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          Labs @ NIAMS
          Office of Science and Technology
          Light Imaging Section








Light Imaging Section


Davide Randazzo, Ph.D. FacebookEmailPrintFacebookEmail
        lis hero image

      Overview

                                  Staff Scientist
      
  


Davide Randazzo, Ph.D.
      
  


            davide.randazzo@nih.gov
                                
            MissionThe principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. Furthermore, we use light microscopy and other techniques to elucidate poorly understood aspects of skeletal muscle cell biology, i.e. the organization of muscle microtubules and their role in muscle diseases. We also strive to apply new modalities of light microscopy to the quantitative analysis of skeletal muscle pathologies.ServicesThe Light Imaging Section is equipped for widefield and confocal microscopy, multi-photon microscopy, TIRF, high-throughput live cell imaging, and slide scanning.See full list of instruments available.For access to instruments and more information, contact Dr. Randazzo (davide.randazzo@nih.gov) or Ms. Kenea (aster.kenea@nih.gov).
      

                                            In the Lab with NIAMS
      
  

      Learn about the Light Imaging Section, a microscopy core with a variety of tools for image analysis.

                                                      Core Research Facilities

Labs at the NIAMS are supported by the following state-of-the-art facilities and services:

Biodata Mining and Discovery
	Flow Cytometry
	Genomic Technology
	Laboratory Animal Care and Use
	Light Imaging
	Translational Immunology

                                    Staff
                            
            **Davide Randazzo, Ph.D.**Staff Scientistdavide.randazzo@nih.gov
    **Aster Kenea, B.S.**Biologistaster.kenea@nih.gov
            
                                                Image & Media Gallery
              
            3D View of Human Monocytes
3D View of Human Monocytes
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      3D image of monoctyes
        This image was acquired using the Leica TCS SP8 X confocal scope, and applying a Z-stack acquisition modality to grab several focal planes along the Z-axis (to cover the entire volume of the cells). All focal planes were recomposed to create a 3D rendering of the cells and the image was exported and post-processed using the Imaris 9.7 image analysis software. The resulting image shows two monocytes, where nuclei (displaying the typical horseshoe shape) are depicted in violet and the plasma membrane in yellow with transparency.

Image acquired/processed by Robert Kwiat, Post-bac Fellow, Dr. Luis Franco’s Functional Immunogenomics Section, with assistance from Dr. Davide Randazzo, Chief, Light Imaging Section.
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                                    Scientific Publications
              Selected Recent Publications
                  
      Intravital imaging of muscle damage and response to therapy in a model of Pompe disease.

    Meena NK, Ng Y, Randazzo D, Weigert R, Puertollano R, Raben N
        

                    Clin Transl Med.
        
                    2024 Mar;
        
                    14(3).
        
                    doi: 10.1002/ctm2.1561
                            PMID: 38445455
              Intravital imaging of muscle damage and response to therapy in a model of Pompe disease.

    Meena NK, Ng Y, Randazzo D, Weigert R, Puertollano R, Raben N
        

                    Clin Transl Med.
        
                    2024 Mar;
        
                    14(3).
        
                    doi: 10.1002/ctm2.1561
                            PMID: 38445455
              Interrupting an IFN-γ-dependent feedback loop in the syndrome of pyogenic arthritis with pyoderma gangrenosum and acne.

    Lee W, Stone DL, Hoffmann P, Rosenzweig S, Tsai WL, Gadina M, Romeo T, Lee CR, Randazzo D, Pimpale Chavan P, Manthiram K, Canna S, Park YH, Ombrello AK, Aksentijevich I, Kastner DL, Chae JJ
        

                    Ann Rheum Dis.
        
                    2024 Feb 26;
        
        
                    pii: ard-2023-225085. doi: 10.1136/ard-2023-225085
                            PMID: 38408849
              Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 Rev.

    Eren E, Watts NR, Randazzo D, Palmer I, Sackett DL, Wingfield PT
        

                    Structure.
        
                    2023 Oct 5;
        
                    31(10).
        
                    doi: 10.1016/j.str.2023.07.009
                            PMID: 37572662
              Infigratinib, a selective FGFR1-3 tyrosine kinase inhibitor, alters dentoalveolar development at high doses.

    Michel ZD, Aitken SF, Glover OD, Alejandro LO, Randazzo D, Dambkowski C, Martin D, Collins MT, Somerman MJ, Chu EY
        

                    Dev Dyn.
        
                    2023 Dec;
        
                    252(12).
        
                    doi: 10.1002/dvdy.642
                            PMID: 37435833
              AAV-mediated delivery of secreted acid α-glucosidase with enhanced uptake corrects neuromuscular pathology in Pompe mice.

    Meena NK, Randazzo D, Raben N, Puertollano R
        

                    JCI Insight.
        
                    2023 Aug 22;
        
                    8(16).
        
                    doi: 10.1172/jci.insight.170199
                            PMID: 37463048
              Polycomb Ezh1 maintains murine muscle stem cell quiescence through non-canonical regulation of Notch signaling.

    Feng X, Wang AH, Juan AH, Ko KD, Jiang K, Riparini G, Ciuffoli V, Kaba A, Lopez C, Naz F, Jarnik M, Aliberti E, Hu S, Segalés J, Khateb M, Acevedo-Luna N, Randazzo D, Cheung TH, Muñoz-Cánoves P, Dell'Orso S, Sartorelli V
        

                    Dev Cell.
        
                    2023 Jun 19;
        
                    58(12).
        
                    doi: 10.1016/j.devcel.2023.04.005
                            PMID: 37105173
              Variant STAT4 and Response to Ruxolitinib in an Autoinflammatory Syndrome.

    Baghdassarian H, Blackstone SA, Clay OS, Philips R, Matthiasardottir B, Nehrebecky M, Hua VK, McVicar R, Liu Y, Tucker SM, Randazzo D, Deuitch N, Rosenzweig S, Mark A, Sasik R, Fisch KM, Pimpale Chavan P, Eren E, Watts NR, Ma CA, Gadina M, Schwartz DM, Sanyal A, Werner G, Murdock DR, Horita N, Chowdhury S, Dimmock D, Jepsen K, Remmers EF, Goldbach-Mansky R, Gahl WA, O'Shea JJ, Milner JD, Lewis NE, Chang J, Kastner DL, Torok K, Oda H, Putnam CD, Broderick L
        

                    N Engl J Med.
        
                    2023 Jun 15;
        
                    388(24).
        
                    doi: 10.1056/NEJMoa2202318
                            PMID: 37256972
              Influenza Defective Interfering Virus Promotes Multiciliated Cell Differentiation and Reduces the Inflammatory Response in Mice.

    Wang C, Honce R, Salvatore M, Chow D, Randazzo D, Yang J, Twells NM, Mahal LK, Schultz-Cherry S, Ghedin E
        

                    J Virol.
        
                    2023 Jun 29;
        
                    97(6).
        
                    doi: 10.1128/jvi.00493-23
                            PMID: 37255439
              Fostamatinib Inhibits Neutrophils Extracellular Traps Induced by COVID-19 Patient Plasma: A Potential Therapeutic.

    Strich JR, Ramos-Benitez MJ, Randazzo D, Stein SR, Babyak A, Davey RT, Suffredini AF, Childs RW, Chertow DS
        

                    J Infect Dis.
        
                    2021 Mar 29;
        
                    223(6).
        
                    doi: 10.1093/infdis/jiaa789
                            PMID: 33367731
              Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

    Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ
        

                    Ann Rheum Dis.
        
                    2021 Feb;
        
                    80(2).
        
                    doi: 10.1136/annrheumdis-2020-218338
                            PMID: 32988843
              A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils.

    Gupta S, Chan DW, Zaal KJ, Kaplan MJ
        

                    J Immunol.
        
                    2018 Jan 15;
        
                    200(2).
        
                    doi: 10.4049/jimmunol.1700905
                            PMID: 29196457
              Clearing skeletal muscle with CLARITY for light microscopy imaging.

    Milgroom A, Ralston E
        

                    Cell Biol Int.
        
                    2016 Apr;
        
                    40(4).
        
                    doi: 10.1002/cbin.10578
                            PMID: 26732743
              A new directionality tool for assessing microtubule pattern alterations.

    Liu W, Ralston E
        

                    Cytoskeleton (Hoboken).
        
                    2014 Apr;
        
                    71(4).
        
                    doi: 10.1002/cm.21166
                            PMID: 24497496
              Molecular editing of cellular responses by the high-affinity receptor for IgE.

    Suzuki R, Leach S, Liu W, Ralston E, Scheffel J, Zhang W, Lowell CA, Rivera J
        

                    Science.
        
                    2014 Feb 28;
        
                    343(6174).
        
                    doi: 10.1126/science.1246976
                            PMID: 24505132
              Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements.

    Oddoux S, Zaal KJ, Tate V, Kenea A, Nandkeolyar SA, Reid E, Liu W, Ralston E
        

                    J Cell Biol.
        
                    2013 Oct 28;
        
                    203(2).
        
                    doi: 10.1083/jcb.201304063
                            PMID: 24145165
              Quantitative evaluation of skeletal muscle defects in second harmonic generation images.

    Liu W, Raben N, Ralston E
        

                    J Biomed Opt.
        
                    2013 Feb;
        
                    18(2).
        
                    doi: 10.1117/1.JBO.18.2.026005
                            PMID: 23377006
            
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