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          Labs @ NIAMS
          Office of Science and Technology
          Genomic Technology Section








Genomic Technology Section


Stefania Dell'Orso, Ph.D. FacebookEmailPrintFacebookEmail
        Genomic Technology Unit Banner

      Overview

                                  Staff Scientist
      
  


Stefania Dell'Orso, Ph.D.
      
  


            
      
            dellorsos@mail.nih.gov
                                
            The mission of the Genomic Technology Section is to help NIAMS investigators solve highly diverse biological and biomedical research questions by providing a diverse portfolio of sequencing services as well as consultation and training for researchers with specific needs or challenging problems. The core is a state-of-the-art laboratory facility equipped with the latest DNA sequencing and library preparation technologies. A wide array of sample preparation methods enable the use of diverse applications, including Whole-genome sequencing, RNA-Seq, small RNA discovery, methylation profiling, genome-wide protein-DNA interaction analysis (ChIP-Seq) and ATAC-Seq, exome sequencing, amplicon sequencing, Single Cells RNA-seq, Single Cells ATAC-Seq and Single Cell western.
      

                                            In the Lab with NIAMS
      
  

      Learn about the Genomic Technology Section, a Core Facility in the institute’s Intramural Research Program. This facility helps investigators solve highly diverse biological and biomedical research questions by providing a diverse portfolio of sequencing services as well as consultation and training for researchers with specific needs or challenging problems.

                                                      Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Biodata Mining and Discovery
	Flow Cytometry
	Genomic Technology
	Laboratory Animal Care and Use
	Light Imaging
	Translational Immunology

                                    Staff
                            
            **Stefania Dell'Orso, Ph.D.**Staff Scientistdellorsos@mail.nih.gov
    **Gustavo Gutierrez-Cruz, M.S.**Research Assistantgutierrg@mail.nih.gov
    **Shamima Islam, M.B.B.S., Ph.D.**Biologistshamima.islam@nih.gov
    **Faiza Naz, M.S.**Research Assistantfaiza.naz@nih.gov
    **Gustaf Wigerblad, Ph.D.**Research Fellowcarl.wigerblad@nih.gov
            
                                    
                        
                        
                                    Scientific Publications
              Selected Recent Publications
                  
      Single-Cell Analysis Reveals the Range of Transcriptional States of Circulating Human Neutrophils.

    Wigerblad G, Cao Q, Brooks S, Naz F, Gadkari M, Jiang K, Gupta S, O'Neil L, Dell'Orso S, Kaplan MJ, Franco LM
        

                    J Immunol.
        
                    2022 Aug 15;
        
                    209(4).
        
                    doi: 10.4049/jimmunol.2200154
                            PMID: 35858733
              Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease.

    Beck DB, Ferrada MA, Sikora KA, Ombrello AK, Collins JC, Pei W, Balanda N, Ross DL, Ospina Cardona D, Wu Z, Patel B, Manthiram K, Groarke EM, Gutierrez-Rodrigues F, Hoffmann P, Rosenzweig S, Nakabo S, Dillon LW, Hourigan CS, Tsai WL, Gupta S, Carmona-Rivera C, Asmar AJ, Xu L, Oda H, Goodspeed W, Barron KS, Nehrebecky M, Jones A, Laird RS, Deuitch N, Rowczenio D, Rominger E, Wells KV, Lee CR, Wang W, Trick M, Mullikin J, Wigerblad G, Brooks S, Dell'Orso S, Deng Z, Chae JJ, Dulau-Florea A, Malicdan MCV, Novacic D, Colbert RA, Kaplan MJ, Gadina M, Savic S, Lachmann HJ, Abu-Asab M, Solomon BD, Retterer K, Gahl WA, Burgess SM, Aksentijevich I, Young NS, Calvo KR, Werner A, Kastner DL, Grayson PC
        

                    N Engl J Med.
        
                    2020 Dec 31;
        
                    383(27).
        
                    doi: 10.1056/NEJMoa2026834
                            PMID: 33108101
              FoxO maintains a genuine muscle stem-cell quiescent state until geriatric age.

    García-Prat L, Perdiguero E, Alonso-Martín S, Dell'Orso S, Ravichandran S, Brooks SR, Juan AH, Campanario S, Jiang K, Hong X, Ortet L, Ruiz-Bonilla V, Flández M, Moiseeva V, Rebollo E, Jardí M, Sun HW, Musarò A, Sandri M, Del Sol A, Sartorelli V, Muñoz-Cánoves P
        

                    Nat Cell Biol.
        
                    2020 Nov;
        
                    22(11).
        
                    doi: 10.1038/s41556-020-00593-7
                            PMID: 33106654
              Monocyte Subsets With High Osteoclastogenic Potential and Their Epigenetic Regulation Orchestrated by IRF8.

    Das A, Wang X, Kang J, Coulter A, Shetty AC, Bachu M, Brooks SR, Dell'Orso S, Foster BL, Fan X, Ozato K, Somerman MJ, Thumbigere-Math V
        

                    J Bone Miner Res.
        
                    2021 Jan;
        
                    36(1).
        
                    doi: 10.1002/jbmr.4165
                            PMID: 32804442
              Sex differences in neutrophil biology modulate response to type I interferons and immunometabolism.

    Gupta S, Nakabo S, Blanco LP, O'Neil LJ, Wigerblad G, Goel RR, Mistry P, Jiang K, Carmona-Rivera C, Chan DW, Wang X, Pedersen HL, Gadkari M, Howe KN, Naz F, Dell'Orso S, Hasni SA, Dempsey C, Buscetta A, Frischmeyer-Guerrerio PA, Kruszka P, Muenke M, Franco LM, Sun HW, Kaplan MJ
        

                    Proc Natl Acad Sci U S A.
        
                    2020 Jul 14;
        
                    117(28).
        
                    doi: 10.1073/pnas.2003603117
                            PMID: 32601182
              JAK Inhibition Differentially Affects NK Cell and ILC1 Homeostasis.

    Vian L, Le MT, Gazaniga N, Kieltyka J, Liu C, Pietropaolo G, Dell'Orso S, Brooks SR, Furumoto Y, Thomas CJ, O'Shea JJ, Sciumè G, Gadina M
        

                    Front Immunol.
        
                    2019;
        
                    10().
        
                    doi: 10.3389/fimmu.2019.02972
                            PMID: 31921209
              Transcriptomic, epigenetic, and functional analyses implicate neutrophil diversity in the pathogenesis of systemic lupus erythematosus.

    Mistry P, Nakabo S, O'Neil L, Goel RR, Jiang K, Carmona-Rivera C, Gupta S, Chan DW, Carlucci PM, Wang X, Naz F, Manna Z, Dey A, Mehta NN, Hasni S, Dell'Orso S, Gutierrez-Cruz G, Sun HW, Kaplan MJ
        

                    Proc Natl Acad Sci U S A.
        
                    2019 Dec 10;
        
                    116(50).
        
                    doi: 10.1073/pnas.1908576116
                            PMID: 31754025
              Correction: Single cell analysis of adult mouse skeletal muscle stem cells in homeostatic and regenerative conditions (doi: 10.1242/dev.174177).

    Dell'Orso S, Juan AH, Ko KD, Naz F, Perovanovic J, Gutierrez-Cruz G, Feng X, Sartorelli V
        

                    Development.
        
                    2019 Jun 28;
        
                    146(13).
        
                    doi: 10.1242/dev.181743
                            PMID: 31253633
              miR-155 harnesses Phf19 to potentiate cancer immunotherapy through epigenetic reprogramming of CD8(+) T cell fate.

    Ji Y, Fioravanti J, Zhu W, Wang H, Wu T, Hu J, Lacey NE, Gautam S, Le Gall JB, Yang X, Hocker JD, Escobar TM, He S, Dell'Orso S, Hawk NV, Kapoor V, Telford WG, Di Croce L, Muljo SA, Zhang Y, Sartorelli V, Gattinoni L
        

                    Nat Commun.
        
                    2019 May 14;
        
                    10(1).
        
                    doi: 10.1038/s41467-019-09882-8
                            PMID: 31089138
              Single cell analysis of adult mouse skeletal muscle stem cells in homeostatic and regenerative conditions.

    Dell'Orso S, Juan AH, Ko KD, Naz F, Perovanovic J, Gutierrez-Cruz G, Feng X, Sartorelli V
        

                    Development.
        
                    2019 Apr 11;
        
                    146(12).
        
                    doi: 10.1242/dev.174177
                            PMID: 30890574
              Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus.

    Liu Y, Lightfoot YL, Seto N, Carmona-Rivera C, Moore E, Goel R, O'Neil L, Mistry P, Hoffmann V, Mondal S, Premnath PN, Gribbons K, Dell'Orso S, Jiang K, Thompson PR, Sun HW, Coonrod SA, Kaplan MJ
        

                    JCI Insight.
        
                    2018 Dec 6;
        
                    3(23).
        
                    pii: 124729. doi: 10.1172/jci.insight.124729
                            PMID: 30518690
              Dysregulated neutrophil responses and neutrophil extracellular trap formation and degradation in PAPA syndrome.

    Mistry P, Carmona-Rivera C, Ombrello AK, Hoffmann P, Seto NL, Jones A, Stone DL, Naz F, Carlucci P, Dell'Orso S, Gutierrez-Cruz G, Sun HW, Kastner DL, Aksentijevich I, Kaplan MJ
        

                    Ann Rheum Dis.
        
                    2018 Dec;
        
                    77(12).
        
                    doi: 10.1136/annrheumdis-2018-213746
                            PMID: 30131320
              A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans.

    Tsai PF, Dell'Orso S, Rodriguez J, Vivanco KO, Ko KD, Jiang K, Juan AH, Sarshad AA, Vian L, Tran M, Wangsa D, Wang AH, Perovanovic J, Anastasakis D, Ralston E, Ried T, Sun HW, Hafner M, Larson DR, Sartorelli V
        

                    Mol Cell.
        
                    2018 Jul 5;
        
                    71(1).
        
                    doi: 10.1016/j.molcel.2018.06.008
                            PMID: 29979962
              Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies.

    Feng X, Naz F, Juan AH, Dell'Orso S, Sartorelli V
        

                    J Vis Exp.
        
                    2018 Apr 19;
        
                    (134).
        
                    doi: 10.3791/57212
                            PMID: 29733324
              Neutrophil subsets and their gene signature associate with vascular inflammation and coronary atherosclerosis in lupus.

    Carlucci PM, Purmalek MM, Dey AK, Temesgen-Oyelakin Y, Sakhardande S, Joshi AA, Lerman JB, Fike A, Davis M, Chung JH, Playford MP, Naqi M, Mistry P, Gutierrez-Cruz G, Dell'Orso S, Naz F, Salahuddin T, Natarajan B, Manna Z, Tsai WL, Gupta S, Grayson P, Teague H, Chen MY, Sun HW, Hasni S, Mehta NN, Kaplan MJ
        

                    JCI Insight.
        
                    2018 Apr 19;
        
                    3(8).
        
                    pii: 99276. doi: 10.1172/jci.insight.99276
                            PMID: 29669944
            
                                    News & Highlights
              
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