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                    Cryo-Electron Microscopy (Cryo-EM) Core, NIDDK

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        Cores & Support Services
        Advanced Light Microscopy & Image Analysis Core
                Clinical Laboratory Core
                Clinical Mass Spectrometry Core
                Computational Biomolecular Magnetic Resonance Core
                Cryo-Electron Microscopy (Cryo-EM) Core, NIDDK
                Cryo-Electron Microscopy (Cryo-EM) Core, Multi-Institute (MICEF)
                Genomics Core
                Human Energy & Body Weight Regulation Core
                Laboratory of Animal Sciences Section
                Mouse CRISPR/Cas9 Genome Editing Facility
                Mouse Metabolism CoreCryo-Electron Microscopy (Cryo-EM) Core, NIDDK
    **Jenny E. Hinshaw, Ph.D., Director
Yanxiang Cui, Ph.D., Technical DirectorBertram Canagarajah, Ph.D., Computational Director**
View staff and contact information.
The Cryo-Electron Microscopy (Cryo-EM) core provides the NIDDK scientific community with equipment and training in preparing and imaging negative stain and cryo-EM grids. The facility has three transmission electron microscopes, Glacios (K3 camera),  Tecnai TF20 (K2 camera) and a T12, all optimized for negative stain, cryo-EM Single Particle and Tomography imaging. The Glacio and TF20 are in Building 8, room B2A20-24 and the T12 is in Building 50, room B406 . The facility also has two EM grid plungers, a carbon evaporator, a glow discharge machine, and a rapid freezing device (Chameleon) located in building 8, room 1A18.
Automated image acquisition is set up on all scopes using the program SerialEM. The data is stored on LCBB, a server located in the Democracy NIDDK IT facility. There is minimal fee for service of the facility.
Contact Yanxiang Cui to discuss potential projects and to schedule time on the instruments.
Photo of Dr. Cui next to the NIDDK Glacios cryo-EM microscope.Dr. Yanxiang Cui, NIDDK CRYOEM core director, in front of the NIDDK Glacios cryo-EM microscope
Photo of the Vitrobot.Vitrobot used for freezing cryo-EM samples
        Last Reviewed June 2023 Contact Us
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