Food Safety and Inspection Service 

Annual Sampling Program Plan 

Fiscal Year 2022 

United States Department of Agriculture 

Food Safety and Inspection Service 

1 
 

 

 

I 

Contents 

Introduction..................................................................................................................................................3 

Background ...............................................................................................................................................3 

Table 1: Planned Number of Microbiological Analyses (tests) and Analytes FY 2019-FY 2021 ....................5 

Table 2: Planned Number of Chemical Residue Analyses and Analytes Reported FY 2019-FY 2021.............6 

Significant Changes for the FY 2021 Plan ........................................................................................................7 

Table 3: FY 2021 Sampling Priorities..........................................................................................................7 

Table 4: Rationale for Changes in Sampling Allocations.............................................................................8 

Appendix A: Microbial Sampling Numbers by Product ..................................................................................13 

Table A1: Summary of Analyte Tested by Product...................................................................................13 

Table A2: FY 2020 and FY 2021 Sample Numbers for Raw Beef ................................................................14 

Table A3: FY 2020 and FY 2021 Sample Numbers for Raw Pork ................................................................15 

Table A4: FY 2020 and FY 2021 Sample Numbers for Raw Siluriformes ....................................................15 

Table A5: FY 2020 and FY 2021 Sample Numbers for Raw Poultry............................................................15 

Appendix B: Chemical Residue Sampling Numbers by Product......................................................................18 

Table B1: FY 2019 and FY 2020 Sample Numbers for Chemical Residues ..................................................18 

Appendix C: National Antimicrobial Resistance Monitoring System (NARMS) Programs.................................23 

Table C1: FY 2020 and FY 2021 Sample Numbers for NARMS ...................................................................23 

Appendix D: Other Sampling Programs ........................................................................................................25 

Table D1: FY 2020 and FY 2021 Sample Numbers for FSIS Sampling Programs Other than Microbiological 

and Chemical Residues ............................................................................................................................25 

Appendix E: Terms, Definitions and References............................................................................................26 

References..................................................................................................................................................27 

2 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Introduction 

The Food Safety and Inspection Service (FSIS) is the food safety regulatory agency within the United States 

Department of Agriculture (USDA) responsible for ensuring that domestic and imported meat, poultry, and 

egg products are safe, wholesome, and accurately labeled. Verification activities serve to protect the public 

from foodborne hazards. A key FSIS inspection verification activity is the sampling and testing of product 

for microbiological contaminants or chemical residues. 

This report identifies changes planned for fiscal year (FY) 2022 to FSIS’ various sampling programs and 

describes the Agency’s overall strategy for directing its sampling resources. 

Background 

FSIS Agency Planning 

The FSIS Strategic Plan for FY 2017-2021 includes an objective to strengthen FSIS sampling programs. The 

planned FY 2022-2026 Strategic Plan and FY 2022 Annual Plan will continue to ensure that sampling 

allocation remains aligned with Agency goals. 

FSIS Process for Scheduling, Collecting, and Analyzing Samples 

The Agency’s process of scheduling, collecting, and analyzing routine domestic samples typically begins with 

a sampling task assigned to FSIS inspection program personnel(IPP) through the Public Health Information 

System (PHIS). The number of sampling tasks IPP can receive at a domestic establishment varies greatly 

depending on the types and quantities of products produced. Additional non-routine sampling tasks might 

be assigned to an establishment, or country for imported product sampling, in response to routine results or 

other establishment performance history. Sampling type of inspection (TOI) tasks are assigned to imported 

product from each foreign country and product combination based on the number of imported shipments 

received. 

It is important to note there might be a difference between the number of samples that are anticipated to 

be analyzed and the total number of samples analyzed within the fiscal year. The lack of available products 

that are eligible for a specific sampling project within the specific sampling tasks’ allotted timeframe is one 

of the biggest challenges IPP face when trying to collect all the samples accounted for in the sampling plan; 

therefore, the FSIS Annual Sampling Plan is based on the number of samples anticipated to be analyzed 

instead of those assigned. For those projects that do not have a required monthly frequency, FSIS can adjust 

the number of samples assigned throughout the year to reach the sample target numbers. Additionally, 

differences between the planned number and analyzed number of samples may be due to changes in the 

number of inspected establishments producing eligible products. To collect samples from infrequent 

producers and optimize the total number of annual planned samples collected and analyzed, FSIS adjusts 

3 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

the number of samples assigned based on the average number of samples collected throughout the 

sampling year. The estimates for each sampling program are based on current plans, FSIS policies, and 

industry practices, and are subject to change over the course of the fiscal year. 

After receiving the sampling tasks and verifying eligible product availability, IPP collect and ship the samples 

to one of three FSIS testing laboratories, where the sample is tested for specified analytes. An analyte is a 

substance whose constituents are identified and measured, and the FSIS laboratories perform different tests 

depending on the sampling program and target analytes. The Agency increases sample resource efficiency 

by maximizing the number of analytes evaluated per sample collection and test. 

Data Sharing and Analysis 

FSIS routinely analyzes sampling data. The results of these analyses are used in a variety of ways, including 

verifying whether product is safe and not adulterated; monitoring the effectiveness, where applicable, of 

Hazard Analysis and Critical Control Point systems; informing Agency policy making; estimating public health 

impact; and advising strategic and performance planning. FSIS posts most of the sampling data on the 

Agency’s website and shares the data with establishments through quarterly letters, as well as directly 

sharing sampling results with IPP and establishments. 

FSIS engages with Federal partners to use whole genome sequencing (WGS) data for regulatory and public 

health purposes. FSIS aligns WGS-related projects with the goals and objectives of the FSIS Strategic Plan and 

other policies. FSIS laboratories perform WGS on all pathogens isolated from FSIS-regulated products. The 

information gathered from WGS helps FSIS to detect and investigate outbreaks of foodborne illness, identify 

potential instances of harborage, and identify unique genes, including antimicrobial resistance genes. In FY 

2020, FSIS modernized Salmonella serotyping by using WGS data to determine the serotype (FSIS 

Constituent Update, Dec. 6, 2019). This update created efficiencies within the Agency by reducing the 

number of analyses required to determine the same, if not more, information. Moving forward, FSIS will 

explore new ways to expand the use of WGS data to support the regulatory and public health efforts of FSIS 

more effectively. Potential future efforts include exploring how to use genomic data to assess pathogen 

adaptability and persistence as well as the potential for pathogenicity and virulence of Salmonella. These 

efforts will build on public health, regulatory, and research partners’ efforts in support of FSIS Research 

Priorities. 

4 
 

 

 

 

 

 

 

 

Microbiological and Chemical Residue Sampling Planned Changes from FY 2020 to FY 2022 

Table 1 and Table 2 summarize microbiological and chemical residue programs, respectively, the total planned number of analyses and 

corresponding planned number of analytes tested for during FY 2020, FY 2021, and FY 2022 by product class. Data is based on the proposed 

number of samples and actual analyses performed during the previous fiscal years. Results for the fiscal year can be found in FSIS’ Annual 

Sampling Summary Report. A link to past years’ reports can be found in the References section. 

Table 1: Planned Number of Microbiological Analyses (Tests) and Analytes FY 2020-FY 2022 

Difference1 

Planned for FY 2020 Planned for FY 2021 Planned for FY 2022 (FY 2022-FY2021) 

Samples Tests Analytes Samples Tests Analytes Samples Tests Analytes Samples Tests Analytes 

Product Class Planned Planned Planned Planned Planned Planned Planned Planned Planned Planned Planned Planned 

Raw Beef 18,762 49,416 94,488 19,233 51,240 99,336 19,999 54,456 112,632 766 3,216 13,296 

Raw Pork 11,040 22,080 33,120 11,040 22,080 33,120 11,040 22,080 33,120 0 0 0 

Raw Poultry 47,736 64,416 64,416 47,892 64,248 64,248 47,892 64,248 64,248 0 0 0 

Raw 660 660 660 660 660 660 660 660 660 0 0 0 Siluriformes 

RTE 15,919 29,616 29,616 15,919 29,616 29,616 15,919 29,616 29,616 0 0 0 

RLm 5,437 5,437 5,437 5,437 5,437 5,437 5,437 5,437 5,437 0 0 0 

Eggs 1,600 3,200 3,200 1,600 3,200 3,200 1,600 3,200 3,200 0 0 0 

NARMS 7,780 18,600 443,100 7,780 18,600 443,100 7,480 17,700 412,144 -300 -900 -30,956 RTE/Eggs 

Imports2 6,804 14,976 29,376 6,312 13,884 27,893 6,312 13,884 27,893 0 0 0 

Total 115,738 208,391 703,413 115,873 208,616 706,741 116,339 211,281 688,950 466 2,316 -17,660 

Abbreviations: RTE, ready-to-eat; RLm, Routine Listeriamonocytogenesmonitoring; NARMS, National Antimicrobial Resistance Monitoring System. 1 The differences betweenFY 2021and FY 2022 plans includeadditional sampling forestablishments selected for exploratory sampling, and cessation of the NARMS 

mesenteric lymph node sampling programs. For a full list of allocation changes, please seeTable 4. 2 Import microbiology testing analyses estimates are driven by expected shipment frequency and volume-based TOI assignments. 

5 
 

 

 

 

Table 2: Planned Number of Chemical Residue Analyses and Analytes Reported FY 2020-FY 2022 

Planned for FY 2020 Planned for FY 2021 Planned for FY 2022 Difference3 (FY 2022-FY2021) 

Product Class 

Samples 

Planned 

Tests 

Planned 

Analytes 

Planned 

Samples 

Planned 

Tests 

Planned 

Analytes 

Planned 

Samples 

Planned 

Tests 

Planned 

Analytes 

Planned 

Samples 

Planned 

Tests 

Planned 

Analytes 

Planned 

Domestic Residues 

Beef Cows 712 3,240 121,320 752 2,646 132,678 752 2,268 130,788 0 -378 -1,890 

Bob Veal 356 1,620 60,660 400 1,428 71,604 400 1,224 70,584 0 -204 -1,020 

Dairy Cows 712 3,240 121,320 788 3,152 144,992 788 2,376 137,016 0 -776 -7,976 

Heifers 356 1,620 60,660 340 1,218 61,074 340 1,044 60,204 0 -174 -870 

Steer 356 1,620 60,660 328 1,176 58,968 328 1,008 58,128 0 -168 -840 

Sows 712 2,160 111,600 788 3,152 144,992 788 2,772 143,748 0 -380 -1,244 

Market Swine 712 2,880 119,880 728 2,912 133,952 728 2,562 132,858 0 -350 -1,094 

Young Chickens 356 1,030 55,440 394 1,182 53,190 394 1,584 72,270 0 402 19,080 

Whole Chickens 356 1,030 55,440 394 1,576 90,620 394 1,584 72,270 0 8 -18,350 

Young Turkeys 712 2,160 110,880 788 2,772 137,808 788 2,772 137,808 0 0 0 

Sheep 100 357 16,728 100 300 16,728 100 300 16,728 0 0 0 

Lamb 100 357 16,728 100 300 16,728 100 300 16,728 0 0 0 

Goats 300 900 35,100 300 600 35,100 300 600 35,100 0 0 0 

Roaster Swine 300 300 300 300 150 150 300 150 150 0 0 0 

Veal - Other 150 640 19,890 150 588 29,484 150 504 29,064 0 -84 -420 

Egg Product 250 500 37,296 250 500 38,637 250 504 39,139 0 4 502 

Siluriformes 650 2,130 103,290 650 2,455 116,075 650 2,640 116,160 0 185 85 

State-Inspected 

Establishment Sampling 

for U.S. National Residue 720 2,760 128,208 300 1,050 52,614 300 960 52,740 0 -90 126 

Program1 

Other 

2

Imports 4,000 20,076 773,892 3,400 18,276 675,805 3,400 17,688 689,857 0 -588 14,052 

KISTM 4,000 8,000 424,000 4,000 8,000 468,936 4,000 8,000 468,936 0 0 0 

Total 15,910 56,620 2,433,292 15,250 53,433 2,480,135 15,250 50,840 2,480,276 0 -2593 141 

Abbreviation: KISTM, Kidney Inhibition Swab. 1 State sampling adjusted to reflect number of eligibleState establishments. 2 Importresidue testing analysesestimates are driven by expected shipment frequency and volume-basedTOI assignments. 3 The differences between the FY 2021and FY 2022 plans include:Replacing the stand-alone B-agonist screen with the updated multiresidue method (MRM) reduced the 

number of tests needed and resulting analytes from that additional test because B-agonist testing is now included in the updated MRM method. 

6 
 

 

 

 

 

 

 

Significant Changes for the FY 2022 Plan 

Table 3 lists key priorities FSIS plans to implement in FY 2022. Each row describes the challenges that the Agency faces moving into FY 2022, what 

process is impacted and the objective(s) to achieve during the fiscal year. This table will also include modifications that may have taken place 

during FY 2021 after the FY 2021 Plan was published. 

Table 3: FY 2022 Sampling Priorities 

FY 2022 Modification 

Impacted Sampling, 

Related Process, or 

Analyte Description of Modification Implemented 

Changes to the National Residue Program (NRP) Beta agonist method • Improvements to the multi-residue veterinary drug method 

(MRM) now include all analytes normally screened in the standalone Beta agonist method. FSIS will replace the stand-along Bagonist screening method with the MRM method. 

Availability of new rapid pathogen enumeration 

methods 

Pathogen 

Enumeration 

• FSIS will evaluate newly available, rapid enumeration methods. 

7 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Table 4 lists each sampling program and includes a description of that program, whether changes were made to sampling allocations for each 

program, and the Agency’s reasoning for the changes. The rationale is included for sampling number allocations changes between FY 2021 and FY 

2022 sampling plans. Some sampling program allocations did not change as the Agency continues to verify these commodities to ensure effective 

and efficient food safety verification to protect consumers. There are changes under consideration not noted within this plan because they are 

going through governance. These changes will be announced throughout the year separately as they become approved. 

Table 4: Rationale for Changes in Sampling Allocations 

Sampling by 

Program/Commodity Program Description 

Rationale for Any Changes from the FY 2021 Sampling 

Allocations 

Beef Products • FSIS conducts Shiga toxin-producing E. coli (STEC) 

sampling for product produced in domestic 

establishments, imported products, and retail. 

• Raw non-intact beef products and raw beef 

products intended for raw non-intact use are eligible 

for sampling, including ground beef, bench trim, 

beef manufactured trimmings, and other raw ground 

beef components. 

• FSIS analyzes all raw beef products collected under 

the routine and follow-up sampling programs, 

including raw ground beef, bench trim, beef 

manufactured trimmings, and other raw ground beef 

components for E. coli O157:H7 and Salmonella. 

Additionally, FSIS analyzes beef manufacturing 

trimmings for non-O157 STEC as well. 

• Increased allocations for exploratory beef carcass 

sampling (pre- and post-evisceration) by 208 samples 

due to additional establishment(s) selected for 

exploratory project. 

• Expand non-O157 STEC analysis to all raw beef 

products analyzed for E. coli O157:H7 

• Continue to collect MT60_CLOTH samples and 

evaluate the cloth surface sampling method compared 

to N60 pathogen detection while conducting an in-lab 

study to optimize recovery using the cloth. 

• Investigate options for enumeration of positive E. coli 

O157:H7 and Salmonella samples. 

Pork Products • FSIS analyzes raw intact, non-intact, and 

comminuted domestic and imported pork for 

Salmonella. 

• No allocation changes planned for FY 2022. 

• Investigate the options for enumeration of positive 

Salmonella samples. 

8 
 

 

 

 

 

 

 

 

Sampling by 

Program/Commodity Program Description 

Rationale for Any Changes from the FY 2021 Sampling 

Allocations 

Poultry Products • FSIS analyzes young chicken and turkey carcasses, 

comminuted chicken and turkey, and chicken parts 

samples for Salmonella and Campylobacter. 

• No allocation changes planned at this time. 

• Evaluate options for enumeration of samples positive 

for Salmonella and Campylobacter. 

Siluriformes • FSIS analyzes raw fish of the order Siluriformes for 

Salmonella. 

• No allocation changes planned for FY 2022. 

Ready-To-Eat (RTE): Meat, • FSIS conducts microbiological testing for Listeria • Investigate the options for enumeration of positive Lm 

Poultry, and Egg Products monocytogenes (Lm) and Salmonella in both 

domestically produced and imported egg products in 

addition to other RTE products. 

• Product sampling is scheduled every month under 

random sampling and risk-based sampling projects 

under 2 RTEPROD projects. 

• RLm sampling program is performed in 

establishments producing post-lethality exposed RTE 

product. An RLm sampling event includes samples, 

consisting of product, contact surfaces, and the 

processing environment, collected and sampled for 

Lm under 3 RLm project codes. 

• Intensified Verification Testing (IVT) is performed 

whenever an eligible establishment has a positive 

sample collected under the RLm sampling program 

projects, or either one of the RTEPROD sampling 

projects. 

and Salmonella samples. 

9 
 

 

 

 

 

 

Sampling by 

Program/Commodity Program Description 

Rationale for Any Changes from the FY 2021 Sampling 

Allocations 

National Residue Program 

(NRP) 

• The NRP sampling plan guides the collection of 

domestic and imported meat, poultry, and egg 

product samples. The domestic sampling plan 

includes surveillance sampling, inspector-generated, 

and special project sampling in both Federal and 

State-inspected slaughter establishments. 

• FSIS IPP perform inspector-generated sampling 

(KIS) in livestock slaughter species as per FSIS 

Directive 10,800.1. Per this directive, a positive 

sample is submitted to the FSIS laboratory for 

confirmatory testing. 

• No allocation changes for FY 2022. 

10 
 

 

 

 

 

 

 

Sampling by 

Program/Commodity Program Description 

Rationale for Any Changes from the FY 2021 Sampling 

Allocations 

Import Sampling • FSIS analyzes imported raw beef for E. coli 

O157:H7 and Salmonella. 

• FSIS analyzes imported beef manufacturing 

trimmings for non-O157 STEC, which includes the 

following six O-antigen groups: O26, O45, O103, 

O111, O121, and O145. 

• FSIS analyzes imported poultry for Salmonella and 

Campylobacter. 

• FSIS analyzes imported raw pork products for 

Salmonella. 

• FSIS analyzes imported RTE and egg products for 

Lm and Salmonella. 

• FSIS analyzes imported raw fish of the order 

Siluriformes for Salmonella. 

• FSIS analyzes imported raw meat, poultry 

products, processed products, and, including 

imported Siluriformes products for chemical 

residues. 

• No allocation changes for FY 2022. 

11 
 

 

 

 

 

 

 

 

 

Sampling by 

Program/Commodity Program Description 

Rationale for Any Changes from the FY 2021 Sampling 

Allocations 

NARMS Cecal and 

Expansion Project Sampling 

• FSIS analyzes cecal content from beef, swine, 

young chicken, turkeys, veal, sheep, goat, and lamb 

for the presence of Salmonella, Campylobacter, 

generic E. coli, and Enterococcus to monitor trends in 

antimicrobial resistance. 

• FSIS analyzes Siluriformes for the presence of 

generic E. coli and Enterococcus. 

• FSIS analyzes cattle mesenteric lymph nodes for 

the presence of Salmonella. 

• Discontinue NARMS mesenteric lymph node sampling 

in beef (300 samples). 

Other Sampling • FSIS performs verification of species claims on 

domestic and imported product. 

• FSIS performs label verification sampling for 

certain labeling claims on domestic product. 

• No allocation changes for FY 2022. 

12 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Appendices A–C outline the sampling plan grouped by product group and broken out by the 

individual sampling programs. Information for changes from previous years is provided in the 

preceding tables. Totals in the appendices’ tables have been rounded. Each table contains the 

following information: 

1. Planned number of samples to be analyzed in FY 2021; 

2. Number of samples actually analyzed in FY 2021; and 

3. Planned number of samples to be analyzed in FY 2022. 

Appendix A: Microbial Sampling Numbers by Product 

This appendix summarizes the number of samples in FSIS’ microbiological sampling program and 

presents the number of samples planned and actually analyzed in FY 2021, and the number of 

samples planned to be analyzed in FY 2022, by product type. Raw products are presented first, 

beginning with beef (Table A2), followed by pork (Table A3), fish of the order Siluriformes (Table A4) 

and poultry (Table A5). Ready-to-eat (RTE), not ready-to-eat (NRTE), and egg product sampling 

numbers are presented in Table A6. 

Table A1 is a quick reference guide of the microbiological analytes by various FSIS regulated 

products in FY 2022. For a more in-depth review, the tables in Appendix A contain the stratification 

of the different analytes by product classes. 

Table A1: Summary of Analyte tested by Product 

Microbiological Analyte 

L. E. coli Non-O157 Indicator 

Product Salmonella Campylobacter monocytogenes O157:H7 STEC Organisms 

Raw Beef √ √ √1 √2 

Raw Pork √ √2 

Raw √ Siluriformes 

Raw Poultry √ √

RTE Products √ √

Egg Products √ √

1 Only domestic raw beef manufacturing trim and imported raw beef trim. All other raw beef products are tested for 

Salmonella and E. coli O157:H7 only. 2 Dependent upon the program as not all beef and porkprojects are analyzed for indicator organisms. 

13 
Table A2: FY2021 and FY 2022 Sample Numbers for Raw Beef 

Number of Samples 

FY 2021 Sampling 

Product Class Project Code Pathogen(s) Planned Actual 

Number of Samples 

FY 2022 

Planned 

Raw ground beef MT43 E. coli O157:H7 and 11,500 

Salmonella 

10,990 11,500 

Follow-up testing to a ground MT44 and 

beef E. coli positive1 MT44T 

E. coli O157:H7 and TBD 

Salmonella 

53 TBD 

Raw ground beef 

components other than 

trim 

MT64 E. coli O157:H7 and 1,250 

Salmonella 

1,354 1,250 

Bench trim MT65 E. coli O157:H7 and 1,500 

Salmonella 

1,356 1,500 

Beef manufacturing trim MT60 E. coli O157:H7, 

Non-O157 STEC 

4,000 3,833 4,000 

and Salmonella 

Follow-up testing at 

supplier establishments 

following MT43, MT44, or 

MT65 positive1 

MT52 E. coli O157:H7, 

Non-O157 STEC 

and Salmonella 

TBD 34 TBD 

Follow-up testing to 

an MT60, MT64, 

MT65, or MT52 

positive1 

MT53 E. coli O157:H7, 

Non-O157 STEC 

and Salmonella 

TBD 549 TBD 

Raw ground beef at retail 

stores 

MT05 E. coli O157:H7and 500 

Salmonella 

520 500 

Follow-up testing to a MT05 

sample1 

MT06 E. coli O157:H7and TBD 

Salmonella 

0 TBD 

Imported raw ground beef2 MT08 E. coli O157:H7and 50 

Salmonella 

43 50 

Imported trim and other 

raw ground beef 

components2 

MT51 E. coli O157:H7, 1,200 

Non-O157 STEC and 

Salmonella 

1,962 1,200 

Exploratory beef carcasses 

pre-evisceration3 

MT_PSTHR Salmonella and 208 

Indicator Organisms 

(Aerobic Count) 

186 416 

Exploratory beef carcasses 

post-interventions3 

MT_PRECH Salmonella and 208 

Indicator Organisms 

(Aerobic Count) 

186 416 

1 Dependent on positive findings from other E. coli O157:H7 or non-O157 STEC sampling projects. 2 Lab sampling for imports depends on the number of shipments received by country and the product. 3 Allocations for these sampling projects can fluctuate depending on how many establishments are eligible and selected 

for the project. 

14 
 

 

 

 

 

 

Table A3: FY 2021 and FY 2022 Sample Numbers for Raw Pork 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Product Class Project Code Pathogen(s) Planned Actual Planned 

Comminuted Pork HC_PK_COM01 Salmonella and Indicator 8,640 6,330 8,640 

Organisms (Aerobic Count) 

Intact and Non-Intact HC_PK_CUT01 Salmonella and Indicator 2,400 2,248 2,400 

Cuts Organisms (Aerobic Count) 

Imported Pork1 IMP_PORK Salmonella 400 434 400 

1 Sampling for imports depends on the number of shipments received by country and product. 

Table A4: FY 2021 and FY 2022 Sample Numbers for Raw Siluriformes 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Product Class Project Code Analyses Planned Actual Planned 

Domestic Raw Fish EXP_FI_MIC01 Salmonella 650 583 650 

of the Order 

Siluriformes 

Imported Raw Fish IMPFISH_MI Salmonella 700 835 700 

of the Order 

Siluriformes1 

1 Sampling for imports depends on the number of shipments received by country and product. Higher than expected 

Siluriformes shipmentsreceived in FY21 resulted in increase in samples analyzed. 

Table A5: FY 2021 and FY 2022 Sample Numbers for Raw Poultry 

Number of Samples Number of Samples 

Sampling 

FY 2021 FY 2022 

Product Class Project Code Pathogen(s) Planned Actual Planned 

Young Chicken 

Carcasses 

HC_CH_CARC01 Salmonella, 

Campylobacter 

9,630 9,667 9,630 

Ground and Other HC_CH_COM01 Salmonella, 2,500 2,057 2,500 

Comminuted Campylobacter 

Chicken (not 

Mechanically 

Separated) 

15 
 

 

 

 

 

 

 

 

Number of Samples Number of Samples 

Sampling 

FY 2021 FY 2022 

Product Class Project Code Pathogen(s) Planned Actual Planned 

Exploratory - EXP_CH_MSK01 Salmonella, 150 122 150 

Mechanically 

Separated Chicken 

Campylobacter 

Chicken Parts – Legs, HC_CPT_LBW01 

Breasts, Wings 

Salmonella, 

Campylobacter 

16,300 14,093 16,300 

Chicken Parts – EXP_CPT_QH01 

Quarters, Halves 

Salmonella, 

Campylobacter 

120 81 120 

Turkey Carcasses HC_TU_CARC01 Salmonella, 1,730 1,711 1,730 

Campylobacter 

Ground and Other HC_TU_COM01 Salmonella, 1,500 1,395 1,500 

Comminuted Turkey Campylobacter 

(not Mechanically 

Separated) 

Exploratory EXP_TU_MSK01 - Salmonella, 150 111 150 

Mechanically Campylobacter 

Separated Turkey 

Imported Raw Intact IMP_POULTRY 

Chicken and Turkey1 

Salmonella, 

Campylobacter 

800 830 800 

NPIS Fowl Carcass HC_HF_CAR01 

Exploratory 

Salmonella, 

Campylobacter 240 173 240 

Follow-up Sampling F_CPT_LBW01 Salmonella, 

for Chicken Parts, F_CH_COM01 Campylobacter 

Carcasses, F_TU_COM01 

Comminuted F_CH_CARC01 TBD 1,663 TBD 

Chicken and Turkey2 F_TU_CARC01 

1 Sampling for imports depends on the number of shipments received by country and product. 2 Dependent on findings from other Salmonella and Campylobacterprojects. 

Table A6: FY 2021 and FY 2022 Sample Numbers for RTE, NRTE, and Egg Products 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Product Class Project Code Pathogen(s) Planned Actual Planned 

Both post lethality- 7,400 6,907 7,400 RTEPROD_RAND Lm, Salmonella 

exposed and non-post 

lethality-exposed RTE 

products 

Post lethality-exposed RTEPROD_RISK Lm, Salmonella 7,400 7,844 7,400 

RTE products 

16 
 

 

 

 

 

 

 

 

Number of Samples Number of Samples 

Sampling 

FY 2021 FY 2022 

Product Class Project Code Pathogen(s) Planned Actual Planned 

RLmproduct samples 

(composited 5-sample 

units) 

RLmfood contact 

surface samples 

RLmnon-foodcontact 

environmental 

samples (composited 

5-sample units) 

Intensified 

Verification Testing 

(IVT) product 

samples1 

IVT food contact 

surface samples1 

IVT non-food contact 

environmental 

samples1 

Imported intact RTE 

product3 

Egg Products 

Pasteurized imported 

liquid,frozen, or dried 

egg products 

RLMPRODC Lm 423 (2,125)2 269(1,345)2 422(2,110)2 

RLMCONT Lm 4,218 2,692 4,220 

RLMENVC Lm 423 (2,125)2 270(1,350)2 422(2,110)2 

INTPROD Lmor Salmonella TBD 380 TBD 

INTCONT Lmor Salmonella TBD 736 TBD 

INTENV Lmor Salmonella TBD 400 TBD 

IMVRTE Lm, Salmonella 3,000 3,172 3,000 

EGG_DY_MIC01 Lm, Salmonella 1,600 1,492 1,600 

EGG_LQ_MIC01 

EGGIMP Lm, Salmonella 120 102 120 

Abbreviations: RTE, ready-to-eat; NRTE, not-ready-to-eat. 

1 Dependent on positive findings from RTEPROD_RAND, RTEPROD_RISK, and RLmsampling projects. 2 The number in parenthesis represents the number of samples collected by FSIS Office of Field Operations (OFO) IPP to 

generate the composite number of samples planned. 3 Sampling for imports depends on the number of shipments received by country and product. 

17 
 

 

 

 

 

 

Appendix B: Chemical Residue Sampling Numbers by Product 

This appendix summarizes the numbers of samples in FSIS’ chemical residue sampling program for FY 

2021 and FY 2022. Chemical residues can include both drug residues and environmental 

contaminants. Table B1 presents the number of samples by production class. Tables B2 and B3 

present the number of analyses performed by method used in each production class broken out by 

domestic and import sampling. 

Table B1: FY 2021 and FY 2022 Sample Numbers for Chemical Residues 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Production Class Project Code Planned Actual Planned 

Beef Cows NRP_BC 752 787 752 

Beef Cow – State1 NRP_BC_S 48 34 48 

Bob Veal NRP_BV 400 330 400 

Bob Veal – State1 NRP_BV_S 0 0 0 

Dairy Cows NRP_DC 788 803 788 

Dairy Cows – State1 NRP_DC_S 12 15 12 

Heifers NRP_HF 340 369 340 

Heifers – State1 NRP_HF_S 60 46 60 

Steer NRP_ST 328 354 328 

Steer – State1 NRP_ST_S 72 70 72 

Market Swine NRP_MS 728 752 728 

Market Swine – State1 NRP_MS_S 72 57 72 

Sows NRP_SW 788 723 788 

Sows – State1 NRP_SW_S 12 17 12 

Young Chicken NRP_YC 394 418 394 

Young Chicken– State1 NRP_YC_S 12 11 12 

Whole Chicken NRP_WC 394 393 394 

Young Turkey NRP_YT 788 829 788 

Young Turkey – State1 NRP_YT_S 12 5 12 

Sheep NRP_SH 100 105 100 

Lambs NRP_LA 100 95 100 

Goats NRP_GO 300 309 300 

Roaster Swine NRP_RS 300 311 300 

NRP_FFV, Veal other than bob veal 150 123 150 NRP_NFFV 

Feral Swine NRP_FS 75 63 75 

Egg Products NRP_EG 250 188 250 

Siluriformes – Domestic RES_FI 650 609 650 

18 
 

 

 

 

 

Production Class 

Sampling 

Project Code 

Number of Samples 

FY 2021 

Planned Actual 

Number of Samples 

FY 2022 

Planned 

Siluriformes – Imports2 IMPFISH_CH_E and 700 

IMPFISH_CH_W 

1,728 700 

KISTM Test3 

KISTM Test – Laboratory Confirmation4 

KIS 

KIS 

NA 

NA 

154,790 

1,999 

NA 

NA 

Collector Generated Residues Various NA 147 NA 

Import Residue Various 2,000 2,032 2,000 

Abbreviations: KISTM, Kidney Inhibition Swab; NA, non-applicable. 

1 FSIS updated allocationsfor State establishments, which are part of the State meat and poultry inspection program,that 

producethe same species as those at federally inspected establishments to be based off the number of qualifying 

establishments and not a standard percentage as done previously. 

2 Sampling for imports depends on the number of shipments received by country and product. Higher than expected 

Siluriformes shipments received in FY 2021 resulted in increase in samples analyzed. 

3 These KISTMtests are performed by FSIS IPP in the field and not by the laboratories. 

4 FSIS IPP send positive KISTM tests to FSIS laboratories for confirmation. 

19 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Table B2: Planned Number of Chemical Residues Analysis by Production Class: Domestic Residue Plan 

Methods 

Beef Cows 

Numbe 

r of 

Animals 

800 

Aminoglycoside 

s 

800 

Antifunga 

l Dyes 

-

Carbado 

x 

-

Metal 

s 

100 

Multiresidue 

800 

Nitrofuran 

s 

-

Pesticide 

s 

400 

PFA 

S 

-

Sulfonamide 

s 

-

Bob Veal 400 400 - - 100 400 - 200 - -

Dairy Cows 

Heifers 

800 

400 

800 

400 

-

-

-

-

100 

100 

800 

400 

-

-

400 

200 

-

-

-

-

Steers 400 400 - - 100 400 - 200 - -

Roaster 

Swine 

300 - - 300 - - - - - -

Market 

Swine 

800 800 - - 100 800 - 400 200 -

Sows 800 800 - - 100 800 - 400 200 -

Feral Swine 75 - - - - - - 75 - -

Young 

Chickens 

400 400 - - 150 400 - - - -

Young 

Whole 

Chickens 

400 400 - - - 400 400 400 400 -

Young 

Turkeys 

Goats 

800 

300 

800 

300 

-

-

-

-

150 

-

800 

300 

400 

-

400 

-

-

-

-

-

Siluriforme 

s 

650 - 325 - 325 650 325 325 150 -

Egg 

Products 

400 - - - - 250 - 250 - -

20 
 

 

 

 

 

FormulaFed Veal 

75 75 - - - 75 - - - -

NonFormulaFed Veal 

75 75 - - - 75 - - - -

Sheep 

Lamb 

100 

100 

100 

100 

-

-

-

-

-

-

100 

100 

-

-

50 

50 

-

-

-

-

Total 8,075 6,300 325 300 1,325 7,200 1,125 3,575 950 0 

21 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Table B3: Planned Number of Chemical Residues Analysis by Production Class: Import Residue Plan 

Methods 

Beef, Raw 

Beef, Processed 

Chicken, Raw 

Aminoglycosides 

200 

-

50 

Antifungal 

Dyes 

-

-

-

Avermectins 

-

25 

-

Carbadox 

-

-

-

Metals 

50 

12 

25 

Multiresidue 

200 

-

50 

Nitrofurans 

-

-

25 

Pesticides 

100 

-

25 

PFAS 

-

-

-

Sulfonamides 

-

25 

-

Chicken, 

Processed - - - - 5 - - - - 5 

Turkey, Raw 40 - - - 10 40 25 25 - -

Turkey, 

Processed - - - - 5 - - - - 5 

Veal, Raw 

Veal, Processed 

Goat, Raw 

Goat, Processed 

Lamb, Raw 

Lamb, Processed 

Mutton, Raw 

70 

-

25 

-

20 

-

5 

-

-

-

-

-

-

-

-

5 

-

5 

-

5 

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

70 

-

25 

-

20 

-

5 

-

-

-

-

-

-

-

35 

-

25 

-

10 

-

5 

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Mutton, 

Processed - - 5 - - - - - - -

Pork, Raw 

Pork, Processed 

200 

-

-

-

-

25 

-

-

50 

12 

200 

-

-

-

100 

-

-

-

-

25 

Siluriformes, 

Raw 

- 350 - - 350 700 350 350 150 -

Egg Products - - - - - - - 40 - -

Total 610 350 70 0 519 1,310 400 715 150 60 

22 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Appendix C: National Antimicrobial Resistance Monitoring System 

(NARMS) Programs 

The National Antimicrobial Resistance Monitoring System (NARMS) is an interagency, collaborative 

partnership with State and local public health departments, the U.S. Food and Drug Administration 

(FDA), the Centers for Disease Control and Prevention (CDC), and the U.S. Department of 

Agriculture (USDA). This national public health surveillance system tracks changes in antimicrobial 

susceptibility of select foodborne enteric bacteria found in ill people (CDC), retail meats (FDA), and 

food animals (USDA FSIS). The NARMS program at USDA focuses on two sampling points: samples 

collected from intestinal (cecal) content from food animals and carcass or food commodity samples. 

While the carcass or food commodity results are derived by co-analyzing samples collected for 

existing sampling programs, the cecal sampling program involves collection of cecal content 

samples from food animals at slaughter facilities. This appendix summarizes the number of samples 

needed to execute cecal sampling through a collaborative program with the FDA. The planned 

number of samples are best estimates for the number of samples needed to execute the cecal 

program based on how many expected isolates each sample should recover. More samples may be 

necessary to fully implement the program. Table C1 summarizes how resources are attributed to 

each commodity. 

Table C1: FY 2021 and FY 2022 Sample Numbers for NARMS 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Sampling Project Description Project Code Planned Actual Planned 

NARMS-Beef Cows NARMS_BC 456 775 456 

NARMS-Veal (Bob Veal, Formula-Fed Veal, and NARMS_BV, 

Non-Formula-Fed Veal) NARMS_FFV, 480 1,032 480 

NARMS_NFFV 

NARMS-Dairy Cows NARMS_DC 980 2,104 980 

NARMS-Heifers NARMS_HF 456 903 456 

NARMS-Steers NARMS_ST 1,368 1,820 1,368 

NARMS-Mesenteric Lymph Nodes in Beef 

Cow, Dairy Cow, Heifer, and Steer 

NARMS_BC_MLN, 

NARMS_DC_MLN, 

NARMS_HF_MLN, 300 381 0 

NARMS_ST_MLN 

NARMS-Market Swine NARMS_MS 860 2,056 860 

NARMS-Sows NARMS_SW 410 1,469 410 

NARMS-Goat NARMS_GO 100 201 100 

NARMS-Lamb NARMS_LB 100 244 100 

NARMS-Sheep NARMS_SH 100 230 100 

NARMS-Young Chickens NARMS_YC 690 2,158 690 

23 
Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Sampling Project Description Project Code Planned Actual Planned 

NARMS-Young Turkeys NARMS_YT 435 1,351 435 

24 
 

 

 

 

 

 

Appendix D: Other Sampling Programs 

Table D1 summarizes the numbers of samples in FSIS’ sampling programs other than 

microbiological and chemical residue sampling programs for FY 2021 and FY 2022. 

Table D1: FY 2021 and FY 2022 Sample Numbers for FSIS Sampling Programs other 

than Microbiological and Chemical Residues 

Number of Samples Number of Samples 

FY 2021 FY 2022 

Sampling 

Sampling Project Description Project Code Planned Actual Planned 

Domestic AMR – Beef1 AMR01 150 79 150 

Import AMR – Beef1 IMPAMRBEEF 10 0 10 

Follow-up AMR01 – Beef1,2 FAMR01 NA 8 NA 

Foodborne Illness and Outbreak Sampling3,4 Various 7,000 630 7,000 

Label Verification for Nutrient Content – Raw Ground Beef EXP_LV_NUTR 200 61 200 

Label Verification– Allergens5 EXP_LV_SOY 200 4 200 

Label Verification– Antibiotic Free5 EXP_LV_ABX 400 251 400 

Label Verification– Hormone Free5 EXP_LV_HORM 200 6 200 

Species Identification– Collector Generated SPECID NA 0 NA 

Import Species Identification IMPSPECIESID 250 216 250 

Food Chemistry – Collector Generated5 FOODCHEM NA 1 NA 

Compliance Testing3,6 COMPLIAN NA 153 NA 

Pathology – Collector Generated3,7 Various NA 3,726 NA 

Import – Abnormal Container IMPABNCONT and NA 2 NA 

ABNCONT 

Abbreviation: AMR, advanced meat recovery. 1 FSIS collects and analyzes samplesin regulated establishments to verify that industry is preventing beef spinal cord material 

from entering the food supply and being misrepresented as meat. If an AMR sample is positive, additional samples are 

assigned to the establishment in PHIS through the FAMR01 sampling. 2 Dependent on positive findings from the AMR01 sampling project. 

3 Samples for these projects are not planned in advance, but rather an inspector can collect a sample on the basis of their 

findings or other circumstances. The planned samples for the Foodborne Illness and Outbreak Sampling are a baseline of 

2,000 samples plusa calculated projected number of samples that includesthe follow-up sampling. Since follow-up sampling 

is notated as TBD throughout the appendices, this notates the allocations set aside for all follow-up sampling and outbreak 

events. Actual valuesfor follow-up sampling are located within their respective product class tables. 4 FSIS collects and analyzes food samples potentially related to foodbornedisease outbreaks. Analysesare conducted to 

identify and further characterize organisms in outbreak samples. 5 FSIS performs foodand residuechemistry analysesto identify mislabeling, economic fraud, and adulteration of meat, 

poultry, and egg products. 6 FSIS investigators collect compliance samples at in-commerce businesses on a “for-cause” basis in response to complaints, 

allegations, and observations during routine or for-cause surveillance activities. 7 FSIS carries out diagnostic and consultative pathology services to identify diseases, parasites, and related conditions in 

response to the needs of field operations. 

25 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Appendix E: Terms, Definitions, and References 

Terms and Definitions 

Analyses: A target detection methodology is applied to a sample based on the sampling project. 

Analytes: The target of detection in the analysis, whether it is for microbiological pathogens, 

chemical residues, pathology diagnoses, or other various analyses. 

Analyzed: A sample was processed by the laboratory. 

Beef Manufacturing Trimmings: Beef parts of any size, including primal cuts, subprimal cuts, and 

smaller pieces of trimmings from subprimal cuts, that the producing slaughter establishment 

intends for raw, non-intact use. 

Bench Trim: Beef parts of any size, including primal cuts, subprimal cuts, and smaller pieces of 

trimmings from subprimal cuts, derived from animals slaughtered at another establishment 

intended for raw, non-intact use (i.e., not slaughtered onsite). 

Comminuted: Product that has been ground, mechanically separated, or mechanically or handdeboned and further chopped, flaked, minced, or otherwise processed to reduce particle 

size. 

Distributed: FSIS sampling task scheduling algorithm results in a sampling task to appear in PHIS. 

The algorithm may set to over distribute samples to compensate for predicted under 

performance in a particular sampling project. This excess distribution is often referred to as 

“over scheduling.” 

Follow-up sampling: Sampling that is a result of failed performance standards or incomplete 

moving windows or positive results. 

Moving Window: The results from FSIS sampling over 52 consecutive Sunday-to-Saturday weeks. 

For more information on moving windows, please see 83 FR 56046. 

Performed: A sample was collected and submitted to the laboratory. 

Planned: Quantity of samples identified by the workgroup and annual FSIS Sampling Plan. 

Routine Sample: Sample collected for sampling projects which are planned with predicted 

collection frequencies based on establishments’ regular operations. Positive routine samples, 

or other unpredicted events, may trigger additional sample collections whose samples would 

not be considered “routine.” 

Sampling Plan: A comprehensive annual Agency issuance which identifies the planned sampling 

programs, including statistical and policy basis, for a fiscal year. The data-driven strategic 

planning effort for microbiological and chemical residue sampling activities are aligned with 

the Agency’s Strategic and Annual Plan priorities. 

Sample Scheduling Frequency: The sampling frequency targeting the number of samples 

collected on an annual basis instead of focusing on specific collection rates. To collect 

samples from infrequent producers and optimize the total number of samples collected and 

analyzed, FSIS adjusts the number of samples being scheduled based on the average number 

of samples collected throughout the sampling year. 

Scheduled: A sample is specifically designated a collection date by the FSIS user in PHIS. An FSIS 

user may not be able to schedule all the samples distributed to a particular establishment in 

PHIS due to factors such as eligible project availability and other inspection activities. 

Test: See Analyses definition. 

26 
 

 

 

 

 

 

 

 

 

 

 

 

Windows: An established timeframe FSIS uses to calculate categorization. For example, poultry 

performance standards use the results from the past 52 weeks to determine an 

establishment’s category status. 

References 

Links to Agency Planning Documents 

FY 2017-2021 FSIS Strategic Plan: Food Safety and Inspection Service Strategic Plan 2017-2021 

(usda.gov) 

Past Annual Plans: Strategic Planning | Food Safety and Inspection Service (usda.gov) 

Links to Agency Sampling Plans and Programs 

Past Annual Sampling Plans: Sampling Program | Food Safety and Inspection Service (usda.gov) – 

under the Annual Sampling Reports menu 

Food Safety and Inspection Service Microbiological and Residue Sampling Programs: Report on the 

Food Safety and Inspection Service's Microbiological and Residue Sampling Programs (usda.gov) 

Links to Agency Sampling Summary Reports 

Past Annual Sampling Summary Reports: Sampling Program | Food Safety and Inspection Service 

(usda.gov) – under the Sampling Summary Reports menu 

Links to Posted Sampling Datasets 

FSIS Data Collection and Reports webpage: Sampling Results for FSIS Regulated Products | Food 

Safety and Inspection Service (usda.gov) 

Links to Agency Directives 

FSIS Directive 10,400.1: Sample Collection from Cattle Under the Bovine Spongiform 

Encephalopathy (BSE) Ongoing Surveillance Program - Revision 1 | Food Safety and Inspection 

Service (usda.gov) 

Links to NARMS information 

CDC NARMS website: www.cdc.gov/narms/reports/ 

FDA NARMS website: 

www.fda.gov/AnimalVeterinary/SafetyHealth/AntimicrobialResistance/NationalAntimicrobialResis 

tanceMonitoringSystem/default.htm 

27 
USDA NARMS website: 

National Antimicrobial Resistance Monitoring System (NARMS) | Food Safety and Inspection 

Service (usda.gov) 

FSIS Quarterly Antimicrobial Resistance (AMR) Tables: 

Microbiology | Food Safety and Inspection Service (usda.gov) 

28