# Patent

## Title
Peptides for the treatment of cancer

## Abstract

The present invention relates to an isolated peptide for use in the treatment of cancer consisting of 12 to 50 amino acid residues comprising—at least two beta-strands, or—at least two alpha-helices or—at least one beta-strand and at least one alpha-helix, —wherein said beta-strands and/or alpha-helices are preferably separated from each other by at least one turn, wherein the peptide has a net positive charge of +7 or more; —wherein said peptide comprises at least one peptide moiety having amino acid sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)s or the reverse sequence thereof, wherein X1 is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe), alanine (Ala), leucine (Leu) and valine (Val), X2 is a hydrophobic amino acid, preferably tryptophan (Trp), X3 is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gin), asparagine (Asn), proline (Pro), isoleucine (lie), leucine (Leu) and valine (Val), X4 is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe), tryptophan (Trp) and tyrosine (Tyr), X5 is selected from the group consisting of arginine (Arg), lysine (Lys), tyrosine (Tyr) and phenylalanine (Phe), X6 is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), valine (Val) and leucine (Leu), and X7 is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile) and serine (Ser), and wherein M is 1 or 2, Q is 1 or 2, P is 2 or 3, and S is 1, 2, 3 or 4 under the proviso that if (X5)Q is Arg-Arg S is 1; and wherein said peptide is devoid of intramolecular disulfide bonds.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a national phase application under 35 U.S.C. §371 of International Application No. PCT/EP2014/050330 filed 9 Jan. 2014, which claims priority to European Patent Application No. 13150974.7 filed 11 Jan. 2013. The entire contents of each of the above-referenced disclosures is specifically incorporated by reference herein without disclaimer.
The present invention relates to an isolated peptide exhibiting antitumoral effects.
Every year millions of people are diagnosed with cancer worldwide. Notwithstanding in the last decades much progress has been achieved in cancer therapy, nevertheless cancer remains a leading cause of death. Nowadays, surgery, chemotherapy, radiation, hormone ablation therapy and targeted therapy are the standard treatments, but in the year 2008 these were not curative in more than 50% of the cases. Furthermore, the use of these types of therapy is limited due to resistance and is accompanied by potential toxicity and diverse side effects due to inadequate specificity for tumor cells. Obviously, the discovery of new and more specific targets, together with the design of specific antitumor drugs, is one of the major interests in cancer research.
Cancer cells are often well characterized, but little is known about the plasma cell membrane, or to be more precise, the arising differences in the lipid composition in carcinogenesis. Eukaryotic plasma membranes usually comprise an overall neutral charge on the outer leaflet due to the zwitterionic phosphatidylcholine (PC) and sphingomyelin (SM). The negatively charged phospholipid phosphatidylserine (PS) together with the major part of phosphatidylethanolamine (PE) normally only assembles in the inner leaflet of eukaryotic plasma membranes. This asymmetric distribution of phospholipids is well documented and is maintained by an ATP-dependent aminophospholipid translocase. This asymmetry can get lost due to exposure of the negatively charged phosphatidylserine on the surface of cancerous and other pathological cells, apoptotic cells, as well as platelets and erythrocytes upon activation.
Based on the knowledge of PS exposure, new strategies for the design of anticancer drugs can be considered, especially cationic host defense derived peptides interacting with negatively charged phospholipids. Host defense peptides have emerged as potential alternative anticancer therapeutics offering many advantages over other therapies (Mader et al., Exp. Op. Investig. Drugs 15 (2006), 933-946). Because of their mode of action and specificity—the cell membrane being the major target—resistance and cytotoxicity are less likely to occur and thus, they are also expected to cause fewer side effects. Furthermore, these peptides mostly damage cell membranes within minutes, which would hinder formation of resistance. Host defense peptides being part of the innate immune system of many diverse species (e.g. mammals, insects, amphibians) were initially discovered because of their antimicrobial activity. Currently, the antimicrobial peptide database lists more than 100 natural host defense peptides with antitumor activity. Examples for antimicrobial peptides are disclosed in WO 2008/002165 A1.
One prominent member of anticancer peptides is bovine lactoferricin (bLFcin), which is generated from lactoferrin through pepsin cleavage. bLFcin possesses an acyclic twisted antiparallel β-sheet structure due to a disulfide bridge between two cysteine residues. This peptide is able to inhibit liver and lung metastasis in mice. In vivo studies with bLFcin on fibrosarcoma, melanoma and colon carcinoma tumors revealed massive necrosis of the tumor tissue after exposure to the peptide (Yoo et al. Jpn. J Cancer Res. 88 (1997): 184-190). Furthermore, it is known that bLFcin inhibits the tumor growth of neuroblastoma xenografts in nude rats. Clarification of the mechanism revealed that bLFcin induces apoptosis in human tumor cells through a pathway mediated by production of the intracellular ROS and activation of Ca2+/Mg2+-dependent endonucleases.
It is an object of the present invention to provide compounds and preparations which can be used to treat cancer.
Therefore the present invention relates to an isolated peptide to be used in the treatment of cancer consisting of 12 to 50 amino acid residues comprising

    
    
        at least two beta-strands, or
        at least two alpha-helices or
        at least one beta-strand and at least one alpha-helix,
    
    


wherein said beta-strands and/or alpha-helices are preferably separated from each other by at least one turn, wherein the peptide has a net positive charge of +7 or more;
wherein said peptide comprises at least one peptide moiety having amino acid sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)S or the reverse sequence thereof, wherein
X1 is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe), alanine (Ala), leucine (Leu) and valine (Val),
X2 is a hydrophobic amino acid, preferably tryptophan (Trp),
X3 is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), asparagine (Asn), proline (Pro), isoleucine (Ile), leucine (Leu) and valine (Val),
X4 is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe), tryptophan (Trp) and tyrosine (Tyr),
X5 is selected from the group consisting of arginine (Arg), lysine (Lys), tyrosine (Tyr) and phenylalanine (Phe),
X6 is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), valine (Val) and leucine (Leu), and
X7 is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile) and serine (Ser), and wherein
M is 1 or 2,
Q is 1 or 2,
P is 2 or 3, and
S is 1, 2, 3 or 4 under the proviso that if (X5)Q is Arg-Arg S is 1; and
wherein said peptide is devoid of intramolecular disulfide bonds,
preferably, said peptide being selected from the group consisting of PFWRIRIRRXRRIRIRWFP (SEQ ID. No. 128), PWRIRIRRXRRIRIRWP (SEQ ID No. 184), RWKRINRQWFFWQRNIRKWR (SEQ ID. No. 106), PFWRIRIRRPFWRIRIRR (SEQ ID. No. 125), PFFWRIRIRRPFFWRIRIRR (SEQ ID. No. 141) and PFWRIRIRRRRIRIRWFP (SEQ ID. No. 127), especially SEQ ID. Nos. 128, 184 and 106, wherein “X” is proline (Pro) and/or glycine (Gly)1-3.
It turned out that peptides having a net positive charge of +7 and comprising at least two beta-strands or at least two alpha-helices or at least one beta-strand and at least one alpha-helix and are preferably separated by at least one turn exhibit cytotoxic effects on cancerous/tumor cells in mammals. This means that the peptides of the present invention are able to affect the viability of such cells leading to their destruction. The cytotoxic effects of the peptides of the present invention are highly specific for cancerous/tumor cells. This means that these peptides affect healthy cells to a much lower extent (preferably to at least 10%, more preferably to at least 20%, even more preferably to at least 50%, in particular to at least 90 to 100%) compared to cancerous/tumor cells. This high specificity of the peptides of the present invention allows treating mammals, in particular humans, with a much higher efficacy reducing commonly known side-effects regularly described for anti-cancer compounds. The cytotoxic effect of such compounds is usually unspecific resulting in the destruction not only of cancerous/tumor cells but also of healthy cells.
The antitumour properties of the peptides according to the present invention are specifically surprising, because the present peptides are, by their definition, devoid of disulfide bonds, i.e. devoid of intramolecular S—S bonds (due to the absence of (at least) two cysteine residues in the amino acid sequence that form this bond). The molecules of the present invention have been derived from human lactoferricin (hLFcin: TKCFQWQRNMRKVRGPPVSCIKRDS (SEQ ID No. 207)), a peptide that contains two cysteins that form an intramolecular disulfide bond. One of the staring compounds of the developments of the present invention was a part of hLFcin that lacks the cysteins and wherein the methionine has been replaced by isoleucine (FQWQRNIRKVR; SEQ ID NO. 87; “PEP parent”). In contrast to other membrane active peptides, PEP parent therefore was devoid of intramolecular disulfide bonds (and even devoid of cysteine residues) that were thought to be important for membrane active (antimicrobial) function (see e.g. Harwig et al., Eur. J. Biochem. 240 (1996), 352-357). In fact, eliminating the disulfide bonds from such molecules significantly reduced membrane permeabilising activity of such peptides (Matsuzaki et al., Biochemistry 32 (1993), 11704-11710; Tamamura et al., Chem. Pharm. Bull. 43 (5) (1995), 853-858). It was known to a person with average skill in the art that disulfide bonds are of significant importance for membrane active peptides. It was therefore surprising that the peptides according to the present invention that are also membrane active, do not contain intramolecular disulfide bonds. In fact, the preferred peptide moieties which are responsible for the antitumor effect of the peptides according to the present invention are completely free of cysteine residues. The antitumour peptide according to the present invention is therefore usually cysteine-free. Cysteine residues can, however, if necessary, be used to couple the present peptide to other molecules (e.g. to carriers (e.g. carrier proteins)) which are preferably released from the peptides according to the present invention before administration or (after administration) in the body of a patient. In such cases, the disulfide bond is not located within the peptide defined by the present invention but between the peptide according to the present invention (that is defined by a continuous peptide bond connection (amino acid sequence)) and another chemical compound. That the peptides according to the present invention are devoid of intramolecular disulfide bonds is specifically surprising with regard to the fact that the peptides according to the present invention are derived from human lactoferricin (hLFcin) for which the disulfide bond has been disclosed as being essential for the antimicrobial activity and also held relevant for the antitumor activity (Gifford et al., Cell. Mol. Life Sci. 62 (2005), 2588-2598). Also for bovine lactoferricin (LFcinB), the disulfide bond was regarded as necessary for the antitumoral activity (Eliassen et al., Antican. Res. 22 (2002), 2703-2710).
This also shows that the antitumor activity of the peptides of the present invention is not dependent on a specifically stabilised (i.e. by disulfide bonds) secondary structure, but that the beta-strand and alpha-helix folding is sufficient for the peptides defined e.g. by the consensus sequences of the peptide moieties present in the peptide according to the present invention.
Preferably, the isolated peptides according to the present invention contain at least one turn. A “turn” is an element of secondary structure in polypeptides where the polypeptide chain reverses its overall direction. A “turn” may, in a structurally more precise manner, be defined as a “structural motif where the Cu atoms of two residues separated by few (usually 1 to 5) peptide bonds are in close approach (<7 Å), while the corresponding residues do not form a regular secondary structure element such as an alpha helix or beta sheet”. The turn according to the present invention may consist also of amino acids from the peptide moieties (specifically, of course, if no separate linker is located between the moieties). However, the turn according to the present invention may also be a loop (an “ω-loop being a catch-all term for a longer, extended or disordered loop without fixed internal hydrogen bonding; see also Toniolo et al., CRC Crit. Rev. Biochem. 9 (1980): 1-44).
According to the present invention the at least two beta-strands or at least two alpha-helices or the at least one beta-strand or the at least one alpha-helix are preferably separated by at least one turn resulting in peptides with alpha-helix and/or beta-strand moieties having the following general basic structures:
a) beta-strand-(turn)-beta-strand
b) alpha-helix-(turn)-alpha-helix
c) beta-strand-(turn)-alpha-helix
d) alpha-helix-(turn)-beta-strand
According to the present invention the peptides disclosed herein may also comprise 3, 4 or even 5 beta-strands or alpha-helices as peptide moieties. In such a case the beta-strands or alpha-helices of the peptide can be grouped (e.g. two beta strands are located adjacent to each other) and preferably separated by one or more (e.g. 2, 3 or 4) turns or every single strand or helix is preferably separated by one or more turns. The isolated peptide of the present invention may therefore comprise also more than one stretches having the above general basic structure.
The peptides of the present invention have a net positive charge of +7 or more (for the purpose of a formal definition for the present invention, the net positive charge can be regarded as being defined at pH 7.4 in PBS buffer (phosphate buffered saline: 20 mM NaPi, 130 mM NaCl, pH 7.4)). This means that the peptides of the present invention may have preferably a net positive charge of +8, +9, +10, +11, +12, +13, +14, +15 or even of +20. A net positive charge of at least +7 of the peptides of the present invention results in a better adsorption to the target membrane (negatively charged) and better stabilization of the secondary structure by hydrogen bridge bonds. Calculation of net charges of a peptide is performed by adding the positive charges in a polypeptide (and, if present, subtracting the negative net charges). For example, a peptide with three lysine and four arginine residues has a net positive charge of +7 at a pH below 10.5 (pK lysine ̃10.5; arginine would even be positively charged at pH 12.5!). Amidating the C-terminal carboxylate group of a peptide adds one additional positive charge (see e.g. Yang et al., J. Pep. Sci. 10 (2004), 37-46). Another possibility to arrive at the net charge Z of a peptide at a certain pH can be estimated by calculation




  Z
  =
  
    
      
        ∑
        i
      
      ⁢
      
        Ni
        ⁢
        
          
            10
            pKai
          
          
            
              10
              pH
            
            +
            
              10
              pKai
            
          
        
      
    
    -
    
      
        ∑
        j
      
      ⁢
      
        Nj
        ⁢
        
          
            10
            pH
          
          
            
              10
              pH
            
            +
            
              10
              pKaj
            
          
        
      
    
  




where Ni are the number, and pKai the pKa values, of the N-terminus and the side chains of Arginine, Lysine, and Histidine. The j-index pertain to the C-terminus and the Aspartic Acid, Glutamic Acid, Cysteine, Tyrosine amino acids.

The present peptides are preferably designed as “membrane active peptides”, i.e. peptides that have—due to their physicochemical properties—an affinity to membranes. Common properties of membrane active peptides are disclosed e.g. in Last et al., Protein Science 22 (2013), 870-882 or Wang et al. J. Biol. Chem. 114 (2010), 13726-13735). Membrane active peptides are able to perturb the structural barrier function of cell membranes, which may eventually lead to cell lysis and cell death; these peptides share two common features: amphipathicity and a net positive charge (see Rekdal at al., Journal of Biological Chemistry 287 (2012), 233-244). They share common features as cationic residues being reported to be important for the initial electrostatic interaction and hydrophobic residues being important for membrane disruption (see Lohner et al., Combinatorial Chemistry & High Throughput Screening, 2005, 8, 241-256). Presence of membrane active properties can be determined by a variety of methods. Preferred methods for verifying membrane active property according to the present invention are permeability studies (dye release) using liposomes composed of phosphatidylserine, naturally exposed by cancer cells (Riedl et al., BBA 1808 (2011) 2638-2645) and thus relevant for cancer cells, as well as membrane permeabilization of tumor cells (propidium iodide (PI)-uptake). A membrane active peptide, especially a cancer membrane active peptide of the present invention should therefore preferably exhibit at least a membrane permeabilizing activity (ANTS/DPX-release) on PS-liposomes of more than 20% at a peptide concentration of 8 μM (test according to Zweytick et al., J. Biol. Chem. 286 (2011), 21266-21276). The PI-uptake of 105 tumor cells should preferably be at least 20% after 8 hours of peptide incubation at a concentration of 20 μM of the membrane active peptide (test according to Schroder-Borm et al., J. FEBS Lett. 2005 Nov. 7; 579(27):6128-34).
Alpha helix (α-helix) is a common motif in the secondary structure of proteins, polypeptides and peptides. Alpha helices have a right-handed coiled or spiral conformation, in which every backbone N—H group donates a hydrogen bond to the backbone C═O group of the amino acid four residues earlier. The beta sheet (β-sheet) is the second form of regular secondary structure in proteins, polypeptides and peptides. Beta-sheets consist of beta strands connected laterally by at least two or three backbone hydrogen bonds, forming a generally twisted, pleated sheet. A turn is a structural motif where the Cα atoms of two residues separated by one or more peptide bonds are in close approach (approx. <7 Å), while the corresponding residues do not form a regular secondary structure element such as an alpha-helix or beta-sheet (Chou P Y et al., Annual Review of Biochemistry 47 (1978): 251-276). The secondary structure of putative membrane active isolated peptides can accurately be predicted by the online program PEP-FOLD: E.g. http://bioserv.rpbs.univ-paris-diderot.fr/PEP-FOLD/ (see Maupetit et al., Nucleic Acids Res. 37 (2009), W498-W503 and/or Thévenet et al., Nucleic Acid Res. 40 (2012), W288-W293).
The person skilled in the art is able to identify peptides exhibiting the properties as described herein using known methods. The secondary structure can be identified as described above. The net charge can be calculated by summing up the positive and negative charges of the amino acid residues present in a peptide.
One skilled in the art can easily synthesize the peptides of the present invention. Standard procedures for preparing synthetic peptides are well known in the art. Peptides of the present invention can be synthesized by commonly used methods as t-BOC or FMOC protection, preferably FMOC protection, of alpha-amino groups. Both methods involve stepwise syntheses whereby a single amino acid is added at each step starting from the carboxyl-terminus of the peptide (See, Coligan et al., Current Protocols in Immunology, Wiley Interscience, 2002, Unit 9). Peptides of the invention can also be synthesized by the solid phase peptide synthesis methods well known in the art. (Merrifield, J. Am. Chem. Soc., 85:2149, 1963), and Stewart and Young, Solid Phase Peptides Synthesis, (1969). Peptides can be synthesized using a copoly(styrene-divinylbenzene) containing 0.1-1.0 mMol amines/g polymer. On completion of chemical synthesis, the peptides can be deprotected and cleaved from the polymer by treatment with liquid HF-10% anisole for about 0.25 to 1 hour at 0° C. After evaporation of the reagents, the peptides are extracted from the polymer with 1% acetic acid solution which is then lyophilized to yield the crude material. This can typically be purified by such techniques as gel filtration on Sephadex G-15 using 5% acetic acid as a solvent, by high pressure liquid chromatography, and the like. Lyophilization of appropriate fractions of the column will yield the homogeneous peptide or peptide derivatives, which can then be characterized by such standard techniques as amino acid analysis, thin layer chromatography, high performance liquid chromatography, ultraviolet absorption spectroscopy, molar rotation, solubility, and assessed by the solid phase Edman degradation (see e.g Protein Purification, M. P. Deutscher, ed. Methods in Enzymology, Vol 182, Academic Press, 1990). Automated synthesis using FMOC solid phase synthetic methods can be achieved using an automated peptide synthesizer (Model 432A, Applied Biosystems, Inc.).
The peptides/polypeptides of the present invention can also be synthesized using a fusion protein microbial method in which an anionic carrier peptide is fused to a cationic peptide. A method for such microbial production of cationic peptides having anti-microbial activity is provided in U.S. Pat. No. 5,593,866.
The peptides of the present invention thus produced can be purified by isolation/purification methods for proteins generally known in the field of protein chemistry. More particularly, there can be mentioned, for example, extraction, recrystallization, salting out with ammonium sulfate, sodium sulfate, etc., centrifugation, dialysis, ultrafiltration, adsorption chromatography, ion exchange chromatography, hydrophobic chromatography, normal phase chromatography, reversed-phase chromatography, gel filtration method, gel permeation chromatography, affinity chromatography, electrophoresis, countercurrent distribution, etc. and combinations of these. Most effective is a method by reversed-phase high performance liquid chromatography.
The peptides of the present invention may form a salt by addition of an acid. Examples of the acid include inorganic acids (such as trifluoroacetic acid, hydrochloric acid, hydrobromic acid, phosphoric acid, nitric acid, and sulfuric acid) or organic carboxylic acids (such as acetic acid, propionic acid, maleic acid, succinic acid, malic acid, citric acid, tartaric acid, and salicylic acid), acidic sugars such as glucuronic acid, galacturonic acid, gluconic acid, ascorbic acid, etc., acidic polysaccharides such as hyaluronic acid, chondroitin sulfates, alginic acid, or organic sulfonic acids (such as methanesulfonic acid, and p-toluenesulfonic acid), and the like. Of these salts, preferred is a pharmaceutically acceptable salt.
The peptides of the present invention may form a salt with a basic substance. Examples of the salt include, for example, pharmaceutically acceptable salts selected from salts with inorganic bases such as alkali metal salts (sodium salt, lithium salt, potassium salt etc.), alkaline earth metal salts, ammonium salts, and the like or salts with organic bases, such as diethanolamine salts, cyclohexylamine salts and the like.
The term “amino acid” and “amino acid residue” as used herein means L-amino acids. However, also D-amino acids may be employed in the manufacturing of the peptides according to the present invention.
The peptide of the present invention preferably exhibits amphipathic properties. This means that the peptide of the present invention may comprise hydrophobic and hydrophilic regions. Methods to determine amphipathic properties are well known in the art.
According to the present invention the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)S (SEQ ID No. 203) or the reverse sequence thereof, wherein
X1 is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe), alanine (Ala), leucine (Leu) and valine (Val),
X2 is a hydrophobic amino acid, preferably tryptophan (Trp),
X3 is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), asparagine (Asn), proline (Pro), isoleucine (Ile), leucine (Leu) and valine (Val),
X4 is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe), tryptophan (Trp) and tyrosine (Tyr),
X5 is selected from the group consisting of arginine (Arg), lysine (Lys), tyrosine (Tyr) and phenylalanine (Phe),
X6 is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), valine (Val) and leucine (Leu), and
X7 is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile) and serine (Ser), and wherein
M is 1 or 2,
Q is 1 or 2,
P is 2 or 3, and
S is 1, 2, 3 or 4 under the proviso that if (X5)Q is Arg-Arg S is 1.
As used herein the term “reverse sequence” of an amino acid sequence means that a specific sequence is reversed. For instance, the reverse sequence of the amino acid sequence ABCDEFG is GFEDCBA.
The at least one peptide moiety has preferably an amino acid sequence selected from the group consisting of FWQRIRKVR (SEQ ID No. 1), FWQRRIRKVRR (SEQ ID No. 2), FWQRKIRKVRK (SEQ ID No. 3), FWQRNIRIRR (SEQ ID No. 4), FWQRNIRKVR (SEQ ID No. 5), FWQRNIRVR (SEQ ID No. 6), FWQRNIRKVRR (SEQ ID No. 7), FWQRNIRKVKK (SEQ ID No. 8), FWQRNIRKVRRR (SEQ ID No. 9), FWQRNIRKVKKK (SEQ ID No. 10), FWQRNIRKVRRRR (SEQ ID No. 11), FWQRNIRKVRRRI (SEQ ID No. 12), FWQRNIRKVKKKK (SEQ ID No. 13), FWQRNIRKVKKKI (SEQ ID No. 14), FWQRNIRKIR (SEQ ID No. 15), FWQRNIRKLR (SEQ ID No. 16), FWQRNIRKWR (SEQ ID No. 17), FWQRNWRKVR (SEQ ID No. 18), FWQRNFRKVR (SEQ ID No. 19), FWQRNYRKVR (SEQ ID No. 20), FWQRNIRKVS (SEQ ID No. 21), FWQRRIRIRR (SEQ ID No. 22), FWQRPIRKVR (SEQ ID No. 23), FWQRRIRKWR (SEQ ID No. 24), FWPRNIRKVR (SEQ ID No. 26), FWARNIRKVR (SEQ ID No. 27), FWIRNIRKVR (SEQ ID No. 28), FWLRNIRKVR (SEQ ID No. 29), FWVRNIRKVR (SEQ ID No. 30), FWQRNIFKVR (SEQ ID No. 31), FWQRNIYKVR (SEQ ID No. 32), FAWQRNIRKVR (SEQ ID No. 33), FLWQRNIRKVR (SEQ ID No. 35) and FVWQRNIRKVR (SEQ ID No. 36) or the reverse sequence thereof.
According to another preferred embodiment of the present invention the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′)M′-X2′-(X3′)P′-(X4′)Q′-(X5′)T′-(X6′)R′-(X7′)S′ (SEQ ID No. 204) or the reverse sequence thereof, wherein
X1′ is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe) and isoleucine (Ile),
X2′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X3′ is selected from the group consisting of glycine (Gly), asparagine (Asn), isoleucine (Ile) and phenylalanin (Phe),
X4′ is isoleucine (Ile) or tryptophan (Trp),
X5′ is arginine (Arg) or lysine (Lys),
X6′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp) and valine (Val) and
X7′ is arginine (Arg), and wherein
M′ is 1 or 2,
T′ is 1 or 2,
R′ is 0 or 1,
P′ is 1, 2 or 3,
Q′ is 1, and
S′ is 0, 1 or 2.
The at least one peptide moiety in the peptide of the present invention may have an amino acid sequence selected from the group consisting of FWRIRKWR (SEQ ID No. 37), FWRIRKVR (SEQ ID No. 38), FWRWRR (SEQ ID No. 39), FWRRWRR (SEQ ID No. 40), FWRRWIRR (SEQ ID No. 41), FWRGWRIRR (SEQ ID No. 42), FWRRFWRR (SEQ ID No. 43), FWRWRWR (SEQ ID No. 44), FWRIWRWR (SEQ ID No. 45), FWRIWRIWR (SEQ ID No. 46), FWRNIRKWR (SEQ ID No. 47) and FWRRRIRIRR (SEQ ID No. 48) or the reverse sequence thereof.
According to a further preferred embodiment of the present invention the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′′)M′′-X2′′-(X3′′)P′′-(X4′′)Q′′-(X5′′)R′′-(X6′′)S′′ (SEQ ID No. 205) or the reverse sequence thereof, wherein
X1′′ is a hydrophobic amino acid, preferably selected from the group consisting of proline (Pro) and phenylalanine (Phe),
X2′′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X3′′ is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), lysine (Lys), tryptophan (Trp) and isoleucine (Ile),
X4′′ is selected from the group consisting of arginine (Arg) and aspartate (Asp),
X5′′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), phenylalanine (Phe), valine (Val) and leucine (Leu), and
X6′′ is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile), serine (Ser) and aspartate (Asp), and wherein
M′′ is 0, 1, 2 or 3,
Q′′ is 0, 1, 2 or 3,
R′′ is 1 or 2,
P′′ is 1, 2 or 3, and
S′′ is 1, 2 or 3.
The at least one peptide moiety has preferably an amino acid sequence selected from the group consisting of PFWRWRIWR (SEQ ID No. 50), PFWRIRIRR (SEQ ID No. 51), PFWRQRIRR (SEQ ID No. 52), PFWRARIRR (SEQ ID No. 53), PFWRKRIRR (SEQ ID No. 54), PFWRKRLRR (SEQ ID No. 55), PFWRKRWRR (SEQ ID No. 56), PFWRRRIRR (SEQ ID No. 57), PFWRRRWRR (SEQ ID No. 58), PFWRIRIRRD (SEQ ID No. 59), PFFWRIRIRR (SEQ ID No. 60), PWRIRIRR (SEQ ID No. 61), PFWRRQIRR (SEQ ID No. 81), PFWRKKLKR (SEQ ID No. 82), PWRRIRR (SEQ ID No. 83), PWRRKIRR (SEQ ID No. 84) and PFWRRIRIRR (SEQ ID No. 85) or the reverse sequence thereof.
According to a preferred embodiment of the present invention the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′′′)M′′′-(X2′′′)O′′′-X3′′′-(X4′′′)P′′′—(X5′′′)Q′′′-(X6′′′)T′′′-(X7′′′)R′′′-(X8′′′)S′′′ (SEQ ID No. 206) or the reverse sequence thereof, wherein
X1′′′ is a hydrophobic amino acid, preferably selected from the group consisting of proline (Pro) and phenylalanine (Phe),
X2′′′ is a basic amino acid, preferably arginine (Arg),
X3′′′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X4′′′ is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), asparagine (Asn) and lysine (Lys),
X5′′′ is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe) and tryptophan (Trp),
X6′′′ is selected from the group consisting of glutamine (Gln), arginine (Arg) and asparagine (Asn),
X7′′′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp) and phenylalanine (Phe), and
X8′′′ is arginine (Arg), and wherein
M′′′ is 0, 1, 2 or 3,
T′′′ is 0, 1, 2 or 3,
O′′′ is 0 or 1,
P′′′ is 1, 2 or 3,
Q′′′ is 1 or 2, and
R′′′ and S′′′ are 0, 1 or 2.
The at least one peptide may have an amino acid sequence selected from the group consisting of FWRNIRIRR (SEQ ID No. 72), FWQRIRIRR (SEQ ID No. 73), FWRWRIWR (SEQ ID No. 74), FWRIRIRR (SEQ ID No. 75), FWRNIRIWRR (SEQ ID No. 76) and FWRNIRIRR (SEQ ID No. 77) or the reverse sequence thereof.
The isolated peptide comprises preferably at least one peptide moiety having an amino acid sequence selected from the group consisting of RFWQRNIRKVRR (SEQ ID No. 62), RFWQRNIRKYR (SEQ ID No. 63), PFWQRNIRKWR (SEQ ID No. 64), RFRWQRNIRKYRR (SEQ ID No. 65), RWKRINRQWF (SEQ ID No. 66), KRFCFKK (SEQ ID No. 67), KRFSFKKC (SEQ ID No. 68), KRWSWKK (SEQ ID No. 69), FRFSFKK (SEQ ID No. 70), RRFWFRR (SEQ ID No. 71), RFWQRNIRIRR (SEQ ID No. 78), RWQRNIRIRR (SEQ ID No. 79) and RRWFWRR (SEQ ID No. 86) or the reverse sequence thereof.
According to a further embodiment of the present invention the isolated peptide comprises at least one peptide moiety having an amino acid sequence selected from the group consisting of FIWQRNIRKVR (SEQ ID No. 34), FIWRWRWR (SEQ ID No. 49) and RRIRINRQWF (SEQ ID No. 80) or the reverse sequence thereof.
The isolated peptide of the present invention may comprise a single peptide moiety or the reverse sequence thereof as defined above. However, the isolated peptide may also comprise a multiplicity (at least two, at least three, at least four etc.) of said single peptide moieties or peptide moieties having the reversed sequence thereof. According to a very particular preferred embodiment of the present invention the isolated peptide comprises at least two, most preferably two, of the aforementioned peptide moieties with the sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)S or the reverse sequence thereof.
According to a particularly preferred embodiment of the present invention the peptide of the present invention is selected from the peptides as shown in Table 1 having at least two beta-strands, or at least two alpha-helices or at least one beta-strand and at least one alpha-helix, said beta-strands and/or alpha-helices optionally being separated from each other by at least one turn. Preferred are those peptides having an amino acid sequence selected from the group consisting of SEQ ID. No. 89, SEQ ID. No. 91, SEQ ID. No. 93, SEQ ID. No. 95, SEQ ID. No. 97, SEQ ID. No. 98, SEQ ID. No. 99, SEQ ID. No. 101, SEQ ID. No. 103, SEQ ID. No. 105, SEQ ID. No. 106, SEQ ID. No. 107, SEQ ID. No. 109, SEQ ID. No. 111, SEQ ID. No. 114, SEQ ID. No. 116, SEQ ID. No. 117, SEQ ID. No. 118, SEQ ID. No. 120, SEQ ID. No. 122, SEQ ID. No. 123, SEQ ID. No. 124, SEQ ID. No. 125, SEQ ID. No. 126, SEQ ID. No. 127, SEQ ID. No. 128, SEQ ID. No. 129, SEQ ID. No. 130, SEQ ID. No. 131, SEQ ID. No. 132, SEQ ID. No. 134, SEQ ID. No. 136, SEQ ID. No. 137, SEQ ID. No. 138, SEQ ID. No. 139, SEQ ID. No. 140, SEQ ID. No. 141, SEQ ID. No. 142, SEQ ID. No. 143, SEQ ID. No. 144, SEQ ID. No. 145, SEQ ID. No. 146, SEQ ID. No. 148, SEQ ID. No. 149, SEQ ID. No. 150, SEQ ID. No. 156, SEQ ID. No. 153, SEQ ID. No. 154, SEQ ID. No. 156, SEQ ID. No. 158, SEQ ID. No. 160, SEQ ID. No. 162, SEQ ID. No. 164, SEQ ID. No. 166, SEQ ID. No. 168, SEQ ID. No. 170, SEQ ID. No. 172, SEQ ID. No. 174, SEQ ID. No. 176, SEQ ID. No. 178, SEQ ID. No. 180, SEQ ID. No. 181, SEQ ID. No. 182, SEQ ID. No. 183, SEQ ID. No. 184, SEQ ID. No. 186, SEQ ID. No. 188, SEQ ID. No. 190, SEQ ID. No. 192, SEQ ID. No. 194, SEQ ID. No. 195, SEQ ID. No. 197, SEQ ID. No. 199, SEQ ID. No. 200, SEQ ID. No. 201 and SEQ ID. No. 202. The variable “X” within these sequences can be 1 to 3 (i.e. 1, 2 or 3) glycine or proline residues, preferably 1 proline residue. Particularly preferred peptides are those having a sequence selected from the group consisting of SEQ ID. No. 125, SEQ ID. No. 126, SEQ ID. No. 127, SEQ ID. No. 128, SEQ ID. No. 105, SEQ ID. No. 106, SEQ ID. No. 107, SEQ ID. No. 174, SEQ ID. No. 176, SEQ ID. No. 141, SEQ ID. No. 142, SEQ ID. No. 143, SEQ ID. No. 144, SEQ ID. No. 181, SEQ ID. No. 182, SEQ ID. No. 183 and SEQ ID. No. 184.
According to a preferred embodiment of the present invention the peptide of the present invention has/comprises/consists of a sequence selected from the group consisting of SEQ ID. No. 125, SEQ ID. No. 127, SEQ ID. No. 128, SEQ ID. No. 106 and SEQ ID. No. 141 and No. 184. For example, SEQ ID. Nos. 128, 184 and 106, especially Nos. 128 and 184, are specifically suitable for cancer treatment, especially for the treatment of glioblastoma and malignant melanoma.
Of course the isolated peptide of the present invention may also comprise a combination of at least two of the aforementioned peptides.
According to a preferred embodiment of the present invention at least two peptides having an amino acid sequence as defined herein or the reverse sequence thereof are fused directly or via a linker, wherein said linker is preferably part of the turn, to each other.
According to a further preferred embodiment of the present invention said isolated peptide comprises at least two, preferably two, peptide moieties with the sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)S or the reverse sequence thereof (“retro” sequence) having the same amino acid sequence and being selected from the peptide moieties having an amino acid sequence as defined above or the reverse sequence thereof, wherein the at least two peptide moieties are fused directly or via a linker to each other (e.g. the “reverse” or “retro” sequence of X1X2X3X4 is X4X3X2X1; e.g. “retro” sequence of PWRIRIRR is RRIRIRWP).
Said isolated peptide may comprise at least two, preferably two, peptide moieties, wherein an at least one first peptide moiety has an amino acid sequence as defined above and the at least one second peptide moiety is the reverse sequence thereof, wherein the at least two peptide moieties are fused directly or via a linker to each other.
The linker comprises preferably 1 to 10, preferably 1 to 8, more preferably 1 to 5, even more preferably 1 to 3, amino acid residues.
According to a particularly preferred embodiment of the present invention the linker, being preferably part of the turn, comprises or consists of proline and/or glycine, preferably proline.
The isolated peptide of the present invention or a pharmaceutical preparation comprising at least one of said isolated peptides can be used to treat cancer of solid and non-solid tumors, including metastases, whereby the cancer is preferably selected from the group consisting of melanoma, rhabdomyosarcoma, glioblastoma, colorectal cancer, breast cancer, lymphoma, prostate cancer, pancreatic cancer, renal cancer, ovarian cancer and lung cancer. Particularly preferred cancers to be treated with the peptides of the present invention are glioblastoma and melanoma, preferably malignant melanoma. The peptides of the present invention are preferably administered to a patient in need thereof in an amount of 100 μg/kg body weight to 100 mg/kg body weight, preferably 1 mg/kg body weight to 50 mg/kg body weight, more preferably 5 mg/kg body weight to 15 mg/kg body weight, in particular 10 mg/kg body weight. Furthermore the peptides of the present invention are preferably administered daily (e.g. three times a day, twice a day or once a day), every 2rd, every 3rd, every 4th or every 5th day.
In order to obtain a pharmaceutical composition with even better anti-cancer or anti-tumor activity additional agents exhibiting similar properties as the peptides according to the present invention are added. Of course it is also possible to add agents with activities other than the peptides according to the present invention. These substances may be helpful in increasing the bioavailability such as for example increasing the stability of the peptides or their delivery.
Such compositions according to the present invention may preferably further comprise a pharmaceutically acceptable excipient.
The pharmaceutical composition of the present invention may consist of the peptide of the present invention alone or may be in the form of a composition comprising the peptide of the present invention and a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier which can be used is not limited particularly and includes an excipient, a binder, a lubricant, a colorant, a disintegrant, a buffer, an isotonic agent, a preservative, an anesthetic, and the like which can be used in a medical field.
The composition of the present invention can be administered, depending on the cancer to be treated locally or systemically by injection (subcutaneous, intracutaneous, intravenous, intraperitoneal, etc.), eye dropping, instillation, percutaneous administration, oral administration, inhalation, etc. The peptides of the present invention can also be directly injected into the tumor/cancer to be treated.
Also, the dosage form such as injectable preparations (solutions, suspensions, emulsions, solids to be dissolved when used, etc.), tablets, capsules, granules, powders, liquids, liposome inclusions, ointments, gels, external powders, sprays, inhalating powders, eye drops, eye ointment, suppositories, pessaries, and the like can be appropriately selected depending on the administration method, and the composition of the present invention can be accordingly formulated.
Another aspect of the present invention relates to the use of a peptide as defined above for the manufacturing of a medicament for treating cancer in a mammal, in particular in a human patient.
A further aspect of the present invention relates to a method for treating a mammal, in particular a human patient, suffering from cancer by administering to said mammal an effective amount of a peptide as defined above.
As used herein, the term “therapeutically effective amount” or “effective amount” means that to a mammal an amount of the peptide of the present invention is administered which allows the reduction of the tumor cells within the body of at least 10%, preferably at least 20%, more preferably at least 50%, and more preferably sufficient to reduce by 90%. Generally, the dosage will vary with age, condition and sex, and can be determined by one skilled in the art. The dosage can be adjusted by the individual physician in the event of any contraindications. In any event, the effectiveness of treatment can be determined by monitoring the presence of the cancer cells within the body.
According to a particularly preferred embodiment of the present invention the peptide of the present invention has/comprises/consists of a sequence selected from the group consisting of SEQ ID. No. 125, SEQ ID. No. 127, SEQ ID. No. 128, SEQ ID. No. 106 and SEQ ID. No. 141 and is used in the treatment of glioblastoma and melanoma, preferably malignant melanoma. The peptide of the present invention can be administered by directly injecting the peptide into the tumor/cancer.
Preferably the present invention is defined as in the following embodiments:
1. An isolated peptide to be used in the treatment of cancer consisting of 12 to 50 amino acid residues comprising

    
    
        at least two beta-strands, or
        at least two alpha-helices or
        at least one beta-strand and at least one alpha-helix,

wherein said beta-strands and/or alpha-helices are preferably separated from each other by at least one turn, wherein the peptide has a net positive charge of +7 or more.

    
    


2. Peptide for use according to embodiment 1, wherein the peptide comprises at least 5 hydrophobic amino acid residues.
3. Peptide for use according to embodiment 1 or 2, wherein the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1)M-X2-(X3)P-X4-(X5)Q-X6-(X7)S or the reverse sequence thereof, wherein
X1 is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe), alanine (Ala), leucine (Leu) and valine (Val),
X2 is a hydrophobic amino acid, preferably tryptophan (Trp),
X3 is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), asparagine (Asn), proline (Pro), isoleucine (Ile), leucine (Leu) and valine (Val),
X4 is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe), tryptophan (Trp) and tyrosine (Tyr),
X5 is selected from the group consisting of arginine (Arg), lysine (Lys), tyrosine (Tyr) and phenylalanine (Phe),
X6 is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), valine (Val) and leucine (Leu), and
X7 is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile) and serine (Ser), and wherein
M is 1 or 2,
Q is 1 or 2,
P is 2 or 3, and
S is 1, 2, 3 or 4 under the proviso that if (X5)Q is Arg-Arg S is 1.
4. Peptide for use according to embodiment 3, wherein the at least one peptide moiety has an amino acid sequence selected from the group consisting of FWQRIRKVR (SEQ ID No. 1), FWQRRIRKVRR (SEQ ID No. 2), FWQRKIRKVRK (SEQ ID No. 3), FWQRNIRIRR (SEQ ID No. 4), FWQRNIRKVR (SEQ ID No. 5), FWQRNIRVR (SEQ ID No. 6), FWQRNIRKVRR (SEQ ID No. 7), FWQRNIRKVKK (SEQ ID No. 8), FWQRNIRKVRRR (SEQ ID No. 9), FWQRNIRKVKKK (SEQ ID No. 10), FWQRNIRKVRRRR (SEQ ID No. 11), FWQRNIRKVRRRI (SEQ ID No. 12), FWQRNIRKVKKKK (SEQ ID No. 13), FWQRNIRKVKKKI (SEQ ID No. 14), FWQRNIRKIR (SEQ ID No. 15), FWQRNIRKLR (SEQ ID No. 16), FWQRNIRKWR (SEQ ID No. 17), FWQRNWRKVR (SEQ ID No. 18), FWQRNFRKVR (SEQ ID No. 19), FWQRNYRKVR (SEQ ID No. 20), FWQRNIRKVS (SEQ ID No. 21), FWQRRIRIRR (SEQ ID No. 22), FWQRPIRKVR (SEQ ID No. 23), FWQRRIRKWR (SEQ ID No. 24), FWPRNIRKVR (SEQ ID No. 26), FWARNIRKVR (SEQ ID No. 27), FWIRNIRKVR (SEQ ID No. 28), FWLRNIRKVR (SEQ ID No. 29), FWVRNIRKVR (SEQ ID No. 30), FWQRNIFKVR (SEQ ID No. 31), FWQRNIYKVR (SEQ ID No. 32), FAWQRNIRKVR (SEQ ID No. 33), FLWQRNIRKVR (SEQ ID No. 35) and FVWQRNIRKVR (SEQ ID No. 36) or the reverse sequence thereof.
5. Peptide for use according to any one of embodiments 1 to 4, wherein the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′)M′-X2′-(X3′)-(X4′)Q′-(X5′)T′-(X6′)R′-(X7′)S′ or the reverse sequence thereof, wherein
X1′ is a hydrophobic amino acid, preferably selected from the group consisting of phenylalanine (Phe) and isoleucine (Ile),
X2′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X3′ is selected from the group consisting of glycine (Gly), asparagine (Asn), isoleucine (Ile) and phenylalanin (Phe),
X4′ is isoleucine (Ile) or tryptophan (Trp),
X5′ is arginine (Arg) or lysine (Lys),
X6′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp) and valine (Val) and
X7′ is arginine (Arg), and wherein
M′ is 1 or 2,
T′ is 1 or 2,
R′ is 0 or 1,
P′ is 1, 2 or 3,
Q′ is 1, and
S′ is 0, 1 or 2.
6. Peptide for use according to embodiment 5, wherein the at least one peptide moiety has an amino acid sequence selected from the group consisting of FWRIRKWR (SEQ ID No. 37), FWRIRKVR (SEQ ID No. 38), FWRWRR (SEQ ID No. 39), FWRRWRR (SEQ ID No. 40), FWRRWIRR (SEQ ID No. 41), FWRGWRIRR (SEQ ID No. 42), FWRRFWRR (SEQ ID No. 43), FWRWRWR (SEQ ID No. 44), FWRIWRWR (SEQ ID No. 45), FWRIWRIWR (SEQ ID No. 46), FWRNIRKWR (SEQ ID No. 47) and FWRRRIRIRR (SEQ ID No. 48) or the reverse sequence thereof.
7. Peptide for use according to any one of embodiments 1 to 6, wherein the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′′)M′′-X2′′-(X3′′)P′′-(X4′′)Q′′-(X5′′)R′′-(X6′′)S′′ or the reverse sequence thereof, wherein
X1′′ is a hydrophobic amino acid, preferably selected from the group consisting of proline (Pro) and phenylalanine (Phe),
X2′′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X3′′ is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), lysine (Lys), tryptophan (Trp) and isoleucine (Ile),
X4′′ is selected from the group consisting of arginine (Arg) and aspartate (Asp),
X5′′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp), phenylalanine (Phe), valine (Val) and leucine (Leu), and
X6′′ is selected from the group consisting of arginine (Arg), lysine (Lys), isoleucine (Ile), serine (Ser) and aspartate (Asp), and wherein
M′′ is 0, 1, 2 or 3,
Q′′ is 0, 1, 2 or 3,
R′′ is 1 or 2,
P′′ is 1, 2 or 3, and
S′′ is 1, 2 or 3.
8. Peptide for use according to embodiment 7, wherein the at least one peptide moiety has an amino acid sequence selected from the group consisting of PFWRWRIWR (SEQ ID No. 50), PFWRIRIRR (SEQ ID No. 51), PFWRQRIRR (SEQ ID No. 52), PFWRARIRR (SEQ ID No. 53), PFWRKRIRR (SEQ ID No. 54), PFWRKRLRR (SEQ ID No. 55), PFWRKRWRR (SEQ ID No. 56), PFWRRRIRR (SEQ ID No. 57), PFWRRRWRR (SEQ ID No. 58), PFWRIRIRRD (SEQ ID No. 59), PFFWRIRIRR (SEQ ID No. 60), PWRIRIRR (SEQ ID No. 61), PFWRRQIRR (SEQ ID No. 81), PFWRKKLKR (SEQ ID No. 82), PWRRIRR (SEQ ID No. 83), PWRRKIRR (SEQ ID No. 84) and PFWRRIRIRR (SEQ ID No. 85) or the reverse sequence thereof.
9. Peptide for use according to any one of embodiments 1 to 8, wherein the isolated peptide comprises at least one peptide moiety having amino acid sequence (X1′′′)M′′′-(X2′′′)O′′′-X3′′′-(X4′′′)P′′′-(X5′′′)Q′′′-(X6′′′)T′′′-(X7′′′)R′′′-(X8′′′)S′′′ or the reverse sequence thereof, wherein
X1′′′ is a hydrophobic amino acid, preferably selected from the group consisting of proline (Pro) and phenylalanine (Phe),
X2′′′ is a basic amino acid, preferably arginine (Arg),
X3′′′ is a hydrophobic amino acid, preferably tryptophan (Trp),
X4′′′ is selected from the group consisting of alanine (Ala), arginine (Arg), glutamine (Gln), asparagine (Asn) and lysine (Lys),
X5′′′ is selected from the group consisting of isoleucine (Ile), phenylalanine (Phe) and tryptophan (Trp),
X6′′′ is selected from the group consisting of glutamine (Gln), arginine (Arg) and asparagine (Asn),
X7′′′ is a hydrophobic amino acid, preferably selected from the group consisting of isoleucine (Ile), tryptophan (Trp) and phenylalanine (Phe), and
X8′′′ is arginine (Arg), and wherein
M′′′ is 0, 1, 2 or 3,
T′′′ is 0, 1, 2 or 3,
O′′′ is 0 or 1,
P′′′ is 1, 2 or 3,
Q′′′ is 1 or 2, and
R′′′ and S′′′ are 0, 1 or 2.
10. Peptide for use according to embodiment 9, wherein the at least one peptide moiety has an amino acid sequence selected from the group consisting of FWRNIRIRR (SEQ ID No. 72), FWQRIRIRR (SEQ ID No. 73), FWRWRIWR (SEQ ID No. 74), FWRIRIRR (SEQ ID No. 75), FWRNIRIWRR (SEQ ID No. 76) and FWRNIRIRR (SEQ ID No. 77) or the reverse sequence thereof.
11. Peptide for use according to any one of embodiments 1 to 10, wherein the isolated peptide comprises at least one peptide moiety having an amino acid sequence selected from the group consisting of RFWQRNIRKVRR (SEQ ID No. 62), RFWQRNIRKYR (SEQ ID No. 63), PFWQRNIRKWR (SEQ ID No. 64), RFRWQRNIRKYRR (SEQ ID No. 65), RWKRINRQWF (SEQ ID No. 66), KRFCFKK (SEQ ID No. 67), KRFSFKKC (SEQ ID No. 68), KRWSWKK (SEQ ID No. 69), FRFSFKK (SEQ ID No. 70), RRFWFRR (SEQ ID No. 71), RFWQRNIRIRR (SEQ ID No. 78), RWQRNIRIRR (SEQ ID No. 79) and RRWFWRR (SEQ ID No. 86) or the reverse sequence thereof.
12. Peptide for use according to any one of embodiments 1 to 11, wherein the isolated peptide comprises at least one peptide moiety having an amino acid sequence selected from the group consisting of FIWQRNIRKVR (SEQ ID No. 34), FIWRWRWR (SEQ ID No. 49) and RRIRINRQWF (SEQ ID No. 80) or the reverse sequence thereof.
13. Peptide for use according to any one of embodiments 1 to 12, wherein at least two peptide moieties having an amino acid sequence as defined in any one of embodiments 4 to 13 or the reverse sequence thereof are fused directly or via a linker to each other.
14. Peptide for use according to any one of embodiments 1 to 12, wherein said isolated peptide comprises at least two, preferably two, peptide moieties having the same amino acid sequence and being selected from the peptide moieties having an amino acid sequence as defined in any one of embodiments 4 to 13 or the reverse sequence thereof, wherein the at least two peptide moieties are fused directly or via a linker to each other.
15. Peptide for use according to any one of embodiments 1 to 12, wherein said isolated peptide comprises at least two, preferably two, peptide moieties, wherein an at least one first peptide moiety has an amino acid sequence as defined in any one of embodiments 4 to 13 and the at least one second peptide moiety is the reverse sequence thereof, wherein the at least two peptide moieties are fused directly or via a linker to each other.
16. Peptide for use according to any one of embodiments 13 to 15, wherein the linker comprises 1 to 10, preferably 1 to 8, more preferably 1 to 5, even more preferably 1 to 3, amino acid residues.
17. Peptide for use according to any one of embodiments 13 to 16, wherein the linker comprises proline and/or glycine residues.
18. Peptide for use according to any one of embodiments 13 to 17, wherein the linker consists of one, two or three, preferably one, proline residue.
19. Peptide for use according to any one of embodiments 1 to 18, wherein the cancer is selected from solid and non-solid tumors, including metastases.
20. Peptide for use according to any one of embodiments 1 to 19, wherein the cancer is selected from the group consisting of melanoma, rhabdomyosarcoma, glioblastoma, colorectal cancer, breast cancer, lymphoma, prostate cancer, pancreatic cancer, renal cancer, ovarian cancer and lung cancer.
21. Use of a peptide as defined in any one of embodiments 1 to 18 for the manufacturing of a medicament for treating cancer in a mammal, in particular in a human patient.
22. Method for treating a mammal, in particular a human patient, suffering from cancer by administering to said mammal an effective amount of a peptide as defined in any one of embodiments 1 to 18.
The present invention is further illustrated by the following figures and examples, however, without being restricted thereto.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the secondary structures of PEP-322 (A) and R-DIM-P-PEP-322 (B) in Hepes buffer (first bar) or presence of SDS and DPC at peptide to surfactant ratios of 1:25 and 1:100, determined using CD spectroscopy. Bottom shows α-helical content in dark gray; second from bottom shows β-sheet in light grey; third from bottom shows turns in middle grey; random coil structures are shown in dark grey at the top.
FIG. 2 shows the secondary structures of DIM-PEP-318 (A) and R-DIM-P-PEP-322 (B) in the absence and presence of SDS and DPC at peptide to surfactant ratios of 1:25 and 1:100, determined using CD spectroscopy. Bottom shows α-helical content in dark gray; second from bottom shows β-sheet in light grey; third from bottom shows turns in middle grey; random coil structures are shown in dark grey at the top.
FIG. 3 shows peptide toxicity-PI-uptake of cancer and non-cancer cell lines: (A) Concentration dependent cytotoxic activity of PEP-322 (●) and R-DIM-P-PEP-322 (▾) against melanoma cell line SBcl-2 after 8 hours of incubation with peptides; (B) Concentration dependent cytotoxic activity against primary cultures of differentiated non-tumorigenic melanocytes after 8 hour of incubation with peptides; (C) specificity of peptides at 20 μM peptide concentration after 8 h of incubation displayed as PI-uptake ratio of SBcl-2 vs. melanocytes and WM164 vs. melanocytes.
FIG. 4 shows PI-uptake of various cancerous and non-cancerous cell lines upon incubation with 20 μM peptide: Time dependent cytotoxic activity of PEP-322 (●), R-DIM-P-PEP-322 (▾), PEP-318 (Δ) and DIM-PEP-318 (▪) against melanoma cell line SBcl-(A), melanoma metastasis WM164 (B), Rhabdomyosarcoma cell line TE671 (C), differentiated non-tumorigenic melanocytes cell lines (D) and normal human dermal fibroblast cell line NHDF (E) at 20 μM peptide concentration is shown.
FIG. 5 shows cytotoxicity of peptides after 8 h of incubation against SBcl-2 melanoma cell line, WM164 melanoma metastasis, TE671 rhabdomyosarcoma cell line, differentiated non-tumorigenic melanocyte cell line and NHDF normal human dermal fibroblast cell line.
FIG. 6 shows cytotoxic activity of PEP-322 (A), R-DIM-P-PEP-322 (B) and DIM-PEP-318 (C) determined by MTS cell proliferation assay against melanoma cell line SBcl-2 melanoma metastasis WM164 and non-cancer human dermal fibroblasts (NHDF). Cells were kept in the appropriate medium during incubation time of 24 h.
FIG. 7 shows spectrofluorimetric analysis of Caspase 3/7-activity of melanoma cell line SBcl-2 upon incubation of 4 h with different concentrations (5 μM, 10 μM, 20 μM, 40 μM, 80 μM) of peptide R-DIM-P-PEP-322 (black) and DIM-PEP-318 (white).
FIGS. 8 to 11 show cytotoxicity of peptides after 1 h, 2 h, 4 h and 8 h of incubation, respectively, against SBcl-2 melanoma cell line, WM164 melanoma metastasis, TE671 rhabdomyosarcoma cell line, differentiated non-tumorigenic melanocyte cell line and NHDF normal human dermal fibroblast cell line.
FIG. 12 shows cytotoxicity of specific peptides R-DIM-P-PEP-322 and R-DIM-PEP-316 and less specific peptides R-DIM-PEP-337 and DIM-PEP-318 and weakly active human Lactoferricin (hLFcin) (37-61), which contains two cysteins (Cys3 and Cys20) connected via a disulfide bond, after 8 h of incubation, respectively, against U87 mg glioblastoma cell line, A375 melanoma cell line and NHDF-c normal human dermal fibroblast cell line and PEP-FOLD secondary structure predictions of peptides.
FIG. 13 shows secondary structure predictions of the peptides PEP-322 (A), R-DIM-P-PEP-322 (B), R-DIM-P-PEP-334 (C), R-DIM-PEP-316 (●), R-DIM-PEP-337 (E), DIM-PEP-324 (F) and DIM-318 (G), analyzed by the program PEP-FOLD.
FIG. 14 shows cytotoxicity of peptides R-DIM-P-PEP-316, DIM-PEP-317, DIM-PEP-318, DIM-PEP-322, R-DIM-PEP-322, R-DIM-P-PEP-322, R-DIM-PEP-323, DIM-PEP-324, R-DIM-P-PEP-324, R-DIM-P-PEP-330, R-DIM-P-PEP-332, R-DIM-P-PEP-334 and R-DIM-PEP-337 and human Lactoferricin (hLFcin)(37-61) after 1 h, 2 h, 4 h and h of incubation, respectively, against A375 melanoma cell line.
FIG. 15 shows cytotoxicity of peptides R-DIM-P-PEP-316, DIM-PEP-317, DIM-PEP-318, DIM-PEP-322, R-DIM-PEP-322, R-DIM-P-PEP-322, R-DIM-PEP-323, DIM-PEP-324, R-DIM-P-PEP-324, R-DIM-P-PEP-330, R-DIM-P-PEP-332, R-DIM-P-PEP-334 and R-DIM-PEP-337 and human Lactoferricin (hLFcin)(37-61) after 1 h, 2 h, 4 h and h of incubation, respectively, against U87 mg glioblastoma cell line.



EXAMPLES
Experimental Procedures
Materials and Peptide Synthesis
1,2-Dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC), 1-Hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (POPC), 1,2-Dihexadecanoyl-sn-glycero-3-phospho-L-serine (Na-salt) (DPPS) and 1-Hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine (Na-salt) (POPS) were purchased from Avanti Polar Lipids, Inc. (USA), and used without further purification. Stock solutions of DPPC and POPC were prepared in CHCl3/CH3OH (2:1, v/v), stock solutions of DPPS and POPS were prepared in CHCl3/CH3OH (9:1, v/v) and stored at −18° C.
The amidated peptides PEP-322 (PFWRIRIRR-NH2 (SEQ ID NO: 51), M=1298.6 g/mole, DIM-PEP-322 (2580.2), M=g/mole, R-DIM-PEP-322 (2580.2), M=g/mole, R-DIM-P-PEP-322 (PFWRIRIRRPRRIRIRWFP-NH2 (SEQ ID NO: 128), M=2677.4 g/mole), PEP-318 (FWQRRIRRWRR-NH2 (SEQ ID NO: 112), M=1715.0 g/mol), DIM-PEP-318 (FWQRRIRRWRRFWQRRIRRWRR-NH2 (SEQ ID NO: 113), M=3413.1 g/mol), PEP-324 (PFFWRIRIRR-NH2 (SEQ ID NO: 60), M=1445.8 g/mol), DIM-PEP-324 (PFFWRIRIRRPFFWRIRIRR-NH2 (SEQ ID NO: 141), M=2874.6 g/mol), PEP-316 (RWKRINRQWF-NH2 (SEQ ID NO: 66), M=1488.8 g/mol) and R-DIM-PEP-316 (RWKRINRQWFFWQRNIRKWR-NH2 (SEQ ID NO: 106), M=2960.5 g/mol) were purchased from NeoMPS, Inc. (San Diego, Calif., USA). (Human lactoferricin (hLFcin) 37-61) (TKCFQWQRNMRKVRGPPVSCIKRDS (SEQ ID No. 207), M=3020.5 g/mol) was purchased from Anaspec, Inc (Fremont, Calif., USA). The purities were >95% as determined by RP-HPLC. Peptides were dissolved in acetic acid (0.1%, v/v) at a concentration of 3 mg/ml. Peptide solutions were stored at 4° C. and concentrations were determined photometrically at 280 nm.
ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) and DPX (p-xylene-bis-pyridinium bromide) used for permeability studies were purchased from Molecular Probes (Eugene, Oreg.).
The peptides of the present invention, which have been used in part in the present example are indicated in Table 1:
Table 1:
List of sequences of peptide moieties and isolated peptides (-retro); the present invention includes all sequences in isolated peptide (The term “DIM-” used hereinafter was used to assign a “di-moiety” peptide according to the present invention) (peptide moietysequence+peptide moietysequence) isolated peptide retro (R-DIM-) (peptide moiety sequence+peptide moiety retro sequence), isolated peptide with linker (DIM-X-) (peptide moiety sequence+X+peptide moiety sequence) isolated peptide retro with linker X (R-DIM-X-) (peptide moiety sequence+X+peptide moiety retro sequence) (X=(Pro and/or, preferably or, Gly)1-3, whereby X is preferably a single proline residue); the present invention includes as well trimer and tetrameric derivatives termed as polymeric versions of the isolated peptide according to the present invention, although the more consequent wording would be “poly-moietic peptide” (“tri-moiety peptide”, “tetra-moiety peptide”, “penta-moiety peptide”, etc.); —represents a peptide bond between the peptide moieties and between the peptides and the linker; “retro sequence” or “reverse sequence” refers to an amino acid sequence comprising a sequence which has a reverse order than the amino acid sequence from which it is derived from (e.g. the retro sequence of ABCDE is EDCBA).














SEQ


Designation
Sequence
ID No.

















PEP (parent)
FQWQRNIRKVR
87


DIM-PEP
FQWQRNIRKVR - FQWQRNIRKVR
88


DIM-X-PEP
FQWQRNIRKVR X FQWQRNIRKVR
89


R-DIM-PEP
FQWQRNIRKVR   RVKRINRQWQF
90


R-DIM-X-PEP
FQWQRNIRKVR X RVKRINRQWQF
91


 


PEP-313
FWQRNIRIRR
4


DIM-PEP-313
FWQRNIRIRR   FWQRNIRIRR
92


DIM-X-PEP-313
FWQRNIRIRR X FWQRNIRIRR
93


R-DIM-PEP-313
FWQRNIRIRR   RRIRINRQWF
94


R-DIM-X-PEP-313
FWQRNIRIRR X RRIRINRQWF
95


 


PEP-314
RRIRINRQWF
80


DIM-PEP-314
RRIRINRQWF   RRIRINRQWF
96


DIM-X-PEP-314
RRIRINRQWF X RRIRINRQWF
97


R-DIM-PEP-314
RRIRINRQWF   FWQRNIRIRR
98


R-DIM-X-PEP-314
RRIRINRQWF X FWQRNIRIRR
99


 


PEP-315
FWQRNIRKWR
17


DIM-PEP-315
FWQRNIRKWR   FWQRNIRKWR
100


DIM-X-PEP-315
FWQRNIRKWR X FWQRNIRKWR
101


R-DIM-PEP-315
FWQRNIRKWR   RWKRINRQWF
102


R-DIM-X-PEP-315
FWQRNIRKWR X RWKRINRQWF
103


 


PEP-316
RWKRINRQWF
66


DIM-PEP-316
RWKRINRQWF   RWKRINRQWF
104


DIM-X-PEP-316
RWKRINRQWF X RWKRINRQWF
105


R-DIM-PEP-316
RWKRINRQWF   FWQRNIRKWR
106


R-DIM-X-PEP-316
RWKRINRQWF X FWQRNIRKWR
107


 


PEP-317
FWQRRIRKWR
24


DIM-PEP-317
FWQRRIRKWR   FWQRRIRKWR
108


DIM-X-PEP-317
FWQRRIRKWR X FWQRRIRKWR
109


R-DIM-PEP-317
FWQRRIRKWR   RWKRIRRQWF
110


R-DIM-X-PEP-317
FWQRRIRKWR X RWKRIRRQWF
111


 


PEP-318
FWQRRIRRWRR
112


DIM-PEP-318
FWQRRIRRWRR   FWQRRIRRWRR
113


DIM-X-PEP-318
FWQRRIRRWRR X FWQRRIRRWRR
114


R-DIM-PEP-318
FWQRRIRRWRR   RRWRRIRRQWF
115


R-DIM-X-PEP-318
FWQRRIRRWRR X RRWRRIRRQWF
116


 


PEP-319
PFWQRNIRKWR
64


DIM-PEP-319
PFWQRNIRKWR   PFWQRNIRKWR
117


DIM-X-PEP-319
PFWQRNIRKWR X PFWQRNIRKWR
118


R-DIM-PEP-319
PFWQRNIRKWR   RWKRINRQWFP
119


R-DIM-X-PEP-319
PFWQRNIRKWR X RWKRINRQWFP
120


 


PEP-320
FWRNIRKWR
47


DIM-PEP-320
FWRNIRKWR   FWRNIRKWR
121


DIM-X-PEP-320
FWRNIRKWR X FWRNIRKWR
122


R-DIM-PEP-320
FWRNIRKWR   RWKRINRWF
123


R-DIM-X-PEP-320
FWRNIRKWR X RWKRINRWF
124


 


PEP-322
PFWRIRIRR
51


DIM-PEP-322
PFWRIRIRR   PFWRIRIRR
125


DIM-X-PEP-322
PFWRIRIRR X PFWRIRIRR
126


R-DIM-PEP-322
PFWRIRIRR   RRIRIRWFP
127


R-DIM-X-PEP-322
PFWRIRIRR X RRIRIRWFP
128


 


PEP-215
FWRIRIRR
75


DIM-PEP-215
FWRIRIRR   FWRIRIRR
129


DIM-X-PEP-215
FWRIRIRR X FWRIRIRR
130


R-DIM-PEP-215
FWRIRIRR   RRIRIRWF
131


R-DIM-X-PEP-215
FWRIRIRR X RRIRIRWF
132


 


PEP-227
FWRRFWRR
43


DIM-PEP-227
FWRRFWRR   FWRRFWRR
133


DIM-X-PEP-227
FWRRFWRR X FWRRFWRR
134


R-DIM-PEP-227
FWRRFWRR   RRWFRRWF
135


R-DIM-X-PEP-227
FWRRFWRR X RRWFRRWF
136


 


PEP-323
PFWRIRIRRD
59


DIM-PEP-323
PFWRIRIRRD   PFWRIRIRRD
137


DIM-X-PEP-323
PFWRIRIRRD X PFWRIRIRRD
138


R-DIM-PEP-323
PFWRIRIRRD   DRRIRIRWFP
139


R-DIM-X-PEP-323
PFWRIRIRRD X DRRIRIRWFP
140


 


PEP-324
PFFWRIRIRR
60


DIM-PEP-324
PFFWRIRIRR   PFFWRIRIRR
141


DIM-X-PEP-324
PFFWRIRIRR X PFFWRIRIRR
142


R-DIM-PEP-324
PFFWRIRIRR   RRIRIRWFFP
143


R-DIM-X-PEP-324
PFFWRIRIRR X RRIRIRWFFP
144


 


PEP-325
PFWRQRIRR
52


DIM-PEP-325
PFWRQRIRR   PFWRQRIRR
145


DIM-X-PEP-325
PFWRQRIRR X PFWRQRIRR
146


R-DIM-PEP-325
PFWRQRIRR   RRIRQRWFP
147


R-DIM-X-PEP-325
PFWRQRIRR X RRIRQRWFP
148


 


PEP-326
PFWRRQIRR
81


DIM-PEP-326
PFWRRQIRR   PFWRRQIRR
149


DIM-X-PEP-326
PFWRRQIRR X PFWRRQIRR
150


R-DIM-PEP-326
PFWRRQIRR   RRIQRRWFP
151


R-DIM-X-PEP-326
PFWRRQIRR X RRIQRRWFP
152


 


PEP-327
PFWRARIRR
53


DIM-PEP-327
PFWRARIRR   PFWRARIRR
153


DIM-X-PEP-327
PFWRARIRR X PFWRARIRR
154


R-DIM-PEP-327
PFWRARIRR   RRIRARWFP
155


R-DIM-X-PEP-327
PFWRARIRR X RRIRARWFP
156


 


PEP-328
PFWRKRIRR
54


DIM-PEP-328
PFWRKRIRR   PFWRKRIRR
157


DIM-X-PEP-328
PFWRKRIRR X PFWRKRIRR
158


R-DIM-PEP-328
PFWRKRIRR   RRIRKRWFP
159


R-DIM-X-PEP-328
PFWRKRIRR X RRIRKRWFP
160


 


PEP-329
PFWRKRLRR
55


DIM-PEP-329
PFWRKRLRR   PFWRKRLRR
161


DIM-X-PEP-329
PFWRKRLRR X PFWRKRLRR
162


R-DIM-PEP-329
PFWRKRLRR   RRLRKRWFP
163


R-DIM-X-PEP-329
PFWRKRLRR X RRLRKRWFP
164


 


PEP-330
PFWRKKLKR
82


DIM-PEP-330
PFWRKKLKR   PFWRKKLKR
165


DIM-X-PEP-330
PFWRKKLKR X PFWRKKLKR
166


R-DIM-PEP-330
PFWRKKLKR   RKLKKRWFP
167


R-DIM-X-PEP-330
PFWRKKLKR X RKLKKRWFP
168


 


PEP-331
PFWRKRWRR
56


DIM-PEP-331
PFWRKRWRR   PFWRKRWRR
169


DIM-X-PEP-331
PFWRKRWRR X PFWRKRWRR
170


R-DIM-PEP-331
PFWRKRWRR   RRWRKRWFP
171


R-DIM-X-PEP-331
PFWRKRWRR X RRWRKRWFP
172


 


PEP-332
PFWRRRIRR
57


DIM-PEP-332
PFWRRRIRR   PFWRRRIRR
173


DIM-X-PEP-332
PFWRRRIRR X PFWRRRIRR
174


R-DIM-PEP-332
PFWRRRIRR   RRIRRRWFP
175


R-DIM-X-PEP-332
PFWRRRIRR X RRIRRRWFP
176


 


PEP-333
PFWRRRWRR
58


DIM-PEP-333
PFWRRRWRR   PFWRRRWRR
177


DIM-X-PEP-333
PFWRRRWRR X PFWRRRWRR
178


R-DIM-PEP-333
PFWRRRWRR   RRWRRRWFP
179


R-DIM-X-PE P-333
PFWRRRWRR X RRWRRRWFP
180


 


PEP-334
PWRIRIRR
61


DIM-PEP-334
PWRIRIRR   PWRIRIRR
181


DIM-X-PEP-334
PWRIRIRR X PWRIRIRR
182


R-DIM-PEP-334
PWRIRIRR   RRIRIRWP
183


R-DIM-X-PEP-334
PWRIRIRR X RRIRIRWP
184


 


PEP-335
PWRRIRR
83


DIM-PEP-335
PWRRIRR   PWRRIRR
185


DIM-X-PEP-335
PWRRIRR X PWRRIRR
186


R-DIM-PEP-335
PWRRIRR   RRIRRWP
187


R-DIM-X-PEP-335
PWRRIRR X RRIRRWP
188


 


PEP-336
PWRRKIRR
84


DIM-PEP-336
PWRRKIRR   PWRRKIRR
189


DIM-X-PEP-336
PWRRKIRR X PWRRKIRR
190


R-DIM-PEP-336
PWRRKIRR   RRIKRRWP
191


R-DIM-X-PEP-336
PWRRKIRR X RRIKRRWP
192


 


PEP-337
PFWRRRIRIRR
193


DIM-PEP-337
PFWRRRIRIRR   PFWRRRIRIRR
194


DIM-X-PEP-337
PFWRRRIRIRR X PFWRRRIRIRR
195


R-DIM-PEP-337
PFWRRRIRIRR   RRIRIRRRWFP
196


R-DIM-X-PEP-337
RRIRIRRRWFP X RRIRIRRRWFP
197


 


PEP-338
RRWFFWRR
198


DIM-PEP-338
RRWFFWRR   RRWFFWRR
199


DIM-X-PEP-338
RRWFFWRR X RRWFFWRR
200


R-DIM-PEP-338
RRWFFWRR   RRWFFWRR
199


R-DIM-X-PEP-338
RRWFFWRR X RRWFFWRR
200


 


PEP-339
RRWFWRR
86


DIM-PEP-339
RRWFWRR   RRWFWRR
201


DIM-X-PEP-339
RRWFWRR X RRWFWRR
202


R-DIM-PEP-339
RRWFWRR   RRWFWRR
201


R-DIM-X-PEP-339
RRWFWRR X RRWFWRR
202









Preparation of Liposomes
Appropriate amounts of respective phospholipid stock solution were dried under a stream of nitrogen and stored in vacuum overnight to completely remove organic solvents. The dry lipid film was then dispersed in phosphate buffered saline (PBS, 20 mM NaPi, 130 mM NaCl, pH 7.4) and hydrated at a temperature well above the gel to fluid phase transition of the respective phospholipid under intermittent vigorous vortex-mixing. The lipid concentration was 0.1 weight % for calorimetric experiments. Hydration was carried out in presence or absence of peptides at a lipid to peptide ratio of 25:1 and 12.5:1 using a protocol described for POPS (Jimenez-Monreal, A. M. et al. Biochim Biophys Acta 1373(1998), 209-219), DPPS (Jing, W. et al. J Peptide Sci 11(2005), 735-743) and DPPC (Sevcsik, E. et al. Biochim Biophys Acta 1768 (2007) 2586-2596). The fully hydrated samples were stored at room temperature until measurement.
Differential Scanning Calorimetry (DSC)
DSC experiments were performed with a differential scanning calorimeter (VP-DSC) from MicroCal, Inc. (USA). Heating scans were performed at a scan rate of 30° C./h (pre-scan thermostating 30 min) with a final temperature of approximately 10° C. above the main transition temperature (Tm) and cooling scans at the same scan rate (pre-scan thermostating 1 min) with a final temperature approximately 20° C. below Tm. The heating/cooling cycle was performed three times. Enthalpies were calculated by integration of the peak areas after normalization to phospholipid concentration and baseline adjustment using the MicroCal Origin software (VP-DSC version). The phase transition temperature was defined as the temperature at the peak maximum (McElhaney, R. N. Chem Phys Lipids 30(1982), 229-259).
Circular Dichroism Spectroscopy
Measurements were performed on a Jasco J 715 Spectropolar-imeter (Jasco, Germany) at room temperature using quartz cuvettes with an optical path length of 0.02 cm. The CD spectra were measured between 260 nm and 180 nm with a 0.2 nm step resolution. To improve accuracy 5 scans were averaged. Peptides were dissolved in 10 mM Hepes (pH 7.4) to a final concentration of 100 μM. Spectra were measured in the absence and presence of 1 mM sodium dodecyl sulfate (SDS) and 1 mM dodecylphosphocholine (DPC) mimicking cancer and healthy mammalian membranes, respectively. The respective peptide to surfactant molar ratios were 1:25 and 1:100. Background signals were abstracted after measurements. Percentage secondary structure calculations were done using Dichroweb, CDSSR Convolution Program using reference set 4 (Whitmore, L. and Wallace, B. A. Biopolymers 89(2008), 392-400 and Nucleic Acids Res. 32 (2004), W668-W673.
Fluorescence Spectroscopy
Fluorescence spectroscopy experiments were performed using a SPEX Fluoro Max-3 spectrofluorimeter (Jobin-Yvon, France) and spectra were analyzed with Datamax software.
ANTS/DPX Leakage
Leakage of aqueous contents from liposomes was determined using the 8-aminonaphthalene-1,3,6-trisulfonic acid/p-xylene-bis-pyridinium bromide (ANTS/DPX) assay. Lipid films were hydrated with 12.5 mM ANTS, 45 mM DPX, 68 mM NaCl, 10 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) at pH 7.4 following a standard procedure.
Subsequently, the dispersions were extruded 20 times through a polycarbonate filter of 0.1 μm pore size to obtain LUVs. Unilamellarity and size were tested by X-ray and dynamic light scattering, respectively. The ANTS/DPX encapsulating vesicles were separated from free ANTS/DPX by exclusion chromatography using a column filled with Sephadex G-75 (Amersham Biosciences) fine gel swollen in an iso-smotic buffer (10 mM HEPES, 140 mM NaCl, pH 7.4). The void volume fractions were collected and the phospholipid concentration was determined by phosphate analysis (Broekhuyse, R. M. Biochim. Biophys. Acta 152(2005), 307-315; Tao, T. and Cho, J. Biochemistry 18(1979), 2759-2765).
The fluorescence measurements were performed in 2 mL of the isosmotic buffer in a quartz cuvette at room temperature. Aliquots of LUVs were diluted with the iso-osmotic buffer to a final lipid concentration of 50 μM. Fluorescence spectra were obtained at 37° C. using an excitation wavelength of 360 nm and an emission wavelength of 530 nm and a slit width of 5 nm for both excitation and emission monochromators. Fluorescence emission was recorded as a function of time before and after the addition of incremental amounts of peptide. The fluorescence increase due to leakage and subsequent dilution of quenched dye was measured after addition of peptides. Peptides were added to final concentrations of 2, 4 and 8 μM, corresponding to peptide to lipid molar ratios of 1:25, 1:12.5 and 1:6.25, respectively.
Data are presented in terms of fluorescence intensity (IF):




  
    I
    F
  
  =
  
    
      F
      -
      
        F
        0
      
    
    
      
        F
        max
      
      -
      
        F
        0
      
    
  




F is the measured fluorescence, F0 the initial fluorescence without peptide and Fmax the fluorescence corresponding to 100% leakage gained by addition of 1% Triton X-100.
Tryptophan Quenching
Tryptophan fluorescence spectra were obtained at room temperature using an excitation wavelength of 282 nm and a slit width of 5 nm for excitation and emission monochromators. Quenching of Tryptophan was carried out in the presence and absence of phospholipid liposomes (lipid to peptide ratio 25:1) using 0.1, 0.4 and 0.7 M acrylamide. The data were analyzed according to the Stern-Volmer equation:

F0/F=1+KSV[Q]

where F0 and F represent the fluorescence emission intensities in the absence and presence of the quencher molecule (Q) and KSV is the Stern-Volmer quenching constant, which is a quantitative measure for the accessibility of tryptophan to acrylamide (Tao, T. and Cho, J. Biochemistry 18(1979), 2759-2765).

Cell Lines and Culture
The primary human melanoma cell line SBcl2 and the metastatic melanoma WM164 were maintained in RPMI (Sigma) supplemented with 2% FBS, 2% L-glutamine and 1% Pen/Strep. Glioblastoma (U87-mg) purchased from CLS (Cell Line Service Heidelberg, Germany) and Rhabdomyosarcoma cell lines (TE671) purchased from ECAAC (Health Protection Agency Culture Collections Salisbury, UK) are cultured in Dulbecco's Modified Eagle Medium (DMEM) with addition of 2 mM Glutamine, 10% FBS (fetal bovine serum). Melanoma cell line A375 CLS (Cell Line Service Heidelberg, Germany) was cultured in Dulbecco's Modified Eagle Medium (DMEM)(PAA) with addition of 2 mM Glutamine and 10% FBS (fetal bovine serum. Human melanocytes used as healthy control cells: were isolated from the foreskin). The foreskin was cut into small pieces and incubated with 0.3% trypsin (PAA) overnight at 4° C. and for one hour at 37° C. Epidermis was separated. Cells were mechanically removed from the cell layer and centrifuged at 300 g for 3 min. The pellet was resuspended in melanocyte growth media (Biomedica). Melanocytes were further cultured in human melanocytes growth medium (PromoCell GmbH). Normal human dermal fibroblasts (NHDF) purchased from (PromoCell GmbH) were cultured in fibroblast growth medium 2 (PromoCell GmbH). All cells were kept in a 5% CO2 atmosphere at 37° C. At 90% confluency cell-culture flasks were passaged with accutase. All cell cultures were periodically checked for mycoplasma.
PI-Uptake Assay
For detection of PI-uptake by fluorescence spectroscopy experiments were performed according to the following protocol.
Cells were collected, resuspended in media and diluted to a concentration of 106 cells/ml. Aliquots of 105 cells were incubated with peptides for up to 8 hours at 37° C. and 5% CO2. PI was added and cells were again incubated for 5 min at room temperature in the dark. Excitation and emission wavelengths were 536 nm and 617 nm, respectively.
Cytotoxicity was calculated from the percentage of PI positive cells in media alone (P0) and in the presence of peptide (PX). Triton-X was used to determine 100% of PI positive cells (P100).




  
    %
    ⁢
    
      
    
    ⁢
    PI
    ⁢
    
      -
    
    ⁢
    uptake
  
  =
  
    
      100
      *
      
        (
        
          
            P
            X
          
          -
          
            P
            0
          
        
        )
      
    
    
      (
      
        
          P
          100
        
        -
        
          P
          0
        
      
      )
    
  




For detection of PI-uptake by fluorescence microscopy experiments were performed on a Leica DMI6000 B with IMC in connection with a Leica DFC360 FX camera and AF 6000 software.
Cells (1-5×104) were seeded on Ibidi p-Slide 8 wells and grown in 300 μl media for 2-3 days to a confluent layer. Propidium iodide (PI, 2 μl of 50 μg/ml in PBS) was added to the well and cell status was checked after 5 min of incubation in the dark at room temperature. Then, peptides were added to the desired concentration and peptide effect was followed immediately. Pictures were taken every 5 or 15 min for up to 8 h from the same section of cells. Excitation and emission wavelength were as follows: PI excitation, 535 nm and emission, 617 nm.
MTS Viability Assay
Cell proliferation was measured by using a CellTiter 96 AQ nonradioactive cell proliferation assay (Promega). Cells were plated in 96-well plates and grown until confluence. Peptides were added to a final concentration of 5-100 μM. After incubation for 24 h at 37° C. (5% CO2) MTS [3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]-phenozine methosulfate solution (20 μl/well) was added and cells were again incubated for 2 h at 37° C. (5% CO2). The MIS compound is bioreduced by cells into a colored formazan product that is soluble in tissue culture medium. The quantity of the formazan product as measured by the amount of 490 nm absorbance is directly proportional to the number of living cells in culture. Data are calculated as a percentage of the control (untreated) samples and represent the average of three wells in one experiment which was repeated three times per cell line.
Spectrofluorimetric analysis of caspase-3/7 activity 5×105 cells/ml were seeded into 96-well plate and grown overnight at 37° C. and 5% CO2. Cells were incubated with different concentrations of peptide for 4 hours. Apo-ONE® caspase-3/7 reagent was added in a 1:1 volume ratio and cells were incubated for 4 hours. Cells were then analyzed by fluorescence spectroscopy (GloMax®-Multi+ Microplate Multimode Reader with InstincTM). Untreated cells were used as negative control. Analysis was performed with Apo-ONE® Homogeneous Caspase-3/7 Assay (see FIG. 7).
Hemolysis
The hemolytic activity towards human red blood cells (RBCs), which were obtained from heparinized human blood, was determined by the release of hemoglobin following one hour incubation at 37° C. in MHNA (Mueller Hinton cation Non Adjusted). Percentage of hemolysis of RBCs was calculated using 1% Triton as 100% lysis and PBS as 0% lysis, peptide concentration was 500 μg/ml.
Results
In the present examples, toxicity, respectively selectivity of the peptides of the present invention against melanoma, rhabdomyosarcoma and glioblastoma cell lines that expose the negatively charged lipid phosphatidylserine on the outside was examined. Selective peptides are not toxic against normal non-tumor cells as melanocytes and fibroblasts or red blood cells in the same concentration range.
Selective and active peptides can be (retro-) isolated peptides (combination of the peptide moieties) of the present invention with or without linkers exhibiting defined secondary structures (as defined above) and show cancer selective activity in vitro and in cancer model systems.
Exemplarily 4 peptides were chosen to represent the observed effects. The results present data gained on peptide PEP-322 and PEP-318, representative for peptide moieties but non-active peptides, R-DIM-P-PEP-322 representative for cancer active and specific peptides and DIM-PEP-318 representative for cancer and non-cancer active, non-selective peptides (see Table 2).
Besides R-DIM-P-PEP-322 the peptides, DIM-PEP-322, R-DIM-PEP-322, R-DIM-PEP-316, R-DIM-PEP-323, DIM-PEP-324, R-DIM-P-PEP-324, R-DIM-P-PEP-332, R-DIM-P-PEP-334 were shown to be selective for cancer cells.







TABLE 2 







Overview of peptide sequences, net charge and


hydropho-bicity of the peptides examined












Net
ΔGwifa



sequence
charge
[kcal/mol]













PEP-322
PFWRIRIRR
+5
n.d.



(SEQ ID NO: 51)




 


R-DIM-P-
PFWRIRIRRPRRIRIRWFP
+9
−2.00/−2.60


PEP-322
(SEQ ID NO: 128)




 


PEP-318
FWQRRIRRWRR
+7
n.d.



(SEQ ID NO: 112)




 


DIM-PEP-
FWQRRIRRWRRFWQRRIRRWRR
+13
−8.17/−7.91


318
(SEQ ID NO: 113)





aPeptide hydrophobicity expressed as transfer free energy of peptides from water to bilayer interface (ΔGwif) calculated from the whole-residue hydrophobicity scale taking into account the contribution of the C-terminal amide (Wimley et al., Biochemistry 35 (1995), 5109-5124) and the % helix gained from the respective CD data Since for the short peptides PEP-322 and -318 the CD measurements are not accurate enough the ΔGwif was only calculated for the isolated peptides.






Peptide Structure—Activity and Selectivity
In Silico—Secondary Structure Prediction
“Isolated peptides” of several PEP peptides were first analyzed by simulation of the secondary structure. The secondary structure of putative membrane active isolated peptides were predicted by the online program PEP-FOLD: http://bioserv.rpbs.univ-paris-diderot.fr/PEP-FOLD/ (Maupetit, J et al. Nucleic Acids Res. 37 (2009), W498-W503). From this analysis several peptides were selected for synthesis and activity studies according to their high proportion of amphipathic β-sheet or α-helical structure.
The non-active peptide moieties partially turned out to be too short for assembly of a defined secondary structure. Interestingly the cancer specific peptides (DIM-, R-DIM-, R-DIM-P-PEP-322, DIM-PEP-324 and R-DIM-PEP-316) formed 2 β-strands or 2 α-helices (R-DIM-PEP-316) with a turn in the middle and distribution of cationic and hydrophobic regions. For the active but non-selective peptide DIM-PEP-318 a linear amphipathic α-helix without a loop was predicted (see Table 3, FIG. 13). For human Lactoferricin (hLFcin) (37-61) containing a disulfide bridge via Cys3 and Cys20 a structure with a helical and an extended part linked via a loop was predicted which is in agreement with the structure reported for hLFcin in a membrane mimetic solvent (Gifford, J L et al. Cell. Mol. Life Sci. 62 (2005), 2588-2598).







TABLE 3







List of secondary structure prediction and cancer specificity for isolated


peptides (-retro); list includes all sequences in isolated peptide (DIM-)


(peptide moiety sequence + peptide moiety sequence as defined herein),


isolated peptide retro (R-DIM-) (peptide moiety sequence + peptide moiety


retro sequence), isolated peptide with linker X (DIM-X-) (peptide moiety


sequence + X + peptide moiety sequence), isolated peptide retro with


linker X (R-DIM-X-) (peptide moiety sequence + X + peptide moiety


retro sequence). H = helix, T = turn, β = β-strand. For positive


or negative peptide specificity for tumor over non tumor cells in the case


of studied peptides the respective -fold specificity for melanoma (SBcl2


or A375) over non tumor skin cells (melanocytes or normal human dermal


fibroblasts (NHDF-c)) at 20 μM peptide concentration after 8 hours


incubation is listed (derived by PI uptake studies).









peptide




moieties
Secondary Structure Prediction
Cancer












(parents)
DIM-
DIM-X-
R-DIM-
R-DIM-X-
Specificity





PEP
H
2H T
H
2H T



PEP-313
H T
2β T
H
H T β



PEP-314
2H
2β T
3β 2T
2β T



PEP-315
H
2H T
H
2H T



PEP-316
H T
2H T
2H T
2H T
Yes (15fold) (R-DIM-)


PEP-317
H
2H T
H
2H T



PEP-318
H
2H T
H
2H T
No (<1fold) (DIM-)


PEP-319
2H T
2H T
H
2H T



PEP-320
H
2H T
2H T
2H T



PEP-322
2β T
2β T
2β T
2β T
yes (50fold)







(DIM-), 20fold







(R-DIM-), >100fold)







(R-DIM-X-)


PEP-215
2β T
2β T
2β T
2β T



PEP-227
2H
2H T
H
2H T



PEP-323
2 β T
2β T
2β T
4β T



PEP-324
2β T
2β T
2β T
2β T
yes (7fold) (DIM-),







(15fold) (R-DIM-X-)


PEP-325
2H T
2H T
H
2H T



PEP-326
2H T
2H T
H
2H T



PEP-327
2H T
2H T
H
2H T



PEP-328
2H
2H T
H
2H T



PEP-329
2H
2H T
H
2H T



PEP-330
2H
2H T
H
2H T



PEP-331
2H
2H T
H
2H T



PEP-332
2H
2H T
H
2H T
(5fold) (R-DIM-X-)


PEP-333
2H
2H T
H
2 H T



PEP-334
2β T
2β T
2β T
2β T
(15fold) (R-DIM-X-)


PEP-335
2H
2H T
H
2H T



PEP-336
H
2H T
H
2H T



PEP-337
2H T
2H T
H
2H T
weak







(2fold) (R-DIM-)


PEP-338
2β T
2β T
=DIM-
=DIM-X-



PEP-339
2β T
2β T
=DIM-
=DIM-X-









Model Studies
Circular Dichroism Spectroscopy—Secondary Structure Vs. Activity and Selectivity
Strikingly the selective peptide R-DIM-P-PEP-322 (FIG. 1B) exhibits a significant increase of β-sheet conformation in the presence of the cancer mimic SDS, α-helical content is even further decreased. Moreover the structure of the peptide in the presence of the healthy mimic DPC is the same as in solution, giving further hint for the cancer selective toxicity of these peptides (see also FIG. 2).
Percentage secondary structure calculations were done using Dichroweb, CDSSR Convolution Program using reference set 4 (Whitmore et al., Nucleic Acids Res. 32 (2004), W668-W67; Whitmore et al., Biopolymers 89 (2008), 392-400). The α-helical content is shown in dark gray at the bottom; β-turns in light grey; turns in middle grey; random coil structures in dark grey at the top.
FIG. 2 now presents the results of circular dichroism spectroscopy of the non-specific peptide DIM-PEP-318 in contrast to the specific peptide R-DIM-PEP-322. In contrast to the PEP-322 peptide group, DIM-PEP-318 possesses a higher α-helical content in the presence of the cancer mimic SDS as well as in the presence of the non-cancer mimic DPC. DIM-PEP-318 adopts up to 75% α-helical structure without discrimination between cancer and non-cancer cell mimic.
In Vitro Studies—Membrane Permeabilization
PI-Uptake—Increase of Activity by Sequence Doubling
Cytotoxic activity of the peptides towards melanoma cells of primary (SBcl-2) and metastatic lesions (WM164) and differentiated non-tumorigenic melanocytes was determined by measurement of PI-uptake, which only occurs when integrity of the cell membrane is lost. Cells were incubated in media containing serum for 8 h in the presence of peptides. Peptide concentrations were varied from 10 to 80 μM. FIG. 3 illustrates that the peptide moiety PEP-322 is only minor active against the melanoma cell line SBcl-2 with less than 5% killing at a peptide concentration of 80 μM, as well as against melanocytes with a moderate two-fold selectivity for WM164 cells at 20 μM peptide concentration (FIG. 3C). The isolated peptide by combination of the peptide moiety with its retro sequence and a Pro linker R-DIM-P-PEP-322 shows strongly increased activity against SBcl-2 compared to the its peptide moiety with very high specificity for the melanoma cell line. Already at a peptide concentrations of 20 μM, R-DIM-P-PEP-322 yields more than 80% PI positive SBcl-2 cells, while only less than 1% of differentiated non-tumorigenic melanocytes are killed (FIG. 3B), exhibiting a specificity more than 100-fold for cancer cells (see FIG. 3C). The second melanoma cell line, WM164, tested at 20 μM R-DIM-P-PEP-322 peptide concentration is also highly sensitive for the peptide.
PI-Uptake—Specificity and Time Dependence of Killing-Correlation of Specificity with Structure
Cytotoxic activity of the peptides towards melanoma cells of primary (SBcl-2) and metastatic lesions (WM164), a rhabdomyosarcoma cell line (TE671) and their healthy counterparts differentiated non-tumorigenic melanocytes and normal human dermal fibroblasts (NHDF) was determined by measurement of PI-uptake, which indicates a loss of cell membrane integrity (FIGS. 4 and 5). Cells were incubated in media containing serum for up to 8 h in the presence of peptides at 20 μM peptide concentration.
Both peptide moieties PEP-322 as well as PEP-318 are only minor active against cancer cells (<30%, see FIG. 4A-C). However, PEP-318 is even slightly active against non-cancer cells, killing up to  ̃30% of NHDF. Interestingly, the combination of the peptide moiety PEP-322 and its retro sequence in form of the isolated peptide R-DIM-P-PEP-322 shows high cancer toxicity (up to 80%) but with negligible non-cancer toxicity (see FIG. 4D-E). In contrast to DIM-PEP-318, R-DIM-P-PEP-322 kills quite slowly reaching its highest activity not before 4-8 h. The second peptide moiety combination, namely DIM-PEP-318, possesses the highest and fastest anticancer activity with up to 90% killing within minutes (FIG. 4A-C). However, DIM-PEP-318 reveals to be quite unspecific since it is also highly active against differentiated non-tumorigenic melanocytes as well as against normal human dermal fibroblasts (FIG. 4D-E). Cytotoxic activity of the peptides after 8 h of incubation is given in FIG. 11. As can be seen in FIG. 12 all peptides with structure predictions of an α-helix without a loop exhibit toxicity against non-tumor and tumor cell lines. The parent peptide hLFcin (37-61) comprising contrary to the peptides claimed here within a disulfide bond via two cysteins (Cys3 and Cys20) shows only weak activity against the tested melanoma cell line A375 and glioblastoma cell line U87 mg and no toxicity against normal human dermal fibroblasts NHDF-c.
As shown in FIGS. 14 and 15 the isolated peptides show high cytotoxicity against the cell lines of malignant melanoma (A375) and glioblastoma (U87 mg) after 8 hours of incubation at 20 μM peptide concentration. The cytotoxicity is very much improved compared to that exhibited by the parent peptide h LFcin. These two cancer types exhibit so far very poor treatability and bad prognosis.
Cell Viability—MTS Cell Proliferation
To determine long-time toxicity of peptides, a MTS cell proliferation assay was used to elucidate cell viability upon 24 h incubation with variant peptide concentration and human melanoma cell lines SBcl-2 and WM164 and non-differentiated human skin fibroblast cell line NHDF. As shown in FIG. 6A PEP-322 affects cancer cells and non-cancer cells only marginally even at higher peptide concentrations of 100 μM sustaining more than 70% cell viability (IC50 WM164 and NHDF>100 μM) and 40% (IC50 SBcl-2 90). Combination of peptide moieties could highly improve anticancer activity. R-DIM-P-PEP-322 exhibits a decreased IC50 value of 8 μM and 15 μM for melanoma cell line SBcl-2 and melanoma metastasis WM164, respectively (FIG. 6B) compared to an IC50 value of 80 μM for the non-cancer cell line, yielding 8-5-fold selectivity for cancer cells. DIM-PEP-318 shows also high activity against cancer cells resulting in an IC50<10 μM (FIG. 6C). As indicated already by PI uptake studies this peptide is as toxic for non-cancer cells, revealed by a very low IC50 of 10 μM for NHDF, as well. Results of MTS and PI assay with cancer cells correlated well. An overview of IC50 values is given in Table 6.







TABLE 6







Comparison of IC50 values determined through PI-uptake


(8 h) and MTS cell viability assay (24 h).










SBcl-2 (PI/MTS)
Fibroblasts (PI/MTS)














PEP-322
>80 
μM/90 μM
>>80 
μM/>100 μM


R-DIM-P-PEP-322
8 
μM/8 μM
>>80 
μM/80 μM









PEP-318
n.d./n.d. 
n.d./n.d.











DIM-PEP-318
<20 
μM/6 μM
<20 
μM/10 μM









Hemolytic Activity Against Red Blood Cells—Specificity
Hemolytic activity of peptides against red blood cells was tested at 500 μg/ml peptide and 2.5% red blood cell concentration. It was very surprising that DIM-PEP-318 was not hemolytic, considering the high toxicity towards melanocytes and fibroblasts.







TABLE 7







Hemolytic activity of peptides against human red blood cells












% lysis of 2.5%





RBCsa
IC50 [μg/ml]







PEP-322
2b
>500b



R-DIM-P-PEP-322
0.84+ 0.63
>500



PEP-318
n.d.
n.d.



DIM-PEP-318
2.87+ 0.57
>500







aPercentage of hemolysis of human red blood cells (RBCS) was calculated following one hour incubation at 37° C. in PBS using 1% Triton X-100 as 100% lysis and PBS as 0% lysis, peptide concentration was 500 μg/ml.



bfrom Zweytick et al., J. Biol. Chem. 286 (2011),.



n.d. not determined






Caspase-3 Cleavage-Apoptosis or Necrosis
To clearly differentiate between necrotic and apoptotic killing a caspase-3/7 activity assay was used to detect emergence of apoptosis (FIG. 7). R-DIM-P-PEP-322 showed apoptosis indicated by a strong increase of caspase-3/7 activity from the time-point of 4 hours of incubation of melanoma cells SBcl-2 with 10-20 μM peptide, indicated by a 200-fold increase of green fluorescence. The non-specific peptide DIM-PEP-318 showed much lower caspase-3/7 activity, indicating a non-apoptotic killing mechanism like necrosis shown by a strong PI-uptake by the peptide (FIG. 8-11).
Additionally, apoptotic like blebbing of the cell membrane is observed during incubation of the rhabdomyosarcoma cell line TE671 in the presence of the peptide.







TABLE 8







Correlation of activity exhibited by peptides PEP-322


and R-DIM-P-PEP-322 in model and in vitro studies.











R-DIM-P-


Peptide
PEP-322
PEP-322





amino acid sequence
PFWRIRIRR-(NH2)
-PRRIRIRWFP-NH2


net charge
+5
+9







cancer mimic/healthy mimic









bilayer perturbation-
+/−
+++/−


Differential Scanning




Calorimetry




permeability-
+/−
+++/−


ANTS/DPX leakage




bilayer affinity-
++/−
++/−


quenching




structure-CD
> β-sheet/as in
> β-sheet/as in 



solution
solution







cancer cells/healthy cells









toxicity-PI uptake, 
−/−
+++/−


MTT




cancer specificity
(+)
++++
















TABLE 9







Correlation of activity exhibited by selective peptide


R-DIM-P-PEP-322 and non-selective peptide DIM-PEP-318 in model


and in vitro studies.









Peptide
R-DIM-P-PEP-322
DIM-PEP-318





amino acid sequence
PFWRIRIRR-P-
FWQRRIRRWRR-


net charge
RRIRIRWFP-NH2
FWQRRIRRWRR-NH2



+9
+13







cancer mimic/healthy mimic









bilayer perturbation-
+++/− 
+++/+++


Differential Scan-




ning Calorimetry




permeability-leak-
+++/− 
+++/+


age




bilayer affinity-
++/− 
++/+


quenching




structure-CD
> β-sheets/as in
> α-helical/> α-



solution
helical







cancer cells/healthy cells









toxicity-PI up- 
+++/−
+++/+++


take, MTT




cancer specificity
++++
−−−









Discussion
In this example the selective antitumor activity of the peptides of the present invention could be demonstrated. The peptide moieties (containing no disulfide bridge) derived of the membrane active part of hLFcin such as PEP-322 and hLFcin (37-61) (one disulfide bridge) itself exhibited only weak activity against melanoma cancer cell lines, the combination of peptide moieties in R-DIM-P-PEP-322 (comprising 2 beta-strands separated by a turn) showed highly increased activity. PI-uptake of melanoma cells upon incubation with peptide R-DIM-P-PEP-322 further demonstrates that the peptide operates via a membrane mediated way, since PI can only be taken up by cells that suffer membrane disintegration. Improved interaction of the isolated peptide with the cancer mimic PS correlated with increased activity against the melanoma cancer cell line and non-interaction with the healthy mimic PC correlated with non-toxicity against non-cancer melanocytes. The isolated peptide exhibits a high membrane destabilization emphasized by highly increased membrane permeability of PS bilayers. Besides, permeability studies show that a certain threshold concentration of the isolated peptide is needed for induction of sufficient leakage of ANTS/DPX, differentiating it from highly lytic but mostly unspecific peptides like melittin. In agreement also the effect on neutral lipids is negligible. Moreover by calorimetric studies it could be demonstrated that the effect of the isolated peptide is even much higher than that of the peptide moiety at doubled concentration, rather suggesting a structural effect than a simple mass and charge effect.
Trp localization studies of peptides showed that if a peptide is active against a certain membrane, it exhibits a significant blue shift of Trp emission wavelength upon interaction with the membrane indicating a more hydrophobic environment of Trp due to interaction with the membrane interface. In the case of the peptide moiety PEP-322 and combination of peptide moieties R-DIM-P-PEP-322 the blue shift is only detected in presence of the target lipid PS present on the surface of cancer membranes, whereas in the presence of PC no blue shift appears, going hand-in-hand with a selective toxicity against cancer cells in vitro. These findings are in line with the ability of Trp quenching, which is strongly decreased only in the presence of the target lipid PS. Non-selective peptides like DIM-PEP-318 however reveal a blue shift in the presence of both model systems.
Further structural information on the studied peptides was given by CD experiments. Again structural changes for PEP-322 and the isolated peptide appear only in the presence of the negatively charged cancer mimic (SDS). The peptide DIM-PEP-318 changes its structure in environment of both models conform to its low specificity. Only the non-selective peptide shows an increase of the α-helical content in the presence of both model systems, differently PEP-322 and R-DIM-P-PEP-322 show an increase of the β-sheet content upon presence of the cancer model SDS.
From the differences in activity displayed by the peptide moiety and the combination thereof in the isolated peptide it was however surprising that both peptides show quite similar structural characteristics in solution and model system. Considering the shortness of the moiety PEP-322, it is even questionable if a β-sheet conformation is possible. It is moreover reasonable that two moiety peptide stretches arrange on the lipid surface like a dimer, but not covalently linked. The combined moieties in the isolated peptide however are fixed in this conformation via peptide bond and will create stronger membrane perturbance and finally higher membrane permeabilization, which can explain its highly increased activity in model and cell system.
On the one hand it was demonstrated that high membrane interaction of the isolated peptides derived from the membrane active peptide PEP with anionic PS correlates with high activity against melanoma cells, on the other hand it could be shown that increasing interaction with the healthy mimic neutral PC correlates with decreased specificity indicated by increased interaction with non-cancer cell types like melanocytes or fibroblasts. This was demonstrated for the combination of two peptide moieties PEP-318, namely DIM-PEP-318 also originally derived from PEP. The short peptide moieties PEP-322 and PEP-318 are only minor active against cancer cells even if incubation time is extended to 8 h or higher peptide concentrations are used. Partially the low activity can also be due to less defined structure. In contrast, the combination of these peptide moieties in DIM-PEP-322 and DIM-PEP-318 exhibit increased anticancer activity. However the different peptides seem to operate by different mechanisms, since peptide DIM-PEP-318 reaches its maximum toxicity against cancer cells already after 15 minutes, whereas contrariwise R-DIM-PEP-322 kills much slower reaching its maximum activity not before 8 hours. Nevertheless it shows similar or even increased cancer toxicity compared to DIM-PEP-318, after long time period. The different time dependence of cell killing by the peptides indicates 2 different killing mechanism. The very fast action of DIM-PEP-318 gives strong evidence for a direct membranolytic effect causing necrosis. Prediction of the secondary structure of the peptides proposes an amphiphatic α-helix. Structural analysis through CD spectroscopy of DIM-PEP-318 also reveals induction of a mostly α-helical structure in the presence of the cancer as well as the non-cancer mimic resulting in non-selective lysis of cells. The selective peptide R-DIM-P-PEP-322 obviously acts via a different mechanism. The relatively slow action together with the observation of membrane blebbing and Caspase-3/7 activity is an indication for membrane-mediated apoptosis. For induction of apoptosis the peptide has to enter the cell specifically over probably the PS compartments on the surface and further reach another negatively charged target on the surface of cancer cell mitochondria, like cardiolipin. Successive swelling of mitochondria and release of cytochrome-C activate the caspase dependent pathway of the programmed cell death. Interestingly R-DIM-P-PEP-322 shows induction of an increase in the predominant β-sheet structure with a turn and no changes in structure in the presence of the non-cancer cell mimic. In the secondary structure prediction an arrangement of 2 β-strands with hydrophobic endings with a loop in the middle composed of cationic amino acids was predicted. According to other prediction studies the amphipathic distribution of amino acids with a loop between 2 β-strands or 2 α-helices seem to be important structural features for an active and specific peptide.

## Claims

1. A method of treating cancer comprising administering an isolated peptide selected from the group consisting of
PFWRIRIRRXRRIRIRWFP (SEQ ID NO: 128),
PWRIRIRRXRRIRIRWP (SEQ ID No. 184),
RWKRINRQWFFWQRNIRKWR (SEQ ID No. 106),
PFWRIRIRRPFWRIRIRR (SEQ ID No. 125),
PFFWRIRIRRPFFWRIRIRR (SEQ ID No. 141), and
PFWRIRIRRRRIRIRWFP (SEQ ID No. 127), wherein “X” is proline (Pro) or glycine(Gly)1-3,

wherein the cancer is melanoma, glioblastoma, or rhabdomyosarcoma.

2. The method of claim 1, wherein the peptide is SEQ ID No. 128, SEQ ID No. 184, or SEQ ID No. 106.