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## Overview

#### Senior Research Assistant

James Simone

simonej@mail.nih.gov

The Flow Cytometry Section provides state-of-the-art high-parameter cell analysis and sorting services to support NIAMS IRP investigators in their pursuit of the Institute’s mission. We provide sorting services, maintain and update the equipment, train instrument users, and collaborate on creative solutions to scientific questions. Our interests include single-cell multiomics, high-parameter data reduction analysis, and cytometric analysis of intracellular signaling events.

## Instrumentation

The facility currently houses a variety of state-of-the-art instrumentation for cell analysis and sorting.

### Cell Analysis

                          - BD FACSCanto RUO: 4-laser, 14-color analyzer with HTS (high throughput sampler)
                          - BD LSR Fortessa SORP: 7-laser, 18-color analyzer with HTS BD FACSCelesta: 4-laser, 15-color analyzer
                          - BD FACSymphony A5: 5-laser, 24-color analyzer with HTS
                          - Cytek Aurora: 5-laser, 35-color analyzer with autoloader
                          - BD FACSymphony A3: 5-laser, 24-color analyzer with HTS (Q3 2020)
                          - ThermoFisher Attune NxT: 4-laser, 14-color analyzer with autosampler (Q3 2020)

### Cell Sorting

                          - BD FACSAria IIIu: 5-laser, 18-color cell sorter with ACDU (automated cell deposition unit)
                          - BD FACSAria Fusion: 5-laser, 18-color cell sorter with ACDU and biosafety cabinet
                          - BD Influx: 7-laser, 18-color cell sorter with ACDU and biosafety cabinet
                          - BD FACSymphony S6: 5-laser, 30-color analyzer with ACDU and biosafety cabinet (Q1 2021)
                          - BD FACSMelody: 3-laser, 9-color cell sorter with biosafety cabinet (Q1 2021)

#### Contact Us

#### Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:
                                        - Biodata Mining and Discovery
                                        - Flow Cytometry
                                        - Genomic Technology
                                        - Laboratory Animal Care and Use
                                        - Light Imaging
                                        - Translational Immunology

## Staff

**James Simone**

simonej@mail.nih.gov

**Jeffrey T. Lay **

layj@mail.nih.gov 

**Kevin Tinsley**

tinsleykw@mail.nih.gov

## Image & Media Gallery

##### MoFlo Legacy

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## Scientific Publications

## Selected Recent Publications

###             FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle.        

(184).

###             Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells.        

25(8).

###             The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B.        

285(43).

###             The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis.        

114(21).

## News & Highlights

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