# Patent

## Title
Compositions and methods for regulating production of a fusion protein and ribonucleic acid

## Abstract

The present disclosure relates to one or more agents, therapies, treatments, and methods of use of the agents and/or therapies and/or treatments for increasing production of a belatacept-similar protein and interfering RNA of tumor necrosis factor alpha. Embodiments of the present disclosure can be used as a therapy or a treatment for a subject that has a condition whereby the subject's immune system is, or is likely to become, dysregulated and where the production of the belatacept-similar protein and decreased production of tumor necrosis factor alpha may be of therapeutic benefit.

## Background
TECHNICAL FIELD
The present disclosure generally relates to compositions and methods for regulating production of a fusion protein and ribonucleic acid (RNA). In particular, the present disclosure relates to compositions and methods for regulating gene expression and, therefore, production of a fusion protein and interfering RNA both of which relate to suppressing immune responses.
BACKGROUND
The mammalian immune system can differentiate between self and foreign matter. A number of cascades of signaling molecules and immune cells are characterized by their ability to recognize foreign matter and to call upon the production and stimulation of effector cells of the immune system to kill, break down, consume, or sheath the foreign matter in order to protect a host.
It is known that under various conditions the immune system can become dysregulated. A dysregulated immune system can cause further damage to the host, thereby preventing healing. It may also result in a loss of homeostatic controls and/or a chronically stimulated immune system.
Known approaches to the treatment of conditions whereby the immune system is dysregulated are the commercially available pharmaceutical products that bind to and block the production or effectiveness of one or more checkpoint molecules.
SUMMARY
Some embodiments of the present disclosure relate to compositions and methods that upregulate the production of a belatacept-similar protein (BSP). The BSP has a similar, substantially the same or the same biological function as belatacept when administered to a subject. In some embodiments the compositions comprise vector of plasmid deoxyribonucleic acid (DNA) that includes an insert sequence of nucleic acids that encode for the production of the BSP and a backbone sequence of nucleic acids that facilitate introduction of the insert sequence into one or more of a subject's cells where the insert sequence is expressed and/or replicated. Expression of the insert sequence by one or more cells of the subject results in an increased production of the BSP. In some embodiments of the present disclosure, the methods that upregulate the production of BSP relate to methods of manufacturing and administering the composition.
Some embodiments of the present disclosure relate to compositions and methods that upregulate the production of one or more sequences of interfering ribonucleic acid (RNA). The sequences of RNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target cytokine and the interfering RNA can cause the target mRNA to be degraded or inactivated, thereby causing a decrease in production of the target cytokine. In some embodiments of the present disclosure, the target cytokine is a pro-inflammatory cytokine, such as tumor necrosis factor alpha (TNF-alpha). In some embodiments of the present disclosure, the target cytokine is an anti-inflammatory cytokine. In some embodiments of the present disclosure the compositions comprise vector DNA that includes one or more an insert sequences of nucleic acids that encode for the production of interfering RNA and a backbone sequence of nucleic acids that facilitates introduction of the one or more insert sequences into one or more of a subject's cells where it is expressed and/or replicated. Expression of the one or more insert sequences by one or more cells of the subject results in an increased production of the interfering RNA and, therefore, decreased translation of the target cytokine by one or more of the subject's cells. In some embodiments of the present disclosure, the methods that upregulate the production of the one or more interfering RNA sequences relate to methods of manufacturing and administering the composition.
Some embodiments of the present disclosure relate to compositions and methods that upregulate the production of both the BSP and interfering RNA that degrades, or causes degradation of, or inactivates or causes the inactivation of the mRNA of the target cytokine.
Some embodiments of the present disclosure relate to compositions and methods that can be used as a therapy or a treatment for a subject that has a condition whereby the subject's immune system is, or is likely to become, dysregulated.
Some embodiments of the present disclosure relate to a recombinant virus vector (RVV). The RVV comprises a nucleotide sequence encoding a belatacept-similar protein (BSP); one or more nucleotide sequences encoding an interfering ribonucleic acid (RNA) that targets messenger ribonucleic acid (mRNA) of tumor necrosis alpha (TNF-alpha); and an inverted terminal repeat.
Some embodiments of the present disclosure relate to a composition that comprises a nucleotide sequence according to the present disclosure (SEQ ID 7) that can be expressed in a target cell.
Some embodiments of the present disclosure relate to an insert for use with an RVV, wherein the insert has a nucleotide sequence according to SEQ ID 6.
Some embodiments of the present disclosure relate to a method of making an agent/target cell complex, the method comprising a step of administering a therapeutically effective amount of the agent to a subject, wherein the agent/target cell complex increases the subject's production of the BSP.
Some embodiments of the present disclosure relate to a method of making an agent/target cell complex, the method comprising a step of administering a therapeutically effective amount of the agent to a subject, wherein the agent/target cell complex increases the subject's production of one or more sequences of interfering RNA that decreases the production of a target cytokine.
Some embodiments of the present disclosure relate to a method of making an agent/target cell complex, the method comprising a step of administering a sufficient amount of an agent to a target cell whereby the agent/target cell complex is formed, wherein the agent/target cell complex increases the subject's production of the BSP and the agent/target cell complex also increases the subject's production of one or more sequences of interfering RNA that decreases the production of a target cytokine.
Some embodiments of the present disclosure relate to a pharmaceutical agent that comprises an agent, a pharmaceutically acceptable carrier and/or an excipient. Administering the pharmaceutical agent to a subject may increase the subject's production of the BSP, one or more sequences of interfering RNA that decreases the production of a target cytokine or both.
Some embodiments of the present disclosure relate to a method of treating a condition. The method comprises a step of administering to a subject a therapeutically effective amount of an agent that upregulates the subject's production of the BSP, one or more sequences of interfering RNA that decreases the production of a target cytokine or both.
Some embodiments of the present disclosure relate to a use of an agent for treating a condition, wherein the agent upregulates the subject's production of the BSP, one or more sequences of interfering RNA that decreases the production of a target cytokine or both.
Embodiments of the present disclosure relate to at least one approach for inducing endogenous production of the BSP and one or more sequences of interfering RNA that target the mRNA of TNF-alpha. A first approach utilizes gene vectors containing nucleotide sequences for increasing the endogenous production of BSP and one or more sequences of interfering RNA that target the mRNA of TNF-alpha, which can be administered to a subject to increase the subject's production of the BSP and one or more sequences of interfering RNA. Without being bound by any particular theory, embodiments of the present disclosure may be useful for treating conditions wherein the subject's immune system has become dysregulated.



BRIEF DESCRIPTION OF THE DRAWINGS
These and other features of the present disclosure will become more apparent in the following detailed description in which reference is made to the appended drawings.
FIG. 1 is a schematic that represents a first portion of a plasmid vector of SEQ ID 7, according to embodiments of the present disclosure.
FIG. 2 is a schematic that represents a second portion of a plasmid vector of SEQ ID 7, according to embodiments of the present disclosure, which is contiguous with the first portion of FIG. 1.
FIG. 3 is a schematic that represents a third portion of a plasmid vector of SEQ ID 7, according to embodiments of the present disclosure, which is contiguous with the second portion of FIG. 2.
FIG. 4 is a schematic that represents a fourth portion of a plasmid vector of SEQ ID 7, according to embodiments of the present disclosure, which is contiguous with the third portion of FIG. 3.
FIG. 5 is a schematic that represents a fifth portion of a plasmid vector of SEQ ID 7, according to embodiments of the present disclosure, which is contiguous with the fourth portion of FIG. 4.



DETAILED DESCRIPTION
Unless defined otherwise, all technical and scientific terms used herein have the meanings that would be commonly understood by one of skill in the art in the context of the present description. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present disclosure, the preferred methods and materials are now described. All publications mentioned herein are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited.
As used herein, the singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise. For example, reference to “an agent” includes one or more agents and reference to “a subject” or “the subject” includes one or more subjects.
As used herein, the terms “about” or “approximately” refer to within about 25%, preferably within about 20%, preferably within about 15%, preferably within about 10%, preferably within about 5% of a given value or range. It is understood that such a variation is always included in any given value provided herein, whether or not it is specifically referred to.
As used herein, the term “activity” is used interchangeably with the term “functionality” and both terms refer to the physiologic action of biomolecule.
As used herein, the term “agent” refers to a substance that, when administered to a subject, causes one or more chemical reactions and/or one or more physical reactions and/or or one or more physiological reactions and/or one or more immunological reactions in the subject. In some embodiments of the present disclosure, the agent is a plasmid vector.
As used herein, the term “ameliorate” refers to improve and/or to make better and/or to make more satisfactory.
As used herein, the term “biomolecule” refers to a carbohydrate, a protein, an amino acid sequence, a nucleic acid, a lipid, a primary metabolite, a secondary metabolite or another metabolite that is found within a subject. A biomolecule may be endogenous or exogenous to a subject.
As used herein, the term “cell” refers to a single cell as well as a plurality of cells or a population of the same cell type or different cell types. Administering an agent to a cell includes in vivo, in vitro and ex vivo administrations and/or combinations thereof.
As used herein, the term “complex” refers to an association, either direct or indirect, between one or more particles of an agent and one or more target cells. This association results in a change in the metabolism of the target cell. As used herein, the phrase “change in metabolism” refers to an increase or a decrease in the one or more target cells' production of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), one or more proteins, and/or any post-translational modifications of one or more proteins.
As used herein, the terms “dysregulation” and “dysregulated” refer to situations or conditions wherein homeostatic control systems have been disturbed and/or compromised so that one or more metabolic, physiologic and/or biochemical systems within a subject operate partially or entirely without said homeostatic control systems.
As used herein, the term “effector molecule” refers to a molecule within a subject that can directly or indirectly regulate the metabolic activity of a target cell by increasing or decreasing the production of DNA, RNA and/or amino-acid sequences and/or by increasing or decreasing any post-translational modifications of one or more proteins.
As used herein, the term “endogenous” refers to the production and/or modification of a molecule that originates within a subject.
As used herein, the term “excipient” refers to any substance, not itself an agent, which may be used as a component within a pharmaceutical composition or a medicament for administration of a therapeutically effective amount of the agent to a subject. Additionally, or alternatively, an excipient may, either alone or in combination with further chemical components, improve the handling and/or storage properties and/or permit or facilitate formation of a dose unit of the agent. Excipients include, but are not limited to, one or more of: a binder, a disintegrant, a diluent, a buffer, a taste enhancer, a solvent, a thickening agent, a gelling agent, a penetration enhancer, a solubilizing agent, a wetting agent, an antioxidant, a preservative, a surface active agent, a lubricant, an emollient, a substance that is added to mask or counteract a disagreeable odor, fragrance or taste, a substance added to improve appearance or texture of the composition and/or a substance that is used to form the pharmaceutical compositions or medicaments. Any such excipients can be used in any dosage forms according to the present disclosure. The foregoing classes of excipients are not meant to be exhaustive but are provided merely to be illustrative of what a person of skill in the art would know and would also recognize that additional types and combinations of excipients may be used to achieve delivery of a therapeutically effective amount of the agent to a subject through one or more routes of administration.
As used herein, the term “exogenous” refers to a molecule that is within a subject but that did not originate within the subject.
As used herein, the terms “inhibit”. “inhibiting”, and “inhibition” refer to a decrease in activity, response, or other biological parameter of a biologic process, disease, disorder or symptom thereof. This can include but is not limited to the complete ablation of the activity, response, condition, or disease. This may also include, for example, a 10% reduction in the activity, response, condition, or disease as compared to the native or control level. Thus, the reduction can be a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100, or any amount of reduction in between the specifically recited percentages, as compared to native or control levels.
As used herein, the term “medicament” refers to a medicine and/or pharmaceutical composition that comprises the agent and that can promote recovery from a disease, disorder or symptom thereof and/or that can prevent a disease, disorder or symptom thereof and/or that can inhibit the progression of a disease, disorder, or symptom thereof.
As used herein, the term “patient” refers to a subject that is afflicted with a disease or disorder. The term “patient” includes human and veterinary subjects.
As used herein, the term “pharmaceutical composition” means any composition comprising, but not necessarily limited to, an agent to be administered a subject in need of therapy or treatment of a disease, disorder or symptom thereof. Pharmaceutical compositions may include additives such as pharmaceutically acceptable carriers, pharmaceutically accepted salts, excipients and the like. Pharmaceutical compositions may also additionally include one or more further active ingredients such as antimicrobial agents, anti-inflammatory agents, anaesthetics, analgesics, and the like.
As used herein, the term “pharmaceutically acceptable carrier” refers to an essentially chemically inert and nontoxic component within a pharmaceutical composition or medicament that does not inhibit the effectiveness and/or safety of the agent. Some examples of pharmaceutically acceptable carriers and their formulations are described in Remington (1995, The Science and Practice of Pharmacy (19th ed.) ed. A. R. Gennaro, Mack Publishing Company. Easton, PA), the disclosure of which is incorporated herein by reference. Typically, an appropriate amount of a pharmaceutically acceptable carrier is used in the formulation to render said formulation isotonic. Examples of suitable pharmaceutically acceptable carriers include, but are not limited to: saline solutions, glycerol solutions, ethanol, N-(1(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium chloride (DOTMA), dioleolphosphotidylethanolamine (DOPE), and liposomes. Such pharmaceutical compositions contain a therapeutically effective amount of the agent, together with a suitable amount of one or more pharmaceutically acceptable carriers and/or excipients so as to provide a form suitable for proper administration to the subject. The formulation should suit the route of administration. For example, oral administration may require enteric coatings to protect the agent from degrading within portions of the subject's gastrointestinal tract. In another example, injectable routes of administration may be administered in a liposomal formulation to facilitate transport throughout a subject's vascular system and to facilitate delivery across cell membranes of targeted intracellular sites.
As used herein, the phrases “prevention of” and “preventing” refer to avoiding the onset or progression of a disease, disorder, or a symptom thereof.
As used herein, the terms “production”, “producing” and “produce” refer to the synthesis and/or replication of DNA, the transcription of one or more sequences of RNA, the translation of one or more amino acid sequences, the post-translational modifications of an amino acid sequence, and/or the production of one or more regulatory molecules that can influence the production and/or functionality of an effector molecule or an effector cell. For clarity, “production” is also be used herein to refer to the functionality of a regulatory molecule, unless the context reasonably indicates otherwise.
As used herein, the terms “promote”, “promotion”, and “promoting” refer to an increase in an activity, response, condition, disease process, or other biological parameter. This can include, but is not limited to, the initiation of the activity, response, condition, or disease process. This may also include, for example, a 10% increase in the activity, response, condition, or disease as compared to the native or control level. Thus, the increase in an activity, response, condition, disease, or other biological parameter can be 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, or more, including any amount of increase in between the specifically recited percentages, as compared to native or control levels.
As used herein, the term “prophylactic administration” refers to the administration of any composition to a subject, in the absence of any symptom or indication of a disease or disorder, to prevent the occurrence and/or progression of the disease or disorder within the subject.
As used herein, the terms “signal molecule”, “signalling molecule” and “regulatory molecule” can be used interchangeably and refer to a molecule that can directly or indirectly affect the production and/or functionality of an effector molecule or effector cell. Signal molecules can be enzymes or other types of biomolecules that can act as a direct ligand on a target cell or they may influence the levels or functionality of a downstream ligand or a receptor for a ligand.
As used herein, the term “subject” refers to any therapeutic target that receives the agent. The subject can be a vertebrate, for example, a mammal including a human. The term “subject” does not denote a particular age or sex. The term “subject” also refers to one or more cells of an organism, an in vitro culture of one or more tissue types, an in vitro culture of one or more cell types, ex vivo preparations, and for a sample of biological materials such as tissue and/or biological fluids.
As used herein, the term “target cell” refers to one or more cells and/or cell types that are deleteriously affected, either directly or indirectly, by a dysregulated immune system and/or a disease process. The term “target cell” also refers to cells that are not deleteriously affected but that are the cells in which it is desired that the agent interacts.
As used herein, the term “therapeutically effective amount” refers to the amount of the agent used that is of sufficient quantity to ameliorate, treat and/or inhibit one or more of a disease, disorder or a symptom thereof. The “therapeutically effective amount” will vary depending on the agent used, the route of administration of the agent and the severity of the disease, disorder or symptom thereof. The subject's age, weight and genetic make-up may also influence the amount of the agent that will be a therapeutically effective amount.
As used herein, the terms “treat”, “treatment” and “treating” refer to obtaining a desired pharmacologic and/or physiologic effect. The effect may be prophylactic in terms of completely or partially preventing an occurrence of a disease, disorder or symptom thereof and/or the effect may be therapeutic in providing a partial or complete amelioration or inhibition of a disease, disorder, or symptom thereof. Additionally, the term “treatment” refers to any treatment of a disease, disorder, or symptom thereof in a subject and includes: (a) preventing the disease from occurring in a subject which may be predisposed to the disease but has not yet been diagnosed as having it; (b) inhibiting the disease, i.e., arresting its development; and (c) ameliorating the disease.
As used herein, the terms “unit dosage form” and “unit dose” refer to a physically discrete unit that is suitable as a unitary dose for patients. Each unit contains a predetermined quantity of the agent and optionally, one or more suitable pharmaceutically acceptable carriers, one or more excipients, one or more additional active ingredients, or combinations thereof. The amount of agent within each unit is a therapeutically effective amount.
In embodiments of the present disclosure, the pharmaceutical compositions disclosed herein comprise an agent as described above in a total amount by weight of the composition of about 0.1% to about 95%. For example, the amount of the agent by weight of the pharmaceutical composition may be about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 1.1%, about 1.2%, about 1.3%, about 1.4%, about 1.5%, about 1.6%, about 1.7%, about 1.8%, about 1.9%, about 2%, about 2.1%, about 2.2%, about 2.3%, about 2.4%, about 2.5%, about 2.6%, about 2.7%, about 2.8%, about 2.9%, about 3%, about 3.1%, about 3.2%, about 3.3%, about 3.4%, about 3.5%, about 3.6%, about 3.7%, about 3.8%, about 3.9%, about 4%, about 4.1%, about 4.2%, about 4.3%, about 4.4%, about 4.5%, about 4.6%, about 4.7%, about 4.8%, about 4.9%, about 5%, about 5.1%, about 5.2%, about 5.3%, about 5.4%, about 5.5%, about 5.6%, about 5.7%, about 5.8%, about 5.9%, about 6%, about 6.1%, about 6.2%, about 6.3%, about 6.4%, about 6.5%, about 6.6%, about 6.7%, about 6.8%, about 6.9%, about 7%, about 7.1%, about 7.2%, about 7.3%, about 7.4%, about 7.5%, about 7.6%, about 7.7%, about 7.8%, about 7.9%, about 8%, about 8.1%, about 8.2%, about 8.3%, about 8.4%, about 8.5%, about 8.6%, about 8.7%, about 8.8%, about 8.9%, about 9%, about 9.1%, about 9.2%, about 9.3%, about 9.4%, about 9.5%, about 9.6%, about 9.7%, about 9.8%, about 9.9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 16%, about 17%, about 18%, about 19%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.
Where a range of values is provided herein, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the disclosure. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges, and are also, encompassed within the disclosure, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure.
In some embodiments of the present disclosure, an agent is a plasmid vector for introducing genes into a target cell for reproduction or transcription of an insert that comprises one or more nucleotide sequences that are carried within the plasmid vector. In some embodiments of the present disclosure, the plasmid vector is a viral vector. In some embodiments of the present disclosure, the vector is an adeno-associated virus vector.
In some embodiments of the present disclosure, the insert comprises one or more nucleotide sequences that encode for production of at least a belatacept similar protein (BSP).
The BSP has physiologic/biologic equivalence to belatacept, meaning the BSP will have substantially the same effect on the subject or target cell as a similar dose of belatacept will. Beletacept is a known immunosuppressant that is used to reduce rejection in recipients of organ transplants. Similar to belatacept, BSP is a fusion protein that combines an Fc portion of a human immunoglobulin IgG1 with an extracellular portion of cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Belatacept can interfere with binding of antigen presenting cells and, therefore, belatacept can prevent activation of T-cells. As such, the BSP may also prevent activation of T-cells.
In some embodiments of the present disclosure, the insert comprises one or more nucleotide sequences that encode for production of at least one sequence of interfering RNA that decreases the production of target cytokine proteins. The interfering RNA may, directly or indirectly, bind to and degrade the target mRNA or otherwise inactivate the target mRNA so that less or none of the target-cytokine protein is produced. In some embodiments of the present disclosure, the interfering RNA may be short-interfering RNA (siRNA), microRNA (miRNA) or combinations thereof.
In some embodiments of the present disclosure, the target cytokine is a pro-inflammatory cytokine, meaning it has the physiologic effect of increasing inflammatory processes in the subject. In some embodiments of the present disclosure, the target cytokine is TNF-alpha. In some embodiments of the present disclosure, the target cytokine is an anti-inflammatory cytokine.
In some embodiments of the present disclosure, the insert comprises two or more nucleotide sequences that each encode one or more interfering RNA sequences that may be complimentary to and degrades, or causes degradation of, mRNA of the target cytokine. In some embodiments of the present disclosure the insert comprises three nucleotide sequences that each encode an interfering RNA sequence that may be complimentary to and degrade, or causes degradation of, or inactivates or causes inactivation of mRNA of the target cytokine.
In some embodiments of the present disclosure, the insert comprises one or more nucleotide sequences that encode for production of the BSP and one or more nucleotide sequences that each encode for an interfering RNA sequence that may be complimentary to and degrades, or causes degradation of, or inactivates or causes inactivation of mRNA of the target cytokine. In some embodiments of the present disclosure, the insert comprises three nucleotide sequences that each encode for interfering RNA that may be complimentary to and degrade, or causes degradation of, or inactivate, or causes inactivation of mRNA of the target cytokine.
The present disclosure relates to one or more agents, therapies, treatments, and methods of use of the agents and/or therapies and/or treatments for initiating or upregulating production of the BSP while downregulating production and/or functionality of the target cytokine. Some embodiments of the present disclosure relate to methods for making a complex between at least one particle of an agent and at least one target cell of a subject for initiating or increasing production of the BSP and for downregulating the subject's production and/or functionality of the target cytokine. Embodiments of the present disclosure can be used as a therapy or a treatment for a subject that has a condition whereby the subject's immune system is, or is likely to become, dysregulated.
In some embodiments of the present disclosure, the agent can be administered to the subject by an intravenous route, an intramuscular route, an intraperitoneal route, an intrathecal route, an intravesical route, a topical route, an intranasal route, a transmucosal route, a pulmonary route, and combinations thereof.
In some embodiments of the present disclosure, the agent can be administered to the subject by pipetting a dose of the agent into an in vitro cell culture, perfusing or immersing an ex vivo cell or tissue preparation with a solution that comprises the agent, mixing a biological fluid sample with a solution or substrate that comprises the agent, or combinations thereof.
Some embodiments of the present disclosure relate to an agent that can be administered to a subject with the condition. When a therapeutically effective amount of the agent is administered to the subject, the subject may change production and/or functionality of one or more immune system molecules. For example, the subject may decrease production and/or functionality of one or more immune system signaling molecules and/or one or more immune system effector molecules by changing the production of one or more sequences of DNA, one or more sequences of RNA and/or one or more proteins and/or one or more regulatory molecules that regulate the levels and/or functionality of the subject's immune system signaling molecules and/or immune system effector molecules.
In some embodiments of the present disclosure, the subject may respond to receiving the therapeutic amount of the agent by changing production and/or functionality of one or more intermediary molecules by changing production of one or more DNA sequences, one or more RNA sequences, and/or one or more proteins that regulate the levels and/or functionality of the one or more intermediary molecules. The one or more intermediary molecules regulate the subject's levels and/or functionality of the one or more immune system signaling molecules and/or the one or more immune system effector molecules.
In some embodiments of the present disclosure, administering a therapeutic amount of the agent to a subject upregulates the production, functionality or both of the BSP and one or more sequences of interfering RNA that each target the mRNA of one or more target cytokines. Examples of the target cytokine include one or more pro-inflammatory cytokines, one or more anti-inflammatory cytokines or combinations thereof. In some embodiments of the present disclosure, there are one, two three, four, five, six, seven, eight, nine or ten interfering RNA sequences that each are complimentary to and degrade, or cause degradation of, one cytokine, such as TNF-alpha. In some embodiments of the present disclosure, the agent may comprise multiple copies of the same nucleotide sequence of interfering RNA.
In some embodiments of the present disclosure, the agent is a vector used for gene therapy. The gene therapy is useful for increasing the subject's endogenous production of the BSP and one or more sequences of interfering RNA that target the mRNA of a target cytokine. For example, the vector can contain one or more nucleotide sequences that that cause increased production of the BSP and increased production of one or more interfering RNA sequences that that each are complimentary to and degrade, or cause degradation of, or inactivate, or cause inactivation of one cytokine, such as TNF-alpha.
In some embodiments of the present disclosure, the vector used for gene therapy is a virus that can be enveloped or not, replication effective or not, or combinations thereof. In some embodiments of the present disclosure, the vector is a virus that is not enveloped and not replication effective. In some embodiments of the present disclosure, the vector is a virus of the Paroviridae family. In some embodiments of the present disclosure, the vector is a virus of the genus Dependoparvaovirus. In some embodiments of the present disclosure, the vector is an adeno-associated virus (AAV). In some embodiments of the present disclosure, the vector is a recombinant AAV.
The embodiments of the present disclosure also relate to administering a therapeutically effective amount of the agent. In some embodiments of the present disclosure, the therapeutically effective amount of the agent that is administered to a patient is between about 10 and about 1×1016 TCID50/kg (50% tissue culture infective dose per kilogram of the patient's body weight). In some embodiments of the present disclosure, the therapeutically effective amount of the agent that is administered to the patient is about 1×1013 TCID50/kg. In some embodiments of the present disclosure, the therapeutically effective amount of the agent that is administered to a patient is measured in TPC/kg (total particle count of the agent per kilogram of the patient's body weight). In some embodiments the therapeutically effective amount of the agent is between about 10 and about 1×1016 TCP/kg.
Some embodiments of the present disclosure relate to a method for making a complex within a subject. The method comprises a step of administering a therapeutically effective amount of the agent to the subject. The complex comprises at least one particle of agent and one or more target cells. When the complex is formed, it affects a change in metabolism of the one or more target cells, which results in the subject upregulating the production of the BSP and one or more sequences of interfering RNA that target the mRNA of a target cytokine, such as TNF-alpha. Examples of a target cell include, but are not limited to: an adrenal gland cell; a B cell; a bile duct cell; a chondrocyte; a cochlear cell; a corneal cell; an endocardium cell; an endometrial cell; an endothelial cell; an epithelial cell; an eosinophil; a fibroblast; a hair follicle cell; a hepatocyte; a lymph node cell; a macrophage; a mucosal cell; a myocyte; a neuron; a glomeruli cell; an optic nerve cell; an osteoblast; an ovarian tissue cell; a pancreatic islet beta cell; a pericardium cell; a platelet; a red blood cell (RBC); a retinal cell; a scleral cell; a Schwann cell; a T cell; a testicular tissue cell; a thyroid gland cell; a uveal cell; or combinations thereof.
Some embodiments of the present disclosure relate to a therapy, or method of treating a condition, that can be administered to a subject with the condition. The therapy comprises a step of administering to the subject a therapeutically effective amount of an agent that will upregulate the subject's production of the BSP and one or more sequences of interfering RNA that target the mRNA of a target cytokine, such as TNF-alpha The increased production of the BSP and increased production of the interfering RNA may reduce deleterious effects of the condition upon the subject.
Below are examples of nucleotide sequences of each may be present in the insert. As will be appreciated by those skilled in the art, minor modifications, substitutions or replacements of a select few nucleotides or amino acids in the sequences provided below will not substantially impact the physiologic or biologic effect of such modified sequences, as compared to the sequences provided herein below. Any such modified sequences are also contemplated by the present disclosure.








(nucleotide sequence for BSP)



SEQ ID 1



atgcacgtgg cccagcctgc tgtggtactg gccagcagcc gaggcatcgc cagctttgtg   60



 


tgtgagtatg catctccagg caaatacact gaggtccggg tgacagtgct tcggcaggct  120


 


gacagccagg tgactgaagt ctgtgcggca acctacatga tggggaatga gttgaccttc  180


 


ctagatgatt ccatctgcac gggcacctcc agtggaaatc aagtgaacct cactatccaa  240


 


ggactgaggg ccatggacac gggactctac atctgcaagg tggagctcat gtacccaccg  300


 


ccatactacg agggcatagg caacggaacc cagatttatg taattgatcc agaaccgtgc  360


 


ccagattctg atcag                                                   375


 


(nucleotide sequence for interfering RNA-E1 protein)


SEQ ID 2



cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgcgct   60



 


atctcatacc aggagaaata gtgaagccac agatgtattt ctcctggtat gagatagcat  120


 


gcctactgcc tcggacttca aggggctaga attcg


 


(nucleotide sequence for interfering RNA-E2 protein)


SEQ ID 3



cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgacaa   60



 


accaccaagt ggaggagcta gtgaagccac agatgtagct cctccacttg gtggtttgct  120


 


gcctactgcc tcggacttca aggggctaga attcg                             155


 


(nucleotide sequence for interfering RNA-E3 protein)


SEQ ID 4



cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgacca   60



 


agtacttaga ctttgcggta gtgaagccac agatgtaccg caaagtctaa gtacttgggt  120


 


gcctactgcc tcggacttca aggggctaga attcg                             155


 


(nucleotide sequence for interfering RNA-E1, 2 and 3 proteins)


SEQ ID 5



cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgcgct   60



 


atctcatacc aggagaaata gtgaagccac agatgtattt ctcctggtat gagatagcat  120


 


gcctactgcc tcggacttca aggggctaga attcgcgact tcttaaccca acagaaggct  180


 


cgagaaggta tattgctgtt gacagtgagc gacaaaccac caagtggagg agctagtgaa  240


 


gccacagatg tagctcctcc acttggtggt ttgctgccta ctgcctcgga cttcaagggg  300


 


ctagaattcg cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag  360


 


tgagcgacca agtacttaga ctttgcggta gtgaagccac agatgtaccg caaagtctaa  420


 


gtacttgggt gcctactgcc tcggacttca aggggctaga attcg                  465


 


(nucleotide sequence for insert with BSP, interfering RNA-E1,


2 and 3 proteins)


SEQ ID 6



ggtaccgcca ccatggccac cggctctcgc acaagcctgc tgctggcttt cggactgctg   60



 


tgcctgcctt ggctccagga gggctccgcc atgcacgtgg cccagcctgc tgtggtactg  120


 


gccagcagcc gaggcatcgc cagctttgtg tgtgagtatg catctccagg caaatacact  180


 


gaggtccggg tgacagtgct tcggcaggct gacagccagg tgactgaagt ctgtgcggca  240


 


acctacatga tggggaatga gttgaccttc ctagatgatt ccatctgcac gggcacctcc  300


 


agtggaaatc aagtgaacct cactatccaa ggactgaggg ccatggacac gggactctac  360


 


atctgcaagg tggagctcat gtacccaccg ccatactacg agggcatagg caacggaacc  420


 


cagatttatg taattgatcc agaaccgtgc ccagattctg atcagggatc cggaggagga  480


 


ggcagcggag gaggcggatc tggcggaggc ggaagcgaca agacccacac atgcccacca  540


 


tgtcctgcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag  600


 


gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac  660


 


gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag  720


 


acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc  780


 


ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc  840


 


ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg  900


 


tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct gacctgcctg  960


 


gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag 1020


 


aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc 1080


 


aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg 1140


 


catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatag 1200


 


cgctagccga cttcttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga 1260


 


gcgcgctatc tcataccagg agaaatagtg aagccacaga tgtatttctc ctggtatgag 1320


 


atagcatgcc tactgcctcg gacttcaagg ggctagaatt cgcgacttct taacccaaca 1380


 


gaaggctcga gaaggtatat tgctgttgac agtgagcgac aaaccaccaa gtggaggagc 1440


 


tagtgaagcc acagatgtag ctcctccact tggtggtttg ctgcctactg cctcggactt 1500


 


caaggggcta gaattcgcga cttcttaacc caacagaagg ctcgagaagg tatattgctg 1560


 


ttgacagtga gcgaccaagt acttagactt tgcggtagtg aagccacaga tgtaccgcaa 1620


 


agtctaagta cttgggtgcc tactgcctcg gacttcaagg ggctagaatt cgtctagaa  1679


 


(expression cassette with insert)


SEQ ID 7



cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg   60



 


acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc  120


 


atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca  150


 


tgctctagga cattgattat tgactagtgg agttccgcgt tacataactt acggtaaatg  240


 


gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc  300


 


ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa  360


 


ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca  420


 


atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta  480


 


cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt  540


 


tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt  600


 


tttaattatt ttgtgcagcg atgggggcgg gggggggggg gggcgcgcgc caggcggggc  660


 


ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag  720


 


cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa  780


 


gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc  840


 


cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactaaaac aggtaagtcc  900


 


ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg  960


 


ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 1020


 


cggcccgctg ctcataagac Icggccttag aaccccagta tcagcagaag gacattttag 1080


 


gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 1140


 


aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 1200


 


gatgcctcta ctaaccatgt tcatgttttc tttttttttc tacaggtcct gggtgacgaa 1260


 


cagggtaccg ccaccatggc caccggctct cgcacaagcc Igctgctggc tttcggactg 1320


 


ctgtgcctgc cttggctcca ggagggctcc gccatgcacg tggcccagcc tgctgtggta 1380


 


ctggccagca gccgaggcat cgccagcttt gtgtgtgagt atgcatctcc aggcaaatac 1440


 


actgaggtcc gggtgacagt gcttcggcag gctgacagcc aggtgactga agtctgtgcg 1500


 


gcaacctaca tgatggggaa tgagttgacc ttcctagatg attccatctg cacgggcacc 1560


 


tccagtggaa atcaagtgaa cctcactatc caaggactga gggccatgga cacgggactc 1620


 


tacatctgca aggtggagct catgtaccca ccgccatact acgagggcat aggcaacgga 1680


 


acccagattt atgtaattga tccagaaccg tgcccagatt ctgatcaggg atccggagga 1740


 


ggaggcagcg gaggaggcgg atctggcgga ggcggaagcg acaagaccca cacatgccca 1800


 


ccatgtcctg cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 1860


 


aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 1920


 


cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1980


 


aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 2040


 


gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 2100


 


ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag 2160


 


gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc 2220


 


ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 2280


 


gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 2340


 


agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 2400


 


atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa 2460


 


tagcgctagc cgacttctta acccaacaga aggctcgaga aggtatattg ctgttgacag 2520


 


tgagcgcgct atctcatacc aggagaaata gtgaagccac agatgtattt ctcctggtat 2580


 


gagatagcat gcctactgcc tcggacttca aggggctaga attcgcgact tcttaaccca 2640


 


acagaaggct cgagaaggta tattgctgtt gacagtgagc gacaaaccac caagtggagg 2700


 


agctagtgaa gccacagatg tagctcctcc acttggtggt ttgctgccta ctgcctcgga 2760


 


cttcaagggg ctagaattcg cgacttctta acccaacaga aggctcgaga aggtatattg 2820


 


ctgttgacag tgagcgacca agtacttaga ctttgcggta gtgaagccac agatgtaccg 2880


 


caaagtctaa gtacttgggt gcctactgcc tcggacttca aggggctaga attcgtctag 2940


 


aataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 3000


 


gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 3060


 


cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 3120


 


ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 3180


 


actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 3240


 


cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 3300


 


ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 3360


 


ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 3420


 


ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 3480


 


cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctaagctt 3540


 


atcgataccg tcgagatcta acttgtttat tgcagcttat aatggttaca aataaagcaa 3600


 


tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3660


 


caaactcatc aatgtatctt atcatgtctg gatctcgacc tcgactagag catggctacg 3720


 


tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg 3780


 


ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 3840


 


gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgccagctg gcgtaatagc 3900


 


gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaat 3960


 


tccagacgat tgagcgtcaa aatgtaggta tttccatgag cgtttttcct gttgcaatgg 4020


 


ctggcggtaa tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc 4080


 


aggcaagtga tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg 4140


 


gacagactct tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg 4200


 


taccgttcct gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta 4260


 


acgaggaaag cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc 4320


 


gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 4380


 


ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 4440


 


cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 4500


 


gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 4560


 


gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 4620


 


ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 4680


 


tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 4740


 


atattaacgt ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc 4800


 


tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat 4860


 


tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa 4920


 


aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg 4980


 


gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag 5040


 


gcattgcatt (aaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg 5100


 


cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat 5160


 


gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgrat gatttattgg 5220


 


atgttggaat tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 5280


 


atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 5340


 


ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 5400


 


caagctgtga ccgtctcogg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 5460


 


cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 5520


 


atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 5580


 


ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5640


 


cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 5700


 


cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 5760


 


aaagatgctg aagatcagtt gggtgcaoga gtgggttaca tcgaactgga tctcaacagc 5820


 


ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 5880


 


gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 5940


 


cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 6000


 


acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 6060


 


gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 6120


 


aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 6180


 


ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 6240


 


ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 6300


 


gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 6360


 


aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 6420


 


aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 6480


 


aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 6540


 


gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 6600


 


gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 6660


 


tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 6720


 


gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 6780


 


caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6840


 


actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6900


 


acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6960


 


cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 7020


 


gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 7080


 


cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 7140


 


gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 7200


 


tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 7260


 


tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 7320


 


gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 7380


 


aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 7440


 


agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 7500


 


cgttggccga ttcattaatg                                             7520


 


(expression cassette start to BSP region)


SEQ ID 8



ggtaccgcca ccatggccac cggctctcgc acaagcctgc tgctggcttt cggactgctg   60



 


tgcctgcctt ggctccagga gggctccgcc                                    90


 


(expression cassette end of BSP region to interfering R-E1)


SEQ ID 9



ggatccggag gaggaggcag cggaggaggc ggatctggcg gaggcggaag cgacaagacc   60



 


cacacatgcc caccatgtcc tgcacctgaa ctcctggggg gaccgtcagt cttcctcttc  120


 


cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg  180


 


gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag  240


 


gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc  300


 


agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc  360


 


tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc  420


 


cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc  480


 


agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc  540


 


aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc  600


 


ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc  660


 


tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg  720


 


tctccgggta aatagcgcta gc                                           742


 


(inverted terminal repeat)


SEQ ID 10



tctaga                                                               6



 


(amino acid sequence for BSP produced per SEQ ID 1)


SEQ ID 11



Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile



1                5                  10              15


 


Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Tyr Thr Glu Val


            20                  25                  30


 


Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cvs


        35                  40                  45


 


Ala Ala The Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser


 


    50                  55                  60


Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln


 


65                  70                  75                  80


Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu


 


                85                  90                  95


Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile


 


            100                 105                 110


Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Gln


        115                 120                 125






Example 1—Expression Cassette
Expression cassettes for expressing a monoclonal antibody (mAb) and/or a protein and/or interfering RNA were synthesized by Genscript. Each cassette contained a signal peptide, the variable heavy domain, the human IgG1 constant domain, the protein or the interfering RNA sequence followed by (when it is an Ab), a self-cleaving 2A peptide sequence, a signal peptide, the variable light domain and the human lambda constant domain. The synthesized mAb and/or protein and/or interfering RNA expression cassettes were cloned into the pAVA-00200 plasmid backbone containing the CASI promoter1, multiple cloning site (MCS), Woodchuck Hepatitis Virus post-transcriptional regulatory element (WPRE), Simian virus 40 (SV40) polyadenylation (polyA) sequence all flanked by the AAV2 inverted terminal repeats (ITR). pAVA-00200 was cut with the restriction enzymes KpnI and XbaI in the MCS and separated on a 1% agarose gel. The band of interest was excised and purified using a gel extraction kit. Each mAb and/or protein and/or interfering RNA expression cassette was amplified by PCR using Taq polymerase and the PCR products were gel purified and the bands on interest were also excised and purified using a gel extraction kit. These PCR products contained the mAb and/or protein and/or interfering RNA expression cassettes in addition to 15 base pair 5′ and 3′ overhangs that align with the ends of the linearized pAVA-00200 backbone. Using in-fusion cloning2, the amplified mAb or protein or interfering RNA expression cassettes are integrated with the pAVA-00200 backbone via homologous recombination. The resulting plasmid vectors contained the following 5′ ITR, CASI promoter, monoclonal antibody or protein or interfering RNA expression cassette, WPRE, SV40 polyA and ITR 3′, per SEQ ID 7 and as shown in five contiguous portions in FIG. 1, FIG. 2, FIG. 3, FIG. 4 and FIG. 5.
Example 2—Animal Studies
C57BL/6 mice and BALB/c mice were purchased from Charles River. AAV vectors of Example 1 were administered to 6-week-old C57BL/6 mice with the exception of the AAV vector that encoded mAb expression, which was tested in BALB/c mice. All animal experiments were approved by the institutional animal care committees of the Canadian Science Centre for Human and Animal Health and the University of Guelph. Intramuscular or intraorgan administration of the AAV were performed using a 29-gauge needle and a 40-μL injection volume. Injection into the tail vein was performed on mice that were warmed slightly, using a 100-μL injection volume. Intranasal administration of the AAV vectors were performed using a 40-μL injection volume. The dose used was about 2×1011 vector genomes per mouse.
Example 3—Experimental Data
Table 2 below summarizes the data obtained from mice that received a hind flank, intramuscular administration of an AAV vector that encoded the belatacept similar protein (BSP) and three sequences of interfering RNA that target production of TNF-alpha. These mice were compared against a control. The data below came from a muscle biopsy sample taken from the hind flank region on the same side where the mouse received the intramuscular injection.







TABLE 2







BSP and TNF-alpha.












Molecule
Control (ag)
AAV recipient (ag)
P-value
















BSP
0
9
0.0061



TNF-alpha
12
2
0.0151







n.b. ag = attogram 1 × 10−18 grams






As shown in Table 2, mice that received the AAV that included sequences that encoded for increased production of BSP demonstrated statistically significant higher amounts of BSP than the control mice that did not receive the AAV. Furthermore, mice that received the same AAV that encoded for increased production of three interfering RNA sequences also demonstrated a lower amount of TNF-alpha than the control mice. Without being bound by any particular theory, the mice who received the AAV had higher levels of BSP and lower TNF-alpha levels than mice that did not receive the AAV.

## Claims

1. A recombinant virus vector (RVV), the RVV comprising:
a) a nucleotide sequence encoding a belatacept-similar protein (BSP), wherein the nucleotide sequence encoding the BSP is SEQ ID NO: 1;
b) one or more nucleotide sequences encoding an interfering ribonucleic acid (RNA) that targets messenger ribonucleic acid (mRNA) of tumor necrosis alpha (TNF-alpha); and
c) an inverted terminal repeat.

2. The RVV of claim 1, wherein the one or more nucleotide sequences encoding the interfering RNA are one or more of SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4.

3. The RVV of claim 1, wherein the inverted terminal repeat is SEQ ID NO: 10.

4. The RVV of claim 3, wherein SEQ ID NO: 10 is adjacent a start region of the RVV, an end region of the RVV or both.

5. The RVV of claim 1, wherein the RVV comprises SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4.

6. The RVV of claim 1, further comprising a first sequence of nucleotides that is positioned between a start region of the RVV and the SEQ ID NO: 1, and the first sequence of nucleotides have 95-99% the same nucleotides as SEQ ID NO: 8.

7. The RVV of claim 6, wherein the one or more nucleotide sequences encoding the interfering RNA is SEQ ID NO: 2 and wherein the RVV of claim 6 further comprises a second sequence of nucleotides that is positioned between SEQ ID NO: 1 and SEQ ID NO: 2 and that have 95-99% the same nucleotides as SEQ ID NO: 9.

8. The RVV of claim 1, wherein the RVV is an adeno-associated virus.

9. A composition that comprises a nucleotide sequence that can be expressed in a target cell, wherein the nucleotide sequence is SEQ ID NO: 7.

10. An insert for use with a recombinant virus vector (RVV), wherein the insert has a nucleotide sequence that is SEQ ID NO: 6.