# Patent

## Title
Flavin-binding glucose dehydrogenase having improved substrate specificity

## Abstract

A flavin-binding glucose dehydrogenase having high substrate specificity for D-glucose and decreased reactivity to D-xylose and/or maltose. More specifically, a flavin-binding glucose dehydrogenase having one or more amino acid substitutions at a position corresponding to position 78, position 79, position 81, position 121, position 122, position 123, position 569 and position 612 of Mucor-derived flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase enables D-glucose to be measured accurately without being susceptible to the effects of the presence of D-xylose and/or maltose, even under conditions of mounting a large amount of an enzyme such as in glucose sensors.

## Background
CROSS-REFERENCE TO RELATED APPLICATION
This application is a United States national phase application of International Application PCT/JP2012/078283 filed on Nov. 1, 2012.
TECHNICAL FIELD
The present invention relates to a flavin-binding glucose dehydrogenase having improved substrate specificity, a gene and a recombinant DNA thereof, and a method for producing flavin-binding glucose dehydrogenase having improved substrate specificity.
BACKGROUND ART
Blood glucose concentration (blood sugar level) is an important marker for diabetes. Devices for self-monitoring of blood glucose (SMBG) using electrochemical biosensors are widely used by diabetes patients as a device for monitoring their own blood sugar levels. The biosensors used in SMBG devices have conventionally used an enzyme such as glucose oxidase (GOD) that uses glucose as a substrate. However, since GOD has the characteristic of using oxygen as an electron acceptor, SMBG devices using GOD have the potential for preventing the obtaining of accurate measured values due to dissolved oxygen in the measurement sample having an effect on measured values.
On the other hand, various types of glucose dehydrogenases (GDH) are known as enzymes that also use glucose as a substrate but do not use oxygen as an electron acceptor. More specifically, a type of GDH that uses nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as coenzyme (NAD(P)-GDH) and a type of GDH that uses pyrroloquinoline quinone (PQQ) as coenzyme (PQQ-GDH) have been discovered, and these enzymes are used in the biosensors of SMBG devices. However, NAD(P)-GDH lacks enzyme stability while also having the problem of requiring addition of a coenzyme, while PQQ-GDH has low substrate specificity, causing it to act on sugar compounds other than glucose such as maltose, D-galactose or D-xylose, thereby allowing sugar compounds in measurement samples other than glucose to have an effect on measured values, and resulting in the problem of being unable to obtain accurate measured values.
Recently, PQQ-GDH has been reported to act on maltose contained in transfusion solutions when SMBG devices using PQQ-GDH as a biosensor are used by diabetes patients to measure blood sugar levels, causing measured values to be higher than actual blood sugar levels, and occurrences of disorders such as hypoglycemia caused by treatment based on those values have been reported. In addition, similar occurrences have been determined to also be possible in patients undergoing galactose tolerance tests and xylose absorption tests (see, for example, Non-Patent Document 1). In response to this, when the Pharmaceutical and Food Safety Bureau of the Ministry of Health, Labour and Welfare conducted a cross-reactivity study for the purpose of investigating effects on measured blood sugar levels in the case of having added various sugars to a glucose solution, in the case of adding maltose at 600 mg/dL, D-galactose at 300 mg/dL or D-xylose at 200 mg/dL, measured values obtained with a blood glucose monitoring kit using the PQQ-GDH method were indicated to be nearly 2.5 to 3 times higher than actual glucose concentration. Namely, measured values were determined to be made inaccurate by maltose, D-galactose and D-xylose present in measurement samples, thus resulting in a fervent desire to develop a GDH having high substrate specificity enabling specific measurement of glucose without being affected by sugar compounds causing measurement error in this manner.
With the foregoing in view, attention came to be focused on types of GDH that use coenzymes other than those previously described. For example, GDH derived from Aspergillus oryzae is reported in Non-Patent Documents 2 to 5, while flavin-binding glucose dehydrogenase that uses flavin adenine dinucleotide (FAD) as a coenzyme (to be referred to as FAD-GDH) is disclosed in Patent Documents 1 to 3.
However, although the aforementioned enzymes demonstrate the property of having low reactivity with respect to one or more types of sugar compounds that are not D-glucose, they do not have the property of having sufficiently low reactivity with respect to any of maltose, D-galactose and D-xylose. In contrast, the applicant found that flavin-binding GDHs isolated from the genus Mucor have the superior property of demonstrating sufficiently low reactivity with respect to maltose, D-galactose and D-xylose (see, for example, Patent Document 4). In addition, the use of this GDH was confirmed to enable accurate measurement of D-glucose concentration without being affected by maltose, D-galactose or D-xylose even under conditions in which these sugar compounds are present within a certain concentration range (see, for example, Patent Document 4). This superior substrate specificity is a major characteristic indicating the superiority of these Mucor-derived FAD-GDHs in terms of practical use.
On the other hand, continuing efforts are being made to shorten measurement time by further improving measurement sensitivity, further reduce the scale of measurement systems, and reduce the required size of the measurement sample in order to further improve the convenience of self-monitoring of blood glucose. For example, increasing the amount of glucose measurement enzyme mounted on a glucose sensor has been presumed as a means for improving measurement sensitivity. However, under the predicted conditions of such a usage method in which a large amount of enzyme is present, even in the case of using the previously described Mucor-derived FAD-GDH, reactivity to maltose and D-xylose present at a certain concentration or higher has been confirmed, albeit at a very low level, thus demonstrating that there continues to be room for improvement with respect to efforts to lower reactivity with respect to sugar compounds other than D-glucose.
A method has been disclosed for obtaining modified FAD-GDH in which reactivity to D-xylose has been lowered by introducing an amino acid substitution into Aspergillus-derived FAD-GDH in an attempt to modify existing FAD-GDH for the purpose of improving the substrate specificity of FAD-GDH (see, for example, Patent Documents 5 and 6). However, since Aspergillus-derived FAD-GDH has considerably higher reactivity to D-xylose in comparison with naturally-occurring Mucor-derived FAD-GDH, substrate specificity is still considered to be inadequate even with the previously disclosed modified Aspergillus species-derived FAD-GDH.
PRIOR ART DOCUMENTS
Patent Documents
Patent Document 1: Japanese Unexamined Patent Publication No. 2007-289148
Patent Document 2: Japanese Patent No. 4494978
Patent Document 3: International Publication No. WO 07/139013
Patent Document 4: Japanese Patent No. 4648993
Patent Document 5: Japanese Unexamined Patent Publication No. 2008-237210
Patent Document 6: International Publication No. WO 09/084616
Non-Patent Documents
Non-Patent Document 1: Pharmaceuticals and Medical Devices Safety Information No. 206, October 2004, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare.
Non-Patent Document 2: Studies on the glucose dehydrogenase of Aspergillus oryzae: I. Induction of its synthesis by p-benzoquinone and hydroquinone, T. C. Bak and R. Sato, Biochim. Biophys. Acta, 139, 265-276 (1967).
Non-Patent Document 3: Studies on the glucose dehydrogenase of Aspergillus oryzae: II. Purification and physical and chemical properties, T. C. Bak, Biochim. Biophys. Acta, 139, 277-293 (1967).
Non-Patent Document 4: Studies on the glucose dehydrogenase of Aspergillus oryzae: III. General enzymatic properties, T. C. Bak, Biochim. Biophys. Acta, 146, 317-327 (1967).
Non-Patent Document 5: Studies on the glucose dehydrogenase of Aspergillus oryzae: IV. Histidyl residue as an active site, T. C. Bak and R. Sato, Biochim. Biophys. Acta, 146, 328-335 (1967).
DISCLOSURE OF THE INVENTION
Problems to be Solved by the Invention
An object of the present invention is to provide a novel FAD-GDH that has superior specificity for D-glucose, is unlikely to act on sugar compounds other than D-glucose such as D-xylose or maltose, and is unlikely to be affected by these sugar compounds other than D-glucose when they are present when used to measure D-glucose.
Means for Solving the Problems
As a result of conducting extensive studies to solve the aforementioned problems, the inventors of the present invention found that modified FAD-GDHs, obtained by substituting specific amino acids residue in Mucor-derived FAD-GDH, demonstrate superior specificity for D-glucose, is unlikely to act on D-xylose or maltose, and is unlikely to be affected by these sugar compounds other than D-glucose when they are present, thereby leading to completion of the present invention.
Namely, the present invention is as described below.
(1) A flavin-binding glucose dehydrogenase having one or more amino acid substitutions at a position corresponding to an amino acid selected from the group consisting of the following (a) to (i) in the amino acid sequence indicated in SEQ ID NO: 1, an amino acid sequence having sequence identity with that amino acid sequence of 90% or more, or an amino acid sequence in which one or several amino acids have been deleted, substituted or added in that amino acid sequence:
(a) position corresponding to methionine at position 78 in the amino acid sequence described in SEQ ID NO: 1,
(b) position corresponding to tyrosine at position 79 in the amino acid sequence described in SEQ ID NO: 1,
(c) position corresponding to glutamine at position 81 in the amino acid sequence described in SEQ ID NO: 1,
(d) position corresponding to leucine at position 121 in the amino acid sequence described in SEQ ID NO: 1,
(e) position corresponding to valine at position 122 in the amino acid sequence described in SEQ ID NO: 1,
(f) position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1,
(g) position corresponding to glutamic acid at position 465 in the amino acid sequence described in SEQ ID NO: 1,
(h) position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1, and
(i) position corresponding to serine at position 612 in the amino acid sequence described in SEQ ID NO: 1; wherein,
in comparison with prior to carrying out the aforementioned substitution, the ratio of the reactivity to D-xylose to the reactivity to D-glucose (Xyl/Glc (%)) and/or the ratio of the reactivity to maltose to the reactivity to D-glucose (Mal/Glc (%)) is decreased.
(2) The flavin-binding glucose dehydrogenase described in (1) above, having one or more amino acid substitutions selected from the group consisting of the following (j) to (r):
(j) the amino acid at the position corresponding to methionine at position 78 in the amino acid sequence described in SEQ ID NO: 1 is any of glutamic acid, glutamine, cysteine or asparagine,
(k) the amino acid at the position corresponding to tyrosine at position 79 in the amino acid sequence described in SEQ ID NO: 1 is any of phenylalanine or asparagine,
(l) the amino acid at the position corresponding to glutamine at position 81 in the amino acid sequence described in SEQ ID NO: 1 is any of leucine, phenylalanine or asparagine,
(m) the amino acid at the position corresponding to leucine at position 121 in the amino acid sequence described in SEQ ID NO: 1 is any of cysteine or methionine,
(n) the amino acid at the position corresponding to valine at position 122 in the amino acid sequence described in SEQ ID NO: 1 is any of threonine, alanine or cysteine,
(o) the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is any of cysteine, phenylalanine, histidine, valine or serine,
(p) the amino acid at the position corresponding to glutamic acid at position 465 in the amino acid sequence described in SEQ ID NO: 1 is any of arginine, aspartic acid or isoleucine,
(q) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is any of phenylalanine or tyrosine, and
(r) the amino acid at the position corresponding to serine at position 612 in the amino acid sequence described in SEQ ID NO: 1 is any of cysteine or threonine.
(3) The flavin-binding glucose dehydrogenase described in (1) above, wherein the amino acid at the position corresponding to the tryptophan residue at position 569 in the amino acid sequence described in SEQ ID NO: 1 is substituted with tyrosine.
(4) The flavin-binding glucose dehydrogenase described in (1) above, wherein the amino acid at the position corresponding to the tryptophan residue at position 123 in the amino acid sequence described in SEQ ID NO: 1 is substituted with phenylalanine or valine.
(5) The flavin-binding glucose dehydrogenase described in (1) above, having the amino acid substitutions of the following (w) to (ai):
(w) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to methionine at position 78 is any of glutamic acid or asparagine,
(x) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to leucine at position 121 is methionine,
(y) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to valine at position 122 is cysteine,
(z) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to tryptophan at position 123 is any of phenylalanine or valine,
(aa) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to serine at position 612 is any of cysteine or threonine,
(ab) the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, the amino acid at the position corresponding to methionine at position 78 is asparagine, and the amino acid at the position corresponding to serine at position 612 is cysteine,
(ac) the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is phenylalanine, and the amino acid at the position corresponding to leucine at position 121 is methionine,
(ad) the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is phenylalanine, and the amino acid at the position corresponding to serine at position 612 is threonine,
(ae) the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is valine, and the amino acid at the position corresponding to leucine at position 121 is methionine,
(af) the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine,
(ag) the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is cysteine,
(ah) the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is threonine, and
(ai) the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to glutamic acid at position 465 is aspartic acid, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine.
(6) The flavin-binding glucose dehydrogenase described in any of (1) to (5) above, wherein the ratio of reactivity to D-xylose to reactivity to D-glucose (Xyl/Glc (%)) and/or the ratio of reactivity to maltose to reactivity to D-glucose (Mal/Glc (%)) is decreased by 20% or more in comparison with that prior to introduction of the aforementioned substitutions.
(7) A flavin-binding glucose dehydrogenase gene encoding the flavin-binding glucose dehydrogenase described in any of (1) to (6) above.
(8) A recombinant vector containing the flavin-binding glucose dehydrogenase gene described in (7) above.
(9) A host cell containing the recombinant vector described in (8) above.
(10) A method for producing flavin-binding glucose dehydrogenase, comprising the following steps:
(aj) a step for culturing the host cells described in (9) above,
(ak) a step for expressing flavin-binding glucose dehydrogenase gene contained in the aforementioned host cells, and
(al) a step for isolating flavin-binding glucose dehydrogenase from the aforementioned culture.
(11) A method for measuring glucose using the flavin-binding glucose dehydrogenase described in any of (1) to (6) above.
(12) A glucose assay kit containing the flavin-binding glucose dehydrogenase described in any of (1) to (6) above.
(13) A glucose sensor containing the flavin-binding glucose dehydrogenase described in any of (1) to (6) above.
Effects of the Invention
According to the present invention, FAD-GDH can be provided having superior substrate specificity in which reactivity to D-xylose and/or maltose is decreased.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a diagram showing the results of comparing the amino acid sequences of Mucor-derived FAD-GDH and P. amagasakiense-derived glucose oxidase aligned using a multiple alignment program.
FIG. 2 is a graph showing a plot of current response values versus substrate concentration measured by enzyme electrode assay using modified Mucor-derived GDH (W569Y).
FIG. 3 is a graph showing a plot of current response values versus substrate concentration measured by enzyme electrode assay using modified Mucor-derived GDH (W569YN122C).
FIG. 4 is a graph showing a plot of current response values versus substrate concentration measured by enzyme electrode assay using modified Mucor-derived GDH (W569Y/W123V).
FIG. 5 is a graph showing a plot of current response values versus substrate concentration measured by enzyme electrode assay using modified Mucor-derived GDH (W569Y/S612C).
FIG. 6 is a graph showing a plot of current response values versus substrate concentration measured by enzyme electrode assay using modified Mucor-derived GDH (W569Y/S612C/M78N).



BEST MODE FOR CARRYING OUT THE INVENTION
(Principle of Action of FAD-GDH of Present Invention and Method for Measuring Activity)
The FAD-GDH of the present invention catalyzes a reaction that forms glucono-δ-lactone by oxidizing a hydroxyl group of glucose in the presence of an electron acceptor in the same manner as known wild-type or mutant FAD-GDH.
Activity of the FAD-GDH of the present invention can be measured with the measuring system indicated below using, for example, phenazine methosulfate (PMS) and 2,6-dichloroindophenol (DCIP) as electron acceptors:

D-glucose+PMS (oxidized form)→D-glucono-δ-lactone+PMS (reduced form)  (Reaction 1)

PMS (reduced form)+DCIP (oxidized form)→PMS (oxidized form)+DCIP (reduced form)  (Reaction 2)

More specifically, in Reaction 1, PMS (reduced form) is first formed accompanying oxidation of D-glucose. DCIP is then reduced accompanying oxidation of PMS (reduced form) as the subsequent Reaction 2 proceeds. The degree of consumption of this DCIP (reduced form) can be detected as a change in the amount of absorbance at a wavelength of 600 nm, and enzyme activity can be determined based on this change.
More specifically, the activity of flavin-binding GDH can be measured in accordance with the following procedure. 2.05 mL of 50 mM phosphate buffer (pH 6.5), 0.6 mL of 1 M D-glucose solution and 0.15 mL of 2 mM DCIP solution are mixed followed by warming for 5 minutes at 37° C. Next, 0.1 mL of 15 mM PMS solution and 0.1 mL of enzyme sample solution are added to initiate the reaction. Absorbance is measured at the start of the reaction and over time, and the amount of the decrease in absorbance at 600 nm per minute (ΔA600) as the enzyme reaction proceeds is determined followed by calculating flavin-binding GDH activity in accordance with the following equation. At this time, 1 U of flavin-binding GDH activity is defined as the amount of enzyme that reduces 1 μmol of DCIP in 1 minute in the presence of D-glucose at a concentration of 200 mM at 37° C.




  
    
      
        
          GDH
          ⁢
          
            
          
          ⁢
          activity
          ⁢
          
            
          
          ⁢
          
            (
            
              U
              ⁢
              
                /
              
              ⁢
              ml
            
            )
          
        
        =
        
          
            
              -
              
                (
                
                  
                    Δ
                    ⁢
                    
                      
                    
                    ⁢
                    A
                    ⁢
                    
                      
                    
                    ⁢
                    600
                  
                  -
                  
                    Δ
                    ⁢
                    
                      
                    
                    ⁢
                    A
                    ⁢
                    
                      
                    
                    ⁢
                    
                      600
                      blank
                    
                  
                
                )
              
            
            ×
            3.0
            ×
            df
          
          
            16.3
            ×
            0.1
            ×
            1.0
          
        
      
    
    
      
        [
        
          Equation
          ⁢
          
            
          
          ⁢
          1
        
        ]
      
    
  




Furthermore, the value of 3.0 in the equation represents the liquid volume (mL) of the reaction reagents and enzyme sample, the value of 16.3 represents the millimolar molecular extinction coefficient (cm2/μmol) under the activity measurement conditions, the value of 0.1 represents the liquid volume (mL) of the enzyme solution, the value of 1.0 represents the cell path length (cm), Δ600blank represents the reduction in absorbance at 600 nm per minute in the case of initiating the reaction by adding 10 mM acetate buffer (pH 5.0) instead of enzyme sample solution, and df represents the dilution factor.
(Amino Acid Sequence of FAD-GDH of Present Invention)
The FAD-GDH of the present invention consists of the amino acid sequence indicated in SEQ ID NO: 1, an amino acid sequence having high sequence identity with that amino acid sequence, such as that having sequence identity of preferably 80% or more, more preferably 85% or more, even more preferably 90% or more, and most preferably 95% or more, or an amino acid sequence in which one or several amino acids in that amino acid sequence have been deleted, substituted or added, and has one or more amino acid substitutions at positions corresponding to amino acids selected from the location equivalent to position 78, the location equivalent to position 79, the location equivalent to position 81, the location equivalent to position 121, the location equivalent to position 122, the location equivalent to position 123, the location equivalent to position 465, the location equivalent to position 569 and the location equivalent to position 612 in the amino acid sequence described in SEQ ID NO: 1.
Preferably, the amino acid substitution at the aforementioned location equivalent to position 78 in the FAD-GDH of the present invention refers to a substitution in which methionine at the location equivalent to position 78 is substituted with any of cysteine, asparagine, glutamic acid or glutamine, the amino acid substitution at the aforementioned location equivalent to position 79 refers to a substitution in which tyrosine at the location equivalent to position 79 is substituted with any of phenylalanine or asparagine, the amino acid substitution at the aforementioned location equivalent to position 81 refers to a substitution in which glutamine at the location equivalent to position 81 is substituted with any of leucine, phenylalanine or asparagine, the amino acid substitution at the aforementioned location equivalent to position 121 refers to a substitution in which leucine at the location equivalent to position 121 is substituted with any of cysteine or methionine, the amino acid substitution at the aforementioned location equivalent to position 122 refers to a substitution in which valine at the location equivalent to position 122 is substituted with any of threonine, alanine or cysteine, the amino acid substitution at the aforementioned location equivalent to position 123 refers to a substitution in which tryptophan at the location equivalent to position 123 is substituted with any of cysteine, phenylalanine, histidine, valine or serine, the amino acid substitution at the aforementioned location equivalent to position 465 refers to a substitution in which glutamic acid at the location equivalent to position 465 is substituted with any of arginine, aspartic acid or isoleucine, the amino acid substitution at the aforementioned location equivalent to position 569 refers to a substitution in which tryptophan at the location equivalent to position 569 is substituted with any of phenylalanine or tyrosine, and the amino acid substitution at the aforementioned location equivalent to position 612 refers to a substitution in which serine at the location equivalent to position 612 is substituted with any of cysteine or threonine.
Among the FAD-GDH of the present invention, a multiple mutant having a plurality of combinations of the aforementioned substitutions is a more preferable example thereof. For example, double mutants having two combinations of the aforementioned substitutions, triple mutants having three combinations, and multiple mutants having multiple combinations of mutations are included in the present invention. Accumulation of such mutations makes it possible to produce an FAD-GDH in which action on D-xylose and/or maltose is further decreased.
In addition, in the production of a multiple mutant as described above, substitutions at locations other than those of the various substitutions listed above can also be combined. The locations of such substitutions have the potential to demonstrate synergistic effects by being introduced in combination with the substitution sites listed above even if prominent effects like those demonstrated by the aforementioned substitution sites are not demonstrated in the case of introducing those substitutions alone.
In addition, mutations that improve thermal stability or known mutations introduced for the purpose of demonstrating different types of effects such as improving resistance to pH or specific substances may also be arbitrarily combined in the FAD-GDH of the present invention in addition to mutations that make it difficult to act on D-xylose and maltose as previously described. Even in the case of having combined such different types of mutations, these FAD-GDHs are included in the present invention provided the effects of the present invention are able to be demonstrated.
For example, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutant in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence indicated in SEQ ID NO: 1 has been substituted with tyrosine, and another mutation is further combined with this mutation and introduced therein, and specific examples of other mutations include mutations in which the amino acid at the position corresponding to methionine at position 78 is either glutamic acid or asparagine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to leucine at position 121 is methionine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to valine at position 122 is cysteine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to tryptophan at position 123 is phenylalanine or valine.
Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, and the amino acid at the position corresponding to serine at position 612 is either cysteine or threonine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 569 in the amino acid sequence described in SEQ ID NO: 1 is tyrosine, the amino acid at the position corresponding to methionine at position 78 is asparagine, and the amino acid at the position corresponding to serine at position 612 is cysteine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is phenylalanine, and the amino acid at the position corresponding to leucine at position 121 is methionine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is phenylalanine, and the amino acid at the position corresponding to serine at position 612 is threonine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to tryptophan at position 123 in the amino acid sequence described in SEQ ID NO: 1 is valine, and the amino acid at the position corresponding to leucine at position 121 is methionine.
Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is cysteine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is threonine. Alternatively, an example of a preferable multiple mutant FAD-GDH of the present invention is a mutation in which the amino acid at the position corresponding to valine at position 232 in the amino acid sequence described in SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to glutamic acid at position 465 is aspartic acid, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine.
As was previously described hereinbelow, FAD-GDH of the present invention can be obtained by, for example, first acquiring a gene encoding an amino acid sequence that approximates the amino acid sequence of SEQ ID NO: 1 using an arbitrary method, and then introducing an amino acid substitution at any of the locations equivalent to prescribed locations in SEQ ID NO: 1.
Examples of methods used to introduce a target amino acid substitution include a method consisting of randomly introducing a mutation and a method consisting of introducing a site-specific mutation at a presumed site. Examples of the former method include error-prone PCR (Techniques, 1, 11-15 (1989)) and methods using XL1-Red competent cells (Stratagene) that are easily modified by being susceptible to the occurrence of errors in plasmid replication during cell proliferation. In addition, examples of the latter method include a method consisting of introducing a site-specific mutation with, for example, the commercially available Quick Change Site-Directed Mutagenesis Kit (Stratagene) by constructing a three-dimensional structure by crystal structure analysis of a target protein and selecting an amino acid predicted to impart a target effect based on that information. Alternatively, another example of the latter method consists of introducing a site-specific mutation by selecting an amino acid predicted to impart a target effect using the three-dimensional structure of a known protein having a high degree of homology with the target protein.
A “location corresponding to the amino acid sequence of SEQ ID NO: 1” referred to here, for example, refers to the same location in an alignment in the case of having aligned the amino acid sequence of SEQ ID NO: 1 with another amino acid sequence of FAD-GDH having sequence identity with SEQ ID NO: 1 (preferably of 80% or more, more preferably of 85% or more, even more preferably of 90% or more, and most preferably of 95% or more). Furthermore, sequence identity of an amino acid sequence can be calculated by a program such as the maximum matching or search homology program of Genetyx-Mac (Software Development) or by a program such as the maximum matching or multiple alignment program of DNASIS Pro (Hitachi Software).
In addition, an example of a method for specifying a “location corresponding to an amino acid” can be carried out by comparing amino acid sequences using a known algorithm such as the Lipman-Pearson algorithm, and imparting maximum identity to retained amino acid residues present in the amino acid sequence of FAD-GDH. By aligning the amino acid sequences of various types of FAD-GDH using this type of method, the same locations of homologous amino acid residues in each FAD-GDH sequence can be determined irrespective of insertions or deletions in the amino acid sequence. Since “same locations” specified in this manner are thought to be present at the same locations in a three-dimensional structure, they can be assumed to have similar effects with respect to substrate specificity of a target FAD-GDH.
Although various types of variations are presumed to exist in the FAD-GDH of the present invention within the range of the aforementioned sequence identity, all of these can be included in the FAD-GDH of the present invention provided the enzymological properties of these various types of FAD-GDH are similar to those of the FAD-GDH of the present invention described in the present description. FAD-GDH having such amino acid sequences is industrially useful since it has high substrate specificity and is not susceptible to the effects of the presence of a sugar compound other than D-glucose, such as D-xylose or maltose.
In addition, in the FAD-GDH of the present invention, it is important that the amino acid at the aforementioned location equivalent to position 78 be any of cysteine, asparagine, glutamic acid or glutamine, that the amino acid at the location equivalent to position 79 be any of phenylalanine or asparagine, that the amino acid at the location equivalent to position 81 be any of leucine, phenylalanine or asparagine, that the amino acid at the location equivalent to position 121 be any of cysteine or methionine, that the amino acid at the location equivalent to position 122 be any of threonine, alanine or cysteine, that the amino acid at the location equivalent to position 123 be any of cysteine, phenylalanine, histidine, valine or serine, that the amino acid at the location equivalent to position 465 be any of arginine, aspartic acid or isoleucine, that the amino acid at the location equivalent to position 569 be any of phenylalanine or tyrosine, or that the amino acid at the location equivalent to position 612 be any of cysteine or threonine, while it is not important as to whether or not they are the result of an unnatural substitution procedure. For example, in the case of introducing a desired substitution using as a starting material a protein in which amino acids at the aforementioned locations inherently differ from the desired residues of the present invention by using a known technology therein, those desired amino acid residues can be introduced by substitution. On the other hand, in the case of acquiring a desired protein by a known peptide total synthesis, or in the case of synthesizing an entire gene sequence so as to encode a protein having a desired amino acid sequence and then acquiring the desired protein on the basis thereof, or in the case of inherently having a sequence that is found in nature, the FAD-GDH of the present invention can be obtained without having to go through a step of unnatural substitution.
(Improvement of Substrate Specificity of FAD-GDH of Present Invention)
The FAD-GDH of the present invention is characterized by having high substrate specificity. More specifically, the FAD-GDH of the present invention is characterized by having extremely low reactivity with respect to maltose, D-galactose or D-xylose in the same manner as the genus Mucor-derived FAD-GDH described in Japanese Patent No. 4648993 previously discovered by the inventors of the present invention. More specifically, reactivity with respect to maltose, D-galactose or D-xylose is 2% or less in each case when based on a value of 100% for reactivity with respect to D-glucose. Since the FAD-GDH used in the present invention has high substrate specificity in this manner, it is capable of accurately measuring the amount of D-glucose even in samples from patients receiving administration of a transfusion solution containing maltose or samples from patients undergoing galactose tolerance tests or xylose tolerance tests without being affected by sugar compounds such as maltose, D-galactose or D-xylose contained in measurement samples. Moreover, since the FAD-GDH of the present invention has even higher substrate specificity for D-glucose than the genus Mucor-derived FAD-GDH described in Japanese Patent No. 4648993, it can be expected to demonstrate even greater industrial usefulness.
In addition, the FAD-GDH of the present invention preferably demonstrates extremely low measured values during measurements using sugar compounds such as maltose, D-galactose or D-xylose as substrates instead of D-glucose as previously described, while also preferably enables accurate measurement of D-glucose even under conditions in which there is contamination by sugar compounds such as maltose, D-galactose or D-xylose. More specifically, measured values in the case one or more contaminating sugar compounds selected from maltose, D-galactose or D-xylose are present are preferably 96% to 103%, while measured values in the case three types of contaminating sugar compounds consisting of maltose, D-galactose or D-xylose are simultaneously present are preferably 96% to 104%, based on a value of 100% for the reactivity with respect to D-glucose under conditions in which these contaminating sugar compounds are not present.
In the case of using FAD-GDH having such characteristics, D-glucose levels can be accurately measured even under circumstances in which maltose, D-galactose or D-xylose are present in a measurement sample.
Various enzymological properties possessed by FAD-GDH can be investigated using known techniques for specifying various properties of enzymes, such as by using methods described in the following examples. Various properties of enzymes can also be investigated to a certain degree in a liquid culture of a microorganism that produces various types of FAD-GDH or at an intermediate stage of the purification process, and more specifically, can be investigated using purified enzyme.
The modified FAD-GDH of the present invention is such that the ratio of reactivity to an equimolar concentration of D-xylose to reactivity to D-glucose (Xyl/Glc (%)) and/or the ratio of reactivity to an equimolar concentration of maltose to reactivity to D-glucose (Mal/Glc (%)) under reaction conditions based on the previously described activity measurement method is decreased in comparison with prior to introduction of an amino acid substitution, and is preferably decreased by 20% or more, more preferably by 30% or more, even more preferably by 40% or more, and most preferably by 50% or more.
In the case of the FAD-GDH of the present invention, only one of the ratio of Xyl/Glc (%) or Mal/Glc (%) may be decreased to a preferable degree in comparison with FAD-GDH prior to introduction of an amino acid substitution, or both may be decreased to a preferable degree. The reactivities are more preferably decreased with respect to both substrates.
In addition, since the FAD-GDH of the present invention is an enzyme that inherently has superior substrate specificity for D-glucose as previously described, measured values suggesting the effects of the presence of D-xylose and maltose are presumed to hardly be detected at all during measurement of reactivity carried out using D-xylose and maltose at molar concentrations roughly equal to normal fasting blood sugar levels (≦126 mg/dL (7 mM)). Therefore, when measuring reactivity to D-xylose and maltose with the FAD-GDH of the present invention, measurement is preferably carried out using a large amount of enzyme solution in which reactivity to an equimolar concentration of glucose is 2 U/ml or more, preferably 5 U/ml or more and more preferably 10 U/ml or more under conditions in which D-xylose and/or maltose are present at an excess concentration of, for example, 200 mM. As a result of measuring under such conditions, the effects of contaminants can be examined while presuming conditions similar to those when mounting a large amount of enzyme on a glucose sensor.
Furthermore, since the ratio of reactivity to an equimolar concentration of D-xylose to reactivity to D-glucose (Xyl/Glc (%)) and the ratio of reactivity to an equimolar concentration of maltose to reactivity to D-glucose (Mal/Glc (%)) vary according to the type and culturing conditions of the transformant or the measurement conditions of enzyme activity, it is necessary to compare their respective values before and after introducing an amino acid substitution under the same conditions.
(Examples of Naturally-Occurring FAD-GDH Serving as Source of FAD-GDH of Present Invention)
The FAD-GDH of the present invention can be acquired by using a known protein as a starting material and modifying that protein. In particular, the use of a starting material having numerous similarities with the enzymological properties desired in the FAD-GDH of the present invention is advantageous in terms of acquiring a desired FAD-GDH.
Examples of the aforementioned starting material include known FAD-GDH. Preferable examples of microorganisms derived from known FAD-GDH include microorganisms classified in the subphylum Mucoromycotina, preferably the class Mucoromycetes, more preferably the order Mucorales and even more preferably the family Mucoraceae. Specific examples of preferable starting materials for acquiring the FAD-GDH of the present invention include FAD-GDH derived from the genus Mucor, the genus Absidia or the genus Actinomucor. 
Specific examples of preferable microorganisms classified in the genus Mucor include Mucor prainii, Mucor javanicus, Mucor dimorphosporus and Mucor circinelloides f. circinelloides. More specifically, these include Mucor prainii NISL0103, Mucor javanicus NISL0111 and Mucor circinelloides f. circinelloides NISL0117. Specific examples of preferable microorganisms belonging to the genus Absidia include Absidia cylindrospora and Absidia hyalospora. More specifically, these include Absidia cylindrospora NISL0211 and Absidia hyalospora NISL0218. A specific example of preferable microorganism classified in the genus Actinomucor is Actinomucor elegans. More specifically, this includes Actinomucor elegans NISL9082. Furthermore, the aforementioned microbial strains are deposited with the Noda Institute for Scientific Research (NISL), and cultures can be obtained by going through the prescribed procedures.
(Acquisition of Gene Encoding FAD-GDH of Present Invention)
A genetic engineering technique is preferably used to efficiently acquire the FAD-GDH of the present invention. A commonly used gene cloning method is normally used to acquire a gene encoding the FAD-GDH of the present invention (hereinafter to be referred to as FAD-GDH gene). For example, in order to acquire the FAD-GDH of the present invention by using a known FAD-GDH as a starting material and modifying that known FAD-GDH, chromosomal DNA or mRNA can be extracted according to an ordinary method such as the method described in Current Protocols in Molecular Biology (Wiley Interscience, 1989) from known microbial cells or various other cells having the ability to produce FAD-GDH. Moreover, cDNA can also be synthesized using mRNA as a template. A chromosomal DNA or cDNA library can be prepared by using chromosomal DNA or cDNA obtained in this manner.
Next, suitable probe DNA is synthesized based on amino acid sequence information of known FAD-GDH and this is used to select FAD-GDH gene having high substrate specificity from a chromosomal DNA or cDNA library, or DNA containing a target gene fragment encoding FAD-GDH having high substrate specificity is amplified by a suitable polymerase chain reaction (PCR) method such as 5′-RACE or 3′-RACE by preparing suitable primer DNA based on the aforementioned amino acid sequence, after which these DNA fragments are linked to allow the obtaining of DNA containing the full length of the target FAD-GDH gene.
A method consisting of introducing a mutation into a gene encoding a starting material in the form of FAD-GDH, and selecting the FAD-GDH of the present invention having high substrate specificity by using the enzymological properties of the FAD-GDH expressed from various types of mutant genes as an indicator can be employed as a method for acquiring the FAD-GDH of the present invention having high substrate specificity by using a known FAD-GDH as a starting material.
Mutagenic treatment of the FAD-GDH gene used as a starting substance can be carried out by a known arbitrary method corresponding to the intended form of the mutation. Namely, a wide variety of methods can be used, including methods that allow a mutagenic chemical to contact and act on FAD-GDH gene or recombinant DNA incorporating that gene, ultraviolet irradiation methods, genetic engineering techniques and methods utilizing protein engineering techniques.
Examples of mutagenic chemicals used in the aforementioned mutagenic treatment include hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanine, nitrous acid, sulfurous acid, hydrazine, formic acid and 5-bromouracil.
The various conditions under which contact and action are allowed to occur can be determined corresponding to the type of chemical used, and there are no particular limitations thereon provided they are capable of actually inducing a desired mutation in Mucor-derived FAD-GDH gene. Normally, a desired mutation can be induced by allowing a mutagenic chemical to contact and act for 10 minutes or more, and preferably for 10 minutes to 180 minutes, at a reaction temperature of 20° C. to 80° C. and at a chemical concentration of preferably 0.5 M to 12 M. In the case of carrying out ultraviolet irradiation as well, irradiation can be carried out in accordance with ordinary methods as previously described (Chemistry Today, p. 24-30, June 1989).
A technique known as site-specific mutagenesis can typically be used as a method that utilizes protein engineering techniques. Examples thereof include the Kramer method (Nucleic Acids Res., 12, 9441 (1984); Methods Enzymol., 154, 350 (1987); Gene 37, 73 (1985)), the Eckstein method (Nucleic Acids Res., 13, 8749 (1985); Nucleic Acids Res., 13, 8765 (1985); Nucleic Acids Res., 14, 9679 (1986)), and the Kunkel method (Proc. Natl. Acad. Sci. U.S.A., 82, 488 (1985); Methods Enzymol., 154, 367 (1987)). Specific examples of methods for transforming base sequences present in DNA include methods using commercially available kits (such as the Transformer Mutagenesis Kit (Clontech), the EXOIII/Mung Bean Deletion Kit (Stratagene), and the Quick Change Site-Directed Mutagenesis Kit (Stratagene).
In addition, a commonly used technique known as the polymerase chain reaction method can also be used (Technique, 1, 11 (1989)).
Furthermore, in addition to the aforementioned gene modification methods, a desired modified FAD-GDH gene having high substrate specificity can be synthesized directly by organic synthesis methods or enzymatic synthesis methods.
In the case of determining or confirming the DNA base sequence of the FAD-GDH gene of the present invention after having been selected according to any of the arbitrary methods described above, a system such as the CEQ2000 Multi Capillary DNA Analysis System (Beckman Coulter) may be used.
(Vector and Host Cells Inserted with FAD-GDH of Present Invention)
The FAD-GDH gene of the present invention obtained in the manner described above can be incorporated into a vector such as a bacteriophage, cosmid or plasmid used in the transformation of prokaryotic or eukaryotic cells followed by transformation or transduction of host cells corresponding to each vector in accordance with ordinary methods.
An example of eukaryotic cells is yeast. Examples of microorganisms classified as yeast include Zygosaccharomyces species, Saccharomyces species, Pichia species and Candida species.
The inserted gene may also contain a marker gene for enabling selection of transformed cells. Examples of marker genes include genes such as URA3 or TRP1 that complement the nutrient requirements of the host. In addition, the inserted gene preferably contains a promoter or other control sequence capable of expressing the gene of the present invention in host cells (such as an enhancer sequence, terminator sequence or polyadenylation sequence). Specific examples of promoters include GAL1 promoter and ADH1 promoter. Although known methods such as methods using lithium acetate (Methods Mol. Cell. Biol., 5, 255-269 (1995)) or electroporation (J. Microbiol. Methods, 55 (2003), 481-484) can be preferably used to transform yeast, the method used is not limited thereto, but rather transformation may also be carried out by various arbitrary methods such as the spheroblast method or glass bead method.
In addition, other examples of eukaryotic host cells include mold cells such as Aspergillus species or Trichoderma species. The inserted gene preferably contains a promoter (such as a tef1 promoter) or other control sequence (such as a secretion signal sequence, enhancer sequence, terminator sequence or polyadenylation sequence) capable of expressing the gene of the present invention in host cells. In addition, the inserted gene may also contain a marker gene such as niaD or pyrG for enabling selection of transformed cells. Moreover, the inserted gene may also contain a homologous recombination region for insertion into an arbitrary chromosome site. A known method such as a method using polyethylene glycol and calcium chloride following the formation of protoplasts (Mol. Gen. Genet., 218, 99-104 (1989)) can be preferably used to transform filamentous fungi.
Examples of prokaryotic host cells include microorganisms belonging to the genus Escherichia, such as E. coli strain K-12, E. coli strain BL21(DE3), E. coli strain JM109, E. coli strain DH5α, E. coli strain W3110 or E. coli strain C600 (all of which are available from TakaraBio). Host cells inserted with DNA (transformants) are obtained by transforming or transducing these host cells. An example of a method used to introduce a recombinant vector into host cells consists of introducing recombinant DNA in the presence of calcium ions in the case the host cells are microorganisms belonging to the species E. coli. Moreover, electroporation may also be used. Commercially available competent cells (such as ECOS Competent Escherichia coli BL21(DE), Nippon Gene) may also be used.
(Production of FAD-GDH of Present Invention)
The FAD-GDH of the present invention may be produced by culturing host cells that produce the FAD-GDH of the present invention acquired in the manner described above, expressing flavin-binding glucose dehydrogenase gene contained in the aforementioned host cells, and then isolating the flavin-binding glucose dehydrogenase from the aforementioned culture.
Although YPD (2% Bacto Peptone, 1% Bacto Yeast Extract and 2% glucose) liquid medium widely used to culture Saccharomyces cerevisiae, for example, is thought to be able to be preferably used to culture the aforementioned eukaryotic host cells, other nutrient sources and components may be added either alone or in combination provided the addition thereof is able to improve the produced amount of the flavin-binding GDH used in the present invention.
Examples of carbon sources used in the medium include assimilable carbon compounds such as glucose, starch hydrolysates, glycerin, fructose or molasses. Examples of nitrogen sources include usable nitrogen compounds such as yeast extract, peptones, meat extracts, corn stiplica, soybean powder, malt extract, amino acids, ammonium sulfate or ammonium nitrate. Examples of inorganic substances include various types of salts such as sodium chloride, potassium chloride, magnesium sulfate, manganese chloride, ferrous chloride, monopotassium phosphate, dipotassium phosphate, sodium carbonate or calcium chloride. Vitamins or antifoaming agents and the like may also be added as necessary.
Examples of media used to culture the aforementioned prokaryotic host cells include media obtained by adding one or more types of inorganic salts such as sodium chloride, monopotassium phosphate, dipotassium phosphate, magnesium sulfate, magnesium chloride, ferric chloride, ferric sulfate or manganese sulfate to one or more types of nitrogen sources such as yeast extract, tryptone, peptone, beef extract, corn stiplica or soy bean or wheat bran percolate, followed by suitably adding a sugar source or vitamins and the like as necessary.
Although varying according to the microorganism cultured, culturing conditions can be suitably set by, for example, adjusting the starting pH of the medium to a pH of 5 to 10, setting the culturing temperature to 20° C. to 40° C., and setting the culturing time to 15 hours to 25 hours, 1 day to 2 days, or 3 days to 7 days, and culturing is carried out by deep aeration-agitation culturing, shake culturing or static culturing and the like.
An example of medium and culturing conditions in the case of culturing yeast of the genus Zygosaccharomyces consists of shake culturing at 30° C. and 200 rpm for 24 hours using a medium containing 2% Bacto Peptone, 1% Bacto Yeast Extract and 2% glucose. Culturing of Escherichia coli, for example, may be carried out by deep aeration-agitation culturing, shake culturing or static culturing at a culturing temperature of 10° C. to 42° C., preferably at a culturing temperature of about 25° C. for 4 hours to 24 hours, and more preferably at a culturing temperature of about 25° C. for 4 to 8 hours.
Following completion of culturing, an ordinary enzyme collection means can be used to collect flavin-binding GDH from within cultured microbial cells.
In the case the aforementioned enzyme is present in microbial cells, the enzyme is preferably collected from the microbial cells by separating the cells by, for example, an operation such as filtration or centrifugal separation. For example, a method consisting of crushing microbial cells using an ordinary crushing means such as an ultrasonic cell crusher, French press or Dyno-Mill, a method consisting of lysing microbial cell walls using a cell wall digesting enzyme such as lysozyme, or a method consisting of extracting enzyme from the microbial cells using a surfactant such as Triton X-100, can be used either alone or in combination.
In the case the aforementioned enzyme is present outside microbial cells, the microbial cells are separated by an operation such as filtration or centrifugal separation followed by recovering the supernatant. Next, insoluble matter is removed by filtration or centrifugal separation and the like to obtain an enzyme extract. In order to isolate the flavin-binding GDH from the resulting extract and purify as necessary, nucleic acid is removed as necessary followed by fractionating the extract by adding ammonium sulfate, alcohol or acetone and the like and collecting the precipitate to allow the obtaining of a crude enzyme of the FAD-GDH of the present invention.
The crude FAD-GDH of the present invention can be further purified by using any arbitrary known means. A sample of the purified FAD-GDH enzyme of the present invention can be obtained by, for example, gel filtration using Sephadex, Ultrogel or Bio-Gel and the like, adsorption and elution using an ion exchange resin, electrophoresis using polyacrylamide gel and the like, an adsorption and elution method using hydroxyapatite, a precipitation method such as sucrose density gradient centrifugation, affinity chromatography, a fractionation method using a molecular sieve film membrane or hollow fiber membrane and the like, or a combination thereof.
(D-Glucose Measurement Method Using FAD-GDH of Present Invention)
The present invention also discloses a glucose assay kit that contains the FAD-GDH of the present invention, and for example, D-glucose present in blood (blood sugar level) can be measured using the FAD-GDH of the present invention by using this glucose assay kit.
The glucose assay kit of the present invention contains FAD-GDH modified according to the present invention in amount at least sufficient for one assay. Typically, the glucose assay kit of the present invention contains, in addition to the modified FAD-GDH of the present invention, a buffer required for assay, a mediator, glucose standard solutions for preparing a calibration curve, and an indicator of use. The FAD-GDH modified according to the present invention can be provided in various forms, such as a freeze-dried reagent or solution in a suitable preservative solution.
In the case of a colorimetry type of glucose assay kit, D-glucose concentration can be measured according to that described below. A liquid or solid composition containing FAD-GDH, electron acceptor and at least one substance in the form of a reaction accelerator selected from the group consisting of N-(2-acetamide)-iminodiacetic acid (ADA), bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane (Bis-Tris), sodium carbonate and imidazole are made to be retained in the reaction layer of the glucose assay kit. Here, a pH buffer and coloring reagent are added as necessary. A sample containing glucose is then added thereto and allowed to react for a fixed period of time. During this time, absorbance is monitored that is equivalent to the maximum absorption wavelength of a pigment able to polymerize and form as a result of electrons being accepted by a discoloring electron acceptor or electron acceptor due to reduction. D-glucose concentration in the sample can be calculated based on the calibration curve preliminarily prepared using standard D-glucose concentration solutions from the rate of change in absorbance per unit time in the case of using a rate method, or from the change in absorbance until the time all of the D-glucose in the sample has been oxidized in the case of using an endpoint method.
D-glucose can be quantified by adding a mediator and coloring reagent able to be used in this method in the form of, for example, 2,6-dichloroindophenol (DCIP) to serve as an electron acceptor, and then monitoring the decrease in absorbance at 600 nm. In addition, D-glucose concentration can be calculated by adding phenazine methosulfate (PMS) as an electron acceptor and further adding nitrotetrazolium blue (NTB) as a coloring reagent, and determining the amount of diformazan formed by measuring absorbance at 570 nm. Furthermore, it goes without saying that the electron acceptor and coloring reagent used are not limited thereto.
(Glucose Sensor Containing FAD-GDH of Present Invention)
The present invention also relates to a glucose sensor that uses the FAD-GDH of the present invention. A carbon electrode, gold electrode or platinum electrode and the like is used as an electrode, and the enzyme of the present invention is immobilized on this electrode. Examples of methods for immobilizing on the electrode include the use of a crosslinking reagent, sealing in a polymer matrix, coating with a dialysis membrane, use of a photostabilized polymer, use of an electrically conductive polymer or use of an oxidation-reduction polymer, the enzyme of the present invention may be immobilized in a polymer or immobilized on an electrode together with an electron mediator typically represented by ferrocene or a derivative thereof, or a combination of these methods may be used. Typically, after immobilizing the modified FAD-GDH of the present invention on a carbon electrode using glutaraldehyde, the glutaraldehyde is blocked by treating with a reagent having amine groups.
Measurement of D-glucose concentration can be carried out in the manner described below. A buffer is placed in a constant temperature cell and maintained at a fixed temperature. Potassium ferricyanide or phenazine methosulfate and the like can be used for the mediator. An electrode immobilized with the modified FAD-GDH of the present invention is used for the working electrode along with using a counter electrode (such as a platinum electrode) and reference electrode (such as an Ag/AgCl electrode). After applying a fixed voltage to the carbon electrode and the current has reached a steady state, a sample containing D-glucose is added followed by measurement of the increase in current. D-glucose concentration in the sample can be calculated according to a calibration curve prepared using D-glucose solutions having standard concentrations.
As a specific example of measurement of glucose concentration, 1.5 U of the FAD-GDH of the present invention are immobilized on a glassy carbon (GC) electrode followed by measuring the current response to D-glucose concentration. 1.8 ml of potassium phosphate buffer (pH 6.0) and 0.2 ml of 1 M aqueous potassium hexacyanoferrate (III) (potassium ferricyanide) solution are added to the electrolysis cell. The GC electrode is connected to a BAS100B/W potentiostat (BAS), the solution is stirred at 37° C., and +500 mV is applied to the silver-silver chloride reference electrode. 1 M D-glucose solutions are added to these systems to a final concentration of 5, 10, 20, 30, 40 and 50 mM, and the steady-state current value is measured for each addition. The current values are then plotted versus the known glucose concentrations (5, 10, 20, 30, 40 and 50 mM) to prepare a calibration curve. As a result, D-glucose can be quantified with an enzyme-immobilized electrode using the FAD-binding glucose dehydrogenase of the present invention.
Since the FAD-GDH of the present invention has superior substrate specificity even when compared with Mucor-derived FAD-GDH of the prior art, it can be expected to demonstrated superior effects particularly in the case of applying to a glucose sensor in the manner described above. This is because, in a glucose sensor, the enzyme reaction is presumed to be carried out under special conditions in which a larger amount of enzyme is mounted on the sensor in comparison with the case of applying to a liquid reagent kit and the like, and under such conditions, the need to reduce the effects of coexisting substances is particularly high.
The following provides a more detailed explanation of the present invention through examples thereof. However, the technical scope of the present invention is not limited in any way by these examples.
EXAMPLE 1
(Evaluation of Substrate Specificity of the Genus Mucor-derived FAD-GDH in Yeast Expression System)
(1) Preparation of Yeast Transformant Strain Sc-Mp Expressing the Genus Mucor-Derived FAD-GDH
A recombinant plasmid (puc-MGD) that encodes the FAD-GDH gene of SEQ ID NO: 2 (described as MpGDH gene in Patent Document 4) was acquired in compliance with the method described in Patent Document 4. A PCR reaction was carried out in accordance with the protocol provided by using this as a template and using the synthetic nucleotides of SEQ ID NO: 3 and 4 and Prime STAR Max DNA polymerase (Takara). The PCR reaction liquid was electrophoresed in 1.0% agarose gel, and an approximately 2 kb “insert DNA fragment” was purified using Recochip (Takara).
In addition, plasmid pYES2/CT for expression in Saccharomyces cerevisiae (Invitrogen) was treated with restrictase KpnI (New England Biolabs) followed by electrophoresing the reaction liquid following restrictase treatment in 1.0% agarose gel, and purifying an approximately 6 kb “vector DNA fragment” using Recochip (Takara).
Continuing, the purified “insert DNA fragment” and “vector DNA fragment” were linked using the In-Fusion FID Cloning Kit (Clontech) in accordance with the protocol provided to produce recombinant plasmid pYE2C-Mp in order to express MpGDH under the control of GAL1 promoter. Furthermore, GAL1 promoter is a D-galactose-inducible promoter, and gene expression downstream from the promoter is induced by culturing in a medium containing D-galactose but not containing D-glucose. This pYES2C-Mp was confirmed to encode the gene sequence of SEQ ID NO: 2 with the CEQ2000 Multi-Capillary DNA Analysis System (Beckman Coulter). Subsequently, yeast transformant strain Sc-Mp that expresses the genus Mucor-derived FAD-GDH of SEQ ID NO: 1 was acquired by transforming pYE2C-Mp to strain Inv-Sc (Invitrogen) using an S. cerevisiae transformation kit (Invitrogen).
(2) Confirmation of GDH Activity in Strain Sc-Mp and Evaluation of Substrate Specificity
Yeast transformant strain Sc-Mp was cultured for 24 hours at 30° C. in 5 mL of pre-culture liquid medium (0.67% (w/v) amino acid-free yeast nitrogen base (BD), 0.192% (w/v) uracil-free yeast synthetic dropout medium additive (Sigma), 2.0% (w/v) raffinose). Subsequently, 1 mL of the pre-culture liquid was added to 4 mL of final culture liquid medium (0.67% (w/v) amino acid-free yeast nitrogen base, 0.192% (w/v) uracil-free yeast synthetic dropout medium additive, 2.5% (w/v) D-galactose, 0.75% (w/v) raffinose) followed by culturing for 16 hours at 30° C.
This culture liquid was then separated into microbial cells and culture supernatant by centrifugal separation (10,000×g, 4° C., 3 minutes), and when GDH activity was measured by the previously described enzyme activity measurement method, GDH activity in the culture supernatant was 13.1 U/mL. Next, when this activity measurement method was used to measure activity in a system in which an equimolar concentration of maltose or D-xylose was used for the substrate instead of D-glucose, the activity values were 0.114 U/mL and 0.215 U/ml, respectively. Namely, the “ratio of activity to maltose to activity to D-glucose (Mal/Glc (%))” and the “ratio of activity to D-xylose to activity to D-glucose (Xyl/Glc (%))” of Mucor-derived FAD-GDH expressed in strain Sc-Mp were determined to be 0.87% and 1.64%, respectively.
The values of (Mal/Glc (%)) and (Xyl/Glc (%)) of Mucor-derived FAD-GDH expressed in strain Sc-Mp were nearly equal to those obtained by measuring in the same manner after purifying FAD-GDH inherently produced in source microorganisms in the form of Mucor species. Namely, Mucor-derived FAD-GDH expressed in strain Sc-Mp was confirmed to already have sufficiently superior substrate specificity for D-glucose even when compared with various known types of FAD-GDH, and this enzyme was determined to be able to be said to be a preferable starting substance in an attempt to further improve substrate specificity through modification.
EXAMPLE 2
(Prediction of Amino Acids Located Near Substrate Binding Site in Mucor-Derived FAD-GDH, Production of Modified Mucor-derived FAD-GDH by Introduction of Mutations, and Verification of Effect of Improving Substrate Specificity)
(1) Prediction of Amino Acids Located Surrounding Substrate Binding Site in Mucor-Derived FAD-GDH
A protein having high homology with the amino acid sequence of a type of Mucor-derived FAD-GDH derived from Mucor species disclosed by the applicant in Japanese Patent No. 4648993 in the form of Mucor-derived FAD-GDH has not been found among various types of previously known proteins. Thus, it was not thought to be easy to predict the three-dimensional structure of Mucor-derived FAD-GDH or amino acids located near the active site based on the three-dimensional structure of known proteins predicted to be similar enzymes as in the case of the existence of known proteins having high homology.
As a result of expanding the range of the search to include regions having low identity by making an actual BLAST search of the Protein Data Bank (PDB), 26 amino acid sequences were found. More specifically, these included, starting with the sequence having the highest identity, 1GPE (identity: 32%, glucose oxidase), 1CF3 (identity: 31%, glucose oxidase), 1GAL (identity: 31%, glucose oxidase), 3QVP (identity: 31%, glucose oxidase), 3QVR (identity: 31%, glucose oxidase), 3FIM (identity: 26%, allyl alcohol oxidase) and 3Q9T (identity: 22%, formic acid oxidase). Among the 26 types of proteins found, five were glucose oxidases while the remainder consisted of functionally different proteins.
Among the proteins found in the BLAST search of the PDB, although that having the highest identity was glucose oxidase derived from Penicillium amagasakiense (P. amagasakiense) (PDB ID: 1 GPE), the identity thereof was low at only 32%. The amino acid sequence of the glucose oxidase of P. amagasakiense is shown in SEQ ID NO: 5. It would not be reasonable to consider that a person with ordinary skill in the art would predict that proteins having such a low degree of identity would have similar functions. Moreover, it would also not be reasonable to consider that a person with ordinary skill in the art would expect that an amino acid at the same position in one sequence corresponding to the position of an amino acid sequence in another sequence would be able to demonstrate the same functional effect by comparing amino acid sequences known to have such a low degree of identity. In actuality, the fact that it would be difficult to say that known proteins found to have this degree of identity are the same enzyme is as previously described.
Since it is recognized that it would not be reasonable to carry out analyses based on the three-dimensional structure of proteins having low identity as is described above, it was decided to acquire the three-dimensional structure of P. amagasakiense-derived glucose oxidase from a protein structure data bank (http://www.pdb.org/pdb/home/home/do), and infer those amino acids located near the substrate binding site of Mucor-derived FAD-GDH in the manner indicated below.
In general, the active center of an oxidation-reduction enzyme having flavin as a coenzyme is known to be located around the re-face of an isoalloxazine ring. Therefore, amino acids displaying the three-dimensional structure of glucose oxidase derived from P. amagasakiense with the three-dimensional structure analysis software PyMOL 0.99rc6 available from the web site, and located so as to surround the re-face of the isoalloxazine ring of FAD, were presumed to be amino acids located near the substrate binding site of glucose oxidase derived from P. amagasakiense. As a result, amino acid sequences having tyrosine at position 73, glycine at position 112, arginine at position 516 and histidine at position 563 in the amino acid sequence of SEQ ID NO: 5 were predicted to be candidates for amino acids having the possibility of being located near the substrate binding site in glucose oxidase derived from P. amagasakiense. 
Next, a comparison was made between the genus Mucor-derived FAD-GDH of SEQ ID NO: 1 and the amino acid sequence of glucose oxidase derived from P. amagasakiense of SEQ ID NO: 5 using the web-based multiple alignment program, ClustalW (http://www.genome.jp/tools/clustalw/). The results of comparing these amino acid sequences are shown in FIG. 1. Based on FIG. 1, tyrosine at position 73, glycine at position 112, arginine at position 516 and histidine at position 563 of glucose oxidase derived from P. amagasakiense were predicted to correspond to tyrosine at position 79, glycine at position 120, arginine at position 566 and histidine at position 613 in Mucor-derived FAD-GDH, and these amino acids were predicted to be located near the substrate binding site in Mucor-derived FAD-GDH.
The inventors of the present invention proposed a basic approach for creating the FAD-GDH of the present invention based on the aforementioned analysis. However, when considering the low degree of identity between Mucor-derived FAD-GDH and P. amagasakiense-derived glucose oxidase, as well as that the substrate binding site in P. amagasakiense-derived glucose oxidase was merely determined on the basis of an indirect inference, it cannot be considered to be likely that the actual enzyme three-dimensional structure would be as predicted above. In addition, even in the case the amino acids at several positions in Mucor-derived FAD-GDH were actually the same residues at the corresponding positions, it would normally be recognized by a person with ordinary skill in the art that it would be difficult to say that this is necessarily related to the three-dimensional characteristics of P. amagasakiense-derived glucose oxidase or an effect thereof. Thus, since the findings of the present invention exceed that which would be reasonably recognized or predicted by a person with ordinary skill in the art, even in the case of having actually made a search based on this approach, a considerable amount of trial and error would be required in order to obtain the findings of the present invention.
(2) Introduction of Site-Specific Mutation Near Predicted Substrate Binding Site in Mucor Species-Derived FAD-GDH
As a result of the aforementioned indirect inference, it was decided to introduce site-specific mutations consisting of substituting amino acids with various type of other amino acids at those locations predicted to have the possibility of being located near the substrate binding site in Mucor-derived FAD-GDH, namely tyrosine at position 79, glycine at position 120, arginine at position 566 and histidine at position 613 in SEQ ID NO: 1.
PCR reactions were carried out under the following conditions using recombinant plasmid pYE2C-Mp as template and the synthetic nucleotides of SEQ ID NO: 26 and 27 by using KOD-Plus- (Toyobo).
Namely, 5 μl of 10×KOD-Plus- buffer, 5 μl of a mixed solution of dNTPs prepared so that the concentration of each dNTP was 2 mM, 2 μl of 25 mM MgSO4, 50 ng of pYE2C-Mp serving as template, 15 pmol of each of the aforementioned synthetic oligonucleotides and 1 unit of KOD-Plus- were added followed by bringing to a final volume of 50 μl with sterile water. The prepared reaction liquid was incubated for 2 minutes at 94° C. followed by repeating 30 cycles consisting of 15 seconds at 94° C., 30 seconds at 55° C. and 8 minutes at 68° C. using a thermal cycler (Eppendorf).
A portion of the reaction liquid was electrophoresed in 1.0% agarose gel to confirm that DNA of approximately 8 kbp is specifically amplified. The resulting DNA was treated using restrictase DpnI (New England Biolabs) followed by cleaving the remaining template DNA, linking to the vector, transforming E. coli strain JM109 (Nippon Gene) and applying to LB-amp agar medium. The colonies that formed were inoculated into LB-amp liquid medium followed by shake culturing and isolating the plasmid DNA using the GenElute Plasmid Miniprep Kit (Sigma) in accordance with the protocol provided. The base sequence of DNA that encoded FAD-GDH in the plasmid was determined using the CEQ2000 Multi Capillary DNA Analysis System (Beckman Coulter), and a recombinant plasmid was acquired that encoded modified Mucor-derived FAD-GDH in which tyrosine at position 79 was substituted for alanine in the amino acid sequence described in SEQ ID NO: 2 (pYE2C-Mp-Y79A).
PCR reactions were similarly carried out by respectively using the combinations of synthetic nucleotides having the sequence ID numbers shown in Table 1, E. coli strain JM109 was transformed using vectors containing amplified DNA, and the base sequences of DNA encoding Mucor-derived FAD-GDH in the plasmid DNA retained by the formed colonies were determined to acquire recombinant plasmids consisting of pYE2C-Mp-Y79A, pYE2C-Mp-Y79V, pYE2C-Mp-Y79P, pYE2C-Mp-Y79C, pYE2C-Mp-Y79N, pYE2C-Mp-Y79Q, pYE2C-Mp-Y79S, pYE2C-Mp-Y79T, pYE2C-Mp-Y79H, pYE2C-Mp-Y79F, pYE2C-Mp-Y79W, pYE2C-Mp-Y79K, pYE2C-Mp-G120H, pYE2C-Mp-G120C, pYE2C-Mp-G120E, pYE2C-Mp-G120K, pYE2C-Mp-G120W, pYE2C-Mp-G120M, pYE2C-Mp-R566H, pYE2C-Mp-R566M, pYE2C-Mp-R566Y, pYE2C-Mp-R566Q, pYE2C-Mp-R566E, pYE2C-Mp-R566K, pYE2C-Mp-H613K, pYE2C-Mp-H613R, pYE2C-Mp-H613N and pYE2C-Mp-H613D in which tyrosine at position 79 for alanine, tyrosine at position 79 for valine, tyrosine at position 79 for proline, tyrosine at position 79 for cysteine, tyrosine at position 79 for arginine, tyrosine at position 79 for glutamine, tyrosine at position 79 for serine, tyrosine at position 79 for threonine, tyrosine at position 79 for histidine, tyrosine at position 79 for phenylalanine, tyrosine at position 79 for tryptophan, tyrosine at position 79 for lysine, glycine at position 120 for histidine, glycine at position 120 for cysteine, glycine at position 120 for glutamic acid, glycine at position 120 for lysine, glycine at position 120 for tryptophan, glycine at position 120 for methionine, arginine at position 566 for histidine, arginine at position 566 for methionine, arginine at position 566 for tyrosine, arginine at position 566 for glutamine, arginine at position 566 for glutamic acid, arginine at position 566 for lysine, histidine at position 613 for lysine, histidine at position 613 for arginine, histidine at position 613 for asparagine and histidine at position 613 for aspartic acid were respectively substituted in the amino acid sequence described in SEQ ID NO: 2.
(3) Evaluation of Substrate Specificity of Various Modified Mucor-Derived FAD-GDH Introduced with Site-Specific Mutations
Strain Inv-Sc was transformed and transformants were cultured in the same manner as previously described using recombinant plasmids pYE2C-Mp-Y79A, pYE2C-Mp-Y79V, pYE2C-Mp-Y79P, pYE2C-Mp-Y79C, pYE2C-Mp-Y79N, pYE2C-Mp-Y79Q, pYE2C-Mp-Y79S, pYE2C-Mp-Y79T, pYE2C-Mp-Y79H, pYE2C-Mp-Y79F, pYE2C-Mp-Y79W, pYE2C-Mp-Y79K, pYE2C-Mp-G120H, pYE2C-Mp-G120C, pYE2C-Mp-G120E, pYE2C-Mp-G120K, pYE2C-Mp-G120W, pYE2C-Mp-G120M, pYE2C-Mp-R566H, pYE2C-Mp-R566M, pYE2C-Mp-R566Y, pYE2C-Mp-R566Q, pYE2C-Mp-R566E, pYE2C-Mp-R566K, pYE2C-Mp-H613K, pYE2C-Mp-H613R, pYE2C-Mp-H613N and pYE2C-Mp-H613D encoding modified Mucor-derived FAD-GDH containing site-specific mutations, followed by measurement of FAD-GDH activity in the culture supernatant.
Continuing, the ratio of activity to an equimolar concentration of D-xylose to activity to D-glucose (Xyl/Glc (%)) and/or the ratio of activity to an equimolar concentration of maltose to activity to D-glucose (Mal/Glc (%)) were measured under reaction conditions based on the previously described activity measurement method using the culture supernatants of each of the aforementioned Mucor-derived FAD-GDH mutants for which GDH activity had been confirmed.
The values of Xyl/Glc (%) and Mal/Glc (%) along with the “Xyl/Glc ratio (%)” and “Mal/Glc ratio (%)”, which represent the relative substrate specificities exhibited by modified FAD-GDH following introduction of a site-specific mutation based on a value of 100% for the values of Xyl/Glc (%) and Mal/Glc (%) in Mucor-modified FAD-GDH before introducing a site-specific mutation, are shown in Table 1. In modified FAD-GDH in which the “Xyl/Glc ratio (%)” or “Mal/Glc ratio (%)” exceeds 100, the reactivity to D-xylose or maltose ends up being higher than FAD-GDH before introducing a site-specific mutation, thereby demonstrating decreased substrate specificity. Conversely, in modified FAD-GDH in which the “Xyl/Glc ratio (%)” or “Mal/Glc ratio (%)” is less than 100, reactivity to D-xylose or maltose decreases in comparison with FAD-GDH before introducing a site-specific mutation, thereby demonstrating increased substrate specificity, and the degree of that increase becomes larger the smaller the value. Furthermore, those mutants for which the measured value is indicated with a “-” indicate that GDH activity decreased considerably or that GDH activity was lost. The results shown in Table 1 were all measured under the same measurement conditions.













TABLE 1







Sequence








numbers of

Mal/Glc

Xyl/Glc



Amino acid
oligonucleotides
Mal/Glc
ratio
Xyl/Glc
ratio


Plasmid
mutation
used
(%)
(%)
(%)
(%)





















pYE2C-Mp
None
None
0.87
100
1.64
100


pYE2C-Mp-Y79A
Y79A
6, 7
0.98
113
1.37
84


pYE2C-Mp-Y79V
Y79V
8, 7
—
—
—
—


pYE2C-Mp-Y79P
Y79P
9, 7
—
—
—
—


pYE2C-Mp-Y79C
Y79C
10, 7 
—
—
—
—


pYE2C-Mp-Y79N
Y79N
11, 7 
0.82
94
1.17
71


pYE2C-Mp-Y79Q
Y79Q
12, 7 
4.24
488
2.23
136


pYE2C-Mp-Y79S
Y79S
13, 7 
0.98
113
1.38
84


pYE2C-Mp-Y79T
Y79T
14, 7 
—
—
—
—


pYE2C-Mp-Y79H
Y79H
15, 7 
—
—
—
—


pYE2C-Mp-Y79F
Y79F
16, 7 
1.18
135
1.03
63


pYE2C-Mp-Y79W
Y79W
17, 7 
2.13
245
2.79
170


pYE2C-Mp-Y79K
Y79K
18, 7 
—
—
—
—


pYE2C-Mp-G120H
G120H
19, 20
—
—
—
—


pYE2C-Mp-G120C
G120C
21, 20
—
—
—
—


pYE2C-Mp-G120E
G120E
22, 20
—
—
—
—


pYE2C-Mp-G120K
G120K
23, 20
—
—
—
—


pYE2C-Mp-G120W
G120W
24, 20
—
—
—
—


pYE2C-Mp-G120M
G120M
25, 20
—
—
—
—


pYE2C-Mp-R566H
R566H
26, 27
—
—
—
—


pYE2C-Mp-R566M
R566M
28, 27
—
—
—
—


pYE2C-Mp-R566Y
R566Y
29, 27
—
—
—
—


pYE2C-Mp-R566Q
R566Q
30, 27
—
—
—
—


pYE2C-Mp-R566E
R566E
31, 27
—
—
—
—


pYE2C-Mp-R566K
R566K
32, 27
—
—
—
—


pYE2C-Mp-H613K
H613K
33, 34
—
—
—
—


pYE2C-Mp-H613R
H613R
35, 34
—
—
—
—


pYE2C-Mp-H613N
H613N
36, 34
—
—
—
—


pYE2C-Mp-H613D
H613D
37, 34
—
—
—
—









As shown in Table 1, many of the modified proteins produced did not possess adequate FAD-GDH activity. In addition, even among those proteins that demonstrated FAD-GDH activity, there were those in which reactivity to D-xylose or maltose was high resulting in poor substrate specificity. Among these proteins, the values of Xyl/Glc (%) and Xyl/Glc ratio (%) were determined to decrease by respectively substituting tyrosine at position 79 in SEQ ID NO: 1 with alanine, asparagine, serine or phenylalanine. In proteins in which tyrosine was substituted with asparagine or phenylalanine in particular, the Xyl/Glc ratio (%) decreased by 20% or more, thereby demonstrating a remarkable degree of improvement of substrate specificity. In addition, in the case of the modified protein in which tyrosine at position 79 in SEQ ID NO: 1 was substituted with asparagine, values for both Mal/Glc (%) and Mal/Glc ratio (%) also decreased slightly, and it was determined that that not only reactivity with respect to D-xylose, but also reactivity with respect to maltose, is equal to or better than that prior to introducing a site-specific mutation.
Namely, as a result of respectively mutating amino acids at a plurality of sites indirectly inferred based on the approach described in (1) above, although modified forms demonstrating desired effects were unable to be acquired easily, during the course of searching, modified forms were able to be acquired that demonstrated preferable effects in the case of mutating the amino acid at the location of position 79 and substituting for a certain type of amino acid residue.
EXAMPLE 3
(Production of Modified Mucor-derived FAD-GDH Introduced with Site-specific Mutation Near Indirectly Predicted Substrate Binding Site)
(1) Introduction of Site-Specific Mutation Near Predicted Substrate Binding Site
As indicated in Example 2, since modified forms having desired properties were able to be acquired for a portion of the modified forms produced, site-specific mutations were also attempted to be introduced for those amino acids in proximity to each of tyrosine at position 79, glycine at position 120, arginine at position 566 and histidine at position 613 that were mutated in Example 2.
More specifically, recombinant plasmids encoding modified FAD-GDH introduced with a target site-specific mutation were prepared by using recombinant plasmid pYE2C-Mp as template in compliance with Example 2 by using various combinations of synthetic nucleotides as primers. Typical examples of the modified forms produced are shown in Table 2. More specifically, these modified forms consisted of mutants obtained by substituting glycine at position 77 for alanine, methionine at position 78 for cysteine, methionine at position 78 for aspartic acid, methionine at position 78 for asparagine, methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for leucine, glutamine at position 81 for phenylalanine, glutamine at position 81 for asparagine, leucine at position 121 for cysteine, leucine at position 121 for methionine, valine at position 122 for threonine, valine at position 122 for isoleucine, valine at position 122 for alanine, valine at position 122 for methionine, valine at position 122 for cysteine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, aspartic acid at position 568 for asparagine, aspartic acid at position 568 for glutamic acid, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for alanine, serine at position 612 for cysteine and serine at position 612 for threonine in SEQ ID NO: 1, and recombinant plasmids containing genes encoding these mutants were designated as pYE2C-Mp-G77A, pYE2C-Mp-M78C, pYE2C-Mp-M78D, pYE2C-Mp-M78N, pYE2C-Mp-M78E, pYE2C-Mp-M78Q, pYE2C-Mp-Q81L, pYE2C-Mp-Q81F, pYE2C-Mp-Q81N, pYE2C-Mp-L121C, pYE2C-Mp-L121M, pYE2C-Mp-V122T, pYE2C-Mp-V121I, pYE2C-Mp-V122A, pYE2C-Mp-V122M, pYE2C-Mp-V122C, pYE2C-Mp-W123C, pYE2C-Mp-W123F, pYE2C-Mp-W123H, pYE2C-Mp-W123V, pYE2C-Mp-W123 S, pYE2C-Mp-D568N, pYE2C-Mp-D568E, pYE2C-Mp-W569F, pYE2C-Mp-W569Y, pYE2C-Mp-S612A, pYE2C-Mp-S612C and pYE2C-Mp-S612T.
(2) Evaluation of Substrate Specificity of Various Types of Modified Mucor-Derived FAD-GDH Introduced with Site-Specific Mutations
Strain Inv-Sc was transformed in the same manner as Example 2 using the aforementioned recombinant plasmids pYE2C-Mp-G77A, pYE2C-Mp-M78C, pYE2C-Mp-M78D, pYE2C-Mp-M78N, pYE2C-Mp-M78E, pYE2C-Mp-M78Q, pYE2C-Mp-Q81L, pYE2C-Mp-Q81F, pYE2C-Mp-Q81N, pYE2C-Mp-L121C, pYE2C-Mp-L121M, pYE2C-Mp-V122T, pYE2C-Mp-V121I, pYE2C-Mp-V122A, pYE2C-Mp-V122M, pYE2C-Mp-V122C, pYE2C-Mp-W123C, pYE2C-Mp-W123F, pYE2C-Mp-W123H, pYE2C-Mp-W123V, pYE2C-Mp-W1235, pYE2C-Mp-D568N, pYE2C-Mp-D568E, pYE2C-Mp-W569F, pYE2C-Mp-W569Y, pYE2C-Mp-S612A, pYE2C-Mp-S612C and pYE2C-Mp-S612T encoding modified Mucor-derived FAD-GDH introduced with site-specific mutations, followed by culturing each of the transformants and confirming GDH activity in the culture supernatants.
Continuing, Mal/Glc (%), Mal/Glc ratio (%), Xyl/Glc (%) and Xyl/Glc ratio (%) were measured in the same manner as Example 2. The results are shown in Table 2.













TABLE 2







Sequence








numbers of

Mal/Glc

Xyl/Glc



Amino acid
oligonucleotides
Mal/Glc
ratio
Xyl/Glc
ratio


Plasmid
mutation
used
(%)
(%)
(%)
(%)





















pYE2C-Mp
None
None
0.87
100
1.64
100


pYE2C-Mp-G77A
G77A
38, 39
1.08
125
1.93
118


pYE2C-Mp-M78C
M78C
40, 41
0.92
106
0.77
47


pYE2C-Mp-M78D
M78D
42, 41
2.67
307
1.33
81


pYE2C-Mp-M78N
M78N
43, 41
1.06
122
0.71
43


pYE2C-Mp-M78E
M78E
44, 41
0.65
75
1.10
67


pYE2C-Mp-M78Q
M78Q
45, 41
0.45
52
0.90
55


pYE2C-Mp-Q81L
Q81L
46, 47
0.52
60
1.55
95


pYE2C-Mp-Q81F
Q81F
48, 47
0.54
62
1.99
121


pYE2C-Mp-Q81N
Q81N
49, 47
0.45
51
1.27
78


pYE2C-Mp-L121C
L121C
50, 51
1.35
156
1.18
72


pYE2C-Mp-L121M
L121M
52, 51
0.54
63
0.88
54


pYE2C-Mp-V122T
V122T
53, 54
0.24
27
1.94
118


pYE2C-Mp-V122I
V122I
55, 54
0.71
81
9.53
582


pYE2C-Mp-V122A
V122A
56, 54
0.42
48
2.08
127


pYE2C-Mp-V122M
V122M
57, 54
1.30
150
4.94
301


pYE2C-Mp-V122C
V122C
58, 54
0.46
53
1.64
100


pYE2C-Mp-W123C
W123C
59, 60
0.37
42
0.37
22


pYE2C-Mp-W123F
W123F
61, 60
0.39
45
0.65
40


pYE2C-Mp-W123H
W123H
62, 60
0.16
19
0.49
30


pYE2C-Mp-W123V
W123V
63, 60
0.42
48
0.77
47


pYE2C-Mp-W123S
W123S
64, 60
0.32
37
0.32
20


pYE2C-Mp-D568N
D568N
65, 66
—
—
—
—


pYE2C-Mp-D568E
D568E
67, 66
—
—
—
—


pYE2C-Mp-W569F
W569F
68, 69
0.28
32
0.55
34


pYE2C-Mp-W569Y
W569Y
70, 69
0.21
24
0.55
34


pYE2C-Mp-S612A
S612A
71, 72
1.06
122
1.80
110


pYE2C-Mp-S612C
S612C
73, 72
0.58
67
1.05
64


pYE2C-Mp-S612T
S612T
74, 72
0.56
64
1.20
73









On the basis of Table 2, Mal/Glc (%) and Mal/Glc ratio (%) were determined to decrease as a result of substituting methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for leucine, glutamine at position 81 for phenylalanine, glutamine at position 81 for asparagine, leucine at position 121 for methionine, valine at position 122 for threonine, valine at position 122 for isoleucine, valine at position 122 for alanine, valine at position 122 for cysteine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for cysteine or serine at position 612 for threonine in SEQ ID NO: 1.
Particularly in the case of substituting methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for leucine, glutamine at position 81 for phenylalanine, glutamine at position 81 for asparagine, leucine at position 121 for methionine, valine at position 122 for threonine, valine at position 122 for alanine, valine at position 122 for cysteine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for cysteine or serine at position 612 for threonine in SEQ ID NO: 1, Mal/Glc ratio (%) decreased by 20% or more, and the degree of improvement of substrate specificity was remarkable.
In addition, Xyl/Glc (%) and Xyl/Glc ratio (%) were determined to decrease as a result of substituting methionine at position 78 for cysteine, methionine at position 78 for aspartic acid, methionine at position 78 for asparagine, methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for leucine, glutamine at position 81 for asparagine, leucine at position 121 for cysteine, leucine at position 121 for methionine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for cysteine or serine at position 612 for threonine in SEQ ID NO: 1.
Particularly in the case of substituting methionine at position 78 for cysteine, methionine at position 78 for asparagine, methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for asparagine, leucine at position 121 for cysteine, leucine at position 121 for methionine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for cysteine or serine at position 612 for threonine in SEQ ID NO: 1, Xyl/Glc ratio (%) decreased by 20% or more, and the degree of improvement of substrate specificity was remarkable.
Moreover, in modified FAD-GDH in which methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, glutamine at position 81 for asparagine, leucine at position 121 for methionine, tryptophan at position 123 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for histidine, tryptophan at position 123 for valine, tryptophan at position 123 for serine, tryptophan at position 569 for phenylalanine, tryptophan at position 569 for tyrosine, serine at position 612 for cysteine or serine at position 612 for threonine was substituted, both Mal/Glc ratio (%) and Xyl/Glc ratio (%) decreased by 20% or more, decreased by 40% or more or 50% or more in more remarkable cases, and decreased by 60% or more in extremely remarkable cases, and were determined to have extremely high substrate specificity.
EXAMPLE 4
(Evaluation of Substrate Specificity of Multiple Mutants of Modified Mucor-derived FAD-GDH)
(1) Introduction of Multiple Substrate Specificity-Improving Mutations in Mucor-Derived FAD-GDH
A variety of mutants were acquired into which were introduced multiple target site-specific mutations by using recombinant plasmid pYE2C-Mp-W569Y as template and combining various types of synthetic nucleotides in compliance with the methods of Example 2 and Example 3 based on the findings obtained in Example 2 and Example 3. Typical examples of the modified forms produced are shown in Table 3. More specifically, these modified forms consisted of mutants obtained by substituting tryptophan at position 569 for tyrosine in SEQ ID NO: 1, and further substituting methionine at position 78 for cysteine, methionine at position 78 for asparagine, methionine at position 78 for glutamic acid, methionine at position 78 for glutamine, tyrosine at position 79 for phenylalanine, tyrosine at position 79 for asparagine, glutamine at position 81 for asparagine, leucine at position 121 for methionine, valine at position 122 for cysteine, tryptophan at position 123 for phenylalanine, tryptophan at position 123 for valine, serine at position 612 for cysteine and serine at position 612 for threonine in SEQ ID NO: 1, and recombinant plasmids containing genes encoding these mutants were designated as pYE2C-MpY-M78C, pYE2C-MpY-M78N, pYE2C-MpY-M78E, pYE2C-MpY-M78Q, pYE2C-MpY-Y79F, pYE2C-MpY-Y79N, pYE2C-MpY-Q81N, pYE2C-MpY-L121M, pYE2C-MpY-V122C, pYE2C-MpY-W123F, pYE2C-MpY-W123V, pYE2C-MpY-S612C and pYE2C-MpY-S612T.
In addition, mutants were acquired into which were introduced multiple target site-specific mutations by using recombinant plasmid pYE2C-MpY-S612C as template and combining the synthetic nucleotides shown in Table 3 in the same manner as previously described. Typical examples of the modified forms produced are shown in Table 3. These modified forms consisted of mutants obtained by substituting tryptophan at position 569 for tyrosine in SEQ ID NO: 1, and further substituting serine at position 612 for cysteine and substituting methionine at position 78 for asparagine in SEQ ID NO: 1, and the recombinant plasmid containing a gene encoding these mutants was designated as pYE2C-MpYC-M78N.
Moreover, mutants were acquired into which were introduced multiple target site-specific mutations by using recombinant plasmid pYE2C-Mp-W123F as template and combining the synthetic nucleotides shown in Table 3 in the same manner as previously described. Typical examples of the modified forms produced are shown in Table 3. More specifically, these modified forms consisted of mutants obtained by substituting tryptophan at position 123 for phenylalanine in SEQ ID NO: 1, and further substituting leucine at position 121 for methionine and substituting serine at position 612 for threonine, and recombinant plasmids containing genes encoding each of these mutants were designated as pYE2C-MpF-L121M and pYE2C-MpF-S612T.
Moreover, mutants were acquired into which were introduced multiple target site-specific mutations by using recombinant plasmid pYE2C-Mp-W123V as template and combining the synthetic nucleotides shown in Table 3 in the same manner as previously described. Typical examples of the modified forms produced are shown in Table 3. More specifically, these modified forms consisted of mutants obtained by substituting tryptophan at position 123 for valine in SEQ ID NO: 1, and further substituting leucine at position 121 for methionine and substituting serine at position 612 for threonine, and recombinant plasmids containing genes encoding each of these mutants were designated as pYE2C-MpV-L121M and pYE2C-MpV-S612T.
(2) Evaluation of Substrate Specificity of Various Types of Modified Mucor-Derived FAD-GDH Introduced with Multiple Site-Specific Mutations
Strain Inv-Sc was transformed in the same manner as previously described using recombinant plasmids pYE2C-MpY-M78C, pYE2C-MpY-M78N, pYE2C-MpY-M78E, pYE2C-MpY-M78Q, pYE2C-MpY-Y79F, pYE2C-MpY-Y79N, pYE2C-MpY-Q81N, pYE2C-MpY-L121M, pYE2C-MpY-V122C, pYE2C-MpY-W123F, pYE2C-MpY-W123V, pYE2C-MpY-S612C, pYE2C-MpY-S612T, pYE2C-MpYC-M78N, pYE2C-MpF-L121M, pYE2C-MpF-S612T, pYE2C-MpV-L121M and pYE2C-MpV-5612T encoding modified Mucor-derived FAD-GDH introduced with multiple site-specific mutations, followed by culturing each of the transformants and confirming GDH activity in the culture supernatants.
Continuing, (Mal/Glc) (%), Mal/Glc) ratio (%), (Xyl/Glc) (%) and (Xyl/Glc) ratio (%) were measured in the same manner as Example 2. The results are shown in Table 3.













TABLE 3







Sequence








numbers of

Mal/Glc

Xyl/Glc



Amino acid
oligonucleotides
Mal/Glc
ratio
Xyl/Glc
ratio


Plasmid
mutation
used
(%)
(%)
(%)
(%)





















pYE2C-Mp
None
None
0.756
100.0
1.43
100.0


pYE2C-Mp-W569Y
W569Y
68, 69
0.198
26.2
0.512
35.9


pYE2C-MpY-M78C
W569Y/M78C
40, 41
0.223
29.5
0.535
37.4


pYE2C-MpY-M78N
W569Y/M78N
43, 41
0.199
26.3
0.409
28.7


pYE2C-MpY-M78E
W569Y/M78E
44, 41
0.185
24.5
0.437
30.6


pYE2C-MpY-M78Q
W569Y/M78Q
45, 41
0.202
26.8
0.564
39.5


pYE2C-MpY-Y79F
W569Y/Y79F
16, 7 
0.273
36.1
0.512
35.9


pYE2C-MpY-Y79N
W569Y/Y79N
11, 7 
0.274
36.3
0.993
69.5


pYE2C-MpY-Q81N
W569Y/Q81N
49, 47
0.240
31.7
0.608
42.6


pYE2C-MpY-L121M
W569Y/L121M
52, 51
0.175
23.1
0.498
34.9


pYE2C-MpY-V122C
W569Y/V122C
58, 54
0.158
20.9
0.901
63.1


pYE2C-MpY-W123F
W569Y/W123F
61, 60
0.205
27.1
0.482
33.8


pYE2C-MpY-W123V
W569Y/W123V
63, 60
0.160
21.1
0.405
28.4


pYE2C-MpY-S612C
W569Y/S612C
73, 72
0.181
24.0
0.394
27.6


pYE2C-MpY-S612T
W569Y/S612T
74, 72
0.215
28.5
0.498
34.9


pYE2C-MpYC-M78N
W569Y/S612C/M78N
43, 41
0.070
9.3
0.327
22.9


pYE2C-Mp-W123F
W123F
61, 60
0.385
50.9
0.735
51.4


pYE2C-MpF-L121M
W123F/L121M
75, 60
0.375
49.6
0.550
38.5


pYE2C-MpF-S612T
W123F/S612T
74, 72
0.434
57.4
0.542
37.9


pYE2C-Mp-W123V
W123V
63, 60
0.424
56.1
0.696
48.7


pYE2C-MpV-L121M
W123V/L121M
76, 60
0.399
52.8
0.555
38.8


pYE2C-MpV-S612T
W123V/S612T
74, 72
0.465
61.5
0.775
54.3









On the basis of Table 3, Mal/We (%) and Mal/Glc ratio (%) were determined to decrease more in a double mutant obtained by substituting tryptophan at position 569 for tyrosine and further substituting valine at position 122 for cysteine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 569 for tyrosine in SEQ ID NO: 1.
In addition, Xyl/Glc (%) and Xyl/Glc ratio (%) were determined to decrease more in a double mutant obtained by substituting tryptophan at position 569 for tyrosine and further substituting methionine at position 78 for asparagine, tryptophan at position 123 for phenylalanine or serine at position 612 for threonine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 569 for tyrosine in SEQ ID NO: 1.
Moreover, Mal/Glc (%), Mal/Glc ratio (%), Xyl/Glc (%) and Xyl/Glc ratio (%) were each determined to decrease more in a double mutant obtained by substituting tryptophan at position 569 for tyrosine and further substituting methionine at position 78 for glutamic acid, leucine at position 121 for methionine, tryptophan at position 123 for valine or serine at position 612 for cysteine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 569 for tyrosine in SEQ ID NO: 1. Namely, several of the double mutants produced were determined to further improve substrate specificity in comparison with single mutants.
In addition, as shown in Table 3, Mal/Glc (%), Mal/Glc ratio (%), Xyl/Glc (%) and Xyl/Glc ratio (%) were each determined to decrease more in a triple mutant obtained by substituting tryptophan at position 569 for tyrosine, substituting serine at position 612 for cysteine and further substituting methionine at position 79 for asparagine in SEQ ID NO: 1 in comparison with a double mutant obtained by substituting tryptophan at position 569 for tyrosine and substituting serine at position 612 for cysteine in SEQ ID NO: 1. This multiple mutant can be said to demonstrate even more superior substrate specificity in addition to the effects of each mutation site.
In addition, as shown in Table 3, Xyl/Glc (%) and Xyl/Glc ratio (%) were determined to decrease more in a double mutant obtained by substituting tryptophan at position 123 for phenylalanine and further substituting serine at position 612 for threonine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 123 for phenylalanine.
In addition, Mal/Glc (%), Mal/Glc ratio (%), Xyl/Glc (%) and Xyl/Glc ratio (%) were each determined to decrease more in a double mutant obtained by substituting tryptophan at position 123 for phenylalanine and further substituting leucine at position 121 for methionine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 123 for phenylalanine. This multiple mutant can be said to demonstrate even more superior substrate specificity in addition to the effects of each mutation site.
Moreover, as shown in Table 3, Mal/Glc (%), Mal/Glc ratio (%), Xyl/Glc (%) and Xyl/Glc ratio (%) were each determined to decrease more in a double mutant obtained by substituting tryptophan at position 123 for valine and further substituting leucine at position 121 for methionine in SEQ ID NO: 1 in comparison with a single mutant obtained by substituting tryptophan at position 123 for valine in SEQ ID NO: 1. This multiple mutant can be said to demonstrate even more superior substrate specificity in addition to the effects of each mutation site.
EXAMPLE 5
(Evaluation of Substrate Specificity of Various Mucor-derived Modified FAD-GDH by Measuring with an Enzyme Electrode)
(1) Concentration of Crude Enzyme Liquids of Various Modified Mucor-Derived FAD-GDH
Strain Inv-Sc was transformed using each of the previously acquired recombinant plasmids pYE2C-Mp-W569Y, pYE2C-MpY-V122C, pYE2C-MpY-W123V, pYE2C-MpY-S612C and pYE2C-MpYC-M78N, and each transformant was cultured for 24 hours at 30° C. in 100 mL of pre-culture liquid medium (0.67% (w/v) amino acid-free yeast nitrogen base (BD), 0.192% (w/v) uracil-free yeast synthetic dropout medium additive (Sigma), 2.0% (w/v) raffinose). Subsequently, the entire amount of the pre-culture liquid medium was added to 1 L of final culture liquid medium (0.67% (w/v) amino acid-free yeast nitrogen base, 0.192% (w/v) uracil-free yeast synthetic dropout medium additive, 2.5% (w/v) D-galactose, 0.75% (w/v) raffinose) followed by culturing for 16 hours at 30° C.
The culture liquid was separated into microbial cells and supernatant by centrifugal separation (12,000×g, 4° C., 30 minutes) followed by recovery of the culture supernatant. Subsequently, the recovered culture supernatant was subjected to ultrafiltration treatment using an AIP-1013 ultrafiltration membrane (Asahi Chemicals), followed by concentrating using the AMICON Ultra-15 10K (Millipore) until the activity of the enzyme liquid reached 1000 U/mL or more.
(2) Evaluation of Substrate Specificity of Various Modified Mucor-Derived FAD-GDH by Measuring with an Enzyme Electrode)
Substrate specificity of the concentrated crude enzyme liquids of various modified Mucor-derived FAD-GDH acquired as described above was evaluated by measuring with an enzyme electrode. More specifically, 2 μL of a solution obtained by dissolving potassium ferricyanate having a final concentration of 364 mM, phosphate buffer (pH 6.0) having a final concentration of about 100 mM and a concentrate of each enzyme having a concentration of 1000 U/mL were placed on a DEP Chip electrode (round, equipped with carbon dam ring, Bio Device Technology) printed with a carbon working electrode and silver/silver chloride reference electrode, followed by allowing to stand undisturbed for 20 minutes at 35° C. to immobilize the enzyme on the electrode (2 U/strip). Subsequently, the DEP Chip was connected to an HSV-100 Automatic Polarization System (Hokuto Denko) using a special-purpose connector. 20 μL of D-glucose, maltose and D-xylose solutions having a prescribed concentration were respectively placed on the electrode and allowed to react by applying a voltage of 300 mV followed by measurement of the current value 20 seconds later. The results of plotting the current response values versus each of reacted substrate concentrations are shown in FIGS. 2 to 6. In addition, the reactivity to maltose based on a value of 100% for reactivity to D-glucose (Mal/Glc (%)) as well as reactivity to D-xylose based on a value of 100% for reactivity to D-glucose (Xyl/Glc (%)) at respective substrate concentrations of 20 mM are shown in Table 4.











TABLE 4







Glc
Mal/Glc
Xyl/Glc


Plasmid
Amino acid mutation
(%)
(%)
(%)



















pYE2C-Mp-W569Y
W569Y
100
0
4


pYE2C-MpY-V122C
W569Y/V122C
100
−5
19


pYE2C-MpY-W123V
W569Y/W123V
100
−5
−3


pYE2C-MpY-S612C
W569Y/S612C
100
3
0


pYE2C-MpYC-M78N
W569Y/S612C/M78N
100
−1
4









On the basis of FIGS. 2 to 6, D-glucose concentrations and current response values demonstrated a favorable correlation in each of the genus Mucor-derived modified FAD-GDHs, and on the basis thereof, each Mucor-derived modified FAD-GDH was determined to have superior glucose quantifiability.
However, in contrast to the value of Xyl/Glc (%) of the modified FAD-GDH having only the single mutation W569Y being 4%, the modified FAD-GDH having the mutation W569YN122C demonstrated an Xyl/Glc (%) value of 19%, and was determined to have remarkable reactivity to D-xylose although demonstrating hardly any reactivity to maltose.
On the other hand, as shown in Table 4, the genus Mucor-derived modified FAD-GDHs having the mutations of W569Y, W569Y/W123V, W569Y/S612C and W569Y/S612C/M78N were determined to demonstrate hardly any reactivity to maltose and D-xylose even when evaluated by measuring with an enzyme electrode.
Furthermore, in the measurement system based on absorbance of Example 4, while the value of Xyl/Glc (%) of the modified FAD-GDH having only the mutation W569Y was 0.512%, the value of Xyl/Glc (%) in the modified FAD-GDH having the mutation W569YN122C was 0.901%. Namely, even if the difference in Xyl/Glc (%) in a measurement system based on absorbance in Examples 1 to 4 is only about 0.5%, in the case of comparing with a measurement system based on an enzyme electrode measurement system, the difference in the substrate specificity thereof was determined to appear more remarkably. On the basis of this finding as well, the various mutants having improved substrate specificity discovered in the present invention are required to have lower values for Xyl/Glc (%) and/or Mal/Glc (%) in particular, and this is strongly suggested to be extremely useful in applications in glucose sensors and the like in which the effect thereof is presumed to be considerable.
EXAMPLE 6
(Prediction of Amino Acids Located Near Substrate Binding Site in Mucor-derived FAD-GDH, Production of Modified Mucor-derived FAD-GDH by Introduction of Mutations, and Verification of Effect of Improving Substrate Specificity—Part 2)
The three-dimensional structure of glucose oxidase derived from P. amagasakiense was displayed in accordance with Example 2, and aspartic acid at position 428 was predicted to be a candidate for an amino acid located so as to surround the re-face of the isoalloxazine ring of FAD. According to FIG. 1, aspartic acid at position 428 of glucose oxidase derived from P. amagasakiense was predicted to be equivalent to aspartic acid at position 471 of SEQ ID NO: 1, and this amino acid was predicted to be located near the substrate-binding site in Mucor-derived FAD-GDH. Therefore, it was decided to introduce site-specific mutations for aspartic acid at position 471 in SEQ ID NO: 1 and its surrounding amino acids that substitute these amino acids with various types of amino acids.
More specifically, recombinant plasmids encoding modified FAD-GDH introduced with target site-specific mutations were produced using recombinant plasmid pYE2C-Mp as template in compliance with Example 2 by using combinations of each of the synthetic nucleotides shown in Table 5 as primers. Typical examples of the produced modified forms are shown in Table 5. More specifically, these modified forms consisted of mutants obtained by substituting glutamic acid at position 465 for aspartic acid, glutamic acid at position 465 for glycine, glutamic acid at position 465 for isoleucine, glutamic acid at position 465 for arginine, glutamic acid at position 465 for leucine, glutamic acid at position 465 for serine, glutamic acid at position 465 for threonine, glutamic acid at position 465 for valine, glutamic acid at position 465 for tryptophan, asparagine at position 469 for aspartic acid, asparagine at position 469 for glutamine, asparagine at position 469 for glutamic acid, aspartic acid at position 471 for asparagine, aspartic acid at position 471 for glutamic acid, aspartic acid at position 471 for glutamine, glutamine at position 473 for glutamic acid, glutamine at position 473 for asparagine, glutamine at position 473 for aspartic acid, asparagine at position 474 for aspartic acid, asparagine at position 474 for glutamine, asparagine at position 474 for glutamic acid, asparagine at position 475 for aspartic acid, asparagine at position 475 for glutamine and asparagine at position 475 for glutamic acid in SEQ ID NO: 1, and recombinant plasmids containing genes encoding each of these mutants were designated pYE2C-Mp-E465D, pYE2C-Mp-E465G, pYE2C-Mp-E465I, pYE2C-Mp-E465R, pYE2C-Mp-E465L, pYE2C-Mp-E4655, pYE2C-Mp-E465T, pYE2C-Mp-E465V, pYE2C-Mp-E46W, pYE2C-Mp-N469D, pYE2C-Mp-N469Q, pYE2C-Mp-N469E, pYE2C-Mp-D471N, pYE2C-Mp-D471E, pYE2C-Mp-D471Q, pYE2C-Mp-Q473E, pYE2C-Mp-Q473N, pYE2C-Mp-Q473D, pYE2C-Mp-N474D, pYE2C-Mp-N474Q, pYE2C-Mp-N474E, pYE2C-Mp-N475D, pYE2C-Mp-N475Q and pYE2C-Mp-N475E.
Strain Inv-Sc was transformed in the same manner as Example 2 using the aforementioned recombinant plasmids encoding modified Mucor-derived FAD-GDH introduced with site-specific mutations consisting of pYE2C-Mp-E465D, pYE2C-Mp-E465G, pYE2C-Mp-E4651, pYE2C-Mp-E465R, pYE2C-Mp-E465L, pYE2C-Mp-E465S, pYE2C-Mp-E465T, pYE2C-Mp-E465V, pYE2C-Mp-E46W, pYE2C-Mp-N469D, pYE2C-Mp-N469Q, pYE2C-Mp-N469E, pYE2C-Mp-D471N, pYE2C-Mp-D471E, pYE2C-Mp-D471Q, pYE2C-Mp-Q473E, pYE2C-Mp-Q473N, pYE2C-Mp-Q473D, pYE2C-Mp-N474D, pYE2C-Mp-N474Q, pYE2C-Mp-N474E, pYE2C-Mp-N475D, pYE2C-Mp-N475Q and pYE2C-Mp-N475E, followed by culturing each transformant and confirming GDH activity in the culture supernatant.
Continuing, (Xyl/Glc) ratio (%) was measured in the same manner as Example 2. The results are shown in Table 5. Furthermore, those mutants for which the measured value is indicated with a “-” indicate that GDH activity decreased considerably or that GDH activity was lost.










TABLE 5






Amino
Sequence numbers of




acid
oligonucleotides
Xyl/Glc


Plasmid
mutation
used
ratio (%)


















pYE2C-Mp
None
None
100


pYE2C-Mp-E465D
E465D
77, 78
64


pYE2C-Mp-E465G
E465G
79, 78
101


pYE2C-Mp-E465I
E465I
80, 78
88


pYE2C-Mp-E465R
E465R
81, 78
55


pYE2C-Mp-E465L
E465L
82, 78
111


pYE2C-Mp-E465S
E465S
83, 78
109


pYE2C-Mp-E465T
E465T
84, 78
107


pYE2C-Mp-E465V
E465V
85, 78
99


pYE2C-Mp-E465W
E465W
86, 78
108


pYE2C-Mp-N469D
N469D
87, 88
95


pYE2C-Mp-N469Q
N469Q
89, 88
99


pYE2C-Mp-N469E
N469E
90, 88
96


pYE2C-Mp-D471N
D471N
91, 92
109


pYE2C-Mp-D471E
D471E
93, 92
101


pYE2C-Mp-D471Q
D471Q
94, 92
—


pYE2C-Mp-Q473E
Q473E
95, 96
92


pYE2C-Mp-Q473N
Q473N
97, 96
99


pYE2C-Mp-Q473D
Q473D
98, 96
109


pYE2C-Mp-N474D
N474D
 99, 100
94


pYE2C-Mp-N474Q
N474Q
101, 100
112


pYE2C-Mp-N474E
N474E
102, 100
98


pYE2C-Mp-N475D
N475D
103, 100
91


pYE2C-Mp-N475Q
N475Q
104, 100
98


pYE2C-Mp-N475E
N475E
105, 100
101









On the basis of Table 5, Xyl/Glc ratio (%) was determined to decrease by substituting glutamic acid at position 465 for aspartic acid, glutamic acid at position 465 for isoleucine or glutamic acid at position 465 for arginine in SEQ ID NO: 1.
In the case of substituting glutamic acid of SEQ ID NO: 1 for aspartic acid or substituting glutamic acid at position 465 for arginine in particular, Xyl/Glc ratio (%) decreased by 20% or more and the degree of improvement of substrate specificity was remarkable.
Moreover, in modified FAD-GDH obtained by substituting glutamic acid of SEQ ID NO: 1 for aspartic acid and substituting glutamic acid at position 465 for arginine, the values of the Mal/Glc ratio (%) were 79% and 72%, respectively, both the Mal/Glc ratio (%) and Xyl/Glc ratio (%) decreased by 20% or more, and substrate specificity was determined to be extremely high.
EXAMPLE 7
(Acquisition and Evaluation of Performance of FAD-GDH-expressing Yeast Introduced with Mutation for Improving Heat Resistance and Mutation for Improving Substrate Specificity)
The inventors of the present invention previously discovered that modified FAD-GDH obtained by substituting valine at position 232 for glutamic acid, threonine at position 387 for alanine and isoleucine at position 545 for threonine in SEQ ID NO: 1 have superior heat resistance. Therefore, it was decided to produce modified FAD-GDH having superior heat resistance and substrate specificity by causing various types of mutations for improving substrate specificity to accumulate in a triple mutant in which these three mutations for improving heat resistance were accumulated.
More specifically, site-specific mutations were sequentially introduced using recombinant plasmid pYE2C-Mp as template in compliance with Example 2 by using combinations of the synthetic nucleotides of SEQ ID NO: 106 and 107, SEQ ID NO: 108 and 109 and SEQ ID NO: 110 and 111 to produce recombinant plasmid YE2C-Mp-V232E/T387A/I545T encoding modified FAD-GDH obtained by substituting valine at position 232 for glutamic acid, threonine at position 387 for alanine and isoleucine at position 545 for threonine in SEQ ID NO: 1.
Next, recombinant plasmids encoding modified FAD-GDH introduced with target site-specific mutations were produced by using the recombinant plasmid pYE2C-Mp-V232E/T387A/I545T as template and using combinations of each of the synthetic nucleotides shown in Table 6 as primers. The produced modified forms are shown in Table 6. More specifically, these modified forms consisted of mutants obtained by substituting valine at position 232 for glutamic acid, threonine at position 387 for alanine and isoleucine at position 545 for threonine, and substituting leucine at position 121 for methionine, or, and tryptophan at position 569 for tyrosine, or, and serine at position 612 for cysteine or serine at position 612 for threonine, and recombinant plasmids containing genes encoding each of these mutations were designated pYE2C-Mp-V232E/T387A/I545T/L121M, pYE2C-Mp-V232E/T387A11545T/W569Y, pYE2C-Mp-V232E/T387A/I545T/S612C and pYE2C-Mp-V232E/T387A/I545T/S612T.
Moreover, recombinant plasmid pYE2C-Mp-V232E/T387A/E465D/I545T/W569Y encoding modified FAD-GDH obtained by substituting valine at position 232 for glutamic acid, threonine at position 387 for alanine, glutamic acid at position 465 for aspartic acid, isoleucine at position 545 for threonine and tryptophan at position 569 for tyrosine was produced by using recombinant plasmid pYE2C-Mp-V232E/T387A/I545T/W569Y as template and using combinations of the synthetic nucleotides of SEQ ID NO: 77 and 78 as primers.
Strain Inv-Sc was transformed in the same manner as Example 2 using the recombinant plasmids encoding modified Mucor-derived FAD-GDH introduced with site-specific mutations consisting of pYE2C-Mp-V232E/T387A/I545T/L121M, pYE2C-Mp-V232E/T387A/I545T/W569Y, pYE2C-Mp-V232E/T387A/I545T/S612C and pYE2C-Mp-V232E/T387A/I545T/S612T, pYE2C-Mp-V232E/T387A/E465D/1545T/W569Y, followed by culturing each of the transformants, confirming GHD activity in the culture supernatant, and measuring Xyl/Glc (%) and Xyl/Glc ratio (%) in the same manner as Example 2. The results are shown in Table 6.
Continuing, heat resistance of the modified FAD-GDH was evaluated using the same culture supernatant. More specifically, the evaluated FAD-GDH was diluted with enzyme diluent (10 mM acetate buffer, pH 5.0) to about 0.5 U/ml. Two aliquots of this enzyme solution (0.2 ml) were prepared, and one of the aliquots was stored at 4° C. while the other was subjected to heat treatment for 15 minutes at 60° C. Following heat treatment, the FAD-GDH activity of each sample was measured and the activity level after heat treatment for 15 minutes at 60° C. based on a value of 100 for the enzyme activity of the enzyme solution stored at 4° C. was calculated as “residual activity (%)”. This residual activity (%) was used as an indicator for evaluating heat resistance of each modified FAD-GDH. The results are shown in Table 6.











TABLE 6






Primer
Residual





sequence
activity
Xyl/Glc
Xyl/Glc


Amino acid substitution site
number
(%)
(%)
ratio (%)




















V232E/
—
—
18.2
2.0
100


T387A/
L121M
52, 51
0
2.4
121


I545T
W569Y
70, 69
55.4
0.8
37



S612C
73, 72
14.5
1.6
81



S612T
74, 72
19.4
1.5
74



E465D/W569Y
77, 78
49.6
0.4
19









As shown in Table 6, substrate specificity improved as a result of introducing W569Y, S612C or S612T into V232E/Y387A/I545T in comparison with prior to introduction. In addition, substrate specificity further improved as a result of introducing E465D into V232E/T387A/I545T/W569Y in comparison with prior to introduction. Moreover, heat resistance was determined to improve in modified FAD-GDH obtained by introducing W569Y into V232E/T387A/I545T in comparison with V232E/T387A/I545T.
Continuing, Mal/Glc ratio (%) was investigated in V232E/T387A/I545T/W569Y and V232E/T387A/E465D/1545T/W569Y. The Mal/Glc ratio (%) of V232E/T387A/I545T/W569Y was 34% and the Mal/Glc ratio (%) of V232E/T387A/E465D/I545T/W569Y was 40% based on a value of 100 for the Mal/Glc ratio (%) of V232E/T387A/I545T, and reactivity to maltose was also determined to decrease.
On the basis of these results, V232E/T387A/I545T/W569Y, V232E/T387A/I545T/S612C, V232E/T387A/I545T/S612T and V232E/T387A/E465D/1545T/W569Y were determined to have superior heat resistance and substrate specificity. In particular, V232E/T387A/I545T/W569Y and V232E/T387A/E465D/1545T/W569Y were determined to be extremely superior mutants since they further improved heat resistance in addition to decreasing reactivity to maltose and xylose by 20% or more in comparison with V232E/T387A/I545T.
As has been described above, since the FAD-GDH of the present invention demonstrates sufficiently high substrate specificity for D-glucose while also demonstrating sufficiently low reactivity to sugar compounds other than D-glucose such as D-xylose and maltose, D-glucose can be measured accurately even in cases of measuring D-glucose in a sample under conditions of containing large amounts of sugar compounds other than D-glucose or under conditions of a high enzyme concentration, and is expected to enable measurement with higher precision and higher sensitivity in comparison with the case of using conventional FAD-GDH in applications such as glucose sensors.

## Claims

1. A flavin-binding glucose dehydrogenase having the following amino acid substitutions in SEQ ID NO: 1 or amino acid sequence having a 90% or more sequence identity with the amino acid sequence of SEQ ID NO: 1:
the amino acid at the position corresponding to methionine at position 78 is selected from the group consisting of glutamic acid, glutamin, cysteine and asparagine,
the amino acid at the position corresponding to tyrosine at position 79 is selected from the group consisting of phenylalanine and asparagine,
the amino acid at the position corresponding to glutamine at position 81 is selected from the group consisting of leucine, phenylalanine and asparagine,
the amino acid at the position corresponding to leucine at position 121 is selected from the group from the group consisting of cysteine and methionine,
the amino acid at the position corresponding to valine at position 122 is selected from the group consisting of threonine, alanine and cysteine,
the amino acid at the position corresponding to tryptophan at position 123 is selected from the group consisting of cysteine, phenylalanine, histidine, valine and serine,
the amino acid at the position corresponding to glutamic acid at position 465 is selected from the group consisting of arginine and aspartic and,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine,
the amino acid at the position corresponding to serine at position 612 is selected from the group consisting of cysteine and threonine,
the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine,
the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is cysteine,
the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is threonine,
the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to glutamic acid at position 465 is aspartic acid, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to methionine at position 78 is glutamic acid,
the amino acid at the position corresponding to trytophan at position 569 is tyrosine and the amino acid at the position corresponding to methionine at position 78 is asparagine,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to leucine at position 121 is methionine,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to tryptophan at position 123 is phenylalanine,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to tryptophan at position 123 is valine,
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to serine at position 612 is cysteine, or
the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to serine at position 612 is threonine.

2. The flavin-binding glucose dehydrogenase according to claim 1, wherein the ratio of reactivity to D-xylose to reactivity to D-glucose (Xyl/Glc (%)) and/or the ratio of reactivity to maltose to reactivity to D-glucose (Mal/Glc (%)) is decreased by 20% or more in comparison with that prior to the introduction of the substitutions.

3. A flavin-binding glucose dehydrogenase gene encoding the flavin-binding glucose dehydrogenase according to claim 1.

4. A recombinant vector containing the flavin-binding glucose dehydrogenase gene according to claim 3.

5. A host cell containing the recombinant vector according to claim 3.

6. A method for producing a flavin-binding glucose dehydrogenase, comprising the following steps:
a step for culturing the host cell according to claim 5,
a step for expressing a flavin-binding glucose dehydrogenase gene contained in the host cells, and
a step for isolating the flavin-binding glucose dehydrogenase from the culture.

7. A method for measuring glucose using the flavin-binding glucose dehydrogenase according to claim 1.

8. A glucose assay kit containing the flavin-binding glucose dehydrogenase according to claim 1.

9. A glucose sensor containing the flavin-binding glucose dehydrogenase according to claim 1.