# Patent

## Title
Human G protein-coupled receptors

## Abstract

The invention disclosed in this patent document relates to transmembrane receptors, more particularly to endogenous, human orphan G protein-coupled receptors.

## Background
This application is a continuation of Ser. No. 10/393,807 filed Mar. 21, 2003, now U.S. Pat. No. 7,108,991, which is a continuation of Ser. No. 10/272,983, filed Oct. 17, 2002, which is a continuation of Ser. No. 09/417,044, filed Oct. 12, 1999, and claims priority benefit of Provisional Application Ser. No. 60/121,852 filed Feb. 26, 1999, Ser. No. 60/109,213 filed Nov. 20, 1998, Ser. No. 60/120,416 filed Feb. 16, 1999, Ser. No. 60/123,946 filed Mar. 12, 1999, Ser. No. 60/123,949 filed Mar. 12, 1999, Ser. No. 60/136,436 filed May 28, 1999, Ser. No. 60/136,439 filed May 28, 1999, Ser. No. 60/136,567 file May 28, 1999, Ser. No. 60/137,127 filed May 28, 1999, Ser. No. 60/137,131 filed May 28, 1999, Ser. No. 60/141,448 filed Jun. 29, 1999, Ser. No. 60/136,437 filed May 28, 1999, Ser. No. 60/156,653 filed Sep. 29, 1999, Ser. No. 60/156,633 filed Sep. 29, 1999, Ser. No. 60/156,555 filed Sep. 29, 1999, Ser. No. 60/156,634 filed Sep. 29, 1999, Ser. No. 60/157,280 filed Oct. 1, 1999, Ser. No. 60/157,294 filed Oct. 1, 1999, Ser. No. 60/157,281 filed Oct. 1, 1999, Ser. No. 60/157,293 filed Oct. 1, 1999, and Ser. No. 60/157,282 filed Oct. 1, 1999, the entirety of each of which is incorporated herein by reference. This patent application is related to U.S. Ser. No. 09/170,496 filed Oct. 13, 1999, and U.S. Ser. No. 09/416,760 filed Oct. 12, 1999, both being incorporated herein by reference in their entirety. This patent application is also related to U.S. Ser. No. 09/364,425 filed Jul. 30, 1999, which is incorporated herein by reference in its entirety.


FIELD OF THE INVENTION
The invention disclosed in this patent document relates to transmembrane receptors, and more particularly to endogenous, orphan, human G protein-coupled receptors (“GPCRs”).
BACKGROUND OF THE INVENTION
Although a number of receptor classes exist in humans, by far the most abundant and therapeutically relevant is represented by the G protein-coupled receptor (GPCR or GPCRs) class. It is estimated that there are some 100,000 genes within the human genome, and of these, approximately 2% or 2,000 genes, are estimated to code for GPCRs. Receptors, including GPCRs, for which the endogenous ligand has been identified are referred to as “known” receptors, while receptors for which the endogenous ligand has not been identified are referred to as “orphan” receptors. GPCRs represent an important area for the development of pharmaceutical products: from approximately 20 of the 100 known GPCRs, 60% of all prescription pharmaceuticals have been developed. This distinction is not merely semantic, particularly in the case of GPCRs. Thus, the orphan GPCRs are to the pharmaceutical industry what gold was to California in the late 19th century—an opportunity to drive growth, expansion, enhancement and development.
GPCRs share a common structural motif. All these receptors have seven sequences of between 22 to 24 hydrophobic amino acids that form seven alpha helices, each of which spans the membrane (each span is identified by number, i.e., transmembrane-1 (TM-1), transmebrane-2 (TM-2), etc.). The transmembrane helices are joined by strands of amino acids between transmembrane-2 and transmembrane-3, transmembrane-4 and transmembrane-5, and transmembrane-6 and transmembrane-7 on the exterior, or “extracellular” side, of the cell membrane (these are referred to as “extracellular” regions 1, 2 and 3 (EC-1, EC-2 and EC-3), respectively). The transmembrane helices are also joined by strands of amino acids between transmembrane-1 and transmembrane-2, transmembrane-3 and transmembrane-4, and transmembrane-5 and transmembrane-6 on the interior, or “intracellular” side, of the cell membrane (these are referred to as “intracellular” regions 1, 2 and 3 (IC-1, IC-2 and IC-3), respectively). The “carboxy” (“C”) terminus of the receptor lies in the intracellular space within the cell, and the “amino” (“N”) terminus of the receptor lies in the extracellular space outside of the cell.
Generally, when an endogenous ligand binds with the receptor (often referred to as “activation” of the receptor), there is a change in the conformation of the intracellular region that allows for coupling between the intracellular region and an intracellular “G-protein.” It has been reported that GPCRs are “promiscuous” with respect to G proteins, i.e., that a GPCR can interact with more than one G protein. See, Kenakin, T., 43 Life Sciences 1095 (1988). Although other G proteins exist, currently, Gq, Gs, Gi, and Go are G proteins that have been identified. Endogenous ligand-activated GPCR coupling with the G-protein begins a signaling cascade process (referred to as “signal transduction”). Under normal conditions, signal transduction ultimately results in cellular activation or cellular inhibition. It is thought that the IC-3 loop as well as the carboxy terminus of the receptor interact with the G protein.
Under physiological conditions, GPCRs exist in the cell membrane in equilibrium between two different conformations: an “inactive” state and an “active” state. A receptor in an inactive state is unable to link to the intracellular signaling transduction pathway to produce a biological response. Changing the receptor conformation to the active state allows linkage to the transduction pathway (via the G-protein) and produces a biological response. A receptor may be stabilized in an active state by an endogenous ligand or a compound such as a drug.
SUMMARY OF THE INVENTION
Disclosed herein are human endogenous orphan G protein-coupled receptors.



BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1A and 1B provide reference “grids” for certain dot-blots provided herein (see also, FIG. 2A and 2B, respectively).
FIGS. 2A and 2B provide reproductions of the results of certain dot-blot analyses resulting from hCHN3 and hCHN8, respectively (see also, FIGS. 1A and 1B, respectively).
FIG. 3 provides a reproduction of the results of RT-PCR analysis of hRUP3.
FIG. 4 provides a reproduction of the results of RT-PCR analysis of hRUP4.
FIG. 5 provides a reproduction of the results of RT-PCR analysis of hRUP6.
FIG. 6 is a reproduction of a photograph of the results of the tissue distribution of RUP3 using multiple tissue (human) cDNA. Based upon these tissues, the data support the position that RUP3 is expressed only in the pancreas.
FIG. 7 provides an alignment report between the putative amino acid sequence of the novel human GPCR (SEQ ID NO: 8) disclosed herein, and the reported amino acid sequence of Drosophila melanogaster, dopamine Dl-like seven-transmembrane receptor (SEQ ID NO: 75).



DETAILED DESCRIPTION
The scientific literature that has evolved around receptors has adopted a number of terms to refer to ligands having various effects on receptors. For clarity and consistency, the following definitions will be used throughout this patent document. To the extent that these definitions conflict with other definitions for these terms, the following definitions shall control:
AMINO ACID ABBREVIATIONS used herein are set out in Table 1:











TABLE 1









ALANINE
ALA
A



ARGININE
ARG
R



ASPARAGINE
ASN
N



ASPARTIC ACID
ASP
D



CYSTEINE
CYS
C



GLUTAMIC ACID
GLU
E



GLUTAMINE
GLN
Q



GLYCINE
GLY
G



HISTIDINE
HIS
H



ISOLEUCINE
ILE
I



LEUCINE
LEU
L



LYSINE
LYS
K



METHIONINE
MET
M



PHENYLALANINE
PHE
F



PROLINE
PRO
P



SERINE
SER
S



THREONINE
THR
T



TRYPTOPHAN
TRP
W



TYROSINE
TYR
Y



VALINE
VAL
V










COMPOSITION means a material comprising at least one component.
ENDOGENOUS shall mean a material that a mammal naturally produces. ENDOGENOUS in reference to, for example and not limitation, the term “receptor,” shall mean that which is naturally produced by a mammal (for example, and not limitation, a human) or a virus. By contrast, the term NON-ENDOGENOUS in this context shall mean that which is not naturally produced by a mammal (for example, and not limitation, a human) or a virus.
HOST CELL shall mean a cell capable of having a Plasmid and/or Vector incorporated therein. In the case of a prokaryotic Host Cell, a Plasmid is typically replicated as a autonomous molecule as the Host Cell replicates (generally, the Plasmid is thereafter isolated for introduction into a eukaryotic Host Cell); in the case of a eukaryotic Host Cell, a Plasmid is integrated into the cellular DNA of the Host Cell such that when the eukaryotic Host Cell replicates, the Plasmid replicates. Preferably, for the purposes of the invention disclosed herein, the Host Cell is eukaryotic, more preferably, mammalian, and most preferably selected from the group consisting of 293, 293T and COS-7 cells.
LIGAND shall mean an endogenous, naturally occurring molecule specific for an endogenous, naturally occurring receptor.
MUTANT or MUTATION in reference to an endogenous receptor's nucleic acid and/or amino acid sequence shall mean a specified change or changes to such endogenous sequences such that a mutated form of an endogenous, non-constitutively activated receptor evidences constitutive activation of the receptor. In terms of equivalents to specific sequences, a subsequent mutated form of a human receptor is considered to be equivalent to a first mutation of the human receptor if (a) the level of constitutive activation of the subsequent mutated form of the receptor is substantially the same as that evidenced by the first mutation of the receptor; and (b) the percent sequence (amino acid and/or nucleic acid) homology between the subsequent mutated form of the receptor and the first mutation of the receptor is at least about 80%, more preferably at least about 90% and most preferably at least 95%. Ideally, and owing to the fact that the most preferred mutation disclosed herein for achieving constitutive activation includes a single amino acid and/or codon change between the endogenous and the non-endogenous forms of the GPCR, the percent sequence homology should be at least 98%.
NON-ORPHAN RECEPTOR shall mean an endogenous naturally occurring molecule specific for an endogenous naturally occurring ligand wherein the binding of a ligand to a receptor activates an intracellular signaling pathway.
ORPHAN RECEPTOR shall mean an endogenous receptor for which the endogenous ligand specific for that receptor has not been identified or is not known.
PLASMID shall mean the combination of a Vector and cDNA. Generally, a Plasmid is introduced into a Host Cell for the purposes of replication and/or expression of the cDNA as a protein.
VECTOR sin reference to cDNA shall mean a circular DNA capable of incorporating at least one cDNA and capable of incorporation into a Host Cell.
The order of the following sections is set forth for presentational efficiency and is not intended, nor should be construed, as a limitation on the disclosure or the claims to follow.
A. Identification of Human GPCRs
The efforts of the Human Genome project have led to the identification of a plethora of information regarding nucleic acid sequences located within the human genome; it has been the case in this endeavor that genetic sequence information has been made available without an understanding or recognition as to whether or not any particular genomic sequence does or may contain open-reading frame information that translate human proteins. Several methods of identifying nucleic acid sequences within the human genome are within the purview of those having ordinary skill in the art. For example, and not limitation, a variety of GPCRs, disclosed herein, were discovered by reviewing the GenBankTM database, while other GPCRs were discovered by utilizing a nucleic acid sequence of a GPCR, previously sequenced, to conduct a BLASTTM search of the EST database. Table A, below, lists the disclosed endogenous orphan GPCRs along with a GPCR's respective homologous GPCR:











TABLE A





Disclosed


Per Cent
Reference To


Human
Accession
Open Reading
Homology
Homologous


Orphan
Number
Frame
To Designated
GPCR


GPCRs
Identified
(Base Pairs)
GPCR
(Accession No.)




















hARE-3
AL033379
1,260
bp
52.3% LPA-R
U92642


hARE-4
AC006087
1,119
bp
36% P2Y5
AF000546


hARE-5
AC006255
1,104
bp
32% Oryzias
D43633






latipes


hGPR27
AA775870
1,128
bp


hARE-1
AI090920
999
bp
43% KIAA0001
D13626


hARE-2
AA359504
1,122
bp
53% GPR27


hPPR1
H67224
1,053
bp
39% EBI1
L31581


hG2A
AA754702
1,113
bp
31% GPR4
L36148


hRUP3
AL035423
1,005
bp
30%
2133653






Drosophila






melanogaster


hRUP4
AI307658
1,296
bp
32% pNPGPR
NP_004876






28% and 29%
AAC41276






Zebra fish
and






Ya and Yb,
AAB94616






respectively


hRUP5
AC005849
1,413
bp
25% DEZ
Q99788






23% FMLPR
P21462


hRUP6
AC005871
1,245
bp
48% GPR66
NP_006047


hRUP7
AC007922
1,173
bp
43% H3R
AF140538


hCHN3
EST 36581
1,113
bp
53% GPR27


hCHN4
AA804531
1,077
bp
32% thrombin
4503637


hCHN6
EST 2134670
1,503
bp
36% edg-1
NP_001391


hCHN8
EST 764455
1,029
bp
47%
D13626






KIAA0001


hCHN9
EST 1541536
1,077
bp
41% LTB4R
NM_000752


hCHN10
EST 1365839
1,055
bp
35% P2Y
NM_002563









Receptor homology is useful in terms of gaining an appreciation of a role of the disclosed receptors within the human body. Additionally, such homology can provide insight as to possible endogenous ligand(s) that may be natural activators for the disclosed orphan GPCRs.
B. Receptor Screening
Techniques have become more readily available over the past few years for endogenous-ligand identification (this, primarily, for the purpose of providing a means of conducting receptor-binding assays that require a receptor's endogenous ligand) because the traditional study of receptors has always proceeded from the a priori assumption (historically based) that the endogenous ligand must first be identified before discovery could proceed to find antagonists and other molecules that could affect the receptor. Even in cases where an antagonist might have been known first, the search immediately extended to looking for the endogenous ligand. This mode of thinking has persisted in receptor research even after the discovery of constitutively activated receptors. What has not been heretofore recognized is that it is the active state of the receptor that is most useful for discovering agonists, partial agonists, and inverse agonists of the receptor. For those diseases which result from an overly active receptor or an under-active receptor, what is desired in a therapeutic drug is a compound which acts to diminish the active state of a receptor or enhance the activity of the receptor, respectively, not necessarily a drug which is an antagonist to the endogenous ligand. This is because a compound that reduces or enhances the activity of the active receptor state need not bind at the same site as the endogenous ligand. Thus, as taught by a method of this invention, any search for therapeutic compounds should start by screening compounds against the ligand-independent active state.
As is known in the art, GPCRs can be “active” in their endogenous state even without the binding of the receptor's endogenous ligand thereto. Such naturally-active receptors can be screened for the direct identification (i.e., without the need for the receptor's endogenous ligand) of, in particular, inverse agonists. Alternatively, the receptor can be “activated” via, e.g., mutation of the receptor to establish a non-endogenous version of the receptor that is active in the absence of the receptor's endogenous ligand.
Screening candidate compounds against an endogenous or non-endogenous, constitutively activated version of the human orphan GPCRs disclosed herein can provide for the direct identification of candidate compounds which act at this cell surface receptor, without requiring use of the receptor's endogenous ligand. By determining areas within the body where the endogenous version of human GPCRs disclosed herein is expressed and/or over-expressed, it is possible to determine related disease/disorder states which are associated with the expression and/or over-expression of the receptor; such an approach is disclosed in this patent document.
With respect to creation of a mutation that may evidence constitutive activation of human orphan GPCRs disclosed herein is based upon the distance from the proline residue at which is presumed to be located within TM6 of the GPCR typically nears the TM6/IC3 interface (such proline residue appears to be quite conserved). By mutating the amino acid residue located 16 amino acid residues from this residue (presumably located in the IC3 region of the receptor) to, most preferably, a lysine residue, such activation may be obtained. Other amino acid residues may be useful in the mutation at this position to achieve this objective.
C. Disease/Disorder Identification and/or Selection
Preferably, the DNA sequence of the human orphan GPCR can be used to make a probe for (a) dot-blot analysis against tissue-mRNA, and/or (b) RT-PCR identification of the expression of the receptor in tissue samples. The presence of a receptor in a tissue source, or a diseased tissue, or the presence of the receptor at elevated concentrations in diseased tissue compared to a normal tissue, can be preferably utilized to identify a correlation with a treatment regimen, including but not limited to, a disease associated with that disease. Receptors can equally well be localized to regions of organs by this technique. Based on the known functions of the specific tissues to which the receptor is localized, the putative functional role of the receptor can be deduced.
As the data below indicate, RUP3 is expressed within the human pancreas, suggesting that RUP3 may play a role in insulin regulation and/or glucagon regulation. Accordingly, candidate compounds identified using a constitutively activated form of RUP3 may be useful for understanding the role of RUP3 in diabetes and/or as therapeutics for diabetes.
D. Screening of Candidate Compounds
1. Generic GPCR Screening Assay Techniques
When a G protein receptor becomes constitutively active (i.e., active in the absence of endogenous ligand binding thereto), it binds to a G protein (e.g., Gq, Gs, Gi, Go) and stimulates the binding of GTP to the G protein. The G protein then acts as a GTPase and slowly hydrolyzes the GTP to GDP, whereby the receptor, under normal conditions, becomes deactivated. However, constitutively activated receptors continue to exchange GDP to GTP. A non-hydrolyzable analog of GTP, [35S]GTPγS, can be used to monitor enhanced binding to membranes which express constitutively activated receptors. It is reported that [35S]GTPγS can be used to monitor G protein coupling to membranes in the absence and presence of ligand. An example of this monitoring, among other examples well-known and available to those in the art, was reported by Traynor and Nahorski in 1995. The preferred use of this assay system is for initial screening of candidate compounds because the system is generically applicable to all G protein-coupled receptors regardless of the particular G protein that interacts with the intracellular domain of the receptor.
2. Specific GPCR Screening Assay Techniques
Once candidate compounds are identified using the “generic” G protein-coupled receptor assay (i.e., an assay to select compounds that are agonists, partial agonists, or inverse agonists), further screening to confirm that the compounds have interacted at the receptor site is preferred. For example, a compound identified by the “generic” assay may not bind to the receptor, but may instead merely “uncouple” the G protein from the intracellular domain.
a. Gs and Gi.
Gs stimulates the enzyme adenylyl cyclase. Gi (and Go), on the other hand, inhibit this enzyme. Adenylyl cyclase catalyzes the conversion of ATP to cAMP; thus, constitutively activated GPCRs that couple the Gs protein are associated with increased cellular levels of cAMP. On the other hand, constitutively activated GPCRs that couple the Gi (or Go) protein are associated with decreased cellular levels of cAMP. See, generally, “Indirect Mechanisms of Synaptic Transmission,” Chpt. 8, From Neuron To Brain (3rd Ed.) Nichols, J. G. et al eds. Sinauer Associates, Inc. (1992). Thus, assays that detect cAMP can be utilized to determine if a candidate compound is, e.g., an inverse agonist to the receptor (i.e., such a compound would decrease the levels of cAMP). A variety of approaches known in the art for measuring cAMP can be utilized; a most preferred approach relies upon the use of anti-cAMP antibodies in an ELISA-based format. Another type of assay that can be utilized is a whole cell second messenger reporter system assay. Promoters on genes drive the expression of the proteins that a particular gene encodes. Cyclic AMP drives gene expression by promoting the binding of a cAMP-responsive DNA binding protein or transcription factor (CREB) which then binds to the promoter at specific sites called cAMP response elements and drives the expression of the gene. Reporter systems can be constructed which have a promoter containing multiple cAMP response elements before the reporter gene, e.g., β-galactosidase or luciferase. Thus, a constitutively activated Gs-linked receptor causes the accumulation of cAMP that then activates the gene and expression of the reporter protein. The reporter protein such as β-galactosidase or luciferase can then be detected using standard biochemical assays (Chen et al. 1995).
b. Go and Gq.
Gq and Go are associated with activation of the enzyme phospholipase C, which in turn hydrolyzes the phospholipid PIP2, releasing two intracellular messengers: diacycloglycerol (DAG) and inistol 1,4,5-triphoisphate (IP3). Increased accumulation of IP3 is associated with activation of Gq- and Go-associated receptors. See, generally, “Indirect Mechanisms of Synaptic Transmission,” Chpt. 8, From Neuron To Brain (3rd Ed.) Nichols, J. G. et al eds. Sinauer Associates, Inc. (1992). Assays that detect IP3 accumulation can be utilized to determine if a candidate compound is, e.g., an inverse agonist to a Gq- or Go-associated receptor (i.e., such a compound would decrease the levels of IP3). Gq-associated receptors can also been examined using an AP1 reporter assay in that Gq-dependent phospholipase C causes activation of genes containing AP1 elements; thus, activated Gq-associated receptors will evidence an increase in the expression of such genes, whereby inverse agonists thereto will evidence a decrease in such expression, and agonists will evidence an increase in such expression. Commercially available assays for such detection are available.
3. GPCR Fusion Protein
The use of an endogenous, constitutively activated orphan GPCR, or a non-endogenous, constitutively activated orphan GPCR, for screening of candidate compounds for the direct identification of inverse agonists, agonists and partial agonists provides a unique challenge in that, by definition, the receptor is active even in the absence of an endogenous ligand bound thereto. Thus, it is often useful that an approach be utilized that can enhance the signal obtained by the activated receptor. A preferred approach is the use of a GPCR Fusion Protein.
Generally, once it is determined that a GPCR is or has been constitutively activated, using the assay techniques set forth above (as well as others), it is possible to determine the predominant G protein that couples with the endogenous GPCR. Coupling of the G protein to the GPCR provides a signaling pathway that can be assessed. Because it is most preferred that screening take place by use of a mammalian expression system, such a system will be expected to have endogenous G protein therein. Thus, by definition, in such a system, the constitutively activated orphan GPCR will continuously signal. In this regard, it is preferred that this signal be enhanced such that in the presence of, e.g., an inverse agonist to the receptor, it is more likely that it will be able to more readily differentiate, particularly in the context of screening, between the receptor when it is contacted with the inverse agonist.
The GPCR Fusion Protein is intended to enhance the efficacy of G protein coupling with the GPCR. The GPCR Fusion Protein is preferred for screening with a non-endogenous, constitutively activated GPCR because such an approach increases the signal that is most preferably utilized in such screening techniques, although the GPCR Fusion Protein can also be (and preferably is) used with an endogenous, constitutively activated GPCR. This is important in facilitating a significant “signal to noise” ratio; such a significant ratio is import preferred for the screening of candidate compounds as disclosed herein.
The construction of a construct useful for expression of a GPCR Fusion Protein is within the purview of those having ordinary skill in the art. Commercially available expression vectors and systems offer a variety of approaches that can fit the particular needs of an investigator. The criteria of importance for such a GPCR Fusion Protein construct is that the GPCR sequence and the G protein sequence both be in-frame (preferably, the sequence for the GPCR is upstream of the G protein sequence) and that the “stop” codon of the GPCR must be deleted or replaced such that upon expression of the GPCR, the G protein can also be expressed. The GPCR can be linked directly to the G protein, or there can be spacer residues between the two (preferably, no more than about 12, although this number can be readily ascertained by one of ordinary skill in the art). We have a preference (based upon convenience) of use of a spacer in that some restriction sites that are not used will, effectively, upon expression, become a spacer. Most preferably, the G protein that couples to the GPCR will have been identified prior to the creation of the GPCR Fusion Protein construct. Because there are only a few G proteins that have been identified, it is preferred that a construct comprising the sequence of the G protein (i.e., a universal G protein construct) be available for insertion of an endogenous GPCR sequence therein; this provides for efficiency in the context of large-scale screening of a variety of different endogenous GPCRs having different sequences.
E. Other Utility
Although a preferred use of the human orphan GPCRs disclosed herein may be for the direct identification of candidate compounds as inverse agonists, agonists or partial agonists (preferably for use as pharmaceutical agents), these versions of human GPCRs can also be utilized in research settings. For example, in vitro and in vivo systems incorporating GPCRs can be utilized to further elucidate and understand the roles these receptors play in the human condition, both normal and diseased, as well as understanding the role of constitutive activation as it applies to understanding the signaling cascade. The value in human orphan GPCRs is that its utility as a research tool is enhanced in that by determining the location(s) of such receptors within the body, the GPCRs can be used to understand the role of these receptors in the human body before the endogenous ligand therefor is identified. Other uses of the disclosed receptors will become apparent to those in the art based upon, inter alia, a review of this patent document.
Although a preferred use of the non-endogenous versions of the human RUP3 disclosed herein may be for the direct identification of candidate compounds as inverse agonists, agonists or partial agonists (preferably for use as pharmaceutical agents), this version of human RUP3 can also be utilized in research settings. For example, in vitro and in vivo systems incorporating RUP3 can be utilized to further elucidate the roles RUP3 plays in the human condition, particularly with respect to the human pancreas, both normal and diseased (and in particular, diseases involving regulation of insulin or glucagon, e.g., diabetes), as well as understanding the role of constitutive activation as it applies to understanding the signaling cascade. A value in non-endogenous human RUP3 is that its utility as a research tool is enhanced in that, because of its unique features, non-endogenous RUP3 can be used to understand the role of RUP3 in the human body before the endogenous ligand therefor is identified. Other uses of the disclosed receptors will become apparent to those in the art based upon, inter alia, a review of the patent document.
EXAMPLES
The following examples are presented for purposes of elucidation, and not limitation, of the present invention. While specific nucleic acid and amino acid sequences are disclosed herein, those of ordinary skill in the art are credited with the ability to make minor modifications to these sequences while achieving the same or substantially similar results reported below. Unless otherwise indicated below, all nucleic acid sequences for the disclosed endogenous orphan human GPCRs have been sequenced and verified. For purposes of equivalent receptors, those of ordinary skill in the art will readily appreciate that conservative substitutions can be made to the disclosed sequences to obtain a functionally equivalent receptor.
Example 1
Endogenous Human GPCRs
1. Identification of Human GPCRs
Several of the disclosed endogenous human GPCRs were identified based upon a review of the GenBank database information. While searching the database, the following cDNA clones were identified as evidenced below.















Disclosed

Complete

Nucleic
Amino


Human

DNA
Open Reading
Acid
Acid


Orphan
Accession
Sequence
Frame
SEQ.
SEQ.


GPCRs
Number
(Base Pairs)
(Base Pairs)
ID. NO.
ID. NO.




















hARE-3
AL033379
111,389 bp
1,260 bp
1
2


hARE-4
AC006087
226,925 bp
1,119 bp
3
4


hARE-5
AC006255
127,605 bp
1,104 bp
5
6


hRUP3
AL035423
140,094 bp
1,005 bp
7
8


hRUP5
AC005849
169,144 bp
1,413 bp
9
10


hRUP6
AC005871
218,807 bp
1,245 bp
11
12


hRUP7
AC007922
158,858 bp
1,173 bp
13
14









Other disclosed endogenous human GPCRs were identified by conducting a BLAST search of EST database (dbest) using the following EST clones as query sequences. The following EST clones identified were then used as a probe to screen a human genomic library.















Disclosed







Human


Open Reading


Orphan
Query
EST Clone/Accession
Frame
Nucleic Acid
Amino Acid


GPCRs
(Sequence)
No. Identified
(Base Pairs)
SEQ. ID. NO.
SEQ. ID. NO.





















hGPCR27
Mouse
AA775870
1,125
bp
15
16



GPCR27


hARE-1
TDAG
1689643
999
bp
17
18




AI090920


hARE-2
GPCR27
68530
1,122
bp
19
20




AA359504


hPPR1
Bovine
238667
1,053
bp
21
22



PPR1
H67224


hG2A
Mouse
See Example 2(a),
1,113
bp
23
24



1179426
below


hCHN3
N.A.
EST 36581
1,113
bp
25
26




(full length)


hCHN4
TDAG
1184934
1,077
bp
27
28




AA804531


hCHN6
N.A.
EST 2134670
1,503
bp
29
30




(full length)


hCHN8
KIAA0001
EST 764455
1,029
bp
31
32


hCHN9
1365839
EST 1541536
1,077
bp
33
34


hCHN10
Mouse EST
Human 1365839
1,005
bp
35
36



1365839


hRUP4
N.A.
AI307658
1,296
bp
37
38





N.A. = “not applicable”.






2. Full Length Cloning
a. hG2A (Seq. Id. Nos. 23 & 24)
Mouse EST clone 1179426 was used to obtain a human genomic clone containing all but three amino acid hG2A coding sequences. The 5′end of this coding sequence was obtained by using 5′RACETM, and the template for PCR was Clontech's Human Spleen Marathon-readyTM cDNA. The disclosed human G2A was amplified by PCR using the G2A cDNA specific primers for the first and second round PCR as shown in SEQ.ID.NO.: 39 and SEQ.ID.NO.:40 as follows:









5′-CTGTGTACAGCAGTTCGCAGAGTG-3′
(SEQ.ID.NO.: 39;




1st round PCR)


 


5′-GAGTGCCAGGCAGAGCAGGTAGAC-3′
(SEQ.ID.NO.: 40;



second round PCR).






PCR was performed using AdvantageTM GC Polymerase Kit (Clontech; manufacturing instructions will be followed), at 94° C. for 30 sec followed by 5 cycles of 94° C. for 5 sec and 72° C. for 4 min; and 30 cycles of 94° for 5 sec and 70° for 4 min. An approximate 1.3 Kb PCR fragment was purified from agarose gel, digested with Hind III and Xba I and cloned into the expression vector pRC/CMV2 (Invitrogen). The cloned-insert was sequenced using the T7 SequenaseTM kit (USB Amersham; manufacturer instructions will be followed) and the sequence was compared with the presented sequence. Expression of the human G2A will be detected by probing an RNA dot blot (Clontech; manufacturer instructions will be followed) with the P32-labeled fragment.

b. hCHN9 (Seq. Id. Nos. 33 & 34)
Sequencing of the EST clone 1541536 indicated that hCHN9 is a partial cDNA clone having only an initiation codon; i.e., the termination codon was missing. When hCHN9 was used to “blast” against the data base (nr), the 3′ sequence of hCHN9 was 100% homologous to the 5′ untranslated region of the leukotriene B4 receptor cDNA, which contained a termination codon in the frame with hCHN9 coding sequence. To determine whether the 5′ untranslated region of LTB4R cDNA was the 3′ sequence of hCHN9, PCR was performed using primers based upon the 5′ sequence flanking the initiation codon found in hCHN9 and the 3′ sequence around the termination codon found in the LTB4R 5′ untranslated region. The 5′ primer sequence utilized was as follows:










(SEQ.ID.NO.: 41; sense)











5′-CCCGAATTCCTGCTTGCTCCCAGCTTGGCCC-3′




and



 










(SEQ.ID.NO.: 42; antisense).











5′-TGTGGATCCTGCTGTCAAAGGTCCCATTCCGG-3′







PCR was performed using thymus cDNA as a template and rTth polymerase (Perkin Elmer) with the buffer system provided by the manufacturer, 0.25 uM of each primer, and 0.2 mM of each 4 nucleotides. The cycle condition was 30 cycles of 94° C. for 1 min, 65° C. for 1 min and 72° C. for 1 min and 10 sec. A 1.1 kb fragment consistent with the predicted size was obtained from PCR. This PCR fragment was subcloned into pCMV (see below) and sequenced (see, SEQ.ID.NO.: 33).

c. HRUP 4 (Seq. Id. Nos. 37 & 38)
The full length hRUP4 was cloned by RT-PCR with human brain cDNA (Clontech) as templates:









5′-TCACAATGGTAGGTGTGGTC-3′
(SEQ.ID.NO.: 43;




sense)


and


 


5′-TGCATAGACAATGGGATTACAG-3′
(SEQ.ID.NO.: 44;



antisense).






PCR was performed using TaqPlusTM PrecisionTM polymerase (Stratagene; manufacturing instructions will be followed) by the following cycles: 94° C. for 2 min; 94° C. 30 sec; 55° C. for 30 sec, 72° C. for 45 sec, and 72° C. for 10 min. Cycles 2 through 4 were repeated 30 times.

The PCR products were separated on a 1% agarose gel and a 500 bp PCR fragment was isolated and cloned into the pCRII-TOPO vector (Invitrogen) and sequenced using the T7 DNA SequenaseTM kit (Amsham) and the SP6/T7 primers (Stratagene). Sequence analysis revealed that the PCR fragment was indeed an alternatively spliced form of AI307658 having a continuous open reading frame with similarity to other GPCRs. The completed sequence of this PCR fragment was as follows:









5′-TCACAATGCTAGGTGTGGTCTGGCTGGTGGCAGTCATCGTAGGATGACCCATGTGGCAC
(SEQ.ID.NO.: 45)



 


GTGCAACAACTTGAGATCAAATATGACTTCCTATATGAAAAGGAACACATCTGCTGCTTA


 


GAAGAGTGGACCAGCCCTGTGCACCAGAAGATCTACACCACCTTCATCCTTGTCATCCTCT


 


TCCTCCTGCCTCTTATGGTGATGCTTATTCTGTACGTAAAATTGGTTATGAACTTTGGATAA


 


AGAAAAGAGTTGGGGATGGTTCAGTGCTTCGAACTATTCATGGAAAAGAAATGTCCAAAA


 


TAGCCAGGAAGAAGAAACGAGCTGTCATTATGATGGTGACAGTGGTGGCTCTCTTTGCTG


 


TGTGCTGGGCACCATTCCATGTTGTCCATATGATGATTGAATACAGTAATTTTGAAAAGGA


 


ATATGATGATGTCACAATCAAGATGATTTTTGCTATCGTGCAAATTATTGGATTTTCCAAC


 


TCCATCTGTAATCCCATTGTCTATGCA-3′






Based on the above sequence, two sense oligonucleotide primer sets:









5′-CTGCTTAGAAGAGTGGACCAG-3′
(SEQ.ID.NO.: 46;




oligo 1),


 


5′-CTGTGCACCAGAAGATCTACAC-3′
(SEQ.IDNO.: 47;



oligo 2)






and two antisense oligonucleotide primer sets:










5′-CAAGGATGAAGGTGGTGTAGA-3′
(SEQ.ID.NO.: 48;




oligo 3)


 


5′-GTGTAGATCTTCTGGTGCACAGG-3′
(SEQ.ID.NO.: 49;



oligo 4)






were used for 3+- and 5′-race PCR with a human brain Marathon-ReadyTM cDNA (Clontech, Cat# 7400-1) as template, according to manufacture's instructions. DNA fragments generated by the RACE PCR were cloned into the pCRII-TOPOTM vector (Invitrogen) and sequenced using the SP6/T7 primers (Stratagene) and some internal primers. The 3′ RACE product contained a poly(A) tail and a completed open reading frame ending at a TAA stop codon. The 5′ RACE product contained an incomplete 5′ end; i.e., the ATG initiation codon was not present.

Based on the new 5′ sequence, oligo 3 and the following primer:











5′-GCAATGCAGGTCATAGTGAGC-3′
(SEQ.ID.NO.: 50;





oligo 5)






were used for the second round of 5′ RACE PCR and the PCR products were analyzed as above. A third round of 5′ RACE PCR was carried out utilizing antisense primers:










5′-TGGAGCATGGTGACGGGAATGCAGAAG-3′
(SEQ.ID.NO.: 51;




oligo 6)


and


 


5′-GTGATGAGCAGGTCACTGAGCGCCAAG-3′
(SEQ.ID.NO.: 52;



oligo 7).






The sequence of the 5′ RACE PCR products revealed the presence of the initiation codon ATG, and further round of 5′ RACE PCR did not generate any more 5′ sequence. The completed 5′ sequence was confirmed by RT-PCR using sense primer










5′-GCAATGCAGGCGGTTAACATTAC-3′
(SEQ.ID.NO.: 53;




oligo 8)






and oligo 4 as primers and sequence analysis of the 650 bp PCR product generated from human brain and heart cDNA templates (Clontech, Cat# 7404-1). The completed 3′ sequence was confirmed by RT-PCR using oligo 2 and the following antisense primer:










5′-TTGGGTTACAATCTGAAGGGCA-3′
(SEQ.ID.NO.: 54;




oligo 9)






and sequence analysis of the 670 bp PCR product generated from human brain and heart cDNA templates. (Clontech, Cat# 7404-1).

d. hRUP5 (Seq. Id. Nos. 9 & 10)
The full length hRUP5 was cloned by RT-PCR using a sense primer upstream from ATG, the initiation codon (SEQ.ID.NO.: 55), and an antisense primer containing TCA as the stop codon (SEQ.ID.NO.: 56), which had the following sequences:









5′-ACTCGGTGTCCAGCAGGACTCTG-3′
(SEQ.ID.NO.: 55)



 


5′-TGCGTGTTCCTGGACCCTCACGTG-3′
(SEQ.ID.NO.: 56)






and human peripheral leukocyte cDNA (Clontech) as a template. Advantage cDNA polymerase (Clontech) was used for the amplification in a 50 ul reaction by the following cycle with step 2 through step 4 repeated 30 times: 94° C. for 30 sec; 94° for 15 sec; 69° C. for 40 sec; 72° C. for 3 min; and 72° C. fro 6 min. A 1.4 kb PCR fragment was isolated and cloned with the pCRII-TOPOTM vector (Invitrogen) and completely sequenced using the T7 DNA SequenaseTM kit (Amsham). See, SEQ.ID.NO.: 9.

e. hRUP6 (Seq. Id. Nos. 11 & 12)
The full length hRUP6 was cloned by RT-PCR using primers:










(SEQ.ID.NO.: 57)











5′-CAGGCCTTGGATTTTAATGTCAGGGATGG-3′




and



 










(SEQ.ID.NO.: 58)











5′-GGAGAGTCAGCTCTGAAAGAATTCAGG-3′;







and human thymus Marathon-ReadyTM cDNA (Clontech) as a template. Advantage cDNA polymerase (Clontech, according to manufacturer's instructions) was used for the amplification in a 50 ul reaction by the following cycle: 94° C. for 30sec; 94° C. for 5 sec; 66° C. for 40sec; 72° C. for 2.5 sec and 72° C. for 7 min. Cycles 2 through 4 were repeated 30 times. A 1.3 Kb PCR fragment was isolated and cloned into the pCRII-TOPOTM vector (Invitrogen) and completely sequenced (see, SEQ.ID.NO.: 11) using the ABI Big Dye TerminatorTM kit (P.E. Biosystem).

f. hRUP7 (Seq. Id. Nos. 13 & 14)
The full length RUP7 was cloned by RT-PCR using primers:









5′-TGATGTGATGCCAGATACTAATAGCAC-3′
(SEQ.ID.NO.: 59;




sense)


and


 


5′-CCTGATTCATTTAGGTGAGATTGAGAC-3′
(SEQ.ID.NO.: 60;



antisense)






and human peripheral leukocyte cDNA (Clontech) as a template. AdvantageTM cDNA polymerase (Clontech) was used for the amplification in a 50 ul reaction by the following cycle with step 2 to step 4 repeated 30 times: 94° C. for 2 minutes; 94° C. for 15 seconds; 60° C. for 20 seconds; 72° C. for 2 minutes; 72° C. for 10 minutes. A 1.25 Kb PCR fragment was isolated and cloned into the pCRII-TOPOTM vector (Invitrogen) and completely sequenced using the ABI Big Dye TerminatorTM kit (P.E. Biosystem). See, SEQ.ID.NO.: 13.

g. hARE-5 (Seq. Id. Nos. 5 & 6)
The full length hARE-5 was cloned by PCR using the hARE5 specific primers 5′-CAGCGCAGGGTGAAGCCTGAGAGC-3′ SEQ.ID.NO.: 69 (sense, 5′ of initiation codon ATG) and 5′-GGCACCTGCTGTGACCTGTGCAGG-3′ SEQ.ID.NO.:70 (antisense, 3′ of stop codon TGA) and human genomic DNA as template. TaqPlus PrecisionTM DNA polymerase (Stratagene) was used for the amplification by the following cycle with step 2 to step 4 repeated 35 times: 96° C., 2 minutes; 96° C., 20 seconds; 58° C., 30 seconds; 72° C., 2 minutes; and 72° C., 10 minutes
A 1.1 Kb PCR fragment of predicated size was isolated and cloned into the pCRII-TOPOTM vector (Invitrogen) and completely sequenced (SEQ.ID.NO.:5) using the T7 DNA SequenaseTM kit (Amsham).
h. hARE-4 (Seq. Id. Nos.: 3 & 4)
The full length hARE-4 was cloned by PCR using the hARE-4 specific primers 5′-CTGGTGTGCTCCATGGCATCCC-3′ SEQ.ID.NO.:67 (sense, 5′ of initiation codon ATG) and 5′-GTAAGCCTCCCAGAACGAGAGG-3′ SEQ.ID.NO.: 68 (antisense, 3′ of stop codon TGA) and human genomic DNA as template. Taq DNA polymerase (Stratagene) and 5% DMSO was used for the amplification by the following cycle with step 2 to step 3 repeated 35 times: 94° C., 3 minutes; 94° C., 30 seconds; 59° C., 2 minutes; 72° C., 10 minutes
A 1.12 Kb PCR fragment of predicated size was isolated and cloned into the pCRII-TOPOTM vector (Invitrogen) and completely sequenced (SEQ.ID.NO.:3) using the T7 DNA SequenaseTM kit (Amsham).
i. hARE-3 (Seq.Id.Nos.: 1 & 2)
The full length hARE-3 was cloned by PCR using the hARE-3 specific primers 5′-gatcaagcttCCATCCTACTGAAACCATGGTC-3′ SEQ.ID.NO.:65 (sense, lower case nucleotides represent HindIII overhang, ATG as initiation codon) and 5′-gatcagatctCAGTTCCAATATTCACACCACCGTC-3′ SEQ.ID.NO.:66 (antisense, lower case nucleotides represent XbaI overhang, TCA as stop codon) and human genomic DNA as template. TaqPlus PrecisionTM DNA polymerase (Stratagene) was used for the amplification by the following cycle with step 2 to step 4 repeated 35 times: 94° C., 3 minutes; 94° C., 1 minute; 55° C., 1 minute; 72° C., 2 minutes; 72° C., 10 minutes.
A 1.3 Kb PCR fragment of predicated size was isolated and digested with HindIII and XbaI, cloned into the pRC/CMV2 vector (Invitrogen) at the HindIII and XbaI sites and completely sequenced (SEQ.ID.NO.:1) using the T7 DNA SequenaseTM kit (Amsham).
j. hRUP3 (Seq. Id. Nos.:7 & 8)
The full length hRUP3 was cloned by PCR using the hRUP3 specific primers 5′-GTCCTGCCACTTCGAGACATGG-3′ SEQ.ID.NO.:71 (sense, ATG as initiation codon) and 5′-GAAACTTCTCTGCCCTTACCGTC-3′ SEQ.ID.NO.:72 (antisense, 3′ of stop codon TAA) and human genomic DNA as template. TaqPlus PrecisionTM DNA polymerase (Stratagene) was used for the amplification by the following cycle with step 2 to step 4 repeated 35 times: 94° C., 3 minutes; 94° C., 1 minute; 58° C., 1 minute; 72° C., 2 minutes; 72° C., 10 minutes
A 1.0 Kb PCR fragment of predicated size was isolated and cloned into the pCRII-TOPOTM vector (Invitrogen) and completely sequenced (SEQ.ID.NO.: 7)using the T7 DNA sequenase kit (Amsham).
Example 2
Receptor Expression
Although a variety of cells are available to the art for the expression of proteins, it is most preferred that mammalian cells be utilized. The primary reason for this is predicated upon practicalities, i.e., utilization of, e.g., yeast cells for the expression of a GPCR, while possible, introduces into the protocol a non-mammalian cell which may not (indeed, in the case of yeast, does not) include the receptor-coupling, genetic-mechanism and secretary pathways that have evolved for mammalian systems—thus, results obtained in non-mammalian cells, while of potential use, are not as preferred as that obtained from mammalian cells. Of the mammalian cells, COS-7, 293 and 293T cells are particularly preferred, although the specific mammalian cell utilized can be predicated upon the particular needs of the artisan. The general procedure for expression of the disclosed GPCRs is as follows.
On day one, 1X107 293T cells per 150 mm plate were plated out. On day two, two reaction tubes will be prepared (the proportions to follow for each tube are per plate): tube A will be prepared by mixing 20 μg DNA (e.g., pCMV vector; pCMV vector with receptor cDNA, etc.) in 1.2 ml serum free DMEM (Irvine Scientific, Irvine, Calif.); tube B will be prepared by mixing 120 μl lipofectamine (Gibco BRL) in 1.2 ml serum free DMEM. Tubes A and B are admixed by inversions (several times), followed by incubation at room temperature for 30-45min. The admixture can be referred to as the “transfection mixture”. Plated 293T cells are washed with 1XPBS, followed by addition of 10 ml serum free DMEM. 2.4 ml of the transfection mixture will then be added to the cells, followed by incubation for 4 hrs at 37° C./5% CO2. The transfection mixture was then be removed by aspiration, followed by the addition of 25 ml of DMEM/10% Fetal Bovine Serum. Cells will then be incubated at 37° C./5% CO2. After 72 hr incubation, cells can then be harvested and utilized for analysis.
Example 3
Tissue Distribution of the Disclosed Human GPCRs
Several approaches can be used for determination of the tissue distribution of the GPCRs disclosed herein.
1. Dot-Blot Analysis
Using a commercially available human-tissue dot-blot format, endogenous orphan GPCRs were probed for a determination of the areas where such receptors are localized. cDNA fragments from the GPCRs of Example 1 (radiolabelled) were (or can be) used as the probe: radiolabeled probe was (or can be) generated using the complete receptor cDNA (excised from the vector) using a Prime-It IITM Random Primer Labeling Kit (Stratagene, #300385), according to manufacturer's instructions. A human RNA Master BlotTM (Clontech, #7770-1) was hybridized with the endogenous human GPCR radiolabeled probe and washed under stringent conditions according manufacturer's instructions. The blot was exposed to Kodak BioMaXTM Autoradiography film overnight at −80° C. Results are summarized for several receptors in Table B and C (see FIGS. 1A and 1B for a grid identifying the various tissues and their locations, respectively). Exemplary dot-blots are provided in FIG. 2A and 2B for results derived using hCHN3 and hCHN8, respectively.








TABLE B





ORPHAN
Tissue Distribution


GPCR
(highest levels, relative to other tissues in the dot-blot)







hGPCR27
Fetal brain, Putamen, Pituitary gland, Caudate nucleus


hARE-1
Spleen, Peripheral leukocytes, Fetal spleen


hPPR1
Pituitary gland, Heart, salivary gland, Small intestine, Testis


hRUP3
Pancreas


hCHN3
Fetal brain, Putamen, Occipital cortex


hCHN9
Pancreas, Small intestine, Liver


hCHN10
Kidney, Thryoid

















TABLE C






Tissue Distribution


ORPHAN GPCR
(highest levels, relative to other tissues in the dot-blot)







hARE-3
Cerebellum left, Cerebellum right, Testis, Accumbens


hGPCR3
Corpus collusum, Caudate nucleus, Liver, Heart, Inter-



Ventricular Septum


hARE-2
Cerebellum left, Cerebellum right, Substantia


hCHN8
Cerebellum left, Cerebellum right, Kidney, Lung









2. RT-PCR
a. hRUP3
To ascertain the tissue distribution of hRUP3 mRNA, RT-PCR was performed using hRUP3-specific primers and human multiple tissue cDNA panels (MTC, Clontech) as templates. Taq DNA polymerase (Stratagene) was utilized for the PCR reaction, using the following reaction cycles in a 40 ul reaction: 94° C. for 2 min; 94° C. for 15 sec; 55° C. for 30 sec; 72° C. for 1 min; 72° C., for 10 min. Primers were as follows:









5′-GACAGGTACCTTGCCATCAAG-3′
(SEQ.ID.NO.: 61;




sense)


 


5′-CTGCACAATGCCAGTGATAAGG-3′
(SEQ.ID.NO.: 62;



antisense).






20 ul of the reaction was loaded onto a 1% agarose gel; results are set forth in FIG. 3.

As is supported by the data of FIG. 3, of the 16 human tissues in the cDNA panel utilized (brain, colon, heart, kidney, lung, ovary, pancreas, placenta, prostate, skeleton, small intestine, spleen, testis, thymus leukocyte, and liver) a single hRUP3 band is evident only from the pancreas. Additional comparative analysis of the protein sequence of hRUP3 with other GPCRs suggest that hRUP3 is related to GPCRs having small molecule endogenous ligand such that it is predicted that the endogenous ligand for hRUP3 is a small molecule.
b. hRUP4
RT-PCR was performed using hRUP4 oligo's 8 and 4 as primers and the human multiple tissue cDNA panels (MTC, Clontech) as templates. Taq DNA polymerase (Stratagene) was used for the amplification in a 40 ul reaction by the following cycles: 94° C. for 30 seconds, 94° C. for 10 seconds, 55° C. for 30 seconds, 72° C. for 2 minutes, and 72° C. for 5 minutes with cycles 2 through 4 repeated 30 times.
20 μ l of the reaction were loaded on a 1% agarose gel to analyze the RT-PCR products, and hRUP4 mRNA was found expressed in many human tissues, with the strongest expression in heart and kidney. (see, FIG. 4). To confirm the authenticity of the PCR fragments, a 300 bp fragment derived from the 5′ end of hRUP4 was used as a probe for the Southern Blot analysis. The probe was labeled with 32P-dCTP using the Prime-It IITM Random Primer Labeling Kit (Stratagene) and purified using the ProbeQuantTM G-50 micro columns (Amersham). Hybridization was done overnight at 42° C. following a 12 hr pre-hybridization. The blot was finally washed at 65° C. with 0.1×SSC. The Southern blot did confirm the PCR fragments as hRUP4.
c. hRUP5
RT-PCR was performed using the following hRUP5 specific primers:









5′-CTGACTTCTTGTTCCTGGCAGCAGCGG-3′
(SEQ.ID.NO.: 63;




sense)


 


5′-AGACCAGCCAGGGCACGCTGAAGAGTG-3′
(SEQ.ID.NO.: 64;



antisense)






and the human multiple tissue cDNA panels (MTC, Clontech) as templates. Taq DNA polymerase (Stratagene) was used for the amplification in a 40 ul reaction by the following cycles: 94° C. for 30 sec, 94° C. for 10 sec, 62° C. for 1.5 min, 72° C. for 5 min, and with cycles 2 through 3 repeated 30 times. 20 μl of the reaction were loaded on a 1.5% agarose gel to analyze the RT-PCR products, and hRUP5 mRNA was found expressed only in the peripheral blood leukocytes (data not shown).

d. hRUP6
RT-PCR was applied to confirm the expression and to determine the tissue distribution of hRUP6. Oligonucleotides used, based on an alignment of AC005871 and GPR66 segments, had the following sequences:









5′-CCAACACCAGCATCCATGGCATCAAG-3′
(SEQ.ID.NO.: 73;




sense),


 


5′-GGAGAGTCAGCTCTGAAAGAATTCAGG-3′
(SEQ.ID.NO.: 74;



antisense)






and the human multiple tissue cDNA panels (MTC, Clontech) were used as templates. PCR was performed using TaqPlus PrecisionTM polymerase (Stratagene; manufacturing instructions will be followed) in a 40 ul reaction by the following cycles: 94° C. for 30 sec; 94° C. 5 sec; 66° C. for 40 sec, 72° C. for 2.5 min, and 72° C. for 7 min. Cycles 2 through 4 were repeated 30 times.

20 ul of the reaction were loaded on a 1.2% agarose gel to analyze the RT-PCR products, and a specific 760 bp DNA fragment representing hRUP6 was expressed predominantly in the thymus and with less expression in the heart, kidney, lung, prostate small intestine and testis. (see, FIG. 5).
References, including but limited to patent applications, that are cited throughout this patent document, unless otherwise indicated, are incorporated herein by reference. Modifications and extension of the disclosed inventions that are within the purview of the skilled artisan are encompassed within the above disclosure and the claims that follow.
Although a variety of Vectors are available to those in the art, for purposes of utilization for both endogenous and non-endogenous human GPCRs, it is most preferred that the Vector utilized be pCMV. This vector was deposited with the American Type Culture Collection (ATCC) on Oct. 13, 1998 (10801 University Blvd., Manassas, Va. 20110-2209 USA) under the provisions of the Budapest Treaty for the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure. The DNA was tested by the ATCC and determined to be. The ATCC has assigned the following deposit number to pCMV: ATCC #203351.

## Claims

1. An isolated G protein-coupled receptor comprising the amino acid sequence of SEQ ID NO: 8.

2. An isolated G protein-coupled receptor encoded by the nucleotide sequence of SEQ ID NO: 7.

3. An isolated G protein-coupled receptor comprising the amino acid sequence of a mutant of SEQ ID NO: 8 comprising a single substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8.

4. A recombinant G protein-coupled receptor comprising the amino acid sequence of SEQ ID NO: 8.

5. A recombinant G protein-coupled receptor encoded by the nucleotide sequence of SEQ ID NO: 7.

6. A recombinant G protein-coupled receptor comprising the amino acid sequence of a mutant of SEQ ID NO: 8 comprising a single substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8.

7. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor comprising the amino acid sequence of SEQ ID NO: 8 linked to a heterologous polypeptide.

8. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor encoded by the nucleotide sequence of SEQ ID NO: 7; wherein said G protein-coupled receptor is linked to a heterologous polypeptide.

9. A G protein-coupled receptor fusion protein comprising the amino acid sequence of a mutant of SEQ ID NO: 8 comprising a single substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8; wherein said G protein-coupled receptor is linked to a heterologous polypeptide.

10. An isolated G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptors is capable of modulating insulin or glucagon levels.

11. An isolated G protein-coupled receptor comprising an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

12. An isolated G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

13. An isolated G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

14. The isolated G protein-coupled receptor according to any one of claims 10, 11, 12, and 13 wherein said isolated G protein-coupled receptor comprises a substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8.

15. The isolated G protein-coupled receptor according to claim 14, wherein said isolated G protein-coupled receptor is a constitutively activated receptor.

16. The isolated G protein-coupled receptor according to claim 14 wherein said isolated G protein-coupled receptor is a human receptor.

17. The isolated G protein-coupled receptor according to any one of claims 10, 11, 12, and 13 wherein said isolated G protein-coupled receptor is a constitutively activated receptor.

18. The isolated G protein-coupled receptor according to any one of claims 10, 11, 12, and 13 wherein said isolated G protein-coupled receptor is a human receptor.

19. A recombinant G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

20. A recombinant G protein-coupled receptor comprising an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

21. A recombinant G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

22. A recombinant G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

23. The recombinant G protein-coupled receptor according to any one of claims 19, 20, 21, and 22, wherein said recombinant G protein-coupled receptor comprises a substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8.

24. The recombinant G protein-coupled receptor according to claim 23, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor.

25. The recombinant G protein-coupled receptor according to claim 23, wherein said recombinant G protein-coupled receptor is a human receptor.

26. The recombinant G protein-coupled receptor according to any one of claims 19, 20, 21, and 22, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor.

27. The recombinant G protein-coupled receptor according to any one of claims 19, 20, 21, and 22, wherein said recombinant G protein-coupled receptor is a human receptor.

28. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor fusion protein is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

29. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor comprising an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor fusion protein is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

30. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor fusion protein is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

31. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor fusion protein is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

32. The G protein-coupled receptor fusion protein according to any one of claims 28, 29, 30, and 31, wherein said G protein-coupled receptor comprises a substitution of lysine for leucine at amino acid position 224 of SEQ ID NO: 8.

33. The G protein-coupled receptor fusion protein according to claim 32, wherein said heterologous polypeptide is a G protein.

34. The G protein-coupled receptor fusion protein according to claim 32, wherein said G protein-coupled receptor is a constitutively activated receptor.

35. The G protein-coupled receptor fusion protein according to claim 32, wherein said G protein-coupled receptor is a human G protein-coupled receptor.

36. The G protein-coupled receptor fusion protein according to any one of claims 28, 29, 30, and 31, wherein said heterologous polypeptide is a G protein.

37. The G protein-coupled receptor fusion protein according to any one of claims 28, 29, 30, and 31, wherein said G protein-coupled receptor is a constitutively activated receptor.

38. The G protein-coupled receptor fusion protein according to any one of claims 28, 29, 30, and 31, wherein said G protein-coupled receptor is a human G protein-coupled receptor.

39. An isolated G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said isolated G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

40. An isolated G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said isolated G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

41. An isolated G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said isolated G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

42. An isolated G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said isolated G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

43. The isolated G protein-coupled receptor according to any one of claims 39, 40, 41, and 42, wherein said isolated G protein-coupled receptor is a human receptor.

44. A recombinant G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

45. A recombinant G protein-coupled receptor comprising an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO: 8, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

46. A recombinant G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

47. A recombinant G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said recombinant G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

48. The recombinant G protein-coupled receptor according to any one of claims 44, 45, 46, and 47, wherein said isolated G protein-coupled receptor is a human receptor.

49. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

50. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor comprising an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO: 8, wherein said G protein-coupled receptor is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

51. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor encoded by a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

52. A G protein-coupled receptor fusion protein comprising a G protein-coupled receptor encoded by a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 7, wherein said G protein-coupled receptor is linked to a heterologous polypeptide, and wherein said G protein-coupled receptor is a constitutively activated receptor, and wherein said G protein-coupled receptor is capable of modulating insulin or glucagon levels.

53. The G protein-coupled receptor fusion protein according to any one of claims 49, 50, 51, and 52, wherein said heterologous polypeptide is a G protein.

54. The G protein-coupled receptor fusion protein according to claim 53, wherein said G protein-coupled receptor is a human G protein-coupled receptor.

55. The G protein-coupled receptor fusion protein according to any one of claims 49, 50, 51, and 52, wherein said G protein-coupled receptor is a human G protein-coupled receptor.