V38A, L
I35V, A, L


S79
T79S
T71S


A80
V80A,G, V
172A, G, V


L81
L81G
A73L, G


V187
V188A
M175V, A


T253
T254S, A 
T224S, A









The numbering is according to SEQ ID NOS: 3, 1 and 5 respectively. Savinase is numbered according to subtilisin BPN′.
Preferred Savinase variants are V28I, I35V, T71S, I72A, A73L, M175V and T224S.
Examples of core variants of TY145 are: V31I, V80A, T79S.
The alterations of the TY145-like sequences and the BPN′-like sequences can be single mutations or combinations of the suggested mutations.
Substrate Bindings Site
The substrate binding site is identified by the residues in contact with a substrate model, such as the CI2 inhibitor. The 3D structure coordinates of the TY145 subtilase with CI2 bound in the active site can be found in Appendix 1. Without being limited to any theory, it is presently believed that binding between a substrate and an enzyme is supported by favorable interactions found within a sphere 10 Å from the substrate molecule, in particular within a sphere of 6 Å from the substrate molecule. Examples of such favorable bonds are hydrogen bonds, strong electrostatic interaction and/or hydrophobic interactions.
The following residues of the TY145 subtilase (SEQ ID NO: 1), are within a distance of 6 Å from the CI2 inhibitor and thus believed to be involved in interactions with said substrate:
35, 36, 70, 72, 106, 109, 110, 111, 112, 113, 114, 117, 139, 140, 141, 142, 143, 144, 145, 147, 150, 167, 168, 169, 170, 171, 172, 173, 174, 177, 180, 207, 239, 247, 248, 249, 250, 251 and 252.
Stabilization by Modification of Asn-Gly Pairs
It is known that at alkaline pH, the side chain of Asn may interact with the NH group of a sequential neighbouring amino acid to form an isoAsp residue where the backbone goes through the Asp side chain. This will leave the backbone more vulnerable to proteolysis. The deamidation is much more likely to occur if the residue that follows is a Gly. Changing the Asn in front of the Gly or the Gly will prevent this from happening and thus improve the stability, especially as concerns thermo- and storage stability.
The invention consequently further relates to a subtilase, in which either or both residues of any of the Asn-Gly sequence appearing in the amino acid sequence of the parent RP-II protease is/are deleted or substituted with a residue of a different amino acid.
The Asn and/or Gly residue may, for instance, be substituted with a residue of an amino acid selected from the group consisting of A, Q, S, P, T and Y.
Asn-Gly sequences can be found in the following positions:
B. sphaericus: 198-199, 240-241
TY145: 87-88, 109-110, 199-200
TA41: 83-84, 198-199
TA39: 88-89, 198-199
The present invention in this respect thus relates to modifications, such as deletions and substitutions in one or more of these positions in accordance with the principles given above.
Modification of Tyrosine Residues
In relation to wash performance it has been found that the modification of certain tyrosine residues to phenylalanine provides an improved wash performance. Without being bound by any specific theory, it is believed that titration of these Tyr residues in the alkaline wash liquor has negative effects that are alleviated by replacing the Tyr residues with other residues, especially Phe or Trp, particularly Phe.
Tyrosines can be found in the following positions:
B. sphaericus: 14, 91, 102, 112, 155, 157, 172, 179, 201, 206, 211, 218, 235, 239, 243, 292, 300,
TY145: 15, 39, 92, 103, 113, 156, 158, 202, 219, 240, 244, 287, 301, 307,
TA41: 15, 91, 102, 112, 155, 157, 179, 201, 218, 235, 243,
TA39: 15, 61, 91, 102, 112, 155, 157, 173, 179, 201, 211, 218, 235, 243, 267, 281, 284, 292, 293, 296
The present invention in this respect thus relates to modifications, such as deletions and substitutions in one or more of these positions in accordance with the principles given above.
Modification of Methionine Residues
In order to improve the oxidation stability of proteins it has been found that the substitution or even deletion of methionine residues is beneficial, Especially modification of the methionine residue normally found next to the active serine residue may provide a significant improvement of the oxidation stability. Modifications to Ser or Ala are the most preferred substitutions for this Met.
Methionines can be found in the following positions:
B. sphaericus: 138, 251,
TY145: 139, 252,
TA41: 1, 138, 251,
TA39: 1, 138, 251.
The present invention in this respect thus relates to modifications, such as deletions and substitutions in one or more of these positions in accordance with the principles given above.
Combined Modifications
The present invention also encompasses any of the above mentioned subtilase variants in combination with any other modification to the amino acid sequence thereof. Especially combinations with other modifications known in the art to provide improved properties to the enzyme are envisaged.
Such combinations comprise the positions: 222 (improves oxidation stability), 218 (improves thermal stability), substitutions in the Ca2+-binding sites stabilizing the enzyme, e.g. position 76, and many other apparent from the prior art (all positions according to BPN′ numbering).
In further embodiments a subtilase variant described herein may advantageously be combined with one or more modification(s) in any of the positions: 27, 36, 56, 76, 87, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 120, 123, 159, 167, 170, 206, 218, 222, 224, 232, 235, 236, 245, 248, 252 and 274 (BPN′ numbering).
Specifically, the following BLSAVI, BLSUBL, BSKSMK, and BAALKP modifications are considered appropriate for combination:
K27R, *36D, S56P, N76D, S87N, G97N, S101G, S103A, V104A, V104I, V104N, V104Y, H120D, N123S, G159D, Y167A, R1705, R170L, Q206E, N218S, M222S, M222A, T224S, A232V, K235L, Q236H, Q245R, N248D, N252K and T274A (BPN′ numbering).
Furthermore variants comprising any of the modifications S101G+V104N, S87N+S101G+V104N, K27R+V104Y+N123S+T274A, N76D+S103A+V104I or N76D+V104A, or other combinations of the modifications K27R, N76D, S101G, S103A, V104N, V104Y, V104I, V104A, N123S, G159D, A232V, Q236H, Q245R, N248D, N252K, T274A in combination with any one or more of the modification(s) mentioned above exhibit improved properties.
A particular interesting variant is a variant, which, in addition to modifications according to the invention, contains the following substitutions:
S101G+S103A+V104I+G159D+A232V+Q236H+Q245R+N248D+N252K.
Moreover, subtilase variants of the main aspect(s) of the invention are preferably combined with one or more modification(s) in any of the positions 129, 131 and 194, preferably as 129K, 131H and 194P modifications, and most preferably as P129K, P131H and A194P modifications. Any of those modification(s) are expected to provide a higher expression level of the subtilase variant in the production thereof.
Methods of Preparing TY145 Like or BPN′ Like Subtilase Variants
The subtilase variants, i.e. the TY145 and BPN′ variants of the present invention may be produced by any known method within the art and the present invention also relates to nucleic acid encoding a subtilase variant of the present invention, a DNA construct comprising said nucleic acid and a host cell comprising said nucleic acid sequence.
In general natural occurring proteins may be produced by culturing the organism expressing the protein and subsequently purifying the protein or it may be produced by cloning a nucleic acid, e.g. genomic DNA or cDNA, encoding the protein into an expression vector, introducing said expression vector into a host cell, culturing the host cell and purifying the expressed protein.
Typically protein variants may be produced by site-directed mutagenesis of a parent protein, introduction into expression vector, host cell etc. The parent protein may be cloned from a strain producing the polypeptide or from an expression library, i.e. it may be isolated from genomic DNA or prepared from cDNA, or a combination thereof.
In general standard procedures for cloning of genes and/or introducing mutations (random and/or site directed) into said genes may be used in order to obtain a parent subtilase, or subtilase or subtilase variant of the invention. For further description of suitable techniques reference is made to Molecular cloning: A laboratory manual (Sambrook et al. (1989), Cold Spring Harbor lab., Cold Spring Harbor, N.Y.; Ausubel, F. M. et al. (eds.)); Current protocols in Molecular Biology (John Wiley and Sons, 1995; Harwood, C. R., and Cutting, S. M. (eds.)); Molecular Biological Methods for Bacillus (John Wiley and Sons, 1990); DNA Cloning: A Practical Approach, Volumes I and II (D. N. Glover ed. 1985); Oligonucleotide Synthesis (M. J. Gait ed. 1984); Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds (1985)); Transcription And Translation (B. D. Hames & S. J. Higgins, eds. (1984)); Animal Cell Culture (R. I. Freshney, ed. (1986)); Immobilized Cells And Enzymes (IRL Press, (1986)); A Practical Guide To Molecular Cloning (B. Perbal, (1984)) and WO 96/34946.
Further, variants could be constructed by:
Random Mutagenesis
Random mutagenesis is suitably performed either as localized or region-specific random mutagenesis in at least three parts of the gene translating to the amino acid sequence shown in question, or within the whole gene.
The random mutagenesis of a DNA sequence encoding a parent subtilase may be conveniently performed by use of any method known in the art.
In relation to the above, a further aspect of the present invention relates to a method for generating a variant of a parent subtilase, wherein the variant exhibits an altered property, such as increased thermostability, increased stability at low pH and at low calcium concentration, relative to the parent subtilase, the method comprising:
(a) subjecting a DNA sequence encoding the parent subtilase to random mutagenesis,
(b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and
(c) screening for host cells expressing a subtilase variant which has an altered property relative to the parent subtilase.
Step (a) of the above method of the invention is preferably performed using doped primers.
For instance, the random mutagenesis may be performed by use of a suitable physical or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting the DNA sequence to PCR generated mutagenesis. Furthermore, the random mutagenesis may be performed by use of any combination of these mutagenizing agents. The mutagenizing agent may, e.g., be one which induces transitions, transversions, inversions, scrambling, deletions, and/or insertions.
Examples of a physical or chemical mutagenizing agent suitable for the present purpose include ultraviolet (UV) irradiation, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, and nucleotide analogues. When such agents are used, the mutagenesis is typically performed by incubating the DNA sequence encoding the parent enzyme to be mutagenized in the presence of the mutagenizing agent of choice under suitable conditions for the mutagenesis to take place, and selecting for mutated DNA having the desired properties.
When the mutagenesis is performed by the use of an oligonucleotide, the oligonucleotide may be doped or spiked with the three non-parent nucleotides during the synthesis of the oligonucleotide at the positions that are to be changed. The doping or spiking may be done so that codons for unwanted amino acids are avoided. The doped or spiked oligonucleotide can be incorporated into the DNA encoding the subtilase enzyme by any published technique, using, e.g., PCR, LCR or any DNA polymerase and ligase as deemed appropriate.
Preferably, the doping is carried out using “constant random doping”, in which the percentage of wild-type and modification in each position is predefined. Furthermore, the doping may be directed toward a preference for the introduction of certain nucleotides, and thereby a preference for the introduction of one or more specific amino acid residues. The doping may be made, e.g., so as to allow for the introduction of 90% wild type and 10% modifications in each position. An additional consideration in the choice of a doping scheme is based on genetic as well as protein-structural constraints. The doping scheme may be made by using the DOPE program which, inter alia, ensures that introduction of stop codons is avoided (L. J. Jensen et al. Nucleic Acid Research, 26, 697-702 (1998).
When PCR-generated mutagenesis is used, either a chemically treated or non-treated gene encoding a parent subtilase enzyme is subjected to PCR under conditions that increase the misincorporation of nucleotides (Deshler 1992; Leung et al., Technique, 1, 1989, pp. 11-15).
A mutator strain of E. coli (Fowler et al., Molec. Gen. Genet., 133, 1974, 179-191), S. cereviseae or any other microbial organism may be used for the random mutagenesis of the DNA encoding the subtilase by, e.g., transforming a plasmid containing the parent enzyme into the mutator strain, growing the mutator strain with the plasmid and isolating the mutated plasmid from the mutator strain. The mutated plasmid may be subsequently transformed into the expression organism.
The DNA sequence to be mutagenized may conveniently be present in a genomic or cDNA library prepared from an organism expressing the parent subtilase. Alternatively, the DNA sequence may be present on a suitable vector such as a plasmid or a bacteriophage, which as such may be incubated with or otherwise exposed to the mutagenising agent. The DNA to be mutagenized may also be present in a host cell either by being integrated in the genome of said cell or by being present on a vector harbored in the cell. Finally, the DNA to be mutagenized may be in isolated form. It will be understood that the DNA sequence to be subjected to random mutagenesis is preferably a cDNA or a genomic DNA sequence.
In some cases it may be convenient to amplify the mutated DNA sequence prior to performing the expression step b) or the screening step c). Such amplification may be performed in accordance with methods known in the art, the presently preferred method being PCR-generated amplification using oligonucleotide primers prepared on the basis of the DNA or amino acid sequence of the parent enzyme.
Subsequent to the incubation with or exposure to the mutagenising agent, the mutated DNA is expressed by culturing a suitable host cell carrying the DNA sequence under conditions allowing expression to take place. The host cell used for this purpose may be one which has been transformed with the mutated DNA sequence, optionally present on a vector, or one which was carried the DNA sequence encoding the parent enzyme during the mutagenesis treatment. Examples of suitable host cells are the following: gram positive bacteria such as Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans or Streptomyces murinus; and gram negative bacteria such as E. coli. 
The mutated DNA sequence may further comprise a DNA sequence encoding functions permitting expression of the mutated DNA sequence.
Localized Random Mutagenesis
The random mutagenesis may be advantageously localized to a part of the parent subtilase in question. This may, e.g., be advantageous when certain regions of the enzyme have been identified to be of particular importance for a given property of the enzyme, and when modified are expected to result in a variant having improved properties. Such regions may normally be identified when the tertiary structure of the parent enzyme has been elucidated and related to the function of the enzyme.
The localized or region-specific, random mutagenesis is conveniently performed by use of PCR generated mutagenesis techniques as described above or any other suitable technique known in the art. Alternatively, the DNA sequence encoding the part of the DNA sequence to be modified may be isolated, e.g., by insertion into a suitable vector, and said part may be subsequently subjected to mutagenesis by use of any of the mutagenesis methods discussed above.
General Method for Random Mutagenesis by Use of the DOPE Program
The random mutagenesis may be carried out by the following steps:

    1. Select regions of interest for modification in the parent enzyme
    2. Decide on mutation sites and non-mutated sites in the selected region
    3. Decide on which kind of mutations should be carried out, e.g. with respect to the desired stability and/or performance of the variant to be constructed
    4. Select structurally reasonable mutations
    5. Adjust the residues selected by step 3 with regard to step 4.
    6. Analyse by use of a suitable dope algorithm the nucleotide distribution.
    7. If necessary, adjust the wanted residues to genetic code realism, e.g. taking into account constraints resulting from the genetic code, e.g. in order to avoid introduction of stop codons; the skilled person will be aware that some codon combinations cannot be used in practice and will need to be adapted
    8. Make primers
    9. Perform random mutagenesis by use of the primers
    10. Select resulting subtilase variants by screening for the desired improved properties.


Suitable dope algorithms for use in step 6 are well known in the art. One such algorithm is described by Tomandl, D. et al., 1997, Journal of Computer-Aided Molecular Design 11:29-38. Another algorithm is DOPE (Jensen, L J, Andersen, K V, Svendsen, A, and Kretzschmar, T (1998) Nucleic Acids Research 26:697-702).
Expression Vectors
A recombinant expression vector comprising a nucleic acid sequence encoding a subtilase variant of the invention may be any vector that may conveniently be subjected to recombinant DNA procedures and which may bring about the expression of the nucleic acid sequence.
The choice of vector will often depend on the host cell into which it is to be introduced. Examples of a suitable vector include a linear or closed circular plasmid or a virus. The vector may be an autonomously replicating vector, i.e., a vector which exists as an extra-chromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extra-chromosomal element, a mini chromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Examples of bacterial origins of replication are the origins of replication of plasmids pBR322, pUC19, pACYC177, pACYC184, pUB110, pE194, pTA1060, and pAMβ1. Examples of origin of replications for use in a yeast host cell are the 2 micron origin of replication, the combination of CEN6 and ARS4, and the combination of CEN3 and ARS1. The origin of replication may be one having a mutation which makes it function as temperature-sensitive in the host cell (see, e.g., Ehrlich, 1978, Proceedings of the National Academy of Sciences USA 75:1433).
Alternatively, the vector may be one which, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Vectors which are integrated into the genome of the host cell may contain any nucleic acid sequence enabling integration into the genome, in particular it may contain nucleic acid sequences facilitating integration into the genome by homologous or non-homologous recombination. The vector system may be a single vector, e.g. plasmid or virus, or two or more vectors, e.g. plasmids or virus′, which together contain the total DNA to be introduced into the genome of the host cell, or a transposon.
The vector may in particular be an expression vector in which the DNA sequence encoding the subtilase variant of the invention is operably linked to additional segments or control sequences required for transcription of the DNA. The term, “operably linked” indicates that the segments are arranged so that they function in concert for their intended purposes, e.g. transcription initiates in a promoter and proceeds through the DNA sequence encoding the subtilase variant. Additional segments or control sequences include a promoter, a leader, a polyadenylation sequence, a propeptide sequence, a signal sequence and a transcription terminator. At a minimum the control sequences include a promoter and transcriptional and translational stop signals.
The promoter may be any DNA sequence that shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell.
Examples of suitable promoters for use in bacterial host cells include the promoter of the Bacillus subtilis levansucrase gene (sacB), the Bacillus stearothermophilus maltogenic amylase gene (amyM), the Bacillus licheniformis alpha-amylase gene (amyL), the Bacillus amyloliquefaciens alpha-amylase gene (amyQ), the Bacillus subtilis alkaline protease gene, or the Bacillus pumilus xylosidase gene, the Bacillus amyloliquefaciens BAN amylase gene, the Bacillus licheniformis penicillinase gene (penP), the Bacillus subtilis xylA and xylB genes, and the prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978, Proceedings of the National Academy of Sciences USA 75:3727-3731). Other examples include the phage Lambda PR or PL promoters or the E. coli lac, trp or tac promoters or the Streptomyces coelicolor agarase gene (dagA). Further promoters are described in “Useful proteins from recombinant bacteria” in Scientific American, 1980, 242:74-94; and in Sambrook et al., 1989, supra.
Examples of suitable promoters for use in a filamentous fungal host cell are promoters obtained from the genes encoding Aspergillus oryzae TAKA amylase, Rhizomucor miehei aspartic proteinase, Aspergillus niger neutral alpha-amylase, Aspergillus niger acid stable alpha-amylase, Aspergillus niger or Aspergillus awamori glucoamylase (glaA), Rhizomucor miehei lipase, Aspergillus oryzae alkaline protease, Aspergillus oryzae triose phosphate isomerase, Aspergillus nidulans acetamidase, Fusarium oxysporum trypsin-like protease (as described in U.S. Pat. No. 4,288,627, which is incorporated herein by reference), and hybrids thereof. Particularly preferred promoters for use in filamentous fungal host cells are the TAKA amylase, NA2-tpi (a hybrid of the promoters from the genes encoding Aspergillus niger neutral (-amylase and Aspergillus oryzae triose phosphate isomerase), and glaA promoters. Further suitable promoters for use in filamentous fungus host cells are the ADH3 promoter (McKnight et al., The EMBO J. 4 (1985), 2093-2099) or the tpiA promoter.
Examples of suitable promoters for use in yeast host cells include promoters from yeast glycolytic genes (Hitzeman et al., J. Biol. Chem. 255 (1980), 12073-12080; Alber and Kawasaki, J. Mol. Appl. Gen. 1 (1982), 419-434) or alcohol dehydrogenase genes (Young et al., in Genetic Engineering of Microorganisms for Chemicals (Hollaender et al, eds.), Plenum Press, New York, 1982), or the TPI1 (U.S. Pat. No. 4,599,311) or ADH2-4c (Russell et al., Nature 304 (1983), 652-654) promoters.
Further useful promoters are obtained from the Saccharomyces cerevisiae enolase (ENO-1) gene, the Saccharomyces cerevisiae galactokinase gene (GAL1), the Saccharomyces cerevisiae alcohol dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase genes (ADH2/GAP), and the Saccharomyces cerevisiae 3-phosphoglycerate kinase gene. Other useful promoters for yeast host cells are described by Romanos et al., 1992, Yeast 8:423-488. In a mammalian host cell, useful promoters include viral promoters such as those from Simian Virus 40 (SV40), Rous sarcoma virus (RSV), adenovirus, and bovine papilloma virus (BPV).
Examples of suitable promoters for use in mammalian cells are the SV40 promoter (Subramani et al., Mol. Cell Biol. 1 (1981), 854-864), the MT-1 (metallothionein gene) promoter (Palmiter et al., Science 222 (1983), 809-814) or the adenovirus 2 major late promoter.
An example of a suitable promoter for use in insect cells is the polyhedrin promoter (U.S. Pat. No. 4,745,051; Vasuvedan et al., FEBS Lett. 311, (1992) 7-11), the P10 promoter (J. M. Vlak et al., J. Gen. Virology 69, 1988, pp. 765-776), the Autographa californica polyhedrosis virus basic protein promoter (EP 397 485), the baculovirus immediate early gene 1 promoter (U.S. Pat. No. 5,155,037; U.S. Pat. No. 5,162,222), or the baculovirus 39K delayed-early gene promoter (U.S. Pat. No. 5,155,037; U.S. Pat. No. 5,162,222).
The DNA sequence encoding a subtilase variant of the invention may also, if necessary, be operably connected to a suitable terminator.
The recombinant vector of the invention may further comprise a DNA sequence enabling the vector to replicate in the host cell in question.
The vector may also comprise a selectable marker, e.g. a gene the product of which complements a defect in the host cell, or a gene encoding resistance to e.g. antibiotics like ampicillin, kanamycin, chloramphenicol, erythromycin, tetracycline, spectinomycine, neomycin, hygromycin, methotrexate, or resistance to heavy metals, virus or herbicides, or which provides for prototrophy or auxotrophs. Examples of bacterial selectable markers are the dal genes from Bacillus subtilis or Bacillus licheniformis, resistance. A frequently used mammalian marker is the dihydrofolate reductase gene (DHFR). Suitable markers for yeast host cells are ADE2, HIS3, LEU2, LYS2, MET3, TRP1, and URA3. A selectable marker for use in a filamentous fungal host cell may be selected from the group including, but not limited to, amdS (acetamidase), argB (ornithine carbamoyltransferase), bar (phosphinothricin acetyltransferase), hygB (hygromycin phosphotransferase), niaD (nitrate reductase), pyrG (orotidine-5′-phosphate decarboxylase), sC (sulfate adenyltransferase), trpC (anthranilate synthase), and glufosinate resistance markers, as well as equivalents from other species. Particularly, for use in an Aspergillus cell are the amdS and pyrG markers of Aspergillus nidulans or Aspergillus oryzae and the bar marker of Streptomyces hygroscopicus. Furthermore, selection may be accomplished by co-transformation, e.g., as described in WO 91/17243, where the selectable marker is on a separate vector.
To direct a subtilase variant of the present invention into the secretory pathway of the host cells, a secretory signal sequence (also known as a leader sequence, prepro sequence or pre sequence) may be provided in the recombinant vector. The secretory signal sequence is joined to the DNA sequence encoding the enzyme in the correct reading frame. Secretory signal sequences are commonly positioned 5′ to the DNA sequence encoding the enzyme. The secretory signal sequence may be that normally associated with the enzyme or may be from a gene encoding another secreted protein.
The procedures used to ligate the DNA sequences coding for the present enzyme, the promoter and optionally the terminator and/or secretory signal sequence, respectively, or to assemble these sequences by suitable PCR amplification schemes, and to insert them into suitable vectors containing the information necessary for replication or integration, are well known to persons skilled in the art (cf., for instance, Sambrook et al.).
More than one copy of a nucleic acid sequence encoding an enzyme of the present invention may be inserted into the host cell to amplify expression of the nucleic acid sequence. Stable amplification of the nucleic acid sequence can be obtained by integrating at least one additional copy of the sequence into the host cell genome using methods well known in the art and selecting for transformants.
The nucleic acid constructs of the present invention may also comprise one or more nucleic acid sequences which encode one or more factors that are advantageous in the expression of the polypeptide, e.g., an activator (e.g., a trans-acting factor), a chaperone, and a processing protease. Any factor that is functional in the host cell of choice may be used in the present invention. The nucleic acids encoding one or more of these factors are not necessarily in tandem with the nucleic acid sequence encoding the polypeptide.
Host Cells
The DNA sequence encoding a subtilase variant of the present invention may be either homologous or heterologous to the host cell into which it is introduced. If homologous to the host cell, i.e. produced by the host cell in nature, it will typically be operably connected to another promoter sequence or, if applicable, another secretory signal sequence and/or terminator sequence than in its natural environment. The term “homologous” is intended to include a DNA sequence encoding an enzyme native to the host organism in question. The term “heterologous” is intended to include a DNA sequence not expressed by the host cell in nature. Thus, the DNA sequence may be from another organism, or it may be a synthetic sequence.
The host cell into which the DNA construct or the recombinant vector of the invention is introduced may be any cell that is capable of producing the present subtilase variants, such as prokaryotes, e.g. bacteria or eukaryotes, such as fungal cells, e.g. yeasts or filamentous fungi, insect cells, plant cells or mammalian cells.
Examples of bacterial host cells which, on cultivation, are capable of producing the subtilase variants of the invention are gram-positive bacteria such as strains of Bacillus, e.g. strains of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. coagulans, B. circulans, B. lautus, B. megaterium or B. thuringiensis, or strains of Streptomyces, such as S. lividans or S. murinus, or gram-negative bacteria such as Escherichia coli or Pseudomonas sp.
The transformation of the bacteria may be effected by protoplast transformation, electroporation, conjugation, or by using competent cells in a manner known per se (cf. Sambrook et al., supra).
When expressing the subtilase variant in bacteria such as E. coli, the enzyme may be retained in the cytoplasm, typically as insoluble granules (known as inclusion bodies), or it may be directed to the periplasmic space by a bacterial secretion sequence. In the former case, the cells are lysed and the granules are recovered and denatured after which the enzyme is refolded by diluting the denaturing agent. In the latter case, the enzyme may be recovered from the periplasmic space by disrupting the cells, e.g. by sonication or osmotic shock, to release the contents of the periplasmic space and recovering the enzyme.
When expressing the subtilase variant in gram-positive bacteria such as Bacillus or Streptomyces strains, the enzyme may be retained in the cytoplasm, or it may be directed to the extracellular medium by a bacterial secretion sequence. In the latter case, the enzyme may be recovered from the medium as described below.
Examples of host yeast cells include cells of a species of Candida, Kluyveromyces, Saccharomyces, Schizosaccharomyces, Pichia, Hansehula, or Yarrowia. In a particular embodiment, the yeast host cell is a Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomyces douglasii, Saccharomyces kluyveri, Saccharomyces norbensis or Saccharomyces oviformis cell. Other useful yeast host cells are a Kluyveromyces lactis, Kluyveromyces fragilis, Hansehula polymorpha, Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Ustilgo maylis, Candida maltose, Pichia guillermondii and Pichia methanolio cell (cf. Gleeson et al., J. Gen. Microbiol. 132, 1986, pp. 3459-3465; U.S. Pat. No. 4,882,279 and U.S. Pat. No. 4,879,231). Since the classification of yeast may change in the future, for the purposes of this invention, yeast shall be defined as described in Biology and Activities of Yeast (Skinner, F. A., Passmore, S. M., and Davenport, R. R., eds, Soc. App. Bacteriol. Symposium Series No. 9, 1980. The biology of yeast and manipulation of yeast genetics are well known in the art (see, e.g., Biochemistry and Genetics of Yeast, Bacil, M., Horecker, B. J., and Stopani, A. O. M., editors, 2nd edition, 1987; The Yeasts, Rose, A. H., and Harrison, J. S., editors, 2nd edition, 1987; and The Molecular Biology of the Yeast Saccharomyces, Strathern et al., editors, 1981). Yeast may be transformed using the procedures described by Becker and Guarente, In Abelson, J. N. and Simon, M. I., editors, Guide to Yeast Genetics and Molecular Biology, Methods in Enzymology, Volume 194, pp 182-187, Academic Press, Inc., New York; Ito et al., 1983, Journal of Bacteriology 153:163; and Hinnen et al., 1978, Proceedings of the National Academy of Sciences USA 75:1920.
Examples of filamentous fungal cells include filamentous forms of the subdivision Eumycota and Oomycota (as defined by Hawksworth et al., 1995, supra), in particular it may of the a cell of a species of Acremonium, such as A. chrysogenum, Aspergillus, such as A. awamori, A. foetidus, A. japonicus, A. niger, A. nidulans or A. oryzae, Fusarium, such as F. bactridioides, F. cerealis, F. crookwellense, F. culmorum, F. graminearum, F. graminum, F. heterosporum, F. negundi, F. reticulatum, F. roseum, F. sambucinum, F. sarcochroum, F. sulphureum, F. trichothecioides or F. oxysporum, Humicola, such as H. insolens or H. lanuginose, Mucor, such as M. miehei, Myceliophthora, such as M. thermophilum, Neurospora, such as N. crassa, Penicillium, such as P. purpurogenum, Thielavia, such as T. terrestris, Tolypocladium, or Trichoderma, such as T. harzianum, T. koningii, T. longibrachiatum, T. reesei or T. viride, or a teleomorph or synonym thereof. The use of Aspergillus spp. for the expression of proteins is described in, e.g., EP 272 277, EP 230 023.
Examples of insect cells include a Lepidoptera cell line, such as Spodoptera frugiperda cells or Trichoplusia ni cells (cf. U.S. Pat. No. 5,077,214). Culture conditions may suitably be as described in WO 89/01029 or WO 89/01028. Transformation of insect cells and production of heterologous polypeptides therein may be performed as described in U.S. Pat. No. 4,745,051; U.S. Pat. No. 4,775,624; U.S. Pat. No. 4,879,236; U.S. Pat. No. 5,155,037; U.S. Pat. No. 5,162,222; EP 397,485).
Examples of mammalian cells include Chinese hamster ovary (CHO) cells, HeLa cells, baby hamster kidney (BHK) cells, COS cells, or any number of other immortalized cell lines available, e.g., from the American Type Culture Collection. Methods of transfecting mammalian cells and expressing DNA sequences introduced in the cells are described in e.g. Kaufman and Sharp, J. Mol. Biol. 159 (1982), 601-621; Southern and Berg, J. Mol. Appl. Genet. 1 (1982), 327-341; Loyter et al., Proc. Natl. Acad. Sci. USA 79 (1982), 422-426; Wigler et al., Cell 14 (1978), 725; Corsaro and Pearson, Somatic Cell Genetics 7 (1981), 603, Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Inc., N.Y., 1987, Hawley-Nelson et al., Focus 15 (1993), 73; Ciccarone et al., Focus 15 (1993), 80; Graham and van der Eb, Virology 52 (1973), 456; and Neumann et al., EMBO J. 1 (1982), 841-845. Mammalian cells may be transfected by direct uptake using the calcium phosphate precipitation method of Graham and Van der Eb (1978, Virology 52:546).
Methods for Expression and Isolation of Proteins
To express an enzyme of the present invention the above mentioned host cells transformed or transfected with a vector comprising a nucleic acid sequence encoding an enzyme of the present invention are typically cultured in a suitable nutrient medium under conditions permitting the production of the desired molecules, after which these are recovered from the cells, or the culture broth.
The medium used to culture the host cells may be any conventional medium suitable for growing the host cells, such as minimal or complex media containing appropriate supplements. Suitable media are available from commercial suppliers or may be prepared according to published recipes (e.g. in catalogues of the American Type Culture Collection). The media may be prepared using procedures known in the art (see, e.g., references for bacteria and yeast; Bennett, J. W. and LaSure, L., editors, More Gene Manipulations in Fungi, Academic Press, C A, 1991).
If the enzymes of the present invention are secreted into the nutrient medium, they may be recovered directly from the medium. If they are not secreted, they may be recovered from cell lysates. The enzymes of the present invention may be recovered from the culture medium by conventional procedures including separating the host cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt, e.g. ammonium sulphate, purification by a variety of chromatographic procedures, e.g. ion exchange chromatography, gelfiltration chromatography, affinity chromatography, or the like, dependent on the enzyme in question.
The enzymes of the invention may be detected using methods known in the art that are specific for these proteins. These detection methods include use of specific antibodies, formation of a product, or disappearance of a substrate. For example, an enzyme assay may be used to determine the activity of the molecule. Procedures for determining various kinds of activity are known in the art.
The enzymes of the present invention may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing (IEF), differential solubility (e.g., ammonium sulfate precipitation), or extraction (see, e.g., Protein Purification, J-C Janson and Lars Ryden, editors, VCH Publishers, New York, 1989).
When an expression vector comprising a DNA sequence encoding an enzyme of the present invention is transformed/transfected into a heterologous host cell it is possible to enable heterologous recombinant production of the enzyme. An advantage of using a heterologous host cell is that it is possible to make a highly purified enzyme composition, characterized in being free from homologous impurities, which are often present when a protein or peptide is expressed in a homologous host cell. In this context homologous impurities mean any impurity (e.g. other polypeptides than the enzyme of the invention) which originates from the homologous cell where the enzyme of the invention is originally obtained from.
DETERGENT APPLICATIONS
The enzyme of the invention may be added to and thus become a component of a detergent composition.
The detergent composition of the invention may for example be formulated as a hand or machine laundry detergent composition including a laundry additive composition suitable for pre-treatment of stained fabrics and a rinse added fabric softener composition, or be formulated as a detergent composition for use in general household hard surface cleaning operations, or be formulated for hand or machine dishwashing operations.
In a specific aspect, the invention provides a detergent additive comprising the enzyme of the invention. The detergent additive as well as the detergent composition may comprise one or more other enzymes such as a protease, a lipase, a cutinase, an amylase, a carbohydrase, a cellulase, a pectinase, a mannanase, an arabinase, a galactanase, a xylanase, an oxidase, e.g., a laccase, and/or a peroxidase.
In general the properties of the chosen enzyme(s) should be compatible with the selected detergent, (i.e. pH-optimum, compatibility with other enzymatic and non-enzymatic ingredients, etc.), and the enzyme(s) should be present in effective amounts.
Proteases:
Suitable proteases include those of animal, vegetable or microbial origin. Microbial origin is preferred. Chemically modified or protein engineered mutants are included. The protease may be a serine protease or a metallo protease, preferably an alkaline microbial protease or a trypsin-like protease. Examples of alkaline proteases are subtilisins, especially those derived from Bacillus, e.g., subtilisin Novo, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168 (described in WO 89/06279). Examples of trypsin-like proteases are trypsin (e.g. of porcine or bovine origin) and the Fusarium protease described in WO 89/06270 and WO 94/25583.
Examples of useful proteases are the variants described in WO 92/19729, WO 98/20115, WO 98/20116, and WO 98/34946, especially the variants with substitutions in one or more of the following positions: 27, 36, 57, 76, 87, 97, 101, 104, 120, 123, 167, 170, 194, 206, 218, 222, 224, 235 and 274.
Preferred commercially available protease enzymes include Alcalase®, Savinase®, Primase®, Duralase®, Esperase®, Ovozyme® and Kannase® (Novozymes A/S), MaxataseTM MaxacalTM, MaxapemTM, ProperaseTM, PurafectTM, Purafect OxPTM, FN2TM FN3TM and FN4TM (Genencor International Inc.).
Lipases:
Suitable lipases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful lipases include lipases from Humicola (synonym Thermomyces), e.g. from H. lanuginosa (T. lanuginosus) as described in EP 258 068 and EP 305 216 or from H. insolens as described in WO 96/13580, a Pseudomonas lipase, e.g. from P. alcaligenes or P. pseudoalcaligenes (EP 218 272), P. cepacia (EP 331 376), P. stutzeri (GB 1,372,034), P. fluorescens, Pseudomonas sp. strain SD 705 (WO 95/06720 and WO 96/27002), P. wisconsinensis (WO 96/12012), a Bacillus lipase, e.g. from B. subtilis (Dartois et al. (1993), Biochemica et Biophysica Acta, 1131, 253-360), B. stearothermophilus (JP 64/744992) or B. pumilus (WO 91/16422).
Other examples are lipase variants such as those described in WO 92/05249, WO 94/01541, EP 407 225, EP 260 105, WO 95/35381, WO 96/00292, WO 95/30744, WO 94/25578, WO 95/14783, WO 95/22615, WO 97/04079 and WO 97/07202.
Preferred commercially available lipase enzymes include Lipex®, Lipolase® and Lipolase Ultra® (Novozymes A/S).
Amylases:
Suitable amylases (α and/or β) include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, α-amylases obtained from Bacillus, e.g. a special strain of B. licheniformis, described in more detail in GB 1,296,839.
Examples of useful amylases are the variants described in WO 94/02597, WO 94/18314, WO 96/23873, and WO 97/43424, especially the variants with substitutions in one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 181, 188, 190, 197, 202, 208, 209, 243, 264, 304, 305, 391, 408, and 444.
Commercially available amylases are DuramylTM, TermamylTM, FungamylTM and BANTM (Novozymes A/S), RapidaseTM and PurastarTM (from Genencor International Inc.).
Cellulases:
Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, e.g. the fungal cellulases produced from Humicola insolens, Myceliophthora thermophila and Fusarium oxysporum disclosed in U.S. Pat. No. 4,435,307, U.S. Pat. No. 5,648,263, U.S. Pat. No. 5,691,178, U.S. Pat. No. 5,776,757 and WO 89/09259.
Especially suitable cellulases are the alkaline or neutral cellulases having color care benefits. Examples of such cellulases are cellulases described in EP 0 495 257, EP 0 531 372, WO 96/11262, WO 96/29397, WO 98/08940. Other examples are cellulase variants such as those described in WO 94/07998, EP 0 531 315, U.S. Pat. No. 5,457,046, U.S. Pat. No. 5,686,593, U.S. Pat. No. 5,763,254, WO 95/24471, WO 98/12307 and PCT/DK98/00299.
Commercially available cellulases include Celluzyme® and Carezyme® (Novozymes A/S), ClazinaseTM, and Puradax HATM (Genencor International Inc.), and KAC-500(B)TM (Kao Corporation).
Peroxidases/Oxidases:
Suitable peroxidases/oxidases include those of plant, bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful peroxidases include peroxidases from Coprinus, e.g. from C. cinereus, and variants thereof as those described in WO 93/24618, WO 95/10602, and WO 98/15257.
Commercially available peroxidases include Guardzyme® (Novozymes A/S).
The detergent enzyme(s) may be included in a detergent composition by adding separate additives containing one or more enzymes, or by adding a combined additive comprising all of these enzymes. A detergent additive of the invention, i.e. a separate additive or a combined additive, can be formulated e.g. as a granulate, a liquid, a slurry, etc. Preferred detergent additive formulations are granulates, in particular non-dusting granulates, liquids, in particular stabilized liquids, or slurries.
Non-dusting granulates may be produced, e.g., as disclosed in U.S. Pat. Nos. 4,106,991 and 4,661,452 and may optionally be coated by methods known in the art. Examples of waxy coating materials are poly(ethylene oxide) products (polyethyleneglycol, PEG) with mean molar weights of 1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains from 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono- and di- and triglycerides of fatty acids. Examples of film-forming coating materials suitable for application by fluid bed techniques are given in GB 1483591. Liquid enzyme preparations may, for instance, be stabilized by adding a polyol such as propylene glycol, a sugar or sugar alcohol, lactic acid or boric acid according to established methods. Protected enzymes may be prepared according to the method disclosed in EP 238,216.
The detergent composition of the invention may be in any convenient form, e.g., a bar, a tablet, a powder, a granule, a paste or a liquid. A liquid detergent may be aqueous, typically containing up to 70% water and 0-30% organic solvent, or non-aqueous.
The detergent composition comprises one or more surfactants, which may be non-ionic including semi-polar and/or anionic and/or cationic and/or zwitterionic. The surfactants are typically present at a level of from 0.1% to 60% by weight.
When included therein the detergent will usually contain from about 1% to about 40% of an anionic surfactant such as linear alkylbenzenesulfonate, alpha-olefinsulfonate, alkyl sulfate (fatty alcohol sulfate), alcohol ethoxysulfate, secondary alkanesulfonate, alpha-sulfo fatty acid methyl ester, alkyl- or alkenylsuccinic acid or soap.
When included therein the detergent will usually contain from about 0.2% to about 40% of a non-ionic surfactant such as alcohol ethoxylate, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamineoxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, polyhydroxy alkyl fatty acid amide, or N-acyl N-alkyl derivatives of glucosamine (“glucamides”).
The detergent may contain 0-65% of a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, carbonate, citrate, nitrilotriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (e.g. SKS-6 from Hoechst).
The detergent may comprise one or more polymers. Examples are carboxymethylcellulose, poly(vinylpyrrolidone), poly (ethylene glycol), poly(vinyl alcohol), poly(vinylpyridine-N-oxide), poly(vinylimidazole), polycarboxylates such as polyacrylates, maleic/acrylic acid copolymers and lauryl methacrylate/acrylic acid copolymers.
The detergent may contain a bleaching system which may comprise a H2O2 source such as perborate or percarbonate which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine or nonanoyloxybenzenesulfonate. Alternatively, the bleaching system may comprise peroxyacids of e.g. the amide, imide, or sulfone type.
The enzyme(s) of the detergent composition of the invention may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition may be formulated as described in e.g. WO 92/19709 and WO 92/19708.
The detergent may also contain other conventional detergent ingredients such as e.g. fabric conditioners including clays, foam boosters, suds suppressors, anti-corrosion agents, soil-suspending agents, anti-soil redeposition agents, dyes, bactericides, optical brighteners, hydrotropes, tarnish inhibitors, or perfumes.
It is at present contemplated that in the detergent compositions any enzyme, in particular the enzyme of the invention, may be added in an amount corresponding to 0.01-200 mg of enzyme protein per liter of wash liquor, preferably 0.05-50 mg of enzyme protein per liter of wash liquor, in particular 0.1-10 mg of enzyme protein per liter of wash liquor.
The enzyme of the invention may additionally be incorporated in the detergent formulations disclosed in WO 97/07202 which is hereby incorporated as reference.
MATERIALS AND METHODS
Textiles
Standard textile pieces are obtained from EMPA St. Gallen, Lerchfeldstrasse 5, CH-9014 St. Gallen, Switzerland. Especially type EMPA 116 (cotton textile stained with blood, milk and ink) and EMPA 117 (polyester/cotton textile stained with blood, milk and ink).
Method for Producing a Subtilase Variant
The present invention provides a method of producing an isolated enzyme according to the invention, wherein a suitable host cell, which has been transformed with a DNA sequence encoding the enzyme, is cultured under conditions permitting the production of the enzyme, and the resulting enzyme is recovered from the culture.
When an expression vector comprising a DNA sequence encoding the enzyme is transformed into a heterologous host cell it is possible to enable heterologous recombinant production of the enzyme of the invention. Thereby it is possible to make a highly purified subtilase composition, characterized in being free from homologous impurities.
The medium used to culture the transformed host cells may be any conventional medium suitable for growing the host cells in question. The expressed subtilase may conveniently be secreted into the culture medium and may be recovered there-from by well-known procedures including separating the cells from the medium by centrifugation or filtration, precipitating proteinaceous components of the medium by means of a salt such as ammonium sulfate, followed by chromatographic procedures such as ion exchange chromatography, affinity chromatography, or the like.
Example 1
Construction of Library of Savinase Variants
A library, based on Savinase positions V28, I35, T71, I72, A73, M175 and T224 (BPN′ numbering) was synthesized. The library contains exclusively TY145-suggested alterations and covers the introduced mutations V28I,A,L; I35V,A,L; T71S; I72A,G,V; A73L,G; M175V,A; T224S,A introduced in oligopeptides, some of which were doped. Doping of nucleotide bases from a desired doping of individual amino acid residues, which is used for the example below, can be calculated as described above.
In the attached sequence listing, the doped nucleotides below have been given the nucleotide symbols recommended by the WIPO Standard ST25.
The constructed oligopeptide primers are listed below. The primers are named after which positions are subject to modifications, thus 28-35-CN has alterations in positions 28 and 35, 71-72-73-NC has alterations in positions 71, 72 and 73, and so forth.







28-35-CN, 


SEQ ID NO: 7


5′-TAG ATC TGG ATG AGT GGA (50%T/50%A)


 


(80%A/10%G/10%C)(75%T/25%C) CCC TGT ATC GAG GAC 


 


AGC (75%A/25%T)(90%A/10%G)(80%C/10%T/10%G) TTT 


 


TAC ACC AGA ACC TGT-3′


 


28-35-NC, 


SEQ ID NO: 8


5′-TCC ACT CAT CCA GAT CTA-3′


 


(I) 71-72-73-CN, 


SEQ ID NO: 9


5′-AAT CGA ATT GTT TAA AGC AGC (65%-1/35%A)


 


(80%A/10%C/10%G)(75%T/25%C)(90%C/10%T)G


 


(90%T/10%A) CCC GGC CAC ATG CGT GCC-3′


 


(II) 71-72-73-CN, 


SEQ ID NO: 10


5′-AAT CGA ATT GTT TAA AGC AAG (65%T/35%A)


 


(80%A/10%C/10%G)(75%T/25%C)(90%C/10%T)G


 


(90%T/10%A) CCC GGC CAC ATG CGT GCC-3′


 


(III) 71-72-73-CN, 


SEQ ID NO: 11


5′-AAT CGA ATT GTT TAA AGC GCC (65%T/35%A)


 


(80%A/10%C/10%G)(75%T/25%C)(90%C/10%T)G


 


(90%T/10%A) CCC GGC CAC ATG CGT GCC-3′


 


71-72-73-NC, 


SEQ ID NO: 12


5′ GCT TTA AAC AAT TCG ATT 3′


 


139, 


SEQ ID NO: 13


5′-GAT TAA CGC GTT GCC GCT TCT GCG-3′


 


(I) 175-CN (90%), 


SEQ ID NO: 14


5′-ATC AGT AGC TCC GAC TGC CA(90%T/10%C) TGC GTT


 


CGC ATA GCG CGC-3′


 


(II) 175-CN (10%), 


SEQ ID NO: 15


5′-ATC AGT AGC TCC GAC TGC CGC TGC GTT CGC ATA 


 


GCG CGC-3′


 


175-NC, 


SEQ ID NO: 16


5′-GCA GTC GGA GCT ACT GAT-3′


 


224-CN, 


SEQ ID NO: 17


5′-CGC ACC TGC AAC ATG AGG CG(80%T/10%C/10%A) AGC


 


CAT CGA TGT ACC GTT-3′


 


224-NC, 


SEQ ID NO: 18


5′-CCT CAT GTT GCA GGT GCG-3′


 


317, 


SEQ ID NO: 19


5′-TGG CGC AAT CGG TAC CAT GGG G-3′






The Savinase gene was used as template for five individual PCR reactions under standard PCR conditions (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor, 1989) where the oligos were combined as follows:
317 with 28-35-CN, 28-35-NC with 71-72-73-CN (a mixture of 80% (I) 71-72-73-CN, 10% (II) 71-72-73-CN and 10% (III) 71-72-73-CN), 71-72-73-NC with 175-CN (a mixture of 90% (I) 175-CN and 10% (II) 175-CN), 175-NC with 224-CN, 224-NC with 139, giving PCR products of 125 bp, 126 bp, 312 bp, 165 by and 158 by respectively.
The library was assembled by an additional PCR reaction where the five PCR products were mixed in equal molar amounts. Thereby the library contained a large number of different Savinase variants altered in one or more of the mentioned positions. The PCR reaction was assembled using a PTC-200 DNA Engine (MJ Research, Watertown, Mass.) and the following cycling parameters: 1 cycle of 2 min at 94° C. followed by 25 cycles of 30 sec at 94° C., 30 sec at 55° C. and 1 min at 68° C., and 1 cycle of 2 min at 68° C. The library was cloned by PCR multimerization (Shafikhani et al. 1997) into Savinase expression vector psx222 and transformed into a B. subtilis host for expression. Subsequently Savinase variants were isolated from the library, purified and characterized.
Likewise, properties from a BPN′ like subtilase could be transferred to TY145 like subtilase by applying a similar procedure.
Example 2
Transfer of Regions from TY145 to BPN′ Subtilases
The below mentioned highly mobile regions in TY145 were selected for transfer from TY145 to Savinase. The Savinase regions (BPN′ numbering) were deleted and the TY145 regions (SEQ ID NO: 1) inserted instead. In addition regions can be selected for transfer between the psycrophiles TA41/TA39 and BPN′ type protease like Savinase, or from TA39/TA41 to TY145 type non-psychrophilic subtilases.









SEGMENT I



TY145 



(SEQ ID NO: 22)



SAKDSLIASAVD, positions 144-155 



 



Savinase 



(SEQ ID NO: 23)



PSPSATLEQAVN, positions 129-140 



 



SEGMENT II



TY145 



(SEQ ID NO: 24)



AGNSGSGSNTIGFPGGLV, positions 168-185



 



Savinase 



(SEQ ID NO: 25)



SGNSGAGSISYPARYA, positions 153-172 



 



SEGMENT IV



TY145 



(SEQ ID NO: 26)



ASVESTWYTGGYNTIS, positions 233-248 



 



Savinase 



(SEQ ID NO: 27)



VNVQSTYPGSTYASLN, positions 203-218 






Savinase variants modified by receiving respectively segments II (Hybrid II), IV (Hybrid IV) or I+II (Hybrid I+II) from TY145 were observed to exhibit subtilase activity as determined by the formation of clearing zones on skim milk powder plates.
Example 3
Transfer of Regions from S39 and S41 to BPN′ Subtilases
The below mentioned highly mobile regions in the TA39 subtilase S39 and the TA41 subtilase S41, determined by the previous described homology building programs, were selected for transfer to Savinase. The Savinase regions (BPN′ numbering) were deleted and the S39 regions or S41 regions were inserted instead. Below, the S39 and S41 regions are numbered according to FIG. 1. In addition regions can be selected for transfer between the psychrophiles TA41/TA39 and TY145 type non-psychrophilic subtilases. Savinase variant V104S was used as acceptor for the S39 segment II.









SEGMENT I



S39 



(SEQ ID NO: 28)



MSLGSSG, positions 137-143 



 



Savinase2 



(SEQ ID NO: 29)



LSLGSPS, positions 124-130 



 



SEGMENT II



S39 



(SEQ ID NO: 30)



MSLGSSGESSLI, positions 137-148 



 



Savinase variant V104S 



(SEQ ID NO: 31)



LSLGSPSPSATL, positions 124-135 



 



SEGMENT III



S39



(SEQ ID NO: 32)



NNSSITQT, positions 15-22 



 



Savinase 



(SEQ ID NO: 33)



VQAPAAHN, positions 11-18 



 



SEGMENT IV



S39 



(SEQ ID NO: 34)



TVGTTYTN, positions 55-62 



 



Savinase2 



(SEQ ID NO: 35)



VPG*EPST, positions 51*-58 



 



SEGMENT V



S39 



RQ, positions 68-69



 



Savinase 



GN, positions 61-62



 



SEGMENT VI



S39 



(SEQ ID NO: 36)



SGESSLI, positions 142-148 



 



Savinase 



(SEQ ID NO: 37)



PSPSATL, positions 129-135 



 



SEGMENT VII



S39 



(SEQ ID NO: 38)



WFDGGYATI, positions 237-245 



 



Savinase 



(SEQ ID NO: 39)



YPGSTYASL, positions 209-217 






Savinase variants modified by receiving respectively segments I or II from S39 were observed to have subtilase activity against the substrate suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA). The subtilase activity was determined in a temperature profile assay where specific activities i.e. micromole substrate per minute per mg enzyme against before mentioned substrates, were determined at every 5 degrees Celsius. The measurements were done in a Tris-base buffer pH 9.
To measure subtilase activity in suc-AAPF-pNA: 100 uL 1.56 mM Suc-Ala-Ala-Pro-Phe-pNA in 0.1 M Tris was added to 100 uL Tris-base, pH 9.0 buffer and 20 uL enzyme. The development of the degradation product pNA (paranitrophenol) was measured as initial velocities at 405 nm on an Elisa Reader for 1 minute.
The Savinase variant with segment I substituted had less specific activity against suc-AAPF-pNA compared to Savinase, whereas the Savinase variant with segment II substituted had more than 2 times higher specific activity against suc-AAPF-pNA than Savinase. In an AMSA-test (performed like described in Example 5 herein) the wash performance was shown to be preserved in Savinase variant with segment II compared to Savinase.
Further, four Savinase variants were constructed with the following combinations of segments from S39:
Segments III, V and VII; Segments III and V; Segments III, V, VI and Segments III and IV. All four Savinase variants showed subtilase activity on skim milk plates.
Segments from the S41 subtilase suggested for transfer to Savinase are:









SEGMENT VIII



S41 



(SEQ ID NO: 40)



TVGTNFTD, positions 55-62 



 



Savinase 



(SEQ ID NO: 41)



VPG*EPST, positions 51-58 



 



SEGMENT IX



S41 



(SEQ ID NO: 42)



NGGTGS, positions 82-87 



 



Savinase 



(SEQ ID NO: 43)



ALNNSI, positions 74-79 



 



SEGMENT X



S41 



(SEQ ID NO: 44)



DDGSGYA, positions 106-112 



 



Savinase 



(SEQ ID NO: 45)



ASGSGSV, positions 98-104 



 



SEGMENT XI



S41 



(SEQ ID NO: 46)



WAQSPAA, positions 263-269 



 



Savinase 



(SEQ ID NO: 47)



KQKNPSW, positions 235-241






Four Savinase variants were constructed with the following segments from S41: Segment X; Segments IX and X; Segments VIII and X; and Segments X and XI. All four Savinase variants showed subtilase activity on skim milk plates.
AMSA wash tests were performed on variants with Segment X and Segments X and XI like described in Example 5 herein.
The assay was conducted under the experimental conditions specified below:













Detergent base
Omo Acao


Detergent dosage
2.5 g/l


Test solution volume
160 micro l


pH
10-10.5 adjusted with NaHCO3


Wash time
14 minutes


Temperature
15° C.


Water hardness
9° dH


Enzyme concentration in test solution
5 nM, 10 nM and 30 nM


Test material
EMPA 117









The wash performance score (described in Example 5 herein) of the Savinase variants with Segment X and Segments X and XI was S (1) indicating an improved wash performance compared to Savinase.
Example 4
Purification and Assessment of Enzyme Concentration
After fermentation, purification of subtilisin variants was accomplished using Hydrophobic Charge Induction Chromatography (HCIC) and subsequent vacuum filtration.
To capture the enzyme, the HCIC uses a cellulose matrix to which 4-Mercapto-Ethyl-Pyridine (4-MEP) is bound.
Beads of the cellulose matrix sized 80-100 μm were mixed with a media containing yeast and the transformed B. subtilis capable of secreting the subtilisin variants and incubated at pH 9.5 in Unifilter® microplates.
As 4-MEP is hydrophobic at pH>7 and the subtilisin variants are hydrophobic at pH 9.5 a hydrophobic association was made between the secreted enzyme and the 4-MEP on the beads. After incubation the media and cell debris were removed by vacuum filtration while the beads and enzyme were kept on the filter.
To elute the enzyme from the beads the pH was lowered by washing the filter with an elution buffer (pH 5) resulting in the enzyme parting from the beads. The enzyme was then retrieved from the buffer.
The concentration of the purified subtilisin enzyme variants was assessed by active site titration (AST).
The purified enzyme was incubated with the high affinity inhibitor CI-2A at different concentrations to inhibit a varying amount of the active sites. The protease and inhibitor binds to each other at a 1:1 ratio and accordingly the enzyme concentration can be directly related to the concentration of inhibitor, at which all protease is inactive. To measure the residual protease activity, a substrate suc-AAPF-pNA (0.6 mM Suc-Ala-Ala-Pro-Phe-pNA in Tris/HCl buffer) was added after the incubation with inhibitor and during the following 4 minutes the development of the degradation product pNA (paranitrophenol) was measured periodically at 405 nm on an Elisa Reader.
Example 5
Wash Performance of Detergent Compositions Comprising Modified Enzymes
Wash performance of detergent compositions comprising enzyme hybrids or enzyme variants of the present invention was tested at low washing temperature.
The Savinase variant Hybrid IV of Example 2 was tested for washing performance in two different assays; a microliter scale assay (AMSA) and a milliliter scale assay (Mini wash).
AMSA
The enzyme variants of the present application were tested using the Automatic Mechanical Stress Assay (AMSA). With the AMSA test the wash performance of a large quantity of small volume enzyme-detergent solutions can be examined. The AMSA plate has a number of slots for test solutions and a lid firmly squeezing the textile swatch to be washed against all the slot openings. During the washing time, the plate, test solutions, textile and lid were vigorously shaken to bring the test solution in contact with the textile and apply mechanical stress. For further description see WO 02/42740 especially the paragraph “Special method embodiments” at page 23-24.
The assay was conducted under the experimental conditions specified below:













Detergent base
Omo Acao


Detergent dosage
1.5 g/l


Test solution volume
160 micro l


pH
10-10.5 adjusted with NaHCO3


Wash time
12 minutes


Temperature
20° C.


Water hardness
9° dH


Enzyme concentration in test solution
5 nM, 10 nM and 30 nM


Test material
EMPA 117









After washing the textile pieces were flushed in tap water and air-dried.
The performance of the enzyme variant was measured as the brightness of the color of the textile samples washed with that specific enzyme variant. Brightness can also be expressed as the intensity of the light reflected from the textile sample when luminated with white light. When the textile is stained the intensity of the reflected light is lower, than that of a clean textile. Therefore the intensity of the reflected light can be used to measure wash performance of an enzyme variant.
Color measurements were made with a professional flatbed scanner (PFU DL2400pro), which was used to capture an image of the washed textile samples. The scans were made with a resolution of 200 dpi and with an output color dept of 24 bits. In order to get accurate results, the scanner was frequently calibrated with a Kodak reflective 1T8 target.
To extract a value for the light intensity from the scanned images, a special designed software application was used (Novozymes Color Vector Analyzer). The program retrieves the 24 bit pixel values from the image and converts them into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector:

Int=√{square root over (r2+g2+b2)}.

The wash performance (P) of the variants was calculated in accordance with the below formula:

P=Int(v)−Int(r)

where

Int(v) is the light intensity value of textile surface washed with enzyme variant and
Int(r) is the light intensity value of textile surface washed with the reference enzyme subtilisin 309 (BLSAVI).
The result of the AMSA wash of Hybrid IV was a Performance Score of S (2) in accordance with the definition:
Performance Scores (S) are summing up the performances (P) of the tested enzyme variants as:
S (2) which indicates that the variant performs better than the reference at all three concentrations (5, 10 and 30 nM) and
S (1) which indicates that the variant performs better than the reference at one or two concentrations.
Mini Wash Assay
The milliliter scale wash performance assay was conducted under the following conditions:













Detergent base
Omo Acao detergent powder


Detergent dose
1.5 g/l


pH
′′as is′′ in the current detergent solution and is not adjusted.


Wash time
14 min.


Temperature
20° C. 


Water hardness
9° dH, adjusted by adding CaCl2 * 2H2O; MgCl2 * 6H2O; 



NaHCO3 (Ca2+:Mg2+:HCO3− = 2:1:6) to milli-Q water.


Enzymes
Hybrid IV, Savinase


Enzyme conc.
5 nM, 10 nM


Test system
125 ml glass beakers. Textile dipped in test solution.



Continuously up and down, 50 times per minute


Textile/volume
1 textile piece (13 × 3 cm) in 50 ml test solution


Test material
EMPA 117 textile swatches









After wash the measurement of remission from the test material was done at 460 nm using a Zeiss MCS 521 VIS spectrophotometer. The measurements were done according to the manufacturer's protocol.
As shown in Table 1 the textile washed with the Savinase variant Hybrid IV at 20° C. in Omo Acao has a higher remission than the textile washed with the parent. This result indicates that this variant has better wash performance at low temperature than the parent Savinase.







TABLE 1







Wash performance results of the subtilase variant in Omo 


Acao for a dosage of 5 nM and10 nM enzyme.









Enzyme
Remission, 5 nM enzyme
Remission, 10 nM enzyme





Blank (no enzyme)
12.0
12.3


Savinase
15.8
17.4


Hybrid IV
17.0
18.3









As it can be concluded from Table 1 the modified subtilases of the invention exhibits an improvement in wash performance.
Example 6
Wash performance of detergent compositions comprising enzyme variants of the present invention was tested at low washing temperature using the Automatic Mechanical Stress Assay (AMSA) as described in Example 5 herein.







TABLE 2







AMSA wash results of subtilase variants.








Enzyme variant 
Performance score





V28I + *98aD + T224S
2


*98aS + P131F + M175V + T224A
1


















APPENDIX 1







REMARK
3
REFINEMENT.










REMARK
3
 PROGRAM
: REFMAC 5.0


REMARK
3
 AUTHORS
: MURSHUDOV, VAGIN, DODSON


REMARK
3









REMARK
3
  REFINEMENT TARGET : MAXIMUM LIKELIHOOD


REMARK
3


REMARK
3
 DATA USED IN REFINEMENT.











REMARK
3
 RESOLUTION RANGE HIGH
 (ANGSTROMS) :
 1.80


REMARK
3
 RESOLUTION RANGE LOW
(ANGSTROMS) :
56.80











REMARK
3
 DATA CUTOFF
 (SIGMA(F)) :
NONE











REMARK
3
 COMPLETENESS FOR RANGE
(%) :
99.88











REMARK
3
 NUMBER OF REFLECTIONS
:
38045


REMARK
3


REMARK
3
 FIT TO DATA USED IN REFINEMENT.










REMARK
3
 CROSS-VALIDATION METHOD
: THROUGHOUT


REMARK
3
 FREE R VALUE TEST SET SELECTION
 : RANDOM











REMARK
3
 R VALUE
(WORKING + TEST SET)
 : 0.15648


REMARK
3
 R VALUE
(WORKING SET)
:  0.15487










REMARK
3
 FREE R VALUE
: 0.18707











REMARK
3
 FREE R VALUE TEST SET SIZE
(%)
:   5.0










REMARK
3
 FREE R VALUE TEST SET COUNT
:  2009


REMARK
3









REMARK
3
 FIT IN THE HIGHEST RESOLUTION BIN.











REMARK
3
 TOTAL NUMBER OF BINS USED
 :
  20


REMARK
3
 BIN RESOLUTION RANGE HIGH
 :
  1.796


REMARK
3
 BIN RESOLUTION RANGE LOW
:
  1.842












REMARK
3
 REFLECTION IN BIN
(WORKING SET)
 :
  2738


REMARK
3
 BIN R VALUE
(WORKING SET)
:
  0.191











REMARK
3
 BIN FREE R VALUE SET COUNT
:
  138











REMARK
3
 BIN FREE R VALUE
:
0.234


REMARK
3









REMARK
3
 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.











REMARK
3
 ALL ATOMS
:
3156


REMARK
3


REMARK
3
 B VALUES.











REMARK
3
 FROM WILSON PLOT
(A**2) :
NULL


REMARK
3
 MEAN B VALUE
(OVERALL, A**2)  :
 14.804









REMARK
3
 OVERALL ANISOTROPIC B VALUE.











REMARK
3
  B11 (A**2)  :
0.28



REMARK
3
  B22 (A**2)  :
−0.86


REMARK
3
  B33 (A**2)  :
0.58


REMARK
3
  B12 (A**2)  :
0.00


REMARK
3
  B13 (A**2)  :
0.00


REMARK
3
  B23 (A**2)  :
0.00


REMARK
3











REMARK
3
 ESTIMATED OVERALL COORDINATE ERROR.




REMARK
3
 ESU BASED ON R VALUE
 (A):
0.100


REMARK
3
 ESU BASED ON FREE R VALUE
 (A):
 0.098


REMARK
3
 ESU BASED ON MAXIMUM LIKELIHOOD
  (A):
 0.093


REMARK
3
 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD
  (A**2):
  2.910


REMARK
3


REMARK
3
CORRELATION COEFFICIENTS.











REMARK
3
 CORRELATION COEFFICIENT FO-FC
:
0.963


REMARK
3
 CORRELATION COEFFICIENT FO-FC FREE
 :
 0.952


REMARK
3










REMARK
3
 RMS DEVIATIONS FROM IDEAL VALUES
COUNT  RMS  WEIGHT











REMARK
3
 BOND LENGTHS REFINED ATOMS
(A):  
 2798 ; 0.021 ; 0.021


REMARK
3
 BOND LENGTHS OTHERS
(A): 
2500 ; 0.001 ; 0.020


REMARK
3
 BOND ANGLES REFINED ATOMS
(DEGREES): 
 3805 ; 1.859 ; 1.943


REMARK
3
 BOND ANGLES OTHERS
(DEGREES):  
 5821 ; 0.854 ; 3.000


REMARK
3
 TORSION ANGLES, PERIOD 1
(DEGREES): 
  372 ; 5.125 ; 3.000


REMARK
3
 TORSION ANGLES, PERIOD 3
(DEGREES): 
  462 ; 16.877 ;15.000


REMARK
3
 CHIRAL-CENTER RESTRAINTS
(A**3):  
 437 ; 0.119 ; 0.200


REMARK
3
 GENERAL PLANES REFINED ATOMS
(A): 
  3201 ; 0.009 ; 0.020


REMARK
3
 GENERAL PLANES OTHERS
(A): 
 535 ; 0.004 ; 0.020











REMARK
3
 NON-BONDED CONTACTS REFINED ATOMS
(A):
   610 ; 0.228 ; 0.300


REMARK
3
 NON-BONDED CONTACTS OTHERS
(A): 
 2548 ; 0.203 ; 0.300


REMARK
3
 H-BOND (X . . . Y) REFINED ATOMS
(A): 
 374 ; 0.184 ; 0.500


REMARK
3
 H-BOND (X . . . Y) OTHERS
(A):
  3 ; 0.279 ; 0.500


REMARK
3
 POTENTIAL METAL-ION REFINED ATOMS
(A):
  16 ; 0.119 ; 0.500


REMARK
3
 SYMMETRY VDW REFINED ATOMS
(A): 
   7 ; 0.127 ; 0.300


REMARK
3
 SYMMETRY VDW OTHERS
(A):  
 27 ; 0.152 ; 0.300


REMARK
3
 SYMMETRY H-BOND REFINED ATOMS
(A): 
  37 ; 0.278 ; 0.500


REMARK
3










REMARK
3
 ISOTROPIC THERMAL FACTOR RESTRAINTS.
COUNT  RMS  WEIGHT











REMARK
3
 MAIN-CHAIN BOND REFINED ATOMS
(A**2):
1840 ; 1.131 ;  1.500


REMARK
3
 MAIN-CHAIN ANGLE REFINED ATOMS
 (A**2):
 2941 ;  1.781 ;  2.000


REMARK
3
 SIDE-CHAIN BOND REFINED ATOMS
(A**2):
 958 ; 2.873 ; 3.000


REMARK
3
 SIDE-CHAIN ANGLE REFINED ATOMS
 (A**2):
 864 ;  4.300 ;  4.500


REMARK
3









REMARK
3
 NCS RESTRAINTS STATISTICS


REMARK
3
 NUMBER OF NCS GROUPS  :  NULL


REMARK
3


REMARK
3


REMARK
3
 TLS DETAILS


REMARK
3
 NUMBER OF TLS GROUPS  :  NULL


REMARK
3


REMARK
3


REMARK
3
 BULK SOLVENT MODELLING.


REMARK
3
 METHOD USED : BABINET MODEL WITH MASK


REMARK
3
 PARAMETERS FOR MASK CALCULATION










REMARK
3
 VDW PROBE RADIUS
:  1.40


REMARK
3
 ION PROBE RADIUS
:  0.80


REMARK
3
 SHRINKAGE RADIUS
:  0.80


REMARK
3









REMARK
3
 OTHER REFINEMENT REMARKS:


REMARK
3
 HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS


REMARK
3















CISPEP
1
GLY
A
172
SER
A
173
0.00


CISPEP
2
PHE
A
180
PRO
A
181
0.00


SSBOND
1
CYS
A
 52
CYS
A
 66








CRYST1
58.753  66.838  107.082  90.00  90.00  90.00  P  21  21  21











SCALE1
0.017020
0.000000
0.000000
0.00000


SCALE2
0.000000
0.014962
0.000000
0.00000


SCALE3
0.000000
0.000000
0.009339
0.00000


















ATOM
1
N
ALA
A
1
2.336
20.870
1.027
1.00
27.48
N


ATOM
3
CA
ALA
A
1
1.951
20.940
2.465
1.00
29.42
C


ATOM
5
CB
ALA
A
1
2.391
19.637
3.197
1.00
29.45
C


ATOM
9
C
ALA
A
1
2.665
22.149
3.096
1.00
28.87
C


ATOM
10
O
ALA
A
1
3.696
22.577
2.627
1.00
30.49
O


ATOM
13
N
VAL
A
2
2.014
22.747
4.052
1.00
30.31
N


ATOM
15
CA
VAL
A
2
2.658
23.754
4.877
1.00
30.69
C


ATOM
17
CB
VAL
A
2
2.068
25.139
4.604
1.00
30.94
C


ATOM
19
CG1
VAL
A
2
2.611
25.702
3.252
1.00
32.62
C


ATOM
23
CG2
VAL
A
2
0.577
25.086
4.667
1.00
30.84
C


ATOM
27
C
VAL
A
2
2.494
23.346
6.347
1.00
29.48
C


ATOM
28
O
VAL
A
2
1.580
22.610
6.743
1.00
29.33
O


ATOM
29
N
PRO
A
3
3.412
23.788
7.186
1.00
28.10
N


ATOM
30
CA
PRO
A
3
3.298
23.380
8.581
1.00
27.90
C


ATOM
32
CB
PRO
A
3
4.645
23.830
9.185
1.00
26.48
C


ATOM
35
CG
PRO
A
3
5.116
24.998
8.340
1.00
26.57
C


ATOM
38
CD
PRO
A
3
4.530
24.697
6.933
1.00
27.60
C


ATOM
41
C
PRO
A
3
2.129
24.112
9.216
1.00
27.01
C


ATOM
42
O
PRO
A
3
1.602
25.037
8.600
1.00
28.48
O


ATOM
43
N
SER
A
4
1.767
23.774
10.434
1.00
26.37
N


ATOM
45
CA
SER
A
4
0.718
24.505
11.159
1.00
25.29
C


ATOM
47
CB
SER
A
4
0.279
23.780
12.444
1.00
26.52
C


ATOM
50
OG
SER
A
4
1.173
23.913
13.554
1.00
25.41
O


ATOM
52
C
SER
A
4
1.105
25.956
11.445
1.00
26.09
C


ATOM
53
O
SER
A
4
0.212
26.810
11.603
1.00
25.32
O


ATOM
54
N
THR
A
5
2.415
26.233
11.497
1.00
24.12
N


ATOM
56
CA
THR
A
5
3.023
27.567
11.741
1.00
22.81
C


ATOM
58
CB
THR
A
5
3.009
28.007
13.237
1.00
24.21
C


ATOM
60
OG1
THR
A
5
3.793
29.191
13.386
1.00
23.29
O


ATOM
62
CG2
THR
A
5
3.727
26.999
14.151
1.00
22.71
C


ATOM
66
C
THR
A
5
4.413
27.436
11.219
1.00
23.10
C


ATOM
67
O
THR
A
5
5.012
26.322
11.267
1.00
21.48
O


ATOM
68
N
GLN
A
6
4.959
28.518
10.692
1.00
21.30
N


ATOM
70
CA
GLN
A
6
6.260
28.438
10.102
1.00
21.50
C


ATOM
72
CB
GLN
A
6
6.512
29.634
9.209
1.00
22.77
C


ATOM
75
CG
GLN
A
6
5.626
29.570
7.926
1.00
23.15
C


ATOM
78
CD
GLN
A
6
5.999
30.579
6.911
1.00
28.40
C


ATOM
79
OE1
GLN
A
6
5.356
31.619
6.822
1.00
30.34
O


ATOM
80
NE2
GLN
A
6
7.016
30.300
6.133
1.00
24.59
N


ATOM
83
C
GLN
A
6
7.295
28.389
11.185
1.00
20.24
C


ATOM
84
O
GLN
A
6
8.438
28.017
10.927
1.00
18.46
O


ATOM
85
N
THR
A
7
6.870
28.777
12.378
1.00
18.84
N


ATOM
87
CA
THR
A
7
7.752
28.808
13.565
1.00
19.27
C


ATOM
89
CB
THR
A
7
8.135
30.238
13.914
1.00
19.55
C


ATOM
91
OG1
THR
A
7
6.958
31.041
14.091
1.00
23.00
O


ATOM
93
CG2
THR
A
7
8.910
30.878
12.842
1.00
19.61
C


ATOM
97
C
THR
A
7
7.111
28.128
14.755
1.00
18.28
C


ATOM
98
O
THR
A
7
6.436
28.735
15.547
1.00
18.57
O


ATOM
99
N
PRO
A
8
7.288
26.803
14.834
1.00
17.34
N


ATOM
100
CA
PRO
A
8
6.795
26.000
15.922
1.00
17.79
C


ATOM
102
CB
PRO
A
8
7.459
24.615
15.659
1.00
17.18
C


ATOM
105
CG
PRO
A
8
7.556
24.570
14.138
1.00
18.32
C


ATOM
108
CD
PRO
A
8
7.961
25.984
13.814
1.00
16.59
C


ATOM
111
C
PRO
A
8
7.162
26.584
17.273
1.00
16.99
C


ATOM
112
O
PRO
A
8
8.105
27.339
17.369
1.00
18.02
O


ATOM
113
N
TRP
A
9
6.426
26.203
18.280
1.00
18.21
N


ATOM
115
CA
TRP
A
9
6.613
26.750
19.611
1.00
17.56
C


ATOM
117
CB
TRP
A
9
5.723
26.059
20.603
1.00
17.84
C


ATOM
120
CG
TRP
A
9
6.129
24.806
21.197
1.00
14.36
C


ATOM
121
CD1
TRP
A
9
5.772
23.569
20.796
1.00
15.37
C


ATOM
123
NE1
TRP
A
9
6.278
22.630
21.658
1.00
15.66
N


ATOM
125
CE2
TRP
A
9
7.033
23.276
22.609
1.00
16.83
C


ATOM
126
CD2
TRP
A
9
6.952
24.642
22.345
1.00
14.81
C


ATOM
127
CE3
TRP
A
9
7.642
25.531
23.186
1.00
14.62
C


ATOM
129
CZ3
TRP
A
9
8.362
24.982
24.301
1.00
11.92
C


ATOM
131
CH2
TRP
A
9
8.393
23.626
24.526
1.00
15.97
C


ATOM
133
CZ2
TRP
A
9
7.757
22.750
23.677
1.00
14.67
C


ATOM
135
C
TRP
A
9
8.073
26.760
20.083
1.00
18.17
C


ATOM
136
O
TRP
A
9
8.531
27.737
20.662
1.00
15.91
O


ATOM
137
N
GLY
A
10
8.780
25.675
19.859
1.00
16.36
N


ATOM
139
CA
GLY
A
10
10.180
25.618
20.307
1.00
15.48
C


ATOM
142
C
GLY
A
10
11.108
26.619
19.663
1.00
15.80
C


ATOM
143
O
GLY
A
10
12.089
27.060
20.254
1.00
14.71
O


ATOM
144
N
ILE
A
11
10.801
26.939
18.410
1.00
15.18
N


ATOM
146
CA
ILE
A
11
11.599
27.866
17.642
1.00
15.64
C


ATOM
148
CB
ILE
A
11
11.303
27.768
16.151
1.00
14.65
C


ATOM
150
CG1
ILE
A
11
11.479
26.328
15.653
1.00
15.91
C


ATOM
153
CD1
ILE
A
11
12.945
25.811
15.725
1.00
16.96
C


ATOM
157
CG2
ILE
A
11
12.204
28.704
15.385
1.00
16.09
C


ATOM
161
C
ILE
A
11
11.291
29.225
18.193
1.00
15.73
C


ATOM
162
O
ILE
A
11
12.197
29.995
18.438
1.00
15.02
O


ATOM
163
N
LYS
A
12
10.005
29.552
18.352
1.00
16.62
N


ATOM
165
CA
LYS
A
12
9.649
30.832
18.949
1.00
16.28
C


ATOM
167
CB
LYS
A
12
8.147
30.926
19.078
1.00
17.21
C


ATOM
170
CG
LYS
A
12
7.419
31.148
17.707
1.00
20.12
C


ATOM
173
CD
LYS
A
12
5.874
31.303
17.997
1.00
20.50
C


ATOM
176
CE
LYS
A
12
5.137
31.855
16.795
1.00
28.93
C


ATOM
179
NZ
LYS
A
12
4.564
30.819
15.858
1.00
19.42
N


ATOM
183
C
LYS
A
12
10.242
30.956
20.345
1.00
15.82
C


ATOM
184
O
LYS
A
12
10.790
32.014
20.745
1.00
15.87
O


ATOM
185
N
SER
A
13
10.172
29.865
21.075
1.00
13.09
N


ATOM
187
CA
SER
A
13
10.655
29.893
22.450
1.00
13.64
C


ATOM
189
CB
SER
A
13
10.284
28.586
23.149
1.00
12.46
C


ATOM
192
OG
SER
A
13
10.790
28.548
24.491
1.00
14.12
O


ATOM
194
C
SER
A
13
12.167
30.131
22.519
1.00
12.81
C


ATOM
195
O
SER
A
13
12.650
30.959
23.323
1.00
11.51
O


ATOM
196
N
ILE
A
14
12.931
29.371
21.752
1.00
12.90
N


ATOM
198
CA
ILE
A
14
14.391
29.521
21.839
1.00
13.05
C


ATOM
200
CB
ILE
A
14
15.108
28.318
21.206
1.00
12.73
C


ATOM
202
CG1
ILE
A
14
16.498
28.183
21.810
1.00
13.73
C


ATOM
205
CD1
ILE
A
14
17.265
26.959
21.415
1.00
17.32
C


ATOM
209
CG2
ILE
A
14
15.161
28.394
19.753
1.00
14.13
C


ATOM
213
C
ILE
A
14
14.869
30.861
21.299
1.00
14.41
C


ATOM
214
O
ILE
A
14
15.907
31.367
21.680
1.00
15.23
O


ATOM
215
N
TYR
A
15
14.094
31.423
20.389
1.00
15.25
N


ATOM
217
CA
TYR
A
15
14.392
32.753
19.877
1.00
16.87
C


ATOM
219
CB
TYR
A
15
13.742
32.965
18.490
1.00
15.13
C


ATOM
222
CG
TYR
A
15
14.683
32.629
17.348
1.00
16.40
C


ATOM
223
CD1
TYR
A
15
14.956
31.303
17.008
1.00
14.01
C


ATOM
225
CE1
TYR
A
15
15.834
30.998
16.024
1.00
16.36
C


ATOM
227
CZ
TYR
A
15
16.453
31.993
15.321
1.00
16.82
C


ATOM
228
OH
TYR
A
15
17.364
31.738
14.329
1.00
15.20
O


ATOM
230
CE2
TYR
A
15
16.182
33.303
15.621
1.00
17.27
C


ATOM
232
CD2
TYR
A
15
15.320
33.610
16.628
1.00
16.64
C


ATOM
234
C
TYR
A
15
13.925
33.826
20.856
1.00
16.27
C


ATOM
235
O
TYR
A
15
14.311
35.008
20.744
1.00
17.84
O


ATOM
236
N
ASN
A
16
13.075
33.432
21.780
1.00
17.21
N


ATOM
238
CA
ASN
A
16
12.534
34.334
22.811
1.00
17.53
C


ATOM
240
CB
ASN
A
16
13.628
34.860
23.743
1.00
16.83
C


ATOM
243
CG
ASN
A
16
13.098
35.265
25.103
1.00
18.27
C


ATOM
244
OD1
ASN
A
16
11.901
35.461
25.288
1.00
21.90
O


ATOM
245
ND2
ASN
A
16
13.987
35.324
26.075
1.00
17.31
N


ATOM
248
C
ASN
A
16
11.788
35.480
22.114
1.00
19.04
C


ATOM
249
O
ASN
A
16
11.940
36.642
22.463
1.00
18.19
O


ATOM
250
N
ASP
A
17
10.972
35.107
21.135
1.00
18.56
N


ATOM
252
CA
ASP
A
17
10.176
36.069
20.372
1.00
19.69
C


ATOM
254
CB
ASP
A
17
11.019
36.634
19.287
1.00
19.45
C


ATOM
257
CG
ASP
A
17
10.362
37.812
18.579
1.00
20.65
C


ATOM
258
OD1
ASP
A
17
9.160
38.017
18.745
1.00
24.61
O


ATOM
259
OD2
ASP
A
17
11.034
38.547
17.849
1.00
19.73
O


ATOM
260
C
ASP
A
17
8.937
35.412
19.778
1.00
19.80
C


ATOM
261
O
ASP
A
17
9.032
34.693
18.834
1.00
22.05
O


ATOM
262
N
GLN
A
18
7.791
35.647
20.369
1.00
20.51
N


ATOM
264
CA
GLN
A
18
6.593
34.957
19.960
1.00
21.49
C


ATOM
266
CB
GLN
A
18
5.549
35.062
21.054
1.00
22.51
C


ATOM
269
CG
GLN
A
18
5.917
34.348
22.318
1.00
24.55
C


ATOM
272
CD
GLN
A
18
6.243
32.907
22.041
1.00
29.84
C


ATOM
273
OE1
GLN
A
18
7.347
32.450
22.314
1.00
31.44
O


ATOM
274
NE2
GLN
A
18
5.301
32.204
21.457
1.00
28.00
N


ATOM
277
C
GLN
A
18
6.076
35.511
18.658
1.00
21.92
C


ATOM
278
O
GLN
A
18
5.213
34.911
18.021
1.00
22.98
O


ATOM
279
N
SER
A
19
6.697
36.572
18.185
1.00
22.89
N


ATOM
281
CA
SER
A
19
6.231
37.202
16.951
1.00
23.92
C


ATOM
283
CB
SER
A
19
6.338
38.715
17.096
1.00
23.69
C


ATOM
286
OG
SER
A
19
7.627
39.225
16.746
1.00
25.66
O


ATOM
288
C
SER
A
19
6.947
36.728
15.670
1.00
24.30
C


ATOM
289
O
SER
A
19
6.454
36.972
14.566
1.00
25.69
O


ATOM
290
N
ILE
A
20
8.079
36.029
15.764
1.00
23.62
N


ATOM
292
CA
ILE
A
20
8.791
35.673
14.529
1.00
23.92
C


ATOM
294
CB
ILE
A
20
10.104
34.965
14.836
1.00
24.53
C


ATOM
296
CG1
ILE
A
20
9.858
33.755
15.727
1.00
22.48
C


ATOM
299
CD1
ILE
A
20
11.041
32.826
15.641
1.00
23.83
C


ATOM
303
CG2
ILE
A
20
11.127
35.902
15.477
1.00
27.13
C


ATOM
307
C
ILE
A
20
8.011
34.784
13.573
1.00
23.40
C


ATOM
308
O
ILE
A
20
7.241
33.913
13.999
1.00
24.30
O


ATOM
309
N
THR
A
21
8.295
34.963
12.296
1.00
23.97
N


ATOM
311
CA
THR
A
21
7.686
34.184
11.215
1.00
24.95
C


ATOM
313
CB
THR
A
21
6.856
35.107
10.283
1.00
24.97
C


ATOM
315
OG1
THR
A
21
7.690
36.186
9.842
1.00
27.61
O


ATOM
317
CG2
THR
A
21
5.771
35.794
11.039
1.00
28.02
C


ATOM
321
C
THR
A
21
8.799
33.570
10.392
1.00
23.92
C


ATOM
322
O
THR
A
21
8.544
32.865
9.419
1.00
23.90
O


ATOM
323
N
LYS
A
22
10.044
33.862
10.735
1.00
23.93
N


ATOM
325
CA
LYS
A
22
11.148
33.239
10.041
1.00
24.18
C


ATOM
327
CB
LYS
A
22
11.386
33.880
8.675
1.00
25.22
C


ATOM
330
CG
LYS
A
22
11.830
35.314
8.724
1.00
28.95
C


ATOM
333
CD
LYS
A
22
12.397
35.808
7.348
1.00
32.55
C


ATOM
336
CE
LYS
A
22
13.701
35.129
6.988
0.10
31.56
C


ATOM
339
NZ
LYS
A
22
14.274
35.671
5.722
0.10
31.66
N


ATOM
343
C
LYS
A
22
12.406
33.333
10.903
1.00
23.63
C


ATOM
344
O
LYS
A
22
12.471
34.145
11.823
1.00
24.17
O


ATOM
345
N
THR
A
23
13.395
32.491
10.628
1.00
21.41
N


ATOM
347
CA
THR
A
23
14.661
32.526
11.342
1.00
20.21
C


ATOM
349
CB
THR
A
23
14.914
31.202
12.030
1.00
20.28
C


ATOM
351
OG1
THR
A
23
14.859
30.158
11.034
1.00
19.28
O


ATOM
353
CG2
THR
A
23
13.846
30.915
13.074
1.00
21.44
C


ATOM
357
C
THR
A
23
15.785
32.791
10.384
1.00
20.28
C


ATOM
358
O
THR
A
23
15.565
32.740
9.182
1.00
19.80
O


ATOM
359
N
THR
A
24
16.996
33.027
10.908
1.00
19.65
N


ATOM
361
CA
THR
A
24
18.201
33.246
10.130
1.00
19.91
C


ATOM
363
CB
THR
A
24
18.532
34.784
9.840
1.00
21.93
C


ATOM
365
OG1
THR
A
24
18.685
35.435
11.102
1.00
25.69
O


ATOM
367
CG2
THR
A
24
17.402
35.514
9.229
1.00
25.02
C


ATOM
371
C
THR
A
24
19.407
32.743
10.928
1.00
19.55
C


ATOM
372
O
THR
A
24
19.372
32.551
12.149
1.00
19.70
O


ATOM
373
N
GLY
A
25
20.473
32.497
10.225
1.00
17.41
N


ATOM
375
CA
GLY
A
25
21.716
32.187
10.893
1.00
17.64
C


ATOM
378
C
GLY
A
25
22.286
30.799
10.641
1.00
16.16
C


ATOM
379
O
GLY
A
25
21.583
29.936
10.124
1.00
15.92
O


ATOM
380
N
GLY
A
26
23.548
30.620
11.068
1.00
15.49
N


ATOM
382
CA
GLY
A
26
24.296
29.380
10.938
1.00
15.44
C


ATOM
385
C
GLY
A
26
25.166
29.220
9.692
1.00
15.61
C


ATOM
386
O
GLY
A
26
25.782
28.199
9.493
1.00
15.36
O


ATOM
387
N
SER
A
27
25.264
30.249
8.861
1.00
17.72
N


ATOM
389
CA
SER
A
27
26.108
30.182
7.691
1.00
18.45
C


ATOM
391
CB
SER
A
27
25.990
31.500
6.837
1.00
20.08
C


ATOM
394
OG
SER
A
27
26.686
32.496
7.490
1.00
26.34
O


ATOM
396
C
SER
A
27
27.534
29.838
8.029
1.00
17.45
C


ATOM
397
O
SER
A
27
28.166
30.341
8.969
1.00
17.59
O


ATOM
398
N
GLY
A
28
28.071
28.913
7.241
1.00
16.01
N


ATOM
400
CA
GLY
A
28
29.421
28.494
7.385
1.00
16.46
C


ATOM
403
C
GLY
A
28
29.615
27.360
8.377
1.00
15.36
C


ATOM
404
O
GLY
A
28
30.739
26.917
8.527
1.00
16.82
O


ATOM
405
N
ILE
A
29
28.587
26.931
9.076
1.00
14.25
N


ATOM
407
CA
ILE
A
29
28.782
25.832
10.044
1.00
13.92
C


ATOM
409
CB
ILE
A
29
28.057
26.170
11.335
1.00
14.10
C


ATOM
411
CG1
ILE
A
29
28.482
27.563
11.856
1.00
13.61
C


ATOM
414
CD1
ILE
A
29
29.986
27.710
12.081
1.00
15.86
C


ATOM
418
CG2
ILE
A
29
28.269
25.076
12.417
1.00
14.40
C


ATOM
422
C
ILE
A
29
28.143
24.586
9.459
1.00
14.30
C


ATOM
423
O
ILE
A
29
27.190
24.708
8.690
1.00
14.55
O


ATOM
424
N
LYS
A
30
28.614
23.402
9.853
1.00
13.73
N


ATOM
426
CA
LYS
A
30
28.008
22.173
9.422
1.00
13.73
C


ATOM
428
CB
LYS
A
30
29.019
21.204
8.860
1.00
14.64
C


ATOM
431
CG
LYS
A
30
30.072
21.822
7.951
1.00
15.03
C


ATOM
434
CD
LYS
A
30
29.438
22.566
6.745
1.00
16.42
C


ATOM
437
CE
LYS
A
30
30.497
23.364
5.987
1.00
19.44
C


ATOM
440
NZ
LYS
A
30
29.865
24.009
4.752
1.00
17.01
N


ATOM
444
C
LYS
A
30
27.354
21.482
10.655
1.00
13.57
C


ATOM
445
O
LYS
A
30
27.978
21.473
11.716
1.00
14.48
O


ATOM
446
N
VAL
A
31
26.163
20.941
10.498
1.00
13.11
N


ATOM
448
CA
VAL
A
31
25.572
20.118
11.583
1.00
13.65
C


ATOM
450
CB
VAL
A
31
24.249
20.639
12.061
1.00
13.16
C


ATOM
452
CG1
VAL
A
31
23.726
19.743
13.173
1.00
15.78
C


ATOM
456
CG2
VAL
A
31
24.401
22.058
12.573
1.00
12.55
C


ATOM
460
C
VAL
A
31
25.470
18.708
11.047
1.00
14.30
C


ATOM
461
O
VAL
A
31
24.828
18.458
10.003
1.00
14.74
O


ATOM
462
N
ALA
A
32
26.129
17.789
11.737
1.00
14.62
N


ATOM
464
CA
ALA
A
32
26.092
16.387
11.366
1.00
13.46
C


ATOM
466
CB
ALA
A
32
27.419
15.728
11.606
1.00
11.29
C


ATOM
470
C
ALA
A
32
24.972
15.696
12.149
1.00
13.08
C


ATOM
471
O
ALA
A
32
25.056
15.556
13.393
1.00
12.55
O


ATOM
472
N
VAL
A
33
23.916
15.340
11.435
1.00
10.76
N


ATOM
474
CA
VAL
A
33
22.778
14.654
12.038
1.00
11.75
C


ATOM
476
CB
VAL
A
33
21.468
15.152
11.453
1.00
11.00
C


ATOM
478
CG1
VAL
A
33
20.317
14.313
11.935
1.00
13.80
C


ATOM
482
CG2
VAL
A
33
21.268
16.618
11.738
1.00
12.19
C


ATOM
486
C
VAL
A
33
22.959
13.155
11.847
1.00
12.72
C


ATOM
487
O
VAL
A
33
22.830
12.623
10.715
1.00
11.99
O


ATOM
488
N
LEU
A
34
23.290
12.466
12.932
1.00
12.34
N


ATOM
490
CA
LEU
A
34
23.599
11.022
12.949
1.00
11.72
C


ATOM
492
CB
LEU
A
34
24.811
10.744
13.878
1.00
11.57
C


ATOM
495
CG
LEU
A
34
26.190
10.819
13.262
1.00
11.59
C


ATOM
497
CD1
LEU
A
34
26.515
12.228
12.621
1.00
10.38
C


ATOM
501
CD2
LEU
A
34
27.275
10.414
14.265
1.00
12.17
C


ATOM
505
C
LEU
A
34
22.303
10.366
13.416
1.00
12.33
C


ATOM
506
O
LEU
A
34
21.964
10.409
14.581
1.00
11.53
O


ATOM
507
N
ASP
A
35
21.571
9.765
12.490
1.00
11.34
N


ATOM
509
CA
ASP
A
35
20.224
9.416
12.794
1.00
11.07
C


ATOM
511
CB
ASP
A
35
19.397
10.677
12.779
1.00
12.91
C


ATOM
514
CG
ASP
A
35
18.231
10.611
13.697
1.00
11.93
C


ATOM
515
OD1
ASP
A
35
17.334
9.791
13.472
1.00
12.81
O


ATOM
516
OD2
ASP
A
35
18.166
11.390
14.700
1.00
15.59
O


ATOM
517
C
ASP
A
35
19.687
8.393
11.816
1.00
12.34
C


ATOM
518
O
ASP
A
35
20.470
7.623
11.250
1.00
12.33
O


ATOM
519
N
THR
A
36
18.376
8.385
11.604
1.00
12.33
N


ATOM
521
CA
THR
A
36
17.783
7.395
10.702
1.00
13.86
C


ATOM
523
CB
THR
A
36
16.312
7.198
10.972
1.00
13.36
C


ATOM
525
OG1
THR
A
36
15.603
8.446
10.753
1.00
14.42
O


ATOM
527
CG2
THR
A
36
16.058
6.781
12.383
1.00
11.98
C


ATOM
531
C
THR
A
36
17.933
7.710
9.199
1.00
14.93
C


ATOM
532
O
THR
A
36
17.341
7.023
8.379
1.00
15.02
O


ATOM
533
N
GLY
A
37
18.699
8.735
8.885
1.00
15.27
N


ATOM
535
CA
GLY
A
37
18.838
9.282
7.530
1.00
15.54
C


ATOM
538
C
GLY
A
37
18.041
10.594
7.487
1.00
16.06
C


ATOM
539
O
GLY
A
37
17.413
10.973
8.482
1.00
14.08
O


ATOM
540
N
VAL
A
38
18.065
11.292
6.337
1.00
16.27
N


ATOM
542
CA
VAL
A
38
17.315
12.547
6.177
1.00
15.20
C


ATOM
544
CB
VAL
A
38
18.214
13.784
6.447
1.00
15.83
C


ATOM
546
CG1
VAL
A
38
17.501
15.075
6.182
1.00
16.87
C


ATOM
550
CG2
VAL
A
38
18.774
13.766
7.839
1.00
17.47
C


ATOM
554
C
VAL
A
38
16.863
12.628
4.695
1.00
16.04
C


ATOM
555
O
VAL
A
38
17.622
12.277
3.793
1.00
14.66
O


ATOM
556
N
TYR
A
39
15.623
13.008
4.532
1.00
18.21
N


ATOM
558
CA
TYR
A
39
15.046
13.247
3.214
1.00
19.90
C


ATOM
560
CB
TYR
A
39
13.564
13.150
3.366
1.00
18.60
C


ATOM
563
CG
TYR
A
39
12.795
13.480
2.082
1.00
23.59
C


ATOM
564
CD1
TYR
A
39
13.278
13.110
0.833
1.00
27.19
C


ATOM
566
CE1
TYR
A
39
12.555
13.413
−0.309
1.00
30.38
C


ATOM
568
CZ
TYR
A
39
11.391
14.129
−0.226
1.00
31.18
C


ATOM
569
OH
TYR
A
39
10.734
14.412
−1.434
1.00
30.34
O


ATOM
571
CE2
TYR
A
39
10.912
14.528
1.005
1.00
29.74
C


ATOM
573
CD2
TYR
A
39
11.623
14.208
2.144
1.00
23.70
C


ATOM
575
C
TYR
A
39
15.495
14.623
2.786
1.00
19.47
C


ATOM
576
O
TYR
A
39
14.795
15.631
2.992
1.00
22.39
O


ATOM
577
N
THR
A
40
16.675
14.659
2.240
1.00
22.23
N


ATOM
579
CA
THR
A
40
17.366
15.869
1.904
1.00
23.84
C


ATOM
581
CB
THR
A
40
18.797
15.520
1.499
1.00
25.17
C


ATOM
583
OG1
THR
A
40
18.841
14.473
0.518
1.00
27.20
O


ATOM
585
CG2
THR
A
40
19.633
14.890
2.687
1.00
23.66
C


ATOM
589
C
THR
A
40
16.650
16.659
0.804
1.00
25.31
C


ATOM
590
O
THR
A
40
17.008
17.803
0.566
1.00
25.93
O


ATOM
591
N
SER
A
41
15.671
16.051
0.147
1.00
25.63
N


ATOM
593
CA
SER
A
41
14.953
16.703
−0.942
1.00
26.05
C


ATOM
595
CB
SER
A
41
14.662
15.676
−2.047
1.00
25.60
C


ATOM
598
OG
SER
A
41
15.836
15.411
−2.759
1.00
26.14
O


ATOM
600
C
SER
A
41
13.669
17.317
−0.445
1.00
26.09
C


ATOM
601
O
SER
A
41
12.896
17.889
−1.232
1.00
26.66
O


ATOM
602
N
HIS
A
42
13.366
17.179
0.857
1.00
22.90
N


ATOM
604
CA
HIS
A
42
12.245
17.917
1.419
1.00
21.28
C


ATOM
606
CB
HIS
A
42
12.224
17.792
2.927
1.00
21.28
C


ATOM
609
CG
HIS
A
42
10.988
18.267
3.562
1.00
18.79
C


ATOM
610
ND1
HIS
A
42
10.616
19.591
3.556
1.00
17.72
N


ATOM
612
CE1
HIS
A
42
9.482
19.706
4.197
1.00
14.42
C


ATOM
614
NE2
HIS
A
42
9.124
18.516
4.654
1.00
18.07
N


ATOM
616
CD2
HIS
A
42
10.028
17.601
4.230
1.00
15.85
C


ATOM
618
C
HIS
A
42
12.427
19.379
1.036
1.00
20.43
C


ATOM
619
O
HIS
A
42
13.543
19.890
1.077
1.00
19.85
O


ATOM
620
N
LEU
A
43
11.326
20.044
0.686
1.00
21.20
N


ATOM
622
CA
LEU
A
43
11.380
21.409
0.210
1.00
21.25
C


ATOM
624
CB
LEU
A
43
10.030
21.945
−0.087
1.00
22.55
C


ATOM
627
CG
LEU
A
43
9.448
21.512
−1.433
1.00
25.81
C


ATOM
629
CD1
LEU
A
43
8.021
21.976
−1.464
1.00
27.58
C


ATOM
633
CD2
LEU
A
43
10.234
22.108
−2.559
1.00
27.86
C


ATOM
637
C
LEU
A
43
12.023
22.311
1.227
1.00
21.05
C


ATOM
638
O
LEU
A
43
12.699
23.255
0.879
1.00
18.87
O


ATOM
639
N
ASP
A
44
11.847
22.042
2.500
1.00
21.71
N


ATOM
641
CA
ASP
A
44
12.514
22.885
3.487
1.00
20.87
C


ATOM
643
CB
ASP
A
44
11.642
22.917
4.719
1.00
21.17
C


ATOM
646
CG
ASP
A
44
10.262
23.417
4.441
1.00
23.00
C


ATOM
647
OD1
ASP
A
44
10.060
24.154
3.406
1.00
21.93
O


ATOM
648
OD2
ASP
A
44
9.325
23.156
5.206
1.00
16.28
O


ATOM
649
C
ASP
A
44
13.962
22.528
3.812
1.00
21.02
C


ATOM
650
O
ASP
A
44
14.593
23.214
4.604
1.00
18.05
O


ATOM
651
N
LEU
A
45
14.488
21.431
3.252
1.00
18.30
N


ATOM
653
CA
LEU
A
45
15.868
21.070
3.445
1.00
19.90
C


ATOM
655
CB
LEU
A
45
15.922
19.624
4.024
1.00
18.80
C


ATOM
658
CG
LEU
A
45
15.174
19.394
5.300
1.00
18.65
C


ATOM
660
CD1
LEU
A
45
15.424
17.925
5.756
1.00
18.05
C


ATOM
664
CD2
LEU
A
45
15.714
20.351
6.357
1.00
20.33
C


ATOM
668
C
LEU
A
45
16.750
21.110
2.197
1.00
20.37
C


ATOM
669
O
LEU
A
45
17.960
20.890
2.251
1.00
22.13
O


ATOM
670
N
ALA
A
46
16.104
21.400
1.079
1.00
22.51
N


ATOM
672
CA
ALA
A
46
16.728
21.327
−0.210
1.00
21.13
C


ATOM
674
CB
ALA
A
46
15.738
21.695
−1.365
1.00
21.15
C


ATOM
678
C
ALA
A
46
17.880
22.199
−0.244
1.00
20.28
C


ATOM
679
O
ALA
A
46
17.806
23.344
0.177
1.00
22.66
O


ATOM
680
N
GLY
A
47
18.959
21.639
−0.759
1.00
20.67
N


ATOM
682
CA
GLY
A
47
20.217
22.320
−0.968
1.00
21.84
C


ATOM
685
C
GLY
A
47
21.042
22.419
0.291
1.00
21.53
C


ATOM
686
O
GLY
A
47
22.226
22.881
0.280
1.00
24.34
O


ATOM
687
N
SER
A
48
20.522
21.910
1.392
1.00
22.52
N


ATOM
689
CA
SER
A
48
21.310
21.980
2.610
1.00
21.98
C


ATOM
691
CB
ASER
A
48
20.384
22.083
3.833
0.50
22.54
C


ATOM
692
CB
BSER
A
48
20.408
22.073
3.814
0.50
22.26
C


ATOM
697
OG
ASER
A
48
19.449
21.001
3.944
0.50
23.88
O


ATOM
698
OG
BSER
A
48
19.660
23.258
3.738
0.50
21.10
O


ATOM
701
C
SER
A
48
22.295
20.852
2.826
1.00
21.47
C


ATOM
702
O
SER
A
48
23.317
21.066
3.466
1.00
21.64
O


ATOM
703
N
ALA
A
49
22.035
19.670
2.285
1.00
20.96
N


ATOM
705
CA
ALA
A
49
22.951
18.532
2.483
1.00
22.38
C


ATOM
707
CB
ALA
A
49
22.192
17.247
2.068
1.00
22.64
C


ATOM
711
C
ALA
A
49
24.233
18.644
1.718
1.00
23.21
C


ATOM
712
O
ALA
A
49
24.228
18.551
0.500
1.00
26.19
O


ATOM
713
N
GLU
A
50
25.328
18.866
2.426
1.00
20.40
N


ATOM
715
CA
GLU
A
50
26.598
18.826
1.753
1.00
21.76
C


ATOM
717
CB
GLU
A
50
27.625
19.892
2.250
1.00
22.32
C


ATOM
720
CG
GLU
A
50
27.374
21.244
1.591
1.00
26.62
C


ATOM
723
CD
GLU
A
50
28.046
22.421
2.279
1.00
30.59
C


ATOM
724
OE1
GLU
A
50
28.886
22.227
3.181
1.00
24.43
O


ATOM
725
OE2
GLU
A
50
27.683
23.561
1.918
1.00
35.88
O


ATOM
726
C
GLU
A
50
27.208
17.435
1.866
1.00
20.83
C


ATOM
727
O
GLU
A
50
28.257
17.188
1.220
1.00
21.81
O


ATOM
728
N
GLN
A
51
26.761
16.586
2.783
1.00
19.08
N


ATOM
730
CA
GLN
A
51
27.273
15.186
2.847
1.00
18.38
C


ATOM
732
CB
GLN
A
51
28.416
14.936
3.863
1.00
18.98
C


ATOM
735
CG
GLN
A
51
29.720
15.707
3.698
1.00
16.56
C


ATOM
738
CD
GLN
A
51
30.864
15.082
4.418
1.00
18.35
C


ATOM
739
OE1
GLN
A
51
30.728
14.001
4.993
1.00
16.59
O


ATOM
740
NE2
GLN
A
51
32.021
15.746
4.421
1.00
18.88
N


ATOM
743
C
GLN
A
51
26.048
14.303
3.122
1.00
18.15
C


ATOM
744
O
GLN
A
51
25.088
14.739
3.753
1.00
17.95
O


ATOM
745
N
CYS
A
52
26.032
13.097
2.549
1.00
19.15
N


ATOM
747
CA
CYS
A
52
24.933
12.197
2.657
1.00
19.58
C


ATOM
749
CB
CYS
A
52
23.994
12.383
1.463
1.00
20.58
C


ATOM
752
SG
CYS
A
52
22.757
11.113
1.313
1.00
23.23
S


ATOM
753
C
CYS
A
52
25.609
10.827
2.666
1.00
19.62
C


ATOM
754
O
CYS
A
52
26.112
10.376
1.630
1.00
17.75
O


ATOM
755
N
LYS
A
53
25.706
10.188
3.841
1.00
18.28
N


ATOM
757
CA
LYS
A
53
26.435
8.938
3.934
1.00
17.30
C


ATOM
759
CB
LYS
A
53
27.835
9.165
4.542
1.00
17.03
C


ATOM
762
CG
LYS
A
53
28.733
10.042
3.720
1.00
16.05
C


ATOM
765
CD
LYS
A
53
30.097
10.281
4.325
1.00
17.76
C


ATOM
768
CE
LYS
A
53
31.031
11.033
3.333
1.00
17.09
C


ATOM
771
NZ
LYS
A
53
32.138
11.733
3.893
1.00
19.33
N


ATOM
775
C
LYS
A
53
25.698
7.913
4.801
1.00
18.20
C


ATOM
776
O
LYS
A
53
24.966
8.299
5.712
1.00
15.09
O


ATOM
777
N
ASP
A
54
25.905
6.619
4.518
1.00
15.90
N


ATOM
779
CA
ASP
A
54
25.186
5.563
5.218
1.00
17.38
C


ATOM
781
CB
ASP
A
54
24.244
4.911
4.223
1.00
17.91
C


ATOM
784
CG
ASP
A
54
23.222
3.960
4.825
1.00
20.51
C


ATOM
785
OD1
ASP
A
54
23.261
3.554
6.029
1.00
15.70
O


ATOM
786
OD2
ASP
A
54
22.292
3.563
4.088
1.00
19.49
O


ATOM
787
C
ASP
A
54
26.131
4.552
5.807
1.00
17.88
C


ATOM
788
O
ASP
A
54
26.969
3.968
5.093
1.00
17.08
O


ATOM
789
N
PHE
A
55
25.998
4.356
7.135
1.00
15.74
N


ATOM
791
CA
PHE
A
55
26.865
3.464
7.867
1.00
15.03
C


ATOM
793
CB
PHE
A
55
27.359
4.168
9.131
1.00
13.99
C


ATOM
796
CG
PHE
A
55
28.268
5.336
8.844
1.00
15.45
C


ATOM
797
CD1
PHE
A
55
27.753
6.544
8.432
1.00
15.76
C


ATOM
799
CE1
PHE
A
55
28.616
7.657
8.155
1.00
14.10
C


ATOM
801
CZ
PHE
A
55
29.907
7.536
8.256
1.00
13.98
C


ATOM
803
CE2
PHE
A
55
30.431
6.303
8.656
1.00
15.61
C


ATOM
805
CD2
PHE
A
55
29.594
5.232
8.950
1.00
14.62
C


ATOM
807
C
PHE
A
55
26.160
2.191
8.265
1.00
15.07
C


ATOM
808
O
PHE
A
55
26.732
1.387
9.025
1.00
15.44
O


ATOM
809
N
THR
A
56
24.962
1.994
7.769
1.00
15.31
N


ATOM
811
CA
THR
A
56
24.149
0.858
8.159
1.00
16.77
C


ATOM
813
CB
THR
A
56
22.724
1.253
8.463
1.00
17.17
C


ATOM
815
OG1
THR
A
56
22.006
1.623
7.272
1.00
15.48
O


ATOM
817
CG2
THR
A
56
22.628
2.535
9.443
1.00
13.67
C


ATOM
821
C
THR
A
56
24.134
−0.328
7.166
1.00
20.28
C


ATOM
822
O
THR
A
56
23.451
−1.319
7.407
1.00
21.69
O


ATOM
823
N
GLN
A
57
24.852
−0.239
6.069
1.00
22.81
N


ATOM
825
CA
GLN
A
57
24.736
−1.314
5.061
1.00
25.52
C


ATOM
827
CB
GLN
A
57
24.681
−0.724
3.646
1.00
25.67
C


ATOM
830
CG
GLN
A
57
23.521
0.217
3.502
1.00
27.65
C


ATOM
833
CD
GLN
A
57
23.366
0.800
2.117
1.00
36.31
C


ATOM
834
OE1
GLN
A
57
23.871
0.240
1.156
1.00
35.97
O


ATOM
835
NE2
GLN
A
57
22.686
1.938
2.016
1.00
30.80
N


ATOM
838
C
GLN
A
57
25.848
−2.331
5.196
1.00
28.75
C


ATOM
839
O
GLN
A
57
26.735
−2.182
6.034
1.00
28.60
O


ATOM
840
N
SER
A
58
25.792
−3.388
4.363
1.00
32.27
N


ATOM
842
CA
SER
A
58
26.798
−4.440
4.371
1.00
34.96
C


ATOM
844
CB
SER
A
58
26.488
−5.494
3.291
1.00
35.23
C


ATOM
847
OG
SER
A
58
25.088
−5.548
3.041
1.00
37.60
O


ATOM
849
C
SER
A
58
28.149
−3.762
4.140
1.00
35.56
C


ATOM
850
O
SER
A
58
29.096
−3.989
4.843
1.00
36.58
O


ATOM
851
N
ASN
A
59
28.224
−2.889
3.147
1.00
37.91
N


ATOM
853
CA
ASN
A
59
29.409
−2.054
3.003
1.00
38.41
C


ATOM
855
CB
ASN
A
59
29.288
−1.232
1.739
1.00
39.92
C


ATOM
858
CG
ASN
A
59
30.172
−1.727
0.636
1.00
44.62
C


ATOM
859
OD1
ASN
A
59
31.413
−1.752
0.759
1.00
52.42
O


ATOM
860
ND2
ASN
A
59
29.547
−2.121
−0.468
1.00
50.97
N


ATOM
863
C
ASN
A
59
29.421
−1.061
4.156
1.00
37.64
C


ATOM
864
O
ASN
A
59
28.436
−0.360
4.338
1.00
37.26
O


ATOM
865
N
PRO
A
60
30.474
−1.028
4.961
1.00
37.50
N


ATOM
866
CA
PRO
A
60
30.591
−0.066
6.064
1.00
36.81
C


ATOM
868
CB
PRO
A
60
32.016
−0.315
6.585
1.00
37.80
C


ATOM
871
CG
PRO
A
60
32.661
−1.116
5.519
1.00
38.85
C


ATOM
874
CD
PRO
A
60
31.589
−1.986
4.997
1.00
38.04
C


ATOM
877
C
PRO
A
60
30.421
1.431
5.770
1.00
35.74
C


ATOM
878
O
PRO
A
60
30.266
2.188
6.749
1.00
34.22
O


ATOM
879
N
LEU
A
61
30.478
1.876
4.517
1.00
33.77
N


ATOM
881
CA
LEU
A
61
30.183
3.278
4.258
1.00
33.86
C


ATOM
883
CB
LEU
A
61
31.403
4.170
4.541
1.00
34.63
C


ATOM
886
CG
LEU
A
61
31.122
5.691
4.652
1.00
38.34
C


ATOM
888
CD1
LEU
A
61
32.418
6.454
4.765
1.00
41.14
C


ATOM
892
CD2
LEU
A
61
30.383
6.236
3.501
1.00
40.32
C


ATOM
896
C
LEU
A
61
29.681
3.440
2.838
1.00
31.90
C


ATOM
897
O
LEU
A
61
30.371
3.109
1.887
1.00
32.30
O


ATOM
898
N
VAL
A
62
28.452
3.862
2.682
1.00
29.42
N


ATOM
900
CA
VAL
A
62
27.944
4.129
1.363
1.00
29.40
C


ATOM
902
CB
VAL
A
62
26.721
3.365
1.091
1.00
28.97
C


ATOM
904
CG1
VAL
A
62
26.082
3.877
−0.187
1.00
30.81
C


ATOM
908
CG2
VAL
A
62
27.060
1.874
1.015
1.00
31.48
C


ATOM
912
C
VAL
A
62
27.768
5.625
1.255
1.00
27.76
C


ATOM
913
O
VAL
A
62
27.015
6.233
1.970
1.00
26.96
O


ATOM
914
N
ASP
A
63
28.646
6.224
0.470
1.00
28.18
N


ATOM
916
CA
ASP
A
63
28.600
7.643
0.235
1.00
26.20
C


ATOM
918
CB
ASP
A
63
29.993
8.062
−0.215
1.00
26.90
C


ATOM
921
CG
ASP
A
63
30.222
9.517
−0.099
1.00
25.49
C


ATOM
922
OD1
ASP
A
63
29.290
10.281
−0.017
1.00
26.02
O


ATOM
923
OD2
ASP
A
63
31.318
10.031
−0.150
1.00
28.08
O


ATOM
924
C
ASP
A
63
27.571
7.929
−0.826
1.00
27.79
C


ATOM
925
O
ASP
A
63
27.455
7.199
−1.812
1.00
27.28
O


ATOM
926
N
GLY
A
64
26.753
8.936
−0.581
1.00
26.48
N


ATOM
928
CA
GLY
A
64
25.703
9.316
−1.502
1.00
25.76
C


ATOM
931
C
GLY
A
64
24.357
8.742
−1.234
1.00
25.62
C


ATOM
932
O
GLY
A
64
23.474
8.881
−2.053
1.00
28.14
O


ATOM
933
N
SER
A
65
24.184
8.096
−0.080
1.00
22.83
N


ATOM
935
CA
SER
A
65
22.953
7.499
0.304
1.00
22.85
C


ATOM
937
CB
SER
A
65
23.003
6.005
0.117
1.00
23.23
C


ATOM
940
OG
SER
A
65
21.699
5.584
0.027
1.00
29.80
O


ATOM
942
C
SER
A
65
22.705
7.773
1.749
1.00
21.08
C


ATOM
943
O
SER
A
65
23.671
7.638
2.504
1.00
19.41
O


ATOM
944
N
CYS
A
66
21.521
8.181
2.140
1.00
20.00
N


ATOM
946
CA
CYS
A
66
21.278
8.539
3.546
1.00
20.11
C


ATOM
948
CB
CYS
A
66
22.034
9.822
3.885
1.00
19.63
C


ATOM
951
SG
CYS
A
66
21.484
11.254
2.900
1.00
19.95
S


ATOM
952
C
CYS
A
66
19.803
8.601
3.712
1.00
18.81
C


ATOM
953
O
CYS
A
66
19.168
9.468
4.308
1.00
17.95
O


ATOM
954
N
THR
A
67
19.180
7.568
3.214
1.00
19.39
N


ATOM
956
CA
THR
A
67
17.768
7.596
3.075
1.00
19.72
C


ATOM
958
CB
THR
A
67
17.481
6.628
1.924
1.00
20.77
C


ATOM
960
OG1
THR
A
67
18.082
7.189
0.735
1.00
26.00
O


ATOM
962
CG2
THR
A
67
16.113
6.443
1.665
1.00
23.79
C


ATOM
966
C
THR
A
67
16.941
7.325
4.315
1.00
18.14
C


ATOM
967
O
THR
A
67
17.066
6.297
4.990
1.00
16.62
O


ATOM
968
N
ASP
A
68
16.070
8.278
4.623
1.00
17.54
N


ATOM
970
CA
ASP
A
68
15.191
8.149
5.786
1.00
17.68
C


ATOM
972
CB
ASP
A
68
14.877
9.530
6.360
1.00
16.82
C


ATOM
975
CG
ASP
A
68
14.131
9.480
7.697
1.00
17.49
C


ATOM
976
OD1
ASP
A
68
13.988
8.380
8.314
1.00
14.56
O


ATOM
977
OD2
ASP
A
68
13.610
10.516
8.221
1.00
16.31
O


ATOM
978
C
ASP
A
68
13.909
7.425
5.423
1.00
19.14
C


ATOM
979
O
ASP
A
68
13.100
7.936
4.626
1.00
19.83
O


ATOM
980
N
ARG
A
69
13.688
6.262
6.023
1.00
19.38
N


ATOM
982
CA
ARG
A
69
12.427
5.549
5.858
1.00
20.20
C


ATOM
984
CB
ARG
A
69
12.665
4.106
5.357
1.00
20.57
C


ATOM
987
CG
ARG
A
69
13.461
4.061
4.081
1.00
23.84
C


ATOM
990
CD
ARG
A
69
13.499
2.688
3.401
1.00
28.11
C


ATOM
993
NE
ARG
A
69
14.384
2.688
2.239
1.00
31.97
N


ATOM
995
CZ
ARG
A
69
15.683
2.433
2.284
1.00
34.34
C


ATOM
996
NH1
ARG
A
69
16.288
2.155
3.437
1.00
33.57
N


ATOM
999
NH2
ARG
A
69
16.416
2.464
1.173
1.00
37.78
N


ATOM
1002
C
ARG
A
69
11.615
5.543
7.120
1.00
20.20
C


ATOM
1003
O
ARG
A
69
10.605
4.861
7.222
1.00
19.58
O


ATOM
1004
N
GLN
A
70
12.022
6.341
8.120
1.00
18.56
N


ATOM
1006
CA
GLN
A
70
11.359
6.330
9.404
1.00
18.67
C


ATOM
1008
CB
GLN
A
70
12.459
6.087
10.480
1.00
17.38
C


ATOM
1011
CG
GLN
A
70
11.887
5.512
11.734
1.00
24.45
C


ATOM
1014
CD
GLN
A
70
11.094
6.496
12.618
1.00
29.69
C


ATOM
1015
OE1
GLN
A
70
11.259
7.719
12.568
1.00
28.81
O


ATOM
1016
NE2
GLN
A
70
10.180
5.934
13.390
1.00
36.98
N


ATOM
1019
C
GLN
A
70
10.678
7.677
9.729
1.00
16.99
C


ATOM
1020
O
GLN
A
70
9.502
7.745
10.177
1.00
18.53
O


ATOM
1021
N
GLY
A
71
11.448
8.740
9.546
1.00
17.00
N


ATOM
1023
CA
GLY
A
71
10.936
10.086
9.792
1.00
16.91
C


ATOM
1026
C
GLY
A
71
11.766
10.862
10.826
1.00
16.73
C


ATOM
1027
O
GLY
A
71
12.023
12.040
10.683
1.00
16.08
O


ATOM
1028
N
HIS
A
72
12.190
10.148
11.848
1.00
15.53
N


ATOM
1030
CA
HIS
A
72
12.902
10.764
12.965
1.00
14.82
C


ATOM
1032
CB
HIS
A
72
13.305
9.625
13.926
1.00
14.91
C


ATOM
1035
CG
HIS
A
72
13.996
10.088
15.170
1.00
11.42
C


ATOM
1036
ND1
HIS
A
72
15.356
10.264
15.228
1.00
11.65
N


ATOM
1038
CE1
HIS
A
72
15.690
10.620
16.456
1.00
15.57
C


ATOM
1040
NE2
HIS
A
72
14.603
10.660
17.194
1.00
12.24
N


ATOM
1042
CD2
HIS
A
72
13.527
10.309
16.414
1.00
15.18
C


ATOM
1044
C
HIS
A
72
14.077
11.632
12.515
1.00
14.31
C


ATOM
1045
O
HIS
A
72
14.157
12.811
12.906
1.00
14.46
O


ATOM
1046
N
GLY
A
73
14.993
11.101
11.686
1.00
13.23
N


ATOM
1048
CA
GLY
A
73
16.140
11.851
11.227
1.00
13.67
C


ATOM
1051
C
GLY
A
73
15.743
13.097
10.452
1.00
14.47
C


ATOM
1052
O
GLY
A
73
16.388
14.147
10.556
1.00
14.58
O


ATOM
1053
N
THR
A
74
14.691
12.976
9.638
1.00
14.43
N


ATOM
1055
CA
THR
A
74
14.223
14.163
8.902
1.00
14.75
C


ATOM
1057
CB
THR
A
74
13.166
13.722
7.889
1.00
15.14
C


ATOM
1059
OG1
THR
A
74
13.832
12.851
6.979
1.00
14.14
O


ATOM
1061
CG2
THR
A
74
12.703
14.949
7.052
1.00
17.07
C


ATOM
1065
C
THR
A
74
13.672
15.256
9.779
1.00
13.89
C


ATOM
1066
O
THR
A
74
13.964
16.449
9.549
1.00
14.36
O


ATOM
1067
N
HIS
A
75
12.985
14.834
10.823
1.00
13.97
N


ATOM
1069
CA
HIS
A
75
12.345
15.653
11.803
1.00
13.53
C


ATOM
1071
CB
HIS
A
75
11.464
14.793
12.693
1.00
14.00
C


ATOM
1074
CG
HIS
A
75
10.525
15.543
13.566
1.00
13.88
C


ATOM
1075
ND1
HIS
A
75
10.923
16.209
14.706
1.00
13.19
N


ATOM
1077
CE1
HIS
A
75
9.888
16.830
15.235
1.00
15.22
C


ATOM
1079
NE2
HIS
A
75
8.826
16.616
14.465
1.00
15.33
N


ATOM
1081
CD2
HIS
A
75
9.203
15.822
13.415
1.00
14.61
C


ATOM
1083
C
HIS
A
75
13.464
16.423
12.565
1.00
14.20
C


ATOM
1084
O
HIS
A
75
13.447
17.650
12.685
1.00
11.71
O


ATOM
1085
N
VAL
A
76
14.436
15.685
13.031
1.00
13.61
N


ATOM
1087
CA
VAL
A
76
15.543
16.273
13.761
1.00
13.90
C


ATOM
1089
CB
VAL
A
76
16.471
15.117
14.276
1.00
12.59
C


ATOM
1091
CG1
VAL
A
76
17.771
15.657
14.716
1.00
13.85
C


ATOM
1095
CG2
VAL
A
76
15.788
14.354
15.381
1.00
13.36
C


ATOM
1099
C
VAL
A
76
16.280
17.319
12.925
1.00
13.76
C


ATOM
1100
O
VAL
A
76
16.549
18.419
13.362
1.00
13.81
O


ATOM
1101
N
ALA
A
77
16.598
16.976
11.693
1.00
13.31
N


ATOM
1103
CA
ALA
A
77
17.316
17.850
10.844
1.00
13.43
C


ATOM
1105
CB
ALA
A
77
17.586
17.164
9.553
1.00
12.82
C


ATOM
1109
C
ALA
A
77
16.538
19.154
10.631
1.00
13.42
C


ATOM
1110
O
ALA
A
77
17.137
20.256
10.595
1.00
16.51
O


ATOM
1111
N
GLY
A
78
15.223
19.047
10.501
1.00
13.22
N


ATOM
1113
CA
GLY
A
78
14.413
20.237
10.270
1.00
14.59
C


ATOM
1116
C
GLY
A
78
14.431
21.221
11.448
1.00
14.40
C


ATOM
1117
O
GLY
A
78
14.427
22.440
11.294
1.00
14.85
O


ATOM
1118
N
THR
A
79
14.537
20.673
12.643
1.00
12.64
N


ATOM
1120
CA
THR
A
79
14.546
21.535
13.817
1.00
11.83
C


ATOM
1122
CB
THR
A
79
14.350
20.656
15.063
1.00
11.71
C


ATOM
1124
OG1
THR
A
79
12.990
20.162
15.166
1.00
12.60
O


ATOM
1126
CG2
THR
A
79
14.569
21.491
16.347
1.00
11.10
C


ATOM
1130
C
THR
A
79
15.842
22.248
13.795
1.00
12.20
C


ATOM
1131
O
THR
A
79
15.917
23.440
14.122
1.00
12.14
O


ATOM
1132
N
VAL
A
80
16.917
21.568
13.358
1.00
11.44
N


ATOM
1134
CA
VAL
A
80
18.195
22.225
13.293
1.00
11.80
C


ATOM
1136
CB
VAL
A
80
19.299
21.273
12.865
1.00
11.91
C


ATOM
1138
CG1
VAL
A
80
20.637
21.963
12.687
1.00
13.34
C


ATOM
1142
CG2
VAL
A
80
19.520
20.158
13.884
1.00
12.77
C


ATOM
1146
C
VAL
A
80
18.216
23.369
12.266
1.00
13.39
C


ATOM
1147
O
VAL
A
80
18.646
24.514
12.553
1.00
12.65
O


ATOM
1148
N
LEU
A
81
17.751
23.054
11.069
1.00
13.69
N


ATOM
1150
CA
LEU
A
81
18.057
23.946
9.965
1.00
14.13
C


ATOM
1152
CB
LEU
A
81
19.454
23.675
9.439
1.00
14.13
C


ATOM
1155
CG
LEU
A
81
19.893
22.189
9.225
1.00
11.54
C


ATOM
1157
CD1
LEU
A
81
19.058
21.552
8.105
1.00
15.65
C


ATOM
1161
CD2
LEU
A
81
21.308
22.059
8.854
1.00
14.83
C


ATOM
1165
C
LEU
A
81
17.043
24.065
8.827
1.00
15.14
C


ATOM
1166
O
LEU
A
81
17.442
24.518
7.766
1.00
17.76
O


ATOM
1167
N
ALA
A
82
15.791
23.694
9.035
1.00
15.38
N


ATOM
1169
CA
ALA
A
82
14.830
23.894
7.920
1.00
16.54
C


ATOM
1171
CB
ALA
A
82
13.485
23.412
8.253
1.00
16.56
C


ATOM
1175
C
ALA
A
82
14.807
25.381
7.616
1.00
17.73
C


ATOM
1176
O
ALA
A
82
14.873
26.246
8.522
1.00
16.11
O


ATOM
1177
N
HIS
A
83
14.637
25.678
6.321
1.00
18.61
N


ATOM
1179
CA
HIS
A
83
14.802
27.048
5.845
1.00
17.82
C


ATOM
1181
CB
HIS
A
83
16.057
27.116
4.996
1.00
18.66
C


ATOM
1184
CG
HIS
A
83
16.040
26.187
3.831
1.00
19.55
C


ATOM
1185
ND1
HIS
A
83
14.935
26.066
3.023
1.00
23.39
N


ATOM
1187
CE1
HIS
A
83
15.196
25.205
2.056
1.00
24.12
C


ATOM
1189
NE2
HIS
A
83
16.395
24.706
2.259
1.00
24.21
N


ATOM
1191
CD2
HIS
A
83
16.960
25.326
3.349
1.00
22.73
C


ATOM
1193
C
HIS
A
83
13.606
27.689
5.119
1.00
19.24
C


ATOM
1194
O
HIS
A
83
13.802
28.694
4.468
1.00
20.21
O


ATOM
1195
N
GLY
A
84
12.433
27.158
5.342
1.00
19.88
N


ATOM
1197
CA
GLY
A
84
11.151
27.653
4.874
1.00
23.01
C


ATOM
1200
C
GLY
A
84
10.891
27.373
3.388
1.00
23.83
C


ATOM
1201
O
GLY
A
84
9.816
27.693
2.873
1.00
25.88
O


ATOM
1202
N
GLY
A
85
11.891
26.852
2.716
1.00
25.14
N


ATOM
1204
CA
GLY
A
85
11.754
26.452
1.333
1.00
28.31
C


ATOM
1207
C
GLY
A
85
11.845
27.607
0.361
1.00
30.82
C


ATOM
1208
O
GLY
A
85
11.704
28.777
0.750
1.00
32.32
O


ATOM
1209
N
SER
A
86
12.066
27.253
−0.910
1.00
33.23
N


ATOM
1211
CA
SER
A
86
12.332
28.220
−1.982
1.00
35.37
C


ATOM
1213
CB
SER
A
86
12.287
27.551
−3.374
1.00
35.51
C


ATOM
1216
OG
SER
A
86
11.022
26.920
−3.531
1.00
36.16
O


ATOM
1218
C
SER
A
86
11.323
29.323
−1.984
1.00
35.85
C


ATOM
1219
O
SER
A
86
11.673
30.481
−2.155
1.00
37.71
O


ATOM
1220
N
ASN
A
87
10.066
28.993
−1.784
1.00
36.18
N


ATOM
1222
CA
ASN
A
87
9.060
30.024
−1.844
1.00
37.05
C


ATOM
1224
CB
ASN
A
87
7.842
29.472
−2.589
1.00
37.33
C


ATOM
1227
CG
ASN
A
87
6.943
28.626
−1.702
1.00
40.53
C


ATOM
1228
OD1
ASN
A
87
7.323
28.240
−0.581
1.00
40.83
O


ATOM
1229
ND2
ASN
A
87
5.732
28.329
−2.205
1.00
39.22
N


ATOM
1232
C
ASN
A
87
8.678
30.600
−0.469
1.00
35.97
C


ATOM
1233
O
ASN
A
87
7.564
31.143
−0.295
1.00
36.81
O


ATOM
1234
N
GLY
A
88
9.554
30.402
0.526
1.00
34.13
N


ATOM
1236
CA
GLY
A
88
9.307
30.979
1.841
1.00
32.36
C


ATOM
1239
C
GLY
A
88
8.149
30.556
2.701
1.00
30.27
C


ATOM
1240
O
GLY
A
88
7.882
31.189
3.728
1.00
30.72
O


ATOM
1241
N
GLN
A
89
7.375
29.545
2.305
1.00
28.00
N


ATOM
1243
CA
GLN
A
89
6.212
29.190
3.117
1.00
27.11
C


ATOM
1245
CB
GLN
A
89
4.898
29.091
2.269
1.00
28.51
C


ATOM
1248
CG
GLN
A
89
3.596
28.969
3.114
0.10
26.48
C


ATOM
1251
CD
GLN
A
89
2.269
28.881
2.318
0.10
25.84
C


ATOM
1252
OE1
GLN
A
89
2.243
28.873
1.085
0.10
20.77
O


ATOM
1253
NE2
GLN
A
89
1.164
28.811
3.052
0.10
23.86
N


ATOM
1256
C
GLN
A
89
6.384
27.908
3.974
1.00
26.64
C


ATOM
1257
O
GLN
A
89
5.463
27.490
4.638
1.00
25.73
O


ATOM
1258
N
GLY
A
90
7.572
27.312
3.967
1.00
26.19
N


ATOM
1260
CA
GLY
A
90
7.781
26.104
4.760
1.00
24.89
C


ATOM
1263
C
GLY
A
90
8.133
26.372
6.223
1.00
25.02
C


ATOM
1264
O
GLY
A
90
7.940
27.492
6.751
1.00
25.06
O


ATOM
1265
N
VAL
A
91
8.598
25.330
6.888
1.00
22.83
N


ATOM
1267
CA
VAL
A
91
8.942
25.462
8.304
1.00
20.77
C


ATOM
1269
CB
VAL
A
91
8.681
24.116
9.045
1.00
19.91
C


ATOM
1271
CG1
VAL
A
91
9.781
23.160
8.797
1.00
21.25
C


ATOM
1275
CG2
VAL
A
91
8.463
24.309
10.528
1.00
21.36
C


ATOM
1279
C
VAL
A
91
10.344
25.938
8.411
1.00
18.60
C


ATOM
1280
O
VAL
A
91
11.184
25.738
7.532
1.00
19.96
O


ATOM
1281
N
TYR
A
92
10.632
26.589
9.547
1.00
18.11
N


ATOM
1283
CA
TYR
A
92
11.941
27.076
9.868
1.00
17.39
C


ATOM
1285
CB
TYR
A
92
11.880
28.546
10.256
1.00
16.87
C


ATOM
1288
CG
TYR
A
92
11.827
29.420
9.027
1.00
17.86
C


ATOM
1289
CD1
TYR
A
92
12.989
29.758
8.379
1.00
16.53
C


ATOM
1291
CE1
TYR
A
92
12.993
30.516
7.233
1.00
20.55
C


ATOM
1293
CZ
TYR
A
92
11.793
30.963
6.717
1.00
23.54
C


ATOM
1294
OH
TYR
A
92
11.862
31.737
5.549
1.00
25.77
O


ATOM
1296
CE2
TYR
A
92
10.619
30.672
7.325
1.00
19.89
C


ATOM
1298
CD2
TYR
A
92
10.626
29.840
8.507
1.00
20.04
C


ATOM
1300
C
TYR
A
92
12.542
26.375
11.099
1.00
14.56
C


ATOM
1301
O
TYR
A
92
11.856
26.154
12.042
1.00
14.48
O


ATOM
1302
N
GLY
A
93
13.824
26.133
11.058
1.00
13.49
N


ATOM
1304
CA
GLY
A
93
14.547
25.546
12.182
1.00
15.74
C


ATOM
1307
C
GLY
A
93
15.350
26.635
12.819
1.00
14.86
C


ATOM
1308
O
GLY
A
93
15.203
27.819
12.473
1.00
17.02
O


ATOM
1309
N
VAL
A
94
16.231
26.278
13.759
1.00
14.32
N


ATOM
1311
CA
VAL
A
94
16.981
27.306
14.421
1.00
14.34
C


ATOM
1313
CB
VAL
A
94
17.654
26.753
15.712
1.00
13.59
C


ATOM
1315
CG1
VAL
A
94
18.263
27.876
16.515
1.00
13.33
C


ATOM
1319
CG2
VAL
A
94
16.633
26.043
16.538
1.00
13.90
C


ATOM
1323
C
VAL
A
94
18.010
28.055
13.577
1.00
14.53
C


ATOM
1324
O
VAL
A
94
18.196
29.258
13.779
1.00
16.71
O


ATOM
1325
N
ALA
A
95
18.724
27.356
12.692
1.00
14.24
N


ATOM
1327
CA
ALA
A
95
19.859
27.839
11.990
1.00
14.00
C


ATOM
1329
CB
ALA
A
95
21.100
27.227
12.574
1.00
14.43
C


ATOM
1333
C
ALA
A
95
19.757
27.491
10.498
1.00
15.52
C


ATOM
1334
O
ALA
A
95
20.476
26.644
9.954
1.00
13.66
O


ATOM
1335
N
PRO
A
96
18.847
28.184
9.840
1.00
15.59
N


ATOM
1336
CA
PRO
A
96
18.487
27.876
8.443
1.00
16.93
C


ATOM
1338
CB
PRO
A
96
17.330
28.851
8.170
1.00
17.23
C


ATOM
1341
CG
PRO
A
96
17.628
29.986
9.086
1.00
16.22
C


ATOM
1344
CD
PRO
A
96
18.078
29.307
10.383
1.00
13.71
C


ATOM
1347
C
PRO
A
96
19.598
28.049
7.403
1.00
17.82
C


ATOM
1348
O
PRO
A
96
19.478
27.477
6.306
1.00
16.77
O


ATOM
1349
N
GLN
A
97
20.664
28.772
7.719
1.00
17.73
N


ATOM
1351
CA
GLN
A
97
21.812
28.891
6.826
1.00
18.02
C


ATOM
1353
CB
GLN
A
97
22.374
30.341
6.726
1.00
18.03
C


ATOM
1356
CG
GLN
A
97
21.509
31.218
5.783
1.00
22.97
C


ATOM
1359
CD
GLN
A
97
20.220
31.715
6.401
1.00
23.17
C


ATOM
1360
OE1
GLN
A
97
20.303
32.467
7.345
1.00
27.41
O


ATOM
1361
NE2
GLN
A
97
19.016
31.311
5.865
1.00
26.08
N


ATOM
1364
C
GLN
A
97
22.901
27.903
7.080
1.00
17.35
C


ATOM
1365
O
GLN
A
97
23.900
27.913
6.351
1.00
17.54
O


ATOM
1366
N
ALA
A
98
22.763
27.057
8.125
1.00
15.56
N


ATOM
1368
CA
ALA
A
98
23.794
26.040
8.361
1.00
16.07
C


ATOM
1370
CB
ALA
A
98
23.615
25.387
9.738
1.00
15.36
C


ATOM
1374
C
ALA
A
98
23.657
24.997
7.256
1.00
15.80
C


ATOM
1375
O
ALA
A
98
22.610
24.906
6.610
1.00
18.34
O


ATOM
1376
N
LYS
A
99
24.683
24.195
7.082
1.00
16.19
N


ATOM
1378
CA
LYS
A
99
24.670
23.108
6.118
1.00
15.45
C


ATOM
1380
CB
LYS
A
99
25.882
23.152
5.268
1.00
16.64
C


ATOM
1383
CG
LYS
A
99
25.789
24.264
4.222
1.00
17.86
C


ATOM
1386
CD
LYS
A
99
24.616
24.101
3.322
1.00
22.94
C


ATOM
1389
CE
LYS
A
99
24.844
25.062
2.185
1.00
28.36
C


ATOM
1392
NZ
LYS
A
99
23.614
25.181
1.383
1.00
28.94
N


ATOM
1396
C
LYS
A
99
24.604
21.759
6.887
1.00
15.54
C


ATOM
1397
O
LYS
A
99
25.012
21.684
8.019
1.00
15.10
O


ATOM
1398
N
LEU
A
100
24.136
20.742
6.185
1.00
14.73
N


ATOM
1400
CA
LEU
A
100
23.801
19.460
6.791
1.00
15.28
C


ATOM
1402
CB
LEU
A
100
22.361
19.131
6.489
1.00
14.80
C


ATOM
1405
CG
LEU
A
100
21.852
17.724
6.719
1.00
16.65
C


ATOM
1407
CD1
LEU
A
100
21.751
17.484
8.242
1.00
17.43
C


ATOM
1411
CD2
LEU
A
100
20.500
17.473
6.155
1.00
17.01
C


ATOM
1415
C
LEU
A
100
24.743
18.373
6.336
1.00
15.64
C


ATOM
1416
O
LEU
A
100
25.114
18.290
5.154
1.00
17.60
O


ATOM
1417
N
TRP
A
101
25.206
17.566
7.298
1.00
15.09
N


ATOM
1419
CA
TRP
A
101
25.895
16.350
6.942
1.00
15.10
C


ATOM
1421
CB
TRP
A
101
27.265
16.234
7.534
1.00
14.66
C


ATOM
1424
CG
TRP
A
101
28.408
17.171
7.076
1.00
13.68
C


ATOM
1425
CD1
TRP
A
101
28.342
18.164
6.137
1.00
14.24
C


ATOM
1427
NE1
TRP
A
101
29.575
18.741
5.956
1.00
14.73
N


ATOM
1429
CE2
TRP
A
101
30.465
18.110
6.770
1.00
14.20
C


ATOM
1430
CD2
TRP
A
101
29.751
17.123
7.498
1.00
15.01
C


ATOM
1431
CE3
TRP
A
101
30.470
16.329
8.413
1.00
15.44
C


ATOM
1433
CZ3
TRP
A
101
31.791
16.598
8.605
1.00
14.31
C


ATOM
1435
CH2
TRP
A
101
32.451
17.587
7.845
1.00
15.17
C


ATOM
1437
CZ2
TRP
A
101
31.780
18.363
6.977
1.00
14.09
C


ATOM
1439
C
TRP
A
101
24.932
15.267
7.451
1.00
16.46
C


ATOM
1440
O
TRP
A
101
24.830
15.022
8.675
1.00
14.81
O


ATOM
1441
N
ALA
A
102
24.250
14.579
6.534
1.00
14.78
N


ATOM
1443
CA
ALA
A
102
23.255
13.610
6.910
1.00
15.54
C


ATOM
1445
CB
ALA
A
102
22.086
13.639
5.973
1.00
16.18
C


ATOM
1449
C
ALA
A
102
23.897
12.221
6.941
1.00
15.83
C


ATOM
1450
O
ALA
A
102
24.187
11.661
5.898
1.00
15.17
O


ATOM
1451
N
TYR
A
103
24.148
11.692
8.140
1.00
14.35
N


ATOM
1453
CA
TYR
A
103
24.797
10.400
8.290
1.00
14.35
C


ATOM
1455
CB
TYR
A
103
25.985
10.493
9.225
1.00
13.92
C


ATOM
1458
CG
TYR
A
103
27.247
11.147
8.697
1.00
13.29
C


ATOM
1459
CD1
TYR
A
103
27.275
11.938
7.550
1.00
14.70
C


ATOM
1461
CE1
TYR
A
103
28.455
12.512
7.113
1.00
14.99
C


ATOM
1463
CZ
TYR
A
103
29.587
12.335
7.783
1.00
13.42
C


ATOM
1464
OH
TYR
A
103
30.820
12.886
7.417
1.00
17.55
O


ATOM
1466
CE2
TYR
A
103
29.608
11.561
8.961
1.00
11.55
C


ATOM
1468
CD2
TYR
A
103
28.445
10.996
9.399
1.00
12.45
C


ATOM
1470
C
TYR
A
103
23.813
9.419
8.860
1.00
13.70
C


ATOM
1471
O
TYR
A
103
23.336
9.583
9.966
1.00
13.01
O


ATOM
1472
N
LYS
A
104
23.490
8.383
8.101
1.00
13.67
N


ATOM
1474
CA
LYS
A
104
22.524
7.385
8.564
1.00
12.46
C


ATOM
1476
CB
LYS
A
104
21.773
6.738
7.407
1.00
14.90
C


ATOM
1479
CG
LYS
A
104
20.789
5.718
7.815
1.00
15.07
C


ATOM
1482
CD
LYS
A
104
19.991
5.144
6.616
1.00
14.19
C


ATOM
1485
CE
LYS
A
104
18.751
4.402
7.036
1.00
17.35
C


ATOM
1488
NZ
LYS
A
104
18.027
3.784
5.831
1.00
15.18
N


ATOM
1492
C
LYS
A
104
23.249
6.327
9.362
1.00
14.29
C


ATOM
1493
O
LYS
A
104
24.138
5.652
8.836
1.00
14.08
O


ATOM
1494
N
VAL
A
105
22.893
6.215
10.645
1.00
12.75
N


ATOM
1496
CA
VAL
A
105
23.513
5.287
11.592
1.00
13.70
C


ATOM
1498
CB
VAL
A
105
24.301
6.043
12.684
1.00
13.12
C


ATOM
1500
CG1
VAL
A
105
25.244
6.961
12.010
1.00
14.29
C


ATOM
1504
CG2
VAL
A
105
23.388
6.804
13.578
1.00
12.47
C


ATOM
1508
C
VAL
A
105
22.491
4.405
12.292
1.00
13.35
C


ATOM
1509
O
VAL
A
105
22.851
3.501
13.036
1.00
14.40
O


ATOM
1510
N
LEU
A
106
21.218
4.733
12.140
1.00
13.80
N


ATOM
1512
CA
LEU
A
106
20.133
3.912
12.678
1.00
15.31
C


ATOM
1514
CB
LEU
A
106
19.165
4.715
13.533
1.00
14.78
C


ATOM
1517
CG
LEU
A
106
19.820
5.395
14.752
1.00
14.84
C


ATOM
1519
CD1
LEU
A
106
18.745
6.216
15.434
1.00
13.44
C


ATOM
1523
CD2
LEU
A
106
20.365
4.281
15.645
1.00
13.80
C


ATOM
1527
C
LEU
A
106
19.328
3.395
11.488
1.00
16.71
C


ATOM
1528
O
LEU
A
106
19.217
4.083
10.457
1.00
14.73
O


ATOM
1529
N
GLY
A
107
18.812
2.184
11.617
1.00
18.94
N


ATOM
1531
CA
GLY
A
107
17.950
1.629
10.581
1.00
20.89
C


ATOM
1534
C
GLY
A
107
16.534
2.176
10.597
1.00
22.18
C


ATOM
1535
O
GLY
A
107
16.136
3.087
11.335
1.00
21.65
O


ATOM
1536
N
ASP
A
108
15.714
1.570
9.755
1.00
24.79
N


ATOM
1538
CA
ASP
A
108
14.419
2.139
9.442
1.00
25.79
C


ATOM
1540
CB
ASP
A
108
13.946
1.584
8.117
1.00
26.09
C


ATOM
1543
CG
ASP
A
108
14.971
1.774
7.022
1.00
28.51
C


ATOM
1544
OD1
ASP
A
108
15.795
2.721
7.082
1.00
27.80
O


ATOM
1545
OD2
ASP
A
108
15.020
1.038
6.025
1.00
30.77
O


ATOM
1546
C
ASP
A
108
13.331
1.997
10.489
1.00
26.41
C


ATOM
1547
O
ASP
A
108
12.229
2.535
10.294
1.00
26.78
O


ATOM
1548
N
ASN
A
109
13.629
1.262
11.566
1.00
26.19
N


ATOM
1550
CA
ASN
A
109
12.751
1.172
12.719
1.00
25.53
C


ATOM
1552
CB
ASN
A
109
12.399
−0.264
13.022
1.00
26.84
C


ATOM
1555
CG
ASN
A
109
11.599
−0.920
11.863
1.00
30.82
C


ATOM
1556
OD1
ASN
A
109
10.606
−0.338
11.353
1.00
35.24
O


ATOM
1557
ND2
ASN
A
109
12.038
−2.093
11.429
1.00
36.51
N


ATOM
1560
C
ASN
A
109
13.340
1.943
13.933
1.00
24.23
C


ATOM
1561
O
ASN
A
109
12.941
1.773
15.071
1.00
23.68
O


ATOM
1562
N
GLY
A
110
14.325
2.774
13.652
1.00
22.01
N


ATOM
1564
CA
GLY
A
110
14.823
3.684
14.685
1.00
20.58
C


ATOM
1567
C
GLY
A
110
15.783
3.001
15.656
1.00
18.79
C


ATOM
1568
O
GLY
A
110
15.989
3.492
16.797
1.00
18.63
O


ATOM
1569
N
SER
A
111
16.373
1.885
15.208
1.00
16.52
N


ATOM
1571
CA
SER
A
111
17.377
1.225
16.057
1.00
17.48
C


ATOM
1573
CB
SER
A
111
16.805
0.002
16.752
1.00
18.93
C


ATOM
1576
OG
SER
A
111
16.663
−1.046
15.856
1.00
20.57
O


ATOM
1578
C
SER
A
111
18.625
0.916
15.250
1.00
15.84
C


ATOM
1579
O
SER
A
111
18.585
0.814
14.022
1.00
15.08
O


ATOM
1580
N
GLY
A
112
19.767
0.761
15.913
1.00
15.46
N


ATOM
1582
CA
GLY
A
112
20.991
0.537
15.198
1.00
14.74
C


ATOM
1585
C
GLY
A
112
22.080
−0.033
16.063
1.00
13.99
C


ATOM
1586
O
GLY
A
112
21.852
−0.365
17.219
1.00
12.44
O


ATOM
1587
N
TYR
A
113
23.229
−0.171
15.431
1.00
13.65
N


ATOM
1589
CA
TYR
A
113
24.372
−0.836
16.000
1.00
14.69
C


ATOM
1591
CB
TYR
A
113
24.992
−1.754
14.982
1.00
14.83
C


ATOM
1594
CG
TYR
A
113
24.139
−2.928
14.627
1.00
16.70
C


ATOM
1595
CD1
TYR
A
113
24.217
−4.098
15.339
1.00
18.38
C


ATOM
1597
CE1
TYR
A
113
23.392
−5.213
14.997
1.00
18.28
C


ATOM
1599
CZ
TYR
A
113
22.516
−5.121
13.926
1.00
19.64
C


ATOM
1600
OH
TYR
A
113
21.730
−6.214
13.573
1.00
20.11
O


ATOM
1602
CE2
TYR
A
113
22.465
−3.958
13.189
1.00
19.66
C


ATOM
1604
CD2
TYR
A
113
23.269
−2.871
13.557
1.00
15.59
C


ATOM
1606
C
TYR
A
113
25.441
0.094
16.471
1.00
13.86
C


ATOM
1607
O
TYR
A
113
25.825
1.019
15.758
1.00
14.60
O


ATOM
1608
N
SER
A
114
25.880
−0.113
17.706
1.00
13.30
N


ATOM
1610
CA
SER
A
114
26.958
0.655
18.290
1.00
13.35
C


ATOM
1612
CB
SER
A
114
27.507
−0.213
19.432
1.00
15.78
C


ATOM
1615
OG
SER
A
114
28.722
0.270
19.995
1.00
14.15
O


ATOM
1617
C
SER
A
114
28.125
0.916
17.339
1.00
14.05
C


ATOM
1618
O
SER
A
114
28.615
2.023
17.226
1.00
13.89
O


ATOM
1619
N
ASP
A
115
28.581
−0.139
16.665
1.00
13.29
N


ATOM
1621
CA
ASP
A
115
29.724
0.014
15.754
1.00
14.72
C


ATOM
1623
CB
AASP
A
115
30.159
−1.299
15.125
0.50
16.85
C


ATOM
1624
CB
BASP
A
115
29.949
−1.288
14.969
0.50
15.71
C


ATOM
1629
CG
AASP
A
115
29.100
−1.900
14.308
0.50
20.25
C


ATOM
1630
CG
BASP
A
115
30.701
−2.324
15.752
0.50
16.91
C


ATOM
1631
OD1
AASP
A
115
28.555
−1.177
13.460
0.50
32.11
O


ATOM
1632
OD1
BASP
A
115
31.693
−1.951
16.379
0.50
24.07
O


ATOM
1633
OD2
AASP
A
115
28.717
−3.057
14.477
0.50
33.25
O


ATOM
1634
OD2
BASP
A
115
30.401
−3.501
15.764
0.50
20.69
O


ATOM
1635
C
ASP
A
115
29.511
1.034
14.682
1.00
13.31
C


ATOM
1636
O
ASP
A
115
30.488
1.671
14.268
1.00
13.00
O


ATOM
1637
N
ASP
A
116
28.292
1.098
14.156
1.00
12.95
N


ATOM
1639
CA
ASP
A
116
27.982
1.996
13.046
1.00
13.15
C


ATOM
1641
CB
ASP
A
116
26.618
1.685
12.446
1.00
14.36
C


ATOM
1644
CG
ASP
A
116
26.506
0.299
11.819
1.00
14.39
C


ATOM
1645
OD1
ASP
A
116
27.509
−0.378
11.592
1.00
13.15
O


ATOM
1646
OD2
ASP
A
116
25.408
−0.179
11.584
1.00
13.28
O


ATOM
1647
C
ASP
A
116
28.001
3.437
13.581
1.00
13.01
C


ATOM
1648
O
ASP
A
116
28.529
4.354
12.937
1.00
13.99
O


ATOM
1649
N
ILE
A
117
27.409
3.644
14.748
1.00
13.05
N


ATOM
1651
CA
ILE
A
117
27.404
4.952
15.339
1.00
12.04
C


ATOM
1653
CB
ILE
A
117
26.518
4.961
16.572
1.00
12.89
C


ATOM
1655
CG1
ILE
A
117
25.034
4.744
16.168
1.00
17.41
C


ATOM
1658
CD1
ILE
A
117
24.279
3.948
17.085
1.00
21.55
C


ATOM
1662
CG2
ILE
A
117
26.715
6.288
17.378
1.00
15.14
C


ATOM
1666
C
ILE
A
117
28.813
5.403
15.623
1.00
11.86
C


ATOM
1667
O
ILE
A
117
29.195
6.548
15.321
1.00
12.61
O


ATOM
1668
N
ALA
A
118
29.609
4.532
16.227
1.00
10.45
N


ATOM
1670
CA
ALA
A
118
30.981
4.891
16.519
1.00
11.56
C


ATOM
1672
CB
ALA
A
118
31.649
3.800
17.353
1.00
11.97
C


ATOM
1676
C
ALA
A
118
31.786
5.248
15.273
1.00
11.35
C


ATOM
1677
O
ALA
A
118
32.511
6.241
15.232
1.00
10.56
O


ATOM
1678
N
ALA
A
119
31.597
4.459
14.253
1.00
11.09
N


ATOM
1680
CA
ALA
A
119
32.298
4.693
13.010
1.00
10.91
C


ATOM
1682
CB
ALA
A
119
32.030
3.600
12.104
1.00
11.91
C


ATOM
1686
C
ALA
A
119
31.875
6.029
12.430
1.00
10.50
C


ATOM
1687
O
ALA
A
119
32.721
6.808
11.942
1.00
12.46
O


ATOM
1688
N
ALA
A
120
30.589
6.342
12.539
1.00
11.95
N


ATOM
1690
CA
ALA
A
120
30.079
7.579
12.001
1.00
11.18
C


ATOM
1692
CB
ALA
A
120
28.626
7.575
12.034
1.00
11.63
C


ATOM
1696
C
ALA
A
120
30.643
8.813
12.743
1.00
11.63
C


ATOM
1697
O
ALA
A
120
31.033
9.799
12.104
1.00
11.85
O


ATOM
1698
N
ILE
A
121
30.708
8.753
14.070
1.00
10.33
N


ATOM
1700
CA
ILE
A
121
31.291
9.848
14.892
1.00
10.58
C


ATOM
1702
CB
ILE
A
121
31.215
9.481
16.379
1.00
11.12
C


ATOM
1704
CG1
ILE
A
121
29.768
9.320
16.750
1.00
11.01
C


ATOM
1707
CD1
ILE
A
121
29.566
8.668
18.128
1.00
11.36
C


ATOM
1711
CG2
ILE
A
121
31.860
10.543
17.229
1.00
12.83
C


ATOM
1715
C
ILE
A
121
32.749
10.129
14.510
1.00
11.68
C


ATOM
1716
O
ILE
A
121
33.158
11.259
14.287
1.00
12.00
O


ATOM
1717
N
ARG
A
122
33.536
9.055
14.448
1.00
11.70
N


ATOM
1719
CA
ARG
A
122
34.929
9.171
14.046
1.00
13.86
C


ATOM
1721
CB
ARG
A
122
35.603
7.810
14.114
1.00
14.57
C


ATOM
1724
CG
ARG
A
122
35.715
7.320
15.531
1.00
14.77
C


ATOM
1727
CD
ARG
A
122
36.384
5.975
15.679
1.00
18.79
C


ATOM
1730
NE
ARG
A
122
36.784
5.757
17.048
1.00
21.92
N


ATOM
1732
CZ
ARG
A
122
37.945
6.112
17.577
1.00
22.62
C


ATOM
1733
NH1
ARG
A
122
38.894
6.640
16.838
1.00
20.73
N


ATOM
1736
NH2
ARG
A
122
38.178
5.850
18.857
1.00
29.11
N


ATOM
1739
C
ARG
A
122
35.088
9.760
12.636
1.00
14.45
C


ATOM
1740
O
ARG
A
122
35.992
10.563
12.389
1.00
13.33
O


ATOM
1741
N
HIS
A
123
34.198
9.348
11.743
1.00
12.96
N


ATOM
1743
CA
HIS
A
123
34.231
9.828
10.385
1.00
14.40
C


ATOM
1745
CB
HIS
A
123
33.324
9.001
9.522
1.00
14.46
C


ATOM
1748
CG
HIS
A
123
33.390
9.347
8.065
1.00
17.57
C


ATOM
1749
ND1
HIS
A
123
34.358
8.843
7.224
1.00
26.34
N


ATOM
1751
CE1
HIS
A
123
34.183
9.333
6.005
1.00
26.65
C


ATOM
1753
NE2
HIS
A
123
33.120
10.115
6.015
1.00
23.02
N


ATOM
1755
CD2
HIS
A
123
32.596
10.125
7.299
1.00
22.31
C


ATOM
1757
C
HIS
A
123
33.913
11.345
10.332
1.00
15.58
C


ATOM
1758
O
HIS
A
123
34.587
12.095
9.658
1.00
14.13
O


ATOM
1759
N
VAL
A
124
32.914
11.801
11.081
1.00
13.58
N


ATOM
1761
CA
VAL
A
124
32.701
13.233
11.195
1.00
13.94
C


ATOM
1763
CB
VAL
A
124
31.583
13.598
12.235
1.00
13.67
C


ATOM
1765
CG1
VAL
A
124
31.476
15.111
12.408
1.00
11.98
C


ATOM
1769
CG2
VAL
A
124
30.258
13.019
11.847
1.00
14.52
C


ATOM
1773
C
VAL
A
124
33.980
13.971
11.580
1.00
12.11
C


ATOM
1774
O
VAL
A
124
34.323
14.973
10.938
1.00
13.19
O


ATOM
1775
N
ALA
A
125
34.672
13.495
12.604
1.00
12.61
N


ATOM
1777
CA
ALA
A
125
35.875
14.123
13.121
1.00
12.03
C


ATOM
1779
CB
ALA
A
125
36.351
13.398
14.322
1.00
11.98
C


ATOM
1783
C
ALA
A
125
36.972
14.158
12.062
1.00
13.52
C


ATOM
1784
O
ALA
A
125
37.610
15.186
11.838
1.00
13.20
O


ATOM
1785
N
ASP
A
126
37.081
13.059
11.312
1.00
13.79
N


ATOM
1787
CA
ASP
A
126
38.087
12.980
10.268
1.00
15.94
C


ATOM
1789
CB
ASP
A
126
38.180
11.566
9.743
1.00
16.11
C


ATOM
1792
CG
ASP
A
126
38.895
10.635
10.677
1.00
16.70
C


ATOM
1793
OD1
ASP
A
126
39.620
11.075
11.580
1.00
17.85
O


ATOM
1794
OD2
ASP
A
126
38.795
9.393
10.586
1.00
17.09
O


ATOM
1795
C
ASP
A
126
37.736
13.933
9.133
1.00
16.71
C


ATOM
1796
O
ASP
A
126
38.604
14.612
8.602
1.00
17.22
O


ATOM
1797
N
GLU
A
127
36.465
14.027
8.798
1.00
16.48
N


ATOM
1799
CA
GLU
A
127
36.033
14.934
7.759
1.00
17.08
C


ATOM
1801
CB
GLU
A
127
34.580
14.723
7.386
1.00
17.14
C


ATOM
1804
CG
GLU
A
127
34.319
13.431
6.618
1.00
18.23
C


ATOM
1807
CD
GLU
A
127
34.875
13.485
5.205
1.00
23.17
C


ATOM
1808
OE1
GLU
A
127
34.333
14.250
4.412
1.00
22.57
O


ATOM
1809
OE2
GLU
A
127
35.887
12.809
4.967
1.00
25.80
O


ATOM
1810
C
GLU
A
127
36.256
16.370
8.204
1.00
18.24
C


ATOM
1811
O
GLU
A
127
36.634
17.255
7.393
1.00
17.10
O


ATOM
1812
N
ALA
A
128
35.969
16.626
9.465
1.00
16.20
N


ATOM
1814
CA
ALA
A
128
36.165
17.979
10.000
1.00
17.01
C


ATOM
1816
CB
ALA
A
128
35.582
18.100
11.469
1.00
18.11
C


ATOM
1820
C
ALA
A
128
37.607
18.403
9.959
1.00
17.06
C


ATOM
1821
O
ALA
A
128
37.923
19.537
9.561
1.00
18.46
O


ATOM
1822
N
SER
A
129
38.496
17.519
10.360
1.00
17.73
N


ATOM
1824
CA
SER
A
129
39.896
17.869
10.334
1.00
19.04
C


ATOM
1826
CB
SER
A
129
40.735
16.796
10.996
1.00
19.88
C


ATOM
1829
OG
SER
A
129
40.289
15.493
10.649
1.00
28.54
O


ATOM
1831
C
SER
A
129
40.367
18.063
8.907
1.00
18.31
C


ATOM
1832
O
SER
A
129
41.158
18.968
8.641
1.00
19.92
O


ATOM
1833
N
ARG
A
130
39.927
17.209
8.003
1.00
17.50
N


ATOM
1835
CA
ARG
A
130
40.418
17.258
6.611
1.00
17.79
C


ATOM
1837
CB
ARG
A
130
39.938
16.052
5.802
1.00
17.78
C


ATOM
1840
CG
ARG
A
130
40.573
15.989
4.406
1.00
17.70
C


ATOM
1843
CD
ARG
A
130
40.048
14.864
3.632
1.00
18.82
C


ATOM
1846
NE
ARG
A
130
38.768
15.271
3.064
1.00
25.15
N


ATOM
1848
CZ
ARG
A
130
37.641
14.760
3.393
1.00
25.02
C


ATOM
1849
NH1
ARG
A
130
37.620
13.808
4.337
1.00
26.77
N


ATOM
1852
NH2
ARG
A
130
36.524
15.209
2.775
1.00
23.08
N


ATOM
1855
C
ARG
A
130
39.990
18.543
5.958
1.00
19.20
C


ATOM
1856
O
ARG
A
130
40.816
19.255
5.352
1.00
20.50
O


ATOM
1857
N
THR
A
131
38.740
18.915
6.155
1.00
18.72
N


ATOM
1859
CA
THR
A
131
38.127
20.089
5.461
1.00
19.43
C


ATOM
1861
CB
THR
A
131
36.673
19.866
5.244
1.00
20.43
C


ATOM
1863
OG1
THR
A
131
35.973
19.754
6.517
1.00
18.62
O


ATOM
1865
CG2
THR
A
131
36.421
18.548
4.449
1.00
22.94
C


ATOM
1869
C
THR
A
131
38.262
21.415
6.203
1.00
20.40
C


ATOM
1870
O
THR
A
131
37.906
22.461
5.657
1.00
20.95
O


ATOM
1871
N
GLY
A
132
38.758
21.356
7.431
1.00
18.57
N


ATOM
1873
CA
GLY
A
132
38.841
22.513
8.289
1.00
19.51
C


ATOM
1876
C
GLY
A
132
37.464
23.129
8.581
1.00
20.63
C


ATOM
1877
O
GLY
A
132
37.313
24.336
8.829
1.00
23.12
O


ATOM
1878
N
SER
A
133
36.442
22.287
8.646
1.00
18.55
N


ATOM
1880
CA
SER
A
133
35.094
22.754
8.904
1.00
18.11
C


ATOM
1882
CB
SER
A
133
34.080
21.819
8.260
1.00
18.42
C


ATOM
1885
OG
SER
A
133
34.242
21.666
6.844
1.00
21.03
O


ATOM
1887
C
SER
A
133
34.768
22.836
10.427
1.00
16.26
C


ATOM
1888
O
SER
A
133
35.348
22.145
11.284
1.00
15.54
O


ATOM
1889
N
LYS
A
134
33.798
23.687
10.720
1.00
16.27
N


ATOM
1891
CA
LYS
A
134
33.275
23.830
12.096
1.00
15.54
C


ATOM
1893
CB
LYS
A
134
32.921
25.274
12.419
1.00
15.50
C


ATOM
1896
CG
LYS
A
134
34.154
26.176
12.525
1.00
18.18
C


ATOM
1899
CD
LYS
A
134
33.819
27.647
12.502
1.00
25.94
C


ATOM
1902
CE
LYS
A
134
35.064
28.567
12.191
1.00
30.57
C


ATOM
1905
NZ
LYS
A
134
36.391
28.051
12.564
1.00
34.14
N


ATOM
1909
C
LYS
A
134
32.032
22.951
12.094
1.00
13.21
C


ATOM
1910
O
LYS
A
134
31.121
23.177
11.349
1.00
14.18
O


ATOM
1911
N
VAL
A
135
32.015
21.919
12.921
1.00
12.06
N


ATOM
1913
CA
VAL
A
135
30.964
20.957
12.863
1.00
12.02
C


ATOM
1915
CB
VAL
A
135
31.487
19.632
12.321
1.00
13.28
C


ATOM
1917
CG1
VAL
A
135
30.363
18.596
12.114
1.00
14.27
C


ATOM
1921
CG2
VAL
A
135
32.322
19.867
11.006
1.00
14.42
C


ATOM
1925
C
VAL
A
135
30.383
20.673
14.241
1.00
11.74
C


ATOM
1926
O
VAL
A
135
31.097
20.566
15.220
1.00
11.76
O


ATOM
1927
N
VAL
A
136
29.071
20.604
14.286
1.00
11.28
N


ATOM
1929
CA
VAL
A
136
28.389
20.206
15.478
1.00
10.73
C


ATOM
1931
CB
VAL
A
136
27.285
21.177
15.819
1.00
11.25
C


ATOM
1933
CG1
VAL
A
136
26.576
20.755
17.127
1.00
13.78
C


ATOM
1937
CG2
VAL
A
136
27.897
22.594
16.013
1.00
12.65
C


ATOM
1941
C
VAL
A
136
27.702
18.852
15.159
1.00
11.22
C


ATOM
1942
O
VAL
A
136
26.973
18.747
14.178
1.00
11.53
O


ATOM
1943
N
ILE
A
137
27.928
17.850
15.993
1.00
9.65
N


ATOM
1945
CA
ILE
A
137
27.255
16.573
15.854
1.00
10.61
C


ATOM
1947
CB
ILE
A
137
28.113
15.459
16.381
1.00
9.23
C


ATOM
1949
CG1
ILE
A
137
29.215
15.121
15.395
1.00
11.83
C


ATOM
1952
CD1
ILE
A
137
30.266
14.104
15.930
1.00
11.56
C


ATOM
1956
CG2
ILE
A
137
27.238
14.230
16.611
1.00
10.38
C


ATOM
1960
C
ILE
A
137
25.993
16.610
16.690
1.00
10.44
C


ATOM
1961
O
ILE
A
137
26.031
17.014
17.869
1.00
11.92
O


ATOM
1962
N
ASN
A
138
24.899
16.203
16.096
1.00
9.00
N


ATOM
1964
CA
ASN
A
138
23.654
15.942
16.764
1.00
10.52
C


ATOM
1966
CB
ASN
A
138
22.494
16.601
15.996
1.00
8.55
C


ATOM
1969
CG
ASN
A
138
21.146
16.503
16.715
1.00
12.38
C


ATOM
1970
OD1
ASN
A
138
20.648
17.515
17.236
1.00
10.74
O


ATOM
1971
ND2
ASN
A
138
20.519
15.297
16.722
1.00
9.55
N


ATOM
1974
C
ASN
A
138
23.376
14.477
16.861
1.00
10.74
C


ATOM
1975
O
ASN
A
138
23.256
13.799
15.833
1.00
9.80
O


ATOM
1976
N
MET
A
139
23.208
13.987
18.091
1.00
9.89
N


ATOM
1978
CA
MET
A
139
22.830
12.585
18.304
1.00
10.28
C


ATOM
1980
CB
MET
A
139
23.975
11.764
18.906
1.00
9.62
C


ATOM
1983
CG
MET
A
139
24.984
11.345
17.895
1.00
8.67
C


ATOM
1986
SD
MET
A
139
26.240
10.206
18.525
1.00
11.34
S


ATOM
1987
CE
MET
A
139
27.161
11.324
19.556
1.00
13.56
C


ATOM
1991
C
MET
A
139
21.581
12.474
19.162
1.00
10.20
C


ATOM
1992
O
MET
A
139
21.587
12.497
20.413
1.00
9.22
O


ATOM
1993
N
SER
A
140
20.467
12.424
18.458
1.00
10.57
N


ATOM
1995
CA
SER
A
140
19.166
12.207
19.083
1.00
10.10
C


ATOM
1997
CB
SER
A
140
18.082
12.817
18.201
1.00
10.22
C


ATOM
2000
OG
SER
A
140
18.142
14.229
18.264
1.00
11.42
O


ATOM
2002
C
SER
A
140
18.959
10.705
19.255
1.00
11.04
C


ATOM
2003
O
SER
A
140
18.006
10.119
18.716
1.00
10.43
O


ATOM
2004
N
LEU
A
141
19.844
10.063
20.011
1.00
10.62
N


ATOM
2006
CA
LEU
A
141
19.890
8.627
20.116
1.00
10.50
C


ATOM
2008
CB
LEU
A
141
20.446
8.014
18.833
1.00
10.74
C


ATOM
2011
CG
LEU
A
141
21.881
8.483
18.439
1.00
11.49
C


ATOM
2013
CD1
LEU
A
141
22.939
7.897
19.316
1.00
11.20
C


ATOM
2017
CD2
LEU
A
141
22.119
8.057
17.001
1.00
12.54
C


ATOM
2021
C
LEU
A
141
20.748
8.223
21.297
1.00
9.69
C


ATOM
2022
O
LEU
A
141
21.402
9.060
21.892
1.00
9.38
O


ATOM
2023
N
GLY
A
142
20.640
6.975
21.689
1.00
11.48
N


ATOM
2025
CA
GLY
A
142
21.450
6.472
22.789
1.00
10.69
C


ATOM
2028
C
GLY
A
142
21.046
5.146
23.335
1.00
9.74
C


ATOM
2029
O
GLY
A
142
20.283
4.413
22.660
1.00
12.20
O


ATOM
2030
N
SER
A
143
21.517
4.850
24.562
1.00
11.68
N


ATOM
2032
CA
SER
A
143
21.200
3.610
25.308
1.00
11.10
C


ATOM
2034
CB
SER
A
143
22.187
2.489
25.050
1.00
11.52
C


ATOM
2037
OG
SER
A
143
23.517
2.918
25.316
1.00
11.14
O


ATOM
2039
C
SER
A
143
21.272
3.995
26.777
1.00
12.09
C


ATOM
2040
O
SER
A
143
21.941
4.966
27.161
1.00
11.62
O


ATOM
2041
N
SER
A
144
20.523
3.292
27.587
1.00
11.90
N


ATOM
2043
CA
SER
A
144
20.527
3.552
28.992
1.00
11.92
C


ATOM
2045
CB
SER
A
144
19.513
2.623
29.653
1.00
13.61
C


ATOM
2048
OG
SER
A
144
19.521
2.848
31.017
1.00
18.36
O


ATOM
2050
C
SER
A
144
21.903
3.362
29.593
1.00
13.78
C


ATOM
2051
O
SER
A
144
22.341
4.161
30.401
1.00
14.05
O


ATOM
2052
N
ALA
A
145
22.551
2.278
29.189
1.00
12.68
N


ATOM
2054
CA
ALA
A
145
23.892
1.972
29.678
1.00
11.54
C


ATOM
2056
CB
ALA
A
145
24.135
0.503
29.488
1.00
12.43
C


ATOM
2060
C
ALA
A
145
24.956
2.745
28.886
1.00
12.26
C


ATOM
2061
O
ALA
A
145
24.814
3.001
27.712
1.00
12.18
O


ATOM
2062
N
LYS
A
146
26.067
3.066
29.521
1.00
10.71
N


ATOM
2064
CA
LYS
A
146
27.184
3.640
28.760
1.00
11.08
C


ATOM
2066
CB
LYS
A
146
28.219
4.095
29.754
1.00
10.62
C


ATOM
2069
CG
LYS
A
146
29.563
4.535
29.197
1.00
13.37
C


ATOM
2072
CD
LYS
A
146
30.506
5.206
30.245
1.00
14.55
C


ATOM
2075
CE
LYS
A
146
31.796
5.638
29.643
1.00
17.59
C


ATOM
2078
NZ
LYS
A
146
32.732
6.238
30.665
1.00
17.20
N


ATOM
2082
C
LYS
A
146
27.767
2.559
27.834
1.00
8.93
C


ATOM
2083
O
LYS
A
146
27.978
1.383
28.241
1.00
11.42
O


ATOM
2084
N
ASP
A
147
28.075
2.956
26.621
1.00
10.15
N


ATOM
2086
CA
ASP
A
147
28.694
2.129
25.575
1.00
10.08
C


ATOM
2088
CB
ASP
A
147
27.843
2.189
24.296
1.00
10.84
C


ATOM
2091
CG
ASP
A
147
28.460
1.509
23.117
1.00
11.61
C


ATOM
2092
OD1
ASP
A
147
29.701
1.630
22.895
1.00
11.69
O


ATOM
2093
OD2
ASP
A
147
27.753
0.879
22.305
1.00
10.96
O


ATOM
2094
C
ASP
A
147
30.057
2.784
25.353
1.00
10.79
C


ATOM
2095
O
ASP
A
147
30.163
3.902
24.859
1.00
9.34
O


ATOM
2096
N
SER
A
148
31.104
2.055
25.717
1.00
9.95
N


ATOM
2098
CA
SER
A
148
32.450
2.588
25.641
1.00
11.43
C


ATOM
2100
CB
SER
A
148
33.431
1.838
26.533
1.00
11.86
C


ATOM
2103
OG
SER
A
148
33.164
2.051
27.904
1.00
13.07
O


ATOM
2105
C
SER
A
148
32.999
2.765
24.221
1.00
12.17
C


ATOM
2106
O
SER
A
148
33.958
3.537
24.021
1.00
10.72
O


ATOM
2107
N
LEU
A
149
32.471
1.997
23.269
1.00
9.59
N


ATOM
2109
CA
LEU
A
149
32.936
2.120
21.909
1.00
10.95
C


ATOM
2111
CB
LEU
A
149
32.374
1.029
21.030
1.00
11.13
C


ATOM
2114
CG
LEU
A
149
32.863
1.087
19.569
1.00
13.82
C


ATOM
2116
CD1
LEU
A
149
34.404
0.961
19.536
1.00
14.72
C


ATOM
2120
CD2
LEU
A
149
32.162
0.036
18.699
1.00
15.05
C


ATOM
2124
C
LEU
A
149
32.474
3.511
21.419
1.00
10.85
C


ATOM
2125
O
LEU
A
149
33.201
4.268
20.807
1.00
10.58
O


ATOM
2126
N
ILE
A
150
31.223
3.823
21.649
1.00
9.24
N


ATOM
2128
CA
ILE
A
150
30.671
5.127
21.255
1.00
9.06
C


ATOM
2130
CB
ILE
A
150
29.145
5.136
21.471
1.00
8.95
C


ATOM
2132
CG1
ILE
A
150
28.499
4.376
20.337
1.00
9.94
C


ATOM
2135
CD1
ILE
A
150
27.038
4.154
20.508
1.00
11.53
C


ATOM
2139
CG2
ILE
A
150
28.601
6.553
21.538
1.00
11.45
C


ATOM
2143
C
ILE
A
150
31.433
6.226
22.052
1.00
7.79
C


ATOM
2144
O
ILE
A
150
31.793
7.297
21.500
1.00
8.15
O


ATOM
2145
N
ALA
A
151
31.724
5.956
23.300
1.00
8.34
N


ATOM
2147
CA
ALA
A
151
32.396
6.930
24.152
1.00
9.35
C


ATOM
2149
CB
ALA
A
151
32.479
6.402
25.577
1.00
10.22
C


ATOM
2153
C
ALA
A
151
33.796
7.240
23.629
1.00
9.49
C


ATOM
2154
O
ALA
A
151
34.215
8.422
23.583
1.00
9.45
O


ATOM
2155
N
SER
A
152
34.508
6.177
23.181
1.00
10.40
N


ATOM
2157
CA
SER
A
152
35.823
6.371
22.613
1.00
11.13
C


ATOM
2159
CB
SER
A
152
36.466
5.047
22.278
1.00
10.46
C


ATOM
2162
OG
SER
A
152
37.628
5.216
21.460
1.00
13.86
O


ATOM
2164
C
SER
A
152
35.737
7.285
21.349
1.00
11.17
C


ATOM
2165
O
SER
A
152
36.585
8.207
21.144
1.00
11.47
O


ATOM
2166
N
ALA
A
153
34.688
7.103
20.578
1.00
11.26
N


ATOM
2168
CA
ALA
A
153
34.476
7.917
19.358
1.00
11.23
C


ATOM
2170
CB
ALA
A
153
33.413
7.295
18.527
1.00
12.05
C


ATOM
2174
C
ALA
A
153
34.143
9.349
19.699
1.00
11.53
C


ATOM
2175
O
ALA
A
153
34.699
10.314
19.103
1.00
11.12
O


ATOM
2176
N
VAL
A
154
33.285
9.529
20.695
1.00
9.67
N


ATOM
2178
CA
VAL
A
154
32.941
10.878
21.104
1.00
9.77
C


ATOM
2180
CB
VAL
A
154
31.908
10.810
22.223
1.00
10.26
C


ATOM
2182
CG1
VAL
A
154
31.833
12.136
22.988
1.00
12.19
C


ATOM
2186
CG2
VAL
A
154
30.583
10.402
21.661
1.00
11.04
C


ATOM
2190
C
VAL
A
154
34.229
11.606
21.565
1.00
11.02
C


ATOM
2191
O
VAL
A
154
34.449
12.779
21.212
1.00
11.61
O


ATOM
2192
N
ASP
A
155
35.069
10.954
22.367
1.00
10.47
N


ATOM
2194
CA
ASP
A
155
36.309
11.603
22.838
1.00
12.38
C


ATOM
2196
CB
ASP
A
155
37.040
10.744
23.859
1.00
13.32
C


ATOM
2199
CG
ASP
A
155
36.328
10.668
25.183
1.00
19.66
C


ATOM
2200
OD1
ASP
A
155
35.449
11.508
25.455
1.00
21.98
O


ATOM
2201
OD2
ASP
A
155
36.604
9.813
26.030
1.00
21.73
O


ATOM
2202
C
ASP
A
155
37.242
11.932
21.674
1.00
13.46
C


ATOM
2203
O
ASP
A
155
37.928
12.926
21.695
1.00
12.65
O


ATOM
2204
N
TYR
A
156
37.308
11.034
20.694
1.00
12.30
N


ATOM
2206
CA
TYR
A
156
38.119
11.263
19.510
1.00
11.91
C


ATOM
2208
CB
TYR
A
156
37.992
10.073
18.607
1.00
12.85
C


ATOM
2211
CG
TYR
A
156
38.753
10.140
17.309
1.00
13.57
C


ATOM
2212
CD1
TYR
A
156
40.154
9.965
17.286
1.00
16.30
C


ATOM
2214
CE1
TYR
A
156
40.822
9.989
16.093
1.00
15.71
C


ATOM
2216
CZ
TYR
A
156
40.136
10.172
14.899
1.00
20.85
C


ATOM
2217
OH
TYR
A
156
40.795
10.142
13.677
1.00
19.47
O


ATOM
2219
CE2
TYR
A
156
38.771
10.352
14.883
1.00
14.75
C


ATOM
2221
CD2
TYR
A
156
38.096
10.349
16.111
1.00
14.20
C


ATOM
2223
C
TYR
A
156
37.653
12.497
18.764
1.00
11.70
C


ATOM
2224
O
TYR
A
156
38.463
13.343
18.408
1.00
12.40
O


ATOM
2225
N
ALA
A
157
36.332
12.649
18.630
1.00
11.59
N


ATOM
2227
CA
ALA
A
157
35.773
13.776
17.895
1.00
10.92
C


ATOM
2229
CB
ALA
A
157
34.319
13.493
17.547
1.00
11.98
C


ATOM
2233
C
ALA
A
157
35.926
15.058
18.670
1.00
11.69
C


ATOM
2234
O
ALA
A
157
36.214
16.117
18.072
1.00
11.27
O


ATOM
2235
N
TYR
A
158
35.740
14.996
19.983
1.00
11.31
N


ATOM
2237
CA
TYR
A
158
35.855
16.210
20.809
1.00
12.56
C


ATOM
2239
CB
TYR
A
158
35.410
15.940
22.243
1.00
12.28
C


ATOM
2242
CG
TYR
A
158
35.147
17.188
23.090
1.00
9.96
C


ATOM
2243
CD1
TYR
A
158
34.015
17.937
22.878
1.00
11.56
C


ATOM
2245
CE1
TYR
A
158
33.754
19.051
23.629
1.00
13.20
C


ATOM
2247
CZ
TYR
A
158
34.635
19.477
24.580
1.00
13.62
C


ATOM
2248
OH
TYR
A
158
34.370
20.612
25.295
1.00
12.22
O


ATOM
2250
CE2
TYR
A
158
35.813
18.809
24.787
1.00
13.48
C


ATOM
2252
CD2
TYR
A
158
36.078
17.647
24.028
1.00
12.56
C


ATOM
2254
C
TYR
A
158
37.308
16.655
20.783
1.00
12.48
C


ATOM
2255
O
TYR
A
158
37.591
17.853
20.822
1.00
12.68
O


ATOM
2256
N
GLY
A
159
38.207
15.683
20.642
1.00
12.38
N


ATOM
2258
CA
GLY
A
159
39.628
15.978
20.651
1.00
13.17
C


ATOM
2261
C
GLY
A
159
40.055
16.611
19.371
1.00
13.46
C


ATOM
2262
O
GLY
A
159
41.161
17.165
19.297
1.00
14.28
O


ATOM
2263
N
LYS
A
160
39.238
16.495
18.350
1.00
12.48
N


ATOM
2265
CA
LYS
A
160
39.486
17.099
17.035
1.00
14.66
C


ATOM
2267
CB
LYS
A
160
39.324
16.046
15.953
1.00
15.36
C


ATOM
2270
CG
LYS
A
160
40.421
14.964
15.992
1.00
20.16
C


ATOM
2273
CD
LYS
A
160
40.057
13.848
15.058
1.00
25.00
C


ATOM
2276
CE
LYS
A
160
41.183
13.408
14.161
1.00
31.38
C


ATOM
2279
NZ
LYS
A
160
41.602
14.404
13.204
1.00
30.54
N


ATOM
2283
C
LYS
A
160
38.603
18.344
16.761
1.00
14.03
C


ATOM
2284
O
LYS
A
160
38.469
18.786
15.621
1.00
12.37
O


ATOM
2285
N
GLY
A
161
38.076
18.954
17.829
1.00
13.97
N


ATOM
2287
CA
GLY
A
161
37.363
20.230
17.751
1.00
13.11
C


ATOM
2290
C
GLY
A
161
35.928
20.180
17.281
1.00
12.99
C


ATOM
2291
O
GLY
A
161
35.434
21.185
16.743
1.00
14.25
O


ATOM
2292
N
VAL
A
162
35.269
19.020
17.395
1.00
11.44
N


ATOM
2294
CA
VAL
A
162
33.858
18.848
16.972
1.00
10.32
C


ATOM
2296
CB
VAL
A
162
33.621
17.492
16.309
1.00
11.03
C


ATOM
2298
CG1
VAL
A
162
32.146
17.268
15.950
1.00
11.59
C


ATOM
2302
CG2
VAL
A
162
34.438
17.378
15.034
1.00
13.60
C


ATOM
2306
C
VAL
A
162
32.991
18.918
18.219
1.00
10.43
C


ATOM
2307
O
VAL
A
162
33.306
18.259
19.222
1.00
11.54
O


ATOM
2308
N
LEU
A
163
31.965
19.748
18.217
1.00
9.11
N


ATOM
2310
CA
LEU
A
163
31.075
19.817
19.382
1.00
10.40
C


ATOM
2312
CB
LEU
A
163
30.278
21.105
19.344
1.00
11.53
C


ATOM
2315
CG
LEU
A
163
29.336
21.334
20.515
1.00
10.22
C


ATOM
2317
CD1
LEU
A
163
30.163
21.561
21.748
1.00
12.89
C


ATOM
2321
CD2
LEU
A
163
28.486
22.497
20.248
1.00
13.67
C


ATOM
2325
C
LEU
A
163
30.118
18.647
19.257
1.00
10.89
C


ATOM
2326
O
LEU
A
163
29.620
18.367
18.176
1.00
13.34
O


ATOM
2327
N
ILE
A
164
29.832
17.975
20.347
1.00
11.01
N


ATOM
2329
CA
ILE
A
164
28.860
16.890
20.383
1.00
9.92
C


ATOM
2331
CB
ILE
A
164
29.500
15.573
20.908
1.00
10.24
C


ATOM
2333
CG1
ILE
A
164
30.616
15.070
19.976
1.00
13.39
C


ATOM
2336
CD1
ILE
A
164
31.893
15.389
20.491
1.00
16.69
C


ATOM
2340
CG2
ILE
A
164
28.496
14.458
20.907
1.00
12.88
C


ATOM
2344
C
ILE
A
164
27.673
17.259
21.275
1.00
10.29
C


ATOM
2345
O
ILE
A
164
27.851
17.505
22.479
1.00
10.17
O


ATOM
2346
N
VAL
A
165
26.489
17.191
20.694
1.00
9.19
N


ATOM
2348
CA
VAL
A
165
25.257
17.479
21.391
1.00
9.03
C


ATOM
2350
CB
VAL
A
165
24.576
18.708
20.753
1.00
10.21
C


ATOM
2352
CG1
VAL
A
165
23.300
19.053
21.542
1.00
9.33
C


ATOM
2356
CG2
VAL
A
165
25.483
19.888
20.715
1.00
11.01
C


ATOM
2360
C
VAL
A
165
24.360
16.245
21.311
1.00
8.47
C


ATOM
2361
O
VAL
A
165
24.193
15.681
20.222
1.00
10.40
O


ATOM
2362
N
ALA
A
166
23.833
15.747
22.452
1.00
9.28
N


ATOM
2364
CA
ALA
A
166
23.163
14.469
22.484
1.00
8.57
C


ATOM
2366
CB
ALA
A
166
24.104
13.327
22.794
1.00
9.70
C


ATOM
2370
C
ALA
A
166
22.011
14.454
23.489
1.00
9.40
C


ATOM
2371
O
ALA
A
166
22.028
15.186
24.476
1.00
9.37
O


ATOM
2372
N
ALA
A
167
21.000
13.646
23.186
1.00
10.21
N


ATOM
2374
CA
ALA
A
167
19.794
13.637
23.965
1.00
10.10
C


ATOM
2376
CB
ALA
A
167
18.747
12.726
23.251
1.00
11.66
C


ATOM
2380
C
ALA
A
167
20.086
13.087
25.329
1.00
10.38
C


ATOM
2381
O
ALA
A
167
20.787
12.038
25.431
1.00
8.93
O


ATOM
2382
N
ALA
A
168
19.424
13.572
26.360
1.00
10.36
N


ATOM
2384
CA
ALA
A
168
19.623
13.036
27.685
1.00
10.56
C


ATOM
2386
CB
ALA
A
168
19.014
13.978
28.698
1.00
11.45
C


ATOM
2390
C
ALA
A
168
19.026
11.631
27.894
1.00
10.08
C


ATOM
2391
O
ALA
A
168
19.441
10.860
28.771
1.00
10.97
O


ATOM
2392
N
GLY
A
169
18.020
11.315
27.108
1.00
9.80
N


ATOM
2394
CA
GLY
A
169
17.216
10.125
27.318
1.00
9.88
C


ATOM
2397
C
GLY
A
169
15.777
10.485
27.780
1.00
11.11
C


ATOM
2398
O
GLY
A
169
15.483
11.623
28.208
1.00
10.34
O


ATOM
2399
N
ASN
A
170
14.882
9.492
27.676
1.00
11.54
N


ATOM
2401
CA
ASN
A
170
13.483
9.636
28.090
1.00
11.53
C


ATOM
2403
CB
ASN
A
170
12.579
9.382
26.872
1.00
11.78
C


ATOM
2406
CG
ASN
A
170
12.911
10.285
25.682
1.00
13.65
C


ATOM
2407
OD1
ASN
A
170
13.358
11.427
25.856
1.00
14.26
O


ATOM
2408
ND2
ASN
A
170
12.666
9.791
24.465
1.00
10.92
N


ATOM
2411
C
ASN
A
170
13.116
8.658
29.184
1.00
12.69
C


ATOM
2412
O
ASN
A
170
12.046
8.036
29.123
1.00
12.66
O


ATOM
2413
N
SER
A
171
13.989
8.483
30.170
1.00
12.61
N


ATOM
2415
CA
SER
A
171
13.754
7.487
31.223
1.00
13.48
C


ATOM
2417
CB
SER
A
171
15.025
6.692
31.423
1.00
14.78
C


ATOM
2420
OG
SER
A
171
15.277
5.967
30.233
1.00
13.11
O


ATOM
2422
C
SER
A
171
13.308
8.147
32.538
1.00
13.22
C


ATOM
2423
O
SER
A
171
13.429
7.526
33.583
1.00
14.40
O


ATOM
2424
N
GLY
A
172
12.811
9.371
32.496
1.00
14.32
N


ATOM
2426
CA
GLY
A
172
12.428
10.098
33.710
1.00
14.13
C


ATOM
2429
C
GLY
A
172
11.127
9.606
34.292
1.00
15.66
C


ATOM
2430
O
GLY
A
172
10.473
8.814
33.614
1.00
15.22
O


ATOM
2431
N
SER
A
173
10.681
10.134
35.424
1.00
14.94
N


ATOM
2433
CA
SER
A
173
11.269
11.278
36.134
1.00
16.57
C


ATOM
2435
CB
SER
A
173
10.144
12.174
36.639
1.00
17.91
C


ATOM
2438
OG
SER
A
173
9.384
11.435
37.607
1.00
18.04
O


ATOM
2440
C
SER
A
173
12.196
10.908
37.265
1.00
16.47
C


ATOM
2441
O
SER
A
173
12.751
11.790
37.970
1.00
15.55
O


ATOM
2442
N
GLY
A
174
12.476
9.615
37.359
1.00
15.06
N


ATOM
2444
CA
GLY
A
174
13.318
9.075
38.400
1.00
16.35
C


ATOM
2447
C
GLY
A
174
14.715
9.629
38.233
1.00
17.64
C


ATOM
2448
O
GLY
A
174
15.159
9.906
37.086
1.00
17.46
O


ATOM
2449
N
SER
A
175
15.404
9.827
39.351
1.00
17.08
N


ATOM
2451
CA
SER
A
175
16.752
10.404
39.336
1.00
18.22
C


ATOM
2453
CB
SER
A
175
17.129
10.794
40.759
1.00
19.80
C


ATOM
2456
OG
SER
A
175
16.121
11.654
41.308
1.00
21.20
O


ATOM
2458
C
SER
A
175
17.783
9.457
38.777
1.00
16.85
C


ATOM
2459
O
SER
A
175
17.638
8.238
38.884
1.00
15.37
O


ATOM
2460
N
ASN
A
176
18.838
10.010
38.168
1.00
16.57
N


ATOM
2462
CA
ASN
A
176
19.966
9.230
37.675
1.00
15.32
C


ATOM
2464
CB
ASN
A
176
20.679
8.475
38.817
1.00
17.33
C


ATOM
2467
CG
ASN
A
176
22.174
8.352
38.565
1.00
20.64
C


ATOM
2468
OD1
ASN
A
176
22.676
9.003
37.649
1.00
19.65
O


ATOM
2469
ND2
ASN
A
176
22.881
7.508
39.336
1.00
23.68
N


ATOM
2472
C
ASN
A
176
19.634
8.250
36.592
1.00
15.22
C


ATOM
2473
O
ASN
A
176
20.208
7.146
36.528
1.00
16.86
O


ATOM
2474
N
THR
A
177
18.718
8.639
35.723
1.00
14.53
N


ATOM
2476
CA
THR
A
177
18.299
7.815
34.612
1.00
14.30
C


ATOM
2478
CB
THR
A
177
16.768
7.831
34.488
1.00
13.61
C


ATOM
2480
OG1
THR
A
177
16.255
9.161
34.632
1.00
12.76
O


ATOM
2482
CG2
THR
A
177
16.053
7.001
35.629
1.00
14.89
C


ATOM
2486
C
THR
A
177
18.907
8.265
33.267
1.00
13.62
C


ATOM
2487
O
THR
A
177
18.555
7.704
32.213
1.00
14.34
O


ATOM
2488
N
ILE
A
178
19.736
9.305
33.324
1.00
12.33
N


ATOM
2490
CA
ILE
A
178
20.436
9.809
32.125
1.00
10.85
C


ATOM
2492
CB
ILE
A
178
21.473
10.886
32.543
1.00
11.28
C


ATOM
2494
CG1
ILE
A
178
22.118
11.576
31.337
1.00
11.95
C


ATOM
2497
CD1
ILE
A
178
22.981
12.833
31.722
1.00
12.82
C


ATOM
2501
CG2
ILE
A
178
22.550
10.300
33.406
1.00
11.10
C


ATOM
2505
C
ILE
A
178
21.057
8.663
31.350
1.00
10.78
C


ATOM
2506
O
ILE
A
178
21.582
7.715
31.950
1.00
10.96
O


ATOM
2507
N
GLY
A
179
20.973
8.706
30.032
1.00
9.07
N


ATOM
2509
CA
GLY
A
179
21.658
7.732
29.200
1.00
10.43
C


ATOM
2512
C
GLY
A
179
22.842
8.334
28.433
1.00
10.17
C


ATOM
2513
O
GLY
A
179
23.302
9.423
28.716
1.00
9.05
O


ATOM
2514
N
PHE
A
180
23.302
7.579
27.474
1.00
10.42
N


ATOM
2516
CA
PHE
A
180
24.566
7.766
26.771
1.00
9.90
C


ATOM
2518
CB
PHE
A
180
25.597
6.710
27.248
1.00
11.20
C


ATOM
2521
CG
PHE
A
180
25.926
6.868
28.691
1.00
10.40
C


ATOM
2522
CD1
PHE
A
180
25.089
6.304
29.673
1.00
12.43
C


ATOM
2524
CE1
PHE
A
180
25.346
6.539
31.013
1.00
13.80
C


ATOM
2526
CZ
PHE
A
180
26.377
7.353
31.379
1.00
14.77
C


ATOM
2528
CE2
PHE
A
180
27.195
7.936
30.428
1.00
14.15
C


ATOM
2530
CD2
PHE
A
180
26.951
7.710
29.086
1.00
12.50
C


ATOM
2532
C
PHE
A
180
24.307
7.663
25.268
1.00
10.49
C


ATOM
2533
O
PHE
A
180
23.545
6.804
24.833
1.00
11.55
O


ATOM
2534
N
PRO
A
181
25.023
8.448
24.458
1.00
9.50
N


ATOM
2535
CA
PRO
A
181
26.196
9.246
24.890
1.00
9.13
C


ATOM
2537
CB
PRO
A
181
26.937
9.496
23.524
1.00
9.24
C


ATOM
2540
CG
PRO
A
181
25.855
9.591
22.649
1.00
11.31
C


ATOM
2543
CD
PRO
A
181
24.874
8.496
23.002
1.00
10.03
C


ATOM
2546
C
PRO
A
181
25.983
10.570
25.610
1.00
10.79
C


ATOM
2547
O
PRO
A
181
26.959
11.251
25.986
1.00
9.76
O


ATOM
2548
N
GLY
A
182
24.743
11.019
25.752
1.00
10.07
N


ATOM
2550
CA
GLY
A
182
24.480
12.260
26.450
1.00
10.91
C


ATOM
2553
C
GLY
A
182
25.260
12.460
27.748
1.00
10.65
C


ATOM
2554
O
GLY
A
182
25.843
13.532
27.983
1.00
10.24
O


ATOM
2555
N
GLY
A
183
25.246
11.400
28.570
1.00
10.23
N


ATOM
2557
CA
GLY
A
183
25.860
11.370
29.888
1.00
11.34
C


ATOM
2560
C
GLY
A
183
27.370
11.393
29.929
1.00
11.75
C


ATOM
2561
O
GLY
A
183
27.957
11.394
31.025
1.00
10.33
O


ATOM
2562
N
LEU
A
184
28.007
11.351
28.761
1.00
11.62
N


ATOM
2564
CA
LEU
A
184
29.474
11.436
28.718
1.00
11.77
C


ATOM
2566
CB
LEU
A
184
30.022
11.038
27.369
1.00
10.76
C


ATOM
2569
CG
LEU
A
184
29.612
9.640
26.946
1.00
13.28
C


ATOM
2571
CD1
LEU
A
184
29.958
9.447
25.484
1.00
13.01
C


ATOM
2575
CD2
LEU
A
184
30.306
8.563
27.839
1.00
15.87
C


ATOM
2579
C
LEU
A
184
29.966
12.827
29.034
1.00
11.00
C


ATOM
2580
O
LEU
A
184
29.321
13.827
28.682
1.00
10.77
O


ATOM
2581
N
VAL
A
185
31.180
12.922
29.584
1.00
11.17
N


ATOM
2583
CA
VAL
A
185
31.673
14.225
29.979
1.00
10.77
C


ATOM
2585
CB
VAL
A
185
32.994
14.115
30.811
1.00
13.00
C


ATOM
2587
CG1
VAL
A
185
33.978
13.569
30.013
1.00
16.83
C


ATOM
2591
CG2
VAL
A
185
33.500
15.504
31.144
1.00
16.03
C


ATOM
2595
C
VAL
A
185
31.868
15.172
28.842
1.00
9.95
C


ATOM
2596
O
VAL
A
185
31.683
16.370
28.972
1.00
10.97
O


ATOM
2597
N
ASN
A
186
32.181
14.614
27.684
1.00
10.43
N


ATOM
2599
CA
ASN
A
186
32.483
15.376
26.517
1.00
11.79
C


ATOM
2601
CB
ASN
A
186
33.763
14.865
25.836
1.00
13.55
C


ATOM
2604
CG
ASN
A
186
35.029
15.152
26.660
1.00
14.82
C


ATOM
2605
OD1
ASN
A
186
35.093
16.110
27.370
1.00
19.19
O


ATOM
2606
ND2
ASN
A
186
36.021
14.326
26.517
1.00
21.64
N


ATOM
2609
C
ASN
A
186
31.305
15.525
25.536
1.00
11.85
C


ATOM
2610
O
ASN
A
186
31.485
15.915
24.384
1.00
11.39
O


ATOM
2611
N
ALA
A
187
30.108
15.138
25.977
1.00
10.51
N


ATOM
2613
CA
ALA
A
187
28.904
15.382
25.179
1.00
11.08
C


ATOM
2615
CB
ALA
A
187
28.189
14.150
24.848
1.00
11.19
C


ATOM
2619
C
ALA
A
187
27.984
16.317
25.975
1.00
10.66
C


ATOM
2620
O
ALA
A
187
27.878
16.147
27.186
1.00
11.05
O


ATOM
2621
N
VAL
A
188
27.318
17.248
25.288
1.00
9.26
N


ATOM
2623
CA
VAL
A
188
26.326
18.148
25.895
1.00
9.21
C


ATOM
2625
CB
VAL
A
188
26.120
19.418
25.046
1.00
9.41
C


ATOM
2627
CG1
VAL
A
188
25.035
20.249
25.661
1.00
7.57
C


ATOM
2631
CG2
VAL
A
188
27.448
20.164
24.893
1.00
10.01
C


ATOM
2635
C
VAL
A
188
24.996
17.346
25.984
1.00
9.78
C


ATOM
2636
O
VAL
A
188
24.349
17.137
24.959
1.00
10.45
O


ATOM
2637
N
ALA
A
189
24.572
16.989
27.200
1.00
8.67
N


ATOM
2639
CA
ALA
A
189
23.325
16.236
27.430
1.00
9.35
C


ATOM
2641
CB
ALA
A
189
23.379
15.510
28.763
1.00
10.27
C


ATOM
2645
C
ALA
A
189
22.197
17.214
27.451
1.00
8.43
C


ATOM
2646
O
ALA
A
189
22.179
18.183
28.238
1.00
9.35
O


ATOM
2647
N
VAL
A
190
21.182
16.948
26.651
1.00
9.14
N


ATOM
2649
CA
VAL
A
190
20.084
17.882
26.554
1.00
8.32
C


ATOM
2651
CB
VAL
A
190
19.843
18.296
25.119
1.00
8.58
C


ATOM
2653
CG1
VAL
A
190
18.731
19.309
25.052
1.00
11.50
C


ATOM
2657
CG2
VAL
A
190
21.084
18.873
24.482
1.00
9.20
C


ATOM
2661
C
VAL
A
190
18.791
17.317
27.087
1.00
9.51
C


ATOM
2662
O
VAL
A
190
18.340
16.256
26.625
1.00
9.51
O


ATOM
2663
N
ALA
A
191
18.236
17.973
28.093
1.00
9.50
N


ATOM
2665
CA
ALA
A
191
16.934
17.559
28.685
1.00
10.18
C


ATOM
2667
CB
ALA
A
191
16.868
18.057
30.134
1.00
8.69
C


ATOM
2671
C
ALA
A
191
15.800
18.184
27.900
1.00
10.24
C


ATOM
2672
O
ALA
A
191
16.007
19.182
27.249
1.00
10.28
O


ATOM
2673
N
ALA
A
192
14.570
17.659
28.021
1.00
11.22
N


ATOM
2675
CA
ALA
A
192
13.428
18.171
27.272
1.00
11.79
C


ATOM
2677
CB
ALA
A
192
12.593
17.012
26.728
1.00
13.73
C


ATOM
2681
C
ALA
A
192
12.499
19.027
28.134
1.00
11.54
C


ATOM
2682
O
ALA
A
192
12.048
18.549
29.179
1.00
11.30
O


ATOM
2683
N
LEU
A
193
12.222
20.238
27.673
1.00
11.58
N


ATOM
2685
CA
LEU
A
193
11.194
21.103
28.258
1.00
11.55
C


ATOM
2687
CB
LEU
A
193
11.519
22.561
28.037
1.00
12.06
C


ATOM
2690
CG
LEU
A
193
12.844
23.095
28.613
1.00
10.40
C


ATOM
2692
CD1
LEU
A
193
13.137
24.484
28.211
1.00
9.32
C


ATOM
2696
CD2
LEU
A
193
12.752
22.903
30.081
1.00
13.29
C


ATOM
2700
C
LEU
A
193
9.852
20.802
27.577
1.00
14.05
C


ATOM
2701
O
LEU
A
193
9.814
20.460
26.414
1.00
13.73
O


ATOM
2702
N
GLU
A
194
8.755
21.004
28.305
1.00
13.56
N


ATOM
2704
CA
GLU
A
194
7.422
21.049
27.647
1.00
13.63
C


ATOM
2706
CB
GLU
A
194
6.472
20.125
28.359
1.00
12.74
C


ATOM
2709
CG
GLU
A
194
6.320
20.410
29.837
1.00
16.62
C


ATOM
2712
CD
GLU
A
194
5.490
19.404
30.603
1.00
19.10
C


ATOM
2713
OE1
GLU
A
194
5.288
18.280
30.118
1.00
20.53
O


ATOM
2714
OE2
GLU
A
194
5.153
19.744
31.765
1.00
17.20
O


ATOM
2715
C
GLU
A
194
6.934
22.460
27.699
1.00
14.48
C


ATOM
2716
O
GLU
A
194
7.502
23.275
28.431
1.00
13.32
O


ATOM
2717
N
ASN
A
195
5.862
22.778
26.954
1.00
14.74
N


ATOM
2719
CA
ASN
A
195
5.416
24.169
26.857
1.00
15.00
C


ATOM
2721
CB
ASN
A
195
4.773
24.467
25.479
1.00
16.14
C


ATOM
2724
CG
ASN
A
195
4.612
25.970
25.198
1.00
16.46
C


ATOM
2725
OD1
ASN
A
195
5.236
26.803
25.855
1.00
14.84
O


ATOM
2726
ND2
ASN
A
195
3.775
26.318
24.208
1.00
13.91
N


ATOM
2729
C
ASN
A
195
4.439
24.440
27.945
1.00
16.03
C


ATOM
2730
O
ASN
A
195
3.256
24.692
27.662
1.00
15.98
O


ATOM
2731
N
VAL
A
196
4.904
24.381
29.170
1.00
16.13
N


ATOM
2733
CA
VAL
A
196
4.106
24.623
30.344
1.00
16.89
C


ATOM
2735
CB
VAL
A
196
3.739
23.348
31.019
1.00
18.38
C


ATOM
2737
CG1
VAL
A
196
3.058
23.613
32.326
1.00
20.19
C


ATOM
2741
CG2
VAL
A
196
2.922
22.415
30.070
1.00
18.32
C


ATOM
2745
C
VAL
A
196
4.991
25.380
31.307
1.00
17.79
C


ATOM
2746
O
VAL
A
196
6.215
25.147
31.344
1.00
17.15
O


ATOM
2747
N
GLN
A
197
4.410
26.305
32.055
1.00
17.34
N


ATOM
2749
CA
GLN
A
197
5.171
27.048
33.060
1.00
16.33
C


ATOM
2751
CB
GLN
A
197
4.838
28.518
33.012
1.00
16.74
C


ATOM
2754
CG
GLN
A
197
4.987
29.169
31.720
1.00
16.89
C


ATOM
2757
CD
GLN
A
197
6.455
29.357
31.343
1.00
18.48
C


ATOM
2758
OE1
GLN
A
197
7.216
30.009
32.096
1.00
14.66
O


ATOM
2759
NE2
GLN
A
197
6.850
28.769
30.223
1.00
15.37
N


ATOM
2762
C
GLN
A
197
4.907
26.538
34.467
1.00
17.79
C


ATOM
2763
O
GLN
A
197
3.778
26.114
34.825
1.00
18.50
O


ATOM
2764
N
GLN
A
198
5.977
26.409
35.232
1.00
17.07
N


ATOM
2766
CA
GLN
A
198
5.879
25.977
36.627
1.00
17.39
C


ATOM
2768
CB
GLN
A
198
5.865
24.485
36.778
1.00
17.84
C


ATOM
2771
CG
GLN
A
198
5.744
24.058
38.164
1.00
18.38
C


ATOM
2774
CD
GLN
A
198
5.797
22.558
38.413
1.00
25.24
C


ATOM
2775
OE1
GLN
A
198
6.612
21.813
37.815
1.00
26.03
O


ATOM
2776
NE2
GLN
A
198
4.927
22.090
39.323
1.00
29.00
N


ATOM
2779
C
GLN
A
198
6.998
26.623
37.362
1.00
17.51
C


ATOM
2780
O
GLN
A
198
8.156
26.681
36.904
1.00
16.23
O


ATOM
2781
N
ASN
A
199
6.655
27.147
38.520
1.00
18.31
N


ATOM
2783
CA
ASN
A
199
7.612
27.890
39.321
1.00
19.42
C


ATOM
2785
CB
ASN
A
199
8.676
26.953
39.915
1.00
19.53
C


ATOM
2788
CG
ASN
A
199
8.107
25.949
40.861
1.00
24.01
C


ATOM
2789
OD1
ASN
A
199
7.226
26.254
41.691
1.00
23.71
O


ATOM
2790
ND2
ASN
A
199
8.598
24.738
40.769
1.00
25.09
N


ATOM
2793
C
ASN
A
199
8.285
29.018
38.592
1.00
20.51
C


ATOM
2794
O
ASN
A
199
9.491
29.356
38.863
1.00
20.07
O


ATOM
2795
N
GLY
A
200
7.533
29.653
37.712
1.00
19.65
N


ATOM
2797
CA
GLY
A
200
8.005
30.820
36.991
1.00
20.16
C


ATOM
2800
C
GLY
A
200
8.883
30.609
35.774
1.00
19.26
C


ATOM
2801
O
GLY
A
200
9.347
31.577
35.177
1.00
18.58
O


ATOM
2802
N
THR
A
201
9.091
29.348
35.384
1.00
17.29
N


ATOM
2804
CA
THR
A
201
9.876
29.045
34.196
1.00
16.82
C


ATOM
2806
CB
THR
A
201
11.327
28.563
34.596
1.00
16.41
C


ATOM
2808
OG1
THR
A
201
11.309
27.246
35.174
1.00
17.63
O


ATOM
2810
CG2
THR
A
201
11.954
29.437
35.622
1.00
17.27
C


ATOM
2814
C
THR
A
201
9.248
27.935
33.389
1.00
15.50
C


ATOM
2815
O
THR
A
201
8.267
27.356
33.817
1.00
14.83
O


ATOM
2816
N
TYR
A
202
9.845
27.608
32.230
1.00
15.66
N


ATOM
2818
CA
TYR
A
202
9.469
26.407
31.571
1.00
14.51
C


ATOM
2820
CB
TYR
A
202
10.308
26.183
30.300
1.00
15.38
C


ATOM
2823
CG
TYR
A
202
9.853
27.053
29.177
1.00
12.00
C


ATOM
2824
CD1
TYR
A
202
8.775
26.682
28.359
1.00
12.96
C


ATOM
2826
CE1
TYR
A
202
8.344
27.510
27.364
1.00
13.55
C


ATOM
2828
CZ
TYR
A
202
8.995
28.682
27.157
1.00
12.70
C


ATOM
2829
OH
TYR
A
202
8.586
29.559
26.172
1.00
14.68
O


ATOM
2831
CE2
TYR
A
202
10.027
29.063
27.961
1.00
14.77
C


ATOM
2833
CD2
TYR
A
202
10.441
28.245
28.957
1.00
13.26
C


ATOM
2835
C
TYR
A
202
9.637
25.229
32.488
1.00
14.45
C


ATOM
2836
O
TYR
A
202
10.442
25.231
33.415
1.00
14.03
O


ATOM
2837
N
ARG
A
203
8.894
24.168
32.206
1.00
14.53
N


ATOM
2839
CA
ARG
A
203
8.939
22.971
32.988
1.00
13.25
C


ATOM
2841
CB
ARG
A
203
7.454
22.543
33.262
1.00
14.52
C


ATOM
2844
CG
ARG
A
203
7.315
21.347
34.102
1.00
14.19
C


ATOM
2847
CD
ARG
A
203
5.795
21.083
34.523
1.00
15.99
C


ATOM
2850
NE
ARG
A
203
5.730
20.106
35.572
1.00
17.45
N


ATOM
2852
CZ
ARG
A
203
5.729
18.806
35.402
1.00
17.33
C


ATOM
2853
NH1
ARG
A
203
5.762
18.306
34.191
1.00
15.77
N


ATOM
2856
NH2
ARG
A
203
5.741
17.996
36.447
1.00
18.53
N


ATOM
2859
C
ARG
A
203
9.595
21.805
32.251
1.00
12.75
C


ATOM
2860
O
ARG
A
203
9.285
21.570
31.076
1.00
12.82
O


ATOM
2861
N
VAL
A
204
10.505
21.090
32.907
1.00
12.18
N


ATOM
2863
CA
VAL
A
204
11.043
19.877
32.378
1.00
11.32
C


ATOM
2865
CB
VAL
A
204
12.141
19.338
33.296
1.00
11.73
C


ATOM
2867
CG1
VAL
A
204
12.765
18.035
32.735
1.00
11.27
C


ATOM
2871
CG2
VAL
A
204
13.229
20.373
33.437
1.00
12.71
C


ATOM
2875
C
VAL
A
204
9.925
18.831
32.221
1.00
12.28
C


ATOM
2876
O
VAL
A
204
9.172
18.549
33.185
1.00
12.08
O


ATOM
2877
N
ALA
A
205
9.855
18.166
31.063
1.00
12.11
N


ATOM
2879
CA
ALA
A
205
8.882
17.061
30.935
1.00
12.63
C


ATOM
2881
CB
ALA
A
205
8.725
16.612
29.473
1.00
14.20
C


ATOM
2885
C
ALA
A
205
9.205
15.894
31.835
1.00
14.44
C


ATOM
2886
O
ALA
A
205
10.338
15.546
32.067
1.00
12.72
O


ATOM
2887
N
ASP
A
206
8.160
15.251
32.353
1.00
12.46
N


ATOM
2889
CA
ASP
A
206
8.351
14.138
33.226
1.00
14.42
C


ATOM
2891
CB
ASP
A
206
7.015
13.579
33.660
1.00
15.15
C


ATOM
2894
CG
ASP
A
206
6.273
14.456
34.620
1.00
20.28
C


ATOM
2895
OD1
ASP
A
206
6.717
15.552
35.015
1.00
16.78
O


ATOM
2896
OD2
ASP
A
206
5.164
14.025
35.032
1.00
21.17
O


ATOM
2897
C
ASP
A
206
9.161
13.020
32.556
1.00
13.89
C


ATOM
2898
O
ASP
A
206
9.920
12.348
33.229
1.00
15.04
O


ATOM
2899
N
PHE
A
207
9.016
12.821
31.246
1.00
11.78
N


ATOM
2901
CA
PHE
A
207
9.710
11.721
30.612
1.00
13.96
C


ATOM
2903
CB
PHE
A
207
9.163
11.360
29.213
1.00
13.87
C


ATOM
2906
CG
PHE
A
207
9.290
12.439
28.191
1.00
14.05
C


ATOM
2907
CD1
PHE
A
207
10.521
12.704
27.630
1.00
13.46
C


ATOM
2909
CE1
PHE
A
207
10.677
13.677
26.709
1.00
14.99
C


ATOM
2911
CZ
PHE
A
207
9.577
14.463
26.305
1.00
12.88
C


ATOM
2913
CE2
PHE
A
207
8.325
14.173
26.841
1.00
15.57
C


ATOM
2915
CD2
PHE
A
207
8.199
13.191
27.787
1.00
14.48
C


ATOM
2917
C
PHE
A
207
11.220
11.917
30.546
1.00
12.81
C


ATOM
2918
O
PHE
A
207
11.950
10.945
30.339
1.00
12.43
O


ATOM
2919
N
SER
A
208
11.670
13.163
30.626
1.00
13.24
N


ATOM
2921
CA
SER
A
208
13.099
13.459
30.389
1.00
12.69
C


ATOM
2923
CB
SER
A
208
13.277
14.980
30.333
1.00
13.69
C


ATOM
2926
OG
SER
A
208
14.593
15.399
30.016
1.00
11.00
O


ATOM
2928
C
SER
A
208
13.997
12.799
31.432
1.00
11.85
C


ATOM
2929
O
SER
A
208
13.726
12.841
32.612
1.00
12.56
O


ATOM
2930
N
SER
A
209
15.095
12.168
31.001
1.00
11.34
N


ATOM
2932
CA
SER
A
209
15.961
11.503
31.941
1.00
12.26
C


ATOM
2934
CB
SER
A
209
17.003
10.655
31.240
1.00
10.68
C


ATOM
2937
OG
SER
A
209
16.442
9.566
30.515
1.00
11.25
O


ATOM
2939
C
SER
A
209
16.666
12.506
32.852
1.00
11.42
C


ATOM
2940
O
SER
A
209
17.108
13.568
32.420
1.00
12.21
O


ATOM
2941
N
ARG
A
210
16.797
12.128
34.107
1.00
13.04
N


ATOM
2943
CA
ARG
A
210
17.480
12.973
35.089
1.00
12.02
C


ATOM
2945
CB
ARG
A
210
16.783
12.925
36.439
1.00
11.62
C


ATOM
2948
CG
ARG
A
210
15.644
13.914
36.659
1.00
10.95
C


ATOM
2951
CD
ARG
A
210
14.531
13.936
35.593
1.00
12.61
C


ATOM
2954
NE
ARG
A
210
13.496
14.936
35.948
1.00
13.59
N


ATOM
2956
CZ
ARG
A
210
12.450
15.250
35.214
1.00
14.23
C


ATOM
2957
NH1
ARG
A
210
12.267
14.655
34.035
1.00
14.26
N


ATOM
2960
NH2
ARG
A
210
11.550
16.168
35.652
1.00
13.18
N


ATOM
2963
C
ARG
A
210
18.906
12.544
35.328
1.00
11.40
C


ATOM
2964
O
ARG
A
210
19.201
11.401
35.281
1.00
12.42
O


ATOM
2965
N
GLY
A
211
19.756
13.520
35.625
1.00
13.31
N


ATOM
2967
CA
GLY
A
211
21.140
13.289
35.977
1.00
11.79
C


ATOM
2970
C
GLY
A
211
21.263
12.757
37.395
1.00
13.79
C


ATOM
2971
O
GLY
A
211
20.286
12.467
38.054
1.00
13.33
O


ATOM
2972
N
ASN
A
212
22.508
12.644
37.831
1.00
12.68
N


ATOM
2974
CA
ASN
A
212
22.852
12.132
39.139
1.00
13.86
C


ATOM
2976
CB
ASN
A
212
24.300
11.647
39.062
1.00
14.05
C


ATOM
2979
CG
ASN
A
212
24.801
11.076
40.380
1.00
15.77
C


ATOM
2980
OD1
ASN
A
212
24.034
10.961
41.330
1.00
20.22
O


ATOM
2981
ND2
ASN
A
212
26.057
10.638
40.402
1.00
21.14
N


ATOM
2984
C
ASN
A
212
22.711
13.289
40.154
1.00
13.72
C


ATOM
2985
O
ASN
A
212
23.466
14.254
40.141
1.00
13.40
O


ATOM
2986
N
PRO
A
213
21.803
13.173
41.121
1.00
14.98
N


ATOM
2987
CA
PRO
A
213
21.672
14.248
42.125
1.00
16.29
C


ATOM
2989
CB
PRO
A
213
20.586
13.722
43.065
1.00
17.58
C


ATOM
2992
CG
PRO
A
213
19.803
12.803
42.224
1.00
16.96
C


ATOM
2995
CD
PRO
A
213
20.863
12.072
41.386
1.00
15.73
C


ATOM
2998
C
PRO
A
213
22.966
14.577
42.864
1.00
16.46
C


ATOM
2999
O
PRO
A
213
23.194
15.766
43.089
1.00
18.16
O


ATOM
3000
N
ALA
A
214
23.809
13.573
43.084
1.00
17.81
N


ATOM
3002
CA
ALA
A
214
25.058
13.706
43.843
1.00
18.79
C


ATOM
3004
CB
ALA
A
214
25.746
12.336
44.004
1.00
19.37
C


ATOM
3008
C
ALA
A
214
26.022
14.653
43.188
1.00
18.92
C


ATOM
3009
O
ALA
A
214
26.872
15.225
43.890
1.00
17.86
O


ATOM
3010
N
THR
A
215
25.899
14.879
41.869
1.00
17.03
N


ATOM
3012
CA
THR
A
215
26.868
15.751
41.205
1.00
16.66
C


ATOM
3014
CB
THR
A
215
27.741
14.942
40.212
1.00
18.38
C


ATOM
3016
OG1
THR
A
215
26.907
14.218
39.271
1.00
15.43
O


ATOM
3018
CG2
THR
A
215
28.532
13.928
40.970
1.00
18.05
C


ATOM
3022
C
THR
A
215
26.278
16.952
40.479
1.00
16.40
C


ATOM
3023
O
THR
A
215
26.955
17.636
39.745
1.00
16.41
O


ATOM
3024
N
ALA
A
216
25.035
17.244
40.773
1.00
14.91
N


ATOM
3026
CA
ALA
A
216
24.408
18.446
40.249
1.00
16.14
C


ATOM
3028
CB
ALA
A
216
22.944
18.182
39.938
1.00
16.49
C


ATOM
3032
C
ALA
A
216
24.479
19.548
41.271
1.00
17.73
C


ATOM
3033
O
ALA
A
216
24.240
19.279
42.445
1.00
21.27
O


ATOM
3034
N
GLY
A
217
24.699
20.763
40.840
1.00
16.83
N


ATOM
3036
CA
GLY
A
217
24.701
21.920
41.725
1.00
17.35
C


ATOM
3039
C
GLY
A
217
25.994
22.704
41.603
1.00
18.14
C


ATOM
3040
O
GLY
A
217
26.068
23.906
41.983
1.00
19.91
O


ATOM
3041
N
ASP
A
218
27.007
22.103
41.001
1.00
17.40
N


ATOM
3043
CA
ASP
A
218
28.294
22.767
40.941
1.00
16.68
C


ATOM
3045
CB
ASP
A
218
29.346
21.732
41.259
1.00
17.36
C


ATOM
3048
CG
ASP
A
218
29.393
20.585
40.244
1.00
20.24
C


ATOM
3049
OD1
ASP
A
218
28.520
20.437
39.333
1.00
17.05
O


ATOM
3050
OD2
ASP
A
218
30.286
19.734
40.356
1.00
20.78
O


ATOM
3051
C
ASP
A
218
28.648
23.526
39.664
1.00
15.65
C


ATOM
3052
O
ASP
A
218
29.638
24.231
39.613
1.00
15.75
O


ATOM
3053
N
TYR
A
219
27.756
23.522
38.689
1.00
15.41
N


ATOM
3055
CA
TYR
A
219
28.003
24.099
37.380
1.00
13.99
C


ATOM
3057
CB
TYR
A
219
27.987
25.607
37.463
1.00
14.94
C


ATOM
3060
CG
TYR
A
219
26.611
26.197
37.674
1.00
15.83
C


ATOM
3061
CD1
TYR
A
219
25.642
26.086
36.702
1.00
12.98
C


ATOM
3063
CE1
TYR
A
219
24.385
26.648
36.871
1.00
12.57
C


ATOM
3065
CZ
TYR
A
219
24.125
27.301
38.052
1.00
18.74
C


ATOM
3066
OH
TYR
A
219
22.947
27.921
38.286
1.00
18.11
O


ATOM
3068
CE2
TYR
A
219
25.090
27.438
39.021
1.00
19.74
C


ATOM
3070
CD2
TYR
A
219
26.309
26.869
38.843
1.00
19.78
C


ATOM
3072
C
TYR
A
219
29.346
23.584
36.756
1.00
14.17
C


ATOM
3073
O
TYR
A
219
29.978
24.283
35.984
1.00
14.67
O


ATOM
3074
N
ILE
A
220
29.676
22.330
37.031
1.00
13.56
N


ATOM
3076
CA
ILE
A
220
30.775
21.624
36.414
1.00
15.58
C


ATOM
3078
CB
ILE
A
220
31.961
21.438
37.355
1.00
15.45
C


ATOM
3080
CG1
ILE
A
220
32.502
22.809
37.761
1.00
20.96
C


ATOM
3083
CD1
ILE
A
220
33.397
22.711
39.015
1.00
23.15
C


ATOM
3087
CG2
ILE
A
220
33.087
20.625
36.671
1.00
18.47
C


ATOM
3091
C
ILE
A
220
30.227
20.278
35.956
1.00
13.00
C


ATOM
3092
O
ILE
A
220
29.633
19.505
36.691
1.00
12.94
O


ATOM
3093
N
ILE
A
221
30.482
19.995
34.684
1.00
15.43
N


ATOM
3095
CA
ILE
A
221
29.934
18.782
34.088
1.00
13.93
C


ATOM
3097
CB
ILE
A
221
29.793
18.960
32.541
1.00
12.79
C


ATOM
3099
CG1
ILE
A
221
28.733
19.979
32.164
1.00
12.82
C


ATOM
3102
CD1
ILE
A
221
27.361
19.711
32.673
1.00
12.70
C


ATOM
3106
CG2
ILE
A
221
29.513
17.651
31.889
1.00
14.76
C


ATOM
3110
C
ILE
A
221
30.836
17.584
34.406
1.00
14.63
C


ATOM
3111
O
ILE
A
221
32.059
17.583
34.006
1.00
14.50
O


ATOM
3112
N
GLN
A
222
30.246
16.602
35.097
1.00
13.31
N


ATOM
3114
CA
GLN
A
222
30.786
15.267
35.285
1.00
14.28
C


ATOM
3116
CB
GLN
A
222
30.772
14.858
36.759
1.00
16.33
C


ATOM
3119
CG
GLN
A
222
31.775
15.669
37.602
1.00
18.83
C


ATOM
3122
CD
GLN
A
222
31.204
16.936
38.223
1.00
21.01
C


ATOM
3123
OE1
GLN
A
222
29.998
17.058
38.469
1.00
22.83
O


ATOM
3124
NE2
GLN
A
222
32.089
17.874
38.517
1.00
23.39
N


ATOM
3127
C
GLN
A
222
29.935
14.289
34.460
1.00
14.17
C


ATOM
3128
O
GLN
A
222
28.921
14.664
33.874
1.00
12.09
O


ATOM
3129
N
GLU
A
223
30.438
13.079
34.313
1.00
13.56
N


ATOM
3131
CA
GLU
A
223
29.646
12.052
33.675
1.00
14.32
C


ATOM
3133
CB
GLU
A
223
30.455
10.767
33.682
1.00
14.62
C


ATOM
3136
CG
GLU
A
223
29.787
9.618
32.984
1.00
15.34
C


ATOM
3139
CD
GLU
A
223
30.759
8.474
32.706
1.00
16.41
C


ATOM
3140
OE1
GLU
A
223
31.400
8.407
31.648
1.00
19.83
O


ATOM
3141
OE2
GLU
A
223
30.808
7.645
33.564
1.00
19.76
O


ATOM
3142
C
GLU
A
223
28.315
11.911
34.451
1.00
12.77
C


ATOM
3143
O
GLU
A
223
28.294
11.981
35.679
1.00
13.97
O


ATOM
3144
N
ARG
A
224
27.210
11.785
33.700
1.00
11.67
N


ATOM
3146
CA
ARG
A
224
25.834
11.585
34.178
1.00
11.39
C


ATOM
3148
CB
ARG
A
224
25.730
10.549
35.305
1.00
12.52
C


ATOM
3151
CG
ARG
A
224
26.269
9.156
34.960
1.00
16.20
C


ATOM
3154
CD
ARG
A
224
25.988
8.116
36.088
1.00
19.39
C


ATOM
3157
NE
ARG
A
224
24.589
7.737
36.052
1.00
19.97
N


ATOM
3159
CZ
ARG
A
224
24.123
6.785
35.271
1.00
23.20
C


ATOM
3160
NH1
ARG
A
224
24.939
6.070
34.516
1.00
22.67
N


ATOM
3163
NH2
ARG
A
224
22.835
6.530
35.234
1.00
20.20
N


ATOM
3166
C
ARG
A
224
25.199
12.934
34.600
1.00
11.27
C


ATOM
3167
O
ARG
A
224
24.137
12.954
35.247
1.00
12.76
O


ATOM
3168
N
ASP
A
225
25.779
14.065
34.167
1.00
10.64
N


ATOM
3170
CA
ASP
A
225
25.141
15.322
34.440
1.00
11.57
C


ATOM
3172
CB
ASP
A
225
26.137
16.421
34.763
1.00
12.18
C


ATOM
3175
CG
ASP
A
225
26.783
16.290
36.115
1.00
11.47
C


ATOM
3176
OD1
ASP
A
225
26.396
15.431
36.930
1.00
12.63
O


ATOM
3177
OD2
ASP
A
225
27.738
17.024
36.366
1.00
12.98
O


ATOM
3178
C
ASP
A
225
24.386
15.833
33.187
1.00
11.72
C


ATOM
3179
O
ASP
A
225
24.880
15.761
32.028
1.00
10.65
O


ATOM
3180
N
ILE
A
226
23.195
16.366
33.428
1.00
10.89
N


ATOM
3182
CA
ILE
A
226
22.449
17.089
32.399
1.00
10.95
C


ATOM
3184
CB
ILE
A
226
20.988
17.379
32.826
1.00
10.97
C


ATOM
3186
CG1
ILE
A
226
20.296
16.066
33.224
1.00
8.65
C


ATOM
3189
CD1
ILE
A
226
20.064
15.192
32.004
1.00
12.39
C


ATOM
3193
CG2
ILE
A
226
20.260
18.217
31.828
1.00
12.31
C


ATOM
3197
C
ILE
A
226
23.117
18.428
32.195
1.00
8.74
C


ATOM
3198
O
ILE
A
226
23.561
19.076
33.123
1.00
10.81
O


ATOM
3199
N
GLU
A
227
23.254
18.849
30.971
1.00
9.24
N


ATOM
3201
CA
GLU
A
227
23.902
20.150
30.715
1.00
9.53
C


ATOM
3203
CB
GLU
A
227
24.905
19.979
29.553
1.00
8.94
C


ATOM
3206
CG
GLU
A
227
25.869
21.131
29.345
1.00
9.79
C


ATOM
3209
CD
GLU
A
227
27.217
20.634
28.789
1.00
11.40
C


ATOM
3210
OE1
GLU
A
227
27.527
19.431
28.857
1.00
8.56
O


ATOM
3211
OE2
GLU
A
227
27.953
21.441
28.255
1.00
11.05
O


ATOM
3212
C
GLU
A
227
22.936
21.331
30.450
1.00
9.10
C


ATOM
3213
O
GLU
A
227
23.053
22.402
31.074
1.00
10.35
O


ATOM
3214
N
VAL
A
228
21.984
21.152
29.553
1.00
8.61
N


ATOM
3216
CA
VAL
A
228
21.094
22.222
29.176
1.00
7.54
C


ATOM
3218
CB
VAL
A
228
21.567
23.010
27.946
1.00
5.96
C


ATOM
3220
CG1
VAL
A
228
22.834
23.726
28.263
1.00
8.28
C


ATOM
3224
CG2
VAL
A
228
21.680
22.127
26.740
1.00
8.08
C


ATOM
3228
C
VAL
A
228
19.727
21.575
28.904
1.00
7.83
C


ATOM
3229
O
VAL
A
228
19.663
20.362
28.747
1.00
9.43
O


ATOM
3230
N
SER
A
229
18.716
22.426
28.809
1.00
10.13
N


ATOM
3232
CA
SER
A
229
17.369
22.024
28.520
1.00
9.88
C


ATOM
3234
CB
SER
A
229
16.457
22.371
29.674
1.00
10.86
C


ATOM
3237
OG
SER
A
229
16.811
21.670
30.827
1.00
11.90
O


ATOM
3239
C
SER
A
229
16.865
22.806
27.305
1.00
11.07
C


ATOM
3240
O
SER
A
229
17.290
23.949
27.065
1.00
11.59
O


ATOM
3241
N
ALA
A
230
15.964
22.167
26.536
1.00
10.22
N


ATOM
3243
CA
ALA
A
230
15.337
22.824
25.379
1.00
9.98
C


ATOM
3245
CB
ALA
A
230
16.245
22.786
24.202
1.00
11.98
C


ATOM
3249
C
ALA
A
230
14.001
22.181
25.011
1.00
10.40
C


ATOM
3250
O
ALA
A
230
13.639
21.143
25.539
1.00
11.74
O


ATOM
3251
N
PRO
A
231
13.241
22.873
24.162
1.00
12.52
N


ATOM
3252
CA
PRO
A
231
11.915
22.369
23.794
1.00
11.45
C


ATOM
3254
CB
PRO
A
231
11.440
23.308
22.676
1.00
12.82
C


ATOM
3257
CG
PRO
A
231
12.076
24.609
23.005
1.00
12.47
C


ATOM
3260
CD
PRO
A
231
13.518
24.184
23.540
1.00
12.90
C


ATOM
3263
C
PRO
A
231
11.967
20.969
23.241
1.00
10.90
C


ATOM
3264
O
PRO
A
231
12.689
20.713
22.238
1.00
11.39
O


ATOM
3265
N
GLY
A
232
11.194
20.071
23.863
1.00
11.69
N


ATOM
3267
CA
GLY
A
232
11.218
18.675
23.478
1.00
12.12
C


ATOM
3270
C
GLY
A
232
9.857
17.972
23.431
1.00
14.63
C


ATOM
3271
O
GLY
A
232
9.814
16.852
22.976
1.00
17.38
O


ATOM
3272
N
ALA
A
233
8.775
18.641
23.807
1.00
13.80
N


ATOM
3274
CA
ALA
A
233
7.441
17.975
23.841
1.00
15.69
C


ATOM
3276
CB
ALA
A
233
6.812
18.002
25.228
1.00
15.50
C


ATOM
3280
C
ALA
A
233
6.565
18.649
22.794
1.00
15.70
C


ATOM
3281
O
ALA
A
233
6.479
19.892
22.725
1.00
15.71
O


ATOM
3282
N
SER
A
234
6.008
17.837
21.901
1.00
15.70
N


ATOM
3284
CA
SER
A
234
5.123
18.323
20.820
1.00
17.34
C


ATOM
3286
CB
SER
A
234
3.816
18.866
21.396
1.00
19.34
C


ATOM
3289
OG
SER
A
234
3.151
17.862
22.071
1.00
24.09
O


ATOM
3291
C
SER
A
234
5.746
19.302
19.869
1.00
16.08
C


ATOM
3292
O
SER
A
234
5.311
20.419
19.727
1.00
16.37
O


ATOM
3293
N
VAL
A
235
6.816
18.859
19.244
1.00
13.70
N


ATOM
3295
CA
VAL
A
235
7.597
19.607
18.309
1.00
13.30
C


ATOM
3297
CB
VAL
A
235
9.124
19.358
18.536
1.00
11.29
C


ATOM
3299
CG1
VAL
A
235
9.948
19.994
17.533
1.00
11.79
C


ATOM
3303
CG2
VAL
A
235
9.475
19.925
19.919
1.00
13.78
C


ATOM
3307
C
VAL
A
235
7.284
19.242
16.876
1.00
14.46
C


ATOM
3308
O
VAL
A
235
7.529
18.141
16.413
1.00
15.04
O


ATOM
3309
N
GLU
A
236
6.773
20.208
16.151
1.00
15.81
N


ATOM
3311
CA
GLU
A
236
6.539
20.075
14.717
1.00
15.96
C


ATOM
3313
CB
GLU
A
236
5.419
21.059
14.323
1.00
16.03
C


ATOM
3316
CG
GLU
A
236
5.033
21.028
12.863
1.00
18.99
C


ATOM
3319
CD
GLU
A
236
3.833
21.939
12.549
1.00
22.94
C


ATOM
3320
OE1
GLU
A
236
3.422
22.715
13.457
1.00
20.60
O


ATOM
3321
OE2
GLU
A
236
3.255
21.772
11.420
1.00
20.10
O


ATOM
3322
C
GLU
A
236
7.751
20.349
13.881
1.00
16.01
C


ATOM
3323
O
GLU
A
236
8.534
21.272
14.139
1.00
17.39
O


ATOM
3324
N
SER
A
237
8.023
19.462
12.905
1.00
14.14
N


ATOM
3326
CA
SER
A
237
9.105
19.655
12.025
1.00
14.47
C


ATOM
3328
CB
SER
A
237
10.410
19.109
12.632
1.00
11.88
C


ATOM
3331
OG
SER
A
237
11.513
19.504
11.921
1.00
11.55
O


ATOM
3333
C
SER
A
237
8.819
18.900
10.715
1.00
14.97
C


ATOM
3334
O
SER
A
237
7.699
18.345
10.543
1.00
16.60
O


ATOM
3335
N
THR
A
238
9.838
18.892
9.886
1.00
15.64
N


ATOM
3337
CA
THR
A
238
9.851
18.230
8.581
1.00
17.00
C


ATOM
3339
CB
THR
A
238
11.090
18.559
7.844
1.00
18.10
C


ATOM
3341
OG1
THR
A
238
12.267
18.454
8.677
1.00
16.55
O


ATOM
3343
CG2
THR
A
238
11.152
19.996
7.339
1.00
16.33
C


ATOM
3347
C
THR
A
238
9.752
16.703
8.759
1.00
19.28
C


ATOM
3348
O
THR
A
238
10.213
16.169
9.768
1.00
18.16
O


ATOM
3349
N
TRP
A
239
9.203
16.032
7.739
1.00
19.48
N


ATOM
3351
CA
TRP
A
239
8.933
14.587
7.765
1.00
18.83
C


ATOM
3353
CB
TRP
A
239
7.503
14.322
8.122
1.00
19.25
C


ATOM
3356
CG
TRP
A
239
7.182
12.954
8.642
1.00
19.71
C


ATOM
3357
CD1
TRP
A
239
6.343
12.009
8.085
1.00
23.51
C


ATOM
3359
NE1
TRP
A
239
6.263
10.907
8.899
1.00
22.49
N


ATOM
3361
CE2
TRP
A
239
7.081
11.114
9.985
1.00
21.32
C


ATOM
3362
CD2
TRP
A
239
7.651
12.400
9.853
1.00
20.74
C


ATOM
3363
CE3
TRP
A
239
8.529
12.864
10.851
1.00
18.32
C


ATOM
3365
CZ3
TRP
A
239
8.751
12.055
11.962
1.00
21.08
C


ATOM
3367
CH2
TRP
A
239
8.166
10.788
12.061
1.00
20.53
C


ATOM
3369
CZ2
TRP
A
239
7.349
10.289
11.079
1.00
22.21
C


ATOM
3371
C
TRP
A
239
9.325
13.931
6.461
1.00
17.68
C


ATOM
3372
O
TRP
A
239
9.417
14.577
5.423
1.00
19.48
O


ATOM
3373
N
TYR
A
240
9.679
12.642
6.550
1.00
17.80
N


ATOM
3375
CA
TYR
A
240
10.332
11.947
5.485
1.00
17.76
C


ATOM
3377
CB
TYR
A
240
10.862
10.568
5.938
1.00
20.08
C


ATOM
3380
CG
TYR
A
240
9.864
9.469
6.036
1.00
17.92
C


ATOM
3381
CD1
TYR
A
240
8.997
9.367
7.097
1.00
20.96
C


ATOM
3383
CE1
TYR
A
240
8.113
8.325
7.179
1.00
22.25
C


ATOM
3385
CZ
TYR
A
240
8.150
7.317
6.171
1.00
27.67
C


ATOM
3386
OH
TYR
A
240
7.269
6.247
6.207
1.00
28.63
O


ATOM
3388
CE2
TYR
A
240
9.034
7.397
5.159
1.00
25.36
C


ATOM
3390
CD2
TYR
A
240
9.884
8.442
5.084
1.00
24.19
C


ATOM
3392
C
TYR
A
240
9.453
11.746
4.253
1.00
19.14
C


ATOM
3393
O
TYR
A
240
9.992
11.532
3.199
1.00
19.72
O


ATOM
3394
N
THR
A
241
8.172
11.873
4.401
1.00
21.32
N


ATOM
3396
CA
THR
A
241
7.309
11.777
3.214
1.00
24.31
C


ATOM
3398
CB
THR
A
241
6.015
11.129
3.549
1.00
24.08
C


ATOM
3400
OG1
THR
A
241
5.443
11.711
4.720
1.00
25.38
O


ATOM
3402
CG2
THR
A
241
6.238
9.670
3.924
1.00
28.14
C


ATOM
3406
C
THR
A
241
7.020
13.113
2.587
1.00
26.01
C


ATOM
3407
O
THR
A
241
6.175
13.200
1.682
1.00
27.85
O


ATOM
3408
N
GLY
A
242
7.684
14.159
3.041
1.00
26.13
N


ATOM
3410
CA
GLY
A
242
7.445
15.480
2.484
1.00
26.65
C


ATOM
3413
C
GLY
A
242
6.544
16.381
3.303
1.00
25.41
C


ATOM
3414
O
GLY
A
242
6.524
17.583
3.068
1.00
29.27
O


ATOM
3415
N
GLY
A
243
5.786
15.891
4.246
1.00
23.67
N


ATOM
3417
CA
GLY
A
243
5.002
16.882
4.959
1.00
23.78
C


ATOM
3420
C
GLY
A
243
5.719
17.300
6.239
1.00
21.57
C


ATOM
3421
O
GLY
A
243
6.944
17.532
6.218
1.00
20.45
O


ATOM
3422
N
TYR
A
244
4.939
17.380
7.296
1.00
22.18
N


ATOM
3424
CA
TYR
A
244
5.404
17.745
8.651
1.00
21.26
C


ATOM
3426
CB
TYR
A
244
4.991
19.201
8.963
1.00
20.92
C


ATOM
3429
CG
TYR
A
244
5.467
20.078
7.877
1.00
20.48
C


ATOM
3430
CD1
TYR
A
244
4.696
20.312
6.749
1.00
20.98
C


ATOM
3432
CE1
TYR
A
244
5.167
21.082
5.733
1.00
21.20
C


ATOM
3434
CZ
TYR
A
244
6.396
21.604
5.785
1.00
20.81
C


ATOM
3435
OH
TYR
A
244
6.852
22.332
4.745
1.00
23.64
O


ATOM
3437
CE2
TYR
A
244
7.201
21.392
6.929
1.00
17.10
C


ATOM
3439
CD2
TYR
A
244
6.714
20.660
7.920
1.00
14.54
C


ATOM
3441
C
TYR
A
244
4.789
16.816
9.621
1.00
21.28
C


ATOM
3442
O
TYR
A
244
3.778
16.224
9.353
1.00
23.43
O


ATOM
3443
N
ASN
A
245
5.381
16.692
10.800
1.00
19.64
N


ATOM
3445
CA
ASN
A
245
4.866
15.855
11.825
1.00
18.70
C


ATOM
3447
CB
ASN
A
245
5.322
14.418
11.590
1.00
18.51
C


ATOM
3450
CG
ASN
A
245
4.644
13.438
12.450
1.00
18.67
C


ATOM
3451
OD1
ASN
A
245
3.509
13.611
12.925
1.00
21.26
O


ATOM
3452
ND2
ASN
A
245
5.350
12.323
12.689
1.00
22.00
N


ATOM
3455
C
ASN
A
245
5.304
16.401
13.154
1.00
16.91
C


ATOM
3456
O
ASN
A
245
6.289
17.194
13.212
1.00
17.80
O


ATOM
3457
N
THR
A
246
4.533
16.035
14.141
1.00
17.22
N


ATOM
3459
CA
THR
A
246
4.696
16.445
15.504
1.00
17.23
C


ATOM
3461
CB
THR
A
246
3.454
17.242
15.971
1.00
17.67
C


ATOM
3463
OG1
THR
A
246
3.368
18.458
15.223
1.00
18.24
O


ATOM
3465
CG2
THR
A
246
3.534
17.710
17.400
1.00
20.37
C


ATOM
3469
C
THR
A
246
4.993
15.244
16.374
1.00
17.33
C


ATOM
3470
O
THR
A
246
4.123
14.398
16.664
1.00
16.60
O


ATOM
3471
N
ILE
A
247
6.221
15.237
16.902
1.00
16.92
N


ATOM
3473
CA
ILE
A
247
6.655
14.201
17.813
1.00
16.77
C


ATOM
3475
CB
ILE
A
247
7.536
13.106
17.091
1.00
16.85
C


ATOM
3477
CG1
ILE
A
247
8.722
13.699
16.372
1.00
16.22
C


ATOM
3480
CD1
ILE
A
247
9.721
12.674
15.892
1.00
14.19
C


ATOM
3484
CG2
ILE
A
247
6.620
12.272
16.155
1.00
18.78
C


ATOM
3488
C
ILE
A
247
7.394
14.839
19.045
1.00
14.93
C


ATOM
3489
O
ILE
A
247
7.628
16.050
19.098
1.00
15.83
O


ATOM
3490
N
SER
A
248
7.659
14.020
20.030
1.00
14.46
N


ATOM
3492
CA
SER
A
248
8.268
14.443
21.285
1.00
14.38
C


ATOM
3494
CB
SER
A
248
7.262
14.230
22.426
1.00
14.68
C


ATOM
3497
OG
SER
A
248
6.080
14.988
22.271
1.00
16.96
O


ATOM
3499
C
SER
A
248
9.470
13.594
21.672
1.00
13.40
C


ATOM
3500
O
SER
A
248
9.626
12.448
21.227
1.00
12.06
O


ATOM
3501
N
GLY
A
249
10.306
14.151
22.557
1.00
11.90
N


ATOM
3503
CA
GLY
A
249
11.402
13.420
23.140
1.00
12.25
C


ATOM
3506
C
GLY
A
249
12.615
14.294
23.393
1.00
11.45
C


ATOM
3507
O
GLY
A
249
12.672
15.399
22.898
1.00
12.12
O


ATOM
3508
N
THR
A
250
13.607
13.759
24.103
1.00
10.99
N


ATOM
3510
CA
THR
A
250
14.898
14.476
24.200
1.00
9.84
C


ATOM
3512
CB
THR
A
250
15.835
13.937
25.257
1.00
9.57
C


ATOM
3514
OG1
THR
A
250
15.885
12.543
25.195
1.00
10.83
O


ATOM
3516
CG2
THR
A
250
15.260
14.258
26.612
1.00
11.12
C


ATOM
3520
C
THR
A
250
15.506
14.531
22.800
1.00
9.75
C


ATOM
3521
O
THR
A
250
16.312
15.393
22.517
1.00
11.31
O


ATOM
3522
N
SER
A
251
15.101
13.616
21.938
1.00
9.71
N


ATOM
3524
CA
SER
A
251
15.396
13.711
20.513
1.00
10.29
C


ATOM
3526
CB
SER
A
251
14.647
12.660
19.691
1.00
10.44
C


ATOM
3529
OG
SER
A
251
15.278
11.374
19.720
1.00
11.64
O


ATOM
3531
C
SER
A
251
15.132
15.058
19.850
1.00
10.60
C


ATOM
3532
O
SER
A
251
15.871
15.475
18.968
1.00
12.05
O


ATOM
3533
N
MET
A
252
14.023
15.698
20.246
1.00
11.35
N


ATOM
3535
CA
MET
A
252
13.598
16.937
19.649
1.00
12.05
C


ATOM
3537
CB
MET
A
252
12.081
17.000
19.803
1.00
10.74
C


ATOM
3540
CG
MET
A
252
11.275
16.163
18.811
1.00
16.05
C


ATOM
3543
SD
MET
A
252
11.445
14.393
18.855
1.00
12.67
S


ATOM
3544
CE
MET
A
252
12.360
14.105
17.508
1.00
14.70
C


ATOM
3548
C
MET
A
252
14.248
18.146
20.363
1.00
11.00
C


ATOM
3549
O
MET
A
252
14.372
19.200
19.805
1.00
10.97
O


ATOM
3550
N
ALA
A
253
14.615
17.978
21.628
1.00
11.44
N


ATOM
3552
CA
ALA
A
253
15.300
19.023
22.361
1.00
9.30
C


ATOM
3554
CB
ALA
A
253
15.282
18.677
23.852
1.00
8.63
C


ATOM
3558
C
ALA
A
253
16.733
19.234
21.842
1.00
9.29
C


ATOM
3559
O
ALA
A
253
17.173
20.367
21.599
1.00
10.72
O


ATOM
3560
N
THR
A
254
17.388
18.107
21.578
1.00
9.20
N


ATOM
3562
CA
THR
A
254
18.776
18.047
21.127
1.00
9.63
C


ATOM
3564
CB
THR
A
254
19.131
16.592
20.838
1.00
11.67
C


ATOM
3566
OG1
THR
A
254
19.001
15.823
22.036
1.00
9.34
O


ATOM
3568
CG2
THR
A
254
20.504
16.467
20.434
1.00
9.85
C


ATOM
3572
C
THR
A
254
19.041
18.957
19.927
1.00
9.01
C


ATOM
3573
O
THR
A
254
19.932
19.782
19.958
1.00
9.02
O


ATOM
3574
N
PRO
A
255
18.255
18.848
18.856
1.00
10.43
N


ATOM
3575
CA
PRO
A
255
18.491
19.725
17.711
1.00
9.72
C


ATOM
3577
CB
PRO
A
255
17.607
19.149
16.621
1.00
11.04
C


ATOM
3580
CG
PRO
A
255
16.511
18.430
17.368
1.00
11.23
C


ATOM
3583
CD
PRO
A
255
17.253
17.817
18.535
1.00
11.38
C


ATOM
3586
C
PRO
A
255
18.195
21.185
17.941
1.00
10.12
C


ATOM
3587
O
PRO
A
255
18.724
22.018
17.171
1.00
9.83
O


ATOM
3588
N
HIS
A
256
17.398
21.535
18.943
1.00
10.53
N


ATOM
3590
CA
HIS
A
256
17.233
22.947
19.219
1.00
10.79
C


ATOM
3592
CB
HIS
A
256
16.192
23.258
20.298
1.00
11.66
C


ATOM
3595
CG
HIS
A
256
14.748
23.136
19.820
1.00
12.48
C


ATOM
3596
ND1
HIS
A
256
14.086
21.930
19.732
1.00
11.32
N


ATOM
3598
CE1
HIS
A
256
12.849
22.144
19.296
1.00
14.50
C


ATOM
3600
NE2
HIS
A
256
12.709
23.434
19.040
1.00
12.79
N


ATOM
3602
CD2
HIS
A
256
13.889
24.074
19.348
1.00
12.20
C


ATOM
3604
C
HIS
A
256
18.572
23.451
19.658
1.00
11.12
C


ATOM
3605
O
HIS
A
256
18.977
24.554
19.302
1.00
12.50
O


ATOM
3606
N
VAL
A
257
19.264
22.660
20.474
1.00
10.13
N


ATOM
3608
CA
VAL
A
257
20.558
23.078
20.969
1.00
9.71
C


ATOM
3610
CB
VAL
A
257
20.966
22.264
22.219
1.00
10.12
C


ATOM
3612
CG1
VAL
A
257
22.407
22.589
22.653
1.00
10.84
C


ATOM
3616
CG2
VAL
A
257
19.951
22.451
23.324
1.00
11.41
C


ATOM
3620
C
VAL
A
257
21.632
22.993
19.871
1.00
10.99
C


ATOM
3621
O
VAL
A
257
22.483
23.871
19.782
1.00
11.55
O


ATOM
3622
N
ALA
A
258
21.664
21.933
19.070
1.00
10.95
N


ATOM
3624
CA
ALA
A
258
22.625
21.857
17.985
1.00
10.24
C


ATOM
3626
CB
ALA
A
258
22.459
20.568
17.233
1.00
9.96
C


ATOM
3630
C
ALA
A
258
22.446
23.027
17.061
1.00
11.33
C


ATOM
3631
O
ALA
A
258
23.426
23.621
16.598
1.00
10.81
O


ATOM
3632
N
GLY
A
259
21.191
23.351
16.799
1.00
11.66
N


ATOM
3634
CA
GLY
A
259
20.929
24.467
15.916
1.00
12.69
C


ATOM
3637
C
GLY
A
259
21.378
25.792
16.529
1.00
11.32
C


ATOM
3638
O
GLY
A
259
21.928
26.653
15.830
1.00
12.25
O


ATOM
3639
N
LEU
A
260
21.044
25.991
17.781
1.00
10.90
N


ATOM
3641
CA
LEU
A
260
21.453
27.191
18.479
1.00
11.40
C


ATOM
3643
CB
LEU
A
260
20.945
27.240
19.877
1.00
10.96
C


ATOM
3646
CG
LEU
A
260
21.372
28.483
20.678
1.00
11.84
C


ATOM
3648
CD1
LEU
A
260
20.781
29.683
20.099
1.00
16.22
C


ATOM
3652
CD2
LEU
A
260
20.948
28.349
22.174
1.00
12.84
C


ATOM
3656
C
LEU
A
260
22.984
27.291
18.477
1.00
12.53
C


ATOM
3657
O
LEU
A
260
23.558
28.384
18.205
1.00
11.28
O


ATOM
3658
N
ALA
A
261
23.644
26.153
18.745
1.00
11.39
N


ATOM
3660
CA
ALA
A
261
25.093
26.133
18.661
1.00
11.97
C


ATOM
3662
CB
ALA
A
261
25.604
24.716
18.889
1.00
13.65
C


ATOM
3666
C
ALA
A
261
25.607
26.638
17.292
1.00
12.95
C


ATOM
3667
O
ALA
A
261
26.563
27.413
17.203
1.00
12.12
O


ATOM
3668
N
ALA
A
262
24.998
26.144
16.229
1.00
11.02
N


ATOM
3670
CA
ALA
A
262
25.435
26.520
14.869
1.00
12.24
C


ATOM
3672
CB
ALA
A
262
24.731
25.669
13.828
1.00
12.25
C


ATOM
3676
C
ALA
A
262
25.200
28.002
14.619
1.00
13.52
C


ATOM
3677
O
ALA
A
262
26.045
28.684
13.996
1.00
13.55
O


ATOM
3678
N
LYS
A
263
24.091
28.505
15.135
1.00
13.42
N


ATOM
3680
CA
LYS
A
263
23.730
29.913
15.021
1.00
13.54
C


ATOM
3682
CB
LYS
A
263
22.319
30.164
15.511
1.00
14.20
C


ATOM
3685
CG
LYS
A
263
21.797
31.603
15.356
1.00
13.78
C


ATOM
3688
CD
LYS
A
263
20.412
31.725
15.984
1.00
15.37
C


ATOM
3691
CE
LYS
A
263
19.936
33.158
16.011
1.00
17.70
C


ATOM
3694
NZ
LYS
A
263
19.671
33.740
14.618
1.00
14.92
N


ATOM
3698
C
LYS
A
263
24.754
30.774
15.736
1.00
12.81
C


ATOM
3699
O
LYS
A
263
25.225
31.781
15.148
1.00
13.25
O


ATOM
3700
N
ILE
A
264
25.133
30.393
16.961
1.00
12.96
N


ATOM
3702
CA
ILE
A
264
26.107
31.136
17.723
1.00
12.47
C


ATOM
3704
CB
ILE
A
264
26.209
30.579
19.150
1.00
12.83
C


ATOM
3706
CG1
ILE
A
264
24.895
30.799
19.927
1.00
12.96
C


ATOM
3709
CD1
ILE
A
264
24.829
30.017
21.259
1.00
13.94
C


ATOM
3713
CG2
ILE
A
264
27.382
31.146
19.925
1.00
12.54
C


ATOM
3717
C
ILE
A
264
27.478
31.081
17.018
1.00
12.78
C


ATOM
3718
O
ILE
A
264
28.147
32.110
16.835
1.00
12.72
O


ATOM
3719
N
TRP
A
265
27.843
29.890
16.527
1.00
12.74
N


ATOM
3721
CA
TRP
A
265
29.194
29.711
15.984
1.00
13.37
C


ATOM
3723
CB
TRP
A
265
29.445
28.252
15.665
1.00
13.60
C


ATOM
3726
CG
TRP
A
265
30.859
27.836
15.758
1.00
12.37
C


ATOM
3727
CD1
TRP
A
265
31.987
28.624
15.964
1.00
12.89
C


ATOM
3729
NE1
TRP
A
265
33.101
27.823
16.063
1.00
14.49
N


ATOM
3731
CE2
TRP
A
265
32.705
26.514
15.998
1.00
16.16
C


ATOM
3732
CD2
TRP
A
265
31.313
26.493
15.796
1.00
12.95
C


ATOM
3733
CE3
TRP
A
265
30.679
25.254
15.657
1.00
14.36
C


ATOM
3735
CZ3
TRP
A
265
31.472
24.110
15.707
1.00
12.68
C


ATOM
3737
CH2
TRP
A
265
32.809
24.180
15.941
1.00
12.39
C


ATOM
3739
CZ2
TRP
A
265
33.462
25.359
16.069
1.00
15.97
C


ATOM
3741
C
TRP
A
265
29.378
30.560
14.732
1.00
13.71
C


ATOM
3742
O
TRP
A
265
30.423
31.117
14.530
1.00
17.12
O


ATOM
3743
N
SER
A
266
28.330
30.599
13.922
1.00
15.25
N


ATOM
3745
CA
SER
A
266
28.307
31.345
12.676
1.00
14.75
C


ATOM
3747
CB
SER
A
266
27.000
31.130
11.945
1.00
15.47
C


ATOM
3750
OG
SER
A
266
26.987
31.917
10.741
1.00
18.19
O


ATOM
3752
C
SER
A
266
28.498
32.835
12.962
1.00
16.77
C


ATOM
3753
O
SER
A
266
29.218
33.537
12.227
1.00
16.07
O


ATOM
3754
N
ALA
A
267
27.903
33.307
14.053
1.00
15.63
N


ATOM
3756
CA
ALA
A
267
28.046
34.711
14.450
1.00
17.65
C


ATOM
3758
CB
ALA
A
267
26.911
35.093
15.318
1.00
19.01
C


ATOM
3762
C
ALA
A
267
29.405
35.056
15.092
1.00
19.17
C


ATOM
3763
O
ALA
A
267
29.744
36.249
15.277
1.00
18.66
O


ATOM
3764
N
ASN
A
268
30.188
34.053
15.465
1.00
17.88
N


ATOM
3766
CA
ASN
A
268
31.557
34.318
15.886
1.00
20.05
C


ATOM
3768
CB
ASN
A
268
31.688
34.603
17.324
1.00
20.47
C


ATOM
3771
CG
ASN
A
268
33.120
35.029
17.701
1.00
23.58
C


ATOM
3772
OD1
ASN
A
268
34.017
35.076
16.860
1.00
24.59
O


ATOM
3773
ND2
ASN
A
268
33.327
35.286
18.961
1.00
27.93
N


ATOM
3776
C
ASN
A
268
32.437
33.156
15.520
1.00
18.98
C


ATOM
3777
O
ASN
A
268
32.651
32.264
16.321
1.00
17.09
O


ATOM
3778
N
THR
A
269
32.915
33.169
14.289
1.00
19.21
N


ATOM
3780
CA
THR
A
269
33.696
32.076
13.772
1.00
19.25
C


ATOM
3782
CB
THR
A
269
33.838
32.153
12.207
1.00
21.27
C


ATOM
3784
OG1
THR
A
269
34.379
33.443
11.889
1.00
21.88
O


ATOM
3786
CG2
THR
A
269
32.479
32.127
11.565
1.00
23.50
C


ATOM
3790
C
THR
A
269
35.055
32.025
14.380
1.00
19.11
C


ATOM
3791
O
THR
A
269
35.761
31.117
14.072
1.00
19.77
O


ATOM
3792
N
SER
A
270
35.435
32.929
15.297
1.00
19.17
N


ATOM
3794
CA
SER
A
270
36.732
32.774
15.936
1.00
19.54
C


ATOM
3796
CB
ASER
A
270
37.231
34.105
16.454
0.50
19.48
C


ATOM
3797
CB
BSER
A
270
37.264
34.116
16.400
0.50
19.66
C


ATOM
3802
OG
ASER
A
270
36.235
34.749
17.225
0.50
21.34
O


ATOM
3803
OG
BSER
A
270
37.628
34.922
15.280
0.50
22.88
O


ATOM
3806
C
SER
A
270
36.679
31.795
17.100
1.00
17.42
C


ATOM
3807
O
SER
A
270
37.688
31.382
17.637
1.00
19.69
O


ATOM
3808
N
LEU
A
271
35.493
31.392
17.484
1.00
15.84
N


ATOM
3810
CA
LEU
A
271
35.364
30.456
18.595
1.00
13.46
C


ATOM
3812
CB
LEU
A
271
33.910
30.345
19.026
1.00
14.65
C


ATOM
3815
CG
LEU
A
271
33.146
31.575
19.466
1.00
15.68
C


ATOM
3817
CD1
LEU
A
271
31.659
31.196
19.782
1.00
18.69
C


ATOM
3821
CD2
LEU
A
271
33.807
32.216
20.685
1.00
21.20
C


ATOM
3825
C
LEU
A
271
35.806
29.028
18.220
1.00
12.04
C


ATOM
3826
O
LEU
A
271
35.573
28.546
17.091
1.00
11.86
O


ATOM
3827
N
SER
A
272
36.323
28.335
19.220
1.00
12.57
N


ATOM
3829
CA
SER
A
272
36.545
26.919
19.145
1.00
11.98
C


ATOM
3831
CB
SER
A
272
37.710
26.489
20.040
1.00
12.49
C


ATOM
3834
OG
SER
A
272
37.433
26.778
21.421
1.00
12.53
O


ATOM
3836
C
SER
A
272
35.275
26.244
19.663
1.00
11.47
C


ATOM
3837
O
SER
A
272
34.372
26.889
20.269
1.00
9.92
O


ATOM
3838
N
HIS
A
273
35.225
24.937
19.454
1.00
11.48
N


ATOM
3840
CA
HIS
A
273
34.074
24.187
19.920
1.00
11.75
C


ATOM
3842
CB
HIS
A
273
34.187
22.750
19.445
1.00
13.59
C


ATOM
3845
CG
HIS
A
273
35.160
21.921
20.197
1.00
13.01
C


ATOM
3846
ND1
HIS
A
273
36.456
22.312
20.379
1.00
11.73
N


ATOM
3848
CE1
HIS
A
273
37.072
21.402
21.124
1.00
13.52
C


ATOM
3850
NE2
HIS
A
273
36.243
20.394
21.343
1.00
12.58
N


ATOM
3852
CD2
HIS
A
273
35.039
20.708
20.787
1.00
14.38
C


ATOM
3854
C
HIS
A
273
33.887
24.268
21.427
1.00
11.79
C


ATOM
3855
O
HIS
A
273
32.723
24.252
21.930
1.00
10.83
O


ATOM
3856
N
SER
A
274
34.975
24.276
22.191
1.00
10.50
N


ATOM
3858
CA
SER
A
274
34.813
24.319
23.643
1.00
10.49
C


ATOM
3860
CB
SER
A
274
36.035
23.803
24.397
1.00
13.18
C


ATOM
3863
OG
SER
A
274
37.161
24.639
24.166
1.00
12.23
O


ATOM
3865
C
SER
A
274
34.478
25.702
24.113
1.00
12.50
C


ATOM
3866
O
SER
A
274
33.783
25.849
25.123
1.00
11.08
O


ATOM
3867
N
GLN
A
275
34.919
26.739
23.418
1.00
11.42
N


ATOM
3869
CA
GLN
A
275
34.425
28.072
23.784
1.00
11.21
C


ATOM
3871
CB
GLN
A
275
35.132
29.188
22.999
1.00
11.23
C


ATOM
3874
CG
GLN
A
275
36.608
29.464
23.375
1.00
13.15
C


ATOM
3877
CD
GLN
A
275
37.192
30.473
22.413
1.00
13.10
C


ATOM
3878
OE1
GLN
A
275
37.323
30.182
21.228
1.00
15.35
O


ATOM
3879
NE2
GLN
A
275
37.555
31.677
22.923
1.00
11.91
N


ATOM
3882
C
GLN
A
275
32.898
28.177
23.457
1.00
11.10
C


ATOM
3883
O
GLN
A
275
32.150
28.776
24.217
1.00
10.23
O


ATOM
3884
N
LEU
A
276
32.492
27.556
22.340
1.00
10.08
N


ATOM
3886
CA
LEU
A
276
31.067
27.521
21.932
1.00
10.49
C


ATOM
3888
CB
LEU
A
276
30.909
26.866
20.582
1.00
10.65
C


ATOM
3891
CG
LEU
A
276
29.466
26.606
20.164
1.00
8.36
C


ATOM
3893
CD1
LEU
A
276
28.715
27.856
20.038
1.00
9.01
C


ATOM
3897
CD2
LEU
A
276
29.489
25.869
18.849
1.00
11.63
C


ATOM
3901
C
LEU
A
276
30.272
26.790
23.041
1.00
11.06
C


ATOM
3902
O
LEU
A
276
29.226
27.252
23.494
1.00
10.87
O


ATOM
3903
N
ARG
A
277
30.783
25.671
23.524
1.00
9.13
N


ATOM
3905
CA
ARG
A
277
30.104
24.934
24.560
1.00
11.95
C


ATOM
3907
CB
ARG
A
277
30.915
23.671
24.913
1.00
10.95
C


ATOM
3910
CG
ARG
A
277
30.335
22.793
26.018
1.00
13.22
C


ATOM
3913
CD
ARG
A
277
31.103
21.490
26.229
1.00
13.28
C


ATOM
3916
NE
ARG
A
277
30.415
20.601
27.149
1.00
13.25
N


ATOM
3918
CZ
ARG
A
277
30.892
19.416
27.518
1.00
14.34
C


ATOM
3919
NH1
ARG
A
277
32.089
19.014
27.110
1.00
14.38
N


ATOM
3922
NH2
ARG
A
277
30.190
18.660
28.345
1.00
13.28
N


ATOM
3925
C
ARG
A
277
29.974
25.722
25.858
1.00
12.12
C


ATOM
3926
O
ARG
A
277
28.949
25.722
26.538
1.00
12.16
O


ATOM
3927
N
THR
A
278
31.023
26.469
26.175
1.00
12.13
N


ATOM
3929
CA
THR
A
278
31.049
27.295
27.359
1.00
11.62
C


ATOM
3931
CB
THR
A
278
32.461
27.918
27.485
1.00
12.51
C


ATOM
3933
OG1
THR
A
278
33.379
26.892
27.846
1.00
15.78
O


ATOM
3935
CG2
THR
A
278
32.551
28.829
28.624
1.00
17.34
C


ATOM
3939
C
THR
A
278
29.990
28.388
27.287
1.00
10.34
C


ATOM
3940
O
THR
A
278
29.372
28.715
28.294
1.00
11.22
O


ATOM
3941
N
GLU
A
279
29.775
28.943
26.101
1.00
10.24
N


ATOM
3943
CA
GLU
A
279
28.804
30.034
25.920
1.00
10.36
C


ATOM
3945
CB
GLU
A
279
29.090
30.744
24.607
1.00
13.04
C


ATOM
3948
CG
GLU
A
279
28.155
31.799
24.172
1.00
13.67
C


ATOM
3951
CD
GLU
A
279
27.827
32.923
25.148
1.00
11.41
C


ATOM
3952
OE1
GLU
A
279
28.413
33.084
26.245
1.00
12.14
O


ATOM
3953
OE2
GLU
A
279
26.928
33.691
24.766
1.00
13.94
O


ATOM
3954
C
GLU
A
279
27.387
29.439
25.978
1.00
10.50
C


ATOM
3955
O
GLU
A
279
26.477
30.093
26.462
1.00
10.16
O


ATOM
3956
N
LEU
A
280
27.198
28.205
25.499
1.00
10.73
N


ATOM
3958
CA
LEU
A
280
25.898
27.507
25.652
1.00
10.31
C


ATOM
3960
CB
LEU
A
280
25.846
26.124
24.973
1.00
12.52
C


ATOM
3963
CG
LEU
A
280
25.772
26.107
23.451
1.00
13.85
C


ATOM
3965
CD1
LEU
A
280
25.964
24.631
22.911
1.00
17.63
C


ATOM
3969
CD2
LEU
A
280
24.435
26.669
22.957
1.00
15.10
C


ATOM
3973
C
LEU
A
280
25.613
27.411
27.150
1.00
10.89
C


ATOM
3974
O
LEU
A
280
24.492
27.626
27.606
1.00
12.00
O


ATOM
3975
N
GLN
A
281
26.630
27.040
27.907
1.00
9.57
N


ATOM
3977
CA
GLN
A
281
26.479
26.931
29.322
1.00
11.52
C


ATOM
3979
CB
GLN
A
281
27.755
26.360
29.925
1.00
11.03
C


ATOM
3982
CG
GLN
A
281
28.028
24.891
29.487
1.00
12.22
C


ATOM
3985
CD
GLN
A
281
29.376
24.377
29.981
1.00
14.98
C


ATOM
3986
OE1
GLN
A
281
30.115
25.172
30.555
1.00
15.61
O


ATOM
3987
NE2
GLN
A
281
29.731
23.103
29.697
1.00
9.95
N


ATOM
3990
C
GLN
A
281
26.161
28.313
29.924
1.00
10.71
C


ATOM
3991
O
GLN
A
281
25.309
28.409
30.815
1.00
11.77
O


ATOM
3992
N
ASN
A
282
26.883
29.367
29.484
1.00
10.78
N


ATOM
3994
CA
ASN
A
282
26.722
30.715
30.090
1.00
10.24
C


ATOM
3996
CB
ASN
A
282
27.683
31.745
29.504
1.00
10.08
C


ATOM
3999
CG
ASN
A
282
29.136
31.482
29.812
1.00
13.02
C


ATOM
4000
OD1
ASN
A
282
29.471
30.830
30.809
1.00
14.49
O


ATOM
4001
ND2
ASN
A
282
30.003
32.000
28.973
1.00
12.29
N


ATOM
4004
C
ASN
A
282
25.275
31.172
29.788
1.00
11.08
C


ATOM
4005
O
ASN
A
282
24.588
31.667
30.681
1.00
12.10
O


ATOM
4006
N
ARG
A
283
24.829
30.902
28.575
1.00
9.30
N


ATOM
4008
CA
ARG
A
283
23.484
31.298
28.160
1.00
10.89
C


ATOM
4010
CB
ARG
A
283
23.305
31.055
26.697
1.00
10.96
C


ATOM
4013
CG
ARG
A
283
23.894
32.191
25.842
1.00
13.15
C


ATOM
4016
CD
ARG
A
283
23.768
31.880
24.383
1.00
11.91
C


ATOM
4019
NE
ARG
A
283
24.469
32.831
23.466
1.00
12.89
N


ATOM
4021
CZ
ARG
A
283
23.985
33.290
22.311
1.00
15.41
C


ATOM
4022
NH1
ARG
A
283
22.780
33.006
21.903
1.00
15.22
N


ATOM
4025
NH2
ARG
A
283
24.722
34.053
21.528
1.00
14.46
N


ATOM
4028
C
ARG
A
283
22.450
30.528
28.963
1.00
10.28
C


ATOM
4029
O
ARG
A
283
21.390
31.063
29.298
1.00
11.49
O


ATOM
4030
N
ALA
A
284
22.741
29.263
29.220
1.00
10.27
N


ATOM
4032
CA
ALA
A
284
21.789
28.438
29.957
1.00
10.07
C


ATOM
4034
CB
ALA
A
284
22.358
27.011
30.158
1.00
11.06
C


ATOM
4038
C
ALA
A
284
21.493
29.093
31.306
1.00
10.53
C


ATOM
4039
O
ALA
A
284
20.349
29.096
31.809
1.00
11.23
O


ATOM
4040
N
LYS
A
285
22.539
29.620
31.899
1.00
10.42
N


ATOM
4042
CA
LYS
A
285
22.446
30.158
33.238
1.00
10.99
C


ATOM
4044
CB
LYS
A
285
23.846
30.372
33.821
1.00
11.62
C


ATOM
4047
CG
LYS
A
285
24.664
29.120
34.054
1.00
12.18
C


ATOM
4050
CD
LYS
A
285
26.057
29.529
34.477
1.00
16.21
C


ATOM
4053
CE
LYS
A
285
27.062
28.492
34.451
1.00
15.01
C


ATOM
4056
NZ
LYS
A
285
28.349
29.067
35.077
1.00
17.35
N


ATOM
4060
C
LYS
A
285
21.566
31.386
33.281
1.00
11.59
C


ATOM
4061
O
LYS
A
285
21.120
31.753
34.363
1.00
12.70
O


ATOM
4062
N
VAL
A
286
21.419
32.094
32.168
1.00
10.88
N


ATOM
4064
CA
VAL
A
286
20.604
33.255
32.110
1.00
12.69
C


ATOM
4066
CB
VAL
A
286
20.719
33.961
30.788
1.00
12.21
C


ATOM
4068
CG1
VAL
A
286
19.838
35.220
30.746
1.00
15.12
C


ATOM
4072
CG2
VAL
A
286
22.221
34.402
30.612
1.00
13.05
C


ATOM
4076
C
VAL
A
286
19.153
32.850
32.362
1.00
13.03
C


ATOM
4077
O
VAL
A
286
18.399
33.651
32.960
1.00
13.04
O


ATOM
4078
N
TYR
A
287
18.788
31.649
31.885
1.00
11.44
N


ATOM
4080
CA
TYR
A
287
17.427
31.124
32.057
1.00
12.02
C


ATOM
4082
CB
TYR
A
287
16.740
30.949
30.718
1.00
12.84
C


ATOM
4085
CG
TYR
A
287
16.587
32.235
29.976
1.00
12.74
C


ATOM
4086
CD1
TYR
A
287
15.615
33.157
30.344
1.00
14.60
C


ATOM
4088
CE1
TYR
A
287
15.490
34.343
29.748
1.00
14.77
C


ATOM
4090
CZ
TYR
A
287
16.343
34.709
28.753
1.00
15.62
C


ATOM
4091
OH
TYR
A
287
16.139
35.938
28.183
1.00
18.73
O


ATOM
4093
CE2
TYR
A
287
17.372
33.852
28.377
1.00
13.12
C


ATOM
4095
CD2
TYR
A
287
17.521
32.646
28.995
1.00
11.74
C


ATOM
4097
C
TYR
A
287
17.427
29.860
32.905
1.00
12.13
C


ATOM
4098
O
TYR
A
287
17.303
28.740
32.452
1.00
13.99
O


ATOM
4099
N
ASP
A
288
17.631
30.057
34.208
1.00
12.75
N


ATOM
4101
CA
ASP
A
288
17.553
29.009
35.170
1.00
11.58
C


ATOM
4103
CB
ASP
A
288
17.721
29.636
36.551
1.00
11.65
C


ATOM
4106
CG
ASP
A
288
17.912
28.612
37.687
1.00
15.60
C


ATOM
4107
OD1
ASP
A
288
18.065
27.373
37.473
1.00
17.35
O


ATOM
4108
OD2
ASP
A
288
17.934
29.001
38.898
1.00
15.75
O


ATOM
4109
C
ASP
A
288
16.174
28.337
35.056
1.00
12.45
C


ATOM
4110
O
ASP
A
288
15.186
29.032
34.950
1.00
13.43
O


ATOM
4111
N
ILE
A
289
16.112
27.026
34.986
1.00
10.72
N


ATOM
4113
CA
ILE
A
289
14.812
26.271
34.969
1.00
12.80
C


ATOM
4115
CB
ILE
A
289
14.860
25.168
33.912
1.00
12.78
C


ATOM
4117
CG1
ILE
A
289
15.042
25.785
32.535
1.00
13.67
C


ATOM
4120
CD1
ILE
A
289
13.745
26.474
31.867
1.00
13.36
C


ATOM
4124
CG2
ILE
A
289
13.659
24.220
33.989
1.00
14.37
C


ATOM
4128
C
ILE
A
289
14.517
25.742
36.352
1.00
13.79
C


ATOM
4129
O
ILE
A
289
15.376
25.140
37.045
1.00
12.95
O


ATOM
4130
N
LYS
A
290
13.299
26.006
36.825
1.00
14.91
N


ATOM
4132
CA
LYS
A
290
12.904
25.675
38.185
1.00
16.59
C


ATOM
4134
CB
LYS
A
290
12.600
26.955
38.930
1.00
17.67
C


ATOM
4137
CG
LYS
A
290
13.878
27.786
39.143
1.00
21.86
C


ATOM
4140
CD
LYS
A
290
13.627
28.893
40.134
1.00
28.07
C


ATOM
4143
CE
LYS
A
290
13.554
30.215
39.470
1.00
34.10
C


ATOM
4146
NZ
LYS
A
290
13.272
31.317
40.512
1.00
39.60
N


ATOM
4150
C
LYS
A
290
11.648
24.807
38.235
1.00
16.71
C


ATOM
4151
O
LYS
A
290
11.200
24.444
39.330
1.00
18.64
O


ATOM
4152
N
GLY
A
291
11.119
24.507
37.071
1.00
15.39
N


ATOM
4154
CA
GLY
A
291
9.895
23.740
36.907
1.00
14.24
C


ATOM
4157
C
GLY
A
291
10.134
22.294
36.570
1.00
14.85
C


ATOM
4158
O
GLY
A
291
10.997
21.990
35.707
1.00
15.05
O


ATOM
4159
N
GLY
A
292
9.376
21.387
37.185
1.00
15.54
N


ATOM
4161
CA
GLY
A
292
9.524
19.984
36.896
1.00
14.12
C


ATOM
4164
C
GLY
A
292
10.116
19.187
38.030
1.00
15.07
C


ATOM
4165
O
GLY
A
292
10.717
19.734
38.959
1.00
15.42
O


ATOM
4166
N
ILE
A
293
9.932
17.878
37.980
1.00
14.74
N


ATOM
4168
CA
ILE
A
293
10.363
17.018
39.044
1.00
15.41
C


ATOM
4170
CB
ILE
A
293
9.881
15.599
38.776
1.00
15.32
C


ATOM
4172
CG1
ILE
A
293
8.342
15.549
38.894
1.00
18.82
C


ATOM
4175
CD1
ILE
A
293
7.731
14.260
38.471
1.00
21.20
C


ATOM
4179
CG2
ILE
A
293
10.526
14.610
39.740
1.00
15.00
C


ATOM
4183
C
ILE
A
293
11.885
17.052
39.176
1.00
16.26
C


ATOM
4184
O
ILE
A
293
12.586
16.688
38.214
1.00
14.62
O


ATOM
4185
N
GLY
A
294
12.367
17.439
40.346
1.00
14.72
N


ATOM
4187
CA
GLY
A
294
13.810
17.581
40.559
1.00
15.36
C


ATOM
4190
C
GLY
A
294
14.449
18.897
40.107
1.00
13.63
C


ATOM
4191
O
GLY
A
294
15.660
19.095
40.315
1.00
15.06
O


ATOM
4192
N
ALA
A
295
13.688
19.803
39.516
1.00
14.00
N


ATOM
4194
CA
ALA
A
295
14.220
21.064
39.058
1.00
13.92
C


ATOM
4196
CB
ALA
A
295
13.381
21.661
37.869
1.00
14.66
C


ATOM
4200
C
ALA
A
295
14.226
21.990
40.236
1.00
16.73
C


ATOM
4201
O
ALA
A
295
13.451
21.811
41.178
1.00
16.12
O


ATOM
4202
N
GLY
A
296
15.094
22.986
40.227
1.00
16.79
N


ATOM
4204
CA
GLY
A
296
15.114
23.941
41.340
1.00
18.72
C


ATOM
4207
C
GLY
A
296
16.052
25.058
41.052
1.00
17.66
C


ATOM
4208
O
GLY
A
296
16.601
25.160
39.963
1.00
16.21
O


ATOM
4209
N
THR
A
297
16.240
25.979
41.992
1.00
17.88
N


ATOM
4211
CA
THR
A
297
17.144
27.068
41.712
1.00
17.28
C


ATOM
4213
CB
THR
A
297
17.117
27.981
42.941
1.00
19.91
C


ATOM
4215
OG1
THR
A
297
15.763
28.455
43.102
1.00
19.89
O


ATOM
4217
CG2
THR
A
297
17.981
29.177
42.689
1.00
19.92
C


ATOM
4221
C
THR
A
297
18.563
26.600
41.472
1.00
17.13
C


ATOM
4222
O
THR
A
297
19.104
25.832
42.248
1.00
18.37
O


ATOM
4223
N
GLY
A
298
19.193
27.074
40.410
1.00
14.91
N


ATOM
4225
CA
GLY
A
298
20.569
26.702
40.153
1.00
15.10
C


ATOM
4228
C
GLY
A
298
20.684
25.492
39.273
1.00
14.01
C


ATOM
4229
O
GLY
A
298
19.717
25.039
38.656
1.00
12.62
O


ATOM
4230
N
ASP
A
299
21.882
24.932
39.243
1.00
13.64
N


ATOM
4232
CA
ASP
A
299
22.189
23.741
38.460
1.00
13.68
C


ATOM
4234
CB
ASP
A
299
23.689
23.623
38.447
1.00
14.28
C


ATOM
4237
CG
ASP
A
299
24.229
22.342
37.843
1.00
15.32
C


ATOM
4238
OD1
ASP
A
299
23.578
21.787
36.970
1.00
13.87
O


ATOM
4239
OD2
ASP
A
299
25.330
21.885
38.238
1.00
13.70
O


ATOM
4240
C
ASP
A
299
21.507
22.602
39.092
1.00
14.87
C


ATOM
4241
O
ASP
A
299
21.657
22.381
40.306
1.00
16.32
O


ATOM
4242
N
ASP
A
300
20.664
21.901
38.348
1.00
12.69
N


ATOM
4244
CA
ASP
A
300
19.921
20.812
38.953
1.00
12.30
C


ATOM
4246
CB
ASP
A
300
18.489
21.282
39.369
1.00
12.84
C


ATOM
4249
CG
ASP
A
300
17.615
21.597
38.192
1.00
11.72
C


ATOM
4250
OD1
ASP
A
300
17.330
20.687
37.428
1.00
13.65
O


ATOM
4251
OD2
ASP
A
300
17.079
22.691
38.018
1.00
14.33
O


ATOM
4252
C
ASP
A
300
19.928
19.603
38.011
1.00
13.91
C


ATOM
4253
O
ASP
A
300
20.278
19.699
36.822
1.00
13.63
O


ATOM
4254
N
TYR
A
301
19.514
18.462
38.539
1.00
13.49
N


ATOM
4256
CA
TYR
A
301
19.627
17.220
37.803
1.00
12.93
C


ATOM
4258
CB
TYR
A
301
19.868
16.046
38.777
1.00
11.22
C


ATOM
4261
CG
TYR
A
301
18.804
15.846
39.823
1.00
13.88
C


ATOM
4262
CD1
TYR
A
301
17.648
15.225
39.523
1.00
14.91
C


ATOM
4264
CE1
TYR
A
301
16.621
15.079
40.531
1.00
17.24
C


ATOM
4266
CZ
TYR
A
301
16.809
15.580
41.788
1.00
19.26
C


ATOM
4267
OH
TYR
A
301
15.803
15.394
42.776
1.00
20.79
O


ATOM
4269
CE2
TYR
A
301
17.977
16.242
42.081
1.00
16.95
C


ATOM
4271
CD2
TYR
A
301
18.948
16.384
41.095
1.00
13.89
C


ATOM
4273
C
TYR
A
301
18.421
16.951
36.875
1.00
11.64
C


ATOM
4274
O
TYR
A
301
18.419
15.954
36.154
1.00
11.11
O


ATOM
4275
N
ALA
A
302
17.435
17.846
36.832
1.00
11.29
N


ATOM
4277
CA
ALA
A
302
16.389
17.756
35.829
1.00
10.84
C


ATOM
4279
CB
ALA
A
302
15.006
18.227
36.391
1.00
12.99
C


ATOM
4283
C
ALA
A
302
16.684
18.574
34.581
1.00
10.79
C


ATOM
4284
O
ALA
A
302
16.424
18.146
33.444
1.00
11.72
O


ATOM
4285
N
SER
A
303
17.194
19.774
34.790
1.00
10.48
N


ATOM
4287
CA
SER
A
303
17.364
20.706
33.697
1.00
9.41
C


ATOM
4289
CB
SER
A
303
16.512
21.934
33.969
1.00
9.70
C


ATOM
4292
OG
SER
A
303
16.992
22.660
35.130
1.00
12.05
O


ATOM
4294
C
SER
A
303
18.819
21.172
33.468
1.00
10.16
C


ATOM
4295
O
SER
A
303
19.049
21.949
32.566
1.00
10.53
O


ATOM
4296
N
GLY
A
304
19.742
20.739
34.303
1.00
8.73
N


ATOM
4298
CA
GLY
A
304
21.132
21.139
34.201
1.00
10.18
C


ATOM
4301
C
GLY
A
304
21.273
22.616
34.479
1.00
10.17
C


ATOM
4302
O
GLY
A
304
20.710
23.138
35.422
1.00
10.02
O


ATOM
4303
N
PHE
A
305
22.102
23.286
33.664
1.00
11.73
N


ATOM
4305
CA
PHE
A
305
22.452
24.644
33.880
1.00
10.68
C


ATOM
4307
CB
PHE
A
305
23.661
25.048
33.036
1.00
10.84
C


ATOM
4310
CG
PHE
A
305
24.977
24.418
33.419
1.00
11.70
C


ATOM
4311
CD1
PHE
A
305
25.070
23.280
34.195
1.00
12.20
C


ATOM
4313
CE1
PHE
A
305
26.316
22.689
34.511
1.00
12.74
C


ATOM
4315
CZ
PHE
A
305
27.499
23.265
33.973
1.00
11.93
C


ATOM
4317
CE2
PHE
A
305
27.380
24.372
33.184
1.00
11.62
C


ATOM
4319
CD2
PHE
A
305
26.157
24.941
32.891
1.00
12.26
C


ATOM
4321
C
PHE
A
305
21.349
25.614
33.554
1.00
11.33
C


ATOM
4322
O
PHE
A
305
21.368
26.748
34.022
1.00
11.39
O


ATOM
4323
N
GLY
A
306
20.364
25.161
32.811
1.00
10.40
N


ATOM
4325
CA
GLY
A
306
19.276
26.018
32.414
1.00
10.88
C


ATOM
4328
C
GLY
A
306
18.926
25.834
30.963
1.00
11.20
C


ATOM
4329
O
GLY
A
306
19.208
24.812
30.318
1.00
9.40
O


ATOM
4330
N
TYR
A
307
18.291
26.863
30.443
1.00
10.29
N


ATOM
4332
CA
TYR
A
307
17.803
26.905
29.077
1.00
11.10
C


ATOM
4334
CB
TYR
A
307
16.311
27.194
29.162
1.00
10.85
C


ATOM
4337
CG
TYR
A
307
15.557
27.309
27.870
1.00
10.79
C


ATOM
4338
CD1
TYR
A
307
16.087
26.885
26.660
1.00
10.35
C


ATOM
4340
CE1
TYR
A
307
15.374
27.001
25.479
1.00
10.97
C


ATOM
4342
CZ
TYR
A
307
14.086
27.458
25.503
1.00
13.85
C


ATOM
4343
OH
TYR
A
307
13.388
27.607
24.308
1.00
13.18
O


ATOM
4345
CE2
TYR
A
307
13.531
27.912
26.705
1.00
13.38
C


ATOM
4347
CD2
TYR
A
307
14.260
27.849
27.860
1.00
11.68
C


ATOM
4349
C
TYR
A
307
18.479
27.939
28.217
1.00
12.67
C


ATOM
4350
O
TYR
A
307
18.140
29.113
28.271
1.00
13.67
O


ATOM
4351
N
PRO
A
308
19.440
27.555
27.389
1.00
11.31
N


ATOM
4352
CA
PRO
A
308
20.118
28.547
26.531
1.00
11.31
C


ATOM
4354
CB
PRO
A
308
21.315
27.780
25.971
1.00
10.82
C


ATOM
4357
CG
PRO
A
308
20.941
26.293
26.070
1.00
11.80
C


ATOM
4360
CD
PRO
A
308
19.833
26.179
27.077
1.00
12.92
C


ATOM
4363
C
PRO
A
308
19.245
29.003
25.361
1.00
11.34
C


ATOM
4364
O
PRO
A
308
18.604
28.159
24.689
1.00
10.74
O


ATOM
4365
N
ARG
A
309
19.314
30.266
25.051
1.00
12.86
N


ATOM
4367
CA
ARG
A
309
18.427
30.864
24.076
1.00
13.91
C


ATOM
4369
CB
ARG
A
309
17.252
31.527
24.844
1.00
13.02
C


ATOM
4372
CG
ARG
A
309
16.469
30.638
25.781
1.00
13.54
C


ATOM
4375
CD
ARG
A
309
15.375
31.251
26.661
1.00
14.50
C


ATOM
4378
NE
ARG
A
309
14.099
31.449
25.978
1.00
15.52
N


ATOM
4380
CZ
ARG
A
309
13.022
31.993
26.525
1.00
16.18
C


ATOM
4381
NH1
ARG
A
309
13.055
32.423
27.777
1.00
12.53
N


ATOM
4384
NH2
ARG
A
309
11.891
32.006
25.832
1.00
15.18
N


ATOM
4387
C
ARG
A
309
19.139
31.870
23.213
1.00
14.57
C


ATOM
4388
O
ARG
A
309
20.288
32.256
23.468
1.00
13.36
O


ATOM
4389
N
VAL
A
310
18.455
32.367
22.180
1.00
13.70
N


ATOM
4391
CA
VAL
A
310
19.053
33.390
21.325
1.00
15.05
C


ATOM
4393
CB
VAL
A
310
18.134
33.596
20.101
1.00
14.71
C


ATOM
4395
CG1
VAL
A
310
18.580
34.793
19.258
1.00
18.54
C


ATOM
4399
CG2
VAL
A
310
18.121
32.368
19.265
1.00
14.81
C


ATOM
4403
C
VAL
A
310
19.272
34.671
22.112
1.00
16.45
C


ATOM
4404
O
VAL
A
310
20.251
35.382
21.905
1.00
15.49
O


ATOM
4405
N
LYS
A
311
18.316
34.979
22.979
1.00
17.77
N


ATOM
4407
CA
LYS
A
311
18.415
36.106
23.940
1.00
18.52
C


ATOM
4409
CB
LYS
A
311
17.942
37.380
23.244
1.00
18.11
C


ATOM
4412
CG
LYS
A
311
16.677
37.137
22.440
1.00
23.20
C


ATOM
4415
CD
LYS
A
311
15.574
38.112
22.630
1.00
33.02
C


ATOM
4418
CE
LYS
A
311
15.667
39.252
21.732
1.00
34.63
C


ATOM
4421
NZ
LYS
A
311
14.289
39.716
21.277
1.00
36.21
N


ATOM
4425
C
LYS
A
311
17.532
35.897
25.178
1.00
17.62
C


ATOM
4426
O
LYS
A
311
16.770
34.912
25.199
1.00
16.50
O


ATOM
4427
OXT
LYS
A
311
17.534
36.644
26.172
1.00
19.57
O


ATOM
4428
CA
CA
C
312
28.232
18.547
38.069
1.00
13.89
CA


ATOM
4429
CA
CA
C
313
17.608
25.091
37.856
1.00
18.60
CA


ATOM
4430
CA
CA
C
314
27.338
14.925
29.013
0.60
10.25
CA


ATOM
4431
N
ALA
B
318
2.727
2.475
36.156
1.00
30.60
N


ATOM
4433
CA
ALA
B
318
2.319
3.152
34.902
1.00
28.10
C


ATOM
4435
CB
ALA
B
318
1.428
4.295
35.200
1.00
27.75
C


ATOM
4439
C
ALA
B
318
3.422
3.533
33.900
1.00
24.85
C


ATOM
4440
O
ALA
B
318
3.103
3.596
32.739
1.00
27.46
O


ATOM
4443
N
THR
B
319
4.625
3.965
34.273
1.00
24.93
N


ATOM
4445
CA
THR
B
319
5.658
4.224
33.231
1.00
23.26
C


ATOM
4447
CB
THR
B
319
6.154
5.690
33.296
1.00
24.03
C


ATOM
4449
OG1
THR
B
319
6.811
5.953
34.535
1.00
27.37
O


ATOM
4451
CG2
THR
B
319
4.960
6.670
33.258
1.00
27.35
C


ATOM
4455
C
THR
B
319
6.926
3.305
33.235
1.00
22.75
C


ATOM
4456
O
THR
B
319
7.820
3.498
32.406
1.00
21.26
O


ATOM
4457
N
GLU
B
320
7.027
2.401
34.205
1.00
21.07
N


ATOM
4459
CA
GLU
B
320
8.177
1.559
34.324
1.00
21.49
C


ATOM
4461
CB
GLU
B
320
9.328
2.280
35.014
1.00
21.67
C


ATOM
4464
CG
GLU
B
320
8.980
2.681
36.413
1.00
25.74
C


ATOM
4467
CD
GLU
B
320
10.174
3.130
37.222
1.00
34.05
C


ATOM
4468
OE1
GLU
B
320
10.962
3.910
36.698
1.00
36.87
O


ATOM
4469
OE2
GLU
B
320
10.295
2.711
38.394
1.00
39.11
O


ATOM
4470
C
GLU
B
320
7.818
0.308
35.082
1.00
20.20
C


ATOM
4471
O
GLU
B
320
6.914
0.330
35.945
1.00
20.85
O


ATOM
4472
N
TRP
B
321
8.526
−0.774
34.748
1.00
18.06
N


ATOM
4474
CA
TRP
B
321
8.271
−2.092
35.310
1.00
17.54
C


ATOM
4476
CB
TRP
B
321
7.595
−2.961
34.277
1.00
15.93
C


ATOM
4479
CG
TRP
B
321
6.265
−2.537
33.906
1.00
18.41
C


ATOM
4480
CD1
TRP
B
321
5.089
−3.007
34.445
1.00
16.53
C


ATOM
4482
NE1
TRP
B
321
4.017
−2.406
33.836
1.00
19.73
N


ATOM
4484
CE2
TRP
B
321
4.470
−1.523
32.886
1.00
15.64
C


ATOM
4485
CD2
TRP
B
321
5.889
−1.576
32.903
1.00
15.37
C


ATOM
4486
CE3
TRP
B
321
6.596
−0.772
31.992
1.00
14.76
C


ATOM
4488
CZ3
TRP
B
321
5.852
0.097
31.140
1.00
17.06
C


ATOM
4490
CH2
TRP
B
321
4.428
0.089
31.165
1.00
17.68
C


ATOM
4492
CZ2
TRP
B
321
3.757
−0.693
32.047
1.00
18.99
C


ATOM
4494
C
TRP
B
321
9.570
−2.716
35.728
1.00
16.82
C


ATOM
4495
O
TRP
B
321
10.068
−3.654
35.131
1.00
16.09
O


ATOM
4496
N
PRO
B
322
10.186
−2.151
36.755
1.00
18.33
N


ATOM
4497
CA
PRO
B
322
11.493
−2.628
37.202
1.00
19.01
C


ATOM
4499
CB
PRO
B
322
11.829
−1.725
38.392
1.00
19.99
C


ATOM
4502
CG
PRO
B
322
10.556
−1.019
38.744
1.00
19.55
C


ATOM
4505
CD
PRO
B
322
9.651
−1.061
37.575
1.00
19.07
C


ATOM
4508
C
PRO
B
322
11.484
−4.065
37.641
1.00
19.86
C


ATOM
4509
O
PRO
B
322
12.495
−4.748
37.546
1.00
20.25
O


ATOM
4510
N
GLU
B
323
10.334
−4.557
38.050
1.00
20.04
N


ATOM
4512
CA
GLU
B
323
10.262
−5.906
38.546
1.00
20.93
C


ATOM
4514
CB
GLU
B
323
8.960
−6.041
39.331
1.00
23.24
C


ATOM
4517
CG
GLU
B
323
7.708
−5.954
38.441
1.00
26.81
C


ATOM
4520
CD
GLU
B
323
7.184
−4.530
38.089
1.00
27.51
C


ATOM
4521
OE1
GLU
B
323
7.879
−3.511
38.275
1.00
20.70
O


ATOM
4522
OE2
GLU
B
323
5.996
−4.461
37.641
1.00
30.50
O


ATOM
4523
C
GLU
B
323
10.325
−6.934
37.407
1.00
18.55
C


ATOM
4524
O
GLU
B
323
10.521
−8.111
37.642
1.00
16.50
O


ATOM
4525
N
LEU
B
324
10.256
−6.485
36.172
1.00
16.08
N


ATOM
4527
CA
LEU
B
324
10.357
−7.398
35.050
1.00
15.61
C


ATOM
4529
CB
LEU
B
324
9.626
−6.849
33.846
1.00
15.54
C


ATOM
4532
CG
LEU
B
324
8.113
−6.779
34.039
1.00
16.64
C


ATOM
4534
CD1
LEU
B
324
7.437
−6.061
32.923
1.00
19.42
C


ATOM
4538
CD2
LEU
B
324
7.648
−8.212
34.152
1.00
19.22
C


ATOM
4542
C
LEU
B
324
11.815
−7.755
34.643
1.00
14.32
C


ATOM
4543
O
LEU
B
324
12.017
−8.619
33.836
1.00
14.01
O


ATOM
4544
N
VAL
B
325
12.792
−7.073
35.189
1.00
15.66
N


ATOM
4546
CA
VAL
B
325
14.180
−7.370
34.816
1.00
15.56
C


ATOM
4548
CB
VAL
B
325
15.184
−6.410
35.456
1.00
15.71
C


ATOM
4550
CG1
VAL
B
325
16.629
−6.860
35.094
1.00
16.62
C


ATOM
4554
CG2
VAL
B
325
14.953
−4.946
34.988
1.00
14.67
C


ATOM
4558
C
VAL
B
325
14.478
−8.817
35.197
1.00
15.70
C


ATOM
4559
O
VAL
B
325
14.181
−9.219
36.316
1.00
15.01
O


ATOM
4560
N
GLY
B
326
14.985
−9.609
34.247
1.00
16.26
N


ATOM
4562
CA
GLY
B
326
15.302
−11.008
34.494
1.00
15.67
C


ATOM
4565
C
GLY
B
326
14.166
−11.958
34.140
1.00
16.74
C


ATOM
4566
O
GLY
B
326
14.358
−13.159
34.108
1.00
16.04
O


ATOM
4567
N
LYS
B
327
12.957
−11.432
33.950
1.00
16.30
N


ATOM
4569
CA
LYS
B
327
11.848
−12.266
33.510
1.00
17.25
C


ATOM
4571
CB
LYS
B
327
10.514
−11.632
33.958
1.00
17.70
C


ATOM
4574
CG
LYS
B
327
10.573
−11.307
35.417
1.00
22.70
C


ATOM
4577
CD
LYS
B
327
9.217
−11.378
36.140
1.00
30.58
C


ATOM
4580
CE
LYS
B
327
9.404
−11.302
37.674
1.00
31.61
C


ATOM
4583
NZ
LYS
B
327
10.674
−11.962
38.027
1.00
33.63
N


ATOM
4587
C
LYS
B
327
11.837
−12.497
32.021
1.00
16.69
C


ATOM
4588
O
LYS
B
327
12.479
−11.805
31.222
1.00
15.76
O


ATOM
4589
N
SER
B
328
11.055
−13.485
31.601
1.00
18.24
N


ATOM
4591
CA
SER
B
328
10.972
−13.809
30.188
1.00
17.59
C


ATOM
4593
CB
SER
B
328
10.280
−15.154
29.994
1.00
19.45
C


ATOM
4596
OG
SER
B
328
8.887
−15.026
30.192
1.00
18.39
O


ATOM
4598
C
SER
B
328
10.206
−12.757
29.431
1.00
18.63
C


ATOM
4599
O
SER
B
328
9.375
−12.020
30.003
1.00
17.45
O


ATOM
4600
N
VAL
B
329
10.494
−12.653
28.140
1.00
18.81
N


ATOM
4602
CA
VAL
B
329
9.771
−11.690
27.299
1.00
20.52
C


ATOM
4604
CB
VAL
B
329
10.317
−11.663
25.852
1.00
21.15
C


ATOM
4606
CG1
VAL
B
329
9.817
−12.809
25.060
1.00
22.84
C


ATOM
4610
CG2
VAL
B
329
9.914
−10.411
25.159
1.00
25.35
C


ATOM
4614
C
VAL
B
329
8.276
−11.962
27.279
1.00
20.54
C


ATOM
4615
O
VAL
B
329
7.460
−11.049
27.237
1.00
19.76
O


ATOM
4616
N
GLU
B
330
7.906
−13.228
27.332
1.00
21.77
N


ATOM
4618
CA
GLU
B
330
6.504
−13.544
27.379
1.00
22.29
C


ATOM
4620
CB
GLU
B
330
6.331
−15.048
27.153
1.00
24.60
C


ATOM
4623
CG
GLU
B
330
6.850
−15.472
25.754
1.00
27.68
C


ATOM
4626
CD
GLU
B
330
8.341
−15.854
25.674
1.00
35.11
C


ATOM
4627
OE1
GLU
B
330
9.141
−15.647
26.617
1.00
28.88
O


ATOM
4628
OE2
GLU
B
330
8.722
−16.426
24.632
1.00
41.94
O


ATOM
4629
C
GLU
B
330
5.809
−13.084
28.642
1.00
22.11
C


ATOM
4630
O
GLU
B
330
4.676
−12.586
28.584
1.00
21.27
O


ATOM
4631
N
GLU
B
331
6.452
−13.239
29.790
1.00
21.03
N


ATOM
4633
CA
GLU
B
331
5.904
−12.751
31.043
1.00
21.48
C


ATOM
4635
CB
GLU
B
331
6.730
−13.205
32.262
1.00
22.76
C


ATOM
4638
CG
GLU
B
331
5.844
−13.545
33.454
1.00
31.85
C


ATOM
4641
CD
GLU
B
331
6.490
−13.408
34.816
1.00
38.17
C


ATOM
4642
OE1
GLU
B
331
7.584
−14.024
35.014
1.00
46.35
O


ATOM
4643
OE2
GLU
B
331
5.886
−12.712
35.693
1.00
37.37
O


ATOM
4644
C
GLU
B
331
5.838
−11.243
31.046
1.00
19.51
C


ATOM
4645
O
GLU
B
331
4.892
−10.668
31.537
1.00
18.89
O


ATOM
4646
N
ALA
B
332
6.882
−10.609
30.514
1.00
18.18
N


ATOM
4648
CA
ALA
B
332
6.951
−9.187
30.538
1.00
15.95
C


ATOM
4650
CB
ALA
B
332
8.254
−8.709
29.931
1.00
16.83
C


ATOM
4654
C
ALA
B
332
5.801
−8.640
29.757
1.00
15.42
C


ATOM
4655
O
ALA
B
332
5.163
−7.697
30.182
1.00
14.99
O


ATOM
4656
N
LYS
B
333
5.580
−9.189
28.589
1.00
15.39
N


ATOM
4658
CA
LYS
B
333
4.489
−8.693
27.748
1.00
17.34
C


ATOM
4660
CB
LYS
B
333
4.458
−9.450
26.441
1.00
17.67
C


ATOM
4663
CG
LYS
B
333
5.438
−9.004
25.401
1.00
21.32
C


ATOM
4666
CD
LYS
B
333
5.200
−9.807
24.128
1.00
23.42
C


ATOM
4669
CE
LYS
B
333
6.357
−9.619
23.113
1.00
28.93
C


ATOM
4672
NZ
LYS
B
333
6.005
−10.290
21.820
1.00
28.19
N


ATOM
4676
C
LYS
B
333
3.127
−8.789
28.452
1.00
17.99
C


ATOM
4677
O
LYS
B
333
2.338
−7.868
28.410
1.00
15.95
O


ATOM
4678
N
LYS
B
334
2.858
−9.904
29.125
1.00
19.30
N


ATOM
4680
CA
LYS
B
334
1.595
−10.046
29.842
1.00
19.86
C


ATOM
4682
CB
LYS
B
334
1.417
−11.432
30.481
1.00
21.24
C


ATOM
4685
CG
LYS
B
334
0.949
−12.476
29.568
1.00
28.52
C


ATOM
4688
CD
LYS
B
334
0.499
−13.819
30.317
1.00
33.48
C


ATOM
4691
CE
LYS
B
334
0.650
−15.022
29.319
1.00
37.14
C


ATOM
4694
NZ
LYS
B
334
0.870
−14.604
27.850
1.00
36.97
N


ATOM
4698
C
LYS
B
334
1.457
−8.983
30.890
1.00
18.97
C


ATOM
4699
O
LYS
B
334
0.386
−8.343
31.021
1.00
16.86
O


ATOM
4700
N
VAL
B
335
2.522
−8.754
31.658
1.00
16.84
N


ATOM
4702
CA
VAL
B
335
2.480
−7.783
32.726
1.00
16.65
C


ATOM
4704
CB
VAL
B
335
3.743
−7.823
33.591
1.00
15.76
C


ATOM
4706
CG1
VAL
B
335
3.817
−6.655
34.612
1.00
19.24
C


ATOM
4710
CG2
VAL
B
335
3.789
−9.108
34.341
1.00
17.38
C


ATOM
4714
C
VAL
B
335
2.288
−6.382
32.203
1.00
15.76
C


ATOM
4715
O
VAL
B
335
1.494
−5.615
32.763
1.00
16.18
O


ATOM
4716
N
ILE
B
336
3.033
−6.023
31.167
1.00
15.08
N


ATOM
4718
CA
ILE
B
336
2.903
−4.703
30.635
1.00
14.79
C


ATOM
4720
CB
ILE
B
336
3.967
−4.441
29.592
1.00
14.82
C


ATOM
4722
CG1
ILE
B
336
5.290
−4.318
30.367
1.00
16.31
C


ATOM
4725
CD1
ILE
B
336
6.479
−4.602
29.579
1.00
19.31
C


ATOM
4729
CG2
ILE
B
336
3.543
−3.266
28.733
1.00
16.34
C


ATOM
4733
C
ILE
B
336
1.508
−4.472
30.074
1.00
15.10
C


ATOM
4734
O
ILE
B
336
0.914
−3.437
30.347
1.00
16.57
O


ATOM
4735
N
LEU
B
337
0.956
−5.456
29.390
1.00
15.14
N


ATOM
4737
CA
LEU
B
337
−0.343
−5.235
28.769
1.00
15.94
C


ATOM
4739
CB
LEU
B
337
−0.645
−6.290
27.727
1.00
15.77
C


ATOM
4742
CG
LEU
B
337
0.121
−6.195
26.404
1.00
13.58
C


ATOM
4744
CD1
LEU
B
337
0.021
−7.498
25.632
1.00
15.44
C


ATOM
4748
CD2
LEU
B
337
−0.350
−5.049
25.553
1.00
17.85
C


ATOM
4752
C
LEU
B
337
−1.450
−5.146
29.810
1.00
17.57
C


ATOM
4753
O
LEU
B
337
−2.511
−4.531
29.544
1.00
17.36
O


ATOM
4754
N
GLN
B
338
−1.210
−5.702
30.985
1.00
18.09
N


ATOM
4756
CA
GLN
B
338
−2.195
−5.610
32.047
1.00
21.31
C


ATOM
4758
CB
GLN
B
338
−1.830
−6.557
33.205
1.00
21.97
C


ATOM
4761
CG
GLN
B
338
−1.842
−8.050
32.775
1.00
26.01
C


ATOM
4764
CD
GLN
B
338
−1.562
−9.127
33.893
1.00
29.25
C


ATOM
4765
OE1
GLN
B
338
−0.720
−8.942
34.831
1.00
34.90
O


ATOM
4766
NE2
GLN
B
338
−2.211
−10.262
33.738
1.00
29.07
N


ATOM
4769
C
GLN
B
338
−2.398
−4.143
32.469
1.00
22.67
C


ATOM
4770
O
GLN
B
338
−3.530
−3.694
32.741
1.00
22.11
O


ATOM
4771
N
ASP
B
339
−1.318
−3.366
32.465
1.00
21.30
N


ATOM
4773
CA
ASP
B
339
−1.377
−1.936
32.761
1.00
21.98
C


ATOM
4775
CB
ASP
B
339
−0.047
−1.478
33.381
1.00
22.85
C


ATOM
4778
CG
ASP
B
339
0.213
−2.106
34.710
1.00
26.72
C


ATOM
4779
OD1
ASP
B
339
−0.740
−2.105
35.518
1.00
33.25
O


ATOM
4780
OD2
ASP
B
339
1.269
−2.680
35.026
1.00
26.47
O


ATOM
4781
C
ASP
B
339
−1.644
−1.066
31.555
1.00
22.06
C


ATOM
4782
O
ASP
B
339
−2.247
0.004
31.667
1.00
23.60
O


ATOM
4783
N
LYS
B
340
−1.218
−1.515
30.387
1.00
19.19
N


ATOM
4785
CA
LYS
B
340
−1.226
−0.722
29.218
1.00
18.98
C


ATOM
4787
CB
LYS
B
340
0.186
−0.070
29.118
1.00
18.88
C


ATOM
4790
CG
LYS
B
340
0.345
0.901
28.024
1.00
18.59
C


ATOM
4793
CD
LYS
B
340
1.805
1.491
28.014
1.00
19.17
C


ATOM
4796
CE
LYS
B
340
1.999
2.462
26.848
1.00
20.90
C


ATOM
4799
NZ
LYS
B
340
1.137
3.683
27.041
1.00
23.19
N


ATOM
4803
C
LYS
B
340
−1.611
−1.550
28.035
1.00
18.75
C


ATOM
4804
O
LYS
B
340
−0.822
−1.920
27.224
1.00
18.54
O


ATOM
4805
N
PRO
B
341
−2.906
−1.822
27.892
1.00
17.67
N


ATOM
4806
CA
PRO
B
341
−3.391
−2.768
26.911
1.00
17.68
C


ATOM
4808
CB
PRO
B
341
−4.926
−2.753
27.108
1.00
18.59
C


ATOM
4811
CG
PRO
B
341
−5.155
−1.955
28.331
1.00
19.20
C


ATOM
4814
CD
PRO
B
341
−3.919
−1.347
28.825
1.00
20.06
C


ATOM
4817
C
PRO
B
341
−3.101
−2.444
25.491
1.00
15.31
C


ATOM
4818
O
PRO
B
341
−3.022
−3.289
24.614
1.00
19.02
O


ATOM
4819
N
GLU
B
342
−2.986
−1.143
25.254
1.00
17.33
N


ATOM
4821
CA
GLU
B
342
−2.745
−0.687
23.906
1.00
17.70
C


ATOM
4823
CB
GLU
B
342
−3.615
0.545
23.555
1.00
20.27
C


ATOM
4826
CG
GLU
B
342
−5.028
0.042
23.191
1.00
21.84
C


ATOM
4829
CD
GLU
B
342
−6.029
1.135
22.803
1.00
30.03
C


ATOM
4830
OE1
GLU
B
342
−5.646
2.108
22.127
1.00
34.62
O


ATOM
4831
OE2
GLU
B
342
−7.217
0.982
23.131
1.00
29.42
O


ATOM
4832
C
GLU
B
342
−1.232
−0.491
23.581
1.00
17.99
C


ATOM
4833
O
GLU
B
342
−0.912
−0.071
22.473
1.00
17.45
O


ATOM
4834
N
ALA
B
343
−0.348
−0.902
24.474
1.00
18.01
N


ATOM
4836
CA
ALA
B
343
1.075
−0.825
24.187
1.00
17.75
C


ATOM
4838
CB
ALA
B
343
1.886
−1.320
25.374
1.00
17.76
C


ATOM
4842
C
ALA
B
343
1.501
−1.509
22.934
1.00
18.41
C


ATOM
4843
O
ALA
B
343
1.018
−2.612
22.539
1.00
16.39
O


ATOM
4844
N
GLN
B
344
2.398
−0.808
22.223
1.00
16.93
N


ATOM
4846
CA
GLN
B
344
3.007
−1.326
21.061
1.00
16.94
C


ATOM
4848
CB
GLN
B
344
3.197
−0.264
19.951
1.00
18.67
C


ATOM
4851
CG
GLN
B
344
1.915
0.367
19.450
1.00
22.45
C


ATOM
4854
CD
GLN
B
344
1.041
−0.668
18.836
1.00
23.74
C


ATOM
4855
OE1
GLN
B
344
1.336
−1.176
17.737
1.00
28.25
O


ATOM
4856
NE2
GLN
B
344
0.022
−1.076
19.570
1.00
24.17
N


ATOM
4859
C
GLN
B
344
4.371
−1.804
21.578
1.00
16.61
C


ATOM
4860
O
GLN
B
344
5.276
−0.987
21.833
1.00
15.55
O


ATOM
4861
N
ILE
B
345
4.503
−3.109
21.745
1.00
14.81
N


ATOM
4863
CA
ILE
B
345
5.747
−3.697
22.338
1.00
15.57
C


ATOM
4865
CB
ILE
B
345
5.426
−4.864
23.229
1.00
14.52
C


ATOM
4867
CG1
ILE
B
345
4.420
−4.437
24.307
1.00
16.03
C


ATOM
4870
CD1
ILE
B
345
4.237
−5.385
25.452
1.00
21.70
C


ATOM
4874
CG2
ILE
B
345
6.683
−5.445
23.832
1.00
18.60
C


ATOM
4878
C
ILE
B
345
6.713
−4.108
21.261
1.00
15.60
C


ATOM
4879
O
ILE
B
345
6.317
−4.759
20.335
1.00
14.53
O


ATOM
4880
N
ILE
B
346
7.947
−3.618
21.338
1.00
15.09
N


ATOM
4882
CA
ILE
B
346
9.006
−3.853
20.373
1.00
17.65
C


ATOM
4884
CB
ILE
B
346
9.607
−2.448
19.887
1.00
18.73
C


ATOM
4886
CG1
ILE
B
346
8.486
−1.515
19.488
1.00
25.44
C


ATOM
4889
CD1
ILE
B
346
7.656
−2.110
18.400
1.00
27.09
C


ATOM
4893
CG2
ILE
B
346
10.538
−2.639
18.736
1.00
24.66
C


ATOM
4897
C
ILE
B
346
10.126
−4.549
21.136
1.00
15.44
C


ATOM
4898
O
ILE
B
346
10.515
−4.048
22.174
1.00
15.03
O


ATOM
4899
N
VAL
B
347
10.621
−5.685
20.637
1.00
15.30
N


ATOM
4901
CA
VAL
B
347
11.701
−6.425
21.310
1.00
14.26
C


ATOM
4903
CB
VAL
B
347
11.363
−7.916
21.411
1.00
13.95
C


ATOM
4905
CG1
VAL
B
347
12.550
−8.744
21.949
1.00
15.52
C


ATOM
4909
CG2
VAL
B
347
10.056
−8.103
22.171
1.00
16.01
C


ATOM
4913
C
VAL
B
347
12.980
−6.253
20.518
1.00
13.34
C


ATOM
4914
O
VAL
B
347
12.999
−6.387
19.275
1.00
13.71
O


ATOM
4915
N
LEU
B
348
14.025
−5.824
21.225
1.00
13.58
N


ATOM
4917
CA
LEU
B
348
15.334
−5.595
20.654
1.00
14.50
C


ATOM
4919
CB
LEU
B
348
15.629
−4.101
20.619
1.00
14.73
C


ATOM
4922
CG
LEU
B
348
14.624
−3.255
19.836
1.00
18.66
C


ATOM
4924
CD1
LEU
B
348
14.885
−1.758
20.172
1.00
20.34
C


ATOM
4928
CD2
LEU
B
348
14.781
−3.515
18.370
1.00
21.48
C


ATOM
4932
C
LEU
B
348
16.420
−6.246
21.500
1.00
13.69
C


ATOM
4933
O
LEU
B
348
16.298
−6.400
22.715
1.00
12.97
O


ATOM
4934
N
PRO
B
349
17.533
−6.626
20.864
1.00
13.80
N


ATOM
4935
CA
PRO
B
349
18.630
−7.173
21.635
1.00
12.71
C


ATOM
4937
CB
PRO
B
349
19.686
−7.528
20.564
1.00
14.22
C


ATOM
4940
CG
PRO
B
349
18.940
−7.614
19.288
1.00
17.12
C


ATOM
4943
CD
PRO
B
349
17.802
−6.586
19.422
1.00
13.86
C


ATOM
4946
C
PRO
B
349
19.238
−6.154
22.603
1.00
12.67
C


ATOM
4947
O
PRO
B
349
19.358
−4.987
22.270
1.00
10.43
O


ATOM
4948
N
VAL
B
350
19.679
−6.629
23.756
1.00
10.88
N


ATOM
4950
CA
VAL
B
350
20.463
−5.844
24.676
1.00
11.84
C


ATOM
4952
CB
VAL
B
350
20.967
−6.740
25.861
1.00
12.85
C


ATOM
4954
CG1
VAL
B
350
21.918
−7.822
25.385
1.00
14.03
C


ATOM
4958
CG2
VAL
B
350
21.614
−5.822
26.921
1.00
13.28
C


ATOM
4962
C
VAL
B
350
21.627
−5.189
23.892
1.00
10.88
C


ATOM
4963
O
VAL
B
350
22.262
−5.815
23.000
1.00
10.87
O


ATOM
4964
N
GLY
B
351
21.864
−3.926
24.205
1.00
11.86
N


ATOM
4966
CA
GLY
B
351
22.882
−3.099
23.581
1.00
11.51
C


ATOM
4969
C
GLY
B
351
22.512
−2.331
22.313
1.00
12.47
C


ATOM
4970
O
GLY
B
351
23.335
−1.618
21.744
1.00
10.98
O


ATOM
4971
N
THR
B
352
21.315
−2.561
21.822
1.00
11.64
N


ATOM
4973
CA
THR
B
352
20.841
−1.861
20.642
1.00
11.92
C


ATOM
4975
CB
THR
B
352
19.508
−2.419
20.225
1.00
13.71
C


ATOM
4977
OG1
THR
B
352
19.641
−3.812
19.860
1.00
10.89
O


ATOM
4979
CG2
THR
B
352
18.993
−1.703
18.981
1.00
12.02
C


ATOM
4983
C
THR
B
352
20.720
−0.383
20.920
1.00
11.24
C


ATOM
4984
O
THR
B
352
20.235
0.015
21.976
1.00
12.17
O


ATOM
4985
N
ILE
B
353
21.218
0.435
20.012
1.00
11.34
N


ATOM
4987
CA
ILE
B
353
21.125
1.893
20.166
1.00
11.58
C


ATOM
4989
CB
ILE
B
353
22.322
2.605
19.504
1.00
13.21
C


ATOM
4991
CG1
ILE
B
353
23.642
2.014
19.992
1.00
11.55
C


ATOM
4994
CD1
ILE
B
353
23.795
1.980
21.496
1.00
15.61
C


ATOM
4998
CG2
ILE
B
353
22.294
4.098
19.802
1.00
13.36
C


ATOM
5002
C
ILE
B
353
19.828
2.332
19.509
1.00
11.44
C


ATOM
5003
O
ILE
B
353
19.485
1.827
18.417
1.00
11.02
O


ATOM
5004
N
VAL
B
354
19.136
3.268
20.180
1.00
10.73
N


ATOM
5006
CA
VAL
B
354
17.785
3.663
19.740
1.00
11.42
C


ATOM
5008
CB
VAL
B
354
16.693
3.043
20.663
1.00
11.30
C


ATOM
5010
CG1
VAL
B
354
16.741
1.521
20.568
1.00
11.88
C


ATOM
5014
CG2
VAL
B
354
16.873
3.542
22.103
1.00
12.49
C


ATOM
5018
C
VAL
B
354
17.558
5.152
19.702
1.00
10.76
C


ATOM
5019
O
VAL
B
354
18.289
5.918
20.294
1.00
11.15
O


ATOM
5020
N
THR
B
355
16.607
5.599
18.894
1.00
10.94
N


ATOM
5022
CA
THR
B
355
16.207
7.001
18.884
1.00
12.16
C


ATOM
5024
CB
THR
B
355
15.004
7.267
17.939
1.00
13.72
C


ATOM
5026
OG1
THR
B
355
13.885
6.481
18.413
1.00
16.24
O


ATOM
5028
CG2
THR
B
355
15.313
6.840
16.561
1.00
14.33
C


ATOM
5032
C
THR
B
355
15.684
7.378
20.254
1.00
12.37
C


ATOM
5033
O
THR
B
355
15.263
6.554
21.050
1.00
11.43
O


ATOM
5034
N
MET
B
356
15.787
8.659
20.540
1.00
12.52
N


ATOM
5036
CA
MET
B
356
15.258
9.222
21.775
1.00
11.53
C


ATOM
5038
CB
MET
B
356
16.311
10.075
22.468
1.00
11.42
C


ATOM
5041
CG
MET
B
356
17.427
9.202
23.035
1.00
12.95
C


ATOM
5044
SD
MET
B
356
16.825
8.040
24.219
1.00
15.08
S


ATOM
5045
CE
MET
B
356
17.963
6.799
24.107
1.00
20.89
C


ATOM
5049
C
MET
B
356
13.952
9.961
21.568
1.00
13.71
C


ATOM
5050
O
MET
B
356
13.675
11.024
22.215
1.00
12.74
O


ATOM
5051
N
GLU
B
357
13.148
9.417
20.659
1.00
14.50
N


ATOM
5053
CA
GLU
B
357
11.765
9.869
20.543
1.00
14.30
C


ATOM
5055
CB
GLU
B
357
11.135
9.357
19.210
1.00
15.52
C


ATOM
5058
CG
GLU
B
357
9.674
9.735
19.153
1.00
15.25
C


ATOM
5061
CD
GLU
B
357
8.947
9.187
17.960
1.00
18.46
C


ATOM
5062
OE1
GLU
B
357
7.768
9.559
17.802
1.00
19.97
O


ATOM
5063
OE2
GLU
B
357
9.543
8.377
17.237
1.00
20.59
O


ATOM
5064
C
GLU
B
357
11.001
9.282
21.703
1.00
14.50
C


ATOM
5065
O
GLU
B
357
11.233
8.123
22.030
1.00
14.99
O


ATOM
5066
N
TYR
B
358
10.129
10.074
22.335
1.00
13.93
N


ATOM
5068
CA
TYR
B
358
9.207
9.612
23.363
1.00
14.38
C


ATOM
5070
CB
TYR
B
358
9.026
10.601
24.499
1.00
14.43
C


ATOM
5073
CG
TYR
B
358
8.057
10.136
25.563
1.00
12.84
C


ATOM
5074
CD1
TYR
B
358
8.436
9.165
26.487
1.00
14.52
C


ATOM
5076
CE1
TYR
B
358
7.559
8.706
27.463
1.00
19.65
C


ATOM
5078
CZ
TYR
B
358
6.266
9.240
27.504
1.00
19.95
C


ATOM
5079
OH
TYR
B
358
5.321
8.903
28.447
1.00
26.07
O


ATOM
5081
CE2
TYR
B
358
5.873
10.184
26.607
1.00
16.96
C


ATOM
5083
CD2
TYR
B
358
6.767
10.650
25.643
1.00
18.09
C


ATOM
5085
C
TYR
B
358
7.867
9.326
22.712
1.00
15.23
C


ATOM
5086
O
TYR
B
358
7.142
10.261
22.340
1.00
14.02
O


ATOM
5087
N
ARG
B
359
7.551
8.048
22.606
1.00
15.15
N


ATOM
5089
CA
ARG
B
359
6.294
7.557
22.003
1.00
16.88
C


ATOM
5091
CB
ARG
B
359
6.615
6.465
20.998
1.00
18.79
C


ATOM
5094
CG
ARG
B
359
6.824
6.884
19.703
1.00
22.92
C


ATOM
5097
CD
ARG
B
359
6.435
5.794
18.692
1.00
25.80
C


ATOM
5100
NE
ARG
B
359
7.237
6.083
17.556
1.00
25.82
N


ATOM
5102
CZ
ARG
B
359
7.515
5.241
16.593
1.00
26.84
C


ATOM
5103
NH1
ARG
B
359
7.000
3.996
16.593
1.00
26.53
N


ATOM
5106
NH2
ARG
B
359
8.297
5.668
15.627
1.00
28.90
N


ATOM
5109
C
ARG
B
359
5.449
6.946
23.095
1.00
17.14
C


ATOM
5110
O
ARG
B
359
5.767
5.861
23.625
1.00
15.98
O


ATOM
5111
N
ILE
B
360
4.401
7.656
23.504
1.00
16.16
N


ATOM
5113
CA
ILE
B
360
3.633
7.262
24.670
1.00
17.53
C


ATOM
5115
CB
ILE
B
360
2.575
8.354
24.998
1.00
17.66
C


ATOM
5117
CG1
ILE
B
360
1.895
8.067
26.333
1.00
24.45
C


ATOM
5120
CD1
ILE
B
360
1.711
9.278
27.140
1.00
28.72
C


ATOM
5124
CG2
ILE
B
360
1.506
8.417
23.930
1.00
18.76
C


ATOM
5128
C
ILE
B
360
2.947
5.899
24.595
1.00
16.17
C


ATOM
5129
O
ILE
B
360
2.649
5.307
25.629
1.00
19.59
O


ATOM
5130
N
ASP
B
361
2.692
5.431
23.402
1.00
19.19
N


ATOM
5132
CA
ASP
B
361
2.049
4.131
23.290
1.00
20.04
C


ATOM
5134
CB
ASP
B
361
1.070
4.141
22.156
1.00
22.33
C


ATOM
5137
CG
ASP
B
361
−0.210
4.932
22.509
1.00
27.91
C


ATOM
5138
OD1
ASP
B
361
−0.701
4.823
23.693
1.00
33.70
O


ATOM
5139
OD2
ASP
B
361
−0.734
5.711
21.687
1.00
38.62
O


ATOM
5140
C
ASP
B
361
3.025
2.996
23.111
1.00
19.38
C


ATOM
5141
O
ASP
B
361
2.580
1.872
22.958
1.00
18.79
O


ATOM
5142
N
ARG
B
362
4.342
3.291
23.099
1.00
15.34
N


ATOM
5144
CA
ARG
B
362
5.342
2.241
22.870
1.00
15.87
C


ATOM
5146
CB
ARG
B
362
6.480
2.793
21.992
1.00
13.90
C


ATOM
5149
CG
ARG
B
362
7.657
1.839
21.867
1.00
16.63
C


ATOM
5152
CD
ARG
B
362
8.570
2.234
20.721
1.00
16.00
C


ATOM
5155
NE
ARG
B
362
9.204
3.494
21.085
1.00
15.40
N


ATOM
5157
CZ
ARG
B
362
9.808
4.315
20.234
1.00
14.22
C


ATOM
5158
NH1
ARG
B
362
9.893
4.014
18.939
1.00
16.86
N


ATOM
5161
NH2
ARG
B
362
10.295
5.449
20.686
1.00
14.53
N


ATOM
5164
C
ARG
B
362
5.970
1.737
24.163
1.00
14.75
C


ATOM
5165
O
ARG
B
362
6.130
2.504
25.117
1.00
13.39
O


ATOM
5166
N
VAL
B
363
6.285
0.467
24.199
1.00
13.88
N


ATOM
5168
CA
VAL
B
363
7.159
−0.094
25.241
1.00
14.50
C


ATOM
5170
CB
VAL
B
363
6.448
−0.910
26.349
1.00
14.42
C


ATOM
5172
CG1
VAL
B
363
7.477
−1.351
27.372
1.00
17.68
C


ATOM
5176
CG2
VAL
B
363
5.401
−0.069
27.015
1.00
16.62
C


ATOM
5180
C
VAL
B
363
8.230
−0.943
24.576
1.00
14.33
C


ATOM
5181
O
VAL
B
363
7.928
−1.989
23.984
1.00
14.74
O


ATOM
5182
N
ARG
B
364
9.483
−0.510
24.651
1.00
13.57
N


ATOM
5184
CA
ARG
B
364
10.570
−1.349
24.131
1.00
13.59
C


ATOM
5186
CB
ARG
B
364
11.753
−0.458
23.713
1.00
12.88
C


ATOM
5189
CG
ARG
B
364
11.565
0.380
22.482
1.00
14.21
C


ATOM
5192
CD
ARG
B
364
12.671
1.411
22.216
1.00
18.75
C


ATOM
5195
NE
ARG
B
364
12.606
2.066
20.950
1.00
16.44
N


ATOM
5197
CZ
ARG
B
364
13.123
3.278
20.638
1.00
18.34
C


ATOM
5198
NH1
ARG
B
364
13.591
4.073
21.531
1.00
21.95
N


ATOM
5201
NH2
ARG
B
364
13.073
3.670
19.412
1.00
23.97
N


ATOM
5204
C
ARG
B
364
11.046
−2.339
25.177
1.00
14.09
C


ATOM
5205
O
ARG
B
364
11.154
−1.994
26.361
1.00
16.64
O


ATOM
5206
N
LEU
B
365
11.340
−3.548
24.735
1.00
13.84
N


ATOM
5208
CA
LEU
B
365
11.904
−4.519
25.617
1.00
12.64
C


ATOM
5210
CB
LEU
B
365
11.017
−5.737
25.667
1.00
14.17
C


ATOM
5213
CG
LEU
B
365
9.653
−5.571
26.305
1.00
14.30
C


ATOM
5215
CD1
LEU
B
365
8.907
−6.860
26.267
1.00
17.46
C


ATOM
5219
CD2
LEU
B
365
9.813
−5.054
27.714
1.00
18.97
C


ATOM
5223
C
LEU
B
365
13.235
−4.929
25.065
1.00
13.48
C


ATOM
5224
O
LEU
B
365
13.307
−5.386
23.919
1.00
13.99
O


ATOM
5225
N
PHE
B
366
14.271
−4.775
25.887
1.00
12.70
N


ATOM
5227
CA
PHE
B
366
15.619
−5.176
25.533
1.00
12.04
C


ATOM
5229
CB
PHE
B
366
16.651
−4.148
25.971
1.00
12.03
C


ATOM
5232
CG
PHE
B
366
16.476
−2.809
25.291
1.00
13.71
C


ATOM
5233
CD1
PHE
B
366
15.591
−1.889
25.820
1.00
13.42
C


ATOM
5235
CE1
PHE
B
366
15.373
−0.702
25.229
1.00
16.78
C


ATOM
5237
CZ
PHE
B
366
16.025
−0.376
24.078
1.00
16.52
C


ATOM
5239
CE2
PHE
B
366
16.899
−1.322
23.444
1.00
15.87
C


ATOM
5241
CD2
PHE
B
366
17.121
−2.554
24.083
1.00
15.60
C


ATOM
5243
C
PHE
B
366
15.875
−6.502
26.202
1.00
13.03
C


ATOM
5244
O
PHE
B
366
15.700
−6.682
27.407
1.00
13.72
O


ATOM
5245
N
VAL
B
367
16.319
−7.448
25.407
1.00
13.72
N


ATOM
5247
CA
VAL
B
367
16.457
−8.824
25.906
1.00
14.35
C


ATOM
5249
CB
VAL
B
367
15.408
−9.719
25.263
1.00
15.42
C


ATOM
5251
CG1
VAL
B
367
13.988
−9.325
25.626
1.00
17.97
C


ATOM
5255
CG2
VAL
B
367
15.608
−9.853
23.736
1.00
13.87
C


ATOM
5259
C
VAL
B
367
17.829
−9.418
25.654
1.00
14.47
C


ATOM
5260
O
VAL
B
367
18.508
−9.099
24.686
1.00
13.82
O


ATOM
5261
N
ASP
B
368
18.186
−10.385
26.500
1.00
15.21
N


ATOM
5263
CA
ASP
B
368
19.421
−11.145
26.337
1.00
14.26
C


ATOM
5265
CB
ASP
B
368
20.020
−11.526
27.668
1.00
12.83
C


ATOM
5268
CG
ASP
B
368
19.168
−12.492
28.461
1.00
13.78
C


ATOM
5269
OD1
ASP
B
368
18.383
−13.277
27.864
1.00
15.03
O


ATOM
5270
OD2
ASP
B
368
19.284
−12.487
29.687
1.00
14.11
O


ATOM
5271
C
ASP
B
368
19.197
−12.347
25.438
1.00
15.37
C


ATOM
5272
O
ASP
B
368
18.090
−12.572
24.940
1.00
13.77
O


ATOM
5273
N
LYS
B
369
20.201
−13.219
25.295
1.00
15.53
N


ATOM
5275
CA
LYS
B
369
20.096
−14.274
24.320
1.00
16.17
C


ATOM
5277
CB
LYS
B
369
21.492
−14.913
24.031
1.00
18.57
C


ATOM
5280
CG
LYS
B
369
22.335
−14.040
23.132
1.00
23.43
C


ATOM
5283
CD
LYS
B
369
21.692
−13.930
21.723
1.00
33.33
C


ATOM
5286
CE
LYS
B
369
21.825
−15.245
20.863
1.00
37.24
C


ATOM
5289
NZ
LYS
B
369
20.834
−15.304
19.703
1.00
39.15
N


ATOM
5293
C
LYS
B
369
19.120
−15.342
24.746
1.00
17.09
C


ATOM
5294
O
LYS
B
369
18.736
−16.166
23.914
1.00
16.83
O


ATOM
5295
N
LEU
B
370
18.744
−15.352
26.029
1.00
15.51
N


ATOM
5297
CA
LEU
B
370
17.739
−16.298
26.509
1.00
15.40
C


ATOM
5299
CB
LEU
B
370
18.053
−16.814
27.899
1.00
15.53
C


ATOM
5302
CG
LEU
B
370
19.358
−17.657
28.054
1.00
17.89
C


ATOM
5304
CD1
LEU
B
370
19.624
−17.980
29.490
1.00
16.28
C


ATOM
5308
CD2
LEU
B
370
19.237
−18.944
27.314
1.00
17.77
C


ATOM
5312
C
LEU
B
370
16.346
−15.711
26.481
1.00
17.02
C


ATOM
5313
O
LEU
B
370
15.426
−16.340
27.005
1.00
16.78
O


ATOM
5314
N
ASP
B
371
16.191
−14.538
25.884
1.00
15.62
N


ATOM
5316
CA
ASP
B
371
14.909
−13.828
25.822
1.00
17.82
C


ATOM
5318
CB
ASP
B
371
13.844
−14.610
25.070
1.00
16.70
C


ATOM
5321
CG
ASP
B
371
13.943
−14.455
23.561
1.00
26.05
C


ATOM
5322
OD1
ASP
B
371
14.579
−13.492
23.026
1.00
26.78
O


ATOM
5323
OD2
ASP
B
371
13.333
−15.252
22.835
1.00
35.44
O


ATOM
5324
C
ASP
B
371
14.392
−13.390
27.197
1.00
16.31
C


ATOM
5325
O
ASP
B
371
13.179
−13.251
27.432
1.00
15.98
O


ATOM
5326
N
ASN
B
372
15.334
−13.152
28.115
1.00
14.12
N


ATOM
5328
CA
ASN
B
372
15.057
−12.509
29.380
1.00
13.45
C


ATOM
5330
CB
ASN
B
372
15.846
−13.137
30.518
1.00
14.25
C


ATOM
5333
CG
ASN
B
372
15.359
−14.530
30.868
1.00
17.78
C


ATOM
5334
OD1
ASN
B
372
14.170
−14.830
30.726
1.00
15.50
O


ATOM
5335
ND2
ASN
B
372
16.280
−15.394
31.268
1.00
15.27
N


ATOM
5338
C
ASN
B
372
15.328
−11.025
29.328
1.00
13.56
C


ATOM
5339
O
ASN
B
372
16.185
−10.535
28.526
1.00
12.51
O


ATOM
5340
N
ILE
B
373
14.521
−10.271
30.078
1.00
12.00
N


ATOM
5342
CA
ILE
B
373
14.595
−8.793
30.098
1.00
12.80
C


ATOM
5344
CB
ILE
B
373
13.409
−8.177
30.868
1.00
12.48
C


ATOM
5346
CG1
ILE
B
373
12.115
−8.624
30.263
1.00
14.38
C


ATOM
5349
CD1
ILE
B
373
12.021
−8.386
28.913
1.00
13.59
C


ATOM
5353
CG2
ILE
B
373
13.467
−6.685
30.862
1.00
13.18
C


ATOM
5357
C
ILE
B
373
15.888
−8.319
30.716
1.00
12.70
C


ATOM
5358
O
ILE
B
373
16.217
−8.667
31.872
1.00
13.25
O


ATOM
5359
N
ALA
B
374
16.619
−7.490
29.940
1.00
12.49
N


ATOM
5361
CA
ALA
B
374
17.962
−7.065
30.334
1.00
11.98
C


ATOM
5363
CB
ALA
B
374
18.894
−6.974
29.099
1.00
11.19
C


ATOM
5367
C
ALA
B
374
18.005
−5.723
31.031
1.00
12.88
C


ATOM
5368
O
ALA
B
374
19.017
−5.394
31.640
1.00
13.61
O


ATOM
5369
N
GLU
B
375
16.957
−4.922
30.954
1.00
12.63
N


ATOM
5371
CA
GLU
B
375
16.927
−3.628
31.592
1.00
11.71
C


ATOM
5373
CB
GLU
B
375
17.669
−2.571
30.740
1.00
13.04
C


ATOM
5376
CG
GLU
B
375
17.020
−2.247
29.443
1.00
13.36
C


ATOM
5379
CD
GLU
B
375
17.854
−1.238
28.611
1.00
16.47
C


ATOM
5380
OE1
GLU
B
375
18.961
−1.591
28.279
1.00
19.52
O


ATOM
5381
OE2
GLU
B
375
17.355
−0.152
28.242
1.00
18.89
O


ATOM
5382
C
GLU
B
375
15.464
−3.195
31.858
1.00
12.84
C


ATOM
5383
O
GLU
B
375
14.563
−3.750
31.276
1.00
12.27
O


ATOM
5384
N
VAL
B
376
15.277
−2.235
32.747
1.00
12.90
N


ATOM
5386
CA
VAL
B
376
13.918
−1.872
33.175
1.00
13.25
C


ATOM
5388
CB
VAL
B
376
13.941
−0.719
34.173
1.00
13.63
C


ATOM
5390
CG1
VAL
B
376
12.515
−0.240
34.514
1.00
15.72
C


ATOM
5394
CG2
VAL
B
376
14.681
−1.129
35.424
1.00
19.49
C


ATOM
5398
C
VAL
B
376
13.110
−1.441
32.018
1.00
12.36
C


ATOM
5399
O
VAL
B
376
13.458
−0.494
31.358
1.00
12.35
O


ATOM
5400
N
PRO
B
377
12.016
−2.117
31.700
1.00
13.27
N


ATOM
5401
CA
PRO
B
377
11.169
−1.588
30.649
1.00
14.34
C


ATOM
5403
CB
PRO
B
377
10.141
−2.697
30.437
1.00
15.95
C


ATOM
5406
CG
PRO
B
377
10.777
−3.950
31.039
1.00
13.76
C


ATOM
5409
CD
PRO
B
377
11.585
−3.453
32.170
1.00
13.52
C


ATOM
5412
C
PRO
B
377
10.472
−0.302
31.046
1.00
14.46
C


ATOM
5413
O
PRO
B
377
10.078
−0.158
32.190
1.00
14.50
O


ATOM
5414
N
ARG
B
378
10.291
0.603
30.087
1.00
14.99
N


ATOM
5416
CA
ARG
B
378
9.687
1.912
30.298
1.00
14.79
C


ATOM
5418
CB
ARG
B
378
10.756
3.004
30.386
1.00
14.41
C


ATOM
5421
CG
ARG
B
378
11.717
2.809
31.542
1.00
16.49
C


ATOM
5424
CD
ARG
B
378
12.848
3.892
31.600
1.00
17.97
C


ATOM
5427
NE
ARG
B
378
13.805
3.569
32.642
1.00
15.14
N


ATOM
5429
CZ
ARG
B
378
13.602
3.673
33.949
1.00
19.35
C


ATOM
5430
NH1
ARG
B
378
12.452
4.173
34.445
1.00
21.30
N


ATOM
5433
NH2
ARG
B
378
14.548
3.250
34.752
1.00
20.05
N


ATOM
5436
C
ARG
B
378
8.797
2.263
29.129
1.00
14.34
C


ATOM
5437
O
ARG
B
378
8.974
1.810
28.013
1.00
14.31
O


ATOM
5438
N
VAL
B
379
7.801
3.052
29.411
1.00
13.60
N


ATOM
5440
CA
VAL
B
379
7.028
3.650
28.358
1.00
14.99
C


ATOM
5442
CB
VAL
B
379
5.847
4.399
28.967
1.00
16.05
C


ATOM
5444
CG1
VAL
B
379
5.148
5.163
27.928
1.00
17.97
C


ATOM
5448
CG2
VAL
B
379
4.863
3.410
29.606
1.00
19.07
C


ATOM
5452
C
VAL
B
379
7.899
4.629
27.551
1.00
13.40
C


ATOM
5453
O
VAL
B
379
8.683
5.397
28.127
1.00
13.64
O


ATOM
5454
N
GLY
B
380
7.742
4.666
26.241
1.00
13.02
N


ATOM
5456
CA
GLY
B
380
8.480
5.637
25.459
1.00
14.94
C


ATOM
5459
C
GLY
B
380
8.968
5.181
24.107
1.00
13.89
C


ATOM
5460
O
GLY
B
380
8.939
3.974
23.839
1.00
13.82
O


ATOM
5461
OXT
GLY
B
380
9.391
6.068
23.366
1.00
15.44
O


ATOM
5462
O
HOH
W
1
26.337
16.956
29.710
1.00
8.65
O


ATOM
5465
O
HOH
W
2
9.939
2.105
25.538
1.00
12.99
O


ATOM
5468
O
HOH
W
3
22.328
10.101
24.400
1.00
12.55
O


ATOM
5471
O
HOH
W
4
30.572
18.292
23.118
1.00
9.33
O


ATOM
5474
O
HOH
W
5
8.147
23.150
18.782
1.00
14.00
O


ATOM
5477
O
HOH
W
6
11.956
29.794
31.575
1.00
15.66
O


ATOM
5480
O
HOH
W
7
36.742
23.674
17.265
1.00
14.06
O


ATOM
5483
O
HOH
W
8
26.462
19.745
37.226
1.00
12.03
O


ATOM
5486
O
HOH
W
9
23.101
0.721
12.656
1.00
12.19
O


ATOM
5489
O
HOH
W
10
20.065
−2.650
26.156
1.00
20.32
O


ATOM
5492
O
HOH
W
11
18.435
36.223
15.049
1.00
20.55
O


ATOM
5495
O
HOH
W
12
18.961
−14.287
31.415
1.00
12.28
O


ATOM
5498
O
HOH
W
13
13.655
−3.630
28.503
1.00
11.25
O


ATOM
5501
O
HOH
W
14
6.772
28.494
24.564
1.00
16.02
O


ATOM
5504
O
HOH
W
15
25.827
−0.949
23.096
1.00
13.13
O


ATOM
5507
O
HOH
W
16
10.548
23.630
17.206
1.00
15.05
O


ATOM
5510
O
HOH
W
17
21.366
−0.008
27.896
1.00
14.00
O


ATOM
5513
O
HOH
W
18
6.571
29.390
22.100
1.00
18.38
O


ATOM
5516
O
HOH
W
19
25.418
4.779
24.010
1.00
11.22
O


ATOM
5519
O
HOH
W
20
15.446
15.813
32.417
1.00
13.39
O


ATOM
5522
O
HOH
W
21
5.625
22.360
17.718
1.00
14.91
O


ATOM
5525
O
HOH
W
22
27.953
5.617
25.060
1.00
12.69
O


ATOM
5528
O
HOH
W
23
13.200
17.441
16.128
1.00
12.98
O


ATOM
5531
O
HOH
W
24
42.359
19.143
18.719
1.00
14.71
O


ATOM
5534
O
HOH
W
25
24.537
−2.025
19.216
1.00
12.97
O


ATOM
5537
O
HOH
W
26
27.926
25.732
6.249
1.00
17.25
O


ATOM
5540
O
HOH
W
27
39.025
23.474
22.653
1.00
12.82
O


ATOM
5543
O
HOH
W
28
23.815
15.465
37.744
1.00
13.10
O


ATOM
5546
O
HOH
W
29
18.367
−9.817
33.092
1.00
17.61
O


ATOM
5549
O
HOH
W
30
20.380
12.554
15.652
1.00
10.66
O


ATOM
5552
O
HOH
W
31
18.651
1.596
26.271
1.00
14.85
O


ATOM
5555
O
HOH
W
32
35.209
6.007
10.838
1.00
15.93
O


ATOM
5558
O
HOH
W
33
18.465
24.874
35.632
1.00
11.70
O


ATOM
5561
O
HOH
W
34
20.815
27.470
36.539
1.00
14.90
O


ATOM
5564
O
HOH
W
35
20.733
10.911
9.565
1.00
14.64
O


ATOM
5567
O
HOH
W
36
4.788
27.744
28.587
1.00
16.40
O


ATOM
5570
O
HOH
W
37
8.972
17.007
35.207
1.00
15.88
O


ATOM
5573
O
HOH
W
38
33.433
11.882
27.279
1.00
15.71
O


ATOM
5576
O
HOH
W
39
11.974
0.247
27.662
1.00
14.69
O


ATOM
5579
O
HOH
W
40
11.026
22.081
14.883
1.00
14.23
O


ATOM
5582
O
HOH
W
41
26.884
−0.716
26.776
1.00
14.35
O


ATOM
5585
O
HOH
W
42
41.266
13.179
18.993
1.00
17.71
O


ATOM
5588
O
HOH
W
43
27.981
−2.861
17.044
1.00
20.05
O


ATOM
5591
O
HOH
W
44
2.212
−4.670
21.196
1.00
17.41
O


ATOM
5594
O
HOH
W
45
5.416
16.195
31.844
1.00
15.02
O


ATOM
5597
O
HOH
W
46
20.229
32.354
26.988
1.00
15.42
O


ATOM
5600
O
HOH
W
47
27.214
14.437
31.277
1.00
10.24
O


ATOM
5603
O
HOH
W
48
24.332
32.917
12.832
1.00
16.80
O


ATOM
5606
O
HOH
W
49
9.986
5.426
33.075
1.00
16.06
O


ATOM
5609
O
HOH
W
50
21.134
30.372
36.728
1.00
15.50
O


ATOM
5612
O
HOH
W
51
4.815
20.991
24.941
1.00
17.09
O


ATOM
5615
O
HOH
W
52
39.195
8.284
21.866
1.00
17.72
O


ATOM
5618
O
HOH
W
53
24.661
0.260
25.378
1.00
13.02
O


ATOM
5621
O
HOH
W
54
6.599
11.219
19.732
1.00
15.01
O


ATOM
5624
O
HOH
W
55
−1.402
−3.742
22.540
1.00
17.94
O


ATOM
5627
O
HOH
W
56
23.967
19.287
36.011
1.00
12.16
O


ATOM
5630
O
HOH
W
57
32.403
10.495
30.395
1.00
18.72
O


ATOM
5633
O
HOH
W
58
30.411
20.433
3.925
1.00
19.29
O


ATOM
5636
O
HOH
W
59
14.110
5.986
23.754
1.00
21.44
O


ATOM
5639
O
HOH
W
60
9.502
31.255
31.238
1.00
19.33
O


ATOM
5642
O
HOH
W
61
17.881
7.531
29.614
1.00
18.16
O


ATOM
5645
O
HOH
W
62
35.920
21.100
27.416
1.00
18.14
O


ATOM
5648
O
HOH
W
63
21.184
17.585
43.689
1.00
19.63
O


ATOM
5651
O
HOH
W
64
11.422
6.357
17.386
1.00
17.47
O


ATOM
5654
O
HOH
W
65
7.568
24.486
1.717
1.00
26.99
O


ATOM
5657
O
HOH
W
66
11.835
23.396
12.551
1.00
17.33
O


ATOM
5660
O
HOH
W
67
15.674
−0.267
13.102
1.00
28.14
O


ATOM
5663
O
HOH
W
68
35.246
4.205
18.695
1.00
18.92
O


ATOM
5666
O
HOH
W
69
12.071
7.219
35.891
1.00
17.95
O


ATOM
5669
O
HOH
W
70
33.151
1.122
14.747
1.00
19.80
O


ATOM
5672
O
HOH
W
71
22.406
17.015
36.075
1.00
12.42
O


ATOM
5675
O
HOH
W
72
20.744
5.444
32.336
1.00
24.35
O


ATOM
5678
O
HOH
W
73
20.988
−0.601
11.337
1.00
17.02
O


ATOM
5681
O
HOH
W
74
32.168
21.857
32.986
1.00
16.30
O


ATOM
5684
O
HOH
W
75
28.340
34.388
18.531
1.00
14.67
O


ATOM
5687
O
HOH
W
76
27.395
34.930
22.269
1.00
15.72
O


ATOM
5690
O
HOH
W
77
−2.881
1.257
27.203
1.00
24.36
O


ATOM
5693
O
HOH
W
78
1.320
22.974
26.514
1.00
16.10
O


ATOM
5696
O
HOH
W
79
20.014
2.049
4.590
1.00
18.49
O


ATOM
5699
O
HOH
W
80
20.131
−10.521
22.803
1.00
16.67
O


ATOM
5702
O
HOH
W
81
2.801
−12.938
26.738
1.00
21.33
O


ATOM
5705
O
HOH
W
82
13.524
14.422
42.122
1.00
22.70
O


ATOM
5708
O
HOH
W
83
41.288
13.787
8.384
1.00
21.34
O


ATOM
5711
O
HOH
W
84
33.383
31.044
25.382
1.00
20.83
O


ATOM
5714
O
HOH
W
85
39.402
29.928
19.487
1.00
19.25
O


ATOM
5717
O
HOH
W
86
14.181
−0.702
28.733
1.00
20.17
O


ATOM
5720
O
HOH
W
87
11.217
5.903
27.361
1.00
18.74
O


ATOM
5723
O
HOH
W
88
28.627
31.304
33.345
1.00
18.56
O


ATOM
5726
O
HOH
W
89
25.546
35.322
19.001
1.00
21.03
O


ATOM
5729
O
HOH
W
90
17.693
−0.865
33.818
1.00
23.45
O


ATOM
5732
O
HOH
W
91
16.853
2.781
26.050
1.00
20.34
O


ATOM
5735
O
HOH
W
92
34.612
21.168
14.066
1.00
15.33
O


ATOM
5738
O
HOH
W
93
19.619
19.119
0.936
1.00
29.35
O


ATOM
5741
O
HOH
W
94
20.721
21.727
42.621
1.00
20.03
O


ATOM
5744
O
HOH
W
95
17.040
19.477
42.629
1.00
20.78
O


ATOM
5747
O
HOH
W
96
20.111
−0.122
24.735
1.00
19.14
O


ATOM
5750
O
HOH
W
97
3.609
25.498
17.596
1.00
22.36
O


ATOM
5753
O
HOH
W
98
22.201
34.610
13.464
1.00
19.68
O


ATOM
5756
O
HOH
W
99
1.306
27.285
34.611
1.00
28.89
O


ATOM
5759
O
HOH
W
100
1.618
26.901
31.789
1.00
16.56
O


ATOM
5762
O
HOH
W
101
34.765
7.143
28.884
1.00
25.03
O


ATOM
5765
O
HOH
W
102
39.459
5.374
23.644
1.00
21.05
O


ATOM
5768
O
HOH
W
103
7.666
6.005
10.390
1.00
23.68
O


ATOM
5771
O
HOH
W
104
25.629
6.850
39.327
1.00
25.93
O


ATOM
5774
O
HOH
W
105
4.516
10.592
22.714
1.00
16.32
O


ATOM
5777
O
HOH
W
106
23.065
−8.147
22.078
1.00
22.91
O


ATOM
5780
O
HOH
W
107
15.215
5.229
8.027
1.00
18.64
O


ATOM
5783
O
HOH
W
108
9.120
−7.084
18.432
1.00
24.86
O


ATOM
5786
O
HOH
W
109
9.059
32.376
26.670
1.00
22.20
O


ATOM
5789
O
HOH
W
110
28.414
12.755
0.995
1.00
20.29
O


ATOM
5792
O
HOH
W
111
14.310
19.153
−3.544
1.00
25.42
O


ATOM
5795
O
HOH
W
112
18.007
−11.145
20.744
1.00
25.31
O


ATOM
5798
O
HOH
W
113
38.102
21.625
11.695
1.00
21.89
O


ATOM
5801
O
HOH
W
114
6.120
13.736
30.142
1.00
24.46
O


ATOM
5804
O
HOH
W
115
15.906
−18.386
31.627
1.00
22.15
O


ATOM
5807
O
HOH
W
116
22.389
33.907
19.142
1.00
22.92
O


ATOM
5810
O
HOH
W
117
32.200
35.724
21.334
1.00
26.32
O


ATOM
5813
O
HOH
W
118
2.190
17.510
6.678
1.00
25.22
O


ATOM
5816
O
HOH
W
119
1.118
4.704
29.359
1.00
25.72
O


ATOM
5819
O
HOH
W
120
9.611
1.556
17.312
1.00
23.94
O


ATOM
5822
O
HOH
W
121
3.629
23.370
16.136
1.00
22.93
O


ATOM
5825
O
HOH
W
122
32.907
25.232
8.564
1.00
22.34
O


ATOM
5828
O
HOH
W
123
−1.806
−9.230
29.885
1.00
26.14
O


ATOM
5831
O
HOH
W
124
32.230
21.567
30.291
1.00
23.29
O


ATOM
5834
O
HOH
W
125
37.450
14.678
29.329
1.00
25.24
O


ATOM
5837
O
HOH
W
126
15.339
36.760
18.883
1.00
20.61
O


ATOM
5840
O
HOH
W
127
10.235
7.567
31.063
1.00
21.51
O


ATOM
5843
O
HOH
W
128
24.897
37.442
19.549
1.00
24.86
O


ATOM
5846
O
HOH
W
129
17.030
−13.244
22.390
1.00
21.48
O


ATOM
5849
O
HOH
W
130
9.040
8.412
14.673
1.00
27.72
O


ATOM
5852
O
HOH
W
131
14.720
31.661
34.320
1.00
22.71
O


ATOM
5855
O
HOH
W
132
19.535
8.925
0.482
1.00
21.18
O


ATOM
5858
O
HOH
W
133
12.077
0.962
18.542
1.00
22.23
O


ATOM
5861
O
HOH
W
134
3.441
12.279
24.403
1.00
21.97
O


ATOM
5864
O
HOH
W
135
13.235
21.344
−4.722
1.00
25.46
O


ATOM
5867
O
HOH
W
136
4.989
14.655
27.582
1.00
28.75
O


ATOM
5870
O
HOH
W
137
−0.388
24.768
31.623
1.00
23.62
O


ATOM
5873
O
HOH
W
138
9.733
10.653
39.989
1.00
28.29
O


ATOM
5876
O
HOH
W
139
5.022
2.800
18.295
1.00
34.14
O


ATOM
5879
O
HOH
W
140
26.430
−3.864
19.091
1.00
26.57
O


ATOM
5882
O
HOH
W
141
33.127
12.459
35.197
1.00
24.55
O


ATOM
5885
O
HOH
W
142
4.529
14.653
24.602
1.00
27.79
O


ATOM
5888
O
HOH
W
143
34.889
3.006
29.679
1.00
26.45
O


ATOM
5891
O
HOH
W
144
26.472
27.781
5.265
1.00
24.30
O


ATOM
5894
O
HOH
W
145
9.844
1.973
10.352
1.00
30.48
O


ATOM
5897
O
HOH
W
146
23.113
35.988
15.667
1.00
24.26
O


ATOM
5900
O
HOH
W
147
3.506
18.934
26.329
1.00
23.36
O


ATOM
5903
O
HOH
W
148
41.932
12.669
10.942
1.00
26.60
O


ATOM
5906
O
HOH
W
149
40.619
12.671
22.354
1.00
26.08
O


ATOM
5909
O
HOH
W
150
33.062
38.217
15.479
1.00
37.76
O


ATOM
5912
O
HOH
W
151
3.554
31.071
10.635
1.00
26.96
O


ATOM
5915
O
HOH
W
152
14.084
8.928
41.767
1.00
28.97
O


ATOM
5918
O
HOH
W
153
29.827
1.222
9.591
1.00
30.85
O


ATOM
5921
O
HOH
W
154
23.088
−1.722
26.918
1.00
27.80
O


ATOM
5924
O
HOH
W
155
8.435
8.580
36.298
1.00
27.72
O


ATOM
5927
O
HOH
W
156
42.926
15.621
8.285
1.00
29.85
O


ATOM
5930
O
HOH
W
157
6.654
11.279
36.797
1.00
28.44
O


ATOM
5933
O
HOH
W
158
15.300
6.642
27.237
1.00
23.08
O


ATOM
5936
O
HOH
W
159
14.085
−11.817
20.799
1.00
27.86
O


ATOM
5939
O
HOH
W
160
−1.521
−6.774
36.408
1.00
31.93
O


ATOM
5942
O
HOH
W
161
15.519
1.708
31.201
1.00
27.02
O


ATOM
5945
O
HOH
W
162
0.621
−5.366
35.349
1.00
28.46
O


ATOM
5948
O
HOH
W
163
18.036
−12.751
33.648
1.00
22.09
O


ATOM
5951
O
HOH
W
164
32.843
18.669
30.344
1.00
28.48
O


ATOM
5954
O
HOH
W
165
27.765
11.874
38.295
1.00
18.10
O


ATOM
5957
O
HOH
W
166
1.781
17.606
13.084
1.00
28.85
O


ATOM
5960
O
HOH
W
167
20.211
0.158
6.249
1.00
29.98
O


ATOM
5963
O
HOH
W
168
2.759
19.112
32.488
1.00
24.07
O


ATOM
5966
O
HOH
W
169
33.968
18.793
32.524
1.00
23.54
O


ATOM
5969
O
HOH
W
170
−1.571
−13.592
26.165
1.00
34.45
O


ATOM
5972
O
HOH
W
171
39.370
28.627
17.071
1.00
26.29
O


ATOM
5975
O
HOH
W
172
17.376
32.794
35.436
1.00
23.98
O


ATOM
5978
O
HOH
W
173
9.391
6.761
35.029
1.00
21.74
O


ATOM
5981
O
HOH
W
174
16.352
11.687
43.877
1.00
31.51
O


ATOM
5984
O
HOH
W
175
36.018
4.292
25.853
1.00
21.19
O


ATOM
5987
O
HOH
W
176
24.899
−2.518
10.289
1.00
21.66
O


ATOM
5990
O
HOH
W
177
−1.286
2.934
25.647
1.00
31.88
O


ATOM
5993
O
HOH
W
178
13.449
−16.540
28.665
1.00
26.26
O


ATOM
5996
O
HOH
W
179
13.301
−0.613
16.357
1.00
27.05
O


ATOM
5999
O
HOH
W
180
24.842
17.585
44.930
1.00
26.85
O


ATOM
6002
O
HOH
W
181
5.856
18.874
39.008
1.00
29.19
O


ATOM
6005
O
HOH
W
182
−1.630
2.543
31.697
1.00
33.70
O


ATOM
6008
O
HOH
W
183
38.130
17.164
1.491
1.00
33.90
O


ATOM
6011
O
HOH
W
184
38.533
33.710
21.252
1.00
23.89
O


ATOM
6014
O
HOH
W
185
8.687
18.331
1.042
1.00
28.46
O


ATOM
6017
O
HOH
W
186
13.162
5.211
37.558
1.00
29.88
O


ATOM
6020
O
HOH
W
187
13.148
−16.170
33.001
1.00
24.01
O


ATOM
6023
O
HOH
W
188
17.877
11.059
1.344
1.00
30.31
O


ATOM
6026
O
HOH
W
189
1.036
−11.099
25.828
1.00
27.54
O


ATOM
6029
O
HOH
W
190
19.608
24.676
5.693
1.00
30.78
O


ATOM
6032
O
HOH
W
191
19.946
19.409
42.111
1.00
26.14
O


ATOM
6035
O
HOH
W
192
3.476
10.629
29.248
1.00
33.62
O


ATOM
6038
O
HOH
W
193
30.257
27.659
31.979
1.00
37.42
O


ATOM
6041
O
HOH
W
194
16.442
32.431
6.611
1.00
24.66
O


ATOM
6044
O
HOH
W
195
34.073
12.318
2.203
1.00
35.07
O


ATOM
6047
O
HOH
W
196
4.395
17.038
28.248
1.00
26.36
O


ATOM
6050
O
HOH
W
197
33.318
3.825
31.828
1.00
23.35
O


ATOM
6053
O
HOH
W
198
18.983
33.274
37.816
1.00
19.94
O


ATOM
6056
O
HOH
W
199
13.726
12.394
40.361
1.00
26.01
O


ATOM
6059
O
HOH
W
200
12.010
7.108
2.281
1.00
28.49
O


ATOM
6062
O
HOH
W
201
17.870
4.803
31.837
1.00
28.97
O


ATOM
6065
O
HOH
W
202
27.323
19.119
43.497
1.00
31.89
O


ATOM
6068
O
HOH
W
203
24.085
33.024
8.818
1.00
27.59
O


ATOM
6071
O
HOH
W
204
19.302
−7.180
37.095
1.00
25.01
O


ATOM
6074
O
HOH
W
205
34.921
3.097
15.744
1.00
35.90
O


ATOM
6077
O
HOH
W
206
22.046
36.365
19.883
1.00
33.71
O


ATOM
6080
O
HOH
W
207
4.178
27.080
39.663
1.00
28.68
O


ATOM
6083
O
HOH
W
208
21.450
25.719
2.335
1.00
29.50
O


ATOM
6086
O
HOH
W
209
7.625
37.543
22.457
1.00
27.39
O


ATOM
6089
O
HOH
W
210
27.905
0.363
30.805
1.00
27.13
O


ATOM
6092
O
HOH
W
211
29.963
26.817
34.574
1.00
28.33
O


ATOM
6095
O
HOH
W
212
37.812
29.413
15.021
1.00
35.13
O


ATOM
6098
O
HOH
W
213
31.600
5.299
33.905
1.00
30.36
O


ATOM
6101
O
HOH
W
214
0.934
2.893
30.953
1.00
26.27
O


ATOM
6104
O
HOH
W
215
15.151
−19.091
27.503
1.00
36.44
O


ATOM
6107
O
HOH
W
216
31.891
29.580
32.140
1.00
23.31
O


ATOM
6110
O
HOH
W
217
13.828
−7.788
38.638
1.00
33.17
O


ATOM
6113
O
HOH
W
218
37.026
8.221
8.178
1.00
43.15
O


ATOM
6116
O
HOH
W
219
12.026
−5.374
16.938
1.00
29.86
O


ATOM
6119
O
HOH
W
220
−1.767
−3.163
19.781
1.00
23.61
O


ATOM
6122
O
HOH
W
221
5.748
3.990
37.161
1.00
34.03
O


ATOM
6125
O
HOH
W
222
15.126
10.026
2.394
1.00
29.52
O


ATOM
6128
O
HOH
W
223
28.930
25.732
2.063
1.00
32.92
O


ATOM
6131
O
HOH
W
224
17.834
38.165
18.660
1.00
32.25
O


ATOM
6134
O
HOH
W
225
15.576
−9.633
19.956
1.00
29.35
O


ATOM
6137
O
HOH
W
226
21.532
33.500
36.344
1.00
30.91
O


ATOM
6140
O
HOH
W
227
37.166
25.308
14.969
1.00
30.80
O


ATOM
6143
O
HOH
W
228
4.201
13.978
20.632
1.00
29.14
O


ATOM
6146
O
HOH
W
229
−8.921
0.073
20.951
1.00
29.35
O


ATOM
6149
O
HOH
W
230
30.930
14.280
0.673
1.00
42.54
O


ATOM
6152
O
HOH
W
231
0.993
−10.363
23.294
1.00
35.64
O


ATOM
6155
O
HOH
W
232
19.283
−9.456
35.875
1.00
21.72
O


ATOM
6158
O
HOH
W
233
29.715
33.139
9.438
1.00
28.66
O


ATOM
6161
O
HOH
W
234
2.904
−7.322
21.953
1.00
29.37
O


ATOM
6164
O
HOH
W
235
−0.395
23.877
34.029
1.00
37.81
O


ATOM
6167
O
HOH
W
236
15.054
−3.907
38.561
1.00
26.91
O


ATOM
6170
O
HOH
W
237
25.729
34.682
11.457
1.00
29.23
O


ATOM
6173
O
HOH
W
238
9.385
33.323
24.057
1.00
36.55
O


ATOM
6176
O
HOH
W
239
24.093
−5.021
21.077
1.00
26.10
O


ATOM
6179
O
HOH
W
240
34.767
17.185
37.911
1.00
29.03
O


ATOM
6182
O
HOH
W
241
18.069
25.299
24.326
1.00
23.95
O


ATOM
6185
O
HOH
W
242
25.539
23.840
−0.133
1.00
27.32
O


ATOM
6188
O
HOH
W
243
−8.581
−0.882
24.380
1.00
32.46
O


ATOM
6191
O
HOH
W
244
37.140
34.955
20.025
1.00
39.20
O


ATOM
6194
O
HOH
W
245
25.828
−6.464
17.951
1.00
35.90
O


ATOM
6197
O
HOH
W
246
20.526
5.042
2.568
1.00
23.17
O


ATOM
6200
O
HOH
W
247
16.909
37.789
30.355
1.00
24.49
O


ATOM
6203
O
HOH
W
248
4.170
−13.753
24.179
1.00
34.75
O


ATOM
6206
O
HOH
W
249
4.757
29.554
36.890
1.00
27.07
O


ATOM
6209
O
HOH
W
250
14.985
25.383
44.611
1.00
36.67
O


ATOM
6212
O
HOH
W
251
21.002
34.942
26.743
1.00
24.40
O


ATOM
6215
O
HOH
W
252
35.187
37.614
16.171
1.00
41.70
O


ATOM
6218
O
HOH
W
253
9.429
35.849
24.299
1.00
29.47
O


ATOM
6221
O
HOH
W
254
22.360
−8.508
14.886
1.00
39.42
O


ATOM
6224
O
HOH
W
255
27.125
28.229
2.829
1.00
36.93
O


ATOM
6227
O
HOH
W
256
7.686
9.225
33.089
1.00
35.16
O


ATOM
6230
O
HOH
W
257
4.744
8.641
8.479
1.00
31.36
O


ATOM
6233
O
HOH
W
258
43.322
15.064
19.229
1.00
34.65
O


ATOM
6236
O
HOH
W
259
12.158
34.202
31.572
1.00
22.62
O


ATOM
6239
O
HOH
W
260
40.415
22.262
16.091
1.00
26.05
O


ATOM
6242
O
HOH
W
261
7.689
33.643
34.608
1.00
27.52
O


ATOM
6245
O
HOH
W
262
−2.516
−11.500
29.608
1.00
27.56
O


ATOM
6248
O
HOH
W
263
23.197
30.603
38.577
1.00
30.81
O


ATOM
6251
O
HOH
W
264
1.669
−4.135
18.399
1.00
34.72
O


ATOM
6254
O
HOH
W
265
31.682
18.313
2.510
1.00
27.38
O


ATOM
6257
O
HOH
W
266
21.515
33.588
40.094
1.00
30.03
O


ATOM
6260
O
HOH
W
267
16.458
13.271
−0.901
1.00
38.54
O


ATOM
6263
O
HOH
W
268
40.177
32.128
16.843
1.00
41.26
O


ATOM
6266
O
HOH
W
269
12.143
−2.734
15.885
1.00
27.02
O


ATOM
6269
O
HOH
W
270
27.486
−3.196
12.318
1.00
30.88
O


ATOM
6272
O
HOH
W
271
15.668
6.307
39.384
1.00
31.07
O


ATOM
6275
O
HOH
W
272
7.819
6.715
30.569
1.00
22.48
O


ATOM
6278
O
HOH
W
273
29.983
17.529
41.917
1.00
33.57
O


ATOM
6281
O
HOH
W
274
2.674
6.648
20.688
1.00
34.77
O


ATOM
6284
O
HOH
W
275
16.983
2.502
33.738
1.00
29.85
O


ATOM
6287
O
HOH
W
276
18.800
36.320
34.162
1.00
30.58
O


ATOM
6290
O
HOH
W
277
12.363
24.605
−1.596
1.00
34.55
O


ATOM
6293
O
HOH
W
278
14.702
17.110
−5.593
1.00
27.29
O


ATOM
6296
O
HOH
W
279
40.591
12.389
6.204
1.00
33.96
O


ATOM
6299
O
HOH
W
280
31.608
16.687
1.057
1.00
38.88
O


ATOM
6302
O
HOH
W
281
23.897
−11.952
20.222
1.00
34.68
O


ATOM
6305
O
HOH
W
282
11.219
39.478
21.517
1.00
33.54
O


ATOM
6308
O
HOH
W
283
2.552
16.703
24.563
1.00
35.86
O


ATOM
6311
O
HOH
W
284
27.258
9.495
42.694
1.00
30.97
O


ATOM
6314
O
HOH
W
285
5.535
8.881
16.549
1.00
28.65
O


ATOM
6317
O
HOH
W
286
2.189
24.192
36.099
1.00
31.47
O


ATOM
6320
O
HOH
W
287
19.058
−1.798
12.329
1.00
34.23
O


ATOM
6323
O
HOH
W
288
10.635
34.933
33.408
1.00
30.86
O


ATOM
6326
O
HOH
W
289
4.333
−0.576
36.893
1.00
44.65
O


ATOM
6329
O
HOH
W
290
25.069
21.389
−1.195
1.00
37.85
O


ATOM
6332
O
HOH
W
291
28.073
7.826
39.103
1.00
28.63
O


ATOM
6335
O
HOH
W
292
14.225
35.260
33.101
1.00
43.65
O


ATOM
6338
O
HOH
W
293
18.965
−2.916
15.137
1.00
38.32
O


ATOM
6341
O
HOH
W
294
40.370
19.000
13.716
1.00
33.50
O


ATOM
6344
O
HOH
W
295
6.261
32.033
33.639
1.00
30.04
O


ATOM
6347
O
HOH
W
296
13.696
37.943
17.160
1.00
36.07
O


ATOM
6350
O
HOH
W
297
2.518
21.734
35.950
1.00
40.41
O


ATOM
6353
O
HOH
W
298
31.821
26.389
38.109
1.00
33.84
O


ATOM
6356
O
HOH
W
299
−1.406
2.015
20.824
1.00
32.14
O


ATOM
6359
O
HOH
W
300
27.841
5.928
34.623
1.00
31.37
O


ATOM
6362
O
HOH
W
301
33.128
25.346
29.949
1.00
26.11
O


ATOM
6365
O
HOH
W
302
16.952
35.715
12.956
1.00
35.08
O


ATOM
6368
O
HOH
W
303
19.607
39.276
19.928
1.00
43.31
O


ATOM
6371
O
HOH
W
304
31.667
24.286
33.803
1.00
31.06
O


ATOM
6374
O
HOH
W
305
9.682
34.631
35.657
1.00
36.70
O


ATOM
6377
O
HOH
W
306
24.913
37.958
16.991
1.00
33.31
O


ATOM
6380
O
HOH
W
307
27.526
−1.443
8.577
1.00
31.08
O


ATOM
6383
O
HOH
W
308
34.923
14.237
35.665
1.00
33.29
O


ATOM
6386
O
HOH
W
309
23.480
3.819
33.037
1.00
28.70
O


ATOM
6389
O
HOH
W
310
39.917
30.468
13.351
1.00
43.99
O


ATOM
6392
O
HOH
W
311
20.005
30.742
39.713
1.00
31.34
O


ATOM
6395
O
HOH
W
312
1.762
19.900
10.728
1.00
33.53
O


ATOM
6398
O
HOH
W
313
21.282
35.887
11.600
1.00
34.66
O


ATOM
6401
O
HOH
W
314
22.512
−2.583
9.740
1.00
30.42
O


ATOM
6404
O
HOH
W
315
19.079
3.993
34.105
1.00
40.61
O


ATOM
6407
O
HOH
W
316
2.068
20.663
16.198
1.00
29.05
O


ATOM
6410
O
HOH
W
317
2.691
6.046
36.126
1.00
41.50
O


ATOM
6413
O
HOH
W
318
34.645
5.648
8.339
1.00
37.23
O


ATOM
6416
O
HOH
W
319
23.607
9.100
43.132
1.00
28.98
O


ATOM
6419
O
HOH
W
320
32.041
25.470
4.056
1.00
37.21
O


ATOM
6422
O
HOH
W
321
20.362
8.689
26.048
1.00
25.83
O


ATOM
6425
O
HOH
W
322
11.708
6.948
24.652
1.00
11.83
O


ATOM
6428
O
HOH
W
323
27.069
1.261
5.299
1.00
22.09
O


ATOM
6431
O
HOH
W
324
23.654
25.543
41.612
1.00
22.43
O


ATOM
6434
O
HOH
W
325
23.776
33.886
17.441
1.00
26.34
O


ATOM
6437
O
HOH
W
326
34.498
18.045
27.924
1.00
26.83
O


ATOM
6440
O
HOH
W
327
34.129
9.041
28.001
1.00
29.40
O


ATOM
6443
O
HOH
W
328
22.398
−4.833
18.306
1.00
31.97
O


ATOM
6446
O
HOH
W
329
32.650
36.061
13.569
1.00
32.16
O


ATOM
6449
O
HOH
W
330
18.875
6.699
27.476
1.00
31.00
O


ATOM
6452
O
HOH
W
331
43.627
18.936
5.210
1.00
33.15
O


ATOM
6455
O
HOH
W
332
13.390
23.630
−3.991
1.00
34.31
O


ATOM
6458
O
HOH
W
333
−0.102
6.438
34.964
1.00
48.55
O


ATOM
6461
O
HOH
W
334
−0.118
6.687
37.269
1.00
35.34
O


ATOM
6464
O
HOH
W
335
37.771
7.069
11.352
1.00
32.60
O


ATOM
6467
O
HOH
W
336
31.257
24.829
41.652
1.00
34.05
O


ATOM
6470
O
HOH
W
337
10.129
21.939
40.735
1.00
34.24
O


ATOM
6473
O
HOH
W
338
6.286
34.026
36.723
1.00
34.35
O


ATOM
6476
O
HOH
W
339
10.691
33.573
28.674
1.00
35.22
O


ATOM
6479
O
HOH
W
340
12.399
2.285
25.575
1.00
35.31
O


ATOM
6482
O
HOH
W
341
34.307
9.058
2.642
1.00
42.37
O


ATOM
6485
O
HOH
W
342
15.597
2.822
28.675
1.00
34.26
O


ATOM
6488
O
HOH
W
343
11.007
37.789
24.517
1.00
39.01
O


ATOM
6491
O
HOH
W
344
6.436
−13.362
22.903
1.00
41.47
O


ATOM
6494
O
HOH
W
345
19.680
17.126
45.857
1.00
37.81
O


ATOM
6497
O
HOH
W
346
10.533
36.113
27.412
1.00
34.50
O


ATOM
6500
O
HOH
W
347
41.809
19.887
16.869
1.00
37.61
O


ATOM
6503
O
HOH
W
348
21.611
15.053
−0.998
1.00
45.55
O


ATOM
6506
O
HOH
W
349
22.337
−9.031
17.861
1.00
49.31
O


ATOM
6509
O
HOH
W
350
9.303
25.984
−1.560
1.00
39.69
O


ATOM
6512
O
HOH
W
351
13.153
3.923
27.389
1.00
35.74
O


ATOM
6515
O
HOH
W
352
20.365
4.572
37.533
1.00
43.93
O


ATOM
6518
O
HOH
W
353
−2.246
27.322
10.724
1.00
42.74
O


ATOM
6521
O
HOH
W
354
9.435
33.000
4.908
1.00
38.59
O


ATOM
6524
O
HOH
W
355
15.473
17.843
44.371
1.00
40.00
O


ATOM
6527
O
HOH
W
356
13.009
31.258
32.197
1.00
21.10
O


ATOM
6530
O
HOH
W
357
36.838
8.242
3.607
1.00
35.04
O


ATOM
6533
O
HOH
W
358
30.674
−0.077
10.899
1.00
34.69
O


ATOM
6536
O
HOH
W
359
31.146
37.498
19.980
1.00
36.95
O


ATOM
6539
O
HOH
W
360
16.880
−0.664
8.461
1.00
39.07
O


ATOM
6542
O
HOH
W
361
40.707
6.845
20.023
1.00
32.40
O


ATOM
6545
O
HOH
W
362
19.502
25.524
0.606
1.00
41.49
O


ATOM
6548
O
HOH
W
363
27.574
22.139
44.653
1.00
52.17
O


ATOM
6551
O
HOH
W
364
11.308
11.015
41.537
1.00
41.08
O


ATOM
6554
O
HOH
W
365
9.385
−15.147
33.423
1.00
33.24
O


ATOM
6557
O
HOH
W
366
8.340
36.413
6.953
1.00
46.38
O


ATOM
6560
O
HOH
W
367
1.749
16.224
29.518
1.00
42.28
O


ATOM
6563
O
HOH
W
368
21.762
29.936
41.718
1.00
39.52
O


ATOM
6566
O
HOH
W
369
5.916
6.084
8.101
1.00
42.08
O


ATOM
6569
O
HOH
W
370
22.021
34.841
24.376
1.00
41.54
O


ATOM
6572
O
HOH
W
371
21.487
−18.393
21.594
1.00
38.90
O


ATOM
6575
O
HOH
W
372
39.073
5.237
9.752
1.00
40.75
O


ATOM
6578
O
HOH
W
373
23.013
−4.111
6.967
1.00
39.88
O


ATOM
6581
O
HOH
W
374
14.536
36.281
12.246
1.00
37.61
O


ATOM
6584
O
HOH
W
375
29.859
34.515
20.996
1.00
28.10
O


ATOM
6587
O
HOH
W
376
28.570
38.564
14.823
1.00
51.95
O


ATOM
6590
O
HOH
W
377
33.330
21.383
4.315
1.00
36.55
O


ATOM
6593
O
HOH
W
378
44.550
14.418
11.401
1.00
45.79
O


ATOM
6596
O
HOH
W
379
20.051
17.411
−1.528
1.00
37.86
O


ATOM
6599
O
HOH
W
380
0.588
25.706
15.094
1.00
42.46
O


ATOM
6602
O
HOH
W
381
4.339
−3.887
18.462
1.00
42.68
O


ATOM
6605
O
HOH
W
382
15.219
38.779
25.987
1.00
45.72
O


ATOM
6608
O
HOH
W
383
26.263
−1.807
0.657
1.00
35.43
O


ATOM
6611
O
HOH
W
384
43.222
8.889
13.058
1.00
39.12
O


ATOM
6614
O
HOH
W
385
3.804
15.072
37.146
1.00
35.83
O


ATOM
6617
O
HOH
W
386
13.685
22.152
44.161
1.00
46.34
O


ATOM
6620
O
HOH
W
387
39.745
6.714
14.451
1.00
43.87
O


ATOM
6623
O
HOH
W
388
4.160
10.246
14.729
1.00
40.70
O


ATOM
6626
O
HOH
W
389
8.951
3.749
5.381
1.00
35.43
O


ATOM
6629
O
HOH
W
390
12.861
−18.451
24.960
1.00
37.73
O


ATOM
6632
O
HOH
W
391
2.829
15.459
20.671
1.00
39.80
O


ATOM
6635
O
HOH
W
392
13.542
1.619
37.956
1.00
42.36
O


ATOM
6638
O
HOH
W
393
16.727
31.361
39.312
1.00
38.23
O


ATOM
6641
O
HOH
W
394
36.063
3.510
12.324
1.00
41.15
O


ATOM
6644
O
HOH
W
395
5.800
1.387
38.322
1.00
38.68
O


ATOM
6647
O
HOH
W
396
12.445
36.715
28.596
1.00
36.10
O


ATOM
6650
O
HOH
W
397
2.782
12.760
27.641
1.00
47.29
O


ATOM
6653
O
HOH
W
398
−1.700
−3.625
37.395
1.00
36.77
O


ATOM
6656
O
HOH
W
399
41.093
10.367
21.318
1.00
46.59
O


ATOM
6659
O
HOH
W
400
21.734
−5.069
9.760
1.00
48.11
O


ATOM
6662
O
HOH
W
401
14.290
3.253
24.475
1.00
36.49
O


ATOM
6665
O
HOH
W
402
22.729
2.974
−0.954
1.00
40.03
O


ATOM
6668
O
HOH
W
403
4.597
11.469
0.380
1.00
42.88
O


ATOM
6671
O
HOH
W
404
17.898
4.552
−0.927
1.00
39.85
O

## Claims

1. A subtilase variant, lacking one, two or three calcium ion binding sites due to a deletion of an amino acid residue at one or more positions corresponding to positions 212-224 of SEQ ID NO: 1, wherein the subtilase variant is at least 90% identical to the sequence of SEQ ID NO:1 and has subtilase activity.

2. The subtilase variant of claim 1, which is at least 92% identical to the sequence of SEQ ID NO: 1.

3. The subtilase variant of claim 1, which is at least 94% identical to the sequence of SEQ ID NO: 1.

4. The subtilase variant of claim 1, which is at least 96% identical to the sequence of SEQ ID NO: 1.

5. The subtilase variant of claim 1, which is at least 98% identical to the sequence of SEQ ID NO: 1.

6. The subtilase variant of claim 1, which is at least 99% identical to the sequence of SEQ ID NO: 1.

7. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 212 of SEQ ID NO: 1.

8. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 213 of SEQ ID NO: 1.

9. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 214 of SEQ ID NO: 1.

10. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 215 of SEQ ID NO: 1.

11. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 216 of SEQ ID NO: 1.

12. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 217 of SEQ ID NO: 1.

13. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 218 of SEQ ID NO: 1.

14. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 219 of SEQ ID NO: 1.

15. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 220 of SEQ ID NO: 1.

16. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 221 of SEQ ID NO: 1.

17. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 222 of SEQ ID NO: 1.

18. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 223 of SEQ ID NO: 1.

19. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at a position corresponding to position 224 of SEQ ID NO: 1.

20. The subtilase variant of claim 1, which comprises a deletion of the amino acid residue at positions corresponding to positions 212-224 of SEQ ID NO: 1.

21. The subtilase variant of claim 1, which further comprises an alteration at one or more positions corresponding to positions 84, 85, 86, 87, 88, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 141, 142, 143, 144, 145, 146, 150, 151, 152, 169, 170, 171, 200, 201, 211, 242, 243, 268, 269 and 270 of SEQ ID NO: 1.

22. A detergent composition comprising a subtilase variant of claim 1 and a surfactant.