# Patent

## Title
Arylindenopyridines and arylindenopyrimidines and related therapeutic and prophylactic methods

## Abstract

This invention provides novel arylindenopyridines and arylindenopyrimidines of the formula:




wherein R1, R2, R3, R4, and X are as defined above, and pharmaceutical compositions comprising same, useful for treating disorders ameliorated by antagonizing adenosine A2a receptors. This invention also provides therapeutic and prophylactic methods using the instant compounds and pharmaceutical compositions.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a divisional of co-pending application Ser. No. 10/678,562 filed on Oct. 3, 2003, which is a continuation-in-part of application Ser. No. 10/259,139, filed on Sep. 27, 2002, now U.S. Pat. No. 6,903,109, which is a continuation-in-part of application Ser. No. 10/123,389, filed on Apr. 16, 2002, now U.S. Pat No. 6,958,328, all of which are incorporated herein by reference.


FIELD OF THE INVENTION
This invention relates to novel arylindenopyridines and arylindenopyrimidines and their therapeutic and prophylactic uses. Disorders treated and/or prevented using these compounds include neurodegenerative and movement disorders ameliorated by antagonizing Adenosine A2a receptors.
BACKGROUND OF THE INVENTION
Adenosine A2a Receptors
Adenosine is a purine nucleotide produced by all metabolically active cells within the body. Adenosine exerts its effects via four subtypes of cell-surface receptors (A1, A2a, A2b and A3), which belong to the G protein coupled receptor superfamily (Stiles, G. L. Journal of Biological Chemistry, 1992, 267, 6451). A1 and A3 couple to inhibitory G protein, while A2a and A2b couple to stimulatory G protein. A2a receptors are mainly found in the brain, both in neurons and glial cells (highest level in the striatum and nucleus accumbens, moderate to high level in olfactory tubercle, hypothalamus, and hippocampus etc. regions) (Rosin, D. L.; Robeva, A.; Woodard, R. L.; Guyenet, P. G.; Linden, J. Journal of Comparative Neurology ,1998, 401, 163). In peripheral tissues, A2a receptors are found in platelets, neutrophils, vascular smooth muscle and endothelium (Gessi, S.; Varani, K.; Merighi, S.; Ongini, E.; Borea, P. A. British Journal of Pharmacology, 2000, 129, 2). The striatum is the main brain region for the regulation of motor activity, particularly through its innervation from dopaminergic neurons originating in the substantia nigra. The striatum is the major target of the dopaminergic neuron degeneration in patients with Parkinson's Disease (PD). Within the striatum, A2a receptors are co-localized with dopamine D2 receptors, suggesting an important site for the integration of adenosine and dopamine signaling in the brain (Fink, J. S.; Weaver, D. R.; Rivkees, S. A.; Peterfreund, R. A.; Pollack, A. E.; Adler, E. M.; Reppert, S. M. Brain Research Molecular Brain Research, 1992, 14, 186).
Neurochemical studies have shown that activation of A2a receptors reduces the binding affinity of D2 agonist to their receptors. This D2R and A2aR receptor-receptor interaction has been demonstrated in striatal membrane preparations of rats (Ferre, S.; von Euler, G.; Johansson, B.; Fredholm, B. B.; Fuxe, K. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88, 7238) as well as in fibroblast cell lines after transfected with A2aR and D2R cDNAs (Salim, H.; Ferre, S.; Dalal, A.; Peterfreund, R. A.; Fuxe, K.; Vincent, J. D.; Lledo, P. M. Journal of Neurochemistry, 2000, 74, 432). In vivo, pharmacological blockade of A2a receptors using A2a antagonist leads to beneficial effects in dopaminergic neurotoxin MPTP(1-methyl-4-pheny-1,2,3,6-tetrahydropyridine)-induced PD in various species, including mice, rats, and monkeys (Ikeda, K.; Kurokawa, M.; Aoyama, S.; Kuwana, Y. Journal of Neurochemistry, 2002, 80, 262). Furthermore, A2a knockout mice with genetic blockade of A2a function have been found to be less sensitive to motor impairment and neurochemical changes when they were exposed to neurotoxin MPTP (Chen, J. F.; Xu, K.; Petzer, J. P.; Staal, R.; Xu, Y. H.; Beilstein, M.; Sonsalla, P. K.; Castagnoli, K.; Castagnoli, N., Jr.; Schwarzschild, M. A. Journal of Neuroscience, 2001, 21, RC143).
In humans, the adenosine receptor antagonist theophylline has been found to produce beneficial effects in PD patients (Mally, J.; Stone, T. W. Journal of the Neurological Sciences, 1995, 132, 129). Consistently, recent epidemiological study has shown that high caffeine consumption makes people less likely to develop PD (Ascherio, A.; Zhang, S. M.; Heman, M. A.; Kawachi, I.; Colditz, G. A.; Speizer, F. E.; Willett, W. C. Annals of Neurology, 2001, 50, 56). In summary, adenosine A2a receptor blockers may provide a new class of antiparkinsonian agents (Impagnatiello, F.; Bastia, E.; Ongini, E.; Monopoli, A. Emerging Therapeutic Targets, 2000, 4, 635).
SUMMARY OF THE INVENTION
This invention provides a compound having the structure of Formula I or II




    
    
        or a pharmaceutically acceptable salt thereof, wherein
        (a) R1 is selected from the group consisting of
        
            (i) —COR5, wherein R5 is selected from H, optionally substituted C1-8 straight or branched chain alkyl, optionally substituted aryl and optionally substituted arylalkyl;
            
                wherein the substituents on the alkyl, aryl and arylalkyl group are selected from C1-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, or NR7R8 wherein R7 and R8 are independently selected from the group consisting of hydrogen, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, benzyl, aryl, or heteroaryl or NR7R8 taken together form a heterocycle or heteroaryl;
            
            
            (ii) COOR5, wherein R5 is as defined above;
            (ii) cyano;
            (iii) —CONR9R10 wherein R9 and R10 are independently selected from H, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, trifluoromethyl, hydroxy, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl;
            
                wherein the alkyl, cycloalkyl, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl, aryl, heteroaryl and heterocyclyl groups may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, amino, alkoxy or arylalkyl, or R9 and R10 taken together with the nitrogen to which they are attached form a heterocycle or heteroaryl group;
            
            
            (v) optionally substituted C1-8 straight or branched chain alkyl;
            
                wherein the substituents on the alkyl, group are selected from C1-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, carboxyl, aryl, heterocyclyl, heteroaryl, sulfonyl, thiol, alkylthio, or NR7R8 wherein R7 and R8 are as defined above;
            
            
        
        
        (b) R2 is selected from the group consisting of optionally substituted 25 alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl and optionally substituted C3-7 cycloalkyl, C1-8 alkoxy, aryloxy, C1-8 alkylsulfonyl, arylsulfonyl, arylthio, C1-8 alkylthio, or —NR24R25 
        
            wherein R24 and R25 are independently selected from H, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or R24 and R25 taken together with the nitrogen form a heteroaryl or heterocyclyl group,
        
        
        (c) R3 is from one to four groups independently selected from the group consisting of:
        
            hydrogen, halo, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, C1-8 alkoxy, cyano, C1-4 carboalkoxy, trifluoromethyl, C1-8 alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, C1-8 carboxylate, aryl, heteroaryl, and heterocyclyl, —NR11R12,
            
                wherein R11 and R12 are independently selected from H, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or R10 and R11 taken together with the nitrogen form a heteroaryl or heterocyclyl group,
            
            
            —NR13COR14,
            
                wherein R13 is selected from hydrogen or alkyl and R14 is selected from hydrogen, alkyl, substituted alkyl, C1-3 alkoxyl, carboxyalkyl, aryl, arylalkyl, heteroaryl, heterocyclyl, R15R16N (CH2)p—, or R15R16NCO(CH2)p—, wherein R15 and R16 are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6, wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, substituted heteroaryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl, or R13 and R14 taken together with the carbonyl form a carbonyl containing heterocyclyl group;
            
            
        
        
        (d) R4 is selected from the group consisting of hydrogen, C1-6 straight or branched chain alkyl, benzyl
        
            wherein the alkyl and benzyl groups are optionally substituted with one or more groups selected from C3-7 cycloalkyl, C1-8 alkoxy, cyano, C1-4 carboalkoxy, trifluoromethyl, C1-8 alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, C1-8 carboxylate, amino, NR17R18, aryl and heteroaryl,
            —OR17, and —NR17R18,
            
                wherein R17 and R18 are independently selected from hydrogen, and optionally substituted C1-6 alkyl or aryl; and
            
            
        
        
        (e) X is selected from C═S, C═O; CH2, CHOH, CHOR19; or CHNR20R21 where R19, R20, and R21 are selected from optionally substituted C1-8 straight of branched chain alkyl, wherein the substituents on the alkyl group are selected from C1-8 alkoxy, hydroxy, halogen, amino, cyano, or NR22R23 wherein R22 and R23 are independently selected from the group consisting of hydrogen, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, benzyl, aryl, heteroaryl, or NR22R23 taken together from a heterocycle or heteroaryl;
        with the proviso that in a compound of Formula II when R1 is a cyano, then R2 is not phenyl.
    
    


This invention also provides a pharmaceutical composition comprising the instant compound and a pharmaceutically acceptable carrier.
This invention further provides a method of treating a subject having a condition ameliorated by antagonizing Adenosine A2a receptors, which comprises administering to the subject a therapeutically effective dose of the instant pharmaceutical composition.
This invention further provides a method of preventing a disorder ameliorated by antagonizing Adenosine A2a receptors in a subject, comprising of administering to the subject a prophylactically effective dose of the compound of claim 1 either preceding or subsequent to an event anticipated to cause a disorder ameliorated by antagonizing Adenosine A2a receptors in the subject.


DETAILED DESCRIPTION OF THE INVENTION
Compounds of Formula I are potent small molecule antagonists of the Adenosine A2a receptors that have demonstrated potency for the antagonism of Adenosine A2a, A1, and A3 receptors.
Preferred embodiments for R1 are COOR5 wherein R5 is an optionally substituted C1-8 straight or branched chain alkyl. Preferably the alkyl chain is substituted with a dialkylamino group.
Preferred embodiments for R2 are optionally substituted heteroaryl and optionally substituted aryl. Preferably, R2 is an optionally substituted furan.
Preferred substituents for R3 include hydrogen, halo, hydroxy, amino, trifluoromethyl, alkoxy, hydroxyalkyl chains, and aminoalkyl chains,
Preferred substituents for R4 include NH2 and alkylamino.
In a preferred embodiment, the compound is selected from the group of compounds shown in Tables 1 and 2 hereinafter.
More preferably, the compound is selected from the following compounds:
The compound of claim 1, formula I, wherein R4 is amino.




2-amino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one




2-amino-4-phenyl-indeno[1,2-d]pyrimidin-5-one




2-amino-4-thiophen-2-yl-indeno[1,2-d]pyrimidin-5-one




2-amino-4-(5-methyl-fu ran-2-yl)-indeno[1,2-d]pyrimidin-5-one




2,6-diamino-4-furan-2-yl-indeno[1,2-d]pyrimidin-5-one




9H-indeno[2,1-c]pyridine-4-carbonitrile, 3-amino-1-furan-2-yl-9-oxo-




9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 2-dimethylamino-ethyl ester




9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-phenyl-9-oxo-, 2-dimethylamino-ethyl ester




9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino1-furan-2-yl-9-oxo-, (2-dimethylamino-1-methyl-ethyl)-amide




9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, (2-dimethylamino-ethyl)-methyl-amide




9H-indeno[2,1-c]pyridine-4-carboxylic acid, 3-amino-1-furan-2-yl-9-oxo-, 1-methyl-pyrrolidin-2-ylmethyl ester
The instant compounds can be isolated and used as free bases. They can also be isolated and used as pharmaceutically acceptable salts. Examples of such salts include hydrobromic, hydroiodic, hydrochloric, perchloric, sulfuric, maleic, fumaric, malic, tartaric,-citric, benzoic, mandelic, methanesulfonic, hydroethanesulfonic, benzenesulfonic, oxalic, palmoic, 2-naphthalenesulfonic, p-toluenesulfonic, cyclohexanesulfamic and saccharic.
This invention also provides a pharmaceutical composition comprising the instant compound and a pharmaceutically acceptable carrier.
Pharmaceutically acceptable carriers are well known to those skilled in the art and include, but are not limited to, from about 0.01 to about 0.1 M and preferably 0.05 M phosphate buffer or 0.8% saline. Such pharmaceutically acceptable carriers can be aqueous or non-aqueous solutions, suspensions and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, ethanol, alcoholic/aqueous solutions, glycerol, emulsions or suspensions, including saline and buffered media. Oral carriers can be elixirs, syrups, capsules, tablets and the like. The typical solid carrier is an inert substance such as lactose, starch, glucose, methyl-cellulose, magnesium stearate, dicalcium phosphate, mannitol and the like. Parenteral carriers include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous carriers include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose and the like. Preservatives and other additives can also be present, such as, for example, antimicrobials, antioxidants, chelating agents, inert gases and the like. All carriers can be mixed as needed with disintegrants, diluents, granulating agents, lubricants, binders and the like using conventional techniques known in the art.
This invention further provides a method of treating a subject having a condition ameliorated by antagonizing Adenosine A2a receptors, which comprises administering to the subject a therapeutically effective dose of the instant pharmaceutical composition.
In one embodiment, the disorder is a neurodegenerative or movement disorder. Examples of disorders treatable by the instant pharmaceutical composition include, without limitation, Parkinson's Disease, Huntington's Disease, Multiple System Atrophy, Corticobasal Degeneration, Alzheimer's Disease, and Senile Dementia.
In one preferred embodiment, the disorder is Parkinson's disease.
As used herein, the term “subject” includes, without limitation, any animal or artificially modified animal having a disorder ameliorated by antagonizing adenosine A2a receptors. In a preferred embodiment, the subject is a human.
Administering the instant pharmaceutical composition can be effected or performed using any of the various methods known to those skilled in the art. The instant compounds can be administered, for example, intravenously, intramuscularly, orally and subcutaneously. In the preferred embodiment, the instant pharmaceutical composition is administered orally. Additionally, administration can comprise giving the subject a plurality of dosages over a suitable period of time. Such administration regimens can be determined according to routine methods.
As used herein, a “therapeutically effective dose” of a pharmaceutical composition is an amount sufficient to stop, reverse or reduce the progression of a disorder. A “prophylactically effective dose” of a pharmaceutical composition is an amount sufficient to prevent a disorder, i.e., eliminate, ameliorate and/or delay the disorder's onset. Methods are known in the art for determining therapeutically and prophylactically effective doses for the instant pharmaceutical composition. The effective dose for administering the pharmaceutical composition to a human, for example, can be determined mathematically from the results of animal studies.
In one embodiment, the therapeutically and/or prophylactically effective dose is a dose sufficient to deliver from about 0.001 mg/kg of body weight to about 200 mg/kg of body weight of the instant pharmaceutical composition. In another embodiment, the therapeutically and/or prophylactically effective dose is a dose sufficient to deliver from about 0.05 mg/kg of body weight to about 50 mg/kg of body weight. More specifically, in one embodiment, oral doses range from about 0.05 mg/kg to about 100 mg/kg daily. In another embodiment, oral doses range from about 0.05 mg/kg to about 50 mg/kg daily, and in a further embodiment, from about 0.05 mg/kg to about 20 mg/kg daily. In yet another embodiment, infusion doses range from about 1.0 μg/kg/min to about 10 mg/kg/min of inhibitor, admixed with a pharmaceutical carrier over a period ranging from about several minutes to about several days. In a further embodiment, for topical administration, the instant compound can be combined with a pharmaceutical carrier at a drug/carrier ratio of from about 0.001 to about 0.1.
Definitions and Nomenclature
Unless otherwise noted, under standard nomenclature used throughout this disclosure the terminal portion of the designated side chain is described first, followed by the adjacent functionality toward the point of attachment.
As used herein, the following chemical terms shall have the meanings as set forth in the following paragraphs: “independently”, when in reference to chemical substituents, shall mean that when more than one substituent exists, the substituents may be the same or different;.
“Alkyl” shall mean straight, cyclic and branched-chain alkyl. Unless otherwise stated, the alkyl group will contain 1-20 carbon atoms. Unless otherwise stated, the alkyl group may be optionally substituted with one or more groups such as halogen, OH, CN, mercapto, nitro, amino, C1-C8-alkyl, C1-C8-alkoxyl, C1-C8-alkylthio, C1-C8-alkyl-amino, di(C1-C8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, C1-C8-alkyl-CO—O—, C1-C8-alkyl-CO—NH—, carboxamide, hydroxamic acid, sulfonamide, sulfonyl, thiol, aryl, aryl(c1-c8)alkyl, heterocyclyl, and heteroaryl.
“Alkoxy” shall mean -0-alkyl and unless otherwise stated, it will have 1-8 carbon atoms.
The term “bioisostere” is defined as “groups or molecules which have chemical and physical properties producing broadly similar biological properties.” (Burger's Medicinal Chemistry and Drug Discovery, M. E. Wolff, ed. Fifth Edition, Vol. 1, 1995, Pg. 785).
“Halogen” shall mean fluorine, chlorine, bromine or iodine; “PH” or “Ph” shall mean phenyl; “Ac” shall mean acyl; “Bn” shall mean benzyl.
The term “acyl” as used herein, whether used alone or as part of a substituent group, means an organic radical having 2 to 6 carbon atoms (branched or straight chain) derived from an organic acid by removal of the hydroxyl group. The term “Ac” as used herein, whether used alone or as part of a substituent group, means acetyl.
“Aryl” or “Ar,” whether used alone or as part of a substituent group, is a carbocyclic aromatic radical including, but not limited to, phenyl, 1- or 2-naphthyl and the like. The carbocyclic aromatic radical may be substituted by independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, C1-C8-alkyl, C1-C8-alkoxyl, C1-C8-alkylthio, C1-C8-alkyl-amino, di(C1-C8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, C1-C8-alkyl-CO—O—, C1-C8-alkyl-CO—NH—, or carboxamide. Illustrative aryl radicals include, for example, phenyl, naphthyl, biphenyl, fluorophenyl, difluorophenyl, benzyl, benzoyloxyphenyl, carboethoxyphenyl, acetylphenyl, ethoxyphenyl, phenoxyphenyl, hydroxyphenyl, carboxyphenyl, trifluoromethylphenyl, methoxyethylphenyl, acetamidophenyl, tolyl, xylyl, dimethylcarbamylphenyl and the like. “Ph” or “PH” denotes phenyl.
Whether used alone or as part of a substituent group, “heteroaryl” refers to a cyclic, fully unsaturated radical having from five to ten ring atoms of which one ring atom is selected from S, O, and N; 0-2 ring atoms are additional heteroatoms independently selected from S, O, and N; and the remaining ring atoms are carbon. The radical may be joined to the rest of the molecule via any of the ring atoms. Exemplary heteroaryl groups include, for example, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrroyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isoxazolyl, thiadiazolyl, triazolyl, triazinyl, oxadiazolyl, thienyl, furanyl, quinolinyl, isoquinolinyl, indolyl, isothiazolyl, 2-oxazepinyl, azepinyl, N-oxo-pyridyl, 1-dioxothienyl, benzothiazolyl, benzoxazolyl, benzothienyl, quinolinyl-N-oxide, benzimidazolyl, benzopyranyl, benzisothiazolyl, benzisoxazolyl, benzodiazinyl, benzofurazanyl, benzothiopyranyl, indazolyl, indolizinyl, benzofuryl, chromonyl, coumarinyl, cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridinyl, furopyridinyl (such as furo[2,3-c]pyridinyl, furo[3,2-b]pyridinyl, or furo[2,3-b]pyridinyl), imidazopyridinyl (such as imidazo[4,5-b]pyridinyl or imidazo[4,5-c]pyridinyl), naphthyridinyl, phthalazinyl, purinyl, pyridopyridyl, quinazolinyl, thienofuryl, thienopyridyl, thienothienyl, and furyl. The heteroaryl group may be substituted by independent replacement of 1 to 5 of the hydrogen atoms thereon with halogen, OH, CN, mercapto, nitro, amino, C1-C8-alkyl, C1-C8-alkoxyl, C1-C8-alkylthio, C1-C8-alkyl-amino, di(C1-C8-alkyl)amino, (mono-, di-, tri-, and per-) halo-alkyl, formyl, carboxy, alkoxycarbonyl, C1-C8-alkyl-CO—O—, C1-C8-alkyl-CO—NH—, or carboxamide. Heteroaryl may be substituted with a mono-oxo to give for example a 4-oxo-1H-quinoline.
The terms “heterocycle,” “heterocyclic,” and “heterocyclo” refer to an optionally substituted, fully or partially saturated cyclic group which is, for example, a 4- to 7-membered monocyclic, 7- to 11-membered bicyclic, or 10- to 15-membered tricyclic ring system, which has at least one heteroatom in at least one carbon atom containing ring. Each ring of the heterocyclic group containing a heteroatom may have 1, 2, or 3 heteroatoms selected from nitrogen atoms, oxygen atoms, and sulfur atoms, where the nitrogen and sulfur heteroatoms may also optionally be oxidized. The nitrogen atoms may optionally be quaternized. The heterocyclic group may be attached at any heteroatom or carbon atom.
Exemplary monocyclic heterocyclic groups include pyrrolidinyl; oxetanyl; pyrazolinyl; imidazolinyl; imidazolidinyl; oxazolyl; oxazolidinyl; isoxazolinyl; thiazolidinyl; isothiazolidinyl; tetrahydrofuryl; piperidinyl; piperazinyl; 2-oxopiperazinyl; 2-oxopiperidinyl; 2-oxopyrrolidinyl; 4-piperidonyl; tetrahydropyranyl; tetrahydrothiopyranyl; tetrahydrothiopyranyl sulfone; morpholinyl; thiomorpholinyl; thiomorpholinyl sulfoxide; thiomorpholinyl sulfone; 1,3-dioxolane; dioxanyl; thietanyl; thiiranyl; and the like. Exemplary bicyclic heterocyclic groups include quinuclidinyl; tetrahydroisoquinolinyl; dihydroisoindolyl; dihydroquinazolinyl (such as 3,4-dihydro-4-oxo-quinazolinyl); dihydrobenzofuryl; dihydrobenzothienyl; dihydrobenzothiopyranyl; dihydrobenzothiopyranyl sulfone; dihydrobenzopyranyl; indolinyl; isochromanyl; isoindolinyl; piperonyl; tetrahydroquinolinyl; and the like.
Substituted aryl, substituted heteroaryl, and substituted heterocycle may also be substituted with a second substituted-aryl, a second substituted-heteroaryl, or a second substituted-heterocycle to give, for example, a 4-pyrazol-1-yl-phenyl or 4-pyridin-2-yl-phenyl.
Designated numbers of carbon atoms (e.g., C1-8) shall refer independently to the number of carbon atoms in an alkyl or cycloalkyl moiety or to the alkyl portion of a larger substituent in which alkyl appears as its prefix root.
Unless specified otherwise, it is intended that the definition of any substituent or variable at a particular location in a molecule be independent of its definitions elsewhere in that molecule. It is understood that substituents and substitution patterns on the compounds of this invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as those methods set forth herein.
Where the compounds according to this invention have at least one stereogenic center, they may accordingly exist as enantiomers. Where the compounds possess two or more stereogenic centers, they may additionally exist as diastereomers. Furthermore, some of the crystalline forms for the compounds may exist as polymorphs and as such are intended to be included in the present invention. In addition, some of the compounds may form solvates with water (i.e., hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention.
Some of the compounds of the present invention may have trans and cis isomers. In addition, where the processes for the preparation of the compounds according to the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as preparative chromatography. The compounds may be prepared as a single stereoisomer or in racemic form as a mixture of some possible stereoisomers. The non-racemic forms may be obtained by either synthesis or resolution. The compounds may, for example, be resolved into their components enantiomers by standard techniques, such as the formation of diastereomeric pairs by salt formation. The compounds may also be resolved by covalent linkage to a chiral auxiliary, followed by chromatographic separation and/or crystallographic separation, and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using chiral chromatography.
This invention will be better understood by reference to the Experimental Details which follow, but those skilled in the art will readily appreciate that these are only illustrative of the invention as described more fully in the claims which follow thereafter. Additionally, throughout this application, various publications are cited. The disclosure of these publications is hereby incorporated by reference into this application to describe more fully the state of the art to which this invention pertains.
Experimental Details
I. General Synthetic Schemes
Representative compounds of the present invention can be synthesized in accordance with the general synthetic methods described below and illustrated in the following general schemes. The products of some schemes can be used as intermediates to produce more than one of the instant compounds. The choice of intermediates to be used to produce subsequent compounds of the present invention is a matter of discretion that is well within the capabilities of those skilled in the art.
Procedures described in Schemes 1 to 7, wherein R3a, R3b, R3c, and R3d are independently any R3 group, and R1, R2, R3, and R4 are as described above, can be used to prepare compounds of the invention.
The substituted pyrimidines 1 can be prepared as shown in Scheme 1. The indanone or indandione 2 or the indene ester 3 can be condensed with an aldehyde to yield the substituted benzylidenes 4 (Bullington, J. L; Cameron, J. C.; Davis, J. E.; Dodd, J. H.; Harris, C. A.; Henry, J. R.; Pellegrino-Gensey, J. L.; Rupert, K. C.; Siekierka, J. J. Bioorg. Med. Chem. Lett. 1998, 8, 2489; Petrow, V.; Saper, J.; Sturgeon, B. J. Chem. Soc. 1949, 2134). This is then condensed with guanidine carbonate to form the indenopyrimidine 1.




Alternatively, the pyrimidine compounds can be prepared as shown in Scheme 2. Sulfone 6 can be prepared by oxidation of the thiol ether 5 and the desired amines 7 can be obtained by treatment of the sulfone with aromatic amines.




Pyrimidines with substituents on the fused aromatic ring could also be synthesized by the following procedure (Scheme 3). The synthesis starts with alkylation of furan with allyl bromide to provide 2-allylfuran. Diels-Alder reaction of 2-allylfuran with dimethylacetylene dicarboxylate followed by deoxygenation (Xing, Y. D.; Huang, N. Z. J. Org. Chem. 1982, 47, 140) provided the phthalate ester 8. The phthalate ester 8 then undergoes a Claisen condensation with ethyl acetate to give the styryl indanedione 9 after acidic workup (Buckle, D. R.; Morgan, N. J.; Ross, J. W.; Smith, H.; Spicer, B. A. J. Med. Chem. 1973, 16, 1334). The indanedione 9 is then converted to the dimethylketene dithioacetal 10 using carbon disulfide in the presence of KF. Addition of Grignard reagents to the dithioacetal 10 and subsequent reaction with guanidine provides the pyrimidines 11 as a mixture of isomers.




Dihydroxylation and oxidation give the aromatic aldehydes 13 that can be reductively aminated to provide amines 14. The other isomer can be treated in a similar manner.




3-Dicyanovinylindan-1-one (15) (Scheme 5) was obtained using the published procedure (Bello, K. A.; Cheng, L.; Griffiths, J. J. Chem. Soc., Perkin Trans. II 1987, 815). Reaction of 3-dicyanovinylindan-1-one with an aldehyde in the presence of ammonium hydroxide produced dihydropyridines 16 (El-Taweel, F. M. A.; Sofan, M. A.; E.-Maati, T. M. A.; Elagamey, A. A. Boll. Chim. Farmac. 2001, 140, 306). These compounds were then oxidized to the corresponding pyridines 17 using chromium trioxide in refluxing acetic acid.




The ketone of pyridines 17 can be reduced to provide the benzylic alcohols 18. Alternatively, the nitriles can be hydrolyzed with sodium hydroxide to give the carboxylic acids 19 (Scheme 6).




The acids can then be converted to carboxylic esters 20 or amides 21 using a variety of methods. In general, the esters 20 are obtained by treatment with silver carbonate followed by an alkyl chloride or by coupling with diethylphosphoryl cyanide (DEPC) and the appropriate alcohol (Okawa, T.; Toda, M.; Eguchi, S.; Kakehi, A. Synthesis 1998, 1467). The amides 21 are obtained by coupling the carboxylic acid with the appropriate amine in the presence of DEPC or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCl). Esters 20 can also be obtained by first reacting the carboxylic acids 19 with a dibromoalkane followed by displacement of the terminal bromide with an amine (Scheme 7).




II. Specific Compound Syntheses

Specific compounds which are representative of this invention can be prepared as per the following examples. No attempt has been made to optimize the yields obtained in these reactions. Based on the following, however, one skilled in the art would know how to increase yields through routine variations in reaction times, temperatures, solvents and/or reagents.
The products of certain syntheses can be used as intermediates to produce more than one of the instant compounds. In those cases, the choice of intermediates to be used to produce compounds of the present invention is a matter of discretion that is well within the capabilities of those skilled in the art.
EXAMPLE 1
Synthesis of Benzylidene 4
(R2=2-furyl, R3a═F, R3b, R3c, R3d═H)
A mixture of 3 (3.0 g, 11.69 mmol) and 2-furaldehyde (1.17 g, 12.17 mmol) in 75 mL of ethanol and 3 mL of concentrated hydrogen chloride was allowed to stir at reflux for 16 hours. The reaction was then cooled to room temperature, and the resulting precipitate was filtered off, washed with ethanol, diethyl ether, and air dried to afford 1.27 g (45%) of product.
EXAMPLE 2
Synthesis of Indenopyrimidine 1
(R2=2-furyl, R3a═F, R3b, R3c, R3d═H)
A mixture of 4 (0.5 g, 2.06 mmol), guanidine carbonate (0.93 g, 5.16 mmol), and 20.6 mL of 0.5 M sodium methoxide in methanol was stirred at reflux for 16 hours. The reaction mixture was cooled to room temperature, and diluted with water. The resulting precipitate was collected, washed with water, ethanol, diethyl ether, and then dried. Crude material was then purified over silica gel to afford 0.024 g (4%) of product. MS m/z 282.0 (M+H).
EXAMPLE 3
Synthesis of 2-Amino-4-methanesulfonyl-indeno[1,2-d]pyrimidin-5-one
To a suspension of 5 (Augustin, M.; Groth, C.; Kristen, H.; Peseke, K.; Wiechmann, C. J. Prakt. Chem. 1979, 321, 205) (1.97 g, 8.10 mmol) in MeOH (150 mL) was added a solution of oxone (14.94 g, 24.3 mmol) in H2O (100 mL). The mixture was stirred at room temperature overnight then diluted with cold H2O (500 mL), made basic with K2CO3 and filtered. The product was washed with water and ether to give 0.88 g (40%) of sulfone 6. MS m/z 297.9 (M+Na).
EXAMPLE 4
Synthesis of Aminopyrimidine 7
(R2═NHPh, R3═H)
A mixture of sulfone 6 (0.20 g, 0.73 mmol) and aniline (0.20 g, 2.19 mmol) in N-methylpyrrolidinone (3.5 mL) was heated to 100° C. for 90 minutes. After cooling to room temperature, the mixture was diluted with EtOAc (100 mL), washed with brine (2×75 mL) and water (2×75 mL), and dried over Na2SO4. After filtration and concentration in vacuo, the residue was purified by column chromatography eluting with 0-50% EtOAc in hexane to yield 0.0883 g (42%) of product 7. MS m/z 289.0 (M+H).
EXAMPLE 5
Synthesis of Phthalate Ester 8
A 1.37 M hexanes solution of n-BuLi (53.6 mL, 73.4 mmol) was added to a cold, −78° C., THF solution (100 mL) of furan (5.3 mL, 73.4 mmol) and the reaction was then warmed to 0° C. After 1.25 h at 0° C. neat allyl bromide (7.9 mL, 91.8 mmol) was added in one portion. After 1 h at 0° C., saturated aqueous NH4Cl was added and the layers were separated. The aqueous phase was extracted with EtOAc and the combined organics were washed with water and brine, dried over Na2SO4, and concentrated to give 4.6 g (58%) of 2-allylfuran which was used without further purification.
The crude allyl furan (4.6 g, 42.6 mmol) and dimethylacetylene dicarboxylate (5.2 mL, 42.6 mmol) were heated to 90° C. in a sealed tube without solvent. After 6 h at 90° C. the material was cooled and purified by column chromatography eluting with 25% EtOAc in hexanes to give 5.8 g (54%) of the oxabicycle as a yellow oil. MS m/z 251 (M+H).
Tetrahydrofuran (60 mL) was added dropwise to neat TiCl4 (16.5 mL, 150.8 mmol) at 0° C. A 1.0 M THF solution of LiAlH4 (60.3 mL, 60.3 mmol) was added dropwise, changing the color of the suspension from yellow to a dark green or black suspension. Triethylamine (2.9 mL, 20.9 mmol) was added and the mixture was refluxed at 75-80° C. After 45 min, the solution was cooled to rt and a THF solution (23 mL) of the oxabicycle (5.8 g, 23.2 mmol) was added to the dark solution. After 2.5 h at rt, the solution was poured into a 20% aq. K2CO3 solution (200 mL) and the resulting suspension was filtered. The precipitate was washed several times with CH2Cl2 and the filtrate layers were separated. The aqueous phase was extracted with CH2Cl2 and the combined organics were washed with water and brine, dried over Na2SO4, concentrated, and purified by column chromatography eluting with 25% EtOAc in hexanes to give 3.5 g (64%) of the phthalate ester 8 as a yellow oil. MS m/z 235 (M+H).
EXAMPLE 6
Synthesis of Indanedione 9
A 60% dispersion of sodium hydride in mineral oil (641 mg, 16.0 mmol) was added to an EtOAc solution (3.5 mL) of the phthalate ester 8 (2.5 g, 10.7 mmol), and the resulting slurry was refluxed. After 1 h the solution became viscous so an additional 7.5 mL of EtOAc was added. After 4 h at reflux the suspension was cooled to rt and filtered to give a yellow solid. This solid was added portionwise to a solution of HCl (25 mL water and 5 mL conc. HCl) at 80° C. The suspension was heated for an additional 30 min at 80° C., cooled to rt, and filtered to give 1.2 g (60%) of the indanedione 9 as a yellow solid. MS m/z 187 (M+H).
EXAMPLE 7
Synthesis of Dimethylketene Dithioacetal 10
Solid potassium fluoride (7.5 g, 129.1 mmol) was added to a 0° C. solution of indanedione 9 (1.2 g, 6.5 mmol) and CS2 (0.47 mL, 7.8 mmol) in DMF (10 mL). The cold bath was removed and after 30 min neat iodomethane (1.00 mL, 16.3 mmol) was added. After 5 h at rt, the suspension was diluted with EtOAc and then washed with water and brine. The organic layer was dried over Na2SO4, concentrated, and purified by column chromatography eluting with 20% EtOAc in hexanes to give 1.4 g (75%) of the dimethylketene dithioacetal 10 as a yellow solid. MS m/z 291 (M+H).
EXAMPLE 8
Synthesis of Pyrimidine 11
(R2═Ph, R3a═CHCHCH3, R3d═H)
A 2.0 M solution of PhMgCl in THF (13 mL, 25.7 mmol) was added to a −78° C. solution of dimethylketene dithioacetal 10 (5.7 g, 19.8 mmol) in 200 mL of THF. After 3 h at −78° C., saturated aqueous NH4Cl was added and the layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts were washed with water and brine, dried over Na2SO4, concentrated, and purified by column chromatography eluting with 20% EtOAc in hexanes to give 4.9 g (77%) of the thioenol ether as a yellow solid. MS m/z 321 (M+H).
Solid guanidine hydrochloride (1.5 g, 15.3 mmol) was added to a solution of the thioenol ether (4.9 g, 15.3 mmol) and K2CO3 (2.6 g, 19.1 mmol) in 30 mL of DMF and the solution was heated to 80° C. After 6 h at 80° C., the solution was diluted with EtOAc and washed with water and brine. The organic layer was dried over Na2SO4, concentrated, and purified by column chromatography eluting with 40% EtOAc in hexanes to give 4.6 g (96%) of the pyrimidine regioisomers 11 as yellow solids. MS m/z 314 (M+H).
EXAMPLE 9
Synthesis of Aldehyde 13
(R2═Ph)
Solid MeSO2NH2 (277 mg, 2.9 mmol) was added to a t-BuOH:H2O (1:1) solution (30 mL) of AD-mix-α (4.0 g). The resulting yellow solution was added to an EtOAc solution (15 mL) of the pyrimidine (910 mg, 2.9 mmol). After 3 days, solid sodium sulfite (4.4 g, 34.9 mmol) was added. After stirring for 1.5 h, the heterogeneous solution was diluted with EtOAc and the layers were separated. The aqueous phase was extracted with EtOAc and the combined extracts were washed with water and brine, dried over Na2SO4, concentrated, and purified by column chromatography eluting with 100% EtOAc to give 710 mg (70%) of the intermediate diol 12. MS m/z 348 (M+H).
Solid HIO4—2H2O (933 mg, 4.1 mmol) was added to a 0° C. solution of diol 12 (710 mg, 2.1 mmol) in THF. After 1.5 h at 0° C., the solution was diluted with EtOAc and the organic phase was washed with saturated aqueous NaHCO3, water, and brine. The organic layer was dried over Na2SO4 and concentrated to give 603 mg (98%) of aldehyde 13 as a yellow solid that was used without further purification. MS m/z 302 (M+H).
EXAMPLE 10
Synthesis of Amine 14 via Reductive Amination
(R3a═N(—CH2CH2OCH2CH2—)
Solid NaBH(OAc)3 (53 mg, 0.25 mmol) was added to a solution of aldehyde 13 (50 mg, 0.17 mmol), morpholine (0.034 mL, 0.34 mmol), and ACOH (0.014 mL, 0.25 mmol) in 1 mL of THF. After 3 d the solution was filtered and concentrated. The resulting material was dissolved in CH2Cl2 and washed with saturated aqueous NaHCO3 and brine, dried over Na2SO4, concentrated, and purified by column chromatography eluting with 0-10% MeOH in CH2Cl2 to give 38 mg (60%) of the amine 14 as a yellow solid. MS m/z 373 (M+H). The product was dissolved in a minimum amount of CH2Cl2 and treated with 1.0 M HCl in ether to obtain the hydrochloride salt.
EXAMPLE 11
Cyclization to Form Dihydropyridine 16
(R2=2-furyl, R3═H)
To a solution of 3-dicyanovinylindan-1-one (4.06 g, 20.9 mmol) in 200 mL of ethanol was added 2-furaldehyde (3.01 g, 31.4 mmol) and 25 mL of conc. NH4OH. The solution was heated to reflux for 2 h and allowed to cool to rt overnight. The mixture was concentrated in vacuo to remove ethanol. The residue was filtered and washed with water. The purple solid obtained was dried to yield 5.92 g (89%). MS m/z 290 (M++1).
EXAMPLE 12
Oxidation of Dihydropyridine 16 to Pyridine 17
(R2=2-furyl, R3═H, R4═NH2, R5═CN, X═O)
To a refluxing solution of dihydropyridine 16 (5.92 g, 20.4 mmol) in acetic acid (100 mL) was added a solution of chromium (VI) oxide (2.05 g, 20.4 mmol) in 12 mL of water. After 10 minutes at reflux, the reaction was diluted with water until a precipitate started to form. The mixture was cooled to room temperature and filtered. The residue was washed with water to give 4.64 g (79%) of a brown solid. MS m/z 288 (M++1).
EXAMPLE 13
Reduction of Ketone 17 to Alcohol 18
(R2═2-furyl, R3═H, R4═NH2, R5═CN, X═H, OH)
To a 0° C. solution of ketone 17 (0.115 g, 0.40 mmol) in 12 mL of THF was added a 1.0 M LiAlH4 solution in THF (0.40 mL, 0.40 mmol). The reaction was stirred at 0° C. for 1 h. The reaction was quenched by the addition of ethyl acetate (1.5 mL), water (1.5 mL), 10% aq. NaOH (1.5 mL), and saturated aq. NH4Cl (3.0 mL). The mixture was extracted with ethyl acetate (3×35 mL), washed with brine, and dried over sodium sulfate. The remaining solution was concentrated to yield 0.083 g (72%) of a yellow solid. MS m/z 290 (M++1).
EXAMPLE 14
Hydrolysis of Nitrile 17 to Carboxylic Acid 19
(R2=2-furyl, R3═H, R4═NH2, R5═COOH, X═O)
To a mixture of nitrile 17 (0.695 g, 2.42 mmol) and ethanol (30 mL) was added 5 mL of 35% aqueous sodium hydroxide. The resulting mixture was heated to reflux overnight. After cooling to rt, the solution was poured into water and acidified with 1 N HCl. The resulting precipitate was isolated by filtration and washed with water to yield 0.623 g (84%) of a brown solid. MS m/z 329 (M++23).
EXAMPLE 15
Synthesis of Carboxylic Ester 20 with Silver Carbonate
(R2=2-furyl, R3═H, R4═NH2, R5═CO2CH2CH2NMe2, X═O)
A suspension of carboxylic acid 19 (5.0 g, 16.3 mmol), silver carbonate (5.8 g, 21.2 mmol), and tetrabutylammonium iodide (1.5 g, 4.1 mmol) in 80 mL of DMF was heated to 90° C. After 1 h, the mixture was cooled to rt and 2-(dimethylamino)ethylchloride hydrochloride (2.4 g, 16.3 mmol) was added and the mixture was heated to 100° C. After 7 h, the reaction was filtered while hot, concentrated and purified by column chromatography eluting with 0-10% MeOH/CH2Cl2 to yield 0.160 g (3%) of a yellow solid. MS m/z 378 (M++1). The product was dissolved in a minimum of dichloromethane and treated with 1.0 M HCl in ether to obtain the hydrochloride salt.
EXAMPLE 16
Synthesis of Carboxylic Ester 20 with DEPC
(R2═2-furyl, R3═H, R4═NH2, R5═CO2CH2CH(—CH2CH2CH2(Me)N—),
X═O)
To a mixture of carboxylic acid 19 (0.40 g, 1.3 mmol) and (S)-1-methyl-2-pyrrolidinemethanol (0.50 mL, 3.9 mmol) in DMF (30 mL) was added 0.20 mL (1.3 mmol) of diethylphosphoryl cyanide and triethylamine (0.20 mL, 1.3 mmol). The reaction was stirred at 0° C. for one hour and then heated up to approximately 70° C. overnight. The reaction was then cooled to rt and diluted with ethyl acetate. The organic mixture was washed with saturated aqueous NaHCO3, water, and brine. After being dried with sodium sulfate, the solution was concentrated. The residue was purified by column chromatography eluting with 10-100% ethyl acetate in hexane and then preparative TLC eluting with 2% MeOH in dichloromethane to yield 1.9 mg (0.4%) of.a yellow solid. MS m/z 404 (M++1).
EXAMPLE 17
Synthesis of Carboxylic Amide 21 with DEPC
(R2=2-furyl, R3═H, R4═NH2, R5═CO2CH2CH(—CH2CH2CH2(Me)N—), X═O)
To a mixture of carboxylic acid 19 (0.25 g, 0.82 mmol) and N,N,N′-trimethylethylenediamine (0.14 mL, 1.08 mmol) in DMF (20 mL) was added 0.12 mL (0.82 mmol) of diethylphosphoryl cyanide and triethylamine (0.11 mL, 0.82 mmol). The reaction was stirred at 0° C. for one hour and then heated up to approximately 60° C. overnight. The reaction was then cooled to rt and diluted with ethyl acetate. The organic mixture was washed with saturated aqueous NaHCO3, water, and brine. After being dried with magnesium sulfate, the solution was concentrated. The residue was purified by column chromatography eluting with 0-10% methanol in dichloromethane and then preparative TLC eluting with 1% MeOH in dichloromethane to yield 3.3 mg (10%) of a yellow solid. MS m/z 391 (M++1). The product was dissolved in a minimum of diethyl ether and treated with 1.0 M HCl in ether to obtain the hydrochloride salt.
EXAMPLE 18
Synthesis of Carboxylic Amide 21 with EDCl
(R2=2-furyl, R3═H, R4═NH2, R5═CON(—CH2CH2NMeCH2CH2—). X═O)
A mixture of carboxylic acid 19 (0.300 g, 0.979 mmol), N-methylpiperazine (0.295 g, 2.94 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.563 g, 2.94 mmol) 1-hydroxybenzotriazole hydrate (0.397 g, 2.94 mmol), triethylamine (0.298 g, 2.94 mmol) in DMF (8 mL) was stirred at rt overnight. The mixture was then diluted with water and extracted several times with ethyl acetate. The combined organics were washed twice with brine and then dried over sodium sulfate. The solution was concentrated and then purified by column chromatography to afford 0.092 g (2%) of solid. MS m/z 389 (M++1). The product was treated with 1.0 M HCl in ether to obtain the hydrochloride salt.
EXAMPLE 19
Synthesis of Carboxylic Ester 20 via a Dibromoalkane
(R2═Ph, R3═H, R4═NH2 R5═CO2CH2CH2CH2NMe2, X═O)
To a solution of carboxylic acid 19 (0.100 g, 0.32 mmol) in DMF (1.5 mL) was added 60% NaH dispersion in mineral oil (0.013 g, 0.32 mmol). After 10 min at rt, 1,3-dibromopropane (0.035 mL, 0.35 mmol) was added and the solution was stirred at rt for 17 h. After concentration, the residue was purified via column chromatography eluting with 40% ethyl acetate in hexanes to yield 0.014 g (9%) of a yellow solid. MS m/z 437 (M++1).
To a solution of the yellow solid (0.014 mg, 0.03 mmol) in a sealed tube was added a 40% aqueous solution of dimethylamine (0.5 mL, 3.0 mmol). The tube was heated to 75° C. for 2 h before concentrating. The residue was purified by column chromatography eluting with 0-10% methanol in dichloromethane to yield 0.009 g (70%) of a yellow solid. MS m/z 402 (M++1). The product was dissolved in a minimal amount of CH2Cl2 and treated with 1 N HCl in ether to obtain the hydrochloride salt.
Following the general synthetic procedures outlined above and in Examples 1-19, the compounds of Table 1 below were prepared.







TABLE 1













 















No.
R2
R3a
R3b
R3c
R3d
R4
X
MS (M + 1)


















1
4-MeOPh
H
H
H
H
NH2
CH2
290


2
4-MeOPh
H
H
H
H
NH2
CO
304


3
2-furyl
H
H
H
H
NH2
CO
264


4
2-furyl
H
H
H
H
NH2
CH2
250


5
3-pyridyl
H
H
H
H
NH2
CO
297 (+Na)


6
4-pyridyl
H
H
H
H
NH2
CO
275


 


7




H
H
H
H
NH2
CO
281


 


8
4-ClC6H4
H
H
H
H
NH2
CO
308


9
3-NO2C6H4
H
H
H
H
NH2
CO
319


10
Ph
H
H
H
H
NH2
CO
274


11
3-MeOC6H4
H
H
H
H
NH2
CO
304


12
2-MeOC6H4
H
H
H
H
NH2
CO
304


13
3-HOC6H4
H
H
H
H
NH2
CO
290


14
2-thiophenyl
H
H
H
H
NH2
CO
302


15
3-thiophenyl
H
H
H
H
NH2
CO
302


16
2-furyl
H
Br
H
H
NH2
CO
342


17
2-furyl
OH
H
H
H
NH2
CO
280


18
SCH3
NH2
H
H
H
NH2
CO
259


19
3-FC6H4
H
H
H
H
NCHNMe2
CO
347


20
2-furyl
NH2
H
H
H
NH2
CO
279


21
2-furyl
H
H
H
NH2
NH2
CO
279


22
2-furyl
H
CF3
H
H
NH2
CO
332


23
2-furyl
H
H
CF3
H
NH2
CO
332


24
Ph
H
H
H
H
NHMe
CO
288


25
2-furyl
H
Cl
Cl
H
NH2
CO
332


26
2-furyl
Cl
H
H
Cl
NH2
CO
332


27
Ph
H
H
H
H
N(CH2)2NEt2
CO
373


28
3,4-F2C6H3
H
H
H
H
NH2
CO
310


29
3,5-F2C6H3
H
H
H
H
NH2
CO
310


 


30




H
H
H
H
NH2
CO
305


 


31
3,4,5-F3C2H2
H
H
H
H
NH2
CO
340 (M + Na)


 


32
Ph




H
H
H
NH2
CO
348


 


33
Ph
H
H
H




NH2
CO
348


 


34




H
H
H
H
NH2
CO
333


 


35
2-furyl
H
H
Br
H
NH2
CO
342/344


36
2-furyl
H
H
H
F
NH2
CO
282


37
2-furyl
MeO
H
H
H
NH2
CO
294


38
4-FC6H4
H
H
H
H
NH2
CO
292


39
3-FC6H4
H
H
H
H
NH2
CO
292


40
SO2Me
H
H
H
H
NH2
CO
298


41
Sme
H
H
H
H
NH2
CO
266


42
Ome
H
H
H
H
NH2
CO
477










(2M + Na)


43
NHPh
H
H
H
H
NH2
CO
289


44
3-furyl
H
H
H
H
NH2
CO
264


45
5-methyl-2-furyl
H
H
H
H
NH2
CO
278


46
2-furyl
OCH2CH2NHCO2tBu
H
H
H
NH2
CO
437


47
Ph
H
H
H
H
Me
CO
297


48
Ph
H
H
H
H
OMe
CO
291


49
Ph
CH2NMeCH2CH2NMe2
H
H
H
NH2
CO
388


 


50
Ph




H
H
H
NH2
CO
386


 


51
Ph




H
H
H
NH2
CO
373


 


52
Ph
CH2NEt2
H
H
H
NH2
CO
359


 


53
Ph




H
H
H
NH2
CO
371


 


54
Ph




H
H
H
NH2
CO
429


 


55
Ph




H
H
H
NH2
CO
443


 


56
Ph
CH2NMeCH2CO2Me
H
H
H
NH2
CO
389


 


57
Ph




H
H
H
NH2
CO
401


 


58
Ph




H
H
H
NH2
CO
416


 


59
Ph




H
H
H
NH2
CO
414


 


60
Ph




H
H
H
NH2
CO
486


 


61
Ph




H
H
H
NH2
CO
422


 


62
Ph




H
H
H
NH2
CO
397
















TABLE 2













 























MS


No.
X
R2
R3a
R3b
R3c
R3d
R1
(M + 1)


















63
CO
2-furyl
H
H
H
H
CN
288


64
CO
Ph
H
H
H
H
CN
298


65
CO
Ph
H
H
H
H
COOH
315










(M − 1)


66
CO
3-furyl
H
H
H
H
CN
288


67
CO
3-FC6H4
H
H
H
H
CN
316


68
CO
3-pyridyl
H
H
H
H
CN
299


69
CO
2-furyl
H
H
H
H
COOH
305










(M − 1)


70
CO
2-furyl
H
H
H
H
CO2CH2CH2NMe2
378


71
CO
4-FC6H4
H
H
H
H
CN
316


72
CO
2-thiophenyl
H
H
H
H
CN
304


73
CO
3-thiophenyl
H
H
H
H
CN
304


74
CO
3-MeOC6H4
H
H
H
H
CN
328


75
CO
2-imidazolyl
H
H
H
H
CN
288


76
CO
2-furyl
H
H
H
H
CONHCH2CH2NMe2
377


77
CO
2-furyl
H
H
H
H
CONMeCH2CH2NMe2
391


78
CO
2-furyl
H
H
H
H
CONHCHMeCH2NMe2
391


79
CO
2-furyl
F
F
F
F
CN
358










(M − 1)


 


80
CO
2-furyl
H
H
H
H




389


 


81
CO
Ph
H
H
H
H
CO2CH2CH2NMe2
388


 


82
CO
2-furyl
H
H
H
H




404


 


83
CO
Ph
H
H
H
H




457


 


84
CO
Ph
H
H
H
H




444


 


85
CO
Et
H
H
H
H
CN
250


86
CO
i-Bu
H
H
H
H
CN
278


87
CO
Ph
H
H
H
H
CO2CH2CH2CH2NMe2
402


 


88
CO
Ph
H
H
H
H




414


 


89
CHOH
2-furyl
H
H
H
H
CN
290


 


90
CO
Ph
H
H
H
H




414


 


91
CO
Ph
H
H
H
H




430


 


92
CO
Ph
H
H
H
H
CO2CH2CHMeCH2NMe2
416


93
CO
3-thiophenyl
H
H
H
H
CO2CH2CH2NMe2
394


94
CO
CH2CH2CHCH2
H
H
H
H
CN
276


95
CO
c-Hex
H
H
H
H
CN
302










(M − 1)


96
CO
2-furyl
H
H
H
H
(S)—CO2CHMeCH2NMe2
392









III. Biological Assays and Activity

Ligand Binding Assay for Adenosine A2a Receptor

Ligand binding assay of adenosine A2a receptor was performed using plasma membrane of HEK293 cells containing human A2a adenosine receptor (PerkinElmer, RB-HA2a) and radioligand [3H]CGS21680 (PerkinElmer, NET1021). Assay was set up in 96-well polypropylene plate in total volume of 200 μL by sequentially adding 20 μL 1:20 diluted membrane, 130 μL assay buffer (50 mM Tris.HCl, pH7.4 10 mM MgCl2, 1 mM EDTA) containing [3H] CGS21680, 50 μL diluted compound (4×) or vehicle control in assay buffer. Nonspecific binding was determined by 80 mM NECA. Reaction was carried out at room temperature for 2 hours before filtering through 96-well GF/C filter plate pre-soaked in 50 mM Tris.HCl, pH7.4 containing 0.3% polyethylenimine. Plates were then washed 5 times with cold 50 mM Tris.HCl, pH7.4, dried and sealed at the bottom. Microscintillation fluid 30 μl was added to each well and the top sealed. Plates were counted on Packard Topcount for [3H]. Data was analyzed in Microsoft Excel and GraphPad Prism programs. (Varani, K.; Gessi, S.; Dalpiaz, A.; Borea, P. A. British Journal of Pharmacology, 1996, 117, 1693)
Adenosine A2a Receptor Functional Assay
CHO-K1 cells overexpressing human adenosine A2a receptors and containing cAMP-inducible beta-galactosidase reporter gene were seeded at 40-50K/well into 96-well tissue culture plates and cultured for two days. On assay day, cells were washed once with 200 μL assay medium (F-12 nutrient mixture/0.1% BSA). For agonist assay, adenosine A2a receptor agonist NECA was subsequently added and cell incubated at 37° C., 5% CO2 for 5 hrs before stopping reaction. In the case of antagonist assay, cells were incubated with antagonists for 5 minutes at R.T. followed by addition of 50 nM NECA. Cells were then incubated at 37° C., 5% CO2 for 5 hrs before stopping experiments by washing cells with PBS twice. 50 μL 1× lysis buffer (Promega, 5× stock solution, needs to be diluted to 1× before use) was added to each well and plates frozen at −20° C. For β-galactosidase enzyme calorimetric assay, plates were thawed out at room temperature and 50 μL 2× assay buffer (Promega) added to each well. Color was allowed to develop at 37° C. for 1 h or until reasonable signal appeared. Reaction was then stopped with 150 μL 1M sodium carbonate. Plates were counted at 405 nm on Vmax Machine (Molecular Devices). Data was analyzed in Microsoft Excel and GraphPad Prism programs. (Chen, W. B.; Shields, T. S.; Cone, R. D. Analytical Biochemistry, 1995, 226, 349; Stiles, G. Journal of Biological Chemistry, 1992, 267, 6451)
Haloperidol-Induced Catalepsy Study in C57bl/6 Mice
Mature male C57bl/6 mice (9-12 week old from ACE) were housed two per cage in a rodent room. Room temperature was maintained at 64-79 degrees and humidity at 30-70% and room lighting at 12 hrs light/12 hrs dark cycle. On the study day, mice were transferred to the study room. The mice were injected subcutaneously with haloperidol (Sigma H1512, 1.0 mg/ml made in 0.3% tartaric acid, then diluted to 0.2 mg/ml with saline) or vehicle at 1.5 mg/kg, 7.5ml/kg. The mice were then placed in their home cages with access to water and food. 30 minutes later, the mice were orally dosed with vehicle (0.3% Tween 80 in saline) or compounds at 10 mg/kg, 10 ml/kg (compounds, 1 mg/ml, made in 0.3% Tween 80 in saline, sonicated to obtain a uniform suspension). The mice were then placed in their home cages with access to water and food. 1 hour after oral dose, the catalepsy test was performed. A vertical metal-wire grid (1.0 cm squares) was used for the test. The mice were placed on the grid and given a few seconds to settle down and their immobility time was recorded until the mice moved their back paw(s). The mice were removed gently from the grid and put back on the grid and their immobility time was counted again. The measurement was repeated three times. The average of three measurements was used for data analysis.
Compound 70 showed 87% inhibition and compound 3 showed 90% inhibition of haloperidol-induced catalepsy when orally dosed at 10 mg/kg.









TABLE 5









Ki (nM)














A2a antagonist
A1 antagonist



No.
A2a binding
function
function
















1
44.64
233.7
52.98



2
2.032
6.868
5.32



3
0.26
0.0066
0.288



4
0.885
2.63
15.57



5
5.355
9.64
27.1



6
3.9
4.56
16.44



7
0.26
0.49
6.89



8
58.41
5.5
11.59



9
20.82
4.85
7.69



10
6.1
0.109
1.2



11
8.85
1.63
2.47



12
33.49
32.52
172.3



13
5.16
35.59
10.35



14
2.19
0.59
3.19



15
3.23
0.258
3.46



16
1.75
0.169
5.22



17
6.3
67.14
111.29



18
317.95
>3000
188.99



19
110.73
20.88
21.64



20
0.05
0.126
0.91



21
0.376
0.053
3.51



22
14.16
0.055
2.75



23
13.58
0.55
1.47



24
30.32
>3000
5.99



25
172.85
5.69
17.44



26
34.57
0.88
3.13



27
146.84
68.28
>1000



28
48.9
3.53
5.86



29
20.95
1.42
4.27



30
31.55
10.15
4.05



31
140.68
15.22
17.5



32
3.55
0.634
9.89



33
0.175
0.34
0.021



34
560.13



35
3.49
0.265
7.09



36
4.37
0.052
2.52



37
2.86
0.143
3.07



38
2.34
0.956
9.44



39
4.92
0.926
2.31



40
2720.46



41
88.01
575.43
>3000



42
118.2
782.18
>10000



43
39.9
3.68
2.34



44
3.93
0.208
7.4



45
4.013
0.005
0.016



46
60.56
490.14
32.54



47
1076.76



48
470.84
>1000
>1000



49
51.12
40.13
119.03



50
80.15
11.31
94.24



51
36.81
3.26
32.92



52
94.41
18.33
107.17



53
64.15
14.25
40.82



54
40.79
3.19
19.56



55
32.82
5.84
19.86



56
25.72
6.81
25.76



57
34.02
15.93
39.29



58
30.65
11.65
60.99



59
40.79
7.94
34.11



60
34.29



61
29.83



62
58.39



63
0.59
0.0002
0.18



64
13.09
0.138
4.61



65
574.71
244.96
163.36



66
4.21
0.069
15.59



67
13.4
0.618
4.37



68
7.59
0.73
34.84



69
2261
90.16
>1000



70
9.89
0.44
20.13



71
17.24
3.39
2.42



72
12.64
2.54
6.24



73
4.925
0.06
9.7



74
14.67
5.7
7.28



75
23.72
1.51
78.33



76
33.03
22.13
>500



77
6.254
0.68
>500



78
17.65
1.58
>500



79
8.03
12.48
>1000



80
69.08
15.86
55.99



81
228.7
29.03
33.63



82
20.24
1.36
29.58



83
200.06
74.87
117.05



84
173.98
24.71
27.42



85
507.72



86
244.07
>1000
39.26



87
98.93
39.45
>300



88
129.6
48.87
>300



89
5.85
1.12
11.16



90
202.17
57.7
>300



91
208.32
22.07
14.67



92
38.82
13.9
32.88



93
64.05
23.57
104.31



94
49.55
>1000
35.99



95
338.13
>1000
110.22



96
48.55
10.08
52.45

## Claims

1. A compound having the structure of Formula II




or a pharmaceutically acceptable salt thereof, wherein
(a) R1 is selected from the group consisting of
(i) —COR5, wherein;
R5 is selected from H, optionally substituted C1-8 straight or branched chain alkyl, optionally substituted aryl and optionally substituted arylalkyl;
wherein;
the substituents on the alkyl, aryl and arylalkyl group are selected from C1-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, or NR7R8 wherein R7 and R8 are independently selected from the group consisting of hydrogen, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, benzyl or aryl;



(ii)COOR5, wherein R5 is as defined above;
(iii) cyano;
(iv) —CONR9R10 wherein
R9 and R10 are independently selected from H, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, trifluoromethyl, hydroxy, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl or aryl;
wherein the alkyl, cycloalkyl, alkoxy, acyl, alkylcarbonyl, carboxyl, arylalkyl and aryl, may be substituted with carboxyl, alkyl, aryl, substituted aryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, amino, alkoxy or arylalkyl;

(v) optionally substituted C1-8 straight or branched chain alkyl;
wherein the substituents on the alkyl, group are selected from C1-8 alkoxy, phenylacetyloxy, hydroxy, halogen, p-tosyloxy, mesyloxy, amino, cyano, carboalkoxy, carboxyl, aryl, sulfonyl, thiol, alkylthio, or NR7R8 wherein R7 and R8 are as defined above;

(b) R2 is selected from the group consisting of optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl and optionally substituted C3-7 cycloalkyl, C1-8 alkoxy, aryloxy, C1-8 alkylsulfonyl, arylsulfonyl, arylthio, C1-8 alkylthio,
with the proviso that the heteroaryl or heterocyclyl of variable R2 is furyl, pyridyl or thiazole only, or —NR24R25 
wherein;
R24 and R25 are independently selected from H, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, carboxyalkyl, aryl, heteroaryl, and heterocyclyl or R24 and R25 taken together with the nitrogen form a heteroaryl or heterocyclyl group,

with the proviso that the heteroaryl or heterocyclyl of variable R2 is furyl, pyridyl or thiazole only;

(c) R3 is from one to four groups independently selected from the group consisting of: hydrogen, halo, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, C1-8 alkoxy, cyano, C1-4 carboalkoxy, trifluoromethyl, C1-8 alkylsulfonyl, halogen, nitro, hydroxy, trifluoromethoxy, C1-8 carboxylate, aryl, —NR11R12,
wherein;
R11 and R12 are independently selected from H, C1-8 straight or branched chain alkyl, arylalkyl, C3-7 cycloalkyl, carboxyalkyl, aryl or —NR13COR14,

wherein;
R13 is selected from hydrogen or alkyl and R14 is selected from hydrogen, alkyl, substituted alkyl, C1-3 alkoxyl, carboxyalkyl, aryl, arylalkyl, R15R16N (CH2)p—, or R15R16NCO (CH2)p—,

wherein;
R15 and R16 are independently selected from H, OH, alkyl, and alkoxy, and p is an integer from 1-6,

wherein the alkyl group may be substituted with carboxyl, alkyl, aryl, substituted aryl, hydroxamic acid, sulfonamide, sulfonyl, hydroxy, thiol, alkoxy or arylalkyl,

(d) X is selected from C═S, C═O; CH2, CHOH, CHOR19; or CHNR20R21 where R19, R20, and R21 are selected from optionally substituted C1-8 straight of branched chain alkyl, wherein the substituents on the alkyl group are selected from C1-8 alkoxy, hydroxy, halogen, amino, cyano, or NR22R23 wherein R22 and R23 are independently selected from the group consisting of hydrogen, C1-8 straight or branched chain alkyl, C3-7 cycloalkyl, benzyl or aryl;
with the proviso that in a compound of Formula II when R1 is a cyano, then R2 is not phenyl.

2. The compound of claim 1, Formula II, wherein R2 is aryl or heteroaryl.

3. The compound of claim 1, wherein R2 is furyl or substituted furyl.

4. The compound of claim 1, wherein R1 is COOR5, wherein R5 is selected from optionally substituted C1-8 straight or branched chain alkyl.

5. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier.