# Patent

## Title
Coccidial vaccine and methods of making and using same

## Abstract

The present invention relates to a vaccine for coccidiosis in chickens prepared from three attenuated Eimeria species: E. acervulina, E. maxima and E. tenella. The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.

## Background
RELATED APPLICATIONS
This application is a continuation-in-part of pending U.S. application Ser. No. 11/106,780, filed Apr. 15, 2005, which issued as U.S. Pat. No. 6,998,127 on Feb. 14, 2006, which is a continuation of U.S. application Ser. No. 10/730,206, filed Dec. 8, 2003, which issued as U.S. Pat. No. 6,908,620 on Jun. 21, 2005, which claims priority to U.S. Provisional Application Ser. No. 60/432,298 entitled: “COCCIDIAL VACCINE AND METHODS OF MAKING AND USING THE SAME”, filed Dec. 9, 2002, the disclosure of which is incorporated by reference in its entirety.
The foregoing applications, and all documents cited therein or during their prosecution (“appln cited documents”) and all documents cited or referenced in the appln cited documents, and all documents cited or referenced herein (“herein cited documents”), and all documents cited or referenced in herein cited documents, together with any manufacturer's instructions, descriptions, product specifications, and product sheets for any products mentioned herein or in any document incorporated by reference herein, are hereby incorporated herein by reference, and may be employed in the practice of the invention.


FIELD OF THE INVENTION
The present invention relates to the preparation of immunogenic compositions and vaccines against diseases caused by coccidia. The present invention also provides for attenuated vaccines against coccidiosis.
BACKGROUND OF THE INVENTION
Coccidiosis is a disease caused by infection with one or more of the many species of coccidia which is a subdivision of the phylum Protozoa, intracellular protozoal parasites of the subphylum Apicomplexa and the genus Eimeria. The genus Eimeria contains the species of major economic importance in domestic birds, such as chickens, ducks, geese, guinea fowl, peafowl, pheasants, pigeons and turkeys. While coccidiosis occurs in practically all kinds of birds, the parasites are host specific and each species occurs in a single or in a limited group of related hosts. On the other hand, avian hosts are known to harbor more than one species of coccidia. Species of Eimeria that cause coccidiosis in chickens include E. acervulina, E. brunetti, E. hagani, E. maxima, E. mitis, E. mivati, E. necatrix, E. praecox and E. tenella. E. acervulina is one of the most common species found in the litter of broiler houses. It has a great reproductive potential and is regarded as pathogenic because it produces a marked depression in gain of body weight, higher feed conversion and it produces gross lesions in the upper small intestine.
Among domesticated birds, chickens are the most susceptible to significant economic losses from coccidiosis, although losses can also occur within turkeys, geese, ducks, and guinea fowl. Coccidiosis has also produced serious losses in pheasants and quail raised in captivity. The effects of a coccidiosis infection can take the highly visible form of devastating flock mortality, but another undesirable effect is morbidity and/or weight loss which results from infection.
During the life cycle, the Eimeria parasite passes through a number of stages (see, e.g., U.S. Pat. No. 6,100,241 for an overview). The life cycle begins when the chicken ingests the infectious stage, known as the sporulated oocyst, during ground feeding or by inhalation of dust. The wall of the sporulated oocyst is ruptured by a combination of mechanical grinding action and chemical action in the gizzard and intestinal tract, resulting in the release of four sporocysts. The sporocysts pass into the duodenum where they are exposed to bile and digestive enzymes resulting in the release of two sporozites per sporocyst.
The sporozoites are mobile and search for suitable host epithelium cells in order to penetrate and reproduce in them. Following infection of an epithelium cell, the parasite enters the schizont phase of its life cycle, producing from 8 to 16 to >200 merozoites per schizont. Once released from the schizont, the merozoites are free to infect further epithelium cells. After two to five of these asexual reproduction cycles, the intracellular merozoites grow into sexual forms known as the female or macrogametocyte and the male or microgametocyte. Following fertilization of the macrogametocyte by the microgametes released from the microgametocyte, a zygote is formed which creates a cyst wall about itself. The newly formed oocyst is passed out of the infected chicken with the fecal droppings.
With the correct environmental conditions of temperature and humidity and sufficient oxygen in the air, the oocyst will sporulate into the infectious stage, ready to infect a new host and thereby spreading the disease. Thus, no intermediate host is required for transfer of the parasite from bird to bird.
The result of the Eimeria parasite infecting the digestive tract of a chicken may be a reduction in weight gain, increased feed conversion, cessation of egg production and, in some cases, death. The increase in intensive production of poultry has been accompanied by severe losses due to this parasite; indeed, coccidiosis has become an economically important parasitic disease.
In the past, several methods have been used in attempts to control coccidiosis. Prior to the advent of chemotherapeutic agents, improved sanitation using disinfectants, together with the mechanical removal of litter, was the main method employed; sufficient oocysts, however, usually remained to transmit the disease. The introduction of coccidiostatic agents in the feed or drinking water, in addition to good management, resulted in some success at disease control. Such agents have been found to suffer from a drop in effectiveness over the years, due partly to the development of drug resistant strains of coccidia. Furthermore, several chemotherapeutic agents have been found to leave residues in the meat, making it unsuitable for consumption.
U.S. Pat. Nos. 4,438,097; 4,639,372; 4,808,404; 5,055,292; 5,068,104; 5,387,414; 5,602,033; 5,614,195; 5,635,181; 5,637,487; 5,674,484; 5,677,438; 5,709,862; 5,780,289; 5,795,741; 5,814,320; 5,843,722; 5,846,527; 5,885,568; 5,932,225; 6,001,363 and 6,100,241 relate to coccidiosis vaccines, including live and recombinant vaccines. However, there are problems with existing coccidiosis vaccines, such as reduced efficacy, cross-infection with other parasites (e.g., Clostridium spp.) and poor bird performance. Thus, there exists a need for efficacious coccidiosis vaccines with reduced or non-existent cross-infection that do not adversely affect bird performance.
Citation or identification of any document in this application is not an admission that such document is available as prior art to the present invention.
SUMMARY OF THE INVENTION
This invention is based, in part, on an attenuated coccidiosis vaccine that is efficacious in the face of virulent challenge, reduced cross-infection with Clostridium spp. and has better bird performance as defined by feed conversion rates when compared to other coccidiosis vaccines.
The invention relates to a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Sporulated oocysts were isolated from a seed culture harvested from one or more chickens seeded with a culture of a precocious strain of E. acervulina, E. maxima or E. tenella, i.e., one or more chickens are seeded with either a precocious strain of E. acervulina, E. maxima or E. tenella resulting in three groups of chickens, each seeded with a different Eimeria strain. The isolated sporulated oocysts were combined to formulate a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella 
In an advantageous embodiment, the chickens are 2 to 8 week old SPF chickens. In another advantageous embodiment, about 100 to about 15,000 oocytes are seeded per chicken to generate the seed culture. In another advantageous embodiment, the sporulated oocysts from the seed culture are isolated by centrifugation.
The present invention also provides for verifying the sporulated oocysts are characteristic of the precocious strain of E. acervulina, E. maxima or E. tenella. 
The invention relates to a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the mixture is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the mixture is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. More advantageously, the mixture is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella are combined.
The invention also relates to specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima E. tenella is about 5:1:1 (i.e., 10:2:2).
The invention also relates to testing the efficacy of the mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Advantageously, the testing relates to administering a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal. In a more advantageous embodiment, the challenge dose is about 200,000 oocysts of E. acervulina and about 20,000 to about 500,000 oocysts of E. maxima, or about 20,000 to about 50,000 oocysts of E. tenella. 
The present invention relates to immunizing a chicken, advantageously a broiler chicken. However, methods of making the vaccine described herein can be extrapolated to other animals infected by Eimeria, in particular avians such as, but not limited to, a chicken, duck, goose, guinea fowl, peafowl, pheasant, pigeon, quail or turkey.
The invention encompasses an immunogenic or vaccine composition comprising a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella. In an advantageous embodiment, the mixture is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the mixture is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. More advantageously, the mixture is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella are combined.
The invention also relates to an immunogenic or vaccine composition comprising specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima: E. tenella is about 5:1:1 (i.e., 10:2:2).
The invention also provides eliciting an immune response or inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition comprising a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella to elicit or induce the response in an animal. Advantageously, the animal is an avian such as, but not limited to, a chicken, duck, goose, guinea fowl, peafowl, pheasant, pigeon, quail or turkey. In the most advantageous embodiment, the avian is a chicken, advantageously a broiler chicken. The method of eliciting an immune response or inducing an immunological response can also include administering an adjuvant, a cytokine or both.
Advantageously, the effective amount to elicit an immune response or induce an immunological or protective response is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the effective amount is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. More advantageously, the effective amount is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella are combined. In another embodiment, the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal. More advantageously, the challenge dose is about 200,000 oocysts of E. acervulina and about 20,000 to about 500,000 oocysts of E. maxima or about 20,000 to about 50,000 oocysts of E. tenella. 
The effective amount to elicit an immune response or induce an immunological or protective response can also be expressed as ratios of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima: E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima:E. tenella is about 5:1:1 (i.e., 10:2:2).
In consideration of the prevalence and pathogenicity of various Eimeria species, a successful attenuated coccidiosis vaccine should contain the least number of Eimeria strains sufficient to elicit an immune response or induce an immunological or protective response that is non-pathogenic to the recipient of the vaccine. The present relation relates to a combination of oocysts from four specific precocious strains of Eimeria, i.e., E. acervulina, E. maxima and E. tenella that results in an efficacious and non-pathogenic vaccine. The addition of other Eimeria strains, such as E. brunetti, E. necatrix and E. praecox may be disadvantageous with respect to efficacy, cross-infection or pathogenicity of the vaccine. Since E. brunetti, E. necatrix and E. praecox are not necessary for the efficacy of the coccidiosis vaccine disclosed herein, it would be advantageous to exclude these strains from the vaccine of the present invention.
It is noted that in this disclosure and particularly in the claims and/or paragraphs, terms such as “comprises”, “comprised”, “comprising” and the like can have the meaning attributed to it in U.S. Patent law; e.g., they can mean “includes”, “included”, “including”, and the like; and that terms such as “consisting essentially of” and “consists essentially of” have the meaning ascribed to them in U.S. Patent law, e.g., they allow for elements not explicitly recited, but exclude elements that are found in the prior art or that affect a basic or novel characteristic of the invention.
These and other embodiments are disclosed or are obvious from and encompassed by, the following Detailed Description.


DETAILED DESCRIPTION
This invention is based, in part, on an attenuated coccidiosis vaccine that is efficacious in the face of virulent challenge, reduced cross-infection with Clostridium spp. and has better bird performance as defined by feed conversion rates when compared to other coccidiosis vaccines.
The invention relates to a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Sporulated oocysts were isolated from a seed culture harvested from one or more chickens seeded with a culture of a precocious strain of E. acervulina, E. maxima or E. tenella, i.e., one or more chickens are seeded with either a precocious strain of E. acervulina, E. maxima or E. tenella resulting in three groups of chickens, each seeded with a different Eimeria strain. Optionally, sporulated oocysts from precocious strains of E. mitis may also be added to the mixture of sporulated oocysts.
Advantageously, the Eimeria strain is a precocious strain. Precocious strains are derived from field species are not pathogenic when administered at the right dose. In an advantageous embodiment, the Eimeria strain is a precocious strain of the respective microorganism as described in Avian Pathology, 17: 305-314, 1988 entitled “Eimeria of American Chickens: Characteristics of Six Attenuated Strains Produced by Selection for Precocious Development, P. L. Long and Joyce K. Johnson, the disclosure of which is incorporated by reference in its entirety. Microorganisms can be attenuated by their selection for precocious development as described as described in Avian Pathology, 17: 305-314, 1988 entitled “Eimeria of American Chickens: Characteristics of Six Attenuated Strains Produced by Selection for Precocious Development, P. L. Long and Joyce K. Johnson, the disclosure of which is incorporated by reference in its entirety. Briefly, the microorganisms are attenuated by selecting for an earlier pre-patent period, i.e., when the oocysts first show up in the feces. Such methods are well known to one of skill in the art and constitute routine experimentation.
Stock cultures of the Eimeria strains for the seed cultures include, but are not limited to, the following. The parent of the Eimeria acervulina, obtained from T. K. Jeffers at Hess and Clark Laboratories in 1969, was thought to have been isolated by Dr. M. M. Farr at USDA, Beltsville, Md., which was derived from a single oocyst. The Eimeria maxima culture was derived from an interbred mixture of 10 purified isolates obtained from Georgia, Delaware, Maryland, Virginia and Texas. The parent of the Eimeria mitis culture was isolated from Gainesville, Ga. in July 1978 and was purified by single oocyst isolation. The parent of the Eimeria tenella culture was obtained from a culture maintained at Pennsylvania State University by Dr. Patten since the early 1960's, and was acquired by the University of Georgia in 1982. Other precocious Eimeria strains include LS100 precocious E. Acervulina isolate 809-13, and LS precocious E. mitis, obtained from Merck Research Laboratories, which were obtained from Dr. Peter Long. Alternatively, attenuated precocious Eimeria lines that have been deposited as sporocysts at the European Collection of Animal Cell Cultures (“ECACC”) as patent deposits (see, e.g., U.S. Pat. No. 5,055,292, the disclosure of which is incorporated by reference in its entirety) are useful stock cultures to generate the Eimeria seed cultures described herein. Specifically, deposits of E. acervulina (deposit no. ECACC 86072203), E. maxima (deposit nos. ECACC 86112011 and 86112012), E. mitis (deposit no. ECACC 86072206) and E. tenella (deposit no. ECACC 86072201) as described in U.S. Pat. No. 5,055,292 are useful stock cultures for the seed cultures of the present invention.
Advantageously, the microorganisms are attenuated by their selection for precocious development as described above. In another advantageous embodiment, the culture is pathogen-free. The stock cultures described above are advantageously maintained in the liquid or vapor phase of liquid nitrogen. Such methods are known to one of skill in the art.
In an advantageous embodiment, the chickens are two to eight weeks old. Sporulated oocysts are passed successively, without limitations to the passage, in chickens until the number of oocysts are sufficient to be used as seed for production. Advantageously, the cultures should not be held for longer than 12 months in order to maintain viability/infectivity.
In an advantageous embodiment, dedicated facilities are maintained for each Eimeria species. Advantageously, a sufficient volume of sporulated oocysts (seed) is mixed with feed or alternatively, is administered orally to provide each chicken with a minimum dose. In an advantageous embodiment, about 5000 to about 15,000 oocytes are seeded per chicken to generate the seed culture.
The sporulated oocysts from the seed culture are isolated from the bird feces, advantageously by centrifugation. In an advantageous embodiment, the harvest is as follows. Droppings are homogenized at an approximate ratio of 10% (w/v) in water. Large particles are removed by passing homogenate through screens. Solids are separated by either centrifugation, screening or by holding at 5±3° C. up to 24 hours. If solids are separated by holding at 5±3° C., they are further concentrated by centrifugation. The supernatant is discarded, and the solids are resuspended in a saturated NaCl (80% w/v) solution in water. The resulting solution is centrifuged. The oocysts are collected (removed) from the top of the liquid, and resuspended in water. Optionally, the remaining liquid is diluted to 20-40% NaCl with water and centrifuged. The pellet is then resuspended in a saturated NaCl solution and re-centrifuged, until no additional oocysts are recovered. The oocysts are washed no more than twice. The oocysts are washed free of salt by repeated, resuspension centrifugation cycles-followed by resuspension in a 0.5% solution of sodium hypochlorite for 10 to 15 minutes. The oocysts are then washed free of the sodium hypochlorite solution by repeated (3×) centrifugation and resuspension steps. The final resuspension is made in a 2.5% aqueous solution of potassium dichromate (K2CrO7). The oocysts were then transferred to sporulation vessels. Sporulation is facilitated by sparging the suspensions with air for a period not to exceed 72 hours at 27±3° C. Following sporulation the oocysts are held at 5±3° C. until the final product is produced.
In another embodiment, oocysts to be used in accord with the present vaccination method can be prepared by any of several methods known to those skilled in the art. Such methods include those described in J. F. Ryley at al., Parasitology 73:311-326, 1976, P. L. Long et al., Folia Veterinaria Latina VI#3, 201-217, 1976, and U.S. Pat. No. 6,627,205, the disclosures of which are incorporated by reference in their entireties. According to one method, commercial broiler chickens, approximately 2 weeks old, are infected with the Eimeria species of interest by oral gavage of an appropriate dose of sporulated oocysts. Well known procedures for collection and purification of oocysts from infected birds are then followed. For most species of Eimeria, feces are collected from infected birds 5-7 days post-infection, blended and filtered to remove debris, then centrifuged at a speed sufficient to pellet the remaining fecal material. The pellet is resuspended in a saturated salt solution, in which the oocysts float and most of the contaminating debris can be removed by centrifugation. The oocyst suspension is then diluted to lower the salt concentration. The oocysts are washed repeatedly to remove the salt and resuspended in potassium dichromate solution (2.5% w/v). The oocyst suspension is incubated at 29 C with shaking (e.g., 140 rpm) for approximately 72 hours to induce sporulation of the oocysts. Alternatively, the oocysts can be treated with sodium hypochlorite and then sporulated. The number of sporulated oocysts/ml is determined by direct count using a hemocytometer or McMaster slide, and the culture is stored under refrigeration until needed.
To prepare sporocysts, the potassium dichromate is removed from the oocyst suspension described above by repeated washing of the oocysts, which involves collection of oocysts by centrifugation and resuspending in deionized or distilled water. When the dichromate has been removed as judged by the lack of yellowish-orange coloration, the oocyst suspension is sterilized. Advantageously, the sterilant is beta propiolactone (BPL). In an advantageous embodiment, 97% BPL is diluted 1:10 with sterile water, then 10-20 mls of BPL is added per liter of sporulated oocysts.
Inn an alternate embodiment, the oocyst suspension is mixed with an equal volume of sodium hypochlorite (bleach) and incubated at room temperature for 15 minutes. The bleach is then removed by repeated washings, and the oocysts are resuspended in physiological saline or deionized water.
Oocysts can be broken to release sporocysts using a variety of known techniques. For example, oocysts can be broken to release sporocysts by mixing the oocysts with glass beads of 1-4 mm diameter and shaking by hand, vortex mixer, or shaking incubator, or using a hand-held homogenizer. Unbroken oocysts and oocysts walls can be separated from the released sporocysts by differential centrifugation in 50% PERCOLL, a colloidal suspension of polyvinyl pyrrolidone coated silica particles (sold by Pharmacia Biotech) or 1 M sucrose as described in Dulski et al., Avian Diseases, 32: 235-239, 1988. The sporocysts can be used in the present vaccination method either mixed with or separated from the unbroken oocysts and oocysts walls. Advantageously, the dose of sporocysts is separated from the oocysts and oocysts walls.
In an advantageous embodiment, the specifications for an acceptable harvest of the seed culture are as follows. First, the ratio of sporulated oocysts to total oocysts was determined. Only harvests meeting or exceeding >40% sporulation are considered acceptable. Second, the size, shape and appearance of each oocyst harvest must be characteristic of the species intended to be produced. For example, parameters to be considered in characterizing the Eimeria species include, but are not limited to, DNA-based technologies, DNA buoyant density, enzyme variation, host and site specificity, immunological specificity, pathogenicity, pre-patent period and sporulation time (see, e.g., Long & Joyner, J Protozool. 1984 November; 31(4): 535-41 and Shirley, Acta Vet Hung. 1997; 45(3): 331-47, the disclosures of which are incorporated by reference).
The isolated sporulated oocysts from the seed cultures described herein are combined to formulate a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Generally, the mixture is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the range of sporulated oocysts in the mixture is about 125 to about 500 oocysts of E. acervulina, about 25 to about 100 oocysts of E. maxima and about 25 to about 500 oocysts of E. tenella. In one embodiment, a low dose is about 125 oocysts of E. acervulina, about 25 oocysts of E. maxima and about 25 oocysts of E. tenella. In another embodiment, a medium dose is about 250 oocysts of E. acervulina, about 50 oocysts of E. maxima and about 50 oocysts of E. tenella. In yet another embodiment, a high dose is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. The mixture optionally may comprise about 10 to about 1000 oocysts of E. mitis, advantageously about 125 to about 500 oocysts of E. mitis, about 125 oocysts of E. mitis in a low dose, about 250 oocysts of E. mitis in a medium dose and about 500 oocysts of E. mitis in a high dose.
The mixture optionally may comprise about 10 to about 1000 oocysts of E. mitis, advantageously about 125 to about 500 oocysts of E. mitis, about 125 oocysts of E. mitis in a low dose, about 250 oocysts of E. mitis in a medium dose and about 500 oocysts of E. mitis in a high dose.
The invention also relates to specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima E. tenella is about 5:1:1 (i.e., 10: 2:2).
In an embodiment containing E. mitis, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 10:1 to 2:10:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima, about 10 sporocysts of E. mitis and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 5:1:5:1 (i.e., 10:2:10:2).
Advantageously, the mixture is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. In a more advantageous embodiment, the mixture is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. The mixture may optionally contain about 500 oocysts of E. mitis, advantageously about 500 oocysts of E. mitis. 
Advantageously, the oocysts are suspended in a preservative consisting of a 0.01M phosphate buffered saline solution containing gentamicin. In another embodiment, the oocysts are suspended in any one of a variety of preservatives or organic acids such as, but not limited to, acetic acid, citric acid, potassium dichromate or propionic acid. For example, but not by limitation, sufficient sterile, 0.01M phosphate buffered saline containing not more than 30 mcg/ml gentamicin, is used to yield 2 ml per bottle for a 2,000 dose presentation, 5 ml per bottle for a 5,000 dose presentation and 10 ml per bottle for a 10,000 dose presentation. Advantageously, the oocysts are stored in sterile, borosilicate glass vials. For example, but not by limitation, the oocysts are aseptically filled into vaccine vials with a semi-automatic or automatic dispenser, stoppers are mechanically or manually inserted and aluminum seals are placed and crimped.
In another embodiment, oocysts are suspended in sterile distilled water containing a suspending agent, for example a polysaccharide suspending agent such as a gum, e.g. xanthan gum or gum acacia, a cellulose derivative, e.g. carboxymethyl cellulose, hydroxypropyl methyl cellulose or microcrystalline cellulose, carageenan, sodium alginate, pectin or starch; a polypeptide suspending agent such as gelatin; a synthetic polymer suspending agent such as polyacrylic acid; or a silicate suspending agent such as magnesium aluminium silicate (see, e.g., U.S. Pat. No. 5,055,292, the disclosure of which is incorporated by reference in its entirety).
The present invention also provides for verifying the sporulated oocysts are characteristic of the precocious strain of E. acervulina, E. maxima or E. tenella. Advantageously, all oocysts are attenuated, in that they are precocious. In another advantageous embodiment, the size, shape and appearance of each oocyst harvest must be characteristic of the species intended to be produced. In yet another advantageous embodiment, the mixture of sporulated oocysts is tested for purity, extraneous pathogens, and/or deaths or severe lesions of the test animals, e.g., chickens. The characteristics of the various Eimeria species are fully set out by Long P. L. and Reid W. M. (1982: A Guide for the Diagnosis of Coccidiosis in Chickens; University of Georgia Research Report 404) and Joyner L. P. (1978: Identification and Diagnosis, Avian Coccidiosis, Poultry Science Symposium No. 13, British Poultry Science Ltd), the disclosures of which are incorporated by reference in their entireties.
The invention also relates to testing the efficacy of the mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Advantageously, the testing relates to administering a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal. In a more advantageous embodiment, the challenge dose is about 200,000 oocysts of E. acervulina and about 20,000 to about 500,000 oocysts of E. maxima or about 20,000 to about 50,000 oocysts of E. tenella. 
In an embodiment comprising E. mitis, a challenge dose of about 100,000 to about 500,000 oocysts of E. mitis, advantageously about 200,000 oocysts of E. mitis may be used.
The invention further provides for determining bird performance as defined by feed conversion rates as a result of administering the the mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella to the birds. Feed conversion efficiency is defined as the as pounds of feed to produce a pound of meat. A common result is about 1.90 or 2.00. One point in feed conversion in common lingo is 0.01, which equals about 0.5% (half of a percent). If the metric system is used, the feed conversion is Kg of feed per Kg of meat, and is still proportional to the above.
The present invention relates to immunizing a chicken, advantageously a broiler chicken. However, methods of making the vaccine described herein can be extrapolated to other animals infected by Eimeria, in particular avians such as, but not limited to, a chicken, duck, goose, guinea fowl, peafowl, pheasant, pigeon, quail or turkey, or in a less advantageous embodiment, a rabbit.
The invention encompasses an immunogenic or vaccine composition comprising a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella. The isolated sporulated oocysts are combined to formulate a composition of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. Generally, the mixture is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the range of sporulated oocysts in the composition is about 125 to about 500 oocysts of E. acervulina, about 25 to about 100 oocysts of E. maxima and about 25 to about 250 oocysts of E. tenella. In one embodiment, a low dose is about 125 oocysts of E. acervulina, about 25 oocysts of E. maxima and about 25 oocysts of E. tenella. In another embodiment, a medium dose is about 250 oocysts of E. acervulina, about 50 oocysts of E. maxima and about 50 oocysts of E. tenella. In yet another embodiment, a high dose is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. 
Advantageously, the composition is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. In a more advantageous embodiment, the composition is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. 
The mixture optionally may comprise about 10 to about 1000 oocysts of E. mitis, advantageously about 125 to about 500 oocysts of E. mitis, about 125 oocysts of E. mitis in a low dose, about 250 oocysts of E. mitis in a medium dose and about 500 oocysts of E. mitis in a high dose.
The invention also relates to specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima E. tenella is about 5:1:1 (i.e., 10: 2:2).
In an embodiment containing E. mitis, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 10:1 to 2:10:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima, about 10 sporocysts of E. mitis and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 5:1:5:1 (i.e., 10: 2:10:2).
The term of “immunogenic composition” covers herein any composition able, once it has been administered to an animal, e.g., avian, to elicit a protective immune response against the parasite or antigen or immunogen or epitope. The term of “vaccine” covers herein any composition able, once it has been administered to the animal, e.g., avian, to induce a protective immune response against the virus, or to efficaciously protect the animal against the parasitic invention.
Immunogenic compositions or vaccines according to the invention can also include the pathogen or immunogen, antigen or epitope of the pathogen and at least one immunogen, antigen or epitope of another pathogen, parasite or virus, i.e., the coccidiosis vaccine is combined with another avian vaccine. Such an immunogen, antigen or epitope may e.g. be of bacterial, or parasitic or viral origin or an inactivated or attenuated form of the pathogen, parasite or virus. The invention also comprehends kits to prepare these combination compositions, as well as methods for making these combination compositions and the use of the components of these combination compositions to prepare the combination compositions. Accordingly, the invention involves a kit for preparing the combination immunogenic or vaccine compositions of the invention; for instance, such a kit that comprises (a) an organism, pathogen or virus or antigen or epitope thereof (advantageously a pathogen as mentioned herein) and (b) an organism, pathogen or virus or immunogen, antigen or epitope thereof (advantageously a virus or immunogen, antigen or epitope thereof, but other pathogens as herein mentioned are also contemplated) that is different than (a), in separate containers, optionally in the same package, and optionally with instructions for admixture and/or administration.
Immunogenic compositions and/or vaccines according to the invention can include Eimeria culture or preparation (e.g., inactivated or attenuated Eimeria, or an immunogen or antigen or epitope thereof), and at least one immunogen, antigen or epitope of another avian pathogen (including without limitation the pathogen in inactivated or attenuated form). For avian multivalent immunogenic compositions and multivalent vaccines, the additional avian pathogen(s), as to which additional avian antigen(s) or immunogen(s) or epitope(s) thereof are included in and/or expressed by the multivalent immunogenic compositions and multivalent vaccines, are viruses, diseases, or pathogens of the Marek's disease virus (MDV) (e.g., serotypes 1 and 2, advantageously 1), Newcastle disease virus (NDV), paramyxoviruses other than Newcastle disease (PMV2 to PMV7), infectious bronchitis virus (IBV), infectious anaemia virus or chicken anemia virus (CAV), infectious laryngotracheitis virus (ILTV), infectious bursal disease virus (IBDV), encephalomyelitis virus or avian encephalomyelitis virus (AEV or avian leukosis virus ALV), virus of hemorragic enteritis of turkeys (HEV), pneumovirosis virus (TRTV), fowl plague virus (avian influenza), chicken hydropericarditis virus, avian reoviruses, coccidiosis, egg drop syndrome (EDS76), fowl pox, inclusion body hepatitis (adenovirus), lymphoproliferative disease of turkeys, reticuloendotheliosis in chickens, reticuloendotheliosis in turkeys, rotavirus enteritis, and turkey rhinotracheitis, Clostridium spp., Escherichia coli, Mycoplasma gallinarum, Mycoplasma gallisepticum, Haemophilus avium, Pasteurella gallinarum, Pasteurella multocida gallicida, and mixtures thereof. Advantageously, for MDV the immunogen is advantageously gB and/or gD, e.g., gB and gD, for NDV the immunogen is advantageously HN and/or F, e.g., HN and F; for IBDV the immunogen advantageously is VP2; for IBV the immunogen is advantageously S (more advantageously S1) and/or M and/or N, e.g., S (or S1) and M and/or N; for CAV the immunogen is advantageously VP1 and/or VP2; for ILTV the immunogen is advantageously gB and/or gD; for AEV the immunogen advantageously is env and/or gag/pro, e.g., env and gag/pro or gag/pro; for HEV the immunogen is advantageously the 100K protein and/or hexon; for TRTV the immunogen is advantageously F and/or G, and for fowl plague the immunogen is advantageously HA and/or N and/or NP, e.g., HA and N and/or NP. Thus, the invention also involves methods for making these compositions, as well as kits therefore.
An immunogenic composition or vaccine according to the invention that also comprises such an additional immunogenic component (additional immunogen, antigen or epitope) has the advantage that it induces an immune response or protection against several infections or maladies or causative agents thereof at the same time. This additional immunogenic component can be an attenuated or inactivated micro-organism, a recombinant construct or sub-units (e.g. proteins, glycoproteins, polypeptides, or epitopes). Epitope determination procedures, such as, generating overlapping peptide libraries (Hemmer et al., Immunology Today, 1998, 19 (4), 163-168), Pepscan (Geysen H. M. et al., Proc. Nat. Acad. Sci. USA, 1984, 81 (13), 3998-4002; Geysen H. M. et al., Proc. Nat. Acad. Sci. USA, 1985, 82 (1), 178-182; Van der Zee R. et al., Eur. J. Immunol., 1989, 19 (1), 43-47; Geysen H. M., Southeast Asian J. Trop. Med. Public Health, 1990, 21 (4), 523-533; Multipin Peptide Synthesis Kits de Chiron) and algorithms (De Groot A. et al., Nature Biotechnology, 1999, 17, 533-561), can be used in the practice of the invention, to determine epitopes of immunogens, antigens, polypeptides, glycoproteins and the like, without undue experimentation. From that information, one can construct nucleic acid molecules encoding such an epitope, and from that knowledge and knowledge in the art, one can construct vectors or constructs, e.g., recombinant viruses or vectors or plasmids that express immunogens, epitopes or antigens; all without undue experimentation.
The pharmaceutically or veterinarily acceptable carriers or vehicles or excipients are well known to the one skilled in the art. For example, a pharmaceutically or veterinarily acceptable carrier or vehicle or excipient can be a 0.9% NaCl (e.g., saline) solution or a phosphate buffer. The pharmaceutically or veterinarily acceptable carrier or vehicle or excipients may be any compound or combination of compounds facilitating the administration of the vector (or protein expressed from an inventive vector in vitro); advantageously, the carrier, vehicle or excipient may facilitate transfection and/or improve preservation of the vector (or protein). Doses and dose volumes are herein discussed in the general description of immunization and vaccination methods, and can also be determined by the skilled artisan from this disclosure read in conjunction with the knowledge in the art, without any undue experimentation.
The immunogenic compositions and vaccines according to the invention may comprise or consist essentially of one or more adjuvants. Suitable adjuvants for use in the practice of the present invention are (1) polymers of acrylic or methacrylic acid, maleic anhydride and alkenyl derivative polymers, (2) immunostimulating sequences (ISS), such as oligodeoxyribonucleotide sequences having one ore more non-methylated CpG units (Klinman et al., Proc. Natl. Acad. Sci., USA, 1996, 93, 2879-2883; WO98/16247), (3) an oil in water emulsion, such as the SPT emulsion described on p 147 of “Vaccine Design, The Subunit and Adjuvant Approach” published by M. Powell, M. Newman, Plenum Press 1995, and the emulsion MF59 described on p 183 of the same work, (4) cation lipids containing a quaternary ammonium salt, (5) cytokines, (6) aluminum hydroxide or aluminum phosphate or (7) other adjuvants discussed in any document cited and incorporated by reference into the instant application, or (8) any combinations or mixtures thereof.
The oil in water emulsion (3), which is especially appropriate for viral vectors, can be based on: light liquid paraffin oil (European pharmacopoeia type), isoprenoid oil such as squalane, squalene, oil resulting from the oligomerization of alkenes, e.g. isobutene or decene, esters of acids or alcohols having a straight-chain alkyl group, such as vegetable oils, ethyl oleate, propylene glycol, di(caprylate/caprate), glycerol tri(caprylate/caprate) and propylene glycol dioleate, or esters of branched, fatty alcohols or acids, especially isostearic acid esters. The oil is used in combination with emulsifiers to form an emulsion. The emulsifiers may be nonionic surfactants, such as: esters of on the one hand sorbitan, mannide (e.g. anhydromannitol oleate), glycerol, polyglycerol or propylene glycol and on the other hand oleic, isostearic, ricinoleic or hydroxystearic acids, said esters being optionally ethoxylated, or polyoxypropylene-polyoxyethylene copolymer blocks, such as Pluronic, e.g., L121.
Among the type (1) adjuvant polymers, preference is given to polymers of crosslinked acrylic or methacrylic acid, especially crosslinked by polyalkenyl ethers of sugars or polyalcohols. These compounds are known under the name carbomer (Pharmeuropa, vol. 8, no. 2, June 1996). One skilled in the art can also refer to U.S. Pat. No. 2,909,462, which provides such acrylic polymers crosslinked by a polyhydroxyl compound having at least three hydroxyl groups, preferably no more than eight such groups, the hydrogen atoms of at least three hydroxyl groups being replaced by unsaturated, aliphatic radicals having at least two carbon atoms. The preferred radicals are those containing 2 to 4 carbon atoms, e.g. vinyls, allyls and other ethylenically unsaturated groups. The unsaturated radicals can also contain other substituents, such as methyl. Products sold under the name Carbopol (BF Goodrich, Ohio, USA) are especially suitable. They are crosslinked by allyl saccharose or by allyl pentaerythritol. Among them, reference is made to Carbopol 974P, 934P and 971P.
As to the maleic anhydride-alkenyl derivative copolymers, preference is given to EMA (Monsanto), which are straight-chain or crosslinked ethylene-maleic anhydride copolymers and they are, for example, crosslinked by divinyl ether. Reference is also made to J. Fields et al., Nature 186: 778-780, Jun. 4, 1960.
With regard to structure, the acrylic or methacrylic acid polymers and EMA are preferably formed by basic units having the following formula:




in which:

    
    
        R1 and R2, which can be the same or different, represent H or CH3 
        x=0 or 1, preferably x=1
        y=1 or 2, with x+y=2.
    
    


For EMA, x=0 and y=2 and for carbomers x=y=1.
These polymers are soluble in water or physiological salt solution (20 g/l NaCl) and the pH can be adjusted to 7.3 to 7.4, e.g., by soda (NaOH), to provide the adjuvant solution in which the expression vector(s) can be incorporated. The polymer concentration in the final vaccine composition can range between 0.01 and 1.5% w/v, advantageously 0.05 to 1% w/v and preferably 0.1 to 0.4% w/v.
The cationic lipids (4) containing a quaternary ammonium salt which are advantageously but not exclusively suitable for plasmids, are preferably those having the following formula:




in which R1 is a saturated or unsaturated straight-chain aliphatic radical having 12 to 18 carbon atoms, R2 is another aliphatic radical containing 2 or 3 carbon atoms and X is an amine or hydroxyl group.

Among these cationic lipids, preference is given to DMRIE (N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy)-1-propane ammonium; WO96/34109), preferably associated with a neutral lipid, preferably DOPE (dioleoyl-phosphatidyl-ethanol amine; Behr J. P., 1994, Bioconjugate Chemistry, 5, 382-389), to form DMRIE-DOPE.
Advantageously, the mixture with the adjuvant is formed extemporaneously and preferably contemporaneously with administration of the preparation or shortly before administration of the preparation; for instance, shortly before or prior to administration, the plasmid-adjuvant mixture is formed, advantageously so as to give enough time prior to administration for the mixture to form a complex, e.g. between about 10 and about 60 minutes prior to administration, such as approximately 30 minutes prior to administration.
When DOPE is present, the DMRIE: DOPE molar ratio is preferably about 95: about 5 to about 5:about 95, more advantageously about 1: about 1, e.g., 1:1.
The DMRIE or DMRIE-DOPE adjuvant:plasmid weight ratio can be between about 50: about 1 and about 1: about 10, such as about 10: about 1 and about 1 :about 5, and preferably about 1: about 1 and about 1: about 2, e.g., 1:1 and 1:2.
The cytokine or cytokines (5) can be in protein form in the immunogenic or vaccine composition, or can be co-expressed in the host with the immunogen or immunogens or epitope(s) thereof. Preference is given to the co-expression of the cytokine or cytokines, either by the same vector as that expressing the immunogen or immunogens or epitope(s) thereof, or by a separate vector therefor.
The invention comprehends preparing such combination compositions; for instance by admixing the active components, advantageously together and with an adjuvant, carrier, cytokine, and/or diluent.
Cytokines that may be used in the present invention include, but are not limited to, granulocyte colony stimulating factor (G-CSF), granulocyte/macrophage colony stimulating factor (GM-CSF), interferon α (IFN α), interferon β (IFN β), interferon γ, (IFN γ), interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4), interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-8 (IL-8), interleukin-9 (IL-9), interleukin-10 (IL-10), interleukin-11 (IL-11), interleukin-12 (IL-12), tumor necrosis factor α (TNF α), tumor necrosis factor β (TNF β), and transforming growth factor β (TGF β). It is understood that cytokines can be co-administered and/or sequentially administered with the immunogenic or vaccine composition of the present invention. Thus, for instance, the vaccine of the instant invention can also contain an exogenous nucleic acid molecule that expresses in vivo a suitable cytokine, e.g., a cytokine matched to this host to be vaccinated or in which an immunological response is to be elicited (for instance, an avian cytokine for preparations to be administered to birds).
The invention also provides eliciting an immune response or inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition comprising a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella to elicit or induce the response in an animal. Advantageously, the animal is an avian such as, but not limited to, a chicken, duck, goose, guinea fowl, peafowl, pheasant, pigeon, quail or turkey. In the most advantageous embodiment, the avian is a chicken, advantageously a broiler chicken. The method of eliciting an immune response or inducing an immunological response can also include administering an adjuvant, a cytokine or both.
The effective amount to elicit an immune response or induce an immunological or protective response is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the effective amount to elicit an immune response or induce an immunological or protective response is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. More advantageously, the effective amount is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. In another embodiment, the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal. More advantageously, the challenge dose is about 200,000 oocysts of E. acervulina and about 20,000 to about 500,000 oocysts of E. maxima or about 20,000 to about 50,000 oocysts of E. tenella. 
The mixture optionally may comprise about 10 to about 1000 oocysts of E. mitis, advantageously about 125 to about 500 oocysts of E. mitis, about 125 oocysts of E. mitis in a low dose, about 250 oocysts of E. mitis in a medium dose and about 500 oocysts of E. mitis in a high dose.
The effective amount to elicit an immune response or induce an immunological or protective response also relates to specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima E. tenella is about 5:1:1 (i.e., 10:2:2).
In an embodiment containing E. mitis, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 10:1 to 2:10:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima, about 10 sporocysts of E. mitis and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 5:1:5:1 (i.e., 10: 2:10:2).
Another aspect of the present invention is a method of immunization or a method of vaccination using the immunogenic compositions or the vaccine compositions according to the invention, respectively.
The method includes at least one administration to an animal of an efficient amount of the immunogenic composition or vaccine according to the invention. The animal may be male or female. This administration may be notably done by intramuscular (IM), intradermal (ID) or subcutaneous (SC) injection or via intranasal or oral administration, wherein oral administration includes but is not limited to administration on feed or in drinking water, gels, or sprays. The immunogenic composition or the vaccine according to the invention can be administered by a syringe or a needleless apparatus (like for example Pigjet or Biojector (Bioject, Oregon, USA)). In an advantageous embodiment, the administration is oral.
The compositions according to the invention may also be administered to other mammals, e.g. mice or laboratory animal, for instance to generate polyclonal antibodies, or to prepare hybridomas for monoclonal antibodies.
The present invention provides for the immunization of animals, advantageously avians. Methods for administering coccidiosis vaccines are described in U.S. Pat. Nos. 4,438,097; 4,639,372; 4,808,404; 5,055,292; 5,068,104; 5,387,414; 5,602,033; 5,614,195; 5,635,181; 5,637,487; 5,674,484; 5,677,438; 5,709,862; 5,780,289; 5,795,741; 5,814,320; 5,843,722; 5,846,527; 5,885,568; 5,932,225; 6,001,363 and 6,100,241, the disclosures of which are incorporated by reference in their entireties. The method comprises administering to the animal an effective immunizing dose of the vaccine of the present invention. The vaccine may be administered by any of the methods well known to those skilled in the art, for example, by intramuscular, subcutaneous, intraperitoneal, intravenous, intranasally, orally, intradermal, intrabursal (just above the chickens vent), in ovo, or ocularly. Methods of administration are known to those skilled in the art. For example, U.S. Pat. Nos. 5,693,622; 5,589,466; 5,580,859; and 5,566,064 are hereby incorporated by reference in their entirety. Birds may also be administered vaccines in a spray cabinet. Birds may also be administered the vaccine in ovo, as described in U.S. Pat. Nos. 4,458,630 and 6,627,205, the disclosures of which are incorporated by reference.
Advantageously, birds are administered vaccines in a spray cabinet, i.e., a cabinet in which the birds are placed and exposed to a vapor containing vaccine, or by course spray. In another advantageous embodiment, the immunogenic or vaccine composition is administered orally. Alternatively, the immunogenic or vaccine composition can be administered in the drinking water or the feed.
The invention encompasses an immunogenic or vaccine composition comprising a mixture of sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella. The sporulated oocysts from precocious strains of E. acervulina, E. maxima and E. tenella are isolated from the seed cultures described herein. Generally, the dose range of sporulated oocysts in the composition is about 10 to about 1000 oocysts of E. acervulina, about 10 to about 100 oocysts of E. maxima and about 10 to about 1000 oocysts of E. tenella. Advantageously, the dose range of sporulated oocysts in the composition is about 125 to about 500 oocysts of E. acervulina, about 25 to about 100 oocysts of E. maxima and about 25 to about 500 oocysts of E. tenella. In one embodiment, a low dose is about 125 oocysts of E. acervulina, about 25 oocysts of E. maxima and about 25 oocysts of E. tenella. In another embodiment, a medium dose is about 250 oocysts of E. acervulina, about 50 oocysts of E. maxima and about 50 oocysts of E. tenella. In yet another embodiment, a high dose is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. 
Advantageously, the dose of the immunogenic or vaccine composition is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 500 oocysts of E. tenella. In a more advantageous embodiment, the dose is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella. 
The mixture optionally may comprise about 10 to about 1000 oocysts of E. mitis, advantageously about 125 to about 500 oocysts of E. mitis, about 125 oocysts of E. mitis in a low dose, about 250 oocysts of E. mitis in a medium dose and about 500 oocysts of E. mitis in a high dose.
The invention also relates to specific ratios of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella in a dose of the immunogenic or vaccine composition, wherein the ratio of E. acervulina:E. maxima E. tenella is about 10:1 to 2:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima E. tenella is about 5:1:1 (i.e., 10:2:2).
In an embodiment containing E. mitis, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 10:1 to 2:10:2 to 10 (i.e., for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima, about 10 sporocysts of E. mitis and about 2 to 10 sporocysts of E. tenella). Advantageously, the ratio of E. acervulina:E. maxima:E. mitis:E. tenella is about 5:1:5:1 (i.e., 10: 2:10:2).
Advantageously, the oocysts are suspended in a preservative consisting of a 0.01M phosphate buffered saline solution containing gentamicin. In another embodiment, the oocysts are suspended in any one of a variety of preservatives or organic acids such as, but not limited to, acetic acid, citric acid, potassium dichromate or propionic acid. For example, but not by limitation, sufficient sterile, 0.01M phosphate buffered saline containing not more than 30 mcg/ml gentamicin, is used to yield 2 ml per bottle for a 2,000 dose presentation, 5 ml per bottle for a 5,000 dose presentation and 10 ml per bottle for a 10,000 dose presentation. Advantageously, the oocysts are stored in sterile, borosilicate glass vials. For example, but not by limitation, the oocysts are aseptically filled into vaccine vials with a semi-automatic or automatic dispenser, stoppers are mechanically or manually inserted and aluminum seals are placed and crimped.
The vaccine is marketed as a multiple dose containing, 2000 dose vials, 5000 dose vials, 010,000 dose vials or 20,000 dose vials. The expiration date of the product shall not exceed 13 months from the date of the potency test initiation.
Animals, advantageously avians, can be vaccinated at any suitable age, and are usually about one to three days old before first vaccination. Advantageously, the animals are vaccinated once. Optionally, when two doses of vaccine are used, the first is normally given when the animals are 3 days to a week old and subsequently after a further 1-10 weeks dependent upon the type of animal being vaccinated.
The use, dosage and route of administration for each animal species in an advantageous embodiment is as follows. The immunogenic or vaccine composition of the present invention was used for the vaccination of healthy chickens one day of age or older, as an aid in the prevention of disease due to coccidiosis. Advantageously, the dosage was one dose per chicken by coarse water spray of 20 ml per 100 chickens.
The invention will now be further described by way of the following non-limiting examples.
EXAMPLES
Example 1
Cocci Production
Composition of the Product. The microorganisms that may be used are Eimeria acervulina, Eimeria maxima, Eimeria mitis and Eimeria tenella. The origins of the stock cultures of the microorganisms are as follows. The parent of the Eimeria acervulina was obtained from T. K. Jeffers at Hess and Clark Laboratories in 1969 and thought to have been isolated by Dr. M. Farr at USDA, Beltsville, Md., which was derived from a single oocyst. The Eimeria maxima culture was derived from an interbred mixture of 10 purified isolates obtained from Georgia, Delaware, Maryland, Virginia and Texas. The parent of the Eimeria mitis culture was isolated from Gainesville, Ga. in July 1978 and was purified by single oocyst isolation. The parent of the Eimeria tenella culture was obtained from a culture maintained at Pennsylvania State University by Dr. Patten since the early 1960's, and was acquired by the University of Georgia in 1982.
Each of the Eimeria strains used was a precocious strain of the respective microorganism as described in Avian Pathology, 17: 305-314, 1988 entitled “Eimeria of American Chickens: Characteristics of Six Attenuated Strains Produced by Selection for Precocious Development, P. L. Long and Joyce K. Johnson, the disclosure of which is incorporated by reference in its entirety. The microorganisms were attenuated by their selection for precocious development according to this method.
Cultures. Each Eimeria species used in the product was identified by their unique microscopic appearance, size and shape, and the zone of the chicken intestine or ceca infected, as described in Long, P. L. and W. M. Reid, A guide for the diagnosis of cocciodiosis in chickens. Re. Report 404 (Report 355 revised), Athens, Ga.: College of Agriculture Experiment Station, Univ. of Georgia; 1982, the disclosure of which is incorporated by reference in its entirety.
The microorganisms were attenuated by their selection for precocious development as described above. Each culture was free of pathogens by testing using the procedures described in 9 CFR 113.37. The stock cultures described above were maintained in the liquid or vapor phase of liquid nitrogen.
Chickens, 2 to 8 weeks of age, were used for the production of seed cultures. The route of administration was per os (i.e., orally). All seeds were sporulated oocysts and are produced in SPF chickens, 2 to 8 weeks of age. Dedicated facilities were maintained for each Eimeria species. A sufficient volume of sporulated oocysts (seed) was mixed with feed, free of anticoccidials, or was administered by oral gavage to provide each chicken with the minimum dose listed in Table 1. Sporulated oocysts were passed successively, without limitations to the passage, in 2 to 8 week old SPF chickens until the number of oocysts were sufficient to be used as seed for production. The cultures cannot be held for longer than 12 months in order to maintain viability/infectivity.







TABLE 1







Minimum dosage of sporulated oocysts (seed)










species
oocysts per chicken







E. acervulina
100 to 15,000



E. maxima
100 to 15,000



E. mitis
100 to 15,000



E. tenella
100 to 15,000










Droppings were collected daily from the third to the eighth day after inoculation. Random chickens were sacrificed and observed for characteristic infection for each species with the exception of mitis. No harvest was made if there was any evidence of extraneous disease.
The stock cultures for reference were maintained in liquid nitrogen. Production cultures were maintained a 5±3° C., and were passed in SPF chickens two to eight weeks of age.
The preparation of suspensions for seeding or inoculation involved serial passage of seed cultures in SPF Chickens, two to eight weeks of age, until sufficient oocysts were produced for manufacture.
A sufficient volume of sporulated oocysts (seed) was mixed with feed, free of anticoccidials, and administered to provide each chicken with the minimum dose listed in Table 1. Droppings were collected as one bath from the third to the eighth day after inoculation. Random chickens were sacrificed and observed for characteristic infection for each species with the exception of mitis. No harvest was made if there was any evidence of extraneous disease. The microorganisms are attenuated by their selection for precocious development as described above.
Harvest. Prior to removal of microorganisms, for production purposes, chickens were housed in a room designated for the production of individual species. The chickens were maintained on a diet free of anticocciodicals, and were allowed free access to water. Droppings were collected daily from the third to the eighth day after inoculation, and were held at 5±3° C. until processed.
The technique of harvesting microorganisms for production purposes were harvested as follows. Droppings were homogenized at an approximate ratio of 10% (w/v) in water. Large particles were removed by passing homogenate through screens. Solids were separated by either centrifugation, screening or by holding at 5±3° C. up to 24 hours. If solids were separated by holding at 5±3° C., they were further concentrated by centrifugation. The supernatant was discarded, and the solids were resuspended in a saturated NaCl (80% w/v) solution in water. The resulting solution was centrifuged. The oocysts were collected (removed) from the top of the liquid, and resuspended in water. Optimally, the remaining liquid was diluted to 20-40% NaCl with water and centrifuged. The pellet was then resuspended in a saturated NaCl solution and re-centrifuged, until no additional oocysts are recovered. The oocysts were washed no more than twice. The oocysts were washed free of salt by repeated, resuspension centrifugation cycles followed by resuspension in a 0.5% solution of sodium hypochlorite for 10 to 15 minutes. The oocysts were then washed free of the sodium hypochlorite solution by repeated (3×) centrifugation and resuspension steps. The final resuspension was made in a 2.5% aqueous solution of potassium dichromate (K2CrO7). The oocysts were then transferred to sporulation vessels. Sporulation was facilitated by sparging the suspensions with air for a period not to exceed 72 hours at 27±3° C. Following sporulation the oocysts were held at 5±3° C. until the final product was produced.
In an advantageous embodiment, the specifications for an acceptable harvest was as follows. First, the ratio of sporulated oocysts to total oocysts is determined. Only harvests meeting or exceeding >40% sporulation were considered acceptable. Second, the size, shape and appearance of each oocyst harvest must be characteristic of the species intended to be produced.
Discarded material not used in production was disposed of in a manner consistent with 9 CFR 114.15.
Preparation of the product. Serials or subserials of final product were assembled using bulk harvest fluids. All oocysts were attenuated, in that they are precocious, as described above. The oocysts were suspended in a preservative consisting of a 0.01M phosphate buffered saline solution containing not more than 30.0 mcg gentamicin. The product was standardized to yield at least the final numbers of sporulated oocycts of each species as indicated in Table 2.







TABLE 2







Standardized dosage of sporulated oocysts










species
oocysts per dose














E. acervulina
500



E. maxima
50



E. mitis
500



E. tenella
500










A serial was assembled as follows. Bulk suspension lots of each of the four Eimeria species are removed from 5±3° C. storage and allowed to warm to ambient room temperature while stirring. A sufficient volume of each lot(s) was aseptically removed and combined, to yield not less than the oocysts per dose listed above in Table 2, as determined by calculations based on counts of each of the bulk suspensions. If necessary, multiple lots of each Eimeria species were combined to provide the required number of sporulated oocysts. Sufficient sterile, 0.01M phosphate buffered saline containing not more than 30 mcg/ml Gentamicin, to yield 2 ml per bottle for a 2,000 dose presentation, 5 ml per bottle was used for a 5,000 dose presentation and 10 ml per bottle for a 10,000 dose presentation. Bulk serial was maintained under constant agitation.







TABLE 3







Example of serial volume (based on a 10,000,000 dose batch of


10,000 dose product)









Volume














E. acervulina
  500 ml @ 1.0 × 107 oocycts/ml



E. maxima
  300 ml @ 1.67 × 106 oocycts/ml



E. mitis
  750 ml @ 6.7 × 106 oocysts/ml



E. tenella
  1050 ml @ 2.4 × 106 oocycts/ml



0.01 M PBS/Gentamicin
17,400 ml



Total Volume
20,000 ml










An average 10,000-dose serial ranges from 20 L to 40 L. The fill volume is 1.0 ml per 1000 doses. The vaccine bottles are sterile, borosilicate glass vials.
The method and technique of filling and sealing final containers was as follows. The vaccine was aseptically filled into vaccine vials with a semi-automatic or automatic dispenser. Stoppers were mechanically or manually inserted. Aluminum seals are placed and crimped.
Each dose contained no less that the listed dose in Table 2 of sporulated oocysts, as calculated from bulk counts. A serial number was assigned to each quantity batched and filed in a single operation.
Testing. Each serial was tested for purity using the test methods described in 9 CFR 113.27 (e). The average count at either incubation condition should not exceed 1 colony per dose. If the average count at either incubation exceeded one colony per dose in the initial test, one retest was optionally conducted using 20 unopened final containers. If the average count at either incubation condition of the final test exceeded one colony per dose, the serial was unsatisfactory.
Salmonella was shown to be effectively killed by both sodium hypochlorite and by potassium dichromate, therefore, no additional testing was conducted. Mycoplasma was shown to be effectively killed by sodium hypochlorite, therefore, no additional testing was conducted.
Each serial was tested for extraneous pathogens in accordance with 9 CFR 113.37.
To test the safety of the vaccine, each of at least 25 one-day-old, specific pathogen free chickens was vaccinated by spray vaccination with 10 doses of vaccine. The chickens were observed each day for 21 days. Chickens that died during the period were examined and the cause of death was determined. If at least 20 chickens did not survive the observation period, the test was inconclusive. If any disease or death was directly attributable to the vaccine, the serial is unsatisfactory. Mild intestional lesions characteristic of the vaccine were considered normal, and are not considered in the safety evaluation. If less than 20 chicks survived the observation period and there were no deaths or severe lesions attributable to the vaccine, the test was optionally repeated one time. If the test is not repeated, the serial was declared unsatisfactory.
The first serial of final product produced from each new batch of production seed will be tested for potency. The product will be tested in SPF or broiler type chickens 1 to 14 days of age. Subsequent serials produced from the production seed will be evaluated for potency using pre-formulation counts specified above and recovery of oocysts from inoculated birds. No more that 70 chickens were used. No more than 35 were vaccinated per os with one field dose of vaccine. Twenty six to thirty days following the initial vaccination all chickens were individually identified and weights recorded. No more that 10 vaccinates and 10 controls from each of the groups listed below, were challenged per os, with 1.0 ml of each of the doses of challenge as shown in Table 4.







TABLE 4







Challenge doses










species
challenge dose














Group 1 challenge
E. acervulina
100,000 to 500,000
oocycts



E. maxima
10,000 to 1,000,000
oocycts


Group 2 challenge
E. mitis
100,000 to 500,000
oocysts


Group 3 challenge
E. tenella
10,000 to 100,000
oocycts









Challenge dose was also selected on the basis of pathogenicity in that the level chosen will give a minimum score of at least two.
Six days following challenge, the vaccinates and controls from each of the Groups in Table 4 were sacrificed, weighed, and the intestines and ceca were examined and scored for lesions. Scoring was in accordance with the Johnson and Reid cocciodial scoring system, as described in Experimental Parisitology, Vol. 28, p 30-36, 1970, the disclosure of which is incorporated by reference in its entirety, as shown in Table 5.







TABLE 5







Scoring of lesions








score
lesion











0
None


+1
Mild lesions


+2
Moderate


+3
Severe


+4
Extremely severe lesions or death









Post Preparatory Steps. The vaccine is marketed as a multiple dose containing, 2000 dose vials, 5000 dose vials, 010,000 dose vials or 20,000 dose vials. Collection, storage and submission of representative samples were in accordance with 9 CFR 113.3. The expiration date of the product did not exceed 13 months from the date of the potency test initiation, and confirmed in accordance with 9 CFR 114.13.
The use, dosage and route of administration for each animal species was as follows. This product was used for the vaccination of healthy chickens 1 day of age or older, as an aid in the prevention of disease due to coccidiosis. The dosage was one dose per chicken by coarse water spray; 20 ml per 100 chickens.
Example 2
Efficacy of Experimental Vaccine Containing Attenuated Strains of Avian Coccidia Compared with a Commercial Live Coccidiosis Vaccine and Salinomycin, an Anticoccidial Drug in Commercial Birds
The objective of this Example was the determination of the efficacy of attenuated Eimeria oocysts vaccine, in comparison with a USDA approved live oocyst vaccine (Coccivac-B) for protection of broiler type chickens from challenge with virulent Eimeria species and to compare the use of the attenuated vaccine with a conventional anticoccidial drug, Salinomycin.
The target variables were as follows. The primary variable was intestinal lesion scores. Acceptance criteria was lower post-challenge intestinal coccidial lesion scores when compared to the unvaccinated, challenged control birds. Secondary variables were (1) weight gain and feed conversion during a 7-week grow-out phase and (2) mortality. Acceptance criteria included equal or superior weight gain and feed conversion in vaccinated birds vs. birds vaccinated with CoccivacB or medicated with the anticoccidial drug and low mortality in vaccinated birds vs. unvaccinated control birds, CoccivacB vaccinated birds, and anticoccidial-treated birds.
Materials. The vaccines are described in Table 6.







TABLE 6







Vaccines










Experimental vaccine
Coccivac













Scientific
Eimeria acervulina, E.
Eimeria acervulina,


Name
tenella, E. maxima,
E. maxima, E. tenella,



E. mitis
E. mivati


Manufacturer
developed from Long's
Schering Plough



Attenuated Strains


Formulation
Refrigerated Suspensions


Preparation
Oocysts were prepared
Proprietary information



by oral inoculation



of susceptible chickens,



recovery of the oocysts



from feces, sporulation,



counting, and dilution



to appropriate inoculation



dose


Expiration Date
Approximately one year
Approximately one year


Storage
4°–10° C.—stored in
4°–10° C.—stored in


Requirements
1.5% potassium di-
1.5% potassium di-



chromate for approx-
chromate for approx-



imately 9 months
imately 9 months


Dose
Polyvalent inoculation
Proprietary info



with 1x vaccine, where



the 1x is defined as E.



tenella , 100 oocysts/



bird + E. acervulina



500 oocysts/bird + E.



mitis, 500 oocysts/bird +



E. maxima, 100 oocysts/



bird


Route of
Spra-Vac for oral
Oral administration


administration
administration
















TABLE 7





Virulent/Challenge Organisms
















Scientific Name
E. acervulina, E. mitis, E. tenella, E. maxima


Source
Field Isolates or Virulent Strains


Preparation
See Table 6


Storage Requirements
See Table 6


Dose
Polyvalent oral inoculation of susceptible



chickens with 20,000 to 50,000 oocysts of



E. tenella and E. maxima and 200,000



oocysts of E. mitis and E. acervulina


Route
Oral
















TABLE 8





Animals
















Species
Gallus domesticus (chicken)


Strain/breed
Commercial birds


Sex
F/M


Age
One-day


Number
Approximately 4000


Conditioning/
The chicks were placed in animal houses immediately


acclimation
after vaccination









Animals were managed similarly and with due regard for their well-being. Animals were handled in compliance with all applicable regulations.
Experimental Design. The experimental design was as follows.







TABLE 9







Experimental treatment groups
















Birds/
Reps/
Total
Route of




Group
Age
Rep
Grp
Birds
Admin
Inoculum
Treatment





1
1 Day
650
2
1300
Spray*
Experimental
Once








vaccine


2
1 Day
650
2
1300
Spray*
Coccivac-B
Once


3
1 Day
650
2
1300
N/A
Salinomycin
60 g/ton in feed each









day until Day 42 (stop









on Day 28 for the birds









removed for challenge)


4
1 Day
TBD
1
TBD
N/A
unvaccinated
N/A





*20 mls delivered per 100 chicks.






The timeline was as follows. On Day 0, birds were weighed by pen. The birds in Groups 1 and 2 were vaccinated as described in Table 9, with 1300 birds per vaccine regime, divided between 2 pens, one in each half of the house. The birds in Group 3 were started on Salinomycin, 60 g/ton, divided as described for Groups 1 and 2. The pens were stocked. Extra hatchmates were retained in wire cages as unvaccinated chicks.
On Day 21, the feed from starter to grower was changed for all birds. Salinomycin was continued in Group 3.
On Day 28, the birds to be challenged were moved to wire cages. Salinomycin was discontinued in Group 3 birds to be challenged. All birds and feed we weighed. Fresh fecal samples were collected from the litter from each group in the large pens, 20 samples per pen. Oocysts by quantified by species.
On Day 29, birds in the cages were challenged using the number of oocysts described in Table 7.
On Day 35, the challenge birds were terminated, the lesions were scored, and weight gains were taken.
On Day 42, all groups were changed to unmedicated finisher feed and the feed back was weighed.
On Day 49, all birds and feed were weighed and the trial was terminated.
Experimental Procedures.
Vaccination/Medication. Three treatments of birds identified as Groups 1-3 in Table 9, were replicated in half houses, three pens per house, 650 birds per pen, for a total of 1300 birds per treatment. Birds in Groups 1 through 3 were vaccinated as described in Table 9. An additional treatment group (4) was maintained separately in clean cages as unvaccinated, untreated controls, for use in the challenge test only.
Treatment Groups 1, 2, and 4 were given non-medicated feed. Treatment Group 3 was given Salinomycin (60 g/ton) beginning on Day 1 through Day 42. The birds in Group 3 to be challenged were given non-medicated feed after the move to the challenge cages. Starter feed was given until 21 days, grower until 42 days and finisher until 49 days. The feed issued was recorded, and any remaining unconsumed feed was weighed back at the time of feed changes and at termination.
Observations. Birds were weighed by pen at 0 and 28 days and by pen and sex at 49 days. Dead birds were collected twice daily and necropsy performed to determine cause of death. The fecal samples collected at Day 28 were observed for viable oocysts and quantitated by species.
Challenge. At Day 28, 3 reps of 10 birds from each pen (total of 60 per treatment) were weighed and moved to the battery cages for challenge with field strains of Eimeria (corresponding to the species used in the vaccine). All birds were given non-medicated feed in the cages. On Day 29, birds of Groups 1-4 were challenged and then terminated on Day 35. Lesion scores and weights were recorded.
The animals were randomly assigned to each treatment group by picking them up at random from the chick boxes before vaccinating. The scientists were not blind to the treatment groups since strict security was maintained between the groups to prevent cross contamination.
Concurrent Medications and Management of Adverse Events. The Coccivac vaccinated birds became sick 14 days into the trial. The diagnosis made was necrotic enteritis. The responsible agent was identified as Clostridium sordelli. All birds in the trial were administered Penicillin G to decrease contamination, and the affects thereof, between pens and to eliminate any difference in treatments among the groups that could skew the coccidiosis results.
Data Analysis.
Criteria for Measurement. The primary variables for the floor pen phase were live weights at 28 and 49 days and feed conversion at 49 days. For the challenge phase, primary variables were weight gain six days after challenge, and lesion scores of the upper, mid and cecal gut sections, also at six days after challenge.
Statistical Analysis. All statistical analyses were conducted using SAS, Cary, N.C. (Version 8.2). Statistical significance was based on two-tailed tests of null hypotheses that were examined in this study, resulting in p-values of 0.05 or less.
Intestinal Lesion Scores. The incidence and severity (categorical lesion scores) of post-challenge intestinal coccidial lesions were analyzed using a logit model with factors of treatment group and block and/or a survival analysis model with factors of treatment group and block (depending on the nature of the data).
Mortality. If mortality related to the virulent Eimeria species challenge occurred, an ANOVA (i.e., an analysis of variance) with factors of treatment group and block was conducted to determine if significant differences existed between the overall mortality means (regardless of the day of occurrence) for each treatment group.
Weight Gain and Feed Conversion.
Floor Pen Phase. Within treatment groups, paired t-tests (by block and as an entire group, without considering block effect) were conducted to determine if there are significant differences in mean live weights within each treatment group. Paired comparisons included Day 0 vs. Day 28 weights (pre-challenge) and Day 28 vs. Day 49 weights (post-challenge).
Paired t-tests (by block and as an entire group, without considering block effect) were conducted to determine if there are significant differences in mean feed conversion weights within each treatment group. Paired comparisons included Day 28 vs. Day 49 feed conversion weights (post-challenge) and Day 42 vs. Day 49 feed conversion weights (unmedicated finisher feed effect).
Between treatment groups, a repeated measures ANOVA with factors of treatment group, day, block, and group-day interaction was used to determine if significant differences existed in mean live weights between groups, on all days weights were recorded.
Challenge Phase. Within treatment groups, paired t-tests (by block and as an entire group, without considering block effect) were conducted to determine if there were significant differences in mean live weights within each treatment group. Paired comparisons included Day 28 vs. Day 35 weights (6 days post-challenge).
Between treatment groups, analysis of mean live weights between treatment groups, post-challenge, were conducted using the results of the between treatment group analysis for the floor pen phase, focusing on the Day 28 and Day 35 comparisons.
Results.







TABLE 10







28 Day Summary












Avg






Live

% All
Feed


Treatments
Wt
% Mortality (NE)*
Mortality
Conversion














EXPT.
1.41
0.23
2.69
1.429


VACCINE


COCCIVAC
1.39
11.77
15.69
1.453


SALINOMYCIN
1.45
0.46
3.76
1.383





*Signifies statistical differences by grouping.













TABLE 11







49 Day Summary















Avg Live
Avg

%
%



Avg Live
Female
Total
Feed
Mortality
Mortality


Treatments
Male Wt
Wt
Live Wt
Conversion
NE
Total
















EXPT.
3.21
2.62
2.90
1.84
0.23
4.69


VACCINE


COCCIVAC
3.11
2.55
2.82
1.92
11.76
17


SALINOMYCIN
3.17
2.63
2.90
1.86
0.53
5.38
















TABLE 12







Challenge Results

























%





6 Day
Upper
Middle
Ceca


Mortality



Initial
Final
Wt.
Lesion
Lesion
Lesion


Due to


Treatment
Wt.
Wt.
Gain
Score
Score
Score
TLS1
ATLS2
Coccidiosis



















Negative
1425.55ab*
1849.72a
424.17a
0.00c
0.00d
0.00d
0.00d
0.00d
0


Controls


Challenge
1396.95b
1538.75b
141.80c
2.48a
3.43a
2.38b
8.30a
2.77a
6.67


Controls


EXPT.
1459.60ab
1777.53a
317.93b
0.78b
1.30c
2.17b
5.25b
1.42b
0


VACCINE


COCCIVAC
1469.68a
1851.53a
381.85a
0.85b
0.93c
0.55c
2.33c
0.78c
0


SALINOMYCIN
1435.47ab
1488.97b
 53.50d
2.50a
2.90b
3.25a
8.65a
2.88a
0





60 birds were challenged per treatment.


*Signifies statistical differences by grouping.


a, b, c and d are Duncan's separation of means. (Duncan test is a statistical hoc test to separate the means).


1 is total lesion score


2 is average total lesion score






Discussion. There were no significant differences in the lesion scores for the experimental vaccine and Coccivac for the E. maxima, E. acervulina and E. mitis challenges. Coccivac did have lower lesions scores for E. tenella as compared to the experimental strains.
It is important to note that the Coccivac vaccinated birds became infected with Clostridium sordelli. Clostridial disease has been linked to infection with coccidiosis in the field. Even though the infection did spread to the other treatments, the Coccivac vaccinated birds were the most affected showing 11.77% mortality.
Conclusions. The experimental Eimeria strains were shown to be efficacious in the face of virulent challenge. The birds vaccinated with the experimental strain had better bird performance as defined by feed conversion rates when compared to Coccivac and Salinomycin treated birds at 49 days which corresponds to a economical advantage to the poultry producer.
Example 3
In vivo Potency Testing of Coccidiosis Vaccine, Live Oocysts, Chicken Origin
This Example presents a procedure to demonstrate the potency of harvest material from each new Eimeria production seed, as formulated in final product.
The materials for this Example include chicken feed, free of anticoccidials, SPF or broiler type chickens 1-14 days of age and chicken housing.
The first serial of final product produced and each subsequent serial produced using a new batch of production seed or seeds are tested for potency. The first serial produced, which contains all new production seeds are challenged with each species. For subsequent serials, which contain one or more new production seed(s), only those antigens representing the new seed(s) are tested for potency as described below. The final product is tested in SPF or broiler type chickens 1-14 days of age. Subsequent serials produced from the production seed are evaluated for potency using pre-formulation counts specified below.
The potency of E. acervulina, E. maxima and E. tenella are evaluated singularly, or in combination, in the same group of animals. For each strain tested, no more that 24 chickens are used. At least 10 and no more than 12 are individually vaccinated per os with one field dose of vaccine. Ten to 12 chickens will remain as controls. Twenty-six to 30 days following the initial vaccination all chickens are individually identified.
Each challenge strain used in the potency test must have a control group of at least 10 chickens.
At least 10 vaccinates and 10 controls of the applicable group listed below, are individually challenged per os, with 1.0 ml of homologous challenge or challenges at the dosage listed in Table 13







TABLE 13







Challenge












Species
Challenge Dose







Challenge
E. acervulina
100,000 to 500,000 oocysts



with



and/or
E. maxima
 10,000 to 1,000,000 oocysts



and/or
E. tenella
 10,000 to 1,000,000 oocysts










Challenge dose is also selected on the basis of Eimeria pathogenicity, in that the level chosen will cause at least 50% of the non-vaccinated challenge controls to have a lesion score of two or higher.
No group of vaccinates or controls can have less than eight animals.
Scoring is in accordance with the Johnson and Reid coccidial scoring system, as described in Experimental Parisitology, Vol. 28, p 30-36, 1970.







TABLE 14





Scoring
















0
None


+1
Mild lesions


+2
Moderate


+3
Severe


+4
Extremely, severe lesions or death









Six days following challenge, the vaccinates and controls are sacrificed, and the intestine and ceca are examined and scored for individual lesions according to each Eimeria species and the corresponding section of gut that correlates to infection i.e., E. acervulina infects upper intestine, E. maxima infects mid section of the gut and E. tenella infects the ceca.
Data are analyzed so that a significant difference between vaccinates and controls can be ascertained at the 0.05 level of significance using the Mann-Whitney U Test.
For each Eimeria species challenged for potency, total the respective individual lesion score for each of the vaccinates and controls. Each bird receives a score for E. maxima, a score for E. acervulina and a score for E. tenella. 
Rank scores of all animals from the smallest to the largest. For identical ranks, assign the average to each animal, e.g. if the 5th and 6th animal have an identical score, assign a rank of 5.5 to each one. Retain the identity of the rank as to whether it is a vaccinate or control.
Total the ranking numbers of the control group. Call this total “ΣRc.dd”
Total the ranking numbers of the vaccinate group. Call this total “ΣRv.”
Upon determining the sum of the ranks for the control group, the Mann-Whitney U Statistic of the controls (Uc) is computed using the following equation:




  
    U
    c
  
  =
  
    
      
        n
        c
      
      ⁢
      
        n
        v
      
    
    +
    
      
        
          n
          c
        
        ⁡
        
          (
          
            
              n
              c
            
            +
            1
          
          )
        
      
      2
    
    -
    
      ∑
      
        R
        c
      
    
  




Where nc represents the number of animals of the control group and nv represents the number of vaccinates.
Upon determining the sum of the ranks for the vaccinate group, the Mann-Whitney U Statistic of the controls (Uv) is computed using the following equation:




  
    U
    v
  
  =
  
    
      
        n
        v
      
      ⁢
      
        n
        c
      
    
    +
    
      
        
          n
          v
        
        ⁡
        
          (
          
            
              n
              v
            
            +
            1
          
          )
        
      
      2
    
    -
    
      ∑
      
        R
        v
      
    
  




Where nc represents the number of animals of the control group and nv represents the number of vaccinates.
The test is significant at the 0.05 level if Uc is less than Uv, and Uc is less than or equal to the critical value listed in Table 15 for the appropriate group sizes.







TABLE 15







Table of Critical values for Mann-Whitney U Statistic


(Two-Tailed 0.05 Values)









Uc
Controls (nc)
Vaccinates (nv)












≦23
10
10


≦20
9
10


≦17
8
10


≦20
10
9


≦17
9
9


≦15
8
9


≦17
10
8


≦15
9
8


≦13
8
8









Validity Criteria. For a valid test, at least 50% of the non-vaccinated challenge controls must have a lesion score of two or higher. No group of vaccinates or controls can have less than eight animals. If the animals meet the validity requirements and the computed Mann-Whitney U statistic is significantly different at the 0.05 level, the serial is satisfactory.
Re-test Requirements. If a vaccinate group fails to show a significant difference (the computed Uc statistic is greater than the critical values listed in Table 15) from the control group, the results of the test are inconclusive and the serial may be retested.
To determine if the serial was not satisfactory due to an over-challenge, the lesion scores of the vaccinates from first test session and the second test session are analyzed to determine if a significant difference between the sessions can be ascertained at the 0.05 level of significance using the Mann-Whitney U Test.
Undividual lesion scores per strain for the vaccinates from are totaled from both test sessions. Rank scores of all animals from the smallest to the largest. For identical ranks, assign the average to each animal, e.g. if the 5th and 6th animal have an idential score, assign a rank of 5.5 to each one. Retain the identity of the rank as to whether it is from the first or second test session.
Total the ranking numbers of the first test session. Call this total “ΣR1.”
Total the ranking numbers of the second test session. Call this total “ΣR2.”
Upon determining the sum of the ranks for the vaccinates from first test session, the Mann-Whitney U Statistic of the first test session (U1) is computed using the following equation:




  
    U
    1
  
  =
  
    
      
        n
        1
      
      ⁢
      
        n
        2
      
    
    +
    
      
        
          n
          1
        
        ⁡
        
          (
          
            
              n
              1
            
            +
            1
          
          )
        
      
      2
    
    -
    
      ∑
      
        R
        1
      
    
  




Upon determining the sum of the ranks for the vaccinate group of the second test session, the Mann-Whitney U Statistic of the controls (Uv) is computed using the following equation:




  
    U
    2
  
  =
  
    
      
        n
        2
      
      ⁢
      
        n
        1
      
    
    +
    
      
        
          n
          2
        
        ⁡
        
          (
          
            
              n
              2
            
            +
            1
          
          )
        
      
      2
    
    -
    
      ∑
      
        R
        2
      
    
  




Where n1 represents the number of animals of the vaccinates in the first test session and n2 represents the number of vaccinates in the second test session.
Interpretation of the Test Results. The test is significant at the 0.05 level if U1 is less than U2, and U2 is less than or equal to the critical value listed in Table 16 for the appropriate group sizes.







TABLE 16







Table of Critical values for Mann-Whitney U Statistic


(Two-Tailed 0.05 Values)









U1
Session #1 (n1)
Session #2 (n2)












≦23
10
10


≦20
9
10


≦17
8
10


≦20
10
9


≦17
9
9


≦15
8
9


≦17
10
8


≦15
9
8


≦13
8
8









If the results of the comparison of the first and second test sessions is significant at a 0.05 level, the initial result may be considered a “No-Test” due to over-challenge, and the vaccinates and controls will be compared using data generated from the second test session. The data analysis will be conducted as previously outlined.
If the comparison of the two test sessions fail to show significance at the 0.05 level, the data from both test sessions will be combined, and data will be analyzed as previously outlined.
The test is significant at the 0.05 level if Uc is less than Uv, and Uc is less than or equal to the critical value listed in Table 17 for the appropriate group sizes.







TABLE 17







Table of Critical values for Mann-Whitney U Statistic (Retest of Serial)









U1
Controls (n1)
Vaccinates (n2)












127
20
20


119
19
20


112
18
20


105
17
20


98
16
20


119
20
19


113
19
19


106
18
19


99
17
19


92
16
19


112
20
18


106
19
18


99
18
18


93
17
18


86
16
18


105
20
17


99
19
17


93
18
17


87
17
17


81
16
17


98
20
16


92
19
16


86
18
16


81
17
16


75
16
16









Reference: Sheskin, David J. Handbook of Parametric and Nonparametric Statistical Procedures, 3rd ed. 2003; 423-428, 1151.
Having thus described in detail advantageous embodiments of the present invention, it is to be understood that the invention is not to be limited to particular details set forth in the above description as many apparent variations thereof are possible without departing from the spirit or scope of the present invention.

## Claims

1. An immunogenic or vaccine composition for protection against E. acervulina, E. maxima and E. tenella consisting of a pharmaceutically acceptable excipient and a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein the mixture is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.

2. The composition of claim 1 wherein the mixture is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.

3. The composition of claim 1 wherein the mixture is about 500 oocysts of E. acervulina, about 50 oocysts of E. maxima and about 250 oocysts of E. tenella.

4. An immunogenic or vaccine composition for protection against E. acervulina, E. maxima and E. tenella consisting of a pharmaceutically acceptable excipient and a mixture of sporulated oocysts, wherein the mixture of sporulated oocytes consists of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella, wherein for every 10 sporocysts of E. acervulina, there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella.

5. The composition of claim 4 wherein for every 10 sporocysts of E. acervulina, there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella .

6. A method of eliciting an immune response comprising administering an effective amount of the immunogenic or vaccine composition of claim 1 to induce the response in a chicken.

7. A method for inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition of claim 1 to induce the response in a chicken.

8. A method of eliciting an immune response comprising administering an effective amount of the immunogenic or vaccine composition of claim 4 to induce the response in a chicken.

9. A method for inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition of claim 4 to induce the response in a chicken.

10. The method of claim 6 wherein the effective amount is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.

11. The method of claim 7 wherein the effective amount is about 500 oocysts of E. acervulina, about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.

12. The method of claim 6 wherein the effective amount is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.

13. The method of claim 7 wherein the effective amount is about 500 oocysts of E. acervulina, about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.

14. The method of claim 8 wherein the effective amount of the mixture of sporulated oocysts, wherein for every 10 sporocysts of E. acervulina, there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella in the mixture.

15. The method of claim 9 wherein the effective amount of the mixture of sporulated oocysts, wherein for every 10 sporocysts of E. acervulina, there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella in the mixture.

16. The method of claim 6 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.

17. The method of claim 7 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 100,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.

18. The method of claim 8 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.

19. The method of claim 9 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.