# Patent

## Title
Use of Cry1Ab in combination with Cry1Be for management of resistant insects

## Abstract

The subject invention includes methods and plants for controlling European corn borer and/or fall armyworm insects, said plants comprising a Cry1Ab insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a national phase application, filed pursuant to 35 U.S.C. §371, of PCT application No. PCT/US2010/060830, filed on Dec. 16, 2010, which claims the benefit of U.S. provisional application No. 61/284,290, filed on Dec. 16, 2009. The prior applications are incorporated herein by reference in their entirety.


BACKGROUND OF THE INVENTION
Humans grow corn for food and energy applications. Humans also grow many other crops, including soybeans and cotton. Insects eat and damage plants and thereby undermine these human efforts. Billions of dollars are spent each year to control insect pests and additional billions are lost to the damage they inflict. Synthetic organic chemical insecticides have been the primary tools used to control insect pests but biological insecticides, such as the insecticidal proteins derived from Bacillus thuringiensis (Bt), have played an important role in some areas. The ability to produce insect-resistant plants through transformation with Bt insecticidal protein genes has revolutionized modern agriculture and heightened the importance and value of insecticidal proteins and their genes.
Several Bt proteins have been used to create the insect-resistant transgenic plants that have been successfully registered and commercialized to date. These include Cry1Ab, Cry1Ac, Cry1F and Cry3Bb in corn, Cry1Ac and Cry2Ab in cotton, and Cry3A in potato.
The commercial products expressing these proteins express a single protein except in cases where the combined insecticidal spectrum of 2 proteins is desired (e.g., Cry1Ab and Cry3Bb in corn combined to provide resistance to lepidopteran pests and rootworm, respectively) or where the independent action of the proteins makes them useful as a tool for delaying the development of resistance in susceptible insect populations (e.g., Cry1Ac and Cry2Ab in cotton combined to provide resistance management for tobacco budworm). See also U.S. Patent Application Publication No. 2009/0313717, which relates to a Cry2 protein plus a Vip3Aa, Cry1F, or Cry1A for control of Helicoverpa zea or armigerain. WO 2009/132850 relates to Cry1F or Cry1A and Vip3Aa for controlling Spodoptera frugiperda. U.S. Patent Application Publication No. 2008/0311096 relates in part to Cry1Ab for controlling Cry1F-resistant European corn borer (ECB; Ostrinia nubilalis (Hübner)).
That is, some of the qualities of insect-resistant transgenic plants that have led to rapid and widespread adoption of this technology also give rise to the concern that pest populations will develop resistance to the insecticidal proteins produced by these plants. Several strategies have been suggested for preserving the utility of Bt-based insect resistance traits which include deploying proteins at a high dose in combination with a refuge, and alternation with, or co-deployment of, different toxins (McGaughey et al. (1998), “B.t. Resistance Management,” Nature Biotechnol. 16:144-146).
The proteins selected for use in an insect resistant management (IRM) stack need to exert their insecticidal effect independently so that resistance developed to one protein does not confer resistance to the second protein (i.e., there is not cross resistance to the proteins). If, for example, a pest population selected for resistance to “Protein A” is sensitive to “Protein B”, one would conclude that there is not cross resistance and that a combination of Protein A and Protein B would be effective in delaying resistance to Protein A alone.
In the absence of resistant insect populations, assessments can be made based on other characteristics presumed to be related to mechanism of action and cross-resistance potential. The utility of receptor-mediated binding in identifying insecticidal proteins likely to not exhibit cross resistance has been suggested (van Mellaert et al. 1999). The key predictor of lack of cross resistance inherent in this approach is that the insecticidal proteins do not compete for receptors in a sensitive insect species.
In the event that two Bt toxins compete for the same receptor in an insect, then if that receptor mutates in that insect so that one of the toxins no longer binds to that receptor and thus is no longer insecticidal against the insect, it might be the case that the insect will also be resistant to the second toxin (which competitively bound to the same receptor). That is, the insect is cross-resistant to both Bt toxins. However, if two toxins bind to two different receptors, this could be an indication that the insect would not be simultaneously resistant to those two toxins.
For example, Cry1Fa protein is useful in controlling many lepidopteran pests species including ECB and the fall armyworm (FAW; Spodoptera frugiperda), and is active against the sugarcane borer (SCB; Diatraea saccharalis). The Cry1Fa protein, as produced in transgenic corn plants containing event TC1507, is responsible for an industry-leading insect resistance trait for FAW control. Cry1Fa is further deployed in the Herculex®, SmartStaxTM, and WideStrikeTM products.
The ability to conduct (competitive or homologous) receptor binding studies using Cry1Fa protein has been limited because a common technique available for labeling proteins for detection in receptor binding assays tends to inactivate the insecticidal activity of the Cry1Fa protein.
Additional Cry toxins are listed at the website of the official B.t. nomenclature committee (Crickmore et al.; lifesci.sussex.ac.uldhome/Neil_Crickmore/Bt/). There are currently nearly 60 main groups of “Cry” toxins (Cry1-Cry59), with additional Cyt toxins and VIP toxins and the like. Many of each numeric group have capital-letter subgroups, and the capital letter subgroups have lower-cased letter sub-subgroups. (Cry1 has A-L, and Cry1A has a-i, for example).
BRIEF SUMMARY OF THE INVENTION
The subject invention relates in part to the surprising discovery that Cry1Ab and Cry1Be do not compete for binding to sites in European corn borer (ECB; Ostrinia nubilalis (Hübner)) or fall armyworm (FAW; Spodoptera frugiperda) gut cell membrane preparations. As one skilled in the art will recognize with the benefit of this disclosure, plants that produce both of these proteins (including insecticidal portions of the full-length proteins) can delay or prevent the development of resistance to any of these insecticidal proteins alone. Corn and soybean are some preferred plants. ECB is the preferred target insect for the subject pair of toxins.
Thus, the subject invention relates in part to the use of a Cry1Ab protein in combination with a Cry1Be protein. Plants (and acreage planted with such plants) that produce both of these proteins are included within the scope of the subject invention.
The subject invention also relates in part to triple stacks or “pyramids” of three (or more) toxins, with Cry1Ab and Cry1Be being the base pair. In some preferred pyramid embodiments, the combination of the selected toxins provides three sites of action against ECB. Some preferred “three sites of action” pyramid combinations include the subject base pair of proteins plus Cry2A, Cry1I, and DIG-3 as the third protein for targeting ECB. These particular triple stacks would, according to the subject invention, advantageously and surprisingly provide three sites of action against ECB. This can help to reduce or eliminate the requirement for refuge acreage.
Although the subject invention is disclosed herein as a base pair of toxins, Cry1Ab and Cry1Be, which, either alone or in a “pyramid” of three or more toxins, provides for insect-resistance against ECB in corn, it should be understood that the combinations of Cry1Ab and Cry1Be, described herein, can be also used with additional proteins for targeting FAW in both soybean or corn.
Additional toxins/genes can also be added according to the subject invention. For example, if Cry1Fa is stacked with the subject pair of proteins (Cry1Fa and Cry1Be are both active against both FAW and ECB), adding one additional protein to this triple stack wherein the fourth added protein targets ECB, would provide three sites of action against FAW, and three sites of action against ECB. This added protein (the fourth protein for targeting FAW) could be selected from the group consisting of Cry1Fa, Vip3Ab, or Cry1E. This would result in a four-protein stack having three sites of action against two insects (ECB and FAW).
BRIEF DESCRIPTION OF THE TABLES
Table 1: provides examples of Amino Acids within the Four Classes of Amino Acids.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1: is a graph illustrating the percent binding of labeled Cry1Ab versus Cry1Be for ECB BBMVs.
FIG. 2: is a graph illustrating the percent binding of labeled Cry1Ab versus Cry1Be with FAW BBMVs.
FIG. 3: is a graph illustrating the percent binding of labeled Cry1Be versus Cry1Ab with FAW BBMVs.



DETAILED DESCRIPTION OF THE INVENTION
The subject invention relates in part to the surprising discovery that Cry1Ab and Cry1Be do not compete with each other for binding sites in the gut of the European corn borer (ECB; Ostrinia nubilalis (Hübner)) or the fall armyworms (FAW; Spodoptera frugiperda). Thus, a Cry1Ab protein can be used in combination with a Cry1Be protein in transgenic corn (and other plants; e.g., cotton and soybeans, for example) to delay or prevent ECB from developing resistance to either of these proteins alone. The subject pair of proteins can be effective at protecting plants (such as maize plants) from damage by Cry-resistant ECB. That is, one use of the subject invention is to protect corn and other economically important plant species from damage and yield loss caused by ECB populations that could develop resistance to Cry1Ab or Cry1Be.
The subject invention thus teaches an insect resistant management (IRM) stack comprising Cry1Ab and Cry1Be to prevent or mitigate the development of resistance by ECB to either or both of these proteins.
Further, although the subject invention, disclosed herein, teaches an IRM stack comprising Cry1Ab and Cry1Be for preventing resistance by ECB to either or both of these proteins, it is within the scope of the invention disclosed herein that one or both of Cry1Ab and Cry1Be may be adapted, either alone or in combination, to prevent resistance by FAW to either or both of these proteins.
The present invention provides compositions for controlling lepidopteran pests comprising cells that produce a Cry1Ab core toxin-containing protein and a Cry1Be core toxin-containing protein.
The invention further comprises a host transformed to produce both a Cry1Ab insecticidal protein and a Cry1Be insecticidal protein, wherein said host is a microorganism or a plant cell. The subject polynucleotide(s) are preferably in a genetic construct under control of a non-Bacillus-thuringiensis promoter(s). The subject polynucleotides can comprise codon usage for enhanced expression in a plant.
It is additionally intended that the invention provides a method of controlling lepidopteran pests comprising contacting said pests or the environment of said pests with an effective amount of a composition that contains a Cry1Ab insecticidal protein and further contains a Cry1Be insecticidal protein.
An embodiment of the invention comprises a maize plant comprising a plant-expressible gene encoding a Cry1Be core toxin-containing protein and a plant-expressible gene encoding a Cry1Ab core toxin-containing protein, and seed of such a plant.
A further embodiment of the invention comprises a maize plant wherein a plant-expressible gene encoding a Cry1Be insecticidal protein and a plant-expressible gene encoding a Cry1Ab insecticidal protein have been introgressed into said maize plant, and seed of such a plant.
As described in the Examples, competitive receptor binding studies using radiolabeled Cry1Ab and Cry1Be proteins show that the Cry1Be protein does not compete for binding in ECB or FAW tissues to which Cry1Ab binds. These results also indicate that the combination of Cry1Ab and Cry1Be proteins can be an effective means to mitigate the development of resistance in ECB and FAW populations to either of these proteins. Thus, based in part on the data described herein, it is thought that co-production (stacking) of the Cry1Be and Cry1Ab proteins can be used to produce a high dose IRM stack for ECB.
Other proteins can be added to this pair. For example, the subject invention also relates in part to triple stacks or “pyramids” of three (or more) toxins, with Cry1Ab and Cry1Be being the base pair. In some preferred pyramid embodiments, the selected toxins have three separate sites of action against FAW. Some preferred “three sites of action” pyramid combinations include the subject base pair of proteins plus Cry1Fa, Vip3Ab, Cry1C, Cry1 D, or Cry1E as the third protein for targeting FAW. These particular triple stacks would, according to the subject invention, advantageously and surprisingly provide three sites of action against FAW. This can help to reduce or eliminate the requirement for refuge acreage. By “separate sites of action,” it is meant any of the given proteins do not cause cross-resistance with each other.
Additional toxins/genes can also be added according to the subject invention. For example, if Cry1Fa is stacked with the subject pair of proteins (both Cry1Fa and Cry1Be are both active against both FAW and European cornborer (ECB)), adding one additional protein to this triple stack wherein the fourth added protein targets ECB, would provide three sites of action against FAW, and three sites of action against ECB. This added protein (the fourth protein) could be selected from the group consisting of Cry2A, Cry1 I, and DIG-3 (see U.S. Patent Application Ser. No. 61/284,278 (filed Dec. 16, 2009) and US 2010 00269223). This would result in a four-protein stack having three sites of action against two insects (ECB and FAW).
Thus, one deployment option is to use the subject pair of proteins in combination with a third toxin/gene, and to use this triple stack to mitigate the development of resistance in ECB and/or FAW to any of these toxins. Accordingly, the subject invention also relates in part to triple stacks or “pyramids” of three (or more) toxins. In some preferred pyramid embodiments, the selected toxins have three separate sites of action against ECB and/or FAW.
Included among deployment options of the subject invention would be to use two, three, or more proteins of the subject proteins in crop-growing regions where ECB and/or FAW can develop resistant populations.
For guidance regarding the use of Cry1Fa and Cry1Be (for controlling ECB and/or FAW), see U.S. Patent Application Ser. No. 61/284,290 (filed Dec. 16, 2009). With Cry1Fa being active against ECB (and FAW), Cry1Ab plus Cry1Be plus Cry1 Fa would, according to the subject invention, advantageously and surprisingly provide three sites of action against ECB. This can help to reduce or eliminate the requirement for refuge acreage.
Cry1 Fa is deployed in the Herculex®, SmartStaxTM, and WidesStrikeTM products. The subject pair of genes (Cry1Ab and Cry1Be) could be combined into, for example, a Cry1Fa product such as Herculex®, SmartStaxTM, and WideStrikeTM. Accordingly, the subject pair of proteins could be significant in reducing the selection pressure on these and other proteins. The subject pair of proteins could thus be used as in the three gene combinations for corn and other plants (cotton and soybeans, for example).
As discussed above, additional toxins/genes can also be added according to the subject invention. For targeting ECB, Cry2A, Cry11, and/or DIG-3 can be used. See U.S. Patent Application Ser. No. 61/284,278 (filed Dec. 16, 2009) and US 2010 00269223. For the combination of Cry1F and Cry1Ab (for controlling ECB), see U.S. Patent Application Publication No. 2008/0311096.
Plants (and acreage planted with such plants) that produce any of the subject combinations of proteins are included within the scope of the subject invention. Additional toxins/genes can also be added, but the particular stacks discussed above advantageously and surprisingly provide multiple sites of action against ECB and/or FAW. This can help to reduce or eliminate the requirement for refuge acreage. A field thus planted of over ten acres is thus included within the subject invention.
GENBANK can also be used to obtain the sequences for any of the genes and proteins discussed herein. See Appendix A, below. Patents can also be used. For example, U.S. Pat. No. 5,188,960 and U.S. Pat. No. 5,827,514 describe Cry1Fa core toxin containing proteins suitable for use in carrying out the present invention. U.S. Pat. No. 6,218,188 describes plant-optimized DNA sequences encoding Cry1Fa core toxin-containing proteins that are suitable for use in the present invention.
Combinations of proteins described herein can be used to control lepidopteran pests. Adult lepidopterans, for example, butterflies and moths, primarily feed on flower nectar and are a significant effector of pollination. Nearly all lepidopteran larvae, i.e., caterpillars, feed on plants, and many are serious pests. Caterpillars feed on or inside foliage or on the roots or stem of a plant, depriving the plant of nutrients and often destroying the plant's physical support structure. Additionally, caterpillars feed on fruit, fabrics, and stored grains and flours, ruining these products for sale or severely diminishing their value. As used herein, reference to lepidopteran pests refers to various life stages of the pest, including larval stages.
Some chimeric toxins of the subject invention comprise a full N-terminal core toxin portion of a Bt toxin and, at some point past the end of the core toxin portion, the protein has a transition to a heterologous protoxin sequence. The N-terminal, insecticidally active, toxin portion of a Bt toxin is referred to as the “core” toxin. The transition from the core toxin segment to the heterologous protoxin segment can occur at approximately the toxin/protoxin junction or, in the alternative, a portion of the native protoxin (extending past the core toxin portion) can be retained, with the transition to the heterologous protoxin portion occurring downstream.
As an example, one chimeric toxin of the subject invention, is a full core toxin portion of Cry1Ab (approximately amino acids 1 to 601) and/or a heterologous protoxin (approximately amino acids 602 to the C-terminus). In one preferred embodiment, the portion of a chimeric toxin comprising the protoxin is derived from a Cry1Ab protein toxin. In a preferred embodiment, the portion of a chimeric toxin comprising the protoxin is derived from a Cry1Ab protein toxin.
A person skilled in this art will appreciate that Bt toxins, even within a certain class such as Cry1Be, will vary to some extent in length and the precise location of the transition from core toxin portion to protoxin portion. Typically, the Cry1Be toxins are about 1150 to about 1200 amino acids in length. The transition from core toxin portion to protoxin portion will typically occur at between about 50% to about 60% of the full length toxin. The chimeric toxin of the subject invention will include the full expanse of this N-terminal core toxin portion. Thus, the chimeric toxin will comprise at least about 50% of the full length of the Cry1Be protein. This will typically be at least about 590 amino acids. With regard to the protoxin portion, the full expanse of the Cry1 Ab protoxin portion extends from the end of the core toxin portion to the C-terminus of the molecule.
Genes and Toxins.
The genes and toxins useful according to the subject invention include not only the full length sequences disclosed but also fragments of these sequences, variants, mutants, and fusion proteins which retain the characteristic pesticidal activity of the toxins specifically exemplified herein. As used herein, the terms “variants” or “variations” of genes refer to nucleotide sequences which encode the same toxins or which encode equivalent toxins having pesticidal activity. As used herein, the term “equivalent toxins” refers to toxins having the same or essentially the same biological activity against the target pests as the claimed toxins.
As used herein, the boundaries represent approximately 95% (Cry1Ab's and Cry1Be's), 78% (Cry1A's and Cry1B's), and 45% (Cry1's) sequence identity, per “Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins,” N. Crickmore, D. R. Zeigler, J. Feitelson, E. Schnepf, J. Van Rie, D. Lereclus, J. Baum, and D. H. Dean. Microbiology and Molecular Biology Reviews (1998) Vol 62: 807-813. These cut offs can also be applied to the core toxins only.
It should be apparent to a person skilled in this art that genes encoding active toxins can be identified and obtained through several means. The specific genes or gene portions exemplified herein may be obtained from the isolates deposited at a culture depository. These genes, or portions or variants thereof, may also be constructed synthetically, for example, by use of a gene synthesizer. Variations of genes may be readily constructed using standard techniques for making point mutations. Also, fragments of these genes can be made using commercially available exonucleases or endonucleases according to standard procedures. For example, enzymes such as Bal31 or site-directed mutagenesis can be used to systematically cut off nucleotides from the ends of these genes. Genes that encode active fragments may also be obtained using a variety of restriction enzymes. Proteases may be used to directly obtain active fragments of these protein toxins.
Fragments and equivalents which retain the pesticidal activity of the exemplified toxins would be within the scope of the subject invention. Also, because of the redundancy of the genetic code, a variety of different DNA sequences can encode the amino, acid sequences disclosed herein. It is well within the skill of a person trained in the art to create these alternative DNA sequences encoding the same, or essentially the same, toxins. These variant DNA sequences are within the scope of the subject invention. As used herein, reference to “essentially the same” sequence refers to sequences which have amino acid substitutions, deletions, additions, or insertions which do not materially affect pesticidal activity. Fragments of genes encoding proteins that retain pesticidal activity are also included in this definition.
A further method for identifying the genes encoding the toxins and gene portions useful according to the subject invention is through the use of oligonucleotide probes. These probes are detectable nucleotide sequences. These sequences may be detectable by virtue of an appropriate label or may be made inherently fluorescent as described in International Application No. WO93/16094. As is well known in the art, if the probe molecule and nucleic acid sample hybridize by forming a strong bond between the two molecules, it can be reasonably assumed that the probe and sample have substantial homology. Preferably, hybridization is conducted under stringent conditions by techniques well-known in the art, as described, for example, in Keller, G. H., M. M. Manak (1987) DNA Probes, Stockton Press, New York, N.Y., pp. 169-170. Some examples of salt concentrations and temperature combinations are as follows (in order of increasing stringency): 2×SSPE or SSC at room temperature; 1×SSPE or SSC at 42° C.; 0.1×SSPE or SSC at 42° C.; 0.1×SSPE or SSC at 65° C. Detection of the probe provides a means for determining in a known manner whether hybridization has occurred. Such a probe analysis provides a rapid method for identifying toxin-encoding genes of the subject invention. The nucleotide segments which are used as probes according to the invention can be synthesized using a DNA synthesizer and standard procedures. These nucleotide sequences can also be used as PCR primers to amplify genes of the subject invention.
Variant Toxins.
Certain toxins of the subject invention have been specifically exemplified herein. Since these toxins are merely exemplary of the toxins of the subject invention, it should be readily apparent that the subject invention comprises variant or equivalent toxins (and nucleotide sequences coding for equivalent toxins) having the same or similar pesticidal activity of the exemplified toxin. Equivalent toxins will have amino acid homology with an exemplified toxin. This amino acid homology will typically be greater than 75%, preferably be greater than 90%, and most preferably be greater than 95%. The amino acid homology will be highest in critical regions of the toxin which account for biological activity or are involved in the determination of three-dimensional configuration which ultimately is responsible for the biological activity. In this regard, certain amino acid substitutions are acceptable and can be expected if these substitutions are in regions which are not critical to activity or are conservative amino acid substitutions which do not affect the three-dimensional configuration of the molecule. For example, amino acids may be placed in the following classes: non-polar, uncharged polar, basic, and acidic. Conservative substitutions whereby an amino acid of one class is replaced with another amino acid of the same type fall within the scope of the subject invention so long as the substitution does not materially alter the biological activity of the compound. Below is a listing of examples of amino acids belonging to each class.







TABLE 1







Examples of Amino Acids within the Four Classes of Amino Acids










Class of Amino Acid
Examples of Amino Acids







Nonpolar
Ala, Val, Leu, Ile, Pro, Met, Phe, Trp



Uncharged Polar
Gly, Ser, Thr, Cys, Tyr, Asn, Gln



Acidic
Asp, Glu



Basic
Lys, Arg, His










In some instances, non-conservative substitutions can also be made. The critical factor is that these substitutions must not significantly detract from the biological activity of the toxin.
Recombinant Hosts.
The genes encoding the toxins of the subject invention can be introduced into a wide variety of microbial or plant hosts. Expression of the toxin gene results, directly or indirectly, in the intracellular production and maintenance of the pesticide. Conjugal transfer and recombinant transfer can be used to create a Bt strain that expresses both toxins of the subject invention. Other host organisms may also be transformed with one or both of the toxin genes then used to accomplish the synergistic effect. With suitable microbial hosts, e.g., Pseudomonas, the microbes can be applied to the situs of the pest, where they will proliferate and be ingested. The result is control of the pest. Alternatively, the microbe hosting the toxin gene can be treated under conditions that prolong the activity of the toxin and stabilize the cell. The treated cell, which retains the toxic activity, then can be applied to the environment of the target pest.
Where the Bt toxin gene is introduced via a suitable vector into a microbial host, and said host is applied to the environment in a living state, it is essential that certain host microbes be used. Microorganism hosts are selected which are known to occupy the “phytosphere” (phylloplane, phyllosphere, rhizosphere, and/or rhizoplane) of one or more crops of interest. These microorganisms are selected so as to be capable of successfully competing in the particular environment (crop and other insect habitats) with the wild-type microorganisms, provide for stable maintenance and expression of the gene expressing the polypeptide pesticide, and, desirably, provide for improved protection of the pesticide from environmental degradation and inactivation.
A large number of microorganisms are known to inhabit the phylloplane (the surface of the plant leaves) and/or the rhizosphere (the soil surrounding plant roots) of a wide variety of important crops. These microorganisms include bacteria, algae, and fungi. Of particular interest are microorganisms, such as bacteria, e.g., genera Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces, Rhizobium, Rhodopseudomonas, Methylophilius, Agrobactenum, Acetobacter, Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes; fungi, particularly yeast, e.g., genera Saccharomyces, Cryptococcus, Kluyveromyces, Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interest are such phytosphere bacterial species as Pseudomonas syringae, Pseudomonas fluorescens, Serratia marcescens, Acetobacter xylinum, Agrobactenium tumefaciens, Rhodopseudomonas spheroides, Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus, and Azotobacter vinlandii; and phytosphere yeast species such as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca, Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei, S. pretoriensis, S. cerevisiae, Sporobolomyces roseus, S. odorus, Kluyveromyces veronae, and Aureobasidium pollulans. Of particular interest are the pigmented microorganisms.
A wide variety of methods is available for introducing a Bt gene encoding a toxin into a microorganism host under conditions which allow for stable maintenance and expression of the gene. These methods are well known to those skilled in the art and are described, for example, in U.S. Pat. No. 5,135,867, which is incorporated herein by reference.
Treatment of Cells.
Bacillus thuringiensis or recombinant cells expressing the Bt toxins can be treated to prolong the toxin activity and stabilize the cell. The pesticide microcapsule that is formed comprises the Bt toxin or toxins within a cellular structure that has been stabilized and will protect the toxin when the microcapsule is applied to the environment of the target pest. Suitable host cells may include either prokaryotes or eukaryotes, normally being limited to those cells which do not produce substances toxic to higher organisms, such as mammals. However, organisms which produce substances toxic to higher organisms could be used, where the toxic substances are unstable or the level of application sufficiently low as to avoid any possibility of toxicity to a mammalian host. As hosts, of particular interest will be the prokaryotes and the lower eukaryotes, such as fungi.
The cell will usually be intact and be substantially in the proliferative form when treated, rather than in a spore form, although in some instances spores may be employed.
Treatment of the microbial cell, e.g., a microbe containing the Bt toxin gene or genes, can be by chemical or physical means, or by a combination of chemical and/or physical means, so long as the technique does not deleteriously affect the properties of the toxin, nor diminish the cellular capability of protecting the toxin. Examples of chemical reagents are halogenating agents, particularly halogens of atomic no. 17-80. More particularly, iodine can be used under mild conditions and for sufficient time to achieve the desired results. Other suitable techniques include treatment with aldehydes, such as glutaraldehyde; anti-infectives, such as zephiran chloride and cetylpyridinium chloride; alcohols, such as isopropyl and ethanol; various histologic fixatives, such as Lugol iodine, Bouin's fixative, various acids and Helly's fixative (See: Humason, Gretchen L., Animal Tissue Techniques, W. H. Freeman and Company, 1967); or a combination of physical (heat) and chemical agents that preserve and prolong the activity of the toxin produced in the cell when the cell is administered to the host environment. Examples of physical means are short wavelength radiation such as gamma-radiation and X-radiation, freezing, UV irradiation, lyophilization, and the like. Methods for treatment of microbial cells are disclosed in U.S. Pat. Nos. 4,695,455 and 4,695,462, which are incorporated herein by reference.
The cells generally will have enhanced structural stability which will enhance resistance to environmental conditions. Where the pesticide is in a proform, the method of cell treatment should be selected so as not to inhibit processing of the proform to the mature form of the pesticide by the target pest pathogen. For example, formaldehyde will crosslink proteins and could inhibit processing of the proform of a polypeptide pesticide. The method of treatment should retain at least a substantial portion of the bio-availability or bioactivity of the toxin.
Characteristics of particular interest in selecting a host cell for purposes of production include ease of introducing the Bt gene or genes into the host, availability of expression systems, efficiency of expression, stability of the pesticide in the host, and the presence of auxiliary genetic capabilities. Characteristics of interest for use as a pesticide microcapsule include protective qualities for the pesticide, such as thick cell walls, pigmentation, and intracellular packaging or formation of inclusion bodies; survival in aqueous environments; lack of mammalian toxicity; attractiveness to pests for ingestion; ease of killing and fixing without damage to the toxin; and the like. Other considerations include ease of formulation and handling, economics, storage stability, and the like.
Growth of Cells.
The cellular host containing the Bt insecticidal gene or genes may be grown in any convenient nutrient medium, where the DNA construct provides a selective advantage, providing for a selective medium so that substantially all or all of the cells retain the Bt gene. These cells may then be harvested in accordance with conventional ways. Alternatively, the cells can be treated prior to harvesting.
The Bt cells producing the toxins of the invention can be cultured using standard art media and fermentation techniques. Upon completion of the fermentation cycle the bacteria can be harvested by first separating the Bt spores and crystals from the fermentation broth by means well known in the art. The recovered Bt spores and crystals can be formulated into a wettable powder, liquid concentrate, granules or other formulations by the addition of surfactants, dispersants, inert carriers, and other components to facilitate handling and application for particular target pests. These formulations and application procedures are all well known in the art.
Formulations.
Formulated bait granules containing an attractant and spores, crystals, and toxins of the Bt isolates, or recombinant microbes comprising the genes obtainable from the Bt isolates disclosed herein, can be applied to the soil. Formulated product can also be applied as a seed-coating or root treatment or total plant treatment at later stages of the crop cycle. Plant and soil treatments of Bt cells may be employed as wettable powders, granules or dusts, by mixing with various inert materials, such as inorganic minerals (phyllosilicates, carbonates, sulfates, phosphates, and the like) or botanical materials (powdered corncobs, rice hulls, walnut shells, and the like). The formulations may include spreader-sticker adjuvants, stabilizing agents, other pesticidal additives, or surfactants. Liquid formulations may be aqueous-based or non-aqueous and employed as foams, gels, suspensions, emulsifiable concentrates, or the like. The ingredients may include rheological agents, surfactants, emulsifiers, dispersants, or polymers.
As would be appreciated by a person skilled in the art, the pesticidal concentration will vary widely depending upon the nature of the particular formulation, particularly whether it is a concentrate or to be used directly. The pesticide will be present in at least 1% by weight and may be 100% by weight. The dry formulations will have from about 1-95% by weight of the pesticide while the liquid formulations will generally be from about 1-60% by weight of the solids in the liquid phase. The formulations will generally have from about 102 to about 104 cells/mg. These formulations will be administered at about 50 mg (liquid or dry) to 1 kg or more per hectare.
The formulations can be applied to the environment of the lepidopteran pest, e.g., foliage or soil, by spraying, dusting, sprinkling, or the like.
Plant Transformation.
A preferred recombinant host for production of the insecticidal proteins of the subject invention is a transformed plant. Genes encoding Bt toxin proteins, as disclosed herein, can be inserted into plant cells using a variety of techniques which are well known in the art. For example, a large number of cloning vectors comprising a replication system in Escherichia coli and a marker that permits selection of the transformed cells are available for preparation for the insertion of foreign genes into higher plants. The vectors comprise, for example, pBR322, pUC series, M13 mp series, pACYC184, inter alia. Accordingly, the DNA fragment having the sequence encoding the Bt toxin protein can be inserted into the vector at a suitable restriction site. The resulting plasmid is used for transformation into E. coli. The E. coli cells are cultivated in a suitable nutrient medium, then harvested and lysed. The plasmid is recovered. Sequence analysis, restriction analysis, electrophoresis, and other biochemical-molecular biological methods are generally carried out as methods of analysis. After each manipulation, the DNA sequence used can be cleaved and joined to the next DNA sequence. Each plasmid sequence can be cloned in the same or other plasmids. Depending on the method of inserting desired genes into the plant, other DNA sequences may be necessary. If, for example, the Ti or Ri plasmid is used for the transformation of the plant cell, then at least the right border, but often the right and the left border of the Ti or Ri plasmid T-DNA, has to be joined as the flanking region of the genes to be inserted. The use of T-DNA for the transformation of plant cells has been intensively researched and sufficiently described in EP 120 516, Lee and Gelvin (2008), Hoekema (1985), Fraley et al., (1986), and An et al., (1985), and is well established in the art.
Once the inserted DNA has been integrated in the plant genome, it is relatively stable. The transformation vector normally contains a selectable marker that confers on the transformed plant cells resistance to a biocide or an antibiotic, such as Bialaphos, Kanamycin, G418, Bleomycin, or Hygromycin, inter alia. The individually employed marker should accordingly permit the selection of transformed cells rather than cells that do not contain the inserted DNA.
A large number of techniques are available for inserting DNA into a plant host cell. Those techniques include transformation with T-DNA using Agrobacterium tumefaciens or Agrobacterium rhizogenes as transformation agent, fusion, injection, biolistics (microparticle bombardment), or electroporation as well as other possible methods. If Agrobacteria are used for the transformation, the DNA to be inserted has to be cloned into special plasmids, namely either into an intermediate vector or into a binary vector. The intermediate vectors can be integrated into the Ti or Ri plasmid by homologous recombination owing to sequences that are homologous to sequences in the T-DNA. The Ti or Ri plasmid also comprises the vir region necessary for the transfer of the T-DNA. Intermediate vectors cannot replicate themselves in Agrobacteria. The intermediate vector can be transferred into Agrobacterium tumefaciens by means of a helper plasmid (conjugation). Binary vectors can replicate themselves both in E. coli and in Agrobacteria. They comprise a selection marker gene and a linker or polylinker which are framed by the Right and Left T-DNA border regions. They can be transformed directly into Agrobacteria (Holsters et al., 1978). The Agrobacterium used as host cell is to comprise a plasmid carrying a vir region. The vir region is necessary for the transfer of the T-DNA into the plant cell. Additional T-DNA may be contained. The bacterium so transformed is used for the transformation of plant cells. Plant explants can advantageously be cultivated with Agrobacterium tumefaciens or Agrobacterium rhizogenes for the transfer of the DNA into the plant cell. Whole plants can then be regenerated from the infected plant material (for example, pieces of leaf, segments of stalk, roots, but also protoplasts or suspension-cultivated cells) in a suitable medium, which may contain antibiotics or biocides for selection. The plants so obtained can then be tested for the presence of the inserted DNA. No special demands are made of the plasmids in the case of injection and electroporation. It is possible to use ordinary plasmids, such as, for example, pUC derivatives.
The transformed cells grow inside the plants in the usual manner. They can form germ cells and transmit the transformed trait(s) to progeny plants. Such plants can be grown in the normal manner and crossed with plants that have the same transformed hereditary factors or other hereditary factors. The resulting hybrid individuals have the corresponding phenotypic properties.
In a preferred embodiment of the subject invention, plants will be transformed with genes wherein the codon usage has been optimized for plants. See, for example, U.S. Pat. No. 5,380,831, which is hereby incorporated by reference. While some truncated toxins are exemplified herein, it is well-known in the Bt art that 130 kDa-type (full-length) toxins have an N-terminal half that is the core toxin, and a C-terminal half that is the protoxin “tail.” Thus, appropriate “tails” can be used with truncated/core toxins of the subject invention. See e.g. U.S. Pat. No. 6,218,188 and U.S. Pat. No. 6,673,990. In addition, methods for creating synthetic Bt genes for use in plants are known in the art (Stewart and Burgin, 2007). One non-limiting example of a preferred transformed plant is a fertile maize plant comprising a plant expressible gene encoding a Cry1Ab protein, and further comprising a second plant expressible gene encoding a Cry1Be protein.
Transfer (or introgression) of the Cry1Ab- and Cry1Be-determined trait(s) into inbred maize lines can be achieved by recurrent selection breeding, for example by backcrossing. In this case, a desired recurrent parent is first crossed to a donor inbred (the non-recurrent parent) that carries the appropriate gene(s) for the Cry1A- and Cry1Be-determined traits. The progeny of this cross is then mated back to the recurrent parent followed by selection in the resultant progeny for the desired trait(s) to be transferred from the non-recurrent parent. After three, preferably four, more preferably five or more generations of backcrosses with the recurrent parent with selection for the desired trait(s), the progeny will be heterozygous for loci controlling the trait(s) being transferred, but will be like the recurrent parent for most or almost all other genes (see, for example, Poehlman & Sleper (1995) Breeding Field Crops, 4th Ed., 172-175; Fehr (1987) Principles of Cultivar Development, Vol. 1: Theory and Technique, 360-376).
Insect Resistance Management (IRM) Strategies.
Roush et al., for example, outlines two-toxin strategies, also called “pyramiding” or “stacking,” for management of insecticidal transgenic crops. (The Royal Society. Phil. Trans. R. Soc. Lond. B. (1998) 353, 1777-1786).
On their website, the United States Environmental Protection Agency (epa.gov/oppbppd1/biopesticides/pips/bt_corn_refuge_2006.htm) publishes the following requirements for providing non-transgenic (i.e., non-B.t.) refuges (a section of non-Bt crops/corn) for use with transgenic crops producing a single Bt protein active against target pests.

    
    
        “The specific structured requirements for corn borer-protected Bt (Cry1Ab or Cry1F) corn products are as follows:
        Structured refuges: 20% non-Lepidopteran Bt corn refuge in Corn Belt;
        
            50% non-Lepidopteran Bt refuge in Cotton Belt
        
        
        Blocks
        
            internal (i.e., within the Bt field)
            External (i.e., separate fields within V2 mile (1⁄4 mile if possible) of the
            Bt field to maximize random mating)
        
        
        In-Field Strips
        
            Strips must be at least 4 rows wide (preferably 6 rows) to reduce the effects of larval movement”
        
        
    
    


In addition, the National Corn Growers Association, on their website:

    
    
        (ncga.com/insect-resistance-management-fact-sheet-bt-corn)
    
    


It also provides similar guidance regarding the refuge requirements. For example:

    
    
        “Requirements of the Corn Borer IRM:
        
            Plant at least 20% of your corn acres to refuge hybrids
            In cotton producing regions, refuge must be 50%
            Must be planted within 1⁄2 mile of the refuge hybrids
            Refuge can be planted as strips within the Bt field; the refuge strips must be at least 4 rows wide
            Refuge may be treated with conventional pesticides only if economic thresholds are reached for target insect
            Bt-based sprayable insecticides cannot be used on the refuge corn
            Appropriate refuge must be planted on every farm with Bt corn”
        
        
    
    


As stated by Roush et al. (on pages 1780 and 1784 right column, for example), stacking or pyramiding of two different proteins each effective against the target pests and with little or no cross-resistance can allow for use of a smaller refuge. Roush suggests that for a successful stack, a refuge size of less than 10% refuge, can provide comparable resistance management to about 50% refuge for a single (non-pyramided) trait. For currently available pyramided Bt corn products, the U.S. Environmental Protection Agency requires significantly less (generally 5%) structured refuge of non-Bt corn be planted than for single trait products (generally 20%).
There are various ways of providing the IRM effects of a refuge, including various geometric planting patterns in the fields (as mentioned above) and in-bag seed mixtures, as discussed further by Roush et al. (supra), and U.S. Pat. No. 6,551,962.
The above percentages, or similar refuge ratios, can be used for the subject double or triple stacks or pyramids. For triple stacks with three sites of action against a single target pest, a goal would be zero refuge (or less than 5% refuge, for example). This is particularly true for commercial acreage—of over 10 acres for example.
All patents, patent applications, provisional applications, and publications referred to or cited herein are incorporated by reference in their entirety to the extent they are not inconsistent with the explicit teachings of this specification.
Unless specifically indicated or implied, the terms “a”, “an”, and “the” signify “at least one” as used herein.
Following are examples that illustrate procedures for practicing the invention. These examples should not be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted. All temperatures are in degrees Celsius.
EXAMPLES
Example 1
125I Labeling of Cry Proteins
Iodination of Cry Toxins.
Purified truncated Cry toxins were was iodinated using Iodo-Beads or Iodo-gen (Pierce). Briefly, two Iodo-Beads were washed twice with 500 μl of phosphate buffered saline, PBS (20 mM sodium phosphate, 0.15 M NaCl, pH 7.5), and placed into a 1.5 ml centrifuge tube behind lead shielding. To this was added 100 μl of PBS. In a hood and through the use of proper radioactive handling techniques, 0.5 mCi Na125I (17.4 Ci/mg, Lot 0114, Amersham) was added to the PBS solution with the Iodo-Bead. The components were allowed to react for 5 minutes at room temperature, then 2-25 μg of highly pure truncated Cry protein was added to the solution and allowed to react for an additional 3-5 minutes. The reaction was terminated by removing the solution from the iodo-beads and applying it to a 0.5 ml desalting Zeba spin column (InVitrogen) equilibrated in PBS. The iodo-bead was washed twice with 10 μl of PBS each and the wash solution also applied to the desalting column. The radioactive solution was eluted through the desalting column by centrifugation at 1,000×g for 2 min. In the case of Cry1Da, the Iodo-gen method was used to conduct the radiolabeling procedure. Using this procedure, the cry toxin in 100 mM phosphate buffer (pH 8) was first cleaned of lipopolysaccharides (LPS) by passing it through a small 0.5 ml polymyxin column multiple times. To the iodo-gen tube (Pierce Chem. Co.) was added 20 μg of the LPS-free Cry1Da toxin, then 0.5 mCi of Na125I. The reaction mixture was shaken for 15 min at 25° C. The solution was removed from the tube, and 50 μl of 0.2M non-radiolabeled NaI added to quench the reaction. The protein was dialyzed vs PBS with 3 changes of buffer to remove any unbound 125I.
Radio-purity of the iodinated Cry proteins was determined by SDS-PAGE, phosphorimaging and gamma counting. Briefly, 2 μl of the radioactive protein was separated by SDS-PAGE. After separation, the gels were dried using a BioRad gel drying apparatus following the manufacturer's instructions. The dried gels were imaged by wrapping them in Mylar film (12 μm thick), and exposing them under a Molecular Dynamics storage phosphor screen (35 cm×43 cm), for 1 hour. The plates were developed using a Molecular Dynamics Storm 820 phosphorimager and the imaged analyzed using ImageQuantTM software. The radioactive band along with areas immediately above and below the band were cut from the gel using a razor blade and counted in a gamma counter. Radioactivity was only detected in the Cry protein band and in areas below the band. No radioactivity was detected above the band, indicating that all radioactive contaminants consisted of smaller protein components than the truncated Cry protein. These components most probably represent degradation products.
Example 2
BBMV Preparation Protocol
Preparation and Fractionation of Solubilized BBMV's.
Last instar Spodoptera frugiperda, Ostrinia nubilalis, or Heleothis zea larvae were fasted overnight and then dissected in the morning after chilling on ice for 15 minutes. The midgut tissue was removed from the body cavity, leaving behind the hindgut attached to the integument. The midgut was placed in 9× volume of ice cold homogenization buffer (300 mM mannitol, 5 mM EGTA, 17 mM tris. base, pH 7.5), supplemented with Protease Inhibitor Cocktail1 (Sigma P-2714) diluted as recommended by the supplier. The tissue was homogenized with 15 strokes of a glass tissue homogenizer. BBMV's were prepared by the MgCl2 precipitation method of Wolfersberger (1993). Briefly, an equal volume of a 24 mM MgCl2 solution in 300 mM mannitol was mixed with the midgut homogenate, stirred for 5 minutes and allowed to stand on ice for 15 min. The solution was centrifuged at 2,500×g for 15 min at 4° C. The supernatant was saved and the pellet suspended into the original volume of 0.5-× diluted homogenization buffer and centrifuged again. The two supernatants were combined, centrifuged at 27,000×g for 30 min at 4° C. to form the BBMV fraction. The pellet was suspended into 10 ml homogienization buffer and supplemented to protease inhibitiors and centrifuged again at 27,000×g of r30 min at 4° C. to wash the BBMV's. The resulting pellet was suspended into BBMV Storage Buffer (10 mM HEPES, 130 mM KCl, 10% glycerol, pH 7.4) to a concentration of about 3 mg/ml protein. Protein concentration was determined by using the Bradford method (1976) with bovine serum albumin (BSA) as the standard. Alkaline phosphatase determination was made prior to freezing the samples using the Sigma assay following manufacturer's instructions. The specific activity of this marker enzyme in the BBMV fraction typically increased 7-fold compared to that found in the midgut homogenate fraction. The BBMV's were aliquoted into 250 μl samples, flash frozen in liquid N2 and stored at −80° C. Final concentration of cocktail components (in μM) are AEBSF (500), EDTA (250 mM), Bestatin (32), E-64 (0.35). Leupeptin (0.25), and Aprotinin (0.075).
Example 3
Method to Measure Binding of 125I Cry Proteins to BBMV Proteins
Binding of 125I Cry Proteins to BBMV's.
To determine the optimal amount of BBMV protein to use in the binding assays, a saturation curve was generated. 125I radiolabeled Cry protein (0.5 nM) was incubated for 1 hr. at 28° C. with various amounts of BBMV protein, ranging from 0-500 μg/ml in binding buffer (8 mM NaHPO4, 2 mM KH2PO4, 150 mM NaCl, 0.1% bovine serum albumin, pH 7.4). Total volume was 0.5 ml. Bound 125I Cry protein was separated from unbound by sampling 150 μl of the reaction mixture in triplicate from a 1.5 ml centrifuge tube into a 500 μl centrifuge tube and centrifuging the samples at 14,000×g for 6 minutes at room temperature. The supernatant was gently removed, and the pellet gently washed three times with ice cold binding buffer. The bottom of the centrifuge containing the pellet was cut out and placed into a 13×75-mm glass culture tube. The samples were counted for 5 minutes each in the gamma counter. The counts contained in the sample were subtracted from background counts (reaction with out any protein) and was plotted versus BBMV protein concentration. The optimal amount of protein to use was determined to be 0.15 mg/ml of BBMV protein.
To determine the binding kinetics, a saturation curve was generated. Briefly, BBMV's (150 μg/ml) were incubated for 1 hr. at 28° C. with increasing concentrations of 125I Cry toxin, ranging from 0.01 to 10 nM. Total binding was determined by sampling 150 μl of each concentration in triplicate, centrifugation of the sample and counting as described above. Non-specific binding was determined in the same manner, with the addition of 1,000 nM of the homologous trypsinized non-radioactive Cry toxin added to the reaction mixture to saturate all non-specific receptor binding sites. Specific binding was calculated as the difference between total binding and non-specific binding.
Homologous and heterologous competition binding assays were conducted using 150 μg/ml BBMV protein and 0.5 nM of the 125I radiolabeled Cry protein. The concentration of the competitive non-radiolabeled Cry toxin added to the reaction mixture ranged from 0.045 to 1,000 nM and were added at the same time as the radioactive ligand, to assure true binding competition. Incubations were carried out for 1 hr. at 28° C. and the amount of 125I Cry protein bound to its receptor toxin measured as described above with non-specific binding subtracted. One hundred percent total binding was determined in the absence of any competitor ligand. Results were plotted on a semi-logarithmic plot as percent total specific binding versus concentration of competitive ligand added.
Example 4
Summary of Results
FIG. 1 shows percent specific binding of 125I Cry1Ab (0.5 nM) in BBMV's from ECB versus competition by unlabeled homologous Cry1Ab (♦) and heterologous Cry1Be (●). The displacement curve for homologous competition by Cry1Ab results in a sigmoidal shaped curve showing 50% displacement of the radioligand at about 0.5 nM of Cry1Ab. Cry1Be also displaces 125I Cry1Be from its binding site but requires approximately 40 nM concentration (80-fold higher than required by Cry1Ab), to displace 50% of the 125I Cry1Ab from its binding site.
FIG. 2 shows percent specific binding of 125I Cry1Ab (0.5 nM) in BBMV's from FAW versus competition by unlabeled homologous Cry1Ab (♦) and heterologous Cry1Be (●). The displacement curve for homologous competition by Cry1Ab results in a sigmoidal shaped curve showing 50% displacement of the radioligand at about 0.3 nM of Cry1Ab. Cry1Be displaces 125I Cry1Ab by 50% at a concentration of approximately 300 nM, or about 1,000-fold greater than required by Cry1Ab. Error bars represent the range of values obtained from duplicate determinations.
FIG. 3 shows percent specific binding of 125I Cry1Be (0.5 nM) in BBMV's from FAW versus competition by unlabeled homologous Cry1Be (♦) and heterologous Cry1Ab (●). The displacement curve for homologous competition by Cry1Be results in a sigmoidal shaped curve showing 50% displacement of the radioligand at about 2 nM of Cry1Be. Cry1Ab at a concentration of 1,000 nM (2,000-fold greater than 125I Cry1Be being displaced) results approximately 50% displacement. There is an approximate 500-fold lower affinity of Cry1Ab to compete for the binding of Cry1Be. Error bars represent the range of values obtained from triplicate determinations.
REFERENCE LIST


    Heckel, D. G., Gahan, L. J., Baxter, S. W., Zhao, J. Z., Shelton, A. M., Gould, F., and Tabashnik, B. E. (2007). The diversity of Bt resistance genes in species of Lepidoptera. J Invertebr Pathol 95, 192-197.
    Luo, K., Banks, D., and Adang, M. J. (1999). Toxicity, binding, and permeability analyses of four bacillus thuringiensis cry1 delta-endotoxins using brush border membrane vesicles of spodoptera exigua and spodoptera frugiperda. Appl. Environ. Microbiol. 65, 457-464.
    Palmer, M., Buchkremer, M, Valeva, A, and Bhakdi, S. Cysteine-specific radioiodination of proteins with fluorescein maleimide. Analytical Biochemistry 253, 175-179. 1997. Ref Type: Journal (Full)
    Sambrook, J. and Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory).
    Schlenz, M. L., Babcock, J. M., and Storer, N. P. Response of Cry1F-resistant and Susceptible European Corn Borer and Fall Armyworm Colonies to Cry1A. 105 and Cry12Ab2. DAI 0830, 2008. Indianapolis, Dow AgroSciences. Derbi Report.
    Sheets, J. J. and Storer, N. P. Analysis of Cry1Ac Binding to Proteins in Brush Border Membrane Vesicles of Corn Earworm Larvae (Heleothis zea). Interactions with Cry1F Proteins and Its Implication for Resistance in the Field. DAI-0417, 1-26. 2001. Indianapolis, Dow AgroSciences.
    Tabashnik, B. E., Liu, Y. B., Finson, N., Masson, L., and Heckel, D. G. (1997). One gene in diamondback moth confers resistance to four Bacillus thuringiensis toxins. Proc. Natl. Acad. Sci. U.S. A 94, 1640-1644.
    Tabashnik, B. E., Malvar, T., Liu, Y. B., Finson, N., Borthakur, D., Shin, B. S., Park, S. H., Masson, L., de Maagd, R. A., and Bosch, D. (1996). Cross-resistance of the diamondback moth indicates altered interactions with domain II of Bacillus thuringiensis toxins. Appl. Environ. Microbiol. 62, 2839-2844.
    Tabashnik, B. E., Roush, R. T., Earle, E. D., and Shelton, A. M. (2000). Resistance to Bt toxins. Science 287, 42.
    Wolfersberger, M. G. (1993). Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the gypsy moth (Lymantria dispar). Arch. Insect Biochem. Physiol 24, 139-147.
    Xu, X., Yu, L., and Wu, Y. (2005). Disruption of a cadherin gene associated with resistance to Cry1Ac {delta}-endotoxin of Bacillus thuringiensis in Helicoverpa armigera. Appl Environ Microbiol 71, 948-954.









APPENDIX A





List of delta-endotoxins - from Crickmore et al. website (cited in application)


Accession Number is to NCBI entry






















Name
Acc No.
Authors
Year
Source Strain
Comment







Cry1Aa1
AAA22353
Schnepf et al
1985
Bt kurstaki HD1



Cry1Aa2
AAA22552
Shibano et al
1985
Bt sotto



Cry1Aa3
BAA00257
Shimizu et al
1988
Bt aizawai IPL7



Cry1Aa4
CAA31886
Masson et al
1989
Bt entomocidus



Cry1Aa5
BAA04468
Udayasuriyan et al
1994
Bt Fu-2-7



Cry1Aa6
AAA86265
Masson et al
1994
Bt kurstaki NRD-







12



Cry1Aa7
AAD46139
Osman et al
1999
Bt C12



Cry1Aa8
I26149
Liu
1996

DNA sequence only



Cry1Aa9
BAA77213
Nagamatsu et al
1999
Bt dendrolimus







T84A1



Cry1Aa10
AAD55382
Hou and Chen
1999
Bt kurstaki HD-1-







02



Cry1Aa11
CAA70856
Tounsi et al
1999
Bt kurstaki



Cry1Aa12
AAP80146
Yao et al
2001
Bt Ly30



Cry1Aa13
AAM44305
Zhong et al
2002
Bt sotto



Cry1Aa14
AAP40639
Ren et al
2002
unpublished



Cry1Aa15
AAY66993
Sauka et al
2005
Bt INTA Mol-12



Cry1Ab1
AAA22330
Wabiko et al
1986
Bt berliner 1715



Cry1Ab2
AAA22613
Thorne et al
1986
Bt kurstaki



Cry1Ab3
AAA22561
Geiser et al
1986
Bt kurstaki HD1



Cry1Ab4
BAA00071
Kondo et al
1987
Bt kurstaki HD1



Cry1Ab5
CAA28405
Hofte et al
1986
Bt berliner 1715



Cry1Ab6
AAA22420
Hefford et al
1987
Bt kurstaki NRD-







12



Cry1Ab7
CAA31620
Haider & Ellar
1988
Bt aizawai IC1



Cry1Ab8
AAA22551
Oeda et al
1987
Bt aizawai IPL7



Cry1Ab9
CAA38701
Chak & Jen
1993
Bt aizawai HD133



Cry1Ab10
A29125
Fischhoff et al
1987
Bt kurstaki HD1



Cry1Ab11
I12419
Ely & Tippett
1995
Bt A20
DNA sequence only



Cry1Ab12
AAC64003
Silva-Werneck et al
1998
Bt kurstaki S93



Cry1Ab13
AAN76494
Tan et al
2002
Bt c005



Cry1Ab14
AAG16877
Meza-Basso &
2000
Native Chilean Bt





Theoduloz



Cry1Ab15
AAO13302
Li et al
2001
Bt B-Hm-16



Cry1Ab16
AAK55546
Yu et al
2002
Bt AC-11



Cry1Ab17
AAT46415
Huang et al
2004
Bt WB9



Cry1Ab18
AAQ88259
Stobdan et al
2004
Bt



Cry1Ab19
AAW31761
Zhong et al
2005
Bt X-2



Cry1Ab20
ABB72460
Liu et al
2006
BtC008



Cry1Ab21
ABS18384
Swiecicka et al
2007
Bt IS5056



Cry1Ab22
ABW87320
Wu and Feng
2008
BtS2491Ab



Cry1Ab-
AAK14336
Nagarathinam et al
2001
Bt kunthala RX24
uncertain sequence



like



Cry1Ab-
AAK14337
Nagarathinam et al
2001
Bt kunthala RX28
uncertain sequence



like



Cry1Ab-
AAK14338
Nagarathinam et al
2001
Bt kunthala RX27
uncertain sequence



like



Cry1Ab-
ABG88858
Lin et al
2006
Bt ly4a3
insufficient sequence



like



Cry1Ac1
AAA22331
Adang et al
1985
Bt kurstaki HD73



Cry1Ac2
AAA22338
Von Tersch et al
1991
Bt kenyae



Cry1Ac3
CAA38098
Dardenne et al
1990
Bt BTS89A



Cry1Ac4
AAA73077
Feitelson
1991
Bt kurstaki







PS85A1



Cry1Ac5
AAA22339
Feitelson
1992
Bt kurstaki







PS81GG



Cry1Ac6
AAA86266
Masson et al
1994
Bt kurstaki NRD-







12



Cry1Ac7
AAB46989
Herrera et al
1994
Bt kurstaki HD73



Cry1Ac8
AAC44841
Omolo et al
1997
Bt kurstaki HD73



Cry1Ac9
AAB49768
Gleave et al
1992
Bt DSIR732



Cry1Ac10
CAA05505
Sun
1997
Bt kurstaki YBT-







1520



Cry1Ac11
CAA10270
Makhdoom &
1998





Riazuddin



Cry1Ac12
I12418
Ely & Tippett
1995
Bt A20
DNA sequence only



Cry1Ac13
AAD38701
Qiao et al
1999
Bt kurstaki HD1



Cry1Ac14
AAQ06607
Yao et al
2002
Bt Ly30



Cry1Ac15
AAN07788
Tzeng et al
2001
Bt from Taiwan



Cry1Ac16
AAU87037
Zhao et al
2005
Bt H3



Cry1Ac17
AAX18704
Hire et al
2005
Bt kenyae HD549



Cry1Ac18
AAY88347
Kaur & Allam
2005
Bt SK-729



Cry1Ac19
ABD37053
Gao et al
2005
Bt C-33



Cry1Ac20
ABB89046
Tan et al
2005



Cry1Ac21
AAY66992
Sauka et al
2005
INTA Mol-12



Cry1Ac22
ABZ01836
Zhang & Fang
2008
Bt W015-1



Cry1Ac23
CAQ30431
Kashyap et al
2008
Bt



Cry1Ac24
ABL01535
Arango et al
2008
Bt 146-158-01



Cry1Ac25
FJ513324
Guan Peng et al
2008
Bt Tm37-6
No NCBI link July 09



Cry1Ac26
FJ617446
Guan Peng et al
2009
Bt Tm41-4
No NCBI link July 09



Cry1Ac27
FJ617447
Guan Peng et al
2009
Bt Tm44-1B
No NCBI link July 09



Cry1Ac28
ACM90319
Li et al
2009
Bt Q-12



Cry1Ad1
AAA22340
Feitelson
1993
Bt aizawai PS81I



Cry1Ad2
CAA01880
Anonymous
1995
Bt PS81RR1



Cry1Ae1
AAA22410
Lee & Aronson
1991
Bt alesti



Cry1Af1
AAB82749
Kang et al
1997
Bt NT0423



Cry1Ag1
AAD46137
Mustafa
1999



Cry1Ah1
AAQ14326
Tan et al
2000



Cry1Ah2
ABB76664
Qi et al
2005
Bt alesti



Cry1Ai1
AAO39719
Wang et al
2002



Cry1A-
AAK14339
Nagarathinam et al
2001
Bt kunthala nags3
uncertain sequence



like



Cry1Ba1
CAA29898
Brizzard & Whiteley
1988
Bt thuringiensis







HD2



Cry1Ba2
CAA65003
Soetaert
1996
Bt entomocidus







HD110



Cry1Ba3
AAK63251
Zhang et al
2001



Cry1Ba4
AAK51084
Nathan et al
2001
Bt entomocidus







HD9



Cry1Ba5
ABO20894
Song et al
2007
Bt sfw-12



Cry1Ba6
ABL60921
Martins et al
2006
Bt S601



Cry1Bb1
AAA22344
Donovan et al
1994
Bt EG5847



Cry1Bc1
CAA86568
Bishop et al
1994
Bt morrisoni



Cry1Bd1
AAD10292
Kuo et al
2000
Bt wuhanensis







HD525



Cry1Bd2
AAM93496
Isakova et al
2002
Bt 834



Cry1Be1
AAC32850
Payne et al
1998
Bt PS158C2



Cry1Be2
AAQ52387
Baum et al
2003



Cry1Be3
FJ716102
Xiaodong Sun et al
2009
Bt
No NCBI link July 09



Cry1Bf1
CAC50778
Arnaut et al
2001



Cry1Bf2
AAQ52380
Baum et al
2003



Cry1Bg1
AAO39720
Wang et al
2002



Cry1Ca1
CAA30396
Honee et al
1988
Bt entomocidus







60.5



Cry1Ca2
CAA31951
Sanchis et al
1989
Bt aizawai 7.29



Cry1Ca3
AAA22343
Feitelson
1993
Bt aizawai PS81I



Cry1Ca4
CAA01886
Van Mellaert et al
1990
Bt entomocidus







HD110



Cry1Ca5
CAA65457
Strizhov
1996
Bt aizawai 7.29



Cry1Ca6
AAF37224
Yu et al
2000
Bt AF-2



Cry1Ca7
AAG50438
Aixing et al
2000
Bt J8



Cry1Ca8
AAM00264
Chen et al
2001
Bt c002



Cry1Ca9
AAL79362
Kao et al
2003
Bt G10-01A



Cry1Ca10
AAN16462
Lin et al
2003
Bt E05-20a



Cry1Ca11
AAX53094
Cai et al
2005
Bt C-33



Cry1Cb1
M97880
Kalman et al
1993
Bt galleriae HD29
DNA sequence only



Cry1Cb2
AAG35409
Song et al
2000
Bt c001



Cry1Cb3
ACD50894
Huang et al
2008
Bt 087



Cry1Cb-
AAX63901
Thammasittirong et
2005
Bt TA476-1
insufficient sequence



like

al



Cry1Da1
CAA38099
Hofte et al
1990
Bt aizawai HD68



Cry1Da2
I76415
Payne & Sick
1997

DNA sequence only



Cry1Db1
CAA80234
Lambert
1993
Bt BTS00349A



Cry1Db2
AAK48937
Li et al
2001
Bt B-Pr-88



Cry1Dc1
ABK35074
Lertwiriyawong et al
2006
Bt JC291



Cry1Ea1
CAA37933
Visser et al
1990
Bt kenyae 4F1



Cry1Ea2
CAA39609
Bosse et al
1990
Bt kenyae



Cry1Ea3
AAA22345
Feitelson
1991
Bt kenyae PS81F



Cry1Ea4
AAD04732
Barboza-Corona et
1998
Bt kenyae LBIT-





al

147



Cry1Ea5
A15535
Botterman et al
1994

DNA sequence only



Cry1Ea6
AAL50330
Sun et al
1999
Bt YBT-032



Cry1Ea7
AAW72936
Huehne et al
2005
Bt JC190



Cry1Ea8
ABX11258
Huang et al
2007
Bt HZM2



Cry1Eb1
AAA22346
Feitelson
1993
Bt aizawai







PS81A2



Cry1Fa1
AAA22348
Chambers et al
1991
Bt aizawai







EG6346



Cry1Fa2
AAA22347
Feitelson
1993
Bt aizawai PS81I



Cry1Fb1
CAA80235
Lambert
1993
Bt BTS00349A



Cry1Fb2
BAA25298
Masuda & Asano
1998
Bt morrisoni







INA67



Cry1Fb3
AAF21767
Song et al
1998
Bt morrisoni



Cry1Fb4
AAC10641
Payne et al
1997



Cry1Fb5
AAO13295
Li et al
2001
Bt B-Pr-88



Cry1Fb6
ACD50892
Huang et al
2008
Bt 012



Cry1Fb7
ACD50893
Huang et al
2008
Bt 087



Cry1Ga1
CAA80233
Lambert
1993
Bt BTS0349A



Cry1Ga2
CAA70506
Shevelev et al
1997
Bt wuhanensis



Cry1Gb1
AAD10291
Kuo & Chak
1999
Bt wuhanensis







HD525



Cry1Gb2
AAO13756
Li et al
2000
Bt B-Pr-88



Cry1Gc
AAQ52381
Baum et al
2003



Cry1Ha1
CAA80236
Lambert
1993
Bt BTS02069AA



Cry1Hb1
AAA79694
Koo et al
1995
Bt morrisoni







BF190



Cry1H-
AAF01213
Srifah et al
1999
Bt JC291
insufficient sequence



like



Cry1Ia1
CAA44633
Tailor et al
1992
Bt kurstaki



Cry1Ia2
AAA22354
Gleave et al
1993
Bt kurstaki



Cry1Ia3
AAC36999
Shin et al
1995
Bt kurstaki HD1



Cry1Ia4
AAB00958
Kostichka et al
1996
Bt AB88



Cry1Ia5
CAA70124
Selvapandiyan
1996
Bt 61



Cry1Ia6
AAC26910
Zhong et al
1998
Bt kurstaki S101



Cry1Ia7
AAM73516
Porcar et al
2000
Bt



Cry1Ia8
AAK66742
Song et al
2001



Cry1Ia9
AAQ08616
Yao et al
2002
Bt Ly30



Cry1Ia10
AAP86782
Espindola et al
2003
Bt thuringiensis



Cry1Ia11
CAC85964
Tounsi et al
2003
Bt kurstaki BNS3



Cry1Ia12
AAV53390
Grossi de Sa et al
2005
Bt



Cry1Ia13
ABF83202
Martins et al
2006
Bt



Cry1Ia14
ACG63871
Liu & Guo
2008
Bt11



Cry1Ia15
FJ617445
Guan Peng et al
2009
Bt E-1B
No NCBI link July








2009



Cry1Ia16
FJ617448
Guan Peng et al
2009
Bt E-1A
No NCBI link July








2009



Cry1Ib1
AAA82114
Shin et al
1995
Bt entomocidus







BP465



Cry1Ib2
ABW88019
Guan et al
2007
Bt PP61



Cry1Ib3
ACD75515
Liu & Guo
2008
Bt GS8



Cry1Ic1
AAC62933
Osman et al
1998
Bt C18



Cry1Ic2
AAE71691
Osman et al
2001



Cry1Id1
AAD44366
Choi
2000



Cry1Ie1
AAG43526
Song et al
2000
Bt BTC007



Cry1If1
AAQ52382
Baum et al
2003



Cry1I-like
AAC31094
Payne et al
1998

insufficient sequence



Cry1I-like
ABG88859
Lin & Fang
2006
Bt ly4a3
insufficient sequence



Cry1Ja1
AAA22341
Donovan
1994
Bt EG5847



Cry1Jb1
AAA98959
Von Tersch &
1994
Bt EG5092





Gonzalez



Cry1Jc1
AAC31092
Payne et al
1998



Cry1Jc2
AAQ52372
Baum et al
2003



Cry1Jd1
CAC50779
Arnaut et al
2001
Bt



Cry1Ka1
AAB00376
Koo et al
1995
Bt morrisoni







BF190



Cry1La1
AAS60191
Je et al
2004
Bt kurstaki K1



Cry1-like
AAC31091
Payne et al
1998

insufficient sequence



Cry2Aa1
AAA22335
Donovan et al
1989
Bt kurstaki



Cry2Aa2
AAA83516
Widner & Whiteley
1989
Bt kurstaki HD1



Cry2Aa3
D86064
Sasaki et al
1997
Bt sotto
DNA sequence only



Cry2Aa4
AAC04867
Misra et al
1998
Bt kenyae HD549



Cry2Aa5
CAA10671
Yu & Pang
1999
Bt SL39



Cry2Aa6
CAA10672
Yu & Pang
1999
Bt YZ71



Cry2Aa7
CAA10670
Yu & Pang
1999
Bt CY29



Cry2Aa8
AAO13734
Wei et al
2000
Bt Dongbei 66



Cry2Aa9
AAO13750
Zhang et al
2000



Cry2Aa10
AAQ04263
Yao et al
2001



Cry2Aa11
AAQ52384
Baum et al
2003



Cry2Aa12
ABI83671
Tan et al
2006
Bt Rpp39



Cry2Aa13
ABL01536
Arango et al
2008
Bt 146-158-01



Cry2Aa14
ACF04939
Hire et al
2008
Bt HD-550



Cry2Ab1
AAA22342
Widner & Whiteley
1989
Bt kurstaki HD1



Cry2Ab2
CAA39075
Dankocsik et al
1990
Bt kurstaki HD1



Cry2Ab3
AAG36762
Chen et al
1999
Bt BTC002



Cry2Ab4
AAO13296
Li et al
2001
Bt B-Pr-88



Cry2Ab5
AAQ04609
Yao et al
2001
Bt ly30



Cry2Ab6
AAP59457
Wang et al
2003
Bt WZ-7



Cry2Ab7
AAZ66347
Udayasuriyan et al
2005
Bt 14-1



Cry2Ab8
ABC95996
Huang et al
2006
Bt WB2



Cry2Ab9
ABC74968
Zhang et al
2005
Bt LLB6



Cry2Ab10
EF157306
Lin et al
2006
Bt LyD



Cry2Ab11
CAM84575
Saleem et al
2007
Bt CMBL-BT1



Cry2Ab12
ABM21764
Lin et al
2007
Bt LyD



Cry2Ab13
ACG76120
Zhu et al
2008
Bt ywc5-4



Cry2Ab14
ACG76121
Zhu et al
2008
Bt Bts



Cry2Ac1
CAA40536
Aronson
1991
Bt shanghai S1



Cry2Ac2
AAG35410
Song et al
2000



Cry2Ac3
AAQ52385
Baum et al
2003



Cry2Ac4
ABC95997
Huang et al
2006
Bt WB9



Cry2Ac5
ABC74969
Zhang et al
2005



Cry2Ac6
ABC74793
Xia et al
2006
Bt wuhanensis



Cry2Ac7
CAL18690
Saleem et al
2008
Bt SBSBT-1



Cry2Ac8
CAM09325
Saleem et al
2007
Bt CMBL-BT1



Cry2Ac9
CAM09326
Saleem et al
2007
Bt CMBL-BT2



Cry2Ac10
ABN15104
Bai et al
2007
Bt QCL-1



Cry2Ac11
CAM83895
Saleem et al
2007
Bt HD29



Cry2Ac12
CAM83896
Saleem et al
2007
Bt CMBL-BT3



Cry2Ad1
AAF09583
Choi et al
1999
Bt BR30



Cry2Ad2
ABC86927
Huang et al
2006
Bt WB10



Cry2Ad3
CAK29504
Saleem et al
2006
Bt 5_2AcT(1)



Cry2Ad4
CAM32331
Saleem et al
2007
Bt CMBL-BT2



Cry2Ad5
CAO78739
Saleem et al
2007
Bt HD29



Cry2Ae1
AAQ52362
Baum et al
2003



Cry2Af1
ABO30519
Beard et al
2007
Bt C81



Cry2Ag
ACH91610
Zhu et al
2008
Bt JF19-2



Cry2Ah
EU939453
Zhang et al
2008
Bt
No NCBI link July 09



Cry2Ah2
ACL80665
Zhang et al
2009
Bt BRC-ZQL3



Cry2Ai
FJ788388
Udayasuriyan et al
2009
Bt
No NCBI link July 09



Cry3Aa1
AAA22336
Herrnstadt et al
1987
Bt san diego



Cry3Aa2
AAA22541
Sekar et al
1987
Bt tenebrionis



Cry3Aa3
CAA68482
Hofte et al
1987



Cry3Aa4
AAA22542
McPherson et al
1988
Bt tenebrionis



Cry3Aa5
AAA50255
Donovan et al
1988
Bt morrisoni







EG2158



Cry3Aa6
AAC43266
Adams et al
1994
Bt tenebrionis



Cry3Aa7
CAB41411
Zhang et al
1999
Bt 22



Cry3Aa8
AAS79487
Gao and Cai
2004
Bt YM-03



Cry3Aa9
AAW05659
Bulla and Candas
2004
Bt UTD-001



Cry3Aa10
AAU29411
Chen et al
2004
Bt 886



Cry3Aa11
AAW82872
Kurt et al
2005
Bt tenebrionis







Mm2



Cry3Aa12
ABY49136
Sezen et al
2008
Bt tenebrionis



Cry3Ba1
CAA34983
Sick et al
1990
Bt tolworthi 43F



Cry3Ba2
CAA00645
Peferoen et al
1990
Bt PGSI208



Cry3Bb1
AAA22334
Donovan et al
1992
Bt EG4961



Cry3Bb2
AAA74198
Donovan et al
1995
Bt EG5144



Cry3Bb3
I15475
Peferoen et al
1995

DNA sequence only



Cry3Ca1
CAA42469
Lambert et al
1992
Bt kurstaki







BtI109P



Cry4Aa1
CAA68485
Ward & Ellar
1987
Bt israelensis



Cry4Aa2
BAA00179
Sen et al
1988
Bt israelensis







HD522



Cry4Aa3
CAD30148
Berry et al
2002
Bt israelensis



Cry4A-
AAY96321
Mahalakshmi et al
2005
Bt LDC-9
insufficient sequence



like



Cry4Ba1
CAA30312
Chungjatpornchai et
1988
Bt israelensis





al

4Q2-72



Cry4Ba2
CAA30114
Tungpradubkul et al
1988
Bt israelensis



Cry4Ba3
AAA22337
Yamamoto et al
1988
Bt israelensis



Cry4Ba4
BAA00178
Sen et al
1988
Bt israelensis







HD522



Cry4Ba5
CAD30095
Berry et al
2002
Bt israelensis



Cry4Ba-
ABC47686
Mahalakshmi et al
2005
Bt LDC-9
insufficient sequence



like



Cry4Ca1
EU646202
Shu et al
2008

No NCBI link July 09



Cry4Cb1
FJ403208
Jun & Furong
2008
Bt HS18-1
No NCBI link July 09



Cry4Cb2
FJ597622
Jun & Furong
2008
Bt Ywc2-8
No NCBI link July 09



Cry4Cc1
FJ403207
Jun & Furong
2008
Bt MC28
No NCBI link July 09



Cry5Aa1
AAA67694
Narva et al
1994
Bt darmstadiensis







PS17



Cry5Ab1
AAA67693
Narva et al
1991
Bt darmstadiensis







PS17



Cry5Ac1
I34543
Payne et al
1997

DNA sequence only



Cry5Ad1
ABQ82087
Lenane et al
2007
Bt L366



Cry5Ba1
AAA68598
Foncerrada & Narva
1997
Bt PS86Q3



Cry5Ba2
ABW88932
Guo et al
2008
YBT 1518



Cry6Aa1
AAA22357
Narva et al
1993
Bt PS52A1



Cry6Aa2
AAM46849
Bai et al
2001
YBT 1518



Cry6Aa3
ABH03377
Jia et al
2006
Bt 96418



Cry6Ba1
AAA22358
Narva et al
1991
Bt PS69D1



Cry7Aa1
AAA22351
Lambert et al
1992
Bt galleriae







PGSI245



Cry7Ab1
AAA21120
Narva & Fu
1994
Bt dakota HD511



Cry7Ab2
AAA21121
Narva & Fu
1994
Bt kumamotoensis







867



Cry7Ab3
ABX24522
Song et al
2008
Bt WZ-9



Cry7Ab4
EU380678
Shu et al
2008
Bt
No NCBI link July 09



Cry7Ab5
ABX79555
Aguirre-Arzola et al
2008
Bt monterrey GM-







33



Cry7Ab6
ACI44005
Deng et al
2008
Bt HQ122



Cry7Ab7
FJ940776
Wang et al
2009

No NCBI link Sept 09



Cry7Ab8
GU145299
Feng Jing
2009

No NCBI link Nov 09



Cry7Ba1
ABB70817
Zhang et al
2006
Bt huazhongensis



Cry7Ca1
ABR67863
Gao et al
2007
Bt BTH-13



Cry7Da1
ACQ99547
Yi et al
2009
Bt LH-2



Cry8Aa1
AAA21117
Narva & Fu
1992
Bt kumamotoensis



Cry8Ab1
EU044830
Cheng et al
2007
Bt B-JJX
No NCBI link July 09



Cry8Ba1
AAA21118
Narva & Fu
1993
Bt kumamotoensis



Cry8Bb1
CAD57542
Abad et al
2002



Cry8Bc1
CAD57543
Abad et al
2002



Cry8Ca1
AAA21119
Sato et al.
1995
Bt japonensis







Buibui



Cry8Ca2
AAR98783
Shu et al
2004
Bt HBF-1



Cry8Ca3
EU625349
Du et al
2008
Bt FTL-23
No NCBI link July 09



Cry8Da1
BAC07226
Asano et al
2002
Bt galleriae



Cry8Da2
BD133574
Asano et al
2002
Bt
DNA sequence only



Cry8Da3
BD133575
Asano et al
2002
Bt
DNA sequence only



Cry8Db1
BAF93483
Yamaguchi et al
2007
Bt BBT2-5



Cry8Ea1
AAQ73470
Fuping et al
2003
Bt 185



Cry8Ea2
EU047597
Liu et al
2007
Bt B-DLL
No NCBI link July 09



Cry8Fa1
AAT48690
Shu et al
2004
Bt 185
also AAW81032



Cry8Ga1
AAT46073
Shu et al
2004
Bt HBF-18



Cry8Ga2
ABC42043
Yan et al
2008
Bt 145



Cry8Ga3
FJ198072
Xiaodong et al
2008
Bt FCD114
No NCBI link July 09



Cry8Ha1
EF465532
Fuping et al
2006
Bt 185
No NCBI link July 09



Cry8Ia1
EU381044
Yan et al
2008
Bt su4
No NCBI link July 09



Cry8Ja1
EU625348
Du et al
2008
Bt FPT-2
No NCBI link July 09



Cry8Ka1
FJ422558
Quezado et al
2008

No NCBI link July 09



Cry8Ka2
ACN87262
Noguera & Ibarra
2009
Bt kenyae



Cry8-like
FJ770571
Noguera & Ibarra
2009
Bt canadensis
DNA sequence only



Cry8-like
ABS53003
Mangena et al
2007
Bt



Cry9Aa1
CAA41122
Shevelev et al
1991
Bt galleriae



Cry9Aa2
CAA41425
Gleave et al
1992
Bt DSIR517



Cry9Aa3
GQ249293
Su et al
2009
Bt SC5(D2)
No NCBI link July 09



Cry9Aa4
GQ249294
Su et al
2009
Bt T03C001
No NCBI link July 09



Cry9Aa
AAQ52376
Baum et al
2003

incomplete sequence



like



Cry9Ba1
CAA52927
Shevelev et al
1993
Bt galleriae



Cry9Bb1
AAV28716
Silva-Werneck et al
2004
Bt japonensis



Cry9Ca1
CAA85764
Lambert et al
1996
Bt tolworthi



Cry9Ca2
AAQ52375
Baum et al
2003



Cry9Da1
BAA19948
Asano
1997
Bt japonensis







N141



Cry9Da2
AAB97923
Wasano & Ohba
1998
Bt japonensis



Cry9Da3
GQ249295
Su et al
2009
Bt T03B001
No NCBI link July 09



Cry9Da4
GQ249297
Su et al
2009
Bt T03B001
No NCBI link July 09



Cry9Db1
AAX78439
Flannagan & Abad
2005
Bt kurstaki







DP1019



Cry9Ea1
BAA34908
Midoh & Oyama
1998
Bt aizawai SSK-







10



Cry9Ea2
AAO12908
Li et al
2001
Bt B-Hm-16



Cry9Ea3
ABM21765
Lin et al
2006
Bt lyA



Cry9Ea4
ACE88267
Zhu et al
2008
Bt ywc5-4



Cry9Ea5
ACF04743
Zhu et al
2008
Bts



Cry9Ea6
ACG63872
Liu & Guo
2008
Bt 11



Cry9Ea7
FJ380927
Sun et al
2008

No NCBI link July 09



Cry9Ea8
GQ249292
Su et al
2009
GQ249292
No NCBI link July 09



Cry9Eb1
CAC50780
Arnaut et al
2001



Cry9Eb2
GQ249298
Su et al
2009
Bt T03B001
No NCBI link July 09



Cry9Ec1
AAC63366
Wasano et al
2003
Bt galleriae



Cry9Ed1
AAX78440
Flannagan & Abad
2005
Bt kurstaki







DP1019



Cry9Ee1
GQ249296
Su et al
2009
Bt T03B001
No NCBI link Aug 09



Cry9-like
AAC63366
Wasano et al
1998
Bt galleriae
insufficient sequence



Cry10Aa1
AAA22614
Thorne et al
1986
Bt israelensis



Cry10Aa2
E00614
Aran & Toomasu
1996
Bt israelensis
DNA sequence only







ONR-60A



Cry10Aa3
CAD30098
Berry et al
2002
Bt israelensis



Cry10A-
DQ167578
Mahalakshmi et al
2006
Bt LDC-9
incomplete sequence



like



Cry11Aa1
AAA22352
Donovan et al
1988
Bt israelensis



Cry11Aa2
AAA22611
Adams et al
1989
Bt israelensis



Cry11Aa3
CAD30081
Berry et al
2002
Bt israelensis



Cry11Aa-
DQ166531
Mahalakshmi et al
2007
Bt LDC-9
incomplete sequence



like



Cry11Ba1
CAA60504
Delecluse et al
1995
Bt jegathesan 367



Cry11Bb1
AAC97162
Orduz et al
1998
Bt medellin



Cry12Aa1
AAA22355
Narva et al
1991
Bt PS33F2



Cry13Aa1
AAA22356
Narva et al
1992
Bt PS63B



Cry14Aa1
AAA21516
Narva et al
1994
Bt sotto PS80JJ1



Cry15Aa1
AAA22333
Brown & Whiteley
1992
Bt thompsoni



Cry16Aa1
CAA63860
Barloy et al
1996
Cb malaysia CH18



Cry17Aa1
CAA67841
Barloy et al
1998
Cb malaysia CH18



Cry18Aa1
CAA67506
Zhang et al
1997
Paenibacillus







popilliae



Cry18Ba1
AAF89667
Patel et al
1999
Paenibacillus







popilliae



Cry18Ca1
AAF89668
Patel et al
1999
Paenibacillus







popilliae



Cry19Aa1
CAA68875
Rosso & Delecluse
1996
Bt jegathesan 367



Cry19Ba1
BAA32397
Hwang et al
1998
Bt higo



Cry20Aa1
AAB93476
Lee & Gill
1997
Bt fukuokaensis



Cry20Ba1
ACS93601
Noguera & Ibarra
2009
Bt higo LBIT-976



Cry20-like
GQ144333
Yi et al
2009
Bt Y-5
DNA sequence only



Cry21Aa1
I32932
Payne et al
1996

DNA sequence only



Cry21Aa2
I66477
Feitelson
1997

DNA sequence only



Cry21Ba1
BAC06484
Sato & Asano
2002
Bt roskildiensis



Cry22Aa1
I34547
Payne et al
1997

DNA sequence only



Cry22Aa2
CAD43579
Isaac et al
2002
Bt



Cry22Aa3
ACD93211
Du et al
2008
Bt FZ-4



Cry22Ab1
AAK50456
Baum et al
2000
Bt EG4140



Cry22Ab2
CAD43577
Isaac et al
2002
Bt



Cry22Ba1
CAD43578
Isaac et al
2002
Bt



Cry23Aa1
AAF76375
Donovan et al
2000
Bt
Binary with Cry37Aa1



Cry24Aa1
AAC61891
Kawalek and Gill
1998
Bt jegathesan



Cry24Ba1
BAD32657
Ohgushi et al
2004
Bt sotto



Cry24Ca1
CAJ43600
Beron & Salerno
2005
Bt FCC-41



Cry25Aa1
AAC61892
Kawalek and Gill
1998
Bt jegathesan



Cry26Aa1
AAD25075
Wojciechowska et
1999
Bt finitimus B-





al

1166



Cry27Aa1
BAA82796
Saitoh
1999
Bt higo



Cry28Aa1
AAD24189
Wojciechowska et al
1999
Bt finitimus B-







1161



Cry28Aa2
AAG00235
Moore and Debro
2000
Bt finitimus



Cry29Aa1
CAC80985
Delecluse et al
2000
Bt medellin



Cry30Aa1
CAC80986
Delecluse et al
2000
Bt medellin



Cry30Ba1
BAD00052
Ito et al
2003
Bt entomocidus



Cry30Ca1
BAD67157
Ohgushi et al
2004
Bt sotto



Cry30Ca2
ACU24781
Sun and Park
2009
Bt jegathesan 367



Cry30Da1
EF095955
Shu et al
2006
Bt Y41
No NCBI link July09



Cry30Db1
BAE80088
Kishida et al
2006
Bt aizawai BUN1-







14



Cry30Ea1
ACC95445
Fang et al
2007
Bt S2160-1



Cry30Ea2
FJ499389
Jun et al
2008
Bt Ywc2-8
No NCBI link July09



Cry30Fa1
ACI22625
Tan et al
2008
Bt MC28



Cry30Ga1
ACG60020
Zhu et al
2008
Bt HS18-1



Cry31Aa1
BAB11757
Saitoh & Mizuki
2000
Bt 84-HS-1-11



Cry31Aa2
AAL87458
Jung and Cote
2000
Bt M15



Cry31Aa3
BAE79808
Uemori et al
2006
Bt B0195



Cry31Aa4
BAF32571
Yasutake et al
2006
Bt 79-25



Cry31Aa5
BAF32572
Yasutake et al
2006
Bt 92-10



Cry31Ab1
BAE79809
Uemori et al
2006
Bt B0195



Cry31Ab2
BAF32570
Yasutake et al
2006
Bt 31-5



Cry31Ac1
BAF34368
Yasutake et al
2006
Bt 87-29



Cry32Aa1
AAG36711
Balasubramanian et
2001
Bt yunnanensis





al



Cry32Ba1
BAB78601
Takebe et al
2001
Bt



Cry32Ca1
BAB78602
Takebe et al
2001
Bt



Cry32Da1
BAB78603
Takebe et al
2001
Bt



Cry33Aa1
AAL26871
Kim et al
2001
Bt dakota



Cry34Aa1
AAG50341
Ellis et al
2001
Bt PS80JJ1
Binary with Cry35Aa1



Cry34Aa2
AAK64560
Rupar et al
2001
Bt EG5899
Binary with Cry35Aa2



Cry34Aa3
AAT29032
Schnepf et al
2004
Bt PS69Q
Binary with Cry35Aa3



Cry34Aa4
AAT29030
Schnepf et al
2004
Bt PS185GG
Binary with Cry35Aa4



Cry34Ab1
AAG41671
Moellenbeck et al
2001
Bt PS149B1
Binary with Cry35Ab1



Cry34Ac1
AAG50118
Ellis et al
2001
Bt PS167H2
Binary with Cry35Ac1



Cry34Ac2
AAK64562
Rupar et al
2001
Bt EG9444
Binary with Cry35Ab2



Cry34Ac3
AAT29029
Schnepf et al
2004
Bt KR1369
Binary with Cry35Ab3



Cry34Ba1
AAK64565
Rupar et al
2001
Bt EG4851
Binary with Cry35Ba1



Cry34Ba2
AAT29033
Schnepf et al
2004
Bt PS201L3
Binary with Cry35Ba2



Cry34Ba3
AAT29031
Schnepf et al
2004
Bt PS201HH2
Binary with Cry35Ba3



Cry35Aa1
AAG50342
Ellis et al
2001
Bt PS80JJ1
Binary with Cry34Aa1



Cry35Aa2
AAK64561
Rupar et al
2001
Bt EG5899
Binary with Cry34Aa2



Cry35Aa3
AAT29028
Schnepf et al
2004
Bt PS69Q
Binary with Cry34Aa3



Cry35Aa4
AAT29025
Schnepf et al
2004
Bt PS185GG
Binary with Cry34Aa4



Cry35Ab1
AAG41672
Moellenbeck et al
2001
Bt PS149B1
Binary with Cry34Ab1



Cry35Ab2
AAK64563
Rupar et al
2001
Bt EG9444
Binary with Cry34Ac2



Cry35Ab3
AY536891
AAT29024
2004
Bt KR1369
Binary with Cry34Ab3



Cry35Ac1
AAG50117
Ellis et al
2001
Bt PS167H2
Binary with Cry34Ac1



Cry35Ba1
AAK64566
Rupar et al
2001
Bt EG4851
Binary with Cry34Ba1



Cry35Ba2
AAT29027
Schnepf et al
2004
Bt PS201L3
Binary with Cry34Ba2



Cry35Ba3
AAT29026
Schnepf et al
2004
Bt PS201HH2
Binary with Cry34Ba3



Cry36Aa1
AAK64558
Rupar et al
2001
Bt



Cry37Aa1
AAF76376
Donovan et al
2000
Bt
Binary with Cry23Aa



Cry38Aa1
AAK64559
Rupar et al
2000
Bt



Cry39Aa1
BAB72016
Ito et al
2001
Bt aizawai



Cry40Aa1
BAB72018
Ito et al
2001
Bt aizawai



Cry40Ba1
BAC77648
Ito et al
2003
Bun1-14



Cry40Ca1
EU381045
Shu et al
2008
Bt Y41
No NCBI link July09



Cry40Da1
ACF15199
Zhang et al
2008
Bt S2096-2



Cry41Aa1
BAD35157
Yamashita et al
2003
Bt A1462



Cry41Ab1
BAD35163
Yamashita et al
2003
Bt A1462



Cry42Aa1
BAD35166
Yamashita et al
2003
Bt A1462



Cry43Aa1
BAD15301
Yokoyama and
2003
P. lentimorbus





Tanaka

semadara



Cry43Aa2
BAD95474
Nozawa
2004
P. popilliae







popilliae



Cry43Ba1
BAD15303
Yokoyama and
2003
P. lentimorbus





Tanaka

semadara



Cry43-like
BAD15305
Yokoyama and
2003
P. lentimorbus





Tanaka

semadara



Cry44Aa
BAD08532
Ito et al
2004
Bt entomocidus







INA288



Cry45Aa
BAD22577
Okumura et al
2004
Bt 89-T-34-22



Cry46Aa
BAC79010
Ito et al
2004
Bt dakota



Cry46Aa2
BAG68906
Ishikawa et al
2008
Bt A1470



Cry46Ab
BAD35170
Yamagiwa et al
2004
Bt



Cry47Aa
AAY24695
Kongsuwan et al
2005
Bt CAA890



Cry48Aa
CAJ18351
Jones and Berry
2005
Bs IAB59
binary with 49Aa



Cry48Aa2
CAJ86545
Jones and Berry
2006
Bs 47-6B
binary with 49Aa2



Cry48Aa3
CAJ86546
Jones and Berry
2006
Bs NHA15b
binary with 49Aa3



Cry48Ab
CAJ86548
Jones and Berry
2006
Bs LP1G
binary with 49Ab1



Cry48Ab2
CAJ86549
Jones and Berry
2006
Bs 2173
binary with 49Aa4



Cry49Aa
CAH56541
Jones and Berry
2005
Bs IAB59
binary with 48Aa



Cry49Aa2
CAJ86541
Jones and Berry
2006
Bs 47-6B
binary with 48Aa2



Cry49Aa3
CAJ86543
Jones and Berry
2006
BsNHA15b
binary with 48Aa3



Cry49Aa4
CAJ86544
Jones and Berry
2006
Bs 2173
binary with 48Ab2



Cry49Ab1
CAJ86542
Jones and Berry
2006
Bs LP1G
binary with 48Ab1



Cry50Aa1
BAE86999
Ohgushi et al
2006
Bt sotto



Cry51Aa1
ABI14444
Meng et al
2006
Bt F14-1



Cry52Aa1
EF613489
Song et al
2007
Bt Y41
No NCBI link July09



Cry52Ba1
FJ361760
Jun et al
2008
Bt BM59-2
No NCBI link July09



Cry53Aa1
EF633476
Song et al
2007
Bt Y41
No NCBI link July09



Cry53Ab1
FJ361759
Jun et al
2008
Bt MC28
No NCBI link July09



Cry54Aa1
ACA52194
Tan et al
2009
Bt MC28



Cry55Aa1
ABW88931
Guo et al
2008
YBT 1518



Cry55Aa2
AAE33526
Bradfisch et al
2000
BT Y41



Cry56Aa1
FJ597621
Jun & Furong
2008
Bt Ywc2-8
No NCBI link July09



Cry56Aa2
GQ483512
Guan Peng et al
2009
Bt G7-1
No NCBI link Aug09



Cry57Aa1
ANC87261
Noguera & Ibarra
2009
Bt kim



Cry58Aa1
ANC87260
Noguera & Ibarra
2009
Bt entomocidus



Cry59Aa1
ACR43758
Noguera & Ibarra
2009
Bt kim LBIT-980


















Vip3Aa1
Vip3Aa
AAC37036
Estruch et al
1996
PNAS 93,
AB88








5389-5394


Vip3Aa2
Vip3Ab
AAC37037
Estruch et al
1996
PNAS 93,
AB424







5389-5394


Vip3Aa3
Vip3Ac

Estruch et al
2000
U.S. Pat. No. 6,137,033







October 2000


Vip3Aa4
PS36A Sup
AAR81079
Feitelson et al
1998
U.S. Pat. No. 6,656,908
Bt PS36A
WO9818932(A2,







December 2003

A3) 7 May









1998


Vip3Aa5
PS81F Sup
AAR81080
Feitelson et al
1998
U.S. Pat. No. 6,656,908
Bt PS81F
WO9818932(A2,







December 2003

A3) 7 May









1998


Vip3Aa6
Jav90 Sup
AAR81081
Feitelson et al
1998
U.S. Pat. No. 6,656,908
Bt
WO9818932(A2,







December 2003

A3) 7 May









1998


Vip3Aa7
Vip83
AAK95326
Cai et al
2001
unpublished
Bt YBT-833


Vip3Aa8
Vip3A
AAK97481
Loguercio et al
2001
unpublished
Bt HD125


Vip3Aa9
VipS
CAA76665
Selvapandiyan
2001
unpublished
Bt A13





et al


Vip3Aa10
Vip3V
AAN60738
Doss et al
2002
Protein Expr.
Bt







Purif. 26, 82-88


Vip3Aa11
Vip3A
AAR36859
Liu et al
2003
unpublished
Bt C9


Vip3Aa12
Vip3A-WB5
AAM22456
Wu and Guan
2003
unpublished
Bt


Vip3Aa13
Vip3A
AAL69542
Chen et al
2002
Sheng Wu
Bt S184







Gong Cheng







Xue Bao 18,







687-692


Vip3Aa14
Vip
AAQ12340
Polumetla et al
2003
unpublished
Bt tolworthi


Vip3Aa15
Vip3A
AAP51131
Wu et al
2004
unpublished
Bt WB50


Vip3Aa16
Vip3LB
AAW65132
Mesrati et al
2005
FEMS Micro
Bt







Lett 244,







353-358


Vip3Aa17
Jav90

Feitelson et al
1999
U.S. Pat. No. 6,603,063
Javelin 1990
WO9957282(A2,







August 2003

A3) 11 Nov









1999


Vip3Aa18

AAX49395
Cai and Xiao
2005
unpublished
Bt 9816C


Vip3Aa19
Vip3ALD
DQ241674
Liu et al
2006
unpublished
Bt AL


Vip3Aa19
Vip3A-1
DQ539887
Hart et al
2006
unpublished


Vip3Aa20
Vip3A-2
DQ539888
Hart et al
2006
unpublished


Vip3Aa21
Vip
ABD84410
Panbangred
2006
unpublished
Bt aizawai


Vip3Aa22
Vip3A-LS1
AAY41427
Lu et al
2005
unpublished
Bt LS1


Vip3Aa23
Vip3A-LS8
AAY41428
Lu et al
2005
unpublished
Bt LS8


Vip3Aa24

BI 880913
Song et al
2007
unpublished
Bt WZ-7


Vip3Aa25

EF608501
Hsieh et al
2007
unpublished


Vip3Aa26

EU294496
Shen and Guo
2007
unpublished
Bt TF9


Vip3Aa27

EU332167
Shen and Guo
2007
unpublished
Bt 16


Vip3Aa28

FJ494817
Xiumei Yu
2008
unpublished
Bt JF23-8


Vip3Aa29

FJ626674
Xieumei et al
2009
unpublished
Bt JF21-1


Vip3Aa30

FJ626675
Xieumei et al
2009
unpublished
MD2-1


Vip3Aa31

FJ626676
Xieumei et al
2009
unpublished
JF21-1


Vip3Aa32

FJ626677
Xieumei et al
2009
unpublished
MD2-1


—



—


Vip3Ab1
Vip3B
AAR40284
Feitelson et al
1999
U.S. Pat. No. 6,603,063
Bt KB59A4-6
WO9957282(A2,







August 2003

A3) 11 Nov









1999


Vip3Ab2
Vip3D
AAY88247
Feng and Shen
2006
unpublished
Bt


—



—


Vip3Ac1
PS49C

Narva et al
—
US







application







20040128716


—



—


Vip3Ad1
PS158C2

Narva et al
—
US







application







20040128716


Vip3Ad2
ISP3B
CAI43276
Van Rie et al
2005
unpublished
Bt


—



—


Vip3Ae1
ISP3C
CAI43277
Van Rie et al
2005
unpublished
Bt


—



—


Vip3Af1
ISP3A
CAI43275
Van Rie et al
2005
unpublished
Bt


Vip3Af2
Vip3C
ADN08753
Syngenta
—
WO







03/075655


—



—


Vip3Ag1
Vip3B
ADN08758
Syngenta
—
WO







02/078437


Vip3Ag2

FJ556803
Audtho et al
2008

Bt


—



—


Vip3Ah1
Vip3S
DQ832323
Li and Shen
2006
unpublished
Bt


—


Vip3Ba1

AAV70653
Rang et al
2004
unpublished


—


Vip3Bb1
Vip3Z
ADN08760
Syngenta
—
WO







03/075655


Vip3Bb2

EF439819
Akhurst et al
2007

## Claims

1. A transgenic plant comprising DNA encoding a Cry1Ab insecticidal protein, DNA encoding a Cry1Be insecticidal protein, and DNA encoding a Cry1Fa insecticidal protein, wherein said Cry1Be insecticidal protein comprises SEQ ID NO:1 and said Cry1Ab insecticidal protein comprises SEQ ID NO:2, and wherein said Cry1Ab and said Cry1Be insecticidal proteins have different receptor binding sites in fall armyworm gut.

2. The transgenic plant of claim 1, said plant further comprising DNA encoding a fourth insecticidal protein selected from the group consisting of Cry2A, Cry1I, DIG-3, Vip3Ab, Cry1Ca and Cry1E.

3. A seed of the plant of claim 1.

4. A plurality of plants comprising non-Bt refuge plants and plants of claim 1, wherein said refuge plants comprise less than 40% of said plurality of plants.

5. The plurality of plants of claim 4, wherein said refuge plants comprise less than 30% of said plurality of plants.

6. The plurality of plants of claim 4, wherein said refuge plants comprise less than 20% of said plurality of plants.

7. The plurality of plants of claim 4, wherein said refuge plants comprise less than 10% of said plurality of plants.

8. The plurality of plants of claim 4, wherein said refuge plants comprise less than 5% of said plurality of plants.

9. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of the seeds of claim 3, wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.

10. The mixture of seeds of claim 9, wherein said refuge seeds comprise less than 30% of all the seeds in the mixture.

11. The mixture of seeds of claim 9, wherein said refuge seeds comprise less than 20% of all the seeds in the mixture.

12. The mixture of seeds of claim 9, wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.

13. The mixture of seeds of claim 9, wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.

14. A method of managing development of resistance to a Cry protein by a fall armyworm, said method comprising planting seeds to produce the plurality of plants of claim 4.

15. The method of claim 14, wherein said seeds are planted so that the resulting plants occupy more than 10 acres.

16. The plant of claim 1, wherein said plant is selected from the group consisting of corn, soybeans, and cotton.

17. The plant of claim 16, wherein said plant is a corn plant.

18. A plant cell of the plant of claim 1, wherein said plant cell comprises said DNA encoding said Cry1Be insecticidal protein, said DNA encoding said Cry1Ab insecticidal protein, and said DNA encoding a Cry1Fa insecticidal protein.

19. A method of controlling a fall armyworm insect, said method comprising plating a seed comprising DNA encoding a Cry1Be insecticidal protein, a Cry1Ab insecticidal protein and a Cry1Fa insecticidal protein, wherein said Cry1Ab and said Cry1Be insecticidal proteins have different receptor binding sites in fall armyworm gut.