U.S. flag
        An official website of the United States government
          Here’s how you know
        Here’s how you know**Official websites use .gov**
              
              A **.gov** website belongs to an official government organization in the United States.
        **Secure .gov websites use HTTPS**
              
              A **lock** (  A locked padlock
) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. https://www.nist.gov/mml/biomolecular-measurement/applied-genetics/structure-cmv-genome
  Search NIST
    Search
              Menu

      


Close
    Topics
        All Topics
                                                        Advanced communications
                                                        Artificial intelligence
                                                        Bioscience
                                                        Buildings and construction
                                                        Chemistry
                                                        Climate
                                                        Cybersecurity
                                                        Electronics
                                                        EnergyEnvironment
                                                        Fire
                                                        Forensic science
                                                        Health
                                                        Information technology
                                                        Infrastructure
                                                        Manufacturing
                                                        Materials
                                                        Mathematics and statisticsMetrology
                                                        Nanotechnology
                                                        Neutron research
                                                        Performance excellence
                                                        Physics
                                                        Public safety
                                                        Resilience
                                                        Standards
                                                        Transportation
            Publications
            Labs & Major Programs
        Laboratories
                                Communications Technology Laboratory
                                  Engineering Laboratory
                                  Information Technology Laboratory
                                  Material Measurement Laboratory
                                  Physical Measurement LaboratoryUser Facilities
                                NIST Center for Neutron Research
                                  CNST NanoFab
                                                        Research Test Beds
                                                        Research Projects
                                                        Tools & InstrumentsMajor Programs
                                Baldrige Performance Excellence Program
                                  CHIPS for America Initiative
                                  Manufacturing Extension Partnership (MEP)
                                  Office of Advanced Manufacturing
                                  Special Programs Office
                                  Technology Partnerships Office
            Services & Resources
        Standards and Measurements
                                Calibration Services
                                  Laboratory Accreditation (NVLAP)
                                  Quality System
                                  Standard Reference Materials (SRMs)
                                  Standards.gov
                                  Time Services
                                  Office of Weights and MeasuresSoftware
                                                        Data
                                Chemistry WebBook
                                  National Vulnerability Database
                                  Physical Reference Data
                                  Standard Reference Data (SRD)
                                                        Storefront
                                                        License & PatentsComputer Security Resource Center (CSRC)
                                                        NIST Research Library
            News & Events
        News
                                                        Events
                                                        Blogs
                                                        Feature Stories
                                                        AwardsVideo Gallery
                                                        Image Gallery
                                                        Media Contacts
            About NIST
        About Us
                                Leadership
                                  Organization Structure
                                  Budget & Planning
                                                        Contact Us
                                                        Visit
                                                        Careers
                                Student programsWork with NIST
                                                        History
                                NIST Digital Archives
                                  NIST Museum
                                  NIST and the Nobel
                                                        Educational Resources
 
 Material Measurement Laboratory /                  Biomolecular Measurement Division
      Applied Genetics Group
    
      
  Applied Genetics Staff
          Clinical DNA Information Resource
          Publications & Presentations
          NRC  Postdoctoral Research OpportunitiesStructure of CMV Genome

Share
  Facebook
                      Linkedin
                      Twitter
                      Email

Structure of Cytomegalovirus Genome

Simplified genomic structure:

Structure of CMV Genome

Schematic map of the CMV genome. The CMV genome is organized as two regions of unique sequences, unique long (UL) and unique short (US), flanked by two sets of inverted repeats (TRL/IRL) and (IRS/TRS) (light shaded boxes). Kotenko et al. PNAS February 15, 2000 vol 97 no. 4 pp. 1695-1700



Nomenclature:

UL – Unique long
	US – Unique short
	TRL – Terminal repeat long
	IRL – Internal repeat long; inverted repeat of TRL
	TRS –Terminal repeat short
	IRS – Internal repeat short; inverted repeat of TRS

Detailed genomic structure:

CMV structure detailed

Map of the HCMV genome.  The green arrows represent previously annotated ORFs of HCMV likely to encode a *bona fide* polypeptide, red arrows designate ORFs unlikely to encode a polypeptide, and blue arrows indicated previously unrecognized ORFs that the present analysis predicts have high potential to encode proteins.  The gray box marks the additional sequence found in the HCMV Toledo strain, locating it with respect to the AD169 genome.  Rectangles superimposed on the line represent the sequence-identify terminal repeats.  Each mark on the sequence line represents 1,000 bp. Murphy et al. PNAS November 11, 2003 vol 100 no 23.

Alternate structures

CMV structure alternates

HCMV ORF organization.
	(A) Conventional ORF maps of the AD169 laboratory strain and clinical isolates.  From the left, the AD169 genome contains TRL1-14 (green arrow), UL1-132 (dark blue arrow), IRL14-1 (green arrow), IRS1 (red arrow), US1-36 (light blue arrow), and TRS1 (red arrow).  From the left, clinical isolates contain a unique domain including RL1-14 and UL1-151 (green plus dark blue arrow) followed by IRS1 (red arrow), US1-36 (light blue arrow), and TRS1 (red arrow).
	(B) ORF maps of the BAC clones whose sequences are reported in this article (see citation below).  Sequences were linearlized at the position corresponding to nucleotide 1 of the original AD169 sequence.  Arrows indicate the relative orientations of the repeated and unique ORF blocks.  The RL region is not repeated in the clinical isolates (Toledo, PH, TR, and FIX); there is a single copy of the region (green segment appended to the unique long region).  The Towne laboratory strain contains a block of ORFs (UL147-154, orange arrow) that is not present in AD169, and the clinical isolates contain a block of ORFs (UL133-151, orange arrow) that is not present in the AD169 laboratory strains.  The BAC inserts are identified (B), and viral ORFs deleted during BAC insertions are listed in parentheses.  Murphy et al. PNAS December 9, 2003 vol 100 no 25.
  Bioscience and Reference materials

    
    Contacts
      
        

Applied Genetics Group
        Megan Cleveland
    
      
  megan.cleveland@nist.gov
  (301) 975-5473

Created November 12, 2009, Updated November 15, 2019
 HEADQUARTERS
            100 Bureau Drive
              Gaithersburg, MD 20899301-975-2000
            Webmaster | Contact Us | Our Other Offices
        Twitter
            
Facebook
            
LinkedIn
            
Instagram
            
YouTube
            
Giphy
            
RSS Feed
            
Mailing List
                              How are we doing? Feedback

  
  Site Privacy
                      Accessibility
                      Privacy Program
                      Copyrights
                      Vulnerability Disclosure
                      No Fear Act Policy
                      FOIA
                      Environmental Policy
                      Scientific Integrity
                      Information Quality Standards
                      Commerce.gov
                      Science.gov
                      USA.gov
                      Vote.gov