# Patent

## Title
Fused heterocyclic derivatives as S1P modulators

## Abstract

This invention relates to new fused heterocyclic derivatives having affinity to S1P receptors, a pharmaceutical composition containing said compounds, as well as the use of said compounds for the preparation of a medicament for treating, alleviating or preventing diseases and conditions in which any S1P receptor is involved or in which modulation of the endogenous S1P signaling system via any S1P receptor is involved.

## Background
This application is a national stage entry under 35 U.S.C. §371 of PCT/EP2011/061586, filed Jul. 8, 2011, which claims priority of U.S. Provisional Application No. 61/446,541, filed Feb. 25, 2011, U.S. Provisional Application No. 61/362,784, filed Jul. 9, 2010, European Patent Application No. 11156007.4, filed Feb. 25, 2011, and European Patent Application No. 10169107.9, filed Jul. 9, 2010, the disclosures of each of which are incorporated herein by reference in their entirety.
FIELD OF THE INVENTION
This invention relates to new fused heterocyclic derivatives having affinity to S1P receptors, a pharmaceutical composition containing said compounds, as well as the use of said compounds for the preparation of a medicament for treating, alleviating or preventing diseases and conditions in which any S1P receptor is involved or in which modulation of the endogenous S1P signaling system via any S1P receptor is involved.
BACKGROUND OF THE INVENTION
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that mediates a wide variety of cellular responses, such as proliferation, cytoskeletal organization and migration, adherence- and tight junction assembly, and morphogenesis. S1P can bind with members of the endothelial cell differentiation gene family (EDG receptors) of plasma membrane-localized G protein-coupled receptors. To date, five members of this family have been identified as S1P receptors in different cell types, S1P1 (EDG-1), S1P2 (EDG-5), S1P3 (EDG-3), S1P4 (EDG-6) and S1P5 (EDG-8). S1P can produce cytoskeletal re-arrangements in many cell types to regulate immune cell trafficking, vascular homeostasis and cell communication in the central nervous system (CNS) and in peripheral organ systems. It is known that S1P is secreted by vascular endothelium and is present in blood at concentrations of 200-900 nanomolar and is bound by albumin and other plasma proteins. This provides both a stable reservoir in extracellular fluids and efficient delivery to high-affinity cell-surface receptors. S1P binds with low nanomolar affinity to the five receptors S1P1-5. In addition, platelets also contain S1P and may be locally released to cause e.g. vasoconstriction. The receptor subtypes S1P1, S1P2 and S1P3 are widely expressed and represent dominant receptors in the cardiovascular system. Further, S1P1 is also a receptor on lymphocytes. S1P4 receptors are almost exclusively in the haematopoietic and lymphoid system. S1P5 is primarily (though not exclusively) expressed in central nervous system. The expression of S1P5 appears to be restricted to oligodendrocytes in mice, the myelinating cells of the brain, while in rat and man expression at the level of astrocytes and endothelial cells was found but not on oligodendrocytes.
S1P receptor modulators are compounds which signal as (ant)agonists at one or more S1P receptors. The present invention relates to modulators of the S1P5 receptor, in particular agonists, and preferably to agonists with selectivity over S1P1 and/or S1P3 receptors, in view of unwanted cardiovascular and/or immunomodulatory effects. It has now been found that S1P5 agonists can be used in the treatment of cognitive disorders, in particular age-related cognitive decline.
Although research is ongoing to develop therapeutics that can be used to treat age related cognitive decline and dementia, this has not yet resulted in many successful candidates. Therefore, there is a need for new therapeutics with the desired properties.
DESCRIPTION OF THE INVENTION
It has now been found that fused heterocyclic derivatives of the formula (I)

    
    
        A fused heterocyclic derivative of the formula (I)
    
    






    
    
        wherein
        R1 is selected from
        
            cyano,
            (2-4C)alkenyl, (2-4C)alkynyl, (1-4C)alkyl, each optionally substituted with CN or one or more fluoro atoms,
            (3-6C)cycloalkyl, (4-6C)cycloalkenyl or a (8-10C)bicyclic group, each optionally substituted with halogen or (1-4C)alkyl optionally substituted with one or more fluoro atoms,
            phenyl, biphenyl, naphthyl, each optionally substituted with one or more substituents independently selected from halogen, cyano, (1-4C)alkyl optionally substituted with one or more fluoro atoms, (1-4C)alkoxy optionally substituted with one or more fluoro atoms, amino, dimethylamino, and (3-6C)cycloalkyl optionally substituted with phenyl which may be substituted with (1-4C)alkyl or halogen, and
            phenyl substituted with phenoxy, benzyl, benzyloxy, phenylethyl or monocyclic heterocycle, each optionally substituted with (1-4C)alkyl,
        
        
        Z is a linking group —W—(Cn-alkylene)-T- wherein
        
            W is attached to R1 and selected from a bond, —O—, —CO—, —S—, —SO—, —SO2—, —NH—, —CH═CH—, —C(CF3)═CH—, —C≡C—, —CH2—O—, —O—CO—, —CO—O—, —CO—NH—, —NH—CO— and trans-cyclopropylene;
            n is an integer from 0 to 10; and
            T is attached to the phenylene/pyridyl moiety and selected from a bond, —O—, —S—, —SO—, —SO2—, —NH—, —CO—, —C═C—, —C≡C—, and trans-cyclopropylene;
        
        
        R2 is H or one or more substituents independently selected from cyano, halogen, (1-4C)alkyl optionally substituted with one or more halogen atoms, or (1-4C)alkoxy optionally substituted with one or more halogen atoms;
        ring structure A may contain one nitrogen atom;
        X is selected from C or N; if X is C, R3 is selected from H and (1-4C)alkyl, otherwise R3 is not present;
        Y is selected from NH, O and S;
        structure Q is a 5-, 6- or 7-membered cyclic amine; and
        R4 is (1-4C)alkylene-R5 wherein one or more carbon atoms in the alkylene group may independently be substituted with one or more halogen atoms or with (CH2)2 to form a cyclopropyl moiety, or R4 is (3-6C)cycloalkylene-R5, —CH2-(3-6C)cycloalkylene-R5, (3-6C)cycloalkylene-CH2—R5 or —CO—CH2—R5, wherein R5 is —OH, —PO3H2, —OPO3H2, —COOH, —COO(1-4C)alkyl or tetrazol-5-yl;

or a pharmaceutically acceptable salt, a solvate or hydrate thereof or one or more N-oxides thereof display affinity for S1P receptors. In particular, compounds of the invention show selective affinity for the S1P5 receptor over the S1P1 and/or S1P3 receptor(s).

    
    


In WO 2008/012010, some of the disclosed compounds have somewhat structural similarity to the compounds of the present invention; however, they are described as histamine H3-receptor ligands.
The compounds of the invention are modulators of the S1P receptor, in particular of the S1P5 receptor. More specifically, the compounds of the invention are S1P5 receptor agonists. The compounds of the invention are useful for treating, alleviating and preventing diseases and conditions in which (any) SIP receptor(s)—in particular S1P5—is (are) involved or in which modulation of the endogenous S1P signaling system via any S1P receptor is involved. In particular, the compounds of the present invention may be used to treat, alleviate or prevent CNS (central nervous system) disorders, such as neurodegenerative disorders, in particular—but not limited to—cognitive disorders (in particular age-related cognitive decline) and related conditions, Alzheimer's disease, (vascular) dementia, Nieman's Pick disease, and cognitive deficits in schizophrenia, obsessive-compulsive behavior, major depression and autism, multiple sclerosis, pain, etc. Preferably, the compounds of the present invention may be used to treat, alleviate or prevent cognitive disorders (in particular age-related cognitive decline) and related conditions.
In a preferred embodiment of the invention, the compounds have formula (I)
wherein

    
    
        R1 is selected from
        
            (3-6C)cycloalkyl or a (8-10C)bicyclic group optionally substituted with halogen, (1-4C)alkyl, and
            phenyl optionally substituted with one or more substituents independently selected from halogen, cyano, (1-4C)alkyl, (1-4C)alkoxy, trifluoromethyl and trifluoromethoxy;
        
        
        W is selected from a bond, —O—, —CO—, —S—, —SO—, —SO2—, —NH—, —CH═CH—, —C≡C—, and trans-cyclopropylene; and
        n is an integer from 0 to 4; and preferably, n is selected from 0, 1 and 2; and
        R2 is H or one or more substituents independently selected from halogen, (1-4C)alkyl optionally substituted with one or more fluoro atoms or (1-4C)alkoxy optionally substituted with one or more fluoro atoms; and wherein the definition of the other groups/symbols is as defined previously.
    
    


In another embodiment, the compound of the invention has the structure (Ia)




In an embodiment of the invention, ring structure Q is a 6-membered ring. In particular, the compound of the invention has the structure (Ib)




In a further embodiment of the invention, R4 is selected from —(CH2)2—OH, —CH2—COOH, —(CH2)2—COOH, —(CH2)3—COOH, —CH2—CHCH3—COOH, —CH2—C(CH3)2—COOH, —CHCH3—CH2—COOH, —CH2—CF2—COOH, —CO—CH2—COOH,




1,3-cyclobutylene-COOH, —(CH2)2—PO3H2, —(CH2)3—PO3H2, —(CH2)2—OPO3H2, —(CH2)3—OPO3H2, —CH2-tetrazol-5-yl, —(CH2)2-tetrazol-5-yl and —(CH2)3-tetrazol-5-yl. Preferred R4 groups are selected from —(CH2)2—COOH, —(CH2)3—COOH, —CH2—CHCH3—COOH, —CH2—C(CH3)2—COOH, —CHCH3—CH2—COOH, —CH2—CF2—COOH and. Highly preferred are —(CH2)2—COOH, —CHCH3—CH2—COOH, —CH2—CHCH3—COOH and 1,3-cyclobutylene-COOH. In particular preferred is —CH2—CHCH3—COOH.

In another preferred embodiment, the compounds have formula (I) wherein Y is O.
Further, in a preferred embodiment of the invention, X is N.
In preferred embodiments of the invention, R1 is indanyl optionally substituted with halogen, (1-4C)alkyl, or—more preferred—R1 is optionally substituted phenyl, wherein the optional substituents are selected from any of the previously defined substituents, but in particular the optional substituents are one or more substituents independently selected from halogen, cyano, (1-4C)alkyl, (1-4C)alkoxy, trifluoromethyl and trifluoromethoxy. In highly preferred embodiments, R1 is 4Cl-phenyl or 4CF3-phenyl.
In an embodiment of the invention, R2 is H or one or more substituents independently selected from methyl, methoxy, chloro or fluoro. In a preferred embodiment, R2 is H or R2 represents one methyl, one methoxy, one chloro, one chloro, or one or two fluoro atoms.
In embodiments of the invention, wherein X is CR3, R3 is preferably H or methyl and in particular, R3 is H.
Further, in an embodiment of the invention, Z is the linking group —W—(CH2)n-T-, the meaning of which is selected from a bond, —O—, —CO—, —S—, —SO2—, —NH—, —CH2—, —(CH2)2—, —CCH3—O—, —CH═CH—, —C≡C—, —CH2—O—, —O—CH2—, —CH2—S—, —S—CH2—, —CH2—SO2—, —SO2—CH2—, —CH2—NH—, —NH—CH2—, and trans-cyclopropylene. In preferred embodiments, Z is —O—, —CH2—O— or trans-cyclopropylene. In particular, Z is —CH2—O—.
The term halogen refers to fluoro, chloro, bromo, or iodo. Preferred halogens are fluoro and chloro, and in particular chloro.
The term (1-4C)alkyl means a branched or unbranched alkyl group having 1-4 carbon atoms, for example methyl, ethyl, propyl, isopropyl and butyl. A preferred alkyl group is methyl.
The term (1-4C)alkoxy means an alkoxy group having 1-4 carbon atoms, wherein the alkyl moiety is as defined above. A preferred alkoxy group is methoxy.
The terms (1-4C)alkylene and (Cn-alkylene) mean a branched or unbranched alkylene group having 1-4 or n carbon atoms, respectively, for example methylene, —CHCH3—, —C(CH3)2—, —CHCH3CH2—, and the like. In the definition of R4 which is (1-4C)alkylene-R5, one or more carbon atoms in the alkylene group may (amongst others) independently be substituted with (CH2)2 to form a cyclopropyl moiety, meaning to form a R4 group such as




The term (2-4C)alkynyl means a branched or unbranched alkynyl group having 2-4 carbon atoms, wherein the triple bond may be present at different positions in the group, for example ethynyl, propargyl, 1-butynyl, 2-butynyl, etc.

The term (3-6C)cycloalkyl means a cyclic alkyl group having 3-6 carbon atoms, thus cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl. Preferred are cyclopentyl and cyclohexyl.
The term (4-6C)cycloalkenyl means a cyclic alkenyl group having 4-6 carbon atoms and comprising one or two double bonds, for example cyclohexenyl.
The term (3-6C)cycloalkylene means a cyclic alkyl group having two attachment points. Preferred is 1,3-cyclobutylene, having the structure




The term (8-10C)bicyclic group means a fused ring system of two groups selected from aromatic and non-aromatic ring structures having together 8-10 carbon atoms, for example the indane group.
With reference to substituents, the term “independently” means that the substituents may be the same or different from each other in the same molecule.
The compounds of the invention may suitably be prepared by methods available in the art, and as illustrated in the experimental section of this description.
The compounds of the present invention may contain one or more asymmetric centers and can thus occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. Additional asymmetric centers may be present depending upon the nature of the various substituents on the molecule. Each such asymmetric center will independently produce two optical isomers and it is intended that all of the possible optical isomers and diastereomers in mixtures and as pure or partially purified compounds are included within the ambit of this invention. The present invention is meant to comprehend all such isomeric forms of these compounds. The independent syntheses of these diastereomers or their chromatographic separations may be achieved as known in the art by appropriate modification of the methodology disclosed herein. Their absolute stereochemistry may be determined by the x-ray crystallography of crystalline products or crystalline intermediates which are derivatized, if necessary, with a reagent containing an asymmetric center of known absolute configuration. If desired, racemic mixtures of the compounds may be separated so that the individual enantiomers are isolated. The separation can be carried out by methods well known in the art, such as the coupling of a racemic mixture of compounds to an enantiomerically pure compound to form a diastereomeric mixture, followed by separation of the individual diastereomers by standard methods, such as fractional crystallization or chromatography.
Compounds may exist as polymorphs and as such are intended to be included in the present invention. In addition, compounds may form solvates with water (i.e., hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention.
Isotopically-labeled compound of formula (I) or pharmaceutically acceptable salts thereof, including compounds of formula (I) isotopically-labeled to be detectable by PET or SPECT, also fall within the scope of the invention. The same applies to compounds of formula (I) labeled with [13C]—, [14C]—, [3H]—, [18F]—, [125I]— or other isotopically enriched atoms, suitable for receptor binding or metabolism studies.
The term “pharmaceutically acceptable salt” refers to those salts that are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well-known in the art. They can be prepared in situ when isolating and purifying the compounds of the invention, or separately by reacting them with pharmaceutically acceptable non-toxic bases or acids, including inorganic or organic bases and inorganic or organic acids.
The compounds of the invention may be administered enterally or parenterally. The exact dose and regimen of these compounds and compositions thereof will be dependent on the biological activity of the compound per se, the age, weight and sex of the patient, the needs of the individual subject to whom the medicament is administered, the degree of affliction or need and the judgment of the medical practitioner. In general, parenteral administration requires lower dosages than other methods of administration which are more dependent upon adsorption. However, the dosages for humans are preferably 0.001-10 mg per kg body weight. In general, enteral and parenteral dosages will be in the range of 0.1 to 1,000 mg per day of total active ingredients.
Mixed with pharmaceutically suitable auxiliaries, e.g. as described in the standard reference “Remington, The Science and Practice of Pharmacy” (21st edition, Lippincott Williams & Wilkins, 2005, see especially Part 5: Pharmaceutical Manufacturing) the compounds may be compressed into solid dosage units, such as pills or tablets, or be processed into capsules or suppositories. By means of pharmaceutically suitable liquids the compounds can also be applied in the form of a solution, suspension or emulsion.
For making dosage units, e.g. tablets, the use of conventional additives such as fillers, colorants, polymeric binders and the like, is contemplated. In general, any pharmaceutically suitable additive which does not interfere with the function of the active compounds can be used.
Suitable carriers with which the compounds of the invention can be administered include for instance lactose, starch, cellulose derivatives and the like, or mixtures thereof, used in suitable amounts. Compositions for intravenous administration may for example be solutions of the compounds of the invention in sterile isotonic aqueous buffer. Where necessary, the intravenous compositions may include for instance solubilizing agents, stabilizing agents and/or a local anesthetic to ease the pain at the site of the injection.
Pharmaceutical compositions of the invention may be formulated for any route of administration and comprise at least one compound of the present invention and pharmaceutically acceptable salts thereof, with any pharmaceutically suitable ingredient, excipient, carrier, adjuvant or vehicle.
By “pharmaceutically suitable” it is meant that the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
In an embodiment of the invention, a pharmaceutical pack or kit is provided comprising one or more containers filled with one or more pharmaceutical compositions of the invention. Associated with such container(s) can be various written materials such as instructions for use, or a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals products, which notice reflects approval by the agency of manufacture, use, or sale for human or veterinary administration.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described in this document.



LEGEND TO THE FIGURES
FIG. 1 Percentage of alternation of young and old C57BL/6J male mice in the T-maze with either vehicle (control groups) or compound of the invention (dose in mg/kg; p.o.)



The following examples are intended to further illustrate the invention in more detail.
Any novel intermediate as disclosed herein is a further embodiment of the present invention.
EXAMPLES
§1. Analytical Methods
Nuclear magnetic resonance spectra (1H NMR and 13C NMR, APT) were determined in the indicated solvent using a Bruker ARX 400 (1H: 400 MHz, 13C: 100 MHz) at 300K, unless indicated otherwise. 19F NMR and 13C NMR experiments were carried out on a Varian Inova 500 spectrometer operating at 11.74 T (499.9 MHz for 1H, 125.7 MHz for 13C, 50.7 Mhz, 470.4 MHz for 19F) using a 5 mm SW probe. The spectra were determined in deuterated chloroform or DCM obtained from Cambridge Isotope Laboratories Ltd. Chemical shifts (6) are given in ppm downfield from tetramethylsilane (1H, 13C) or CCl3F (19F). Coupling constants J are given in Hz. Peak shapes in the NMR spectra are indicated with the symbols ‘q’ (quartet), ‘dq’ (double quartet), ‘t’ (triplet), ‘dt’ (double triplet), ‘d’ (doublet), ‘dd’ (double doublet), ‘s’ (singlet), ‘bs’ (bs) and ‘m’ (multiplet). NH and OH signals were identified after mixing the sample with a drop of D2O.
Flash chromatography refers to purification using the indicated eluent and silica gel (either Acros: 0.030-0.075 mm or Merck silica gel 60: 0.040-0.063 mm).
Column chromatography was performed using silica gel 60 (0.063-0.200 mm, Merck).
Reactions were monitored by using thin-layer chromatography (TLC) on silica coated plastic sheets (Merck precoated silica gel 60 F254) with the indicated eluent. Spots were visualized by UV light (254 nm) or 12.
Melting points were recorded on a Büchi B-545 melting point apparatus.
Liquid Chromatography-Mass Spectrometry (LC-MS):
Method A.
The LC-MS system consists of a Waters 1525 μ-pump. The pump is connected to a Waters 2777 auto sampler.
The LC method is:
















flow




step
total time
(ul/min)
A (%)
B (%)



















0
0.2
1600
90
10


1
2.5
1600
0
100


2
2.8
1600
0
100


3
2.9
1600
90
10


4
3.10
1600
90
10


5
3.11
500
90
10





A = 100% Water with 0.2% HCOOH


B = 100% ACN with 0.2% HCOOH






The auto sampler has a 10 ul injection loop; the injection volume is 101. The auto sampler is connected to a Waters Sunfire C18 30*4.6 mm column with 2.5 um particles. The column is thermo stated at Room temperature +/−23° C.
The column is connected to a Waters 2996 PDA. The wavelength is scanned from 240 to 320 nm. The resolution is 1.2 nm and the sampling ate is 20 Hz. After the PDA the flow is split 1:1 and connected to a Waters 2424 ELSD.
The ELSD has the following parameters:
Gas pressure: 40 psi
Data rate 20 points/sec
Gain 500
Time constant 0.2 sec
Nebulizer mode cooling
Drift tube 50° C.
The samples are also measured with a Waters ZQ mass detector.
The mass spectrometer has the following parameters:
Scan range: 117-900 Amu
Polarity: positive
Data format: centroid
Time per scan: 0.500 sec
Interscan time: 0.05 sec
















Capillary
2.5
kV



Cone
25
V



Extractor
2
V



RF lens
0.5
V



Source Temp.
125°
C.



Desolvation Temp
400°
C.



Cone gas
100
L/Hr



Desolvation Gas
800
L/Hr










LM 1 Resolution
15



HM 1 Resolution
15



Ion energy
0.5











Multiplier
500
V









The complete system is controlled by Masslynx 4.1.
Method B.
The LC-MS system consists of 2 Perkin Elmer series 200 micro pumps. The pumps are connected to each other by a 50 ul tee mixer. The mixer is connected to the Gilson 215 auto sampler.
The LC method is:















total
flow




step
time
(ul/min)
A (%)
B (%)



















0
0
1800
95
5


1
1.8
1800
0
100


2
2.6
1800
0
100


3
2.8
1800
95
5


4
3.0
1800
95
5





A = 100% Water with 0.1% HCOOH


B = 100% Acetonitril with 0.1% HCOOH






The auto sampler has a 2 ul injection loop. The auto sampler is connected to a Waters Sunfire C18 4.6×30 mm column with 2.5 □m particles. The column is thermo stated in a Perkin Elmer series 200 column oven at 23° C. The column is connected to a Perkin Elmer 785 UV/VIS meter with a 2.7 ul flow cell. The wavelength is set to 254 nm. The UV meter is connected to a Sciex API 150EX mass spectrometer. The mass spectrometer has the following parameters:
Scan range: 100-900 Amu
Polarity: positive
Scan mode: profile
Resolution Q1: UNIT
Step size: 0.10 amu
Time per scan: 0.500 sec
NEB: 10
CUR: 10
IS: 5200
TEM: 325
DF: 30
FP: 225
EP: 10
The light scattering detector is connected to the Sciex API 150. The light scattering detector is a Polymer Labs PL-ELS 2100 operating at 70° C. and 1.7 bar N2 pressure.
The complete system is controlled by a Dell precision GX370 computer operating under Windows 2000.
The reported retention times in Table 1 (Rt) are for the peak in the Total Ion Current (TIC) chromatogram which showed the mass for [M+H]+ within 0.5 amu accuracy of the calculated exact MW and had an associated peak in the Evaporative Light Scattering (ELS) chromatogram with a relative area % (purity) of >85%.
§2. Abbreviations
















ACE-Cl 
1-Chloroethyl chloroformate



9-BBN
9-borabicyclo[3.3.1]nonane dimer



CHCl3
Chloroform



CH2Cl2 
Dichloromethane



CH3CN
Acetonitrile



CuBr2
Copper(II) bromide



DBU
1,8-Diazabicyclo[5.4.0]undec-7-ene



DIAD
Diisopropyl azodicarboxylate



DIPEA
N,N-Diisopropylethylamine



DMF
N,N-dimethylformamide



DMSO
Dimethyl sulfoxide



Et3N
Triethylamine



Et2O
Diethyl ether



EtOH
Ethanol



EtOAc
Ethyl acetate



HCl
Hydrogen chloride



K2CO3
Potassium carbonate



KHCO3
Potassium bicarbonate



KI
Potassium iodide



KOH
Potassium hydroxide



KOtBu
Potassium tert-butoxide



MeOH
Methanol



NaBH4
Sodium borohydride



NaHCO3 
Sodium bicarbonate



NaI
Sodium iodide



NaOH
Sodium hydroxide



NaOtBu
Sodium tert-butoxide



Na2SO4
Sodium sulfate



NBS
N-Bromosuccinimide



iPr2O
Diisopropyl ether



RT
Room Temperature



SiO2
Silica gel



TFA
Trifluoroacetic acid



THF
Tetrahydrofuran



TMSCl
Chlorotrimethylsilane



TMSOTf 
Trimethylsilyl trifluoromethanesulfonate










§3. General Aspects of Syntheses
Suitable syntheses of claimed compounds are described below.




For the synthesis of compounds 1, two routes are described in respectively Schemes 2 and 3. Both routes start with compound 6, the synthesis of which is depicted in Scheme 1. Alpha alkylation of the pyrrolidine-enamine of 4 with alpha-bromo-acetophenones (3)-thereby introducing the Rb—group in the molecule—gives compound 5. Subsequent ring-closure of 5 under acidic conditions yielded compound 6 in fair yields.




Route A (see Scheme 2) starts with the alkylation of the piperidine moiety in 6 by either a standard alkylation, reductive alkylation or Michael addition reaction to give the protected carboxylic acid compounds 7. The benzyl ether moiety could be introduced in two ways. Firstly, the bromine in 7 could be converted directly to the benzyl ether derivative 9 by a palladium catalyzed reaction. Additionally, bromide 7 can be converted to the phenol derivative 8 derivative via a palladium mediated reaction. Compound 8 can be converted to the desired benzyl ether derivatives 9 under phase transfer conditions with benzyl bromides or via a Mitsunobu reaction with benzyl alcohols. Finally, compounds 9 could be deprotected to give the end-products 1.




Alternatively, Route B (see Scheme 3) could be followed for the synthesis of compounds 1. The piperidine in compound 6 was protected with a BOC group. Hereafter, first the benzyl ether moiety was introduced either via a direct palladium mediated reaction of the bromine in 10 to 12 or via transforming the bromine to the phenol derivative 11, which could be converted to 12 under alkylation or Mitsunobu conditions. Finally, compound 12 could be converted to 9 by acidic removal of the BOC group and subsequent introduction of the protected carboxylic acid tails.




For the synthesis of oxazolo-derivatives 2, three routes were developed. The synthesis of key-intermediate 20 is depicted in Scheme 4. Acylation of commercially available 14 with a properly substituted benzoyl chloride (15) gave 16, which was subsequently ring-closed to 17 by using triphenylphosphine and hexachloroethane. Methylation of the pyridine in 17 to the quaternary salt 18 and subsequent reduction of 18 with sodium borohydride yielded compound 19. Compound 19 was demethylated with 1-chloroethyl chloroformate to furnish key-intermediate 20.




The first route (Route C) to compounds 2 is outlined in Scheme 5 and starts from compound 20. In a similar fashion as described for the synthesis of compounds 7 in the furanyl series, the t-butyl protected carboxylic acid tails could be introduced in 20 to give 21. Starting from 21, the benzyl ether derivatives 23 could be prepared by either a direct palladium mediated coupling (21 to 23) with benzyl alcohols or by first transforming the bromide in 21 to phenol 22 and subsequent benzylation of 22 (to 23) under phase transfer or Mitsunobu conditions. Finally, acidic deprotection of the carboxylic acid in 23 yielded compounds 2.




Alternatively, route D could be followed as depicted in Scheme 6. Compounds 25 could be prepared starting from 14 and a properly substituted 4-benzyloxy-benzoic acid derivative (24) under the influence of triphenylphosphine and trichloroacetonitril. Compound 25 could be converted to the benzyloxy-derivatives 23 in a similar fashion as described above in Schemes 4 and 5 for the synthesis of compounds 21. Thus, methylation of 25 and subsequent reduction with NaBH4 gave 26, which was demethylated with ACE-C1 to give 27. Finally, the tails were introduced in 27 to give compound 23. From here, the t-butyl group in 23 could be removed under acidic conditions to give compound 2. On the other hand, the benzyl in 23 can be removed by hydrogenation to give phenol derivatives 22.




And finally, the third route (Route E) to compounds 2 is depicted in Scheme 7. Compound 20 was protected with a t-butyloxycarbonyl group to give 28, which could be converted to the corresponding phenol (29) under standard palladium conditions. Alkylation of 29 under phase transfer or Mitsunobu conditions gave 30. On the other hand, compound 30 could also be obtained directly from the bromide 28 under palladium chemistry conditions. Acidic removal of the BOC group in 30 resulted in the formation of compound 27, which could be alkylated to 23 as described in Scheme 5.











Thiazolo derivatives 508 and 520 were synthesized as described in Scheme 8 and 9. Adjustments of R-groups in the reagents leads to the introduction of Ra, Rb and Rc.







The synthetic route towards a number of alternative tails and linkers is depicted in Scheme 10.







For those skilled in the art, it is clear that the choice for a certain route can be based on the availability of the reagents. In addition, the routes B, D and E are very suitable for the introduction of diversity in the Rc-tail part of compounds 1 and 2. Routes A and C have the introduction of the Ra-Bn moiety in the last part of the synthesis which makes it more suitable for exploring diversity in that part of the molecule.
§4. Syntheses of Intermediates
General Procedure for the Synthesis of Compounds 5.
To a solution of 4-oxo-piperidine-1-carboxylic acid t-butyl ester in toluene (2 ml/mmol) was added a catalytic amount of para-toluenesulphonic acid mono hydrate (0.1 eq) and pyrrolidine (4 eg). The mixture was heated to reflux under Dean Stark conditions for 18 hours. The mixture was concentrated under reduced pressure and the residue was redissolved in toluene. To this solution was added slowly (in 25 minutes) a solution of a properly substituted 2-bromo-1-(4-bromo-phenyl)-ethanone (1.05 eq) in toluene/DCM (2 ml/mmol, 1/2, v/v). The mixture was stirred overnight at room temperature and the resulting white slurry was poured out into water. The water layer was extracted with DCM (3 times) and the combined organic layers were dried (MgSO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography giving compound 5 in a yield of 50-90%.
General Procedure for the Synthesis of Compounds 6.
Compound 5 was suspended in concentrated hydrochloric acid (10 eq, 12N). The mixture was heated (in steps of 10° C. per 30 minutes) to 80° C. The mixture starts to foam heavily, so allow enough volume in the starting reaction vessel. After 45 minutes, the mixture was cooled to 0° C. and neutralized with 50 wt % solution of NaOH (exothermic). After stirring overnight at room temperature, the resulting solid material was collected by filtration and washed with 0.1M NaOH. The light brown material was purified by Soxhlet extraction in EtOAc giving 6 as beige solid which was used in the next step without further purification.
General Procedure for the Introduction of the Protected Carboxylic Acid Tails (7).
a) Introduction of the Propionic Acid t-Butyl Ester.
Compound 6 was suspended in methanol (4 ml/mmol) and DIPEA was added (1.05 eg). To the mixture was added t-butyl acrylate (1.2 eq) and the mixture was refluxed for 16 hrs. Conversion was checked by TLC analysis. The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 7a.
b) Introduction of the 2-Methyl-Propionic Acid t-Butyl Ester.
Compound 6 was suspended in DMF (6 ml/mmol). To this suspension was added 1,8-diazabicyclo[5.4.0]undec-7-ene (3 eg) and t-butyl methacrylate (2 eq). The mixture was heated at 125° C. for 16-100 hrs. The solution was cooled and 5% NaHCO3 was added (15 ml/mmol) and extracted with EtOAc. The organic layer was washed with water (4×), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 7b.
c) Introduction of the 3-Butyric Acid t-Butyl Ester.
Compound 6 was suspended in 1,2-dichloroethane (6 ml/mmol). To this suspension was added t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq). The mixture was stirred at room temperature for 16 hrs. If the reaction was not complete, another portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) was added. To the solution was added 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish pure 7c.
d)) Introduction of the 4-Butyric Acid t-Butyl Ester.
Compound 6 was suspended in acetonitril (3 ml/mmol). To this suspension was added potassium carbonate (2 eq), t-butyl 4-bromo-butanoate (1.1 eq) and potassium iodide (1.1 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo and the residue was dissolved in EtOAc, washed with 5% NaHCO3 (15 ml/mmol). The organic layer was dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to yield 7d.
General Procedure for the Introduction of the Benzyl Ether Moiety in 7 to Compound 9.
A solution of compound 7, the properly substituted benzyl alcohol (1.1 eq), palladium(II) acetate (0.02 eq), 2-dit-butylphosphino-3,4,5,6-tertamethyl-2′,4′,6′-triisopropyl-1,1′biphenyl (0.02 eq), cesium carbonate (1.5 eq) in degassed toluene (4 ml/mmol) was heated at 75° C. for 16 hrs. Conversion was checked by TLC analysis. The solution was cooled to room temperature, diluted with DCM, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 9 in yields varying from 30-80%.
General Procedure for the Conversion of the Bromine Derivatives 7 to the Phenol Derivatives 8.
Compound 7 was dissolved in toluene (8 mml/mmol) and to the solution was added potassium hydroxide (2 eq, 11.7N) and the solution was degassed. To the solution was added 2-dit-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.03 eq). The mixture was stirred at 60° C. for 1.25 hrs. The mixture was allowed to reach room temperature, diluted with EtOAc and washed with 5% NaHCO3 solution (10 ml/mmol). The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 8 in yields varying from 25-85%.
General Procedure for the Conversion of the Phenol 8 to Benzyl Ethers 9.
Method A) Compound 8 was dissolved in DCM/water, 2/1, v/v (4 ml/mmol) and to this solution was added sodium hydroxide (2N, 3 eq). To this mixture were added tetrabutylammonium bromide (0.1 eq) and the properly substituted benzyl bromide (1.1 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with DCM (15 ml/mmol), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 9 in yields varying from 80-90%.
Method B) Compound 8 was dissolved in N,N′-dimethylacetamide (4 ml/mmol) and to this solution was added triphenylphosphine (1.25 eq) and diisopropyl azodicarboxylate (1.25 eq) and the properly substituted benzyl alcohol (1.2 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis showed complete reaction. The mixture was diluted with diethyl ether and washed with water (3×). The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 9 in yields varying from 70-90%.
General Procedure for the Acidic Deprotection of Compounds 9 to 1.
Compound 9 was dissolved in a solution of HCl in 1,4-dioxan (4N, 45 eq) and the mixture was stirred at room temperature for 24 hrs. Heating at 50° C. was applied when needed to push the reaction to completion. The solvents were evaporated and diisopropyl ether was added to precipitate the product. The white solid material was filtered and dried in vacuo to give compound 1 in a yield varying from 80-100%.
General Procedure for the Synthesis of the BOC Protected Derivatives of 6.
To a suspension of compound 6 in DCM (6 ml/mmol) were added DIPEA (1 eq), dimethylamino pyridine (DMAP, 0.05 eq) and di-t-butyl dicarbonate (1.1 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The reaction mixture was washed with 5% ag. NaHCO3 solution and the resulting water layers were extracted with DCM. The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 10 in yields varying from 70-90%.
General Procedure for the Synthesis of Compounds 11.
Compound 10 was dissolved in 1,4-dioxan/water, 1/1, v/v (2 ml/mmol) and to the solution was added potassium hydroxide (4 eq, 11.7N) and the solution was degassed. To the solution was added 2-dit-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.04 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.02 eq). The mixture was stirred at 80° C. for 16 hrs. The mixture was cooled to room temperature, diluted with EtOAc, acidified to pH 6 with 0.1N HCl and extracted with EtOAc. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give pure compound 11 in yields varying from 60-95%.
General Procedure for the Synthesis of the Benzyl Ether Derivatives 12.
Method A) Compound 11 was dissolved in DCM/water, 2/1, v/v (4 ml/mmol) and to this solution was added sodium hydroxide (2N, 3 eq). To this mixture were added tetrabutylammonium bromide (0.1 eq) and the properly substituted benzyl bromide (1.1 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with DCM (15 ml/mmol), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 12 in yields varying from 80-90%.
Method B) Compound 11 was dissolved in N,N′-dimethylacetamide (4 ml/mmol) and to this solution was added triphenylphosphine (1.25 eq), diisopropyl azodicarboxylate (DIAD, 1.25 eq) and a properly substituted benzyl alcohol (1.2 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with diethyl ether and washed with water (3×). The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 12 in yields varying from 70-90%. Method C) Compound 10 was dissolved in toluene (8 mml/mmol) and to the solution was added potassium hydroxide (2 eq, 11.7N) and the solution was degassed. To the solution was added properly substituted benzyl bromide (1.1 eq), 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.03 eq). The mixture was stirred at 60° C. for 1.25 hrs. The mixture was cooled to room temperature, diluted with EtOAc and washed with 5% NaHCO3 solution (10 ml/mmol). The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound 12 in yields varying from 30-80%.
General Procedure for the Deprotection of Compounds 12 to 13.
Compound 12 was dissolved in DCM (10 ml/mmol) and trifluoroacetic acid (10 eq) was added. The solution was refluxed for 16 hrs after which time TLC analysis showed complete reaction. The mixture was neutralized with 5% aq. NaHCO3. The mixture was extracted with DCM (3×) and the combined organic layers were washed with brine, dried on Na2SO4 and concentrated in vacuo to give compound 13 which was used in the next step without further purification.
General Procedure for the Introduction of the Protected Carboxylic Acid Tails (9) Starting from Compound 13.
a) Introduction of the Propionic Acid t-Butyl Ester.
Compound 13 was suspended in methanol (4 ml/mmol) and DIPEA was added (1.05 eg). To the mixture was added 1.2 eg of t-butyl acrylate and the mixture was refluxed for 16 hrs. Conversion was checked by TLC analysis. The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 9a.
b) Introduction of the 2-Methyl-Propionic Acid t-Butyl Ester.
To a solution of compound 13 in DMF (6 ml/mmol) in a pyrex bottle was added 1,8-diazabicyclo[5.4.0]undec-7-ene (3 eg) and t-butyl-methacrylate (2 eq). The mixture was heated at 125° C. for 100 hrs. The solution was cooled and 5% NaHCO3 was added (15 ml/mmol) and extracted with diethyl ether/EtOAc, 1/1, v/v. The organic layer was washed with water (4×), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 9b.
c) Introduction of the 3-Butyric Acid t-Butyl Ester.
Compound 13 was suspended in 1,2-dichloroethane (6 ml/mmol). To this suspension was added t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq). The mixture was stirred at room temperature for 16 hrs. If the reaction was not complete, another portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) was added. After complete reaction, the solution was diluted with 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish pure compound 9c.
d)) Introduction of the 4-Butyric Acid t-Butyl Ester.
Compound 13 was suspended in acetonitril (3 ml/mmol). To this suspension were added potassium carbonate (2 eq), t-butyl 4-bromo-butanoate (1.1 eq) and potassium iodide (1.1 eq). The mixture was heated at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo and the residue was dissolved in EtOAc, washed with 5% NaHCO3 (15 ml/mmol). The organic layer was dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to yield compound 9d.
General Procedure for the Synthesis of 2-(4-bromo-phenyl)-oxazolo[4,5-c]pyridine 17.
To a cooled (0° C.) suspension of commercially available 4-hydroxy-3-amino-pyridine in DCM (14, 6 ml/mmol) was added triethyl amine (1.25 eq) and a solution of properly substituted benzoyl chloride 15 (1 eq, 0.3M in DCM). The reaction mixture was allowed to reach room temperature and the mixture was stirred for 16 to 64 hrs after which time TLC analysis showed complete reaction. The mixture was filtered, washed with DCM and ether to furnish 16 as a solid material (50-80% yield) which was used in the next step without further purification. Hexachloroethane (2.5 eq) was dissolved in DCM and triphenyl phosphine (3 eq) and triethyl amine (8 eq) was added. The mixture was stirred for minutes at room temperature and compound 16 was added slowly in 5 equal portions. The mixture was stirred at room temperature for 64 hrs after which time TLC analysis (DCM/MeOH, 97/3, v/v) revealed complete reaction. The solution was concentrated and the residue was suspended in DCM. The mixture was filtered and the residue washed with DCM and diethyl ether to give 17 in a yield of 30-80%.
General Procedure for the Synthesis of Compounds 19.
To a solution of compound 17 in DMF was added iodomethane (4 eg) and the mixture was stirred for 16 hrs. The mixture was concentrated in vacuo and the residue was stirred in EtOAc to give 18 as a white solid. Compound 18 was dissolved in methanol (10 ml/mmol) and the solution was cooled to 0° C. Sodium borohydride (2 eg) was added and the mixture was stirred at 0° C. for 2 hrs after which time it was allowed to reach room temperature and stirring was continued for 16 hrs. Water was added (1 ml/mmol) and the mixture was stirred for 5 minutes. The mixture was co-evaporated with acetonitril and the residue was purified by silica gel column chromatography to yield compound 19 in 50-90%.
General Procedure for the Synthesis of Compounds 20.
To a cooled (0° C.) solution of compound 19 in 1,2-dichloroethane (10 ml/mmol) was added DIPEA (2 eq). At 0° C. 1-chloroethyl chloroformate (3 eq) was added and the mixture was stirred for 10 minutes at 0° C. after which time the temperature was raised to reflux temperature. After 2 hrs, the mixture was concentrated in vacuo and the residue was dissolved in methanol (10 ml/mmol). The solution was stirred for 48 hrs at room temperature. Removal of the solvent resulted in the isolation of compound 20 in a yield of 70-90%.
General Procedure for the Synthesis of Compounds 21.
a) Introduction of the Propionic Acid t-Butyl Ester.
Compound 20 was suspended in methanol (10 ml/mmol) and DIPEA was added (2.05 eg). To the mixture was added 1.2 eg of t-butyl acrylate and the mixture was refluxed for 16 to 120 hrs. Conversion was checked by TLC analysis and when needed additional reagents were added to push the reaction to completion. The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography to give 21a in yields varying from 50-90%
b) Introduction of the 2-Methyl-Propionic Acid t-Butyl Ester.
To a solution of compound 20 in DMF (6 ml/mmol) was added 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU, 3 eg) and t-butylmethacrylate (5 eq). The mixture was heated at 125° C. for 100 hrs. The solution was cooled and 5% NaHCO3 was added (15 ml/mmol) and extracted with diethyl ether/EtOAc, 1/1, v/v. The organic layer was washed with water (4×), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 21b.
c) Introduction of the 3-Butyric Acid t-Butyl Ester.
Compound 20 was suspended in 1,2-dichloroethane (8 ml/mmol). To this suspension were added t-butylacetoacetate (1.4 eq), acetic acid (leg) and sodium triacetoxy borohydride (1.8 eq). The mixture was stirred at room temperature for 16 hrs. If the reaction was not complete, another portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) was added. After complete reaction, the solution was diluted with 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish pure compound 21c.
d)) Introduction of the 4-Butyric Acid t-Butyl Ester.
Compound 20 was suspended in DMF (5 ml/mmol). To this suspension was added potassium carbonate (3 eq) and t-butyl 4-bromobutanoate (3 eq). The mixture was heated at 80° C. for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo and the residue was purified by silica gel column chromatography to yield 21d.
General Procedure for the Synthesis of Compounds 22.
Compound 21 was dissolved in acetonitril (25 mml/mmol) and to the solution was added powdered potassium hydroxide (2 eq) and the solution was degassed. To the solution was added 2-dit-butylphosphino-3,4,5,6-tetramethyl-2′,4′,6′-triisopropyl-1,1-biphenyl (0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.03 eq). The mixture was stirred at 60° C. for 4 hrs. The mixture was cooled to room temperature and concentrated in vacuo. The residue was dissolved in DCM and washed with 0.1M HCl and water. The water layers were extracted with DCM and the combined organic layers was dried on MgSO4. Compound 22 was obtained after silica gel column chromatography in yields varying from 30-70%.
General Procedure for the Introduction of the Benzyl Ether Moiety in 21 to Compound 23.
A solution of compound 21, the properly substituted benzyl alcohol (2 eq), palladium(II) acetate (0.02 eq), 2-dit-butylphosphino-3,4,5,6-tetramethyl-2′,4′,6′-triisopropyl-1,1′biphenyl (0.02 eq), cesium carbonate (1.5 eq) in degassed toluene (3 ml/mmol) was heated at 75° C. for 16 hrs. Conversion was checked by TLC analysis. The solution was cooled to room temperature, diluted with DCM, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 23 in yields varying from 30-80%.
General Procedure for the Conversion of the Phenol 22 to Benzyl Ethers 23.
Method A) Compound 22 was dissolved in DCM/water, 2/1, v/v (4 ml/mmol) and to this solution was added sodium hydroxide (2N, 3 eq). To this mixture were added tetrabutylammonium bromide (0.1 eq) and a properly substituted benzyl bromide (1.1 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with DCM (15 ml/mmol), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 23 in yields varying from 80-90%.
Method B) Compound 22 was dissolved in dry DCM (15 ml/mmol) and to this solution were added triphenylphosphine (1.8 eq) and the properly substituted benzyl alcohol (1.8 eq). To this mixture was added diisopropyl azodicarboxylate (1.8 eq) and the mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 23 in yields varying from 70-90%.
Method C) PS-TBD (3.7 eq.) resin was incubated with a solution of 22 (1.1 eg) in 1 mL of acetonitril for 1.5 h at 50° C. Thereafter, the properly substituted benzyl bromide (1.10 eq.) in acetonitril was added. Subsequently, the reaction mixture was shaken and heated at 75° C. for 16 hrs. Next, the solvent was removed by filtration and the resin was washed with 3×2.5 mL ACN. The combined organics were concentrated in vacuo, followed by flash column chromatography on silica to give compound 23 in yields varying from 60-95%.
General Procedure for the Deprotection of 23 to Compounds 2.
Compound 23 was dissolved in a solution of HCl in 1,4-dioxan (4N, 100 eq) and the mixture was stirred for 16 hrs at room temperature. Heating at 50° C. was applied when needed to push the reaction to completion. The solvents were evaporated and diisopropyl ether was added to precipitate the product. The white solid material was filtered and dried in vacuo to give compound 2 in a yield varying from 70-100%.
General Procedure for the Synthesis of Compounds 25.
To a cooled (0° C.) suspension of commercially available 4-hydroxy-3-amino-pyridine (14) in acetonitril (15 ml/mmol) was added a properly substituted 4-benzyloxy-benzoic acid (24, 1 eq), triphenylphosphine (3 eq) and trichloroacetonitril (3 eq). The reaction mixture was allowed to reach room temperature and the mixture was stirred for 16 to 64 hrs at 80° C. The mixture was concentrated in vacuo and the residues was dissolved in DCM and washed with 2N NaOH (3×). The combined water layers were extracted with DCM and the organic layers dried (Na2SO4) to give crude 25 as oil which was used in the next step without further purification.
General Procedure for the Synthesis of Compounds 26.
To a solution of compound 25 in DMF (5 ml/mmol) was added iodomethane (4 eg) and the mixture was stirred for 16 hrs. The mixture was concentrated in vacuo and the residue was stirred in EtOAc to give the quaternary salt of 25 as a white solid. The crude material was dissolved in methanol (10 ml/mmol) and the solution was cooled to 0° C. Sodium borohydride (2.5 eg) was added and the mixture was stirred at 0° C. for 2 hrs after which time it was allowed to reach room temperature and stirring was continued for 16-64 hrs. Water was added (1 ml/mmol) and the mixture was stirred for 5 minutes. The mixture was concentrated in vacuo, the residues suspended in 2 N NaOH (5 ml/mmol) and extracted with DCM (3×). The combined organic layers were dried (Na2SO4) and concentrated to give crude 26 as a yellow solid which was used in the next step without further purification.
General Procedure to Compounds 27.
To a cooled (0° C.) solution of compound 26 in 1,2-dichloroethane (10 ml/mmol) was added DIPEA (2 eq) and 1-chloroethyl chloroformate (3 eq) was added. The mixture was stirred for 10 minutes at 0° C. after which time the temperature was raised to reflux temperature. After 4 hrs, the mixture was allowed to reach room temperature and stirring was continued for 16 hrs. The mixture was concentrated in vacuo and the residue was dissolved in methanol (10 ml/mmol). The solution was stirred for 16-48 hrs at room temperature. Removal of the solvent resulted in the isolation of crude 27 in an overall yield of 20-40% based on 25.
General Procedure for the Introduction of the Protected Carboxylic Acid Tails (to 23) Starting from Compound 27.
a) Introduction of the Propionic Acid t-Butyl Ester.
Compound 27 was suspended in methanol (4 ml/mmol) and DIPEA was added (1.05 eg). To the mixture was added 1.2 eg of t-butyl acrylate and the mixture was refluxed for 16 hrs. Conversion was checked by TLC analysis. The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 23a.
b) Introduction of the 2-Methyl-Propionic Acid t-Butyl Ester.
To a solution of compound 27 in DMF (6 ml/mmol) was added 1,8-diazabicyclo[5.4.0]undec-7-ene (3 eg) and t-butylmethacrylate (4 eq). The mixture was heated at 125° C. for 100 hrs. The solution was cooled and 5% NaHCO3 was added (15 ml/mmol) and extracted with diethyl ether/EtOAc, 1/1, v/v. The organic layer was washed with water (4×), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to give pure 23b.
c) Introduction of the 3-Butyric Acid t-Butyl Ester.
Compound 27 was suspended in 1,2-dichloroethane (6 ml/mmol). To this suspension was added t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq). The mixture was stirred at room temperature for 16 hrs. If the reaction was not complete, another portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) was added. After complete reaction, the solution was diluted with 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish pure compound 23c.
d)) Introduction of the 4-Butyric Acid t-Butyl Ester.
Compound 27 was suspended in acetonitril (3 ml/mmol). To this suspension was added potassium carbonate (2 eq), t-butyl 4-bromobutanoate (1.1 eq) and potassium iodide (1.1 eq). The mixture was heated at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo and the residue was dissolved in EtOAc, washed with 5% NaHCO3 (15 ml/mmol). The organic layer was dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to yield 23d.
e)) Introduction of the 3-Cyclobutanecarboxylic Acid.
Compound 27 was suspended in 1,2-dichloroethane (20 ml/mmol). To this suspension was added 3-oxocyclobutanecarboxylic acid (1.3 eq) and sodium triacetoxy borohydride (1.6 eq). The mixture was stirred at room temperature for 16 hrs. If the reaction was not complete, another portion of 3-oxocyclobutanecarboxylic acid (1.3 eq) and sodium triacetoxy borohydride (1.6 eq) was added. After complete reaction, the solution was diluted with 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish pure compound 2e.
General Procedure for the Hydrogenation of 23 to Compound 22.
To a solution of compound 23 in ethanol (10 ml/mmol) was added palladium hydroxide on carbon (20%, 0.22 eg). Hydrogenation was started under atmospheric pressure of hydrogen. Stirring was continued for 16 hrs at room temperature. The mixture was filtered over Hyflo and the residue washed with ethanol. The filtrate was concentrated in vacuo to give compound 22.
General Procedure for the Synthesis of Compounds 28.
To a suspension of compound 20 in DCM (6 ml/mmol) were added DIPEA (1 eq), dimethylamino pyridine (DMAP, 0.05 eq) and di-t-butyl dicarbonate (1.1 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 28 in yields varying from 70-90%.
General Procedure for the Synthesis of Compounds 29.
Compound 28 was dissolved in 1,4-dioxan/water, 1/1, v/v (10 ml/mmol) and to the solution was added potassium hydroxide (4 eq, 11.7N) and the solution was degassed. To the solution was added 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.04 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.02 eq). The mixture was stirred at 80° C. for 16 hrs. The mixture was cooled to room temperature, diluted with EtOAc, acidified to pH 6 with 0.1N HCl and extracted with EtOAc. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 29 in yields varying from 60-95%.
General Procedure for the Synthesis of the Benzyl Ether Derivatives 30.
Method A) Compound 29 was dissolved in DCM/water, 2/1, v/v (4 ml/mmol) and to this solution was added sodium hydroxide (2N, 3 eq). To this mixture was added tetrabutylammonium bromide (0.1 eq) and the properly substituted benzyl bromide (1.1 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with DCM (15 ml/mmol), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 30 in yields varying from 80-90%.
Method B) Compound 29 was dissolved in N,N′-dimethylacetamide (4 ml/mmol) and to this solution was added triphenylphosphine (1.25 eq), diisopropyl azodicarboxylate (1.25 eq) and a properly substituted benzyl alcohol (1.2 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis showed complete reaction. The mixture was diluted with diethyl ether and washed with water (3×). The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 30 in yields varying from 70-90%.
Method C) Compound 28 was dissolved in toluene (8 mml/mmol) and to the solution was added potassium hydroxide (2 eq, 11.7N) and the solution was degassed. To the solution was added properly substituted benzyl bromide (1.1 eq), 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.03 eq). The mixture was stirred at 60° C. for 1.25 hrs. The mixture was cooled to room temperature, diluted with EtOAc and washed with 5% NaHCO3 solution (10 ml/mmol). The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography to give compound pure 30 in yields varying from 30-80%.
General Procedure for the Deprotection of Compounds 30 to 27.
Compound 30 was dissolved in DCM (10 ml/mmol) and trifluoroacetic acid (6 eq) was added. The solution was refluxed for 16 hrs after which time TLC analysis showed complete reaction. The mixture was neutralized with 5% aq. NaHCO3. The mixture was extracted with DCM (3×) and the combined organic layers were washed with brine, dried on Na2SO4 and concentrated in vacuo to give compound 27 which was used in the next step without further purification.
§5. Syntheses of Specific Compounds
(See Table 1)
All furanyl derivatives from Table 1 could be prepared by following either route A or B appropriate reagents. The following compounds are typical examples.
All oxazolo derivatives from Table 1 could be prepared by following either route C, D or E by choosing the appropriate reagents. The following compounds are typical examples.
3-[2-(4-Bromo-phenyl)-2-oxo-ethyl]-4-oxo-piperidine-1-carboxylic acid t-butyl ester (5, Rb=H)
To a solution of 4-oxo-piperidine-1-carboxylic acid t-butyl ester (4, 104.1 g, 522 mmol) in toluene (800 ml) was added a catalytic amount of para-toluenesulphonic acid mono hydrate (0.5 g, 2.6 mmol) and pyrrolidine (172.8 ml, 2090 mmol). The mixture was heated to reflux under Dean Stark conditions for 18 hours. The mixture was concentrated under reduced pressure and the residue was redissolved in toluene (600 ml). To this solution was added slowly (in minutes) a solution of 2-bromo-1-(4-bromo-phenyl)-ethanone (3, Rb=H, 145.2 g, 522 mmol) in toluene/DCM (900 ml, 1/2, v/v). The mixture was stirred overnight at room temperature and the resulting white slurry was poured out in water (1.5 L). The water layer was extracted with DCM (3×300 ml) and the combined organic layers were dried (MgSO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography (diethyl ether/petroleum ether, 2/3, v/v to 100% diethyl ether) giving compound 5 (Rb=H, 166.6 g, 87%) as a yellow solid. TLC analysis, Rf 0.3 in diethyl ether/petroleum ether, 1/1, v/v.
2-[4-Bromo-phenyl]-4,5,6,7-tetrahydro-furo[3,2-c]pyridine (6, Rb=H)
Compound 5 (Rb=H, 166 g, 456 mmol) was suspended in concentrated hydrochloric acid (500 ml, 12N, 6 mol). The mixture was heated with 10° C. per 30 minutes to 80° C. The mixture starts to foam heavily, so allow enough volume in the starting reaction vessel. After 45 minutes, the mixture was cooled to 0° C. and neutralized with 50 wt % solution of NaOH (exothermic). After stirring overnight at room temperature, the resulting solid material was collected by filtration and washed with 250 ml 0.1M NaOH. The light brown material was purified by Soxhlet extraction in EtOAc giving 6 (Rb=H, 51 g, 38%) as a beige solid which was used in the next step without further purification. LC-MS (Method A): Rt 1.19, [M+H] 278.
3-[2-(4-Bromo-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl]-propionic acid t-butyl ester (7a, Rb=H)
Compound 6 (Rb=H, 1.46 g, 5 mmol) was suspended in methanol (30 ml) and DIPEA was added (0.91 ml, 1.05 eg). To the mixture was added t-butyl acrylate (0.88 ml, 1.2 eq) and the mixture was refluxed for 16 hrs. Conversion was checked by TLC analysis (diethyl ether/petroleum ether, 1/1, v/v). The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 2/3 to 1/1, v/v) to give pure 7a (Rb=H, 1.75 g, 86%) as a white solid. LC-MS (Method A): Rt 1.38, [M+H] 407.
3-[2-(4-Hydroxy-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl]-propionic acid t-butyl ester (8a, Rb=H)
Compound 7a (Rb=H, 3.85 g, 9.5 mmol) was dissolved in toluene (80 ml) and to the solution was added potassium hydroxide (2 eq, 11.7N) and the solution was degassed. To the solution was added 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.24 g, 0.57 mmol, 0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.26 g, 0.28 mmol, 0.03 eq). The mixture was stirred at 60° C. for 1.25 hrs. The mixture was cooled to room temperature, diluted with EtOAc and washed with 5% NaHCO3 solution (10 ml/mmol). The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 1/1, v/v, Rf 0.1) to give pure compound 8a (Rb=H, 1.86 g, 57%) as a yellow solid. LC-MS (Method A): Rt 1.14, [M+H] 344.
3-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-propionic acid t-butyl ester (9a, Ra=2F, Rb=H)
Compound 8a (Rb=H, 1.24 g, 3.61 mmol) was dissolved in N,N-dimethylacetamide (10 ml) and to this solution was added triphenylphosphine (1.33 g, 5.06 mmol, 1.4 eq), diisopropyl azodicarboxylate (1 ml, 5.05 mmol, 1.4 eq) and 2-fluorobenzyl alcohol (0.46 ml, 4.33 mmol, 1.2 eq). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis (diethyl ether, Rf 0.3) showed complete reaction. The mixture was diluted with diethyl ether and washed with water (3×). The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 1/1, v/v to 2/1, v/v) to give compound pure 9a (Ra=2F, Rb=H, 1.38 g, 84%) as an oil. LC-MS (Method A): Rt 1.46, [M+H] 452.
3-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-propionic acid (33)
Compound 9a (Ra=2F, Rb=H, 1.38 g, 3.1 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 30 ml) and the mixture was stirred for 2 hrs at 35° C. The solvents were evaporated and diisopropyl ether (30 ml) was added to precipitate the product as the hydrochloric acid salt. The white solid material was filtered and dried in vacuo to give compound 33 (0.75 g, 54%) as a white solid. 1H NMR (400 MHz, DMSO-d6): δ ppm 2.93 (t, J=7.6 Hz, 2H), 3.07 (bs, 2H), 3.28-3.55 (bs, 2H), 3.44 (t, J=7.6 Hz, 2H), 3.60-3.90 (bs, 2H), 4.06-4.56 (bs, 2H), 5.17 (s, 2H), 6.76 (s, 1H), 7.10 (d, J=8.8 Hz, 2H), 7.21-7.31 (m, 2H), 7.39-7.48 (m, 1H), 7.57 (t, J=7.5 Hz, 1H), 7.63 (d, J=8.8 Hz, 2H), 10.7-11.5 (bs, 1H), 12.3-13.2 (bs, 1H); LC-MS (Method A): Rt 1.39, [M+H] 396.
2-(4-Bromo-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-carboxylic acid t-butyl ester 10 (Rb=H)
To a suspension of compound 6 (Rb=H, 5 g, 17 mmol) in DCM (100 ml) were added DIPEA (2.92 ml, 1 eq), DMAP (0.1 g, 0.05 eq) and di-t-butyl dicarbonate (4.1 g, 18.8 mmol, 1.1 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis (DCM, Rf, 0.40) revealed complete reaction. The reaction mixture was washed with 5% ag. NaHCO3 solution and the resulting water layers were extracted with DCM. The combined organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (eluent: 100% DCM) to give compound 10 (Rb=H, 5.99 g, 92%) as an oil.
2-(4-Hydroxy-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-carboxylic acid t-butyl ester 11a (Rb=H)
Compound 10 (Rb=H, 11.77 g, 31 mmol) was dissolved in 1,4-dioxan/water, 1/1, v/v (200 ml) and to the solution was added potassium hydroxide (6.98 g, 124.5 mmol, 4 eq) and the solution was degassed. To the solution was added 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.53 g, 1.24 mmol, 0.04 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.57 g, 0.62 mmol, 0.02 eq). The mixture was stirred at 80° C. for 16 hrs. The mixture was cooled to room temperature, diluted with EtOAc, acidified to pH 6 with 0.1N HCl and extracted with EtOAc. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (eluent: DCM/MeOH, 1/0 to 99.5/0.5) to give compound 11 (Rb=H, 9 g, 90%) as a white solid.
2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-carboxylic acid t-butyl ester (12, Ra=4Cl, Rb=H)
Compound 11a (Rb=H, 2.0 g, 6.34 mmol) was dissolved in DCM/water, 2/1, v/v (30 ml) and to this solution was added sodium hydroxide (2N, 10 ml). To this mixture was added tetrabutylammonium bromide (0.2 g, 0.63 mmol, 0.1 eq) and 4-chlorobenzyl bromide (1.43 g, 6.98 mmol, 1.1 eg). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis (100% DCM, Rf 0.55) showed complete reaction. The mixture was diluted with DCM (15 ml/mmol), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (DCM/petroleum ether, 3/1 to 1/0, v/v) to give compound 12 (Ra=4Cl, Rb=H, 2.3 g, 82%) as a yellow oil. 1H NMR (400 MHz, CDCl3) δ ppm 1.4 (s, 9H); 2.75 (bs, 2H); 3.75 (bs, 2H); 4.35 (bs, 2H); 5.05 (s, 2H); 6.4 (s, 1H), 6.94 (d, 1H); 7.30-755 (m, 7H).
2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine (13, Ra=4Cl, Rb=H)
Compound 12 (Ra=4Cl, Rb=H, 2.3 g, 5.2 mmol) was dissolved in DCM (50 ml) and trifluoroacetic acid (4 ml, 10 eq) was added. The solution was refluxed for 16 hrs after which time TLC analysis (100% DCM, Rf 0.05) showed complete reaction. The mixture was neutralized with 5% aq. NaHCO3. The mixture was extracted with DCM (3×) and the combined organic layers were washed with brine, dried on Na2SO4 and concentrated in vacuo to give crude 13 (Ra=4Cl, Rb=H, 1.79 g) which was used in the next step without further purification. LC-MS (Method A): Rt 1.49, [M+H] 340.
3-{2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-2-methyl-propionic acid t-butyl ester (9b, Ra=4Cl, Rb=H)
To a solution of compound 13a (0.25 g, 0.74 mmol) in DMF (5 ml) in a 25 ml pyrex bottle were added 1,8-diazabicyclo[5.4.0]undec-7-ene (0.33 ml, 2.21 mmol) and t-butylmethacrylate (0.24 ml, 1.47 mmol). The mixture was heated at 140° C. for 16 hrs. The solution was cooled and 5% NaHCO3 was added (10 ml) and extracted with diethyl ether/EtOAc, 1/1, v/v. The organic layer was washed with water (4×20 ml), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 9/1 to 4/1, v/v, Rf 0.65) to give pure 9b (Ra=4Cl, Rb=H, 0.1 g, 28%) as a colorless oil. LC-MS (Method A): Rt 1.88, [M+H] 482.
3-{2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-2-methyl-propionic acid (77)
Compound 9b (Ra=4Cl, Rb=H, 0.12 g, 0.25 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 2.8 ml) and the mixture was stirred for 16 hrs at room temperature. The solvent was evaporated and diisopropyl ether (30 ml) was added to precipitate the product as the hydrochloric acid salt. The white solid material was filtered and dried in vacuo to give compound 77 (0.09 g, 74%) as a white solid. 1H NMR (400 MHz, DMSO-d6): δ ppm 1.29 (d, J=7.2 Hz, 3H), 3.05-3.17 (m, 3H), 3.23 (dd, J=5.4, 13.3 Hz, 1H), 3.51-3.68 (m, 3H), 4.24 (br. s., 2H), 5.16 (s, 2H), 6.73 (s, 1H), 7.08 (d, J=8.9 Hz, 2H), 7.42-7.53 (m, 4H), 7.61 (d, J=8.9 Hz, 2H), 10.4-13.1 (bs, 2H); 13C NMR (101 MHz, DMSO-d6): δ ppm 16.42 (q, 1C), 20.36 (t, 1C), 35.17 (d, 1C), 48.74 (t, 1C), 49.57 (t, 1C), 56.96 (t, 1C), 68.60 (t, 1C), 102.92 (d, 1C), 113.06 (s, 1C), 115.41 (d, 1C), 123.20 (s, 1C), 124.87 (d, 1C), 128.41 (d, 1C), 129.42 (d, 1C), 132.46 (s, 1C), 136.01 (s, 1C), 145.10 (s, 1C), 152.99 (s, 1C), 157.85 (s, 1C), 174.95 (s, 1C). LC-MS (Method A): Rt 1.56, [M+H] 426.
2-[4-(Benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-carboxylic acid t-butyl ester 12 (Ra=Rb=H)
Compound 11 (Rb=H, 0.84 g, 2.66 mmol) was dissolved in DCM/water, 2/1, v/v (30 ml) and to this solution was added sodium hydroxide (2N, 4.2 ml). To this mixture was added tetrabutyl-ammonium bromide (0.09 g, 0.27 mmol, 0.1 eq) and benzyl bromide (0.35 ml, 2.93 mmol, 1.1 eg). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis (DCM/MeOH, 98/2, v/v, Rf 0.8) showed complete reaction. The mixture was diluted with DCM (100 ml), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (DCM/petroleum ether, 3/1 to 1/0, v/v) to give compound 12 (Ra=Rb=H, 1.03 g, 95%) as a white solid. 1H NMR (400 MHz, CDCl3) δ ppm 1.4 (s, 9H); 2.75 (bs, 2H); 3.75 (bs, 2H); 4.35 (bs, 2H); 5.05 (s, 2H); 6.35 (s, 1H); 6.98 (d, 2H); 7.30-7.55 (m, 7H).
2-[4-Benzyloxy-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine (13, Ra=Rb=H)
Compound 12 (Ra=Rb=H, 1.03 g, 2.54 mmol) was dissolved in DCM (20 ml) and trifluoroacetic acid (1.5 ml) was added. The solution was refluxed for 16 hrs after which time TLC analysis (100% DCM, Rf 0.05) showed complete reaction. The mixture was neutralized with 5% aq. NaHCO3 (40 ml) and extracted with DCM (3×50 ml) and the combined organic layers were washed with brine, dried on Na2SO4 and concentrated in vacuo to give compound 13 (Ra=Rb=H, 0.67 g, 86%) which was used in the next step without further purification. LC-MS (Method A): Rt 1.50, [M+H] 306.
3-{2-[4-Benzyloxy-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid t-butyl ester (9c, Ra=Rb=H)
Compound 13 (Ra=Rb=H, 0.16 g, 0.52 mmol) was suspended in 1,2-dichloroethane (3.2 ml). To this suspension was added t-butylacetoacetate (0.09 ml, 0.52 mmol) and sodium triacetoxy borohydride (0.16 g, 0.73 mmol). The mixture was stirred at room temperature for 16 hrs, after which time another portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) were added together with a drop of acetic acid. After stirring for another 60 hrs, again a portion of t-butylacetoacetate (1 eq) and sodium triacetoxy borohydride (1.4 eq) were added and stirring was continued for 36 hrs. The solution was diluted with 5% NaHCO3 (10 ml) and the mixture was extracted with DCM (3×100 ml). The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 9/1 to 4/1, v/v) to furnish pure compound 9c (Ra=Rb=H, 0.06 g, 25%) as a white solid. LC-MS (Method A): Rt 1.68, [M+H] 448.
3-{2-[4-Benzyloxy-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid (76)
Compound 9c (Ra=Rb=H, 0.08 g, 0.18 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4 ml, 2N) and the mixture was stirred for 16 hrs at room temperature. The solvents were evaporated and the residue was co-evaporated with cyclohexane. Diisopropyl ether (30 ml) was added to precipitate the product as the hydrochloric acid salt, the white solid material was filtered and dried in vacuo to give compound 76 (0.06 g, 80%). 1H NMR (400 MHz, DMSO-d6), δ ppm: 1.38 (d, J=6.6 Hz, 3H), 2.58-2.75 (m, 1H), 2.90-3.18 (m, 3H), 3.39-3.54 (m, 1H), 3.61-3.78 (m, 1H), 3.80-3.93 (m, 1H), 4.13-4.32 (m, 2H), 5.14 (s, 2H), 6.76 (s, 1H), 7.07 (d, J=8.8 Hz, 2H), 7.30-7.36 (m, 1H), 7.40 (s, 2H), 7.45 (s, 2H), 7.62 (d, J=8.8 Hz, 2H), 10.15-10.80 (m, 1H), 12.54-13.10 (m, 1H). LC-MS (Method A): Rt 1.46, [M+H] 392.
4-[2-(4-Bromo-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl]-butyric acid t-butyl ester (7d)
Compound 6 (Rb=H, 4.55 g, 16.3 mmol) was suspended in acetonitril (55 ml). To this suspension were added potassium carbonate (4.52 g, 32.7 mmol), t-butyl 4-bromobutanoate (4.38 g, 19.6 mmol, 1.2 eq) and potassium iodide (3.2 g, 19.6 mmol, 1.2 eq). The mixture was heated to reflux for 16 hrs after which time TLC analysis (diethyl ether/petroleum ether, 1/1, v/v, Rf 0.1) revealed complete reaction. The mixture was concentrated in vacuo and the residue was dissolved in EtOAc and washed with 5% NaHCO3 (2×60 ml). The organic layer was dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 1/1, v/v) to yield 7d (Rb=H, 4.94 g, 71%) as a yellow solid. LC-MS (Method A): Rt 1.37, [M+H] 420.
4-[2-(4-Hydroxy-phenyl)-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl]-butyric acid t-butyl ester (8d)
Compound 7d (Rb=H, 4.91 g, 11.68 mmol) was dissolved in toluene (100 ml) and to the solution was added potassium hydroxide (2 ml, 11.7N) and the solution was degassed. To the solution was added 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (0.27 g, 0.64 mmol, 0.06 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (0.29 g, 0.32 mmol, 0.03 eq). The mixture was stirred at 60° C. for 1.25 hrs. The mixture was cooled to room temperature, diluted with EtOAc and washed with 5% NaHCO3 solution (100 ml). The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 1/1 to 1/0, v/v, Rf 0.1) to give pure compound 8d (Rb=H, 4.0 g) as a yellow solid. LC-MS (Method A): Rt 1.21, [M+H] 358.
4-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid t-butyl ester (9d)
Compound 8d (Rb=H, 0.43 g, 1.2 mmol) was dissolved in DCM/water, 2/1, v/v (5 ml) and to this solution was added sodium hydroxide (1.8 ml, 2N, 3 eq). To this mixture was added tetrabutylammonium bromide (0.1 eq) and 2F-benzyl bromide (1.32 mmol, 250 mg). The mixture was stirred for 16 hrs at room temperature after which time TLC analysis (diethyl ether, Rf 0.5) showed complete reaction. The mixture was diluted with DCM (15 ml), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 1/1 to 1/0, v/v to give compound 9d (Ra=2F, Rb=H) in a yield of 80%. LC-MS (Method A): Rt 1.49, [M+H] 466.
4-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid (35)
Compound 9d (Ra=2F, Rb=H, 0.3 g, 0.64 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 2.8 ml) and the mixture was stirred for 16 hrs at room temperature. The solvents were evaporated and diisopropyl ether (30 ml) was added to precipitate the product as the hydrochloric acid salt. The white solid material was filtered and dried in vacuo to give compound 35 (0.32 g, 95%) as a white solid. 1H NMR (400 MHz, DMSO-d6) □ PPM: 1.69-1.83 (m, 2H), 2.18 (t, J=7.2 Hz, 2H), 2.81-2.91 (m, 2H), 3.02-3.13 (m, 2H), 3.23-3.36 (bs, 1H), 3.50-3.68 (bs, 1H), 3.89-4.05 (m, 1H), 4.15-4.29 (m, 1H), 4.98 (s, 2H), 6.58 (s, 1H), 6.91 (d, J=8.7 Hz, 2H), 7.02-7.12 (m, 2H), 7.21-7.29 (m, 1H), 7.38 (dt, J=7.7, 1.5 Hz, 1H), 7.44 (d, J=8.7 Hz, 2H), 9.80 (br.s., 1H), 11.37-13.06 (bs, 1H); LC-MS (Method A): Rt 1.37, [M+H] 410.
4-{2-[4-(2-Fluoro-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-4H-furo[3,2-c]-pyri-dine-5-yl}-butyric acid (73)
Compound 73 was prepared in a similar fashion as described for 35 starting from 2-bromo-1-(4-bromo-2-fluoro-phenyl)-ethanone. Compound 73: 1H NMR (400 MHz, DMSO-d6), □ PPM: 1.92-2.04 (m, 2H), 2.38 (t, J=7.1 Hz, 2H), 3.02-3.13 (m, 2H), 3.17-3.28 (m, 2H), 3.44-3.92 (bs, 2H), 3.98-4.54 (bs, 2H), 5.21 (s, 2H), 6.70 (d, J=2.8 Hz, 1H), 7.00 (dd, J=8.9, 1.9 Hz, 1H), 7.12 (dd, J=13.4, 1.9 Hz, 1H) 7.23-7.32 (m, 2H) 7.41-7.50 (m, 1H) 7.56-7.63 (m, 1H) 7.69 (t, J=9.0 Hz, 1H), 10.06-10.93 (bs, 1H), 12.07-12.85 (bs, 1H); LC-MS (Method A): Rt 1.35, [M+H] 428.
2-(4-Bromo-phenyl)-oxazolo[4,5-c]pyridine (17, Rb=H)
To a cooled (0° C.) suspension of commercially available 4-hydroxy-3-amino-pyridine 14 (4 g, 36 mmol) in DCM (200 ml) were added triethyl amine (6.3 ml, 1.25 eq) and a solution of 4-bromo-benzoyl chloride (15, Rb=H, 8 g, 36 mmol, 1 eq, 0.3M in DCM). The reaction mixture was allowed to reach room temperature and the mixture was stirred for 16 hrs. The mixture was filtered, washed with DCM and ether to furnish crude 16 (Rb=H) as a solid material which was used in the next step without further purification. Hexachloroethane (10.2 g, 43 mmol, 2.5 eq) was dissolved in DCM (150 ml) and triphenyl phosphine (13.56 g, 51.69 mmol, 3 eq) and triethyl amine (19.2 ml, 137.8 mmol, 8 eq) was added. The mixture was stirred for minutes at room temperature and crude compound 16 (Rb=H) was added slowly in 5 equal portions. The mixture was stirred at room temperature for 64 hrs after which time TLC analysis (DCM/MeOH, 97/3, v/v, Rf 0.3) revealed complete reaction. The solution was concentrated and the residue was suspended in DCM. The mixture was filtered and the residue washed with DCM and diethyl ether to give crude 17 (Rb=H) which was used in the next step without further purification.
2-(4-Bromo-phenyl)-5-methyl-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine (19, Rb=H)
To a solution of compound 17 (Rb=H, 11.4 mmol) in DMF (95 ml) was added iodomethane (2.84 ml, 45.58 mmol, 4 eg) and the mixture was stirred for 16 hrs. The mixture was concentrated in vacuo and the residue was stirred in EtOAc to give crude 18 (Rb=H, 3.3 g, 69%) as a white solid. Compound 18 (Rb=H, 2.3 g, 5.5 mmol) was dissolved in methanol (55 ml) and the solution was cooled to 0° C. Sodium borohydride (0.42 g, 11 mmol, 2 eg) was added and the mixture was stirred at 0° C. for 2 hrs after which time it was allowed to reach room temperature and stirring was continued for 16 hrs. Water was added (4 ml) and the mixture was stirred for 5 minutes. The mixture was co-evaporated with acetonitril and the residue was purified by silica gel column chromatography (DMA 0.5) to yield compound 19 (Rb=H) in a yield of 61%.
2-(4-Bromo-phenyl)-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine (20, Rb=H)
To a cooled (0° C.) solution of compound 19 (Rb=H, 0.95 g, 3.2 mmol) in 1,2-dichloroethane (32 ml) was added DIPEA (1.1 ml, 6.4 mmol, 2 eq). At 0° C., 1-chloroethylchloroformate (1.05 ml, 9.72 mmol, 3 eq) was added and the mixture was stirred for 10 minutes at 0° C. after which time the temperature was raised to reflux temperature. After 2 hrs, the mixture was concentrated in vacuo and the residue was dissolved in methanol (35 ml). The solution was stirred for 48 hrs at room temperature. The precipitate was filtered, the solid product was washed with diethyl ether to give compound 20 (Rb=H, 0.9 g, 88%). LC-MS (Method A): Rt 1.1, [M+H] 280.
3-[2-(4-Bromo-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl]-propionic acid t-butyl ester (21, Rb=H)
Compound 20 (Rb=H, 8 g, 22.8 mmol) was suspended in methanol (200 ml) and DIPEA was added (8.15 ml, 46.8 mmol, 2.05 eg). To the mixture was added t-butyl acrylate (3.97 ml, 27.4 mmol, 1.2 eq) and the mixture was refluxed for 120 rs. Conversion was checked by TLC analysis and after 16 and 64 hrs additional t-butyl acrylate (3.97 ml, 27.4 mmol, 1.2 eq) was added to push the reaction to completion. The solvents were evaporated and the residue was redissolved in EtOAc and extracted with a 5% solution of NaHCO3. The organic layer was dried (MgSO4), concentrated in vacuo and the residue was purified by silica gel column chromatography (eluent: diethyl ether/petroleum ether, 1/1, v/v) to give 21a (Rb=H, 8.8 g, 93%). LC-MS (Method A): Rt 1.38, [M+H] 408.
3-{2-[4-(Benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-propionic acid t-butyl ester (23a, Ra=Rb=H)
Compound 21a (Rb=H, 0.9 g, 2.21 mmol) was dissolved in degassed toluene (7 ml) and to the solution was added cesium carbonate (1.08 g, 3.3 mmol), benzyl alcohol (0.46 ml, 4.42 mmol, 2 eq), 2-di-t-butylphosphino-3,4,5,6-tetramethyl-2′,4′,6′-triisopropyl-1,1-biphenyl (25.5 mg, 0.05 mmol, 0.02 eq) and palladium(II) acetate (9.92 mg, 0.04 mmol, 0.02 eq). The mixture was stirred at 70° C. for 16 hrs. The mixture was cooled to room temperature, concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 3/1, v/v) to give pure 23a (Ra=Rb=H, 0.71 g, 74%) as a white solid. LC-MS (Method A): Rt 1.46, [M+H] 435.
3-{2-[4-(Benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-propionic acid (47)
Compound 23a (Ra=Rb=H, 0.71 g, 1.63 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 12 ml, 30 eq) and the mixture was stirred for 16 hrs at 50° C. The solvents were evaporated and diethyl ether was added to precipitate the product. The white solid material was filtered and dried in vacuo to give compound 47 (0.67 g, 93%) as a white solid. 1H NMR (400 MHz, DMSO-d6): □ ppm, 2.92 (t, J=7.1 Hz, 2H), 3.06-3.31 (bs, 2H), 3.57 (t, J=7.1 Hz, 2H), 3.50-3.61 (bs, 1H), 3.79-4.04 (bs, 1H), 4.21-4.42 (bs, 1H), 4.42-4.60 (bs, 1H), 5.20 (s, 2H), 7.16 (d, J=8.6 Hz, 2H), 7.35 (t, J=7.5 Hz, 1H), 7.41 (t, J=7.5 Hz, 2H), 7.49 (d, J=7.5 Hz, 2H), 7.93 (d, J=8.6 Hz, 2H), 10.31-10.84 (bs, 1H). LC-MS (Method A): Rt 1.32, [M+H] 379.
4-[2-(4-Bromo-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl]-butyric acid t-butyl ester (21d, Rb=H)
Compound 20 (Rb=H, 2.5 g, 7.92 mmol) was suspended in DMF (40 ml). To this suspension was added potassium carbonate (3.8 g, 27.7 mmol, 3.5 eq) and t-butyl 4-bromobutanoate (5.3 g, 23.7 mmol, 3 eq). The mixture was heated at 80° C. for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo and the residue was purified by silica gel column chromatography (eluent: diethyl ether/petroleum ether, 1/1, v/v to 100% diethyl ether) to yield 21d (Rb=H, 3.15 g, 94%) as a white solid.
4-{2-[4-(2,3-Difluoro-benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid t-butyl ester (23d, Ra=2,3-diF, Rb=H)
Compound 21d (Rb=H, 0.6 g, 1.42 mmol) was dissolved in degassed toluene (5 ml) and to the solution was added cesium carbonate (0.7 g, 2.14 mmol), 2,3-difluoro-benzyl alcohol (0.32 ml, 2.85 mmol, 2 eq), 2-di-t-butylphosphino-3,4,5,6-tetramethyl-2′,4′,6′-triisopropyl-1,1-biphenyl (16.43 mg, 0.03 mmol, 0.02 eq) and palladium(II) acetate (6.39 mg, 0.03 mmol, 0.02 eq). The mixture was stirred at 70° C. for 16 hrs. The mixture was cooled to room temperature, concentrated in vacuo and the residue was purified by silica gel column chromatography (diethyl ether/petroleum ether, 2/1, v/v to 100% diethyl ether) to give pure 23d (Ra=2,3-diF, Rb=H, 0.48 g, 70%) as an oil.
4-{2-[4-(2,3-Difluoro-benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (57)
Compound 23d (Ra=2,3-diF, Rb=H, 0.46 g, 0.95 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 14 ml, 60 eq) and the mixture was stirred for 16 hrs at room temperature. The solvents were evaporated and diethyl ether was added to precipitate the product. The white solid material was filtered and dried in vacuo to give compound 57 (0.45 g, 99%) as a white solid. 1H NMR (400 MHz, DMSO-d6): □□ ppm 2.08 (m., 2H), 2.41 (t, J=7.0 Hz, 2H), 3.00-3.29 (m, 2H), 3.31-3.40 (m, 2H), 3.48-3.70 (bs, 1H), 3.70-3.96 (bs, 1H), 4.18-4.38 (m, 1H), 4.38-4.61 (m, 1H), 5.26 (s, 2H), 7.16 (d, J=8.8 Hz, 2H), 7.19-7.25 (m, 1H), 7.28-7.39 (m, 2H), 7.94 (d, J=8.8 Hz, 2H), 10.37-10.88 (bs, 1H); LC-MS (Method A): Rt 1.16, [M+H] 429.
4-{2-[4-Benzyloxy-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (53)
Compound 53 was prepared in a similar fashion as described for the synthesis of 57 starting from 23d (Ra=Rb=H). 1H NMR (400 MHz, DMSO-d6): □ ppm 2.01-2.17 (m, 2H), 2.42 (t, J=6.9 Hz 2H), 3.00-3.30 (m, 2H), 3.32-3.41 (m, 2H), 3.50-3.69 (m, 1H), 3.78-3.96 (m, 1H), 4.18-4.37 (m, 1H), 4.43-4.59 (m, 1H), 5.18 (s, 2H), 7.14 (d, J=8.8 Hz, 2H), 7.34 (t, J=7.9 Hz, 1H), 7.40 (t, J=7.9 Hz, 2H), 7.47 (d, J=7.9 Hz, 2H), 7.93 (d, J=8.8 Hz, 2H), 10.45-10.89 (bs, 1H); LC-MS (Method A): Rt 1.17, [M+H] 393.
4-{2-[4-(4-Trifluoromethyl-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (85)
Compound 85 was prepared following route C starting from 2-fluoro-4-bromo-benzoyl chloride. 1H NMR (400 MHz, DMSO-d6), □ ppm: 1.98-2.11 (m, 2H), 2.40 (t, J=7.1 Hz, 2H), 3.10-3.24 (m, 2H), 3.30-3.42 (m, 2H), 3.51-3.66 (bs, 1H), 3.79-3.97 (bs, 1H), 4.25-4.39 (m, 1H), 4.49-4.65 (m, 1H), 5.32 (s, 2H), 6.98-7.13 (m, 2H), 7.66-7.78 (m, 4H), 7.93 (t, J=8.6 Hz, 1H), 9.91-10.46 (bs, 1H); LC-MS (Method A): Rt 1.77, [M+H] 479.
4-{2-[4-(2-Fluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (89)
Compound 89 was prepared following route C starting from 2-methyl-4-bromo-benzoyl chloride. 1H NMR (400 MHz, DMSO-d6), □ ppm: 2.00-2.12 (m, 2H), 2.41 (t, J=7.1 Hz, 2H), 2.64 (s, 3H), 3.05-3.26 (m, 2H), 3.32-3.42 (m, 2H), 3.50-3.70 (bs, 1H), 3.79-3.95 (bs, 1H), 4.22-4.39 (m, 1H), 4.48-4.65 (m, 1H), 5.21 (s, 2H), 6.97-7.04 (m, 2H), 7.17-7.27 (m, 2H), 7.37-7.46 (m, 1H), 7.56 (dt, J=7.4, 1.4 Hz, 1H), 7.89 (d, J=8.5 Hz, 1H), 9.97-10.46 (bs, 1H); LC-MS (Method A): Rt 1.61, [M+H] 425.
4-{2-[4-(3,4-Dichloro-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (227)
Compound 227 was prepared following route C starting from 2-fluoro-4-bromo-benzoyl chloride. 1H NMR (600 MHz, DMSO-d6), □ ppm: 1.74 (q, J=7.1 Hz, 2H), 2.26 (t, J=7.1 Hz, 2H), 2.55 (t, J=7.1 Hz, 2H), 2.73-2.78 (m, 2H), 2.81 (t, J=5.3 Hz, 2H), 3.43 (br.s, 2H), 5.21 (s, 2H), 7.00 (dd, J=8.8, 2.5 Hz, 1H), 7.11 (dd, J=13.0, 2.5 Hz, 1H), 7.47 (dd, J=8.4, 2.0 Hz, 1H), 7.69 (d, J=8.3 Hz, 1H), 7.76 (d, J=1.9 Hz, 1H), 7.89 (t, J=8.3 Hz, 1H), 11.30-12.80 (bs, 1H); LC-MS (Method A): Rt 1.42, [M+H] 479.
4-{2-[4-(2-Fluoro-benzyloxy)-3-chloro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (283)
Compound 283 was prepared following route C starting from 3-chloro-4-bromo-benzoyl chloride. 1H NMR (400 MHz, DMSO-d6): □ ppm 1.69-1.801.74 (m, 2H), 2.27 (t, J=7.2 Hz, 2H), 2.55 (t, J=7.0 Hz, 2H), 2.73-2.79 (m, 2H), 2.81 (t, J=4.6 Hz, 2H), 3.43 (s, 2H), 5.32 (s, 2H), 7.25-7.33 (m, 2H), 7.42-7.50 (m, 2H), 7.58-7.65 (m, 1H), 7.88 (dd, J=8.7, 2.1 Hz, 1H), 7.93 (d, J=2.1 Hz, 1H), 11.0-13.0 (bs, 1H); LC-MS (Method B): Rt 1.99*, [M+H] 445.
4-{2-[4-(4-Chloro-benzyloxy)-3-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (211)
Compound 211 was prepared following route C starting from 3-fluoro-4-bromo-benzoyl chloride. 1H NMR (400 MHz, DMSO-d6) □ ppm: 1.95-2.06 (m, 2H), 2.38 (t, J=7.2 Hz, 2H), 3.09-3.20 (m, 2H), 3.23-3.32 (m., 2H), 3.44-3.53 (m, 1H), 3.76-3.86 (m, 1H), 4.20-4.32 (m, 1H), 4.43-4.52 (m, 1H), 5.28 (s, 2H), 7.43 (t, J=8.6 Hz, 1H), 7.51 (m, 4H), 7.76 (m, 2H), 10.65-11.02 (bs, 1H), 12.03-12.77 (bs, 1H); LC-MS (Method B): Rt 2.03*, [M+H] 445.
2-(4-Benzyloxy-phenyl)-oxazolo[4,5-c]pyridine (25, Ra=Rb=H)
To a cooled (0° C.) suspension of commercially available 4-hydroxy-3-amino-pyridine (14, 19.3 g, 175 mmol) in acetonitril (1500 ml) was added 4-benzyloxy-benzoic acid (24, Ra=Rb=H, 40 g, 175 mmol), triphenylphosphine (142.5 g, 543 mmol, 3.1 eq) and trichloroactonitril (54.5 ml, 543 mmol, 3.1 eq). The reaction mixture was allowed to reach room temperature and the mixture was stirred for 16 hrs at 80° C. The mixture was concentrated in vacuo and the residues was dissolved in DCM and washed with 2N NaOH (3×). The combined water layers were extracted with DCM and the organic layers dried (Na2SO4) to give crude 25 (Ra=Rb=H) as oil which was used in the next step without further purification.
2-(4-Benzyloxy-phenyl)-5-methyl-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine (26, Ra=Rb=H)
To a solution of crude 25 (Ra=Rb=H, 117 mmol) in DMF (540 ml) was added iodomethane (29.35 ml, 471 mmol, 4 eg) and the mixture was stirred for 16 hrs. The mixture was concentrated in vacuo and the residue was stirred in EtOAc to give the quaternary salt of 25 a white solid, which dissolved in methanol (950 ml) and the solution was cooled to 0° C. Sodium borohydride (10.2 g, 268 mmol, 2.5 eg) was added and the mixture was stirred at 0° C. for 2 hrs after which time it was allowed to reach room temperature and stirring was continued for 64 hrs. Water was added (117 ml) and the mixture was stirred for 5 minutes. The mixture was concentrated in vacuo, the residues suspended in 2N NaOH (5 ml/mmol) and extracted with DCM (3×). The combined organic layers were dried (Na2SO4) and concentrated to give crude 26 (Ra=Rb=H) as a yellow solid which was used in the next step without further purification.
2-(4-Benzyloxy-phenyl)-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine (27, Ra=Rb=H)
To a cooled (0° C.) solution of compound 26 (Ra=Rb=H, 35.2 g, 109.8 mmol) in 1,2-dichloroethane (880 ml) was added DIPEA (37.61 ml, 219.7 mmol, 2 eq) and 1-chloroethyl chloroformate (35.56 ml, 329.6 mmol, 3 eq) was added. The mixture was stirred for 10 minutes at 0° C. after which time the temperature was raised to reflux temperature. After 4 hrs, the mixture was allowed to reach room temperature and stirring was continued for 16 hrs. The mixture was concentrated in vacuo and the residue was dissolved in methanol (880 ml). The solution was stirred for 16 hrs at room temperature after which time TLC analysis revealed the reaction to be complete. Removal of the solvent resulted in the isolation of crude 27 (Ra=Rb=H) in an overall yield of 20% based on 25 (Ra=Rb=H).
3-[2-(4-Benzyloxy-phenyl)-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine]-2-methyl-propionic acid t-butyl ester (28b)
To a solution of compound 27 (Ra=Rb=H, 13.45 g, 43.9 mmol) in DMF (270 ml) was added 1,8-diazabicyclo[5.4.0]undec-7-ene (3 eg) and t-butylmethacrylate (28.54 ml, 175.6 mmol, 4 eq). The mixture was heated at 125° C. for 100 hrs. The solution was cooled and 5% NaHCO3 was added (15 ml/mmol) and extracted with diethyl ether/EtOAc, 1/1, v/v. The organic layer was washed with water (4×), dried on MgSO4, concentrated in vacuo and the residue was purified by silica gel column chromatography (eluent: diethyl ether/petroleum ether, 1/2 to 3/1, v/v) to give 23b (Ra=Rb=H, 14.58 g, 74%) as an oil.
3-[2-(4-Benzyloxy-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl]-2-methyl-propionic acid (146)
Compound 23b (Ra=Rb=H, 0.45 g, 1 mmol) was dissolved in a solution of HCl in 1,4-dioxan (4N, 14 ml, 60 eq) and the mixture was stirred for 16 hrs at room temperature. The solvents were evaporated and diethyl ether was added to precipitate the product. The white solid material was filtered and dried in vacuo to give compound 146 (0.43 g, 99%) as a white solid. 1H NMR (400 MHz, DMSO-d6), □ ppm: 1.28 (d, J=7.3 Hz, 3H), 3.07-3.20 (m, 3H), 3.26 (dd, J=13.2, 4.9 Hz, 1H), 3.60 (dd, J=13.2, 7.9 Hz, 1H), 3.66 (bs, 2H), 4.34 (br. s., 2H), 5.18 (s, 2H), 7.11-7.17 (m, 2H), 7.31-7.37 (m, 1H), 7.37-7.43 (m, 2H), 7.44-7.49 (m, 2H), 7.85-7.93 (m, 2H), 10.17-12.85 (bs, 1H); LC-MS (Method A): Rt 1.48, [M+H] 393.
3-{2-[4-(4-Trifluoromethyl-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid (156)
Compound 156 was prepared following Route C. 1H NMR (400 MHz, DMSO-d6): □ ppm: 1.29 (d, J=7.2 Hz, 3H), 3.09-3.21 (bs, 3H), 3.27 (dd, J=13.1, 5.3 Hz, 1H), 3.57-3.72 (dd, J=13.1, 7.3 Hz, 1H), 3.65-3.72 (bs, 2H), 4.34 (br. s., 2H), 5.33 (s, 2H), 7.21 (d, J=8.8 Hz, 2H), 7.68-7.74 (m, 2H), 7.75-7.81 (m, 2H), 7.93 (d, J=8.8 Hz, 2H), 10.50-12.32 (bs, 1H); 13C NMR (101 MHz, DMSO-d6), □ PPM: 16.35 (q, 1C), 19.25 (t, 1C), 35.14 (d, 1C), 49.04 (br. t., 1C), 49.76 (br. t., 1C), 57.23 (t, 1C), 68.66 (t, 1C), 115.60 (d, 2C), 119.65 (s, 1C), 124.21 (s, 1JCF=272.5 Hz, 1C), 125.34 (d, 3JCF=3.6 Hz, 2C), 127.63 (d, 2C), 128.05 (d, 2C), 128.51 (s, 2JCF=31.7 Hz, 1C), 128.63 (s, 1C), 141.51 (s, 1C), 142.86 (s, 1C), 160.11 (s, 1C), 160.76 (s, 1C), 174.95 (s, 1C); LC-MS (Method A): Rt 1.82, [M+H] 461.
3-{2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid (157)
Compound 157 was prepared following route C. 1H NMR (400 MHz, DMSO-d6), □ ppm: 1.19 (d, J=7.4 Hz, 3H), 3.00-3.12 (m, 3H), 3.18 (dd, J=13.2, 5.3 Hz, 1H), 3.52 (dd, J=13.2, 7.1 Hz, 1H), 3.56-3.66 (bs, 2H), 4.24 (br. s., 2H), 5.11 (s, 2H), 7.08 (d, J=9.0 Hz, 2H), 7.35-7.43 (m, 4H), 7.81 (d, J=9.0 Hz, 2H), 10.57-11.92 (bs, 1H); 13C NMR (101 MHz, DMSO-d6), □ PPM: 16.44 (q, 1C), 19.19 (t, 1C), 35.09 (d, 1C), 49.1 (br. t., 1C), 49.6 (br. t., 1C), 57.17 (t, 1C), 68.72 (t, 1C), 115.59 (d, 2C), 119.50 (s, 1C), 127.60 (d, 2C), 128.45 (d, 2C), 128.52 (s, 1C), 129.51 (d, 2C), 132.58 (s, 1C), 135.69 (s, 1C), 142.79 (s, 1C), 160.22 (s, 1C), 160.80 (s, 1C), 174.92 (s, 1C); LC-MS (Method A): Rt 1.72, [M+H] 427.
3-{2-[4-(2,3-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid (175)
Compound 175 was prepared following route C. 1H NMR (400 MHz, DMSO-d6), δ ppm: 1.27 (d, J=6.6 Hz, 3H), 2.5-2.6 (m, 1H), 2.8-3.1 (m, 3H), 3.1-3.5 (bs, 2H), 3.6-3.7 (bs, 1H), 3.9-4.1 (bs, 2H), 5.28 (s, 2H), 7.0-7.2 (m, 2H), 7.2-7.3 (m, 1H), 7.4-7.5 (m, 2H), 7.9-8.0 (m, 2H), 12.16 (br. s., 1H); LC-MS (Method A): Rt 1.3, [M+H] 429.
3-{2-[4-(4-Trifluoromethyl-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid (271)
Compound 271 was prepared following route C. 1H NMR (400 MHz, DMSO-d6), □ ppm: 1.29 (d, J=7.2 Hz, 3H), 3.06-3.36 (m, 4H), 3.51-3.68 (m, 2H), 3.70-3.90 (bs, 1H), 4.18-458 (bs, 2H), 5.33 (s, 2H), 7.03 (dd, J=8.7, 2.2 Hz, 1H), 7.10 (dd, J=12.9, 2.2 Hz, 1H), 7.65-7.71 (m, 2H), 7.73-7.77 (m, 2H), 7.92 (t, J=8.7 Hz, 1H), 11.01 (br. s., 1H); LC-MS (Method A): Rt 1.56, [M+H] 479.
2-(4-Bromo-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-carboxylic acid t-butyl ester (28, Rb=H)
To a suspension of compound 20 (Rb=H, 14.3 g, 48.2 mmol) in DCM (300 ml) were added DIPEA (1 eq) and di-t-butyl dicarbonate (11.59 g, 53 mmol, 1.1 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The mixture was concentrated in vacuo to give crude compound pure 28 (Rb=H) which was used in the next step without further purification.
2-(4-Hydroxy-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-carboxylic acid t-butyl ester (29, Rb=H)
Compound 28 (Rb=H, 9 g, 20.4 mmol) was dissolved in 1,4-dioxan (90 ml) and to the solution was added potassium hydroxide (4.58 g, 81 mmol, in 90 ml water) and the solution was degassed. To the solution was added 2-di-t-butylphosphino-2′,4′,6′-triisopropylbiphenyl (346 mg, 0.82 mmol, 0.04 eq) and tris-(dibenzylideneaceton)-dipalladium(0) (373.5 mg, 0.41 mmol, 0.02 eq). The mixture was stirred at 80° C. for 16 hrs after which time LC-MS analysis showed that the conversion was complete. The mixture was cooled to room temperature, diluted with EtOAc, acidified to pH 6 with 0.1N HCl and extracted with EtOAc. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (DCM/MeOH, 97/3, v/v) to give compound pure 29 (Rb=H, 6 g, 83%).
2-[4-(4-Trifluoromethyl-benzyloxy)-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-carboxylic acid t-butyl ester (30, Ra=4CF3, Rb=H)
Compound 29 (Rb=H, 6 g, 17 mmol) was dissolved in DCM/water, 2/1, v/v (78 ml/mmol) and to this solution was added sodium hydroxide (27 ml, 2N, 3 eq). To this mixture was added tetrabutylammonium bromide (549 mg, 0.1 eq) and 4-trifluoromethyl-benzyl bromide (4.48 g, 18.75 mmol, 1.1 eq). The mixture was stirred for 16 hrs at room temperature after which time LC-MS analysis showed complete reaction. The mixture was diluted with DCM (200 ml), the layers were separated and the water layer was extracted with DCM. The organic layers were dried on MgSO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (eluent: EtOAc/petroleum ether, 1/3) to give compound pure 30 (Rb=H, 9.0 g, 96%) as a colorless foam.
2-[4-(4-Trifluoromethyl-benzyloxy)-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine (30, Ra=4CF3, Rb=H)
Compound 30 (9.6 g, 18.5 mmol) was dissolved in DCM (150 ml) and trifluoroacetic acid (8.5 ml, 111 mmol, 6 eq) was added. The solution was refluxed for 16 hrs after which time TLC analysis showed complete reaction. The mixture was neutralized with 5% aq. NaHCO3. The mixture was extracted with DCM (3×) and the combined organic layers were washed with brine, dried on Na2SO4 and concentrated in vacuo to give compound 27 (Ra=4CF3, Rb=H) which was used in the next step without further purification.
3-{2-[4-(4-Trifluoromethoxybenzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-cyclobutane carboxylic acid (306)
Compound 27 (Ra=4CF3, Rb=H 0.71 g, 1.8 mmol) was suspended in 1,2-dichloroethane (40 ml).
To this suspension was added 3-oxocyclobutanecarboxylic acid (0.27 g, 2.34 mmol, 1.3 eq) and sodium triacetoxy borohydride (0.61 g, 2.88 mmol, 1.6 eq). The mixture was stirred at room temperature for 16 hrs after which time TLC analysis revealed complete reaction. The solution was diluted with 5% NaHCO3 (15 ml/mmol) and the mixture was extracted with DCM. The combined organic layers were dried on Na2SO4, concentrated in vacuo and the residue was purified by silica gel column chromatography to furnish a mixture of cis and trans stereoisomers in a ratio of 2 to 1. A second silica gel column chromatography purification (DCM/MeOH, 9/1, v/v) resulted in the isolation of two enriched stereoisomer fractions. Compound 307-cis (Rf, 0.2, 0.46 g, 51%, cis/trans=95/5) and 306-trans (Rf 0.25, 0.21 g, 25%, cis/trans=5/95). 307-cis: 1H NMR (400 MHz, DMSO-d6): □ ppm 1.92-2.05 (m, 2H); 2.26-2.37 (m, 2H); 2.68 (t, J=4.5 Hz, 2H); 2.70-2.79 (m, 3H); 2.89-3.00 (m, 1H); 3.30-3.41 (bs, 2H); 5.21 (s, 2H) 7.14 (d, J=8.8 Hz, 2H); 7.41 (d, J=8.5 Hz, 2H); 7.61 (d, J=8.5 Hz, 2H); 7.87 (d, J=8.8 Hz, 2H); 12.15 (br. s., 1H); 306-trans: 1H NMR (400 MHz, DMSO-d6) □ ppm: 2.10-2.21 (m, 2H); 2.22-2.34 (m, 2H); 2.67 (t, J=4.8 Hz, 2H); 2.71-278 (m., 2H); 2.84-2.95 (m, J=9.6 Hz, 1H); 3.17 (quin, J=7.4 Hz, 1H); 3.31-3.40 (bs, 2H); 5.21 (s, 2H); 7.14 (d, J=8.8 Hz, 2H); 7.41 (d, J=8.5 Hz, 2H); 7.61 (d, J=8.5 Hz, 2H); 7.87 (d, J=8.8 Hz, 2H); 12.20 (br. s., 1H). LC-MS Rt 1.41, [M+H]=489.
3-{2-[4-(3,4-Difluorobenzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-cyclobutane carboxylic acid (277)
Compounds 278-cis and 277-trans were prepared as described for 306. 278-cis: 1H NMR (400 MHz, DMSO-d6): □ ppm 1.93-2.04 (m, 2H) 2.26-2.36 (m, 2H) 2.68 (t, J=4.8 Hz, 2H) 2.70-2.80 (m, 3H) 2.88-3.00 (m, 1H) 3.33-3.40 (bs, 2H) 5.16 (s, 2H) 7.14 (d, J=8.8 Hz, 2H) 7.29-7.38 (m, 1H) 7.47 (dt, J=10.7, 8.4 Hz, 1H) 7.56 (ddd, J=11.5, 8.0, 2.0 Hz, 1H) 7.87 (d, J=8.8 Hz, 2H) 12.15 (br. s., 1H); LC-MS (method A): Rt 1.33, [M+H]=441; 277-trans: 1H NMR (400 MHz, DMSO-d6): □ ppm 2.09-2.20 (m, 2H) 2.23-2.31 (m, 2H) 2.67 (t, J=4.5 Hz, 2H) 2.70-2.77 (m, 2H) 2.84-2.93 (m, 1H) 3.12-3.22 (m, 1H) 3.32-3.39 (bs, 2H) 5.16 (s, 2H) 7.14 (d, J=8.8 Hz, 2H) 7.30-7.37 (m, 1H) 7.47 (dt, J=10.8, 8.4 Hz, 1H) 7.56 (ddd, J=11.4, 8.0, 1.9 Hz, 1H) 7.87 (d, J=8.8 Hz, 2H) 12.20 (br. s., 1H); LC-MS (method A): Rt 1.31, [M+H]=441.
3-Bromo-N-benzyloxycarbonyl-4-piperidone (502)
To a cooled (0° C.) solution of N-benzyloxycarbonyl-4-piperidone (501, 27.3 g 117 mmol) in DCM (3 ml/mmol) was added DIPEA (25.5 ml, 146.2 mmol, 1.25 eg) and trimethylsilyl trifluoromethane sulfonate (25.4 ml, 141 mmol, 1.2 eg). The mixture was stirred for 30 minutes at 0° C. Next, N-bromosuccinimide (21.2 g, 119.3 mml, 1.02 eg) was added and stirring was continued for 16 hrs at room temperature. The reaction mixture was washed with 5% NaHCO3 and the organic layer was dried (MgSO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography (diethyl ether/petroleum ether, 1/1 to 1/0, v/v, Rf 0.1) giving 502 in a yield of 90% as a yellow oil.
2-(4-Methoxy-phenyl)-6,7-dihydro-4H-thiazolo[5,4-c]pyridine-5-carboxylic acid benzyl ester (504)
Compound 502 (2.0 g, 6.5 mmol) was dissolved in ethanol (20 ml). To the solution was added 4-methoxythiobenzamide (503, 1.09 g, 6.5 mmol, 1 eq) and the yellow mixture was refluxed for 17 hrs after which time TLC analysis showed full conversion of 502. The reaction mixture was concentrated in vacuo and the residue was dissolved in EtOAc, washed with 5% Na2SO4 and the organic layer was dried (MgSO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography (diethyl ether, Rf 0.3) giving compound 504 in a yield of 49% as a colorless oil. Compounds having a Rb substitute can be prepared by choosing a properly substituted (Rb)-4-methoxythiobenzamide (503).
4-(4,5,6,7-Tetrahydro-thiazolo[5,4-c]pyridine-2-yl)-phenol 505
Compound 504 (18.0 g, 47.3 mmol) was dissolved in ethanol (300 ml). To the cooled (−78° C.) solution was added boron tribromide (5 eg, 236 mmol) dropwise. After one hour cooling was removed and the mixture was stirred 16 hrs at room temperature. The reaction was quenched with MeOH, the mixture was concentrated in vacuo to give compound 505 as an oil which was used in the next step without further purification.
General Procedure for the Synthesis of Compounds 508.
Compounds 508 are prepared starting from compounds 505 in a similar fashion as described for the synthesis of compounds 7 and 21 (see schemes 1-4). As a typical example we describe the synthesis of compound 508 (Ra=F, Rb=H, Rc=C2).
3-[2-(4-(Hydroxy-phenyl)-6,7-dihydro-4H-thiazolo[5,4-c]pyridine-5-yl]-propionic acid t-butyl ester (506, Rb=H, Rc=C2)
To a suspension of 505 (Rb=H, 5.8 g, 25.1 mmol) in MeOH (100 ml) and DIPEA (5.1 ml, 30.1 mmol, 1.2 eq) was added t-butyl acrylate (4.4 ml, 30.1 mmol, 1.2 eq) and the mixture was refluxed for 16 hrs after which time TLC analysis (diethyl ether, Rf 0.2) showed full conversion of 505. The reaction mixture was concentrated in vacuo and the residue was dissolved in EtOAc, washed with 5% NaHCO3 and the organic layer was dried (Na2SO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography (diethyl ether) giving compound 506 (Rb=H, Rc=C2) in a yield of 79% as a white solid material.
3-[2-(4-(2-Fluoro-benzyloxy)-phenyl)-6,7-dihydro-4H-thiazolo[5,4-c]pyridine-5-yl]-propionic acid t-butyl ester (507, Ra=2F, Rb=H, Rc=C2)
To a suspension of 506 (Rb=H, Rc=C2, 0.5 g, 1.4 mmol) in DMA (4 ml) was added triphenyl phosphine (0.45 g, 1.7 mmol, 1.25 eg), diisopropyl azodicarboxylate (0.33 ml, 1.7 mmol, 1.25 eq) and 2-fluoro benzyl alcohol (0.17 ml, 1.56 mmol, 1.15 eg) and the mixture was stirred at room temperature for 16 hrs. The reaction mixture was diethyl ether (150 ml) and washed with 3×50 ml water. The combined organic layers were dried (Na2SO4) and subsequently concentrated under reduced pressure. The resulting oil was purified by silica gel chromatography (diethyl ether/petroleum ether, 2/1, v/v) giving compound 507 (Ra=2F, Rb=H, Rc=C2) in a yield of 67% as a white solid material.
3-[2-(4-(2-Fluoro-benzyloxy)-phenyl)-6,7-dihydro-4H-thiazolo[5,4-c]pyridine-5-yl]-propionic acid (38, Ra=2F, Rb=H, Rc=C2)
Compound 507, Ra=2F, Rb=H, Rc=C2, 0.43 g, 0.9 mmol) was dissolved in 4N HCl in dioxane (10 ml). The mixture was stirred for 16 hrs at room temperature. The mixture was concentrated in vacuo and the resulting solid was washed with diisopropyl ether to give 38 as a white solid (Ra=2F, Rb=H, Rc=C2, 0.42 g, 97%). Compound 38: 1H NMR (400 MHz, DMSO-d6), □ ppm 2.88-3.03 (m, 2H), 3.06-3.14 (m, 1H), 3.20 (m, 1H), 3.48 (broad signal, 3H), 3.73-3.87 (m, 1H), 4.44 (bd, J=15.9, 6.0 Hz, 1H), 4.72 (b, J=15.9 Hz, 1H), 5.22 (s, 2H), 7.16 (d, J=8.6 Hz, 2H), 7.23-7.32 (m, 2H), 7.41-7.49 (m, 1H), 7.59 (dt, J=7.6, 1.5 Hz, 1H), 7.87 (d, J=8.6 Hz, 2H), 11.63 (br. s., 1H); LC-MS (Method A): Rt 1.3, [M+H] 473.
4-Bromo-N-(4-chloro-pyridin-3-yl)-benzamide (510)
A mixture of 4-amino-3-chloropyridine (509, 5.9 g, 46.2 mmol), 4-bromobenzoyl chloride (15, 11.15 g, 50.8 mmol) and potassium carbonate (22.4 g, 161.7 mmol) in acetonitril (150 ml) was refluxed for 24 hrs. The reaction mixture was concentrated in vacuo, redissolved in DCM and the solution was washed with water. The organic layer was dried (MgSO4), concentrated and the resulting oil was purified by silica gel column chromatography (DCM/MeOH, 99/1, v/v, Rf 0.2) to give pure 510 (10.4 g, 72%) as an oil. When a substituted compound 15 is used, the Rb in compound 510 is introduced.
2-(4-Bromo-phenyl)-thiazolo-[4,5c]-pyridine (511)
Compound 510 (10.4 g, 33.4 mmol) was suspended in toluene (400 ml) and Lawesson's reagent (9.4 g, 23.4 mmol, 0.7 eq) was added and the mixture was refluxed for 24 hrs after which time TLC analysis (DCM/MeOH, 97/3, v/v, Rf 0.7) revealed the reaction to be complete. The mixture was concentrated in vacuo and to the oil was added NaHCO3 (5% solution, 200 ml) and the suspension was extracted with DCM (3×200 ml). The combined organic layers was dried over MgSO4, concentrated and the oil was purified by silica gel column chromatography (DCM/MeOH, 99/1 to 97/3, v/v) to give pure 511 (8.7 g, 89%) as an oil.
2-(4-Benzyloxy-phenyl)-4,5,6,7-tetrahydro-thiazolo-[4,5c]-pyridine (518)
Compounds 518 were obtained starting from 511 in a similar fashion as described for the synthesis of compounds 27 in schemes 4 and 7. The appropriate tails Rc could be linked to 518 in a similar fashion as described for compounds 27.
4-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-thiazolo-[4,5c]-pyridin-5-yl}-butyric acid (446, Ra=3,5-F, Rb=H, Rc=C3)
Compound 519 (Ra=3,5-F, Rb=H, Rc=C3, 0.79 g, 1.58 mmol) was dissolved in 4N HCl in dioxan (25 ml) was stirred for 18 hrs at 50° C. and for 70 hrs at room temperature. The mixture was concentrated in vacuo and the resulting oil was stirred in diethyl ether to yield 446 as a white solid (0.72 g, 94%). 1H NMR (400 MHz, DMSO-d6): □ ppm: 2.03-2.15 (m, 2H), 2.40 (t, J=7.3 Hz, 2H), 3.12-3.22 (m, 1H), 3.32 (broad signal, 3H), 3.40-3.51 (m, 1H), 3.75-3.90 (m, 1H), 4.36 (dd, J=15.2, 6.8 Hz, 1H), 4.59 (d, J=15.2 Hz, 1H), 5.21 (s, 2H), 7.05 (tt, J=9.0, 2.3 Hz, 1H), 7.09-7.18 (m, 4H), 7.84 (d, J=8.9 Hz, 2H), 11.17 (br. s., 1H); Rt 1.33, [M+H] 502.
2-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-acetic acid (463)
2-[4-(3,5-difluoro-benzyloxy)-phenyl]-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine (27, Ra=3,5-diF, Rb=H) was synthesized following route C as described above. Compound 27 (Ra=3,5-diF, Rb=H, 0.4 g, 1.17 mmol) was dissolved in acetonitril (20 ml). To the solution was added DIPEA (0.51 ml, 2.9 mmol, 2.5 eq) and t-butyl bromoacetate (0.19 ml, 1.29 mmol, 1.1 eq). The mixture was refluxed for 6 hrs after which time LCMS analysis showed the reaction to be complete. The mixture was concentrated in vacuo, water was added and the suspension was extracted with DCM (3×200 ml). The combined organic layers was dried over MgSO4, concentrated and the oil was purified by silica gel column chromatography (diethyl ether/petroleum ether, 3/1, v/v). The resulting oil was suspended in 4N HCl in dioxan (20 ml). The mixture was stirred at 45° C. for 5 hrs and at room temperature for 16 hrs. The mixture was concentrated in vacuo to give a white solid. The solid material was washed 3 times with diethyl ether to give 463 (0.39 g, 91%) as a white solid. 1H NMR (400 MHz, DMSO-d6): □ ppm: 3.10-3.20 (m., 2H), 3.70-3.80 (m., 2H), 4.31 (s, 2H), 4.44 (s, 2H), 5.22 (s, 2H), 7.02-7.10 (m, 1H), 7.13-7.20 (m, 4H), 7.92 (d, J=8.8 Hz, 2H), 9.53-12.44 (br.s., 1H); Rt 1.51; [M+H] 401.
2-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-propionic acid (464)
Compound 27 (Ra=3,5-diF, Rb=H, 0.4 g, 1.17 mmol) was dissolved in acetonitril (20 ml). To the solution was added DIPEA (0.51 ml, 2.9 mmol, 2.5 eq) and t-butyl 2-bromo-propionic ester (0.27 gl, 1.29 mmol, 1.1 eq). The mixture was refluxed for 24 hrs after which time LCMS analysis showed the reaction to be complete. The mixture was concentrated in vacuo, water was added and the suspension was extracted with DCM (3×200 ml). The combined organic layers were dried on MgSO4, concentrated and the oil was purified by silica gel column chromatography (diethyl ether/petroleum ether, 3/2, v/v, Rf 0.3). The resulting oil was suspended in 4N HCl in dioxan (20 ml). The mixture was stirred at 45° C. for 5 hrs and at room temperature for 16 hrs. The mixture was concentrated in vacuo to give a white solid. The solid material was washed 3 times with diethyl ether to give 464 (0.41 g, 89%) as a white solid. 1H NMR (400 MHz, DMSO-d6): □ ppm, 1.66 (d, J=7.1 Hz, 3H), 3.17 (br. s., 2H), 3.74 (br. s., 2H), 4.33-4.52 (m, 4H), 5.22 (s, 2H), 7.02-7.11 (m, 1H), 7.13-7.25 (m, 4H), 7.92 (d, J=8.8 Hz, 2H); Rt 1.53, [M+H] 415.
3-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-oxazolo[4,5-c]-pyridin-5-yl}-cyclopentane carboxylic acid (465, 1/1 cis-trans mixture)
Compound 27 (Ra=3,5-diF, Rb=H, 0.1 g, 0.29 mmol) was dissolved in 1,2-dichloroethane (5 ml). To the solution was added 3-oxo-cyclopentane-carboxylic acid (0.05 g, 0.41 mmol, 1.4 eq), sodium triacetoxy borohydride (0.11 g, 0.53 mmol, 1.8 eq) and AcOH (0.03 ml, 0.58 mmol, 2 eq). The mixture was stirred for 16 hrs after which time LCMS analysis showed the reaction to be complete. To the mixture was added a saturated solution of NH4Cl and the mixture was extracted with DCM. The combined organic layers was concentrated in vacuo and the oil was purified by silica gel column chromatography (DCM/MeOH, 9/1, v/v) to give compound 465 as a cis/trans mixture in a ratio of 1/1.
Rt 1.27, [M+H] 455.
4-{2-[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo-[4,5c]-pyridin-5-yl}-pentanoic acid (393)
Compound 22 (Rb=2Me, Rc=H) was prepared by stirring 29 (Rb=2Me) in a mixture of TFA and DCM for 16 hrs. The solvents were evaporated to give crude 22 (Rb=2Me, Rc=H) as an oil which was used in the next step without further purification. A SCX-2 column was used to make the free base of compound 22 (Rb=2Me, Rc=H, 1 g, 4.3 mmol), which was dissolved in MeOH. To this solution was added ethyl levulinate (2.46 ml, 17.4 mmol, 4 eq), AcOH (0.5 ml, 8.7 mmol, 2 eq) and palladium hydroxide on carbon. The reaction mixture was shaken at a pressure of 4 atm of hydrogen. After 16 hrs, ELSD showed that the reaction was not yet complete. Another portion of ethyl levulinate (2.46 ml, 17.4 mmol, 4 eq) and palladium hydroxide on carbon was added and hydrogenation at 4 atm was continued for 72 hrs. The mixture was degassed, filtered over Hyflo and the residue was washed with MeOH. The filtrate was concentrated in vacuo and the resulting oil was purified by silica gel column chromatography to give 522 (Rb=2Me, Rc=xCH2(CH3)CH2CH2C(═O)OEt) in an yield of 41%. Subsequent benzylation of 522 with 2,3-di-fluoro-benzyl bromide was performed in a similar fashion as described for the compounds of Schemes 1-5 giving compound 523. Finally, the latter was dissolved in a solution of sodium hydroxide in ethanol (18 ml, 0.3M) and the solution was stirred at 50° C. for 16 hrs. The mixture was concentrated in vacuo and the residue was stirred in diethyl ether to give 393 as a white solid (Ra=2,3-diF, Rb=2Me, Rc=CH2(CH3)CH2CH2C(═O)OH). 1H NMR (400 MHz, DMSO-d6): □ ppm 1.40 (d, J=5.9 Hz, 3H), 1.74-1.93 (m, 1H), 2.15-2.30 (m, 1H), 2.31-2.50 (m, 3H), 2.63 (s, 3H), 3.04-3.15 (m, 1H), 3.22-3.35 (m, 1H), 3.45-3.69 (m, 2H), 3.72-3.85 (m, 1H), 4.22-4.46 (m, 2H), 5.25 (s, 2H), 6.98-7.07 (m, 2H), 7.20-7.29 (m, 1H), 7.34-7.45 (m, 2H), 7.87 (d, J=8.6 Hz, 1H), 10.49-10.69 (br.band, 1H), 11.47-13.29 (br.band, 1H). Rt 1.37, [M+H] 457.
4-{2-[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo[4,5-c]-pyridin-5-yl}-2-methyl-butyric acid (394)
Compound 22 (Rb=2Me, 1 g, 3.8 mmol) was dissolved in acetonitril (10 ml) and to the solution was added DIPEA (1.93 ml, 11.25 mmol), sodium iodide (0.56 g, 3.75 mmol) and 4-chloro-2-methylbutyric acid methyl ester (0.85 g, 5.6 mmol). The mixture was stirred at 60° C. for 16 hrs, after which time again added DIPEA (1.93 ml, 11.25 mmol), sodium iodide (0.56 g, 3.75 mmol) and 4-chloro-2-methylbutyric acid methyl ester (0.85 g, 5.6 mmol) were added and stirring was continued for 30 hrs at 60° C. The mixture was concentrated in vacuo and purified by silica gel column chromatography (DCM/MeOH, 95/5 to 9/1, v/v) to give 525 (Rb=2Me; Rd=Me) as an oil. Compound 525 (Rb=2Me; Rd=Me) was benzylated at Mitsunobu conditions in a similar fashion as described above for the synthesis of 23. The resulting compounds 526 (0.5 mmol) were dissolved in ethanol (20 ml) and 2N NaOH solution was added (4 ml) and the mixture was stirred for 2 hrs at 50° C. The solution was neutralized with 1M HCl, extracted with DCM (3×20 ml) and the organic layers were dried on MgSO4. Evaporation of the solvent resulted in the isolation of pure compound 394. 1H NMR (400 MHz, DMSO-d6): □ ppm 1.09 (d, J=7.1 Hz, 3H), 1.51-1.61 (m, 1H), 1.80-1.92 (m, 1H), 2.36-2.46 (m, 1H), 2.56 (t, J=7.1 Hz, 2H), 2.60 (s, 3H), 2.72-2.78 (m, 2H), 2.79-2.85 (m, 2H), 3.39-3.47 (m, 2H), 5.24 (s, 2H), 6.95-7.04 (m, 2H), 7.20-7.29 (m, 1H), 7.35-7.47 (m, 2H), 7.82 (d, J=8.6 Hz, 1H), 10.85-13.06 (br.band, 1H). LC-MS: Rt 1.38, [M+H] 457.
4-{2-[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo-[4,5c]-pyridin-5-yl}-3-methyl-butyric acid (437)
Synthesis of 4-Chloro-3-methyl-butyric acid methyl ester
4-Methyl-dihydro-furan-2-one (4 g, 39.95 mmol) was dissolved in MeOH (10 ml) and the solution was cooled to −10° C. Thionyl chloride (3.6 ml, 49.9 mmol) was added dropwise. The mixture was stirred at −10° C. for 2 hrs and hereafter for 16 hrs at room temperature. The mixture was concentrated in vacuo to give crude 4-Chloro-3-methyl-butyric acid methyl ester (4 g, 87%) which was used in the next step without further purification.
Compound 22 (Rb=2Me, Rd=Me) was selectively alkylated at the nitrogen position with 4-chloro-3-methyl-butyric acid methyl ester to give 528. Subsequent benzylation of 528 under previously described Mitsunobu conditions gave 529. Compound 529 was demethylated in a similar fashion as described above for the synthesis of compound 394 to give compound 437 (Ra=2,3-diF, Rb=2Me, Rd=H) as a white solid. 1H NMR (400 MHz, DMSO-d6): □ ppm 1.12 (d, J=6.6 Hz, 3H), 2.26 (dd, J=16.3, 7.3 Hz, 1H), 2.44-2.57 (m, 2H), 2.63 (s, 3H), 2.99-3.96 (m, 6H), 4.10-4.65 (br. b., 2H), 5.24 (s, 2H), 6.96-7.08 (m, 2H), 7.18-7.27 (m, 1H), 7.31-7.43 (m, 2H), 7.88 (d, J=8.6 Hz, 1H), 10.17-11.21 (br.band, 1H), 11.46-13.30 (br.band, 1H). LC-MS: Rt 1.44, [M+H] 457.
3-{2-[4-(2-phenyl-cyclopropyl)-3-fluoro-phenyl]-6,7-dihydro-4H-oxazolo-[4,5-c]-pyridin-5-yl}-cyclobutane carboxylic acid (459 trans)
2-Phenyl-cyclopropyl-trifluoroborane. 4,4,5,5-Tetramethyl-2-(2-phenyl-cyclopropyl)-[1,3,2]dioxaborolane (3.1 g, 12.7 mmol) in MeOH (48 ml) and water (12 ml) was cooled to 0° C. Potassium bifluoride (6.96 g, 88.9 mmol, 7 eq) was added and the mixture was stirred for 16 hrs at room temperature. The mixture was concentrated in vacuo and the residue was co-evaporated with acetonitril (3×40 ml). The residue was washed with warm acetonitril (3×40 ml) and the combined actonitril washings were concentrated to give 2-phenyl-cyclopropyl-trifluoroborane (2 g, 71%). This trifluoroborane derivative (1.8 g, 8.2 mmol, 1.3 mmol) was dissolved in degassed toluene/water (137.5 ml, 10/1, v/v) and potassium phosphate tribasic (5.2 g, 24.7 mmol, 3.9 eq) was added. The mixture was stirred for 15 minutes and to this solution was added compound 28 (Rb=3F, 2.5 g, 6.3 mmol), palladium(II) acetate (71.2 mg, 0.3 mmol, 0.05 eq) and 2-dicyclohexylphosphino-2,6-di-isopropoxy-1,1-biphenyl (296 mg, 0.6 mmol, 0.1 eq, RuPhos). The mixture was stirred at 115° C. for 3 hrs after which time TLC analysis (Et2O/PA, 3/7, v/v) revealed the reaction to be complete. The mixture was allowed to reach room temperature and was diluted with EtOAc (300 ml) and the solution was washed with water. The organic layer was dried on MgSO4 and concentrated in vacuo to give an oil which was purified by silica gel column chromatography (Et2O/PA, 3/7, v/v) to give pure 530 (2.54 g, 92%) as a white solid. The BOC in 530 was removed under acidic conditions to give 531, which was transformed to 532 under conditions for introducing the Rc-tail as described above. Compound 532 (pure trans, 0.45 g, 0.94 mmol) was dissolved in 20 ml 4M HCl in dioxan. The solution was stirred for 16 hrs at room temperature. The mixture was concentrated in vacuo and stirred in diethyl ether. The resulting solid was filtered to give compound 459 (trans, 0.36 g, 83%). 1H NMR (400 MHz, DMSO-d6): □ ppm 1.27 (d, J=7.3 Hz, 3H), 1.51-1.70 (m, 2H), 2.26-2.40 (m, 2H), 3.05-3.23 (m, 3H), 3.27 (dd, J=13.1, 4.8 Hz, 2H), 3.55-3.84 (m, 3H), 4.37 (br. s., 2H), 7.14-7.23 (m, 3H), 7.26-7.35 (m, 3H), 7.64 (d, J=11.1, Hz, 1H), 7.74 (d, J=8.3 Hz, 1H), 10.00-11.79 (br.band, 1H), 11.93-13.53 (br.band, 1H). LC-MS: Rt 1.36, [M+H] 433.
4-(2-{4-[2-(3,5-Difluoro-phenyl)-vinyl]-phenyl}-6,7-dihydro-4H-oxazolo-[4,5c]-pyridin-5-yl)-butyric acid (451)
A solution of compound 28 (Rb=H, 4.3 g, 11.1 mmol), trans-2-(3,5-difluorophenyl)vinyl boronic acid pinacol ester (4.09 ml, 16.7 mmol, 1.5 eq) in toluene (100 ml) was degassed. To this solution was added chloro(2-dicyclohexylphosphino-2,4,6-tri-isopropopyl-1,1-biphenyl)[2-(2-aminoethyl)pentyl]palladium(II) methyl-tbutyl ether adduct (183.8 mg, 0.22 mmol, 0.02 eq) and potassium phosphate tribasic (7.08 g, 33.3 mmol, 3.0 eq) was added. The mixture was stirred for 24 hrs at 115° C. after which time LC-MS analysis revealed the reaction to be complete. The mixture was allowed to reach room temperature and was diluted with EtOAc (300 ml) and the solution was washed with a 5% NaHCO3 solution. The organic layer was dried on MgSO4 and concentrated in vacuo to give an oil which was purified by silica gel column chromatography (DCM/MeOH, 99.5/0.5, v/v) to give pure 533 (3.4 g, 69%) as a white solid. The BOC in 533 was removed under acidic conditions to give 534, which was transformed to 535 under conditions for introducing the Rc-tail as described above. Compound 535 (0.45 g, 0.94 mmol) was dissolved in 20 ml 4M HCl in dioxan. The solution was stirred for 16 hrs at 55° C. The mixture was concentrated in vacuo and stirred in diethyl ether. The resulting solid was filtered to give compound 451 (0.426 g, 95%).
1H NMR (400 MHz, DMSO-d6) □ ppm 2.02-2.14 (m, 2H) 2.40 (t, J=7.1 Hz, 2H) 3.07-3.18 (m, 1H) 3.23-3.38 (m, 3H) 3.65-3.72 (m, 1H) 3.78-3.89 (m, 1H) 4.23-4.35 (m, 1H) 4.41-4.54 (m, 1H) 6.96-7.05 (m, 1H) 7.28-7.37 (m, 3H) 7.41 (d, J=16.4 Hz, 1H) 7.74 (d, J=8.3 Hz, 2H) 7.98 (d, J=8.3 Hz, 2H) 11.25 (br. s., 1H). LC-MS: Rt 1.39, [M+H] 425.
4-(2-{4-[2-(3,5-Difluoro-phenyl)-ethyl]-phenyl}-6,7-dihydro-4H-oxazolo[4,5c]pyridin-5-yl)-butyric acid (452)
Compound 533 (1.5 g, 3.4 mmol) was dissolved in MeOH (250 ml). To the suspension was added palladium hydroxide on carbon (20%, 0.2 g, 1.42 mmol, 0.42 eq). The mixture was placed under a blanket of hydrogen at room temperature and 1 atmosphere. After 24 hrs, LC-MS analysis revealed the reaction to be complete (TLC analysis: DCM/MeOH, 99/1, v/v). The mixture was filtered over Hyflo and the filtrate was concentrated in vacuo to give 536 (1.43 g, 95%) as an oil. Compound 536 was converted to the corresponding tail derivative 538 as described above. The tBu or methyl esters were deprotected in a similar fashion as described above. Compound 452: 1H NMR (400 MHz, DMSO-d6) □ ppm 1.99-2.13 (m, 2H) 2.39 (t, J=7.2 Hz, 2H) 2.96 (s, 4H) 3.18 (broad band, 2H) 3.25-3.33 (m, 2H) 3.51-3.95 (broad band, 2H) 4.34 (br. s., 2H) 6.85-6.98 (m, 3H) 7.37 (d, J=8.3 Hz, 2H) 7.88 (d, J=8.3 Hz, 2H) 10.67-11.61 (broad band, 1H) 11.86-12.71 (broad band, 1H). LC-MS: Rt 1.36, [M+H] 427.
Preparation of the Pure Enantiomers of Chiral Compounds 1 and 2.
Compounds having for example a methyl substitution in the Rc-tail give mixtures of enantiomers as the test compound. Separation of this mixture in the pure enantiomers can be performed by chiral HPLC techniques from the moment that the chiral tail is introduced in the core, i.e. of for example compounds 1, 2, 7, 8, 9, 21, 22, 23. For those skilled in the art, it is obvious that small changes in the core can lead to a different behavior in the chiral separation process. Therefore, each compound has been screened using a broad set of conditions such as chiral column material and eluents. In that way, for each compound was determined in which stage and under which chiral separation conditions the separation would be most successful. The separated end products were named as Rel1 and Rel2 as the absolute configuration was not yet determined. This process is illustrated by the following typical examples.
3-[2-(4-Hydroxy-phenyl)-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine]-2-methyl-propionic acid t-butyl ester (+) and (−) enantiomers of 22b (Rb=H)
Compound 22b was made as an enantiomeric mixture as described in Scheme 5. Enantiomeric mixture was separated by chiral HPLC using the following chiral HPLC system. Stationary phase: Chiralcel OD-H (5 micron); mobile phase: n-heptane/2-propanol (90/10, v/v) +0.1% TFA; flowrate 1 ml/min; detection by UV at 280 nm. Compound 22b-rel1: [α]25=+37.1; 1H NMR (400 MHz, Chloroform-d) □ ppm 1.14 (d, J=6.8 Hz, 3H) 1.43 (s, 9H) 2.54 (dd, J=12.1, 6.2 Hz, 1H) 2.60-2.70 (m, 1H) 2.73-2.77 (m, 2H) 2.80-2.97 (m, 3H) 3.49 (d, J=14.1 Hz, 1H) 3.60 (d, J=14.1 Hz, 1H) 6.71-6.96 (br.band, 1H) 6.84 (d, J=8.6 Hz, 2H) 7.82 (d, J=8.6 Hz, 2H). Compound 22-rel2: [α]25=−35.9; 1H NMR (400 MHz, Chloroform-d) □ ppm 1.14 (d, J=6.8 Hz, 3H) 1.42 (s, 9H) 2.53 (dd, J=12.4, 6.2 Hz, 1H) 2.60-2.70 (m, 1H) 2.72-2.79 (m, 2H) 2.80-2.96 (m, 3H) 3.49 (d, J=14.1 Hz, 1H) 3.59 (d, J=14.1 Hz, 1H) 6.83 (d, J=8.6 Hz, 2H) 7.45-7.75 (br.band, 1H) 7.79 (d, J=8.6 Hz, 2H).
3-[2-(4-hydroxy-3-F-phenyl)-4,5,6,7-tetrahydro-oxazolo[4,5-c]pyridine]-2-methyl-propionic acid t-butyl ester (+) and (−) enantiomers of 22b (Rb=3F)
Compound 22b (Rb=H) was made as an enantiomeric mixture as described in Scheme 5. Enantiomeric mixture was separated by chiral HPLC using the following chiral HPLC system. Stationary phase: Chiralcel OD-H (5 micron); mobile phase: n-heptane/2-propanol (90/10, v/v) +0.1% TFA; flowrate 1 ml/min; detection by UV at 280 nm. Compound 22b-rel1: [α]25=−36.4 (MeOH). Compound 22-rel2: [α]25=+34.8; 1H NMR (400 MHz, Chloroform-d) □ ppm 1.15 (d, J=6.8 Hz, 3H) 1.43 (s, 9H) 2.54 (dd, J=11.9, 6.2 Hz, 1H) 2.60-2.71 (m, 1H) 2.73-2.79 (m, 2H) 2.81-2.96 (m, 3H) 3.49 (d, J=14.1 Hz, 1H) 3.59 (d, J=14.1 Hz, 1H) 4.88-6.89 (br.band, 1H) 7.02 (t, J=8.5 Hz, 1H) 7.61-7.73 (m, 2H).
3-{2-[4-(2,3-difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid rel 1 and rel 2 of compound 175
The tbutylprotected derivative of 175 was separated in its pure enantiomers by chiral HPLC. Stationary phase: Chiralpak IC (5μ); column code no.: WJH022830; dimensions: 250×4.6 mm; Mobile phase: n-Heptane/DCM/ethanol (50/50/1)+0.1% DEA; flowrate: 1 ml/min; Injection: 5 μl; Detection: UV (290 nm). Deprotection of thus obtained pure enantiomers resulted in the isolation of the test compounds 426 and 425. Compound 426, rel1: [α]25=−22 (MeOH), Rt 1.3, [M+H] 429. Compound 425 rel2: [α]25=+19.9 (MeOH); Rt 1.3, [M+H] 429.








TABLE 1








A









 



B









 



C









 



D









 



E









 

























RT (min)


No.
R1
Z
R2
X
Y
R3
Structure
R4
LC-MS





 31
4Cl-phenyl
CH2N
H
C
O
H
B
—(CH2)2—COOH
1.37


 32
cyclohexyl
CH2O
H
C
O
H
B
—(CH2)3—COOH
1.46


 33
2F-phenyl
CH2O
H
C
O
H
B
—(CH2)2—COOH
1.39


 34
2,6diCl-phenyl
CH2O
H
C
O
H
B
—(CH2)3—COOH
1.4


 35
2F-phenyl
CH2O
H
C
O
H
B
—(CH2)3—COOH
1.37


 36
2F-3pyridyl
CH2O
H
C
O
H
B
—(CH2)2—COOH
1.21


 37
phenyl
CH2
H
C
O
H
B
—(CH2)2—COOH
1.38


 38
2F-phenyl
CH2O
H
N
S

D
—(CH2)2—COOH
1.3


 39
phenyl
N
H
C
O
H
B
—(CH2)2—COOH
1.31


 40
2,6diCl-phenyl
CH2O
H
N
S

D
—(CH2)3—COOH
1.35


 41
phenyl
C═C
H
C
O
H
B
—(CH2)2—COOH
1.42


 42
phenyl
(CH2)2
H
C
O
H
B
—(CH2)2—COOH
1.45


 43
phenyl
SO2
H
C
O
H
B
—(CH2)2—COOH
#


 44
phenyl
S
H
C
O
H
B
—(CH2)2—COOH
1.43


 45
benzyl
S
H
C
O
H
B
—(CH2)2—COOH
1.41


 46
phenyl
O
H
C
O
H
B
—(CH2)2—COOH
1.37


 47
phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.32


 48
2F-phenyl
CH2O
2Cl
C
O
H
B
—(CH2)2—COOH
1.43


 49
phenyl
CH2SO2
H
C
O
H
B
—(CH2)2—COOH
1.16


 50
phenyl
CH2O
3-pyr
C
O
H
B
—(CH2)2—COOH
1.27


 51
2F-phenyl
CH2O
3F
C
O
H
B
—(CH2)2—COOH
1.34


 52
phenyl
C═O
H
C
O
H
B
—(CH2)2—COOH
1.23


 53
phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.17


 54
2F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.18


 55
2,3-diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.19


 56
2F-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.15


 57
2,3-diF-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.16


 58
4Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.37


 59
2F-phenyl
CH(Me)—O
H
C
O
H
B
—(CH2)3—COOH
#


 60
2,6-diCl-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.32


 61
4Cl-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.29


 62
2,6-diCl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.36


 63
2F-phenyl
NH
H
C
O
H
E
—(CH2)2—COOH
1.33


 64
2F-phenyl
CH2O
H
C
O
H
A
—(CH2)2—COOH
1.32


 65
2F-phenyl
bond
H
C
O
H
E
—(CH2)2—COOH
1.33


 66
2F-phenyl
O
H
C
O
H
E
—(CH2)2—COOH
1.34


 67
2F-phenyl
CH2O
H
C
O
H
E
—(CH2)2—COOH
1.36


 68
2F-phenyl
CH2N
H
C
O
H
E
—(CH2)2—COOH
1.29


 69
2F-phenyl
CH2O
H
C
O
CH3
B
—(CH2)2—COOH
1.38


 70
2F-phenyl
CH2O
H
C
O
H
C
—(CH2)2—COOH
1.35


 71
2F-phenyl
CH2O
H
C
O
H
A
—(CH2)2—COOH
1.32


 72
2F-phenyl
CH2O
H
C
O
H
D
—(CH2)2—COOH
1.36


 73
2F-phenyl
CH2O
2F
C
O
H
B
—(CH2)3—COOH
1.35


 74
4CF3-phenyl
CH2O
H
C
O
H
B
—(CH2)2—COOH
#


 76
phenyl
CH2O
H
C
O
H
B
—CH(CH3)—CH2—COOH
1.46


 77
4Cl-phenyl
CH2O
H
C
O
H
B
—CH2—CH(CH3)—COOH
1.56


 79
2F-phenyl
cyclopropyl
H
C
O
H
B
—(CH2)2—COOH
1.46


 80
2F-phenyl
OCH2
H
C
O
H
B
—(CH2)2—COOH
1.39


 81
phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.54


 82
phenyl
C≡C
H
C
O
H
E
—(CH2)2—COOH
1.63


 83
2F-phenyl
C≡C
H
C
O
H
E
—(CH2)2—COOH
1.65


 84
3F,5Cl-phenyl
CH2O
H
C
O
H
B
—(CH2)2—COOH
1.8


 85
4CF3-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.77


 86
4Cl-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.65


 87
2F-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.58


 88
2,3-diF-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.59


 89
2F-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.61


 90
phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.69


 91
4Cl-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.75


 92
2,3-diF-phenyl
O
2CH3
N
O

B
—(CH2)3—COOH
1.77


 93
3,4-diF-phenyl
O
2CH3
N
O

B
—(CH2)3—COOH
1.8


 94
4CF3-phenyl
O
2CH3
N
O

B
—(CH2)3—COOH
1.98


 95
2CH3-phenyl
O
H
N
O

B
—(CH2)2—COOH
2.09*


 96
4CH3-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.97*


 97
4CH3O-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.93*


 98
4CF3-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
2.1*


 99
4CF3O-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
2.08*


100
4F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.98*


101
3CH3O-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.86*


102
3F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.87*


103
3CF3O-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
2*


104
3CF3-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
2.04*


105
2,6diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.82*


106
2,5diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.83*


107
2F,6Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.84*


108
2,3,6triF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.82*


109
3,4diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.84*


110
2,4diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.84*


111
3CH3-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.86*


112
3,5diF-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.85*


113
2CF3-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.88*


114
3,4diF-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.68


115
2F-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.53


116
phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.53


117
4Cl-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.59


118
2,3diF-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.52


119
3,4diF-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.74


120
4CF3-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.8


121
4CH3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.86*


122
4CF3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.89*


123
4CF3O-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.93*


124
4F-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.83*


125
3CH3O-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.82*


126
3F-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.82*


127
4Cl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.88*


128
3CF3O-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.91*


129
2F-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.82*


130
3CF3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.9*


131
2CH3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.85*


132
2,6diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.8*


133
2,5diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.84*


134
2F,6Cl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.89*


135
2,3,6triF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.84*


136
2,6diCl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.87*


137
2,3diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.84*


138
3,4diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.86*


139
2,4diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.86*


140
2,5diCl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.91*


141
3CH3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.86*


142
3,5diF-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.84*


143
2Cl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.9*


144
2CF3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.89*


145
3,4diCl-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.95*


146
phenyl
CH2O
H
N
O

B
—CH2—CH(CH3)—COOH
1.48


147
phenyl
CH2O
H
N
O

D
—(CH2)2—COOH
1.28


148
3,4diF-phenyl
CH2O
3Me
C
O

B
—(CH2)3—COOH
1.68


149
4CF3-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.63


150
2CH3-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.48


151
3,4diF-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.56


152
4F-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.55


153
3,5diF-phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.48


154
2,3diF-phenyl
CH2O
H
N
O

B
—CH2—CH(CH3)—
1.32


155
3,4diF-phenyl
CH2O
H
N
O

B
—CH2—CH(CH3)—
1.63


156
4CF3-phenyl
CH2O
H
N
O

B
—CH2—CH(CH3)—COOH
1.82


157
4Cl-phenyl
CH2O
H
N
O

B
—CH2—CH(CH3)—COOH
1.72


159
phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.87*


160
4CF3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
2.03*


161
4CF3O-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
2.03*


162
4F-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


163
3CH3O-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.85*


164
3F-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


165
4Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.94*


166
3CF3O-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.97*


167
2F-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.92*


168
3CF3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
2*


169
3Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.95*


170
2CH3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.91*


171
4CH3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.92*


172
2,6-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


173
2F,6Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.92*


174
2,6diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.97*


175
2,3-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.3*


176
3,4-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


177
2,4-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


178
2,5-diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.99*


179
3CH3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.93*


180
3,5-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.9*


181
2CF3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.96*


182
4-CH3O-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
2.0*


183
3,4-diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.6


184
4pyridinyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.51*


185
2F,4Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.95*


186
2F,4CH3-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.95*


187
4CN-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.84*


188
2,3,6-triF-
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


189
2,5-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.89*


190
chexyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
2.05*


191
2Cl,4F-phenyl
CH2O
H
C
O
H
B
—(CH2)3—COOH
1.43


192
2,3-diCl-phenyl
CH2O
H
C
O
H
B
—(CH2)3—COOH
1.48


193
phenyl
CH2O
H
N
O

B
—(CH2)3—COOH
1.24


194
2F-phenyl
CH2O
H
N
O

B
—C[(CH2)2]—CH2—COOH
1.87


195
4CF3O-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.47


196
4F-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.35


197
phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.3


198
4Cl-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.37


199
2,4diF-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.36


200
4Me-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.39


201
3,4diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.35


202
3,5diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.35


203
4CF3-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.41


204
2F-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.28


205
2,3diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.32


206
4CF3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.05*


207
4F-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.97*


208
3CH3O-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.25*


209
3F-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.1*


210
3CF3O-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.06*


211
4Cl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.03*


212
2F-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.96*


213
3Cl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2*


214
4CH3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.02*


215
2,6diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.94*


216
2,3,6triF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.98*


217
2,6diCl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.03*


218
2,3diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.03*


219
2,5diCl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.06*


220
3,4diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2*


221
3,4diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.98*


222
3CH3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.03*


223
2CF3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.03*


224
4CH3O-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.96*


225
3,5diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.99*


226
2,4diF-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.98*


227
3,4diCl-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.42


228
2F,4CH3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.86*


229
3,4diCl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.07*


230
3,5diF-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.33


231
2CH3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.99*


232
2Cl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.01*


233
4F-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.38


234
2,4diF-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.38


235
3CF3-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.39


236
4CF3O-phenyl
CH2O
3CH3O
N
O

B
—(CH2)2—COOH
1.44


237
2F-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.26


238
3CF3-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
2.06*


239
2F,6Cl-phenyl
CH2O
3F
N
O

B
—(CH2)3—COOH
1.99*


240
2,3diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.26


241
3CF3O-phenyl
CH2O
2F
N
O

B
—(CH2)3—COOH
1.42


242
2F-phenyl
CH2O
H
N
O

B
—CH2—CF2—COOH
2.16


243
4CH3-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.37


244
4F-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.33


245
2,4diF-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.33


246
3,4diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.3


247
3,5diF-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.3


248
4Cl-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.32


249
4CF3-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.38


250
4CF3O-phenyl
CH2O
3CH3O
N
O

B
—(CH2)3—COOH
1.38


251
3CF3-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.4


252
3,4diCl-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.45


253
3CF3O-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.48


254
4Cl-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.38


255
3,4diF-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.36


256
3,5diF-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.39


257
4CF3-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.42


258
2F-phenyl
CH2O
3F
N
O

B
—(CH2)2—COOH
1.35


259
4CF3-phenyl
CH2O
3F
N
O

B
—(CH2)2—COOH
3.02


260
3,5diF-phenyl
CH2O
3F
N
O

B
—(CH2)2—COOH
2.98


261
2F-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
2.94


262
3,5diF-phenyl
CH2O
2CH3
N
O

B
—(CH2)3—COOH
1.66


263
2,3diF-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.33


264
4CF3O-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.46


265
3,5diCl-phenyl
CH2O
2Cl
N
O

B
—(CH2)3—COOH
1.49


266
3,5diF-Phenyl
CH2O
2Cl
N
O

B
—(CH2)2—COOH
1.42


267
4CF3-phenyl
CH2O
2Cl
N
O

B
—(CH2)2—COOH
1.5


268
2F-phenyl
CH2O
2Cl
N
O

B
—(CH2)2—COOH
1.36


269
2,6diCH3-phenyl
CH2O
2F
N
O

B
—(CH2)2—COOH
1.36


271
4CF3-phenyl
CH2O
2F
N
O

B
—CH2—CH(CH3)—COOH
1.56


272
2,6diCH3-phenyl
CH2O
2CH3
N
O

B
—(CH2)2—COOH
1.46


273
3,4diF-phenyl
CH2O
2CH3O
N
O

B
—CH(CH3)—CH2—COOH
1.42


274
4CF3-phenyl
CH2O
2CH3O
N
O

B
—CH(CH3)—CH2—COOH
1.51


275
2F-phenyl
CH2O
2CH3O
N
O

B
—CH(CH3)—CH2—COOH
1.37


276
3,5diF-phenyl
CH2O
2CH3O
N
O

B
—CH(CH3)—CH2—COOH
1.42


277
3,4diF-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.31


278
3,4diF-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.33


279
4Cl-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.36


280
4Cl-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.37


281
4F-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.03*


282
4CF3-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.11*


283
2F-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
1.99*


284
4CF3O-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.1*


285
4Cl-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.07*


286
3CF3O-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.14*


287
3,4diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.03*


288
2,4diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.02*


289
3,4diCl-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.16*


290
3,5diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.03*


291
2Cl-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.05*


292
2F,4Cl-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.06*


293
3CF3-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.09*


294
2,5diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.02*


295
4CF3-phenyl
CH2O
2F
N
O

B
—CH(CH3)—CH2—COOH
1.56


296
4CF3O-phenyl
CH2O
2CH3O
N
O

B
—CH(CH3)—CH2—COOH
1.54


298
3,4diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)2—COOH
1.44


299
2,3diF-phenyl
CH2O
3Cl
N
O

B
—(CH2)3—COOH
2.01*


301
2F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.38


302
2F-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.29


303
2F-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.3


304
4-fluoro-indan-2-yl
O
H
N
O

B
—(CH2)2—COOH
1.39


305
indan-2-yl
O
H
N
O

B
—(CH2)2—COOH
1.37


306
4CF3O-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.41


307
4CF3O-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.43


308
indan-1-yl
O
H
N
O

B
—(CH2)2—COOH
1.39


309
4-fluoro-indan-1-yl
O
H
N
O

B
—(CH2)2—COOH
1.4


310
4Cl-phenyl
CH2O
3Cl
N
O

B
—(CH2)2—COOH
1.48


311
3,4diF-phenyl
CH2O
2F
N
O

B
—CH(CH3)—CH2—COOH
1.45


312
4F-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.28


313
4F-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.3


314
4CF3-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.41


315
4CF3-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH
1.42


316
3F,4Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.52


318
2Cl,4F-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.5


319
2F,3Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.49


320
3F,5Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.54


322
2,3diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.59


323
2Cl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.48


324
2,4diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.6


325
3,5diCl-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH
1.63


326
3,5diF-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH (trans)
1.34


327
3,5diF-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH(cis)
1.34


328
3,4diCl-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH (trans)
1.45


329
3,4diCl-phenyl
CH2O
H
N
O

B
-cyclobutyl-COOH(cis)
1.46


330
4Cl-phenyl
CH2O
H
N
NH

B
—CH(CH3)—CH2—COOH
1.16


331
3,4diF-phenyl
CH2O
H
N
NH

B
—CH(CH3)—CH2—COOH
1.11


332
3,5diCl-phenyl
CH2O
H
N
NH

B
—CH(CH3)—CH2—COOH
1.11


349
4Cl-phenyl
CH2O
H
C
S
H
B
—(CH2)2—COOH
#)


350
4CF3-phenyl
CH2O
H
C
S
H
B
—(CH2)2—COOH
1.57


352
3,5-diCl-phenyl
CH2O
H
C
S
H
B
—(CH2)2—COOH
1.6


354
3,5-diF-phenyl
CH2O
H
C
S
H
B
—(CH2)2—COOH
1.46


357
3,4-diCl-phenyl
CH2O
3Cl
N
O

B
—(CH2)2—COOH
1.56


359
4-Cl-phenyl
CH2O
3Cl
N
O

B
—CH(CH3)—CH2—COOH
1.73


361
4-CF3-phenyl
O
H
N
O

B
—(CH2)2—COOH
1.45


363
2F,3Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.4


364
2F,4Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.44


365
2Cl,4F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.44


366
3Cl,5F-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.44


367
3F,4Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.45


369
2,3-diCl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.5


370
2Cl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.4


379
3,4-diCl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.5


380
3,5-diCl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.53


381
2,4-diCl-phenyl
CH2O
H
N
O

B
—(CH2)2—COOH
1.52


390
3,4-diF-phenyl
CH2O
3Cl
N
O

B
—CH(CH3)—CH2—COOH
1.56


392
3,4-diCl-phenyl
CH2O
3Cl
N
O

B
—CH(CH3)—CH2—COOH
1.67


393
2,3-diF-phenyl
CH2O
2Me
N
O

B
—CH(CH3)—(CH2)2—COOH
1.37


394
2,3-diF-phenyl
CH2O
2Me
N
O

B
—(CH2)2—CH(CH3)—COOH
1.38


395
4-Cl-phenyl
CH2O
2Me
N
O

B
—(CH2)2—CH(CH3)—COOH
1.43


396
4-CF3-phenyl
CH2O
2Me
N
O

B
—(CH2)2—CH(CH3)—COOH
1.47


397
3,4-diF-phenyl
CH2O
2Me
N
O

B
—(CH2)2—CH(CH3)—COOH
1.39


398
3,5-diF-phenyl
CH2O
2,6-dF
N
O

B
—(CH2)3—COOH
1.34


399
4CF3-phenyl
CH2O
2,6-dF
N
O

B
—(CH2)3—COOH
1.42


400
2,3-diF-phenyl
CH2O
H
N
O

B
—(CH2)2—CH(CH3)—COOH
#


401
Me
O
H
N
O

B
—(CH2)2—COOH
#)


402
Me
O
H
N
O

B
—(CH2)3—COOH
#


406
4Cl-phenyl
CH2O
2,3diF
N
O

B
—(CH2)3—COOH
1.37


407
3,5-diF-phenyl
CH2O
2,3diF
N
O

B
—(CH2)3—COOH
1.35


408
4Cl-phenyl
CH2O
2,3diF
N
O

B
—CH(CH3)—CH2—COOH
1.53


409
3,5-diF-phenyl
CH2O
2,3diF
N
O

B
—CH(CH3)—CH2—COOH
1.48


410
4Cl-phenyl
CH2O
2,3diF
N
O

B
trans cyclobutyl-COOH
1.41


411
3,5-diF-phenyl
CH2O
2,3diF
N
O

B
cis-cyclobutyl-COOH
1.41


412
3,5-diF-phenyl
CH2O
2,3diF
N
O

B
trans cyclobutyl-COOH
1.36


413
3,5-diF-phenyl
CH2O
2,3diF
N
O

B
cis-cyclobutyl-COOH
1.35


414
3,4-diF-phenyl
CH2O
2Me
N
O

B
cis-cyclobutyl-COOH
#


415
3,4-diF-phenyl
CH2O
2Me
N
O

B
trans cyclobutyl-COOH
#


416
2,3-diF-phenyl
CH2O
3F
N
O

B
—CH(CH3)—CH2—COOH
#


418
2,3-diF-phenyl
CH2O
3F
N
O

B
cis-cyclobutyl-COOH
#


419
3,5-diF-phenyl
CH2O
3Me
N
O

B
—(CH2)3—COOH
1.38


421
3,5-diF-phenyl
CH2O
3Me
N
O

B
—CH(CH3)—CH2—COOH
1.63


422
4CF3-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.12


423
3,4-diF-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.09


424
2,3-diF-phenyl
CH2O
3F
N
O

B
trans cyclobutyl-COOH
#


425
2,3-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH chiral2
1.11


426
2,3-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—CH2—COOH chiral1
1.2


427
3,5-diF-phenyl
CH2O
3Me
N
O

B
trans cyclobutyl-COOH
1.37


429
2,3-diF-phenyl
CH2O
3Me
N
O

B
trans cyclobutyl-COOH
1.35


431
3,5-diF-phenyl
CH2O
2Me
N
O

B
—CH(CH3)—CH2—COOH
1.5


434
2,3-diF-phenyl
CH2O
2Me
N
O

B
—CH(CH3)—CH2—COOH
1.47


437
2,3-diF-phenyl
CH2O
2Me
N
O

B
—CH2—CH(CH3)—CH2—COOH
1.42


439
2,3-diF-phenyl
CH2O
3Me
N
O

B
—CHCH3—CH2—COOH
1.49


441
4-Cl-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.38


442
2,3-diF-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.33


443
3,5-diF-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.34


444
3,4-diCl-phenyl
CH2S
H
N
O

B
—(CH2)3—COOH
1.44


445
4-Cl-phenyl
CH2O
H
N
S

B
—(CH2)3—COOH
1.36


446
3,5-diF-phenyl
CH2O
H
N
S

B
—(CH2)3—COOH
1.33


447
4-Cl-phenyl
CH2O
H
N
S

B
—CH(CH3)—CH2—COOH
1.53


448
3,5-diF-phenyl
CH2O
H
N
S

B
—CH(CH3)—CH2—COOH
1.48


451
3,5-diF-phenyl
C═C
H
N
O

B
—(CH2)3—COOH
1.39


452
3,5-diF-phenyl
(CH2)2
H
N
O

B
—(CH2)3—COOH
1.36


455
phenyl
cyclopropyl
3F
N
O

B
trans cyclobutyl-COOH
1.36


456
phenyl
cyclopropyl
3F
N
O

B
cis cyclobutyl-COOH
1.39


457
phenyl
cyclopropyl
3F
N
O

B
—CH(CH3)—CH2—COOH
1.55


459
phenyl
cyclopropyl
3F
N
O

B
trans cyclobutyl-COOH
1.36


460
phenyl
cyclopropyl
3F
N
O

B
cis cyclobutyl-COOH
1.39


461
phenyl
cyclopropyl
3F
N
O

B
—CH(CH3)—CH2—COOH
1.55


463
3,5-diF-phenyl
CH2O
H
N
O

B
—CH2COOH
1.51


464
3,5-diF-phenyl
CH2O
H
N
O

B
—CH(CH3)—COOH
1.53


465
3,5-diF-phenyl
CH2O
H
N
O

B
1,3-cyclopentyl-COOH
1.27





*= determined with LC-MS method B


# = NMR in accordance with proposed structure






§6. Pharmacological Tests & Data
In Vitro Functional Activity (Agonism) on Human S1P5 Receptors
The CHO-human-S1P5-Aeqorin assay was bought from Euroscreen, Brussels (Euroscreen, Technical dossier, Human Lysophospholid S1P5 (Edg8) receptor, DNA clone and CHO AequoScreenTM recombinant cell-line, catalog n°: ES-593-A, September 2006). Human-S1P5-Aequorin cells express mitochondrial targeted apo-Aequorin. Cells have to be loaded with coelanterazine, in order to reconstitute active Aequorin. After binding of agonists to the human S1P5 receptor the intracellular calcium concentration increases and binding of calcium to the apo-Aequorin/coelenterazine complex leads to an oxidation reaction of coelenterazine, which results in the production of apo-Aequorin, coelenteramide, CO2 and light (□max 469 nm). This luminescent response is dependent on the agonist concentration. Luminescence is measured using the MicroBeta Jet (Perkin Elmer). Agonistic effects of compounds are expressed as pEC50. Compounds were tested at a 10 points half log concentration range, and 3 independent experiments were performed in single point's measurements.
In Vitro Functional Activity (Agonism) on Human S1P3 Receptors
The CHO-human-S1P3-Aeqorin assay (CHO/Ga16/AEQ/h-S1P3) was established at Solvay Pharmaceuticals. The plasmid DNA coding for the S1P3 receptor (accession number in GenBank NM—005226 was purchased from UMR cDNA resource Centre (Rolla, Mo.). The pcDNA3.1/hS1P3 construct carrying the mitochondrially targeted apo-Aeqorin and Ga16 protein was transfected in CHO K1 cell-line. Human-S1P3-Aequorin cells express mitochondrial targeted apo-Aequorin. Cells have to be loaded with coelanterazine, in order to reconstitute active Aequorin. After binding of agonists to the human S1P3 receptor the intracellular calcium concentration increases and binding of calcium to the apo-Aequorin/coelenterazine complex leads to an oxidation reaction of coelenterazine, which results in the production of apo-Aequorin, coelenteramide, CO2 and light (□max 469 nm). This luminescent response is dependent on the agonist concentration. Luminescence is measured using the MicroBeta Jet (Perkin Elmer). Agonistic effects of compounds are expressed as pEC50. Compounds were tested at a 10 points half log concentration range, and 3 independent experiments were performed in single point's measurements.
In Vitro Functional Activity (Agonism) on Human S1P1 Receptors (Method A)
The CHO-K1-human-S1P1-Aeqorin assay was bought from Euroscreen Fast, Brussels (Euroscreen, Technical dossier, Human S1P1 (Edg1) receptor, DNA clone and CHO-K1 AequoScreenTM recombinant cell-line, catalog n°: FAST-0197L, February 2010). Human-S1P1-Aequorin cells express mitochondrial targeted apo-Aequorin. Cells have to be loaded with coelanterazine, in order to reconstitute active Aequorin. After binding of agonists to the human S1P1 receptor the intracellular calcium concentration increases and binding of calcium to the apo-Aequorin/coelenterazine complex leads to an oxidation reaction of coelenterazine, which results in the production of apo-Aequorin, coelenteramide, CO2 and light (□max 469 nm). This luminescent response is dependent on the agonist concentration. Luminescence is measured using the MicroBeta Jet (Perkin Elmer). Agonistic effects of compounds are expressed as pEC50. Compounds were tested at a 10 points half log concentration range, and 2 independent experiments were performed in single point's measurements.
In Vitro Functional Activity (Agonism) on Human S1P1 Receptors (Method B)
The CHO-K1-Human S1P1-c-AMP assay was performed at Euroscreenfast, Brussels (Euroscreen, Human S1P1 coupling Gi/0, (Edg1) receptor, catalog n°: FAST-0197C, December 2009).
Recombinant CHO-K1 cells expressing human S1P1, grown to mid-log Phase in culture media without antibiotics, detached, centrifuged and re-suspended. For agonist testing cells are mixed with compound and Forskolin and incubated at room temperature. Cells are lyses and cAMP concentration are estimated, according to the manufacturer specification, With the HTRF kit from CIS-BIO International (cat n°62AM2PEB).
Agonistic effects of compounds are expressed as a percentage of the activity of the reference compound at its EC100 concentration, EC50 is calculated and results are reported as pEC50. Compounds were tested at a 10 points half log concentration range duplicated in 1 experiment.
Pharmacological Data (Receptor Agonism) for Selected Compounds:















S1P5





Compound
pEC50
S1P1A pEC50
S1P1B pEC50
S1P3 pEC50



















 33
7.8

7.0
5.5


 35
8.3

6.6
5.3


 47
8.0

<5.5
<5


 53
7.9
<4.5

<5


 57
8.0

<5.5
<5


 73
8.2

8.0
6.3


 76
8.1

6.6
<5


 77
8.4

7.5
6.1


 85
8.6

<5.5
6.3


 89
8.2

5.9
<5.0


146
7.8

<5.5
<5.0


156
8.4

6.2
5.5


157
8.1

6.2
5.3


175
8.3

<5.5
5.8


211
8.3

6.1
<5.0


227
8.5

6.0
<6.0


271
8.0

6.2
5.4


277 (trans)
8.5
5.2

<5.0


278 (cis)
7.4
<5

<5


283
7.7
<4.5

<5


306 (trans)
8.1
<5

<5.0


307 (cis)
7.3
<5

<5.0





S1P1A: determined using method A


S1P1B: determined using method B






In Vivo Therapeutic Model; T-Maze
Age-related memory deficits occur in humans and rodents. Spontaneous alternation is the innate tendency of rodents to alternate free choices in a T-maze over a series of successive runs. This sequential procedure relies on working memory and is sensitive to various pharmacological manipulations affecting memory processes (Aging and the physiology of spatial memory. Barnes C. A. Neurobiol. Aging 1988:563-8; Dember W N, Fowler H. Spontaneous alternation behavior. Psychol. Bull. 1958, 55 (6):412-427; Gerlai R. A new continuous alternation task in T-maze detects hippocampal dysfunction in mice. A strain comparison and lesion study. Behav Brain Res 1998 95 (1):91-101).
For this study, male C57BL/6J mice of 2 months or 12 months old were used in the spontaneous alternation task in the T-maze. In short, mice were subjected to 1 session containing 15 trials, consisting of 1 “forced-choice” trial, followed by 14 “free-choice” trials. The animal was considered as entering one of the arms of the maze when all four paws are placed within this arm. A session is terminated and the animal is removed from the maze as soon as 14 free-choice trials have been performed or 15 min have elapsed, whatever event occurs first. The percentage of alternation over the 14 free-choice trials was determined for each mouse and was used as an index of working memory performance. A compound of the invention was administrated p.o. for 21 days prior the T-maze assay and on the day of the T-maze at t=−30 min. It was found that compounds of the invention at doses ranging from of 0.01-15 mg/kg/day reverse the age-related cognitive decline in the 12-month old C57BL6J mice with up to 100%. Thus, treated 12 month old mice were identical in their performance as 2 months old vehicle-treated mice. (See FIG. 1)
CONCLUSION
compounds of the present invention have a positive effect on age-related cognitive decline.

## Claims

1. A compound of the formula (I)



or a pharmaceutically acceptable salt, solvate, or hydrate thereof, or an N-oxide of any of the foregoing,
wherein
R1 is selected from the group consisting of:
a cyano group,
a group independently selected from a (2-4C)alkenyl group, a (2-4C)alkynyl group, and a (1-4C)alkyl group, wherein each group is optionally substituted with a substituent independently selected from CN and at least one halogen atom,
a group independently selected from a (3-6C)cycloalkyl group, a (4-6C)cycloalkenyl group, and a (8-10C)bicyclic group, wherein each group is optionally substituted with a substituent independently selected from a halogen atom and a (1-4C)alkyl group optionally substituted with at least one halogen atom, a group independently selected from a phenyl group, a biphenyl group, and a naphthyl group, wherein each group is optionally substituted with at least one substituent independently selected from halogen, cyano, (1-4C)alkyl optionally substituted with at least one halogen atom, (1-4C)alkoxy optionally substituted with at least one halogen atom, amino, dimethylamino, and (3-6C)cycloalkyl optionally substituted with a phenyl group wherein the phenyl group is optionally substituted with a substituent independently selected from (1-4C)alkyl and a halogen atom, and

a phenyl group substituted with a group selected from the group consisting of a phenoxy group, a benzyl group, a benzyloxy group, a phenylethyl group, and a monocyclic heterocycle group, wherein each group is optionally substituted with (1-4C)alkyl,

Z is a —W—(Cn-alkylene)-T-group wherein
W is attached to R1 and selected from the group consisting of a bond, —O—, —CO—, —S—, —SO—, —SO2—, —NH—, —CH═CH—, —C(CF3)═CH—, —C≡C—, —CH2—O—, —O—CO—, —CO—O—, —CO—NH—, NH—CO— and a trans-cyclopropylene;
n is an integer from 0 to 10; and
T is attached to the phenylene/pyridyl moiety and selected from the group consisting of a bond, —O—, —S—, —SO—, —SO2—, —NH—, —CO—, —C═C—, —C≡C—, and a trans-cyclopropylene;

R2 is selected from the group consisting of H and at least one substituent independently selected from cyano, halogen, (1-4C)alkyl optionally substituted with at least one halogen atom, and (1-4C)alkoxy optionally substituted with at least one or more halogen atom; ring structure A optionally contains a nitrogen atom;
X is selected from the group consisting of C and N;
wherein if X is C, R3 is selected from H and (1-4C)alkyl, and if X is N, R3 is not present;

Y is selected from the group consisting of NH, O and S;
structure Q is selected from the group consisting of a 5-, 6- and 7-membered cyclic amine; and
R4 is selected from
a (1-4C)alkylene-R5 group wherein at least one carbon atom in the alkylene group may independently be substituted with a substituent selected from at least one halogen atom and a (CH2)2 to form a cyclopropyl moiety, or
a group independently selected from (3-6C)cycloalkylene-R5, —CH2-(3-6C)cycloalkylene-R5, (3-6C)cycloalkylene-CH2—R5 and —CO—CH2—R5, wherein R5 is selected from —OH, —PO3H2, —OPO3H2, —COOH, —COO(1-4C)alkyl and tetrazol-5yl.

2. The compound of claim 1, wherein
R1 is selected from
a group independently selected from a (3-6C)cycloalkyl group and a (8-10C)bicyclic group wherein the group is optionally substituted with a halogen atom, (1-4C)alkyl, or a phenyl group wherein the phenyl group is optionally substituted with at least one substituent independently selected from the group consisting of a halogen atom, cyano, (1-4C)alkyl, (1-4C)alkoxy, a trifluoromethyl, and a trifluoromethoxy;

W is selected from the group consisting of a bond, —O—, —CO—, —S—, —SO—, —SO2—, —NH—, —CH═CH—, —C≡C—, and a trans-cyclopropylene;
n is an integer from 0 to 4; and
R2 is selected from the group consisting of H, at least one substituent independently selected from a halogen atom, (1-4C)alkyl optionally substituted with at least one fluoro and (1-4C)alkoxy optionally substituted with at least one fluoro atom.

3. The compound of claim 1, wherein the compound has the structure (Ia)



wherein R1, R2, R3, R4, R5, Q, T, W, X, Y, Z, and n are as defined in claim 1.

4. The compound of claim 1, wherein the compound has the structure (Ib)



wherein R1, R2, R3, R4, R5, Q, T, W, X, Y, Z, and n are as defined in claim 1.

5. The compound of claim 1, wherein Y is O.

6. The compound of claim 1, wherein R4 is selected from the group consisting of —(CH2)2—COOH, —(CH2)3—COOH, —CH2—CHCH3—COOH, —CH2—C(CH3)2—COOH, —CHCH3—CH2—COOH, —CH2—CF2—COOH, and 1,3-cyclobutylene-COOH.

7. The compound of claim 1, wherein R1 is a phenyl group optionally substituted with at least one substituent independently selected from the group consisting of halogen, cyano, (1-4C)alkyl optionally substituted with at least one halogen atom, (1-4C)alkoxy optionally substituted with at least one halogen atom, amino, dimethylamino, and (3-6C)cycloalkyl optionally substituted with a phenyl group wherein the phenyl group is optionally substituted with a substituent independently selected from (1-4C)alkyl and a halogen atom.

8. The compound of claim 7, wherein R1 is a phenyl group optionally substituted with at least one substituent independently selected from a halogen atom, cyano, (1-4C)alkyl, (1-4C)alkoxy, a trifluoromethyl, and a trifluoromethoxy.

9. The compound of claim 1, wherein Z is —CH2O—.

10. The compound of claim 1, wherein X is N.

11. The compound of claim 1, wherein the compound is selected from:
3-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-propionic acid,
3-{2-[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-2-methyl-propionic acid,
3-{2-[4-Benzyloxy-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid,
4-{2-[4-(2-Fluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid,
4-{2-[4-(2-Fluoro-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-4H-furo[3,2-c]pyridine-5-yl}-butyric acid,
3-{2-[4-(Benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-propionic acid, 4-{2-[4-(2,3-Difluoro-benzyloxy)-phenyl)]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-Benzyloxy-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-(4-Trifluoromethyl-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-(2-Fluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-(3,4-Dichloro-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-(2-Fluoro-benzyloxy)-3-chloro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
4-{2[4-(4-Chloro-benzyloxy)-3-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
3[2-(4-Benzyloxy-phenyl)-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl]-2-methyl-propionic acid,
3-{2[4-(4-Trifluoromethyl-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid,
3-{2[4-(4-Chloro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid,
3-{2[4-(2,3-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-butyric acid,
3-{2[4-(4-Trifluoromethyl-benzyloxy)-2-fluoro-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-2-methyl-propionic acid,
Cis and trans 3-{2[4-(4-Trifluoromethoxybenzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-cyclobutane carboxylic acid,
Cis and trans 3-{2[4-(3,4-Difluorobenzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-cyclobutane carboxylic acid,
3[2-(4-(2-Fluoro-benzyloxy)-phenyl)-6,7-dihydro-4H-thiazolo[5,4-c]pyridine-5-yl]-propionic acid,
4-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-thiazolo-[4,5c]-pyridin-5-yl}-butyric acid,
2-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-acetic acid,
2-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-H-oxazolo[4,5-c]pyridine-5-yl}-propionic acid,
3-{2-[4-(3,5-Difluoro-benzyloxy)-phenyl]-6,7-dihydro-4H-oxazolo[4,5-c]-pyridin-5-yl}-cyclopentane carboxylic acid,
4-{2-[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo[4,5c]-pyridin-5-yl}-pentanoic acid,
4-{2-[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo[4,5-c]-pyridin-5-yl}-2-methyl-butyric acid,
4-{2[4-(2,3-Difluoro-benzyloxy)-2-methyl-phenyl]-6,7-dihydro-4H-oxazolo-[4,5c]-pyridin-5-yl}-3-methyl-butyric acid,
3-{2-[4-(2-phenyl-cyclopropyl)-3-fluoro-phenyl]-6,7-dihydro-4H-oxazolo-[4,5-c]-pyridin-5-yl}-cyclobutane carboxylic acid,
4-(2-{4-(2-(3,5-Difluoro-phenyl)-vinyl]-phenyl}-6,7-dihydro-4H-oxazolo-[4,5c]-pyridin-5-yl)-butyric acid, and
4-(2-{4-[2-(3,5-Difluoro-phenyl)-ethyl]-phenyl}-6,7-dihydro-4H-oxazolo[4,5c]pyridin-5-yl)-butyric acid,
or a pharmaceutically acceptable salt, solvateor hydrate thereof, or an N-oxide of any of the foregoing.

12. A pharmaceutical composition comprising the compound of claim 1 and at least one pharmaceutically acceptable excipient.

13. A compound selected from the group consisting of:
3-(2-(4-((2-fluorobenzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)propanoic acid;
3-(2-(4-((4-chlorobenzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)-2-methylpropanoic acid;
2-methyl-3-(2-(4-((4-(trifluoromethyl)benzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)propanoic acid;
Trans 3-(2-(4-((3,4-difluorobenzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)cyclobutanecarboxylic acid;
3-(2-(4-((4-cyanobenzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)butanoic acid; and
3-(2-(4-((2,3-difluorobenzyl)oxy)phenyl)-6,7-dihydrooxazolo[4,5-c]pyridin-5(4H)-yl)butanoic acid,
or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

14. A compound comprising:



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

15. A compound comprising:



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

16. A compound comprising:



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

17. A compound comprising



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

18. A compound comprising:



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

19. A compound comprising



or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

20. A compound comprising the plus (+) enantiomer of




or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

21. A compound comprising the minus (−) enantiomer of




or a pharmaceutically acceptable salt, a solvate or hydrate thereof, or an N-oxide of any of the foregoing.

22. A pharmaceutical composition comprising a compound of claim 11, and at least one pharmaceutically acceptable excipient.

23. A pharmaceutical composition comprising a compound of claim 13, and at least one pharmaceutically acceptable excipient.

24. A pharmaceutical composition comprising a compound of claim 14, and at least one pharmaceutically acceptable excipient.

25. A pharmaceutical composition comprising a compound of claim 15, and at least one pharmaceutically acceptable excipient.

26. A pharmaceutical composition comprising a compound of claim 16, and at least one pharmaceutically acceptable excipient.

27. A pharmaceutical composition comprising a compound of claim 17, and at least one pharmaceutically acceptable excipient.

28. A pharmaceutical composition comprising a compound of claim 18, and at least one pharmaceutically acceptable excipient.

29. A pharmaceutical composition comprising a compound of claim 19, and at least one pharmaceutically acceptable excipient.

30. A pharmaceutical composition comprising a compound of claim 20, and at least one pharmaceutically acceptable excipient.

31. A pharmaceutical composition comprising a compound of claim 21, and at least one pharmaceutically acceptable excipient.