# Patent

## Title
Peroxidase produced by plant cell cultures

## Abstract

A modified form of beetle luciferase, which has been engineered for improved genetic reporting, is disclosed. The modified form contains one or more new features. Chief among these is removal of the peroxisomal translocation sequence to yield a cytoplasmic form of the enzyme. Other changes include removal of potentially interfering restriction sites and genetic regulatory sites from the gene, improvement of the codon usage for mammalian cells. The modified luciferase reporter enzyme is also devoid of potential N-glycosylation targets to minimize post-translational modification and remains in the cytoplasm of host cells to optimize substrate availability.

## Background
The present invention relates to genetic reporters. Specifically, the present invention is directed to a reporter system utilizing a modified form of beetle luciferase and the gene encoding the modified luciferase. The present invention is also directed to a method of producing the gene encoding the novel modified luciferase.

A full bibliographic citation of the references cited in this application can be found in the section preceding the nucleotide Sequence Listings.

Bioluminescence is the light produced in certain organisms as a result of luciferase-mediated oxidation reactions. The luciferase genes, e.g., the genes from luminous beetle and, in particular, the luciferase from Photinus pyralis (the common firefly of North America), are currently the most popular luminescent reporter genes. Reference is made to Bronstein, et al. (1994) for a review of luminescent reporter gene assays.

Firefly luciferase has become highly valuable as a genetic reporter due to the convenience, sensitivity and linear range of the luminescence assay. Today, luciferase is used in virtually every experimental biological system, including prokaryotic and eukaryotic cell culture, transgenic plants and animals, and cell-free expression systems.

Although referred to simply as firefly luciferase, the enzyme is derived from a specific North American beetle, Photinus pyralis. The enzyme is a monomeric protein (62 kDa) which generates light through monooxygenation of beetle luciferin utilizing ATP and O.sub.2.

The gene encoding luciferase was cloned from Photinus pyralis, and demonstrated to produce active enzyme in E. coli (de Wet, et al., 1987). The cDNA encoding luciferase (luc) continues to gain favor as the gene of choice for reporting genetic activity in animal, plant and microbial cells. The luciferase reaction, modified by the addition of CoA to produce persistent light emission, provides an extremely sensitive and rapid in vitro assay for quantifying luciferase expression in small samples of transfected cells or tissues.

Normally, in the firefly light organ, luciferase is located in specialized peroxisomes of the photocytic cells. When expressed in foreign hosts, a conserved translocation signal within the enzyme structure cause it to accumulate in peroxisomes and glycosomes. In moderate to high levels of expression, the peroxisomes typically become saturated with luciferase, and much of the reporter is found in the cytoplasm (Keller, G. A. et al, 1987). This creates the undesirable circumstance of having the reporter present in two different subcellular compartments, each having potentially different physiological characteristics.

To use luciferase as a genetic reporter, extracts of cells expressing luciferase are mixed with substrates (beetle luciferin, Mg.sup.2+ ATP, and O.sub.2), and luminescence is measured immediately. The assay is very rapid and sensitive, providing gene expression data with little effort. The conventional luciferase assay has been further improved by including coenzyme A in the assay reagent to yield greater enzyme turnover and thus greater luminescence intensity (Promega Luciferase Assay Reagent, Cat.# El500, Promega Corporation, Madison, Wis.). Using this reagent, luciferase activity can be readily measured in luminometers or scintillation counters without the need of a reagent injection device. Luciferase activity can also be detected in living cells by adding luciferin to the growth medium. This in vivo luminescence relies on the ability of beetle luciferin to diffuse through cellular and peroxisomal membranes and on the intracellular availability of ATP and O.sub.2 in the cytosol and peroxisome.

Despite its utility as a reporter, however, luciferase has naturally evolved for the nocturnal mating behavior of beetles and not for the convenience of experimental molecular biologists. Thus, it is not necessarily optimized for the wide variety of host organisms in which it is presently being used.

Further, the growing interest in using luciferase as a real-time, i.e., in vivo, reporter of gene expression in eukaryotic cells has raised concerns that i) the activity of luciferase, which is sequestered in peroxisomes, may suffer from limited substrate availability, and ii) high-level accumulation of luciferase in peroxisomes may adversely affect the physiological well-being of the host cell.

The major limitation may be that luciferase is a peroxisomal enzyme which may impact cellular physiology in some hosts. Localization to the peroxisomes can interfere with normal cellular physiology in two ways. First, large amounts of a foreign protein in the peroxisomes could impair their normal function. Second, many other peroxisomal proteins utilize the same translocation signals (Gould, S. J. et al. 1989), and thus, saturation with luciferase import implies competition for the import of other peroxisomal proteins.

Peroxisomal location of luciferase may also interfere with the performance of the genetic reporter. For instance, the luciferase accumulation in the cell could be differentially affected if it is distributed into two different subcellular compartments. The stability of luciferase in peroxisomes is not known, but may well be different than its stability in the cytosol. If so, the apparent expression of luciferase could be affected by changes in the distribution of luciferase between peroxisomes and the cytosol. Measurements of in vivo luminescence could also be affected since the availability of ATP, O.sub.2, and luciferin within peroxisomes is not known. In particular, the concentration of luciferin could be limited by the need to diffuse across both the cytoplasmic and peroxisomal membranes. This partitioning of luciferase between different intracellular compartments may add unforeseen variability when comparing luciferase activities derived from genetic expression elements with different transcriptional activities.

To create a genetic reporter more generally suitable and convenient for diverse applications, the present invention is directed to mutated forms of the luciferase cDNA (luc; referred to as the "luciferase gene"). The mutated forms of luc, designated luc+ and luc+NF, contain multiple new features. Chief among these is removal of the peroxisomal translocation sequence to yield a cytoplasmic form of the enzyme. Other changes include removal of potentially genetic regulatory sites and inconvenient endonuclease restriction sites from within the gene, and improvement of the codon usage for mammalian and plant cells. The modified luciferase reporter enzyme is also devoid of two potential N-glycosylation targets, thus minimizing unpredictable structural modifications due to post-translational glycosylation. A related gene, luc+NF, has also been developed to allow optimal production of N-terminal fusion proteins.

The present invention is specifically directed to a luciferase gene encoding a modified form of the luciferase of the Photinus pyralis, wherein at least one of the following changes appear: beginning at nucleotide 40 from TTC TAT CCT CTA GAG GAT GGA A to TTC TAT CCG CTG GAA GAT GGA A. beginning at nucleotide 945 from GGG CGC ACC TCT TTC GAA A to TGG CGC TCC CCT CTC TAA G; nucleotide 1302 from AGT TGA CCG CTT GAA GTC TTT AAT TAA ATA C to CGT TGA CCG GAA GTC TCT GAT TAA GTA C; nucleotide 48 from TC TAG A to GC TGG A, wherein such change is conducted without affecting the amino acid sequence; nucleotide 583 from ATA ATG AAT TTC to ATC ATG AAC TCC, wherein such change is conducted without affecting the amino acid sequence; nucleotide 373 from GTA GTG TTT GTT to GTG GTG TTC GTT, wherein such change is conducted without affecting the amino acid sequence; nucleotide 608 from GGT TAC CTA to GTC TGC CTA, wherein such change is conducted without affecting the amino acid sequence; nucleotide 1333 from AAA GGA TAT CAG GTG GCC to AAA GGC TAT CAG GTG GCT, wherein such change is conducted without affecting the amino acid sequence; nucleotide 1365 from ATC GAT ATT GTT A to ATC CAT CTT GCT C, wherein such change is conducted without affecting the amino acid sequence; sequence at nucleotide 646 from TGC GTC AG to TGC CGT AG, wherein such change is conducted without affecting the amino acid sequence; sequence at nucleotide 1158 from GAG AGG CGA ATT ATG TGT CAG AGG A to AAG AGG CGA ACT GTG TGT GAG AGG T, wherein such change is conducted without affecting the amino acid sequence; DNA sequence at nucleotide 1400 from CGG GCG TGG C to CAG GTG TCG C, wherein such change is conducted without affecting the amino acid sequence; replacing asparagine with aspartate; replacing the tripeptide codon sequence AAC ATC ACG with GAC ATC ACT, such that the amino acid sequence asparagine-isoleucine-threonine is replaced with aspartate-isoleucine-threonine; replacing asparagine with glycine; and replacing the tripeptide codon sequence AAC ATT TCG with GGC ATT TCG, such that the amino acid sequence asparagine-isoleucine-serine is replaced with glycine-isoleucine-serine.

Additionally, the present invention is directed to a recombinant DNA sequence containing the luciferase gene described above, and to a host cell containing the recombinant DNA.

The present invention is also directed to a luciferase gene encoding a modified form of the luciferase of Photinus pyralis, wherein at least one of the following changes appear: C-terminal end is replaced by the tripeptide glycine-lysine-threonine; or replaced by isoleucine-alanine-valine.

The present invention is also directed to a modified luciferase gene having the following nucleotide sequence and amino acid sequence as illustrated in Sequence Listings SEQ ID: No. 3 and SEQ. ID: No. 4, respectively, and a protein sequence encoded by the modified luciferase gene.

The present invention is further directed to a modified luciferase gene having the following nucleotide sequence as illustrated in Sequence Listing SEQ ID: No. 3 and a protein sequence encoded by modified luciferase gene.

The present invention is also directed to a process for increasing the expression of a luciferase gene, comprising removing the peroxisomal translocation sequence in the nucleotide sequence to yield a cytoplasmic form of the enzyme.

The present invention is also directed to a process for eliminating the influence of peroxisomes on the expression of a luciferase gene, comprising removing the peroxisomal translocation sequence by mutating the nucleotide sequence to yield a cytoplasmic form of the enzyme.

The primary advantage conferred by this invention is the availability of a modified luciferase reporter gene that is superior in performance and usefulness to currently used cDNA sequences coding beetle luciferase. The present invention will set a new standard for eukaryotic and prokaryotic reporter technology.

A summary of the major benefits of this invention are as follows: cells; firefly luciferase reporter genes in many eukaryotic cell types; regulatory elements or palindromic RNA sequences; and research applications.

FIG. 1 shows a genetic map of a recombinant plasmid pGL2-Control.

FIG. 2 shows a genetic map of a recombinant plasmid pGL2X constructed by introducing an Xba I restriction site at the immediate 3' end of the luc cDNA contained in the pGL2-Control Vector.

FIG. 3 shows a genetic map of a recombinant plasmid pSx tac) Ppy.

FIG. 4 is a chart illustrating the results of Example 1.

FIG. 5 is a chart illustrating the enhanced in vivo luminescence resulting from the changes in the plasmid pGL2X(luc:dX+IAV) in Example 2.

FIG. 6 is a chart illustrating the enhanced luminescence of plasmid pGL2X(luc:KNB+dX+IAV) over unmodified luciferase in Example 3.

Definitions

The following definition is provided to assist in providing a clear and consistent understanding of the scope and detail of the terms:

Amino Acids:

Amino acids are shown either by three letter or one letter abbreviations as follows: Abbreviated Designation Amino Acid ______________________________________ A         Ala          Alanine C         Cys          Cysteine D         Asp          Aspartic acid E         Glu          Glutamic acid F         Phe          Phenylalanine G         Gly          Glycine H         His          Histidine I         Ile          Isoleucine K         Lys          Lysine L         Leu          Leucine M         Met          Methionine N         Asn          Asparagine P         Pro          Proline Q         Gln          Glutamine R         Arg          Arginine S         Ser          Serine T         Thr          Threonine V         Val          Valine W         Trp          Tryptophan Y         Tyr          Tyrosine ______________________________________

Beetle Luciferase

The present invention is directed to a luciferase gene which encodes a modified form of beetle luciferase. For purposes of this invention, beetle luciferase includes, but is not limited to, any luciferase from a luminous beetle, including beetles of the families: Elateridae (click beetles), Phengodidae (glow worms), and Lampyridae (fireflies). All of these beetles produce light by oxidation of beetle luciferin, and all contain a translocation signal for peroxisomes. Unless otherwise directed in this disclosure, there is no wish to limit the term luciferase to one species or variety of beetle. For purposes of explanation and example, reference will be primarily directed to luciferase from the North American firefly (Photinus pyralis).

Unmodified Luciferase Enzyme

To improve the general suitability of luciferase as a genetic reporter, a modified form of the luciferase gene from the common North American firefly Photinus pyralis has been developed. The luciferase gene derived from Photinus pyralis has been extensively studied (see de Wet, et al., 1987). Firefly luciferase has an apparent molecular weight of 62 kilodaltons (kD) and requires luciferin, ATP, and O.sub.2 as substrates.

The nucleotide sequence of luc, and amino acid sequence of the encoded luciferase, are shown in Sequence Listings SEQ ID: No. 1 and SEQ ID: No. 2, which are also found in de Wet, et al. (1987). Amino acids in the native luciferase are numbered starting with "1" at the initiating methionine (de Wet, et al., 1987). Nucleotides in the luciferase gene are numbered starting with "1" at the "A" of ATG(Met).

Modifications to luc

The purpose of the modifications to luc was to minimize potential biological interferences that may complicate the interpretation of reporter data. The strategy was to develop an optimal cytoplasmic form of the luciferase gene by making one or more alterations to the luc gene structure as follows:

Removal of tripeptide sequence "serine-lysine-leucine: The present invention is directed to the ability to increase reporter signal strength and eliminate the influence of peroxisomal physiology by removing the C-terminal amino acids. The amino acid sequence of the native luciferase protein contains the C-terminal tripeptide "serine-lysine-leucine" (-Ser-Lys-Leu), which is well established to be a peroxisome targeting sequence (Keller, et al., 1987, infra.). Removal of this sequence abolishes import into peroxisomes and therefore peroxisomal targeting (Gould, S. J. et al., 1989). However, the effect of removing this sequence on luciferase expression or the specific activity of this modified luciferase has not been determined. This modification alters the structure of both the gene and enzyme.

All mutations to the luciferase cDNA of Photinus pyralis were performed using oligonucleotide-mediated, site specific mutagenesis directed against single-stranded plasmid templates according to the method of Lewis and Thompson (1990). According to this method, a mutagenic primer designed to correct a defective ampicillin resistance gene is used in combination with one or more primers designed to mutate discreet regions within the target gene. Rescued antibiotic resistance coupled with distant non-selectable mutations in the target gene results in high frequency capture of the desired mutations.

Plasmid pGL2X (FIG. 2) was constructed by introducing an Xba I restriction site at the immediate 3' end of the luc cDNA contained within the eucaryotic expression plasmid pGL2-Control (FIG. 1) (Promega Corp., Madison, Wis.). This was accomplished by first subcloning the 2.3 Kb DNA fragment generated by EcoR I/Sal I restriction of pGL2-Control into complementary sites of the prokaryotic luciferase expression vector pSx(tac) Ppy (FIG. 3), performing site-directed mutagenesis to create the desired Xba I site, and then recloning the mutated EcoR I/Sal I restriction fragment back into the original pGL2-Control vector backbone. Plasmid pGL2X was constructed to accept all subsequent mutational variations of the luc gene, and to express the modified luciferase enzymes in transfected mammalian cells.

The luc cDNA contained in pSx(tac) Ppy was mutated to disrupt both an Xba I beginning at bp 48 and a 22 bp imperfect palindrome centered at the Xba I site. The mutated luciferase gene is designated luc:dX. Plasmid pSx(tac) Ppy containing luc:dX provided the target for all subsequent mutagenic reactions directed downstream of the internal Nar I site, thus allowing for the generation of cumulative mutations within the luciferase gene. Intermediate forms of luciferase mutants contained in pSx(tac) Ppy were easily transferred into plasmid pGL2X for expression analysis in mammalian cells by complementary subcloning of the 1.6 Kb luciferase DNA generated by Nar I/Xba I digestion.

Two independent strategies were used to remove the peroxisomal targeting sequence from luciferase. One strategy relied on random mutagenesis to randomly alter the last three amino acids of luciferase, followed by screening for clones exhibiting efficient luminescence. The other strategy relied on deliberate design to achieve a preconceived sequence known to be ineffective for peroxisomal targeting. The result of these two approaches was two structurally distinct protein termini (both by amino acid identities and by position within the three dimensional structure of the protein) which are related only by their lack of targeting activity.

Random Mutagenesis: In the strategy using random mutagenesis, an oligonucleotide was generated to randomly alter the DNA sequence encoding the peroxisome targeting sequence, Ser-Lys-Leu, at the C-terminus of luciferase. pSx(tac) Ppy DNA containing the population of random C-terminal mutations was used to transform E. coli JM109 cells. Ampicillin resistant colonies were screen for bright in vivo luminescence according to the method of Wood, et al. (1987). C-terminal DNA sequences of clones selected for high level luciferase expression were further analyzed for amino acid sequence deviation from the natural peroxisome targeting sequence. A mutant luciferase containing the C-terminal tripeptide Ile-Ala-Val was selected by this method and, combined with the modification to remove the palindrome, was designated luc:dX+IAV.

Designed Mutagenesis: An independent strategy employing sequence-specific mutagenesis was used to generate a second luciferase variant containing the sequence Gly-Lys-Thr in substitution of the natural peroxisome targeting sequence. This luciferase variant, combined with the modification to remove the palindrome, was designated luc:dX+GKT. The tripeptide Gly-Lys-Thr was generated by rational design based on investigation by Sommer, et al., (1992) on the affect of C-terminal amino acid composition on the import of luciferase into glycosomes of Trypanosoma brucei. Glycosomes are membrane bound organelles specialized for energy production through glycolytic processes. Thus, glycosomes are functionally different from peroxisomes. However, the signals directing intracellular trafficking of proteins to glycosomes are similar to, though seemingly less stringent than, signals that mediate the targeting of proteins to peroxisomes. The luc:dX+IAV and luc:dX+GKT variants were cloned into the Nar I/Xba I sites of pGL2X to generate plasmids pGL2X(luc:dX+IAV) and pGL2X(luc:dX+GKT). DNA and amino acid changes specific to the major internal palindrome site and C-terminus of these three pGL2 derivatives and pGL2X(luc+) are displayed in Table 1. In addition to the changes described in Table 1, pGL2X(luc+) embodies all DNA and amino acid changes listed in Table 2. The nucleotide sequence of luc+ and the amino acid sequence of the encoded luciferase are shown in Sequence Listings SEQ ID: No. 3 and SEQ ID: No. 4, respectively. __________________________________________________________________________ Luciferase Plasmid luc Palindrome Region      C-Terminal Sequence    Localization __________________________________________________________________________ Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Ser Lys Leu Stop pGL2   GC G CCA  TTCT AT CCT CTIA GAG GATG GA ACC  GCT GGA AAG TCC AAA TTG TAA Peroxisomes #34         Xba I          #1,636 Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Ser Lys Leu Stop pGL2x  GC G CCA  TTCT AT CCT CTIA GAG GATG GA ACC  GCT GGA AAG TCC AAA T TC TAG NAT #34         Xba I          #1,636     Xba I pGL2X  Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Ser Lys Leu Stop (luc:dX) GCG CCA TTC TAT CCG CTG GAA GAT GGA ACC GCT GGA AAG TCC AAA TTG TAA T TC TAG Peroxisomes #34                        #1,636       Xba I pGL2X  Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Thr Stop (luc:dX+GKT) GCG CCA TTC TAT CCG CTG GAA GAT GGA ACC GCT GGA AAG AAC TAA T TC TAG Cytosol #34                        #1,636      Xba I pGL2X  Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Ile Ala Val Stop (luc:dX+IAV) GCG CCA TTC TAT CCG CTG GAA GAT GGA ACC GCT GGA AAG ATC GCC GTG TAA T TC TAG Cytosol #34                        #1,636        Xba I pGL2X  Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly Lys Ile Ala Val Stop (luc+) GCG CCA TTC TAT CCG CTG GAA GAT GGA ACC GCT GGA AAG ATC GCC GTG TAA T TC TAG Cytosol #34                        #1,636        Xba I __________________________________________________________________________

Other modifications: In addition to removing the peroxisomal translocation signal, several other modifications were made to enhance the reliability and convenience of luciferase as a genetic reporter. These modifications were made using the same methods as described above for the sequence specific downstream of the internal Nar I site.

Table 2 (below) presents the complete list of sequence modifications embodied in luc+, which can be divided into the following classes: restriction sites for Xba I, EcoR I, BstE II, EcoR V, and Cla I. To facilitate subcloning of the gene into diverse genetic constructs, these sites were removed by changing nucleotides in the DNA sequence without affecting the amino acid sequence.

An Nco I site was added at the initiating methionine codon (ATG) to aid in subcloning into many vectors which contain this site. The Nco I site may also be used to create N-terminal fusion proteins with luciferase. However, for this purpose using luc+NF (see below) is recommended.

To generate the Nco I site, the same site-specific mutagenesis methods described above were used. The 0.62 Kb Hind IIIcoR I fragment of pGL2-Control Vector was subcloned into the pSx(tac) Ppy linearized with Hind III and EcoR I. Following mutagenesis, the mutated Hind III/EcoR I fragment was subcloned into pGL2X(luc:dX+IAV). embedded within its coding region which could mediate genetic activity either through the gene's native regulatory function or as a consequence of spurious recognition by transcription factors in a foreign host. In either case, these sequences would interfere with the "genetically neutral" behavior expected of a reporter gene.

To minimize this possibility, the luciferase gene sequence was scanned using a database of consensus sequences for transcription factor binding sites (Faisst and Meyer, 1992). Many sites which could potentially interact with common factors were removed. In some cases where it was convenient in the modification strategy, less common potential regulatory sites were also removed. As with the removal of restriction sites, the potential regulatory sites were removed through changes in the DNA sequence that do not affect the encoded amino acid sequence. palindromic sequences which could spuriously affect expression (22bp, 18bp, and 16bp, each with one mismatch) were also removed. The physiological significance of these sequences is unknown, however, they present potential regions of mRNA secondary structure which can affect translational efficiency. Disrupting these palindrome sequences increased reporter signal strength. This modification affects the structure of the gene only, not the amino acid strength. codon usage presumably reflects the availability of tRNA isoforms in different organisms. Efficiently expressed genes utilize the most abundant tRNA isoforms. Codon usage in mammalian cells reveals a preference for cytosine (C) or guanine (G) in the third codon position; many codons containing adenine (A) or thymidine (T) occur rarely.

However, beetle luciferases generally have a high A/T content, biasing the codon frequency significantly from that of mammals. To achieve a codon usage in the firefly luciferase gene that is more congruent with mammalian genetics, the sequence modifications described above were designed wherever possible to yield more common codons (Wada, K. et al., 1992).

Also, where possible in the modifications strategy, the codons were changed from ATA to ATC (Ile), GTA to GTG (Val), and TTA to CTG or CTC (Leu). These changes were chosen because they convert particularly infrequent codons to ones which are highly frequent. In total, of the 69 codons that were modified to create luc+, 54 represent more common codons in mammalian cells. The average usage frequency of all modified codons increases from 14.5 in luc to 25.7 in luc+ (usage frequency is the occurrence per 1000 codons). the cytosol normally does not contain any post-translational modifications. However, gene fusions may be made of luc+ (or luc+NF) which are intended to direct a hybrid protein into the endoplasmic reticulum or Golgi apparatus. In these cellular compartments, N-linked glycosylation is known to occur at -Asn-X-(Ser/Thr)--sequences, which would have an unknown effect on luciferase enzymatic activity. To prevent the potential occurrence of N-linked glycosylation, two consensus glycosylation sites within the luciferase sequence were altered. The modified enzyme does not exhibit any apparent change to its chemical activity. A third consensus glycosylation site is highly conserved among beetle luciferases and could not be altered in firefly luciferase without affecting enzyme performance. Most likely this site is not near the protein surface, possibly making it inaccessible for glycosylation. common enzyme and gene. The modifications leading to greater reporter signal strength described above act through largely independent mechanisms, and thus their effects should be cumulative. Reporter signal strength is increased by combining the above described modifications into a common enzyme and associated gene structure to gain a cumulative enhancement. __________________________________________________________________________ Summary of Luciferase Gene Modifications in luc+ Purpose of Modification Sequence Modification in luc+ __________________________________________________________________________ Met.sub.1 Glu.sub.2 Introduce Nco I site for the luc: AAAATGGAA construction of N-terminal Met.sub.1 Glu.sub.2 fusions with luc+. luc+ T CCATGGAA Nco I #47 Xba I Remove internal Xba I site; luc: C TCTAGAGG disrupt extended palindrome. luc+: CGCTGGAAG Asn.sub.50 Ile.sub.51 Thr.sub.52 Eliminate potential glycosylation luc: AACATCACGTACGCGGAA and ATF sites.    Asp.sub.50 Ile.sub.51 Thr.sub.52 luc+: GACATCACTTACGCTGAG Asn.sub.119 Ile.sub.120 Ser.sub.121 Eliminate potential luc: AACATTTCG glycosylation site. Gly.sub.119 Ile.sub.120 Ser.sub.121 luc+: GGCATTTCG #373 Remove potential TGT-3 site; luc: GTAGTGTTTGTT improve codon usage. luc+: GTGGTGTTCGTT #426 Improve codon usage. luc: ATTACCAATAATCCAG luc+: GCTCCCAATCATCCAA #546 Improve codon usage. luc: ACCAGAGTCCTTTGATCGTGACAAA luc+: GCCAGAGTCCTTCGATAGGGACAAG #583  EcoR I Remove internal EcoR I site, luc: ATAAT GAATTCC improve codon usage. luc+: ATCATGAACTCC #608 BstE II Remove internal BstE II site and luc: GGTTACCTAAGGGTGTGGCCCTTCCG potential AP2 and LF-A1 sites; luc+: GTCTGCCTAAAGGTGTCGCTCTGCCT improve codon usage. #646 Remove potential AP1 site. luc: TGCGTCAG luc+: TGCGTGAG #820 Improve codon usage. luc: TTACGATCCCTTCAGGATTACAAA luc+: CTGAGGAGCCTTCAGGATTACAAG #856 Improve codon usage. luc: TTGCTAGTACCAACCCTATTTTCA luc+: CTGCTGGTGCCAACCCTATTCTCC #945 Eliminate internal palindrome; luc: GGGCGCACCTCTTTCGAAA improve codon usage. luc+: TGGCGCTCCCCTCTCTAAG #984 Improve codon usage. luc: AAAACGCTTCCATCTTCCAGGGATACGA luc+: CAAGAGGTTCCATCTGCCAGGTATCAGG #1158 Eliminate potential AP1 site; luc: GAGAGGCGAATTATGTGTCAGAGGA improve codon usage. luc+: AAGAGGCGAACTGTGTGTGAGAGGT #1302 Eliminate palindrome structure; luc: AGTTGACCGCTTGAAGTCTTTAATTAAATAC improve codon usage. luc+: CGTTGACCGCCTGAAGTCTCTGATTAAGTAC #1333  EcoR V Remove internal EcoR V site; luc: AAAG GATATCAGGTGGCC improve codon usage. luc+: AAAGGCTATCAGGTGGCT #1365 Cla I Remove internal Cla I site; luc: ATCGATATTGTTA improve codon usage. luc+: ATCCATCTTGCTC #1400 Remove potential Spl luc: CGGGCGTGGC and AP2 sites. luc+: CAGGTGTCGC Gly.sub.546 Lys.sub.547 Ser.sub.548 Lys.sub.549 Leu.sub.550 stop Remove peroxisome targeting luc: GGAAAGTCCAAATTGTAA sequence.         Gly.sub.546 Lys.sub.547 Ile.sub.548 Ala.sub.549 Val.sub.550 stop luc+: GGAAAGATCGCCGTGTAA __________________________________________________________________________ The numbering scheme for amino acids is relative to the first amino acid, Met #1, of luciferase. The numbering of nucleotides is relative to the first base, "A", of the luciferase open reading frame.

N-terminal fusions with luciferase: luc+NF:

A variation of luc+, designated luc+NF, was designed for easier construction of N-terminal fusions (NF) with luciferase. The nucleotide sequence of luc+NF and the amino acid sequence of the encoded luciferase are shown in the Sequence Listings SEQ ID: No. 5 and SEQ ID: No. 6, respectively.

The luc+NF gene contains a unique BstE II restriction site located immediately downstream of the luciferase translational initiation codon (ATG). This site was generated by the same site-specific mutagenesis methods described above, yielding the sequence CC ATG GTC ACC GAC GCC from AA ATG GAA GAC GCC. The necessary manipulations to modify upstream of the Nar I site were the same as described above for generating luc+. Following mutagenesis, the Hind IIIcoR I fragment was subcloned into pGL2X(luc:dX+IAV) to yield pGL2X(luc:KNB+dX+IAV).

The BstE II site allows construction of N-terminal fusions that replace the original ATG codon by the newly introduced DNA; sequences also may be placed between the luciferase gene and its initiation codon. By removing the ATG codon from the fusion site, spurious internal initiation at this codon can be confidently avoided, preventing the possibility of coexpressing full-length, unfused luciferase. Internal initiation from the next available ATG codon generates a polypeptide which is too short to support luminescence.

Concomitant with placement of the BstE II site in luc+NF is the generation of two new amino acids at positions 2 and 3 of the modified luciferase enzyme. The altered N-terminal amino acid sequence of luc+NF, illustrated in Sequence Listing SEQ ID: No. 6, reduces luciferase expression 4 to 5-fold relative to that produced by the luc+ construct. Therefore, luc+NF is recommended specifically for the construction of N-terminal fusion proteins devoid of an internal ATG codon at the luciferase juncture, or that require the resident ATG for translational initiation.

New cassette vectors: pSP-luc+ and pSP-luc+NF:

pSP-luc+ and pSP-luc+NF are cassette plasmids containing the improved firefly luciferase genes, luc+ and luc+NF. These cassette vectors are not themselves intended for the eukaryotic expression of luciferase because they do not contain eukaryotic genetic regulatory elements. The luc+ and luc+NF gene are positioned downstream of an SP6 promoter and minimal ribosome binding site which, in the presence of SP6 polymerase, drive in vivo and in vitro expression of the modified luciferases. An opposing T7 promoter is also located immediately downstream of luc+ and luc+NF. These promoters allow for the convenient synthesis of sense and anti-sense luc+ or luc+NF transcripts for studies involving in situ hybridization, RNA processing, RNA transfection, or coupled in vitro transcription/translation and protein folding. Multiple cloning sites containing recognition sequences for a number of commonly used restriction enzymes are positioned 5' and 3' of luc+ and luc+NF.

The following examples are presented to illustrate the advantages of the present invention and to assist one of ordinary skill in making and using the same. The examples are not intended in any way to otherwise limit the scope of the disclosure or protection granted by the patent.

A major concern in the use of the native firefly luciferase as a genetic reporter is its intracellular partitioning into peroxisomes. The presence of this foreign protein in peroxisomes, and moreover, the resulting competition with native host proteins for peroxisomal transport has undefined affects on the normal cellular physiology. Variable subcellular localization of luciferase also compromises its value as a quantitative marker of gene activity. These potential problems reduce the general reliability of luciferase in reporter applications.

In the present invention, modified forms of luciferase were created which have the peroxisomal targeting sequence removed, or otherwise inactivated. The specific structural changes of these modifications are shown in Table 2. Expression of these modified luciferases in eukaryotic cells yields greater luminescence. Since the increased expression is evident for luciferases containing structurally independent modifications, the improved reporter activity is due to the inactivation of the peroxisomal targeting sequence and not to structural nuances of the modifications. Other modification of the luciferases revealed that eliminating a palindrome sequence from the encoding gene also yielded greater expression of the reporter.

Expression of Modified Luciferases in Mammalian Cells

Plasmids expressing the non-mutated luc cDNA and three mutant variations were introduced into NIH3T3 cells to determine relative luciferase activities present in their prepared cell extracts. The plasmids tested were i) pGL2-Control containing the non-mutated luc gene, ii) pGL2X(luc:dX) expressing luc mutated to disrupt the major palindrome sequence centered between base pair 51 and 52, iii) pGL2X(luc:dX+IAV) containing luc mutated to disrupt the major palindrome sequence and to substitute the native Ser-Lys-Leu C-terminal tripeptide for Ile-Ala-Val and iv), pGL2X(luc:dX+GKT) containing luc mutated to disrupt the major palindrome sequence and to substitute the native C-terminal tripeptide for Gly-Lys-Thr.

Plasmid DNA's were delivered into cultured mammalian cells using a modified calcium phosphate-mediated transfection procedure. NIH3T3 cells were cultured in 75 cm.sup.2 polystyrene culture flasks containing 25 ml of DMEM+FCS medium (Dulbecco's Modified Eagles Medium supplemented with 10% calf serum). Culture flasks containing cells were incubated in a 37.degree. C./5% CO.sub.2 environment until approximately 80% confluence was observed. Growth medium was removed and the cell monolayer was covered with 5 ml of Trypsin-EDTA solution diluted ten-fold with Hank's Balanced Salts solution. The trypsin solution was aspirated 30 seconds after addition and cells were allowed to incubate for 2 minutes at 37.degree. C. Cells were harvested by rinsing the culture flask with of 10 ml of medium. Cell titer was determined and 3.0.times.10.sup.6 cells were transferred to a 50 ml, sterile, screw-cap tube containing sufficient medium to obtain a final cell suspension volume of 33 ml.

In separate preparations, 1.8 ml of 250 mM CaCl.sub.2 containing 15 .mu.g of plasmid pCAT-Control (Promega Corp., Madison, Wis.) and 30 .mu.g of either plasmid pGL2-Control, plasmid pGL2X(luc:dX), plasmid pGL2X(luc:dX+IAV), or plasmid pGL2X(luc:dX+GKT) were added to 1.8 ml of 2.times. HEPES buffer. The resulting 3.6 ml volumes of colloidal DNA/calcium phosphate were added to the prepared 33 ml volumes of cell suspension. The combined suspensions were rapidly mixed and 12 ml aliquots were immediately dispensed into each of three 100 cm.sup.2 round polystyrene culture plates. Each plate contained the equivalent of 1.times.10.sup.6 cells, 5 .mu.g of control plasmid encoding CAT and 10 .mu.g of an experimental plasmid encoding a luciferase variant. Transfected cells were incubated in a 37.degree. C./5% CO.sub.2 environment for 20 hr prior to providing the cells with fresh medium.

At 30 hours post-transfection each culture was harvested by removing growth medium, washing the adherent cells once with 10 ml phosphate buffered saline (PBS; 137 mM NaCl, 2.68 mM KCl, 1.47 mM KH.sub.2 PO.sub.4, 8.1 mM Na.sub.2 HPO.sub.4 ; pH 7.4), adding 1 ml Reporter Lysis Buffer (RLB) and scraping vigorously with a plastic cell lifter. Lysates were transferred to 1.5 ml microfuge tubes and cleared of cellular debris by spinning at 14,000 rpm in a refrigerated microfuge. Luciferase activities and chloramphenicol acetyl transferase (CAT) activities were quantified as described in Promega's Technical Bulletins #101 and #084, respectively (Promega Corporation, Madison, Wis.). CAT activities determined for individual cultures within a given experimental set were used as internal standards, and provided a means of normalizing the determined experimental luciferase activities.

All cell culture grade medium components, Trypsin-EDTA and salt solutions were obtained from GibcoBRL (Gaithersburg, Md.). All plastic ware was obtained from Corning (Corning, N.Y.). CaCl.sub.2, and HEPES buffer solutions are components of the Profection.RTM. Mammalian Transfection System available from Promega Corp. (Madison, Wis.). RLB and all luciferase and CAT assay reagents and protocols are provided as components of the Luciferase Assay System and CAT Enzyme Assay System kits available from Promega Corp. (Madison, Wis.).

The results of these experiments, shown in FIG. 4, reveal that targeting into peroxisomes suppresses luciferase expression. This suppression limits reporter sensitivity and demonstrates modulation of reporter activity by a means other than gene transcription. By removing the effect of peroxisomal targeting, greater expression is achieved and reporter activity is disassociated from peroxisome physiology. This is especially important since prior results show that luciferase often is partitioned into both peroxisomes and the cytosol due to saturation of the translocation mechanism. Changes in expression would alter the balance of this partition, thus modulating the reporter activity. By inactivating the peroxisomal target sequence, the effects of partitioning are eliminated.

The results also reveal that a palindrome in the luciferase gene suppresses expression. By disrupting this palindrome through mutagenesis without altering the encoding of amino acid, greater luciferase expression was achieved.

Expression of luciferase may also be measured from living cells by adding the substrate luciferin to the growth medium. Luminescence is thus emitted from the cells without disrupting their physiology. Experimental results show that the modified luciferase gene yields greater luminescence expression also when measured from living cells.

The level of in vivo luciferase expression was compared between NIH3T3 cells transfected with plasmid pGL2-Control expressing luc cDNA, or plasmid pGL2X(luc:dX+IAV) expressing luc mutated to disrupt the major palindrome sequence and to substitute the Ser-Lys-Leu peroxisome targeting tripeptide with Ile-Ala-Val. Transfection and preparation of NIH3T3 cells were performed as described in Example 1, except that plasmid pCAT-Control was not included. As described, the 3.6 ml volumes of prepared colloidal DNA/calcium phosphate were added directly to the prepared 33 ml volumes of cell suspension and rapidly mixed. 3 ml aliquots were immediately dispensed into each of twelve pre-sterilized, flat-bottom, 28 mm O.D. borosilicate vials containing threaded caps (Fisher Scientific, Pittsburgh, Pa.).

The culture vials, each containing the equivalent of 2.5.times.10.sup.5 cells and 2.5 .mu.g of plasmid DNA, were incubated in a 37.degree. C./5% CO.sub.2 environment for 20 hr prior to providing the culture with 1 ml of fresh medium. At 30 hours post-transfection each culture medium was supplemented with an additional 1 ml of 37.degree. C. medium containing 1.0 mM luciferin, 1% DMSO, and 0.01% Tween-20. In vivo expression of the luciferase reporter gene was determined by placing individual culture vials in the sample chamber of a Turner Designs Model 20e Luminometer (Turner Designs, Sunnyvale, Calif.) and quantifying cellular light emission every 15 seconds over a 3.25 minute period.

Reference is made to FIG. 5, which illustrates enhanced in vivo luminescence resulting from the changes embodied in the plasmid pGL2X(luc:dX+IAV). Cells

Constructs of fused genes that contain an ATG codon at the fusion juncture frequently suffer from some level of internal (i.e., spurious) translational initiation. This phenomenon causes a persistent low-level co-expression of the unfused reporter protein that cannot be easily discriminated from the expression pattern of the desired fusion construct. It is not uncommon to find that the enzymatic activity of a reporter enzyme is diminished by adding to it a fusion partner. Therefore, co-expression of a chimeric reporter enzyme and the natural reporter enzyme will undermine accurate and meaningful interpretation of any experimental results in which enzymatic activity of the fused protein is intended to report in vivo physiological responses.

The Nco I restriction site at the N-terminus of luc+ allows only for the construction N-terminal luciferase fusions which retaining the resident luciferase initiation codon at the fusion juncture. To extend the utility of luc+ in constructing N-terminal fusions, the gene was modified to include a unique BstE II restriction site beginning at nucleotide 2 of the luciferase gene. This gene variation, designated luc+NF, enables the construction of N-terminal fusions in which the resident ATG (Met) of luciferase is either excluded or segregated to the new N-terminus of the fusion protein.

An intermediate in the design of luc+ is luc:KNB+dX+IAV which contains only the modified N-terminal sequence, the disrupted 22 bp palindrome, and the modified C-terminus encoding Ile-Ala-Val. This intermediate was made by the methods described above. Comparative in vitro analysis of expression levels were conducted using NIH3T3 cells transfected with plasmids pGL2-Control, pGL2X(luc:KNB+dX+IAV) and pGL2X(luc:dX+IAV). Expression of luciferase from luc:KNB+dX+IAV is greater than that of the unmodified peroxisomal luciferase encoded by luc. Because the modified N-terminus alone is known to reduce expression efficiency 4 to 5-fold, the increase in expression evident in this experiment is due to elimination of peroxisomal targeting.

FIG. 6 demonstrates that the plasmid pGL2X(luc:KNB+dX+IAV) shows enhanced luminescence over the unmodified, i.e., peroxisomal, luciferase (pGL2). CV-1 Cells

The changes incorporated into luc+ are intended to minimize the potential for unexpected interferences with reporter performance under specific experimental conditions, providing a more reliable indicator of genetic activity than the native luciferase cDNA clone. The specific changes to the structure of the luciferase gene are listed in Table 2, and include the modifications described in Examples 1 through 3. The structure of luc+NF is identical to luc+ except for a modification to the N-terminus allowing for optimal creation of gene fusions. The structures of luc+ and luc+NF represent the optimal compositions of the invention.

In other cell types, differences between the performance of luc+ and luc are apparent. To show this, four mammalian cell lines, NIH3T3, HeLa, CHO, and CV-1, were tested for their level of expressing peroxisomal luciferase encoded by luc, and cytosolic luciferase encoded by luc+. Plasmid pGL2-Control, which expresses the unmodified peroxisomal luciferase enzyme, and plasmid pGL2X(luc+), which expresses the engineered cytosolic luciferase enzyme, were introduced into CHO, NIH3T3, CV-1 and HeLa cells. Cells were prepared and transfected using the calcium phosphate-mediated transfection procedure described in Example 1. Cells were cultured in 75 cm.sup.2 polystyrene culture flasks containing 25 ml of either DMEM+FCS medium or DMEM/F12+FCS medium (a 50:50 combination of Dulbecco's Modified Eagles Medium and Ham F12 Nutrient Mix, further supplemented with 10% calf serum).

The results showed that in cell lines expressing relatively high levels of luciferase activity, the performance of luc+ and luc were comparable (Table 3). With the lower levels of expression, however, luc+ supported relatively higher levels of luciferase. From our earlier experiments in NIH3T3 cells, this greater level of expression is due predominantly to the removal of the peroxisomal translocation signal, suggesting the difference in luciferase expression here is apparently associated with its translocation into peroxisomes.

These results are consistent with the hypothesis that peroxisomal targeting suppresses reporter expression. Since higher levels of expression led to saturation of the peroxisomes, a large portion of the native luciferase synthesized in CHO and CV-1 cells may be in the cytosol. These conditions should minimize differences between the cytoplasmic form of luciferase encoded by luc+ and the native luciferase encoded by luc. Under lower levels of expression, however, the differences between the luciferase forms would become more apparent as they are increasingly segregated into their respective subcellular compartments. ______________________________________ Relative Expression of luc+ and luc in Various Mammalian Cell Lines Relative Luminescence Expression Cell Type luc           luc+     Ratio ______________________________________ NIH3T3    0.0011        0.0050   4.7 HeLa      0.0033        0.0062   1.9 CHO       0.18          0.20     1.1 CV-1      0.90          1.00     1.1 ______________________________________

It is understood that the invention is not confined to the particular construction and arrangements herein illustrated and described, but embraces such modified forms thereof and come within the scope of the claims following the bibliography. Promega Corporation. SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 20 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1650 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1649 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCT48 MetGluAspAlaLysAsnIleLysLysGlyProAlaProPheTyrPro 151015 CTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGA96 LeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLysArg 202530 TACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAG144 TyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIleGlu 354045 GTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCA192 ValAsnIleThrTyrAlaGluTyrPheGluMetSerValArgLeuAla 505560 GAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTA240 GluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleValVal 65707580 TGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTA288 CysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAlaLeu 859095 TTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGT336 PheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGluArg 100105110 GAATTGCTCAACAGTATGAACATTTCGCAGCCTACCGTAGTGTTTGTT384 GluLeuLeuAsnSerMetAsnIleSerGlnProThrValValPheVal 115120125 TCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAATTACCA432 SerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeuPro 130135140 ATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGA480 IleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGlnGly 145150155160 TTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTT528 PheGlnSerMetTyrThrPheValThrSerHisLeuProProGlyPhe 165170175 AATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATT576 AsnGluTyrAspPheValProGluSerPheAspArgAspLysThrIle 180185190 GCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTG624 AlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGlyVal 195200205 GCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGAT672 AlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArgAsp 210215220 CCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTT720 ProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSerVal 225230235240 GTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTG768 ValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyrLeu 245250255 ATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTG816 IleCysGlyPheArgValValLeuMetTyrArgPheGluGluGluLeu 260265270 TTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTA864 PheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeuVal 275280285 CCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATAC912 ProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLysTyr 290295300 GATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCG960 AspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeuSer 305310315320 AAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATA1008 LysGluValGlyGluAlaValAlaLysArgPheHisLeuProGlyIle 325330335 CGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACA1056 ArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIleThr 340345350 CCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTT1104 ProGluGlyAspAspLysProGlyAlaValGlyLysValValProPhe 355360365 TTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTT1152 PheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGlyVal 370375380 AATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGT1200 AsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSerGly 385390395400 TATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGA1248 TyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAspGly 405410415 TGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTC1296 TrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHisPhe 420425430 TTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATATCAG1344 PheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyrGln 435440445 GTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATC1392 ValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsnIle 450455460 TTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTT1440 PheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGluLeu 465470475480 CCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAA1488 ProAlaAlaValValValLeuGluHisGlyLysThrMetThrGluLys 485490495 GAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTG1536 GluIleValAspTyrValAlaSerGlnValThrThrAlaLysLysLeu 500505510 CGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGA1584 ArgGlyGlyValValPheValAspGluValProLysGlyLeuThrGly 515520525 AAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAG1632 LysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLysLys 530535540 GGCGGAAAGTCCAAATTG1650 GlyGlyLysSerLys 545 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 549 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: MetGluAspAlaLysAsnIleLysLysGlyProAlaProPheTyrPro 151015 LeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLysArg 202530 TyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIleGlu 354045 ValAsnIleThrTyrAlaGluTyrPheGluMetSerValArgLeuAla 505560 GluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleValVal 65707580 CysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAlaLeu 859095 PheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGluArg 100105110 GluLeuLeuAsnSerMetAsnIleSerGlnProThrValValPheVal 115120125 SerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeuPro 130135140 IleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGlnGly 145150155160 PheGlnSerMetTyrThrPheValThrSerHisLeuProProGlyPhe 165170175 AsnGluTyrAspPheValProGluSerPheAspArgAspLysThrIle 180185190 AlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGlyVal 195200205 AlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArgAsp 210215220 ProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSerVal 225230235240 ValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyrLeu 245250255 IleCysGlyPheArgValValLeuMetTyrArgPheGluGluGluLeu 260265270 PheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeuVal 275280285 ProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLysTyr 290295300 AspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeuSer 305310315320 LysGluValGlyGluAlaValAlaLysArgPheHisLeuProGlyIle 325330335 ArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIleThr 340345350 ProGluGlyAspAspLysProGlyAlaValGlyLysValValProPhe 355360365 PheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGlyVal 370375380 AsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSerGly 385390395400 TyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAspGly 405410415 TrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHisPhe 420425430 PheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyrGln 435440445 ValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsnIle 450455460 PheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGluLeu 465470475480 ProAlaAlaValValValLeuGluHisGlyLysThrMetThrGluLys 485490495 GluIleValAspTyrValAlaSerGlnValThrThrAlaLysLysLeu 500505510 ArgGlyGlyValValPheValAspGluValProLysGlyLeuThrGly 515520525 LysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLysLys 530535540 GlyGlyLysSerLys 545 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1650 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1651 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCG48 MetGluAspAlaLysAsnIleLysLysGlyProAlaProPheTyrPro 151015 CTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGA96 LeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLysArg 202530 TACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAG144 TyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIleGlu 354045 GTGGACATCACTTACGCTGAGTACTTCGAAATGTCCGTTCGGTTGGCA192 ValAspIleThrTyrAlaGluTyrPheGluMetSerValArgLeuAla 505560 GAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTA240 GluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleValVal 65707580 TGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTA288 CysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAlaLeu 859095 TTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGT336 PheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGluArg 100105110 GAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTT384 GluLeuLeuAsnSerMetGlyIleSerGlnProThrValValPheVal 115120125 TCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAGCTCCCA432 SerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeuPro 130135140 ATCATCCAAAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGA480 IleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGlnGly 145150155160 TTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTT528 PheGlnSerMetTyrThrPheValThrSerHisLeuProProGlyPhe 165170175 AATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGACAAGACAATT576 AsnGluTyrAspPheValProGluSerPheAspArgAspLysThrIle 180185190 GCACTGATCATGAACTCCTCTGGATCTACTGGTCTGCCTAAAGGTGTC624 AlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGlyVal 195200205 GCTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGAGAT672 AlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArgAsp 210215220 CCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTT720 ProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSerVal 225230235240 GTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTG768 ValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyrLeu 245250255 ATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTG816 IleCysGlyPheArgValValLeuMetTyrArgPheGluGluGluLeu 260265270 TTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCTGCTGGTG864 PheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeuVal 275280285 CCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAATAC912 ProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLysTyr 290295300 GATTTATCTAATTTACACGAAATTGCTTCTGGTGGCGCTCCCCTCTCT960 AspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeuSer 305310315320 AAGGAAGTCGGGGAAGCGGTTGCCAAGAGGTTCCATCTGCCAGGTATC1008 LysGluValGlyGluAlaValAlaLysArgPheHisLeuProGlyIle 325330335 AGGCAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACA1056 ArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIleThr 340345350 CCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTT1104 ProGluGlyAspAspLysProGlyAlaValGlyLysValValProPhe 355360365 TTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTT1152 PheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGlyVal 370375380 AATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCCGGT1200 AsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSerGly 385390395400 TATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGA1248 TyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAspGly 405410415 TGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTC1296 TrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHisPhe 420425430 TTCATCGTTGACCGCCTGAAGTCTCTGATTAAGTACAAAGGCTATCAG1344 PheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyrGln 435440445 GTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCAACACCCCAACATC1392 ValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsnIle 450455460 TTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAACTT1440 PheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGluLeu 465470475480 CCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAA1488 ProAlaAlaValValValLeuGluHisGlyLysThrMetThrGluLys 485490495 GAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTG1536 GluIleValAspTyrValAlaSerGlnValThrThrAlaLysLysLeu 500505510 CGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGA1584 ArgGlyGlyValValPheValAspGluValProLysGlyLeuThrGly 515520525 AAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAG1632 LysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLysLys 530535540 GGCGGAAAGATCGCCGTG1650 GlyGlyLysIleAlaVal 545550 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 550 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: MetGluAspAlaLysAsnIleLysLysGlyProAlaProPheTyrPro 151015 LeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLysArg 202530 TyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIleGlu 354045 ValAspIleThrTyrAlaGluTyrPheGluMetSerValArgLeuAla 505560 GluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleValVal 65707580 CysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAlaLeu 859095 PheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGluArg 100105110 GluLeuLeuAsnSerMetGlyIleSerGlnProThrValValPheVal 115120125 SerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeuPro 130135140 IleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGlnGly 145150155160 PheGlnSerMetTyrThrPheValThrSerHisLeuProProGlyPhe 165170175 AsnGluTyrAspPheValProGluSerPheAspArgAspLysThrIle 180185190 AlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGlyVal 195200205 AlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArgAsp 210215220 ProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSerVal 225230235240 ValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyrLeu 245250255 IleCysGlyPheArgValValLeuMetTyrArgPheGluGluGluLeu 260265270 PheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeuVal 275280285 ProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLysTyr 290295300 AspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeuSer 305310315320 LysGluValGlyGluAlaValAlaLysArgPheHisLeuProGlyIle 325330335 ArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIleThr 340345350 ProGluGlyAspAspLysProGlyAlaValGlyLysValValProPhe 355360365 PheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGlyVal 370375380 AsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSerGly 385390395400 TyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAspGly 405410415 TrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHisPhe 420425430 PheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyrGln 435440445 ValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsnIle 450455460 PheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGluLeu 465470475480 ProAlaAlaValValValLeuGluHisGlyLysThrMetThrGluLys 485490495 GluIleValAspTyrValAlaSerGlnValThrThrAlaLysLysLeu 500505510 ArgGlyGlyValValPheValAspGluValProLysGlyLeuThrGly 515520525 LysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLysLys 530535540 GlyGlyLysIleAlaVal 545550 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1653 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1650 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: ATGGTCACCGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTAT48 MetValThrAspAlaLysAsnIleLysLysGlyProAlaProPheTyr 151015 CCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAG96 ProLeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLys 202530 AGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATC144 ArgTyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIle 354045 GAGGTGGACATCACTTACGCTGAGTACTTCGAAATGTCCGTTCGGTTG192 GluValAspIleThrTyrAlaGluTyrPheGluMetSerValArgLeu 505560 GCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTC240 AlaGluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleVal 65707580 GTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCG288 ValCysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAla 859095 TTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAA336 LeuPheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGlu 100105110 CGTGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTC384 ArgGluLeuLeuAsnSerMetGlyIleSerGlnProThrValValPhe 115120125 GTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAGCTC432 ValSerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeu 130135140 CCAATCATCCAAAAAATTATTATCATGGATTCTAAAACGGATTACCAG480 ProIleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGln 145150155160 GGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGT528 GlyPheGlnSerMetTyrThrPheValThrSerHisLeuProProGly 165170175 TTTAATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGACAAGACA576 PheAsnGluTyrAspPheValProGluSerPheAspArgAspLysThr 180185190 ATTGCACTGATCATGAACTCCTCTGGATCTACTGGTCTGCCTAAAGGT624 IleAlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGly 195200205 GTCGCTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGA672 ValAlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArg 210215220 GATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGT720 AspProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSer 225230235240 GTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATAT768 ValValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyr 245250255 TTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAG816 LeuIleCysGlyPheArgValValLeuMetTyrArgPheGluGluGlu 260265270 CTGTTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCTGCTG864 LeuPheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeu 275280285 GTGCCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAA912 ValProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLys 290295300 TACGATTTATCTAATTTACACGAAATTGCTTCTGGTGGCGCTCCCCTC960 TyrAspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeu 305310315320 TCTAAGGAAGTCGGGGAAGCGGTTGCCAAGAGGTTCCATCTGCCAGGT1008 SerLysGluValGlyGluAlaValAlaLysArgPheHisLeuProGly 325330335 ATCAGGCAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATT1056 IleArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIle 340345350 ACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCA1104 ThrProGluGlyAspAspLysProGlyAlaValGlyLysValValPro 355360365 TTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGC1152 PhePheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGly 370375380 GTTAATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCC1200 ValAsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSer 385390395400 GGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGAT1248 GlyTyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAsp 405410415 GGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACAC1296 GlyTrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHis 420425430 TTCTTCATCGTTGACCGCCTGAAGTCTCTGATTAAGTACAAAGGCTAT1344 PhePheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyr 435440445 CAGGTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCAACACCCCAAC1392 GlnValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsn 450455460 ATCTTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAA1440 IlePheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGlu 465470475480 CTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAA1488 LeuProAlaAlaValValValLeuGluHisGlyLysThrMetThrGlu 485490495 AAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAG1536 LysGluIleValAspTyrValAlaSerGlnValThrThrAlaLysLys 500505510 TTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACC1584 LeuArgGlyGlyValValPheValAspGluValProLysGlyLeuThr 515520525 GGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAG1632 GlyLysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLys 530535540 AAGGGCGGAAAGATCGCCGTG1653 LysGlyGlyLysIleAla 545550 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 550 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: MetValThrAspAlaLysAsnIleLysLysGlyProAlaProPheTyr 151015 ProLeuGluAspGlyThrAlaGlyGluGlnLeuHisLysAlaMetLys 202530 ArgTyrAlaLeuValProGlyThrIleAlaPheThrAspAlaHisIle 354045 GluValAspIleThrTyrAlaGluTyrPheGluMetSerValArgLeu 505560 AlaGluAlaMetLysArgTyrGlyLeuAsnThrAsnHisArgIleVal 65707580 ValCysSerGluAsnSerLeuGlnPhePheMetProValLeuGlyAla 859095 LeuPheIleGlyValAlaValAlaProAlaAsnAspIleTyrAsnGlu 100105110 ArgGluLeuLeuAsnSerMetGlyIleSerGlnProThrValValPhe 115120125 ValSerLysLysGlyLeuGlnLysIleLeuAsnValGlnLysLysLeu 130135140 ProIleIleGlnLysIleIleIleMetAspSerLysThrAspTyrGln 145150155160 GlyPheGlnSerMetTyrThrPheValThrSerHisLeuProProGly 165170175 PheAsnGluTyrAspPheValProGluSerPheAspArgAspLysThr 180185190 IleAlaLeuIleMetAsnSerSerGlySerThrGlyLeuProLysGly 195200205 ValAlaLeuProHisArgThrAlaCysValArgPheSerHisAlaArg 210215220 AspProIlePheGlyAsnGlnIleIleProAspThrAlaIleLeuSer 225230235240 ValValProPheHisHisGlyPheGlyMetPheThrThrLeuGlyTyr 245250255 LeuIleCysGlyPheArgValValLeuMetTyrArgPheGluGluGlu 260265270 LeuPheLeuArgSerLeuGlnAspTyrLysIleGlnSerAlaLeuLeu 275280285 ValProThrLeuPheSerPhePheAlaLysSerThrLeuIleAspLys 290295300 TyrAspLeuSerAsnLeuHisGluIleAlaSerGlyGlyAlaProLeu 305310315320 SerLysGluValGlyGluAlaValAlaLysArgPheHisLeuProGly 325330335 IleArgGlnGlyTyrGlyLeuThrGluThrThrSerAlaIleLeuIle 340345350 ThrProGluGlyAspAspLysProGlyAlaValGlyLysValValPro 355360365 PhePheGluAlaLysValValAspLeuAspThrGlyLysThrLeuGly 370375380 ValAsnGlnArgGlyGluLeuCysValArgGlyProMetIleMetSer 385390395400 GlyTyrValAsnAsnProGluAlaThrAsnAlaLeuIleAspLysAsp 405410415 GlyTrpLeuHisSerGlyAspIleAlaTyrTrpAspGluAspGluHis 420425430 PhePheIleValAspArgLeuLysSerLeuIleLysTyrLysGlyTyr 435440445 GlnValAlaProAlaGluLeuGluSerIleLeuLeuGlnHisProAsn 450455460 IlePheAspAlaGlyValAlaGlyLeuProAspAspAspAlaGlyGlu 465470475480 LeuProAlaAlaValValValLeuGluHisGlyLysThrMetThrGlu 485490495 LysGluIleValAspTyrValAlaSerGlnValThrThrAlaLysLys 500505510 LeuArgGlyGlyValValPheValAspGluValProLysGlyLeuThr 515520525 GlyLysLeuAspAlaArgLysIleArgGluIleLeuIleLysAlaLys 530535540 LysGlyGlyLysIleAla 545550 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: TTCTATCCGCTGGAAGATGGAA22 PheTyrProLeuGluAspGly 15 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: PheTyrProLeuGluAspGly 15 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 12 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..12 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: GTGGTGTTCGTT12 ValValPheVal (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: ValValPheVal 1 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..8 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: TGCCGTAG8 CysArg 1 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: CysArg 1 (2) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..25 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: AAGAGGCGAACTGTGTGTGAGAGGT25 LysArgArgThrValCysGluArg 15 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: LysArgArgThrValCysGluArg 15 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..10 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: CAGGTGTCGC10 GlnValSer 1 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: GlnValSer 1 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 9 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..9 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: GACATCACT9 AspIleThr 1 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: AspIleThr 1 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 9 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (v) FRAGMENT TYPE: C-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Photinus pyralis (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..9 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: GGCATTTCG9 GlyIleSer 1 (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: GlyIleSer 1 __________________________________________________________________________

## Claims

1. A luciferase gene encoding a modified form of the luciferase of Photinus pyralis, wherein: NO: 1 is eliminated by changing the DNA sequence beginning at nucleotide 40 of SEQ. ID. NO: 1 to the DNA sequence of SEQ. ID. NO: 7, namely, TTC TAT CCG CTG GAA GAT GGA A; SEQ. ID. NO: 1 is altered by changing the DNA sequence beginning at nucleotide 373 of SEQ. ID. NO: 1 to SEQ. ID. NO: 9, namely, GTG GTG TTC GTT, wherein such change in DNA sequence is conducted without affecting the amino acid sequence; SEQ. ID. NO: 1 is altered by changing the DNA sequence of SEQ. ID. NO: 1 at nucleotide 646 to SEQ. ID. NO: 11, namely, TGC CGT AG, wherein such change in DNA sequence is conducted without affecting the amino acid sequence; SEQ. ID. NO: 1 is altered by changing the DNA sequence of SEQ. ID. NO: 1 at nucleotide 1158 to SEQ. ID. NO: 13, namely, AAG AGG CGA ACT GTG TGT GAG AGG T, wherein such change is conducted without affecting the amino acid sequence; 1400 of SEQ. ID. NO: 1 are altered by changing the DNA sequence of SEQ. ID. NO: 1 at nucleotide 40 to SEQ. ID. NO: 15, namely, CAG GTG TCG C, wherein such change is conducted without affecting the amino acid sequence; eliminated by replacing asparagine with aspartate; eliminated by replacing the tripeptide codon sequence beginning at nucleotide 148 of SEQ. ID. NO: 1 with SEQ. ID. NO: 17, namely, GAC ATC ACT, such that the amino acid sequence asparagine-isoleucine-threonine is replaced with aspartate-isoleucine-threonine; eliminated by replacing asparagine with glycine; and eliminated by replacing the tripeptide codon sequence beginning at nucleotide 355 of SEQ. ID. NO: 1 with SEQ. ID. NO. 19, namely, GGC ATT TCG, such that the amino acid sequence asparagine-isoleucine-serine is replaced with glycine-isoleucine-serine.

2. A recombinant DNA comprising the gene of claim 1.

3. A host cell containing the recombinant DNA of claim 2.

4. A modified luciferase gene having the nucleotide sequence illustrated in SEQ. ID. NO. 3.

5. A modified luciferase gene which encodes the amino acid sequence illustrated in SEQ. ID. NO. 4.

6. A luciferase gene encoding luciferase of Photinus pyralis comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase.

7. The luciferase gene according to claim 6, wherein the DNA sequence at nucleotides 48 through 54 of SEQ ID NO: 1 is changed from T CTA GAG to G CTG GAA.

8. The luciferase gene according to claim 6, further comprising a DNA which encodes a functional luciferase gene product having a C-terminal amino acid sequence of glycine-lysine-threonine.

9. The luciferase gene according to claim 6, further comprising a DNA which encodes a functional luciferase gene product having a C-terminal amino acid sequence of isoleucine-alanine-valine.

10. A method of using a luciferase gene to eliminate peroxisomal influence on expression of a luciferase gene product in a heterologous eucaryotic host comprising: a cytoplasmic form of a luciferase gene product into the heterologous eucaryotic host, whereby peroxisomal influence on the expression of the luciferase gene product is eliminated, the mutated luciferase gene selected from the group consisting of a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase, a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of glycine-lysine-threonine, and a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of isoleucine-alanine-valine.

11. The method according to claim 10, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase is stably or transiently incorporated into the heterologous eucaryotic host.

12. The method according to claim 10, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of glycine-lysine-threonine is stably or transiently incorporated into the heterologous eucaryotic host.

13. The method according to claim 10, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of isoleucine-alanine-valine is stably or transiently incorporated into the heterologous eucaryotic host.

14. A method of using a luciferase gene to increase total activity of a luciferase gene product in a heterologous eucaryotic host comprising: a cytoplasmic form of a luciferase gene product into the heterologous eucaryotic host, whereby total activity of the luciferase gene product is increased, the mutated luciferase gene selected from the group consisting of a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase, a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of glycine-lysine-threonine, and a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of isoleucine-alanine-valine.

15. The method according to claim 14, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase is stably or transiently incorporated into the heterologous eucaryotic host.

16. The method according to claim 14, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of glycine-lysine-threonine is stably or transiently incorporated into the heterologous eucaryotic host.

17. The method according to claim 14, wherein the luciferase gene comprising a DNA encoding functional luciferase wherein an Xba I restriction site beginning at nucleotide 48 of SEQ ID NO: 1 and a 22-base pair imperfect palindrome centered at the Xba I restriction site of SEQ ID NO: 1 are disrupted without affecting the amino acid sequence of the encoded luciferase and wherein the DNA encodes a gene product having a C-terminal amino acid sequence of isoleucine-alanine-valine is stably or transiently incorporated into the heterologous eucaryotic host.