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						HOME
		Search Text: Home/< Back ToThe Science/Biochemistry Core
  Biochemistry Core
    The mission of the Biochemistry Core is to provide expert services and consultation in biochemical enzyme assays and protein purification.
    On This Page
    
          duck-yeon
  Duck-Yeon Lee, Ph.D.
          Core Director
          Email
      Meet the Team
            Contact us
  
  MissionThe Biochemistry Core provides biochemical analysis methods developed and implemented with a broad spectrum of LC/MS and HPLC based on the chemistry of protein/peptide/oligonucleotides/metabolites to separate and quantify analytes from complex biological samples. The Core is working at two locations, Mass spectrometry (Bldg. 50, Rm 3121); HPLC-based chromatography (Bldg. 10, Rm 8N117), and currently features 1) ESI-LC/MS systems and HPLC systems with UV/fluorescence detectors and 3) Atomic absorption spectrometer.Services**Accurate mass determination of intact protein and organic compounds**Using the LC/MS, we can get the accurate intact protein mass with an error < 1 Da per 50,000 Da. This service is important to users who are studying a purified protein. They can confirm the correct mass of a target molecule before moving to the next step.**Quantitation of specific metabolites**Using the HPLC or LC/MS, we can quantify physiological free amino acids, nucleotides, and other specific metabolites.**Elemental analysis in the biological sample**Using atomic absorption spectrometry, we can measure the metal content in solutions or protein samples. Currently, Zn, Mn, Fe, Ni, Cu, Al, and Se are available.**Consultation and development of customized chromatographic methods****On-site training for the system operation and data analysis**InstrumentsESI-LC/MS (Agilent 6530 QTOF and 6224 TOF)Agilent 1100 HPLC w/ DAD and FLD (fluorescence detector)Agilent 3100 OFFGel Fractionator Bio-Rad open column (low pressure) systemPerkin Elmer atomic absorption spectrometer (PinAAcle 900Z)
Featured Publications
                Humanin selectively prevents the activation of pro-apoptotic protein BID by sequestering it into fibers.
            Authors:Morris DL, Johnson S, Bleck CKE, Lee DY, Tjandra N.
                                                                                                                        
            J Biol Chem.
      
                2020 Dec 25;295(52):18226-18238
        Read Publication
                      Bax expression is optimal at low oxygen tension and constant agitation.
            Authors:He Y, Chen Y, Morris DL, Lee DY, Tjandra N.
                                                                                            
            Protein Expr Purif.
      
                2020 Jan 01;165:105501
        Read Publication
                      The ARH and Macrodomain Families of α-ADP-ribose-acceptor Hydrolases Catalyze α-NAD+ Hydrolysis.
            Authors:Stevens LA, Kato J, Kasamatsu A, Oda H, Lee DY, Moss J.
                                                                                                                        
            ACS Chem Biol.
      
                2019 Dec 20;14(12):2576-2584
        Read Publication
                      Humanin induces conformational changes in the apoptosis regulator BAX and sequesters it into fibers, preventing mitochondrial outer-membrane permeabilization.
            Authors:Morris DL, Kastner DW, Johnson S, Strub MP, He Y, Bleck CKE, Lee DY, Tjandra N.
                                                                                                                        
            J Biol Chem.
      
                2019 Dec 13;294(50):19055-19065
        Read Publication
                      MitoRACE: evaluating mitochondrial function in vivo and in single cells with subcellular resolution using multiphoton NADH autofluorescence.
            Authors:Willingham TB, Zhang Y, Andreoni A, Knutson JR, Lee DY, Glancy B.
                                                                                                                        
            J Physiol.
      
                2019 Nov 01;597(22):5411-5428
        Read Publication
  Meet the Team

    duck-yeon

            Duck-Yeon Lee, Ph.D.
                Core Director
                Duck-Yeon Lee, Ph.D., is the director of the Biochemistry Core Facility at the NHLBI.
    
          Contact the lab
    10 Center DrBethesda, MD 20814United States
        Phone:
                    301-435-8369
                Fax:
            301-451-5459 Back To Top Link
  

        

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