# Patent

## Title
Processes for isotopic modification of polyunsaturated fatty acids and derivatives thereof

## Abstract

Disclosed herein are methods for preparing isotopically modified polyunsaturated lipids containing 1,4-diene systems involving selective isotopic modification of one or more bis-allylic positions of the polyunsaturated lipids in the presence of a transition metal catalyst.

## Background
CROSS-REFERENCES TO RELATED APPLICATIONS
This application claims priority to U.S. Provisional Application No. 62/979,627 filed Feb. 21, 2020, which is incorporated herein in its entirety for all purposes.


BACKGROUND
Field
Isotopically modified polyunsaturated lipids, mixture of isotopically modified polyunsaturated lipids, methods of making such compounds or mixtures thereof, pharmaceutical compositions and medicaments comprising such compounds or mixtures, and uses of such compounds or mixtures to treat, prevent or alleviate various diseases, disorders, or conditions related to lipid peroxidation are provided.
Description of the Related Art
Oxidative damage is implicated in a wide variety of diseases including, but not limited to, mitochondrial diseases, neurodegenerative diseases, neurodegenerative muscle diseases, retinal diseases, energy processing disorders, kidney diseases, hepatic diseases, lipidemias, cardiac diseases, inflammation, and genetic disorders.
While the number of diseases associated with oxidative stress are numerous and diverse, it is well established that oxidative stress is caused by disturbances to the normal redox state within cells. An imbalance between routine production and detoxification of reactive oxygen species (“ROS”) such as peroxides and free radicals can result in oxidative damage to cellular structures and machinery. Under normal conditions, a potentially important source of ROSs in aerobic organisms is the leakage of activated oxygen from mitochondria during normal oxidative respiration. Additionally, it is known that macrophages and enzymatic reactions also contribute to the generation of ROSs within cells. Because cells and their internal organelles are lipid membrane-enveloped, ROSs can readily contact membrane constituents and cause lipid oxidation. Ultimately, such oxidative damage can be relayed to other biomolecules within the membrane and the cell, such as proteins and DNA, through direct and indirect contact with activated oxygen, oxidized membrane constituents, or other oxidized cellular components. Thus, one can readily envision how oxidative damage may propagate throughout a cell give the mobility of internal constituents and the interconnectedness of cellular pathways.
Lipid-forming fatty acids are well-known as one of the major components of living cells. As such, they participate in numerous metabolic pathways, and play an important role in a variety of pathologies. Polyunsaturated Fatty Acids (“PUFAs”) are an important sub-class of fatty acids. An essential nutrient is a food component that directly, or via conversion, serves an essential biological function and which is not produced endogenously or in large enough amounts to cover the requirements. For homeothermic animals, the two rigorously essential PUFAs are linoleic (cis,cis-9,12-Octadecadienoic acid; (9Z,12Z)-9,12-Octadecadienoic acid; “LA”; 18:2; n-6) and alpha-linolenic (cis,cis,cis-9,12,15-Octadecatrienoic acid; (9Z,12Z,15Z)-9,12,15-Octadecatrienoic acid; “ALA”; 18:3; n-3) acids, formerly known as vitamin F (Cunnane S C. Progress in Lipid Research 2003; 42:544-568). LA, by further enzymatic desaturation and elongation, is converted into higher n-6 PUFAs such as arachidonic (AA; 20:4; n-6) acid; whereas ALA gives rise to a higher n-3 series, including, but not limited to, eicosapentaenoic acid (EPA; 20:5; n-3) and docosahexaenoic (DHA; 22:6; n-3) acid. Because of the essential nature of certain PUFAs or PUFA precursors, there are many known instances of their deficiency and these are often linked to medical conditions. Furthermore, many PUFA supplements are available over-the-counter, with proven efficiency against certain ailments.
PUFAs endow mitochondrial membranes with appropriate fluidity necessary for optimal oxidative phosphorylation performance. PUFAs also play an important role in initiation and propagation of the oxidative stress. PUFAs react with ROS through a chain reaction that amplifies an original event (Sun M, Salomon R G, J. Am. Chem. Soc. 2004; 126:5699-5708). However, non-enzymatic formation of high levels of lipid hydroperoxides is known to result in several detrimental changes. Indeed, Coenzyme Q10 has been linked to increased PUFA toxicity via PUFA peroxidation and the toxicity of the resulting products (Do T Q et al, PNAS USA 1996; 93:7534-7539). Such oxidized products negatively affect the fluidity and permeability of their membranes; they lead to oxidation of membrane proteins; and they can be converted into a large number of highly reactive carbonyl compounds. The latter include reactive species such as acrolein, malonic dialdehyde, glyoxal, methylglyoxal, etc. (Negre-Salvayre A, et al. Brit. J. Pharmacol. 2008; 153:6-20).
Site-selective isotopic reinforcement of polyunsaturated fatty acids at their bis-allylic sites has been identified as a unique approach in preventing oxidative damage in these molecules, which had been linked to neuronal and retinal diseases, atherosclerosis, and aging. Typical methods for preparation of site-selectively deuterated PUFAs require rather long, laborious, and expensive syntheses and sometimes produce unfavored side products. Smarun et al., J. Org. Chem. 2017, 82, 13115-13120. There exits a need to develop efficient catalytic processes for site-specific deuteration of PUFAs and analogous poly-alkenes.
SUMMARY
Some embodiments of the present disclosure relate to a method of for isotopic modification of a polyunsaturated lipid, comprising:
reacting the polyunsaturated lipid with an isotope-containing agent in a presence of a transition metal catalyst to obtain an isotopically modified polyunsaturated lipid having an isotope at one or more bis-allylic positions, wherein the isotope-containing agent comprises at least one isotope selected from the group consisting of deuterium, tritium, and a combinations thereof; and wherein the transition metal catalyst has a structure of Formula (I) or (II):

[ML1(L2)m(L3)n]pQk  (I)

[ML1(L2)m1(L3)n1]-L-[ML1(L2)m2(L3)n2]qQk  (II)

wherein:
M is selected from the group consisting of rhodium, iridium, and ruthenium;
L1 is C3-C10 cycloalkenyl, C4-C10 cycloalkynyl, C6-C10 aryl, 5 to 10 membered heteroaryl, or 3 to 10 membered heterocyclyl, wherein L1 is optionally unsubstituted with one or more RA;
each L2 is independently selected from the group consisting of imine, carbene, carbonyl, alkene, alkyne, nitrile, isonitrile, acetonitrile, ether, thioether, phosphine, pyridine, optionally substituted C3-C10 cycloalkenyl, optionally substituted C4-C10 cycloalkynyl, optionally substituted C6-C10 aryl, optionally substituted 5 to 10 membered heteroaryl, or optionally substituted 3 to 10 membered heterocyclyl;
each L3 is independently C1-C6 alkyl, NR1R2 or C1-C6 alkoxy;
each R1 and R2 is independently H, optionally substituted C1-C6 alkyl, optionally substituted C3-C10 cycloalkyl, optionally substituted C3-C10 cycloalkenyl, optionally substituted C4-C10 cycloalkynyl, optionally substituted C6-C10 aryl, optionally substituted 5 to 10 membered heteroaryl, or optionally substituted 3 to 10 membered heterocyclyl;
each RA is independently hydroxyl, halogen, cyano, nitro, optionally substituted C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 alkoxy, C1-C6 haloalkyl, C1-C6 haloalkoxy, or optionally substituted amino;
L is a C1-C6 alkylene, C2-C6 alkenylene or C2-C6 alkynylene linker;
each of m, m1, m2, n, n1 and n2 is independently an integer of 1, 2 or 3;
each of p and q is independently an integer of 1, 2, 3 or 4;
Q is an anion; and
k is 0, 1 or 2.
In some embodiments of the method, M is ruthenium. In some embodiments, the polyunsaturated lipid is a fatty acid (PUFA), a fatty acid ester, a fatty acid thioester, a fatty acid amide, and a phospholipid containing a fatty acid moiety.
Some embodiments of the present disclosure relate to a composition comprising one or more isotopically modified polyunsaturated lipids having an isotope predominantly at one or more bis-allylic positions, wherein the isotopically modified polyunsaturated lipids are prepared by a method described herein.


DETAILED DESCRIPTION
Embodiments of the present disclosure relate to processes of preparing isotopically polyunsaturated lipid(s) using a transition metal catalyzed reaction. The polyunsaturated lipid may be a fatty acid (PUFA), a fatty acid ester, a fatty acid thioester, a fatty acid amide, or a phospholipid containing a fatty acid moiety. In some embodiments, the processes provide deuterated polyunsaturated lipid or deuterated polyunsaturated lipid mixtures. In some embodiments, the methods described herein result in site-specific deuteration of polyunsaturated lipid, wherein the deuteration occurs at both bis-allylic positions and mono-allylic positions. In some further embodiments, the method may result in site-specific deuteration occurring predominantly or exclusively at the bis-allylic positions.
Definition
The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art. The use of the term “including” as well as other forms, such as “include”, “includes,” and “included,” is not limiting. The use of the term “having” as well as other forms, such as “have”, “has,” and “had,” is not limiting. As used in this specification, whether in a transitional phrase or in the body of the claim, the terms “comprise(s)” and “comprising” are to be interpreted as having an open-ended meaning. That is, the above terms are to be interpreted synonymously with the phrases “having at least” or “including at least.” For example, when used in the context of a process, the term “comprising” means that the process includes at least the recited steps, but may include additional steps. When used in the context of a compound, composition, formulation, or device, the term “comprising” means that the compound, composition, formulation, or device includes at least the recited features or components, but may also include additional features or components.
The term “about” as used herein, refers to a quantity, value, number, percentage, amount, or weight that varies from the reference quantity, value, number, percentage, amount, or weight by a variance considered acceptable by one of ordinary skill in the art for that type of quantity, value, number, percentage, amount, or weight. In various embodiments, the term “about” refers to a variance of 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2% or 1% relative to the reference quantity, value, number, percentage, amount, or weight.
As used herein, the “bis-allylic” position refers to the methylene group of 1,4-diene systems of the polyunsaturated lipid described herein (e.g., the Y substitution positions of the polyunsaturated lipid of Formula (I)). As used herein, the “mono-allylic” position refers to the methylene group adjacent to only one double bond but is not the bis-allylic position (e.g., the X substitution positions of the polyunsaturated lipid of Formula (I)). It is further exemplified in the following structure:




The term “polyunsaturated lipid,” as used herein, refers to a lipid that contains two or more unsaturated bonds, such as double or triple bonds, in its hydrocarbon chain. The polyunsaturated lipid here can be a polyunsaturated fatty acid, polyunsaturated fatty acid ester, polyunsaturated fatty acid thioester, polyunsaturated fatty acid amide, polyunsaturated fatty acid phosphate, or a phospholipid containing the polyunsaturated fatty acid residue.
In some aspects, an isotopically modified PUFA molecule may contain one deuterium atom, such as when one of the two hydrogens in a methylene group is replaced by deuterium, and thus may be referred to as a “D1” PUFA. Similarly, an isotopically modified PUFA molecule may contain two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen or fourteen deuterium atoms, may be referred to as a “D2”, “D3”, “D4”, “D5”, “D6”, “D7”, “D8”, “D9”, “D10”, “D11”, “D12”, “D13” or “D14” PUFA, respectively.
As used herein, “Ca to Cb” in which “a” and “b” are integers refer to the number of carbon atoms in an alkyl, alkenyl or alkynyl group, or the number of carbon atoms in the ring of a cycloalkyl, aryl, heteroaryl or heterocyclyl group. That is, the alkyl, alkenyl, alkynyl, ring of the cycloalkyl, ring of the aryl, ring of the heteroaryl or ring of the heterocyclyl can contain from “a” to “b”, inclusive, carbon atoms. Thus, for example, a “C1 to C4 alkyl” group refers to all alkyl groups having from 1 to 4 carbons.
As used herein, “alkyl” refers to a straight or branched hydrocarbon chain that comprises a fully saturated (no double or triple bonds) hydrocarbon group of 1 to 20 carbon atoms, or 1 to 10 carbon atoms, or 1 to 6 carbon atoms.
As used herein, “alkenyl” refers to an alkyl group that contains in the straight or branched hydrocarbon chain one or more double bonds. The alkenyl group may have 2 to 20 carbon atoms or 8 to 18 carbons.
As used herein, cycloalkylnyl refers a hydrocarbon ring system having 6 to 20 carbon atoms, or 8 to 20 carbon atoms and having 1 to 3 alkynyl groups contained within the ring system.
As used herein, “alkynyl” refers to an alkynyl group of 2 to 20 carbon atoms, 2 to 10 carbon atoms, or 2 to 6 carbon atoms.
As used herein, “cycloalkyl” refers to a completely saturated (no double or triple bonds) mono- or multi-cyclic hydrocarbon ring system. When composed of two or more rings, the rings may be joined together in a fused fashion. Cycloalkyl groups can contain 3 to 10 atoms in the ring(s) or 3 to 8 atoms in the ring(s). A cycloalkyl group may be unsubstituted or substituted. Typical cycloalkyl groups include, but are in no way limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. A cycloalkyl group may be unsubstituted or substituted.
As used herein, “aryl” refers to a carbocyclic (all carbon) monocyclic or multicyclic aromatic ring system of 6 to 14 ring atoms (including, e.g., fused, bridged, or spiro ring systems where two carbocyclic rings share a chemical bond, e.g., one or more aryl rings with one or more aryl or non-aryl rings). The number of carbon atoms in an aryl group can vary. For example, the aryl group can be a C6-C14 aryl group, a C6-C10 aryl group, or a C6 aryl group. Examples of aryl groups include, but are not limited to, benzene, naphthalene, and azulene. An aryl group may be substituted or unsubstituted.
As used herein, “heteroaryl” refers to a monocyclic or multicyclic aromatic ring system (a ring system with fully delocalized pi-electron system) that contain(s) one or more heteroatoms (for example, 1, 2 or 3 heteroatoms), that is, an element other than carbon, including but not limited to, nitrogen, oxygen and sulfur. The number of atoms in the ring(s) of a heteroaryl group can vary. For example, the heteroaryl group can contain 5 to 10 atoms in the ring(s), 6 to 10 atoms. Examples of heteroaryl rings include, but are not limited to, furan, furazan, thiophene, benzothiophene, phthalazine, pyrrole, oxazole, benzoxazole, 1,2,3-oxadiazole, 1,2,4-oxadiazole, thiazole, 1,2,3-thiadiazole, 1,2,4-thiadiazole, benzothiazole, imidazole, benzimidazole, indole, indazole, pyrazole, benzopyrazole, isoxazole, benzoisoxazole, isothiazole, triazole, benzotriazole, thiadiazole, tetrazole, pyridine, pyridazine, pyrimidine, pyrazine, purine, pteridine, quinoline, isoquinoline, quinazoline, quinoxaline, cinnoline and triazineA heteroaryl group may be substituted or unsubstituted.
As used herein, “heterocyclyl” refers to three-, four-, five-, six-, seven-, eight-, nine- and ten-membered monocyclic, bicyclic and tricyclic ring system wherein carbon atoms together with from 1 to 5 heteroatoms constitute said ring system. A heterocycle may optionally contain one or more unsaturated bonds provided the system is not aromatic. The heteroatom(s) is an element other than carbon including, but not limited to, oxygen, sulfur and nitrogen. Heterocyclyl groups may be unsubstituted or substituted. Examples of such “heterocyclyl” groups include but are not limited to, aziridine, oxirane, thiirane, azetidine, oxetane, 1,3-dioxin, 1,3-dioxane, 1,4-dioxane, 1,2-dioxolane, 1,3-dioxolane, 1,4-dioxolane, 1,3-oxathiane, 1,4-oxathiin, 1,3-oxathiolane, 1,3-dithiole, 1,3-dithiolane, 1,4-oxathiane, tetrahydro-1,4-thiazine, 2H-1,2-oxazine, maleimide, succinimide, barbituric acid, thiobarbituric acid, dioxopiperazine, hydantoin, dihydrouracil, trioxane, hexahydro-1,3,5-triazine, imidazoline, imidazolidine, isoxazoline, isoxazolidine, oxazoline, oxazolidine, oxazolidinone, thiazoline, thiazolidine, morpholine, oxirane, piperidine N-oxide, piperidine, piperazine, pyrrolidine, azepane, pyrrolidone, pyrrolidione, 4-piperidone, pyrazoline, pyrazolidine, 2-oxopyrrolidine, tetrahydropyran, 4H-pyran, tetrahydrothiopyran, thiamorpholine, thiamorpholine sulfoxide, thiamorpholine sulfone and their benzo-fused analogs (e.g., benzimidazolidinone, tetrahydroquinoline and/or 3,4-methylenedioxyphenyl). Examples of spiro heterocyclyl groups include 2-azaspiro [3.3] heptane, 2-oxaspiro [3.3] heptane, 2-oxa-6-azaspiro [3.3] heptane, 2,6-diazaspiro [3.] heptane, 2-oxaspiro [3.4] octane and 2-azaspiro [3.4] octane.
As used herein, a substituted group is derived from the unsubstituted parent group in which there has been an exchange of one or more hydrogen atoms for another atom or group. Unless otherwise indicated, when a group is deemed to be “substituted,” it is meant that the group is substituted with one or more substituents independently selected from C1-C6 alkyl, C1-C6 alkenyl, C1-C6 alkynyl, C1-C6 heteroalkyl, C3-C7 carbocyclyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, v haloalkyl, and C1-C6 haloalkoxy), C3-C7-carbocyclyl-C1-C6-alkyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), 5-10 membered heterocyclyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), 5-10 membered heterocyclyl-C1-C6-alkyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), aryl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), aryl(C1-C6)alkyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), 5-10 membered heteroaryl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), 5-10 membered heteroaryl(C1-C6)alkyl (optionally substituted with halo, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy), halo, cyano, hydroxy, C1-C6 alkoxy, C1-C6 alkoxy (C1-C6)alkyl (i.e., ether), aryloxy, sulfhydryl (mercapto), halo(C1-C6)alkyl (e.g., —CF3), halo(C1-C6)alkoxy (e.g., —OCF3), C1-C6 alkylthio, arylthio, amino, amino(C1-C6)alkyl, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, acyl, cyanato, isocyanato, thiocyanato, isothiocyanato, sulfinyl, sulfonyl, and oxo (═O). Wherever a group is described as “substituted” that group can be substituted with the above substituents. In some embodiments, substituted group(s) is (are) substituted with one or more substituent(s) individually and independently selected from C1-C4 alkyl, C1-C4 alkoxy, C1-C4 haloalkyl, C1-C4 halalkoxy, amino, hydroxy, and halogen.
As used herein, the term “thioester” refers to a structure in which a carboxylic acid and a thiol group are linked by an ester linkage or where a carbonyl carbon forms a covalent bond with a sulfur atom —C(═O)SRA, wherein RA may include hydrogen, optionally substituted C1-30 alkyl (branched or straight), optionally substituted C2-30 alkenyl (branched or straight), optionally substituted C2-30 alknynyl (branched or straight), or optionally substituted ring structure such as C6-10 aryl, heteroaryl, carbocyclyl, cycloalkyl or heterocyclyl. “Polyunsaturated fatty acid thioester” refers to a structure P—C(═O)SRA, wherein P is a polyunsaturated fatty acid described herein.
As used herein, the term “amide” refers to compounds or moieties of the structure —C(O)NRARB and RA and RB can independently be hydrogen, optionally substituted C1-30 alkyl (branched or straight), optionally substituted C2-30 alkenyl (branched or straight), optionally substituted C2-30 alknynyl (branched or straight), or optionally substituted ring structure such as C6-10 aryl, heteroaryl, carbocyclyl, cycloalkyl or heterocyclyl. “Polyunsaturated fatty acid amide” refers to a structure a structure P—C(═O)NRARB, wherein P is a polyunsaturated fatty acid described herein.
As used herein, the term “salt” as used herein is a broad term, and is to be given its ordinary and customary meaning to a person of ordinary skill in the art (and is not to be limited to a special or customized meaning).
It is to be understood that certain radical naming conventions can include either a mono-radical or a di-radical, depending on the context. For example, where a substituent requires two points of attachment to the rest of the molecule, it is understood that the substituent is a di-radical. For example, a substituent identified as alkyl that requires two points of attachment includes di-radicals such as —CH2—, —CH2CH2—, —CH2CH(CH3)CH2—, and the like. Other radical naming conventions clearly indicate that the radical is a di-radical such as “alkylene” or “alkenylene.”
It is understood that, in any compound described herein having one or more chiral centers, if an absolute stereochemistry is not expressly indicated, then each center may independently be of R-configuration or S-configuration or a mixture thereof. Thus, the compounds provided herein may be enantiomerically pure, enantiomerically enriched, or may be stereoisomeric mixtures, and include all diastereomeric, and enantiomeric forms. In addition it is understood that, in any compound described herein having one or more double bond(s) generating geometrical isomers that can be defined as E or Z, each double bond may independently be E or Z a mixture thereof. Stereoisomers are obtained, if desired, by methods such as, stereoselective synthesis and/or the separation of stereoisomers by chiral chromatographic columns.
Likewise, it is understood that, in any compound described, all tautomeric forms are also intended to be included.
As used herein, “predominantly” refers to about 50% or greater. In one embodiment, predominantly refers to greater than about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100%.
Unless otherwise stated, when a position is designated specifically as “H” or “hydrogen”, the position is understood to have hydrogen at its natural abundance isotopic composition. Unless otherwise stated, when a position is designated specifically as “D” or “deuterium”, the position has deuterium at an abundance that is at least 3206 times of the natural abundance of deuterium, which is 0.0156% (i.e., at least 50% deuterium incorporation). More specifically, the position may have deuterium at an abundance that is at least 3500 times (54.6% deuterium incorporation), 4000 times (62.4% deuterium incorporation), 4500 times (70.2% deuterium incorporation), 5000 times (78% deuterium incorporation), 5500 times (85.8% deuterium incorporation), 6000 times (93.6% deuterium incorporation), 6090 times (95% deuterium incorporation), 6250 times (97.5% deuterium incorporation), 6346 times (99% deuterium incorporation), or 6378 times (99.5% deuterium incorporation) of the natural abundance of deuterium.
As used herein, the term “isotope-containing agent” refers to a compound containing deuterium or tritium atoms at an abundance that is significantly higher than the natural abundance of each of the atoms. For deuterium-containing agent, it contains at least 3206 times of the natural abundance of deuterium, (i.e., at least 50% deuterium incorporation). In some further embodiments, the isotope-containing agent has at least 4000, 4500, 5000, 5500, 6000, 6090, 6250, 6346 or 6378 times of the natural abundance of deuterium.
As used herein, the term “isotopic purity” of an isotope-containing agent refers to the percentage of molecules containing heave atoms (e.g., D or T) relative to the total number of molecules including molecules with no heavy atoms. For example, when the isotope-containing agent is heavy water (i.e., D2O) with 95% isotopic purity, it means in every 100 water molecules, there are 95 D2O molecule and 5 H2O molecule. In some instances, the isotopic purity of an isotope-containing agent may be at least 50%, 65%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99%.
Methods of Site-Specific Isotopic Modification
Conventional deuteration of molecules containing one alkene using the transition metal as a catalyst often have problems, including that predominantly vinylic positions (hydrogen atom connected to a doubly bonded carbon atom) are selectively deuterated. Many alkenes contain movement-restricted double bonds. Limited examples of linear (movement-unrestricted) alkenes yielded positional isomers, and cis-to-trans isomerisation always accompanied the deuteration process and lack of any reports on H/D exchange involving polyunsaturated alkenes.
Selective and efficient deuteration of various polyalkenes (including PUFAs) at the bis-allylic sites by a Ru-based complex (such as [Ru(Cp)(ACN)3]±PF6− (Cp=cyclopentadiene; CAN=acetonitrile) using a deuterium source D2O is described in U.S. Pub. No. WO 2017/091279. This Ru catalyst was capable of performing H/D exchange (deuteration) at the bis-allylic positions of linolenic acid (LNN), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) or ester thereof with at least 95% of deuteration efficiency. In addition, no more than about 30% of deuteration occurs at the mono-allylic positions of these polyunsaturated fatty acid or esters thereof. However, when linoleic acid (LIN) or an alkyl ester (e.g., ethyl ester) was subjected to the catalytic procedure using this Ru catalyst, only mono-allylic sites were deuterated to about 95% efficiency.
Without being bound by any theory, it is believed that reason for LIN being an exception with respect to deuteration selectivity lies in the proposed mechanism in which a PUFA binds to the ruthenium centre with two double bonds while D2O molecule occupies the remaining coordination site (structure A, Scheme 1). This structure is believed to activate both the heavy water molecule (making it more acidic) as well as both the mono- and bis-allylic sites of the bound PUFA. In the next step, the H/D exchange occurs, through the help of one or more additional D2O molecules (structure B, Scheme 1) but at the CH2 site closer to the bound D2O molecule. This CH2 site could be either mono- or bis-allylic for LNN, ARA, EPA and DHA, but it appears that bis-allylic sites are favoured over the mono-allylic sites with respect to the deuteration process for these PUFAs. Nevertheless, in the case of LIN there is only one bis-allylic site, which is spatially far away from the bound D2O molecule (structure A, Scheme 1). Hence, this site may not be involved in the overall deuteration process. Since only the mono-allylic sites of LIN are spatially close to the bound D2O, these mono-allylic sites are indeed deuterated.




Based on this proposed mechanism, it is believed that in order to deuterated LIN predominantly or exclusively at the bis-allylic position the following factors are important: (1) LIN needs to coordinate to the transition metal (e.g., ruthenium) in the similar fashion as depicted in structures A, B or C in Scheme 1; (2) the isotope-containing agent (e.g., D2O) binding site to ruthenium needs to be occupied/blocked by another molecule in order to avoid mono-allylic deuteration; and (3) D2O that is present in solution may need to be acidified. It should be also noted that the presence of the cylopentadienyl ligand (Cp), or presumably some other ring-type ligand (e.g. benzene), may be important for the overall deuteration. Therefore, in order to achieve an optimal outcome several different approaches are proposed as described in details below.
Some embodiment of the present disclosure relate to a method of for isotopic modification of a polyunsaturated lipid, comprising:
reacting the polyunsaturated lipid with an isotope-containing agent in a presence of a transition metal catalyst to obtain an isotopically modified polyunsaturated lipid having an isotope at one or more bis-allylic positions, wherein the isotope-containing agent comprises at least one isotope selected from the group consisting of deuterium, tritium, and a combinations thereof; and wherein the transition metal catalyst has a structure of Formula (I) or (II):

[ML1(L2)m(L3)n]pQk  (I)

[ML1(L2)m1(L3)n1]-L-[ML1(L2)m2(L3)n2]qQk  (II)

wherein:
M is selected from the group consisting of rhodium, iridium, and ruthenium;
L1 is C3-C10 cycloalkenyl, C4-C10 cycloalkynyl, C6-C10 aryl, 5 to 10 membered heteroaryl, or 3 to 10 membered heterocyclyl, wherein L1 is optionally unsubstituted with one or more RA;
each L2 is independently selected from the group consisting of imine, carbene, carbonyl, alkene, alkyne, nitrile, isonitrile, acetonitrile, ether, thioether, phosphine, pyridine, optionally substituted C3-C10 cycloalkenyl, optionally substituted C4-C10 cycloalkynyl, optionally substituted C6-C10 aryl, optionally substituted 5 to 10 membered heteroaryl, or optionally substituted 3 to 10 membered heterocyclyl;
each L3 is independently C1-C6 alkyl, NR1R2 or C1-C6 alkoxy;
each R1 and R2 is independently H, optionally substituted C1-C6 alkyl, optionally substituted C3-C10 cycloalkyl, optionally substituted C3-C10 cycloalkenyl, optionally substituted C4-C10 cycloalkynyl, optionally substituted C6-C10 aryl, optionally substituted 5 to 10 membered heteroaryl, or optionally substituted 3 to 10 membered heterocyclyl;
each RA is independently hydroxyl, halogen, cyano, nitro, optionally substituted C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 alkoxy, C1-C6 haloalkyl, C1-C6 haloalkoxy, or optionally substituted amino;
L is a C1-C6 alkylene, C2-C6 alkenylene or C2-C6 alkynylene linker;
each of m, m1, m2, n, n1 and n2 is independently an integer of 1, 2 or 3;
each of p and q is independently an integer of 1, 2, 3 or 4;
Q is an anion; and
k is 0, 1 or 2.
In some embodiments of the transition metal catalyst of Formula (I) or (II), the transition metal catalyst is a ruthenium (Ru) catalyst.
In some embodiments, the blocking of the isotope-containing agent (e.g., D2O) coordination site may be achieved by using a ligand that will be (a) sterically small so it does not interfere with polyunsaturated lipid (e.g., PUFA) binding and (b) strongly bound to the transition metal (e.g., ruthenium) such that it is not displaced by PUFA binding. To achieve these purposes, ligand such as alkyl, amino or alkoxy groups may be used. An embodiment of preparing such catalyst is illustrated in Scheme 2 below.




Catalyst D would block the deuteration at the mono-allylic positions once LIN is added (i.e. formation of D′) while at the same time keeping the bis-allylic position activated and ready for deuteration if acidity of the medium is adequate. However, compound D and D′ are neutral compounds. There is some possibility that such neutral form might affect their solubility and/or catalytic capabilities in the overall reaction medium.
If a neutral transition metal catalyst (e.g., Catalyst D) is not compatible with the reaction mixture and/or underperform catalytically, a cationic analog of the catalyst may be used. In some embodiments, a benzene ligand may be used to replace cyclopentadiene (Cp) as this ligand switch would keep the overall charge of the complex positive. An embodiment of the synthesis of such catalyst is illustrated in Scheme 3 below.




In some embodiments, changing the acidity of the catalyst may improve the reaction efficiency. In some such embodiment, a Lewis acid may be used to tether to a portion of the catalyst (e.g., tethered to a ligand of the catalyst). The tethering process could potentially lower the amount of the Lewis acid needed to acidify the solution as this tethered fragment would be positioned in a close proximity to the activated (i.e. Ru bound) LIN. Embodiments of such catalyst (Catalysts F and F′) are illustrated below.




Alternatively, a dinuclear catalytic system of Formula (II) may also be used in the method described herein. An embodiment of the deuteration of linoleic acid in the presence of heavy water using a dinuclear Ru catalyst is illustrated in Scheme 4. In this example, one of the Ru centers (the left one) would have two sites blocked with ligands (i.e., ERn) allowing only D2O to bind while the other one (right) would have only one site blocked allowing LIN to bind.




In some embodiments of the transition metal catalyst of Formula (I) or (II), L1 is C3-C10 cycloalkenyl or C6-C10 aryl, each optionally substituted with one or more RA. In one embodiment, L1 is an unsubstituted cyclopentadienyl (Cp). In another embodiment, L1 is a substituted cyclopentadienyl with one RA. In another embodiment, L1 is an unsubstituted benzene. In another embodiment, L1 is a substituted benzene with one RA. In some further embodiments, L1 is C3-C10 cycloalkenyl or C6-C10 aryl, each substituted with one or more RA. In some such embodiments, RA is C1-C6 alkyl substituted with a Lewis acid. In some further embodiments, RA is C1-C6 alkyl substituted with B(R3)2, and wherein each R3 is independently H, halogen, C1-C6 alkyl, C1-C6 haloalkyl, or optionally substituted C6-C10 aryl. In some further embodiments, RA is C1-C6 alkyl substituted with B(C6H5)2.
In some embodiments of the transition metal catalyst of Formula (I) or (II), each L2 is independently nitrile, isonitrile, acetonitrile, or a phosphine. In some embodiments, each L2 is acetonitrile (CH3CN). In some embodiments, at least one L2 is a phosphine of the structure P(R4)3, wherein each R4 is independently C1-6 alkyl, C3-8 cycloalkyl, 4-10 membered heteroaryl, C6-10 aryl, each optionally substituted. In some further embodiments, P(R4)3 is P(t-Bu)2(C6H5). In some embodiments, P(R4)3 is 4-(tert-butyl)-2-(diisopropylphosphaneyl)-1H-imidazole. In some embodiments, each L2 is independently acetonitrile or optionally substituted cyclopentadienyl.
In some embodiments of the transition metal catalyst of Formula (I) or (II), each L3 is independently is methyl, methoxy, or N(CH3)2.
In some embodiments of the transition metal catalyst of Formula (I), m is 1 or 2. In some embodiments, n is 2 or 1. In some further embodiments, m+n=3. In some embodiments of the transition metal catalyst of Formula (II), each of m1 and m2 is 1 or 2. In some embodiments, each of n1 and n2 is 2 or 1. In some further embodiments, m1+n1=3 and/or m2+n2=3.
In some embodiments of the transition metal catalyst of Formula (I) or (II), k is 0. In some other embodiments, k is 1. In some such embodiments, Q is an anion with a single negative charge, such as is PF6−, Cl−, F−, I−, Br−, NO3−, ClO4−, BF4−, B(C1-C4 alkyl)4−, Al(C1-C4 alkyl)4−, B(C6-C10 aryl)4−, Al(C6-C10 aryl)4−, or a carborane anion. In one embodiments, Q is PF6−. In such embodiments, p and/or q is 1. In some other embodiments, Q is an anion with a double negative charge, e.g., SO42−. In such embodiments, p or q is 2.
Additional ligands that may be use in the transition metal catalyst described herein include amine ligands. Amine ligands can be monodentate or multidentate and include monoamine, diamine, and triamine moieties. Monoamines can have the formula of N(Rb)2, and exemplary monoamines include but are not limited to dialkylmonoamines (such as di-ra-butylamine, or DBA) and trialkylmonoamines (such as N,N-dimethylbutylamine, or DMBA). Suitable dialkylmonoamines include dimethylamine, di-ra-propylamine, di-ra-butylamine, di-sec-butyl amine, di-tert-butylamine, dipentylamine, dihexylamine, dioctylamine, didecylamine, dibenzylamine, methylethylamine, methylbutylamine, dicyclohexylamine, N-phenylethanolamine, N-(p-methyl) phenylethanolamine, N-(2,6-dimethyl) phenylethanolamine, N-(p-chloro)phenylethanolamine, N-ethylaniline, N-butyl aniline, N-methyl-2-methylaniline, N-methyl-2,6-dimethylaniline, diphenylamine, and the like, and combinations thereof. Suitable trialkylmonoamines include trimethylamine, triethylamine, tripropylamine, tributylamine, butyldimethylamine, phenyldiethylamine, and the like, and combinations thereof. Diamines can have the formula (Rb)2N—Ra—N(Rb)2, and exemplary diamines can include alkylenediamines, such as N,N′-di-ieri-butylethylenediamine, or DBEDA. Triamine refers to an organic molecule having three amine moieties, including but not limited to diethylene triamine (DETA), guanidine HCl, tetramethyl guanidine, and the like. For both the monoamine and diamine formula, Ra is a substituted or unsubstituted divalent residue; and each Rb is independently hydrogen, C1-C8 alkyl, or C6-10 aryl. In some examples, of the above formula, two or three aliphatic carbon atoms form the closest link between the two diamine nitrogen atoms. Specific alkylenediamine ligands include those in which Ra is dimethylene (—CH2CH2—) or trimethylene (—CH2CH2CH2—). Rb can be independently hydrogen, methyl, propyl, isopropyl, butyl, or a C4-C8 alpha-tertiary alkyl group. In some embodiments, the diamine can be ethylenediamine. In some embodiments, the triamine can be diethylenetriamine.
The alkylenediamine ligands can be monodentate or multidentate and examples include N,N,N′,N′ tetramethylethylene diamine (TMED), N,N′-di-tert-butylethylenediamine (DBEDA), N,N,N′,N′-tetramethyl-1,3-diaminopropane (TMPD), N-methyl-1,3-diaminopropane, N,N′-dimethyl-1,3-diaminopropane, N,N,N′-dimethyl-1,3-diaminopropane, N-ethyl-1,3-diaminopropane, N-methyl-1,4-diaminobutane, N,N′-trimethyl-1,4-diaminobutane, N,N,N′-trimethyl-1,4-diaminobutane, N,N,N′,N′-tetramethyl-1,4-diaminobutane, N,N,N′,N′-tetramethyl-1,5-diaminopentane, and combinations thereof. In some embodiments, the amine ligand is selected from di-ra-butylamine (DB A), N,N-dimethylbutylamine (DMB A), N,N′-di-tert-butylethylenediamine (DBEDA), and combinations thereof.
Additional ligands that may be use in the transition metal catalyst described herein include alkene ligands. Amine The alkene ligands described herein be monodentate or multidentate and include a molecule that has at least one non-aromatic carbon-carbon double bond and can include but are not limited to monoalkene and dialkene. Examples of the alkene ligand can include ethylene, propylene, butene, hexene, decene, butadiene, and the like.
The isonitrile ligands described herein is also called isocyanide, referring to a molecule having at least one —NC moiety and can be monodentate or multidentate and include but are not limited to monoisonitrile and diisonitrile ligands. Examples of monoisonitrile and diisonitrile include but are not limited to C1-10 alkyl-NC and CN—R—NC and R is a C1-10 alkylene, t-butyl-NC, methyl-NC, PhP(O)(OCH2CH(t-Bu)NC)2, PhP(O)(OCH2CH(Bn)NC)2 PhP(O)(OCH2CH(i-Pr)NC)2, PhP(O)(OCHCH3CH(i-Pr)NC)2, PhP(O)(OCH2CH(CH3)NC)2. Additional isonitrile ligands can be found in Naik et al., Chem. Commun., 2010, 46, 4475-4477, which is incorporated herein by reference in its entirety.
The nitrile ligands described herein refer to a molecule having at least one —CN moiety and can be monodentate or multidentate and include but are not limited to monoisonitrile and diisonitrile ligands. Examples of monoisonitrile and diisonitrile include but are not limited to C1_10 alkyl-CN and CN—R—CN and R is a C1-10 alkylene, acetonitrile, 1,3,5-cyclohexanetricarbonitrile, propionitrile, butyronitrile, glutaronitrile, pivalonitrile, capronitrile, (CH2)3CN, (CH2)4CN, (CH2)5CN. Additional nitrile ligands can be found in Lee et al., Inorganic and Nuclear Chemistry letters, v10, 10 (October 1974) p. 895-898, which is incorporated herein by reference in its entirety.
The ether ligands described herein refer to a molecule having at least one R—O—R moiety wherein each R is independently an alkyl or aryl radical and can be monodentate or multidentate and include monoether, diether, and triether ligands. Examples of the monoether, diether, triether, and other suitable ether include but are not limited to dimethyl ether, diethyl ether, tetrahydrofuran, dioxane, dimethoxyethane, diethylene glycol dimethyl ether, polyethylene glycol, and anisole.
The thioether ligands described herein refer to a molecule having at least one R—S—R moiety a wherein each R is independently an alkyl or aryl radical and can be monodentate or multidentate and include monothioether, dithioether, and trithioether ligands. Examples of the monothioether, dithioether, and trithioether include but are not limited to dimethylsulfide and methyl phenyl sulfide.
The imine ligands described herein refer to a molecule having at least one carbon nitrogen double bond moiety and can be monodentate or multidentate and include monoimine, diimine, and triimine ligands. Examples of imine ligand include but are not limited to 1,2-ethanediimine, imidazolin-2-imine, 1,2-diketimine, dimethylglyoxime, o-phenylenediamine, 1,3-diketimines, and glyoxal-bis(mesitylimine).
The carbene ligands as described herein refers to compounds having at least one divalent carbon atom with only six electrons in its valence shell when not coordinated to a metal. This definition is not limited to metal-carbene complexes synthesized from carbenes, but is rather intended to address the orbital structure and electron distribution associated with the carbon atom that is bound to the metal. The definition recognizes that the “carbene” may not technically be divalent when bound to the metal, but it would be divalent if it were detached from the metal. Although many such compounds are synthesized by first synthesizing a carbene and then binding it to a metal, the definition is intended to encompass compounds synthesized by other methods that have a similar orbital structure and electron configuration. Lowry & Richardson, Mechanism and Theory in Organic Chemistry 256 (Harper & Row, 1976) defines “carbene” in a way that is consistent with the way the term is used herein. The carbene ligands described herein can be monocarbene, dicarbene, and tricarbene. Examples of carbene ligands include but are not limited to 1,10-dimethyl-3,30-methylenediimidazolin-2,20-diylidene, 1,10-dimethyl-3,30-ethylenediimidazolin-2,20-diylidene, 1,10-dimethyl-3,30-propylenediimidazolin-2,20-diylidene, 1,10-dimethyl-3,30-methylenedibenzimidazolin-2,20-diylidene, 1,10-dimethyl-3,30-ethylenedibenzimidazolin-2,20-diylidene, 1,10-dimethyl-3.30-propylenediimidazolin-2,20-diylidene,




and n is 1, 2, or 3, and





Additional carbene ligands can be found in Huynh et al, Journal of Organometallic Chemistry, v696, 21, (October 2011), p. 3369-33′75, and Malty et al., Chem. Commun., 2013, 49, 1011-101, which are incorporated herein by reference in their entireties.

The pyridine ligands as described herein refer to a molecule having at least one pyridine ring moiety and can include monopyridine, dipyridine, and tripyridine ligands. Examples of the pyridine ligand include but are not limited to 2,2′-bypiridine, and 2,6-Di(2-pyridyl) pyridine.
The phosphine ligands as described herein refer to a molecule having at least one P(R4)3, and each R4 is independently selected from the group consisting of hydrogen, optionally substituted C1-15 alkyl, optionally substituted C3-8 cycloalkyl, optionally substituted C6-15 aryl, and optionally substituted 4-10 membered heteroaryl. The phosphine ligand can include monophosphine, bisphosphine, and trisphosphine. Examples of suitable phosphine ligand can include but are not limited to PH3, trimethylphosphine, triphenylphosphine, methyldiphenylphosphine, trifluorophosphine, trimethylphosphite, triphenylphosphite, tricyclohexylphosphine, dimethylphosphinomethane (dmpm), dimethylphosphinoethane (dmpe), PROPHOS, PAMP, DIPAMP, DIOP, DuPHOS, P(tBu)2Ph, 1,2-B is (diphenylphosphino)ethane (dppe), 1,1′-B is (diphenylphosphino)ferrocene (dppf), 4-(tert-butyl)-2-(diisopropylphosphaneyl)-1H-imidazole, P(t-Bu)2(C6H5).
In some embodiments of the method described herein, the isotope-containing agent is D2O, DO(C1-C12 alkyl) (e.g., DOCH3 or DOCD3), T2O, or TO(C1-C12 alkyl) (e.g., TOCH3 or TOCT3), or combinations thereof.
Reaction Medium
In some embodiments of the method described herein, the reaction of the polyunsaturated lipid with the isotope-containing agent is in an acidic reaction medium, such as an acidic aqueous solution, an acidic solvent, or an acidic solvent mixture, or combinations thereof. In some embodiments, the reaction medium may comprise one or more solvent selected from the group consisting of acetone, methanol, ethanol, 1-propanol, isopropanol, 2-butanol, 1,4-dioxane, acetonitrile, dichoromethane (DCM), toluene, dimethylsulfoxide (DMSO), acetic acid, dimethylcarbonate, ethyl acetate, ether, ethylene glycol, or N-methyl-2-pyrrolidone (NMP), and combinations thereof. In some such embodiments, In further embodiments, the reaction medium comprises heavy water (D2O) and acetone. In some such embodiments, the acidic reaction medium comprises one or more pH modifiers selected from the group consisting of an organic acid, an inorganic acid, an Lewis acid, and combinations thereof. For example, acidification of heavy water could be done by introducing either a Lewis acid or certain amount of DC1 (deuterium chloride) in the overall reaction solution. The level of acidity will need to be balanced between the ability for the system to perform the deuteration and the stability of the catalyst in this acidified solution. The presence of C1 might interfere with the catalyst function as it might bind to the transition metal such as ruthenium. In such situation, use of a Lewis acid (e.g. B(C6F5)3) might be more appealing because it is highly unlikely that will interfere with any catalytic functions.
Polyunsaturated Lipids
In some embodiments, the polyunsaturated lipid comprises a fatty acid, a fatty acid ester, a fatty acid thioester, a fatty acid amide, a fatty acid phosphate, or a phospholipid derivative of the fatty acid, or combinations thereof. In some further embodiments, the phospholipid contains a polyunsaturated fatty acid residue after an esterification or amidation reaction between the carboxyl group of the fatty acid and the hydroxyl or amino group of the phospholipid. In some such embodiments, the polyunsaturated lipid may have two or more carbon-carbon double bonds (such as 2, 3, 4, 5, 6, 7, 8, 9, or 10 carbon-carbon double bonds). In some further embodiments, the polyunsaturated lipid is an omega-3 fatty acid, an omega-6 fatty acid, or an omega-9 fatty acid, or an ester, amide, thioester, phosphate or phospholipid derivative thereof. In some embodiments, the polyunsaturated lipid is linoleic acid, linolenic acid, gamma linolenic acid, dihomo gamma linolenic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, or an ester thereof. In some further embodiments, the polyunsaturated fatty acid ester is an alkyl ester, a triglyceride, a diglyceride, or a monoglyceride.
In some embodiments of the method described herein, the polyunsaturated lipid is deuterated at one or more bis-allylic positions. In some such embodiments, the polyunsaturated lipid is deuterated at all bis-allylic positions. In some further embodiments, the polyunsaturated lipids further deuterated at one or more mono-allylic positions. In some embodiments, the deuterated polyunsaturated lipid is deuterated linoleic acid, deuterated linolenic acid, deuterated arachidonic acid, deuterated eicosapentaenoic acid, deuterated docosahexaenoic acid, or a salt or an ester thereof. In some further embodiments, the ester is an alkyl ester, a triglyceride, a diglyceride, or a monoglyceride. In further embodiments, the ester is an ethyl ester.
In some embodiments, the polyunsaturated lipid has a structure of Formula (III):




R is H or C1-C10 alkyl;
R′ is —OR5, —SR5, —O(CH2)CH(OR6a)CH2(OR6b), —NR7R8,




each R5 is independently H, optionally substituted C1-C21 alkyl, optionally substituted C2-C21 alkenyl, optionally substituted C2-C21 alkynyl, optionally substituted C3-C10 cycloalkyl, optionally substituted C6-C10 aryl, optionally substituted 4 to 10 membered heteroaryl, optionally substituted 3 to 10 membered heterocyclyl, a mono-saccharide, a di-saccharide, or an oligosaccharide;
each of R6a and R6b is independently H, optionally substituted —C(═O)C1-C21 alkyl, optionally substituted —C(═O)C2-C21 alkenyl, or optionally substituted —C(═O) C2-C21 alkynyl;
each of R7 and R8 is independently H, optionally substituted C1-C21 alkyl, optionally substituted C2-C21 alkenyl, optionally substituted C2-C21 alkynyl, optionally substituted C3-C10 cycloalkyl, optionally substituted C6-C10 aryl, optionally substituted 4 to 10 membered heteroaryl, or optionally substituted 3 to 10 membered heterocyclyl; or R7 and R8 together with the nitrogen atom to which they are attached form an optionally substituted 3 to 10 membered heterocyclyl;
each R9 is independently optionally substituted C1-C21 alkyl, optionally substituted C2-C21 alkenyl, optionally substituted C2-C21 alkynyl;
each R10 is independently H,




—CH2CH2NH2, —CH2CH2NH3+, CH2CH(NH2)C(═O)O−, —CH2CH(OH)CH2OH, a mono-saccharide, a di-saccharide, or an oligosaccharide;

R11 is optionally substituted C8-C21 alkyl, optionally substituted C8-C21 alkenyl, or optionally substituted C8-C21 alkynyl;
R12 is H,




a mono-saccharide, a di-sacchardie, or an oligosaccharide; and

each of p and q is independently an integer of 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10.
In some embodiments of the polyunsaturated lipid of Formula (III), R is methyl, C4 alkyl, or C7 alkyl, each optionally substituted. In other embodiments, R is unsubstituted.
In some embodiments, the method yields deuterated linoleic acid or a derivative thereof of Formula (IIIa) (where R is n-butyl, p=1, and q=6):




In some such embodiments, one or both Y is D. In some further embodiments, each X is H. In other embodiments, at least one of X is D. In some such embodiments, R′ is —OR5, wherein R5 is H or optionally substituted C1-C21 alkyl. In one embodiment, R5 is ethyl. In one such embodiment, the deuterated polyunsaturated lipid is 11,11-D2-linoleic acid (D2-Lin), a pharmaceutically acceptable salt thereof, or ethyl ester thereof.

In some embodiments, the method yields deuterated linoleic acid or a derivative thereof of Formula (IIIb) (where R is methyl, p=2, and q=6):




In some such embodiments, at least one Y is D. In some further embodiments, each Y is D. In some further embodiments, each X is H. In other embodiments, at least one of X is D. In some such embodiments, R′ is —OR5, wherein R5 is H or optionally substituted C1-C21 alkyl. In one embodiment, R5 is ethyl. In one such embodiment, the deuterated polyunsaturated lipid is 11,11,14,14-D4-linolenic acid, a pharmaceutically acceptable salt thereof, or ethyl ester thereof.

In some embodiments, the method yields deuterated arachidonic acid or a derivative thereof of Formula (IIIc) (where R is n-butyl, p=3, and q=2):




In some such embodiments, at least one Y is D. In some further embodiments, each Y is D. In some further embodiments, each X is H. In other embodiments, at least one of X is D. In some such embodiments, R′ is —OR5, wherein R5 is H or optionally substituted C1-C21 alkyl. In one embodiment, R5 is ethyl. In one such embodiment, the deuterated polyunsaturated lipid is 7,7,10,10,13,13-D6-arachadonic acid, a pharmaceutically acceptable salt thereof, or ethyl ester thereof.

In some embodiments, the method yields deuterated eicosapentaenoic acid or a derivative thereof of Formula (IIId) (where R is methyl, p=4, and q=2):




In some such embodiments, at least one Y is D. In some further embodiments, each Y is D. In some further embodiments, each X is H. In other embodiments, at least one of X is D. In some such embodiments, R′ is —OR5, wherein R5 is H or optionally substituted C1-C21 alkyl. In one embodiment, R5 is ethyl. In one such embodiment, the deuterated polyunsaturated lipid is 7,7,10,10,13,13,16,16-D8-eicosapentaenoic acid, a pharmaceutically acceptable salt thereof, or ethyl ester thereof.

In some embodiments, the method yields deuterated docosahexaenoic acid or a derivative thereof of Formula (IIIe) (where R is methyl, p=5, and q=1):




In some such embodiments, at least one Y is D. In some further embodiments, each Y is D. In some further embodiments, each X is H. In other embodiments, at least one of X is D. In some such embodiments, R′ is —OR5, wherein R5 is H or optionally substituted C1-C21 alkyl. In one embodiment, R5 is ethyl. In one such embodiment, the deuterated polyunsaturated lipid is 6,6,9,9,12,12,15,15,18,18-D10-docosahexaenoic acid, a pharmaceutically acceptable salt thereof, or ethyl ester thereof.

In other embodiments of the polyunsaturated lipid of Formula (III), the polyunsaturated lipid is in the form of a glyceride ester, wherein R′═—O(CH2)CH(OR6a)CH2(OR6b). When each of R6a and R6b is H, such ester is a mono-glyceride, when only one of R6a and R6b is H, such ester is a di-glyceride. When neither R6a nor R6b is H, such ester is a tri-glyceride.
Mixture of Deuterated Polyunsaturated Lipids
In some embodiments, the catalytic method described herein yields a mixture of polyunsaturated lipids described herein. In some such embodiments, at least one polyunsaturated lipid in the mixture is deuterated at all bis-allylic positions. In some further embodiments, one or more polyunsaturated lipids in the mixture is further deuterated at one or more mono-allylic positions. In other embodiments, none of polyunsaturated lipids in the mixture is deuterated at one or more mono-allylic positions. In some such embodiments, the mixture of the polyunsaturated lipid comprises two or more species of the same fatty acid described herein or a derivative thereof, where the only difference between the various species is the number of deuterium at the bis-allylic and/or mono-allylic positions. For example, when the mixture comprise deuterated linolenic acid, it may comprises various species of linolenic acid, containing one to four deuterium atoms at the bis-allylic positions, such as the following species:




Similarly, when the mixtures comprises species of deuterated linoleic acid or derivative thereof, the mixtures may comprise combinations of various species of linoleic acid containing one or two deuterium atoms at the bis-allylic positions, or containing any one between one to six deuterium atoms at various bis-allylic and mono-allylic positions. When the mixture comprises species of deuterated arachidonic acid or derivative thereof, the mixtures may comprise combinations of various species of arachidonic acid containing one to six deuterium atoms at the bis-allylic positions, or containing any one between one to ten deuterium atoms at various bis-allylic and mono-allylic positions. When the mixture comprises species of deuterated eicosapentaenoic acid or derivative thereof, the mixtures may comprise combinations of various species of eicosapentaenoic acid containing one to eight deuterium atoms at the bis-allylic positions, or containing any one between one to twelve deuterium atoms at various bis-allylic and mono-allylic positions. When the mixture comprises species of deuterated docosahexaenoic acid or derivative thereof, the mixtures may comprise combinations of various species of docosahexaenoic acid containing one to ten deuterium atoms at the bis-allylic positions, or containing any one between one to fourteen deuterium atoms at various bis-allylic and mono-allylic positions. In some further embodiments, the method yields deuterated product predominantly at the bis-allylic positions. In some such embodiments, the method yields deuterated products with less than 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1% degree of deuteration at the mono-allylic positions.
In some embodiments of the mixture of polyunsaturated lipids described herein, the method yields a mixture has a degree of deuteration of at least 50% after the reaction is completed, for example, at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% at the bis-allylic positions. In some further embodiments, the degree of deuteration is at least 70% at the bis-allylic positions. The term “deuteration degree”, “degree of deuteration” of “level of deuteration” as used herein, refer to the percentage of deuterium atoms at the bis-allylic positions and/or mono-allylic positions of a compound as compared to the same compound without deuteration. It may be calculated as following:

degree of deuteration (%) at bis-allylic position=the number of deuterium atoms at the bis-allylic positions of a compound/the total number of hydrogen and deuterium atoms at the bis-allylic positions of the compound

For a mixture containing deuterated compounds with various degree of deuteration (e.g., a mixture containing equal amount of Compounds A and B, having degree of deuteration of 33.3% and 66.7% respectively), the total or combined degree of deuteration of the mixture may be calculated as the following:

molar percentage of Compound A*degree of deuteration of Compound A+molar percentage of Compound B*degree of deuteration of Compound B 

For example, if the product mixture contains the following three compounds in equal molar amount:




then the degree of deuteration at the bis-allylic position is 66.7%. A more practical way to determine the total percentage of deuteration is to rely on proton-carbon 13NMR bis-allylic peak integration measurements and mass-spectrometric methods.

Compositions

Some embodiments relate to a composition comprising one or more isotopically modified polyunsaturated lipids having an isotope predominantly at one or more bis-allylic positions, wherein the isotopically modified polyunsaturated lipids are prepared by a method of any one of claims. In some embodiments, the isotope is deuterium. In some embodiments, the isotope is tritium.
In some embodiments, the isotopically-modified polyunsaturated lipids in the composition described herein are deuterated predominantly at bis-allylic sites. In some embodiments, the composition described herein contains polyunsaturated lipid having two or more carbon-carbon double bonds. In some embodiments, the composition described herein contains polyunsaturated lipid having three or more carbon-carbon double bonds.
One will readily appreciate that when one of the two hydrogens of a methylene group is replaced with a deuterium atom, the resultant compound may possess a stereocenter. In some embodiments, it may be desirable to use racemic compounds. In other embodiments, it may be desirable to use enantiomerically pure compounds. In additional embodiments, it may be desirable to use diastereomerically pure compounds. In some embodiments, it may be desirable to use mixtures of compounds having enantiomeric excesses and/or diastereomeric excesses of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 65%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100%, or a range bounded by any two of the aforementioned percentages. In some embodiments, it may be preferable to utilize stereochemically pure enantiomers and/or diastereomers of embodiments—such as when enzymatic reactions or contacts with chiral molecules are being targeted for attenuating oxidative damage. However, in many circumstances, non-enzymatic processes and/or non-chiral molecules are being targeted for attenuating oxidative damage. In such circumstances, embodiments may be utilized without concern for their stereochemical purity. Moreover, in some embodiments, mixtures of enantiomers and diastereomers may be used even when the compounds are targeting enzymatic reactions and/or chiral molecules for attenuating oxidative damage.
In some aspects, isotopically modified compounds impart an amount of heavy atoms in a particular tissue upon administration. Thus, in some aspects, the amount of heavy molecules will be a particular percentage of the same type of molecules in a tissue. For example, the percentage of heavy molecules may be about at least 0.001%, 0.005%, 0.1%, 1%, 10%, 20%, 30%, 40%, or 50% of the same type of molecules (i.e., natural as opposed to isotopically modified) in a tissue.
Uses of Methods of Treatments
Some embodiments provide a method of treating, ameliorating or preventing a disease or condition associated with lipid peroxidation or lipid autooxidation in a subject in need thereof, comprising administering an effective amount of one or more isotopically modified polyunsaturated lipids described herein, or pharmaceutically acceptable salts thereof to the subject. In some embodiments, the isotopically modified polyunsaturated lipid(s) administered comprises from about 1% to about 99%, from about 1% to about 10%, or from about 1% to about 5% of the total amount of fats, fatty acids, and fatty acid esters administered to, or ingested by, the subject. In some further embodiments, the one or more isotopically modified polyunsaturated lipids described herein, or a pharmaceutically acceptable salt thereof, comprises less than about 5%, less than about 2%, or less than about 1% of the total amount of fats, fatty acids, and fatty acid esters administered to, or ingested by, the subject.
In some embodiments described herein, the disease or condition is a neurological condition or a neurodegenerative condition. In some further embodiments, the neurological condition is Alzheimer's disease, Parkinson's disease, Mild Cognitive Impairment (MCI), Frontotemperal Dementia, Amyotrophic Lateral Sclerosis (ALS), an ataxia (e.g., Friedreich's ataxia), Down syndrome, epilepsy, Huntington's disease, infantile neuroaxonal dystrophy (INAD), Alpers disease, schizophrenia, Wilson's disease, neurodegeneration with brain iron accumulation (NBIA), progressive supranuclear palsy (PSP), multiple sclerosis, Creutzfeld-Jakob's disease, Duchenne muscular dystrophy, Smith-Lemli-Opitz syndrome (SLOS), Rett syndrome, Gaucher Type 2, or Angelman syndrome. In some other embodiments, the neurodegenerative disease or condition related to tauopathy, for example, argyrophilic grain disease (AGD), chronic traumatic encephalopathy (CTE), corticobasal degeneration (CBD), frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), ganglioglioma, gangliocytoma, lipofuscinosis, lytico-bodig disease, meningioangiomatosis, pantothenate kinase-associated neurodegeneration (PKAN), Pick's disease, postencephalitic parkinsonism, primary age-related tauopathy (PART), Steele-Richardson-Olszewski syndrome (SROS), and subacute sclerosing panencephalitis (SSPE).
In some embodiments described herein, the disease or condition is a lysosomal storage disorder. In some further embodiments, the lysosomal storage disorder is Batten disease, Nieman-Pick disease, Tay-Sachs disease, Sandhoff disease, or ataxia with vitamin E deficiency (AVED).
In some embodiments described herein, the disease or condition is a retinal condition. In some further embodiments, the retinal condition is retinitis pigmentosa, age-related macular degeneration, cataracts, diabetic retinopathy, Leber's hereditary optic neuropathy (LHON), Leber's congenital amaurosis, macular telangiectasia, Stargardt disease, glaucoma, optic neuropathies, or opthalmoplegias.
In some embodiments described herein, the disease or condition is pain. In some further embodiments, the pain is acute pain; neurogenic inflammation; chronic pain; dynamic, mechanical or thermal allodynia (pain that results from a stimulus that is not normally painful); or increased response to painful stimuli (such as hyperalgesia, fibromyalgia, and activation of the TRPA1 receptor).
In some embodiments described herein, the disease or condition is a sleep disorder. Non-limiting examples of the sleep disorder may include lifestyle related sleep deficiency; alcohol related sleep deficiency; idiopathic hypersomnia; narcolepsy; various sleep apneas; various parasomnias; restless leg syndrome; sleep state misperception; mood disorders such as depression; anxiety disorders; panic; psychoses such as schizophrenia; as well as circadian rhythm related sleep disorders, including jetlag related disorders and nightshift associated conditions.
In some embodiments described herein, the disease or condition is an impaired energy processing disorder or mitochondrial deficiency, for example: co-enzyme Q deficiency; mitochondrial complex IV deficiency; diabetes mellitus and deafness (DAD); Maternally Inherited Diabetes and Deafness (MIDD); Barth syndrome; Leigh syndrome; Kearns-Sayre syndrome (KSS); mitochondrial myopathy; mitochondrial encephalopathy; lactic acidosis; stroke-like episodes (MELAS); mitochondrial neurogastrointestinal encephalomyopathy (MNGIE); myoclonus epilepsy associated with ragged-red fibers (MERRF) syndrome; myoneurogenetic gastrointestinal encephalopathy (MNGIE) and neuropathy; Wolff-Parkinson-White syndrome and other cardiomyopathies; X-linked adrenoleukodystrophy (X-ALD), as well as diseases of musculoskeletal system (lipid myopathies, chronic fatigue, fibromyalgia syndrome); kidney (Fanconi's syndrome and glomerulonephropathies); blood (Pearson's syndrome, aceruloplasminemia, or sideroblastic anemia), and brain (migraines, seizures, and strokes).
In some embodiments described herein, the disease or condition is a hepatic disorder. Non-limiting examples of hepatic disorders include alcoholic fatty liver disease, nonalcoholic fatty liver disease, steatohepatitis, cirrhosis, hepatocellular carcinoma, obstructive jaundice, cholelitiasis, or a biliary tract disease.
In some embodiments described herein, the disease or condition is lipidemia or cardiac-related conditions, such as liporegulation disorders, lipotoxicity, ischemic heart disease, hypertension, atrial fibrillation, left ventricular hypertrophy, coronary artery disease, or atherosclerosis.
In some further embodiments, at least certain amount of the isotopically modified polyunsaturated lipid is incorporated into the subject's body following administration such that the incorporated compounds in the patient's body is sufficient to reduce or prevent lipid autooxidation of the natural (undeuterated) polyunsaturated fatty acid or ester in the subject's body. In some embodiments, the method also reduces ferroptosis.
Pharmaceutical Compositions
Some embodiments include pharmaceutical compositions comprising: (a) an effective amount of one or more isotopically modified polyunsaturated lipids described herein, or a pharmaceutically acceptable salt thereof; and (b) a pharmaceutically acceptable carrier, diluent, excipient or combination thereof. In some embodiments, the polyunsaturated lipid is 11,11-D2-linoleic acid or an ester thereof. In one particular embodiment, the polyunsaturated lipid is 11,11-D2-linoleic acid ethyl ester.
It is also contemplated that it may be useful to formulate the polyunsaturated lipid as a salt form. For example, the use of salt formation as a means of tailoring the properties of pharmaceutical compounds is well known. See Stahl et al., Handbook of pharmaceutical salts: Properties, selection and use (2002) Weinheim/Zurich: Wiley-VCH/VHCA; Gould, Salt selection for basic drugs, Int. J. Pharm. (1986), 33:201-217. Salt formation can be used to increase or decrease solubility, to improve stability or toxicity, and to reduce hygroscopicity of a drug product.
Formulation of polyunsaturated lipid(s) as salt(s) includes, but is not limited to, the use of basic inorganic salt forming agents, basic organic salt forming agents, and salt forming agents containing both acidic and basic functional groups. Various useful inorganic bases for forming salts include, but are not limited to, alkali metal salts such as salts of lithium, sodium, potassium rubidium, cesium, and francium, and alkaline earth metal salts such as berylium, magnesium, calcium, strontium, barium, and radium, and metals such as aluminum. These inorganic bases may further include counterions such as carbonates, hydrogen carbonates, sulfates, hydrogen sulfates, sulfites, hydrogen sulfites, phosphates, hydrogen phosphates, dihydrogen phosphates, phosphites, hydrogen phosphites, hydroxides, oxides, sulfides, alkoxides such as methoxide, ethoxide, and t-butoxide, and the like. Various useful organic bases for forming salts include, but are not limited to, amino acids, basic amino acids such as arginine, lysine, ornithine and the like, ammonia, alkylamines such as methylamine, ethylamine, dimethylamine, diethylamine, trimethylamine, triethylamine and the like, heterocyclic amines such as pyridine, picoline and the like, alkanolamines such as ethanolamine, diethanolamine, triethanolamine and the like, diethylaminoethanol, dimethylaminoethanol, N-methylglucamine, dicyclohexylamine, N,N′-dibenzylethylenediamine, ethylenediamine, piperazine, choline, trolamine, imidazole, diolamine, betaine, tromethamine, meglumine, chloroprocain, procaine, and the like.
Pharmaceutically acceptable salts are well known in the art and include many of the above-recited inorganic and organic bases. Pharmaceutically acceptable salts further include salts and salt-forming agents found in drugs approved by the Food and Drug Administration and foreign regulatory agencies. Pharmaceutically acceptable organic cations for incorporation include, but are not limited to, benzathine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine, procaine, benethamine, clemizole, diethylamine, piperazine, and tromethamine. Pharmaceutically acceptable metallic cations for incorporation include, but are not limited to, aluminum, calcium, lithium, magnesium, potassium, sodium, zinc, barium, and bismuth. Additional salt-forming agents include, but are not limited to, arginine, betaine, carnitine, diethylamine, L-glutamine, 2-(4-imidazolyl)ethylamine, isobutanolamine, lysine, N-methylpiperazine, morpholine, and theobromine.
In addition to the selected compound useful as described above, some embodiments include compositions containing a pharmaceutically-acceptable carrier. The term “pharmaceutically-acceptable carrier”, as used herein, means one or more compatible solid or liquid filler diluents or encapsulating substances, which are suitable for administration to a mammal. The term “compatible”, as used herein, means that the components of the composition are capable of being commingled with the subject compound, and with each other, in a manner such that there is no interaction, which would substantially reduce the pharmaceutical efficacy of the composition under ordinary use situations. Pharmaceutically-acceptable carriers must, of course, be of sufficiently high purity and sufficiently low toxicity to render them suitable for administration preferably to an animal, preferably mammal being treated.
Pharmaceutically-acceptable carriers include, for example, solid or liquid fillers, diluents, hydrotropies, surface-active agents, and encapsulating substances. Some examples of substances, which can serve as pharmaceutically-acceptable carriers or components thereof, are sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose, and methyl cellulose; powdered tragacanth; malt; gelatin; talc; solid lubricants, such as stearic acid and magnesium stearate; calcium sulfate; vegetable oils, such as peanut oil, cottonseed oil, sesame oil, olive oil, corn oil and oil of theobroma; polyols such as propylene glycol, glycerine, sorbitol, mannitol, and polyethylene glycol; alginic acid; emulsifiers, such as the TWEENS; wetting agents, such sodium lauryl sulfate; coloring agents; flavoring agents; tableting agents, stabilizers; antioxidants; preservatives; pyrogen-free water; isotonic saline; and phosphate buffer solutions.
Optional pharmaceutically-active materials may be included, which do not substantially interfere with the inhibitory activity of the compound. The amount of carrier employed in conjunction with the compound is sufficient to provide a practical quantity of material for administration per unit dose of the compound. Techniques and compositions for making dosage forms useful in the methods described herein are described in the following references, all incorporated by reference herein: Modern Pharmaceutics, 4th Ed., Chapters 9 and 10 (Banker & Rhodes, editors, 2002); Lieberman et al., Pharmaceutical Dosage Forms: Tablets (1989); and Ansel, Introduction to Pharmaceutical Dosage Forms 8th Edition (2004).
Various oral dosage forms can be used, including such solid forms as tablets, capsules, granules and bulk powders. Tablets can be compressed, tablet triturates, enteric-coated, sugar-coated, film-coated, or multiple-compressed, containing suitable binders, lubricants, diluents, disintegrating agents, coloring agents, flavoring agents, flow-inducing agents, and melting agents. Liquid oral dosage forms include aqueous solutions, emulsions, suspensions, solutions and/or suspensions reconstituted from non-effervescent granules, and effervescent preparations reconstituted from effervescent granules, containing suitable solvents, preservatives, emulsifying agents, suspending agents, diluents, sweeteners, melting agents, coloring agents and flavoring agents.
The pharmaceutically-acceptable carriers suitable for the preparation of unit dosage forms for peroral administration is well-known in the art. Tablets typically comprise conventional pharmaceutically-compatible adjuvants as inert diluents, such as calcium carbonate, sodium carbonate, mannitol, lactose and cellulose; binders such as starch, gelatin and sucrose; disintegrants such as starch, alginic acid and croscarmelose; lubricants such as magnesium stearate, stearic acid and talc. Glidants such as silicon dioxide can be used to improve flow characteristics of the powder mixture. Coloring agents, such as the FD&C dyes, can be added for appearance. Sweeteners and flavoring agents, such as aspartame, saccharin, menthol, peppermint, and fruit flavors, are useful adjuvants for chewable tablets. Capsules typically comprise one or more solid diluents disclosed above. The selection of carrier components depends on secondary considerations like taste, cost, and shelf stability, which are not critical, and can be readily made by a person skilled in the art.
Per-oral compositions also include liquid solutions, emulsions, suspensions, and the like. The pharmaceutically-acceptable carriers suitable for preparation of such compositions are well known in the art. Typical components of carriers for syrups, elixirs, emulsions and suspensions include ethanol, glycerol, propylene glycol, polyethylene glycol, liquid sucrose, sorbitol and water. For a suspension, typical suspending agents include methyl cellulose, sodium carboxymethyl cellulose, AVICEL RC-591, tragacanth and sodium alginate; typical wetting agents include lecithin and polysorbate 80; and typical preservatives include methyl paraben and sodium benzoate. Peroral liquid compositions may also contain one or more components such as sweeteners, flavoring agents and colorants disclosed above.
Such compositions may also be coated by conventional methods, typically with pH or time-dependent coatings, such that the subject compound is released in the gastrointestinal tract in the vicinity of the desired topical application, or at various times to extend the desired action. Such dosage forms typically include, but are not limited to, one or more of cellulose acetate phthalate, polyvinylacetate phthalate, hydroxypropyl methyl cellulose phthalate, ethyl cellulose, Eudragit coatings, waxes and shellac.
Compositions described herein may optionally include other drug actives or supplements. For example, the pharmaceutical composition is administered concomitantly with one or more antioxidants. In some embodiments, the antioxidant is selected from the group consisting of Coenzyme Q, idebenone, mitoquinone, mitoquinol, vitamin E, and vitamin C, and combinations thereof. In some such embodiments, at least one antioxidant may be taken concurrently, prior to, or subsequent to the administration of 11,11-D2-linoleic acid or the ester thereof. In some embodiments, the antioxidant and 11,11-D2-linoleic acid or the ester thereof may be in a single dosage form. In some embodiments, the single dosage form is selected from the group consisting of a pill, a tablet, and a capsule.
It will be understood by those of skill in the art that numerous and various modifications can be made without departing from the spirit of the present invention. Therefore, it should be clearly understood that the embodiments of the present invention disclosed herein are illustrative only and are not intended to limit the scope of the present invention. Any reference referred to herein is incorporated by reference for the material discussed herein, and in its entirety.
Co-Administration
In some embodiments, the polyunsaturated lipid(s) disclosed herein are administered in combination with one or more antioxidants.
Although antioxidants cannot cancel the negative effects of PUFA peroxidation due to the stochastic nature of the process and the stability of PUFA peroxidation products (reactive carbonyls) to antioxidant treatment, co-administration of antioxidants with compositions resistant to oxidation, such as those described herein, may prove beneficial for treating oxidative stress-related disorders.
Certain antioxidants contemplated as useful for co-administration include the following: vitamins, such as vitamin C and vitamin E; glutathione, lipoic acid, uric acid, carotenes, lycopene, lutein, anthocyanins, oxalic acid, phytic acid, tannins, coenzyme Q, melatonin, tocopherols, tocotrienols, polyphenols including resveratrol, flavonoids, selenium, eugenol, idebenone, mitoquinone, mitoquinol, ubiquinone, Szeto-S chiller peptides, and mitochondrial-targeted antioxidants. When not explicitly mentioned, quinone derivatives of the aforementioned antioxidants are also contemplated as useful for co-administration.
Kits
Some additional embodiments of the present disclosure relate to kits comprising a pharmaceutical composition, prescribing information, and a container, wherein the pharmaceutical composition comprises a therapeutically effective amount of one or more isotopically modified polyunsaturated lipids described herein. In some embodiments, isotopically modified polyunsaturated lipid is a deuterated polyunsaturated acid (PUFA) or an ester, thioester, amide, phosphate, or other prodrug thereof (such as a phospholipid derivative). In some further embodiment, the deuterated PUFA is 11,11-D2-linoleic acid and/or an ester thereof. In one particular embodiment, the isotopically modified PUFA is 11,11-D2-linoleic acid ethyl ester. In some embodiments, the prescribing information advises a subject to take the pharmaceutical composition with food, or take the pharmaceutical composition between meals. The kit may include one or more unit dosage forms comprising 11,11-D2-linoleic acid or the ester thereof. The unit dosage forms may be of an oral formulation. For example, the unit dosage forms may comprise pills, tablets, or capsules. The kit may include a plurality of unit dosage forms. In some embodiments, the unit dosage forms are in a container. In some embodiments, the dosage forms are single oral dosage forms comprising 11,11-D2-linoleic acid or the ester thereof, e.g., the ethyl ester.
The methods, compositions and kits disclosed herein may include information. The information may be in a form prescribed by a governmental agency regulating the manufacture, use, or sale of pharmaceuticals, which notice is reflective of approval by the agency of the form of the drug for human or veterinary administration. Such information, for example, may be the labeling approved by the U.S. Food and Drug Administration for prescription drugs, or the approved product insert. The information can include required information regarding dose and dosage forms, administration schedules and routes of administration, adverse events, contraindications, warning and precautions, drug interactions, and use in specific populations (see, e.g., 21 C.F.R. § 201.57 which is incorporated herein by reference in its entirety), and in some embodiments is required to be present on or associated with the drug for sale of the drug. In some embodiments, a kit is for sale of a prescription drug requiring the approval of and subject to the regulations of a governmental agency, such as the Food and Drug Administration of the United States. In some embodiments, the kit comprises the label or product insert required by the agency, such as the FDA, for sale of the kit to consumers, for example in the U.S. In preferred embodiments, the information instructs an individual to take 11,11-D2-linoleic acid or the ester thereof between meals, or with food, in order to reduce possible adverse event(s), for example gastrointestinal adverse event(s).
Instructions and/or information may be present in a variety of forms, including printed information on a suitable medium or substrate (e.g., a piece or pieces of paper on which the information is printed), computer readable medium (e.g., diskette, CD, etc. on which the information has been recorded), or a website address that may be accessed via the internet. Printed information may, for example, be provided on a label associated with a drug product, on the container for a drug product, packaged with a drug product, or separately given to the patient apart from a drug product, or provided in manner that the patient can independently obtain the information (e.g., a website). Printed information may also be provided to a medical caregiver involved in treatment of the patient. In some embodiments, the information is provided to a person orally.
Some embodiments comprise a therapeutic package suitable for commercial sale. Some embodiments comprise a container. The container can be in any conventional shape or form as known in the art which is made of a pharmaceutically acceptable material, for example a paper or cardboard box, a glass or plastic bottle or jar, a re-sealable bag (e.g., to hold a “refill” of tablets for placement into a different container), or a blister pack with individual dosages for pressing out of the pack according to a therapeutic schedule. The container employed can depend on the exact dosage form involved, e.g., a conventional cardboard box would not generally be used to hold a liquid suspension. It is feasible that more than one container can be used together in a single package to market a single dosage form. For example, tablets may be contained in a bottle which is in turn contained within a box.
The information can be associated with the container, for example, by being:
written on a label (e.g., the prescription label or a separate label) adhesively affixed to a bottle containing a dosage form described herein; included inside a container as a written package insert, such as inside a box which contains unit dose packets; applied directly to the container such as being printed on the wall of a box; or attached as by being tied or taped, e.g., as an instructional card affixed to the neck of a bottle via a string, cord or other line, lanyard or tether type device. The information may be printed directly on a unit dose pack or blister pack or blister card.

## Claims

1. A ruthenium complex of the formula I:



wherein R and R′ comprise the remainder of linolenic acid, arachidonic acid, eicosapentaenoic acid, or docosahexaenoic acid or an ester thereof; wherein when present, the ester is a C1-C21 alkyl ester;
wherein the ruthenium complex further comprises a cyclopentadiene (Cp) ligand; and
wherein ruthenium in the ruthenium complex is in the +1 oxidation state (Ru+1).

2. The complex of claim 1, wherein R and R′ comprise the remainder of linolenic acid.

3. The complex of claim 1, wherein R and R′ comprise the remainder of arachidonic acid.

4. The complex of claim 1, wherein R and R′ comprise the remainder of eicosapentaenoic acid.

5. The complex of claim 1, wherein R and R′ comprise the remainder of docosahexaenoic acid.

6. The complex of claim 1, wherein R and R′ comprise the remainder of linolenic C1-C21 alkyl ester.

7. The complex of claim 1, wherein R and R′ comprise the remainder of arachidonic C1-C21 alkyl ester.

8. The complex of claim 1, wherein R and R′ comprise the remainder of eicosapentaenoic C1-C21 alkyl ester.

9. The complex of claim 1, wherein R and R′ comprise the remainder of docosahexaenoic C1-C21 alkyl ester.

10. The complex of claim 1, wherein R and R′ comprise the remainder of linolenic ethyl ester.

11. The complex of claim 1, wherein R and R′ comprise the remainder of arachidonic ethyl ester.

12. The complex of claim 1, wherein R and R′ comprise the remainder of eicosapentaenoic ethyl ester.

13. The complex of claim 1, wherein R and R′ comprise the remainder of docosahexaenoic ethyl ester.