EXHIBIT
31.1


Certificate of Chief
Executive Officer


Rule 13a-14(a)/15d-14(a)
Certification of Chief Executive Officer and Chief Financial
Officer








I,
Douglas P. Baker, certify that:





1.           I
have reviewed this annual report on Form 10-K for the year ended December 31,
2009 of Applied Nanotech Holdings, Inc. (“APNT”);





2.           Based
on my knowledge, this report does not contain any untrue statement of a material
fact or omit to state a material fact necessary to make the statements made, in
light of the circumstances under which such statements were made, not misleading
with respect to the period covered by this report;





3.           Based
on my knowledge, the financial statements, and other financial information
included in this report, fairly present, in all material respects, the financial
condition, results of operations and cash flows of APNT as of, and for, the
periods presented in this report.





4.           APNT’s
other certifying officer and I are responsible for establishing and maintaining
disclosure controls and procedures (as defined in the Exchange Act Rules
13a-15(e) and 15d-15(e)) and internal control over financial reporting (as
defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) for APNT and
have:





(a)           Designed
such disclosure controls and procedures, or caused such disclosure controls and
procedures to be designed under our supervision, to ensure that material
information relating to APNT, including its consolidated subsidiaries, is made
known to us by others within those entities, particularly during the period in
which this report is being prepared;





(b)           Designed
such internal control over financial reporting, or caused such internal control
over financial reporting to be designed under our supervision, to provide
reasonable assurance regarding the reliability of financial reporting and the
preparation of financial statements for external purposes in accordance with
generally accepted accounting principals;





(c)           Evaluated
the effectiveness of APNT’s disclosure control’s and procedures and presented in
this report our conclusions about the effectiveness of the disclosure controls
and procedures, as of the end of the period covered by this report based on such
evaluation; and





(d)           Disclosed
in this report any change in APNT’s internal control over financial reporting
that occurred during APNT’s most recent fiscal quarter that has materially
affected, or is reasonably likely to materially affect, APNT’s internal control
over financial reporting; and





5.           APNT’s
other certifying officer and I have disclosed, based on our most recent
evaluation of internal control over financial reporting, to APNT’s auditors and
the audit committee of APNT’s board of directors:





(a)           All
significant deficiencies and material weaknesses in the design or operation of
internal control over financial reporting which are reasonably likely to
adversely affect APNT’s ability to record, process, summarize and report
financial information; and





(b)           Any
fraud, whether or not material, that involves management or other employees who
have a significant role in APNT’s internal control over financial
reporting.








Date:    March
4, 2010








/s/ Douglas P.
Baker


Douglas
P. Baker


Chief
Executive Officer and Chief Financial Officer