# Patent

## Title
ST-246 (tecovirimat monohydrate) suspension formulations

## Abstract

The present invention is directed to a dry suspension for reconstitution containing Tecovirimat (ST-246) powder and simethicone. The dry suspension is dispersed in water to provide an aqueous pharmaceutical suspension formulation for oral administration for treating orthopoxvirus infections and/or eczema vaccinatum. The suspension formulation exhibits excellent stability and good dissolution and has an improved taste and texture.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
The present application is a Continuation application of U.S. application Ser. No. 16/071,119 filed Jul. 19, 2018 which is a § 371 National Phase application based on PCT/US2017/017915 filed Feb. 15, 2017 which claims the benefit of U.S. provisional application No. 62/295,710 filed Feb. 16, 2016 the subject matter of each of which is incorporated by reference in their entirety.


STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
This invention was made with U.S. government support under Contract No.: IIIISO100201100001C awarded by the Biomedical Advanced Research and Development Authority (BARDA). The U.S. government has certain rights in this invention.


FIELD OF THE INVENTION
The present invention relates to a novel oral powder for suspension (dry suspension) containing Tecovirimat (ST-246) powder for reconstitution and a process for making the dry suspension. The dry suspensions are dispersed in water to provide aqueous pharmaceutical suspension formulations for oral administration.
BACKGROUND OF THE INVENTION
Throughout this application, various publications are referenced within the text. The disclosure of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art as known to those skilled in therein as of the date of the invention described and claimed herein.
Historically, variola virus, the etiologic agent of smallpox, has been estimated to have killed, crippled, or disfigured nearly 10% of the human population prior to eradication (1). Smallpox is highly communicable and carries exceptionally high morbidity. Secondary attack rates among unvaccinated members of households in which someone had smallpox have been reported to range from r30% to 80%. Mortality rates range from 1% for variola minor to 30% for variola major. With the advent of biowarfare as an instrument of terrorism, smallpox can no longer be thought of as a disease of historic impact only.
There are currently no therapies other than early vaccination that can alter the outcome of disease or potentially prevent disease in a population that has been exposed to smallpox. Vaccination carries an inherent risk of adverse events for certain immunosuppressed recipients and even some healthy recipients (2). Moreover, vaccination is effective only if administered within 4 days post-exposure. Thus, antiviral drugs used alone or potentially in combination with vaccination can be used to treat individuals during the window of vulnerability which occurs prior to development of protective immunity. Additionally, antiviral drugs could also be used in the treatment of zoonotic poxvirus disease in humans, such as monkeypox.
ST-246 (4-trifluoromethyl-N-(3,3a,4,4a,5,5a,6,6a-octahydro-1,3-dioxo-4,6-ethenocycloprop[f]isoindol-2(III)-yl)-benzamide)(Tecrovirmat), has recently emerged as a potent candidate against orthopoxvirus. Several studies evaluating ST-246 for activity against orthopoxviruses have demonstrated excellent in vitro and in vivo efficacy (3, 4). When evaluated in vitro against vaccinia virus (VV), cowpox virus (CV), ectromelia virus (ECTV), monkeypox, camelpox, and variola viruses, ST-246 inhibited virus replication by 50% (50% effective concentration [EC50]) at or below a concentration of 0.07 μM. With animal models using lethal infections with ECTV, VV, or CV, ST-246 was reported to be nontoxic and highly effective in preventing or reducing mortality even when treatments were delayed up to 72 h post-viral inoculation (3, 4). ST-246 was also evaluated with the nonlethal mouse tail lesion model using intravenous VV. When ST-246 was administered orally twice a day at 15 or 50 mg/kg of body weight for 5 days, the tail lesions were significantly reduced (4). Most recently, an infant was given ST-246 as an FDA-authorized emergency treatment for eczema vaccinatum which developed after exposure to the parent's predeployment military smallpox immunization (5).
Given the high efficacy of ST-246 antiviral therapy against smallpox and a lack of FDA-approved medications for the treatment of smallpox infection, there is clearly a need for developing safe and effective ST-246 formulations that can be administered by various routes of administration. However, the poor solubility of ST-246 in water and in pharmaceutically acceptable pH buffers and commonly used pharmaceutical vehicles such as co-solvents, surfactants, complexing agents, and lipids creates an impediment to making safe and effective ST-246 liquid formulations.
Thus, there is a critical need in the pharmaceutical and other biological based industries to formulate water insoluble ST-246 into liquid suspensions for oral, parenteral, or topical administration.
However, the preparation of an aqueous pharmaceutical suspension formulation from micronized ST-246 presents problems that are difficult to overcome, such as retaining the colloidal stability, preventing particle size growth and foaming.
Furthermore, once an aqueous suspension has been prepared, sedimentation of the suspension should be avoided. However, if sedimentation arises, the restoration of the suspension must be achieved as easily and as quickly as possible and advantageously this should be achieved by simply shaking the mixture by hand.
Failure to attain rapid reconstitution of the suspension results in an unacceptably high risk of a dosage error. Furthermore, rapid reconstitution is crucial given that the suspension is often dispensed using a dropper or a dispensing syringe, thereby requiring a stable liquid suspension which is able to flow freely from the dispensing devices and ensure that significantly less material sticks therein.
Additionally, foaming of the suspension formulation should be avoided, given that this also results in the dosage being imprecise and uncertain.
Finally, suspension formulations that are used for oral administration, particularly for pediatric use, must not have a bitter taste.
SUMMARY OF THE INVENTION
The present invention provides an oral powder for suspension (dry suspension) containing 4-trifluoromethyl-N-(3,3a,4,4a,5,5a,6,6a-octahydro-1,3-dioxo-4,6-ethenocycloprop[f]isoindol-2-(1H)-yl)-benzamide, commonly known as Tecovirimat or ST-246, granulated powder and simethicone, which is suitable for reconstitution.
The present invention also provides a process for making the dry suspension comprising mixing ST-246 powder with simethicone.
The present invention further provides an aqueous pharmaceutical suspension formulation comprising ST-246, simethicone and a suspending agent for oral administration.
Additionally, the present invention provides a process for making the aqueous pharmaceutical suspension formulation comprising dispersing the dry suspension and a suspending agent in water.
The present invention also provides methods of treating orthopoxvirus infections and/or eczema vaccinatum comprising administering to a subject in need thereof an aqueous pharmaceutical suspension formulation according to the present invention.
These and other objects, advantages, and features of the invention will become apparent to those persons skilled in the art upon reading the details of the methods and formulations as more fully described below.



DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the % ST-246 release from formulations DS 54, 55, 57 and 58.
FIG. 2 shows the % ST-246 release from formulations DS 75, 77, 83 and 84.
FIG. 3 shows the dissolution of ST-246 from formulations DS 85, 86, and 87.
FIG. 4 shows the % ST-246 release from formulation DS 88 at different blending times.
FIG. 5 shows the % ST-246 release from DS 88 at different lubrication times.
FIG. 6 shows the % ST-246 release from DS 89 and 90.
FIG. 7 shows the % ST-246 release from DS 91 and 92.
FIG. 8A shows an observation that when passing undiluted simethicone through #20 screen the simethicone granules stick on the screen. FIG. 8B shows an observation when passing undiluted simethicone granules through #20 screen the simethicone granules stick on the screen. FIG. 8C shown an observation that when passing pre-diluted simethicone through #20 screen the simethicone granules passed through the screen easily without sticking. FIG. 8D shows an observation that when passing co-milled simethicone-Tecovirimat granules through #20 screen the co-milled simethicone-Tecovirimat granules passed easily through the screen without sticking.
FIG. 9 shows retained clumps on #20 screen after sieve shaking of 100 g DS-92.
FIG. 10 shows % ST-246 release from DS 93 and 94.
FIG. 11 shows flavor profile definitions, including amplitude, amplitude scale, character notes, intensity scale and aftertaste.
FIG. 12 shows a time intensity profile of musty aromatic off-notes, bitterness and tannin mouthfeel of the Tecovirimat granules lingered at patient-perceptible levels (≥1) for about 3 minutes in the aftertaste.
FIG. 13 shows a time intensity profile of Tecovirimat formulation #4 (micronized API).
FIG. 14 shows a time intensity profile of formulation #1 (strawberry)
FIG. 15 shows a time intensity profile of formulation #2 (cherry)



DETAILED DESCRIPTION OF THE INVENTION
Tecovirimat (ST-246) is an antiviral agent used for smallpox and related orthopoxviruses. A suitable oral formulation, according to the present invention, is particularly useful in pediatric and/or geriatric populations. The typical maximum dosage is 1200 mg/day. The drug has very low water solubility over the physiologically relevant pH range, namely approximately 2 μg/mL over a pH range of 1.2 to 6.5. Its solubility increases slightly above pH 6.5, however the chemical stability declines. The solution stability is optimum in the pH 4 to 7 range, for example the pH 3 to 5 range.
Due to low solubility, preferably the ST-246 is micronized in order to improve dissolution rate. ST-246 has the potential to undergo supersaturation and crystallization with increased particle size in an aqueous based vehicle, and hence, providing an acceptable ready-to-use liquid/suspension dosage form with better stability and longer shelf-life represents a major challenge.
Additionally, when forming a suspension formulation containing ST-246, a wetting agent, such as a surfactant, is typically used to improve wetting of ST-246 particles, and also to improve dissolution and solubility. However, the presence of a surfactant usually leads to foaming and results in the formulation tasting bitter and having an unpleasant odor.
The present invention provides a powder for a reconstitution product (dry suspension) comprising ST-246 granulated powder and simethicone which when suspended in water exhibits acceptable stability and dissolution. Furthermore it has been found that the use of simethicone with ST-246 provides excellent foam control upon reconstitution.
The present invention provides a similar drug release profile, a critical product attribute for product performance in patients, as compared to capsule oral dosage form of ST-246.
Additionally, the incorporation of additional excipients, flavorings and/or sweeteners to the dry suspension and/or the aqueous pharmaceutical suspension formulation improves the taste and texture of the formulation.
The dry suspension and/or the suspension formulation may also include one or more pharmaceutically acceptable ingredients selected from the group consisting of disintegrants, carriers, diluents, additives, fillers, lubricants, and binders.
Disintegrants may include agar-agar, alginic acid, calcium carbonate, microcrystalline cellulose, croscarmellose sodium, crospovidone, polacrilin potassium, sodium starch glycolate, potato or tapioca starch, pre-gelatinized starch, other starches, clays, other algins, other celluloses, gums, and mixtures thereof.
Preferably, the dry suspension further comprises at least one suspending agent.
The suspending agents may include sorbitol syrup; sugar syrup; synthetic polymers such as carbomer and poloxamer; natural gums such as agar, xanthan gum, and tragacanth; cellulose derivatives (e.g., carboxy methyl cellulose, hydroxyl propyl cellulose or methylcellulose); or hydrogenated edible fiats; emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid).
The dry suspensions can also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.
Furthermore, the dry suspensions may also contain wetting agents and/or surface active agents as appropriate.
Preferably, the wetting agent or surface active agent is sodium dodecyl sulfate, sodium docusate, tweens, spans, brij, or cetrimide.
Preferably, the suspending agent is methylcellulose and/or hydroxypropyl cellulose, and, advantageously, the dry suspension contains both methylcellulose and hydroxypropyl cellulose.
Preferably, the dry suspension contains methylcellulose 400 cps and/or methycellulose 15 cps and, advantageously, the dry suspension contains both methylcellulose 400 cps and methycellulose 15 cps methylcellulose.
Typically, the dry suspension comprises a lubricant, such as calcium stearate, magnesium stearate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc, hydrogenated vegetable oil (e.g., peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, corn oil, and soybean oil), zinc stearate, ethyl oleate, ethyl laureate, agar, and mixtures thereof.
Advantageously, the lubricant is magnesium stearate.
Excipients include, but are not limited to, agents used to improve taste, suspendability, texture, and flavor. In one embodiment, when the dry suspension contains an excipient, the excipient is advantageously lactose monohydrate. When present, the total amount of excipients are typically present in an amount of about 10 wt % to about 20 wt %.
The dry suspension may also contain a further antifoaming agent.
Preferably, the dry suspension also contains a sweetener, such as sucralose.
Advantageously, the dry suspension contains a flavoring such as strawberry or black cherry.
The ST-246 may be selected from the group consisting of ST-246 polymorph Form I, ST-246 polymorph Form II, ST-246 polymorph Form III, ST-246 polymorph Form IV, ST-246 polymorph Form V and ST-246 polymorph Form VI.
The ST-246 may be micronized and/or granulated with excipients, but is advantageously micronized and granulated powder.
Furthermore the simethicone may be in granular form or may be in liquid form and adsorbed on lactose monohydrate. Advantageously, the simethicone is in granular form.
The dry suspension typically contains between 10 to 70 wt % of ST-246, and preferably between 15 to 40 wt % of ST-246.
The dry suspension usually contains between 0.2 to 6.0 wt % simethicone, and preferably between 0.4 to 5.0 wt % simethicone.
Preferably, the dry suspension contains between 1 to 5 wt % of methylcellulose, and advantageously, between 2 to 3 wt % of methylcellulose.
Preferably, the dry suspension contains between 1.0 to 30 wt % of hydroxypropylcellulose, and, advantageously, between 2 to 20 wt % of hydroxypropylcellulose.
When the dry suspension contains a lubricant, typically the lubricant is present in the amount 0.1 to 1.0 wt %.
Similarly, when the dry suspension contains a sweetener, typically the sweetener is present in the amount of between 1.0 to 3.0 wt %, and when the dry suspension contains a flavoring, typically the flavoring is also present in the amount of between 0.1 to 1.0 wt %.
The dry suspension preferably has an active pharmaceutical ingredient (API) particle size of ST-246 of between 0.5 to 20 μm, or between 0.5 to 10 μm, or between 1 μm and 5 μm, and, advantageously, between 1 to 10 μm.
It has been found that the higher the API particle size, the slower the dissolution rate.
The present invention also provides an aqueous pharmaceutical suspension formulation comprising the dry suspension dispersed in water.
Typically the suspension, either dry or liquid, contains a suspending agent which is preferably selected from the group consisting of hydromellose, hydroxyethylcellulose, carrageenans, methylcellulose, xanthan gum, povidone, hydroxypropyl cellulose, microcrystalline cellulose, carboxymethylcellulose and sodium alginate.
When the suspension contains water and a suspending agent this is typically referred to as a ‘formulation vehicle’.
Typically, the formulation, upon reconstitution with water, has a pII between 3 and 8, preferably between 5 and 7.
The present invention also provides a method of treating orthopoxvirus infections and/or eczema vaccinatum comprising oral administration to a subject in need thereof the above mentioned formulation. Typically, the formulation of the present invention is administered in an amount such that the dose of ST-246 is 200 mg to 2000 mg per day, preferably 400 mg to 2000 mg per day, and most preferably 600 mg to 1200 mg daily. Dosage is typically adjusted according to body weight, particularly in pediatric populations.
Additionally, the present invention also provides a process of making the dry suspension which involves mixing ST-246 or ST-246 granulated powder with simethicone.
Optionally, at least one suspending agent, at least one lubricant, at least one excipient, at least one further anti-foaming agent, at least one sweetener and/or at least one flavoring may be mixed with the ST-246 and the simethicone.
Advantageously, mixing is carried out using geometric mixing.
Finally, the present invention provides a method of making the above mentioned formulation wherein the dry suspension is dispersed in water, and wherein the water advantageously contains a suspending agent as herein described above.
As used herein. “percent,” “percentage” or the symbol “%” means the percent of the component indicated in the composition based on the amount of the carrier present in the composition, on a weight/weight (w/w), weight/volume (w/v) or volume/volume (v/v) concentration, as indicated with respect to any particular component, all based on the amount of the carrier present in the composition. Thus, different types of carriers can be present in an amount of up to 100% as indicated, which does not preclude the presence of the API, the amount of which can be indicated as a % or as a certain number of mg present in the composition or a certain number of mg/mL present, where the % or mg/mL is based on the amount of the total carrier present in the composition. Certain types of carriers can be present in combination to make up 100% of the carrier.
As used herein, the terms “Tecovirimat” and “ST-246” refer to the compound 4-trifluoromethyl-N-(3,3a,4,4a,5,5a,6,6a-octahydro-1,3-dioxo-4,6-ethenocycloprop[f]isoindol-2-(1H)-yl)-benzamide.
The invention is further described by the examples given below.
EXAMPLES
Example 1. Production of a Dry Suspension Containing Tecrovirmat Monohydrate (ST-246) for Reconstitution, 200 mg
A stable oral powder containing ST-246 was prepared, particularly for pediatric and % or geriatric application. The development of the powder involved the following stages;

    
    
        1. Formulation and evaluation of various dry suspensions.
        2. Optimization and evaluation of various level of anti-foaming agents and lubricant and their effect on product properties.
        3. Optimization and evaluation of blending time and lubrication time in the product manufacturing process and its effect on product properties.
        4. Manufacture of batches for stability studies.

Materials: The Following Materials were Used.

    
    














S. #
.Material
Supplier/Lot#

















1
Tecovirimat Monohydrate, micronized, 
Siga Technologies



for discriminatory dissolution method 
Lot # SG-10C12-T1039



development
(Trial #2, d90 = 21.51 




μm)


2
Tecovirimat Monohydrate, micronized, 
Siga Technologies



for discriminatory dissolution method 
Lot # SG-10C12-T1039



development
(Trial #3, d90 = 38.74 




μm)


3
Tecovirimat Monohydrate, micronized, 
Siga Technologies



for discriminatory dissolution method 
Lot# SG-10C12-T1039



development
(Trial #4, d90 = 81.53 




μm)


4
Tecovirimat Granulate
Siga Technologies




Lot # 1302679


5
Tecovirimat Granulate
Siga Technologies




Lot # 1401165


6
Colloidal silicon dioxide, NF
Cabot



(Cabosil MSP)
Lot #1222272


7
Croscarmellose sodium, NF
FMC Biopolymer



(AcDiSol)
Lot # TN12824921


8
Lactose monohydrate, NF
DFE



(SuperTab11SD)
Lot # 10705838


9
Lactose monohydrate, NF
DFE



(SuperTab11SD)
Lot # 10700181


10
Lactose monohydrate, NF
DFE



(SuperTab11SD)
Lot # 10737298


11
Microcrystalline cellulose, NF
FMC Biopolymer



(Avicel PII101)
Lot # P113825526


12
Ilypromellose, USP
Dow



(Methocel E3 LV Premium)
Lot # 1D020124L1


13
Sodium lauryl sulfate, NF
Spectrum




Lot # 1DB0596


14
Purified Water, USP
Ricca Chemicals




Lot # 4409016


15
Sucralose, NF
Spectrum




Lot # 1CF0697


16
Sucralose, NF
Spectrum




Lot # 1DF0586


17
Strawberry flavor, #133.16296
Bell Flavors


18
Black cherry nat type flavor
Kerry




Lot # 260314


19
Simethicone, USP 
Nusil Technologies



(MED-340, simethicone content 100%)
Lot # 63865


20
Simethicone Granular Solid, USP
Nusil Technologies



(MED-342, simethicone content 30'%)
Lot # 63775


21
Simethicone Granular Solid, USP
Nusil Technologies



(MED-342, simethicone content 30%)
Lot # 66777


27
Simethicone Granular Solid, USP
Nusil Technologies



(MED-342, simethicone content 30%)
Lot # 67719


23
Magnesium stearate, NF
Mallinckrodt



(HyQual)
Lot # 1301000109


24
Crospovidone, USP/NF
BASF



(Kollidon CL-M)
Lot # 10204988Q0


25
Povidone, USP/NF
BASF



(Kollidon 12PF)
Lot # 12714424U0


26
Povidone, USP/NF
BASF



(Koi Udon, 90F)
Lot # 45877768E0


27
Hypromellose
Dow



(Methocel F50)
Lot # 2L03012N21


28
Hypromellose
Dow



(Methocel E50)
Lot # 2C01012N23


29
Methylcellulose, USP
Dow



(Methocel A4M Premium)
Lot # 2E19012N11


30
Methylcellulose, 400 cps, USP
Spectrum




Lot # 2CJ0081


31
Methylcellulose, 400 cps, USP
Spectrum




Lot # 2DC0205


32
Methylcellulose, 15 cps, USP
Spectrum




Lot # 2DE0263


33
Hydroxypropyl cellulose, NF
Ashland



(Klucel JF Pharm)
Lot # 38296


34
Hydroxypropyl cellulose, NF
Ashland



(Klucel JXF Pharm)
Lot # 40289


35
Hydroxypropyl cellulose, NF
Ashland



(Klucel HF Pharm)
Lot # 49264


36
Hydroxypropyl cellulose, NF
Ashland



(Khtcel MF Pharnt)
Lot # 38949


37
Hydroxylethyl cellulose, NF
Ashland



(Natrosol 250L Pharm)
Lot # J1967


38
Carrageenan, NF
FMC Biopolymer



(Gelcarin GP3 79)
Lot # 50218031


39
Carrageenan, NF
FMC Biopolymer



(SeaSpen PF)
Lot # 20226021


40
Microcrystalline Cellulose and Sodium
FMC Biopolymer



Carboxymethylcellulose, NF
Lot # B1204C



(Avicel RC-581)



41
Microcrystalline Cellulose and Sodium
FMC Biopolymer



Carboxymethylcellulose, NF
Lot # EN13825521



(Avicel CL-611)



42
Microcrystalline Cellulose and Sodium
FMC Biopolymer



Carboxymethylcellulose, NF
Lot # DN13825459



(Avicel RC-591)



43
Sodium Alginate, USP/NF
FMC Biopolymer



(Protanal LFR5/60)
Lot # H191208


44
Xanthan gum, USP/NF
CP Kelco



(Xantural 75)
Lot # 2B4685K


45
Simethicone 50% Powder
AIC




Lot # 4060-50205









Two typical dry suspensions are shown in Table. The dry suspensions according to the present invention contain granules of ST-246 and additional excipients to improve taste suspendability, texture and favor.










TABLE 1







Compo- 
Compo- 




sition of
sition of




Formulation
Formulation


No.
Ingredient
DS83 (mg)
DS84 (mg)


















1
Tecovirimat granulate
346.5
346.5


2
Lactose monohydrate (SuperTab 
93.0
0.0



11SD, DMV Fonterra Excipients)




3
Klucel JF Pharm (Ashland)
125.0
118.0


4
Sucralose, USP
10.0
10.0


5
Flavor, black cherry (Virginia Dare, 
3.0
3.0



#23950)




6
Simethicone granular solid (NuSil 
20.0
20.0



MED-342)




7
Magnesium stearate
2.5
2.5



Total
600.0
500.0









Example 2: Development of the Dry Suspensions for Reconstitution, 200 mg
The composition of the Tecovirimat granulate is shown in Table 2. An oral powder for suspension using a suspending agent containing microcrystalline cellulose and sodium carboxymethylcellulose (Avicel CL-611) was used as a Target formulation is shown in Table 3. The target formulation exhibits good physical properties. However, the xanthan gum which is co-processed along with microcrystalline cellulose results in coagulation behavior during the dissolution tests due to the interaction with the surfactant used in the dissolution medium and thus poor drug release.







TABLE 2







Composition of ST-246 commercial granules











Quantity per 




Unit


S. No
Ingredients
(mg)












7
ST-246, Monohydrate, micronized
209.00



Microcrystalline Cellulose, NF (Avicel PH101)
49.628


3
Lactose Monohydrate, NF (Supertab 11SD)
33.15


4
Croscarmellose Sodium. NF (Ac-di-sol SD-711)
31.2


5
Colloidal Silicone Dioxide, NF (Cab-O-Sil ® 
1.95



M5P)



6
Hypromellose, USP (Methocel E3)
13.65


7
Sodium Lauryl Sulfate, NF
7.80


8
Purified Water*, USP
q.s.



Total
346.4





*water is removed during drying













TABLE 3







ST-246 oral powder for suspension (Target Formulation)










Prototype 1
Prototype 2



(mg per 
(mg per 


Ingredient
dose)
dose)












ST-246, granulate
346.5
346.5


Lactose monohydrate (SuperTab 11SD, DMV 
503.3 
505.1


Fonterra Excipients)




Simethicone granular solid, (NuSil MED-342)
10.0
10.0


Aviccl CL-611, NF (FMC BioPolymer)
125.0
125.0


Sucralose, USP
10.0
10.0


Flavor, strawberry (Kerry Item: U1-721636)
5.0
—


Flavor, black cherry (Kerry Item: U1-717664)
—
3.0


FD&C Red #3
0.10
—


FD&C red #40
55
0.3


Methylparaben, NF
0.08
0.08


Propylparaben, NF
0.02
0.02


Total
1,000.0
1,000.0









Example 3: Screening and Selection of Suspending Agent
ST-246 granules prepared for capsule dosage form were used for the preparation of ST-246 oral powder for reconstitution, 200 mg. To evaluate the suspendability of the granules in various suspending agents, various suspending agents were dispersed in 50 mL purified water (Table 4). Concentrations of suspending agents in formulations DS-1 to DS-16 were produced according to the maximum potential from FDA Inactive Ingredient Guide (IIG). A placebo was prepared and compared visually with the target formulation and the concentration of suspending agent was adjusted accordingly in formulation DS-17 to DS-38







TABLE 4







Composition and Observation of Suspending Agent Placebo Solutions/Suspensions.






















Consistency










(Visual










observation










in






Polymer



comparison
Viscosity





quantity



with target
(cps, target





equilvalent to



formulation
formulation



Brand
Formulation
10 doses

Disperse
Overnight
viscosity
viscosity


Chemicals
name
ID
(concentration)
Appearance
speed
sedimentation
+++++)
50-100 cps)


















Crospovidone
Kollidon
DS-1
2.500 g
white
fast
sedimentation,
less viscous
not



CL-M

(5%, w/v)
suspension

easy to
++
measured








redisperse by










shaking




Povidone (PVP)
Kollidon
DS-2
0.750 g
colorless
fast
none
less viscous
not



90 F

(1.5 %, w/v)
solution


+
measured




DS-17
1.500 g
colorless
fast
none
less viscous
not





(3%, w/v)
solution


+
measured




DS-29
3.000 g
colorless
fast
none
comparable
55.8





(6%, w/v)
solution


++++




Kollidon
DS-3
0.750 g
colorless
fast
none
less viscous
not



12 PF

(1.5%, w/v)
solution


+
measured




DS-18
1.500 g
colorless
fast
none
less viscous
not





(3%, w/v)
solution


+
measured




DS-30
3.000 g
colorless
fast
none
less viscous
not





(6%, w/v)
solution


++
measured


Hypromellose
Methocel
DS-4
1.500 g
colorless
slow
none
comparable
43.1


(HPMC)
F50

(3%, w/v)
solution


+++++




Methocel
DS-5
1.500 g
colorless
slow
none
comparable
85.6



E50

(3%, w/v)
solution


+++++





DS-19
0.750 g
colorless
slow
none
less viscous
not





(1.5%, w/v)
solution


++
measured


Methylcellulose
Methocel
DS-6
0.595 g
colorless
slow
none
more
not


(MC)
A4MP

(1.19%, w/v)
solution


viscous
measured









++++++





DS-26
0.250 g
colorless
slow
none
comparable
24.9





(0.5%, w/v)
solution


++++




Methyl
DS-7
0.595 g
colorless
slow
none
comparable
not



cellulose,

(1.19%, w/v)
solution


+++++
measured



400 cps
DS-27
0.250 g
colorless
slow
none
comparable
17.1





(0.5%, w/v)
solution


++++



Hydroxypropyl
Klucel
DS-8
3.350 g
colorless
slow
none
more
not


cellulose (HPC)
JF Pharm

(6.7%, w/v)
solution


viscous
measured









+++++++





DS-20
0.750 g
colorless
slow
none
less viscous
not





(1.5%, w/v)
solution


++
measured




DS-31
1.500 g
colorless
slow
none
comparable
20.4





(3%, w/v)
solution


++++




Klucel
DS-9
3.350 g
colorless
slow
none
more
not



HF

(6.7%, w/v)
gel


viscous, gel,
measured



Pharm





high










viscosity





DS-21
0.500 g
colorless
slow
none
more
not





(1.0%, w/v)
solution


viscous
measured









++++++++





DS-33
0.375 g
colorless
slow
none
comparable
123.9 





(0.75%, w/v)
solution


++++++




Klucel
DS-10
3.350 g
colorless
slow
none
more
not



MF

(6.7%, w/v)
gel


viscous, gel,
measured



Pharm





high










viscosity





DS-22
0.500 g
colorless
slow
none
more
not





(1.0%, w/v)
solution


viscous
measured









+++++++





DS-34
0.375 g
colorless
slow
none
comparable
71.0





(0.75%, w/v)
soluton


+++++



Hydroxyethyl
Natrosol
DS-11
1.500 g
yellowish
fast
none
comparable
14.9


cellulose (HEC)
250L

(3%, w/v)
solution


++++




Pharm
DS-28
2.000 g
yellowish
fast
none
comparable
not





(4%, w/v)
solution


++++
measured


Carrageenan
Gelcarin
DS-12
0.750 g
yellowish
slow
none
more
not



GP379

(1.5%, w/v)
gel


viscous, gel,
measured









high










viscosity





DS-23
0.200 g
yellowish
slow
none
comparable
65.5





(0.4%, w/v)
solution


+++++




SeaSpen
DS-13
0.750 g
milky
slow
none
more
not



PF

(1.5%, w/v)
gel


viscous, gel,
measured









high










viscosity





DS-24
0.200 g
milky
slow
none
comparable
20.1





(0.4%, w/v)
solution


++++++



Microcrystalline
Avicel
DS-14
1.500 g
white
fast
none
more
not


cellulose/sodium
RC-581

(3%, w/v)
suspension


viscous
measured


carboxymethyl






+++++++



cellulose

DS-35
1.250 g
white
fast
none
comparable
84.3


(MCC/CMCNa)


(2.5%, w/v)
suspension


++++++




Avicel
DS-15
1.500 g
white
fast
none
more
not



CL-611

(3%, w/v)
suspension


viscous
measured









+++++++





DS-36
1.250 g
white
fast
none
comparable
35.4





(2.5%, w/v)
suspension


+++++




Avicel
DS-37
1.250 g
white
fast
none
comparable
93.8



RC-591

(2.5%, w/v) 
suspension


++++++



Sodium
Protanal
DS-16
0.062 g
colorless
fast
none
less viscous
not


Alginate
LFR

(0.123%, w/v)
solution


+
measured



5/60
DS-25
0.800 g
yellowish
slow
none
less viscous
not





(1.6%, w/v)
solution


++
measured




DS-32
1.600 g
yellowish
slow
none
less viscous
not





(3.2%, w/v)
solution


++++
measured




DS-38
2.000 g
yellowish
slow
none
comparable
32.4





(4%, w/v)
solution


+++++









Povidone (Kollidon 90 F) 6%, Hypromellose (Methocel F 50 and E50) 3%, Methylcellulose (Methocel A4MP), Methyl Cellulose 400 cps 0.5%, Hydroxypropyl cellulose (Klucel HF and MF) 0.75%, Hydroxy Ethyl Cellulose (Natrosol 250 L Pharm) 3%, Avicel RC-581—2.5%. Avicel RC-591—2.5% and Sodium alginate 4% show comparable viscosity to the Target formulation containing Avicel CL-611.
The suspending agent concentrations with comparable consistency to that of the Target formulation (containing Avicel CL-611 as suspending agent) were selected as the formulation viscosity modifier.
Example 4: Formulation of Suspensions by Mixing ST-246 Granules with Formulation Vehicles
To evaluate the suspendability of ST-246, ingredients such as sucralose, simethicone (adsorbed on lactose monohydrate) and suspension polymers were added. Lactose monohydrate was added to make up the weight of the composition. These ingredients were mixed with the selected suspending agent vehicle concentration from Example 3. The resultant compositions are given in Table 5.
The formulation procedure included:

    
    
        1. Adsorbing 1 g of simethicone (MED-340, liquid) on 19 g lactose monohydrate (SuperTab 11SD).
        2. Geometric mixing of the ST-246 granules, sucralose, simethicone adsorbed on lactose, and lactose monohydrate to make 5 g (5 doses) blends considering the quantity of polymer quantity sufficient (Q.S.) to 25 mL of vehicle.
        3. Dispersing the blend in Q.S. to 25 mL of formulation vehicle prepared in Example 3.
        4. Evaluation of the sedimentation, redispersibility, and pH of the suspensions.
        5. Transfer of each of about 5 mL of suspension in screw cap glass vials, to store the samples at room temperature, at 2 to 8° C. and at 40° C./75% RH for 14 Days and recording the observations. Table 6 shows the results of physical properties of suspensions.
    
    









TABLE 5





Composition of suspensions-Suspending Agent Selection



















Quantity (g) Equivalent to 5 doses
















S.#
Ingredients
DS-39
DS-40
DS-41
DS-42
DS-43
DS-44
DS-45
DS-46





1
ST-246 Granules
1.733
1.733
1.733
1.733
1.733
1.733
1.733
1.733


2
Sucralose, NF
0.050
0.050
0.050
0.050
0.050
0.050
0.050
0.050


3
Simethicone, USP (MED-340)
0.025
0.025
0.025
0.025
0.025
0.025
0.025
0.025



Lactose Monohydrate, NF,
0.475
0.475
0.475
0.475
0.475
0.475
0.475
0.475



(SuperTab 11SD)










5
Lactose Monohydrate, NF
1.967
1967
1.967
2.617
2.617
2.592
2.592
1.217



(SuperTab 11SD)










6
Hypromellose (Methocel F 50), 
25.750
—
—
—
—
—
—
—



3% Dispersion











Hypromellose (Methocel E 50),
—
25.750
—
—
—
—
—
—



3% Dispersion











Hydroxyethyl cellulose 
—
—
25.750
—
—
—
—
—



(Natrosol 250L), 3% Dispersion











Carrageenan (Iota) (Gelcarin GP379),
—
—
—
25.100
—
—
—
—



0.4% Dispersion











Carrageenan (Iota) (SeaSpen PF),
—
—
—
—
25.100
—
—
—



0.4% Dispersion











Methyl Cellulose (Methocel A4MP),
—
—
—
—
—
25.125
—
—



0.5% Dispersion











Methyl Cellulose, 400 cps, 0.5% Dispersion
—
—
—
—
—
—
25.125
—



Povidone (Kollidon 90F), 6% Dispersion
—
—
—
—
—
—
—
26.500



Hydroxypropyl cellulose (Klucel JF Pharm), 
—
—
—
—
—
—
—
—



3% Dispersion











Hydroxypropyl cellulose (Klucel HF Pharm), 
—
—
—
—
—
—
—
—



0.75% Dispersion











Hydroxypropyl cellulose (Klucel MF Pharm), 
—
—
—
—
—
—
—
—



0.75% Dispersion











Microcrystalline cellulose and carboxymethyl 
—
—
—
—
—
—
—
—



cellulose (Avicel RC-581), 2.5% Dispersion











Microcrystalline cellulose and carboxymethyl 
—
—
—
—
—
—
—
—



cellulose (Avicel CL-611), 2.5% Dispersion











Microcrystalline cellulose and carboxymethyl 
—
—
—
—
—
—
—
—



cellulose (Avicel RC-591), 2.5% Dispersion











Sodium Alginate (Protanal LFR 5/60), 
—
—
—
—
—
—
—
—



4% Dispersion


















S.#
Ingredients
DS-47
DS-48
DS-49
DS-50
DS-51
DS-52
DS-53





1
ST-246 Granules
1.733
1.733
1.733
1.733
1.733
1.733
1.733


2
Sucralose, NF
0.050
0.050
0.050
0.050
0.050
0.050
0.050


3
Simethicone, USP (MED-340)
0.025
0.025
0.025
0.625
0.025
0.025
0.025



Lactose Monohydrate, NF,
0.475
0.475
0.475
0.475
0.475
0.475
0.473



(SuperTab 11SD)









5
Lactose Monohydrate, NF
1.967
2.530
2.530
2.092
2.092
2.092
1.717



(SuperTab 11SD)









6
Hypromellose (Methocel F 50), 
—
—
—
—
—
—
—



3% Dispersion










Hypromellose (Methocel E 50),
—
—
—
—
—
—
—



3% Dispersion










Hydroxyethyl cellulose 
—
—
—
—
—
—
—



(Natrosol 250L), 3% Dispersion










Carrageenan (Iota) (Gelcarin GP379),
—
—
—
—
—
—
—



0.4% Dispersion










Carrageenan (Iota) (SeaSpen PF),
—
—
—
—
—
—
—



0.4% Dispersion










Methyl Cellulose (Methocel A4MP),
—
—
—
—
—
—
—



0.5% Dispersion










Methyl Cellulose, 400 cps, 0.5% Dispersion
—
—
—
—
—
—
—



Povidone (Kollidon 90F), 6% Dispersion
—
—
—
—
—
—




Hydroxypropyl cellulose (Klucel JF Pharm), 
25.750
—
—
—
—
—
—



3% Dispersion










Hydroxypropyl cellulose (Klucel HF Pharm), 
—
25.187
—
—
—
—
—



0.75% Dispersion










Hydroxypropyl cellulose (Klucel MF Pharm), 
—
—
25.187
—
—
—
—



0.75% Dispersion










Microcrystalline cellulose and carboxymethyl 
—
—
—
25.625
—
—
—



cellulose (Avicel RC-581), 2.5% Dispersion










Microcrystalline cellulose and carboxymethyl 
—
—
—
—
25.625
—
—



cellulose (Avicel CL-611), 2.5% Dispersion










Microcrystalline cellulose and carboxymethyl 
—
—
—
—
—
25.625
—



cellulose (Avicel RC-591), 2.5% Dispersion










Sodium Alginate (Protanal LFR 5/60), 
—
—
—
—
—
—
26.000



4% Dispersion
















The sedimentation coefficients were determined by measuring the height of the suspension in a 40 cc vial divided by the height of the sediment in a vial. Sedimentation coefficient versus time is used as an indicator as to the stability of suspension over the time.
The redispersibility was evaluated by centrifuging the suspension in Eppendorf tube at 5000 rpm for 10 minutes followed by shaking manually or with wrist action shaker, if the sediment in centrifuge suspended again, it was recorded as “redispersible.”







TABLE 6





Characterization of Physical Stability of Selected Formulations
























DS-39
DS-40
DS-41
DS-42
D5-43
DS-44
DS-45
DS-46



Methocel
Methocel
Natrosol
Gelcarin
SeaSpen
Methocel
MC,
Kollidon



F50
E50
250L
GP379
PF
A4MP
400 cps
90F


Formulation ID
3%
3%
3%
0.4%
0.4%
0.5%
0.5%
6%


Polymer
w/v
w/v
w/v
w/v
w/v
w/v
w/v
w/v










Observations
















1
Appearance
White
White
White
White
White
White
White
White




dispersion
dispersion
dispersion
dispersion
dispersion
dispersion
dispersion
dispersion


2
Sedimentation
100
100
1.00
1.00
1.00
1.00
1.00
1.00



Coeff (10 min)










3
Sedimentation
1.00
1.00
1.00
1 00
1.00
1.00
1.00
1.00



Coeff (30 min)










4
Sedimentation
1.00
1.00
0.72
0.46
1.00
1.00
1.00
0.51



Coeff (12 h)










5
Sedimentation
100
1.00
0.58
0.38
1.00
1.00
0.92
0.41



Coeff (24 h)










6
Observation
None
None
None
None
None
Noite
None
None



at the time 











of mixing 











the solids 











with the











formulation











vehicle










7
Redispersibility
No
No
Redispersible
Redispersible
No
No
Redispersible
Redispersible



after 24 hours 
sedimentation
sedimentation


sedimentation
sedimentation





(gentle shaking)










8
pH
5.62
5.81
5.57
5.30
6.67
5.71
5.78
5.67







Stability: 2-8° C. for 14 days
















1
Appearance
white
white
white
white
white 
white
white
white



(Color)










2
Signs of
two
two
almost
clear
uni-
two
two
clear



Creaming,
layers
layers
clear
supernatant
form
layers
layers
supernatant



flocculation


supernatant







3
Sedimentation
0.98
0.99
0.34
0.34
0.97
0.97
0.95
0.31



Coeff










4
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible







Stability: 40° C./75% RH for 14 days
















1
Appearance
white
white
white
white
white
white
white
white



(Color)










2
Signs of
two
two
clear
clear
clear
clear
clear
clear



Creaming,
layers
layers
supernatant
supernatant
supernatant
supernatant
supernatant
supernatant



flocculation





sedimentation
sedimentation










two
two










layers
layers



3
Sedimentation
0.97
0.98
0.34
0 37
0.34
0.52
0.56
0.34



Coeff










4
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible







Stability: Room temperature for 14 days
















1
Appearance
white
white
white
white
white
white
while
white



(Color)










2
Signs of
two
two
clear
clear
clear
two
two
opaque



Creaming,
layers
layers
supernatant
supernatant
supernatant
layers
layers
supernatant



flocculation











Sedimentation
0.98
0.97
0.30
0.32
0.76 
0.65
0.94
0.30



Coeff










4
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible


5
pH
5.62
5.58
5.49
5.25
6.77
5.65
5.66
5.44




















DS-47
DS-48
DS.49
DS-50
DS-51
DS-52
DS-53




Klucel
Klucel
Klucel
Avicel 
Avicel
Avicel 
Protanal














Formulation ID
JF
HF
MF
RC-581
CL-611
RC-591
LFR5/60


Polymer
3% w/v
0.75% w/v
0.75% w/v
2.5% w/v
2.5% w/v
2.5% w/v
4% w/v










Observations















1
Appearance
White
White
White
White
White
White
White




dispersion
dispersion
dispersion
dispersion
dispersion
dispersion
dispersion


2
Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00
1.00



Coeff (10 min)









3
Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00
1.00



Coeff (30 min)









4
Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00
0.27



Coeff (12 h)









5
Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00
0.23



Coeff (24 h)









6
Observation
Thickening
Thickening 
Thickening
None
None
None
Thinning of 



at the time 
of
of
of



formulation



of mixing 
formulation
formulation,
formulation,



after



the solids

gel
gel



overnight



with the 






standing



formulation










vehicle









7
Redispersibility
No
No
No
No
No
No
Redispersible



after 24 hours 
sedimentation
sedimentation
sedimentation
sedimentation
sedimentation
sedimentation
but



(gentle shaking)






agglomerates


8
pH
5.76
Not
Not
5.58
5.56
5.57
5.16





checked
checked











Stability: 2-8° C. for 14 days















1
Appearance
white
Not
Not
while
white
white
white



(Color)

checked
checked






2
Signs of
two layers


creamy, 
uniform
creamy
clear



Creaming,



phase


supernatant



flocculation



separation





3
Sedimentation
0.98


0.83
1.00
1.00
0.21



Coeff









4
Redispersibility
redispersible


redispersible
redispersible
redispersible
redispersible







Stability: 40° C./75% RH for 14 days















1
Appearance
white
Not
Not
white
white
white
white



(Color)

checked
checked






2
Signs of
a thin


creamy,
uniform
creamy
clear



Creaming,
clear


phase


supernatant



flocculation
supernatant,


separation







a thick










flocculation










layer








3
Sedimentation
0.59


0.78
1.00
1.00
0.34



Coeff









4
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible







Stability: Room temperature for 14 days















1
Appearance
white
white
white
white
white
white
white



(Color)









2
Signs of
opaque
very
very
creamy,
uniform
uniform
clear



Creaming,
supernatant
viscous gel
viscous gel
phase


supernatant



flocculation



separation





3
Sedimentation
0.42
1.00
1.00
0.84
1.00
1.00
0.33



Coeff









4
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible
redispersible


5
pH
5.49
4.74
5.65
5.53
5.49
5.50
5.14









Suspensions were prepared using 3% Methocel F50, 3% Methocel E50, 0.5% Methocel A4MP, 0.5% Methylcellulose 400 cps, or 3% Klucel JF as suspending agents and showed comparable physical properties as the Target formulation using 2.5% Avicel CL-611 as a vehicle, and also showed comparable results of physical stability when stored at 2 to 8° C., room temperature and at 40° C./75% RH for the reconstitution study.
Example 5: Formulation and Evaluation of Powders for Reconstitution Using Selected Polymers
Solid blends (including suspending agents selected in Example 4) were formulated, and the rates of reconstitution (hydration time) in water were evaluated. Compositions are given in Table 7.







TABLE 7







Composition of solid blends (including suspending agents) 


for reconstitution (40 mg/ml).











Quantity per unit (mg)













S.#
Ingredients
DS-54
DS-55
DS-56
DS-57
DS-58
















1
ST-246 Granules*
346.50
346.50
346.50
346.50
346.50


2
Sucralose, NF
10.00
10.00
10.00
10.00
10.00


3
Simethicone, USP 
5.00
5.00
5.00
5.00
5.00



(MED-340)







4
Lactose
95.00
95.00
95.00
95.00
95.00



Monohydrate, NF








(SuperTab 11SD)







5
Lactose
393.50
393.50
518.50
518.50
393.50



Monohydrate, NF








(SuperTab 11SD)







6
Hypromellose 
150.00
—
—
—
—



(Methocel F50)








Hypromellose 
—
150.00
—
—
—



(Methocel E50)








Methyl Cellulose 
—
—
25.00
—
—



(Methocel 








A4MP), USP








Methyl Cellulose, 
—
—
—
25.00
—



400 cps, USP








Hydroxypropyl 
—
—
—
—
150.00



cellulose








(Klucel JF 








Pharm), NF







7
Purified water, 
—
—
—
—
—



USP, Q.S. to 5 mL

















Total
1000.00
1000.00
1000.00
1000.00
1000.00





*granules equivalent to 200 mg Tecovirimat






The formulation procedure was as follows;

    
    
        1. Adsorbing of 1 g of simethicone (MED-340, liquid) on 19 g lactose monohydrate (SuperTab 11SD)
        2. Geometric mixing of Tecovirimat granules, sucralose, and simethicone adsorbed lactose, then weighing of quantity equivalent to 10 units of blend and then geometric mixing with other components for each formulation;
        3. Dispersing of the blend in purified water to make Q.S. to 50 mL;
        4. Observing the rate of reconstitution, sedimentation, redispersibility, and pH of the suspensions (see Table 8);
        5. Storing the samples at room temperature, at 2 to 8° C. and at 40° C./75% RH for 14 Days and recording the observations (see Table 9);
        6. Conducting a freeze-thawing cycle of 3-day—10° C. and 3-day room temperature and repeating 5 times and recording the observations (see Table 10 and 11).
    
    









TABLE 8







Observation of reconstitution and 1-day sedimentation of DS-54 to DS-58













DS-54
DS-55
DS-56
DS-57
DS-58


Formulation ID
Methocel F50
Methocel E50
Methocel A4MP
MC, 400 cps
Klucel JF


Polymer
3% w/v
3% w/v
0.5% w/v
0.5% w/v
3% w/v










Observations T = 0, room temperature













1
Time for 
7
7
1
1
2



reconstitution








(min)







2
Observation for
Still chunks
Still chunks
No chunks
No chunks
Small chunks



reconstitution
remaining at
remaining at
remaining, 
remaining, 
remaining at




bottom after
bottom after
easy to 
easy to 
bottom after




shaking
shaking
disperse
disperse
shaking


3
Appearance (visual)
white, uniform
white, uniform
white, uniform
white, uniform
white, uniform


4
pH
5.76
5.81
5.86
5.89
5.78


5
Particle size (μm)
3.20 ± 2.56
3.03 ± 1.91
3.85 ± 3.11
3.3 1 ± 2.43
3.86 ± 3.31


6
Forced settling and
redispersible
redispersible
redispersible
redispersible
redispersible



Redispersibility












Observations T = 30 min, room temperature













1
Sedimentation Coefficient
1.00
1.00
1.00
1.00
1.00


2
Appearance (visual)
white, uniform
white, uniform
white, uniform
white, uniform
white, uniform


3
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible







Observations T = 1 hr, room temperature













1
Sedimentation Coefficient
1.00
1.00
1.00
1.00
1.00


2
Appearance (visual)
white, uniform
white, uniform
white, uniform
white, uniform
white, uniform


3
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible







Observations T = 24 hr, room temperature













1
Sedimentation Coefficient
1.00
1.00
1.00
0.98
0.95


2
Appearance (visual)
white, uniform
white, uniform
two layers
two layers
opaque








supernatant 








with








floccules


3
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible
















TABLE 9







14-Days stability of DS-54 to DS-58













DS-54
DS-55
DS-56
DS-57
DS-58


Formulation ID
Methocel F50
Methocel E50
Methocel A4MP
MC, 400 cps
Klucel JR


Polymer
3% w/v
3% w/v
0.5% w/v
0.5% w/v
3% w/v










Observations T = 7 days, room temperature













1 
Sedimentation 
0.95
0.97
0.95
0.96
0.65



Coefficient







2 
Appearance 
two layers
two layers
two layers
two layers
opaque 



(visual)




supernatant








with 








floccules


3 
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible


4 
pH
5.72
5.79
5.77
5.78
5.70


5 
Particle 
3.33 ± 2.67
3.37 ± 2.29
3.11 ± 1.88
3.37 ± 1.96
3.20 ± 2.13



size (μm)












Observations T = 7 days, 2-8° C.













1
Sedimentation 
0.97
0.97
0.98
0.99
0.87



Coefficient







2
Appearance 
two layers
two layers
two layers
two layers
opaque 



(visual)




supernatant








with 








floccules


3 
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible


4 
pH
5.75
5.82
5.81
5.79
5.75


5 
Particle
3.13 ± 2.18
3.20 ± 2.30
3.51 ± 2.37
3.37 ± 2.08
3.57 ± 2.52



size (μm)












Observations T = 7 days, 40° C./75% RH













l 
Sedimentation 
0.35
0.43
0.44
0.41
0.81



Coefficient







2 
Appearance 
milky 
opaque 
clear 
opaque 
opaque 



(visual)
supernatant
supernatant
supernatant
supernatant
supernatant






with 

with 






floccules

floccules


3 
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible


4 
pH
5.48
5.55
5.53
5.57
5.44


5 
Particle
3.51 ± 2.49
3.45 ± 2.54
3.86 ± 2.55
3.96 ± 3.10
3.81 ± 2.37



size (μm)












Observations T = 14 days, room temperature













1
Sedimentation 
0.96
0.96
0.49
0.96
0.69



Coefficient







2
Appearance 
two layers
two layers
two layers,
two layers
opaque 



(visual)


clear

supernatant






supernatant 

with 






with

floccules






floccules




3
Redispersibility
redispersible
redispersible
redispersible;
redispersible
redispersible






sedimentation








occurs 








at 30 min








after shaking




4
pH
5.68
5.69
5.72
5.71
5.64


5
Particle
3.69 ± 2.45
3.54 ± 2.68
13.51 ± 2.78
3.68 ± 2.95
3.48 ± 2.66



size (μm)












Observations T = 14 days, 2-8° C.













1
Sedimentation 
0.96
0.96
0.96
0.95
0.90



Coefficient







2
Appearance 
two layers
two layers
two layers
two layers
opaque 



(visual)




supernatant








with 








floccules


3
Redispersibility
redispersible
redispersible
redispersible
redispersible
redispersible


4
pH
5.74
5.75
5.75
5.78
5.76


5
Particle
3.86 ± 2.86
3.57 ± 2.44
3.46 ± 2.58
3.76 ± 2.74
3.78 ± 2.68



size (μm)












Observations T = 14 days, 40° C./75% RH













1
Sedimentation 
0.74
0.78
0.39
0.33
0.60



Coefficient







2
Appearance 
clear 
clear 
clear 
clear 
clear 



(visual)
supernatant
supernatant
supernatant
supernatant
supernatant




with 
with 
with 
with 
with 




floccules
floccules
floccules, 
floccules, 
floccules,






an opaque 
an opaque 
an opaque 






flocculation
flocculation
flocculation






layer between 
layer between 
layer between 






clear
clear
clear






supernatant 
supernatant 
supernatant 






and
and
and






sedimentation
sedimentation
sedimentation


3
Redispersibility
redispersible
redispersible
redispersible;
redispersible;
redispersible






curdy, loose
curdy, loose







sedimentation 
sedimentation 







right
right







away after
away after







shaking
shaking



4
pH
5.32
5.32
5.40
5.40
5.32


5
Particle 
3.93 ± 3.00
3.86 ± 3.08
3.94 ± 3.03
3.96 ± 3.10
3.83 ± 2.72



size (μm)
















TABLE 10







Observations for freeze-thawing cycles of −10° C. (3-day) and RT (3-day)













DS-54
DS-55
DS-56
DS-57
DS-58


Formulation ID
Methocel F50
Methocel E50
Methocel A4MP
MC, 400 cps
Klucel JF


Polymer
3% w/v
3% w/v
0.5% w/v
0.5%. w/v
3% w/v







Observations after Freeze-Thawing Cycle: storage at −10° C. for 3 days followed by at RT for 3 days
















Sedimentation 
1 Cycle
0.96
0.96
0.48
0.34
0.66


Coefficient
2 Cycle
0.96
0.96
0.50
0.34
0.67



3 Cycle
0.96
0.96
0.50
0.34
0.54



4 Cycle
0.96
0.96
0.49
0.30
0.70



5 Cycle
0.96
0.96
0.49
0.38
0.79


Appearance (visual)
1 Cycle
uniform
uniform
clear supernatant
sedimentation 
opaque 






with floccules, 
two layers,
supernatant






a thin opaque
opaque 
with floccules






flocculation layer
supernatant







between clear
with floccules







supernatant and








sedimentation





2 Cycle
sedimentation 
uniform
clear 
sedimentation 
opaque 




two layers

supernatant
two layers, 
supernatant







opaque
with floccules







supernatant 








with floccules




3 Cycle
sedimentation 
uniform
clear supernatant,
opaque to clear
A thin clear




two layers

sedimentation 
supernatant,
supernatant 






two layers
sedimentation 
on top, a 







two layers
thick opaque








flocculation layer








between clear








supernatant and








sedimentation



4 Cycle
uniform
uniform
clear 
clear supernatant, 
opaque 






supernatant
a milky 
supernatant







flocculation
with floccules







layer 








between clear








supernatant and








sedimentation




5 Cycle
uniform
uniform
clear 
clear supernatant, 
opaque 






supernatant
a milky 
supernatant







flocculation
with floccules







layer 








between clear








supernatant and








sedimentation,








sedimentation 








two layers



Redispersibility
1 Cycle
redispersible
redispersible
redispersible;
redispersible
redispersible






sedimentation








occurs at 10 min








after shaking





2 Cycle
redispersible
redispersible
redispersible;
redispersible
redispersible






sedimentation








occurs at 10 min








after shaking





3 Cycle
redispersible
redispersible
redispersible;
redispersible;
redispersible






sedimentation
sedimentation







occurs at 10 min
occurs at 10 min







alter shaking, 
after shaking







sedimentation








appears loose 








and curdy





4 Cycle
redispersible
redispersible
redispersible:
redispersible;
redispersible






sedimentation
sedimentation







occurs at 10 min
occurs at 10 min







after shaking, 
after shaking







sedimentation








appears loose 








and curdy





5 Cycle
redispersible
redispersible
redispersible;
redispersible;
redispersible






sedimentation
sedimentation







occurs at 10 min
occurs at 10 min







after shaking, 
after shaking







sedimentation








appears loose 








and curdy




pH
1 Cycle
5.66
5.66
5.69
5.70
5.64



2 Cycle
5.62
5.63
5.66
5.69
5.57



3 Cycle
5.60
5.58
5.62
5.66
5.52



4 Cycle
5.61
5.56
5.60
5.61
5.54



5 Cycle
5.65
5.67
5.62
5.59
5.50


Particle 
1 Cycle
3.89 ± 3.33
3.88 ± 3.21
3.86 ± 2.81
3.92 ± 3.20
3.82 ± 2.46


size 
2 Cycle
3.94 ± 2.87
3.98 ± 2.97
3.97 ± 3.09
4.05 ± 3.25
3.99 ± 2.87


(μm)
3 Cycle
4.02 ± 3.25
4.08 ± 3.83
4.12 ± 3.15
4.06 ± 3.09
4.07 ± 3.69



4 Cycle
4.21 ± 3.07
4.27 ± 3.01
4.26 ± 3.29
4.25 ± 2.78
4.26 ± 2.86



5 Cycle
4.31 ± 3.04
4.29 ± 2.85
4.28 ± 2.99
4.32 ± 3.32
4.30 ± 3.30
















TABLE 11







Observations for Freeze-Thaw cycles of RT (3-day) and 2-8° C. (3-day)













DS-54
DS-55
DS-56
DS-57
DS-58


Formulation ID
Methocel F50
Methocel E50
Methocel A4MP
MC. 400 cps
Klucel JF


Polymer
3% w/v
3% w/v
0.5% w/v
0.5% w/v
3% w/v







Observations after Thawing-freeze Cycle: storage at RT for 3 days followed by at 2-8° C. for 3 days
















Sedimentation Coellicient
1 Cycle
0.97
0.97
0.53
0.96
0.62



2 Cycle
0.96
0.96
0.55
0.24
0.77



3 Cycle
0.96
0.96
0.53
0.23
0.70



4 Cycle
0.96
0.96
0.54
0.53
0.78



5 Cycle
0.94
0.90
0.52
0.51
0.56


Appearance (visual)
1 Cycle
sedimentation 
sedimentation 
clear supernatant, 
sedimentation 
opaque




two layers
two layers
a thin opaque
two layers
supernatant 






flocculation layer

with






between, clear

floccules






supernatant and








sedimentation,








sedimentation 








two layers





2 Cycle
sedimentation 
sedimentation 
clear supernatant
sedimentation 
opaque




two layers
two layers

two layers, 
supernatant 







milky
with







supernatant 
floccules







with








flocculus




3 Cycle
sedimentation 
sedimentation 
clear supernatant
An opaque
opaque




two layers
two layers

supernatant 
supernatant 







on top,
with







a thick milky
floccules







flocculation layer








between opaque








supernatant and








sedimentation,








sedimentation 








two layers




4 Cycle
sedimentation 
sedimentation two
clear supernatant
clear supernatant
opaque




two layers
layers

sedimentation 
supernatant 







two layers
with








floccules



5 Cycle
sedimentation 
clear to opaque
clear supernatant
clear supernatant
A thin clear




two layers
supernatant,

sedimentation 
supernatant on





sedimentation 

two
top, a thick





two layers

layers
opaque








flocculation 








layer








between clear








supernatant and








sedimentation


Redispersibility
1 Cycle
redispersible
redispersible
redispersible;
redispersible
redispersible






sedimentation 








occurs at 10 min 








after shaking





2 Cycle
redispersible
redispersible
redispersible:
redispersible
redispersible






sedimentation 








occurs at 10 min 








after shaking





3 Cycle
redispersible
redispersible
redispersible;
redispersible
redispersible






sedimentation 








occurs at 10 min 








after shaking





4 Cycle
redispersible
redispersible
redispersible;
redispersible;
redispersible






sedimentation 
sedimentation







occurs
occurs at 30 min







at 10 min 
after shaking







after shaking,








sedimentation 








appears








loose and curdy





5 Cycle
redispersible
redispersible
redispersible;
redispersible;
redispersible






sedimentation 
sedimentation







occurs at 10 min 
occurs at 10 min







after shaking,
after shaking







sedimentation 








appears








loose and curdy




pH
1 Cycle
5.70
5.77
5.71
5.70
5.63



2 Cycle
5.67
5.68
5.67
5.73
5.61



3 Cycle
5.61
5.58
5.70
5.65
5.53



4 Cycle
5.57
5.58
5.62
5.64
5.55



5 Cycle
5.62
5.58
5.57
5.62
5.47


Particle 
1 Cycle
4.10 ± 2.63
4.09 ± 3.04
3.96 ± 2.41
4.09 ± 3.14
4.08 ± 2.76


size (μm)
2 Cycle
4.31 ± 3.66
4.37 ± 3.29
4.13 ± 2.77
4.22 ± 2.45
4.16 ± 2.58



3 Cycle
4.36 ± 3.43
4.37 ± 3.01
4.36 ± 3.20
4.41 ± 3.42
4.41 ± 3.11



4 Cycle
4.44 ± 3.31
4.38 ± 3.19
4.47 ± 3.05
4.48 ± 3.23
4.46 ± 3.00



5 Cycle
4.47 ± 2.87
4.49 ± 2.98
4.47 ± 3.28
4.50 ± 3.06
4.48 ± 2.92









It was observed that suspensions with 3% Methocel F50 or 3% Methocel E50 as suspending agents showed good stability for 14 days and after 5 freeze-thawing cycles, but slow reconstitution rates were observed when dispersing blends in purified water.
Suspensions using 0.5% Methocel A4MP as suspending agent showed loose and curdy sedimentation during stability study, indicating incompatibility.
However, 0.5% Methylcellulose (400 cps) or 3% Klucel JF Pharm as suspending agents showed good reconstitution rate and acceptable stability and therefore could be used as alternative suspending agents for the target formulation.
Example 6: Optimization of Suspending Anent Concentration for Dispersibility Study (Using Simethicone Granular MED-342)
According to the observations of DS-54 to DS-58, concentrations of Methocel F50, Methocel E50, and Klucel JF Pharm were decreased for better dispersibility, while concentration of Methylcellulose 400 cps was increased for a balance between dispersibility and viscosity. Simethicone in the formulations was changed to the granular solid form (MED-342), which simplifies the process by avoiding adsorbing liquid simethicone on lactose. The compositions are shown in Table 12.







TABLE 12







Composition of blends using Simethicone granular solid











Quantity per unit (mg)




















S.#
Ingredients
DS-54
DS-55
DS-57
DS-58
DS-63
DS-64
DS-65
DS-66
DS-67
DS-68
DS-69
DS-70























1
Tecovirimat Granules
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50


2
Sucralose, NF
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00


3
Simethicone 
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00



granular solid,















USP (MED-342)














4
Lactose Monohydrate, 
483.50
483.50
608.50
483.50
508.50
508.50
583.50
508.50
533.50
533.50
558.50
533.50



NF (SuperTab 11SD)














5
Hypromellose 
150.00
—
—
—
125.00
—
—
—
100.00
—
—
—



(Methocel F 50)















Hypromellose 

150.00
—
—
—
125.00
—
—
—
100.00
—
—



(Methocel E 50)















Methyl Cellulose, 
—
—
25.00
—
—
—
50.00
—
—
—
75.00
—



400 cps, USP















Hydroxypropyl 
—
—
—
150.00
—
—
—
125.00
—
—
—
100.00



cellulose















(Klucel JF Pharm), 















NF














6
Purified water, 
—
—
—
—
—
—
—
—
—
—
—
—



USP, Q.S. to 5 mL































Total
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00
1000.00









In general, the formulation procedures include:

    
    
        1. blending of Tecovirimat granules and simethicone granular solid (MED-342), then passing through Quadro Comil equipped with 2B039R03125173*(991) screen;
        2. weighing of quantity equivalent to 40 g of Simethicone-Tecovirimat granules blend and mixing geometrically with other components;
        3. passing again of the blend through Quadro Comil using 2B039R03125173*(991) screen and
        4. dispersing of 1 dose in purified water to make Q.S. to 5 mL, followed by evaluation of dispersibility and dissolution (Table 13).
    
    









TABLE 13





Observation of reconstitution and dissolution



















Formulation ID












S.#
Properties
DS-54
DS-55
DS-57
DS-58





1
Chunks 
Big 
Big 
None
Very 



remaining
chunks
chunks

small 



at bottom 



chunks



after shaking





















Dissolution
Time
%
SD
%
SD
%
SD
%
SD


2
(n = 3)
(mins)
Released

Released

Released

Released







5
78.5
4.5
85.3
2.3
83.6
2.6
72.7
1.5




10
76.6
5.7
83.7
1.9
82.4
1.9
81.8
1.5




15
76.0
6.7
84.2
1.6
82.5
1.1
83.2
1.6




30
76.1
6.3
84.2
2.0
84.0
0.2
85.8
0.8




45
76.1
6.2
84.0
2.0
83.7
1.1
86.7
2.3














Formulation ID
















S.#
Properties
DS-63
DS-64
DS-65
DS-66
DS-67
DS-68
DS-69
DS-70





1
Chunks remaining at
Mediun
Medium
Small
None
Small
Small
Small
None



bottom after shaking
chunks
chunks
chunks

chunks
chunks
chunks









The % ST-246 is shown in FIG. 1.
It was observed that when concentrations of Methocel F50 and Methocel E50 decreased to 2.0% w/v (DS-67 and DS-68), there were still chunks remaining after shaking manually. Hydration rates of these two polymers are fast such that the gel layers developed inhibit the wetting of inside materials, leading to fish eyes and bad dispersibility. Concentration of 0.5-1.0% w/v Methylcellulose 400 cps and 2.0-2.5% w/v Klucel JF Pharm can reach a balance between good dispersibility and viscosity.
Example 7: Evaluation of a Combination of Suspending Agents
Combinations of suspending agents were used for dissolution study. Compositions arc given in Table 14. The Target formulation using Avicel CL-611 as a suspending agent exhibits floating in the form of lumps in dissolution test.







TABLE 14







Composition of solid blends using combination of suspending agents











Quantity per unit (ng)














Prototype 1
Prototype 2
Prototype 3
Prototype 4


S.#
Ingredients
(DS-59)
(DS-60)
(DS-61)
(DS-62)















1
Tecovirimat Granules
346.50
346.50
346.50
346.50


2
Simethicone granular 
10.00
10.00
10.00
10.00



solid, USP (MED-342)






3
Lactose Monohydrate, NF
328.50
348.50
373.50
370.50



(SuperTab 11SD)






4
Avicel CL-611, NF
50.00
25.00




5
Methyl Cellulose, 400 cps,
15.00
20.00
15.00
20.00



USP






6
Xantural 75
—
—
5.00
3.00


7
Purified water, USP,
—
—
—
—



Q.S. to 5 mL















Total
750.00
750.00
750.00
750.00









The formulation procedures was as follows;

    
    
        1. blending of Tecovirimat granules and Simethicone granular solid (MED-342), then passing through Quadro Comil equipped with 213039R03125173*(991) screen;
        2. weighing of the quantity equivalent to 30 units of Simethicone-Tecovirimat blend and then geometric mixing with other components; shaking and mixing of blend in a zip-lock bag;
        3. dispersing of 1 dose in purified water to make Q.S. to 5 mL for dissolution test (Table 15).
    
    









TABLE 15







Dissolution of suspensions using combination of suspending agents











Formulation ID














Prototype 1
Prototype 2
Prototype 3
Prototype 4


S.#
Properties
(DS-59)
(DS-60)
(DS-61)
(DS-62)















1
Dispersibility
Small chunks
Small chunks
Small chunks
Small chunks




remaining after
remaining after
remaining after
remaining after




shaking
shaking
shaking
shaking


2
Viscosity
Good viscosity
Watery
Watery
Watery


3
Dissolution
Instant dispersion
Instant dispersion
Gel clumps
Gel clumps




with formation of
with formation of






coarse particulates
line particulates









Example 8: Optimizing Anti-Foaming Agent and Viscosity
The suspensions were optimized for defoaming and viscosity of suspension by changing the level of simethicone solid granular and methylcellulose 400 cps in reference formulation DS-60. The methylcellulose 400 cps was replaced with Klucel JF Pharm and formulated to test the effect on foaminess. The compositions are given in Table 16.







TABLE 16







Composition of DS-60A to DS-60E and DS-71











Quantity per unit (mg)














S.#
Ingredients
DS-60A
DS-60B
DS-60C
DS-60D
DS-60E
DS-71

















1
Tecovirimat Granules
346.50
346.50
346.50
346.50
346.50
346.50


2
Simethicone granular
16.67
25.00
16.67
16.67
16.67
16.67



solid, USP (MED-342)








3
Lactose Monohydrate, 
341.83
333.50
336.83
331.83
331.83
341.83



NF (SuperTab 11SD)








4
Avicel CL-611, NF
25.00
25.00
25.00
25.00
25.00
25.00



Methyl Cellulose. 
20.00
20.00
25.00
30.00
37.50
—



400 cps, USP









Hydroxypropyl cellulose
—
—
—
—
—
20.00



(Klucel JF Pharm), NF








5
Purified water, 
—
—
—
—
—
—



USP, Q.S. to 5 mL



















Total
750.00
750.00
750.00
750.00
757.50
750.00









The formulation procedures included

    
    
        1. geometric mixing of quantity equivalent to 30 units of lactose monohydrate and simethicone (MED-342), then geometric mixing with other components;
        2. passing of the blend through Quadro Comil equipped with 2B039R03125173*(991) screen and
        3. dispersing of 1 dose in purified water to make Q.S. to 5 mL to test foaminess and viscosity (Table 17).
    
    









TABLE 17







Observation of foaminess and viscosity of DS-60A to DS-60E and DS-71











Formulation ID














S.#
Properties
DS-60A 
DS-60B
DS-60C
DS-60D
DS-60E
DS-71





1
Dispersibility
Easy to
Easy to
Easy to
Easy to
Small chunks
Easy to




disperse, 
disperse, 
disperse, 
disperse, 
remaining
disperse, 




no chunks
no chunks
no chunks
no chunks
after 
no chunks




remaining 
remaining
remaining
remaining
shaking
remaining


2
Foaminess
Less foam 
Decreased
Decreased
Decreased
Decreased
Less foam




than 
foam
foam
foam
foam
than 




DS-60




DS-60A


3
Viscosity
Watery
Watery
Watety
Watery
Good
Watery


















viscosity










It was observed that the level of 16.67-25 mg per unit of Simethicone (MED-342) attains a good anti-foaming effect. The replacement of Klucel F Pharm with Methylcellulose 400 cps also decreases foaminess. Good viscosity in suspension was observed when Methylcellulose 400 cps level reached 0.75% w/v.
Example 9: Methylcellulose (400 cps and 15 cps) Combination as Suspending Agents
The level of Methylcellulose 15 cps was adjusted for better dispersibility. Compositions are given in Table 18.







TABLE 18







Composition of DS-72 to DS-74









Quantity per unit (mg)











S.#
Ingredients
DS-72
DS-73
DS-74














1
Tecovirimat Granules
346.50
346.50
346.50


2
Simethicone granular solid, USP 
16.67
16.67
16.67



(MED-342)





3
Lactose Monohydrate, NF 
349.33
349.33
349.33



(SuperTab 11SD)





4
Methyl Cellulose, 400 cps, USP
37.50
33.50
30.00



Methyl Cellulose, 15 cps, USP
—
4.00
7.50


5
Purified water, USP,
—
—
—



Q.S. to 5 mL













Total
750.00
750.00
750.00









The formulation procedure included

    
    
        1. geometric mixing of Tecovirimat granules, lactose monohydrate and Simethicone (MED-342) and passing of blend through Quadro Comil equipped with 2B039R03125173*(991) screen;
        2. weighing of quantity equivalent to 10 units of blend and then geometric mixing with polymer(s) and
        3. dispersing of 1 dose in purified water to make Q.S. to 5 ml, to test dispersibility.
        It was observed that all three formulations were easy to disperse with no chunks remaining after shaking. With decreased level of Methylcellulose 400 cps in formulation, suspension was more watery, suggesting it's better to keep Methylcellulose 400 cps more than 0.75% w/v.
    
    


Example 10: Optimizing levels of Methylcellulose 15 cps and Simethicone in Methylcellulose Combination for Rapid Hydration
Methylcellulose 400 cps concentration was kept as 1.0% w/v, and the amount of Methylcellulose 15 cps was adjusted for better dispersibility/hydration and the level of Simethicone (MED-342) adjusted to investigate the anti-foaming effect. Compositions are given in Table 19. Total blends were decreased to 500 mg per unit.







TABLE 19







Composition of DS-75 to DS-82











Quantity per unit (mg)
















S.#
Ingredients
DS-75
DS-76
DS-77
DS-78
DS-79
DS-80
DS-81
DS-82



















1
Tecovirimat Granules
346.50
346.50
346.50
346.50
346.50
346.50
346.50
346.50


2
Sucralose, NF
10.00
10.00
10.00
10.00
10.00
10.00
10.00
10.00


3
Simethicone granular 
20.00
20.00
20.00
20.00
20.00
25.00
30.00
35.00



solid, USP (MED-342)










4
Lactose Monohydrate, 
43.50
63.50
53.50
43.50
58.50
53.50
53.50
53.50



NF (SuperTab 11SD)










5
Methyl Cellulose, 
50.00
50.00
50.00
50.00
50.00
50.00
50.00
50.00



400 cps, USP











Methyl Cellulose, 
—
5.00
15.00
25.00
10.00
15.00
15.00
15.00



15 cps, USP










6
Strawberry flavor,
5.00
5.00
5.00
5.00
5.00
5.00
5.00
5.00



#133.16296










7
Purified water, 
—
—
—
—
—
—
—
—



USP, Q.S. to 5 mL























Total
475.00
500.00
500.00
500.00
500.00
505.00
510.00
515.00









The formulation procedure was as follows;

    
    
        1. geometric mixing of quantity equivalent to 100 units of Tecovirimat granules, sucralose, lactose monohydrate, Simethicone (MED-342) and Methylcellulose 400 cps in DS-75, then passing of blend through Quadro Comil equipped with 2B039R03125173*(991) screen;
        2. for DS-75, weigh quantity equivalent to 50 units of blend and then geometric mixing with strawberry flavor;
        3. for DS-77, weigh quantity equivalent to 30 units of blend and then geometric mixing with strawberry flavor, Methylcellulose 15 cps, and lactose monohydrate;
        4. for DS-76 to DS-79, weigh quantity equivalent to 10 units of DS-75 and then geometric mixing with other components.
        5. for DS-80 to DS-82, weigh quantity equivalent to 5 units of DS-77 and then geometric mixing with other components and
        6. dispersing of 1 dose in purified water to make Q.S. to 5 mL to test dispersibility, foaminess and sedimentation (Table 20).
    
    









TABLE 20







Observation of suspensions of DS-75 to DS-82











Formulation ID
















S.#
Properties
DS-75
DS-76
DS-77
DS-78
DS-79
DS-80
DS-81
DS-82





1
Dispersibility
small
very
no
no
no
big
big
big



(chunks remaining at 
chunks
tiny
chunks
chunks
chunks
chunks
chunks
chunks



bottom after shaking)

chunk
















2 
Settlement/
T = 0
No sedimentation. Foam is similar in all formulations



foaminess
T = 30 min
No sedimentation. Individual bubbles are





smaller in DS-80, DS-81, DS-82




T = 1 hr
No sedimentation. Individual bubbles are 





smaller in DS-80, DS-81, DS-82





Quantity of foam is less inDS-80, DS-81, DS-82




T = 2 hr
No sedimentation. A very thin layer of foam 















in DS-80 and DS-81. No foam in DS-82.




T = 17 hr
Foaminess
No foam in all formulations





















Sedimentation
0.82
0.84
0.86
0.86
0.86
0.86
0.82
0.82





coefficient













Second 
4
4
4
4
4
4
5
5





layer (mm)









It was observed that methylcellulose 15 cps level of 10-25 mg per Tecovirimat dosage unit can lead to good dispersibility/hydration. There was no significant difference in foaminess of suspensions initially and after 17 hours of reconstitution in formations with Simethicone level of 20-35 mg per unit. The big chunks in formulation with 25-35 mg Simethicone per unit may be caused by Simethicone agglomeration, suggesting that it is advantageous to keep Simethicone as 20 mg per unit. All formulations showed good viscosity with 1% w/v Methylcellulose 400 cps.
Example 11: Scale-Up of the Optimized Compositions
Scale-up batches using methylcellulose (400 cps) and Klucel JXF Pharm as suspending agents were prepared and evaluated. Compositions are given in Table 21.







TABLE 21







Composition of Scale-Up batches











Quantity per unit (mg)












Goal: Using Methylcellulose (400





cps and 15 cps) as a suspending
Goal: Using Hydroxypropyl Cellulose




agent
(Klucel JXF Pharm) as a suspending




(500 g scale).
agent (500 g scale).














Batch: FSIG-
Batch: FSIG-
Batch: FSIG-
Batch: FSIG-




20140826-1
20140826-2
20141216-1
20141216-2


S. #
Ingredients
(DS-75)
(DS-77)
(DS-83)
(DS-84)















1
Tecovirimat
346.49
346.49
346.50
346.50



Granulate






2
Lactose
66.01
51.01
93.00
—



monohydrate, NF







(SuperTab 11SD)






3
Methylcellulose,
50.00
50.00
—
—



400 cps, USP






4
Methylcellulose,
—
15.00
—
—



15 cps, USP






5
Hydroxylpropyl
—
—
125.00
118.00



Cellulose (Klucel







JXF Pham)






6
Sucralose, NF
10.00
10.00
10.00
10.00


7
Strawberry flavor, 
5.00
5.00
—
—



#133.16296






8
Black cherry Nat
—
—
3.00
3.00



type flavor






9
Simethicone
20.00
20.00
20.00
20.00



Granular Solid







(MED-342), USP






10
Magnesium
2.50
2.50
2.50
2.50



stearate, NF















Total weight (mg)
500.00
500.00
600.00
500.00









The batch size of each composition was 0.500 kg. In general, the formulation procedure involved the following steps:

    
    
        1. Sieving of the Tecovirimat granules and granular Simethicone through Quadro Comil equipped with 2B039R03125173*(991) screen.
        2. Sieving of the Tecovirimat granulate and granular Simethicone through a #20 screen.
        3. Sieving of lactose through a #20 screen, then addition of Flavor on lactose with geometric mixing using spatula.
        4. Sieving of polymer and sucralose through a #40 screen.
        5. Blending of the Tecovirimat-Simethicone blend and other extra-granular components in the V-blender for 15 minutes.
        6. Passing of magnesium stearate through a #40 screen, then addition as a lubricant to above milled blend and lubricate for 5 minutes.
        7. Evaluation of the physicochemical properties of blend (Table 22).
        8. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 21).
        9. Packing of 20 doses of blend in Stick pack PAKVF2.5M Fin seal pouches (part #25M0275FS06) Size 2.5 inch×6 inch. Heat-seal properly. Prepare 15-20 pouches for stability/dissolution study (Table 24).
    
    









TABLE 22







Physicochemical properties of blends of scale-up batches














Batch: FSIG-
Batch: FSIG-
Batch: FSIG-
Batch: FSIG-


S#
Property
20140826-1
20140826-2
20141216-1
20141216-2





1
Appearance
White powder
White powder
White powder
White powder


2
Flow of Lubricated Blend
24
26
26
28



(Flodex Orifice mm)






3
Bulk Density (g/cc)
0.541
0.520
0.505
0.468



Tap Density (g/cc)
0.693
0.667
0.640
0.607



Compressibility Index (%)
21.9
22.0
21.1
22.9


4
Granules Retained on Sieve
0.01
0.01
0.02
0.02



# 20 (opening size 0.850







mm) (%)







Granules Retained on Sieve
1.85
2.22
3.29
3.52



# 30 (opening size 0.600







mm) (%)







Granules Retained on Sieve
9.37
10.46
8.60
10.61



# 40 (opening size 0.425







mm) (%)







Granules Retained on Sieve
8.64
9.72
10.72
12.48



# 50 (opening size 0.300







mm) (%)







Granules Retained on Sieve
4.12
4.36
4.06
5.24



# 60 (opening size 0.250







mm) (%)







Granules Retained on Sieve
10.53
11.84
12.33
12.29



# 80 (opening size 0.180







mm) (%)







Retained on Pan (size 
65.47
61.40
60.96
55.84



<0.180 mm) (%)







Mean Size (mm)
0.106
0.119
0.121
0.139
















TABLE 23





Physicochemical properties of suspensions of scale-up batches


















S#
Property
Batch: FSIG-20140826-1
Batch: FSIG-20140826-2





1
Reconstitution
After manual shaking,
After manual shaking, fish 




fish eyes were observed
eye was observed at 




at 4 positions at
only one position at 




bottom of bottle.
bottom of bottle.


2
Appearance 
White dispersion
White dispersion



after





reconstitution




3
pH of 
5.98
6.00



suspension




4
Microscopic
Particle size 3.16 ± 2.37 μm. 
Particle size 3.20 ± 2.63 μm. 



observation
No agglomeration observed.
No agglomeration observed.


5
Physical 
No phase separation or
No phase separation or



stability
agglomeration.
agglomeration.


6
Forced 
Redispersible
Redispersible



settling and





redispersibility
















7
Observation of
10 min
30 min
24 hr
10 min
30 min
24 hr



sedimentation













At 25° C.















Sedimentation
1.00
1.00
1.00
1.00
1 00
1.00



coefficient









Separation
None
None
Second
None
None
Second






layer


layer






(29%


(29%






height)


height)



Redispersibility
Redispersible
Redispersible
Redispersible 
Redispersible
Redispersible
Redispersible 









At 5° C.















Sedimentation
1.00
1.00
1.00
1.00
100
1.00



coefficient









Separation
None
None
Second
None
None
Second






layer


layer






(23%


(20%






height)


height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible













S#
Property
Batch: FSIG-20141216-1
Batch: FSIG-20141216-2





1
Reconstitution
After manual shaking,
After manual shaking, 




fish eyes were observed
no fish eye was 




at 2 positions at
observed, disperse 




bottom of bottle.
very well.


2
Appearance 
White dispersion
White dispersion



after





reconstitution




3
pH of 
5.99
6.15



suspension




4
Microscopic
Particle size 3.04 ± 2.11 μm. 
Particle size 3.13 ± 2.53 μm.



observation
No agglomeration observed.
No agglomeration observed.


5
Physical 
No phase separation. Small
No phase separation. Small



stability
agglomerations are observed
agglomerations are observed




when pouring out.
when pouring out.


6
Forced 
Redispersible
Redispersible



settling and





redispersibility
















7
Observation of
10 min
30 min
24 hr
10 min
30 min
24 hr



sedimentation













At 25° C.















Sedimentation
1.00
1.00
0.56
1.00
1.00
0.58



coefficient









Separation
None
None
None
None
None
None



Redispersibility
Redispersible 
Redispersible 
Redispersible 
Redispersible 
Redispersible
Redispersible







At 5° C.















Sedimentation
1.00
1.00
0.89
1.00
1.00
0.88



coefficient









Separation
None
None
None
None
None
None



Redispersibility
Redispersible 
Redispersible 
Redispersible
Redispersible 
Redispersble
Redispersible
















TABLE 24







Stability and dissolution of scale-up batches














Batch: FSIG-
Batch: FSIG-
Batch: FSIG-
Batch: FSIG-




20140820-1
20140826-2
201412145-1
20141216-2



Property
(DS75)
(DS77)
(DS83)
(DS84)



















Time
%

%

%

%



S#

(mins)
Released
SD
Released
SD
Released
SD
Released
SD




















1
Dissolution
5
88.6
1.3
88.4
1.5
84.2
6.7
92.4
4.2



(n = 6)
10
90.2
1.7
92.3
1.5
93.9
4.4
97.6
1.7




55
89.4
1.5
90.5
2.8
94.1
2.0
99.3
1.7




30
90.4
1.3
90.2
1.4
96.9
0.9
101.4
2.2




45
93.6
2.5
90.7
1.5
97.1
1.0
100.3
0.7









The % ST-246 released is shown in FIG. 2.
Example 12: Effect of Particle Sizes of API on Formulation Properties
To study the effect of particle sizes of API on formulation performance properties, such as dissolution, use API of different particle sizes for granulation process. Compositions are given in Table 25.







TABLE 25







Composition of DS-85 to DS-87 using API of different particle sizes











Quantity per unit (mg)




Goal: Using API of different particle sizes for granulation process (288.6 g scale). Ref. DS-




77 (Batch # FSIG-20140826-2)













Batch: FSIG-20150120-1
Batch: FSIG-20150120-2
Batch: FSIG-20150120-3


S. # 
Ingredients
(DS-85)
(DS-86)
(DS-87)










Intragranular Ingredients:











1 
Tecovirimat monohydrate,
209.0
209.0
209.0



micronized
using API Lot# SG-10C12-
using API Lot# SG-10C12-
using API Lot# SG-10C12-




T1039 (Trial #2, d90-21.51
T1039 (Trial #3, d90-38.74
T1039 (Trial #4, d90-81.53




μm)
μm)
μm)


2
Colloidal silicon dioxide, NF
1.95
1.95
1.95



(Cabosil M5P)





3
Croscarmellose sodium, NF
31.32
31.32
31.32



(AcDiSol)





4
Lactose monohydrate, NF
33.15
33.15
33.15



(SuperTab 11SD)





5
Microcrystalline cellulose. NF
49.62
49.62
49.62



(Avicel PH 101)













Intragranular Ingredients Total
325.04
325.04
325.04







Granulating Fluid:











6
Hypromellose, USP (Methocel E3
13.65
13.65
13.65



LV Prem.)





7
Sodium lauryl sulfate, NF
7.80
7.80
7.80


8 
Purified water, USP*
170.1
170.1
170.1



Granulating Fluid Solids Total
21.45
21.45
21.45










Granulate Solids
346.49
346.49
346.49







Extragranular Ingredients:











9
Lactose monohydrate, NF
51.01
51.01
51.01



(SuperTab 11SD)





10
Methylcellulose,
50.0
50.0
50.0



400 cps, USP





11
Methylcellulose,
15.0
15.0
15.0



15 cps, USP





12
Sucralose, NF
10.0
10.0
10.0


13
Strawberry flavor, #133.16296
5.0
5.0
5.0


14
Simethicone Granular Solid
20.0
20.0
20.0



(MED-342), USP





15
Magnesium stearate, NF
2.5
2.5
2.5










Powder for Reconstitution Blend Total
500.00
500.00
500.00





*Removed during the drying process.






The batch size of each composition was 0.2886 Kg. Detailed experimental procedures and results of each trial formulation were recorded in the executed batch records. In general, the formulation procedure involved the following steps:

    
    
        1. Passing of intragranular components through a #20 screen.
        2. Mixing of intragranular components containing diluents, disintegrant, in GMX-High Shear Granulator/Mixer (1 L bowl) at impeller blade speed 460 rpm and chopper speed of 2000 rpm for 2 minutes.
        3. Addition of granulating solution containing surfactant, binder (7.1% w/w Hypromellose Methocel E3 solution) and mixing with the intragranular components in GMX-High Shear Granulator/Mixer at impeller blade speed 460 rpm and chopper blade 2000 rpm for 8-10 minutes.
        4. Addition of about 1.5 g water to rinse the container, granulation for additional 2 minutes, recording of the visual observation of the wet mixture.
        5. Drying of wet granular mass using a Midi-Glatt fluid bed dryer/processor at inlet temperature 35-40° C. until the loss on drying (LOD) of the granules reached the range of 4.00-5.50% w/w, following are the typical drying parameters.
    
    
















Parameters
Units









Product Air Pressure (bar)
0.25-0.30



Inlet Air Temperature (° C.)
35



Filter Blowing Rate (Sec)
 1



Product Temperature (° C.)-at end
  20-21










    
    
        6. Passing of the dried granules through Quadro Comil equipped with 2R039R03125173*(991) screen.
        7. Weighing of the granules, then recalculation and weighing of extragranular ingredients.
        8. Passing of the Tecovirimat granules and granular Simethicone through Quadro Comil equipped with 2B039R03125173*(991) screen.
        9. Passing of the Tecovirimat granulate and granular Simethicone through a #20 screen.
        10. Passing of lactose through a #20 screen and addition of Flavor on lactose with geometric mixing using spatula.
        11. Passing of polymer and sucralose through a #40 screen.
        12. Blending of the Tecovirimat-Simethicone blend and other extragranular components in the V-blender for 15 minutes.
        13. Passing of magnesium stearate through a #40 screen, then addition as a lubricant to above milled blend and lubricate for 5 minutes.
        14. Evaluation of the physicochemical properties of blend (Table 26).
        15. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 27).
        16. Packing of 20 doses of blend in Stick pack PAKVF2.5M Fin seal pouches (part #25M0275FS06); size 2.5 inch×6 inch. Heat-seal properly. Prepare 3 pouches for dissolution study (Table 28).
    
    









TABLE 26







Characterization of blends DS-85 to DS-87













Batch: FSIG-
Batch: 
Batch: FSIG-




20150120-1
FSIG-20150120-2
20150120-3


S#
Property
(DS-85)
(DS-86)
(DS-87)





1
Appearance
White powder
White powder
White powder


2
Flow of Lubricated Blend 
24
24
24



(Flodex Orifice mm)





3
Bulk Density (g/cc)
0.454
0.436
0.426



Tap Density (g/cc)
0.605
0.590
0.568



Compressibility Index (%)
25.0
26.1
25.0


4
Granules Retained on Sieve # 20 
0.02
0.02
0.02



(opening size 0.850 mm) (%)






Granules Retained on Sieve # 30 
8.03
4.84
2.04



(opening size 0.600 mm) (%)






Granules Retained on Sieve # 40 
18.50
18.50
8.81



(opening size 0.425 mm) (%)






Granules Retained on Sieve # 50
14.86
12.11
10.99



(opening size 0.300 mm) (%)






Granules Retained on Sieve # 60 
5.32
3.60
6.14



(opening size 0.250 mm) (%)






Granules Retained on Sieve # 80 
37.04
28.28
38.65



(opening size 0.180 mm) (%)






Retained on Pan (size <0.180 
16.24
32.65
33.35



mm) (%)






Mean Size (mm)
0.252
0.204
0.168
















TABLE 27





Characterization of suspensions of DS-85 to DS-87




















Batch: FSIG-20150120-1
Batch: FSIG-20150120-2


S#
Property
(DS-85)
(DS-86)





1
Reconstitution
After manual shaking, no fish eyes is
After manual shaking, no fish eye is




observed, disperse very well.
observed, disperse very well.


2
Appearance after
White dispersion
White dispersion



reconstitution




3
pH of suspension
6.11
6.10


4
Microscopic
Particle size 4.10 ± 3.42 μm. No
Particle size 4.40 ± 3.52 μm. No



observation
agglomeration observed.
agglomeration observed.


5
Physical stability
No phase separation or agglomeration.
No phase separation or agglomeration.


6
Forced settling and
Redispersible
Redispersible



redispersibility














7
Observation of
10 min
30 min
24 hr
10 min



sedimentation













At 25° C.













Sedimentation
1.00
1.00
1.00
1.00



coefficient







Separation
None
None
Second layer
None






(22% height)




Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible









At 5° C.













Sedimentation
1.00
1.00
1.00
1.00



coefficient







Separation
None
None
Second layer
None






(22% height)




Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible















Batch: FSIG-20150120-2
Batch: FSIG-20150120-3



S#
(DS-86)
(DS-87)






1
After manual shaking, no fish eye is
After manual shaking, no fish eye is




observed, disperse very well.
observed, disperse very well.



2
White dispersion
White dispersion



3
6.10
6.12



4
Particle size 4.40 ± 3.52 μm. No
Particle size 4.84 ± 4.22 μm. No




agglomeration observed.
agglomeration observed.



5
No phase separation or agglomeration.
No phase separation or agglomeration.



6
Redispersible
Redispersible














7
30 min
24 hr
10 min
30 min
24 hr









At 25° C.













1.00
1.00
1.00
1.00
1.00



None
Second layer
None
None
Second layer




(22% height)


(26% height)



Redispersible
Redispersible
Redispersible
Redispersible
Redispersible









At 5° C.













1.00
1.00
1.00
1.00
1.00



None
Second layer
None
None
Second layer




(25% height)


(25% height)



Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
















TABLE 28







Dissolution profile of DS-85 to DS-87












Property
Batch: FSIG-20150120-1
Batch: FSIG-20150120-2
Batch: FSIG-20150120-3












Time
(DS-85)
(DS-86)
(DS-87)















S#

(mins)
% Released
SD
% Released
SD
% Released
SD


















1
Dissolution
5
72.5
1.6
58.8
1.5
36.0
1.2



(n = 6)
10
82.2
0.7
66.7
3.0
46.0
0.9




15
85.5
1.1
71.8
2.3
51.7
0.8




30
88.2
2.3
75.7
3.3
60.2
1.7




45
90.3
2.0
80.4
3.0
64.7
1.0









It was observed that DS-85 (API D90=21.51 μm) showed higher dissolution rate compared to DS-86 (API D90=38.74 μm) and DS-87 (API D90=81.53 μm). The particle size of the Tecovirimat monohydrate used for granulation is inversely proportional to the dissolution rate. Smaller particle API batches showed higher dissolution rate as; DS-85>DS-86>DS-87 (see FIG. 3).
Example 13: Optimization of Blending and Lubrication Time Tecovirimat Monohydrate Powder for Reconstitution
To study the effect of blend/lubrication time on blend uniformity and dissolution, sample where taken at different time points during a blending range of 10-25 minutes and a lubrication of 3-10 minutes. Composition is given in Table 29.







TABLE 29







Composition of DS-88











Quantity per unit (mg)




Goal: Blend or lube time DOE study 




(500 g scale).




Ref. DS-75 (Batch # FSIG-20140826-1)




Batch: FSIG-20150317-1


S. #
Ingredients
(DS-88)












1
Tecovirimat Granulate
346.49


2
Lactose monohydrate, NF 
66.01



(SuperTab 11SD)



3
Methylcellulose, 400 cps, 
50.00



USP



4
Sucralose, NF
10.00


5
Strawberry flavor,
5.00



#133.16296



6
Simethicone Granular Solid 
20.00



(MED-342), USP



7
Magnesium stearate, NF
2.50








Total weight (mg)
500.00









The batch size was 0.500 Kg. In general, the formulation procedure involved the following steps:

    
    
        1. Passing of the Tecovirimat granules and granular Simethicone through Quadro Comil equipped with 2B039R03125173*(991) screen;
        2. Passing of the Tecovirimat granulate and granular Simethicone through a #20 screen;
        3. Passing of lactose through a #20 screen, then addition of Flavor on lactose with geometric mixing using spatula;
        4. Passing of polymer and sucralose through a #40 screen;
        5. Blending of the Tecovirimat-Simethicone blend and other extragranular components in the V-blender, taking a 10 g sample at 10, 15, and 20 minutes. Halt the process at 25 minutes and repeat sampling;
        6. Passing of magnesium stearate through a #40 screen, then addition of 2.3 g as a lubricant to the remaining blend for lubrication. Take a 10 g sample at 3, 5, and 7.5 minutes. Final lubrication time is 10 minutes and repeat sampling;
        7. Evaluation of the physicochemical properties of blend (Table 30);
        8. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 31);
        9. Addition of Q.S. purified water to 10 g samples at different blending or lube time points to make 100 mL, then stirring well for tests of reconstitution and dissolution (Table 32A and 32 B).
    
    









TABLE 30







Characterization of Blend DS-88













Batch: FSIG-





20150317-1



S#
Property
(DS-88)







1
Appearance
White powder



2
Flow of Lubricated Blend (Flodex
24




Orifice mm)




3
Bulk Density (g/cc)
0.518




Tap Density (g/cc)
0.700




Compressibility Index (%)
26.0



4
Granules Retained on Sieve # 20 
0.01




(opening size 0.850 mm) (%)





Granules Retained on Sieve # 30 
4.09




(opening size 0.600 mm) (%)





Granules Retained on Sieve # 40 
10.36




(opening size 0.425 mm) (%)





Granules Retained on Sieve # 50 
9.20




(opening size 0.300 mm) (%)





Granules Retained on Sieve # 60 
4.03




(opening size 0.250 mm) (%)





Granules Retained on Sieve # 80 
14.76




(opening size 0.180 mm) (%)





Retained on Pan (size <0.180 mm) 
57.55




(%)





Mean Size (mm)
0.133

















TABLE 31







Characterization of suspension of DS-88











Batch: FSIG-20150317-1


S#
Property
(DS-88)





1
Reconstitution
After manual shaking, 1 fish eye is observed at bottom of bottle.


2
Appearance after
White dispersion



reconstitution



3
pH of suspension
6.13


4
Microscopic observation
Particle size 3.28 + 2.72 μm. No agglomeration observed.


5
Physical stability
No phase separation or agglomeration.


6
Forced settling and
Redispersible



redispersibility












7
Observation of sedimentation
10 min
30 min
24 hr









At 25° C.












Sedimentation coefficient
1.00
1.00
1.00



Separation
None
None
Second layer






(22% height)



Redispersibility
Redispersible
Redispersible
Redispersible









At 5° C.












Sedimentation coefficient
1.00
1.00
1.00



Separation
None
None
Second layer






(20% height)



Redispersibility
Redispersible
Redispersible
Redispersible
















TABLE 32A







Reconstitution and Dissolution of Samples at Different Blending Times (see FIG. 4)









Batch: FSIG-20150317-1 (DS-88)












Blending
Blending
Blending
Blending



10 minutes
15 minutes
20 minutes
25 minutes












S#
Property
(no lubricant)
(no lubricant)
(no lubricant)
(no lubricant)





1
Reconstitution
Tiny lumps, a little foam
Tiny lumps, a little foam
Tiny lumps, a little foam
Tiny lumps, a little foam






















Time
% 

% 

% 

% 





(mins)
Released
SD
Released
SD
Released
SD
Released
SD





2
Dissolution
5
90.6
1.0
92.5
2.4
90.2
1.1
92.3
1.2



(n = 6)
10
92.8
1.7
93.6
0.5
91.8
1.7
93.3
1.4




15
94.7
0.7
93.7
1.4
91.8
1.0
94.0
1.0




30
94.2
0.9
92.6
1.9
91.5
1.8
93.2
1.0




45
94.8
0.7
93.6
1.9
91.9
1.1
93.2
0.8
















TABLE 32B







Reconstitution and Dissolution of Samples at Different Lubrication Time (see FIG. 5)









Batch: FSIG-20150317-1 (DS-88)














Lubrication
Lubrication
Lubrication
Lubrication




3 minutes
5 minutes
7.5 minutes
10 minutes


S#
Property
(with lubricant)
(with lubricant)
(with lubricant)
(with lubricant)





1
Reconstitution
Tiny lumps, a little foam
Tiny lumps, a little foam
Tiny lumps, a little foam
Tiny lumps, a little foam





















% Released
SD
% Released
SD
% Released
SD
% Released
SD





2
Dissolution
93.6
1.6
92.9
1.1
90.0
1.1
95.5
1.3



(n = 6)
94.8
1.7
93.3
0.8
90.6
1.6
94.9
1.2




94.6
2.3
93.4
0.7
90.1
1.5
96.4
1.5




96.2
2.6
93.3
1.1
90.5
1.9
94.4
1.1




97.7
2.3
92.9
1.1
90.0
1.5
96.1
1.4









It was observed that all samples showed above 90% dissolution in 5 minutes, indicating that a blending range of 10-25 minutes and a lubrication range of 3-10 minutes have no significant effect on sample dissolution. Blending time and lubrication time is now fixed as 15 minutes and 5 minutes respectively as previous trials.
Example 31: Evaluation of Effect of High/Low Lubrication
To evaluate the effect of lubrication on +/−50% deviation of magnesium stearate level was used to test the effect of lubricant level on formulation physicochemical properties and dissolution. Compositions are given in Table 33.







TABLE 33







Composition of DS-89 and 90











Quantity per unit (mg)




Goal: High/low lubricant DOE study (500 g scale).




Ref. DS-75 (Batch # FSIG-20140826-1)












Batch: FSIG-20150325-1
Batch: FSIG-20150325-2




(DS-89)
(DS-90)




0.25% magnesium
0.75% magnesium


S. #
Ingredients
stearate
stearate













1
Tecovirimat Granulate
346.49
346.49


2
Lactose monohydrate, NF 
67.26
64.76



(SuperTab 11SD)




3
Methylcellulose, 400 cps, USP
50.00
50.00


4
Sucralose, NF
10.00
10.00


5
Strawberry flavor, #133.16296
5.00
5.00


6
Simethicone Granular Solid (MED-
20.00
20.00



342), ESP




7
Magnesium stearate, NF
1.25
3.75









Total weight (mg)
500.00
500.00









The batch sire of each composition was 0.500 kg. In general, the formulation procedure involved the following steps:

    
    
        1. Passing of the Tecovirimat granules and granular Simethicone through Quadro Comil equipped with 2B3039R03125173*(991) screen.
        2. Passing of the Tecovirimat granulate and granular Simethicone through a #20 screen.
        3. Passing of lactose through a #20 screen, then addition of Flavor on lactose with geometric mixing using spatula.
        4. Passing of polymer and sucralose through a #40 screen.
        5. Blending of the Tecovirimat-Simethicone blend and other extragranular components in the V-blender for 15 minutes.
        6. Passing of magnesium stearate through a #40 screen, then addition as a lubricant to above milled blend and lubricate for 5 minutes.
        7. Evaluation of the physicochemical properties of blend (Table 34).
        8. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 35).
        9. Packing of 20 doses of blend in Stick pack PAKVF2.5M Fin seal pouches (part #25M0275FS06); size 2.5 inch×6 inch. Heat-seal properly. Prepare 3 pouches for dissolution study (Table 36).
    
    









TABLE 34







Characterization of blend DS-89 and DS-90












Batch: FSIG-20150325-1
Batch: FSIG-


S#
Property
(DS-89)
20150325-2 (DS-90)





1
Appearance
White powder
White powder


2
Flow of Lubricated Blend (Flodex
24
24



Orifice mm)




3
Bulk Density (g/cc)
0.544
0.531



Tap Density (g/cc)
0.706
0.698



Compressibility Index (%)
22.9
23.9


4
Granules Retained on Sieve # 20
0.01
0.06



(opening size 0.850 mm) (%)





Granules Retained on Sieve # 30
4.32
4.30



(opening size 0.600 mm) (%)





Granules Retained on Sieve # 40
10.01
9.89



(opening size 0.425 mm) (%)





Granules Retained on Sieve # 50
10.89
13.49



(opening size 0.300 mm) (%)





Granules Retained on Sieve # 60
6.16
5.92



(opening size 0.250 mm) (%)





Granules Retained on Sieve # 80
15.86
16.26



(opening size 0.180 mm) (%)





Retained on Pan (size <0.180 mm)
52.74
50.08



(%)





Mean Size (mm)
0.145
0.153
















TABLE 35







Characterization of suspension of DS-89 and DS-90












Batch: FSIG-20150325-1
Batch: FSIG-20150325-2


S#
Property
(DS-89)
(DS-90)





1
Reconstitution
After manual shaking, no fish eye is
After manual shaking, 2 fish eyes are




observed, disperse very well.
observed at bottom of bottle.


2
Appearance after
White dispersion
White dispersion



reconstitution




3
pH of suspension
6.07
6.12


4
Microscopic
Particle size 3.22 ± 2.91 μm. No
Particle size 3.26 ± 3.00 μm. No



observation
agglomeration observed.
agglomeration observed.


5
Physical stability
No phase separation or agglomeration.
No phase separation or agglomeration.


6
Forced settling
Redispersible
Redispersible



and redispersibility
















7
Observation of
10 min
30 min
24 hr
10 min
30 min
24 hr



sedimentation















At 25° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(22% height)


(23% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible









At 5° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(20% height)


(36% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
















TABLE 36







Dissolution of DS-89 and DS-90 (see FIG. 6)











Property
Batch: FSIG-20150325-1
Batch: FSIG-20150325-2











Time
(DS-89)
(DS-90)












S#
(min)
% Released
SD
% Released
SD
















1
Dissolution
5
91.2
0.7
94.8
2.2



(n = 6)
10
92.6
0.5
95.5
1.8




15
92.9
1.1
95.1
1.5




30
92.8
1.1
95.6
2.3




45
93.8
1.8
95.8
2.0









The quantity of magnesium stearate at 0.25% and 0.75% level of the formulation did not show any difference in physicochemical properties as well as dissolution of the product.
Example 15: Effect of Milling on Particle Size and Size Distribution of Tecovirimat Granules
The purpose is to study the effect of milling on particle size and size distribution of Tecovirimat granules. 100 g of Tecovirimat granules from Siga were used to test the size distribution. 110 g of Tecovirimat granules from the same Siga Lot # were passed through Quadra Comil equipped with 2B039R03125173*(991) screen, from which 100 g were weighed for size distribution test. The results are shown in Table 37.







TABLE 37







Particle size and size distribution of Tecovirimat Granules 


before/after milling












Lot #
Lot #




1401165
1401165




(before
(after


S#
Property
milling)
milling)













1
Granules Retained on Sieve # 20 (opening 
0.05
0.04



size 0.850 mm) (%)





Granules Retained on Sieve # 30 (opening 
6.38
4.47



size 0.600 mm) (%)





Granules Retained on Sieve # 40 (opening 
10.34
12.31



size 0.425 mm) (%)





Granules Retained on Sieve # 50 (opening 
10.80
11.64



size 0.300 mm) (%)





Granules Retained on Sieve # 60 (opening 
5.05
5.36



size 0.250 mm) (%)





Granules Retained on Sieve # 80 (opening 
8.48
8.55



size 0.180 mm) (%)





Granules Retained on Sieve # 100 (opening 
4.74
4.70



size 0.150 mm) (%)





Retained on Pan (size <0.150 mm) (%)
54.17
52.93



Mean Size (mm)
0.150
0.150









It was observed that no significant different between the size distribution of Tecovirimat granules before or after milling.
Example 16: Evaluation of Pre-Dilution and as is Simethicone Before Mixing/Blending
Simethicone granular grade is currently prediluted/milled with Tecovirimat granules. The pre-dilution and no-dilution/as is Simethicone before mixing were compared, to establish the method of dilution that can be commercially reproduced at large scale. Compositions are given in Table 38.







TABLE 38







Composition of DS-91 and DS-92











Quantity per unit (mg)




Goal: Simethicone pre-dilution or no-dilution




(500 g scale). Ref. DS-75 (Batch # FSIG-




20140826-1)












Batch: FSIG-
Batch: FSIG-




20150407-1
20150407-2




(DS-91)
(DS-92)




pre-diluted
undiluted 


S. #
Ingredients
Simethicone
Simethicone













1
Tecovirimat Granulate
346.49
346.49


2
Lactose monohydrate, NF 
66.01
66.01



(SuperTab 11SD)




3
Methylcellulose, 400 cps, USP
50.00
50.00


4
Sucralose, NF
10.00
10.00


5
Strawberry flavor, #133.16296
5.00
5.00


6
Simethicone Granular Solid (MED-
20.00
20.00



342), USP




7
Magnesium stearate, NF
2.50
2.50









Total weight (mg)
500.00
500.00









The batch size of each composition was 0.500 kg. In general, the formulation procedure involved the following steps:

    
    
        1. For DS-91, passing of the Tecovirimat granules and granular Simethicone through Quadro Comil equipped with 2B039R03125173*(991) screen, then passing of the Tecovirimat granulate and granular Simethicone through a #20 screen;
        2. For DS-92, passing of the Tecovirimat granulate through Quadro Comil equipped with 2D039R03125173*(991) screen; then passing of granular Simethicone through a #20 screen;
        3. Passing of lactose through a #20 screen, then addition of Flavor on lactose with geometric mixing using spatula;
        4. Passing of polymer and sucralose through a #40 screen;
        5. Blending of Tecovirimat granulate and all the extragranular components in the V-blender for 15 minutes;
        6. Passing of magnesium stearate through a #40 screen, then addition as a lubricant to above milled blend and lubricate for 5 minutes;
        7. Evaluation of the physicochemical properties of blend (Table 39);
        8. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 40);
        9. Packing of 20 doses of blend in Stick pack PAKVF2.5M Fin seal pouches (part #25M0275FS06); size 2.5 inch×6 inch. Heat-seal properly. Prepare 3 pouches for dissolution study (Table 41).
    
    









TABLE 39







Characterization of blend DS-91 and DS-92












Batch: FSIG-
Batch: FSIG-




20150407-1
20150407-2


S#
Property
(DS-91)
(DS-92)





1
Appearance
White powder
White powder


2
Flow of Lubricated Blend (Flodex
24
26



Orifice mm)




3
Bulk Density (g/cc)
0.533
0.525



Tap Density (g/cc)
0.701
0.700



Compressibility Index (%)
24.0
25.0


4
Granules Retained on Sieve # 20
0.03
0.06



(opening size 0.850 mm) (%)





Granules Retained on Sieve # 30
4.26
4.06



(opening size 0.600 mm) (%)





Granules Retained on Sieve # 40
10.10
10.49



(opening size 0.425 mm) (%)





Granules Retained on Sieve # 50
10.87
10.11



(opening size 0.300 mm) (%)





Granules Retained on Sieve # 60
3.50
4.18



(opening size 0.250 mm) (%)





Granules Retained on Sieve # 80
12.92
13.80



(opening size 0.180 mm) (%)





Retained on Pan (size <0.180 mm) 
58.32
57.30



(%)





Mean Size (mm)
0.133
0.135
















TABLE 40







Physicochemical Properties of Suspension of DS-91 and DS-92












Batch: FSIG-20150407-1
Batch: FSIG-20150407-2


S#
Property
(DS-91)
(DS-92)





1
Reconstitution
After manual shaking, no fish eye is
After manual shaking, no fish eye is




observed, disperse very well.
observed, disperse very well.


2
Appearance after
White dispersion.
White dispersion.



reconstitution
Foaminess similar to DS-92
Foaminess similar to DS-91.


3
pH of suspension
6.11
6.05


4
Microscopic
Particle size 3.02 ± 2.03 μm. No
Particle size 3.14 ± 2.47 μm. No



observation
agglomeration observed.
agglomeration observed.


5
Physical stability
No phase separation or agglomeration.
No phase separation or agglomeration.


6
Forced settling
Redispersible
Redispersible



and redispersibility
















7
Observation of
10 min
30 min
24 hr
10 min
30 min
24 hr



sedimentation















At 25° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(28% height)


(33% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible









At 5° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(28% height)


(33% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
















TABLE 41







Dissolution of DS-91 and DS-92











Property
Batch: FSIG-20150407-1
Batch: FSIG-20150407-2











Time
(DS-91)
(DS-92)












S#
(min)
% Released
SD
% Released
SD
















1
Dissolution
5
94.7
2.3
92.2
1.2



(n = 6)
10
94.6
1.9
91.3
1.2




15
94.2
1.2
91.6
1.3




30
93.8
1.1
90.6
1.2




45
94.9
2.4
91.0
4.0









It was observed that before blending when passing through the #20 screen. Simethicone with no-dilution partly stuck on sieve (FIGS. 8 A and B), while the pre-diluted and co-milled Simethicone and Tecovirimat granules passed through #20 screen easily (FIGS. 8 C and D). After sieve shaking fir size distribution analysis, big clumps from DS-92 remained on #20 screen (FIG. 9), indicating the formation of big Simethicone lumps when no-dilution before mixing.
FIG. 8 shows an observation when passing simethicone or co-milled simethicone-Tecovirimat granules through #20 screen. A and B: no-dilution simethicone stuck on screen. C and D: pre-diluted and co-milled simethicone-Tecovirimat granules passed screen easily without sticking.
FIG. 9 shows retained clumps on #20 screen after sieve shaking of 100 g DS-92.
Example 17: Evaluation of Simethicone from Alternate Source
Currently Simethicone Granular Solid (MED-342) from Nusil Technologies was used. An alternate source of Simethicone-Simethicone 50% Powder from AIC was used to test the effect on formulation. The pre-dilution and no-dilution of simethicone before mixing were compared. Compositions arm given in Table 41.







TABLE 41







Composition of DS-93 and DS-94











Quantity per unit (mg)




Goal: Simethicone alternate source 




(500 g scale).




Ref. DS-75 (Batch # FSIG-20140826-1)












Batch: FSIG-
Batch: FSIG-




20150421-1
20150421-2




(DS-93)
(DS-94)




pre-diluted
Undiluted 


S. #
Ingredients
simethicone
simethicone













1
Tecovirimat Granulate
346.49
346.49


2
Lactose monohydrate, 
74.01
74.01



NF (SuperTab 11SD)




3
Methylcellulose, 400 
50.00
50.00



cps, USP




4
Sucralose, NF
10.00
10.00


5
Strawberry flavor, 
5.00
5.00



#133.16296




6
Simethicone 50% 
12.00
12.00



Powder




7
Magnesium stearate, 
2.50
2.50



NF











Total weight (mg)
500.00
500.00









The batch size of each composition was 0.500 Kg. Detailed experimental procedures and results of each trial formulation were recorded in the executed batch records. In general, the formulation procedure involved the following steps:

    
    
        1. For DS-93, passing of the Tecovirimat granules and Simethicone powder through Quadro Comil equipped with 2D039R03125173*(991) screen, then passing of the Tecovirimat granulate and Simethicone powder through a #20 screen;
        2. For DS-94, passing of the Tecovirimat granulate through Quadro Comil equipped with 2B039R03125173*(991) screen; then passing of Simethicone powder through a #20 screen;
        3. Passing of lactose through a #20 screen, then addition of Flavor on lactose with geometric mixing using spatula;
        4. Passing of polymer and sucralose through a #40 screen;
        5. Blending of Tecovirimat granulate and all the extragranular components in the V-blender for 15 minutes;
        6. Passing of magnesium stearate through a #40 screen, then addition as a lubricant to above milled blend and lubricate for 5 minutes;
        7. Evaluation of the physicochemical properties of blend (Table 43);
        8. Transfer of 10 doses of blend to a 100 cc glass bottle. Add water Q.S. to 50 mL. Shake well to mix the content. Evaluate the physicochemical properties of suspension (Table 44);
        9. Packing of 20 doses of blend in Stick pack PAKVF2.5M Finseal pouches (part #25M0275FS06); size 2.5 inch×6 inch. Heat-seal properly. Prepare 3 pouches for dissolution study (Table 45).
    
    









TABLE 43







Characterization of blend DS-93 and DS-94












Batch: FSIG-
Batch: FSIG-




20150421-1
20150421-2


S#
Property
(DS-93)
(DS-94)





1
Appearance
White powder
White powder


2
Flow of Lubricated Blend (Flodex
20
20



Orifice mm)




3
Bulk Density (g/cc)
0.547
0.540



Tap Density (g/cc)
0.720
0.720



Compressibility Index (%)
24.0
25.0


4
Granules Retained on Sieve # 20
0.02
0.02



(opening size 0.850 mm) (%)





Granules Retained on Sieve # 30
3.23
2.81



(opening size 0.600 mm) (%)





Granules Retained on Sieve # 40
8.73
8.52



(opening size 0.425 mm) (%)





Granules Retained on Sieve # 50
9.28
8.48



(opening size 0.300 mm) (%)





Granules Retained on Sieve # 60
3.56
3.75



(opening size 0.250 min) (%)





Granules Retained on Sieve # 80
13.96
11.28



(opening size 0.180 mm) ( %)





Retained on Pan (size <0.180 mm) 
61.23
65.15



(%)





Mean Size (mm)
0.118
0.108
















TABLE 44







Physicochemical properties of suspension of DS-93 and DS-94












Batch: FSIG-20150421-1
Batch: FSIG-20150421-2


S#
Property
(DS-93)
(DS-94)













1
Reconstitution
After manual shaking, no fish eye is
After manual shaking, no fish eye is




observed, disperse very well.
observed, disperse very well.


2
Appearance after
White dispersion, foaminess similar to
White dispersion, foaminess similar to



reconstitution
DS-94, more foam than DS-91 and DS-92.
DS-93, more foam than DS-91 and DS-92.


3
pH of suspension
6.11
6.07


4
Microscopic
Particle size 3.40 ± 3.04 μm. No
Particle size 3.46 ± 2.96 μm. No



observation
agglomeration observed.
agglomeration observed.


5
Physical stability
No phase separation or agglomeration.
No phase separation or agglomeration.


6
Forced settling and
Redispersible
Redispersible



redispersibility
















7
Observation of
10 min
30 min
24 hr
10 min
30 min
24 hr



sedimentation















At 25° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(26% height)


(29% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible









At 5° C.















Sedimentation
1.00
1.00
1.00
1.00
1.00
1.00



coefficient









Separation
None
None
Second layer
None
None
Second layer






(25% height)


(25% height)



Redispersibility
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
Redispersible
















TABLE 45







Dissolution of DS-93 and DS-94











Property
Batch: FSIG-20150421-1
Batch: FSIG-20150421-2











Time
(DS-93)
(DS-94)













S#

(min)
% Released
SD
% Released
% Released
















1
Dissolution
5
82.5
1.7
89.3
89.3



(n = 6)
10
84.7
1.4
86.8
86.8




15
85.4
0.7
86.9
86.9




30
86.5
1.1
88.2
88.2




45
86.2
1.9
88.4
88.4









It was observed that the undiluted simethicone 50% powder passed #20 screen easily without any sticking. The flowability of DS-93 and DS-94 using simethicone powder was better than that of previous batches using granular simethicone. The suspensions of DS-93 and DS-94 after reconstitution were similarly loamy, both containing much more foam than DS-91 and DS-92. Therefore the antifoaming effect of simethicone powder is not as efficient as that of granular simethicone.
Example 18: Taste Assessment of the Aqueous Pharmaceutical Suspension Formulations
The taste of the aqueous pharmaceutical suspension formulations according to the present invention is critically important to the successful oral administration.
Consequently the present invention provides a palatable powder for suspension dosage form of Tecovirimat which is suitable for pediatric and geriatric dosing
The following examples identify and quantify the sensory attributes (basic tastes, e.g., bitterness; aromatics and trigeminal effects) of a series of Tecovirimat aqueous suspensions.
Six experienced pharmaceutical sensory panelists were used to evaluate the above mentioned sensory attributes.
Samples were evaluated using the Flavor Profile method I of descriptive sensory analysis to identify, characterize and quantify the sensory attributes of the study samples. The Flavor Profile method was originally developed to assist The Upjohn Company in identifying off tastes in their gelatin capsules and provides a complete description of the sensory attributes of products. e.g., texture, aroma, taste, mouthfeel.
The method consists of formal procedures for describing and assessing the aroma (if appropriate) and flavor of a product in a reproducible manner. The Flavor Profile terms are shown in FIG. 11.
Flavor Profile is used to identify the individual attributes of product flavor, including:

    
    
        1. Basic tastes: sweet, sour, salty, and bitter;
        2. Aromatics: volatile components perceived by the olfactory system via the nasopharyngeal passage (retronasal) and
        3. Feeling factors: numbing, cooling, warming, burning, drying, oily, astringent, etc.
    
    


Flavor Profile also includes measures of the strength, or intensity, at which these character notes appear; the order in which the character notes appear; and a description of all sensations i.e. basic tastes, feeling factors, and aromatics remaining at specified time intervals after swallowing.
For some oral pharmaceuticals texture attributes such as hardness (chewable tablets) or particulates/grittiness (chewable tablets, sachets, suspensions) are also noted and assessed.
For oral pharmaceuticals, both the initial flavor quality and the aftertaste flavor quality are important to patient acceptability, and therefore it is important that each be evaluated. The initial flavor characteristics of the study products were evaluated during the first 10-20 seconds, and the aftertaste attributes were evaluated at multiple time intervals to 30 minutes as described below.
Sample Constitution and Taste Evaluation Protocols
The compounded Tecovirimat powder for suspension, micronized Tecovirimat API as well as tecovirimat granules taste assessment samples were constituted immediately prior to evaluations as follows:

    
    
        1. The API-containing powder in an amber bottle was agitated to ensure the powder moves around freely.
        2. The purified (reverse osmosis) water premeasured was added to each bottle containing the powder.
        3. The bottles were mixed by hand for a few minutes. The unflavored bottles were mixed vigorously and the flavored bottles were mixed gently.
        4. 5 mL samples were taken from each test formulation and dispensed to each panelist for evaluation.
    
    


The panelists evaluated the constituted samples as follows:

    
    
        1. The panelists cleansed their palates with spring water and unsalted crackers.
        2. 5 mL of sample was dispensed into individual 1-ounce plastic cups using a graduated oral syringe and distributed to each panelist.
        3. Starting at the same time, the panelists poured the sample directly in to their mouths, swished the contents around the oral cavity for 10 seconds and expectorated. During this time the panelists independently evaluated and recorded the initial flavor characteristics.
        4. The panelists then independently evaluated and recorded the aftertaste characteristics at periodic intervals out to 30 minutes as flavor persisted.
        5. The panelists recited their individual results and a preliminary Flavor Profile was generated for the sample.
        6. Steps 1 through 4 were repeated for a second evaluation of the sample using the preliminary Flavor Profile from Step 5 as a guide, with the panelists noting any necessary modifications.
        7. The panelists recited their individual results and a final Flavor Profile was developed for the sample.

Flavor Leadership Criteria

    
    


The flavor quality of the flavored drug product prototypes was interpreted using the relevant Flavor Leadership Criteria. Research reveals that the perennial sales leaders in many categories, including pharmaceuticals, have a set of sensory characteristics in common, which are known as the Flavor Leadership Criteria. The flavor profiles associated with products typically having the highest sales within a product category possess the following characteristics:

    
    
        1. Have a quickly recognizable identifying flavor;
        2. Develop full flavor that rapidly blends with and covers the active and base characteristics;
        3. Have no unpleasant mouth sensations (e.g., tongue sting);
        4. Have no off-notes in the early impression or in the aftertaste;
        5. Have a short (or appropriate) aftertaste.
    
    


The concepts of Flavor Leadership were used to interpret the sensory analysis results and are described more fully below.
Immediate Impact of the Identifying Flavor.
Flavor Leaders exhibit an immediate impact of the identifying flavor. When a consumer tastes a product, the first character notes provide for product identification and set expectations for the remainder of the flavor experience. For example, many inexpensive chocolates have an initial waxy impression whereas high-quality chocolates are identified by an initial chocolate flavor and cocoa butter mouthfeel. Unfortunately for many oral pharmaceuticals, the first impression is bitterness or an aromatic off-note (e.g., green stemmy, musty, chalky) that many patients find unacceptable.
Rapid Development of Balanced, Full Flavor.
A well-blended flavor is key to product success. Flavor Leaders have a flavor that develops rapidly and is full bodied and well balanced. Blended flavor means that a complex body of underlying sensory impressions that are not separately identified support the expected character notes. For example, Coca-Cola® comprises hundreds of individual flavoring components that are hard to single out individually, the components are very well blended. Unlike most foods and beverages, the challenge for pharmaceuticals is to “blend away” the negative sensory attributes of the drug substance, while simultaneously minimizing the number of excipients in the formulation. Amplitude is an integrative measure of balance and fullness (see FIG. 11). It is an overall measure of the quality of the initial flavor and has been shown to correlate with palatability and patient acceptance. Amplitude of 1 is appropriate for most oral pharmaceuticals.
Compatible Mouthfeel Factors.
Flavor leader have a mouthfeel that is compatible with consumers' expectations. Many drug actives and excipients can cause trigeminal effects such as tongue sting or throat burn that may be unacceptable to patients and consumers. For example, a slight amount of mouth irritation would be acceptable in a citrus flavored formulation (“citrus rind mouth irritation”) but would be totally out-of-context in a bubblegum flavored formulation. Unexpected or stronger than expected mouthfeel factors can have an adverse effect on patient acceptability.
Examples of excipients that produce trigeminal effects include:

    
    
        1. Methyl and propyl paraben (common preservatives in many oral pharmaceuticals) produce a tongue sting and numbing that can be unacceptable above a certain level.
        2. Benzyl alcohol (solvent/preservative) produces both tongue sting and bum.

No “Off-Flavors.”

    
    


Flavor leaders are notable for their consistent lack of off-flavors. An off-flavor is the appearance of an unexpected or unacceptable character note (off-note). For most oral pharmaceuticals the API is the principal source of off-notes, which can include basic tastes (e.g., bitter) and/or aromatics (e.g., sulfurous). Other sources of off-notes include excipients. e.g., paraben aromatics poor flavor systems that result in terpy, solventy, or perfumy off-notes, packaging interactions that “taint” or transfer of off-notes from the package to the product and “flavor scalping” or transfer (loss) of flavor aromatics from the product to the package.
Short (or Appropriate) Aftertaste.
The last impression i.e. the aftertaste is especially important to flavor quality. Aftertaste is caused by the persistence of one or a few character notes well after swallowing. For most products, a short, clean aftertaste is important. Products with a short, clean aftertaste encourage the consumer to take another bite or sip, and thereby to consume more of the product. For example, one of the major complaints about saccharin is its particularly long, bitter aftertaste and throat catch.
However, the goal for pharmaceuticals is patient compliance, not consumption. For many APIs, the aftertaste is most critical as many flavor systems provide adequate coverage in the early aftertaste but the beneficial effects quickly decrease, exposing the API.
As a general rule it is easier to mask a strongly bitter (or other) tasting API that “fades” quickly (steep decay curve) versus a moderately bitter API initially that lingers well into the aftertaste (flat decay curve). In any event, the challenge for the formulator is to mask the taste of the active throughout the duration of the aftertaste—be it 30 seconds or 30 minutes.
Drug Product
The study samples in Table 46 were compounded by Custom Medicine Pharmacenter, a compounding-only pharmacy in Beverly, Mass. following SIGA-approved batch records (logged formula worksheets). The Tecovirimat drug active was supplied by SIGA as bulk powder (granulated and micronized), and the excipients provided by SIGA approved suppliers.







TABLE 46







Study Formulations at 40 mg/mL Tecovirimat Concentration




















Form #3
Form #4






Form # 1
Form #2
(API
(API






(Strawberry
(Cherry
Granulated 200
Micronized 200






mg/ml)
mg/ml)
mg/ml)
mg/ml)






Batch Weight
Batch Weight
Batch Weight
Batch Weight


Ingredient
Manufacturer
Lot Number
Expiry
(g)
(g)
(g)
(g)





Tecovirimat, Granulate
SIGA
1401165
Sep. 14, 2015
3.465
3.465
3.465
—


Tracovirimat
SIGA
9199001
Aug. 25, 2016
—
—
—
2.090


Monohydrate, Micronized









Sucralose
EMD
K93441194
Mar. 8, 2016
0.100
0.100
—
—


Simethicone Granular
NUSIL
66777
May 12, 2015
0.200
0.200
—
—


Solid









Methocel, A4C
DOW
2H22012N11
Aug. 22, 2015
0.500
0.500

—


Strawberry Flavor,
Bell Flavors
521145
Jun. 23, 2016
0.500
—
—
—


133.1529









Cherry Flavor, 23950
Virginia Dare
T02198
Aug. 23, 2016
—
0.030
—
—


Lactose Monohyrdrate
DFE Pharma
10700181
Feb. 28, 2015
0.685
0.705
—
—


Purified Water
CMP
—
Dec. 31, 2015
47 mL
47 mL
47 mL
50 mL











Volume Tasted
50 mL
50 mL
50 mL
50 mL









Taste Assessment Days

The four formulations shown in Table 46 were evaluated over a three-day period as necessary to ensure that the maximum daily exposure of 600 mg per day was not exceeded. The schedule was as follows:
Day 1:
Tecovirimat Formulation 3 (200 mg/5 mL)—Tasted twice
Tecovirimat Formulation 4 (200 mg/5 mL)—Tasted once
Day 2:
Tecovirimat Formulation 4 (200 mg/5 mL)—Tasted once
Tecovirimat Formulation 1 (200 mg/5 mL)—Tasted twice
Day 3:
Tecovirimat Formulation 1 (200 mg/5 mL)—Tasted once
Tecovirimat Formulation 2 (200 mg/5 mL)—Tasted twice
Flavor Profile Results
The panelists evaluated the samples by the procedure outlined above. The results are summarized by formulation in tabular and graphical formats below. The tabular format contains the final Flavor Profile for the sample along with interpretation of the results using the Flavor Leadership Criteria.
The challenge for many oral pharmaceuticals is to mask the critical (“undesirable”) sensory characteristics of the active in the initial flavor and throughout the aftertaste.
To visualize the temporal sensory effects (aftertaste) it is useful to plot selected attribute intensities as a function of time. Thus, following each tabular summary is a graph. In each graph, the area above a slight intensity (>1) has been shaded. Negative sensory characteristics above this intensity are clearly perceptible to patients and are often found to be unacceptable.
To increase the likelihood of product acceptability the intensity of negative sensory characteristics should remain below this critical intensity throughout a product's flavor profile.
Tecovirimat—Formulation 3 (Granules)
Tecovirimat (200 mg/mL) Formulation #3 (Granule) is characterized by musty aromatic off notes, bitter basic taste and tannin/chalky mouth feels as shown in Table 47.







TABLE 47





Flavor Profile for Tecovirimat - Formulation 3 (Granules)







Flavor Profile SIGA Tecovirimat Formulation #3 200 mg/mL (Granule)

















Initial
1 Min
3 Min
5 Min
10 Min
15 Min
20 Min
25 Min
30 Min





Musty Aromatic
2
11⁄2
1
1⁄2
—
—
—
—
—


Sour
1⁄2
—
—
—
—
—
—
—
—


Bitter
1
1-11⁄2
1
1⁄2
1⁄2
1⁄2
0 —

—


Chalky Mouthfeel
1
1
1⁄2-1
1⁄2
—
—
—
—
—


Tannin Mouthfeel
11⁄2
1-11⁄2
1
1
—
—
—
—
—


Drying Mouthfeel
1⁄2
1
11⁄2
11⁄2
1-11⁄2
1-11⁄2
1
1⁄2
—










Flavor Leadership Interpretation











1 - Aromatic Identity
2- Amplitude
3- Mouthfeel
4- Off-Notes
5-Aftertaste





Not applicable
Not applicable
Chalky, tannin and
Slight-to-moderate
Lingering


for unflavored
for unflavored
drying mouthfeels
intensity bitterness
bitterness,


products
products

and moderate
mouthfeels and





intensity musty
aromatic off-notes





aromatic off-notes









The musty aromatic off-notes, bitterness and tannin mouthfeel of the Tecovirimat granules (Formulation #3) lingered at patient-perceptible levels (≥1) for about 3 minutes in the Aftertaste, see FIG. 12.
Tecovirimat—Formulation 4 (Micronized)
Tecovirimat (200 mg/mL) Formulation #4 (micronized API) is characterized by aromatic offnotes (chalky, soapy/waxy), bitter basic taste and tannin/chalky mouthfeels, though generally lower in intensity than the API granules as shown in Table 48.







TABLE 48





Flavor Profile for Tecovirimat - Formulation 4 (Micronized)







Flavor Profile SIGA Tecovirimat Formulation #4 200 mg/mL (Micronized API)

















Initial
1 Min
3 Min
5 Min
10 Min
15 Min
20 Min
25 Min
30 Min





Chalky Aromatic
1-11⁄2
1
1⁄2
—
—
—
—
—
—


Soapy/Waxy
1-11⁄2
1
1⁄2
—
—
—
—
—
—


Aromatic











Bitter
1
1-11⁄2
1⁄2-1
1⁄2
1⁄2
0-1⁄2
—

—


Chalky Mouthfeel
1⁄2-1
1
1-11⁄2
—
—
—
—
—
—


Tannin Mouthfeel
1
1
1
1⁄2-1
1⁄2
—
—
—
—


Drying Mouthfeel
1⁄2
1
1
1
1
1
1⁄2
0-1⁄2
—










Flavor Leadership Interpretation











1 - Aromatic Identity
2- Amplitude
3- Mouthfeel
4- Off-Notes
5-Aftertaste





Not applicable for
Not applicable
Chalky, tannin and
Slight-to-moderate
Lingering


unflavored products
for unflavored
drying mouthfeels
intensity bitterness
bitterness,



products

and moderate
mouthfeels and





intensity musty
aromatic off-notes





aromatic off-notes









The negative (aversive) sensory attributes of the micronized API (Formulation #4) are marginally above a slight (1) intensity, which is the perception threshold for most patients as shown in FIG. 13.
Tecovirimat—Formulation 1 (Strawberry)
Tecovirimat Formulation #1 (Strawberry) is somewhat low in initial flavor quality as measured by Amplitude (balance and fullness) as shown in Table 49.







TABLE 49





Flavor Profile for Tecovirimat - Formulation 1 (Strawberry)







Flavor Profile SIGA Tecovirimat Formulation #1 200 mg/mL (Strawberry)

















Initial
1 Min
3 Min
5 Min
10 Min
15 Min
20 Min
25 Min
30 Min





Amplitude
1










Sweet
11⁄2
1-11⁄2
1⁄2-1
1⁄2
0-1⁄2
—
—
—
—


Sour
1⁄2
—
—
—
—
—
—
—
—


Strawberry
11⁄2
1
1⁄2
—
—
—
—
—
—


Aromatics(Fresh











and Cream)











Chalky/Musty
11⁄2
1
1⁄2
0-1⁄2
—
—
—
—
—


Aromatic











Bitter
1
1⁄2-1
1⁄2
1⁄2
0-1⁄2
—
—
—
—


Chalky Mouthfeel
1
1
1
1⁄2
—
—
—
—
—


Tannin Mouthfeel
1⁄2-1
1
1⁄2-1
1⁄2
1⁄2
0-1⁄2
—
—
—


Drying Mouthfeel
—
1
11⁄2
1
1
1
1
1⁄2
—


Synthetic
—
1⁄2-1
1⁄2
0-1⁄2
—
—
1
—
—


Sweetener











Sensation (SSS)










Flavor Leadership Interpretation











1 - Aromatic Identity
2- Amplitude
3- Mouthfeel
4- Off-Notes
5-Aftertaste





Slight-to-moderate
A low level of
Chalky, tannin,
Slight intensity
Lingering basic


intensity strawberry
balance and
drying and SSS
bitterness and
tastes, aromatic off-


flavoring aromatics
fullness
mouthfeels
slight-to-moderate
notes and





intensity
mouthfeels





chalky/musty






aromatic off-notes









The flavor system (flavoring aromatics and sweet basic taste) of Formulation #1 (Strawberry) provides reasonable coverage of the bitter basic taste and chalky/musty aromatics as shown in FIG. 14.
Tecovirimat—Formulation 2 (Cherry)
As shown in Table 50, Tecovirimat Formulation #2 (Cherry) is slightly lower in initial flavor quality than the Formulation #1 (Strawberry) as measured by Amplitude.







TABLE 50





Tecovirimat - Formulation 2 (Cherry)







Flavor Profile SIGA Tecovirimat Formulation #2 200 mg/mL (Cherry)

















Initial
1 Min
3 Min
5 Min
10 Min
15 Min
20 Min
25 Min
30 Min





Amplitude
1⁄2-1










Sweet
11⁄2
1-11⁄2
1⁄2-1
1⁄2
0-1⁄2
0-1⁄2





Sour
1⁄2
0-1⁄2
—
—
—
—
—
—
—


Strawberry
11⁄2
1
1⁄2
—
—
—
—
—
—


Aromatics(Fresh











and Cream)











Chalky/Musty
11⁄2
1
1⁄2
—
—
—
—
—
—


Aromatic











Bitter
1
1
1⁄2-1
1⁄2
1⁄2
—
—
—
—


Chalky Mouthfeel
1
1-1⁄2
1
1⁄2
—
—
—
—
—


Tannin Mouthfeel
1
1-1⁄2
1
1⁄2
1⁄2
0-1⁄2
—
—
—


Drying Mouthfeel
—
1
1-11⁄2
1-11⁄2
1
1
1
1⁄2
—


Synthetic
—
1
1⁄2-1
1⁄2
1⁄2
—
—
—
—


Sweetener











Sensation (SSS)










Flavor Leadership Interpretation











1 - Aromatic Identity
2- Amplitude
3- Mouthfeel
4- Off-Notes
5-Aftertaste





Slight-to-moderate
A low level of
Chalky, tannin,
Slight intensity
Lingering basic


intensity strawberry
balance and
drying and SSS
bitterness and
tastes, aromatic off-


flavoring aromatics
fullness
mouthfeels
slight-to-moderate
notes and





intensity
mouthfeels





chalky/musty






aromatic off-notes









The flavor system (flavoring aromatics and sweet basic taste) of Formulation #2 (Cherry) provides reasonable coverage of the bitter basic taste and chalky/musty aromatics as shown in FIG. 14.
The results show that he unsweetened/unflavored Tecovirimat formulations are characterized by aromatic offnotes, (musty, chalky, soapy/waxy), bitter basic taste and tannin/chalky mouthfeels. However, overall, those attributes arc lower in intensity in the micronized API compared to the granules.
The negative attributes of the micronized API are near the perception threshold for most patients (i.e., may not be patient-perceptible).
The two sweetened/flavored Tecovirimat suspensions are somewhat low in overall flavor quality and both flavored suspensions are somewhat low in initial flavor quality as measured by Amplitude (Target=1.) due to their aromatic off-notes (chalky/musty), bitter basic taste and mouthfeels (tannin/chalky).
Both flavored suspensions arc somewhat low in flavor and sweetness impact (intensity). However the strawberry-flavored suspension is slightly more blended and full (i.e., higher in Amplitude) than cherry-flavored suspension, which was somewhat “solventy” (benzaldehyde-like) in character and both flavored suspensions provide a reasonable coverage of the bitter basic taste and chalky/musty aromatics, though the flavoring aromatics fade quickly.
Thus it can be seen that Tecovirimat is relatively “bland” in flavor (basic tastes, aromatics, mouthfeel and texture), with the micronized API having a lower flavor than the API granules and the sweetened/flavored formulations are somewhat low in flavor quality (strawberry higher than cherry), primarily due to low impact (intensity) and duration of the flavoring aromatics and to a lesser extent the underlying sweet basic taste.
While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made, and equivalents may be substituted, without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of tile invention.
REFERENCES


    1. Fenner et al., The epidemiology of smallpox. In: Smallpox and its eradication. Switzerland; World Health Organization; 1988)
    2. Bray et al., Antiviral Research 58: 101-114 (2003).
    3. Quenelle et al. 2007. Efficacy of delayed treatment with ST-246 given orally against systemic orthopoxvirus infections in mice. Antimicrobial Agents and Chemotherapy February; 51(2):689-95
    4. Smee et al. (2002) Antimicrob. Agents Chemother. 46:1329-1335)
    5. Vora et al., 2008, Severe eczema vaccinatum in a household contact of a smallpox vaccine. Clinical Infectious Disease 15; 46(10):1555-61).

## Claims

1. A dry suspension comprising 4-trifluoromethyl-N-(3,3a,4,4a,5,5a,6,6a-octahydro-1,3-dioxo-4,6-ethenocycloprop[f]isoindol-2-(1H)-yl)-benzamide (Tecovirimat (ST-246)) and simethicone.

2. A dry suspension according to claim 1 further comprising at least one suspending agent.

3. A dry suspension according to claim 2 wherein the suspending agent is methylcellulose.

4. A dry suspension according to claim 2 wherein the suspending agent is hydroxypropyl cellulose.

5. A dry suspension according to claim 1 further comprising a lubricant.

6. A dry suspension according to claim 5 wherein the lubricant is magnesium stearate.

7. A dry suspension according to claim 1 further comprising an excipient.

8. A dry suspension according to claim 7 wherein the excipient is lactose monohydrate.

9. A dry suspension according to claim 1 wherein the ST-246 is selected from a group consisting of ST-246 polymorph Form I, ST-246 polymorph Form II, ST-246 polymorph Form III, ST-246 polymorph Form IV, ST-246 polymorph Form V and ST-246 polymorph Form VI.

10. A dry suspension according to claim 1 wherein the ST-246 is micronized.

11. A dry suspension according to claim 1 wherein the ST-246 is granulated.

12. A dry suspension according to claim 1 wherein the simethicone is granular form.

13. A dry suspension according to claim 1 wherein the simethicone is in liquid form and adsorbed on lactose monohydrate.

14. A dry suspension according to claim 1 comprising 0.2 to 6.0 wt % simethicone.

15. A dry suspension according to claim 3 comprising 1 to 5 wt % methylcellulose.

16. A dry suspension according to claim 4 comprising 1.0 to 30 wt % hydroxypropylcellulose.

17. A dry suspension according to claim 1 wherein the particle size of ST-246 is 0.5 to 10 μm.

18. An aqueous pharmaceutical suspension formulation comprising a dry suspension according to claim 1 and water.

19. A formulation according to claim 18 further comprising a pharmaceutically acceptable ingredient.

20. A formulation according to claim 19 wherein the pharmaceutically acceptable ingredient is selected from the group consisting of carrier, excipient, diluent, additive, filler, lubricant and binder.

21. A method of treating orthopoxvirus infections and/or eczema vaccinatum comprising oral administration to a subject in need thereof a formulation according to claim 18.

22. The method of claim 21, wherein the subject is administered 400 mg to 2000 mg daily of ST-246.

23. A process of making the dry suspension according to claim 1, comprising mixing ST-246 with simethicone and optionally at least one suspending agent, at least one lubricant, at least one excipient, at least one further anti-foaming agent, at least one sweetener and/or at least one flavoring.