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		Search Text: Home/< Back ToThe Science/Flow Cytometry Core
  Flow Cytometry Core
    The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting and analysis needs. This Core supports a wide variety of applications, including high speed cell sorting, 30-color cell analysis, cytokine analysis by multiplex bead arrays (Luminex), imaging flow cytometry (Amnis Image-Stream), phosphoprotein analysis, cell cycle and ploidy analysis, protocol standardization / methods development for high dimensional flow cytometric assays for longitudinal studies. Core also provides training for end users for instrument operation and assay specific training on one-on-one manner.
    On This Page
    
          generic avatar
  Pradeep K.  Dagur, Ph.D.
          Director and Staff Scientist
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      Meet the Team
            Contact us
  
  Research

            The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting, cell analysis and Immune monitoring needs. A wide variety of cytometry applications are supported from all types of samples originating from Human, Mouse, Rat, NHP, Drosophila, etc.**Assisted Service Facility (Bldg. 10 section: 8th floor, Room 8C104)****State-of-the-art aseptic sorting under BSL-2 conditions**1-to-6-way sorting of cells, nuclei, extracellular vesicles, chromosomes.Low-pressure, gentle sorting for cell cloning, iPSC, and genomics studies (Including single cell RNA Seq and 10X Genomics).High-speed bulk and single cell sorting for functional and genomics studies.**High parameter cell analyses (up to 30 colors)**Panel development and implementation for immunophenotyping studies.One-on-one personal assistance for methods development and standardizing protocols for sample preparation, tissue dissociation and deep immunophenotyping. We also support studies investigating apoptosis, cell cycle and ploidy, cell proliferation, drug screening, CRISPR screening/genotyping, and kinetics analysis, such as calcium flux.**State-of-the-art image cytometry (8 colors; up to 60X magnification)**Analyses of nuclear translocation, cellular colocalization, and cell-to-cell interactions.**Multiplexed quantitation of soluble analytes (Luminex platform)**Bead-based immune analyses of blood and body fluids, cell lysates, and culture supernatants.**Training and Consultation**One-on-one personal training for instrument operation, methods development, and troubleshooting.Teaching of best flow cytometry practices for data generation and analysis.Perform extended data analyses as needed.**24 x 7 Self-service Facility**24 x 7 operation at Bldg. 10 8th floor Corridor C (8C104), for all analyzers and at CRC 3rd floor (Rooms 3-5217, 3-5225, and 3-5209) for all analyzers and the Melody cell sorter by trained users.Assisted aseptic cell sorting, high parameter cell analyses (up to 15 colors), one-on-one assay specific panel design, methods development, training and teaching best practices of flow cytometry for data analysis services are also available on request using Melody cell sorter, FACS Aria-II sorter, and cell analyzers in CRC 24 x 7 section.**Cytometers**4-way, 5 Laser, 18 color FACS Aria II sorter4-way, 5 Laser, 18 color FACS Fusion sorter6-way, 6 Laser, 30 color FACS Fusion S6 sorter4-way, 5 Laser, 19 color FACS Aria II sorter4-way, 3 Laser, 9 color FACS Melody sorter7 Laser, 34 color FACS Symphony A5 Analyzer5 Laser, 20 color LSR Fortessa Analyzer3 Laser, 8 color FACS Canto II Analyzer2 Laser, 6 color FACS Canto I Analyzer5 Laser, 19 color Fortessa II Analyzer4 Laser, 17 color Fortessa I Analyzer5 Laser, 10 colors Amnis Imagestream Gen-X Mark II AnalyzerLuminex bead array AnalyzerCytospin
Featured Publications
                Systematic Analysis of Cell-to-Cell Expression Variation of T Lymphocytes in a Human Cohort Identifies Aging and Genetic Associations.
            Authors:Lu Y, Biancotto A, Cheung F, Remmers E, Shah N, McCoy JP, Tsang JS
                                                                                                                        
            Immunity
      
                2016 Nov 15;45(5):1162-1175
        Read Publication
                      High-density lipoprotein reduces inflammation from cholesterol crystals by inhibiting inflammasome activation.
            Authors:Thacker SG, Zarzour A, Chen Y, Alcicek MS, Freeman LA, Sviridov DO, Demosky SJ Jr, Remaley AT
                                                                                                                        
            Immunology
      
                2016 Nov 01;149(3):306-319
        Read Publication
                      Issue Highlights - September 2016.
            Authors:McCoy JP Jr
                                                                                                                        
            Cytometry B Clin Cytom
      
                2016 Sep 01;90(5):401-3
        Read Publication
                      Live Attenuated Leishmania donovani Centrin Knock Out Parasites Generate Non-inferior Protective Immune Response in Aged Mice against Visceral Leishmaniasis.
            Authors:Bhattacharya P, Dey R, Dagur PK, Joshi AB, Ismail N, Gannavaram S, Debrabant A, Akue AD, KuKuruga MA, Selvapandiyan A, McCoy JP Jr, Nakhasi HL
                                                                                                                        
            PLoS Negl Trop Dis
      
                2016 Aug 01;10(8):e0004963
        Read Publication
                      Masks in imaging flow cytometry.
            Authors:Dominical V, Samsel L, McCoy JP Jr
                                    
            Methods
      
                2016 Jul 25
        Read Publication
  Meet the Team

    generic avatar

            Pradeep K.  Dagur, Ph.D.
                Director and Staff Scientist
                Pradeep K. Dagur, Ph.D., is the director of the FlowCytometry****at the NHLBI.
    
          Contact the lab
    10 Center DriveBethesda, MD 20814United States
        Phone:
                    301-451-8824
                Fax:
            301-480-4774
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            Keyvan  Keyvanfar
        

    Section Chief
            generic avatar
    
            Ankit  Saxena, Ph.D.
        

    Staff Scientist

        ankit.saxena@nih.gov
            generic avatar
    
            Maria Lopez-Ocasio, Ph.D.
        

    Scientific Support Staff

        maria.ocasio@nih.gov
            generic avatar
    
            Steve Hockman
        

    Scientific Support Staff

        hockmans@nhlbi.nih.gov Back To Top Link
  

        

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