# Patent

## Title
Mutant forms of cholera holotoxin as an adjuvant

## Abstract

Mutant cholera holotoxins comprising a cholera toxin subunit A having single amino acid substitutions in the amino acid positions 16 or 72 or a double amino acid substitution in the amino acid positions 16 and 68 or 68 and 72 have reduced toxicity compared to the wild-type cholera holotoxin. The mutant cholera holotoxins are useful as adjuvants in immunogenic compositions to enhance the immune response in a vertebrate host to a selected antigen from a pathogenic bacterium, virus, fungus, or parasite, a cancer cell, a tumor cell, an allergen, or a self-molecule.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a division of U.S. patent application Ser. No. 10/478,307, filed Dec. 4, 2003 now U.S. Pat. No. 7,285,281, which is a national stage of PCT/US02/20978, filed Jun. 5, 2002, which claims the benefit of the priority under 35 USC 119(e) of US Provisional Patent Application No. 60/296,537, filed Jun. 7, 2001.


BACKGROUND OF THE INVENTION
The body's immune system activates a variety of mechanisms for attacking pathogens (Janeway, Jr, C A. and Travers P., eds., in Immunobiology, “The Immune System in Health and Disease,” Second Edition, Current Biology Ltd., London, Great Britain (1996)). However, not all of these mechanisms are necessarily activated after immunization. Protective immunity induced by immunization is dependent on the capacity of an immunogenic composition to elicit the appropriate immune response to resist or eliminate the pathogen. Depending on the pathogen, this may require a cell-mediated and/or humoral immune response.
Many antigens are poorly immunogenic or non-immunogenic when administered by themselves. Strong adaptive immune responses to antigens almost always require that the antigens be administered together with an adjuvant, a substance that enhances the immune response (Audbert, F. M. and Lise, L. D. 1993 Immunology Today, 14: 281-284).
The need for effective immunization procedures is particularly acute with respect to infectious organisms that cause acute infections at, or gain entrance to the body through, the gastrointestinal, pulmonary, nasopharyngeal or genitourinary surfaces. These areas are bathed in mucus, which contains immunoglobulins consisting largely of secretory immunoglobulin IgA (Hanson, L. A., 1961 Intl. Arch. Allergy Appl. Immunol., 18, 241-267; Tomasi T. B., and Zigelbaum, S., 1963 J. Clin. Invest., 42, 1552-1560; Tomasi, T. B., et al., 1965 J. Exptl. Med., 121, 101-124). This immunoglobulin is derived from large numbers of IgA-producing plasma cells, which infiltrate the lamina propria regions underlying the mucosal membranes (Brandtzaeg, P., and Baklein, K, 1976 Scand. J. Gastroenterol., 11 (Suppl. 36), 1-45; and Brandtzaeg, P., 1984 “Immune Functions of Human Nasal Mucosa and Tonsils in Health and Disease”, page 28 et seq. in Immunology of the Lung and Upper Respiratory Tract, Bienenstock, J., ed., McGraw-Hill, New York, N.Y.). The secretory immunoglobulin IgA is specifically transported to the luminal surface through the action of the secretory component (Solari, R, and Kraehenbuhl, J-P, 1985 Immunol. Today, 6, 17-20).
Parenteral immunization regimens are usually ineffective in inducing secretory IgA responses. Secretory immunity is most often achieved through the direct immunization of mucosally associated lymphoid tissues. Following their induction at one mucosal site, the precursors of IgA-producing plasma cells extravasate and disseminate to diverse mucosal tissues where final differentiation to high-rate IgA synthesis occurs (Crabbe, P. A., et al., 1969 J. Exptl. Med., 130, 723-744; Bazin, H., et al., 1970 J. Immunol., 105, 1049-1051; Craig, S. W., and Cebra, J. J., 1971 J. Exptl. Med., 134, 188-200). Extensive studies have demonstrated the feasibility of mucosal immunization to induce this common mucosal immune system (Mestecky, J., et al., 1978 J. Clin. Invest., 61, 731-737), but with rare exceptions the large doses of antigen required to achieve effective immunization have made this approach impractical for purified antigens.
Among the strategies investigated to overcome this problem is the use of mucosal adjuvants. A number of adjuvants that enhance the immune response of antigens are known in the prior art (Elson, C. O., and Ealding, W., 1984 J. Immunol., 132, 2736-2741). These adjuvants, when mixed with an antigen, render the antigen particulate, helping retain the antigen in the body for longer periods of time, thereby promoting increased macrophage uptake and enhancing immune response. However, untoward reactions elicited by many adjuvants or their ineffectiveness in inducing mucosal immunity have necessitated the development of better adjuvants for delivery of immunogenic compositions. Unfortunately, adjuvant development to date has been largely an empirical exercise (Janeway, Jr., et al, cited above at pages 12-25 to 12-35). Thus, a rational and a more direct approach is needed to develop effective adjuvants for delivery of antigenic compositions.
It has been reported that the toxin secreted by the Gram-negative bacterium Vibrio cholerae (V. cholerae), the causative agent of the gastrointestinal disease cholera, is extremely potent as an adjuvant. Cholera toxin (CT) has been reported as a 382 amino acid sequence (SEQ ID NO: 1) (Mekalanos, J. J., et al., 1983 Nature, 306, 551-557), which has an 18 amino acid signal (amino acids 1 to 18 of SEQ ID NO: 1). The cholera toxin holotoxin molecule is a hexaheteromeric complex that consists of a a peptide subunit designated CT-A (SEQ ID NO: 2 or amino acids 19 to 258 of SEQ ID NO: 1), which is responsible for the enzymatic activity of the toxin, and five identical peptide subunits, each designated CT-B (each having a 21 amino acid signal (amino acids 259 to 379 of SEQ ID NO: 1), followed by the CT-B peptide subunit (amino acids 280-382 of SEQ ID NO: 1)), which is involved in the binding of the toxin to the intestinal epithelial cells as well as other cells which contain ganglioside GM1 on their surface (Gill, D. M., 1976 Biochem., 15, 1242-1248; Cuatrecasas, P., 1973 Biochem., 12, 3558-3566). CT produced by V. cholerae has the CT-A subunit proteolytically cleaved within the single disulfide-linked loop between the cysteines at amino acid positions 187 and 199 of the mature CT-A (SEQ ID NO: 2) to produce an enzymatically active A1 polypeptide (Kassis, S., et al., 1982 J. Biol. Chem., 257, 12148-12152), and a smaller polypeptide A2, which links fragment A1 to the CT-B pentamer (Mekalanos, J. J., et al., 1979 J. Biol. Chem., 254, 5855-5861). Toxicity results when the enzymatically active fragment CT-A1, upon entry into enterocytes, ADP-ribosylates a regulatory G-protein (Gsα). This leads to constitutive activation of adenylate cyclase, increased intracellular concentration of cAMP, and secretion of fluid and electrolytes into the lumen of the small intestine, thereby causing toxicity (Gill, D. M., and Meren, R., 1978 Proc. Natl. Acad. Sci., USA, 75, 3050-3054). In vitro, ADP-ribosyl transferase activity of CT is stimulated by the presence of accessory proteins called ARFs, small GTP-binding proteins known to be involved in vesicle trafficking within the eukaryotic cell (Welsh, C. F., et al., “ADP-Ribosylation Factors: A Family of Guanine Nucleotide-Binding Proteins that Activate Cholera Toxin and Regulate Vesicular Transport”, pages 257-280 in Handbook of Natural Toxins: Bacterial Toxins and Virulence Factors in Disease Vol. 8 (Moss, J., et al., eds., Marcel Dekker, Inc., New York, N.Y. (1995)).
Co-administration of CT with an unrelated antigen has been reported to result in the induction of concurrent circulating and mucosal antibody responses to that antigen (Mekalanos, J. J., et al., 1983 Nature, 306, 551-557). To minimize the occurrence of undesirable symptoms such as diarrhea caused by wild-type CT in humans, it would be preferable to use as an adjuvant a form of the CT holotoxin that has substantially reduced toxicity. Mutants of CT have been suggested as a means for achieving a more useful adjuvant. One way to rationally design mutant cholera toxin holotoxins (CT-CRMs) with substantially reduced toxicity is to identify and alter amino acid residues in the toxin molecule that are completely conserved in the family of cholera (CT) and related heat-labile enterotoxins (LT-I, LT-IIa and LT-IIb) of E. coli. Another rational way to generate mutant CT-CRMs with substantially reduced toxicity is to alter amino acid residues in the holotoxin molecule that have been identified as being important for NAD-binding based on the structural alignment of the CT backbone with the backbone of related toxins possessing ADP-ribosyl transferase enzyme activity such as diphtheria toxin (DT) and pertussis toxin (PT) (Holmes, R. K., “Heat-labile enterotoxins (Escherichia coli)” in Guidebook to Protein Toxins and their Use in Cell Biology, Montecucco, C. and Rappnoli, R., Eds., Oxford Univ. Press, Oxford, England (1997); and Holmes, R. K. et al, “Cholera toxins and related enterotoxins of Gram-negative bacteria”, pp. 225-256 in Handbook of Natural Toxins: Bacterial Toxins and Virulence Factors in Disease, vol. 8, Moss. J., et al, Eds., Marcel Dekker, Inc., New York, N.Y. 1995).
Recently, one such rationally-designed, genetically-detoxified mutant of CT was disclosed wherein a single nonconservative amino acid substitution (glutamic acid to histidine) was introduced by altering the amino acid at position 29 in the A subunit (designated CT-CRME29H). The resulting mutant cholera holotoxin demonstrated substantially reduced enzymatic toxicity, but with superior adjuvanting and immunogenic properties (International Patent Publication No. WO 00/18434, incorporated in its entirety by reference).
Thus, there is a need to identify and/or rationally design additional mutant forms of the CT holotoxin that have substantially reduced toxicity, yet possess the same or enhanced adjuvanting properties as the wild-type CT holotoxin
SUMMARY OF THE INVENTION
In one aspect, this invention provides novel mutant, immunogenic forms of cholera holotoxin (CT-CRMs) having significantly reduced toxicity compared to a wild-type CT, but which retain their ability as powerful stimulators of the immune system Specifically, the invention pertains to four mutant cholera holotoxins (CT-CRMs), desirably generated by site-directed mutagenesis and having substantially reduced toxicity compared to a wild-type CT, but with no loss in adjuvanting properties.
In one embodiment, a novel CT-CRM of this invention comprises the amino acid sequence of CT subunit A or a fragment thereof wherein the amino acid residue in the amino acid position 16 of the A subunit is substituted with another amino acid which substitution results in a substantial reduction in toxicity. In a preferred embodiment of the invention, the amino acid isoleucine at amino acid position 16 of the A subunit is substituted with an alanine. For determination of the amino acid position, the sequence of CT-A is exemplified in SEQ ID NO: 2. However, other variants and fragments of CT-A may also be employed.
In another embodiment, a novel CT-CRM of this invention comprises the amino acid sequence of CT subunit A or a fragment thereof, wherein the amino acid residue in the amino acid position 72 of the A subunit is substituted with another amino acid which substitution results in a substantial reduction in toxicity. In a preferred embodiment of the invention, the amino acid valine at the amino acid position 72 of the A subunit is substituted with a tyrosine.
In another embodiment, a novel immunogenic, mutant CT-CRM of this invention has substantially reduced CT toxicity and comprises the amino acid sequence of subunit A of CT or a fragment thereof wherein both amino acid residues in the amino acid positions 16 and 68 in the A subunit are substituted with amino acids different from that present in amino acid positions 16 and 68 of wild-type CT, which substitutions result in a substantial reduction in toxicity: In a preferred embodiment of this aspect of the invention, the amino acid alanine is substituted for isoleucine at the amino acid position 16 in the A subunit, and the amino acid tyrosine is substituted for serine at amino acid position 68 in the A subunit.
In yet another embodiment, a novel immunogenic, mutant CT-CRM of this invention has substantially reduced CT toxicity and comprises the amino acid sequence of subunit A of CT or a fragment thereof, wherein both amino acid residues in the amino acid positions 68 and 72 in the A subunit are substituted with amino acids different from that present in amino acid positions 68 and 72 of wild-type CT, which substitutions result in a substantial reduction in toxicity. In a preferred embodiment of this aspect of the invention, the amino acid tyrosine is substituted for serine at amino acid position 68 of the A subunit, and the amino acid tyrosine is substituted for valine at amino acid position 72 of the A subunit.
In another aspect, the invention provides a method for producing the novel CT-CRMs described above by employing site-directed mutagenesis of the DNA encoding the A subunit in the wild-type CT using conventional techniques, such that the mutagenized CT now has substantially reduced toxicity-without compromising the toxin's ability to stimulate an immune response.
In yet another aspect of the invention, there is provided an immunogenic composition comprising a selected antigen, a mutant CT-CRM as described above as an adjuvant to enhance the immune response in a vertebrate host to the antigen, and a pharmaceutically acceptable diluent, excipient or carrier. Preferably, the CT-CRM is useful for the generation or enhancement of systemic and/or mucosal antigenic immune responses in a vertebrate host to the selected antigen. The selected antigen may be a polypeptide, peptide or fragment derived from a pathogenic virus, bacterium, fungus or parasite. The selected antigen may be a polypeptide, peptide or fragment derived from a cancer cell or tumor cell. The selected antigen may be a polypeptide, peptide or fragment derived from an allergen so as to interfere with the production of IgE so as to moderate allergic responses to the allergen. The selected antigen may be a polypeptide, peptide or fragment derived from a molecular portion thereof which represents those produced by a host (a self molecule) in an undesired manner, amount or location, such as those from amyloid precursor protein, so as to prevent or treat disease characterized by amyloid deposition in a vertebrate host.
In still another aspect, this invention provides a method for using these CT-CRMs as adjuvants in immunogenic compositions or methods for increasing the ability of an antigenic composition containing a selected antigen as described above to elicit an immune response in vertebrate host by including an effective adjuvanting amount of one or more of the novel detoxified mutant cholera holotoxins (CT-CRMs) described above.
In yet a further aspect of the invention, there are provided DNA sequences encoding the novel immunogenic, mutant CT-CRMs with substantially reduced toxicity as described above. Preferably, the DNA sequence(s) encodes for both the mutant A subunit with reduced toxicity and subunit B. Alternatively, the DNA sequence may encode only the mutant A subunit with reduced toxicity, where the mutant CT-A is fused with an additional binding domain, or is co-expressed with LT-B and allowed to co-assemble.
In a further aspect of the invention, there is provided a plasmid containing isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic, detoxified, mutant cholera holotoxin as described herein, and wherein such a DNA sequence is operatively linked to regulatory sequences which direct expression of the CT-CRM in a host cell. Preferably the regulatory sequences comprise an arabinose inducible promoter. In one embodiment of this aspect, the invention relates to a plasmid, designated pLP903, that contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic mutant CT-CRM with substantially reduced toxicity wherein the amino acid alanine is substituted for isoleucine at amino acid position 16 in the A subunit. In a second embodiment of this aspect, the invention relates to a plasmid, designated pLP905, that contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic mutant CT-CRM with substantially reduced toxicity wherein the amino acid tyrosine is substituted for valine at the amino acid position 72 in the A subunit. In a third embodiment of this aspect, the invention relates to a plasmid, designated pLP904, that contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic, mutant CT-CRM with substantially reduced toxicity wherein the amino acid alanine is substituted for isoleucine at amino acid position 16, and amino acid tyrosine is substituted for serine at amino acid position 68 in the A subunit. In yet an additional embodiment of this aspect, the invention relates to a plasmid, designated pLP906, that contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic, mutant CT-CRM with substantially reduced toxicity wherein the amino acid tyrosine is substituted for serine at the amino acid position 68, and amino acid tyrosine is substituted for valine at amino acid position 72 in the A subunit.
In a further aspect of the invention, there is provided a suitable host cell line transformed, infected, transduced or transfected with a plasmid as described herein. The immunogenic, detoxified, mutant cholera holotoxins are produced by transforming, infecting, transducing or transfecting a suitable host cell with one of the plasmids described above and culturing the host cell under culture conditions which permit the expression by the host cell of said recombinant immunogenic, mutant cholera holotoxin protein with substantially reduced toxicity.
These and other aspects of the invention will be apparent to one of skill in the art upon reading of the following detailed description of the invention


DETAILED DESCRIPTION OF THE INVENTION
Mutant forms of cholera holotoxin that exhibit reduced toxicity, but which retain their superior adjuvanting properties, and the utility of these mutant forms of CTs as adjuvants in immunogenic compositions are described herein.
A. Mutant, Detoxified Cholera Toxin Holotoxins
Novel mutant, detoxified immunogenic forms of cholera holotoxin (CT-CRMs) of this invention are characterized by significantly reduced toxicity compared to a wild-type CT. However, such CT-CRMs retain their ability as powerful stimulators of the immune system The CT-CRMs of this invention are characterized by one or several amino acid substitutions in the mature CT-A subunit of cholera ton. The various mutant CT-A subunits of this invention also retained their ability to assemble with CT-B subunits to form mutant CT holotoxins that resembled wild-type CT in adjuvanticity, but which exhibited substantially reduced toxicity compared to the wild-type CT. The CT-CRM of this invention may employ mutant or altered CT-A subunits associated with wild-type CT-B subunits to create a functional holotoxin Alternatively, the CT-CRMs of this invention may comprise the altered or mutated CT-A subunits associated with altered or mutated CT-B subunits.
For determination of the amino acid position numbers describing the locations of the amino acid substitutions in the CT-CRMs of this invention, the sequence of mature CT-A is exemplified as SEQ ID NO: 2, i.e., amino acids 19-258 of SEQ ID NO: 1, a wild-type CT sequence. The nucleotide sequence encoding the A subunit of the cholera holotoxin is set forth in International patent publication No. WO 93/13202. Similarly, a suitable mature CT-B sequence may be illustrated by amino acids 280-382 of SEQ ID NO: 1. However, other variants, biotypes and fragments of CT-A and CT-B of V. cholerae may also be employed as sequences containing the amino acid substitutions described herein. See, for example, the ELTOR biotype of C. Shi et al, 1993 Sheng Wu Hua Hsueh Tsa Chih, 9(4):395-399; NCBI database locus No. AAC34728, and other sources of variants of V. cholerae toxin.
Preferably, the amino acid substitutions resulting in the CT-CRMs of this invention are the result of replacing one amino acid with another amino acid having similar structural and/or chemical properties, i.e. conservative amino acid replacements. “Conservative” amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved. For example, non-polar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, tryptophan, and methionine; polar/neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid. This invention is exemplified by CT-CRMs having a single amino acid substitution at either amino acid position 16 or at amino acid position 72 or double amino acid substitution at amino acid positions 16 and 68 or 68 and 72, as summarized in Table 1.







TABLE 1







Single and Double CT-CRM Mutants










Amino Acid





Substitution
Native
Mutant
Abbreviation





16
Isoleucine16
Alanine16
CT-CRMI16A


72
Valine72
Tyrosine72
CT-CRMV72Y


16 and 68
Isoleucine16
Alanine16
CT-CRMI16A, S68Y



Serine68
Tyrosine68


68 and 72
Serine68
Tyrosine68
CT-CRMS68Y, V72Y



Valine72
Tyrosine72









Thus, in one embodiment, a novel CT-CRM of this invention comprises the amino acid sequence of CT subunit A or a fragment thereof wherein the amino acid residue in the amino acid position 16 of the A subunit is substituted with another amino acid which substitution results in a substantial reduction in toxicity. In a preferred embodiment of the invention, the amino acid isoleucine at amino acid position 16 of the A subunit is substituted with an alanine. This CT-CRMI16A demonstrates superior adjuvanting properties.
In another embodiment, a novel CT-CRM of this invention comprises the amino acid sequence of CT subunit A or a fragment thereof wherein the amino acid residue in the amino acid position 72 of the A subunit is substituted with another amino acid, which substitution results in a substantial reduction in toxicity. In a preferred embodiment of the invention, the amino acid valine at the amino acid position 72 of the A subunit is substituted with a tyrosine, resulting in CT-CRMV72Y. This CT-CRMV72Y demonstrates superior adjuvanting properties.
In another embodiment, a novel immunogenic, mutant CT-CRM of this invention has substantially reduced CT toxicity and comprises the amino acid sequence of subunit A of CT or a fragment thereof, wherein both amino acid residues in the amino acid positions 16 and 68 in the A subunit are substituted with amino acids different from that present in amino acid positions 16 and 68 of wild-type CT, which substitutions result in a substantial reduction in toxicity. In a preferred embodiment of this aspect of the invention, the amino acid alanine is substituted for isoleucine at the amino acid position 16 in the A subunit, and the amino acid tyrosine is substituted for serine at amino acid position 68 in the A subunit, resulting in CT-CRMI16A, S68Y, which demonstrates superior adjuvanting properties
In yet another embodiment, a novel immunogenic, mutant CT-CRM of this invention has substantially reduced CT toxicity and comprises the amino acid sequence of subunit A of CT or a fragment thereof wherein both amino acid residues in the amino acid positions 68 and 72 in the A subunit are substituted with amino acids different from that present in amino acid positions 68 and 72 of wild-type CT, which substitutions result in a substantial reduction in toxicity. In a preferred embodiment of this aspect of the invention, the amino acid tyrosine is substituted for serine at amino acid position 68 of the A subunit, and the amino acid tyrosine is substituted for valine at amino acid position 72 of the A subunit. This CT-CRMS68Y, V72Y demonstrates superior adjuvanting properties.
The phenotypic effects of the novel CT-CRMs of Table 1 on the structure and function of CT were assessed. The mutant A subunits generated by site directed mutagenesis of the CT-encoding gene were also able to assemble into immunoreactive holotoxin in the presence of subunit B as determined by non-denaturing gel electrophoresis assay (see Table 3, Example 2). Each mutant holotoxin was also tested in a Y-1 adrenal tumor cell assay to determine its residual toxicity compared to wild-type CT holotoxin (see Tables 4 and 5, Example 3). The results presented in Table 4 demonstrate that the mutant CT-CRMs had substantially reduced toxicity when compared with wild-type cholera holotoxin. The residual toxicities of the CT-CRMs with single and double amino acid substitutions were substantially reduced in comparison to that of the wild-type CT.
Each of the mutant CT-CRMs was also compared to wild-type CT in an ADP-ribosyltransferase activity assay (See Example 4). The results, which were generally in agreement with the toxicity data generated in the Y-1 adrenal cell assay, indicated that the ADP-ribosyltransferase activity of the various CT-CRMs was substantially diminished when compared to wild-type CT (Table 6). The mutant with the largest ADP-ribosyl-transferase activity appeared to be the double mutant CT-CRMI1A,S68Y. This activity was approximately only 3.3% of wild-type CT. The enzyme activity of CT-CRMs. CT-CRMV72Y, CT-CRMI16A, and CT-CRMS68Y,V72Y, were 1.1%, 2.4% and 1.2% respectively of the activity of the wild-type CT.
Still other CT-CRMs of this invention may contain at least the single or double mutations described specifically above and at least one additional mutation at a position other than at one or more of the amino acid residues 16, 68, or 72 as set forth above. International patent publication No. WO 93/13202, which is hereby incorporated by reference, describes a series of mutations in the CT-A subunit that serve to reduce the toxicity of the cholera holotoxin. These mutations include making substitutions for the arginine at amino acid 7, the aspartic acid at position 9, the arginine at position 11, the glutamic acid at position 29, the histidine at position 44, the valine at position 53, the arginine at position 54, the serine at position 61, the serine at position 63, the histidine at position 70, the valine at position 97, the tyrosine at position 104, the proline at position 106, the histidine at position 107, the glutamic acid at position 110, the glutamic acid at position 112, the serine at position 114, the tryptophan at position 127, the arginine at position 146 and the arginine at position 192. International patent publication No. WO 98/42375, which is hereby incorporated by reference, describes making a substitution for the serine at amino acid 109 in the A subunit, which serves to reduce the toxicity of the cholera holotoxin.
Other useful CT-CRM mutant proteins useful in this invention include a full-length holotoxin with one or more of the specific mutations provided above, a polypeptide or a fragment thereof containing the mutagenized residues described above and which protein, polypeptide or fragment retains the adjuvanticity of wild-type CT from which it is derived, but is characterized by reduced toxicity.
Immunologically active fragments of these CT-CRMs with reduced enzymatic activity may also be useful in the methods and compositions of this invention. Fragments ordinarily will contain at least at least about 25 contiguous amino acids of the CT-CRM proteins containing the site of mutagenesis noted above. More typically a CT-CRM fragment contains at least about 75 contiguous amino acids. Another fragment of a CT-CRM contains at least about 100 contiguous amino acids. Still another embodiment of a CT-CRM subunit A contains at least about 150 contiguous amino acids in length.
A fragment of the CT-CRMs described herein is useful in the methods and compositions described below if it generates or enhances the immune response to selected antigens in the vertebrate host. Fragments include truncations of the carboxy-terminal region of the CT-CRMs. For example, a CT-CRM truncated so that it contains only a CT-A mutant subunit is a desirable fragment. Similarly, CT-A subunits truncated at about residues 240 or 250 are desirable fragments. Still other fragments CT-CRMs of this invention may be selected. Additional fragments of the CT-CRM holotoxin may contain less then five repetitions of the CT-B subunits or truncated CT-B subunits. The foregoing fragments may also contain one or more of the specific mutations described above.
Other suitable CT-CRM proteins may include those in which one or more of the amino acid residues includes a substituted group. Still another suitable CT-CRM holotoxin protein is one in which the CT-CRM polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol). Another suitable CT-CRM protein is one in which additional amino acids are fused to the polypeptide, such as a leader or secretory sequence, or a sequence which is employed to enhance the immunogenicity of the CT-CRM protein. Still other modifications of the CT-CRMs include the above-mentioned deletion of the CT-A signal or leader sequence at the N terminus of CT, i.e., amino acids 1-18 of SEQ ID NO: 1 and/or the deletion of the CT-B signal or leader sequence at amino acids 259-279 of SEQ ID NO: 1, and/or the deletion of other regions that do not effect immunogenicity. Similarly, a modification of the CT-CRMs described herein includes include replacing either signal or leader sequence with another signal or leader sequence. See, e.g., U.S. Pat. No. 5,780,601, incorporated by reference herein.
Still another example of suitable CT-CRM proteins are those in which optional amino acids (e.g., -Gly-Ser-) or other amino acid or chemical compound spacers may be included at the termini of the polypeptide for the purpose of linking multiple holotoxin proteins together or to a carrier. For example, useful CT-CRMs may include one or more of the above-described CT-CRMs coupled to a carrier protein. Alternatively, a useful CT-CRM may be present in a fusion protein containing multiple CT-CRMs, optionally coupled to carrier protein.
For these embodiments, the carrier protein is desirably a protein or other molecule that can enhance the immunogenicity of the selected CT-CRM. Such a carrier may be a larger molecule that also has an adjuvanting effect. Exemplary conventional protein carriers include, without limitation, E. coli DnaK protein, galactokinase (GalK, which catalyzes the first step of galactose metabolism in bacteria), ubiquitin, α-mating factor, β-galactosidase, and influenza NS-1 protein. Toxoids (i.e., the sequence which encodes the naturally occurring toxin, with sufficient modifications to eliminate its toxic activity) such as diphtheria toxoid and tetanus toxoid, their respective toxins, and any mutant forms of these proteins, such as CRM197 (a non-toxic form of diphtheria toxin, see U.S. Pat. No. 5,614,382), may also be employed as carriers. Other carriers include exotoxin A of Pseudomonas aeruginosa, heat labile toxins of E. coli and rotaviral particles (including rotavirus and VP6 particles). Alternatively, a fragment or epitope of the carrier protein or other immunogenic protein may be used. For example, a hapten may be coupled to a T cell epitope of a bacterial toxin. See U.S. Pat. No. 5,785,973. Similarly a variety of bacterial heat shock proteins, e.g., mycobacterial hsp-70 may be used. Glutathione-S-transferase (GST) is another useful carrier. One of skill in the art can readily select an appropriate carrier for use in this context. The fusion proteins may be formed by standard techniques for coupling proteinaceous materials. Fusions may be expressed from fused gene constructs prepared by recombinant DNA techniques as described below.
Other suitable CT-CRMs described herein can differ from the specifically exemplified CT-CRMs by modifications that do not revive enzymatic toxicity, and do not diminish adjuventicity, or by combinations of such attributes. For example, conservative amino acid changes may be made, which, although they alter the primary sequence of the CT-CRM protein, do not normally alter its function. In making such changes, the hydropathic index of amino acids can be considered. The importance of the hydropathic amino acid index in conferring interactive biologic function on a polipeptide is generally understood in the art byte & Doolittle, 1982 J. Mol. Biol., 157(1):105-132). It is known that certain amino acids can be substituted for other amino acids having a similar hydropathic index or score and still result in a polypeptide with similar biological activity. Each amino acid has been assigned a hydropathic index on the basis of its hydrophobicity and charge characteristics. Those indices are: isoleucine (+4.5); valine (+4.2); leucine (+3.8); phenylalanine (+2.8); cysteine/cystine (+2.5); methionine (+1.9); alanine (+1.8); glycine (−0.4); threonine (−0.7); serine (−0.8); tryptophan (−0.9); tyrosine (−1.3); proline (−1.6); histidine (−3.2); glutamate (−3.5); glutamine (−3.5); aspartate (−3.5); asparagine (−3.5); lysine (−3.9); and arginine (−4.5).
It is believed that the relative hydropathic character of the amino acid residue determines the secondary and tertiary structure of the resultant polypeptide, which in turn defines the interaction of the polypeptide with other molecules, such as enzymes, substrates, receptors, antibodies, antigens, and the like. It is known in the art that an amino acid can be substituted by another amino acid having a similar hydropathic index and still obtain a fictionally equivalent polypeptide. In such changes, the substitution of amino acids whose hydropathic indices are within +/−2 is preferred, those within +/−1 are particularly preferred, and those within +/−0.5 are even more particularly preferred.
Substitution of like amino acids can also be made on the basis of hydrophilicity, particularly where the biologically functional equivalent polypeptide or peptide thereby created is intended for use in immunological embodiments. U.S. Pat. No. 4,554,101, incorporated herein by reference, states that the greatest local average hydrophilicity of a polypeptide, as governed by the hydrophilicity of its adjacent amino acids, correlates with its immunogenicity and antigenicity, i.e. with a biological property of the polypeptide.
As detailed in U.S. Pat. No. 4,554,101, the following hydrophilicity values have been assigned to amino acid residues: arginine (+3.0); lysine (+3.0); aspartate (+3.0±1); glutamate (+3.0±1); serine (+0.3); asparagine (+0.2); glutamine (+0.2); glycine (0); proline (−0.5±1); threonine (−0.4); alanine (−0.5); histidine (−0.5); cysteine (−1.0); methionine (−1.3); valine (−1.5); leucine (−1.8); isoleucine (−1.8); tyrosine (−2.3); phenylalanine (−2.5); tryptophan (−3.4). It is understood that an amino acid can be substituted for another having a similar hydrophilicity value and still obtain a biologically equivalent, and in particular, an immunologically equivalent polypeptide. In such changes, the substitution of amino acids whose hydrophilicity values are within ±2 is preferred; those within ±1 are particularly preferred; and those within ±0.5 are even more particularly preferred.
As outlined above, amino acid substitutions are generally based on the relative similarity of the amino acid side-chain substituents, for example, their hydrophobicity, hydrophilicity, charge, size, and the like. Exemplary substitutions which take various of the foregoing characteristics into consideration are well known to those of skill in the art and include: arginine and lysine; glutamate and aspartate; serine and threonine; glutamine and asparagine; and valine,-leucine and isoleucine.
In addition, modifications, which do not normally alter the primary sequence of the CT-CRM protein, include in vivo or in vitro chemical derivatization of polypeptides, e.g., acetylation, methylation, or carboxylation. Also included as CT-CRMs of this invention are these proteins modified by glycosylation, e.g., those made by modifying the glycosylation patterns of a polypeptide during its synthesis and processing or in further processing steps; or by exposing the polypeptide to enzymes which affect glycosylation, such as mammalian glycosylating or deglycosylating enzymes. Also embraced as CT-CRMs are the above-identified mutagenized sequences, which have phosphorylated amino acid residues, e.g., phosphotyrosine, phosphoserine, or phosphothreonine.
Also included as CT-CRW of this invention are the above sequences that have been modified using ordinary molecular biological techniques so as to improve their resistance to proteolytic degradation or to optimize solubility properties. Among such CT-CRMs are included those containing residues other than naturally occurring L-amino acids, e.g., D-amino acids or non-naturally occurring synthetic amino acids. Among other known modifications which may be present in CT-CRMs of the present invention are, without limitation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cystine, formation of pyroglutarrate, formulation, gamma-carboxylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination.
The mutant CT-CRMs of this invention are thus holotoxins and exhibit reduced toxicitiy or are substantially less toxic than wild-type CT. As used herein, the terms and phrases “the holotoxin has reduced toxicity” or “substantially less toxic” or the like mean that the CT-CRM mutant of this invention, such as the four CT-CRM mutants described herein (CT-CRMI16A, CT-CRMV72Y, CT-CRMI16A, S68Y, and CT-CRMS68Y, V72Y), exhibits a substantially lower toxicity per unit of purified toxin protein compared to the wild-type CT. This “reduced toxicity” enables each mutant to be used as an adjuvant in an immunogenic composition without causing significant side effects, particularly those known to be associated with CT, e.g., diarrhea. As described in more detail below, the mutant CT-CRC of this invention display significantly lower levels of toxicity than the wild-type CT in the Y-1 mouse adrenal cell assay, and a significantly reduced ADP-ribosyltransferase activity when compared to wild-type CT.
The immunogenic mutant CT-CRMs according to the present invention exhibit a balance of reduced toxicity and retained adjuvanticity, such that the resulting mutant CT protein functions as an adjuvant while being tolerated safely by the vertebrate host to which it is introduced. As indicated in the examples below, results in murine model assay systems indicate that the mutant CT-CRMs disclosed herein were able to significantly augment mucosal and systemic immune responses following intranasal administration of disparate antigens. Furthermore, even in the presence of pre-existing anti-CT immune responses, the mutant CT-CRMs were able to serve as efficient mucosal adjuvants. The studies which support these characteristics of the CT-CRMs of this invention are summarized below and more specifically stated in the Examples.
To evaluate the efficacy of the mutant CT-CRMs as mucosal adjuvants for compositions containing bacterial or viral antigens that have been identified as candidates for inclusion in immunogenic compositions, three disparate model antigen systems were examined: (1) the recombinant P4 outer membrane protein (also known as protein “e” (rP4)) of the nontypable Haemophilus influenzae bacterium (NTHi), (see U.S. Pat. No. 5,601,831), (2) the native UspA2 outer membrane protein of the Moraxella catarrhalis bacterium (International Patent Publication No. WO 98/28333), and (3) the native fusion glycoprotein (F protein) of respiratory syncytial virus (RSV) (see U.S. Pat. No. 5,223,254). The mutant CT-CRMs were compared with each other, and to CT-CRME29H and the wild-type CT as an adjuvant for the NTHi rP4. In a first study, the adjuvanting ability of the mutant CT-CRMI16A to enhance the induction of systemic and mucosal antibodies to rP4 were assessed and compared with that of wild-type CT and CT-CRME29H. The results indicated that the CT-CRMI16A, like the wild-type CT and CT-CRME29H, augmented the capacity of rP4 protein to elicit systemic and humoral immune responses (see Tables 8 and 9). For example, six weeks after tertiary IN immunization, the anti-rP4 IgG antibody titers of mice immunized with rP4 protein formulated with either CT-CRMI16A or CT-CRME29H were 40 times greater than that of mice immunized with the recombinant proteins in PBS alone. The antibody titers (IgG) of mice administered the recombinant protein plus wild-type CT holotoxin at a concentration of 1 μg were elevated 67-fold in comparison to antibody titers in mice administered recombinant rP4 alone in saline six weeks after the primary IN immunization. The antibody titers of mice immunized with 1 μg of the mutant, CT-CRME29H were elevated 48-fold over antibody titers in mice immunized with rP4 alone. In comparison, the antibody titers of mice immunized with 1 μg and 0.1 μg of the mutant, CT-CRMI16A, were increased 15-fold and 27-fold respectively over the anti-rP4 antibody titers in mice immunized with rP4 alone in saline.
An examination of the protein-specific antibodies in the mucosal secretions two weeks after tertiary immunization further indicated that the CT-CRMI16A facilitated the generation of local immune responses against the rP4 protein Moreover, the anti-rP4 antibody titers were comparable to those induced by wild-type CT adjuvanted immunogenic composition (Table 9).
To test and compare the adjuvanting effects of mutant CT-CPMs in formulations containing 1 μg recombinant rP4 and 1 μg of one of the mutant CT-CRMs (CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y, and CT-CRMS68Y,V72Y) with a formulation containing 1 μg rP4 and 1 μg CT-CRME29H, or 1 μg rP4 alone in saline. The various compositions were delivered intranasally to female BALB/c mice, and the anti-rP4 IgG and IgA titers measured at weeks 3 and 5, and at week 5, day 6. The data suggest that CT-CRMs, CT-CRMI16A and CT-CRMV72Y are as potent as CT-CRME29H in inducing systemic as well as mucosal anti-rP4 antibody response (Tables 10 and 11). The serum IgG titers of anti-rP4 antibody induced by the formulation containing rP4 and CT-CRMI16A at week 5, day 6 was 22-fold greater than that induced by rP4 alone and half of the IgG levels induced by CT-CRME29H. However, serum IgG titers of anti-rP4 antibody induced by the formulation containing rP4 and CT-CRMV72Y was 1.2-fold more than that induced by CT-CRME29H and 53-fold greater than that induced by rP4 alone. Although the rP4-specific IgG titers induced by CT-CRMI16A,S68Y and CT-CRMS68Y,V72Y were only approximately one-fifth of that induced by CT-CRMI16A and CT-CRMV72Y, these levels were still significantly higher than that induced by rP4 alone in saline.
The protein-specific IgA antibody titers in the sera of mice immunized with CT-CRMs, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y and CT-CRMS68Y,V72Y were 6 to 23-fold greater than those of mice immunized IN with the rP4 alone.
The protein-specific IgA antibody titers in the bronchoalveolar wash, nasal wash, saliva and vaginal wash of mice immunized with CT-CRMs, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y and CT-CRMS68Y,V72Y were comparable to the IgA levels in the mucosal wash pools of mice immunized with CT-CRME29H, but significantly greater than those of mice immunized with rP4 alone. (See Table 11).
In the above study, anti-rP4 antibody titers in the serum of each individual mouse in the six groups were also assessed. Specifically, 41 days following IN administration, IgA and IgG including IgG subclass IgGI, IgG2a, IgG2b and IgG3 endpoint titers were determined by ELISA The results indicate that IgA and IgG subclass titers in each individual mouse receiving the formulation containing rP4 and any one of the four nutant CT-CRMs were significantly higher than the IgA and IgG titers in animals receiving only the rP4 antigen in saline. (See Tables 12-17). The results further indicate that the IgA and IgG titers in animals receiving rP4 and one of the mutant CT-CRMs, CT-CRMI16A, CT-CRMI16A,S68Y, and CT-CRMV72Y, were comparable to the IgA and IgG titers detected in mice receiving rP4 plus CT-CRME29H.
The capacity of the CT-CRMs of the present invention to augment systemic and mucosal immune responses against respiratory syncytial virus (RSV) glycoproteins was examined using the purified native fusion (F) protein Previously, it was demonstrated that BALB/c mice immunized IN with F protein adjuvanted with either CT or CT-CRME29H generated systemic and local IgG and IgA titers (Tebbey et al, cited above). This study also indicated that pre-existing anti-CT antibodies did not have a negative impact on the level of local or systemic anti-F protein IgA and IgG antibodies. Indeed, the study indicated that pre-existing anti-CT antibodies were beneficial for the generation of an augmented anti-F protein antibody response. Additionally, the data also suggested a mechanism involving the neutralization of infectious virus by either mucosal or humoral immunoglobulins that are stimulated in response to the IN immunization protocol containing F/CT-CRME29H. In the present study, purified F protein (3 μg/dose) alone in saline or in a formulation containing 0.1 or 1 μg of the wild-type CT or 0.1 or 1 μg of one of the mutant CT-CRMs (CT-CRME29H, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y and CT-CRMS68Y,V72Y) was administered IN to BALB/c mice. The protein-specific IgG and IgA antibody titers in the bronchoalveolar wash, nasal wash and vaginal wash of mice were determined. The protein-specific IgG and IgA antibody titers in the bronchoalveolar wash, nasal wash and vaginal wash of mice immunized with 1 μg of CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y or CT-CRMS68Y,V72Y were comparable to the IgG and IgA levels in the mucosal wash pools of mice immunized with wild-type CT or CT-CRME29H (See Table 19). The mucosal protein-specific IgG levels in mice immunized with 0.1 μg of CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y or CT-CRMS68Y,V72Y though significantly higher than the levels detected in mice immunized with F-protein in saline, were nevertheless one-third to one-tenth less than the levels detected in mice immunized with 1 μg of the CT-CRMs. In contrast, significantly elevated levels of IgA in mucosal washes were only observed in mice immunized with 1 μg of the mutant CT-CRMs.
The capacity of mutant CT-CRMs to augment systemic and mucosal immune responses in mice against the native UspA2 outer membrane protein of M. catarrhalis was examined. Purified UspA2 (5 μg/dose) alone in 10 μl saline or in a 10 μl formulation containing 0.1 μg/dose of a mutant CT-CRM (CT-CRME29H, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y or CT-CRMS68Y,V72Y) was administered IN at days 0, 7 and 14. Protein-specific IgG and IgA levels in the serum and in mucosal lavages were examined at day 28. Statistically significant levels of IgG and IgA were detected only in the serum of animals immunized with CT-CRME29H, CT-CRMI16A,S68Y and CT-CRMV72Y. Significant levels of IgG were also detected in the bronchial wash of animals immunized with CT-CRME29H, CT-CRMI16A,S68Y and CT-CRMV72.
B. Nucleic Acid Molecules Encoding CT-CRMs
Another aspect of this invention includes isolated, synthetic or recombinant nucleic acid molecules and sequences encoding the above-described CT-CRMs having the specified site directed mutations or fragments that may further contain one or more of those mutations.
An isolated nucleotide molecule comprising a nucleic acid sequence encoding a CT-CRM protein may be preferably under the control of regulatory sequences that direct expression of the CT-CRM in a host cell. As described herein, such nucleic acid molecules may be used to express the CT-CRM protein in vitro or to permit expression of the CT-CRM protein in vivo in a human.
As used herein, the term “isolated nucleotide molecule or sequence” refers to a nucleic acid segment or fragment which is free from contamination with other biological components that may be associated with the molecule or sequence in its natural environment. For example, one embodiment of an isolated nucleotide molecule or sequence of this invention is a sequence separated from sequences which flank it in a naturally occurring state, e.g., a DNA fragment which has been removed from the sequences which are normally adjacent to the fragment, such as the sequences adjacent to the fragment in a genome in which it naturally occurs. Further, the nucleotide sequences and molecules of this invention have been altered to encode a CT-CRM protein of this invention. Thus, the term “isolated nucleic acid molecule or sequence” also applies to nucleic acid sequences or molecules that have been substantially purified from other components that naturally accompany the unmutagenized nucleic acid, e.g., RNA or DNA or proteins, in the cell An isolated nucleotide molecule or sequence of this invention also encompasses sequence and molecules that have been prepared by other conventional methods, such as recombinant methods, synthetic methods, e.g., mutagenesis, or combinations of such methods. The nucleotide sequences or molecules of this invention should not be construed as being limited solely to the specific nucleotide sequences presented herein, but rather should be construed to include any and all nucleotide sequences which share homology (i.e., have sequence identity) with the nucleotide sequences presented herein.
The terms “substantial homology” or “substantial similarity,” when referring to a nucleic acid or fragment thereof indicates that, when optimally aligned with appropriate nucleotide insertions or deletions with another nucleic acid (or its complementary strand), there is nucleotide sequence identity in at least about 70% of the nucleotide bases, as measured by any well-known algorithm of sequence identity, such as FASTA, a program in GCG Version 6.1. The term “homologous” as used herein, refers to the sequence similarity between two polymeric molecules, e.g., between two nucleic acid molecules, e.g., two DNA molecules or two RNA molecules, or between two polypeptide molecules. When a nucleotide or amino acid position in both of the two molecules is occupied by the same monomeric nucleotide or amino acid, e.g., if a position in each of two DNA molecules is occupied by adenine, then they are homologous at that position. The homology between two sequences is a direct function of the number of matching or homologous positions, e.g., if half (e.g., five positions in a polymer ten subunits in length) of the positions in two compound sequences are homologous then the two sequences are 50% homologous. If 90% of the positions, e.g., 9 of 10, are matched or homologous, the two sequences share 90% homology. By way of example, the DNA sequences 3′ATTGCC5′ and 3TATGCG5′ share 50% homology. By the term “substantially homologous” as used herein, is meant DNA or RNA which is about 70% homologous, more preferably about 80% homologous and most preferably about 90% homologous to the desired nucleic acid.
The invention is also directed to an isolated nucleotide molecule comprising a nucleic acid sequence that is at least 70%, 80% or 90% homologous to a nucleic acid sequence encoding a CT-CRM protein of this invention that has reduced enzymatic toxicity compared to wild-type CT protein and that retains adjuvanticity of the wild-type CT. Furthermore, due to the degeneracy of the genetic code, any three-nucleotide codon that encodes a mutated amino acid residue of CT-CRM, described herein is within the scope of the invention.
Where, as discussed herein, CT-CRMs, nutant CT-A subunits or mutant CT-B subunits, and/or DNA sequences encoding them, or other sequences useful in nucleic acid molecules or compositions described herein are defined by their percent homologies or identities to identified sequences, the algorithms used to calculate the percent homologies or percent identities include the following: the Smith-Waterman algorithm (J. F. Collins et al, 1988, Comput. Appl. Biosci., 4:67-72; J. F. Collins et al, Molecular Sequence Comparison and Alignment, (M. J. Bishop et al, eds.) In Practical Approach Series: Nucleic Acid and Protein Sequence Analysis XVIII, IRL Press: Oxford, England, UK (1987) pp. 417), and the BLAST and FASTA programs (E. G. Shpaer et al, 1996, Genomics, 38:179-191). These references are incorporated herein by reference.
By describing two DNAs as being “operably linked” as used herein, is meant that a single-stranded or double-stranded DNA comprises each of the two DNAs and that the two DNAs are arranged within the DNA in such a manner that at least one of the DNA sequences is able to exert a physiological effect by which it is characterized upon the other.
Preferably, for use in producing a CT-CRM protein of this invention or administering it for in vivo production in a cell, each CT-CRM protein encoding sequence and necessary regulatory sequences are present in a separate viral or non-viral recombinant vector (including non-viral methods of delivery of a nucleic acid molecule into a cell). Alternatively, two or more of these nucleic acid sequences encoding duplicate copies of a CT-CRM protein or encoding multiple different CT-CRMs of this invention may be contained in a polycistronic transcript, i.e., a single molecule designed to express multiple gene products.
The invention further relates to vectors, particularly plasmids, containing isolated and purified DNA sequences comprising DNA sequences that encode an immunogenic mutant cholera holotoxin. Desirable embodiments include plasmids containing DNA sequences encoding CT-CRMs having a single amino acid substitution at amino acid position 16 or 72 or double amino acid substitution at amino acid position 16 and 68 or 68 and 72 respectively. By the term “vector” as used herein, is meant a DNA molecule derived from viral or non-viral, e.g., bacterial, species that has been designed to encode an exogenous or heterologous nucleic acid sequence. Thus, the term includes conventional bacterial plasmids. Such plasmids or vectors can include plasmid sequences from viruses or phages. Such vectors include chromosomal, episomal and virus-derived vectors, e.g., vectors derived from bacterial plasmids, bacteriophages, yeast episomes, yeast chromosomal elements, and viruses. Vectors may also be derived from combinations thereof such as those derived from plasmid and bacteriophage genetic elements, cosmids, and phagemids. The term also includes non-replicating viruses that transfer a gene from one cell to another. The term should also be construed to include non-plasmid and non-viral compounds which facilitate transfer of nucleic acid into cells, such as, for example, polylysine compounds and the like.
The nucleic acid molecules of the invention include non-viral vectors or methods for delivery of the sequence encoding the CT-CRM protein to a host cell according to this invention A variety of non-viral vectors are known in the art and may include, without limitation, plasmids, bacterial vectors, bacteriophage vectors, “naked” DNA and DNA condensed with cationic lipids or polymers.
Examples of bacterial vectors include, but are not limited to, sequences derived from bacille Calmette Guérin (BCG), Salmonella, Shigella, E. coli, and Listeria, among others. Suitable plasmid vectors include, for example, pBR322, pBR325, pACYC177, pACYC184, pUC8, pUC9, pUC18, pUC19, pLG339, pR290, pK37, pKC101, pAC105, pVA51, pKH47, pUB 10, pMB9, pBR325, ColE1, pSC101, pBR313, pML21, RSF2124, pCR1, RP4, pBAD18, and pBR328.
Examples of suitable inducible Escherichia coli expression vectors include pTrc (Amann et al., 1988 Gene, 69:301-315), the arabinose expression vectors (e.g., pBAD18, Guzman et al, 1995 J. Bacteriol., 177:4121-4130), and pETIId (Studier et al., 1990 Methods in Enzymology, 185:60-89). Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pETIId vector relies on transcription from a T7 gn10-lac fusion promoter mediated by a coexpressed viral RNA polymerase T7 gn1. This viral polymerase is supplied by host strains BL21 (DE3) or HMS I 74(DE3) from a resident prophage harboring a T7 gn1 gene under the transcriptional control of the lacUV5 promoter. The pBAD system relies on the inducible arabinose promoter that is regulated by the araC gene. The promoter is induced in the presence of arabinose.
As one example, a plasmid, designated pLP903, contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic mutant CT-CRM with substantially reduced toxicity wherein the amino acid alanine is substituted for isoleucine at amino acid position 16 in the A subunit. A second plasmid, designated pLP905, contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic mutant CT-CRM with substantially reduced toxicity wherein the amino acid tyrosine is substituted for valine at the amino acid position 72 in the A subunit. Another exemplary plasmid is designated pLP904. This plasmid contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic, mutant CT-CRM with substantially reduced toxicity wherein the amino acid alanine is substituted for isoleucine at amino acid position 16, and amino acid tyrosine is substituted for serine at amino acid position 68 in the A subunit. Another plasmid exemplified in this invention is designated pLP906. It contains an isolated and purified DNA sequence comprising a DNA sequence encoding an immunogenic, mutant CT-CRM with substantially reduced toxicity wherein the amino acid tyrosine is substituted for serine at the amino acid position 68, and amino acid tyrosine is substituted for valine at amino acid position 72 in the A subunit.
Another type of useful vector is a single or double-stranded bacteriophage vector. For example, a suitable cloning vector includes, but is not limited to the vectors such as bacteriophage λ vector system, λgt11, μgt μWES.tB, Charon 4, λgt-WES-λB, Charon 28, Charon 4A, λgt-1-λBC, λgt-1-λB, M13mp7, M13mp8, or M13mp9, among others.
In another embodiment, the expression vector is a yeast expression vector. Examples of vectors for expression in a yeast such as S. cerevisiae include pYepSec I (Baldari, et al., 1987 Protein Eng., 1(5):433-437), pMFa (Kujan and Herskowitz, 1982 Cell, 30(3):933-943), pJRY88 (Schultz et al., 1987 Gene, 61(2): 123-133), and pYES2 (Invitrogen Corporation, San Diego, Calif.).
Alternatively, baculovirus expression vectors are used. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf9 or Sf21 cells) include the pAc series (Smith et al., 1983 Biotechnol., 24:434-443) and the pVL series (Luckow and Summers, 1989 Virol., 170(1):31-39). In yet another embodiment, a mammalian expression vector is used for expression in mammalian cells. Examples of mammalian expression vectors include pCDM8 (Seed, 1987 Nature, 329:840-842) and pMT2PC (Kaufman et al., 1987 EMBO J., 6(1):187-93). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.
One type of recombinant vector is a recombinant single or double-stranded RNA or DNA viral vector. A variety of viral vector systems are known in the art. Examples of such vectors include, without limitation, recombinant adenoviral vectors, herpes simplex virus (HSV)-based vectors, adeno-associated viral (AAV) vectors, hybrid adenoviral/AAV vectors, recombinant retroviruses or lentiviruses, recombinant poxvirus vectors, recombinant vaccinia virus vectors, SV-40 vectors, insect viruses such as baculoviruses, and the like that are constructed to carry or express a selected nucleic acid composition of interest.
Retrovirus vectors that can be employed include those described in EP 0 415 731; International Patent Publication Nos. WO 90/07936; WO 94/03622; WO 93/25698; and WO 93/25234; U.S. Pat. No. 5,219,740; International Patent Publication Nos. WO 93/11230 and WO 93/10218; Vile and Hart, 1993 Cancer Res. 53:3860-3864; Vile and Hart, 1993 Cancer Res. 53:962-967; Ram et al., 1993 Cancer Res. 53:83-88; Takamiya et al., 1992 J. Neurosci. Res. 33:493-503; Baba et al., 1993 J. Neurosurg. 79:729-735; U.S. Pat. No. 4,777,127; GB Patent No. 2,200,651; and EP 0 345 242. Examples of suitable recombinant retroviruses include those described in International Patent Publication No. WO 91/02805.
Alphavirus-based vectors may also be used as the nucleic acid molecule encoding the CT-CRM protein. Such vectors can be constructed from a wide variety of viruses, including, for example, Sindbis virus vectors, Semliki forest virus (ATCC VR-67; ATCC VR-1247), Ross River virus (ATCC VR-373; ATCC VR-1246) and Venezuelan equine encephalitis virus (ATCC VR-923; ATCC VR-1250; ATCC VR 1249; ATCC VR-532). Representative examples of such vector systems include those described in U.S. Pat. Nos. 5,091,309; 5,217,879; and 5,185,440; and International Patent Publication Nos. WO 92/10578; WO 94/21792; WO 95/27069; WO 95/27044; and WO 95/07994.
Examples of adenoviral vectors include those described by Berkner, 1988 Biotechniques 6:616-627; Rosenfeld et al., 1991 Science 252:431-434; International Patent Publication No. WO 93/19191; Kolls et al., 1994 PNAS 91:215-219; Kass-Eisler et al., 1993 PNAS 90:11498-11502; Guzman et al., 1993 Circulation 88:2838-2848; Guzman et al., 1993 Cir. Res. 73:1202-1207; Zabner et al., 1993 Cell 75:207-216; Li et al., 1993 Hum. Gene Ther. 4:403-409; Cailaud et al., 1993 Eur. J. Neurosci. 5:1287-1291; Vincent et al., 1993 Nat. Genet. 5:130-134; Jaffe et al., 1992 Nat. Genet. 1:372-378; and Levrero et al., 1991 Gene 101:195-202. Exemplary adenoviral vectors include those described in International Patent Publication Nos. WO 94/12649; WO 93/03769; WO 93/19191; WO 94/28938; WO 95/11984 and WO 95/00655. Other adenoviral vectors include those derived from chimpanzee adenoviruses, such as those described in U.S. Pat. No. 6,083,716.
Another viral vector is based on a parvovirus such as an adeno-associated virus (AAV). Representative examples include the AAV vectors described in International Patent Publication No. WO 93/09239, Samulski et al., 1989 J. Virol. 63:3822-3828; Mendelson et al., 1988 Virol. 166:154-165; and Flotte et al., 1993 PNAS 90:10613-10617. Other particularly desirable AAV vectors include those based upon AAV1; see, International Patent Publication No. WO 00/28061, published May 18, 2000. Other desirable AAV vectors include those which are pseudotyped, i.e., contain a minigene composed of AAV 5′ITRS, a transgene, and AAV 3′ITRs packaged in a capsid of an AAV serotype heterologous to the AAV ITRs. Methods of producing such pseudotyped AAV vectors are described in detail in International Patent Publication No. WO01/83692.
In an embodiment in which the nucleic acid molecule of the invention is “naked DNA”, it may be combined with polymers including traditional polymers and non-traditional polymers such as cyclodextrin-containing polymers and protective, interactive noncondensing polymers, among others. The “naked” DNA and DNA condensed with cationic lipids or polymers are typically delivered to the cells using chemical methods. A number of chemical methods are known in the art for cell delivery and include using lipids, polymers, or proteins to complex with DNA, optionally condensing the same into particles, and delivering to the cells. Another non-viral chemical method includes using cations to condense DNA, which is then placed in a liposome and used according to the present invention. See, C. Henry, 2001 Chemical and Engineering News, 79(48):35-41.
The nucleic acid molecule encoding the CT-CRM of this invention is introduced directly into the cells either as “naked” DNA (U.S. Pat. No. 5,580,859) or formulated in compositions with agents, which facilitate immunization, such as bupivicaine and other local anesthetics (U.S. Pat. No. 6,127,170).
All components of the viral and non-viral vectors above may be readily selected from among known materials in the art and available from the pharmaceutical industry. Selection of the vector components and regulatory sequences are not considered a limitation on this invention. Each nucleic acid sequence encoding a CT-CRM protein according to this invention is preferably under the control of regulatory sequences that direct the replication and generation of the product of each nucleic acid sequence in a mammalian or vertebrate cell. By the term “promoter/regulatory sequence” is meant a DNA sequence required for expression of a nucleic acid operably liked to the promoter/regulatory sequence. In some instances, the promoter/regulatory sequence may function in a tissue specific manner. For example, the promoter/regulatory sequence is only capable of driving expression in a cell of a particular tissue type. In some instances, this sequence may be the core promoter sequence and in other instances, this sequence may also include an enhancer sequence and other regulatory elements that are required for expression in a tissue-specific manner.
Preferably, the nucleic acid molecule encoding a CT-CRM protein of this invention and/or the recombinant vector further comprises regulatory sequences. For example, such regulatory sequences comprise a promoter that drives expression of the CT-CRM protein. Preferably the promoter/regulatory sequence is positioned at the 5′ end of the coding sequence such that it drives expression of the CT-CRM protein in a cell.
Suitable promoters may be readily selected from among constitutive promoters, inducible promoters, tissue-specific promoters and others. Examples of constitutive promoters that are non-specific in activity and employed in the nucleic acid molecules encoding the CT-CRM protein of this invention include, without limitation, the retroviral Rous sarcoma virus (RSV) promoter, the retroviral LTR promoter (optionally with the RSV enhancer), the cytomegalovirus (CMV) promoter (optionally with the CMV enhancer) (see, e.g., Boshart et al, Cell, 41:521-530 (1985)), the SV40 promoter, the dihydrofolate reductase promoter, the β-actin promoter, the phosphoglycerol kinase (PGK) promoter, and the EF1α promoter (Invitrogen).
Inducible promoters that are regulated by exogenously supplied compounds, include, without limitation, the arabinose promoter, the zinc-inducible sheep metallothionine (MT) promoter, the dexamethasone (Dex)-inducible mouse mammary tumor virus (MMTV) promoter, the T7 polymerase promoter system (WO 98/10088); the ecdysone insect promoter (No et al, 1996 Proc. Natl. Acad. Sci. USA, 93:3346-3351), the tetracycline-repressible system (Gossen et al, 1992 Proc. Natl. Acad. Sci. USA, 89:5547-5551), the tetracycline-inducible system (Gossen et al, 1995 Science, 268:1766-1769, see also Harvey et al, 1998 Curr. Opin. Chem. Biol., 2:512-518), the RU486-inducible system (Wang et al, 1997 Nat. Biotech., 15:239-243 and Wang et al, 1997 Gene Ther., 4:432-441) and the rapamycin-inducible system (Magari et al, 1997 J. Clin. Invest., 100: 2865-2872). A particularly preferred promoter for use in expression systems for CT-CRs is an arabinose inducible promoter.
Other types of inducible promoters that may be useful in this context are those regulated by a specific physiological state, e.g., temperature or acute phase or in replicating cells only. Useful tissue-specific promoters include the promoters from genes encoding skeletal β-actin, myosin light chain 2A, dystrophin, muscle creatine kinase, as well as synthetic muscle promoters with activities higher than naturally-occurring promoters (see Li et al., 1999 Nat. Biotech., 17:241-245). Examples of promoters that are tissue-specific are known for liver (albumin, Miyatake et al. 1997 J. Virol., 71:5124-32; hepatitis B virus core promoter, Sandig et al., 1996 Gene Ther., 3: 1002-9; alpha-fetoprotein (AFP), Arbuthnot et al., 1996 Hum. Gene Ther., 7:1503-14), bone (osteocalcin, Stein et al., 1997 Mol. Biol. Rep., 24:185-96; bone sialoprotein, Chen et al., 1996 J. Bone Miner. Res., 11:654-64), lymphocytes (CD2, Hansal et al., 1988 J. Immunol., 161:1063-8; immunoglobulin heavy chain; T cell receptor a chain), neuronal (neuron-specific enolase (NSE) promoter, Andersen et al. 1993 Cell. Mol. Neurobiol., 13:503-15; neurofilament light-chain gene, Piccioli et al., 1991 Proc. Natl. Acad. Sci. USA, 88:5611-5; the neuron-specific B ngf gene, Piccioli et al., 1995 Neuron, 15:373-84); among others. See, e.g., International Patent Publication No. WO00/55335 for additional lists of known promoters useful in this context.
Additional regulatory sequences for inclusion in a nucleic acid sequence, molecule or vector of this invention include, without limitation, an enhancer sequence, a polyadenylation sequence, a splice donor sequence and a splice acceptor sequence, a site for transcription initiation and termination positioned at the beginning and end, respectively, of the polypeptide to be translated, a ribosome binding site for translation in the transcribed region, an epitope tag, a nuclear localization sequence, an IRES element, a Goldberg-Hogness “TATA” element, a restriction enzyme cleavage site, a selectable marker and the like. Enhancer sequences include, e.g., the 72 bp tandem repeat of SV40 DNA or the retroviral long terminal repeats or LTRs, etc. and are employed to increase transcriptional efficiency. Selection of promoters and other common vector elements are conventional and many such sequences are available with which to design the nucleotide molecules and vectors useful in this invention See, e.g., Sambrook et al, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, New York, (1989) and references cited therein at, for example, pages 3.18-3.26 and 16.17-16.27 and Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, New York (1989). One of skill in the art may readily select from among such known regulatory sequences to prepare molecules of this invention The selection of such regulatory sequences is not a limitation of this invention.
C. Methods for Making the CT-CRM Proteins and Nucleotide Molecules of this Invention
In view of the demonstrated utility of mutant CT-CRMs as adjuvants for antigenic compositions, production of suitable quantities of mutant CT-CRMs is desirable. The preparation or synthesis of the nucleotide sequences and CT-CRMs, as well as compositions containing the nucleotide molecules or CT-CRM protein of this invention disclosed herein is well within the ability of the person having ordinary skill in the art using available material. The synthesis methods are not a limitation of this invention The examples below detail presently preferred embodiments of synthesis of sequences encoding the CT-CRMs of this invention.
The CT-CRMs and nucleotide molecules and sequences of this invention may be produced by chemical synthesis methods, recombinant genetic engineering methods, site directed mutagenesis, among others, and combinations of such methods. For example, the nucleotide sequences/CT-CRMs of the invention may be prepared conventionally by resort to known chemical synthesis techniques, e.g., solid-phase chemical synthesis, such as described by Merrifield, 1963 J. Amer. Chem. Soc., 85:2149-2154; J. Stuart and J. Young, Solid Phase Peptide Synthesis, Pierce Chemical Company, Rockford, Ill. (1984); Matteucci et al., 1981 J. Am. Chem. Soc., 103:3185; Alvarado-Urbina et al., 1980 Science, 214:270; and Sinha, N. D. et al., 1984 Nucl. Acids Res., 13:4539, among others. See, also, e.g., PROTEINS—STRUCTURE AND MOLECULAR PROPERTIES, 2nd Ed., T. E. Creighton, W.H. Freeman and Company, New York, 1993; Wold, F., “Posttranslational Protein Modifications: Perspectives and Prospects”, pgs. 1-12 in POSTTRANSLATIONAL COVALENT MODIFICATION OF PROTEINS, B. C. Johnson, Ed., Academic Press, New York, 1983; Seifter et al., 1990 Meth. Enzymol., 182:626-646, and Rattan et al., 1992 Ann. N.Y. Acad. Sci., 663:48-62.
Alternatively, compositions of this invention may be constructed recombinantly using conventional molecular biology techniques, site-directed mutagenesis, genetic engineering or polymerase chain reaction, such as, by cloning and expressing a nucleotide molecule encoding a CT-CRM protein with optional other immunogens and optional carrier proteins within a host microorganism, etc. utilizing the information provided herein (See, e.g., Sambrook et al., Molecular Cloning. A Laboratory Manual, 2d Edit., Cold Spring Harbor Laboratory, New York (1989); Ausubel et al. Current Protocols in Molecular Biology, John Wiley & Sons, New York (1997)). Coding sequences for the CT-CRMs and optional immunogens can be prepared synthetically (W. P. C. Stemmer et al, 1995 Gene, 164:49).
In general, recombinant DNA techniques involve obtaining by synthesis or isolation a DNA sequence that encodes the CT-CRM protein as described above, and introducing it into an appropriate vector/host cell expression system where it is expressed, preferably under the control of an arabinose inducible promoter. Any of the methods described for the insertion of DNA into an expression vector may be used to ligate a promoter and other regulatory control elements into specific sites within the selected recombinant vector. Suitable host cells are then transformed, infected, transduced or transfected with such vectors or plasmids by conventional techniques.
A variety of host cell-vector (plasmid) systems may be used to express the immunogenic mutant cholera holotoxil The vector system, which preferably includes the arabinose inducible promoter, is compatible with the host cell used. The DNA encoding the mutant CT-CRMs are inserted into an expression system, and the promoter (preferably the arabinose inducible promoter), and other control elements are ligated into specific sites within the vector so that when the vector is inserted into a host cell (by transformation, transduction or transfection, depending on the host cell-vector system used) the DNA encoding the CT-CRM is expressed by the host cell.
The vector may be selected from one of the viral vectors or non-viral vectors described above but must be compatible with the host cell used. The recombinant DNA vector may be introduced into appropriate host cells (bacteria, virus, yeast, mammalian cells or the like) by transformation, transduction or transfection (depending upon the vector/host cell system). Host-vector systems include but are not limited to bacteria transformed with bacteriophage DNA, plasmid DNA or cosmid DNA; microorganisms such as yeast containing yeast vectors; mammalian cell systems infected with virus (e.g., vaccinia virus, adenovirus, etc.); and insect cell systems infected with virus (e.g., baculovirus).
Systems for cloning and expressing the CT-CRMs and other compositions of this invention using the synthetic nucleic acid molecules include the use of various microorganisms and cells that are well known in recombinant technology. The host cell may be selected from any biological organism, including prokaryotic (e.g., bacterial) cells and eukaryotic cells, including, mammalian, insect cells, yeast cells. Preferably, the cells employed in the various methods and compositions of this invention are bacterial cells. Suitable bacterial cells include, for example, various strains of E. coli, Bacillus, and Streptomyces. Yeast cells such as Saccharomyces and Pichia, and insect cells such as Sf9 and Sf21 cells are also useful host cells for production purposes. Mammalian cells including but not limited to Chinese hamster ovary cells (CHO), chick embryo fibroblasts, baby hamster kidney cells, NIH3T3, PER C6, NSO, VERO or COS cells are also suitable host cells, as well as other conventional and non-conventional organisms and plants.
The selection of other suitable host cells and methods for transformation, culture, amplification, screening and product production and purification can be performed by one of skill in the art by reference to known techniques. See, e.g., Gething and Sambrook, 1981 Nature, 293:620-625, among others.
Typically, the host cell is maintained under culture conditions for a period of time sufficient for expression Culture conditions are well known in the art and include ionic composition and concentration, temperature, pH and the like. Typically, transfected cells are maintained under culture conditions in a culture medium Suitable media for various cell types are well known in the art. In a preferred embodiment, temperature is from about 20° C. to about 50° C., more preferably from about 30° C. to about 40° C. and, even more preferably about 37° C.
The pH is preferably from about a value of 6.0 to a value of about 8.0, more preferably from about a value of about 6.8 to a value of about 7.8 and, most preferably about 7.4. Osmolality is preferably from about 200 milliosmoles per liter (mosm/L) to about 400 mosm/l and, more preferably from about 290 mosm/L to about 310 mosm/L. Other biological conditions needed for transfection and expression of an encoded protein are well known in the art.
Recombinant CT-CRM protein is recovered or collected either from the host cells or membranes thereof or from the medium in which those cells are cultured Recovery comprises isolating and purifying the recombinant CT-CRM protein. Isolation and purification techniques for polypeptides are well known in the art and include such procedures as precipitation, filtration, chromatography, electrophoresis and the like.
When produced by conventional recombinant means, CT-CRMs of this invention may be isolated and purified from the cell or medium thereof by conventional methods, including chromatography (e.g., ion exchange, affinity, and sizing column chromatography), centrifugation, differential solubility, or by any other standard techniques for the purification or proteins. Several techniques exist for purification of heterologous protein from prokaryotic cells. See, U.S. Pat. Nos. 4,518,526; 4,599,197; and 4,734,362. The purified preparation however produced should be substantially free of host toxins, which might be harmful to humans. In particular, when expressed in gram negative bacterial host cells such as E. coli, the purified peptide or protein should be substantially free of endotoxin contamination See, e.g., Sambrook et al., Molecular Cloning. A Laboratory Manual., 2d Edit., Cold Spring Harbor Laboratory, New York (1989).
The CT-CRMs used in methods and compositions of the invention are not limited to products of any of the specific exemplary processes listed herein. In fact, the protein may be prepared by the methods in the texts cited immediately above or by methods of the texts cited elsewhere in this specification. It is within the skill of the art to isolate and produce recombinantly or synthetically protein compositions for such use.
The four exemplary CT-CRMs of Table 1, two bearing a single amino acid substitution and two bearing double amino acid substitutions were generated as described in detail in Example 1 using some of the methods described above. Specifically, a set of mutant CT clones (CT-CRMs) were generated in E. coli by standard site-directed mutagenesis protocols on plasmids encoding the known CT holotoxin molecules.
It has previously been shown that the resulting yield of purified CT-CRME29H holotoxin was approximately 50 μg per liter of culture medium (see International patent publication No. WO 00/18434). Initial attempts to increase CT-CRME29H yield via modifications to the original plasmid, showed little or no effect. A moderate increase in yield was achieved through co-expression of the plasmid pIIB29H, and derivatives, with Vibrio cholerae DsbA and E. coli RpoH. Co-expression and purification modifications increased the yield of CT-CRME29H to approximately 2 mg/liter.
In order to increase the expression of CT-CRMs of the present invention, the lactose inducible promoter in the plasmids was replaced with an arabinose inducible promoter (Invitrogen Corporation, Carlsbad, Calif.), which was operatively linked to the DNA sequence encoding the CT-CRMs. During cloning it was determined that plasmid pIIB29H contained a ctxA gene encoding CT subunit A from Vibrio cholerae strain 569B, linked to a ctxB gene encoding CT subunit B from Vibrio cholerae strain 2125. Cross alignment of these genes indicated seven base substitutions between the two ctxB genes and a single base change between the ctxA genes. Several of these base substitutions led to amino acid changes in the mature subunits. Of special note is the substitution between the cad genes which leads to an amino acid change within the A-2 portion, or the holotoxin assembly domain of the A subunit. It was not known whether the heterogeneity between these genes had a negative impact on toxin expression or holotoxin assembly. However, it was thought preferable from an evolutionary standpoint that both toxin subunit genes originate from the same source. As such, both the ctxA and ctxB genes used in the construction of the arabinose inducible system originated from Vibrio cholerae strain 569B. The construction of plasmids pLP903, pLP904, pLP905, pLP906, is described in Example 1. The immunogenic mutant cholera holotoxin is produced by transforming, infecting, transducing or transfecting a host cell with a plasmid described above, and culturing the host cell under conditions that permit the expression of said recombinant immunogenic detoxified protein by the host cell Production of CT-CRMs from pLP903, pLP904, pLP905 and pLP906 is approximately 10 mg of purified material per liter of culture.
The resulting CT-CRM protein or nucleic acid molecule may be formulated into an immunogenic composition with any number of selected antigens and screened for adjuvant efficacy by in vivo assays, such as those described in the examples below.
D. Immunogenic Compositions
An effective immunogenic composition according to the invention is one comprising a mutant cholera holotoxin of this invention. Preferably the mutant cholera holotoxin CT-CRM has reduced toxicity compared to a wild-type cholera holotoxin This “reduced toxicity” enables each mutant to be used as an adjuvant in an immunogenic composition without causing significant side effects, particularly those known to be associated with wild-type CT, e.g., diarrhea. More preferably, the CT-CRM in the immunogenic composition of this invention has a single amino acid substitution at the amino acid position 16 or 72 in the A subunit of the holotoxin, or a double amino acid substitution at amino acid positions 16 and 68 or 68 and 72 of the A subunit of the cholera holotoxin. In one embodiment, the CT-CRM may have one or more additional modifications as described above. In another embodiment, the composition comprises a selected antigen and a suitable effective adjuvanting amount of the CT-CRM, wherein said holotoxin significantly enhances the immune response in a vertebrate host to said antigen. The compositions of the present invention modulate the immune response by improving the vertebrate host's antibody response and cell-mediated immune responses to the administration of a composition comprising a selected antigen as described above.
As used herein, the term “effective adjuvanting amount” means a dose of one of the CT-CRM mutants of this invention that is effective in eliciting an increased immune response in a vertebrate host. In a more specific definition, the term “effective adjuvanting amount” means a dose of one of the four CT-CRM mutants described herein (CT-CRMI16A, CT-CRMV72Y, CT-CRMI16A,S68Y, CT-CRMS68Y,V72Y), effective in eliciting an increased immune response in a vertebrate host. Specifically, the CT-CRMs disclosed herein augment mucosal and systemic immune responses following intranasal administration of disparate antigens. Furthermore, even in the presence of pre-existing anti-CT immune responses, the mutant CT-CRMs were able to serve as efficient mucosal adjuvants. The immunogenic mutant CT-CRMs according to the present invention exhibit a balance of reduced toxicity and retained adjuvanticity, such that the resulting mutant CT protein functions as an adjuvant while being tolerated safely by the vertebrate host to which it is introduced. The particular “effective adjuvanting dosage or amount” will depend upon the age, weight and medical condition of the host, as well as on the method of administration. Suitable doses are readily determined by persons skilled in the art.
The immunogenic compositions containing as an adjuvant the mutant cholera holotoxins of this invention also contain at least one antigen selected from among a wide variety of antigens. The antigen(s) may comprise a whole cell or virus, or one or more saccharides, proteins, protein subunits, polypeptide, peptide or fragments, poly- or oligonucleotides, or other macromolecular components. If desired, the antigenic compositions may contain more than one antigen from the same or different pathogenic microorganisms.
Thus, in one embodiment, the immunogenic compositions of this invention comprise as the selected antigen a polypeptide, peptide or fragment derived from a pathogenic bacterium Desirable bacterial immunogenic compositions including the CT-CRM mutants as an adjuvant include those directed to the prevention and/or treatment of disease(s) caused by, without limitation, Haemophilus influenzae (both typable and nontypable), Haemophilus somnus, Moraxella catarrhalis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus faecalis, Helicobacter pylori, Neisseria meningitidis, Neisseria gonorrhoeae, Chlamydia trachomatis, Chlamydia pneumoniae, Chlamydia psittaci, Bordetella pertussis, Alloiococcus otiditis, Salmonella typhi, Salmonella typhimurium, Salmonella choleraesuis, Escherichia coli, Shigella, Vibrio cholerae, Corynebacterium diphtheriae, Mycobacterium tuberculosis, Mycobacterium avium-Mycobacterium intracellulare complex, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Staphylococcus epidermidis, Clostridium tetani, Leptospira interrogans, Borrelia burgdorferi, Pasteurella haemolytica, Pasteurella multocida, Actinobacillus pleuropneumoniae and Mycoplasma gallisepticum. 
In another embodiment, the immunogenic compositions of this invention comprise as the selected antigen a polypeptide, peptide or fragment derived from a pathogenic virus. Desirable viral immunogenic compositions including the CT-CRM mutants as an adjuvant include those directed to the prevention and/or treatment of disease caused by, without limitation, Respiratory syncytial virus, Parainfluenza virus types 1-3, Human metapneumovirus, Influenza virus, Herpes simplex virus, Human cytomegalovirus, Human immunodeficiency virus, Simian immunodeficiency virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Human papillomavirus, Poliovirus, rotavirus, caliciviruses, Measles virus, Mumps virus, Rubella virus, adenovirus, rabies virus, canine distemper virus, rinderpest virus, avian pneumovirus (formerly turkey rhinotracheitis virus), Hendra virus, Nipah virus, coronavirus, parvovirus, infectious rhinotracheitis viruses, feline leukemia virus, feline infectious peritonitis virus, avian infectious bursal disease virus, Newcastle disease virus, Marek's disease virus, porcine respiratory and reproductive syndrome virus, equine arteritis virus and various Encephalitis viruses.
In another embodiment, the immunogenic compositions of this invention comprise as the selected antigen a polypeptide, peptide or fragment derived from a pathogenic fungus. Desirable immunogenic compositions against fungal pathogens including the CT-CRM mutants as an adjuvant include those directed to the prevention and/or treatment of disease(s) caused by, without limitation, Aspergillis, Blastomyces, Candida, Coccidiodes, Cryptococcus and Histoplasma. 
In still another embodiment, the immunogenic compositions of this invention comprise as the selected antigen a polypeptide, peptide or fragment derived from a pathogenic parasite. Desirable immunogenic compositions against parasites including the CT-CRM mutants as an adjuvant include those directed to the prevention and/or treatment of disease(s) caused by, without limitation, Leishmania major, Ascaris, Trichuris, Giardia, Schistosoma, Cryptosporidium, Trichomonas, Toxoplasma gondii and Pneumocystis carinii. 
Desirable immunogenic compositions directed against non-infectious diseases including the CT-CRM mutants as an adjuvant are also within the scope of this invention. Such immunogenic compositions include those directed to vertebrate antigens, particularly compositions directed against antigens for the prevention and/or treatment of disease(s), without limitation, such as allergy, autoimmune disease, Alzheimer disease and cancer.
For example, the immunogenic composition of this invention may contain a polypeptide, peptide or fragment derived from a cancer cell or tumor cell Desirable immunogenic compositions for eliciting a therapeutic or prophylactic anti-cancer effect in a vertebrate host, which contain the CT-CRM mutants of this invention, include those utilizing a cancer antigen or tumor-associated antigen including, without limitation, prostate specific antigen, carcino-embryonic antigen, MUC-1, Her2, CA-125, MAGE-3, hormones, hormone analogs and so forth.
Other immunogenic compositions of this invention are desirable for moderating responses to allergens in a vertebrate host. Such compositions contain the CT-CRM mutant(s) of this invention and a polypeptide, peptide or fragment derived from-an allergen or fragment thereof Examples of such allergens are described in the U.S. Pat. No. 5,830,877 and International patent publication No. WO 99/51259, which are hereby incorporated by reference, and include pollen, insect venoms, animal dander, fungal spores and drugs (such as penicillin). The immunogenic compositions interfere with the production of IgE antibodies, a known cause of allergic reactions, so as to moderate allergic responses to the allergen.
In still another embodiment, the immunogenic compositions of this invention contain as the selected antigen a polypeptide, peptide or fragment derived from a molecular portion of an antigen, which represents those produced by a host (a self molecule) in an undesired manner, amount or location, such as those from amyloid precursor protein so as to prevent or treat disease characterized by amyloid deposition in a vertebrate host. Desirable compositions for moderating responses to self molecules in a vertebrate host, which contain CT-CRM mutants of this invention, include those containing a self molecule or fragment thereof Examples of such self molecules include β-chain insulin involved in diabetes, the G17 molecule involved in gastroesophageal reflux disease, and antigens which downregulate autoimmune responses in diseases such as multiple sclerosis, lupus and rheumatoid arthritis.
Still other immunogenic compositions of this invention are desirable for preventing or treating disease characterized by amyloid deposition in a vertebrate host. Such compositions contain the CT-CRM mutant(s) of this invention as well as portions of amyloid precursor protein (APP). This disease is referred to variously as Alzheimer's disease, amyloidosis or amyloidogenic disease. The β-amyloid peptide (also referred to as Aβ peptide) is a 42 amino acid fragment of APP, which is generated by processing of APP by the β and γ secretase enzymes, and has the following sequence: Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys Leu Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile Gly Leu Met Val Gly Gly Val Val Ile Ala (SEQ ID NO: 3). In some patients, the amyloid deposit takes the form of an aggregated Aβ peptide. Surprisingly, it has now been found that administration of isolated Aβ peptide induces an immune response against the Aβ peptide component of an amyloid deposit in a vertebrate host (International patent publication No. WO 99/27944). Thus, embodiments of this invention include the CT-CRM mutants of this invention plus Aβ peptide, as well as fragments of Aβ peptide and antibodies to Aβ peptides or fragments thereof One such fragment of Aβ peptide is the 28 amino acid peptide having the following sequence (U.S. Pat. No. 4,666,829): Asp Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys Leu Val Phe Phe Ala Gln Asp Val Gly Ser Asn Lys (SEQ ID NO: 4).
Such immunogenic compositions further comprise an immunologically acceptable diluent or a pharmaceutically acceptable carrier, such as sterile water or sterile isotonic saline. The antigenic compositions may also be mixed with such diluents or carriers in a conventional manner. As used herein the language “pharmaceutically acceptable carrier” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with administration to humans or other vertebrate hosts. The appropriate carrier will be evident to those skilled in the art and will depend in large part upon the route of administration.
The immunogenic compositions may also include, but are not limited to, suspensions, solutions, emulsions in oily or aqueous vehicles, pastes, and implantable sustained-release or biodegradable formulations. Such formulations may further comprise one or more additional ingredients including, but not limited to, suspending, stabilizing, or dispersing agents. In one embodiment of a formulation for parenteral administration, the active ingredient is provided in dry (i.e., powder or granular) form for reconstitution with a suitable vehicle (e.g., sterile pyrogen-free water) prior to parenteral administration of the reconstituted composition. Other parenterally-administrable formulations, which are useful, include those, which comprise the active ingredient in microcrystalline form, in a liposomal preparation, or as a component of a biodegradable polymer system Compositions for sustained release or implantation may comprise pharmaceutically acceptable polymeric or hydrophobic materials such as an emulsion, an ion exchange resin, a sparingly soluble polymer, or a sparingly soluble salt.
Still additional components that may be present in the protein immunogenic compositions of this invention are adjuvants in addition to the CT-CRMs, preservatives, chemical stabilizers, or other antigenic proteins. Typically, stabilizers, adjuvants, and preservatives are optimized to determine the best formulation for efficacy in the target human or animal. Suitable exemplary preservatives include chlorobutanol, potassium sorbate, sorbic acid, sulfur dioxide, propyl gallate, the parabens, ethyl vanillin, glycerin, phenol, and parachlorophenol. Suitable stabilizing ingredients that may be used include, for example, casamino acids, sucrose, gelatin, phenol red, N-Z amine, monopotassium diphosphate, lactose, lactalbumin hydrolysate, and dried milk.
The antigenic compositions of this invention may comprise further adjuvants in addition to the mutant CT-CRMs. A conventional non-CT-CRM adjuvant used to enhance an immune response include, without limitation, MPLTM (3-O-deacylated monophosphoryl lipid A; Corixa, Hamilton, Mont.), which is described in U.S. Pat. No. 4,912,094, which is hereby incorporated by reference. Also suitable for use as adjuvants are synthetic lipid A analogs or aminoalkyl glucosamine phosphate compounds (AGP), or derivatives or analogs thereof which are available from Corixa (Hamilton, Mont.), and which are described in U.S. Pat. No. 6,113,918, which is hereby incorporated by reference. One such AGP is 2-[(R)-3-Tetradecanoyloxytetradecanoylamino] ethyl 2-Deoxy-4-O-phosphono-3-O-[(R)-3-tetradecanoyoxytetradecanoyl]-2-[(R)-3-tetradecanoyoxytetradecanoyl-amino]-b-D-glucopyranoside, which is also known as 529 (formerly known as RC529). This 529 adjuvant is formulated as an aqueous form or as a stable emulsion.
Still other non-CT-CRM adjuvants include mineral oil and water emulsions, aluminum salts (alum), such as aluminum hydroxide, aluminum phosphate,-etc., Amphigen, Avridine, L121/squalene, D-lactide-polylactide/glycoside, pluronic polyols, muramyl dipeptide, killed Bordetella, saponins, such as StimulonTM QS-21 (Antigenics, Framingham, Mass.), described in U.S. Pat. No. 5,057,540, which is hereby incorporated by reference, and particles generated therefrom such as ISCOMS (immunostimulating complexes), Mycobacterium tuberculosis, bacterial lipopolysaccharides, synthetic polynucleotides such as oligonucleotides containing a CpG motif (U.S. Pat. No. 6,207,646, which is hereby incorporated by reference), a pertussis toxin (PT), or an E. coli heat-labile toxin (LT), particularly LT-K63, LT-R72, CT-S109, PT-K9/G129; see, e.g., International Patent Publication Nos. WO 93/13302 and WO 92/19265, incorporated herein by reference.
Various cytokines and lymphokines are also suitable for inclusion in the immunogenic compositions of this invention. One such cytokine is granulocyte-macrophage colony stimulating factor (GM-CSF), which has a nucleotide sequence as described in U.S. Pat. No. 5,078,996, which is hereby incorporated by reference. A plasmid containing GM-CSF cDNA has been transformed into E. coli and has been deposited with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209, under Accession No. 39900. The cytokine Interleukin-12 (IL-12) is another adjuvant that is described in U.S. Pat. No. 5,723,127, which is hereby incorporated by reference (available from Genetics Institute, Inc., Cambridge, Mass.). Other cytokines or lymphokines have been shown to have immune modulating activity, including, but not limited to, the interleukins 1-α, 1-β, 2, 4, 5, 6, 7, 8, 10, 13, 14, 15, 16, 17 and 18, the interferons-α, β and γ, granulocyte colony stimulating factor, and the tumor necrosis factors α and β, and are suitable for use as adjuvants.
Still other suitable optional components of the immunogenic compositions of this invention include, but are not limited to: surface active substances (e.g., hexadecylamine, octadecylamine, octadecyl amino acid esters, lysolecithin, dimethyl-dioctadecylammonium bromide), methoxyhexadecylgylcerol, and pluronic polyols; polyamines, e.g., pyran, dextransulfate, poly IC, carbopol; peptides, e.g., muramyl dipeptide, dimethylglycine, tuftsin; oil emulsions; and mineral gels, e.g., aluminum phosphate, etc. and immune stimulating complexes. The CT-CRM and antigen may also be incorporated into liposomes, or conjugated to polysaccharides, lipopolysaccharides and/or other polymers for use in an immunogenic composition.
Immunogenic compositions of this invention including the CT-CRM mutant(s), or DNA sequences and molecules encoding the desired CT-CRM of this invention, are also useful as polynucleotide compositions (also known as DNA immunogenic compositions) or administered with polynucleotides encoding the selected antigen. For example, it has been previously demonstrated that BALB/c mice administered a formulation of plasmid DNA (pDNA) encoding the full length glycoprotein D of herpes simplex virus (HSV) type 2 (gD2), along with CT-CRME29H by the intradermal route generated a higher average cellular response than those that received plasmid DNA encoding HSV gD2 by itself by the intradermal route. In addition, the average serum antibody titers for mice, which received the plasmid DNA HSV gD2 composition along with CT-CRME29H was approximately the same as that seen in mice that received the plasmid DNA HSV gD2 composition without adjuvant. Similarly, the plasmid DNA HSV gD2 composition adjuvanted with CT-CRME29H also generated a gD2-specific antibody response in vaginal wash samples at levels that were comparable to those seen following the delivery of the non-adjuvanted composition by intradermal or intramuscular routes. Mice immunized with the plasmid DNA HSV gD2 composition adjuvanted with CT-CRME29H or CT and delivered by the intradermal route also generated substantially higher levels of gamma interferon and IL-5 than mice that received the plasmid DNA HSV-gD2 composition without adjuvant. Thus, the CT-CRMs enhance proliferative and gamma interferon responses when administered with a plasmid DNA composition against HSV.
In addition to a carrier as described above, immunogenic compositions composed of polynucleotide molecules desirably contain optional polynucleotide facilitating agents or “co-agents”, such as a local anesthetic, a peptide, a lipid including cationic lipids, a liposome or lipidic particle, a polycation such as polylysine, a branched, three-dimensional polycation such as a dendrimer, a carbohydrate, a cationic amphiphile, a detergent, a benzylammonium surfactant, or another compound that facilitates polynucleotide transfer to cells. Such a facilitating agent includes bupivicaine (see U.S. Pat. No. 5,593,972, which is hereby incorporated by reference). Other non-exclusive examples of such facilitating agents or co-agents useful in this invention are described in U.S. Pat. Nos. 5,703,055; 5,739,118; 5,837,533; International Patent Publication No. WO96/10038, published Apr. 4, 1996; and International Patent Publication No WO94/16737, published August 8, 1994, which are each incorporated herein by reference.
Most preferably, the local anesthetic is present in an amount that forms one or more complexes with the nucleic acid molecules. When the local anesthetic is mixed with nucleic acid molecules or plasmids of this invention, it forms a variety of small complexes or particles that pack the DNA and are homogeneous. Thus, in one embodiment of the immunogenic compositions of this invention, the complexes are formed by mixing the local anesthetic and at least one plasmid of this invention Any single complex resulting from this mixture may contain a variety of combinations of the different plasmids. Alternatively, in another embodiment of the compositions of this invention, the local anesthetic may be pre-mixed with each plasmid separately, and then the separate mixtures combined in a single composition to ensure the desired ratio of the plasmids is present in a single immunogenic composition, if all plasmids are to be administered in a single bolus administration Alternatively, the local anesthetic and each plasmid may be mixed separately and administered separately to obtain the desired ratio. Where, hereafter, the term “complex” or “one or more complexes” or “complexes” is used to define this embodiment of the immunogenic composition, it is understood that the term encompasses one or more complexes with each complex containing a mixture of the CT-CRM-encoding plasmids and antigen-encoding plasmids, or a mixture of complexes formed discretely, wherein each complex contains only one type of plasmid, or a one or a mixture of complexes wherein each complex contains a polycistronic DNA. Preferably, the complexes are between about 50 to about 150 nm in diameter. When the facilitating agent used is a local anesthetic, preferably bupivacaine, an amount of from about 0.1 weight percent to about 1.0 weight percent based on the total weight of the polynucleotide composition is preferred. See, also, International Patent Publication No. WO99/21591, which is hereby incorporated by reference, and which teaches the incorporation of benzylammonium surfactants as co-agents, preferably administered in an amount of between about 0.001-0.03 weight %. According to the present invention, the amount of local anesthetic is present in a ratio to said nucleic acid molecules of 0.01-2.5% w/v local anesthetic to 1-10 μg/ml nucleic acid. Another such range is 0.05-1.25% w/v local anesthetic to 100 μg/ml to 1 ml/ml nucleic acid.
As used, such a polynucleotide immunogenic composition expresses the CT-CRM and antigens on a transient basis in vivo; no genetic material is inserted or integrated into the chromosomes of the host. This use is thus distinguished from gene therapy, where the goal is to insert or integrate the genetic material of interest into the chromosome. An assay is used to confirm that the polynucleotides administered by immunization do not rise to a transformed phenotype in the host (U.S. Pat. No. 6,168,918).
The immunogenic compositions may also contain other additives suitable for the selected mode of administration of the composition. The composition of the invention may also involve lyophilized polynucleotides, which can be used with other pharmaceutically acceptable excipients for developing powder, liquid or suspension dosage forms. See, e.g., Remington: The Science and Practice of Pharmacy, Vol. 2, 19th edition (1995), e.g., Chapter 95 Aerosols; and International Patent Publication No. WO99/45966, the teachings of which are hereby incorporated by reference. Routes of administration for these compositions may be combined, if desired, or adjusted.
These nucleic acid molecule-containing immunogenic compositions can contain additives suitable for administration via any conventional route of administration. In some preferred embodiments, the immunogenic composition of the invention is prepared for administration to human subjects in the form of for example, liquids, powders, aerosols, tablets, capsules, enteric-coated tablets or capsules, or suppositories.
The immunogenic compositions of the present invention (whether protein-containing or nucleic acid molecule-containing compositions), as described above, are not limited by the selection of the conventional, physiologically acceptable, carriers, adjuvants, or other ingredients useful in pharmaceutical preparations of the types described above. The preparation of these pharmaceutically acceptable compositions, from the above-described components, having appropriate pH isotonicity, stability and other conventional characteristics is within the skill of the art.
E. Methods of Use of the Compositions of this Invention
The immunogenic compositions of this invention that comprise the CT-CRM alone or a combination of the CT-CRM and a selected antigen, are administered to a human or to a non-human vertebrate by a variety of routes to enhance the immune response to an antigen, preferably a disease-causing antigen, as identified above. The compositions of the present invention modulate the immune response by improving the vertebrate host's antibody response and cell-mediated immunity after administration of a composition comprising a selected antigen as described above, and an effective adjuvanting amount of a mutant CT-CRM where the mutant CT-CRM has substantially reduced toxicity compared to a wild-type CT, and wherein the reduced toxicity is a result of a single amino acid substitution, a double amino acid substitution, or amino acid insertions.
In one embodiment, the immunogenic composition containing the CT-CRM (either as a protein or encoded by a nucleic acid molecule) is administered prior to administration of a composition comprising the selected antigen (either as a protein or as a nucleic acid). In another embodiment, the immunogenic composition is administered simultaneously with the antigen, whether it is administered in a composition containing both antigen and CT-CRM or as a separate composition from that of the antigen-containing composition In still a further embodiment, the composition containing the CT-CRM is administered after the composition containing the antigen. It is preferable, although not required, that the antigen and the mutant CT-CRM be administered at the same time.
The immunogenic composition containing the CT-CRM may be administered as a protein or as a nucleic acid molecule encoding the protein, as described above. The immunogenic composition containing the CT-CRM may be administered as a protein in combination with a selected antigen administered as a protein. Alternatively, as described above, the CT-CRM immunogenic composition may be administered as a protein with a nucleic acid molecule encoding the antigen, as described above. Still another alternative involves administering both the CT-CRM and the antigen as nucleic acid sequences encoding these proteins.
Any suitable route of administration may be employed to administer the immunogenic composition containing the CT-CRM. The route may be the same or different from a route selected to administer a composition containing the selected antigen, if the CT-CRM and antigen are administered in separate compositions or in different forms, e.g., protein or nucleic acids. Suitable routes of administration include, but are not limited to, intranasal, oral, vaginal, rectal, parenteral, intradermal, transdermal (see, e.g., International patent publication No. WO 98/20734, which is hereby incorporated by reference), intramuscular, intraperitoneal, subcutaneous, intravenous and intraarterial. The appropriate route is selected depending on the nature of the immunogenic composition used, and an evaluation of the age, weight, sex and general health of the patient and the antigens present in the immunogenic composition, and similar factors by an attending physician
In general, selection of the appropriate “effective amount” or dosage for the the CT-CRM and/or antigen components of the immunogenic composition(s) of the present invention will also be based upon the protein or nucleic acid form of the CT-CRM and antigen, the identity of the antigen in the immunogenic composition(s) employed, as well as the physical condition of the subject, most especially including the general health, age and weight of the immunized subject. The method and routes of administration and the presence of additional components in the immunogenic compositions may also affect the dosages and amounts of the CT-CRM and antigen. Such selection and upward or downward adjustment of the effective dose is within the skill of the art. The amount of CT-CRM and antigen required to induce an immune response, preferably a protective response, or produce an exogenous effect in the patient without significant adverse side effects varies depending upon these factors. Suitable doses are readily determined by persons skilled in the art.
As an example, in one embodiment, for the compositions containing protein components, e.g., a CT-CRM variant protein and/or antigen as described above, each dose may comprise between about 1 μg to about 20 mg of the protein per mL of a sterile solution. Other dosage ranges may also be contemplated by one of skill in the art. Initial doses may be optionally followed by repeated boosts, where desirable. In another example, the amounts of nucleotide molecules in the DNA and vector compositions may be selected and adjusted by one of skill in the art. In one embodiment, each dose will comprise between about 50 μg to about 1 mg of CT-CRM-encoding or antigen-encoding nucleic acid, e.g., DNA plasmid, per mL of a sterile solution.
The number of doses and the dosage regimen for the composition are also readily determined by persons skilled in the art. Protection may be conferred by a single dose of the immunogenic composition containing the CT-CRM, or may require the administration of several doses with or without the selected antigen, in addition to booster doses at later times to maintain protection. In some instances, the adjuvant property of the mutant CT-CRM may reduce the number of doses containing antigen that are needed or may reduce the time course of the dosage regimen. The levels of immunity can be monitored to determine the need, if any, for boosters.
In order that this invention may be better understood, the following examples are set forth. The examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention.
All references cited herein are hereby incorporated by reference.
EXAMPLE 1 
Expression of CT Mutants
A. Bacterial Strains, Plasmids and Growth Conditions
E. coli TG1 (Amersham-Pharmacia Biotech, Piscataway, N.J.), and TX1, a nalidixic acid-resistant derivative of TG1, carrying FTc,lacIq from XL1 blue (Stratagene, LaJolla, Calif.; and CJ236(FTc, lacIq) (Bio-Rad, Hercules, Calif.) were used as hosts for cloning recombinant plasmids and expression of mutated proteins. Plasmid-containing strains were maintained on LB agar plates with antibiotics as required (ampicillin, 50 μg/ml; kanamycin 25 μg/ml; tetracycline 10 μg/ml). A complete CT operon from V. cholerae 0395 was subcloned into the phagemid vector pSKII-, under the control of the lac promoter, to create the IPTG inducible plasmid designated pMGJ67 (Jobling, M. G., and Holmes, R. K., 1992 Infect. Immun., 60, 4915-4924).
B. Mutagenesis of ctxA Gene
The method of Kunkel, T. A,1985 Proc. Natl. Acad. Sci., USA, 82, 488-492 was used to select for oligonucleotide-derived mutants created in plasmid pMGJ67. The oligonucleotides used to generate the mutant CT-CRMs and the various amino acid substitutions in the mutant CT-CRMs are listed in Table 2.







TABLE 2







Sequence of Oligonucleotides Introduced into ctxA












SEQ



Substitution
Oligonucleotide Sequencea
ID NO.





I16A
CCTCCTGATGAAGSYCAAGCAGTCAGG
5



 


S68Y
GTTTGAGATCTGCCCACT
6


 


V72Y
GTTTGACCCACTAAGTGGGC
7


 


S68Y + V72Y
GTTTGAGATATGCCCACTTATATGGTCAAC
8





aAltered bases are underlined. S represents G or C; Y represents C or T.






Briefly, the CT-CRMI16A mutant was made directly in pMGJ142 using the QuickChange mutagenesis kit as described by the supplier (Stratagene Inc., LaJolla, Calif.). The double mutant plasmid containing the CT-CRMS68Y,V72Y substitutions was made by PCR using the mutagenic primer disclosed in Table 2 to create a megaprimer followed by cloning of the mutated ctxA-encoding XbaI-ClaI fragment into pMGJ142. The CT-CRMI16A,S68Y double mutant was made by PCR of the I16A containing clone using the mutagenic primer to create a megaprimer followed by cloning of the mutated ctxA-encoding XbaI-ClaI fragment into pMGJ142. The CT-CRMV72Y and CT-CRMI16A,S68Y mutants were made by reversion of the CT-CRMS68Y,V72Y double mutant back to wild-type at amino acid position 68 using the QuickChange mutagenesis kit. Each single-stranded oligonucleotide was phosphorylated and used to direct second strand synthesis on a uracil-containing single-stranded DNA template rescued from the E. coli dut ung strain CJ236 (F′Tc, pMGJ67). Following ligation and transformation of ung+ strain TX1, single-stranded DNA was rescued from AmpR transformants and sequenced by the dideoxy chain termination method (Kunkel, cited above).
C. Construction of Arabinose Promoted CT-CRM Expression Vectors
Previous experience with CT-CRME29H International patent publication No. WO 00/18434) has shown that maximal production in E. coli could be achieved by substituting synthetic Shine-Delgarno sequences upstream of the ctxA gene and placing the operon under the control of the arabinose promoter system CT operons containing site directed mutations in the A subunit were made as previously described (supra). CT-CRMs were originally under the control of a βlac promoter and expression levels in E. coli were low. PCR was used to modify the region 5′ to the ATG of the CT-A subunit and insert an NheI site at the 5′ end. The corresponding 3′ primer added a HindIII site at the 3′ end of the CT-B gene.








Primer sequences used were:









(SEQ. ID NO. 9)










CT forward:
5′ TTTTTTGGGCTAGCATGGAGGAAAAGATGAGC;



and


 








(SEQ. ID NO. 10)










CT reverse:
5′ CGAGGTCGAAGCTTGCATGTTTGGGC.







PCR was performed on each mutant CT-CRM operon and the PCR products were ligated into pCR2.1-Topo (Invitrogen) according to the manufacturer's directions and transformed into Top10F′ cells. Recombinant E. coli were plated onto SOB agar containing Kanamycin (25 μg/ml) and X-gal (40 μg/ml). Plasmids from white colonies were screened for inserts by digestion with EcoRI. Plasmids containing inserts of the correct size were digested with NheI and HindIII according to the manufacturer's directions and the DNA fragments containing the CT operons isolated from low melting point agarose. Plasmid pBAD18-Cm (Invitrogen) was digested with NheI-HindIII and the linear DNA isolated from low melting point agarose. Digested pBAD18 and the CT operons were ligated at 12° C. and transformed into Top10F E. coli. Plasmids from chloramphenicol-resistant colonies were screened for inserts by restriction analysis, and representative clones were sequenced to confirm the presence of the site directed mutations. Plasmids were transformed into DH5α for expression of CT-CRMs. The plasmids encoding the mutant CT-CRMs bearing a single amino acid substitution at positions 16 and 72 are designated as pLP903 and pLP905 respectively, and the plasmids encoding the mutant CT-CRMs bearing double amino acid substitution at positions 16 and 68 and at positions 68 and 72 are designated as pLP904 and pLP906 respectively. The plasmids contain the polycistron of V. cholerae genes ctxA and ctxB which encode the CT.
D. Expression of CT-CRMs in E. coli 
E. coli DH5α cell s containing plasmids pLP903, pLP904, pLP905, or pLP906, cells expressing CT-CRMs CT-CRMI16A, CT-CRMS68Y,I16A, CT-CRMV72Y, and CT-CRMS68Y,V72Y respectively, were grown in phosphate buffered Hy-Soy media containing chloramphenicol (25 μg/ml) and glycerol (0.5%) at 37° C. with aeration When cultures reached an OD600 of approximately 4.5-5.5, they were induced by addition of L-arabinose to a final concentration of 0.5%. Cultures were incubated at 37° C. with aeration for three hours post-induction and then the cells collected by centrifugation Cell pellets were stored at −20° C.
E. Preparation and Purification of CT-CRMs
Cell pellets were thawed at room temperature and resuspended in 10 mM NaPO4 and 1 mM EDTA (pH 7.0) at 9% of the original culture volume. Cell suspensions were mechanically disrupted in a microfluidizer and centrifuged for 10 minutes at 8,500×g. Cell lysates were further clarified at 160,000×g for one hour. The clarified cell lysate was loaded, at a flow rate of 2 ml/min, onto a carboxymethyl (CM)-sepharoseTM column (300 ml CM-sepharoseTM per 10 l of culture) (Amersham, Pharmacia) equilibrated with 10 mM NaPO4 (pH 7.0). The column was washed with >10 volumes of 10 mM NaPO4 (pH 7.0) at a flow rate of 5 ml/min. CT-CRME29H holotoxin was eluted with four column volumes of 10 mM NaPO4 (pH 8.3). Purified CT-CRMs were buffer exchanged by dialysis into PBS and stored at 4° C. The presence of intact holotoxin and the respective subunits was determined by native polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE, respectively. Native PAGE indicated the presence of a purified molecule of 86 kDa (data not shown), the expected molecular weight for intact cholera holotoxin (Tebbey et al., 2000 Vaccine, 18(24): 2723-2734.
In addition, SDS-PAGE showed two bands that aligned with the CT-A (27 kDa) and CT-B (12 kDa) subunits that comprise the intact holotoxin (data not shown).
EXAMPLE 2 
Non-Denaturing Polyacrylamide Gel Electrophoresis
Mutant CT-CRMS, CT-CRMI16A,S68Y and CT-CRMv72Y, were analyzed by non-denaturing page electrophoresis to determine the percentage of the CT-CRMs present after purification as intact holotoxin Purified CT-CRMs, 15 μl each (at various protein concentrations), were run through a 6% polymerized non-denaturing polyacrylamide gel. Three different concentrations (300, 600 and 1200 ng) of CT-B were used as a standard. After electrophoresis the gel was stained with Coomassie blue. The gel was then scanned using a densitometer, and the percentage of the holotoxin was calculated from the densitometer readings of the CT-CRMs and CT-B standard. The data indicated that 97.8% of CT-CRMI16A,S68Y and 99.4% of CT-CRMV72Y were present as intact holotoxins (Table 3).







TABLE 3







Native Gel Assay for Intact Holotoxin










CT-CRM
% of holotoxin







CT-CRMI16A
97.8



CT-CRMV72Y
99.4



CT-CRMI16A, S68Y
Not done



CT-CRMS68Y, V72Y
Not done










EXAMPLE 3 
Y-1 Adrenal Cell Assay for Residual Toxicity of CT-CRMS
Mutant CT-CRMs were compared with wild-type CT for toxicity in the mouse Y-1 adrenal tumor cell assay, which is used in vitro to measure toxicity of enterotoxins in the cholera toxin/heat labile enterotoxin family. The assay depends upon binding of the toxin to cell surface receptors, and the subsequent entry of the A1 subunit of the toxin into the cytoplasm of the cell.
Native cholera toxin isolated from V. cholerae is proteolytically nicked at the CT-A1- CT-A2 junction, resulting in the A1 and A2 subunits of cholera toxin being held together by only a disulfide bond. This makes the A1 and A2 subunits unstable and easily dissociable from each other. The A1 subunit of the nicked CT dissociates from the A2 subunit upon binding cell surface receptor, and enters the cell, where it ADP-ribosylates the regulatory G-protein (Gsα), leading to its toxic effects as described in the background above. In contrast, enterotoxins produced in E. coli (either CT or LT) are unpicked, and thus, have the A1-A2 peptides still joined. Consequently, the CT produced in V. cholerae are significantly more toxic in the Y-1 adrenal cell assays than the CT produced in a heterologous bacterial cell such as E. coli. 
In a first Y-1 adrenal cell assay, mutant CT-CRMs were compared to nicked wild-type CT from V. cholerae for toxicity. In this assay, Y-1 adrenal cells (ATCC CCL-79) were seeded in 96-well flat-bottom plates at a concentration of 104 cells per well. Thereafter, three-fold serial dilutions of purified ( ̃90% purity as determined by Coomassie staining) CT-CRMs were added to the tumor cells and incubated at 37° C. (5% CO2) for 18 hours. The cells were then examined by light microscopy for evidence of toxicity (cell rounding). The endpoint titer was defined as the minimum concentration of toxin required for greater than 50% cell rounding. The percent of residual toxicity was then calculated using the endpoint titer of wild-type nicked CT from V. cholerae (100% toxicity) divided by the titer- elicited by CT-CRMs multiplied by 100. The data set forth in Table 4 indicate that the residual toxicity of the four purified mutant holotoxins, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMv72Y, and CT-CRMS68Y,V72Y tested using the Y-1 adrenal cell assay was only 0.37%.







TABLE 4







Y-1 Adrenal Cell Assay










CT-CRM
% Residual Toxicity







CT-CRMI16A
0.37



CT-CRMI16A, S68Y
0.37



CT-CRMV72Y
0.37



CT-CRMS68Y, V72Y
0.37










In a second independent study, crude periplasmic extracts of E. coli cells (TG1) expressing elevated levels of mutant CT-CRMs, were compared against unnicked wild-type CT holotoxin expressed in E. coli for residual toxicity in Y-1 adrenal cell assay. Y-1 adrenal cells were incubated in multi-well dishes in an RPMI medium containing 10% fetal calf serum in the presence of crude E. coli cell lysate. Cell toxicity was monitored as before. In this study, one toxic unit was defined as the smallest amount of toxin or supernatant that caused rounding of 75-100% of the cells in a well after overnight incubation. The results of this study are presented in Table 5 below.







TABLE 5







Y-1 Adrenal Cell Assay










CT-CRM
% Residual Toxicity














CT-CRMI16A
5



CT-CRMV72Y
100



CT-CRMS68Y, V72Y
5



CT-CRMI16A, S68Y
Not Determined










The results of this study indicated that while the toxicities of CT-CRMI16A and CT-CRMS68Y,V72Y were substantially reduced (5%), the CT-CRMV72Y was as toxic as the wild-type CT. Without being bound by theory, the variant results in second study (Table 5) may be attributable to the fact that periplasmic crude E. coli cell lysates used in the second study contained unnicked mutant CT-CRMs, and to the fact that the toxicity was measured as a percentage of the toxicity of wild-type, unnicked CT produced by E. coli. In contrast, the unnicked wild-type CT from E. coli has a 50% cell rounding dose of 6250 pg/ml in the Y1 cell assay (data not shown). In the first study, the residual cytotoxicity of the mutant CT-CRMs is expressed as a percentage of the toxicity of wild-type, nicked CT produced by V. cholerae, wherein the nicked holotoxin has a 50% cell rounding dose of 125 pg/ml in the Y1 cell assay. Consequently, the residual toxicity reported in the second study is 50 fold higher than that obtained in the first study.
EXAMPLE 4 
The ADP-Ribosyltransferase Assay
NAD+: agmatine ADP-ribosyltransferase activity was measured as the release of [carbonyl-14C] nicotinamide from radiolabeled NAD+. Briefly, CT and CT-CRMs were trypsin activated and incubated for 30 minutes at 30° C. with 50 mM glycine/20 mM dithiothreitol in TEAN buffer (Tris/EDTA/sodium azide/sodium chloride) (pH 8.0). Thereafter, the following materials were added to the reaction: 0.1 μg of soybean trypsin inhibitor, 50 nM potassium phosphate, 10 mM agmatine, 20 mM dithiothreitol, 10 mM magnesium chloride, 100 μM GTP, 3 mM dimyristoylphosphatidyl-choline, 0.2% cholate, 0.03 mg of ovalbumin, 100 μM [adenine-U-14C]NAD (DuPont NENTM, Boston, Mass.) and water to a final volume of 300 μl. After incubation for 90 minutes at 30° C., 100 μl samples were applied to columns (0.64×5 cm) of AG1-X2 (Bio-Rad) that were washed five times with 1.0 ml of distilled/deionized H2O. Eluates containing [14C]ADP-ribosylagmatine were collected for radioassay. Mean recovery of 14C in the eluate is expressed as percentage of that applied to column. The results are presented in Table 6.







TABLE 6







NAD:Agmatine ADP-Ribosyltransferase Activity










ADP-ribosylagmatine formed
% ADP-ribosylation


CT/CT-CRM
(nmol/hr/μg protein)
activity












CT, 10 μg
52.5
100


CT-CRMI16A
3.3
2.4


CT-CRMI16A, S68Y
3.4
3.3


CT-CRMV72Y
2.7
1.1


CR-CRMS68Y, V72Y
2.9
1.2









ADP-ribosyltransferase activity was also independently determined using diethylamino (benzylidine-amino) guanidine (DEABAG) as a substrate. In this assay, 25 μl aliquots of mutant CT-CRMS from purified cell lysates, activated for 30 minutes at 30° C. with 1/50 w/w trypsin, were incubated with 200 μl 2 mM DEABAG in 0.1M K2PO4, pH 7.5, 10 μM NAD, 4 mM DTT for two hours. The reaction was stopped by adding 800 μl of a slurry buffer containing 400 mg DOWEX AG50-X8 resin, to bind unreacted substrate. ADP-ribosylated DEABAG in the supernatant was quantitated by florescence emission in a DyNA Quant fluorimeter calibrated with DEABAG. With the exception of the mutant CT-CRMV72Y, ADP ribosyl-transferase activities of the mutant CT-CRMs were substantially reduced over that of wild-type (Table 7). The high level of ADP-ribosyl-transferase activity seen with CT-CRMV72Y may be attributable to the fact that in this study the ADP ribosyl-transferase activity of mutant CT-CRMs was measured using a different substrate in a different assay protocol.







TABLE 7







ADP-ribosyltransferase Activity of CT-CRMs using Diethylamino


(benzylidine-amino) Guanidine (DEABAG)










CT/CT-CRM
% ADP-ribosylation Activity














CT
100



CT-CRMS68Y
11



CT-CRMV72Y
68



CT-CRMS68Y, V72Y
3



CT-CRMI16A, S68Y
Not done










EXAMPLE 5 
Immune Responses of BALB/C Mice Immunized with Recombinant P4 Outer Membrane Protein (RP4) of Nontypable Haemophilus Influenzae (NTHI) Alone or in Conjunction with CT-CRMS
In a first experiment, the ability of the mutant CT-CRMI16A to enhance the induction of systemic and mucosal antibodies to recombinant P4 outer membrane protein, (rP4) were assessed Serum and mucosal anti-P4 antibody titers induced by mutant CT-CRMI16A, were assessed and compared with that of wild-type CT and mutant, CT-CRME29H (WO 00/18434). In this study, Balb/c mice were immunized intranasally (IN) at weeks 0, 3 and 5 and at week 5, day 6 with a formulation containing 1 μg of recombinant P4 protein in saline or 1 μg of P4 together with 1 μg of wild-type CT, 1 μg of CT-CRME29H or 0.1, 1, or 10 μg of CT-CRMI16A.
The results indicate that the CT-CRMI16A, like the wild-type CT and CT-CRME29H, augmented the capacity of rP4 protein to elicit systemic and mucosal immune responses (Table 8). For example, six weeks after primary IN immunization the anti-rP4 IgG antibody titers of mice immunuized with rP4 protein formulated with either CT-CRMI16A or CT-CRME29H were 40 times greater than that of mice immunized with the recombinant proteins in PBS alone. The antibody titers (IgG) of mice administered the recombinant proteins plus wild-type CT holotoxin at a concentration of 1 μg were elevated 67-fold in comparison to antibody titers in mice administered recombinant rP4 alone in saline. The antibody titers of mice immunized with 1 μg of the mutant CT-CRM, CT-CRME29H were elevated 55-fold over antibody titers in mice immunized with rP4 alone. In comparison, the antibody titers of mice immunized with 1 μg and 0.1 μg of the mutant CT-CRM4 CT-CRMI16A, were increased 15-fold and 27-fold respectively over the anti-rP4 antibody titers in mice immunized with rP4 alone in saline.







TABLE 8







Serum Antibody Responses to Recombinant P4 Protein












Week 0
Week 3
Week 5
Week 5, Day 6
















Adjuvant
Amount
IgA
IgG
IgA
IgG
IgA
IgG
IgA
IgG




















Saline




148
386
199
394
140
444


CT
1.0
μg


184
1,348
203
1,949
1,570
29,939


CT-CRME29H
1.0
μg


108
615
286
1,057
1,492
21,251


CT-CRMI16A
1.0
μg


<100
426
114
2,071
1,096
6,708


CT-CRMI16A
0.1
μg


261
1,463
403
2,629
1,105
10,541


CT-CRMI16A
10.0
μg
151
502
121
788
108
1,460
183
2,043









An examination of the protein-specific antibodies in the mucosal secretions of the Balb/c mice immunized IN at weeks 0, 3, and 5 was made two weeks after tertiary immunization. Mucosal samples were collected at week 5, day 6 from vaginal wash (VW), the nasal wash (NW); the bronchoalveolar lavage (BAL) and from saliva (SAL). These results shown in Table 9 further indicated the CT-CRMI16A facilitated the generation of local immune responses against the rP4 protein. Moreover, the anti-rP4 antibody titers were comparable to those induced by the wild-type CT adjuvanted immunogenic composition.







TABLE 9







Mucosal Antibody Responses to rP4 Protein.












VW
NW
BAL
SAL
















Adjuvant
Amt
IgA
IgG
IgA
IgG
IgA
IgG
IgA
IgG




















Saline


24
<10
<10
<10
<10
<10
20
<10


CT
1.0
μg
125
74
38
<10
158
152
152
14


CT-CRME29H
1.0
μg
254
12
58
<10
523
364
454
27


CT-CRMI16A
1.0
μg
154
16
39
<10
330
38
654
32


CT-CRMI16A
0.1
μg
422
26
60
<10
125
53
1027
74


CT-CRMI16A
10.0
μg
19
<10
<10
<10
13
16
34
<10









In a second experiment, five BALB/c mice per group were immunized IN on days 0, 21 and 35 with a 15 μl dose containing 1 μg rP4 alone or 1 μg rP4 plus 1 μg of one of the mutant CT-CRMs, CT-CRME29H, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y or CT-CRMS68Y,V72Y as an adjuvant as indicated in Table 10. The anti-rP4 IgA and IgG antibody titers were determined by ELISA on pooled samples collected at weeks 0, 3, 5 and week 5, day 6 and the results shown in Table 10. The results indicate that serum anti-rP4, IgA and IgG titers were substantially increased in mice that were administered the antigen along with one of the mutant CT-CRMS. The mucosal antibody responses to rP4 were also measured one week after the last immunization (week 5, day 6).







TABLE 10







Adjuvant Effects of Mutant Cholera Toxins on the Immune Response


to NTHi rP4 Protein Delivered Intranasally to Female BALB/c Micea









Anti-NTHi rP4 ELISA Endpoint Titers on Pooled Serac












Week 0
Week 3
Week 5
Week 5, day 6















Adjuvantb
IgA
IgG
IgA
IgG
IgA
IgG
IgA
IgG


















Saline
123
593
<100
715
227
1,953
224
6,458


CT-CRME29H


116
934
1,808
135,824
7,365
282,099


CT-CRMI16A


269
423
788
17,465
2,609
143,313


CT-CRMI16A, S68Y


256
545
821
6,062
1,998
438,553


CT-CRMV72Y


294
878
1,725
40,443
5,239
343,711


CT-CRMS68Y, V72Y


172
429
333
4,353
1,333
55,571





a1 μg NTHi rP4 was delivered IN to female BALB/c mice in a 15 μl volume at weeks 0, 3 and 5.


bNTHi rP4 compositions were formulated with saline or 1 μg of various mutant cholera toxins.


cSera were collected at weeks 0, 3, 5 and week 5, day 6; pooled samples represent an n = 5.






Table 11 sets forth the IgA and IgG titers from nasal, bronchoalveolar and vaginal washes, and saliva respectively. These results also indicate that mucosal anti-rP4, IgA and IgG titers were also substantially elevated in mice administered rP4 antigen together with one of the mutant CT-CRMs in comparison to mice administered rP4 in saline.







TABLE 11







Anti-NTHi rP4 ELISA Endpoint Titers on Mucosal Wash Poolsa















Vaginal



Lung Washc
Nasal Washc
Salivac
Washc















Adjuvantb
IgA
IgG
IgA
IgG
IgA
IgG
IgA
IgG


















Saline
<10
65
<10
<10
12
<10
<10
<10


CT-CRME29H
1,131
1,815
196
70
977
1,906
255
149


CT-CRMI16A
360
708
166
37
733
96
1,066
414


CT-CRMI16A, S68Y
449
376
164
19
1,221
177
1,521
261


CT-CRMV72Y
3,850
4,226
500
300
2,753
281
1,066
212


CT-CRMS68Y, V72Y
84
164
111
35
843
103
251
109





a1 μg NTHi rP4 was delivered IN to female BALB/c mice in a 15 μl volume at weeks 0, 3 and 5.


bNTHi rP4 compositions were formulated with saline or 1 μg of various mutant cholera toxins.


cMucosal samples were collected on week 6; pooled samples represent an n = 5.






On week 5, day 6, following IN administration, IgA and IgG including IgG subclass IgGI, IgG2a, IgG2b and IgG3 endpoint titers in the serum of each individual mouse in the six groups were also determined by ELISA. (Tables 12-17). In the data reported in Tables 12-17, statistical analyses were performed using JMP, SAS Institute, Inc.; one-way analysis of variance was significant at the p<0.0001 level; and multiple comparisons were performed using Tukey-Kramer HSD, alpha=0.05.







TABLE 12







IgA Anti-NTHi rP4 ELISA Endpoint Titers in each Individual Mouse


at Week 5, Day 6.















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
217
63
44
48
35
  63
76
34


CT-CRME29H
6,791
2,196
1,740
1,526
4,626
2,835
2,276
1,018


CT-CRMI16A
2,075
2,186
1,208
2,847
1,975
1,985
585
262


CT-CRMI16A, S68Y
1,739
920
739
1,289
5,694
1,541
2,058
921


CT-CRMV72Y
6,523
1,634
1,290
4,089
10,521
 3,584†
3,826
1,711


CT-CRMS68Y, V72Y
1,646
878
310
2,724
116
  676*
1,069
478





*Values differ significantly from the saline group


†Value differs significantly from the CT-CRMs68Y, V72Y group.













TABLE 13







IgG Anti-NTHi rP4 ELISA Endpoint Titers in Each Individual Mouse


at Week 5, Day 6.















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
78,369
2,287
3,505
1,518
1,223
 4,105*
34,105
15,252


CT-CRME29H
440,101
280,030
17,291
111,803
193,831
135,797* 
161,927
72,416


CT-CRMI16A
69,986
92,347
68,406
193,467
62,196
88,141*
54,968
24,582


CT-CRMI16A, s68Y
57,721
59,832
17,521
57,172
29,725
40,034*
19,471
8,708


CT-CRMV72Y
161,796
143,545
72,764
187,118
363,958
163,008* 
108,260
48,415


CT-CRMs68Y, V72Y
80,770
71,053
14,366
73,904
2,658
27,662*
36,953
16,526





*Values differ significantly from the saline group













TABLE 14







IgG1 Anti-NTHi rP4 ELISA Endpoint Titers in


Each Individual Mouse on Week 5, Day 6 Sera.















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
2,533
33
33
33
33
  79
1,118
500


CT-CRME29H
11,462
3,029
1,801
8,517
1,172
3,623*
4,547
2034


CT-CRMI16A
4,732
10,033
3,546
14,399
6,836
6,980*
4,385
1961


CT-CRMI16A, s68Y
2,945
1,036
1,016
2,945
343
1,256*
1,208
540


CT-CRMV72Y
9,123
13,942
545
7,939
35,887
7,230*
13,410
5997


CT-CRMs68Y, V72Y
28,434
13,553
394
7,579
33
2,070*
11,725
5244





*Values differ significantly from the saline group













TABLE 15







IgG2a Anti-NTHi rP4 ELISA Endpoint Titers in Each


Individual Mouse Week 5, Day 6















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
10,611
317
431
230
217
  591
4,613
2063


CT-CRME29H
230,644
189,940
3,489
43,336
132,159
61,439*
95,830
42858


CT-CRMI16A
24,886
43,309
42,582
89,228
23,210
39,408*
26,663
11924


CT-CRMI16A, s68Y
39,555
37,700
6,947
29,050
17,604
22,122*
13,814
6178


CT-CRMV72Y
125,810
75,952
41,286
108,108
210,157
97,834*
63,522
28409


CT-CRMs68Y, V72Y
10,136
27,702
6,289
30,058
1,462
 9,505*
12,950
5792





*Values differ significantly from the saline group













TABLE 16







IgG2b Anti-NTHi rP4 ELISA Endpoint Titers in


Each Individual Mouse on Week 5, Day 6 Sera















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
5,473
132
153
102
66
  237
2,397
1072


CT-CRME29H
85,041
70,413
529
8,597
36,760
15,852 
37,108
16596


CT-CRMI16A
10,554
15,798
7,562
27,484
7,885
12,227*
8,302
3713


CT-CRMI16A, s68Y
13,545
9,942
2,731
8,724
6,257
 7,253*
4,048
1810


CT-CRMV72Y
25,782
23,712
14,433
25,347
74,216
27,798*
23,664
10583


CT-CRMs68Y, V72Y
5,096
14,958
3,205
16,081
582
 4,697*
7,074
3164





*Values differ significantly from the saline group













TABLE 17







IgG3 Anti-NTHi rP4 ELISA Endpoint Titers in


Each Individual Mouse on Week 5, Day 6 Sera















Adjuvant
1
2
3
4
5
GMT
StDev
SEM


















Saline
992
33
33
33
33
65
429
192


CT-CRME29H
311
36
23
344
1,246
162
500
224


CT-CRMI16A
2,256
62
390
290
150
299
918
411


CT-CRMI16A, s68Y
120
1,953
33
612
64
198
816
365


CT-CRMV72Y
479
929
25
1,124
462
357
432
193


CT-CRMs68Y, V72Y
202
69
3
92
33
42
76
34









EXAMPLE 6 
The Immune Responses of BALB/C Mice Immunized with the Purified Native Fusion (F) Glycoprotein of Respiratory Syncytial Virus (RSV)
The capacity of the CT-CRMs of the present invention to augment systemic and mucosal immune responses against respiratory syncytial virus (RSV) glycoproteins was examined using the purified native fusion (F) protein. Previously it was demonstrated that BALB/c mice immunized IN with F protein adjuvanted with either CT or CT-CRME29H generated systemic and local IgG and IgA titers (Tebbey et al, cited above). That study also indicated that pre-existing anti-CT antibodies did not have a negative impact on the level of local or systemic anti-F protein IgA and IgG antibodies. Indeed, the study indicated that pre-existing anti-CT antibodies were beneficial for the generation of an augmented anti-F protein antibody response. Additionally, the data also suggested a mechanism involving the neutralization of infectious virus by either mucosal or humoral immunoglobulins that were stimulated in response to the IN immunization protocol containing F/CT-CRME29H.
BALB/c mice (5 per group) were immunized (IN, 0.01 n-m) at weeks 0 and 3 with native purified F protein (3 μg/dose) alone in saline or in a formulation containing 0.1 or 1 μg of one of the wild-type CT (Sigma) or 0.1 or 1 μg of one of the genetically detoxified mutant CT-CRMs (CT-CRME29H, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y and CT-CRMS68Y,V72Y). Sera were collected 2 weeks post secondary immunization. The titration of the protein-specific IgG, IgA and IgG subclass, serum neutralizing, antibodies in the bronchoalveolar wash, nasal wash and vaginal wash was performed in duplicate on HEp-2 cell monolayers in 96-well microplates. Furthermore, a subgroup of immunized mice was challenged with live virus to determine the protective capacity of the immunogenic formulations. The results are presented in Table 20. The numbers are geometric mean endpoint anti-F protein IgG, subclass and IgA antibody titers (±1 standard deviation).
Analysis of serum antibodies post-secondary immunization showed that immunization with any of the cholera toxin-derived adjuvants significantly induced immune responses to RSV F protein (Table 18). The use of each of the cholera toxin mutants CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y, CT-CRMS68Y,V72Y and CT-CRME29H, at concentrations of 0.1 or 1.0 μg/dose significantly (p<0.05) induced serum antibodies (total IgG, IgG1, IgG2a and IgA) to RSV F protein. The magnitude of the total IgG immune response to RSV F protein was increased approximately 25-fold by inclusion of the cholera toxin-derived adjuvants when compared to the response achieved by animals administered a composition containing F/PBS.
Statistical significance of the data reported in Table 18 below is as follows. For total IgG: p<0.05: F/PBS vs. All. p>0.05: F/CT vs. F/CT-CRME29H vs. F/CT-CRMI16A vs. F/CT-CRMI16A,S68Y vs. F/CT-CRMS68Y,V72Y vs. FCT-CRMS68Y,V72Y (at both 0.1 and 1.0 μg/dose). For IgG1: p<0.05: F/PBS vs. All. p>0.05: F/CT vs. F/CT-CRME29H vs. F/CT-CRMI16A vs. F/CT-CRMI16A,S68Y vs. F/CT-CRMV72Y vs. F/CT-CRMS68Y,V72Y (at both 0.1 and 1.0 μg/dose). For IgG2a: p<0.05: F/PBS vs. All. F/CT (0.1 μg) vs. F/CT-CRMI16A (0.1 μg). p>0.05: At 1.0 μg dose, F/CT vs. F/CT-CRME29H vs. F/CT-CRMI16A vs. F/CT-CRMI16A,S68Y vs. F/CT-CRMV72Y vs. F/CT-CRMS68Y,V72Y. At 0.1 μg dose, F/CT vs. F/CT-CRME29H vs. F/CT-CRMI16A,S68Y vs. F/CT-CRMV72Y vs. F/CT-CRMS68Y,V72Y.
No significant differences were observed in total anti-F IgG or IgG1 titers between each of the new mutant toxins (CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y, and CT-CRMS68Y,V72Y) and either CTE29H or wild-type CT. Evidence of statistical differences was observed between specific groups upon analysis of IgG2a and IgA titers. However, these comparisons did not reveal any consistent trends regarding the immunological performance of one mutant versus another.







TABLE 18







The Humoral Immune Responses of BALB/c Mice after Intranasal


Immunization with F Protein and Mutant CT-CRMs












Antigen
Adjuvant (μg)
IgG
IgG1
IgG2a
IgA





F protein
None
4.1 ± 0.6
3.1 ± 0.4
1.9 ± 0.3
1.9 ± 0.4


F protein
CT-CRME29H(1)
6.3 ± 0.7
5.8 ± 0.8
4.9 ± 0.7
4.3 ± 0.6


F protein
CT-CRME29H(0.1)
5.6 ± 0.2
5.4 ± 0.5
4.4 ± 0.2
4.0 ± 0.2


F protein
CT-CRMI16A(1)
5.6 ± 1.6
5.2 ± 1.4
4.7 ± 1.1
4.3 ± 0.2


F protein
CT-CRMI16A(0.1)
5.5 ± 0.3
5.0 ± 0.1
3.9 ± 0.4
3.5 ± 0.5


F protein
CT-CRMV72Y(1)
6.4 ± 0.2
5.8 ± 0.3
5.3 ± 0.3
4.5 ± 0.3


F protein
CT-CRMV72Y(0.1)
5.6 ± 0.2
5.2 ± 0.4
4.1 ± 0.4
3.5 ± 0.2


F protein
CT-CRMI16A, V72Y(1)
6.4 ± 0.1
5.7 ± 0.2
5.3 ± 0.3
4.6 ± 0.4


F protein
CT-CRMI16A, V72Y(0.1)
 5.5 ± 0.04
5.5 ± 0.3
4.8 ± 0.2
4.1 ± 0.3


F protein
CT-CRMS68Y, V72Y(1)
6.5 ± 0.1
5.6 ± 0.2
4.9 ± 0.3
4.8 ± 0.2


F protein
CT-CRMS684, V72Y(0.1)
5.8 ± 0.3
5.6 ± 0.2
4.2 ± 0.3
4.6 ± 0.2


F Protein
CT(1)
6.7 ± 0.3
6.0 ± 0.3
5.7 ± 0.2
5.3 ± 0.5


F protein
CT(0.1)
5.8 ± 0.1
5.7 ± 0.2
5.0 ± 0.4
4.9 ± 0.2









In another experiment, groups of 5 BALB/c mice were immunized (IN, 0.01 ml) at weeks 0 and 3 with native F protein (3 μg/dose). The F protein was admixed with 1 or 0.1 μg of genetically detoxified mutants or wild-type CT. Anti-F protein antibody responses were also analyzed in pooled mucosal wash samples of bronchoalveolar lavage (BAL), nasal wash (NW) and vaginal wash (VW), collected 2 weeks post-secondary immunization (Table 19). The data represent endpoint anti-F protein IgG and IgA antibody titers of pooled samples. As expected, no induction of antibody in mucosal washes from F/PBS immunized mice was observed. However, the potent mucosal adjuvant capacity of each mutant cholera holotoxin was readily apparent. Although no statistical analyses were performed on these pooled samples, some trends surfaced. For example, mice that received F/CT-CRMV72Y (1.0 μg) displayed elevated IgG and IgA in each of the BAL, NW and VW samples taken. In comparison, mutant toxins CT-CRMI16A, CT-CRMI16A,S68Y and CT-CRMS68Y,V72Y appeared to be comparable to CT-CRME29H in adjuvanting local immune responses to RSV F protein.







TABLE 19







The Mucosal Immune Responses of BALB/c Mice after Intranasal Immunization


with F Protein and Genetically Detoxified Mutants











BAL
NW
VW














Antigen
Adjuvant (μg)
IgG
IgA
IgG
IgA
IgG
IgA

















F protein
None
<25
<25
<25
<25
<25
<25


F protein
CT-CRME29H(1)
1569
211
320
793
265
1629


F protein
CT-CRME29H(0.1)
549
<25
45
136
99
202


F protein
CT-CRMI16A(1)
1349
43
415
287
325
1427


F protein
CT-CRMI16A(0.1)
376
<25
103
187
217
350


F protein
CT-CRMV72Y(1)
1177
121
314
280
222
2289


F protein
CT-CRMV72Y(0.1)
144
<25
51
71
48
311


F protein
CT-CRMI16A, V72Y(1)
2093
458
392
627
739
9683


F protein
CT-CRMI16A, V72Y(0.1)
499
39
133
785
134
500


F protein
CT-CRMS68Y, V72Y(1)
1248
79
1181
510
204
1374


F protein
CT-CRMS68Y, V72Y(0.1)
522
25
109
98
81
770


F protein
CT(1)
3271
142
1593
710
619
4136


F protein
CT(0.1)
6436
1037
395
362
1185
1100









In another experiment, BALB/c mice were immunized (IN, 0.01 μl) at weeks 0 and 3 with native F protein (3 μg/dose). The F protein was admixed with 1 or 0.1 μg of each genetically detoxified mutant or wild-type CT. At week 5, mice were challenged with the A2 strain of RSV and lungs harvested 4 days later to quantitate virus infectivity. Each of the mutant cholera holotoxins induced a protective immune response to RSV challenge as measured by viral lung load (Table 20). Data are presented as the mean virus recovered (log10)/g tissue. Neutralizing antibodies were assayed in the presence of 5% guinea pig serum as a source of complement (C′) in bleeds taken two weeks post-secondary immunization. Data show the mean titer (log10) which showed a 60% reduction in pfu/well compared to control wells.
The statistical analyses of the data from the virus infectivity assays is reported as p<0.05: F/PBS vs. all; p>0.05: F/CT-E29H vs. F/CT vs. F/CRM/I16A vs. F/CT-CRMV27Y vs. F/CT-CRMI16A,V72Y vs. F/CT-CRMS68y,V72Y at both 0.1 and 1.0 μg/dose. Serum neutralizing responses: p<0.05: F/PBS vs. all except F/CT-CRMI16A,S68Y (0.1 μg). F/CT(1.0) vs. F/CT-CRMI16A (0.1). F/CT-CRMI16A,S68Y (0.1) vs. F/CT-CRMI16A,S68Y (1.0) F/CT (9.0), F/CT-CRMI16A (1.0), F/CT-CRME29H (1.0), F/CT-CRMV72Y (0.1).
Lungs from mice immunized with F/PBS were clearly populated with RSV (log103.4 pfu/g tissue). In contrast, those mice immunized with F protein co-formulated with mutant cholera holotoxins displayed no detectable virus. A somewhat similar pattern was observed for serum neutralizing antibodies (Table 20). Those mice immunized with F/PBS displayed complement-assisted neutralizing antibodies that were significantly reduced compared to all mice that had received mutant cholera holotoxins as an adjuvant except F/CT-CRMI16A,S68Y at 0.1 μg per dose. Whereas evidence of neutralizing activity was observed in the absence of complement, no statistical differences were observed (Table 20). Collectively these data suggest that the inclusion of the mutant cholera holotoxins CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y, and CT-CRMS68Y,V72Y contributes substantially to the magnitude of the functional immune responses to RSV F protein.







TABLE 20







Functional Immune Responses Elicited by Immunization


with Purified F Protein and Mutant CT-CRMs










GMT pfu/g
Geometric Mean Serum



(Log10)
(Log10)











Antigen
Adjuvant (μg)
Lung Tissue
+C′
−C′





F protein
None
3.38 ± 0.72
1.1 ± 0.2
<1.3


F protein
CT-CRME29H(1)
<1.5 ± 0.03
3.0 ± 0.6
1.6 ± 0.6


F protein
CT-CRME29H(0.1)
<1.5 ± 0.1 
2.7 ± 0.5
<1.3


F protein
CT-CRMI16A(1)
<1.6 ± 0.03
3.1 ± 0.3
1.5 ± 0.7


F protein
CT-CRMI16A(0.1)
<1.5 ± 0.03
2.3 ± 0.7
<1.3


F protein
CT-CRMV72Y(1)
<1.6 ± 0.05
2.9 ± 0.5
1.6 ± 0.6


F protein
CT-CRMV72Y(0.1)
<1.5 ± 0.03
1.8 ± 0.4
1.1 ± 0.1


F protein
CT-CRMI16A, V72Y(1)
<1.5 ± 0.02
2.7 ± 0.7
1.4 ± 0.6


F protein
CT-CRMI16A, V72Y(0.1)
<1.5 ± 0.05
3.1 ± 0.2
1.5 ± 0.6


F protein
CT-CRMS68Y, V72Y(1)
<1.6 ± 0.05
2.5 ± 0.6
1.4 ± 0.6


F protein
CT-CRMS684, V72Y(0.1)
<1.5 ± 0.5 
2.5 ± 0.5
<1.3


F protein
CT(1)
<1.5 ± .07 
3.5 ± 0.4
1.4 ± 0.6


F protein
CT(0.1)
<1.6 ± 0.06
2.6 ± 0.5
1.5 ± 0.6









In yet additional experiments, naive BALB/c mice (8-10 weeks of age, 5/group) were immunized (IN, 10 1) at weeks 0 and 3 with native purified fusion (F) protein purified from the 248/404 strain of RSV. The protein (3 μg/dose) was prepared in mixture with 1.0 or 0.1 μg of the indicated CT-CRM. Control mice were immunized with F protein admixed with CT-CRME29H alone, with wild-type CT, or with PBS. Serum (geometric mean titer ±1 standard deviation) and bronchoalveolar (BAW), nasal (NW) and vaginal (VW) wash fluids were collected two weeks after secondary immunization for the determination of end-point anti-F protein total and subclass IgG and IgA titers by ELISA. The mucosal wash samples were pooled for the determination of endpoint titers.
The results from two experiments are presented in Tables 21 and 22.







TABLE 21







Geometric Serum Ig Titers of BALB/c Mice Immunized


with F Protein Formulated with the Mutant CT-CRMs









Anti-F Protein Ig Titers (Log10)












Antigen
Adjuvant (μg)
IgG
IgG1
IgG2a
IgA





F protein
None
4.0 ± 0.7
2.7 ± 1.0
2.3 ± 0.7
2.3 ± 0.7


F protein
CT-CRMI16A(1)
5.4 ± 0.3
5.1 ± 0.2
5.1 ± 0.3
4.3 ± 0.3


F protein
CT-CRMI16A(0.1)
5.1 ± 0.4
4.6 ± 0.4
4.0 ± 0.5
3.9 ± 0.5


F protein
CT-CRMI16A, S68Y(1)
5.7 ± 0.2
5.4 ± 0.3
5.3 ± 0.3
4.7 ± 0.3


F protein
CT-CRMI16A, S68Y(0.1)
5.2 ± 0.3
5.5 ± 0.3
4.2 ± 0.2
4.0 ± 0.2


F protein
CT-CRMV72Y(1)
5.6 ± 0.1
5.4 ± 0.2
5.0 ± 0.2
4.7 ± 0.1


F protein
CT-CRMV72Y(0.1)
5.3 ± 0.3
4.8 ± 0.2
4.7 ± 0.2
4.6 ± 0.2


F protein
CT-CRMS68Y, V72Y(1)
5.7 ± 0.2
5.5 ± 0.3
4.3 ± 0.3
4.4 ± 0.2


F protein
CT-CRMS68Y, V72Y(0.1)
5.1 ± 0.2
4.5 ± 0.4
4.0 ± 0.3
4.1 ± 0.3


F protein
CT-CRME29H(1)
5.4 ± 0.3
5.4 ± 0.1
5.5 ± 0.6
4.6 ± 0.3


F protein
CT-CRME29H(0.1)
5.3 ± 0.4
5.4 ± 0.1
4.3 ± 0.4
4.3 ± 0.1


F protein
CT(1)
5.4 ± 0.5
5.0 ± 0.5
4.4 ± 0.8
4.6 ± 1.0


F protein
CT(0.1)
4.6 ± 0.3
4.5 ± 0.4
3.5 ± 0.2
4.3 ± 0.3
















TABLE 22







The Ig Titers of Pooled Mucosal Wash Samples from BALB/c Mice


Immunized with F Protein Formulated with Mutant CT-CRMs









Anti-F Protein Ig Titers











BAW
NW
VW














Antigen
Adjuvant (μg)
IgG
IgA
IgG
IgA
IgG
IgA

















F protein
None
<25
<25
<25
157
<25
44


F protein
CT-CRMI16A(1)
1,177
60
1,062
1,319
270
1,773


F protein
CT-CRMI16A(0.1)
340
75
280
228
57
8,008


F protein
CT-CRMI16A, S68Y(1)
6,029
917
656
1,543
1,200
7,660


F protein
CT-CRMI16A, S68Y(0.1)
2,318
1,028
273
415
669
5,904


F protein
CT-CRMV72Y(1)
5,879
497
6,327
1,940
1,325
4,632


F protein
CT-CRMV72Y(0.1)
4,696
2,954
764
1,002
1,726
684


F protein
CT-CRMS68Y, V72Y(1)
2,179
364
444
4,153
907
849


F protein
CT-CRMS68Y, V72Y(0.1)
1,030
125
289
646
440
201


F protein
CT-CRME29H(1)
1,972
217
616
437
327
43,466


F protein
CT-CRME29H(0.1)
1,893
222
1,993
1,013
845
5,489


F protein
CT(1)
2,189
434
269
474
1,308
994


F protein
CT(0.1)
1,791
308
316
1,997
315
358









When the CT-CRM mutants of this invention were used as mucosal adjuvants at the 1.0 μg dose, results similar to the use of mutant CT-CRME29H or wild-type CT were obtained (Table 21). Noteworthy differences from the anti-F protein IgG or IgA titers elicited following immunization with F protein admixed with CT-CRME29H or wild-type CT were not observed. Because pooled samples were used to determine Ig titers in mucosal wash fluids, statistical analyses could not be performed. Nonetheless, the titers elicited by the mutant CT-CRMs of this invention were comparable to those induced by F protein admixed with CT-CRME29H or wild-type CT (Table 22).
Thus, all CT-CRM mutants of this invention had adjuvant activity for F protein.
EXAMPLE 7 
The Immune Responses of BALB/C Mice Immunized with the USPA2 Outer Membrane Protein of M. Catarrhalis 
In this study, the capacity of mutant CT-CRMs to augment systemic and mucosal immune responses against the native UspA2 outer membrane protein of M. catarrhalis was examined. Purified UspA2 (5 μg/dose) alone in 10 μl saline or in a 10 μl formulation containing 0.1 μg/dose of a mutant CT-CRM (CT-CRME29H, CT-CRMI16A, CT-CRMI16A,S68Y, CT-CRMV72Y or CT-CRMS68Y, V72Y) was administered to Balb/c mice IN on days 0, 7 and 14. Protein-specific IgG and IgA levels in the serum and in mucosal lavages were examined on day 28. The resulting serum and mucosal IgG titers are shown in Table 23. All mutant CT-CRMs, except CT-CRMI16A, elicited enhanced serum IgG antibody response. The levels of IgG and IgA in bronchial, nasal and vaginal washes were measured. No IgA was detected in any of the washes, and IgG was detected only in a few washes.







TABLE 23







Titers of sera to UspA2 elicited in mice by UspA2


administered intranasally with different CT-CRMs.










Serum Antibodies
Mucosal IgG antibodies












CT Mutants
IgG (log10titer ISD)
IgA
Lung
Nose
Vagina















None
 530 (2.724 ± 0.38)
<10
<10
<10
<10


CT-CRME29H
17,378 (4.24 ± 0.47) 
37
35
<10
<10


CT-CRMI16A
 548 (2.739 ± 0.48)
<10
<10
<10
<10


CT-CRMI16A, S68Y
7943 (3.90 ± 1.15)
45
23
<10
15


CT-CRMV72Y
9550 (3.98 ± 0.82)
42
45
<10
19


CT-CRMS68Y, V72Y
1072 (3.03 ± 0.89)
<10
<10
<10
<10









EXAMPLE 8 
Adjuvanticity of Mutant Cholera Toxin Holotoxins
To create a comprehensive panel of mutant CT-CRMs with different characteristics of toxicity, functionality and immunogenicity, the above-described CT-CRM mutants were analyzed as mucosal adjuvants, and the toxicity and enzymatic activity profiles of each of the mutants were determined. As summarized in Table 24, all of the mutant CT-CRMs have significantly reduced toxicity and enzyme activity compared to wild-type CT. The following data was generated from two studies performed to evaluate these genetically detoxified mutant CTs for their capacity to adjuvant immune responses to native UspA2 protein from M. catarrhalis. 
The experiments were performed as follows: BALB/c mice (6-8 weeks old, 5 mice/group) were immunized at weeks 0, 2 and 4 with 5 μg of purified native UspA2 protein in PBS or co-formulated with 1 μg of wild-type CT, or CT-CRME29H, or CT-CRMI16A, or CT-CRMV72Y, or CT-CRMI16A,S68Y, or CT-CRMS68Y,V72Y per immunization. A total volume of 10 μl was administered intranasally (5 μl per nostril). Mice were bled at weeks 0, 2, 4, or 6 in order to assay serum antibody responses. Two weeks after the last immunization (week 6), mice were sacrificed for the analysis of mucosal antibody responses. Significant differences between groups were determined by the Tukey-Kramer HSD multiple comparisons test using JMP® statistical discovery software (SAS Institute Inc., Cay, N.C.).
Adjuvanticity of the CT-CRMs can be summarized as follows. Analysis of serum antibodies at week 6 showed that immunization with UspA2 protein formulated with any of the CT-CRM mutants, at a concentration of 1 μg/dose, significantly induced IgG antibody responses to UspA2 protein The magnitude of the total IgG antibody response to UspA2 protein was increased approximately 17-38 fold by inclusion of the CT-derived mutants (excluding CT-CRMI16A,S68Y) (Table 25). No significant differences were observed in total anti-UspA2 IgG titers between the mutant toxins, CT-CRMI16A, CT-CRMV72Y, and CT-CRMS68Y,V72Y and CT-CRME29H, even though they all elicited significantly higher IgG titers than UspA2 protein alone by Tukey-Kramer HSD test (Table 25). The use of each of the CT-CRM mutants also enhanced serum IgG subclass antibodies (IgG1, IgG2a and IgG2b) to UspA2 protein (Table 27). The ratio of IgG1 and IgG2a or IgG2b titers was approximately 1.0, indicating a balanced Th1/Th2 type of immune response.
Anti-UspA2 protein antibody responses were also analyzed in pooled mucosal wash samples (Table 27). As expected, no induction of antibody in bronchoalveolar lavage (BAL), nasal washes (NW), vaginal washes (VW) or saliva from UspA2/PBS immunized mice was observed. However, the potent mucosal adjuvant capacity of CT-CRMI16A, CT-CRMV72Y, CT-CRMS68Y,V72Y, and CT-CRMI16A,S68Y was clearly shown. There were UspA2 specific mucosal IgA antibodies detected in most of the mucosal samples. Although no statistical analysis can be performed on these pooled samples, some trends appeared. For example, mice that received CT-CRMV72Y displayed elevated UspA2 specific IgA antibodies in each of the NW, VW and saliva samples tested.
CT-CRMI16A, CT-CRMV72Y, and CT-CRMS68Y,V72Y are potent mucosal adjuvants for M, catarrhalis UspA2 protein. The serum antibody data showed that all the CT-CRMs except CT-CRMI16A,S68Y at 1 μg dose are equally as capable in adjuvanting immune responses to UspA2 protein as is CT-CRME29H (Tables 25 and 26). The mucosal wash data appears to suggest that all the mutant CT-CRMs retain potent mucosal adjuvant properties (Table 27). Furthermore, they all have significantly lower residual toxicity and enzyme activity than wild-type CT, as shown in Table 24. Therefore, CT-CRMI16A, CT-CRMV72Y, CT-CRMS68Y,V72Y and CT-CRMI16A,S68Y are additional effective mucosal adjuvants.







TABLE 24







Characterization of Mutant Cholera Toxins















ADP-





Y-1 cell
Ribosyl-



Homogeneity
Holotoxin
toxicity
transferase


Mutant CT
(%)
(%)
(%)
activity (%)














CT-CRMI16A
>90
Not done
0.37
3.3


CT-CRMI16A, S68Y
75.8
97.8
0.37
2.4


CT-CRMV72Y
95.5
99.4
0.37
1.1


CT-CRMS68Y, V72Y
78.9
Not done
0.37
1.2









Groups of five female BALB/c mice were immunized intranasally at weeks 0, 2, and 4 with 10 μL containing 5 μg nUspA2 adjuvanted with 1 μg CT (Sigma) or CT mutants. Endpoint antibody titers were determined from sera collected at week 5 day 6. Data are presented as the geometric mean (±1 SD) of the reciprocal dilution resulting in an OD405 of 0.1. Statistical analysis was by Tukey-Kramer. The results are shown in Table 25.







TABLE 25







The serum anti-nUspA2 responses of BALB/c mice after intranasal


immunization with nUspA2 adjuvanted with mutant CTs













Mean log 10 Antibody Titers



Antigen

(±1 SD)











Group
(5 μg)
Adjuvant (1 μg)
IgG
IgA





AG414
nUspA2
PBS
<2.00
<2.00


AH415
nUspA2
CT
4.08 ± 0.20*
2.47 ± 0.33


AH416
nUspA2
CT-CRME29H
3.37 ± 0.37*
2.04 ± 0.09


AH417
nUspA2
CT-CRMI16A
3.23 ± 0.21*
2.00 ± 0.02


AH418
nUspA2
CT-CRMI16A, S68Y
 2.63 ± 0.12*Φ
<2.00


AH419
nUspA2
CT-CRMV72Y
3.59 ± 0.27*
2.11 ± 0.15


AH420
nUspA2
CT-CRMS68Y, V72Y
3.41 ± 0.22*
<2.00





*Significantly higher than the nUspA2/PBS group


ΦSignificantly lower than all other adjuvanted groups






Groups of five female BALB/c mice were immunized intranasally at weeks 0, 2, and 4 with 10 μL containing 5 μg nUspA2 adjuvanted with 1 μg CT (Sigma) or CT mutants. Endpoint antibody titers were determined from sera collected at week 5 day 6. Data are presented as the geometric mean (±1 SD) of the reciprocal dilution resulting in an OD405 of 0.1. Statistical analysis was by Tukey-Kramer. The results are shown in Table 26.







TABLE 26







The serum anti-nUspA2 responses of BALB/c mice after intranasal


immunization with nUspA2 adjuvanted with mutant CT-CRMs











Antigen
Adjuvant
Mean log 10 Antibody Titers (±1 SD)












Group
(5 μg)
(1 μg)
IgG1
IgG2a
IgG2b





AG414
nUspA2
PBS
<2.00
<2.00
<2.00


AH415
nUspA2
CT
3.27 ± 0.12*
3.39 ± 0.34*
3.03 ± 0.17*


AH416
nUspA2
CT-CRME29H
2.60 ± 0.08*
2.82 ± 0.32*
2.68 ± 0.25*


AH417
nUspA2
CT-CRMI16A
2.30 ± 0.20 
2.91 ± 0.24*
2.54 ± 0.21*


AH418
nUspA2
CT-CRMI16A, S68Y
2.36 ± 0.09*
2.65 ± 0.21*
2.44 ± 0.24*


AH419
nUspA2
CT-CRMV72Y
3.07 ± 0.28*
3.30 ± 0.35*
2.83 ± 0.19*


AH420
nUspA2
CT-CRMS68Y, V72Y
2.80 ± 0.25*
2.86 ± 0.43*
2.52 ± 0.18*





*Significantly higher than the nUspA2/PBS group






Groups of five female BALB/c mice were immunized intranasally at weeks 0, 2, and 4 with 10 μL containing 5 μg nUspA2 adjuvanted with 1 μg CT (Sigma) or CT mutants. Endpoint antibody titers were determined from pooled mucosal wash samples collected at week 6. Data are presented as the reciprocal dilution resulting in an OD405 of 0.1. The results are shown in Table 25.







TABLE 27







The mucosal anti-nUspA2 responses of BALB/c mice after intranasal


immunization with nUspA2 adjuvanted with mutant CTs
















Branch
Nasal
Vaginal




Antigen
Adjuvant
Wash
Wash
Wash
Saliva

















Group
(5 μg)
(1 μg)
IgG
IgA
IgG
IgA
IgG
IgA
IgG
IgA




















AG414
nUspA2
PBS
<10
<10
<10
<10
<10
<10
<10
<10


AG415
nUspA2
CT
<10
<10
<10
34
<10
35
<10
33


AG416
nUspA2
CT-CRME29H
<10
<10
<10
22
<10
<10
<10
<10


AG417
nUspA2
CT-CRMI16A
<10
<10
<10
<10
<10
17
<10
22


AG418
nUspA2
CT-CRMI16A, S68Y
<10
<10
<10
23
<10
12
<10
17


AG419
nUspA2
CT-CRMV72Y
<10
16
<10
15
<10
58
<10
46


AG420
nUspA2
CT-CRMS68Y, V72Y
<10
<10
<10
14
<10
41
<10
43









All publications and references cited in this specification are incorporated herein by reference. While the invention has been described with reference to a particularly preferred embodiment, it will be appreciated that modifications can be made without departing from the spirit of the invention. Such modifications are intended to fall within the scope of the appended claims.

## Claims

1. An immunogenic, mutant cholera holotoxin (CT-CRM) comprising an amino acid sequence of subunit A of the wild-type cholera toxin (CT), wherein said subunit A comprises at least an amino acid substitution in the wild-type CT subunit A amino acid position 16, and wherein said mutant CT-CRM has reduced toxicity compared to said wild-type CT.

2. The CT-CRM according to claim 1, comprising a single amino acid substitution wherein the amino acid isoleucine in the amino acid position 16 in the A subunit is substituted with an alanine.

3. An immunogenic composition comprising a mutant cholera holotoxin (CT-CRM) of claim 1, wherein the mutant holotoxin enhances the immune response in a vertebrate host to an antigen.

4. The composition according to claim 3, further comprising a diluent, excipient or carrier.