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						HOME
		Search Text: Home/< Back ToThe Science/Biophysics Core
  Biophysics Core
    The Biophysics Core's mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access.
    On This Page
    
          Grzegorz Piszczek
  Grzegorz Piszczek, Ph.D.
          Core Director
          Email
      Meet the Team
            Contact us
  
  Research

            The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. We assist researchers with comprehensive biophysical characterization of biomolecules, including proteins, polynucleotides, nanoparticles, and their cognate ligands.

The Biophysics Core has the resources and expertise to study oligomeric states of biomolecular assemblies and to perform measurements of affinity, stoichiometry, kinetics, and thermodynamics of interactions between proteins, DNA, RNA and small molecules. The Core facility is also equipped with instrumentation for the characterization of nanoparticles and macromolecules. Biophysical characterization capabilities include measurement of molecular weight, shape, and conformation of biological macromolecules.

Instruments

Isothermal Titration Calorimetry (ITC: iTC200 and VP-ITC)
	Differential Scanning Calorimetry (DSC: VP-DSC and Nano-DSC)
	Surface Plasmon Resonance (SPR: Biacore 3000)
	Bio-Layer Interferometry (BLI: Octet RED96)
	MicroScale Thermophoresis (MST: Monolith NT.115)
	Analytical Ultracentrifugation (AUC: XLA and XLI)
	Multi Angle Light Scattering (SEC-MALS/QELS: DAWN Helos II)
	Dynamic Light Scattering (DLS: Dynapro Nanostar)
	Circular Dichroism (CD: Jasco J715)
	Fluorescence (Photon Technology)
Featured Publications
                Olfactomedin 4 deletion induces colon adenocarcinoma in Apc(Min/+) mice.
            Authors:Liu W, Li H, Hong SH, Piszczek GP, Chen W, Rodgers GP
                                    
            Oncogene
      
                2016 Mar 14
        Read Publication
                      SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions.
            Authors:Zhao H, Piszczek G, Schuck P
                                                                                            
            Methods
      
                2015 Apr 01;76:137-48
        Read Publication
                      Fixation-resistant photoactivatable fluorescent proteins for CLEM.
            Authors:Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL
                                                                                                                        
            Nat Methods
      
                2015 Mar 01;12(3):215-8, 4 p following 218
        Read Publication
                      Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C.
            Authors:Gruschus JM, Jiang Z, Yap TL, Hill SA, Grishaev A, Piszczek G, Sidransky E, Lee JC
                                                                                                                        
            Biochem Biophys Res Commun
      
                2015 Feb 20;457(4):561-6
        Read Publication
                      A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.
            Authors:Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, Bakhtina MM, Becker DF, Bedwell GJ, Bekdemir A, Besong TM, Birck C, Brautigam CA, Brennerman W, Byron O, Bzowska A, Chaires JB, Chaton CT, Cölfen H, Connaghan KD, Crowley KA, Curth U, et. al
                                                                                                                        
            PLoS One
      
                2015 Jan 01;10(5):e0126420
        Read Publication
  Meet the Team

    Grzegorz Piszczek

            Grzegorz Piszczek, Ph.D.
                Core Director
                Grzegorz Piszczek, Ph.D., is the director of the Biophysics Core Facility at the NHLBI.
    
          Contact the lab
    10 Center DrBethesda, MD 20814United States
        Phone:
                    301-435-8082
                Fax:
            301-496-0599
  Di Wu
    
            Di Wu, Ph.D.
        

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