Exhibit 31.1

Certification of Principal Executive Officer

Pursuant to Rule 13a-14(a) of

Securities Exchange Act of 1934

I,
William P. Hales, certify that:



|1. | I have reviewed this annual report on Form 10-KSB of Hemagen Diagnostics, Inc.;
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|2. | Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements
made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;
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|3. | Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material respects the financial
condition, results of operations and cash flows of the small business issuer as of, and for, the periods presented in this report;
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|4. | The small business issuers other certifying officer and I are responsible for establishing and maintaining disclosure controls and procedures (as
defined in Exchange Act Rules 13a-15(e) and 15d-15(e) for the small business issuer and we have:
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| | a. | Designed such disclosure controls and procedures or caused such disclosure controls and procedures to be designed under our supervision, to ensure that
material information relating to the registrant, including its consolidated subsidiaries, is made known to us by others within those entities, particularly during the period in which this report is being prepared;
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| | b. | Evaluated the effectiveness of the small business issuers disclosure controls and procedures and presented in this report our conclusions about the
effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and
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| | c. | Disclosed in this report any change in the small business issuers internal control over financial reporting that occurred during the small business
issuers most recent fiscal quarter (the small business issuers fourth quarter in the case of the annual report) that has materially affected, or is reasonably likely to materially affect, the small business issuers internal control
over financial reporting; and
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|5. | I have disclosed, based on our most recent evaluation of internal control over financial reporting, to the small business issuers auditors and the audit
committee of small business issuers board of directors (or persons performing the equivalent functions):
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| | a. | All significant deficiencies and material weaknesses in the design or operation of internal control over financial reporting which are reasonably likely to
adversely affect the small business issuers ability to record, process, summarize and report financial information; and
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| | b. | Any fraud, whether or not material, that involves management or other employees who have a significant role in the small business issuers internal control
over financial reporting; and
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|                        |  |                                      
|----------------------- |  | -------------------------------------
|Date: December 31, 2007 |  | /s/ William P. Hales                 
|                        |  | William P. Hales                     
|                        |  | President and Chief Executive Officer