# Patent

## Title
Transgenic plants with increased stress tolerance and yield

## Abstract

Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

## Background
This application is a national stage application under 35 U.S.C. §371 of PCT/EP2008/059070, filed Jul. 11, 2008, which claims benefit of U.S. provisional application No. 60/959,346, filed Jul. 13, 2007. The entire contents of the above-identified applications are incorporated herein by reference.


FIELD OF THE INVENTION
This invention relates generally to transgenic plants which overexpress nucleic acid sequences encoding polypeptides capable of conferring increased stress tolerance and consequently, increased plant growth and crop yield, under normal or abiotic stress conditions. Additionally, the invention relates to novel isolated nucleic acid sequences encoding polypeptides that confer upon a plant increased tolerance under abiotic stress conditions, and/or increased plant growth and/or increased yield under normal or abiotic stress conditions.
BACKGROUND OF THE INVENTION
Abiotic environmental stresses, such as drought, salinity, heat, and cold, are major limiting factors of plant growth and crop yield. Crop yield is defined herein as the number of bushels of relevant agricultural product (such as grain, forage, or seed) harvested per acre. Crop losses and crop yield losses of major crops such as soybean, rice, maize (corn), cotton, and wheat caused by these stresses represent a significant economic and political factor and contribute to food shortages in many underdeveloped countries.
Water availability is an important aspect of the abiotic stresses and their effects on plant growth. Continuous exposure to drought conditions causes major alterations in the plant metabolism which ultimately lead to cell death and consequently to yield losses. Because high salt content in some soils results in less water being available for cell intake, high salt concentration has an effect on plants similar to the effect of drought on plants. Additionally, under freezing temperatures, plant cells lose water as a result of ice formation within the plant. Accordingly, crop damage from drought, heat, salinity, and cold stress, is predominantly due to dehydration.
Because plants are typically exposed to conditions of reduced water availability during their life cycle, most plants have evolved protective mechanisms against desiccation caused by abiotic stresses. However, if the severity and duration of desiccation conditions are too great, the effects on development, growth, plant size, and yield of most crop plants are profound. Developing plants efficient in water use is therefore a strategy that has the potential to significantly improve human life on a worldwide scale.
Traditional plant breeding strategies are relatively slow and require abiotic stress-tolerant founder lines for crossing with other germplasm to develop new abiotic stress-resistant lines. Limited germplasm resources for such founder lines and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Breeding for tolerance has been largely unsuccessful.
Many agricultural biotechnology companies have attempted to identify genes that could confer tolerance to abiotic stress responses, in an effort to develop transgenic abiotic stress-tolerant crop plants. Although some genes that are involved in stress responses or water use efficiency in plants have been characterized, the characterization and cloning of plant genes that confer stress tolerance and/or water use efficiency remains largely incomplete and fragmented. To date, success at developing transgenic abiotic stress-tolerant crop plants has been limited, and no such plants have been commercialized.
In order to develop transgenic abiotic stress-tolerant crop plants, it is necessary to assay a number of parameters in model plant systems, greenhouse studies of crop plants, and in field trials. For example, water use efficiency (WUE), is a parameter often correlated with drought tolerance. Studies of a plant's response to desiccation, osmotic shock, and temperature extremes are also employed to determine the plant's tolerance or resistance to abiotic stresses. When testing for the impact of the presence of a transgene on a plant's stress tolerance, the ability to standardize soil properties, temperature, water and nutrient availability and light intensity is an intrinsic advantage of greenhouse or plant growth chamber environments compared to the field.
WUE has been defined and measured in multiple ways. One approach is to calculate the ratio of whole plant dry weight, to the weight of water consumed by the plant throughout its life. Another variation is to use a shorter time interval when biomass accumulation and water use are measured. Yet another approach is to use measurements from restricted parts of the plant, for example, measuring only aerial growth and water use. WUE also has been defined as the ratio of CO2 uptake to water vapor loss from a leaf or portion of a leaf, often measured over a very short time period (e.g. seconds/minutes). The ratio of 13C/12C fixed in plant tissue, and measured with an isotope ratio mass-spectrometer, also has been used to estimate WUE in plants using C3 photosynthesis.
An increase in WUE is informative about the relatively improved efficiency of growth and water consumption, but this information taken alone does not indicate whether one of these two processes has changed or both have changed. In selecting traits for improving crops, an increase in WUE due to a decrease in water use, without a change in growth would have particular merit in an irrigated agricultural system where the water input costs were high. An increase in WUE driven mainly by an increase in growth without a corresponding jump in water use would have applicability to all agricultural systems. In many agricultural systems where water supply is not limiting, an increase in growth, even if it came at the expense of an increase in water use (i.e. no change in WUE), could also increase yield. Therefore, new methods to increase both WUE and biomass accumulation are required to improve agricultural productivity.
Concomitant with measurements of parameters that correlate with abiotic stress tolerance are measurements of parameters that indicate the potential impact of a transgene on crop yield. For forage crops like alfalfa, silage corn, and hay, the plant biomass correlates with the total yield. For grain crops, however, other parameters have been used to estimate yield, such as plant size, as measured by total plant dry weight, above-ground dry weight, above-ground fresh weight, leaf area, stem volume, plant height, rosette diameter, leaf length, root length, root mass, tiller number, and leaf number. Plant size at an early developmental stage will typically correlate with plant size later in development. A larger plant with a greater leaf area can typically absorb more light and carbon dioxide than a smaller plant and therefore will likely gain a greater weight during the same period. This is in addition to the potential continuation of the micro-environmental or genetic advantage that the plant had to achieve the larger size initially. There is a strong genetic component to plant size and growth rate, and so for a range of diverse genotypes plant size under one environmental condition is likely to correlate with size under another. In this way a standard environment is used to approximate the diverse and dynamic environments encountered at different locations and times by crops in the field.
Harvest index, the ratio of seed yield to above-ground dry weight, is relatively stable under many environmental conditions and so a robust correlation between plant size and grain yield is possible. Plant size and grain yield are intrinsically linked, because the majority of grain biomass is dependent on current or stored photosynthetic productivity by the leaves and stem of the plant. Therefore, selecting for plant size, even at early stages of development, has been used as to screen for plants that may demonstrate increased yield when exposed to field testing. As with abiotic stress tolerance, measurements of plant size in early development, under standardized conditions in a growth chamber or greenhouse, are standard practices to measure potential yield advantages conferred by the presence of a transgene.
There is a need, therefore, to identify additional genes expressed in stress tolerant plants and/or plants that are efficient in water use that have the capacity to confer stress tolerance and/or increased water use efficiency to the host plant and to other plant species. Newly generated stress tolerant plants and/or plants with increased water use efficiency will have many advantages, such as an increased range in which the crop plants can be cultivated, by for example, decreasing the water requirements of a plant species. Other desirable advantages include increased resistance to lodging, the bending of shoots or stems in response to wind, rain, pests, or disease.
SUMMARY OF THE INVENTION
The present inventors have discovered that transforming a plant with certain polynucleotides results in enhancement of the plant's growth and response to environmental stress, and accordingly the yield of the agricultural products of the plant is increased, when the polynucleotides are present in the plant as transgenes. The polynucleotides capable of mediating such enhancements have been isolated from Arabidopsis thaliana, Capsicum annuum, Escherichia coli, Physcomitrella patens, Saccharomyces cerevisiae, Triticum aestivum, Zea mays, Glycine max, Linum usitatissimum, Triticum aestivum, Oryza sativa, Helianthus annuus, and Brassica napus and the sequences thereof are set forth in the Sequence Listing as indicated in Table 1.










TABLE 1







Polynucleotide
Amino acid


Gene Name
Organism
SEQ ID NO
SEQ ID NO


















At2g20725
A. thaliana
1
2


At3g26085
A. thaliana
3
4


AtFACE-2
A. thaliana
5
6


ZM57353913
Z. mays
7
8


ZM59252659
Z. mays
9
10


CASAR82A
C. annuum
11
12


b3358
E. coli
13
14


EST564
P. patens
15
16


BN49502266
B. napus
17
18


GM49788080
G. max
19
20


GM53049821
G. max
21
22


ZM58462719
Z. mays
23
24


ZM61092633
Z. mays
25
26


ZM62016485
Z. mays
27
28


ZM62051019
Z. mays
29
30


ZM65086957
Z. mays
31
32


ZM68587657
Z. mays
33
34


EST390
P. patens
35
36


BN51363030
B. napus
37
38


BN42986056
B. napus
39
40


BN49389066
B. napus
41
42


BN51339479
B. napus
43
44


ZM57651070
Z. mays
45
46


ZM62073276
Z. mays
47
48


EST257
P. patens
49
50


LU61665952
L. usitatissimum
51
52


TA56863186
T. aestivum
53
54


ZM62026837
Z. mays
55
56


ZM65457595
Z. mays
57
58


ZM67230154
Z. mays
59
60


EST465
P. patens
61
62


YBL109w
S. cerevisiae
63
64


YBL100c
S. cerevisiae
65
66


YKL184w
S. cerevisiae
67
68


YPL091w
S. cerevisiae
69
70


TA54587433
T. aestivum
71
72


ZM68532504
Z. mays
73
74


BN42856089
B. napus
75
76


BN43206527
B. napus
77
78


HA66872964
H. annuus
79
80


LU61662612
L. usitatissimum
81
82


OS32806943
O. sativa
83
84


OS34738749
O. sativa
85
86


ZM59400933
Z. mays
87
88


ZM62132060
Z. mays
89
90


ZM59202533
Z. mays
91
92


BN41901422
B. napus
93
94


BN47868329
B. napus
95
96


BN42671700
B. napus
97
98


ZM68416988
Z. mays
99
100









In one embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 1.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 2.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a unknown protein 3.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a AP2 domain containing transcription factor.
In a further embodiment, the invention concerns a seed produced by the transgenic plant of the invention, wherein the seed is true breeding for a transgene comprising the polynucleotide described above. Plants derived from the seed of the invention demonstrate increased tolerance to an environmental stress, and/or increased plant growth, and/or increased yield, under normal or stress conditions as compared to a wild type variety of the plant.
In a still another aspect, the invention concerns products produced by or from the transgenic plants of the invention, their plant parts, or their seeds, such as a foodstuff, feedstuff, food supplement, feed supplement, cosmetic or pharmaceutical.
The invention further provides certain isolated polynucleotides identified in Table 1, and certain isolated polypeptides identified in Table 1. The invention is also embodied in recombinant vector comprising an isolated polynucleotide of the invention.
In yet another embodiment, the invention concerns a method of producing the aforesaid transgenic plant, wherein the method comprises transforming a plant cell with an expression vector comprising an isolated polynucleotide of the invention, and generating from the plant cell a transgenic plant that expresses the polypeptide encoded by the polynucleotide. Expression of the polypeptide in the plant results in increased tolerance to an environmental stress, and/or growth, and/or yield under normal and/or stress conditions as compared to a wild type variety of the plant.
In still another embodiment, the invention provides a method of increasing a plant's tolerance to an environmental stress, and/or growth, and/or yield. The method comprises the steps of transforming a plant cell with an expression cassette comprising an isolated polynucleotide of the invention, and generating a transgenic plant from the plant cell, wherein the transgenic plant comprises the polynucleotide.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows an alignment of the disclosed amino acid sequences AtFACE-2 (SEQ ID NO:6), ZM57353913 (SEQ ID NO:8), and ZM59252659 (SEQ ID NO:10). The alignment was generated using Align X of Vector NTI.
FIG. 2 shows an alignment of the disclosed amino acid sequences EST564 (SEQ ID NO:16), BN49502266 (SEQ ID NO:18), GM49788080 (SEQ ID NO:20), GM53049821 (SEQ ID NO:22), ZM58462719 (SEQ ID NO:24), ZM61092633 (SEQ ID NO:26), ZM62016485 (SEQ ID NO:28), ZM62051019 (SEQ ID NO:30), ZM65086957 (SEQ ID NO:32), and ZM68587657 (SEQ ID NO:34). The alignment was generated using Align X of Vector NTI.
FIG. 3 shows an alignment of the disclosed amino acid sequences EST390 (SEQ ID NO:36), BN51363030 (SEQ ID NO:38), BN42986056 (SEQ ID NO:40), BN49389066 (SEQ ID NO:42), BN51339479 (SEQ ID NO:44), ZM57651070 (SEQ ID NO:46), and ZM62073276 (SEQ ID NO:48). The alignment was generated using Align X of Vector NTI.
FIG. 4 shows an alignment of the disclosed amino acid sequences EST257 (SEQ ID NO:50), LU61665952 (SEQ ID NO:52), TA56863186 (SEQ ID NO:54), ZM62026837 (SEQ ID NO:56), ZM65457595 (SEQ ID NO:58), ZM67230154 (SEQ ID NO:60). The alignment was generated using Align X of Vector NTI.
FIG. 5 shows an alignment of the disclosed amino acid sequences ZM68532504 (SEQ ID NO:74), BN42856089 (SEQ ID NO:76), BN43206527 (SEQ ID NO:78), HA66872964 (SEQ ID NO:80), LU61662612 (SEQ ID NO:82), OS32806943 (SEQ ID NO:84), OS34738749 (SEQ ID NO:86), ZM59400933 (SEQ ID NO:88), and ZM62132060 (SEQ ID NO:90). The alignment was generated using Align X of Vector NTI.
FIG. 6 shows an alignment of the disclosed amino acid sequences ZM59202533 (SEQ ID NO:92), BN41901422 (SEQ ID NO:94), BN47868329 (SEQ ID NO:96), and ZM68416988 (SEQ ID NO:100). The alignment was generated using Align X of Vector NTI.



DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
Throughout this application, various publications are referenced. The disclosures of all of these publications and those references cited within those publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. The terminology used herein is for the purpose of describing specific embodiments only and is not intended to be limiting. As used herein, “a” or “an” can mean one or more, depending upon the context in which it is used. Thus, for example, reference to “a cell” can mean that at least one cell can be used.
In one embodiment, the invention provides a transgenic plant that overexpresses an isolated polynucleotide identified in Table 1, or a homolog thereof. The transgenic plant of the invention demonstrates an increased tolerance to an environmental stress as compared to a wild type variety of the plant. The overexpression of such isolated nucleic acids in the plant may optionally result in an increase in plant growth or in yield of associated agricultural products, under normal or stress conditions, as compared to a wild type variety of the plant.
As defined herein, a “transgenic plant” is a plant that has been altered using recombinant DNA technology to contain an isolated nucleic acid which would otherwise not be present in the plant. As used herein, the term “plant” includes a whole plant, plant cells, and plant parts. Plant parts include, but are not limited to, stems, roots, ovules, stamens, leaves, embryos, meristematic regions, callus tissue, gametophytes, sporophytes, pollen, microspores, and the like. The transgenic plant of the invention may be male sterile or male fertile, and may further include transgenes other than those that comprise the isolated polynucleotides described herein.
As used herein, the term “variety” refers to a group of plants within a species that share constant characteristics that separate them from the typical form and from other possible varieties within that species. While possessing at least one distinctive trait, a variety is also characterized by some variation between individuals within the variety, based primarily on the Mendelian segregation of traits among the progeny of succeeding generations. A variety is considered “true breeding” for a particular trait if it is genetically homozygous for that trait to the extent that, when the true-breeding variety is self-pollinated, a significant amount of independent segregation of the trait among the progeny is not observed. In the present invention, the trait arises from the transgenic expression of one or more isolated polynucleotides introduced into a plant variety. As also used herein, the term “wild type variety” refers to a group of plants that are analyzed for comparative purposes as a control plant, wherein the wild type variety plant is identical to the transgenic plant (plant transformed with an isolated polynucleotide in accordance with the invention) with the exception that the wild type variety plant has not been transformed with an isolated polynucleotide of the invention.
As defined herein, the term “nucleic acid” and “polynucleotide” are interchangeable and refer to RNA or DNA that is linear or branched, single or double stranded, or a hybrid thereof. The term also encompasses RNA/DNA hybrids. An “isolated” nucleic acid molecule is one that is substantially separated from other nucleic acid molecules which are present in the natural source of the nucleic acid (i.e., sequences encoding other polypeptides). For example, a cloned nucleic acid is considered isolated. A nucleic acid is also considered isolated if it has been altered by human intervention, or placed in a locus or location that is not its natural site, or if it is introduced into a cell by transformation. Moreover, an isolated nucleic acid molecule, such as a cDNA molecule, can be free from some of the other cellular material with which it is naturally associated, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. While it may optionally encompass untranslated sequence located at both the 3′ and 5′ ends of the coding region of a gene, it may be preferable to remove the sequences which naturally flank the coding region in its naturally occurring replicon.
As used herein, the term “environmental stress” refers to a sub-optimal condition associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, or oxidative stresses, or any combination thereof. The terms “water use efficiency” and “WUE” refer to the amount of organic matter produced by a plant divided by the amount of water used by the plant in producing it, i.e., the dry weight of a plant in relation to the plant's water use. As used herein, the term “dry weight” refers to everything in the plant other than water, and includes, for example, carbohydrates, proteins, oils, and mineral nutrients.
Any plant species may be transformed to create a transgenic plant in accordance with the invention. The transgenic plant of the invention may be a dicotyledonous plant or a monocotyledonous plant. For example and without limitation, transgenic plants of the invention may be derived from any of the following diclotyledonous plant families: Leguminosae, including plants such as pea, alfalfa and soybean; Umbelliferae, including plants such as carrot and celery; Solanaceae, including the plants such as tomato, potato, aubergine, tobacco, and pepper; Cruciferae, particularly the genus Brassica, which includes plant such as oilseed rape, beet, cabbage, cauliflower and broccoli); and A. thaliana; Compositae, which includes plants such as lettuce; Malvaceae, which includes cotton; Fabaceae, which includes plants such as peanut, and the like. Transgenic plants of the invention may be derived from monocotyledonous plants, such as, for example, wheat, barley, sorghum, millet, rye, triticale, maize, rice, oats and sugarcane. Transgenic plants of the invention are also embodied as trees such as apple, pear, quince, plum, cherry, peach, nectarine, apricot, papaya, mango, and other woody species including coniferous and deciduous trees such as poplar, pine, sequoia, cedar, oak, and the like. Especially preferred are Arabidopsis thaliana, Nicotiana tabacum, oilseed rape, soybean, corn (maize), wheat, linseed, potato and tagetes.
As shown in Table 1, one embodiment of the invention is a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a CAAX amino terminal protease family protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a CAAX amino terminal protease family protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a CAAX amino terminal protease family protein having a sequence comprising amino acids 1 to 301 of SEQ ID NO:2; and a protein having a sequence comprising amino acids 1 to 293 of SEQ ID NO:4.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a prenyl-dependent CAAX protease. The transgenic plant of this embodiment may comprise any polynucleotide encoding a prenyl-dependent CAAX protease. The transgenic plant of this embodiment comprises a polynucleotide encoding a prenyl-dependent CAAX protease having a sequence comprising amino acids 1 to 311 of SEQ ID NO:6; a protein having a sequence comprising amino acids 1 to 313 of SEQ ID NO:8; a protein having a sequence comprising amino acids 1 to 269 of SEQ ID NO:10.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a SAR8.2 protein precursor. The transgenic plant of this embodiment may comprise any polynucleotide encoding a SAR8.2 protein precursor. The transgenic plant of this embodiment comprises a polynucleotide encoding a SAR8.2 protein precursor having a sequence comprising amino acids 1 to 86 of SEQ ID NO:12.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a putative membrane protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a putative membrane protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a putative membrane protein having a sequence comprising amino acids 1 to 696 of SEQ ID NO:14.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2C protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2C protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2C protein having a sequence comprising amino acids 1 to 284 of SEQ ID NO:16; a protein having a sequence comprising amino acids 1 to 384 of SEQ ID NO:18; a protein having a sequence comprising amino acids 1 to 346 of SEQ ID NO:20; a protein having a sequence comprising amino acids 1 to 375 of SEQ ID NO:22; a protein having a sequence comprising amino acids 1 to 390 of SEQ ID NO:24; a protein having a sequence comprising amino acids 1 to 398 of SEQ ID NO:26; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:28; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:30; a protein having a sequence comprising amino acids 1 to 399 of SEQ ID NO:32; a protein having a sequence comprising amino acids 1 to 276 of SEQ ID NO:34.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial carrier protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a mitochondrial carrier protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a mitochondrial carrier protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:36; a protein having a sequence comprising amino acids 1 to 315 of SEQ ID NO:38; a protein having a sequence comprising amino acids 1 to 289 of SEQ ID NO:40; a protein having a sequence comprising amino acids 1 to 303 of SEQ ID NO:42; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:44; a protein having a sequence comprising amino acids 1 to 299 of SEQ ID NO:46; a protein having a sequence comprising amino acids 1 to 311 of SEQ ID NO:48.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein kinase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein kinase. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein kinase having a sequence comprising amino acids 1 to 356 of SEQ ID NO:50; a protein having a sequence comprising amino acids 1 to 364 of SEQ ID NO:52; a protein having a sequence comprising amino acids 1 to 361 of SEQ ID NO:54; a protein having a sequence comprising amino acids 1 to 370 of SEQ ID NO:56; a protein having a sequence comprising amino acids 1 to 377 of SEQ ID NO:58; a protein having a sequence comprising amino acids 1 to 382 of SEQ ID NO:60.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a peptidyl prolyl isomerase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a peptidyl prolyl isomerase. The transgenic plant of this embodiment comprises a polynucleotide encoding a peptidyl prolyl isomerase having a sequence comprising amino acids 1 to 523 of SEQ ID NO:62.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 1. The transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 1. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 1 having a sequence comprising amino acids 1 to 111 of SEQ ID NO:64.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 2. The transgenic plant of this embodiment may comprise any polynucleotide encoding an unknown protein 2. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 2 having a sequence comprising amino acids 1 to 104 of SEQ ID NO:66.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a ornithine decarboxylase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a ornithine decarboxylase. The transgenic plant of this embodiment comprises a polynucleotide encoding a ornithine decarboxylase having a sequence comprising amino acids 1 to 466 of SEQ ID NO:68.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a glutathione reductase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a glutathione reductase. The transgenic plant of this embodiment comprises a polynucleotide encoding a glutathione reductase having a sequence comprising amino acids 1 to 483 of SEQ ID NO:70.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an unknown protein 3. The transgenic plant of this embodiment may comprise any polynucleotide encoding a unknown protein 3. The transgenic plant of this embodiment comprises a polynucleotide encoding a unknown protein 3 having a sequence comprising amino acids 1 to 129 of SEQ ID NO:72.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a protein phosphatase 2A protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a protein phosphatase 2A protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a protein phosphatase 2A protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:74; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:76; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:78; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:80; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:82; a protein having a sequence comprising amino acids 1 to 307 of SEQ ID NO:84; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:86; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:88; a protein having a sequence comprising amino acids 1 to 306 of SEQ ID NO:90.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MEK1 protein kinase. The transgenic plant of this embodiment may comprise any polynucleotide encoding a MEK1 protein kinase. The transgenic plant of this embodiment comprises a polynucleotide encoding a MEK1 protein kinase having a sequence comprising amino acids 1 to 355 of SEQ ID NO:92; a protein having a sequence comprising amino acids 1 to 355 of SEQ ID NO:94; a protein having a sequence comprising amino acids 1 to 338 of SEQ ID NO:96; a protein having a sequence comprising amino acids 1 to 350 of SEQ ID NO:100.
In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an AP2 domain containing transcription factor. The transgenic plant of this embodiment may comprise any polynucleotide encoding a AP2 domain containing transcription factor. The transgenic plant of this embodiment comprises a polynucleotide encoding a AP2 domain containing transcription factor having a sequence comprising amino acids 1 to 197 of SEQ ID NO:98.
The invention further provides a seed produced by a transgenic plant expressing polynucleotide listed in Table 1, wherein the seed contains the polynucleotide, and wherein the plant is true breeding for increased growth and/or yield under normal or stress conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant. The invention also provides a product produced by or from the transgenic plants expressing the polynucleotide, their plant parts, or their seeds. The product can be obtained using various methods well known in the art. As used herein, the word “product” includes, but not limited to, a foodstuff, feedstuff, a food supplement, feed supplement, cosmetic or pharmaceutical. Foodstuffs are regarded as compositions used for nutrition or for supplementing nutrition. Animal feedstuffs and animal feed supplements, in particular, are regarded as foodstuffs. The invention further provides an agricultural product produced by any of the transgenic plants, plant parts, and plant seeds. Agricultural products include, but are not limited to, plant extracts, proteins, amino acids, carbohydrates, fats, oils, polymers, vitamins, and the like.
In a preferred embodiment, an isolated polynucleotide of the invention comprises a polynucleotide having a sequence selected from the group consisting of the polynucleotide sequences listed in Table 1. These polynucleotides may comprise sequences of the coding region, as well as 5′ untranslated sequences and 3′ untranslated sequences.
A polynucleotide of the invention can be isolated using standard molecular biology techniques and the sequence information provided herein, for example, using an automated DNA synthesizer.
“Homologs” are defined herein as two nucleic acids or polypeptides that have similar, or substantially identical, nucleotide or amino acid sequences, respectively. Homologs include allelic variants, analogs, and orthologs, as defined below. As used herein, the term “analogs” refers to two nucleic acids that have the same or similar function, but that have evolved separately in unrelated organisms. As used herein, the term “orthologs” refers to two nucleic acids from different species, but that have evolved from a common ancestral gene by speciation. The term homolog further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown in Table 1 due to degeneracy of the genetic code and thus encode the same polypeptide. As used herein, a “naturally occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural polypeptide).
To determine the percent sequence identity of two amino acid sequences (e.g., one of the polypeptide sequences of Table 1 and a homolog thereof), the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of one polypeptide for optimal alignment with the other polypeptide or nucleic acid). The amino acid residues at corresponding amino acid positions are then compared. When a position in one sequence is occupied by the same amino acid residue as the corresponding position in the other sequence then the molecules are identical at that position. The same type of comparison can be made between two nucleic acid sequences.
Preferably, the isolated amino acid homologs, analogs, and orthologs of the polypeptides of the present invention are at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99%, or more identical to an entire amino acid sequence identified in Table 1. In another preferred embodiment, an isolated nucleic acid homolog of the invention comprises a nucleotide sequence which is at least about 40-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and even more preferably at least about 95%, 96%, 97%, 98%, 99%, or more identical to a nucleotide sequence shown in Table 1.
For the purposes of the invention, the percent sequence identity between two nucleic acid or polypeptide sequences is determined using the Vector NTI 9.0 (PC) software package (Invitrogen, 1600 Faraday Ave., Carlsbad, Calif. 92008). A gap opening penalty of 15 and a gap extension penalty of 6.66 are used for determining the percent identity of two nucleic acids. A gap opening penalty of 10 and a gap extension penalty of 0.1 are used for determining the percent identity of two polypeptides. All other parameters are set at the default settings. For purposes of a multiple alignment (Clustal W algorithm), the gap opening penalty is 10, and the gap extension penalty is 0.05 with blosum62 matrix. It is to be understood that for the purposes of determining sequence identity when comparing a DNA sequence to an RNA sequence, a thymidine nucleotide is equivalent to a uracil nucleotide.
Nucleic acid molecules corresponding to homologs, analogs, and orthologs of the polypeptides listed in Table 1 can be isolated based on their identity to said polypeptides, using the polynucleotides encoding the respective polypeptides or primers based thereon, as hybridization probes according to standard hybridization techniques under stringent hybridization conditions. As used herein with regard to hybridization for DNA to a DNA blot, the term “stringent conditions” refers to hybridization overnight at 60° C. in 10×Denhart's solution, 6×SSC, 0.5% SDS, and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 62° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. As also used herein, in a preferred embodiment, the phrase “stringent conditions” refers to hybridization in a 6×SSC solution at 65° C. In another embodiment, “highly stringent conditions” refers to hybridization overnight at 65° C. in 10×Denhart's solution, 6×SSC, 0.5% SDS and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 65° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. Methods for performing nucleic acid hybridizations are well known in the art. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent or highly stringent conditions to a nucleotide sequence listed in Table 1 corresponds to a naturally occurring nucleic acid molecule.
There are a variety of methods that can be used to produce libraries of potential homologs from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene is then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential sequences. Methods for synthesizing degenerate oligonucleotides are known in the art.
Additionally, optimized nucleic acids can be created. Preferably, an optimized nucleic acid encodes a polypeptide that has a function similar to those of the polypeptides listed in Table 1 and/or modulates a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress, and more preferably increases a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress upon its overexpression in the plant. As used herein, “optimized” refers to a nucleic acid that is genetically engineered to increase its expression in a given plant or animal. To provide plant optimized nucleic acids, the DNA sequence of the gene can be modified to: 1) comprise codons preferred by highly expressed plant genes; 2) comprise an A+T content in nucleotide base composition to that substantially found in plants; 3) form a plant initiation sequence; 4) to eliminate sequences that cause destabilization, inappropriate polyadenylation, degradation and termination of RNA, or that form secondary structure hairpins or RNA splice sites; or 5) elimination of antisense open reading frames. Increased expression of nucleic acids in plants can be achieved by utilizing the distribution frequency of codon usage in plants in general or in a particular plant. Methods for optimizing nucleic acid expression in plants can be found in EPA 0359472; EPA 0385962; PCT Application No. WO 91/16432; U.S. Pat. No. 5,380,831; U.S. Pat. No. 5,436,391; Perlack et al., 1991, Proc. Natl. Acad. Sci. USA 88:3324-3328; and Murray et al., 1989, Nucleic Acids Res. 17:477-498.
An isolated polynucleotide of the invention can be optimized such that its distribution frequency of codon usage deviates, preferably, no more than 25% from that of highly expressed plant genes and, more preferably, no more than about 10%. In addition, consideration is given to the percentage G+C content of the degenerate third base (monocotyledons appear to favor G+C in this position, whereas dicotyledons do not). It is also recognized that the XCG (where X is A, T, C, or G) nucleotide is the least preferred codon in dicots, whereas the XTA codon is avoided in both monocots and dicots. Optimized nucleic acids of this invention also preferably have CG and TA doublet avoidance indices closely approximating those of the chosen host plant. More preferably, these indices deviate from that of the host by no more than about 10-15%.
The invention further provides an isolated recombinant expression vector comprising a polynucleotide as described above, wherein expression of the vector in a host cell results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the host cell. The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. As used herein with respect to a recombinant expression vector, “operatively linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in a bacterial or plant host cell when the vector is introduced into the host cell). The term “regulatory sequence” is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are well known in the art. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells and those that direct expression of the nucleotide sequence only in certain host cells or under certain conditions. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce polypeptides encoded by nucleic acids as described herein.
Plant gene expression should be operatively linked to an appropriate promoter conferring gene expression in a timely, cell specific, or tissue specific manner. Promoters useful in the expression cassettes of the invention include any promoter that is capable of initiating transcription in a plant cell. Such promoters include, but are not limited to, those that can be obtained from plants, plant viruses, and bacteria that contain genes that are expressed in plants, such as Agrobacterium and Rhizobium. 
The promoter may be constitutive, inducible, developmental stage-preferred, cell type-preferred, tissue-preferred, or organ-preferred. Constitutive promoters are active under most conditions. Examples of constitutive promoters include the CaMV 19S and 35S promoters, the sX CaMV 35S promoter, the Sep1 promoter, the rice actin promoter, the Arabidopsis actin promoter, the ubiquitan promoter, pEmu, the figwort mosaic virus 35S promoter, the Smas promoter, the super promoter (U.S. Pat. No. 5,955,646), the GRP1-8 promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), promoters from the T-DNA of Agrobacterium, such as mannopine synthase, nopaline synthase, and octopine synthase, the small subunit of ribulose biphosphate carboxylase (ssu-RUBISCO) promoter, and the like.
Inducible promoters are preferentially active under certain environmental conditions, such as the presence or absence of a nutrient or metabolite, heat or cold, light, pathogen attack, anaerobic conditions, and the like. For example, the hsp80 promoter from Brassica is induced by heat shock; the PPDK promoter is induced by light; the PR-1 promoters from tobacco, Arabidopsis, and maize are inducible by infection with a pathogen; and the Adh1 promoter is induced by hypoxia and cold stress. Plant gene expression can also be facilitated via an inducible promoter (For a review, see Gatz, 1997, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:89-108). Chemically inducible promoters are especially suitable if gene expression is wanted to occur in a time specific manner. Examples of such promoters are a salicylic acid inducible promoter (PCT Application No. WO 95/19443), a tetracycline inducible promoter (Gatz et al., 1992, Plant J. 2: 397-404), and an ethanol inducible promoter (PCT Application No. WO 93/21334).
In one preferred embodiment of the present invention, the inducible promoter is a stress-inducible promoter. For the purposes of the invention, stress-inducible promoters are preferentially active under one or more of the following stresses: sub-optimal conditions associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, and oxidative stresses. Stress inducible promoters include, but are not limited to, Cor78 (Chak et al., 2000, Planta 210:875-883; Hovath et al., 1993, Plant Physiol. 103:1047-1053), Cor15a (Artus et al., 1996, PNAS 93(23):13404-09), Rci2A (Medina et al., 2001, Plant Physiol. 125:1655-66; Nylander et al., 2001, Plant Mol. Biol. 45:341-52; Navarre and Goffeau, 2000, EMBO J. 19:2515-24; Capel et al., 1997, Plant Physiol. 115:569-76), Rd22 (Xiong et al., 2001, Plant Cell 13:2063-83; Abe et al., 1997, Plant Cell 9:1859-68; Iwasaki et al., 1995, Mol. Gen. Genet. 247:391-8), cDet6 (Lang and Palve, 1992, Plant Mol. Biol. 20:951-62), ADH1 (Hoeren et al., 1998, Genetics 149:479-90), KAT1 (Nakamura et al., 1995, Plant Physiol. 109:371-4), KST1 (Müller-Röber et al., 1995, EMBO 14:2409-16), Rha1 (Terryn et al., 1993, Plant Cell 5:1761-9; Terryn et al., 1992, FEBS Lett. 299(3):287-90), ARSK1 (Atkinson et al., 1997, GenBank Accession #L22302, and PCT Application No. WO 97/20057), PtxA (Plesch et al., GenBank Accession #X67427), SbHRGP3 (Ahn et al., 1996, Plant Cell 8:1477-90), GH3 (Liu et al., 1994, Plant Cell 6:645-57), the pathogen inducible PRP1-gene promoter (Ward et al., 1993, Plant. Mol. Biol. 22:361-366), the heat inducible hsp80-promoter from tomato (U.S. Pat. No. 5,187,267), cold inducible alpha-amylase promoter from potato (PCT Application No. WO 96/12814), or the wound-inducible pinII-promoter (European Patent No. 375091). For other examples of drought, cold, and salt-inducible promoters, such as the RD29A promoter, see Yamaguchi-Shinozalei et al., 1993, Mol. Gen. Genet. 236:331-340.
Developmental stage-preferred promoters are preferentially expressed at certain stages of development. Tissue and organ preferred promoters include those that are preferentially expressed in certain tissues or organs, such as leaves, roots, seeds, or xylem. Examples of tissue-preferred and organ-preferred promoters include, but are not limited to fruit-preferred, ovule-preferred, male tissue-preferred, seed-preferred, integument-preferred, tuber-preferred, stalk-preferred, pericarp-preferred, leaf-preferred, stigma-preferred, pollen-preferred, anther-preferred, petal-preferred, sepal-preferred, pedicel-preferred, silique-preferred, stem-preferred, root-preferred promoters, and the like. Seed-preferred promoters are preferentially expressed during seed development and/or germination. For example, seed-preferred promoters can be embryo-preferred, endosperm-preferred, and seed coat-preferred (See Thompson et al., 1989, BioEssays 10:108). Examples of seed-preferred promoters include, but are not limited to, cellulose synthase (celA), Cim1, gamma-zein, globulin-1, maize 19 kD zein (cZ19B1), and the like.
Other suitable tissue-preferred or organ-preferred promoters include the napin-gene promoter from rapeseed (U.S. Pat. No. 5,608,152), the USP-promoter from Vicia faba (Baeumlein et al., 1991, Mol. Gen. Genet. 225(3): 459-67), the oleosin-promoter from Arabidopsis (PCT Application No. WO 98/45461), the phaseolin-promoter from Phaseolus vulgaris (U.S. Pat. No. 5,504,200), the Bce4-promoter from Brassica (PCT Application No. WO 91/13980), or the legumin B4 promoter (LeB4; Baeumlein et al., 1992, Plant Journal, 2(2): 233-9), as well as promoters conferring seed specific expression in monocot plants like maize, barley, wheat, rye, rice, etc. Suitable promoters to note are the Ipt2 or Ipt1-gene promoter from barley (PCT Application No. WO 95/15389 and PCT Application No. WO 95/23230) or those described in PCT Application No. WO 99/16890 (promoters from the barley hordein-gene, rice glutelin gene, rice oryzin gene, rice prolamin gene, wheat gliadin gene, wheat glutelin gene, oat glutelin gene, Sorghum kasirin-gene, and rye secalin gene).
Other promoters useful in the expression cassettes of the invention include, but are not limited to, the major chlorophyll a/b binding protein promoter, histone promoters, the Ap3 promoter, the β-conglycin promoter, the napin promoter, the soybean lectin promoter, the maize 15 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the g-zein promoter, the waxy, shrunken 1, shrunken 2, and bronze promoters, the Zm13 promoter (U.S. Pat. No. 5,086,169), the maize polygalacturonase promoters (PG) (U.S. Pat. Nos. 5,412,085 and 5,545,546), and the SGB6 promoter (U.S. Pat. No. 5,470,359), as well as synthetic or other natural promoters.
Additional flexibility in controlling heterologous gene expression in plants may be obtained by using DNA binding domains and response elements from heterologous sources (i.e., DNA binding domains from non-plant sources). An example of such a heterologous DNA binding domain is the LexA DNA binding domain (Brent and Ptashne, 1985, Cell 43:729-736).
In a preferred embodiment of the present invention, the polynucleotides listed in Table 1 are expressed in plant cells from higher plants (e.g., the spermatophytes, such as crop plants). A polynucleotide may be “introduced” into a plant cell by any means, including transfection, transformation or transduction, electroporation, particle bombardment, agroinfection, and the like. Suitable methods for transforming or transfecting plant cells are disclosed, for example, using particle bombardment as set forth in U.S. Pat. Nos. 4,945,050; 5,036,006; 5,100,792; 5,302,523; 5,464,765; 5,120,657; 6,084,154; and the like. More preferably, the transgenic corn seed of the invention may be made using Agrobacterium transformation, as described in U.S. Pat. Nos. 5,591,616; 5,731,179; 5,981,840; 5,990,387; 6,162,965; 6,420,630, U.S. patent application publication number 2002/0104132, and the like. Transformation of soybean can be performed using for example a technique described in European Patent No. EP 0424047, U.S. Pat. No. 5,322,783, European Patent No. EP 0397 687, U.S. Pat. No. 5,376,543, or U.S. Pat. No. 5,169,770. A specific example of wheat transformation can be found in PCT Application No. WO 93/07256. Cotton may be transformed using methods disclosed in U.S. Pat. Nos. 5,004,863; 5,159,135; 5,846,797, and the like. Rice may be transformed using methods disclosed in U.S. Pat. Nos. 4,666,844; 5,350,688; 6,153,813; 6,333,449; 6,288,312; 6,365,807; 6,329,571, and the like. Other plant transformation methods are disclosed, for example, in U.S. Pat. Nos. 5,932,782; 6,153,811; 6,140,553; 5,969,213; 6,020,539, and the like. Any plant transformation method suitable for inserting a transgene into a particular plant may be used in accordance with the invention.
According to the present invention, the introduced polynucleotide may be maintained in the plant cell stably if it is incorporated into a non-chromosomal autonomous replicon or integrated into the plant chromosomes. Alternatively, the introduced polynucleotide may be present on an extra-chromosomal non-replicating vector and may be transiently expressed or transiently active.
Another aspect of the invention pertains to an isolated polypeptide having a sequence selected from the group consisting of the polypeptide sequences listed in Table 1. An “isolated” or “purified” polypeptide is free of some of the cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of a polypeptide in which the polypeptide is separated from some of the cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language “substantially free of cellular material” includes preparations of a polypeptide of the invention having less than about 30% (by dry weight) of contaminating polypeptides, more preferably less than about 20% of contaminating polypeptides, still more preferably less than about 10% of contaminating polypeptides, and most preferably less than about 5% contaminating polypeptides.
The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one skilled in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities are abundant and well known to one skilled in the art.
The invention is also embodied in a method of producing a transgenic plant comprising at least one polynucleotide listed in Table 1, wherein expression of the polynucleotide in the plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising the steps of: (a) introducing into a plant cell an expression vector comprising at least one polynucleotide listed in Table 1, and (b) generating from the plant cell a transgenic plant that expresses the polynucleotide, wherein expression of the polynucleotide in the transgenic plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the plant. The plant cell may be, but is not limited to, a protoplast, gamete producing cell, and a cell that regenerates into a whole plant. As used herein, the term “transgenic” refers to any plant, plant cell, callus, plant tissue, or plant part, that contains at least one recombinant polynucleotide listed in Table 1. In many cases, the recombinant polynucleotide is stably integrated into a chromosome or stable extra-chromosomal element, so that it is passed on to successive generations.
The present invention also provides a method of increasing a plant's growth and/or yield under normal or water-limited conditions and/or increasing a plant's tolerance to an environmental stress comprising the steps of increasing the expression of at least one polynucleotide listed in Table 1 in the plant. Expression of a protein can be increased by any method known to those of skill in the art.
The effect of the genetic modification on plant growth and/or yield and/or stress tolerance can be assessed by growing the modified plant under normal and or less than suitable conditions and then analyzing the growth characteristics and/or metabolism of the plant. Such analysis techniques are well known to one skilled in the art, and include dry weight, wet weight, polypeptide synthesis, carbohydrate synthesis, lipid synthesis, evapotranspiration rates, general plant and/or crop yield, flowering, reproduction, seed setting, root growth, respiration rates, photosynthesis rates, metabolite composition, etc., using methods known to those of skill in biotechnology.
The invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope thereof.
EXAMPLE 1
Cloning of cDNAs
cDNAs were isolated from proprietary libraries of the respective plant species using known methods. Sequences were processed and annotated using bioinformatics analyses. The degrees of amino acid identity and similarity of the isolated sequences to the respective closest known public sequences are indicated in Tables 2 through 18 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 2







Comparison of At2g20725 (SEQ ID NO: 2) to


known CAAX amino terminal protease proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_565483
A. thaliana
99.70%



ABE87113
Medicago truncatula
27.00%



NP_563943
A. thaliana
25.60%



AAU90306
Solanum tuberosum
25.20%



AAM65055
A. thaliana
25.00%

















TABLE 3







Comparison of At3g26085 (SEQ ID NO: 4) to


known CAAX amino terminal protease proteins











Public Database

Sequence



Accession #
Species
Identity (%)















NP_566788
A. thaliana
100.00%



BAC43478
A. thaliana
99.70%



AAM63917
A. thaliana
99.30%



NP_001078210
A. thaliana
91.00%



BAB01072
A. thaliana
65.30%

















TABLE 4







Comparison of AtFACE-2 (SEQ ID NO: 6) to


known prenyl-dependent CAAX proteases











Public Database

Sequence



Accession #
Species
Identity (%)















NP_850262
A. thaliana
100.00%



BAC43705
A. thaliana
99.70%



CAN61196
Vitis vinifera
36.70%



XP_695285
Danio rerio
32.70%



XP_001342272
D. rerio
32.70%

















TABLE 5







Comparison of CASAR82A (SEQ ID NO: 12)


to known SAR8.2 protein precursors











Public Database

Sequence



Accession #
Species
Identity (%)















AAF18935
C. annuum
100.00%



AAL56986
C. annuum
97.70%



AAL16783
C. annuum
93.00%



AAL16782
C. annuum
91.90%



AAR97871
C. annuum
52.30%

















TABLE 6







Comparison of b3358 (SEQ ID NO: 14)


to known putative membrane proteins











Public Database

Sequence



Accession #
Species
Identity (%)







YP_312284
Shigella sonnei
99.90%



ZP_00715046
E. coli
99.90%



ZP_00725390
E. coli
99.60%



AP_004431
E. coli
99.40%



YP_858957
E. coli
99.40%

















TABLE 7







Comparison of EST564 (SEQ ID NO: 16) to


known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







ABF93864
O. sativa
56.40%



NP_974411
A. thaliana
51.60%



AAC35951
Mesembryanthemum
51.10%




crystallinum



EAZ25504
O. sativa
45.70%



EAZ02383
O. sativa
43.40%

















TABLE 8







Comparison of EST390 (SEQ ID NO: 36) to


known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_172866
A. thaliana
63.50%



AAT66766
Solanum demissum
60.80%



CAH67091
O. sativa
60.00%



CAE01569
O. sativa
59.70%



CAN75338
V. vinifera
59.50%

















TABLE 9







Comparison of EST257 (SEQ ID NO: 50) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001043682
O. sativa
62.20%



CAN82019
V. vinifera
62.10%



AAR01726
O. sativa
61.10%



NP_001056408
O. sativa
61.10%



CAN64754
V. vinifera
60.90%

















TABLE 10







Comparison of EST465 (SEQ ID NO: 62)


to known peptidyl prolyl isomerases











Public Database

Sequence



Accession #
Species
Identity (%)







AAC39445
A. thaliana
54.30%



ABE85899
M. truncatula
54.20%



CAB88363
A. thaliana
54.10%



NP_566993
A. thaliana
53.80%



NP_001050182
O. sativa
53.00%

















TABLE 11







Comparison of YBL109w (SEQ ID NO: 64) to unknown protein 1











Public Database

Sequence



Accession #
Species
Identity (%)







CAA84936
S. cerevisiae
49.50%



P38898
S. cerevisiae
43.10%

















TABLE 12







Comparison of YBL100c (SEQ ID NO: 66) to unknown protein 2











Public Database

Sequence



Accession #
Species
Identity (%)







P38168
S. cerevisiae
100.00%

















TABLE 13







Comparison of YKL184w (SEQ ID NO: 68)


to known ornithine decarboxylases











Public Database

Sequence



Accession #
Species
Identity (%)















NP_012737
S. cerevisiae
100.00%



XP_445434
Candida glabrata
70.90%



XP_451651
Kluyveromyces lactis
60.30%



NP_984947
Ashbya gossypii
57.40%



XP_001385782
P. stipitis
49.80%

















TABLE 14







Comparison of YPL091w (SEQ ID NO:


70) to known glutathione reductases











Public Database

Sequence



Accession #
Species
Identity (%)















NP_015234
S. cerevisiae
100.00%



AAA92575
S. cerevisiae
96.70%



BAA07109
S. cerevisiae
95.70%



XP_447042
C. glabrata
79.90%



XP_455036
K. lactis
73.30%

















TABLE 15







Comparison of TA54587433 (SEQ


ID NO: 72) to unknown protein 3











Public Database

Sequence



Accession #
Species
Identity (%)







EAY88696
O. sativa
22.80%



EAZ25723
O. sativa
21.90%



NP_001049087
O. sativa
21.20%

















TABLE 16







Comparison of ZM68532504 (SEQ ID NO: 74)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAC72838
O. sativa
95.40%



AAA91806
O. sativa
94.10%



BAA92697
Vicia faba
93.10%



AAQ67226
Lycopersicon esculentum
92.80%



BAD17175
O. sativa
92.80%

















TABLE 17







Comparison of ZM59202533 (SEQ ID NO:


92) to known MEK1 protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)















AAC83393
Z. mays
100.00%



ABG45894
O. sativa
92.70%



NP_001043164
O. sativa
85.90%



BAB32405
Nicotiana tabacum
77.80%



CAC24705
N. tabacum
77.20%

















TABLE 18







Comparison of BN42671700 (SEQ ID NO: 98) to known


AP2 domain containing transcription factors











Public Database

Sequence



Accession #
Species
Identity (%)







NP_177631
A. thaliana
58.60%



NP_173355
A. thaliana
56.70%



AAF82238
A. thaliana
54.80%










The full-length DNA sequence of the AtFACE-2 (SEQ ID NO: 5) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 19 and 20 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 19







Comparison of ZM57353913 (SEQ ID NO: 8)


to known prenyl-dependent CAAX proteases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_850262
A. thaliana
52.20%



BAC43705
A. thaliana
52.20%



NP_001055298
O. sativa
42.10%



EAZ33973
O. sativa
36.60%



XP_001353747
Drosophila pseudoobscura
33.50%

















TABLE 20







Comparison of ZM59252659 (SEQ ID NO: 10)


to known prenyl-dependent CAAX proteases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_850262
A. thaliana
47.00%



BAC43705
A. thaliana
47.00%



EAZ33973
O. sativa
41.10%



NP_001055298
O. sativa
38.30%



CAN61196
V. vinifera
31.90%










The full-length DNA sequence of EST564 (SEQ ID NO: 15) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Six homologs from maize, two homologs from soybean, and one homolog from canola were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 21-29 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 21







Comparison of BN49502266 (SEQ ID NO: 18)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_195118
A. thaliana
91.10%



NP_001067133
O. sativa
63.20%



EAY83661
O. sativa
60.80%



EAZ21008
O. sativa
60.50%



CAN76780
V. vinifera
57.60%

















TABLE 22







Comparison of GM49788080 (SEQ ID NO: 20)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







EAZ02383
O. sativa
75.60%



EAZ38299
O. sativa
75.30%



CAB90634
Fagus sylvatica
73.80%



EAZ25504
O. sativa
73.00%



AAC35951
M. crystallinum
72.80%

















TABLE 23







Comparison of GM53049821 (SEQ ID NO: 22)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







CAN72598
V. vinifera
82.40%



NP_566566
A. thaliana
73.50%



AAM61747
A. thaliana
73.50%



BAA94987
A. thaliana
73.00%



NP_001051801
O. sativa
60.20%

















TABLE 24







Comparison of ZM58462719 (SEQ ID NO: 24)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001058597
O. sativa
91.10%



EAZ02383
O. sativa
81.20%



EAZ38299
O. sativa
81.00%



AAD11430
M. crystallinum
75.70%



CAB90634
F. sylvatica
74.20%

















TABLE 25







Comparison of ZM61092633 (SEQ ID NO: 26)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001065203
O. sativa
87.00%



AAK20060
O. sativa
86.00%



NP_001048899
O. sativa
80.70%



EAY88457
O. sativa
79.90%



ABE77197
Sorghum bicolor
77.20%

















TABLE 26







Comparison of ZM62016485 (SEQ ID NO: 28)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







ABE77197
S. bicolor
90.70%



NP_001048899
O. sativa
86.20%



EAY88457
O. sativa
85.20%



NP_001065203
O. sativa
78.50%



AAK20060
O. sativa
77.80%

















TABLE 27







Comparison of ZM62051019 (SEQ ID NO: 30)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







ABE77197
S. bicolor
92.50%



NP_001048899
O. sativa
88.00%



EAY88457
O. sativa
87.00%



NP_001065203
O. sativa
79.50%



AAK20060
O. sativa
78.80%

















TABLE 28







Comparison of ZM65086957 (SEQ ID NO: 32)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







ABE77197
S. bicolor
91.00%



NP_001048899
O. sativa
86.50%



EAY88457
O. sativa
85.50%



NP_001065203
O. sativa
78.80%



AAK20060
O. sativa
78.00%

















TABLE 29







Comparison of ZM68587657 (SEQ ID NO: 34)


to known protein phosphatase 2C proteins











Public Database

Sequence



Accession #
Species
Identity (%)







EAZ02383
O. sativa
70.60%



EAZ38299
O. sativa
70.60%



AAC35951
M. crystallinum
69.80%



ABF93864
O. sativa
68.50%



NP_974411
A. thaliana
65.00%










The full-length DNA sequence of the EST390 (SEQ ID NO: 35) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Four homologs from canola and two homologs from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 30-35 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 30







Comparison of BN51363030 (SEQ ID NO: 38)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







CAN77545
V. vinifera
71.90%



BAE71294
Trifolium pratense
71.90%



NP_194188
A. thaliana
70.70%



AAU11466
Saccharum officinarum
70.60%



AAU11465
S. officinarum
69.90%

















TABLE 31







Comparison of BN42986056 (SEQ ID NO: 40)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_179836
A. thaliana
74.80%



AAK44155
A. thaliana
74.50%



AAM63236
A. thaliana
74.20%



CAN77545
V. vinifera
67.70%



BAE71294
Trifolium pratense
65.50%

















TABLE 32







Comparison of BN49389066 (SEQ ID NO: 42)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAY97866
L. esculentum
73.50%



CAA68164
Solanum tuberosum
73.50%



CAC84547
N. tabacum
73.30%



AAR06239
Citrus junos
73.00%



CAC84545
N. tabacum
73.00%

















TABLE 33







Comparison of BN51339479 (SEQ ID NO: 44)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







CAC84545
N. tabacum
85.60%



CAC84547
N. tabacum
85.30%



AAR06239
C. junos
85.30%



CAA68164
S. tuberosum
85.30%



CAC12820
N. tabacum
85.30%

















TABLE 34







Comparison of ZM57651070 (SEQ ID NO: 46)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001066927
O. sativa
57.00%



NP_680566
A. thaliana
53.80%



BAF00711
A. thaliana
51.70%



CAN71674
V. vinifera
43.20%



CAN71674
V. vinifera
43.20%

















TABLE 35







Comparison of ZM62073276 (SEQ ID NO: 48)


to known mitochondrial carrier proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAU11471
S. officinarum
94.90%



NP_001054904
O. sativa
92.30%



BAA08105
Panicum miliaceum
86.20%



BAA08103
P. miliaceum
85.50%



EAY80779
O. sativa
82.90%










The full-length DNA sequence of the EST257 (SEQ ID NO: 49) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Three homologs from maize, one homolog from linseed, and one sequence from wheat were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 36-40 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 36







Comparison of LU61665952 (SEQ ID


NO: 52) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_566716
A. thaliana
75.10%



CAN82019
V. vinifera
74.50%



NP_193214
A. thaliana
74.50%



ABK06452
synthetic construct
73.00%



ABK06453
synthetic construct
72.30%

















TABLE 37







Comparison of TA56863186 (SEQ ID


NO: 54) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







AAO72550
O. sativa
87.30%



NP_001046047
O. sativa
79.80%



EAZ01979
O. sativa
73.80%



NP_001058291
O. sativa
73.60%



AAO48744
O. sativa
73.40%

















TABLE 38







Comparison of ZM62026837 (SEQ ID


NO: 56) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







AAR01726
O. sativa
83.40%



NP_001050732
O. sativa
77.00%



EAY91142
O. sativa
76.30%



EAZ27891
O. sativa
76.00%



CAN82019
V. vinifera
73.30%

















TABLE 39







Comparison of ZM65457595 (SEQ ID


NO: 58) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001056408
O. sativa
89.60%



AAO72572
O. sativa
87.20%



NP_001043682
O. sativa
81.50%



CAN64754
V. vinifera
79.80%



NP_199811
A. thaliana
77.20%

















TABLE 40







Comparison of ZM67230154 (SEQ ID


NO: 60) to known protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_001043682
O. sativa
87.10%



NP_001056408
O. sativa
82.80%



AAO72572
O. sativa
80.80%



EAZ12861
O. sativa
79.20%



CAN64754
V. vinifera
77.50%










The full-length DNA sequence of the ZM68532504 (SEQ ID NO: 73) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola, two homologs from maize, one homolog from linseed, two sequences from rice and one sequence from sunflower were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 41-48 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 41







Comparison of BN42856089 (SEQ ID NO: 76)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_172514
A. thaliana
97.10%



AAM65099
A. thaliana
95.80%



AAQ67226
L. esculentum
95.40%



BAA92697
Vicia faba
95.10%



CAC11129
Fagus sylvatica
94.40%

















TABLE 42







Comparison of BN43206527 (SEQ ID NO: 78)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







NP_172514
A. thaliana
97.40%



AAM65099
A. thaliana
96.10%



AAQ67226
L. esculentum
95.10%



BAA92697
V. faba
94.10%



AAQ67225
L. esculentum
94.10%

















TABLE 43







Comparison of HA66872964 (SEQ ID NO: 80)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







P48579
H. annuus
99.30%



BAA92697
V. faba
93.50%



CAC11129
F. sylvatica
93.10%



BAA92698
V. faba
92.80%



Q9ZSE4
Hevea brasiliensis
92.80%

















TABLE 44







Comparison of LU61662612 (SEQ ID NO: 82)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAQ67226
L. esculentum
94.10%



BAA92697
V. faba
94.10%



BAA92698
V. faba
94.10%



CAN74947
V. vinifera
93.50%



CAC11129
F. sylvatica
93.10%

















TABLE 45







Comparison of OS32806943 (SEQ ID NO: 84)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAC72838
O. sativa
96.10%



BAD17175
O. sativa
95.80%



AAA91806
O. sativa
94.80%



AAQ67226
L. esculentum
93.20%



BAA92697
V. faba
93.20%

















TABLE 46







Comparison of OS34738749 (SEQ ID NO: 86)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAQ67226
L. esculentum
97.70%



BAA92697
V. faba
97.10%



CAC11129
F. sylvatica
96.70%



BAA92698
V. faba
96.10%



AAQ67225
L. esculentum
96.10%

















TABLE 47







Comparison of ZM59400933 (SEQ ID NO: 88)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAC72838
O. sativa
95.80%



AAA91806
O. sativa
94.40%



BAA92697
V. faba
92.80%



AAQ67226
L. esculentum
92.80%



AAQ67225
L. esculentum
92.80%

















TABLE 48







Comparison of ZM62132060 (SEQ ID NO: 90)


to known protein phosphatase 2A proteins











Public Database

Sequence



Accession #
Species
Identity (%)







AAC72838
O. sativa
95.10%



AAA91806
O. sativa
93.80%



BAA92697
V. faba
92.80%



AAQ67226
L. esculentum
92.50%



BAD17175
O. sativa
92.50%










The full-length DNA sequence of the ZM59202533 (SEQ ID NO: 91) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, sunflower, and wheat cDNAs at an e value of e−10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola and one homolog from maize were identified. The degree of amino acid identity of these sequences to the closest known public sequences is indicated in Tables 49-51 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum62).







TABLE 49







Comparison of BN41901422 (SEQ ID NO:


94) to known MEK1 protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







ABF55661
synthetic construct
79.80%



NP_849446
A. thaliana
76.30%



AAQ96337
Vitis aestivalis
66.00%



AAL62336
G. max
64.10%



AAS21304
Petroselinum crispum
63.60%

















TABLE 50







Comparison of BN47868329 (SEQ ID NO:


96) to known MEK1 protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







NP_188759
A. thaliana
72.30%



CAA68958
A. thaliana
72.00%



ABF55664
synthetic construct
70.90%



AAL91161
A. thaliana
70.10%



AAU04434
L. esculentum
66.40%

















TABLE 51







Comparison of ZM68416988 (SEQ ID NO:


100) to known MEK1 protein kinases











Public Database

Sequence



Accession #
Species
Identity (%)







ABI93775
Oryza minuta
80.00%



NP_001056806
O. sativa
79.70%



ABP88102
O. sativa
78.90%



CAD45180
O. sativa
75.20%



ABI93776
O. minuta
72.40%










EXAMPLE 2
Well-Watered Arabidopsis Plants
The polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker. The resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter. The recombinant vectors are transformed into an Agrobacterium tumefaciens strain according to standard conditions. A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions. T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene. T3 seeds are used in greenhouse or growth chamber experiments.
Approximately 3-5 days prior to planting, seeds are refrigerated for stratification. Seeds are then planted, fertilizer is applied and humidity is maintained using transparent domes. Plants are grown in a greenhouse at 22° C. with photoperiod of 16 hours light/8 hours dark. Plants are watered twice a week.
At 19 and 22 days, plant area, leaf area, biomass, color distribution, color intensity, and growth rate for each plant are measured using a commercially available imaging system. Biomass is calculated as the total plant leaf area at the last measuring time point. Growth rate is calculated as the plant leaf area at the last measuring time point minus the plant leaf area at the first measuring time point divided by the plant leaf area at the first measuring time point. Health index is calculated as the dark green leaf area divided by the total plant leaf area.
EXAMPLE 3
Nitrogen Stress Tolerant Arabidopsis Plants
The polynucleotides of Table 1 are ligated into a binary vector containing a selectable marker. The resulting recombinant vector contains the corresponding gene in the sense orientation under a constitutive promoter. The recombinant vectors are transformed into an A. tumefaciens strain according to standard conditions. A. thaliana ecotype Col-0 or C24 are grown and transformed according to standard conditions. T1 and T2 plants are screened for resistance to the selection agent conferred by the selectable marker gene.
Plants are grown in flats using a substrate that contains no organic components. Each flat is wet with water before seedlings resistant to the selection agent are transplanted onto substrate. Plants are grown in a growth chamber set to 22° C. with a 55% relative humidity with photoperiod set at 16 h light/8 h dark. A controlled low or high nitrogen nutrient solution is added to waterings on Days 12, 15, 22 and 29. Watering without nutrient solution occurs on Days 18, 25, and 32. Images of all plants in a tray are taken on days 26, 30, and 33 using a commercially available imaging system. At each imaging time point, biomass and plant phenotypes for each plant are measured including plant area, leaf area, biomass, color distribution, color intensity, and growth rate.
EXAMPLE 4
Stress-Tolerant Rapeseed/Canola Plants
Canola cotyledonary petioles of 4 day-old young seedlings are used as explants for tissue culture and transformed according to EP1566443. The commercial cultivar Westar (Agriculture Canada) is the standard variety used for transformation, but other varieties can be used. A. tumefaciens GV3101:pMP90RK containing a binary vector is used for canola transformation. The standard binary vector used for transformation is pSUN (WO02/00900), but many different binary vector systems have been described for plant transformation (e.g. An, G. in Agrobacterium Protocols, Methods in Molecular Biology vol 44, pp 47-62, Gartland K M A and M R Davey eds. Humana Press, Totowa, N.J.). A plant gene expression cassette comprising a selection marker gene, a plant promoter, and a polynucleotide of Table 1 is employed. Various selection marker genes can be used including the mutated acetohydroxy acid synthase (AHAS) gene disclosed in U.S. Pat. Nos. 5,767,366 and 6,225,105. A suitable promoter is used to regulate the trait gene to provide constitutive, developmental, tissue or environmental regulation of gene transcription.
Canola seeds are surface-sterilized in 70% ethanol for 2 min, incubated for 15 min in 55° C. warm tap water and then in 1.5% sodium hypochlorite for 10 minutes, followed by three rinses with sterilized distilled water. Seeds are then placed on MS medium without hormones, containing Gamborg B5 vitamins, 3% sucrose, and 0.8% Oxoidagar. Seeds are germinated at 24° C. for 4 days in low light (<50 μMol/m2s, 16 hours light). The cotyledon petiole explants with the cotyledon attached are excised from the in vitro seedlings, and inoculated with Agrobacterium by dipping the cut end of the petiole explant into the bacterial suspension. The explants are then cultured for 3 days on MS medium including vitamins containing 3.75 mg/l BAP, 3% sucrose, 0.5 g/l MES, pH 5.2, 0.5 mg/l GA3, 0.8% Oxoidagar at 24° C., 16 hours of light. After three days of co-cultivation with Agrobacterium, the petiole explants are transferred to regeneration medium containing 3.75 mg/l BAP, 0.5 mg/l GA3, 0.5 g/l MES, pH 5.2, 300 mg/l timentin and selection agent until shoot regeneration. As soon as explants start to develop shoots, they are transferred to shoot elongation medium (A6, containing full strength MS medium including vitamins, 2% sucrose, 0.5% Oxoidagar, 100 mg/l myo-inositol, 40 mg/l adenine sulfate, 0.5 g/l MES, pH 5.8, 0.0025 mg/l BAP, 0.1 mg/l IBA, 300 mg/l timentin and selection agent).
Samples from both in vitro and greenhouse material of the primary transgenic plants (T0) are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations.
Seed is produced from the primary transgenic plants by self-pollination. The second-generation plants are grown in greenhouse conditions and self-pollinated. The plants are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations. Homozygous transgenic, heterozygous transgenic and azygous (null transgenic) plants are compared for their stress tolerance, for example, in the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
EXAMPLE 5
Screening for Stress-Tolerant Rice Plants
Transgenic rice plants comprising a polynucleotide of Table 1 are generated using known methods. Approximately 15 to 20 independent transformants (T0) are generated. The primary transformants are transferred from tissue culture chambers to a greenhouse for growing and harvest of T1 seeds. Five events of the T1 progeny segregated 3:1 for presence/absence of the transgene are retained. For each of these events, 10 T1 seedlings containing the transgene (hetero- and homozygotes), and 10 T1 seedlings lacking the transgene (nullizygotes) are selected by visual marker screening. The selected T1 plants are transferred to a greenhouse. Each plant receives a unique barcode label to link unambiguously the phenotyping data to the corresponding plant. The selected T1 plants are grown on soil in 10 cm diameter pots under the following environmental settings: photoperiod=11.5 h, daylight intensity=30,000 lux or more, daytime temperature=28° C. or higher, night time temperature=22° C., relative humidity=60-70%. Transgenic plants and the corresponding nullizygotes are grown side-by-side at random positions. From the stage of sowing until the stage of maturity, the plants are passed several times through a digital imaging cabinet. At each time point digital, images (2048×1536 pixels, 16 million colours) of each plant are taken from at least 6 different angles.
The data obtained in the first experiment with T1 plants are confirmed in a second experiment with T2 plants. Lines that have the correct expression pattern are selected for further analysis. Seed batches from the positive plants (both hetero- and homozygotes) in T1 are screened by monitoring marker expression. For each chosen event, the heterozygote seed batches are then retained for T2 evaluation. Within each seed batch, an equal number of positive and negative plants are grown in the greenhouse for evaluation.
Transgenic plants are screened for their improved growth and/or yield and/or stress tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
EXAMPLE 6
Stress-Tolerant Soybean Plants
The polynucleotides of Table 1 are transformed into soybean using the methods described in commonly owned copending international application number WO 2005/121345, the contents of which are incorporated herein by reference.
The transgenic plants generated are then screened for their improved growth under water-limited conditions and/or drought, salt, and/or cold tolerance, for example, using the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
EXAMPLE 7
Stress-Tolerant Wheat Plants
The polynucleotides of Table 1 are transformed into wheat using the method described by Ishida et al., 1996, Nature Biotech. 14745-50. Immature embryos are co-cultivated with Agrobacterium tumefaciens that carry “super binary” vectors, and transgenic plants are recovered through organogenesis. This procedure provides a transformation efficiency between 2.5% and 20%. The transgenic plants are then screened for their improved growth and/or yield under water-limited conditions and/or stress tolerance, for example, is the assays described in Examples 2 and 3, and for yield, both in the greenhouse and in field studies.
EXAMPLE 8
Stress-Tolerant Corn Plants
The polynucleotides of Table 1 are transformed into immature embryos of corn using Agrobacterium. After imbibition, embryos are transferred to medium without selection agent. Seven to ten days later, embryos are transferred to medium containing selection agent and grown for 4 weeks (two 2-week transfers) to obtain transformed callus cells. Plant regeneration is initiated by transferring resistant calli to medium supplemented with selection agent and grown under light at 25-27° C. for two to three weeks. Regenerated shoots are then transferred to rooting box with medium containing selection agent. Plantlets with roots are transferred to potting mixture in small pots in the greenhouse and after acclimatization are then transplanted to larger pots and maintained in greenhouse till maturity.
Using assays such as the assay described in Examples 2 and 3, each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. Trans-gene positive and negative plants are marked and paired with similar sizes for transplanting together to large pots. This provides a uniform and competitive environment for the trans-gene positive and negative plants. The large pots are watered to a certain percentage of the field water capacity of the soil depending the severity of water-stress desired. The soil water level is maintained by watering every other day. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. After a period of growth, the above ground portion of the plants is harvested, and the fresh weight and dry weight of each plant are taken. A comparison of the drought tolerance phenotype between the transgene positive and negative plants is then made.
Using assays such as the assay described in Example 2 and 3, the pots are covered with caps that permit the seedlings to grow through but minimize water loss. Each pot is weighed periodically and water added to maintain the initial water content. At the end of the experiment, the fresh and dry weight of each plant is measured, the water consumed by each plant is calculated and WUE of each plant is computed. Plant growth and physiology traits such as WUE, height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the experiment. A comparison of WUE phenotype between the transgene positive and negative plants is then made.
Using assays such as the assay described in Example 2 and 3, these pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for trans-gene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Water is then withheld. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured as stress intensity increases. A comparison of the dessication tolerance phenotype between transgene positive and negative plants is then made.
Segregating transgenic corn seeds for a transformation event are planted in small pots for testing in a cycling drought assay. These pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Plants are then repeatedly watered to saturation at a fixed interval of time. This water/drought cycle is repeated for the duration of the experiment. Plant growth and physiology traits such as height, stem diameter, leaf rolling, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. At the end of the experiment, the plants are harvested for above-ground fresh and dry weight. A comparison of the cycling drought tolerance phenotype between transgene positive and negative plants is then made.
In order to test segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated “rain-out shelter” which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Each plot is sown with seed segregating for the presence of a single transgenic insertion event. A Taqman transgene copy number assay is used on leaf samples to differentiate the transgenics from null-segregant control plants. Plants that have been genotyped in this manner are also scored for a range of phenotypes related to drought-tolerance, growth and yield. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plants are used as the replicate unit per event.
In order to test non-segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated “rain-out shelter” which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A null segregant is progeny (or lines derived from the progeny) of a transgenic plant that does not contain the transgene due to Mendelian segregation. Additional replicated paired plots for a particular event are distributed around the trial. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.
To perform multi-location testing of transgenic corn for drought tolerance and yield, five to twenty locations encompassing major corn growing regions are selected. These are widely distributed to provide a range of expected crop water availabilities based on average temperature, humidity, precipitation and soil type. Crop water availability is not modified beyond standard agronomic practices. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes included plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO2 uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.
APPENDIX
cDNA sequence of At2g20725 from Arabidopsis (SEQ ID NO:1):







ATGATTCTAGGCCGATGGGTTTCCTTCAGTTGCGGAAACACGCCGGTGAC


 


TAATTGTTCCGAACGACGACGACATACGGAGTTTCGTCGTCTCTCCTCTG


 


CTAGTACTTGTCGACCTTCTCTGATATGCTCTTGTCTCAAAAGCAAATCC


 


TCCCAAGAAACTACTCAGATTGAACAGTTGGGGAATGGAGAAGGGTTCTC


 


AGTTTTGGCATCAGAGATTCCATGGGAGGATGATAACATATGGAGCACAT


 


TTGCTCTTTACATGTTCTCTCTGCATATTCCTCTCAGTTTTGGGGGTTTA


 


TCCATTGTTGCCAACATACTCCACCGGCAGGTTCTTGATCCTCAGACCCA


 


AGTGCTATCACTTGTGGTTCTCCAAATGGTAGAACTTGCAGGGACAGTCT


 


TGCTGCTGAGGACCACAGCGAAGCCTCAGTGCAAATCAATCAACTTTCTA


 


AAGGGTAATAACGAAACAAGGGAAGGAAGAAACTGTGTGGTTGGCTCAGC


 


ATTGGGTTTGGGATGTCTTGTGGGCTTTATCTTCGTCACGTCGCTTGTAG


 


CTGATCAACTCTTTGGCCCTAAGGCTGTACATGAATCAGAATTGGAGAAG


 


ATAATGGTGAGGCGGGAAGTGGCGAGAAGCGGATGTTTTGCTCTCTACTG


 


CGTAGTAGCTCCCATCCTTGAGGAGATAGTGTACAGACGCTTTCTCCTGA


 


CCTCCTTAGCGTCGAGAATGGAATGGTGGAAGGCACTAGTGATCAGCTCA


 


GGAGTATTTGCTGCAGGTCACTTCTCAGGTGAGGATTTTGTGCAGCTGTT


 


TGGGATAGGTTGCGGTCTCGGGTTATGTTACAGCTGGTCAGGGAACTTAG


 


CCTCATCAGTGCTCGTCCACTCCTTGTACAATGCATTGACACTTCTCTTC


 


TCTTAG






The At2g20725 cDNA is translated into the following amino acid sequence (SEQ ID NO:2):








MILGRWVSFSCGNTPVTNCSERRRHTEFRRLSSASTCRPSLICSCLKSKS


 


SQETTQIEQLGNGEGFSVLASEIPWEDDNIWSTFALYMFSLHIPLSFGGL


 


SIVANILHRQVLDPQTQVLSLVVLQMVELAGTVLLLRTTAKPQCKSINFL


 


KGNNETREGRNCVVGSALGLGCLVGFIFVTSLVADQLFGPKAVHESELEK


 


IMVSGEVARSGCFALYCVVAPILEEIVYRRFLLTSLASRMEWWKALVISS


 


GVFAAGHESGEDEVQLFGIGCGLGLCYSWSGNLASSVLVHSLYNALTLLF


 


S






cDNA sequence of At3g26085 from Arabidopsis (SEQ ID NO:3):








ATGGGTTCCATCGCTCTGCAATCTTGGAGCAGAGGAGCTTCAACTTCTCT


 


TCATCTCCTTTTTCGTCCAGTTGGCTCGAACCCTAAGCTATATGATGCTC


 


GAAGAGTACAATTTGATGTAAGAGCCTCTTCAAGTCGTAAATCACTTAAG


 


AAACTCAAAAGAGAGTCACAACAAGGTAAAGACATAGGCTTAAGAAATGT


 


TACAGATGAAGAAGTTTCTTCTCCAAGATTTGAGGAAGCTCAAGTTGATT


 


CTTCAACTTCAAAGGACTCCATTGATGTTTTTGTTGCTGCTCCTCGAGAC


 


AAAGTGCTTCAGGCTTGCACTGTAACTTCCGGTTTGATGGCTGCACTAGG


 


TCTGATCATCAGAAAGGCGTCTCATGTTGCTTCGACTGAAGGATTACTGG


 


TTCCAGACTGCTCTATAGATGTACCATTTGGGTTTGAAACTTGGCATCTC


 


GGTTTAATTGCTGGAATCGTTGTGTTTATATCGTCAAGTAGGTTCTTGCT


 


ACTTAAATCTTGGCCAGATTTTGCTGATTCTAGTGAAGCAGCAAACCGAC


 


AGATTCTCACTTCCCTCGAACCTCTAGATTACCTTGTTGTTGCAATGTTA


 


CCGGGAATAAGTGAGGAGCTGCTGTTTAGAGGTGCATTAATGCCTTTGTT


 


CGGAACTAATTGGAATGGTATTGTAGCGGTTGGCCTCATTTTCGGTTTAC


 


TTCATCTCGGGAGCGGAAGAAAGTATTCTTTTGCAGTTTGGGCTTCGATT


 


GTCGGTATAGTCTACGGTTATGCAGCTGTTTTGTCGTCGAGTCTTATCGT


 


TCCAATGGCCTCGCACGCACTCAACAATTTGGTGGGAGGTCTGTTGTGGC


 


GATATAGTTCCAAGATCAAGTCATTGGAG-TAA






The At3g26085 cDNA is translated into the following amino acid sequence (SEQ ID NO:4):








MGSIALQSWSRGASTSLHLLFRPVGSNPKLYDARRVQFDVRASSSRKSLK


 


KLKRESQQGKDIGLRNVTDEEVSSPRFEEAQVDSSTSKDSIDVFVAAPRD


 


KVLQACTVTSGLMAALGLIIRKASHVASTEGLLVPDCSIDVPFGFETWHL


 


GLIAGIVVFISSSRFLLLKSWPDFADSSEAANRQILTSLEPLDYLVVAML


 


PGISEELLFRGALMPLFGTNWNGIVAVGLIFGLLHLGSGRKYSFAVWASI


 


VGIVYGYAAVLSSSLIVPMASHALNNLVGGLLWRYSSKIKSLE






cDNA sequence of AtFACE-2 from Arabidopsis (SEQ ID NO:5):








ATGGCCACCGATGGCGAGAGTATCTCGATGTCGTTGGCGGTGGCTACCTG


 


CGTCGCGATGGCACTATTCTACGTTTTGATCCTTTACGTTCCCACCGTGA


 


TACTCCGTGTCCCGTCGGCTTCTTCTTACACCGAATTCATGATTCGGCGA


 


TTCATCTGCGCGGCCATTTGTACTGTAGCATCTCTCGTCTTCACAGCTTT


 


TATACTTCCGATAAAAAGCTGGGAGGCCTCTTATATACTTGGAGTTTATG


 


GCATAAGGAAAGATCATCTGTGGCAAGGAGTGGTGTATCCTCTTCTATTG


 


ACCTCGCTCGTTTATGCTGGGTCTTTGGTGTTGAAGTTGTTTACTCTCCT


 


GGAATCATGGAAGGAAAATGGCGGAGGATGTAGTTCCTTTAATTACATCA


 


GAAGCTTTTTCCAAACAATCCCTGCTTCGGTATTGACAAGTGCTTCTAAT


 


GTTTCCGTTTGGCGCAATTTTATCGTGGCACCAGTAACTGAGGAGCTGGT


 


TTTCCGATCATGTATGATACCTTTGCTTCTGTGTGCTGGGTTTAGGATTA


 


ACACTGCCATCTTTCTGTGCCCAGTTCTCTTTAGCTTGGCTCACTTAAAC


 


CATTTTAGAGAGATGTACATCAGGCATAACCGCAGCTATCTCCGGGCTTC


 


ACTTATTGTTGGTCTTCAGCTTGGCTACACAGTCATTTTTGGTGCATATG


 


CATCGTTTCTCTTCATCAGAACCGGACATCTTGCTGCTCCTTTGTTTGCT


 


CATATATTTTGCAACTACATGGGATTGCCTGTGCTATACGCAAATGGAAA


 


AGGTTTGGTGAGTGCAGCGTTCTTAGGCGGTGTGGTTGGGTTCGTCTTAC


 


TTCTCTTTCCTTTAACAAAGCCTCTCATGTACAACGATAGTACCAACGAT


 


TGTCCGTGTTGGCTTGGCTATTGTTTGTGGAATTGA






The AtFACE-2 cDNA is translated into the following amino acid sequence (SEQ ID NO:6):








MATDGESISMSLAVATCVAMALFYVLILYVPTVILRLPSASSYTEFMIRR


 


FICAAICTVASLVFTAFILPIKSWEASYILGVYGIRKDHLWQGVVYPLLL


 


TSLVYAGSLVLKLFTLLESWKENGGGCSSFNYIRSFFQTIPASVLTSASN


 


VSVWRNFIVAPVTEELVFRSCMIPLLLCAGFRINTAIFLCPVLFSLAHLN


 


HEREMYIRHNRSYLRASLIVGLQLGYTVIFGAYASFLFIRTGHLAAPLFA


 


HIFCNYMGLPVLYANGKGLVSAAFLGGVVGFVLLLFPLTKPLMYNDSTND


 


GPGWLGYGLWN






cDNA sequence of ZM57353913 from corn (SEQ ID NO:7):








CGAAGCCACGCGACCGACTGTGTTACGATCCCAAATCTTCACTCCCGACG


 


AAATCTAGAATCCAATGAGCAATCTCGACTGACGCCTGCTTCACCAGATT


 


ATGGCGACGCCGGCGGGCCTCCTTCTCGCCTCGCCGCCGGTGATATCAGG


 


TGTCGCGGCGATGGCGGCGTGCGCCGCAATGGCAGTATTCTACGTCGCTG


 


TCCTCTATGC


 


CCCGACGGTCATCCTCCGGTTCCCACCCCCAACCTCACTCCGCACCTTCC


 


TCCACCGTCGCTTCGCCTGTGCCGCCGTCGCATCCGCCGCCTCCGTCCTT


 


GCCACTGCGTCCCTCCTCCGAGTCTGGAGCCTCAGCGACTTCGCTGATAT


 


GTTTGCTGTGTTCGGCATTAGGAAGGATCACTTGATTCAGGCCGTGGCTA


 


TTCCACTTCTCCTGACATCCCTAGTGTATGCTGGGTCATTCGTCGCTAGA


 


GTGTGGCTCCTAGTGAGCTCGTGGGGCGGTGGCGATGAGGTGGAGATAGG


 


CTGCGCACAGAGGCTTGCACAATGGATCCAAGCTGCTGTTGCGGATGTTA


 


TGGTTTGGCGGAACTATGTAGTGGCACCATTTACTGAGGAGCTGGTTTTC


 


AGGGCATGCATGATACCTCTTCTGCTCTGTGGGGGATTCAAAATGTCTAC


 


AATTATATTTCTGAGTCCAATCTTCTTCAGTCTAGCGCACTTGAACCATT


 


TTTTCGAACTACACCAGCAGGGATGTAACTTTATGAGAGCGCTATTGATT


 


GTAGGTGTCCAGTTAGGCTACACTGTCATTTTTGGGTGGTATGCAACATT


 


CTTGTTAATCCGAACAGGGAATCTGTTATGTCCAATTATTGCTCACGTCT


 


TCTGTAATATGATGGGTTTACCTGTTTTCTCGTCACCACGAACAAAAGGA


 


GCGGCATTGGTAGCGTTTCTGGCTGGTTCAATAGCCTTCTTTTGGCTGCT


 


TTTCCCTGCAACAAGTCCTGAACTGTACAACAGCAGTTTTGATCGCTGCA


 


GTTGCTGGCATGGCTTTTGCAATTGGAAATAACATAGAACTAGATTGGAA


 


AGCAATGGGTCCTAACTTGAAGCTACTAACAATTGAAACCTCCAGGCCCT


 


AGCTGACACTTCTGACGGATTTTCTATTTGCAGAAACTCCATATGAATGT


 


CTTAAAACGTTTTGTAGAAATGTGTCCCTTGTTTTAGCTTAAGACTCAAG


 


AGCTTAAACTAGCAAAAGATTGTATTTTGCCACTTGTTAAATACGTGCTG


 


ATCATGAAATCGCTGTCAATCCCTTCTCAAAGTGGAATTTGACTTTGTTG


 


AGCTGCTTTTATTTATATTGTGCTTGCTACTGCAGCGCCTAGAGTTTGTA


 


GATTACACATCATGGACCCGTCTGATATTGTAAACGAGAGACATGTTTCT


 


AAGTTAATATGCTCCCTCCATTTATTTAAAAAAAAAAAAAA






The ZM57353913 cDNA is translated into the following amino acid sequence (SEQ ID NO:8):








MATPAGLLLASPPVISGVAAMAACAAMAVFYVAVLYAPTVILRFPPPTSL


 


RTFLHRRFACAAVASAASVLATASLLRVWSLSDFADMFAVFGIRKDHLIQ


 


AVAIPLLLTSLVYAGSFVARVWLLVSSWGGGDEVEIGCAQRLAQWIQAAV


 


ADVMVWRNYVVAPFTEELVFRACMIPLLLCGGFKMSTIIFLSPIFFSLAH


 


LNHFFELHQQGCNFMRALLIVGVQLGYTVIFGWYATELLIRTGNLLCPII


 


AHVFCNMMGLPVFSSPRTKGAALVAFLAGSIAFFWLLFPATSPELYNSSF


 


DRCSCWHGFCNWK






cDNA sequence of ZM59252659 from corn (SEQ ID NO:9):








CCCAAATCTTCATTTCCGACGAAATCGAGAATCCAATGTGCAATCTCGAC


 


TGACGCCTGCTTCAACAGATTATGGCGACGCGGTGGGTCTCCTTCTCGCC


 


TCGCCGCCGGAATATCAGGGTCGCGCGATGGGTCGTGCGCCAACGGAAGG


 


ATTCTACGTCGCTGTCCTCTATGCCCCGACGGTCATCCTCCGGGTCCCAC


 


CCCCAAGCTCACTCCGCACCTTCCTCCACCGTCGCTTCGCCTGTGCCGCC


 


GTCGCATCCGCCGCCTCCGTCCTTGCCACTGCGTCCCTCCTCCGAATCTG


 


GAGCCTCAGCGACTTCGCTGATATGTTTGCTGTGTTCGGCATTAGGAAGG


 


ATCACTTGATTCAGGCCGTGGCTATTCCACTTCTCCTGACATCCCTAGTG


 


TATGCTGGGTCATTCGTCGCTAGAGTGTGGCTCCTAGTGAGCTCGTGGGG


 


CGGTGGCGATGAGGTGGAGATAGGCTGCGCACAGAGGCTTGCACAATGGA


 


TCCAAGCTGCTGTTGCGGATGTTATGGTTTGGCGGAACTATGTAGTGGCA


 


CCATTTACTGAGGAGCTGGTTTTCAGGGCATGCATGATACCTCTTCTGCT


 


CTGTGGGGGATTCAAAATGTCTACAATTATATTTCTGAGTCCAATCTTCT


 


TCAGTCTAGGTGTCCAGTTAGGCTACACTGTCATTTTTGGGTGGTATGCA


 


ACATTCTTGTTAATCCGAACAGGGAATCTGTTATGTCCAATTACTGCTCA


 


CGTCTTCTGTAATATGATGGGTTTACCTGTTTTCTCGTCACCACGAACAA


 


AAGGAGCGGCATTGGTAGCGTTTCTGGCTGGTTCAATAGCCTTCTTTTGG


 


CTGCTTTTCCCTGCAACAAGTCCTGAACTGTACAACAGCAGTTTTGATCG


 


CTGCAGTTGCTGGCATGGCTTTTGCAATTGGAAATAACATAGAACTAGAT


 


TGGAAAGCAATGGGTCCTAACTTGAAGCTACTAACAATTGAAACCTCCAG


 


GCCCTAGCTGACACTGCTGACGGATTTTCTATTTGCAGAAACTCCATATG


 


AATGTCTTAAAACGTTTTGTAGAAATGTGTCCCTTGTTTTAGCTTAAGAC


 


TCGAGCTTAAACTAGCAAAAGATTGTATTTTGCCACTTGTTAAATACGTG


 


CTGATCATGAAATCGCTGTCAATCCCTTCTCAAAGTGGAATTTGACTTTG


 


TTGTAAAAAAAAAAA






The ZM59252659 cDNA is translated into the following amino acid sequence (SEQ ID NO:10):








MGRAPTEGFYVAVLYAPTVILRVPPPSSLRTFLHRRFACAAVASAASVLA


 


TASLLRIWSLSDFADMFAVFGIRKDHLIQAVAIPLLLTSLVYAGSFVARV


 


WLLVSSWGGGDEVEIGCAQRLAQWIQAAVADVMVWRNYVVAPFTEELVFR


 


ACMIPLLLCGGFKMSTIIFLSPIFFSLGVQLGYTVIFGWYATFLLIRTGN


 


LLCPITAHVFCNMMGLPVFSSPRTKGAALVAFLAGSIAFFWLLFPATSPE


 


LYNSSFDRCSCWHGFCNWK






cDNA sequence of CASAR82A from pepper (SEQ ID NO:11):








ATGGTGTCTAAGTCCTCAATCTTCATTTGCCTGAGCCTTATCATCCTCGT


 


GATCATGTCTACCCAGATCGTGGCTAGAGAGATGACCAGTGAAGCTTCTG


 


CTTCACTCACACAGGCAATGAACGGGAACAATATCTCTGAGACCAAGAAA


 


GTGGGTCGTCACTTGGTGAAGGGCTTGGATAAGATCTTCAAGGCTGGAAA


 


GGTGATCTACTGCAAGACCTGCAAAACCTGCCACGGTCGCTGCGATTACT


 


GTTGCGCC






The CASAR82A cDNA is translated into the following amino acid sequence (SEQ ID NO:12):








MVSKSSIFICLSLIILVIMSTQIVAREMTSEASASLTQAMNGNNISETKK


 


VGRHLVKGLDKIFKAGKVIYCKTCKTCHGRCDYCCA






cDNA sequence of b3358 from E. coli (SEQ ID NO:13):








ATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACA


 


AACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAAT


 


TACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCG


 


GGCCTTGATA


 


CGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTT


 


GCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCT


 


GCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCGTCACTGCTGAGC


 


TGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATT


 


TTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCAT


 


CTATGCGTTGGGCACTCTCTGGTACGGATTGTTTAACTGGTTTTGGTTCT


 


GGATCTGGCGCGAACAACCGCTGCGCGAGTCACTAAGTCTGCTGTACCGT


 


GAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCACAC


 


CGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGG


 


TCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAA


 


AATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGA


 


TTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAA


 


AGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAA


 


ACCGTCGCCGCTCGCCTGCGCGTGCTGGCTGATGACATTCTTTACCATCG


 


CCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAA


 


TCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCAT


 


TTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCG


 


TGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGA


 


AAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTC


 


AGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCACCGCGCTGCATCTGCC


 


GAAGTCGTACTGGATCCTGATGACGGTATTGCTGGTGACACAAAATGGCT


 


ATGGCGCAACCCGTCTGAGGATTGTGAATCGCTCCGTGGGAACCGTGGTC


 


GGGTTAATCATTGCGGGCGTGGCGCTGCACTTTAAAATTCCCGAAGGTTA


 


CACCCTGACGTTGATGCTGATTACCACCCTCGCCAGCTACCTGATATTGC


 


GCAAAAACTACGGCTGGGCGACGGTCGGTTTTACTATTACCGCAGTGTAT


 


ACCCTGCAACTATTGTGGTTGAACGGCGAGCAATACATCCTTCCGCGTCT


 


TATCGATACCATTATTGGTTGTTTAATTGCTTTCGGCGGTACTGTCTGGC


 


TGTGGCCGCAGTGGCAGAGCGGGTTATTGCGTAAAAACGCCCATGATGCT


 


TTAGAAGCCTATCAGGAAGCGATTCGCTTGATTCTTAGCGAGGATCCGCA


 


ACCTACGCCACTGGCCTGGCAGCGAATGCGGGTAAATCAGGCACATAACA


 


CTCTGTATAACTCATTGAATCAGGCGATGCAGGAACCGGCGTTTAACAGC


 


CATTATCTGGCAGATATGAAACTGTGGGTAACGCACAGCCAGTTTATTGT


 


TGAGCATATTAATGCCATGACCACGCTGGCGCGGGAACACCGGGCATTGC


 


CACCTGAACTGGCACAAGAGTATTTACAGTCTTGTGAAATCGCCATTCAG


 


CGTTGTCAGCAGCGACTGGAGTATGACGAACCGGGTAGTTCTGGCGATGC


 


CAATATCATGGATGCGCCGGAGATGCAGCCGCACGAAGGCGCGGCAGGTA


 


CGCTGGAGCAGCATTTACAGCGGGTTATTGGTCATCTGAACACCATGCAC


 


ACCATTTCGTCGATGGCATGGCGTCAGCGACCGCATCACGGGATTTGGCT


 


GAGTCGCAAGTTGCGGGATTCGAAGGCGTAA






The b3358 cDNA is translated into the following amino acid sequence (SEQ ID NO:14):








MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVPACCNIA


 


GLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGV


 


TAELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNW


 


FWFWIWREQPLRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQ


 


QKAVDLITQCYQQMHMLSAQNNTDYKRMLRIFQEALDLQEHISVSLHQPE


 


EVQKLVERSHAEEVIRWNAQTVAARLRVLADDILYHRLPTRFTMEKQIGA


 


LEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPLYARDLLADKQRRMPLL


 


PALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWILMTVLLVT


 


QNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY


 


LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGG


 


TVWLWPQWQSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQ


 


AHNTLYNSLNQAMQEPAFNSHYLADMKLWVTHSQFIVEHINAMTTLAREH


 


RALPPELAQEYLQSCEIAIQRCQQRLEYDEPGSSGDANIMDAPEMQPHEG


 


AAGTLEQHLQRVIGHLNTMHTISSMAWRQRPHHGIWLSRKLRDSKA






cDNA sequence of EST564 from moss (SEQ ID NO:15):








ATGTCATGCGACGTTCTCTGCCAATCTTTCAAGGAGGTAGAGGGCAAGTT


 


CTTGGAAATCGTCGAAAGGGCTTGGGCCGTCAAGCCGCAAATTGCCGCTG


 


TTGGATCTTGTTGTTTGGTGGGAGCCGTATGGGATTCCAAACTGTACATC


 


GCTAGTCTTGGAGATTCTCGAGCTGTTTTAGGTAGTTGCTCTCGTGACAC


 


TGGCCTTCCAGTTGCTAAGCAAATTTCAACAGAGCACAACGCAAGCATCG


 


AGTCTATCCGGAATGAGTTGTTCGCAAAGCATAGTGATGATCCGCAGATC


 


GTGGTTTTGAAGCATGGAGTGTGGCGTGTGAAGGGTATTATTCAGATTTC


 


ACGCTCAATTGGTGATTTTTACTTGAAGAAAGCCGAATTTAATCAGCCGC


 


CTCTTATAGCCAGGTTCCGG


 


CTTCCAGATCCCCTCAAGAGACCTGTCATAAGCTCAGAGCCGGAGTGCAA


 


CGTCATTACACTCGGCCCGGATGACGAATTCGTCATTTTTGCATCTGATG


 


GCCTTTGGGAGCACTTGAGCAGCAAAGAGGCCGTAGACATTGTGTATAGT


 


CATCCCCGGGCTGGGATTGCCAGGCGTCTGATCAAAGCTGCTCTTCAAAA


 


AGCTGCTACTAAACGTGAAATGCGGTACTCTGATTTGAAAGGGATTGAGC


 


GCGGGATACGACGGCATTTTCATGATGACATAACTGTTGTGGTTCTTTAT


 


TTGGACACTAAACTGCTCAACAGAGGTGGTAGTATTTCTAATCATATTTC


 


TTCGAAATGTCCAATTGACATGCCAAAAGGCGATAACCCTCCGTCGTTAG


 


TTAGCTCTAACATGAACTTAGCTTTTAACAAATAA






The EST564 cDNA is translated into the following amino acid sequence (SEQ ID NO:16):








MSCDVLCQSFKEVEGKFLEIVERAWAVKPQIAAVGSCCLVGAVWDSKLYI


 


ASLGDSRAVLGSCSRDTGLPVAKQISTEHNASIESIRNELFAKHSDDPQI


 


VVLKHGVWRVKGIIQISRSIGDFYLKKAEFNQPPLIARFRLPDPLKRPVI


 


SSEPECNVITLGPDDEFVIFASDGLWEHLSSKEAVDIVYSHPRAGIARRL


 


IKAALQKAATKREMRYSDLKGIERGIRRHFHDDITVVVLYLDTKLLNRGG


 


SISNHISSKCPIDMPKGDNPPSLVSSNMNLAFNK






cDNA sequence of BN49502266 from canola (SEQ ID NO:17):








CCAATAATCAAATCAAAACCCTTTCGATCAGTTGTTNCAGGAAAAAAAAA


 


AACCCTTTCGATCTCATTCCATTTCGAATCAGAAAACCCTAGCAATTGAC


 


GATGTTGCGAGCTTTAGCGCGGCCTCTCGAACGGTGTTTAGGAAGCAGAG


 


CGAGCGGCGACGGTTTGCTCTGGCAATCGGAGTTGAAACCACACGCCGGC


 


GGAGATTACTCGATCGCGGTGGTTCAAGCCAATTCTAGCCTAGAGGATCA


 


GAGTCAGGTGTTCACGTCTTCCTCCGCTACTTACGTCGGCGTCTACGACG


 


GCCATGGCGGACCCGAAGCTTCTAGATTCGTTAACAGACATCTCTTTCCT


 


TATATTCAGAAGTTCGCAAAAGAACATGGAGGACTGTCTGCAGACGTTAT


 


TAAAAAAGCATTCAAAGAAACTGAAGAGGATTTTTGCGGTATGGTTAAAC


 


GCTCACTTCCCATGAAGCCACAGATGGCTACTGTAGGATCTTGCTGTCTC


 


TTTGGTGCCATCTCTAACGGCACGCTCTATGTCGCGAATCTTGGAGACTC


 


GAGAGCCGTTCTTGGGAGCGTTGTTGCAGGGGATGATAGTAATAGTAGTA


 


ACAAGGGTGCTGCAGCTGAACGGTTGTCCACTGATCATAACGTTGCTGTT


 


GAAGAAGTGAGGAAGGAGGTTAAGGAACTTAACCCGGATGATTCGCAGAT


 


CGTCATGTACATTCGTGGAGTTTGGAGGATTAAAGGCATTATTCAGGTAT


 


CTAGATCAATTGGGGATGTTTACTTGAAGAAACCGGAGTTTTACAGGGAT


 


CCGATATTCCAGCAACATGGAAATCACATTCCTTTGAGGAGACCCGCGAT


 


GACAGCTGAACCGTCCATTATAGTAAGGAAGCTTAAGCCGCAAGACTTGT


 


TTCTGATATTTGCATCAGATGGTCTCTGGGAGCATCTTAGTGATGAAGCA


 


GCAGTAGAAATTGTACTCAAACACCCAAGAACTGGGATTGCAAGAAAACT


 


TGTAAGAGCAGCTCTTGAAGAAGCAGCAAGGAAGAGAGAAATGAGATATG


 


GAGATATAAAGAAAATAGCCAAAGGGGTTAGAAGACATTTCCATGACGAC


 


ATAAGCGTCGTTGTAGTTTATCTTGATCAACAAAAAACCACTTCTTCATC


 


GAATGATAGATTGATCCAGAAAGGAGGAATCACTGCTCCACCGGATATCT


 


ACTCGTTACGTTCAGATGAAGCTGAGCAACGACGGCTACTCAATGTGTTA


 


TATTGATACTCTCTGGTTAGAGGGATACAACTTGTTTACATATTTGTTTA


 


ATCTTTTACAAAGAATGTTTGTTCTTTTTTCTTTCTTTTTTTAATATTTG


 


GAGTTGGATTTGTATATTCTTTTTACCAGCAAGGAACGAAAACCCTTCTC


 


TTTTGGGGGCAAAACAGTTTTGGTTTTGACAAACAATATAAAGTGAAACC


 


GTTTGCAAAAAAAAAAAAAAAAA






The BN49502266 cDNA is translated into the following amino acid sequence (SEQ ID NO:18):








MLRALARPLERCLGSRASGDGLLWQSELKPHAGGDYSIAVVQANSSLEDQ


 


SQVFTSSSATYVGVYDGHGGPEASRFVNRHLFPYIQKFAKEHGGLSADVI


 


KKAFKETEEDFCGMVKRSLPMKPQMATVGSCCLFGAISNGTLYVANLGDS


 


RAVLGSVVAGDDSNSSNKGAAAERLSTDHNVAVEEVRKEVKELNPDDSQI


 


VMYIRGVWRIKGIIQVSRSIGDVYLKKPEFYRDPIFQQHGNHIPLRRPAM


 


TAEPSIIVRKLKPQDLFLIFASDGLWEHLSDEAAVEIVLKHPRTGIARKL


 


VRAALEEAARKREMRYGDIKKIAKGVRRHFHDDISVVVVYLDQQKTTSSS


 


NDRLIQKGGITAPPDIYSLRSDEAEQRRLLNVLY






cDNA sequence of GM49788080 from soybean (SEQ ID NO:19):








TCCCGGGTCGACGATTTCGTGGTTACGGGGCGGAAGGAAGGGCTGCTGTG


 


GTACAAGGATGCGGGGCAGCACTTGTTTGGTGAATACTCAATGGCTGTTG


 


TCCAGGCCAACAACCTGCTCGAGGACCAGAGCCAGATTGAGTCTGGTCCT


 


CTCAGCCTGCTTGACACTGGCCCTTATGGGACCTTTGTTGGTGTATATGA


 


TGGACACGGTGGGCCCGAGACGTCGCGCTACGTCTGTGATCATCTCTTCC


 


AACATCTAAAACGATTTGCATCTGAGCAGAAGTCCATGTCTATGGAGGTT


 


ATTCGGAAGGCATACCAAGCCACAGAAGAAGGTTTTTTGTCAGTGGTTAC


 


CAAACAGTGGCCCATGAATCCCCAAATTGCTGCTGTGGGATCTTGTTGTT


 


TGGTTGGTGTGATTTGTGGTGGTATCCTCTATATTGCTAACCTTGGTGAT


 


TCCCGTGCTGTGCTTGGCCGGGTGGTCAGAGCAACTGGGGAGGTTTTGGC


 


GATCCAGCTTTCGTCAGAGCATAATGTGGCCATAGAATCTGTGAGACAAG


 


AGATGCATTCTTTGCATCCGGATGACTCAAAAATTGTGGTTCTAAAGCAC


 


AATGTATGGCGGGTGAAGGGTCTGATACAGATTTCTAGATCCATTGGCGA


 


TGTATACCTAAAAAAGGCTGAATTTAACAAGGAACCGTTGTATGCTAAGT


 


TTCGTGTGCGGGAAGGTTTTAAGAGGCCCATTTTGAGCTCTGACCCATCA


 


ATTTCTGTCCATGAACTTCAACAGCATGATCAATTTCTCATATTTGCTTC


 


TGATGGTCTTTGGGAACACCTTAGCAATCAGGATGCCGTTGATATAGTTC


 


AAAACAACCCACACAATGGAATTGCTCGGAGGCTCATCAAAGCTGCGTTG


 


CAAGAAGCAGCAAAAAAGAGAGAGATGAGGTACTCTGATTTGAAGAAAAT


 


TGACCGTGGTGTCCGCCGGCATTTCCATGATGACATCACAGTTGTAGTTG


 


TATTTCTTGACTCCAATCTTGTCAGCAGAGCCAGCTCAGTAAGAGGTCCT


 


CCTTTATCGGTGAGAGGAGGTGGTGTTCCCCTACCTTCTAGAACTTTGGC


 


TCCCTGTGCTGCACCTATGGAAACTTAGTTCAGGTTGATGAAGCTGGCTG


 


TATGATCTGTTATGCTTCTATTTAGTGTTGTACCCTTAGCAGACATTGAG


 


CTCTGGTGATCCACCAGATTGTATATCCAATTTAACAGAGATTGAAAAAA


 


TGTTCGTTCA


 


ATTAGTACAATGTTACAAGTGACTTGGGGTATGTAGCTTGCGTGAGTAAA


 


GCATCATG-GAA






The GM49788080 cDNA is translated into the following amino acid sequence (SEQ ID NO:20):








MAVVQANNLLEDQSQIESGPLSLLDTGPYGTFVGVYDGHGGPETSRYVCD


 


HLFQHLKRFASEQKSMSMEVIRKAYQATEEGFLSVVTKQWPMNPQIAAVG


 


SCCLVGVICGGILYIANLGDSRAVLGRVVRATGEVLAIQLSSEHNVAIES


 


VRQEMHSLHPDDSKIVVLKHNVWRVKGLIQISRSIGDVYLKKAEFNKEPL


 


YAKFRVREGFKRPILSSDPSISVHELQQHDQFLIFASDGLWEHLSNQDAV


 


DIVQNNPHNGIARRLIKAALQEAAKKREMRYSDLKKIDRGVRRHFHDDIT


 


VVVVFLDSNLVSRASSVRGPPLSVRGGGVPLPSRTLAPCAAPMET






cDNA sequence of GM53049821 from soybean (SEQ ID NO:21):








TGCTCCTCTACCACCGAACACANCCCCGGCCACCACCGAACGCTAACGTG


 


CGCCCCTTCCTTACCCTGCGCCTCGGCACTCTCCCTTATTCCCCCTCCTT


 


CATAAGCTCCGCGTTAACCGTCCTCTCTCTCTCTCTCTCTCGGATCGGAG


 


CGAGACTGGCGGCTCCGGCGTTGGGGGCGTTAGGGTTAGGGTTAGGGTTT


 


CCAAGAGATG


 


TGGTATGCTCCAGGCATTGATGAATCTGTTCTCGCTGTGTTGGAAGCCAT


 


TTGGCCGCGATGCTGCTGATCGAATCGATTCCATCGGAGTTACCGGAAGA


 


GAAGGCAAAGACGGCTTGCTTTGGTTCCGCGACGGCGGAAAATATGGCTC


 


TGGCGATTTCTCCATGGCCGTCGTTCAGGCCAACCAGGTTCTCGAAGACC


 


AGAGCCAGATCGAGTCTGGTCCTCTCGGCACCTTCGTCGGCATCTACGAC


 


GGTCACGGAGGACCCGACGCCTCAAGATACGTTTGCGATCACTTGTTTCG


 


CCATTTTCAAGCAATATCAGCTGAGTCACGCGGGGTTGTGACAACTGAGA


 


CAATCGAAAGAGCATTTCGCCAAACAGAAGAGGGGTACATGGCCCTCGTG


 


TCAGGCTCGTGGAATGCTCGACCTCATATTGCAAGTGCTGGGACCTGTTG


 


TCTAGTTGGAGTGATATTTCAGCAGACACTCTTTGTGGCAAACGCTGGAG


 


ATTCCCGTGTTGTATTGGGTAAGAAAGTTGGCAACACTGGAGGTATGGCT


 


GCAATTCAGCTGTCTACAGAACACAATGCAAATCTTGAGGCTGTTAGGCA


 


GGAACTTAAAGAATTACATCCTCATGATCCCCAAATTGTTGTCCTCAAAC


 


ATGGAGTGTGGAGAGTAAAAGGCATTATTCAGGTTTCTAGATCTATAGGT


 


GATGTATATTTGAAGCATGCACAGTTTAACCGAGAACCACTTAATGCAAA


 


ATTCAGACTTCCTGAACCGATGAACATGCCTATCTTGAGTGCTAATCCCA


 


CTATTCTTTCTCATGCTCTCCAACCAAATGATTCCTTCCTTATATTTGCA


 


TCTGATGGTTTATGGGAGCATTTGAGTAACGAGAAAGCTGTGGATATTGT


 


AAACAGCAATCCACATGCGGGTAGTGCCAAGAGACTTATCAAGGCTGCTC


 


TCCATGAAGCAGCAAGAAAACGAGAAATGCGATATTCAGACCTCCGTAAG


 


ATTGACAAGAAAGTTCGACGCCATTTTCATGATGATATATCCGTTATTGT


 


TTTATTCTTAAATCACGACCTTATTTCCAGAGGCACAGTGCTAGACCCGA


 


CACTTTCAATTCGAAGCGCTCTCGATCACTGACTTGTATCACTGTAAGCA


 


GTCTTGTACGAGTTTTTGGCAACTGTACCGATACCTGAAGCATTGGTAGG


 


TACCTGGCTATAATATGTCATTTCTATGGCACATATGGCTTCTGGTACCG


 


ACATCATTCT


 


TGAGGCACGAGAATTTATTAAGTTATAACATATTATTAGAAATTTATTCA


 


TAAAGAGGAAAAAAATAAATACAAAAATATCTTATTCCCTTTTCTAACCT


 


TATAGTTTTACCCGAAATACTGGATTTTATTTATTTGTTTGTTTTTTTGG


 


CTGAACATAGCTAATCGAACAGCATGTTGATTGAATTCAAAGTTATTTTA


 


CAACAAATTATATGGAAAAAAAAAAAAAAA






The GM53049821 cDNA is translated into the following amino acid sequence (SEQ ID NO:22):








MLQALMNLFSLCWKPFGRDAADRIDSIGVTGREGKDGLLWFRDGGKYGSG


 


DFSMAVVQANQVLEDQSQIESGPLGTFVGIYDGHGGPDASRYVCDHLFRH


 


FQAISAESRGVVTTETIERAFRQTEEGYMALVSGSWNARPHIASAGTCCL


 


VGVIFQQTLFVANAGDSRVVLGKKVGNTGGMAAIQLSTEHNANLEAVRQE


 


LKELHPHDPQIVVLKHGVWRVKGIIQVSRSIGDVYLKHAQFNREPLNAKF


 


RLPEPMNMPILSANPTILSHALQPNDSFLIFASDGLWEHLSNEKAVDIVN


 


SNPHAGSAKRLIKAALHEAARKREMRYSDLRKIDKKVRRHFHDDISVIVL


 


FLNHDLISRGTVLDPTLSIRSALDH






cDNA sequence of ZM58462719 from corn (SEQ ID NO:23):








CGTGGCGACGCCCAAATCGAGCGACCTGATCGAGGCCCCTCGCCCCTACT


 


CGCTGAATCCCAATCCGAGCCCGCCAATTGGGCGCCCCCCCCCGCCCACG


 


CAAAGGACAGATAGAAGAAAATTATTGGCGCTCTGACAAATCCAACTGAG


 


GTTTTCTTGGACTACAGATGAAGCGGGCTCGAAGGGCGTATGTGCAAGAG


 


ATGACTGATGAGGGATGCTAGTGAAATTGATGAACTTGTTACGGGCGTGC


 


TGGCGACCGTCATCGAACCGGCATGCCCGAACAGGCTCAGATGTTACCGG


 


TAGGCAGGATGGACTTCTATGGTACAAGGACGCCGGGCAACATGTCAATG


 


GGGAGTTCTCCATGGCTGTTGTTCAGGCAAATAACTTACTTGAGGACCAG


 


TGTCAGATCGAGTCGGGCCCACTGAGTTTTCTAGATTCTGGACCATATGG


 


CACTTTCGTTGGTGTTTACGATGGGCATGGTGGTCCAGAGACGGCCTGCT


 


ATATCAATGATCATCTTTTCCAGAATCTGAAAAGATTTGCATCTGAACAG


 


AATGCAATGTCTGCTGATGTACTGAAGAAGGCATATGAAGCTACAGAAGA


 


TGGATTCTTCTCCATTGTTACCAAACAATGGCCTGTAAAGCCTCAGATAG


 


CAGCTGTCGGCTCATGCTGCCTGGTCGGTGTAATTTGTGGTGGCATGCTT


 


TATGTTGCCAATGTTGGGGATTCCCGTGTCGTTTTAGGAAAACATGTTAA


 


GGCCACTGGAGAAGTTTTGGCTGTCCAACTGTCAGCAGAACATAATGTTA


 


GTATTGCGTCCGTGAGAAAAGAACTGCAGTCAATGCACCCAGAAGATAGG


 


CACATTGTTGTTCTCAAGCACAATGTTTGGCGTGTTAAAGGACTAATTCA


 


GGTTTGTAGATCAATTGGTGATGCATATCTCAAAAAGCAAGAGTTCAACA


 


GGGAACCCCTATATGCAAAATTTCGCCTCCGTGAACCTTTTCACAAGCCA


 


ATACTAAGTTCAGAACCATCAATCAGTGTGCAACCACTACAACCACACGA


 


CCAGTTTCTCATATTTGCATCTGATGGACTTTGGGAGCAGTTAACCAACC


 


AAGAGGCAGTTGATATTGTTCGAAGTAGCCCCCGCAGTGGCTGTGCTAGG


 


AGGCTGATAAGAGCGGCACTGCAAGAGGCAGCCAAGAAAAGAGAGATGAG


 


GTACTCGGACCTCAAGAAGATTGACCGCGGTGTTCGCCGCCACTTCCACG


 


ACGACATAACAGTCATAGTAGTGTTCCTTGACTCCGGCCTCGTAAGCCAG


 


GCGAGCACACACCGAGGTCCAACTCTTTCCTTGCGAGGCGGTGGCGGCAG


 


CGCTGGCCTGCGCAGCAACACACTTGCACCTACGTGACTATAAAGTGCCT


 


GGTGGAGTGGAGGCTACTGACTGAAGGTGGTTTTCTTTCCTTGTGTCGAA


 


TGTGTTATATATGTACTTGTACCAGCCAAGATCATTCATCCCCCCCCCTA


 


AAATGGTGTAAAGAAGTAGGAGAGGCGCCGAAGTTCCTCACCAGCGTATC


 


TGAATGCCCTCAATGGTGTCAAGTTGTGGACTCAAGTGGATAGCTTCGCT


 


GAATCTTCTGATGATGCTCTGTGGAAAGCTCGAATCCTTTCCACCTGAAA


 


AAGCAAGTAATATGTCTTCCAGTGCTGGAATTAACCCCTAGTGCATATAT


 


ATATGTATGAAATAATAATAAGGCAAAAGGAGGAGTAACTTATTTAACTA


 


ATGCTGTGAGGTGTATTTATGTTTTGTATGTGTACTGCTTTTGACTGCTA


 


CTGCATCTACTGTTGTTAATTGACCACTGGTGAAGTGAAATCACTGGTTT


 


CGTAAAAAAAAAAAAAAA






The ZM58462719 cDNA is translated into the following amino acid sequence (SEQ ID NO:24):








MLVKLMNLLRACWRPSSNRHARTGSDVTGRQDGLLWYKDAGQHVNGEFSM


 


AVVQANNLLEDQCQIESGPLSFLDSGPYGTFVGVYDGHGGPETACYINDH


 


LFQNLKRFASEQNAMSADVLKKAYEATEDGFFSIVTKQWPVKPQIAAVGS


 


CCLVGVICGGMLYVANVGDSRVVLGKHVKATGEVLAVQLSAEHNVSIASV


 


RKELQSMHPEDRHIVVLKHNVWRVKGLIQVCRSIGDAYLKKQEFNREPLY


 


AKFRLREPFHKPILSSEPSISVQPLQPHDQFLIFASDGLWEQLTNQEAVD


 


IVRSSPRSGCARRLIRAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITV


 


IVVFLDSGLVSQASTHRGPTLSLRGGGGSAGLRSNTLAPT






cDNA sequence of ZM61092633 from corn (SEQ ID NO:25):








AGCTTCCTCCCTCTTCCCTGGTCTGGTCGCTTCTCCTGTAGCTGTAATTT


 


TTGAGAGTCCCTCTCAAACTTTGCTTGCTTGCGCTCTCCATATATCCTGT


 


GGATCGGAGAGGATGCTCTGATCTACCTGTCTGTTCTTCGATCGAGTCTG


 


AGAGATTTGGGAGGAGGAGGGAAACAAAGCGAAAGAGCCCATCTTTTTTG


 


TCTTTTTGGTTCGGTTTCGTGGTTGCTTCTTTTGGACCCCGCGGAGGAGC


 


CCACCGTTTCTACAAAAACCCAATCTTTGCTGCCTTCTCAGCGGTCGAGA


 


TCGATAGGTTTCCAGATCTGAGGCTCCGTGTTCTGGCTGTGAGATCGGAG


 


GCGCAGCAATCCGAGCACGCAGCTAGTAGGGAAAGTATCCGAGAAAAGTT


 


GCAGATTTTGCTGGGGGCAACGGAGCGAGAACAAGTTACTGCAGAAGGAA


 


AGGGCAAAGGTGGGGGAGGCGCCGGAGATGAGGGATGCTATCAGCTCTGA


 


TGGATTATTTGAAATCTTGCTGGGGTCCGGCATCACCGGCTGGGCGTCCC


 


CGCAAAGGATCGGATGCCACCGGCCGCCAGGACGGGCTCCTGTGGTACAA


 


GGACGGCGGGCAGGTCGTCGATGGTGAGTTCTCCATGGCCGTGGTCCAGG


 


CCAATAACCTATTGGAGGACCATAGCCAGGTTGAATCCGGGCCGCTTAGC


 


ACATCGGAGCCTGGACTGCAAGGCACCTTCGTCGGGGTCTACGATGGGCA


 


CGGTGGCCCGGAGACAGCGCGTTACATCAATGACCATCTCTTCAACCACT


 


TGAGGAGATTCGCATCTGAGCACAAGTGCATGTCAGCGGATGTGATTCGG


 


AAGGCATTCCGAGCGACTGAGGAGGGTTTCATTTCTGTGGTTAGTAACCA


 


ATGGTCATTGAGACCTCAATTAGCAGCTGTAGGCTCTTGCTGTCTAGTTG


 


GTGTGGTTTGCAGCGGAACTCTATATGTTGCAAACCTTGGGGACTCCCGT


 


GCTGTTCTGGGGAGACTTGTCAAGGGAACTGGGGAGGTTTTGGCAATGCA


 


GCTCTCAGCAGAACACAATGCATCCTATGAGGAGGTTAGACGAGAGCTGC


 


AGGCATCACATCCTGATGATCCCCATATTGTGGTCCTAAAACACAATGTT


 


TGGCGTGTAAAGGGTATTATCCAGATAACAAGGTCAATTGGAGATGTGTA


 


TCTGAAGAAACCAGAATTTAATAGAGAACCTTTGCACAGCAAGTTTCGTC


 


TTCAGGAAACTTTTAGGAGACCACTTCTTAGTTCTGATCCAGCAATTACT


 


GTCCACCAAATACAGCCAACTGATAAGTTCATCATTTTTGCATCTGATGG


 


ACTCTGGGAACATCTTAGTAATCAGGAAGTGGTTGACATGGTCCAAAGTA


 


GCCCGCGTAATGGAATCGCACGAAAGTTAGTAAAGTCTGCAGTGCAGGAA


 


GCAGCGAAGAAGAGGGAGATGCGGTATTCAGACCTCAAGAAAGTTGATCG


 


GGGGGTGAGGCGGCACTTCCACGACGATATAACTGTCATTGTGGTATTTT


 


TCGATTCAAACGCCATGACAACTGCTGCCTGGAGCAGACCCTCGGTCTCT


 


CTCCGAGGGGGTGGGTTTCCAATCCATTCAAACACCCTTGCTCCATTCTC


 


GGTTCCTACAGAGCTAAACAACTCCTACTGAAACCACGCGGTATGTGAAG


 


GAGCCAGGCAAGAGGATAAAAAAAAAGTAAAGGAAAACGGAGAAGGAAAA


 


ACAGCTGTTGTGATCAGTTGTAGTGTATTTCACCGTTCATGTTCATTTAA


 


AACATTTTTTAGATTCTCAAGTCTCAACCTGGTGACCAGTGCACTGATAG


 


CAAGGTATAAGATTAGATTATTCTTAGCTTTTTTATCCTCTTTTTTTTTT


 


CTCGTCCTTACCCTTTAGATTCACTCATGGGATATCCGATATCAGGTGCT


 


TGTACATTCTTTGGTTCAACTTGTGATAATAGTTCATCGCCCCCCTCTTT


 


TCGCAAAAAAAAAAA






The ZM61092633 cDNA is translated into the following amino acid sequence (SEQ ID NO:26):








MLSALMDYLKSCWGPASPAGRPRKGSDATGRQDGLLWYKDGGQVVDGEFS


 


MAVVQANNLLEDHSQVESGPLSTSEPGLQGTFVGVYDGHGGPETARYIND


 


HLFNHLRRFASEHKCMSADVIRKAFRATEEGFISVVSNQWSLRPQLAAVG


 


SCCLVGVVCSGTLYVANLGDSRAVLGRLVKGTGEVLAMQLSAEHNASYEE


 


VRRELQASHPDDPHIVVLKHNVWRVKGIIQITRSIGDVYLKKPEFNREPL


 


HSKFRLQETFRRPLLSSDPAITVHQIQPTDKFIIFASDGLWEHLSNQEVV


 


DMVQSSPRNGIARKLVKSAVQEAAKKREMRYSDLKKVDRGVRRHFHDDIT


 


VIVVFFDSNAMTTAAWSRPSVSLRGGGFPIHSNTLAPFSVPTELNNSY






cDNA sequence of ZM62016485 from corn (SEQ ID NO:27):








TGTCTTGCTGCTGGCGCGCCGGGGGCTCCGATTGCGCTCCAGATCTGAGG


 


CACCTGCTCGGTGGATTCCAGGAATCCGAGCACCAACTCGACAGGGGAGT


 


TCTCAGGGTAAAGAGGCTGAGAGCGCGTTGGAGATTTGGACTATAAGAGC


 


GAGCGAGCGAGCTGGGTGCCTTGCTGCCTTGAGGACGCCGTCAAGAAACC


 


GCGTGGAGGGGAGGGCGATGAGATGAGGGATGCTGGCCGCGGTGATGGAC


 


TACTTCAGCACCTGCTGGGGCCCGCGGTCTCGTGCGGGGCACCGGGGCAA


 


GGGCTCCGACGCCGCCGGCCGGCAGGACGGCCTCCTCTGGTACAAGGACG


 


CCGGGCAGCTCGTCACCGGGGGGTTCTCCATGGCCGTGGTGCAGGCCAAC


 


CAGCTGCTTGAGGACCAGAGCCAGGTGGAGTCCGGATCGCTCTCCCTGGC


 


TGACTACGGCCCGCAGGGCACCTTCGTCGGCGTCTATGATGGCCATGGCG


 


GCCCGGAGACGTCCCGGTTCATCAATGACCACCTCTTCAACCATCTCAGG


 


AGATTCGCAACTGAGCACAAGTCCATGTCAGCAGACGTGATCCGGAAAGC


 


TTTCCAAGAAACTGAGGAGGGCTTTCTTTCTCTAGTCATCAAGGAATGGT


 


CTTTCAAGCCTCAGATTGCATCAGTTGGCTCCTGTTGCCTTGTTGGTGTA


 


ATCTGTGCTGGGACTCTCTATGTTGCAAACCTGGGCGACTCGCGTGCAGT


 


TCTTGGAAGGCTTGTGAAAGCAACTGGAGAGGTTCTGGCCACTCAGTTGT


 


CAGCGGAGCACAATGCATGCTATGAAGAAGTTAGACAAGAGCTGCAGTCA


 


TCACATCCTGATGATCCACGTATTGTGGTTCTCAACATAACGTTTGGCGA


 


GTGAAGGGTCTCATCCAGATCTCAAGATCTATCGGAGATGTATATCTAAA


 


GAAACCGGAGTATAACAGAGAACCTCTTCACAGCAAGTTTCGGCTTCGAG


 


AAACCTTCCAGAAGCCGATTCTTAGTTCTGAACCTCAAATTACTGAACAC


 


CGAATACAGCCAAACGATCAGTTTGTTATATTTGCTTCCGATGGTCTATG


 


GGAGCACCTCAGCAATCAGGAAGCTGTTGACCTTGTCCAAAGTAGTCCCC


 


GTAATGGAATCGCTCGGAGACTAGTGAAAGCCGCGATGCAAGAAGCTGCC


 


AAGAAGAGGGAGATGAGATACTCAGACCTCAAGAAGATCGACCGTGGCGT


 


GAGGAGGCATTTCCACGACGATATAACCGTCGTCGTGGTGTTCCTCGACT


 


CGGATGCCATGAGCAAAGCTAGCTGGAGCAAGAGCCCCTCGTTTTCTCTC


 


CGAGGGGGCGGCGTCACCCTTCCCGCCAAGTCCCTCGCACCCTTCTCGGC


 


TCCGGCACAGTTGAACGGCACCCACTGAAGCTGCTACTGCTCTTGAAAAG


 


AAGGGCACAGTGCAGATCTGCTAGAGATGATGAGAGAAGCAGCAATCAAG


 


TGTAGCTGTTGCTCGTACACCTGCTGTGCTGTTGCTGTTTGCAAAGCTGC


 


CGTCTTGACTCCGCCTGGTAATTAGTGTACTGATAGCGAGGTATAGAAAT


 


TAGGTTATTTGTTAGCGACGCAAATCCTTTCTTTTTTTTTCTTCTCCCTC


 


TGTTCTTATCTCTTTTCTCTTCATCATGGAGGAAACAGGTGGCTGTAAAT


 


TTGTCCAGAACATGTTTTCCCTAATAGCCCAACAAAAAAAAAAA






The ZM62016485 cDNA is translated into the following amino acid sequence (SEQ ID NO:28):








MLAAVMDYFSTCWGPRSRAGHRGKGSDAAGRQDGLLWYKDAGQLVTGGFS


 


MAVVQANQLLEDQSQVESGSLSLADYGPQGTFVGVYDGHGGPETSRFIND


 


HLFNHLRRFATEHKSMSADVIRKAFQETEEGFLSLVIKEWSFKPQIASVG


 


SCCLVGVICAGTLYVANLGDSRAVLGRLVKATGEVLATQLSAEHNACYEE


 


VRQELQSSHPDDPRIVVLKHNVWRVKGLIQISRSIGDVYLKKPEYNREPL


 


HSKFRLRETFQKPILSSEPQITEHRIQPNDQFVIFASDGLWEHLSNQEAV


 


DLVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDIT


 


VVVVFLDSDAMSKASWSKSPSFSLRGGGVTLPAKSLAPFSAPAQLNGTH






cDNA sequence of ZM62051019 from corn (SEQ ID NO:29):








TTTTCTCTTATCCAGCTTCTTAGCATGATTCTCTTTGATCCCGGAGAGCA


 


GCCACCGGTCCAACTAGTCCTTGCTGTTGGTCTGCCGGAACTTTTGATTG


 


CTCTCCAGATCTGAGGCACCTGCTGGGTGGATTCCAGGAACCCGAGCACG


 


AAGTTGACAGGTGAGTTCTCAGGGAAAAAGGGGAGGAAGGAAGAGGCTGA


 


AAGGGCGGTG


 


GAGAGAGAAAGACTATAAGGGCGAGCTGAGTCCCTTGAGGATGCCGTCAA


 


GAAACCGCGTGGAGAGGAGGGCGATGAGATGAGGGATGCTGGCCGCGGTG


 


ATGGACTACTTCAGCTCCTGCTGGGGCCCGCGATCGGGCGCCGGGCACCG


 


GGGCAAGGGCTCCGACGCCGCCGGCCGGCAGGACGGTCTCCTCTGGTACA


 


AGGACGCCGGCCAGCTCGTCACTGGGGAGTTCTCCATGGCCGTGGTGCAG


 


GCCAACCAGCTCCTCGAGGACCAGAGCCAAGTAGAGTCCGGATCGCTCTC


 


CCTGGCTGACCCGGGCCCACAGGGCACCTTCGTCGGCGTCTATGATGGCC


 


ATGGCGGCCCGGAGACGTCCCGGTTCATCAATGACCACCTCTTCAACCAT


 


CTCAGAAGGTTTGCAACTGAGCACAAGTTTATGTCAGCGGACGTGATCCG


 


GAAAGCTTTCCAAGCAACTGAGGAGGGCTTTCTTTCTCTAGTCAGCAAGG


 


AATGGTCTTTGAAGCCTCAGATTGCTTCAGTGGGCTCCTGCTGCCTTGTT


 


GGTGTAATCTGTGCTGGGACTCTCTATGTTGCAAACGTGGGCGACTCACG


 


TGCAGTTCTTGGAAGGCTTGTTAAGGCAACTGGAGAGGTTGTGGCCATGC


 


AGTTGTCATCGGAGCACAATGCGTGCTATGAGGAAGTTAGACAAGAACTG


 


CAGTCATCACATCCTGACGATCCACATATTGTGGTTCTCAAACACAATGT


 


TTGGCGAGTGAAGGGTCTCATCCAGATCTCAAGATCTATTGGAGATGTAT


 


ATCTAAAGAAACCAGAGTACAACAGAGAACCACTTCACAGCAAGTTTCGG


 


CTTCGAGAAACCTTCCAGAGGCCGACCCTTAGTTCTGAACCTCAAATTAC


 


TGAACATCGAATACAGCCGAACGATCAATTTGTTATATTTGCTTCTGATG


 


GTCTATGGGAGCACCTCAGCAATAAGGAAGCAGTTGACCTTGTCCAAAGT


 


AGTCCCCGAAATGGAATCGCTCGGAGGCTAGTGAAAGCCGCGATGCAAGA


 


AGCTGCCAAGAAGAGGGAGATGAGATACTCAGACCTCAAGAAGATCGACC


 


GTGGTGTGAGAAGGCATTTCCACGACGATATAACTGTCGTCGTGGTATTC


 


CTCGATTCGGATGCCATGAGCAAAGCTAGCTGGAGCAAAAGCCCCTCGGT


 


TTCTCTCCGAGGGGGCGGTGTCGCCCTCCCTGCGAAGTCCCTCGCACCTT


 


TCTCAGCTCCGGCACGGCTGAACAGCACCTACTGAAGTTGCTACCACTCT


 


TGAAAGGAAGAACACAGTGCAGATCTGCAGTGGTGAGAGAGAGAGAGAAA


 


ACAGCAAGGAAGTGTAGCGTTACAGTTACACCTGCTGTGTTGTTGCTCTT


 


TGCAAAACTACTGTCTAGACTCCGCCTGGTAATTAGTGTACTGATAGCGA


 


GGTAAAAAAAGTTAGATTATTTGTTAGCGACACACATCCTTTCACCTTCT


 


CTTCTCTCCCTCGATTCCTATCCCTTTTCTCTTCATCCTTGAGAGAACAG


 


GTGGATGTAAATTGTTCAGAACATGTTTTCCCTTATAGTCCATCATATCC


 


CGCTTTTTTCGTGTTGAAAAAAAAAAAAAA






The ZM62051019 cDNA is translated into the following amino acid sequence (SEQ ID NO:30):








MLAAVMDYFSSCWGPRSGAGHRGKGSDAAGRQDGLLWYKDAGQLVTGEFS


 


MAVVQANQLLEDQSQVESGSLSLADPGPQGTFVGVYDGHGGPETSRFIND


 


HLFNHLRRFATEHKFMSADVIRKAFQATEEGFLSLVSKEWSLKPQIASVG


 


SCCLVGVICAGTLYVANVGDSRAVLGRLVKATGEVVAMQLSSEHNACYEE


 


VRQELQSSHPDDPHIVVLKHNVWRVKGLIQISRSIGDVYLKKPEYNREPL


 


HSKFRLRETFQRPTLSSEPQITEHRIQPNDQFVIFASDGLWEHLSNKEAV


 


DLVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDIT


 


VVVVFLDSDAMSKASWSKSPSVSLRGGGVALPAKSLAPFSAPARLNSTY






cDNA sequence of ZM65086957 from corn (SEQ ID NO:31):








CTCTGTCTCCTTGGATTTGCGCTTGTGCTCGTCTGGCCGCATACTAGTAT


 


CCGCACCAGAGAGGAGACACCTCCGACTCCGACCTGCTCTTGCATATAGA


 


TTGGACAGAGAGTGAGGGAGAGAGAGAGCGCGCGCGCTGAAGGGGTGCCA


 


AAGGGAGATTTTTTTTTTTTAATCCAGCTTCTTAGCCTGACTGACTCTCT


 


TTGATCCCGG


 


AGAGCAGCCGCCAGCCCAACTAATCCTTGCTGCTGGCGCGCCGGGGCTCT


 


GATTGCGCTCCAGATCTGAGGCACCTGCTCGGTGGATTCCAGGAATCCGA


 


GCACCAACTCGACAGGGAGAGTTCTCAGGGTAAAGGACGACGCTTGATGC


 


ACACGGGACGGGACAACGAGTTGGCCGCAAGTTTTGTTTGCACACGCACA


 


CGACCCACCAGCTCACGCGTTTTTTTTTTTTTTTTTGCTTCTTAACTCGC


 


TTTGATTGCATCTGTTGTTTCGGAAGGAAGAGGCTGAGAGCGCGTTGGAG


 


ATTTGGACTATAAGAGCGAGCGAGCGAGCGAGCTGGGTGCCTTGAGGACG


 


CCGTCAAGAAACCGCGTGGAGGGGAGGGCGATGAGATGAGGGATGCTGGC


 


CGCGGTGATGGACTACTTCAGCACCTGCTGGGGCCCGCGGTCTCGTGCGG


 


GGCACAGGGGCAAGGGCTCCGACGCCGCCGGCCGGCAGGACGGCCTCCTC


 


TGGTACAAGGACGCCGGGCAGCTCGTCACCGGGGGGTTCTCCATGGCCGT


 


GGTGCAGGCCAACCAGCTGCTTGAGGACCAGAGCCAGGTGGAGTCCGGAT


 


CGCTCTCCCTGGCTGACTACGGCCCGCAGGGCACCTTCGTCGGCGTCTAT


 


GATGGCCATGGCGGCCCGGAGACGTCCCGGTTCATCAATGACCACCTCTT


 


CAACCATCTCAGGAGATTTGCAACTGAGCACAAGTCCATGTCACGAGACG


 


TGATCCGGAAAGCTTTCCAAGAAACTGAGGAGGGCTTTCTTTCTCTAGTC


 


ATCAAGGAATGGTCTTTCAAGCCTCAGATTGCATCAGTTGGCTCCTGTTG


 


CCTTGTTGGTGTAATCTGTGCTGGGACTCTGTATGTTGCAAACCTGGGCG


 


ACTCCCGTGCAGTTCTTGGAAGGCTTGTTAAGGCAACTGGAGAGGTTCTG


 


GCCACGCAGTTGTCAGCGGAGCACAATGCATGCTATGAAGAAGTTAGACA


 


AGAGCTGCAGTCATCACATCCTGATGATCCACGTATTGTGGTTCTAAAAC


 


ATAACGTTTGGCGAGTGAAGGGTCTCATCCAGATCTCAAGATCTATCGGA


 


GATGTATATCTAAAGAAACCGGAGTATAACAGAGAACCTCTTCACAGCAA


 


GTTTCGGCTTCGAGAAACCTTCCAGAAGCCGATTCTTAGTTCTGAACCTC


 


AAATTACTGAACACCGAATACAGCCAAACGATCAGTTTGTTATATTTGCT


 


TCTGATGGTCTATGGGAGCACCTCAGCAATCAGGAAGCTGTTGACCTTGT


 


CCAAAGTAGTCCCCGTAATGGAATCGCTCGGAGACTAGTGAAAGCCGCGA


 


TGCAAGAAGCTGCCAAGAAGAGGGAGATGAGATACTCAGACCTCAAGAAG


 


ATCGACCGTGGCGTGAGGAGGCATTTCCACGACGATATAACCGTCGTCGT


 


GGTGTTCCTCGACTCGGATGCCATGAGCAAAGCTAGCTGGAGCAAGAGCC


 


CCTCGGTTTCTCTCCGAGGGGGCGGCGTCACCCTTCCCGCCAAGTCCCTC


 


GCACCCTTCTCGGCTCCGGCACAGTTGAACGGCACCCACTGAAGCTGCTA


 


CTGCTCTTGAAAAGGGGCACAGTGCAGATCTGCTAGAGATGATGAGAGAA


 


GCAGCAATCAAGTCAAGTGTAGCTGTTGCTCGTACACCTGCTGTGCTGTT


 


GCTGTTTGCAAAGCTGCCGTCTTGACTCCGCCTGGTAATTAGTGTACTGA


 


TAGCGAGGTATAGAAATTAGGTTATTTGTTAGCGACGCAAATCCTTTCTT


 


TTTTTTCTTCTTCTCTCTCTGTTCTTATCCCTTTTCTCTTCATCATGGAG


 


GAAACAGGTGGCTGTAAATTTGTCCAGAACGTGTTTTCCCTAATAGCCCA


 


TCATATCCCGCTATTTTTCTTGTTAAAAAAAAAA






The ZM65086957 cDNA is translated into the following amino acid sequence (SEQ ID NO:32):








MLAAVMDYFSTCWGPRSRAGHRGKGSDAAGRQDGLLWYKDAGQLVTGGFS


 


MAVVQANQLLEDQSQVESGSLSLADYGPQGTFVGVYDGHGGPETSRFIND


 


HLFNHLRRFATEHKSMSADVIRKAFQETEEGFLSLVIKEWSFKPQIASVG


 


SCCLVGVICAGTLYVANLGDSRAVLGRLVKATGEVLATQLSAEHNACYEE


 


VRQELQSSHPDDPRIVVLKHNVWRVKGLIQISRSIGDVYLKKPEYNREPL


 


HSKFRLRETFQKPILSSEPQITEHRIQPNDQFVIFASDGLWEHLSNQEAV


 


DLVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDIT


 


VVVVFLDSDAMSKASWSKSPSVSLRGGGVTLPAKSLAPFSAPAQLNGTH






cDNA sequence of ZM68587657 from corn (SEQ ID NO:33):








GGACGCCGGGCAACATGTCAATGGGGAGTTCTCCATGGCTGTTGTTCAGG


 


CAAATAACTTACTTGAGGACCAGTGTCAGATCGAGTCGGGCCCACTGAGT


 


TTTCTAGATTCTGGACCATATGGCACTTTCGTTGGTGTTTACGATGGGCA


 


TGGTGGTCCAGAGACGGCCTGCTATATCAATGATCATCTTTTCCAGAATC


 


TGAAAAGTAA


 


CTTGCTAACCTTTAAATCTGTGCAGTAGCACTATTCCCGTTTCTTAGCAC


 


TATATCTGCATTTGGCTTTCAGTTTGCACATAAAGGAGATCATCCATTTT


 


TTCATGGCTTGTATTTAGGATTTGCATCTGAGCAGAATGCAATGTCTGCT


 


GATGTACTGAAGAAGGCATATGAAGCTACAGAAGATGGATTCTTCTCCAT


 


TGTTACCAAA


 


CAATGGCCTGTAAAGCCTCAGATAGCAGCTGTCGGCTCATGCTGCCTGGT


 


CGGTGTAATTTGTGGTGGCATGCTTTATGTTGCCAATGTTGGGGATTCCC


 


GTGTCGTTTTAGGAAAACATGTTAAGGCCACTGGAGAAGTTTTGGCTGTC


 


CAACTGTCAGCAGAACATAATGTTAGTATTGCGTCCGTGAGAAAAGAACT


 


GCAGTCAATG


 


CACCCAGAAGATAGGCACATTGTTGTTCTCAAGCACAATGTTTGGCGTGT


 


TAAAGGACTAATTCAGGTTTGTAGATCAATTGGTGATGCATATCTCAAAA


 


AGCAAGAGTTCAACAGGGAACCCCTATATGCAAAATTTCGCCTCCGTGAA


 


CCTTTTCACAAGCCAATACTAAGTTCAGAACCATCAATCAGTGTGCAACC


 


ACTACAACCA


 


CACGACCAGTTTCTCATATTTGCATCTGATGGACTTTGGGAGCAGTTAAC


 


CAACCAAGAGGCAGTTGATATTGTTCGAAGTAGCCCCCGCAGTGGCTGTG


 


CTAGGAGGCTGATAAGAGCGGCACTGCAAGAGGCAGCCAAGAAAAGAGAG


 


ATGAGGTACTCGGACCTCAAGAAGATTGACCGCGGTGTTCGCCGCCACTT


 


CCACGACGACATAACAGTCATAGTAGTGTTCCTTGACTCCGGCCTCGTAA


 


GCCAGGCGAGCACACACCGAGGTCCAACTCTTTCCTTGCGAGGCGGTGGC


 


GGCAGCGCTGGCCTGCGCAGCAACACACTTGCACCTACGTGACTATAAAG


 


TGCCTGGTGGAGTGGAGGCTACTGACTGAAGGTGGTTTTCTTTCCTTGTG


 


TCGAATGTGTTATATATGTACTTGTACCAGCCAAGATCATTCATCCCCCC


 


CCCTAAAATGGTGTAAAGAAGTAGGAGAGGCGCCGAAGTTCCTCACCAGC


 


GTATCTGAATGCCCTCAATGGTGTCAAGTTGTGGACTCAAGTGGATAGCT


 


TCGCTGAATCTTCTGATGATGCTCTGTGGAAAGCTCGAATCCTTTCCACC


 


TGAAAAAGCAAGTAATATGTCTTCCAGTGCTGGAATTAACCCCTAGTGCA


 


TATATATATGTATGAAATAATAATAAGGCAAAAGGAGGAGTAACTTATTT


 


AACTAATGCTGTGAGGTGTATTTATGTTTTGTATGTGTACTGCTTTTGAC


 


TGCTACTGCATCTACTGTTGTTAATTGAAAAAAAAAAAAAAA






The ZM68587657 cDNA is translated into the following amino acid sequence (SEQ ID NO:34):








MSADVLKKAYEATEDGFFSIVTKQWPVKPQIAAVGSCCLVGVICGGMLYV


 


ANVGDSRVVLGKHVKATGEVLAVQLSAEHNVSIASVRKELQSMHPEDRHI


 


VVLKHNVWRVKGLIQVCRSIGDAYLKKQEFNREPLYAKFRLREPFHKPIL


 


SSEPSISVQPLQPHDQFLIFASDGLWEQLTNQEAVDIVRSSPRSGCARRL


 


IRAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITVIVVFLDSGLVSQAS


 


THRGPTLSLRGGGGSAGLRSNTLAPT






cDNA sequence of EST390 from moss (SEQ ID NO:35):








ATCCCGGGTGGAGCCCTTTCAAGCCTCACGCATTCTGGATTCGCTCCCGG


 


CTTCGAATGCTTGAGTGGTTCTAAGTGATGAGATAGCGCCGTCTAGGGAG


 


AATTTCGAATTTGCGCTAGAACATGGGTGGTTATTCCATCAGTGTGGCAG


 


CGCCCACAGATATTGCAGTGAAAGGTTGAACACAACGACCCAAGGACAAC


 


CTGCACCTTCCAACAGTCAGCGTGAGGTGAAAAGATAGGCCAGTTTTCAG


 


CTGCACATAACCTTCACTTCTGCAGGCGCAGAACACGTGCGGTACTGAGC


 


AATGGGGTCCTCTAAGGCAGAAGAGAATTTGGCCTTACGGCTGGGCCTCA


 


CTGCAGCGTCAGCCATGGCGTCGGAGTCTGTGACCTTCCCAATCGATATC


 


ACGAAAACCCGCCTGCAGCTCCAAGGCGAAATGGGTGCCACAGCTGGCGC


 


ACCCAAGCGAGGAGCGATCAGCATGGCGATCTCTATAGGCAAGGAGGAGG


 


GCATTGCCGGTCTTTATAGGGGCCTTTCTCCGGCACTTTTGCGTCATGTA


 


TTTTACACAAGCATTCGTATTGTTGCGTATGAAAATCTACGTACCGCCCT


 


CAGTCATGGCGAACACCCGGAAAATCTGTCCGTTGCAAAAAAGGCTTTCA


 


TCGGTGGCACTTCCGGTATTATTGGGCAGGTGATAGCGAGTCCAGCGGAT


 


TTGGTGAAGGTGCGCATGCAAGCGGATGGGAGGCTGGTGAAGCTTGGGCA


 


GCAGCCACGCTACACCGGAGTAGCTGACGCATTCACCAAGATTGCCCGAG


 


CCGAGGGTGTGACAGGGCTGTGGCGTGGAGTGGGACCCAATGCTCAACGT


 


GCCTTCCTCGTCAACATGGGGGAGCTTGCATGCTACGACCAGTCGAAGCA


 


ATGGATCATAGGACGCGGCATTGCTGCCGACAACATCGGAGCTCACACGC


 


TTGCATCAGTGATGTCTGGGTTATCAGCTACTATTCTGAGCTGCCCTGCC


 


GATGTGGTGAAGACCCGGATGATGAACCAAGGCGCTGCAGGTGCCGTGTA


 


CCGCAACTCTCTGGATTGTCTCACCAAAACCGTGAAGGCTGAAGGCGTGA


 


TGGCGCTGTGGAAGGGCTTCTTCCCGACGTGGACAAGGCTGGGCCCTTGG


 


CAATTCGTGTTTTGGGTCTCATATGAGCAGCTCCGCCGCATCAGCGGTCT


 


ATCATCCTTCTAATAAGTAAAGCCTCGCAGTTGTTTTGGGTGTGAAACTT


 


ACATGGCATTCAGCTCTTACAAAGATTTCACATGCTTGAAGATTTTGAGG


 


TGCTGTTTTTTTTATCATTTTTGTTCCTTCTCTTTTCTGCCTCAATTGGA


 


TGTCATAGCT


 


GAGGCTATGAAGCTTAGTTTCATTGACAAATGTTTACATTTGTTAGCAAT


 


GTGTAGTAGTGCACTTGCGTTAACCG






The EST390 cDNA is translated into the following amino acid sequence (SEQ ID NO:36):








MGSSKAEENLALRLGLTAASAMASESVTFPIDITKTRLQLQGEMGATAGA


 


PKRGAISMAISIGKEEGIAGLYRGLSPALLRHVFYTSIRIVAYENLRTAL


 


SHGEHPENLSVAKKAFIGGTSGIIGQVIASPADLVKVRMQADGRLVKLGQ


 


QPRYTGVADAFTKIARAEGVTGLWRGVGPNAQRAFLVNMGELACYDQSKQ


 


WIIGRGIAADNIGAHTLASVMSGLSATILSCPADVVKTRMMNQGAAGAVY


 


RNSLDCLTKTVKAEGVMALWKGFFPTWTRLGPWQFVFWVSYEQLRRISGL


 


SSF






cDNA sequence of BN51363030 from canola (SEQ ID NO:37):








AGAAAACAAATAAAAATCAAATCGTTACAGCAATGGGCGTCAAAAGTTTC


 


GTGGAAGGTGGGATTGCCCCTGTAGTCGCCGGCTGCTCCACTCACCCTCT


 


CGATCTCATCAAGGTTCGCCTTCAGCTCCACGGCGAAGCTTCCGCCGTCA


 


CTCTCCTCCGCCCAGCTCTCGCTTTCCACAATTCTCCCCCAGCTTTTCTG


 


GAGACGACTC


 


ATTCGGTCCCTAAAGTAGGACCCATCTCCCTCGGAATCAACCTCGTCAAA


 


ACCGAAGGCGCCGCCGCGCTTTTCTCCGGCGTCTCCGCCACACTCCTCCG


 


TCAGACTCTCTACTCCACCACCAGGATGGGTCTCTACGAGGTGTTGAAAA


 


ACAAATGGACTGATCCCGAGTCCGGTAAGCTGAGTCTCACTCGTAAAATC


 


GCCGCGGGGCTAGTCGGTGGCGGGATCGGAGCCGCCGTCGGGAACCCAGC


 


CGACGTGGCGATGGTAAGGATGCAAGCCGACGGGAGGCTTCCCGTGGCAG


 


AGCGTCGTAACTACGCGGGCGTAGGAGACGCGATCAAGAGGATGGCGAAG


 


CAAGAAGGCGTGGTGAGCCTGTGGCGCGGCTCGGCTCTGACGATCAACAG


 


GGCGATGATAGTGACGGCGGCGCAGCTCGCGTCGTACGATCAGTTCAAGG


 


AAGGGATGGTGGAGAGCGGGGGGATGAAAGATGGGCTCGGGACTCACGTG


 


GTGGCGAGCTTCGCGGCGGGGATCGTGGCGGCTGTTGCGTCGAATCCGGT


 


GGATGTGATAAAGACGAGGGTGATGAATATGAAGGTGGATGCGCGTGGTG


 


GGGAGGCTCAGTACAAAGGCGCGTGGGATTGTGCGGTGAAGACGGTTAGA


 


GCTGAAGGACCGATGGCTCTTTATAAAGGGTTTGTTCCTACGGTTTGCAG


 


GCAAGGACCTTTCACTGTTGTGCTCTTTGTTACGTTGGAGCAAGTCAAGA


 


AGCTGCTTCGTGATTTTTGATTATCATTTGAAGGTTATGATGATGAGGAC


 


GACTAAGAATAAGAATGCTAGTAGTATTGATTTGATAGGGATTTTTCGTA


 


TTGGGTTATTCATTTTCG






The BN51363030 cDNA is translated into the following amino acid sequence (SEQ ID NO:38):








MGVKSFVEGGIAPVVAGCSTHPLDLIKVRLQLHGEASAVTLLRPALAFHN


 


SPPAFLETTHSVPKVGPISLGINLVKTEGAAALFSGVSATLLRQTLYSTT


 


RMGLYEVLKNKWTDPESGKLSLTRKIAAGLVGGGIGAAVGNPADVAMVRM


 


QADGRLPVAERRNYAGVGDAIKRMAKQEGVVSLWRGSALTINRAMIVTAA


 


QLASYDQFKEGMVESGGMKDGLGTHVVASFAAGIVAAVASNPVDVIKTRV


 


MNMKVDARGGEAQYKGAWDCAVKTVRAEGPMALYKGFVPTVCRQGPFTVV


 


LFVTLEQVKKLLRDF






cDNA sequence of BN42986056 from canola (SEQ ID NO:39):








TCTAAAAAAACTTTTTGTCTGAACGGCATATGTCTCAGAGACCTCAAGTT


 


CCTCATTCTTCTTCTATAGCTTTCGGTCTCCATTCTCATCTCCTAATCTC


 


CAGTGAGATCAGCTCCAATTCCAACTGGTCTCTCTAAGAAAAAAATAATC


 


AAACCTTTTCAAAATTTTCTCTCGGATTTTCTCGGAATAAAAATCTAACC


 


TTTCTGACTTTTTTGATTTTCGATTTGATAAAAACAAGAAATGGGTCTTA


 


AGGGTTTCGCTGAAGGAGGCATCGCATCGATCGTAGCGGGATGTTCGACC


 


CACCCGCTTGATCTAATCAAGGTCTGAATGCAGCTCCAAGGGGAATCAGC


 


CTCGATTCAGACAAATCTCCGACCAGCTCTTGCTTTCCAGACTTCCTCCG


 


CCGTTCACGCGCCTTCGCCTCCTCCGCGCGTGGGTATAATCACCATCGGA


 


TCTCGCATCATCAGACAAGAAGGCACGTGCACTCTCTTCTCCGGCATCTC


 


CGCCACCTCCGCCACCGTTCTCCGCCAGACTCTCTACTCGACGACTCGCA


 


TGGGTCTATACGACATCCTGAAAACCAAATGGACCGACCCGGAAACCAAA


 


ACCATACCTTTGACCCGCAAACTCGCCGCCGGGTTCATCGCCGGAGGTAT


 


CGGCGCCGCCGTCGGGAACCCGGCGGATGTCGCCATGGTGCGCATGCAAG


 


CCGACGGGAGGCTCCCGGTGGTCGACCGGAGGAACTACAAGAGCGTTTTG


 


GACGCGATCGCGCAGATGGTTCGCGGCGAAGGCGTCACGTCGCTGTGGAG


 


AGGTTCGTCGATGACGATCAACAGAGCGATGCTCGTGACG


 


GCGTCGCAGCTGGCTACGTACGACTCGGTGAAAGAGACGATTTTGGAGAA


 


AGGGTTGATGAGGGACGGGCTCGGGACTCACGTGACGTCGAGCTTCGCGG


 


CGGGGTTTGTGGCTTCGGTCGCGTCGAACCCCGTGGATGTGATCAAGACG


 


AGAGTGATGAATATGAAAGTGGAGGCGGGGAAAACGGCGCCGTATAAGGG


 


AGCGGTTGATTGCGCGTTGAAGACGGTGAGAGCGGAAGGGATCATGGCTT


 


TATACAAAGGGTTTCTGCCGACGGTGTCGAGACAAGCACCGTTCACGGTG


 


ATTATGTTTGTGACACTTGAACAAGTTAAGAAGGTGTTCAAGGACTTTGA


 


CTTTTGAGACAAGAGTTAAAGATGATGGTGGCGATAATTTGCTTTAAACT


 


AAATAAATTTTGTTTTTTTTTATTGTATTTTCTTT






The BN42986056 cDNA is translated into the following amino acid sequence (SEQ ID NO:40):








MQLQGESASIQTNLRPALAFQTSSAVHAPSPPPRVGIITIGSRIIRQEGT


 


CTLFSGISATSATVLRQTLYSTTRMGLYDILKTKWTDPETKTIPLTRKLA


 


AGFIAGGIGAAVGNPADVAMVRMQADGRLPVVDRRNYKSVLDAIAQMVRG


 


EGVTSLWRGSSMTINRAMLVTASQLATYDSVKETILEKGLMRDGLGTHVT


 


SSFAAGFVASVASNPVDVIKTRVMNMKVEAGKTAPYKGAVDCALKTVRAE


 


GIMALYKGFLPTVSRQAPFTVIMFVTLEQVKKVFKDFDF






cDNA sequence of BN49389066 from canola (SEQ ID NO:41):








CGACGATTTCGTTTAATATAAACATCACCAAGTGAATCTCTCCGCCTCTC


 


TCTCTCTTTCTCTGCGGAATCTCTTCGTCTCGTTGCGTTCGAGAGTTCCG


 


TACGATTCCCAACAAGAAAGGGAAGAGATGGCGGAGGAGAAGAAAGTAGC


 


TCCGATTGGTATCTGGACTGCCGTGAAGCCTTTCGTCAATGGCGGTGCCT


 


CTGGTATGCT


 


CGCCACTTGCGTTATCCAGCCTATTGACATGATCAAGGTGAGGATTCAAC


 


TAGGTCAGGGATCTGCAGCTAGTGTGACCACCACCATGTTGAAGAATGAA


 


GGTATCGGTGCCTTCTACAAGGGATTATCAGCTGGTTTGCTGAGGCAAGC


 


AACTTACACCACAGCTCGTCTTGGATCATTCAAGATGCTGACTGCGAAAG


 


CAAGCGAGGCTAACGATGGGAAGCCACTACCGCTGTATCAAAAAGCTCTA


 


TGTGGTCTGACAGCTGGTGCTATCGGTGCCTGCGTTGGTAGTCCAGCCGA


 


TTTAGCGCTTATCAGAATGCAAGCTGATAACACTTTGCCGTTAGCTCAGC


 


GCAGGAACTATACCAACGCCTTCCATGCGCTTTACCGTATTAGCGCTGAT


 


GAAGGAGTTTTGGCGCTTTGGAAAGGTTGTGGGCCAACTGTGGTCAGAGC


 


AATGGCTTTGAACATGGGGATGCTTGCGTCTTATGATCAAAGTGCTGAGT


 


ATATGAGAGATAATCTTGGTCTTGGGGAGACATCCACAGTCGTAGGAGCA


 


AGTGCTGTTTGGGATTCTGCGCTGCGGCTTGCATCTTCCATTTGACTTTG


 


TCAAAACACAGATCCAAAAAATGCAACCCGACGCTCAGGGTAAATATCCA


 


TACACTGGTTCGCAGGACTGTGCGATGCAAAACAGGAGGACCTTTGAAAT


 


TCTACACAGGCTTTCCGGTATACTGCGTCAGGATCGCCCCTCACGTCATG


 


GTGACATGGATCTTCCTGAACCAGATTACAAAGTTCCAAAAGAACATTGG


 


GATGTGATCTTCAAGCAAACCTTATGAAGTGCGCGGTG


 


AAAATATGATGAGAAGAATTCATTTGCTTTTAATCATATACATGATTAG






The BN49389066 cDNA is translated into the following amino acid sequence (SEQ ID NO:42):








MAEEKKVAPIGIWTAVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSA


 


ASVTTTMLKNEGIGAFYKGLSAGLLRQATYTTARLGSFKMLTAKASEAND


 


GKPLPLYQKALCGLTAGAIGACVGSPADLALIRMQADNTLPLAQRRNYTN


 


AFHALYRISADEGVLALWKGCGPTVVRAMALNMGMLASYDQSAEYMRDNL


 


GLGETSTVVGASAVLGFCAAACSLPFDFVKTQIQKMQPDAQGKYPYTGSQ


 


DCAMQNRRTFEILHRLSGILRQDRPSRHGDMDLPEPDYKVPKEHWD-VIF


 


KQTL






cDNA sequence of BN51339479 from canola (SEQ ID NO:43):








CTTTCTCCGCCTATCTCTTTCTCTCCGCGGATTCTCTTCTTCTCGTTTCG


 


ACTCCGTACGATCCCCAAAGAAAAAAAGAGATGGCGGAAGAGAAAAAAGT


 


AGCTCCGATTGGTGTCTGGATACCGTGAAGCCCTTCGTCAATGGCGGTGC


 


CTCCGGTATGCTCGCCACTTGCGTTATCCAGCCGATCGACATGATCAAGG


 


TGAGGATTC


 


AACTAGGTCAGGGATCTGCAGTCAGTGTGACCAAGAACATGTTGAAGAAT


 


GATGGTATTGGTGCTTTCTACAAGGGATTGTCTGCTGGTTTGCTAAGGCA


 


AGCAACTTACACCACAGCCCGTCTTGGATCCTTCAAGATGCTGACTGCAA


 


AGCAATTGAGGCTAACGATGGGAAGCCGCTACCTCTGTACCAGAAGGCTC


 


TATGTGGTC


 


TGACAGCTGGTGCAATCGGTGCTTGCGTTGGTAGTCCAGCTGACTTGGCG


 


CTTATCAGAATGCAAGCTGATAACACCTTGCCGTTAGCTCAGCGCAGGAA


 


CTATACCAATGCCTTCCATGCGCTTTACCGTATTAGCGCTGATGAAGGAG


 


TTTTGGCACTTTGGAAAGGTTGTGGTCCTACTGTGGTCAGAGCTATGGCT


 


TTGAACATGG


 


GAATGCTTGCTTCTTATGATCAAAGTGCTGAGTACATGAGAGATAATCTC


 


GGTCTTGGGGAGACTTCTACGGTCGTAGGAGCAAGTGCTGTTTCTGGATT


 


CTGCGCTGCGGCTTGCAGTCTTCCATTTGACTTTGTCAAAACTCAGATCC


 


AGAAGATGCAACCTGACGCTCAGGGGAAGTATCCATACACGGGTTCGCTT


 


GACTGTGCCA


 


TGCAAACCTTGAAGTCAGGAGGACCTCTTAAATTCTACACAGGTTTCCCT


 


GTTTACTGCGTCAGGATCGCCCCTCACGTCATGATGACATGGATCTTCCT


 


GAACCAGATTACAAAGTTTCAAAAGACCATTGGTCTGTGAGCTTCAAGCA


 


TTGTGAAGTGCGCGCTGAGAATAAGTTGAAAACGAAAACGCAATTGGAAT


 


TGTGTTAGAT


 


TTGCTTTTTATTCAATATACATGATCGCATGCCTTAACGCATTATTTGAA


 


GTGTTGGAGACTTTA






The BN51339479 cDNA is translated into the following amino acid sequence (SEQ ID NO:44):








MAEEKKVAPIGVWNTVKPFVNGGASGMLATCVIQPIDMIKVRIQLGQGSA


 


VSVTKNMLKNDGIGAFYKGLSAGLLRQATYTTARLGSFKMLTAKAIEAND


 


GKPLPLYQKALCGLTAGAIGACVGSPADLALIRMQADNTLPLAQRRNYTN


 


AFHALYRISADEGVLALWKGCGPTVVRAMALNMGMLASYDQSAEYMRDNL


 


GLGETSTVVGASAVSGFCAAACSLPFDFVKTQIQKMQPDAQGKYPYTGSL


 


DCAMQTLKSGGPLKFYTGFPVYCVRIAPHVMMTWIFLNQITKFQKTIGL






cDNA sequence of ZM57651070 from corn (SEQ ID NO:45):








CTAGCACGTGAAAATTCCTTCGGCTCCAGTTATTACGGAGGATTAGGTTG


 


GTGAACTGGTGACTGGAGCTGGAATCGCATTTCTTGCTCTTTGGTCTCTC


 


CAGAATCATCCTCCGGCCAGCCGTTCTTGGAATCCTCCCGAGATTCGCTT


 


GCCCGCCCTTTTCTTTTCAAGTGGATCTGAACTTGGGAGGGAACCCCGAT


 


GCAGCCGCGG


 


TACGGAGAAGCACGACAACCGCTGCCGGGGCGGTACGCGCTGTACCACTT


 


CGGCACCAGCGGCGCCGCCGTCGCCGCCGCCACCGCCGTGACCCATCCGT


 


TCGATGTTATCAAAGTCAGGCTTCAAATGCAGCTTGCTGGGCAAAGAGGA


 


AACTTAGTTGGAATGGGAACAATATTTACACAAATGGTTGAAAGGGAAGG


 


GACTCGGTCACTCTACCTGGGACTTGCACCAGCGTTGGCGAGAGCTGTTG


 


TCTATGGTGGCCTTCGGTTTGGACTGTATGAGCCCTGCAAGCATGTCTGC


 


AGTTATGCATTTGGTTCAACAAACTTTGCTTTTAAATTTGCATCTGGAGT


 


CATTGCTGGGGGCCTTGCAACTGCTTTAACAAATCCCATGGAAGTTTTGA


 


AGGTGAGGCTGCAGATGAGTAAAAGCAGTACCAGTACAATAAGAGAGATG


 


AGAAAAGTTATAGCGCACGAAGGGTTTAAAGCACTTTGGAAAGGAGTCGG


 


CCCAGCAATGACAAGAGCAGGTTGCCTTACTGCATCACAAATGGCGACTT


 


ACGATGAGGCCAAACAGGCCTTAATGAAGTGGACACCACTTGAAGAAGGT


 


TTTCAGTTACATCTCATCTCGAGTTTCATAGCTGGAACAGCTGGTACTCT


 


TGTGACCTCACCTGTAGACATGATCAAAACACGGTTAATGCTGCAACAGG


 


AGTCCAAAGGCGCCAGAGTATACAGGAACGGATTCCATTGTGCTTCCCAG


 


GTTGTGGTGACAGAGGGTGTGAAATCACTTTATAAAGGTGGATTTGCCAC


 


ATTCGCGAGAGTAGGCCCTCAGACAACGATTACCTTTATCGTGTGCGAGA


 


AACTGCGCGAACTTGCAGGAATGACTGCCATCTAGTGCCACCCCAAATTG


 


CATAATGTGTGGGGTCCAACGGTTGAACAGCATACTCTACCCGAGTTTTC


 


ACACCATTCTTTATTCACTATTCATGATGAGAAGGGAGAAGATAAGCACC


 


CACTGGGATGTCTAAGGATTGGGAAGCCCAGAGCTCCTTCAGATTTATCA


 


TACCTCATTTGAAATTTCGAAATAGCGTGATTGTTCTTATGTTTGCTCTA


 


AGACTTACTCATCATATCTCCAATCTCATCTTGTATTTCAAACTACACTC


 


TACAAACAATACAGTCTGTAGTGTAAAAACATTATTTTGGGTGACCATAT


 


GGGTAACCTGCTGTA-CAAAAAAAAAA






The ZM57651070 cDNA is translated into the following amino acid sequence (SEQ ID NO:46):








MQPRYGEARQPLPGRYALYHFGTSGAAVAAATAVTHPFDVIKVRLQMQLA


 


GQRGNLVGMGTIFTQMVEREGTRSLYLGLAPALARAVVYGGLRFGLYEPC


 


KHVCSYAFGSTNFAFKFASGVIAGGLATALTNPMEVLKVRLQMSKSSTST


 


IREMRKVIAHEGFKALWKGVGPAMTRAGCLTASQMATYDEAKQALMKWTP


 


LEEGFQLHLISSFIAGTAGTLVTSPVDMIKTRLMLQQESKGARVYRNGFH


 


CASQVVVTEGVKSLYKGGFATFARVGPQTTITFIVCEKLRELAGMTAI






cDNA sequence of ZM62073276 from corn (SEQ ID NO:47):








GCCGCCTCTCCTACTGCATCTCCCTCGCTCTCGTCGCCTCGTTCGCTTCG


 


CCTCCGCCCCGCCCCGCCCCGAGCAGAGCGCAGCCCTATCCGGAGCTGGG


 


ATGGCGGACGCGAAGCAGCAGCAGCAGCAGCAGCAGCAGCCACAGCAGGC


 


GGCAGCGGCAGCCACCGGCGTGTGGAAGACGGTCAAGCCCTTCGTTAACG


 


GCGAGGCCTCTGGGATGCTCGCGACCTGCGTCATCCAGCCTATCGACATG


 


GTCAAGGTGAGGATCCAGTTGGGTGAGGGCTCTGCTGGTCAGGTCACAAG


 


GAACATGCTTGCAAATGAGGGTGTCCGTTCTTTCTACAAGGGTTTGTCCG


 


CCGGATTGCTGAGGCAAGCGACGTACACGACTGCTCGTCTTGGATCCTTT


 


AGGGTTCTAACTAACAAAGCAGTTGAAAAGAATGAAGGGAAGCCATTGCC


 


TCTATTTCAGAAAGCTTTTATTGGTCTGACTGCTGGTGCAATTGGTGCTT


 


GTGTTGGTAGTCCTGCTGATCTGGCACTCATTAGAATGCAAGCCGATTCG


 


ACCCTGCCAGTTGCACAACGACGCAACTATAAGAATGCTTTCCATGCACT


 


CTACCGTATCAGTGGTGATGAGGGAGTCCTTGCGCTTTGGAAGGGTGCAG


 


GTCCAACTGTGGTGAGAGCTATGGCACTCAATATGGGTATGCTTGCTTCC


 


TATGACCAGAGTGTCGAGCTATTTAGGGACAAATTTGGCGCAGGAGAAAT


 


TTCTACTGTTGTTGGAGCCAGCGCTGTTTCTGGATTCTTTGCCTCAGCAT


 


GCAGTTTGCCCTTTGACTATGTGAAGACACAGATTCAGAA


 


GATGCAACCTGATGCGAATGGCAAGTACCCATACACAGGGTCTTTGGACT


 


GTGCTGTGAAGACCTTCAAGAGCGGTGGCCCATTCAAGTTCTACACTGGT


 


TTCCCGGTGTACTGCGTCAGGATTGCACCCCATGTCATGATGACCTGGAT


 


ATTCTTGAATCAGATCCAGAAGTTTGAGAAGAAGATCGGCATATAGGATT


 


CCCATCGGAC


 


GGATACAGGGTTGACAGTTCTATGCTATTACTGCTTGACTCTGTAATAAC


 


ATTCCAGCTGCTTTCGCACCATGGTAGTTGGTTTTGGTAGAGACAAGTCT


 


GTTACAATTTTTTACCTTAGCTTTCCAATTATTGTGTTGCAATAATCGAA


 


TTAATTGTTGCTGGGGGATTTTTTTGGGGGGTTGGGAGGGTGGCATGCTT


 


TTGTTGGCTG


 


GGATGTAGCCATAAGGAGAGGGGGATACTGCCTAGTTATGTCATTGAATG


 


GAATTGGACCATATTTTATACAGATTTTTACCTTCAAAAAAAAAAAAAA






The ZM62073276 cDNA is translated into the following amino acid sequence (SEQ ID NO:48):








MADAKQQQQQQQQPQQAAAAATGVWKTVKPFVNGEASGMLATCVIQPIDM


 


VKVRIQLGEGSAGQVTRNMLANEGVRSFYKGLSAGLLRQATYTTARLGSF


 


RVLTNKAVEKNEGKPLPLFQKAFIGLTAGAIGACVGSPADLALIRMQADS


 


TLPVAQRRNYKNAFHALYRISGDEGVLALWKGAGPTVVRAMALNMGMLAS


 


YDQSVELFRDKFGAGEISTVVGASAVSGFFASACSLPFDYVKTQIQKMQP


 


DANGKYPYTGSLDCAVKTFKSGGPFKFYTGFPVYCVRIAPHVMMTWIFLN


 


QIQKFEKKIGI






cDNA sequence of EST257 from moss (SEQ ID NO:49):








CCCGGGGATTCAGCAGTACTTCACAAGAAGAATAGCATGGTGCGTGCAGA


 


TCTTGTCAACCTTGCGGACTTAGATACTGCTCTAAACAGAGTTCATAATA


 


AGCTACCTAATTCCATAGAAACAGCTAGTGCAGAGCCTCCTGCTCCTCCA


 


GAAGAATGGGAAATAAATCCTCGAGAGATCACTTTGAAGCATATGATTGC


 


GCGTGGCACCTTTGGGACTGTCCACAAAGGAGTGTACAAAGGTCAGGATG


 


TCGCAGTTAAGCTACTTGAGTGGGGCGAGGAGAATACCATGAAGAAAACA


 


GAGGTTCAATACTACAGAAACCAATTCAGACAAGAGGTTGCTGTGTGGCA


 


TAAACTCGACCACCCTAATGTCACGAAGTTCATCGGAGCCTCGATGGGGA


 


ACTCAGATTTGCGGATTCCCTCAGCCGTGGATGGTGATGATGGATTCCAT


 


CATGTGCCGAACAATGCTTGTTGTGTTGTCGTTGAGTACCTTGCAGGCGG


 


GACTCTTAAAGATCATCTCATTCGCAGCCGGCGGAAAAAACTCTCGTACA


 


AGGTGGTCGTGCAATTAGCCTTGGATGTTTCTAGAGGGCTTGCATACCTC


 


CATTCTCAGAAGATCGCTCATCGTGACGTGAAGACAGAGAACATGTTGCT


 


CGATAAACAGATGAGGGTCAAAATTGCAGATTTCGGAGTTGCACGAGTGG


 


AGGCATCCAATCCCAAGGACATGACTGGTGATACTGGTACCCCAGGATAC


 


ATGGCTCCGGAGATTCTCGACGGCAAGCCCTACAACAAGAAGTGCGATGT


 


GTACAGCTTCGGGATCTGTTTGTGGGAAGTTTATTGCTGCGACATGCCGT


 


ACTTGGACCTCTCCTTTGCGGACATGACATCGGCAGTTGTGCATCAGAAT


 


TTGAGACCCGAGGTGCCCAAGTGCTGCCCTCAGGGACTCGCGGATATCAT


 


GAGGCAGTGTTGGGATGCAAATCCTGAGAAACGGCCTGCCATGGCTGATG


 


TGGTGCAGATGCTGGAGGCTCTAGACACCTCCAAAGGTGGAGGTATGATA


 


CCAACAGACGCCCAGCCGCATGGGTGTCTCTGTTTTGGGAGATTCAAGGG


 


CCCATAGCACGTTTTTGGTTTTTTTTTTCCTTAATTGTGGTTTTACATTT


 


TATTTATATTTTTCCCTTTTTTAATGTAGGGATGACATGATAATAAGTGT


 


GCAAACATTTTGTTGTCTCCCCTGGTTTCGTTTCAAGCGTAGCTGCTTGA


 


CTTGCAATTTCAGTAACCTGGTGCAGGACCCGTTAAC






The EST257 cDNA is translated into the following amino acid sequence (SEQ ID NO:50):








MVRADLVNLADLDTALNRVHNKLPNSIETASAEPPAPPEEWEINPREITL


 


KHMIARGTFGTVHKGVYKGQDVAVKLLEWGEENTMKKTEVQYYRNQFRQE


 


VAVWHKLDHPNVTKFIGASMGNSDLRIPSAVDGDDGFHHVPNNACCVVVE


 


YLAGGTLKDHLIRSRRKKLSYKVVVQLALDVSRGLAYLHSQKIAHRDVKT


 


ENMLLDKQMRVKIADFGVARVEASNPKDMTGDTGTPGYMAPEILDGKPYN


 


KKCDVYSFGICLWEVYCCDMPYLDLSFADMTSAVVHQNLRPEVPKCCPQG


 


LADIMRQCWDANPEKRPAMADVVQMLEALDTSKGGGMIPTDAQPHGCLCF


 


GRFKGP






cDNA sequence of LU61665952 from linseed (SEQ ID NO:51):








AGGGTGATCACGAGGGAGGTATGAATTCTAAGGTGAAGGGAAATGGAAGT


 


GTTAGTAGAAAAGATATGATTTTTCGAGCGGATCGAATCGATTTGAAGAT


 


CCTGGATGTACAGCTAGAGAAGCACCTGAGTAGGGTGTGGTCGAGGAACA


 


CCACAGACAACGCTAAGCCTAAAGAAGAGTGGGAGATTGATTTGTCTAAG


 


TTGGACATCAAAACCCAGATAGCTCGTGGTACTTATGGCACTGTTTATAA


 


AGGCACTTATGATAATCAAGATGTTGCAGTGAAAGTGTTGGATTGGGGGG


 


AAGATGGTATGACTACAGTATCTGAAGCTGCTTCTCTTCGAGCATCATTT


 


CGTCAAGAGGTTGCTGTTTGGCATAAGCTTGACCATCCTAATGTTACCAA


 


ATTCGTTGGAGCATCGATGGGAACTTCAAATCTCAAGGTTTCAAATAATA


 


AATCTGATGGTCAGCATACTGCTAGAGCATGTTGTGTTGTGGTTGAGTAT


 


CAACCTGGTGGAACACTAAAGCAGTACTTGATAAGAAATAGGCGAAAGAA


 


ACTTCCTTATAAAGTTGTAATACAACTTGCTTTGGATCTCTCTAGGGGTT


 


TGAGTTACCTACATTCGAAGAAAATTGTGCACCGTGATGTGAAGTCGGAA


 


AACATGTTGCTTGATAATCATAGAAATCTTAGGATTGCGGATTTTGGTGT


 


TGCTCGAGTCGAAGCTCAAAATCCAAGTGATATGACTGGTGAAACTGGTA


 


CCCTTGGATACATGGCACCTGAGGTCCTTGATGGCAAGCCATATAACAGA


 


AGGTGTGATGTTTATAGCTTCGGCATATGTTTATGGGAAATCTATTGTTG


 


TGATATGCCATATCCAGATCTTAGCTTTGCTGATGTGACGTCCGCGGTTG


 


TTCGACAAAACTTGAGCCGGGAGATTCCGAGATGTTGTCCAAGTTCACTA


 


GGAAGCATCATGAAGAAATGTTGGGATGCACAATCAGAGAACCGTCCAGA


 


AATGGCTGAAGTGGTGAAGATGTTGGAAGCCATTGATACAAGTAAAGGAG


 


GAGGAATGATCCCTGAAGACCAGAACCCTGGTTGTTTCTGCTTCGCCCCA


 


ACCCGTGGCCCTTAAACCCCCTTATTAATTTACTCCCCAAACAGTCCTCA


 


TCCATCTATGTGTGCACAAATTTCAATTTCTTTATATTTGAGTTGTTTTC


 


TTTGTTTATCATTTTCTTGTGTTCTTCACTTCTGCACATATTTTGATTTT


 


GAACTACCTAAAGGGAGTGAAAGGATTAATGTTATAAGTAAAAAAAAAAA


 


AAAA






The LU61665952 cDNA is translated into the following amino acid sequence (SEQ ID NO:52):








MNSKVKGNGSVSRKDMIFRADRIDLKILDVQLEKHLSRVWSRNTTDNAKP


 


KEEWEIDLSKLDIKTQIARGTYGTVYKGTYDNQDVAVKVLDWGEDGMTTV


 


SEAASLRASFRQEVAVWHKLDHPNVTKFVGASMGTSNLKVSNNKSDGQHT


 


ARACCVVVEYQPGGTLKQYLIRNRRKKLPYKVVIQLALDLSRGLSYLHSK


 


KIVHRDVKSENMLLDNHRNLRIADFGVARVEAQNPSDMTGETGTLGYMAP


 


EVLDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFADVTSAVVRQNLRP


 


EIPRCCPSSLGSIMKKCWDAQSENRPEMAEVVKMLEAIDTSKGGGMIPED


 


QNPGCFCFAPTRGP






cDNA sequence of TA56863186 from wheat (SEQ ID NO:53):








AGCACTGACAACTACAACCTCGCTGGTGGCTCCGTTACCATGTCAGTGGA


 


CAACAGCAGCGTGGGCTCGAACGAGTCCCGCACCGTCATACTTAAGCACC


 


CGGGCCTCCGTGATGCTCCAACCGCAAGCTACTCGGTTGGCAACAGTGTC


 


TTTCGTCCCAACCGTGTGGCTGCGCACACCCTAAATGAAGATGCATTGGC


 


CAGGGTTCTGATGGACCCAAATCATCCAACAGAGATACTTAGCAAGTACC


 


AGCAGTGGGCCATTGATCTGGGGAGGTTGGATATGGGGGTTCCCTTTGCA


 


CAGGGAGCCTTTGGGAAGCTGTACCGGGGAACATATATTGGAGAAGATGT


 


TGCCATTAAGCTGCTGGAGAAGCCTGACAATGATATCGAGAGAGCACAAT


 


CGTTGGAACAGCAGTTTGTGCAAGAAGTTATGATGTTATCTACCCTAAGG


 


CACCCAAATATAGTAAGATTTATAGGGGCTTGCAGGAAGTCAATTGTGTG


 


GTGCATTATTACTGAGTATGCAAAAGGTGGCTCAGTCAGGCAGTTCCTGG


 


CAAAAAGGCAGAACAAGTCGGTACCTTTGAGGCTGGCTGTCAAACAAGCA


 


TTGGATGTAGCGAGGGGAATGGCGTATGTGCATGCTCTGGGATTTATCCA


 


TAGGGACCTGAAGTCGGATAATCTTCTAATTGCAGCAGACAGATCCATTA


 


AGATTGCTGACTTTGGAGTTGCTCGAATTGAAGTGAAAACAGAGGGGATG


 


ACACCAGAGACAGGAACCTACCGCTGGATGGCACCGGAAATGATCCAGCA


 


CAGGCCTTATGATCATAAGGTTGATGTCTACAGCTTTGGGATTGTCTTGT


 


GGGAGCTTATAACTGGCATGCTTCCTTTCACAAACATGACAGCTGTTCAG


 


GCGGCTTTTGCTGTTGTAAATAAGGGTGCTCGTCCAGCGATCCCACATGA


 


CTGCCTGCCTTCCCTAACCCACATCATGACGCGCTGTTGGGATGCAAACC


 


CTGAAGTTCGCCCACCATTCACCGAGATCGTCTGCATGCTTGAGAACGCC


 


GAGATGGAGGTCGTGAGCCATGTCCGTAAAGCGCGCTTCCGCTGCTGCGT


 


TGCTGACCCATGACCACCGACTGAAACTAAAGCAGGTTAGACTATCGCAG


 


CGGGCATTAGGGAAGAAAACAGGTAAGGATGAAGAAAAGAGGCAATGCCA


 


ATGTGTTCATCGTTGTCAGTGCGTGGGGTCTGTGTGCCTTTACCAGTGCG


 


CATTCTGTCTTGTGTAAGTTGCACACCTCAAGTAAAAGTAATTTCGTATA


 


GATGTTGCCTTGTATGCTAACAAAGACCTAATGGAGCTTTTCCGTGTTAA


 


TAATATCCGCTTGCTCTTGTACTCGTGCAAGTTTGTGCCAAAAAAAAAAA


 


AAAA






The TA56863186 cDNA is translated into the following amino acid sequence (SEQ ID NO:54):








MSVDNSSVGSNESRTVILKHPGLRDAPTASYSVGNSVFRPNRVAAHTLNE


 


DALARVLMDPNHPTEILSKYQQWAIDLGRLDMGVPFAQGAFGKLYRGTYI


 


GEDVAIKLLEKPDNDIERAQSLEQQFVQEVMMLSTLRHPNIVRFIGACRK


 


SIVWCIITEYAKGGSVRQFLAKRQNKSVPLRLAVKQALDVARGMAYVHAL


 


GFIHRDLKSDNLLIAADRSIKIADFGVARIEVKTEGMTPETGTYRWMAPE


 


MIQHRPYDHKVDVYSFGIVLWELITGMLPFTNMTAVQAAFAVVNKGARPA


 


IPHDCLPSLTHIMTRCWDANPEVRPPFTEIVCMLENAEMEVVSHVRKARF


 


RCCVAEPMTTD






cDNA sequence of ZM62026837 from corn (SEQ ID NO:55):








CGCGCGGCCAAACTCCTGTTCTTCCACCTGCTGGCTGCTCCTGCCTCCCC


 


TGCGCCCCAAACCCACCCGCCTCGCCGTCCCCGCAGGCCGCAGCCTGCTC


 


TCGGCTCCCGCCGCCGTCTACCGCGTCCTGCGGCTGCGGTGTTGCGTCAC


 


CTCGGGTTCGCCTTAACTTCCACAATCCTCGCCGTCCTGGTGCTCCGCCG


 


CCCCTCCCTT


 


TGTACTCGCGCTGGAGCTGCAGATCCACCGCGACCTGGCGACCAATTCCT


 


CCTCCCGCTGAAGAATTGGCGACCTTGGCCTCCGCCCCCGCGGCGCGGAG


 


GAGTCAACTGTGGTAGCAACCACCGCGGAGGCTGCAAGCCTTCGGTAAGG


 


GAGGAAAGTTGACTTGTTGGAAGCCGGTCCAGGGCCGCGATGACGTCGAC


 


CGCCGCCGGCGCGTCGTCGTCGGCGGCGAAGAGCGAGTCCTACCTGCGGG


 


CCGACAAGATCGACCTCGAGAGCCTGGACATCCAGCTGGAGAAGCAGCTG


 


GCCAAGACCTGGGAGAAGCACAAGGGGTCGTACAACCAGGGGCCCAGGGA


 


GGACTGGGAGATCGACCTCGCCAAGCTCGAGATTCGCTACGTCATAGCGC


 


AGGGCACCTACGGCACGGTGTATCGCGGCACGTATGATGGGCAGGACGTC


 


GCAGTAAAACTATTGGATTGGGGTGAAGATGGCTTTGCGTCAGAAACTGA


 


AACTGCCACACTGCGAGCATCATTTAAGCAGGAGGTTGCTGTCTGGCATG


 


AGCTCAACCATCCGAATGTTACAAAGTTTGTTGGTGCATCAATGGGTACT


 


ACAGACCTTAAGATTCCAGCCAATAGTTCTAACAGTGGTGGGCGCACTGA


 


GCTGCCGCCAAAAGCATGTTGTGTTGTGGTCGAATATCTCGCTGGAGGAT


 


CACTGAAGCAGTATTTAATAAAGAACAGGCGAAGGAAGCTTGCATACAAG


 


GTTGTTGTTCAGATAGCACTGGATCTTGCCAGAGGATTGAACTATCTACA


 


TTCAAGAAAGATAGTACATCGGGATGTAAAAACTGAAAATATGCTGCTCG


 


ATACACAGCGAAACCTTAAGATTGCTGATTTTGGTGTTGCTCGTGTTGAG


 


GCTCAGAATCCAAAGGACATGACAGGCGCGACTGGGACACTTGGCTACAT


 


GGCCCCAGAGGTGCTTGAAGGCAAGCCATACAACAGAAAGTGTGATGTCT


 


ACAGTTTTGGCATATGCTTATGGGAAATATACTGCTGTGACATGCCATAT


 


CCAGACCTCAGTTTTGCAGACGTCTCGTCCGCCGTCGTTCACCAGAACCT


 


GCGGCCTGACATCCCTCGCTGCTGCCCAAGCCCAATGGCGAACATCATGC


 


GGAAGTGCTGGGACGCAAACCCGGATAAGCGCCCTGACATGGACGACGTG


 


GTGCGGTTCCTGGAGGCCCTCGACACGAGCAAGGGCGGTGGCATGATACC


 


AGAAGGCCAGGCAGGCGGGTGCTTGTGTTTCTTCAGAGCCCGTGGTCCTT


 


AGAACCAACCAACCCTTTCCAGCCATCCTCTACTTGTCTCTGCCATACTA


 


CAGTATTGGAGCCAGATGTAGGCCTTTGTTGTTCATCGGATAGGGGATTG


 


CAGATAACTTGATGACAATCTTTGTGATTGGTTGACACTTGTTATACGTT


 


CTATAGTGATGTGAATACCAGTGAGGAGTCCATAATACAGAGTGAAAAAA


 


AAAA






The ZM62026837 cDNA is translated into the following amino acid sequence (SEQ ID NO:56):








MTSTAAGASSSAAKSESYLRADKIDLESLDIQLEKQLAKTWEKHKGSYNQ


 


GPREDWEIDLAKLEIRYVIAQGTYGTVYRGTYDGQDVAVKLLDWGEDGFA


 


SETETATLRASFKQEVAVWHELNHPNVTKFVGASMGTTDLKIPANSSNSG


 


GRTELPPKACCVVVEYLAGGSLKQYLIKNRRRKLAYKVVVQIALDLARGL


 


NYLHSRKIVHRDVKTENMLLDTQRNLKIADFGVARVEAQNPKDMTGATGT


 


LGYMAPEVLEGKPYNRKCDVYSFGICLWEIYCCDMPYPDLSFADVSSAVV


 


HQNLRPDIPRCCPSPMANIMRKCWDANPDKRPDMDDVVRFLEALDTSKGG


 


GMIPEGQAGGCLCFFRARGP






cDNA sequence of ZM65457595 from corn (SEQ ID NO:57):








ACCTCGCCACCCTCCTGCCTCCTCCGCATCCGCGCCCCCTCGCTTAGCCT


 


AAACCGCGGGGCAGCTAGTCTCGCCACCGCAGGCCGCACCGGTCATCACA


 


CCGAAGCGCACGCGGGGAGCCCCCGTAGAGTTCCGGGGCGACCAGGCCAA


 


CTAACGCCATGAAGGAGGAAGGCGGCGGCGGGGACGCGGGGTTCGTGCGG


 


GCGGACCAGATCGACCTCAAGAGCCTGGACGAGCAGCTGGAGCGCCATCT


 


CACCCGCGCCTGGACCATGGAGAAGCGCAAGGAGGAGGCCTCCGCCGGCG


 


CTGGCGCCGGCGCCAGGCAGCACCAGCAGTCCCGGCGCCCGCGGAGGGAG


 


GACTGGGAGATCGACCCCGCCAAGCTTGTCGTCAAGGGCGTCATCGCCCG


 


CGGCACCTTTGGCACCGTCCACCGCGGCATCTACGACGCTCACGACGTCG


 


CAGTGAAACTACTTGATTGGGGAGAGGATGGGCATAGATCAGAACAAGAC


 


ATTGCAGCACTAAGAGCAGCTTTTTCACAAGAGGTCTCTGTTTGGCATAA


 


GCTTGACCATCCAAATGTAACCAAGTTTATTGGAGCTATAATGGGTGCAA


 


GGGATCTGAATATTCAAACGGAAAACGGCCACATTGGCATGCCAACTAAT


 


ATCTGCTGTGTCGTTGTGGAGTACCTTCCTGGTGGTGCACTAAAATCATT


 


TCTGATAAAGAACAGGAGAAAGAAGCTAGCTTTTAAGGTCGTTGTTCAAA


 


TCGCTCTTGACCTTGCCAGGGGATTAAGCTATCTCCATTCCAAGAAGATT


 


GTGCACCGTGATGTGAAGACTGAAAATATGCTTCTTGACAAAACGAGAAC


 


CGTGAAGATCGCTGATTTTGGTGTTGCTCGCCTTGAAGCTTCAAATCCCA


 


GTGACATGACGGGCGAAACTGGAACGCTTGGTTACATGACACCTGAGGTT


 


CTCAATGGAAATCCCTACAACAGGAAATGCGATGTTTACAGCTTCGGGAT


 


CTGTTTGTGGGAGATATACTGCTGTGATATGCCATATCCTGACTTGAGCT


 


TTTCTGAGGTCACGTCTGCGGTTGTCCGTCAGAACCTGAGGCCGGAGATA


 


CCACGCTGCTGCCCGAGCTCTCTATCGAACGTGATGAAGCGCTGCTGGGA


 


CGCCAACCCCGACAAGCGACCTGAGATGGCCGAGGCGGTGTCCATGCTGG


 


AGGCGATCGACACGTCGAAGGGTGGAGGCATGATCCCTGTGGACCAGCGG


 


CCAGGATGCCTTGCGTGCTTCCGGCAGTACAGAGGTCCATGACAGATAGG


 


TGGAAACCTGTTGGAGCTGCGGCCTCTAGATCTCGTGGATGCCGATCGAT


 


CGCGTGTTGTTTTCTGGGGAAGCAAACTGGTTAATGGAGCTAGCCCGCCT


 


TACCGGCTCGTGTAAATCCTCTGTCCATCAATTCTGTAACTCTGTTTTAT


 


CGATTAATGAAAAGAACCGGGCTTGCTCGAAAAAAAAAAAAAAA






The ZM65457595 cDNA is translated into the following amino acid sequence (SEQ ID NO:58):








MKEEGGGGDAGFVRADQIDLKSLDEQLERHLTRAWTMEKRKEEASAGAGA


 


GARQHQQSRRPRREDWEIDPAKLVVKGVIARGTFGTVHRGIYDAHDVAVK


 


LLDWGEDGHRSEQDIAALRAAFSQEVSVWHKLDHPNVTKFIGAIMGARDL


 


NIQTENGHIGMPTNICCVVVEYLPGGALKSFLIKNRRKKLAFKVVVQIAL


 


DLARGLSYLHSKKIVHRDVKTENMLLDKTRTVKIADFGVARLEASNPSDM


 


TGETGTLGYMTPEVLNGNPYNRKCDVYSFGICLWEIYCCDMPYPDLSFSE


 


VTSAVVRQNLRPEIPRCCPSSLSNVMKRCWDANPDKRPEMAEAVSMLEAI


 


DTSKGGGMIPVDQRPGCLACFRQYRGP






cDNA sequence of ZM67230154 from corn (SEQ ID NO:59):








CGGCAACCCACTATCTCATGCGCTCACATGGAGACTCCCGCACGAACTGG


 


AATCATCTCCGCCTCGCCACCTCTTCATCTTCTTCCCCAGTAGCCGCCGC


 


CACCACCACTGCAGCAGCCAAACCACGTGACACCTCCCGCGCCGCTCAAC


 


CCCACAGCATCCGTTGCCACCGCCGCTCACCTCCCCGGCGCTCCCGGCTA


 


CAACCACTGC


 


AAGCATGAGGCAGCCAACCAGCGCGGGCGGCGACGCTGGGTTCTTGCGCG


 


CGGACCAGATCGACCTCAAGAGCCTGGACGAGCAGCTCGAGCGCCACCTC


 


GGACATCCCGCGGAGCGGGTAGTTGGCCCAGTCTCTGGGACAGGGAGCCG


 


CCGCGGCGAAACGGCCAAGCTGGGTCCGGAGGAGCTGACGCCACTGCAGC


 


GGTGCCGTGAGGACTGGGAGATCGACCCTACCAAGCTCATCATCAAGGGC


 


GTCATCGCGCGCGGCACCTTTGGCACCGTCCACCGCGGCGTCTACGACGG


 


CCAGGACGTCGCTGTAAAATTGCTTGACTGGGGCGAAGATGGCCATAGAT


 


CAGAACAAGAAATTGGTGCACTAAGAGCAGCGTTTGCACAAGAGGTCGCT


 


GTCTGGCATAAGCTTGAGCATCCAAACGTTACTAAGTTTATTGGGGCTAT


 


AATGGGCGCAAGAGATTTAAATATACAAACGGAACATGGACAGCTTGGCA


 


TGCCAAGCAATATTTGCTGTGTTGTTGTTGAGTACCTTGCTGGAGGTGCG


 


CTGAAAAATTTTCTGATAAAGAACAGGAGAAGGAAACTTGCCTTTAAAGT


 


TGTGGTCCAAATAGCTCTTGACCTTGCCAGGGGATTATGCTACCTCCACT


 


CAAAGAAAATAGTGCACCGTGATGTCAAGACTGAAAACATGCTTCTGGAC


 


AAGACGAGAACGGTAAAGATCGCTGATTTTGGTGTTGCTCGAGTCGAGGC


 


TTCAAATCCTAGCGATATGACGGGAGAAACAGGGACGCTTGGTTACATGG


 


CTCCTGAGGTTCTCAATGGCCATGCTTACAACAGGAAGTGTGACGTGTAC


 


AGCTTTGGGATCTGCCTGTGGGAGATATACTGCTGTGACATGCCGTACCC


 


TGATCTCAGTTTTTCTGAGGTCACCTCTGCCGTCGTTCGCCAGAATCTGA


 


GGCCTGAGATACCGCGCTGCTGCCCGAGCTCGCTAGCGAATGTGATGAAG


 


CGATGCTGGGACGCGAACCCGGACAAGCGTCCCGAGATGGCGGAGGTGGT


 


GTCCATGCTGGAGGCGATCGACACGTCCAAGGGTGGCGGCATGATCCCTA


 


AGGACCAGACGCAGGGCTGCCTCTCGTGCTTCCGCCAGTACCGAGGTCCC


 


TAACGCAGGGTTGTTTATTTATACCCGGTGAAATGATGATATTGGTCTCT


 


ACACTACAACTCAGTGTAATCTAATCGCAGAAGTGGCTATATAATGGAGA


 


AGCTTATCATTGCTTGCCATGGGTGTAAATGGATGGGGCGGGGTGGTTGA


 


CGATTGGTGTGCTTGTATGCTCGCTTCGAGTTATAATGCTTGCTGTAAGT


 


TAAGGTGTGGAAAAAAAAAAAAAA






The ZM67230154 cDNA is translated into the following amino acid sequence (SEQ ID NO:60):








MRQPTSAGGDAGFLRADQIDLKSLDEQLERHLGHPAERVVGPVSGTGSRR


 


GETAKLGPEELTPLQRCREDWEIDPTKLIIKGVIARGTFGTVHRGVYDGQ


 


DVAVKLLDWGEDGHRSEQEIGALRAAFAQEVAVWHKLEHPNVTKFIGAIM


 


GARDLNIQTEHGQLGMPSNICCVVVEYLAGGALKNFLIKNRRRKLAFKVV


 


VQIALDLARGLCYLHSKKIVHRDVKTENMLLDKTRTVKIADFGVARVEAS


 


NPSDMTGETGTLGYMAPEVLNGHAYNRKCDVYSFGICLWEIYCCDMPYPD


 


LSFSEVTSAVVRQNLRPEIPRCCPSSLANVMKRCWDANPDKRPEMAEVVS


 


MLEAIDTSKGGGMIPKDQTQGCLSCFRQYRGP






cDNA sequence of EST465 from moss (SEQ ID NO:61):








GGGCCTCCTTCCTAGCCTTCATCTGCTGCGACGATGGAGGAGCTCGCCTC


 


ATCTGATGTTCCGAACAAGTTGAAGAAGAAGGAATCTAAGATGAAGAAGA


 


GGGTTATAACTCCAGGGGCCTTGCTGAAGGCAGTAGTAAGGTCTGGAGAG


 


GGGACTAAACGTCCTGTAGAAGGTGATCAGATTATCTTCCATTATGTCAC


 


ACGAACAAATCAGGGAGTGGTGGTTGAGACATCGCGATCTGACTTTGGAG


 


GAAAGGGAGTTCCTCTTAGACTTGTTCTGGGAAAAAGCAAAATGATTGCT


 


GGATGGGAGGAAGGCATCACCACCATGGCCAAAGGTGAAATAGCTATGCT


 


GAAAGTGCAACCTGAATTACATTATGGTGACCCGGAGTGTCCTGTACCAG


 


TGCCCGAGAACTTTCCAGTTTCTGATGAGCTCCTTTACGAAGTGGAGTTG


 


TTCAACTTCTGTAAGGCGAAGATTATCACAGAGGATCTTGGTGTGACAAA


 


AGTGGTCTTAGAAGAGGGTGAGGGCTGGGAAACTGCAAGGCCTCCGTACG


 


AGGTGAAGCTTTGGATTACAGGCCGGATCTTAGGTGGGTCCACATTTTTT


 


ACTCATAAAGAGTGCGATCCCATTCATGTTGAATTCGGCAAGGAACAGTT


 


GCCAGAAGGACTTGAGAAGGCAGTCGGCACTATGACGAGGAAAGAAAAGT


 


CAATTATCTACATTTCAAGTTCATACTGTACGAATTCTTCAAATGCATAC


 


AAATTGAATATATCTCCTCAAGCGCAAGAACTAGAATTTGAAGTGCAGTT


 


GGTGCAGCTCATTCAGGTAAGAGACATGTTTGGAGATGGAGGATTGATTA


 


AGAGACGCCTGCGAGACGGACTAGGTGAATTTCCTGTGGACTGTCCTCTG


 


CAAGATAGTGTGCTTAGAGTCCACTATAAGGCTATGCTACCTGATGATGG


 


CGGCAGAATATTTATTGACACCAGAAGTAATGGAGGGGAGCCTGTTGAGT


 


TTGCTTCTGGTGAGGGTGTGGTACCAGAGGGACTTGAGGCAAGTTTGAGG


 


TTGATGCTTCCGGGGGAGCTCGCACTGATCAACAGCGTCTCTAAGTACGC


 


ATATGACAAATTTCAAAGGCCAGAGAGTGTTCCAGAGGGAGCTTCAGTCC


 


AATGGGAAGTGGAATTACTGGAATTTGAGAGTGCAAAGGATTGGACGGGC


 


CTTAATTTTCAAGAGATCATGGCTGAAGCTGATTCCATAAAGACCACAGG


 


TAACCGGTTATTTAAGGAGGGCAAGCACGAGCTGGCTAAAGCTAAGTACG


 


AAAAGGTGTTGAGGGATTTCAGACATGTAAACCCTGGCAGTGATGAAGAA


 


GCAAAGGAACTACAAGACACCAATAACGCACTGCGGCTTAATGTAGCAGC


 


TTGTTATCATAAACTCCATGAGTACATCAAATGCATAGAAACATGCAACA


 


AGGTGCTAGAAGGTAACCCGCATCATGTCAAAGGGTTATTTCGCCGAGGA


 


ACTGCTTACATGGAAACGGGGGACTTTGATGAAGCTAGAGCTGATTTCAA


 


GCAGATGATAACAGTTGACAAGGCTGTCACAGTTGATGCAACTGCTGCTT


 


TACAGAAGCTCAAGCAAAAAGAACGGGAAGCTGAGCTGAAAGCTAAGAAA


 


CAGTTCAAAGGGCTATTTGACTTAAAACCTGGAGAACTCTCTGAGGGGCT


 


AGAAGAGGTAAAGCCCGTAAGCGAAATCCATGAGAAGACTGTTGTCAACG


 


AGGAACTTCCGATAGCATCTATGGATCAACATCAACACTCAAAGCACGAA


 


ACAGAGGAAGGGAGCCATGAATCGCCCAGGGCAAGCAGCCGATTGTTAAG


 


ACTTCTGAAAGGTGGAGAGCACCTGATAAGGACAGTCACTTTTGGGAAGT


 


GTACGATTCTTTAATTTTTCATATTGCTACTGCTAGGATCTCCCCTTTTT


 


ACTGTACTGGTGACTACCTTATGCTCATTTACATTTCTAAGCCGTTATAG


 


CTGTTATTAACCATTCGATAATGTACTATGAACAATATTCCACTAGCGTT


 


TTATGGCTATTTTTCATTAAGTCCTCGTGCCGTTA






The EST465 cDNA is translated into the following amino acid sequence (SEQ ID NO:62):








MEELASSDVPNKLKKKESKMKKRVITPGALLKAVVRSGEGTKRPVEGDQI


 


IFHYVTRTNQGVVVETSRSDFGGKGVPLRLVLGKSKMIAGWEEGITTMAK


 


GEIAMLKVQPELHYGDPECPVPVPENFPVSDELLYEVELFNFCKAKIITE


 


DLGVTKVVLEEGEGWETARPPYEVKLWITGRILGGSTFFTHKECDPIHVE


 


FGKEQLPEGLEKAVGTMTRKEKSIIYISSSYCTNSSNAYKLNISPQAQEL


 


EFEVQLVQLIQVRDMFGDGGLIKRRLRDGLGEFPVDCPLQDSVLRVHYKA


 


MLPDDGGRIFIDTRSNGGEPVEFASGEGVVPEGLEASLRLMLPGELALIN


 


SVSKYAYDKFQRPESVPEGASVQWEVELLEFESAKDWTGLNFQEIMAEAD


 


SIKTTGNRLFKEGKHELAKAKYEKVLRDFRHVNPGSDEEAKELQDTNNAL


 


RLNVAACYHKLHEYIKCIETCNKVLEGNPHHVKGLFRRGTAYMETGDFDE


 


ARADFKQMITVDKAVTVDATAALQKLKQKEREAELKAKKQFKGLFDLKPG


 


ELSEGLEEVKPVSEIHEKTVVNEELPIASMDQHQHSKHETEEGSHESPRA


 


SSRLLRLLKGGEHLIRTVTFGKCTIL






cDNA sequence of YBL109w from yeast (SEQ ID NO:63):








ATGTCCCTACGGCCTTGTCTAACACCATCCAGCATGCAATACAGTGACAT


 


ATATATATACCCTAACACTACCCTAACCCTACCCTATTTCAACCCTTCCA


 


ACCTGTCTCTCAACTTACCCTCACATTACCCTACCTCTCCACTTGTTACC


 


CTGTCCCATTCAACCATACCACTCCCAACCACCATCCATCCCTCTACTTA


 


CTACCACCAATCAACCGTCCACCATAACCGTTACCCTCCAATTAGCCATA


 


TTCAACTTCACTACCACTTACCCTGCCATTACTCTACCATCCACCATCTG


 


CTACTCACCATACTGTTGTTCTACCCTCCATATTAA






The YBL109w cDNA is translated into the following amino acid sequence (SEQ ID NO:64):








MSLRPCLTPSSMQYSDIYIYPNTTLTLPYFNPSNLSLNLPSHYPTSPLVT


 


LSHSTIPLPTTIHPSTYYHQSTVHHNRYPPISHIQLHYHLPCHYSTIHHL


 


LLTILLFYPPY






cDNA sequence of YBL100c from yeast (SEQ ID NO:65):








ATGTTGTTCAAACCAAAAACACGAGCAATACCATCACCGACTGCAAGAAC


 


TCTACCAGTTTCGTTCAAATTGGCCTCGTCGGACACACCCTTAATTCTTT


 


CCTCTAAGATGGAGGAAACTTCTGTGGGTTGTGCCTTGGTGGAAGCCAAT


 


CTTCTGGTGGAAGCCAAAGCAGCAGCGGCAGGTCTTGCGGCCTTGGTAGA


 


GTTAATTAGAGTTCTCGATAGAGAACGAATAGCAGCAGTACGAGCCAACA


 


TTATTATATGTGCGTGTTTTTTTTATTTATTTTGTTACTGTTCTTGCGAT


 


AGTTATGAGAGCTAA






The YBL100c cDNA is translated into the following amino acid sequence (SEQ ID NO:66):








MLFKPKTRAIPSPTARTLPVSFKLASSDTPLILSSKMEETSVGCALVEAN


 


LLVEAKAAAAGLAALVELIRVLDRERIAAVRANIIICACFFYLFCYCSCD


 


SYES






cDNA sequence of YKL184w from yeast (SEQ ID NO:67):








ATGTCTAGTACTCAAGTAGGAAATGCTCTATCTAGTTCCACTACTACTTT


 


AGTGGACTTGTCTAATTCTACGGTTACCCAAAAGAAGCAATATTATAAAG


 


ATGGCGAGACGCTGCACAATCTTTTGCTTGAACTAAAGAATAACCAAGAT


 


TTGGAACTTTTACCGCATGAACAAGCGCATCCTAAAATATTTCAAGCGCT


 


CAAGGCTCGTATTGGTAGAATTAATAATGAAACGTGCGACCCCGGTGAGG


 


AGAACTCGTTTTTCATATGCGATTTGGGAGAAGTCAAGAGATTATTCAAC


 


AACTGGGTGAAGGAGCTTCCTAGAATTAAGCCATTTTATGCCGTCAAATG


 


TAATCCTGATACCAAGGTTTTGTCATTATTAGCAGAGTTGGGCGTTAATT


 


TCGATTGCGCTTCCAAAGTGGAAATTGACAGAGTATTATCGATGAACATC


 


TCGCCGGATAGAATTGTTTACGCTAATCCTTGTAAAGTAGCATCTTTCAT


 


TAGATATGCAGCTTCAAAAAATGTAATGAAGTCTACTTTTGACAATGTAG


 


AAGAATTGCATAAAATCAAAAAGTTTCATCCTGAGTCTCAGTTGTTATTA


 


AGAATCGCTACCGATGACTCTACCGCTCAATGTCGACTTTCCACCAAATA


 


TGGCTGTGAAATGGAAAACGTAGACGTTTTATTAAAGGCTATAAAGGAAC


 


TAGGTTTAAACCTGGCTGGTGTTTCTTTCCACGTCGGTTCAGGCGCTTCT


 


GATTTTACAAGCTTATACAAAGCCGTTAGAGATGCAAGAACGGTATTTGA


 


CAAAGCTGCTAACGAATACGGGTTGCCCCCTTTGAAGATTTTGGATGTAG


 


GTGGTGGATTTCAATTTGAATCCTTCAAAGAATCAACTGCTGTTTTGCGT


 


CTAGCGCTAGAGGAATTTTTCCCTGTAGGTTGTGGTGTTGATATAATTGC


 


AGAGCCTGGCAGATACTTTGTAGCTACAGCGTTCACTTTGGCATCTCATG


 


TGATTGCGAAGAGAAAACTGTCTGAGAATGAAGCAATGATTTACACTAAC


 


GATGGTGTATACGGGAACATGAATTGTATTTTATTCGATCATCAAGAGCC


 


CCATCCAAGAACCCTTTATCATAATTTGGAATTTCATTACGACGATTTTG


 


AATCCACTACTGCGGTCCTCGACTCTATCAACAAAACAAGATCTGAGTAT


 


CCATATAAAGTTTCCATCTGGGGACCCACATGTGATGGTTTGGATTGTAT


 


TGCCAAAGAGTATTACATGAAGCATGATGTTATAGTCGGTGATTGGTTTT


 


ATTTTCCTGCCCTGGGTGCCTACACATCATCGGCGGCTACTCAATTCAAC


 


GGCTTTGAGCAGACTGCGGATATAGTATACATAGACTCTGAACTCGATTA


 


A






The YKL184w cDNA is translated into the following amino acid sequence (SEQ ID NO:68):








MSSTQVGNALSSSTTTLVDLSNSTVTQKKQYYKDGETLHNLLLELKNNQD


 


LELLPHEQAHPKIFQALKARIGRINNETCDPGEENSFFICDLGEVKRLFN


 


NWVKELPRIKPFYAVKCNPDTKVLSLLAELGVNFDCASKVEIDRVLSMNI


 


SPDRIVYANPCKVASFIRYAASKNVMKSTFDNVEELHKIKKFHPESQLLL


 


RIATDDSTAQCRLSTKYGCEMENVDVLLKAIKELGLNLAGVSFHVGSGAS


 


DFTSLYKAVRDARTVFDKAANEYGLPPLKILDVGGGFQFESFKESTAVLR


 


LALEEFFPVGCGVDIIAEPGRYFVATAFTLASHVIAKRKLSENEAMIYTN


 


DGVYGNMNCILFDHQEPHPRTLYHNLEFHYDDFESTTAVLDSINKTRSEY


 


PYKVSIWGPTCDGLDCIAKEYYMKHDVIVGDWFYFPALGAYTSSAATQFN


 


GFEQTADIVYIDSELD






cDNA sequence of YPL091w from yeast (SEQ ID NO:69):








ATGCTTTCTGCAACCAAACAAACATTTAGAAGTCTACAGATAAGAACTAT


 


GTCCACGAACACCAAGCATTACGATTACCTCGTCATCGGGGGTGGCTCAG


 


GGGGTGTTGCTTCCGCAAGAAGAGCTGCATCTTATGGTGCGAAGACATTA


 


CTAGTTGAAGCTAAGGCTCTTGGTGGTACCTGTGTTAACGTGGGTTGTGT


 


TCCGAAGAAAGTCATGTGGTATGCTTCTGACCTCGCTACTAGAGTATCCC


 


ATGCAAATGAATATGGATTATATCAGAATCTTCCATTAGATAAAGAGCAT


 


TTGACTTTTAATTGGCCAGAATTTAAGCAGAAAAGGGATGCTTATGTCCA


 


TAGGTTGAACGGTATATACCAGAAGAATTTAGAAAAAGAAAAAGTGGATG


 


TTGTATTTGGATGGGCTAGATTCAATAAGGACGGTAATGTTGAAGTTCAG


 


AAAAGGGATAATACTACTGAAGTTTACTCCGCTAACCATATTTTAGTTGC


 


GACCGGTGGAAAGGCTATTTTCCCCGAAAACATTCCAGGTTTCGAATTAG


 


GTACTGATTCTGATGGGTTCTTTAGATTGGAAGAACAACCTAAGAAAGTT


 


GTTGTTGTTGGCGCTGGTTATATTGGTATTGAGCTAGCAGGTGTGTTCCA


 


TGGGCTGGGATCCGAAACGCACTTGGTAATTAGAGGTGAAACTGTCTTGA


 


GAAAATTTGATGAATGCATCCAGAACACTATTACTGACCATTACGTAAAG


 


GAAGGCATCAACGTTCATAAACTATCCAAAATTGTTAAGGTGGAGAAAAA


 


TGTAGAAACTGACAAACTGAAAATACATATGAATGACTCAAAGTCCATCG


 


ATGACGTTGACGAATTAATTTGGACAATTGGACGTAAATCCCATCTAGGT


 


ATGGGTTCAGAAAATGTAGGTATAAAGCTGAACTCTCATGACCAAATAAT


 


TGCTGATGAATATCAGAACACCAATGTTCCAAACATTTATTCTCTAGGTG


 


ACGTTGTTGGAAAAGTTGAATTGACACCTGTCGCTATTGCAGCGGGCAGA


 


AAGCTGTCTAATAGACTTTTTGGTCCAGAGAAATTCCGTAATGACAAACT


 


AGATTACGAGAACGTCCCCAGCGTAATTTTCTCACATCCTGAAGCCGGTT


 


CCATTGGTATTTCTGAGAAGGAAGCCATTGAAAAGTACGGTAAGGAGAAT


 


ATAAAGGTCTACAATTCCAAATTTACCGCCATGTACTATGCTATGTTGAG


 


TGAGAAATCACCCACAAGATATAAAATTGTTTGTGCGGGACCAAATGAAA


 


AGGTTGTCGGTCTGCACATTGTTGGTGATTCCTCTGCAGAAATCTTGCAA


 


GGGTTCGGTGTTGCTATAAAGATGGGTGCCACTAAGGCTGATTTCGATAA


 


TTGTGTTGCTATTCATCCGACTAGCGCAGAAGAATTGGTTACTATGAGAT


 


AA






The YPL091w cDNA is translated into the following amino acid sequence (SEQ ID NO:70):








MLSATKQTFRSLQIRTMSTNTKHYDYLVIGGGSGGVASARRAASYGAKTL


 


LVEAKALGGTCVNVGCVPKKVMWYASDLATRVSHANEYGLYQNLPLDKEH


 


LTFNWPEFKQKRDAYVHRLNGIYQKNLEKEKVDVVFGWARFNKDGNVEVQ


 


KRDNTTEVYSANHILVATGGKAIFPENIPGFELGTDSDGFFRLEEQPKKV


 


VVVGAGYIGIELAGVFHGLGSETHLVIRGETVLRKFDECIQNTITDHYVK


 


EGINVHKLSKIVKVEKNVETDKLKIHMNDSKSIDDVDELIWTIGRKSHLG


 


MGSENVGIKLNSHDQIIADEYQNTNVPNIYSLGDVVGKVELTPVAIAAGR


 


KLSNRLFGPEKFRNDKLDYENVPSVIFSHPEAGSIGISEKEAIEKYGKEN


 


IKVYNSKFTAMYYAMLSEKSPTRYKIVCAGPNEKVVGLHIVGDSSAEILQ


 


GFGVAIKMGATKADFDNCVAIHPTSAEELVTMR






cDNA sequence of TA54587433 from wheat (SEQ ID NO:71):








ATGGCGGTCATGTCACGGTTGAAGAGGCTGGCGGCGCCCGCGCTGCTGGT


 


GCTGCTTGCGCTGGCGGCGTCCGCGGCCGTGGCGGCGAAGACGACCCAGG


 


ACGGCGCGGAGGCGGCGCCGGGCAAGGATGAAGAGTCGTGGACGGGGTGG


 


GCCAAGGACAAGATCTCCGAGGGGCTGGGGCTCAAGCACGACGCTGACGA


 


GGAGGCCGCGCGCGAGACCGTCCAGCACACCGCCTCCGAGACGGGGAGTC


 


AGGTGAGCGGCAAGGCAGCGGACGCCAAGGAGGCGGCCAAGGGAACGGTC


 


GGGGAGAAGCTCGGGGAGGTGAAGGACAAGGTCACCGGCGCAGCAGCCGA


 


CGGCAAGGACAAGACGCACCGCAAGGATGACTTGCTGTGA






The TA54587433 cDNA is translated into the following amino acid sequence (SEQ ID NO:72):








MAVMSRLKRLAAPALLVLLALAASAAVAAKTTQDGAEAAPGKDEESWTGW


 


AKDKISEGLGLKHDADEEAARETVQHTASETGSQVSGKAADAKEAAKGTV


 


GEKLGEVKDKVTGAAADGKDKTHRKDDLL






cDNA sequence of ZM68532504 from corn (SEQ ID NO:73):








ATGCCGTCGCACGGGGATCTGGACCGGCAGATCGCGCAGCTGCGCGACTG


 


CAAGTACCTGCCCGAGGCGGAGGTCAAGGCGCTCTGCGAGCAGGCCAAGG


 


CCATCCTTATGGAGGAGTGGAACGTGCAGCCCGTGCGCTGTCCTGTCACC


 


GTCTGTGGCGACATCCACGGCCAGTTCTATGACCTCATCGAGCTCTTCCG


 


CATCGGCGGCGACGCTCCCGACACCAACTACCTCTTCATGGGCGACTACG


 


TCGATCGTGGGTACTATTCAGTTGAAACAGTTTCTCTGTTAGTGGCTTTG


 


AAAGTCCGTTACAGAGATAGAATTACAATACTTAGAGGAAATCATGAGAG


 


CAGACAAATCACTCAAGTATATGGCTTCTATGATGAATGCTTAAGAAAGT


 


ATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTG


 


CCTCTCACAGCTCTTATAGAAAATCAGGTCTTCTGTCTTCACGGTGGCCT


 


CTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTAC


 


AGGAGGTTCCTCATGAAGGACCCATGTGTGATCTTTTGTGGTCTGACCCA


 


GATGACCGATGTGGATGGGGAATTTCACCAAGAGGAGCAGGTTACACATT


 


TGGGCAAGACATTGCGCAGCAGTTCAACCATACAAATGGTCTTTCTCTCA


 


TTTCAAGGGCCCATCAACTTGTAATGGAAGGATTTAATTGGTGCCAGGAT


 


AAGAATGTAGTCACAGTCTTCAGCGCGCCTAATTATTGTTACCGCTGTGG


 


TAACATGGCTGCTATTCTTGAAATCGGGGAAAACATGGACCAGAACTTCC


 


TTCAATTCGACCCGGCACCTCGGCAAATTGAGCCAGACACAACTCGGAAA


 


ACCCCAGACTACTTTTTGTAA






The ZM68532504 cDNA is translated into the following amino acid sequence (SEQ ID NO:74):








MPSHGDLDRQIAQLRDCKYLPEAEVKALCEQAKAILMEEWNVQPVRCPVT


 


VCGDIHGQFYDLIELFRIGGDAPDTNYLFMGDYVDRGYYSVETVSLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDFL


 


PLTALIENQVFCLHGGLSPSLDTLDNIRSLDRVQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEPDTTRK


 


TPDYFL






cDNA sequence of BN42856089 from canola (SEQ ID NO:75):








AAAACTCCAAAAACAAACCATTTTCCATCTCTCAGGCCAAAAAAACCAGA


 


GATTTGATCTCTCTGGCGATTCATCATCCTCTTCATCCACCACACGCCGT


 


ATAAGTTAAAGGATCGGTGGTGGTCTCTCGATGCCGCCGAACGGAGATCT


 


AGACCGTCAGATCTCCCAGCTGATGGAGTGTAAACCGCTATCTGAGGCCG


 


ATGTGAAGACGCTCTGCGATCAAGCGAGGGCCATCCTCGTCGAGGAGTGG


 


AACGTTCAGCCCGTGAAGTGTCCTGTCACCGTCTGCGGCGATATCCACGG


 


ACAGTTCTATGACCTTATCGAGCTCTTTCGAATCGGTGGGAATCCTCCGG


 


ATACTAACTACCTCTTCATGGGAGACTATGTAGACCGTGGCTACTATTCA


 


GTAGAAACAGTTTCTCTATTGGTGGCACTGAAAGTGCGATACAGGGATAG


 


GATTACAATCTTGCGAGGGAATCACGAGAGTCGGCAGATTACTCAAGTCT


 


ATGGGTTTTATGATGAATGTTTGAGGAAGTATGGAAATGCAAATGTCTGG


 


AAGTTTTTCACGGACCTTTTCGATTATCTTCCTCTTACTGCTCTCATAGA


 


GAGTCAGGTTTTCTGCTTGCATGGAGGGCTTTCACCTTCTCTGGACACCC


 


TTGATAATATCCGAAGCTTGGATCGTATACAAGAGGTTCCACATGAAGGA


 


CCAATGTGTGATTTATTATGGTCTGATCCCGATGATCGATGTGGGTGGGG


 


AATATCTCCACGAGGTGCTGGTTATACATTTGGACAAGACATCGCAACTC


 


AGTTTAATCACAACAATGGACTCAGTCTCATATCAAGAGCACATCAACTT


 


GTCATGGAAGGCTTTAACTGGTGTCAGGACAAAAATGTTGTGACGGTGTT


 


TAGTGCACCAAACTATTGCTACCGGTGTGGAAACATGGCAGCTATTCTAG


 


AGATAGGAGAGAACATGGACCAGAACTTCCTCCAGTTCGATCCAGCTCCT


 


CGTCAAGTCGAACCAGATACTACCCGCAAGACCCCTGATTATTTTTTGTG


 


ATTTATTTGCATTTTTTTTTCTTTTGTTCCCAACCATTTATAATTTTTAA


 


AAAATCTGTTTTATCTTGCTTATGAATAATCATTCTAGTGTCTCTTCAAA


 


AAAAAAAAAAAA






The BN42856089 cDNA is translated into the following amino acid sequence (SEQ ID NO:76):








MPPNGDLDRQISQLMECKPLSEADVKTLCDQARAILVEEWNVQPVKCPVT


 


VCGDIHGQFYDLIELFRIGGNPPDTNYLFMGDYVDRGYYSVETVSLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKFFTDLFDYL


 


PLTALIESQVFCLHGGLSPSLDTLDNIRSLDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIATQFNHNNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQVEPDTTRK


 


T-PDYFL






cDNA sequence of BN43206527 from canola (SEQ ID NO:77):








CCAAAGACCATTTGATCTCTGGCGATTTCATCTTCCGATATGCCGCCGAA


 


CGGAGATCTAGACCGTCAGATCGAGCATCTGATGGAGTGCAAACCTTTAT


 


CGGAGGAGGATGTGAGGACGCTCTGCGATCAAGCTAAGGCCATCCTCGTC


 


GAGGAATGGAACGTCCAGCCCGTGAAATGCCCCGTCACCGTCTGCGGCGA


 


TATCCACGGCCAGTTCTATGACCTTATCGAGCTTTTCCGAATCGGTGGTA


 


ACGCCCCCGATACGAATTACCTCTTCATGGGTGACTATGTAGACCGTGGC


 


TACTATTCAGTGGAAACGGTTTCTTTATTGGTGGCATTGAAAGTCAGATA


 


CAGGGATAGGATTACAATCTTGCGAGGGAACCACGAGAGTCGTCAGATCA


 


CCCAAGTATATGGTTTTTATGACGAGTGCTTGAGGAAGTACGGAAACGCA


 


AATGTGTGGAAGTATTTCACAGACCTTTTCGATTATCTTCCTCTTACTGC


 


TCTTATCGAGAGTCAGGTTTTCTGTTTGCATGGAGGGCTATCACCTTCTC


 


TGGATACACTTGATAATATCCGAAGCTTGGATCGTATACAAGAGGTTCCA


 


CACGAAGGACCAATGTGTGATTTACTATGGTCTGATCCAGATGATCGATG


 


CGGGTGGGGAATATCTCCAAGAGGTGCTGGTTATACATTTGGACAGGATA


 


TAGCAACTCAGTTTAATCACAACAATGGACTCAGTCTCATATCAAGAGCG


 


CATCAGCTTGTCATGGAAGGTTTTAACTGGTGTCAGGATAAGAATGTGGT


 


GACGGTGTTTAGTGCACCAAACTATTGCTACCGGTGTGGAAACATGGCAG


 


CGATTCTAGAGATAAGTGAGAACATGGAGCAGAACTTCCTTCAGTTTGAT


 


CCAGCTCCAAGACAAGTCGAACCTGATACTACCCGTAAGACCCCTGATTA


 


TTTTTTGTGATTTTATTTGTATTTTTTTTTCTTCTAAGCGGAGTTCGAGT


 


TTCCCTCAAAACGAAAGAAAGAAACAAACATCATTTTGTTGTTGTTGATG


 


TGATTGCTGAGAACAAAGTTTGTAGTAGAAGCGTCTATATATAGAATAGT


 


GTCTTCTCATTGTCATTTCACTTGTTACTGCATAGAGGAATGAGGTTTCG


 


AACCCTGCCCGCCACTTTCATTTCAGTTTCATTTATAAAATATGAGTTTG


 


ATACCGAAAAAAAAAAAAAAA






The BN43206527 cDNA is translated into the following amino acid sequence (SEQ ID NO:78):








MPPNGDLDRQIEHLMECKPLSEEDVRTLCDQAKAILVEEWNVQPVKCPVT


 


VCGDIHGQFYDLIELFRIGGNAPDTNYLFMGDYVDRGYYSVETVSLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYL


 


PLTALIESQVFCLHGGLSPSLDTLDNIRSLDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIATQFNHNNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEISENMEQNFLQFDPAPRQVEPDTTRK


 


T-PDYFL






cDNA sequence of HA66872964 from sunflower (SEQ ID NO:79):








CTAAAAATATCTTTAACCGCCGGCTGCCATGACGGAACCCTAAGCAACTT


 


CTCCGGCGACTCCGGCGGAGCTCCGTTCAACCTAAATGCGAATCATTCTT


 


CCAGATCTTCAAATCCGAACACACAAATCACGTAACAATGCCGTCGCAAT


 


CGGATCTGGACCGTCAGATCGAGCACTTGATGGACTGTAAACCGCTGCCG


 


GAGGCGGAGGTGCGGACGTTGTGTGATCAGGCGAGGACGATTTTGGTCGA


 


GGAGTGGAATGTGCAGCCGGTGAAGTGTCCGGTGACTGTTTGCGGTGATA


 


TTCATGGGCAGTTTCATGATTTGCTTGAGCTGTTTCGGATCGGAGGAAGT


 


GCTCCGGACACGAATTACTTGTTTATGGGAGATTATGTTGATCGAGGCTA


 


TTACTCGGTGGAGACTGTTACGCTTCTTGTGGCATTGAAAGTTCGTTACA


 


GAGATAGGATTACTATTCTCAGAGGAAACCATGAGAGCAGGCAGATAACT


 


CAAGTGTATGGATTTTACGATGAATGCTTGAGGAAGTACGGAAACGCAAA


 


TGTATGGAAACATTTCACTGACCTTTTTGATTATCTACCTCTCACTGCCC


 


TTATCGAGAGTCAGATATTCTGTCTCCATGGTGGCTTGTCTCCATCTTTG


 


GATACACTAGATAACATACGTGCTTTAGATCGCATACAAGAGGTTCCTCA


 


TGAGGGGCCAATGTGTGACCTTTTGTGGTCTGATCCTGATGACCGGTGTG


 


GTTGGGGAATATCTCCTCGTGGAGCCGGTTACACTTTCGGGCAGGATATA


 


GCCGCACAGTTTAACCATACAAACGGGCTCTCGCTTATTTCTCGGGCTCA


 


CCAGCTTGTCATGGAAGGTTACAATTGGTCTCAGGAGAACAACGTTGTAA


 


CCATATTTAGTGCACCAAACTACTGCTATAGATGCGGGAATATGGCTGCG


 


ATACTTGAGGTTGGAGAGAATATGGACCAGAATTTCTTACAATTTGACCC


 


AGCCCCTCGTCAGGTTGAGCCCGATGTTGCACGAAGAACTCCGGATTACT


 


TCCTGTAAATTTGTGTTGGATAATATGACCTTTGCATGCATCCTATTTAT


 


GTTGTTATAGTTTTCGCTTTCCCCTGCTAGAGAGTCCCCCTATTCTTGAG


 


AATTAAAGACAATATGTATGATTGTTTGTCCCTTGTTCTATTTGAGATTA


 


TTTGTTTAAAAAAAAAAAAAAA






The HA66872964 cDNA is translated into the following amino acid sequence (SEQ ID NO:80):








MPSQSDLDRQIEHLMDCKPLPEAEVRTLCDQARTILVEEWNVQPVKCPVT


 


VCGDIHGQFHDLLELFRIGGSAPDTNYLFMGDYVDRGYYSVETVTLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKHFTDLFDYL


 


PLTALIESQIFCLHGGLSPSLDTLDNIRALDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIAAQFNHTNGLSLISRAHQLVMEGYNWSQE


 


NNVVTIFSAPNYCYRCGNMAAILEVGENMDQNFLQFDPAPRQVEPDVARR


 


T-PDYFL






cDNA sequence of LU61662612 from linseed (SEQ ID NO:81):








CATCTCTCTTTCTCTCTCTTCCATTTTCGTTCTTTTGAATCTCCGTTAGC


 


CCTACAAATCCATGGTCATGGCCTGAGAGAGATAGAGGGATAGAGCTCTC


 


AGTTCCTAATCACCTTACCTGACCTAACCCCACGGACATATTATCGAAGG


 


TCTGCGAGCAGGAGAGCGCAGGAGGAAGAGTGGGGCCAGGGTACGATGCC


 


GTCCCACGCCGATCTGGACCGTCAGATCGAGCACTTGATGCAGTGCAAGC


 


CACTTTCTGAGGCCGAAGTGAAGGCTCTCTGCGAGCAGGCCAGGGCCGTC


 


CTCGTCGAGGAATGGAACGTCCAGCCGGTCAAGTGTCCGGTGACTGTCTG


 


CGGCGACATCCACGGCCAGTTTCACGATCTTGTCGAGCTCTTTCGAATCG


 


GAGGAAACGCCCCTGACACGAACTACCTCTTCATGGGCGACTATGTAGAT


 


CGAGGGTATTATTCGGTGGAGACTGTCACCCTTCTAGTCGCCTTGAAAGT


 


AAGATATAGAGATAGGATCACAATTCTGAGAGGAAATCATGAAAGTCGTC


 


AAATAACTCAAGTGTATGGATTCTATGATGAGTGCTTGAGAAAATATGGA


 


AATGCCAATGTGTGGAAACATTTTACCGATCTCTTTGATTATCTACCACT


 


TACAGCTCTGATTGAGAGTCAGGTCTTCTGCTTACATGGTGGACTTTCCC


 


CTTCACTAGACACGCTAGACAACATTCGCTCCCTTGATCGTATCCAAGAG


 


GTTCCGCACGAGGGTCCTATGTGCGACCTCCTATGGTCGGACCCGGATGA


 


CCGTTGCGGGTGGGGGATCTCTCCTCGTGGAGCTGGCTACACCTTTGCAC


 


AGGACATATCTCAACAGTTCAACCACACGAACGGCCTTTCTCTCATATCC


 


AGAGCTCACCAGCTGGTCATGGAAGGTTACAATTGGGCCCAGGACAAGAA


 


TGTGGTGACGGTGTTCAGCGCCCCGAACTACTGCTACCGGTGTGGGAACA


 


TGGCGGCCATTCTCGAGATCGGAGAGAACATGGAGCAGAACTTCCTGCAG


 


TTCGACCCAGCTCCTCGACAGATCGAACCGGAGACGACTCGCAGAACACC


 


CGATTATTTTTTGTGAAATGCATAGCTTCTTCTTCCTCCCTCCTTCTTGC


 


TTGGAAATGGGATCCGTGTCCATTTTTTCTAATCGCCTGCCCTGCTATGT


 


GCTTATGTTTTTTGTAGATGCATTCATCATCATCATATCCAGAATAGAGA


 


AGAAATTTTGGTGTTTGCTTTGATTGAGAAAAGGCGGGGAGGGAAAAATC


 


GGCCTCTAGAGATGCTGGGTGTTGTCATTTTTCTTCTTCTTCTTCCTCCT


 


TTTGGGATGGTTTCGTTTTTACTTTTTCTTTTGGGTTTCTATTGTTTATC


 


CTGCATTCATTTGAGTTTAACAAAGTTTATTATTTACAGTCTGGGTGTGT


 


TATTAATATTATTCACTGTGGTCTTGTACCAAAAAAAAAAAAAAA






The LU61662612 cDNA is translated into the following amino acid sequence (SEQ ID NO:82):








MPSHADLDRQIEHLMQCKPLSEAEVKALCEQARAVLVEEWNVQPVKCPVT


 


VCGDIHGQFHDLVELFRIGGNAPDTNYLFMGDYVDRGYYSVETVTLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKHFTDLFDYL


 


PLTALIESQVFCLHGGLSPSLDTLDNIRSLDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDISQQFNHTNGLSLISRAHQLVMEGYNWAQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGENMEQNFLQFDPAPRQIEPETTRR


 


TPDYFL






cDNA sequence of OS32806943 from rice (SEQ ID NO:83):








GAGGCTTGAGCTCCACCTCCACCTCCTCCACCTCCAACCCCCGATCCCCC


 


GCAAACCCTAGCCCTCTCCCCCACCCTCCTCGCCGGCGGCGAGCGGCGGC


 


GGCGCGCGGCGGGACCCGGAGCCCCCAGTAGCGCCTCCTCGTCCTCCCCT


 


CCCTGAGGTGCGGGGGAGAGGATGCCGTCGTCGCACGGGGATCTGGACCG


 


GCAGATCGCGCAGCTGCGGGAGTGCAAGCACCTGGCGGAGGGGGAGGTGA


 


GGGCGCTGTGCGAGCAGGCGAAGGCCATCCTCATGGAGGAGTGGAACGTG


 


CAGCCGGTGCGGTGCCCCGTCACGGTCTGCGGCGACATCCACGGCCAGTT


 


CTACGACCTCATCGAGCTCTTCCGCATCGGCGGCGAGGCGCCCGACACCA


 


ACTACCTCTTCATGGGCGACTACGTCGACCGTGGCTACTACTCAGTGGAG


 


ACTGTTTCGTTGTTGGTGGCTTTGAAAGTACGCTACAGAGATCGAATTAC


 


AATATTGAGAGGAAATCATGAGAGCAGACAAATCACTCAAGTGTACGGCT


 


TCTACGATGAATGCTTGAGAAAGTATGGAAATGCAAATGTATGGAAATAC


 


TTTACAGACTTGTTTGATTATTTGCCTCTCACAGCTCTTATAGAAAACCA


 


GGTGTTCTGCCTTCACGGTGGTCTCTCTCCATCATTGGATACTTTAGATA


 


ACATCCGTGCTCTTGATCGTATACAAGAGGTTCCTCATGAAGGACCCATG


 


TGTGATCTTTTGTGGTCTGACCCAGATGACAGATGCGGGTGGGGAATTTC


 


ACCGAGAGGAGCAGGTTATACATTTGGGCAAGATATCGCTCAACAGTTTA


 


ACCATACAAATGGTCTATCTCTCATCTCAAGGGCACATCAACTTGTAATG


 


GAAGGATTTAATTGGTGTCAGGACAAGAATGTTGTGACGGTCTTCAGTGC


 


ACCAAACTACTGTTATCGCTGTGGTAACATGGCTGCAATTCTTGAGATTG


 


GCGAAAACATGGATCAGAACTTCCTCCAATTTGATCCAGCTCCTCGGCAA


 


ATTGAACCAGACACAACACGCAAGACTCCCGACTACTTTTTGTAATTTGT


 


GGTGTTGACAATTTTAACTCACCTGTGTTGATGCTCCTCTCCTCCGCGGT


 


GTCGGGGTCTGTAGATCTTCTGTCCTTAGATACGGGTTCCACGAGCCCGG


 


CTGTATGTCTCTCAATTCTTTTGTTTGGAGATTTTGTTGCTGCTTCTCAA


 


CCTTTATACAAGACGTTAAAAGTTACATGCACTGGATTTTTTTCTCAAAA


 


AAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAA






The OS32806943 cDNA is translated into the following amino acid sequence (SEQ ID NO:84):








MPSSHGDLDRQIAQLRECKHLAEGEVRALCEQAKAILMEEWNVQPVRCPV


 


TVCGDIHGQFYDLIELFRIGGEAPDTNYLFMGDYVDRGYYSVETVSLLVA


 


LKVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDY


 


LPLTALIENQVFCLHGGLSPSLDTLDNIRALDRIQEVPHEGPMCDLLWSD


 


PDDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVMEGFNWCQ


 


DKNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEPDTTR


 


KTPDYFL






cDNA sequence of OS34738749 from rice (SEQ ID NO:85):








GGTCGACGCCGTCACCGTCGCGCCAACTGCCGCAAACCGAATAAACCGAA


 


TCGATCTGAGAGAAGAAGAAGAAGAAGACGCGATCTCGGAGGTGGGAGCG


 


AAACGAAACGATGCCGTCTCACGCGGATGTGGAACGACAGATCGAGCAGC


 


TGATGGAGTGCAAGCCTCTGTCGGAGTCGGAGGTGAAGGCGCTGTGTGAT


 


CAAGCGAGGGCGATTCTCGTGGAGGAATGGAACGTGCAACCGGTGAAGTG


 


CCCCGTCACCGTCTGCGGCGATATTCACGGCCAGTTTTACGATCTCATCG


 


AGCTGTTTCGGATTGGAGGGAACGCACCCGATACCAATTATCTCTTCATG


 


GGTGATTATGTAGATCGTGGATACTATTCAGTGGAGACTGTTACACTTTT


 


GGTGGCTTTGAAAGTCCGTTACAGAGATAGAATCACAATTCTCAGGGGAA


 


ATCATGAAAGTCGTCAAATTACTCAAGTGTATGGCTTCTATGATGAATGC


 


TTGAGAAAATATGGAAATGCCAATGTCTGGAAATACTTTACAGACTTGTT


 


TGATTATTTACCTCTGACTGCCCTCATTGAGAGTCAGATTTTCTGCTTGC


 


ATGGAGGTCTCTCACCTTCTTTGGATACACTGGATAACATCAGAGCATTG


 


GATCGTATACAAGAGGTTCCACATGAAGGACCAATGTGTGATCTCTTGTG


 


GTCTGACCCTGATGATCGCTGTGGATGGGGAATATCTCCACGTGGTGCAG


 


GATACACATTTGGACAGGATATAGCTGCTCAGTTTAATCATACCAATGGT


 


CTCTCCCTGATATCGAGAGCTCATCAGCTTGTTATGGAAGGATTCAATTG


 


GTGCCAGGACAAAAATGTGGTGACTGTATTTAGTGCACCAAATTACTGTT


 


ACCGATGTGGGAATATGGCTGCTATACTAGAAATAGGAGAGAATATGGAT


 


CAGAATTTCCTTCAGTTTGATCCAGCGCCCAGGCAAATTGAGCCTGACAC


 


CACACGCAAGACTCCAGATTATTTTTTATAATTTCATTTATCTGCCTGTT


 


TGTAGTTACTGCTCTCTGCCATTACTGTAGATGTGTCTTTAAGGAAAGGA


 


GTTTTGCTGTTTAAGTGGAGGGTGGTCATCAACATAATTCTTTCTTTTGG


 


AGTTTACCTCCTGCTGCTGCCGCTGCCGCTGCCTTATTTGTACAAGAAAC


 


CAATAGAACTGACACAAGCCACCAATTGGGGTTGTATATTTTTGGGAGGA


 


AGCGGTAATAACATGGTATATCTTGTTCTGTAATCCTTTTTCTTTAAATT


 


GAATCTCAAGTTAGAGAGCAAAAAAAAAAAAA






The OS34738749 cDNA is translated into the following amino acid sequence (SEQ ID NO:86):








MPSHADLERQIEQLMECKPLSESEVKALCDQARAILVEEWNVQPVKCPVT


 


VCGDIHGQFYDLIELFRIGGNAPDTNYLFMGDYVDRGYYSVETVTLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYL


 


PLTALIESQIFCLHGGLSPSLDTLDNIRALDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIAAQFNHTNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFDPAPRQIEPDTTRK


 


TP-DYFL






cDNA sequence of ZM59400933 from corn (SEQ ID NO:87):








CTGACCGCAGCGGGCCCGCAGGCCGGAGAAGGAGTCGGAGTCGCCCCCAC


 


CCACCCACCCTCTGCCGCGGGCGGGGAGCGGGCGGCGGACGAGATGCCGT


 


CGCACGGGGATCTGGACCGGCAGATCGCGCAGCTGCGCGACTGCAAGTAC


 


CTCGGGCAGGCGGAGGTCAAGGTGCTCTGCGAGCAGGCCAAGGCCATCCT


 


CATGGAGGAATGGAACGTGCAGCCCGTGCGCTGCCCCGTCACCGTCTGCG


 


GCGACATCCACGGCCAGTTCTATGACCTCATCGAGCTCTTCCGCATCGGC


 


GGCGACTCTCCCGACACAACTACCTCTTCATGGGCGACTACGTCGATCGT


 


GGCTATTATTCAGTTGAAACGGTTTCTCTGTTAGTGGCTTTGAAAGTCCG


 


TTACAGAGATAGAATTACAATACTTCGAGGAAATCATGAGAGCAGACAAA


 


TCACTCAAGTGTACGGCTTCTATGATGAATGCTTAAGAAAATATGGAAAT


 


GCAAATGTATGGAAGTATTTTACAGACTTGTTTGATTATTTGCCTCTCAC


 


AGCTCTTATAGAAAATCAGGTCTTCTGTCTTCATGGAGGCCTCTCTCCGT


 


CATTGGACACATTGGATAACATTCGTTCTCTTGATCGCATACAGGAGGTA


 


CCTCATGAAGGACCCATGTGTGATCTTTTGTGGTCTGACCCAGATGACCG


 


ATGTGGGTGGGGAATTTCACCCAGAGGAGCAGGTTACACATTTGGGCAAG


 


ACATTGCACAGCAGTTCAACCATACAAATGGTCTCTCTCTCATTTCAAGG


 


GCCCATCAACTTGTAATGGAAGGATTTAATTGGTGCCAGGATAAGAATGT


 


AGTCACAGTCTTCAGTGCGCCTAATTACTGTTACCGCTGTGGTAACATGG


 


CTGCTATTCTTGAAATCGGGGAAAACATGGACCAGAACTTCCTTCAATTC


 


AACCCCGCACCTCGGCAAATTGAGCCAGACACAACTCGCAAAACCCCAGA


 


CTACTTTCTGTAATTGTGGTGGTGACCTTAACTTTCTGGTGTTTGATGCT


 


CCTCTCTTCCGCAGCATCAGGGTATGTAGATCTTGTCCTTAGATATGGGT


 


CCCATGTGCCCGGCCTTAACGTCTCCCTATTCTTTTGTTTGGAGATTTTG


 


TTTCTGCTTCTCGATCTTGATACAAGATGTTAGAAGTTGAATGCCAGTGT


 


ATTTTTTT-CAAAAAAAAAAA






The ZM59400933 cDNA is translated into the following amino acid sequence (SEQ ID NO:88):








MPSHGDLDRQIAQLRDCKYLPEAEVKVLCEQAKAILMEEWNVQPVRCPVT


 


VCGDIHGQFYDLIELFRIGGDSPDTNYLFMGDYVDRGYYSVETVSLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYL


 


PLTALIENQVFCLHGGLSPSLDTLDNIRSLDRIQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGENMDQNFLQFNPAPRQIEPDTTRK


 


TP-DYFL






cDNA sequence of ZM62132060 from corn (SEQ ID NO:89):








AATCGTCGCTCCACCTCCTCCTCGTCTATCGCCGATCTCCCCCAAACCCT


 


AGCCCCGACCTGACCGCCGGCGGGCCCGCCGGCCGGAGAAGGAGTCGCTC


 


CCACCCATCCAACTTCTGCGGCGGAAGGGGAGCGGGCGGCGGACGAGATG


 


CCGTCGCACGGGGATCTGGACCGGCAGATCGCGCAGCTGCGCGACTGCAA


 


GTACCTGCCCGAGGCGGAGGTCAAGGCGCTCTGCGAGCAGGCCAAGGCCA


 


TCCTTATGGAGGAGTGGAACGTGCAGCCCGTGCGCTGTCCTGTCACCGTC


 


TGTGGCGACATCCACGGCCAGTTCTATGACCTCATCGAGCTCTTCCGCAT


 


CGGCGGCGACGCTCCCGACACCAACTACCTCTTCATGGGCGACTACGTCG


 


ATCGTGGGTACTATTCAGTTGAAACAGTTTCTCTGTTAGTGGCTTTGAAA


 


GTCCGTTACAGAGATAGAATTACAATACTTAGAGGAAATCATGAGAGCAG


 


ACAAATCACTCAAGTATATGGCTTCTATGATGAATGCTTAAGAAAGTATG


 


GAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCT


 


CTCACAGCTCTTATAGAAAATCAGGTCTTCTGTCTTCACGGTGGCCTCTC


 


TCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTACAGG


 


AGGTTCCTCATGAAGGACCCATGTGTGATCTTTTGTGGTCTGACCCAGAT


 


GACCGATGTGGATGGGGAATTTCACCAAGAGGAGCAGGTTACACATTTGG


 


GCAAGACATTGCGCAGCAGTTCAACCATACAAATGGTCTTTCTCTCATTT


 


CAAGGGCCCATCAACTTGTAATGGAAGGATTTAATTGGTGCCAGGATAAG


 


AATGTAGTCACAGTCTTCAGCGCGCCTAATTATTGTTACCGCTGTGGTAA


 


CATGGCTGCTATTCTTGAAATCGGGAAAAACATGGACCAGAACTTCCTTC


 


AATTCGACCCGGCACCTCGGCAAATTGAGCCAGACACAACTCGGAAAACC


 


CCAGACTACTTTTTGTAATTGTGGTGGTGACATTAACTTACTGGTGTTGA


 


TGCTCCTCTTTTCCGCAGCATCAGGGTCTGTAGATCATCTGTCCTTAGAT


 


ATGGGTTCCATGAGCCCGACCTGTACGTCTCCCAATTCTTTTGTTTGGAG


 


ATTTTGTTGCCGCTTAACGATCTTTATACAATATGTTAAAAAGTTAAATG


 


CCATTGGATTTTTCTCCAAAAAAAAAAA






The ZM62132060 cDNA is translated into the following amino acid sequence (SEQ ID NO:90):








MPSHGDLDRQIAQLRDCKYLPEAEVKALCEQAKAILMEEWNVQPVRCPVT


 


VCGDIHGQFYDLIELFRIGGDAPDTNYLFMGDYVDRGYYSVETVSLLVAL


 


KVRYRDRITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDFL


 


PLTALIENQVFCLHGGLSPSLDTLDNIRSLDRVQEVPHEGPMCDLLWSDP


 


DDRCGWGISPRGAGYTFGQDIAQQFNHTNGLSLISRAHQLVMEGFNWCQD


 


KNVVTVFSAPNYCYRCGNMAAILEIGKNMDQNFLQFDPAPRQIEPDTTRK


 


TPDYFL






cDNA sequence of ZM59202533 from corn (SEQ ID NO:91):








ATGAAGGGGAAGAAGCCGGTCAAGGAGCTCAAGCTCACCGTGCCGGCGCA


 


GGAGACCCCGGTAGACAAGTTCCTGACGGCAAGTGGCACGTTCAAGGATG


 


GTGAGCTGAGGCTCAATCAGAGCGGCTTGCGGCTTATCTCTGAGGAAACG


 


GGGGATGAAGATGAATCTACAAAGCTGAAGGTGGAAGATGTGCAGTTATC


 


AATGGATGATCTTGAGATGATTCAAGTCATTGGCAAAGGAAGCGGTGGTG


 


TTGTCCAGCTAGTGAGGCACAAATGGGTGGGCACATTGTTTGCCTTAAAG


 


GGTATTCAAATGAACATTCAGGAGTCAGTTCGTAAACAAATAGTACAGGA


 


GCTCAAAATAAACCAAGCAACACAGAGCCCTCATATAGTTATGTGCCATC


 


AATCTTTTTACCACAATGGTGTAATATATCTTGTTCTTGAGTACATGGAC


 


CGTGGATCGCTTGCAGACATTGTTAAGCAAGTGAAGACTATTCTGGAGCC


 


ATACCTTGCAGTACTTTGTAAGCAGGTCTTGGAGGGTTTATTGTATCTTC


 


ATCATCAAAGGCACGTGATTCACAGGGACATAAAACCATCTAACTTGTTG


 


GTCAACCGTAAAGGTGAAGTCAAGATTACCGACTTCGGAGTGAGTGCTGT


 


GCTAGCAAGCTCAATAGGTCAGCGAGATACATTTGTTGGAACCTACAACT


 


ATATGGCGCCTGAGCGGATTAGTGGTAGCACTTATGACTACAAAAGTGAC


 


ATATGGAGTTTGGGCTTAGTTATACTTGAGTGTGCCATTGGCCGGTTCCC


 


TTATATACCTTCGGAAGGTGAAGGTTGGTTAAGCTTTTATGAACTTCTGG


 


AGGCCATTGTCGATCAGCCACCACCTTCTGCACCTGCAGATCAGTTCTCT


 


CCAGAATTCTGCTCATTTATCTCCTCTTGCATACAGAAAGATCCGGCTCA


 


GAGGATGTCTGCTTCAGAACTCTTGAATCACCCTTTTTTGAAGAAGTTCG


 


AGGATAAGGACTTAAACCTGGGGATTCTTGTGGAGAACCTGGAACCTCCA


 


ATGAATATACCCGAATAG






The ZM59202533 cDNA is translated into the following amino acid sequence (SEQ ID NO:92):








MKGKKPVKELKLTVPAQETPVDKFLTASGTFKDGELRLNQSGLRLISEEN


 


GDEDESTKLKVEDVQLSMDDLEMIQVIGKGSGGVVQLVRHKWVGTLFALK


 


GIQMNIQESVRKQIVQELKINQATQSPHIVMGHQSFYHNGVIYLVLEYMD


 


RGSLADIVKQVKTILEPYLAVLCKQVLEGLLYLHHQRHVIHRDIKPSNLL


 


VNRKGEVKITDFGVSAVLASSIGQRDTFVGTYNYMAPERISGSTYDYKSD


 


IWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFS


 


PEFCSFISSCIQKDPAQRMSASELLNHPFLKKFEDKDLNLGILVENLEPP


 


MNIPE






cDNA sequence of BN41901422 from canola (SEQ ID NO:93):








GTCATTCTTCTAATTTCTCTGACCTCTGCTACTGTCTATCCGTTCGTGTT


 


GCTTTGATCTCTCTAATCAGACATGAAGAGAGGCAGCTTGAGTCTTAATC


 


CCATCTCTCTCCCTCCTCCTGAGCAATCCATCTCCAAATTCTTAACACAG


 


AGCGGAACGTTCAAGGATGGAGACCTTCAAGTGAACAAAGATGGAATCCA


 


GACAGTATCTCATTCTGAGCCTGGAGCTCCACCACCTATTGATCCATTGG


 


ACAACCAGTTGAGTTTGGCTGACCTTGAAGTGATCAAAGTCATTGGCAAA


 


GGAAGCAGTGGTAGTGTTCAGCTGGTTAAACACAAACTAACTCAACAGTT


 


TTTCGCTACTAAGGTTATTCAGTTAAACACAGAAGAGTCCACATGTCGAG


 


CCATTTCTCAGGAGCTGAGGATAAACTTGGCATCTCAATGTCCATATCTC


 


GTCTCATGTTATCAGTCTTTCTACCATAACGGTCTCGTCTCAATCGTAAT


 


GGAGTTCATGGACGGTGGATCTCTTTTGGATTTGTTGAAGAAAGTCCAGA


 


CAGTTCCTGAAAACATGCTCGCTGCCATCTCCAAGCGAGTGCTCCGAGGC


 


TTGTGCTATATTCACGATGAGAGGCGAATCATTCACCGGGACTTGAAGCC


 


TTCCAACTTGCTAATCAATCACAGAGGTGAAGTCAAGATCGCAGACTTTG


 


GTGTCAGCAAGATCTTGTCTAGCACAAGCAGTCTAGCGCATACCTTCGTG


 


GGCACAGACTTCTATATGTCGCCAGAGAGAATCAGTGGGAAAGCGTATGG


 


GAACAAGTGTGATATTTGGAGTTTGGGAGTGGTTCTGCTCGAATGTGCAA


 


CGGGTAAGTTTCCGTATACTCCTCCTGAAAACATGAAGGGATGGACTAGC


 


ATGTATGAGCTAGTTGACGCCATTGTTGAAAACCCGCCTCCTCGTGCACC


 


TTCCCACCTGTTCTCTCCAGAGTTTTGCTCCTTCATCTCGCAATGTGTAC


 


AAAAAGATCCAAGGGACCGGAAATCAGCAATGGAGCTTCTGGACCATAGG


 


TTCGTAAACATGTTTGAAGATGTGGATGTGGATCTCTCGTCTTACTTCAC


 


CGCCGCAGGATCTTTGATTCCCCCACTAGCCAACAGCTAGAACCGAGTTT


 


GAACAATCCTTTTAACACCAAGTTATATATATGTATTTTATATCCACTGG


 


AAGAGACGATATTTACGAGATGTTGCGACTTATGAGAGAATTCTCTTGAT


 


AGACATTTATATTTTCAAGTATTGAAATTTATTTGGGTAAAAAAAAAAAA


 


AAA






The BN41901422 cDNA is translated into the following amino acid sequence (SEQ ID NO:94):








MKRGSLSLNPISLPPPEQSISKFLTQSGTFKDGDLQVNKDGIQTVSHSEP


 


GAPPPIDPLDNQLSLADLEVIKVIGKGSSGSVQLVKHKLTQQFFATKVIQ


 


LNTEESTCRAISQELRINLASQCPYLVSCYQSFYHNGLVSIVMEFMDGGS


 


LLDLLKKVQRVPENMLAAISKRVLRGLCYIHDERRIIHRDLKPSNLLINH


 


RGEVKIADFGVSKILSSTSSLAHTFVGTDFYMSPERISGKAYGNKCDIWS


 


LGVVLLECATGKFPYTPPENMKGWTSMYELVDAIVENPPPRAPSHLFSPE


 


FCSFISQCVQKDPRDRKSAMELLDHRFVNMFEDVDVDLSSYFTAAGSLIP


 


PLANS






cDNA sequence of BN47868329 from canola (SEQ ID NO:95):








CCAGATCGTTAAACCATAATCCAAACCAAGCTTGCAAAAACTTTTGATCC


 


TAAACCGAGATGAAACCAATCCAACCGCCACCAGGAGTAATCGGTCCGGT


 


TAAGAACCGCCCTCGCCGCCGTCCAGACCTCTCCTTACCACTTCCTCACC


 


GCGACGTTTCCCTCGCCGTACCTCTCCCCCTCCCACCAACTTCCGGCGGC


 


GGTTCCACCACCTCAGAGCCTAAAAGCTACTCAGACTTAGTACGTGGCAA


 


CCGGATCGGAAGCGGAGCCGGTGGAACGGTTTACAGAGTAGTCCACCGTC


 


CAACCTCCCGCGTATACGCACTCAAGATAATCAACGGTAACCACGATGAC


 


ACTGTTCGTGGCCAGATCTGCAGAGAGATCAAGATTCTCCGAGACGTGAA


 


TCACCCCAACGTGGTGAAATGCCACGAGATGTTCGATCAAAACGGAGAGA


 


TCCAGGTCTTGCTCGAGCTCATGGACCAAGGATCTTTAGAAGGTGCTCAT


 


ATCTCGAACGAGCAACAGTTATCTGACCTATCTCGTCAGATACTAAACGG


 


TTTGGCTTATCTTCACGGCCGTCATATAGTCCATAGAGACATAAAGCCAT


 


CGAATCTACTTATAAACTCGGACAATAACGTCAAGATTGCTGATTTTGGA


 


GTGAGCAGGGTCTTGGCTCAGACCCTGTCTCCGTGTAAGTCCTCTGTTGG


 


GACTATTGCTTACATGAGTCCTGAGAGGATCAACACGGATTTGAATCAGG


 


GGATGTATGATGGTTGCGCTGGGGATATTTGGAGCTTCGGTGTTAGTGTT


 


CTTGAGTTTTTCTTGGGGAGGTTTCCTTTTAATGTGAATAGGCTAGGTGA


 


TTGGGCTAGTCTTATGTGTGCTATTTGTATGTCTAAGCCGCCTGAAGCTC


 


CTGCCACGGCGTCTCCGGAGTTTAGACACTTTGTTTCGTGTTGTTTGCAG


 


AGAGAACCGGGGAGGAGGCAAACTGCTGTTCAGCTTTTGCAACATCCTTT


 


TGTGCGTAGAGGGGCGATTCAGAGTCAGAATAGGTCTCCTCAGAATCTAC


 


ATCAACTCTTGCCTCCTCCACACTAAAGGTTTAGTTTTGTCTGATAATGT


 


TTCTACACTAAAGGTTGATCATGTCTTGCTGTTTAGACAAACTATATCAT


 


TGTCTTGTACTTAGCTGAAAGCAAAGCGTATATAGTTTGAATCACTTTGC


 


ACCTCATGATGGTTAATTTCACTAAGTAATTCAGTAGTAGAGTCATTAAA


 


TGTAAAAAAAAAAAAAAA






The BN47868329 cDNA is translated into the following amino acid sequence (SEQ ID NO:96):








MKPIQPPPGVIGPVKNRPRRRPDLSLPLPHRDVSLAVPLPLPPTSGGGST


 


TSEPKSYSDLVRGNRIGSGAGGTVYRVVHRPTSRVYALKIINGNHDDTVR


 


GQICREIKILRDVNHPNVVKCHEMFDQNGEIQVLLELMDQGSLEGAHISN


 


EQQLSDLSRQILNGLAYLHGRHIVHRDIKPSNLLINSDNNVKIADFGVSR


 


VLAQTLSPCKSSVGTIAYMSPERINTDLNQGMYDGCAGDIWSFGVSVLEF


 


FLGRFPFNVNRLGDWASLMCAICMSKPPEAPATASPEFRHFVSCCLQREP


 


GRRQTAVQLLQHPFVRRGAIQSQNRSPQNLHQLLPPPH






cDNA sequence of BN42671700 from canola (SEQ ID NO:97):








CTGCAAACTAAAATCTAGAACCGGAACAGATCTAAACCAAACCAAACCGA


 


ACCGGGTGTCTTTGTTTGTAACTCTCCAAATGGTGAAGAAAGCGATGAAG


 


GAGGAAGAAGAAGCAGAGATGAGAAACTCGTCGATGCAGTCAAAGTACAA


 


AGGCGTGAGGAAGAGGAAGTGGGGCAAATGGGTTTCGGAGATCAGACTTC


 


CCAACAGCAGAGAGCGAATCTGGCTAGGCTCTTTCGACACTCCCGAGAAG


 


GCGGCGCGTGCCTTCGACGCCGCCCAGTTTTGTCTCCGCGGCTGCCAATC


 


CGGTTTCAATTTCCCCGATAATCCGCCGTCGATCTCCGGCGGAAGGTCGC


 


TGACGCCTCCGGAGATCCGGGAAGCGGCTGCTCGATACGCAAACGCTCAG


 


GACGACGATATTATCATCACCACCGGAGAAGAAGAATCGGTTTTGTCCGA


 


AACCCGACCGGAGTCTCCTTCAACAACCTCCGTGTCTGAAGCAGATACGT


 


CGCTGGATTGCGATCTATCGTTCTTAGACACGCTTCCTAATGATTTCGGG


 


ATGTTTTCTGTGTTTGATGACTTCTCCGACGGCTTCTCCGGCGATCAGTT


 


TACAGAGGTTTTACCCGTTGAAGATTACGGAGATGTGATTTTTGATGAGT


 


CTCTGTTTCTTTGGGATTTTTAAATGTGTAAAGAGTTTTGAATTGTTGTT


 


TATTCGGGTCATGGAGAGTAATCTGGATATTTTTGTAAGTCGGAGCTCCA


 


GCGACCCGGGAACTTGATCATTCTTGCTTTGGTTGATGATATCTATCATT


 


CCTTCATTTTTTGTTGTTATTAATGAAAATATTTGGATAAAATAGCAATT


 


ACAGAAAAAAAAAAAAAAAAAA






The BN42671700 cDNA is translated into the following amino acid sequence (SEQ ID NO:98):








MVKKAMKEEEEAEMRNSSMQSKYKGVRKRKWGKWVSEIRLPNSRERIWLG


 


SFDTPEKAARAFDAAQFCLRGCQSGFNFPDNPPSISGGRSLTPPEIREAA


 


ARYANAQDDDIIITTGEEESVLSETRPESPSTTSVSEADTSLDGDLSFLD


 


TLPNDFGMESVFDDFSDGFSGDQFTEVLPVEDYGDVIFDESLFLWDF






cDNA sequence of ZM68416988 from corn (SEQ ID NO:99):








CTCGCCTCGCCTTCCTCCGAGCCCCGGCGAGGAAGAGGAACCCGCCGCCG


 


CCGCCGCCGGACGCACTTCCGATGGCGACGCCACGGAAGCCGATCAAGCT


 


CACGCTGCCGTCCCACGAGACCACCATCGGCAAGTTCCTGACGCACAGCG


 


GGACGTTCACGGACGGGGATCTGCGCGTGAACAAGGACGGCCTCCGCATC


 


GTCTCGCGGAGGGAGGGAGGCGAGGCTCCTCCTATAGAGCCGTTGGATAG


 


TCAACTGAGCTTAGATGATCTAGACGTTATAAAAGTGATCGGGAAAGGTA


 


GCAGCGGAAATGTGCAATTGGTCCGCCACAAATTTACTGGCCAGTTTTTT


 


GCTCTGAAGGTTATTCAACTAAATATTGATGAGAGTATACGCAAACAGAT


 


TGCCAAGGAGTTGAAGATAAACTTATCAACACAGTGCCAATATGTTGTTG


 


TGTTCTATCAGTGTTTCTATTTCAATGGTGCCATTTCTATTGTTTTGGAA


 


TACATGGATGGTGGCTCCCTTGCAGATTTCCTGAAGACTGTTAAAACCAT


 


TCCAGAGGCCTACCTCGCTGCTATCTGTACGCAGATGCTAAAAGGACTGA


 


TCTATTTGCATAACGAGAAGCGCGTTATACACCGAGATCTGAAACCATCA


 


AATATATTGATAAATCATAGGGGTGAAGTAAAAATATCAGATTTTGGTGT


 


GAGTGCCATTATATCTAGTTCCTCTTCGCAACGAGATACATTTATTGGCA


 


CACGCAACTACATGGCGCCAGAAAGAATCGATGGAAAGAAACATGGTTCT


 


ATGAGTGATATCTGGAGTTTGGGACTAGTGATACTGGAATGTGCAACCGG


 


CATCTTTCCATTTCCTCCTTGTGAAAGCTTCTACGAACTTCTCGTGGCTG


 


TTGTTGATCAACCGCCACCTTCTGCGCCGCCGGATCAGTTTTCACCAGAA


 


TTCTGTGGGTTCATTTCTGCATGTCTCCAGAAGGATGCTAATGACAGGTC


 


ATCAGCCCAAGCCTTATTGGACCATCCGTTCCTGAGCATGTATGATGACC


 


TGCATGTAGATCTTGCTTCGTACTTCACGACAGCAGGATCTCCTCTCGCC


 


ACCTTCAATTCCAGGCAACTCTAATTTTTTTGTCCTCCTTATTACGCGAA


 


CGGTGTGGCGACAAATTTCTCTTTTTGGACAAGGCTTGGATTGTGTACTG


 


AGCTGTAATGATCTTGTGTGTGTCAGGTCGGTGATTGGCTCCATCACTTT


 


ACATATATGACATACATGTACAGCCTTTTAGGATAAAAATGAGCACTGAA


 


GTTTTGCCTATCTGTATATCGGCAGCAAACGTTTGGTCATGTTTGTTTCA


 


CCTTGTAATGTATTGACTCAGATATGGGATTGGTCATTGTCTCTAAAAAA


 


AAAAA






The ZM68416988 cDNA is translated into the following amino acid sequence (SEQ ID NO:100):








MATPRKPIKLTLPSHETTIGKFLTHSGTFTDGDLRVNKDGLRIVSRREGG


 


EAPPIEPLDSQLSLDDLDVIKVIGKGSSGNVQLVRHKFTGQFFALKVIQL


 


NIDESIRKQIAKELKINLSTQCQYVVVFYQCFYFNGAISIVLEYMDGGSL


 


ADFLKTVKTIPEAYLAAICTQMLKGLIYLHNEKRVIHRDLKPSNILINHR


 


GEVKISDFGVSAIISSSSSQRDTFIGTRNYMAPERIDGKKHGSMSDIWSL


 


GLVILEGATGIFPFPPCESFYELLVAVVDQPPPSAPPDQFSPEFCGFISA


 


CLQKDANDRSSAQALLDHPFLSMYDDLHVDLASYFTTAGSPLATFNSRQL

## Claims

1. A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a full-length polypeptide comprising SEQ ID NO: 16.

2. An isolated polynucleotide comprising of SEQ ID NO: 15.

3. A method of producing a transgenic plant, said method comprising the steps of:
(a) introducing into a plant cell an expression vector comprising a polynucleotide comprising SEQ ID NO: 15, and
(b) generating from the plant cell a transgenic plant that expresses the polynucleotide.