# Patent

## Title
N-substituted piperazines

## Abstract

Novel N-alkyl substituted piperazines have been discovered, which are useful as insecticides or fungicides. Such compounds are of Formula (I)




    
    
        wherein X, Y, R1 and R2 are as defined herein.

## Background
This application claims the benefit of U.S. Provisional Application No. 60/695,364, filed on Jun. 30, 2005, the entire contents of which is hereby incorporated by reference. Additionally, this application claims the benefit of U.S. Non-provisional application Ser. No. 11/479,772, filed 30 Jun. 2006, the entire contents of which is hereby incorporated by reference. The present invention relates to novel N-alkyl substituted piperazines and their use as fungicides and insecticides.


BACKGROUND OF THE INVENTION
Various piperazine derivatives have been disclosed in references such as WO 2004/060865, WO 97/26252, WO 01/46166, JP8-26995, JP63-141966, U.S. 2003/0044845, U.S. Pat. Nos. 6,011,035 and 6,387,897. However, these references do not disclose the piperazine derivatives of the present invention.
Additionally, there continues to be a need for additional insecticidal and fungicidal compounds, due to the continual development of resistance to the insecticides and fungicides currently used in the field. For example, there are at least 400 species of arthropods which are resistant to one or more insecticides. Therefore, the need continues to develop new insecticides and fungicides, and particularly for compounds that have new or atypical modes of action.
SUMMARY OF THE INVENTION
The present invention relates to compounds of Formula (I)




wherein
X and Y are each independently selected from the group consisting of a direct bond, alkyl, carbonyl, carbonylalkyl, carbonylalkylether, carboxylalkyl, alkylcarbonyl, alkylcarboxyl, and alkylether;

    
    
        with the proviso that both X and Y cannot be a direct bond; and when either X or Y is a direct bond, then the R1 or R2 associated with that bond is not phenyl or optionally substituted phenyl; and

R1 and R2 are each independently selected from the group consisting of:

    
    


alkyl;
aryl, optionally substituted with cyano, alkoxy, halogen, alkylhalo, alkoxyhalo, carboxylalkyl, carbonylalkyl, benzyloxy, nitro, benzoyl, phenyl, carboxamido, or heteroaryl;
heteroaryl, optionally substituted with aryl, heteroaryl, alkyl, halogen, alkylhalo, alkoxy, cycloalkyl or cyano;
cycloalkyl, optionally substituted with alkyl or halogen; and
a heterocyclic ring, optionally substituted with alkyl.
The present invention also relates to insecticidal or fungicidal compositions thereof and methods of use.


DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to the compounds of Formula (I), compositions thereof and their use as insecticides and fungicides, in accordance with the definitions herein. In relation to the definitions hereafter, R is an alkyl group; X refers to a halogen atom, and the symbol ‘*’ denotes the point of attachment.

    The term “alkoxy” refers to an alkyl group, attached through an oxygen atom, e.g. *—OR.
    The term “alkoxyhalo” refers to an alkoxy group which is substituted with one or more halogens (*—O—R—Xn).
    The term “alkyl” (including derivative terms such as alkoxy) as used herein includes straight and branched chains containing from 1 to 6 carbon atoms. Thus, typical alkyl groups are methyl, ethyl, 1-methylethyl, propyl, and 1,1-dimethylethyl, and the like.
    The term “alkylcarbonyl” refers to an alkyl group attached to a carbonyl (*—R—C(O)—).
    The term “alkylcarboxyl” refers to an alkyl group attached to a carboxyl group (*—R—C(O)—O—).
    The term “alkylether” refers to an alkyl group attached to an oxygen (*—R—O—).
    The term “alkylhalo” refers to an alkyl group which is substituted with one or more halogens (*—R—Xn).
    The term “aryl” refers to a mono or bicyclic aromatic ring, such as phenyl or naphthalene.
    The term “benzodioxane” refers to a 10 membered, fused, bicyclic aromatic ring containing two oxygen atoms in the para position of the second, 6-membered, non-aromatic ring, i.e. a benzene ring fused to a dioxane ring.
    The term “benzodioxole” refers to a 9 membered, fused, bicyclic aromatic ring containing two non adjacent oxygen atoms in the second, five-membered, non-aromatic ring, i.e. a benzene ring fused to a dioxole ring.
    The term “benzothiophene” refers to a 9 membered, fused, bicyclic aromatic ring containing one sulfur atom, i.e. a benzene ring fused to a thiophene ring.
    The term “benzoyl” refers to a carbonyl group attached to a phenyl group (*—C(O)-phenyl).
    The term “benzyloxy” refers to a benzyl group attached to an oxygen atom (*—O—CH2-phenyl).
    The term “carbonyl” refers to a carbon atom double bonded to an oxygen atom (*—C(O)—).
    The term “carbonylalkyl” refers to a carbonyl group attached to an alkyl group (*—C(O)—R—).
    The term “carbonylalkylether” refers to a carbonyl group attached to an alkylether group (*—C(O)—R—O—).
    The term “carboxamido” refers to a carbonyl group attached to an amino group (*—C(O)—NH2).
    The term “carboxylalkyl” refers to a carboxyl group attached to an alkyl group (*—C(O)—O—R—).
    The term “cyano” refers to a carbon atom triple bonded to a nitrogen atom (*—C≡N).
    The term “cycloalkyl” refers to a saturated C3-C6 membered ring, such as cyclobutane, cyclopropane, cyclohexane and the like.
    The term “halogen” or “halo” refers to the atoms fluorine, chlorine, bromine, and iodine.
    The term “heteroaryl” refers to a 5 or 6 membered unsaturated ring containing one, two or three heteroatoms, preferably one or two heteroatoms independently selected from oxygen, nitrogen and sulfur; or to a bicyclic unsaturated ring system containing up to 11 atoms including one, two or three heteroatoms selected from oxygen, nitrogen and sulfur. Examples of heteroaryl ringss include, but are not limited to, 2-, 3- or 4-pyridine, pyrazine, pyrazole, 2-, 4-, or 5-pyrimidine, 2- or 3-thiophene, 2- or 3-furan, oxazole, quinoline, benzofuran, benzopyronye, benzothiophene, thiophenepyrazoline, thiadiazole, benzodioxane, benzodioxole, indole, pyridazine, triazole, imidazole, pyrrole, isoxazole, thiazole, isothiazole, oxadiazole, thiadiazole isoquinoline and the like. The ring may be attached at any position so long as the laws of chemical valency and stereochemistry are observed.
    The term “heterocyclic” refers to a 3 to 5 membered, saturated ring containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur; such as oxirane, pyrrolidine, morpholine rings and the like. The ring may be attached at any position so long as the laws of chemical valency and stereochemistry are observed.
    The term “nitro” refers to a nitrogen atom bonded to two oxygen atoms, (*—NO2).
    The term “oxazole” refers to a 5 membered, aromatic ring containing one oxygen atom and one nitrogen atom, non-adjacent to each other, i.e. an oxazole ring.
    The term “oxirane” refers to a 3 membered ring containing oxygen and carbon, i.e.






    The term “pyrazine” refers to a 6 membered, aromatic ring containing 2 nitrogen atoms, wherein the N's are in the para position.
    The term “pyrazole” refers to a 5 membered, aromatic ring containing 2 nitrogen atoms, wherein the N's are adjacent to one another.
    The term “thiadiazole” refers to a 5 membered, aromatic ring containing two nitrogen atoms and a sulfur atom.
    The term “thiophenepyrazoline” refers to a 8 membered, fused, bicyclic aromatic ring containing one sulfur atom, and two nitrogen atoms, wherein the sulfur atom is part of a thiophene ring and the nitrogen atoms are part of a pyrazoline ring fused therewith.


In one embodiment of the present invention X and Y are each independently selected from the group consisting of a direct bond, alkyl, and carbonyl;

    
    
        with the proviso that both X and Y cannot be a direct bond; and when either X or Y is a direct bond, then the R1 or R2 associated with that bond is not phenyl or optionally substituted phenyl; and

R1 and R2 are each independently selected from the group consisting of:

    
    


aryl, optionally substituted with cyano, alkoxy, halogen, alkylhalo, alkoxyhalo, carboxylalkyl, carbonylalkyl, benzyloxy, nitro, benzoyl, phenyl, carboxamido, or heteroaryl; and
heteroaryl, optionally substituted with aryl, heteroaryl, alkyl, halogen, alkylhalo, alkoxy, cycloalkyl or cyano.
The compounds of the present invention can exist as one or more stereoisomers. The various stereoisomers include geometric isomers, diastereomers and enantiomers. Thus the compounds of the present invention include racemic mixtures, individual stereoisomers and optically active mixtures. It will be appreciated by those skilled in the art that one stereoisomer may be more active than the others. Individual stereoisomers and optically active mixtures may be obtained by selective synthetic procedures, by conventional synthetic procedures using resolved starting materials or by conventional resolution procedures.
The compounds of the present invention can be prepared by known methods in the art as outlined in the examples of the present specification.
Another aspect of the present invention relates to an insecticidal or fungicidal composition comprising a compound of Formula (I) and a phytologically-acceptable inert carrier. The compositions are typically either concentrated formulations which are dispersed in water for application, or are dust or granular formulations which are applied without further treatment. The compositions are prepared according to procedures and formulae which are conventional in the agricultural chemical art, but which are novel and important because of the presence therein of the compounds of this invention. Some description of the formulation of the compositions will be given, however, to assure that agricultural chemists can readily prepare any desired composition.
The dispersions in which the compounds are applied are most often aqueous suspensions or emulsions prepared from concentrated formulations of the compounds. Such water-soluble, water-suspendable or emulsifiable formulations are either solids, usually known as wettable powders, or liquids usually known as emulsifiable concentrates or aqueous suspensions. Wettable powders, which may be compacted to form water dispersible granules, comprise an intimate mixture of the active compound, an inert carrier, and surfactants. The concentration of the active compound is usually from about 10% to about 90% by weight. The inert carrier is usually chosen from among the attapulgite clays, the montmorillonite clays, the diatomaceous earths, or the purified silicates. Effective surfactants, comprising from about 0.5% to about 10% of the wettable powder, are found among the sulfonated lignins, the condensed naphthalenesulfonates, the naphthalenesulfonates, the alkylbenzenesulfonates, the alkyl sulfates, and nonionic surfactants such as ethylene oxide adducts of alkyl phenols.
Emulsifiable concentrates of the compounds comprise a convenient concentration of a compound, such as from about 50 to about 500 grams per liter of liquid, equivalent to about 10% to about 50%, dissolved in an inert carrier which is either a water miscible solvent or a mixture of water-immiscible organic solvent and emulsifiers. Useful organic solvents include aromatics, especially the xylenes, and the petroleum fractions, especially the high-boiling naphthalenic and olefinic portions of petroleum such as heavy aromatic naphtha. Other organic solvents may also be used, such as the terpenic solvents including rosin derivatives, aliphatic ketones such as cyclohexanone, and complex alcohols such as 2-ethoxyethanol. Suitable emulsifiers for emulsifiable concentrates are chosen from conventional nonionic surfactants, such as those discussed above.
Aqueous suspensions comprise suspensions of water-insoluble compounds of this invention, dispersed in an aqueous vehicle at a concentration in the range from about 5% to about 50% by weight. Suspensions are prepared by finely grinding the compound, and vigorously mixing it into a vehicle comprised of water and surfactants chosen from the same types discussed above. Inert ingredients, such as inorganic salts and synthetic or natural gums, may also be added, to increase the density and viscosity of the aqueous vehicle. It is often most effective to grind and mix the compound at the same time by preparing the aqueous mixture, and homogenizing it in an implement such as a sand mill, ball mill, or piston-type homogenizer.
The compounds may also be applied as granular compositions, which are particularly useful for applications to the soil. Granular compositions usually contain from about 0.5% to about 10% by weight of the compound, dispersed in an inert carrier which consists entirely or in large part of clay or a similar inexpensive substance. Such compositions are usually prepared by dissolving the compound in a suitable solvent and applying it to a granular carrier which has been pre-formed to the appropriate particle size, in the range of from about 0.5 to 3 mm. Such compositions may also be formulated by making a dough or paste of the carrier and compound and crushing and drying to obtain the desired granular particle size.
Dusts containing the compounds are prepared simply by intimately mixing the compound in powdered form with a suitable dusty agricultural carrier, such as kaolin clay, ground volcanic rock, and the like. Dusts can suitably contain from about 1% to about 10% of the compound.
It is equally practical, when desirable for any reason, to apply the compound in the form of a solution in an appropriate organic solvent, usually a bland petroleum oil, such as the spray oils, which are widely used in agricultural chemistry.
Insecticides, acaricides and fungicides are generally applied in the form of a dispersion of the active ingredient in a liquid carrier. It is conventional to refer to application rates in terms of the concentration of active ingredient in the carrier. The most widely used carrier is water.
The compounds of the present invention can also be applied in the form of an aerosol composition. In such compositions the active compound is dissolved or dispersed in an inert carrier, which is a pressure-generating propellant mixture. The aerosol composition is packaged in a container from which the mixture is dispensed through an atomizing valve. Propellant mixtures comprise either low-boiling halocarbons, which may be mixed with organic solvents, or aqueous suspensions pressurized with inert gases or gaseous hydrocarbons.
The formulations may additionally contain adjuvant surfactants to enhance deposition, wetting and penetration of the compounds onto the target crop and organism. These adjuvant surfactants may optionally be employed as a component of the formulation or as a tank mix. The amount of adjuvant surfactant will typically vary from 0.01 to 1.0% by volume, based on a spray-volume of water, preferably 0.05 to 0.5 volume %. Suitable adjuvant surfactants include, but are not limited to ethoxylated nonyl phenols, ethoxylated synthetic or natural alcohols, salts of the esters or sulphosuccinic acids, ethoxylated organosilicones, ethoxylated fatty amines and blends of surfactants with mineral or vegetable oils.
Another aspect of the present invention relates to a method for inhibiting an insect which comprises applying to a locus of the insect an insect-inhibiting amount of a compound of Formula (I).
The “locus” of insects is a term used herein to refer to the environment in which the insects live or where their eggs are present, including the air surrounding them, the food they eat, or objects which they contact. For example, insects which eat or contact edible or ornamental plants can be controlled by applying the active compound to plant parts such as the seed, seedling, or cutting which is planted, the leaves, stems, fruits, grain, or roots, or to the soil in which the roots are growing. It is contemplated that the compounds might also be useful to protect textiles, paper, stored grain, seeds, domesticated animals, buildings or human beings by applying an active compound to or near such objects. The term “inhibiting an insect” refers to a decrease in the numbers of living insects, or a decrease in the number of viable insect eggs. The extent of reduction accomplished by a compound depends, of course, upon the application rate of the compound, the particular compound used, and the target insect species. At least an inactivating amount should be used. The terms “insect-inactivating amount” are used to describe the amount, which is sufficient to cause a measurable reduction in the treated insect population. Generally an amount in the range from about 1 to about 1000 ppm by weight active compound is used.
The actual amount of compound to be applied to loci of insects and mites is not critical and can readily be determined by those skilled in the art in view of the examples above. In general, concentrations from 10 ppm to 5000 ppm by weight of compound are expected to provide good control. With many of the compounds, concentrations from 100 to 1500 ppm will suffice.
The locus to which a compound is applied can be any locus inhabited by an insect or mite, for example, vegetable crops, fruit and nut trees, grape vines, ornamental plants, domesticated animals, the interior or exterior surfaces of buildings, and the soil around buildings.
Another embodiment of the present invention is a method for the control or prevention of fungal attack. This method comprises applying to the soil, plant, roots, foliage, seed or locus of the fungus, or to a locus in which the infestation is to be prevented (for example applying to cereal or grape plants), a fungicidal effective amount of one or more of the compounds. The compounds are suitable for treatment of various plants at fungicidal levels, while exhibiting low phytotoxicity. The compounds are useful both in a protectant and/or an eradicant fashion.
The compounds have been found to have significant fungicidal effect particularly for agricultural use. Many of the compounds are particularly effective for use with agricultural crops and horticultural plants. It will be understood by those in the art that the efficacy of the compound for the foregoing fungi establishes the general utility of the compounds as fungicides.
The compounds have broad ranges of efficacy as fungicides. The exact amount of the active material to be applied is dependent not only on the specific active material being applied, but also on the particular action desired, the fungal species to be controlled, and the stage of growth thereof, as well as the part of the plant or other product to be contacted with the compound. Thus, all the compounds, and formulations containing the same, may not be equally effective at similar concentrations or against the same fungal species.
The compounds are effective in use with plants in a disease-inhibiting and phytologically acceptable amount. The term “disease inhibiting and phytologically acceptable amount” refers to an amount of a compound that kills or inhibits the plant disease for which control is desired, but is not significantly toxic to the plant. This amount will generally be from about 0.1 to about 1000 ppm (parts per million), with 1 to 500 ppm being preferred. The exact concentration of compound required varies with the fungal disease to be controlled, the type of formulation employed, the method of application, the particular plant species, climate conditions, and the like. A suitable application rate is typically in the range from about 0.10 to about 4 pounds/acre (about 0.01 to 0.45 grams per square meter, g/m2).
Additionally, the compounds of the present invention (Formula I) are often applied in conjunction with one or more other insecticides or fungicides to obtain control of a wider variety of pests and diseases. Such additional pesticidal compounds may be fungicides, insecticides, nematocides, miticides, arthropodicides, bactericides or combinations thereof that are compatible with the compounds of the present invention in the medium selected for application, and not antagonistic to the activity of the present compounds. Accordingly, in such embodiments, the other pesticidal compound is employed as a supplemental toxicant for the same or for a different pesticidal use. The compounds of Formula I, and the pesticidal compound in the combination can generally be present in a weight ratio of from 1:100 to 100:1.
When used in conjunction with other insecticides or fungicides, the presently claimed compounds can be formulated with the other insecticides or fungicides, tank mixed with the other insecticides or fungicides, or applied sequentially with the other insecticides or fungicides.
Some of the insecticides that can be employed beneficially in combination with the compounds of the present invention include, but are not limited to: antibiotic insecticides such as allosamidin and thuringiensin; macrocyclic lactone insecticides such as spinosad; avermectin insecticides such as abamectin, doramectin, emamectin, eprinomectin, ivermectin and selamectin; milbemycin insecticides such as lepimectin, milbemectin, milbemycin oxime and moxidectin; arsenical insecticides such as calcium arsenate, copper acetoarsenite, copper arsenate, lead arsenate, potassium arsenite and sodium arsenite; botanical insecticides such as anabasine, azadirachtin, d-limonene, nicotine, pyrethrins, cinerins, cinerin I, cinerin II, jasmolin I, jasmolin II, pyrethrin I, pyrethrin II, quassia, rotenone, ryania and sabadilla; carbamate insecticides such as bendiocarb and carbaryl; benzofuranyl methylcarbamate insecticides such as benfuracarb, carbofuran, carbosulfan, decarbofuran and furathiocarb; dimethylcarbamate insecticides dimitan, dimetilan, hyquincarb and pirimicarb; oxime carbamate insecticides such as alanycarb, aldicarb, aldoxycarb, butocarboxim, butoxycarboxim, methomyl, nitrilacarb, oxamyl, tazimcarb, thiocarboxime, thiodicarb and thiofanox; phenyl methylcarbamate insecticides such as allyxycarb, aminocarb, bufencarb, butacarb, carbanolate, cloethocarb, dicresyl, dioxacarb, EMPC, ethiofencarb, fenethacarb, fenobucarb, isoprocarb, methiocarb, metolcarb, mexacarbate, promacyl, promecarb, propoxur, trimethacarb, XMC and xylylcarb; dinitrophenol insecticides such as dinex, dinoprop, dinosam and DNOC; fluorine insecticides such as barium hexafluorosilicate, cryolite, sodium fluoride, sodium hexafluorosilicate and sulfluramid; formamidine insecticides such as amitraz, chlordimeform, formetanate and formparanate; fumigant insecticides such as acrylonitrile, carbon disulfide, carbon tetrachloride, chloroform, chloropicrin, para-dichlorobenzene, 1,2-dichloropropane, ethyl formate, ethylene dibromide, ethylene dichloride, ethylene oxide, hydrogen cyanide, iodomethane, methyl bromide, methylchloroform, methylene chloride, naphthalene, phosphine, sulfuryl fluoride and tetrachloroethane; inorganic insecticides such as borax, calcium polysulfide, copper oleate, mercurous chloride, potassium thiocyanate and sodium thiocyanate; chitin synthesis inhibitors such as bistrifluoron, buprofezin, chlorfluazuron, cyromazine, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, noviflumuron, penfluoron, teflubenzuron and triflumuron; juvenile hormone mimics such as epofenonane, fenoxycarb, hydroprene, kinoprene, methoprene, pyriproxyfen and triprene; juvenile hormones such as juvenile hormone I, juvenile hormone II and juvenile hormone III; moulting hormone agonists such as chromafenozide, halofenozide, methoxyfenozide and tebufenozide; moulting hormones such as α-ecdysone and ecdysterone; moulting inhibitors such as diofenolan; precocenes such as precocene I, precocene II and precocene III; unclassified insect growth regulators such as dicyclanil; nereistoxin analogue insecticides such as bensultap, cartap, thiocyclam and thiosultap; nicotinoid insecticides such as flonicamid; nitroguanidine insecticides such as clothianidin, dinotefuran, imidacloprid and thiamethoxam; nitromethylene insecticides such as nitenpyram and nithiazine; pyridylmethylamine insecticides such as acetamiprid, imidacloprid, nitenpyram and thiacloprid; organochlorine insecticides such as bromo-DDT, camphechlor, DDT, pp′-DDT, ethyl-DDD, HCH, gamma-HCH, lindane, methoxychlor, pentachlorophenol and TDE; cyclodiene insecticides such as aldrin, bromocyclen, chlorbicyclen, chlordane, chlordecone, dieldrin, dilor, endosulfan, endrin, HEOD, heptachlor, HHDN, isobenzan, isodrin, kelevan and mirex; organophosphate insecticides such as bromfenvinfos, chlorfenvinphos, crotoxyphos, dichlorvos, dicrotophos, dimethylvinphos, fospirate, heptenophos, methocrotophos, mevinphos, monocrotophos, naled, naftalofos, phosphamidon, propaphos, TEPP and tetrachlorvinphos; organothiophosphate insecticides such as dioxabenzofos, fosmethilan and phenthoate; aliphatic organothiophosphate insecticides such as acethion, amiton, cadusafos, chlorethoxyfos, chlormephos, demephion, demephion-O, demephion-S, demeton, demeton-O, demeton-S, demeton-methyl, demeton-O-methyl, demeton-S-methyl, demeton-S-methylsulphon, disulfoton, ethion, ethoprophos, IPSP, isothioate, malathion, methacrifos, oxydemeton-methyl, oxydeprofos, oxydisulfoton, phorate, sulfotep, terbufos and thiometon; aliphatic amide organothiophosphate insecticides such as amidithion, cyanthoate, dimethoate, ethoate-methyl, formothion, mecarbam, omethoate, prothoate, sophamide and vamidothion; oxime organothiophosphate insecticides such as chlorphoxim, phoxim and phoxim-methyl; heterocyclic organothiophosphate insecticides such as azamethiphos, coumaphos, coumithoate, dioxathion, endothion, menazon, morphothion, phosalone, pyraclofos, pyridaphenthion and quinothion; benzothiopyran organothiophosphate insecticides such as dithicrofos and thicrofos; benzotriazine organothiophosphate insecticides such as azinphos-ethyl and azinphos-methyl; isoindole organothiophosphate insecticides such as dialifos and phosmet; isoxazole organothiophosphate insecticides such as isoxathion and zolaprofos; pyrazolopyrimidine organothiophosphate insecticides such as chlorprazophos and pyrazophos; pyridine organothiophosphate insecticides such as chlorpyrifos and chlorpyrifos-methyl; pyrimidine organothiophosphate insecticides such as butathiofos, diazinon, etrimfos, lirimfos, pirimiphos-ethyl, pirimiphos-methyl, primidophos, pyrimitate and tebupirimfos; quinoxaline organothiophosphate insecticides such as quinalphos and quinalphos-methyl; thiadiazole organothiophosphate insecticides such as athidathion, lythidathion, methidathion and prothidathion; triazole organothiophosphate insecticides such as isazofos and triazophos; phenyl organothiophosphate insecticides such as azothoate, bromophos, bromophos-ethyl, carbophenothion, chlorthiophos, cyanophos, cythioate, dicapthon, dichlofenthion, etaphos, famphur, fenchlorphos, fenitrothion fensulfothion, fenthion, fenthion-ethyl, heterophos, jodfenphos, mesulfenfos, parathion, parathion-methyl, phenkapton, phosnichlor, profenofos, prothiofos, sulprofos, temephos, trichlormetaphos-3 and trifenofos; phosphonate insecticides such as butonate and trichlorfon; phosphonothioate insecticides such as mecarphon; phenyl ethylphosphonothioate insecticides such as fonofos and trichloronat; phenyl phenylphosphonothioate insecticides such as cyanofenphos, EPN and leptophos; phosphoramidate insecticides such as crufomate, fenamiphos, fosthietan, mephosfolan, phosfolan and pirimetaphos; phosphoramidothioate insecticides such as acephate, isocarbophos, isofenphos, methamidophos and propetamphos; phosphorodiamide insecticides such as dimefox, mazidox, mipafox and schradan; oxadiazine insecticides such as indoxacarb; phthalimide insecticides such as dialifos, phosmet and tetramethrin; pyrazole insecticides such as acetoprole, ethiprole, fipronil, pyrafluprole, pyriprole, tebufenpyrad, tolfenpyrad and vaniliprole; pyrethroid ester insecticides such as acrinathrin, allethrin, bioallethrin, barthrin, bifenthrin, bioethanomethrin, cyclethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin, zeta-cypermethrin, cyphenothrin, deltamethrin, dimefluthrin, dimethrin, empenthrin, fenfluthrin, fenpirithrin, fenpropathrin, fenvalerate, esfenvalerate, flucythrinate, fluvalinate, tau-fluvalinate, furethrin, imiprothrin, metofluthrin, permethrin, biopermethrin, transpermethrin, phenothrin, prallethrin, profluthrin, pyresmethrin, resmethrin, bioresmethrin, cismethrin, tefluthrin, terallethrin, tetramethrin, tralomethrin and transfluthrin; pyrethroid ether insecticides such as etofenprox, flufenprox, halfenprox, protrifenbute and silafluofen; pyrimidinamine insecticides such as flufenerim and pyrimidifen; pyrrole insecticides such as chlorfenapyr; tetronic acid insecticides such as spiromesifen; thiourea insecticides such as diafenthiuron; urea insecticides such as flucofuron and sulcofuron; and unclassified insecticides such as closantel, crotamiton, EXD, fenazaflor, fenoxacrim, flubendiamide, hydramethylnon, isoprothiolane, malonoben, metaflumizone, metoxadiazone, nifluridide, pyridaben, pyridalyl, rafoxanide, triarathene and triazamate and any combinations thereof.
Some of the fungicides that can be employed beneficially in combination with the compounds of the present invention include, but are not limited to: enestrobin, antimycin, quinoxyfen, SYP-048, IK-1140, NC-224, 2-(thiocyanatomethylthio)-benzothiazole, 2-phenylphenol, 8-hydroxyquinoline sulfate, Ampelomyces, quisqualis, azaconazole, azoxystrobin, Bacillus subtilis, benalaxyl, benomyl, benthiavalicarb-isopropyl, benzylaminobenzene-sulfonate (BABS) salt, bicarbonates, biphenyl, bismerthiazol, bitertanol, blasticidin-S, borax, Bordeaux mixture, boscalid, bromuconazole, bupirimate, calcium polysulfide, captafol, captan, carbendazim, carboxin, carpropamid, carvone, chloroneb, chlorothalonil, chlozolinate, Coniothyrium minitans, copper hydroxide, copper octanoate, copper oxychloride, copper sulfate, copper sulfate (tribasic), cuprous oxide, cyazofamid, cyflufenamid, cymoxanil, cyproconazole, cyprodinil, dazomet, debacarb, diammonium ethylenebis-(dithiocarbamate), dichlofluanid, dichlorophen, diclocymet, diclomezine, dichloran, diethofencarb, difenoconazole, difenzoquat ion, diflumetorim, dimethomorph, dimoxystrobin, diniconazole, diniconazole-M, dinobuton, dinocap, diphenylamine, dithianon, dodemorph, dodemorph acetate, dodine, dodine free base, edifenphos, epoxiconazole, ethaboxam, ethoxyquin, etridiazole, famoxadone, fenamidone, fenarimol, fenbuconazole, fenfuram, fenhexamid, fenoxanil, fenpiclonil, fenpropidin, fenpropimorph, fentin, fentin acetate, fentin hydroxide, ferbam, ferimzone, fluazinam, fludioxonil, flumorph, fluopicolide, fluoroimide, fluoxastrobin, fluquinconazole, flusilazole, flusulfamide, flutolanil, flutriafol, folpet, formaldehyde, fosetyl, fosetyl-aluminium, fuberidazole, furalaxyl, furametpyr, guazatine, guazatine acetates, GY-81, hexachlorobenzene, hexaconazole, hymexazol, imazalil, imazalil sulfate, imibenconazole, iminoctadine, iminoctadine triacetate, iminoctadine tris(albesilate), ipconazole, iprobenfos, iprodione, iprovalicarb, isoprothiolane, kasugamycin, kasugamycin hydrochloride hydrate, kresoxim-methyl, mancopper, mancozeb, maneb, mepanipyrim, mepronil, mercuric chloride, mercuric oxide, mercurous chloride, metalaxyl, mefenoxam, metalaxyl-M, metam, metam-ammonium, metam-potassium, metam-sodium, metconazole, methasulfocarb, methyl iodide, methyl isothiocyanate, metiram, metominostrobin, metrafenone, mildiomycin, myclobutanil, nabam, nitrothal-isopropyl, nuarimol, octhilinone, ofurace, oleic acid (fatty acids), orysastrobin, oxadixyl, oxine-copper, oxpoconazole fumarate, oxycarboxin, pefurazoate, penconazole, pencycuron, pentachlorophenol, pentachlorophenyl laurate, penthiopyrad, phenylmercury acetate, phosphonic acid, phthalide, picoxystrobin, polyoxin B, polyoxins, polyoxorim, potassium bicarbonate, potassium hydroxyquinoline sulfate, probenazole, prochloraz, procymidone, propamocarb, propamocarb hydrochloride, propiconazole, propineb, proquinazid, prothioconazole, pyraclostrobin, pyrazophos, pyributicarb, pyrifenox, pyrimethanil, pyroquilon, quinoclamine, quinoxyfen, quintozene, Reynoutria sachalinensis extract, silthiofam, simeconazole, sodium 2-phenylphenoxide, sodium bicarbonate, sodium pentachlorophenoxide, spiroxamine, sulfur, SYP-Z071, tar oils, tebuconazole, tecnazene, tetraconazole, thiabendazole, thifluzamide, thiophanate-methyl, thiram, tiadinil, tolclofos-methyl, tolylfluanid, triadimefon, triadimenol, triazoxide, tricyclazole, tridemorph, trifloxystrobin, triflumizole, triforine, triticonazole, validamycin, vinclozolin, zineb, ziram, zoxamide, Candida oleophila, Fusarium oxysporum, Gliocladium spp., Phlebiopsis gigantean, Streptomyces griseoviridis, Trichoderma spp., (RS)—N-(3,5-dichlorophenyl)-2-(methoxymethyl)-succinimide, 1,2-dichloropropane, 1,3-dichloro-1,1,3,3-tetrafluoroacetone hydrate, 1-chloro-2,4-dinitronaphthalene, 1-chloro-2-nitropropane, 2-(2-heptadecyl-2-imidazolin-1-yl)ethanol, 2,3-dihydro-5-phenyl-1,4-dithi-ine 1,1,4,4-tetraoxide, 2-methoxyethylmercury acetate, 2-methoxyethylmercury chloride, 2-methoxyethylmercury silicate, 3-(4-chlorophenyl)-5-methylrhodanine, 4-(2-nitroprop-1-enyl)phenyl thiocyanateme: ampropylfos, anilazine, azithiram, barium polysulfide, Bayer 32394, benodanil, benquinox, bentaluron, benzamacril; benzamacril-isobutyl, benzamorf, binapacryl, bis(methylmercury) sulfate, bis(tributyltin) oxide, buthiobate, cadmium calcium copper zinc chromate sulfate, carbamorph, CECA, chlobenthiazone, chloraniformethan, chlorfenazole, chlorquinox, climbazole, copper bis(3-phenylsalicylate), copper zinc chromate, cufraneb, cupric hydrazinium sulfate, cuprobam, cyclafuramid, cypendazole, cyprofuram, decafentin, dichlone, dichlozoline, diclobutrazol, dimethirimol, dinocton, dinosulfon, dinoterbon, dipyrithione, ditalimfos, dodicin, drazoxolon, EBP, ESBP, etaconazole, etem, ethirim, fenaminosulf, fenapanil, fenitropan, fluotrimazole, furcarbanil, furconazole, furconazole-cis, furmecyclox, furophanate, glyodine, griseofulvin, halacrinate, Hercules 3944, hexylthiofos, ICIA0858, isopamphos, isovaledione, mebenil, mecarbinzid, metazoxolon, methfuroxam, methylmercury dicyandiamide, metsulfovax, milneb, mucochloric anhydride, myclozolin, N-3,5-dichlorophenyl-succinimide, N-3-nitrophenyl-itaconimide, natamycin, N-ethylmercurio-4-toluenesulfonanilide, nickel bis(dimethyldithiocarbamate), OCH, phenylmercury dimethyldithiocarbamate, phenylmercury nitrate, phosdiphen, prothiocarb; prothiocarb hydrochloride, pyracarbolid, pyridinitril, pyroxychlor, pyroxyfur, quinacetol; quinacetol sulfate, quinazamid, quinconazole, rabenzazole, salicylanilide, SSF-109, sultropen, tecoram, thiadifluor, thicyofen, thiochlorfenphim, thiophanate, thioquinox, tioxymid, triamiphos, triarimol, triazbutil, trichlamide, urbacid, XRD-563, and zarilamid, and any combinations thereof.
EXAMPLES
Throughout the present specification, all temperatures are given in degrees Celsius, and all percentages are weight percentages unless otherwise stated. The following examples are representative of the embodiments of the present invention and should not be read as limiting.
The compounds of the present invention may be synthesized by methods that are individually known to those skilled in the art.
The compounds of Formula I wherein R1, R2, X, Y, are previously defined can be prepared by the methods illustrated in the following schemes.




In step a, of scheme A, alcohol 1 is treated with methan sulfonyl chloride and triethylamine (Et3N) at 0° C. to yield mesylate 2, which undergoes a nucleophilic displacement with piperazine in step b, to deliver compound 4. The preferred solvent for the nucleophilic displacement is acetonitrile (CH3CN) and elevated temperatures are required to drive the reaction to completion. Commercial mono-substituted piperazines were used directly in schemes B and C when available.




As depicted in step a, a substituted piperazine, 4, is reacted with an acid chloride 5 to yield the appropriately acyl piperazine 6. The acylation is performed under basic conditions to neutralize the hydrochloric acid (HCl) formed during the reaction; examples of typical bases are diispropylamine (DIEA) and Et3N.




As depicted in Scheme C, a substituted piperazine, 4, is reacted with chloro derivative 7 (note bromides can also be used) and heated at 80° C. in dimethylformamide (DMF) to give the appropriately substituted piperazine 8. The reaction is performed under basic conditions to neutralize the HCl formed during the reaction; examples of typical bases are DIEA and Et3N. Alternatively, t-butylcarbamate (BOC) protected piperazine can be utilized as illustrated in schemes D and E. In step a, of scheme D, BOC protected piperazine is N-acylated with an acid chloride utilizing similar reactions previously described to yield compound 10. The BOC group is removed with trifluoroacetic acid (TFA) in methylene chloride (CH2Cl2) and the free base 11, is N-alkylated, again utilizing reaction conditions described earlier, to yield derivatives of 6.




The N-acylation and N-alkylation steps in scheme D can be reversed as shown in scheme E to deliver derivatives of 6.




The following examples further illustrate the present invention, but, are not to be construed as in any way limiting its scope.
Example I
1-Benzyl-4-[(3,5-dimethylisoxazol-4-yl)carbonyl]piperazine (14)




To a 2-dram screw-cap vial was added polymer-supported diisopropylethylamine (PS-DIEA, 54 mg, 0.2 mmol based on 3.68 mmol/g loading of resin) and DMF (2 mL). The vials were placed in an orbital shaker allowed to shake for 15 min. 1-Benzylpiperazine (35 mg, 0.2 mmol) was dissolved in DMF (100 μL) and transferred by pipette to the vial. 3,5-Dimethylisoxazole-4-carbonyl chloride (32 mg, 0.2 mmol), was dissolve in DMF (100 μL) and transferred to the vial. The vial was capped and allowed to shake at room temperature (RT) for 12 h. The reaction mixture filtered and solvent evaporated to give the desired acylated piperazine. MS ELS+ 300.
Example II
1-(2-methyoxybenzoyl)-4-{2-[2-trifluoromethyl)phenyl]ethyl}piperazine (15)




1-[2-(Trifluoromethyl)phenethyl]piperazine (200 mg, 0.60 mmol), o-anisyl chloride (100 mg, 0.59 mmol), and Et3N (253 μL, 1.81 mmol) were dissolved in DMF (10 mL) and stirred at ambient temperature for 12 h. The reaction mixture was extracted with diethylether (Et2O) and the combined organic layers washed with brine, dried over anhydrous sodium sulfate (Na2SO4) and evaporated to give an amber oil (120 mg, 51%). 1H NMR (CDCl3) δ: 2.4-2.7 (m, 6H), 2.9-3.0 (t, 2H), 3.2-3.35 (m, 2H), 3.8-3.95 (m, 5H), 6.89-7.01 (m, 2H), 7.22-7.37 (m, 4H), 7.43-7.49 (t, 1H), 7.59-7.62 (d, 1H). MS ELS+ 393.
Example III
1-[(6-chloro-1,3-benzodioxol-5-yl)methyl]-4-(2-phenoxyethyl)piperazine (16)




1-[2-(phenoxy)-ethyl]piperazine (200 mg, 0.974 mmol), 6-chloropiperonyl chloride (200 mg, 0.974 mmol), and Et3N (415 μL, 3 mmol) were combined in tetrahydrofuran (THF 20 mL). The reaction mixture was heated at 60° C. for 12 h. The reaction mixture was cooled and extracted with ethyl acetate (EtOAc). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and evaporated. The crude residue was chromatographed on silica (20% EtOAc/Hexanes) and the product fractions were collected and evaporated to an amber solid (186 mg, 51%). 1H NMR (CDCl3) δ: 2.5-2.7 (m, 8H), 2.8 (t, 2H), 3.5 (s, 2H), 4.1 (t, 2H), 5.9 (s, 2H), 6.81 (s, 1H), 6.9-6.94 (m, 3H), 6.96-6.98 (m, 1H), 7.26-7.3 (m, 1H). MS ELS+ 376. CHN: calc., 64.08, 6.18, 7.47; found, 63.87, 6.12, 7.44. mp: 72-76° C.
Example IV
3-{[4-(2-phenylethyl)piperazin-1-yl]methyl}benzonitrile (17)




To a 2-dram screw-cap vial was added PS-DIEA (54 mg, 0.2 mmol) and DMF (2 mL). The vials were placed in an orbital shaker allowed to shake for 15 min. 1-(2-phenylethyl)-piperazine (38 mg, 0.2 mmol was dissolved in DMF (100 mL) and transferred by pipette to the vial. 3-Chloromethylbenzonitrile (30 mg, 0.2 mmol), was dissolve in DMF (100 mL) and transferred to the vial. The vial was capped and heated at 80° C. with shaking for 12 h. The vial was cooled to RT and the reaction mixture filtered and solvent evaporated to give the desired substituted piperazine. MS ELS+ 306.
Intermediate Compound
2-(2,4-dichlorophenyl)ethyl methanesulfonate (18)




To a stirred mixture of 2,4-dichlorophenethylalcohol (955.0 mg, 5.0 mol) at 0° C. was added Et3N (1.1 mL, 7.5 mmol) and stirred for 5 min. Methane sulfonyl chloride (572.8 mg, 5.0 mmol) was added neat, dropwise by syringe. A precipitate formed and the reaction mixture stirred 30 min. The mixture was filtered, diluted with Et2O (20 mL) and cooled at to 0° C. After 20 min., the mixture was filtered through a pad of silica, dried over anhydrous Na2SO4 and evaporated. The crude residue was co-evaporated with toluene and chloroform (1:1) to give a yellow oil (1.1 g, 80%). The mesylate was used directly in subsequent reactions. 1H NMR (CDCl3) δ 2.9 (s, 3H), 3.18 (t, 2H), 4.42 (t, 2H), 7.22 (d, 2H), 7.41 (t, 1H).
Intermediate Compound
1-[(2-(2,4-dichlorophenyl)ethyl]piperazine (19)




Piperazine (1.9 g, 22 mmol) was stirred in 20 mL CH3CN at 75° C. Compound 19 (1.1 g, 4.1 mmol) was dissolved in CH3CN (2 mL) and added dropwise via syringe. The reaction mixture was heated at 60° C. for 30 min. The mixture was poured over ice and extracted with EtOAC. The combined organic layers were washed with water, dried over anhydrous magnesium sulfate (MgSO4), and evaporated to yield the desired piperazine as a yellow oil (826 mg, 80%). 1H NMR (CDCl3) δ 2.55 (bm, 6H), 2.9 (bm, 6H), 7.19 (d, 2H), 7.36 (t, 1H)
Example V
tert-butyl 4-(2-phenylpropanoyl)piperazine-1-carboxylate (20)




To a stirred mixture of 2-phenyl propionic acid (1.0 g, 6.7 mmol) in 1,2-dichloroethane (15 mL) was added a few drops of DMF and excess thionyl chloride. The resulting mixture was heated at reflux for 2 h, when bubbling ceased the mixture was evaporated to dryness. The crude residue was co-evaporated with toluene and 1,2-dichloroethane. The residue was dissolved in CH2Cl2 and cooled to 0° C. To the stirred solution, BOC-piperazine (1.24 g, 6.7 mmol) and Et3N were added. A white precipitate formed and the reaction was stirred 12 h at RT. Et2O was added and the mixture was washed with water and brine. The ether layer was separated and dried over anhydrous Na2SO4, filtered, and evaporated. The residue was treated with 20% ether/hexanes and the resulting solid filtered. The filtrate was evaporated and the procedure repeated. After drying, there remained a white solid (1.3 g, 62%). 1H NMR (CDCl3) δ: 1.4 (s, 9H), 1.42 (d, 3H), 2.7 (m, 1H), 3.1-3.6 (m, 6H), 3.85 (m, 2H), 7.2-7.4 (m, 5H). [M+Na] 341.
Example VI
1-[(6-chloro-1,3-benzodioxol-5-yl)methyl]-4-(2-phenylpropanoyl)piperazine (21)




To a stirred mixture of compound 201 (500 mg, 1.57 mmol) in CH2Cl2 was added trifluoroacetic acid TFA (excess). The mixture was stirred 1 h and was evaporated to dryness. The crude residue was dissolved in DMF and 6-chloropiperonyl chloride (321 mg, 1.57 mmol), and Et3N were added. The reaction mixture was stirred at 80° C. for 3 h after which the mixture was poured into water and extracted with Et2O. The combined Et2O layers were washed with water, brine and dried over anhydrous Na2SO4, filtered, and evaporated to give orange oil (500 mg, 83%). 1H NMR (CDCl3) δ: 1.42 (d, 3H), 1.95 (m, 1H), 2.25-2.5 (m, 3H), 3.2-3.8 (bm, 4H), 3.41 (s, 2H), 3.85 (q, 1H), 5.95 (s, 2H), 6.79 (s, 1H), 6.9 (s, 1H), 7.2-7.35 (m, 5H).
Example VII
1-[(6-chloro-1,3-benzodioxol-5-yl)methyl]-4-(2-phenylpropyl)piperazine (22)




To a stirred mixture of compound 21 (200, mg 0.52 mmol) in THF (7.0 mL) was added lithium aluminum hydride (LAH) (0.4 mL of 1M in THF). The mixture was refluxed for 1 h. To the cooled mixture was added ice-water to quench unreacted LAH. The mixture was stirred 1 h and extracted with Et2O and the combined organic layers were washed with brine, dried over anhydrous Na2SO4, and evaporated. The crude residue was purified via flash column (20% EtOAc/Hexanes) and the product fractions evaporated to give an orange oil (179.8 mg, 93%). 1H NMR (CDCl3) δ: 1.15 (d, 3H), 2.35-2.55 (bm, 10H), 2.9 (m, 1H), 3.5 (s, 2H), 5.98 (s, 2H), 6.8 (s, 1H), 6.95 (s, 1H) 7.18-7.35 (m, 5H). GC-MS: calcd, 372; found, 372.
TABLE 1 lists compounds of the invention prepared according to the procedures described in Scheme A-D as well as illustrated by Examples I-VII.









TABLE 1





Compound #
Structure
Characterization

















23




[M + H]+: 288


 


24




[M + H]+: 306


 


25




[M + H]+: 294


 


26




[M + H]+: 355


 


27




[M + H]+: 343


 


28




[M + H]+: 316


 


29




[M + H]+: 304


 


30




[M + H]+: 325


 


31




[M + H]+: 313


 


32




[M + H]+: 282


 


33




[M + H]+: 270


 


34




[M + H]+: 271


 


35




[M + H]+: 259


 


36




[M + H]+: 295


 


37




[M + H]+: 283


 


38




[M + H]+: 329, 331


 


39




[M + H]+: 317


 


40




[M + H]+: 311


 


41




[M + H]+: 299


 


42




[M + H]+: 306


 


43




[M + H]+: 294


 


44




[M + H]+: 315, 317


 


45




[M + H]+: 303


 


46




[M + H]+: 339


 


47




[M + H]+: 327


 


48




[M + H]+: 299


 


49




[M + H]+: 287


 


50




[M + H]+: 321


 


51




[M + H]+: 309


 


52




[M + H]+: 301


 


53




[M + H]+: 289


 


54




[M + H]+: 261


 


55




[M + H]+: 249


 


56




[M + H]+: 309


 


57




[M + H]+: 331, 333


 


58




[M + H]+: 393


 


59




[M + H]+: 339


 


60




[M + H]+: 407


 


61




[M + H]+: 353


 


62




[M + H]+: 339


 


63




[M + H]+: 369


 


64




[M + H]+: 437


 


65




[M + H]+: 383


 


66




[M + H]+: 391


 


67




[M + H]+: 413


 


68




[M + H]+: 359


 


69




[M + H]+: 345


 


70




[M + H]+: 375


 


71




[M + H]+: 326


 


72




[M + H]+: 396


 


73




[M + H]+: 345


 


74




[M + H]+: 360


 


75




[M + H]+: 407


 


76




[M + H]+: 353


 


77




[M + H]+: 353


 


78




[M + H]+: 299


 


79




[M + Na]: 337


 


80




[M + H]+: 399


 


81




[M + H]+: 389


 


82




[M + H]+: 383


 


83




[M + H]+: 384, 386


 


84




[M + H]+: 329


 


85




[M + H]+: 315


 


86




[M + H]+: 427, 430


 


87




[M + H]+: 407


 


88




[M + H]+: 439


 


89




[M + H]+: 386


 


90




[M + H]+: 339


 


91




[M + H]+: 365


 


92




[M + H]+: 373


 


93




[M + H]+: 357


 


94




[M + H]+: 345


 


95




[M + H]+: 413


 


96




[GC-MS]: 357


 


97




[M + H]+: 407


 


98




[M + H]+: 393


 


99




[M + H]+: 454


 


100




[M + H]+: 407


 


101




[GC-MS]: 382


 


102




[GC-MS]: 364


 


103




[M + H]+: 375


 


104




[GC-MS]: 358


 


105




[M + H]+: 405


 


106




[GC-MS]: 358


 


107




[GC-MS]: 3927


 


108




[M + H]+: 443


 


109




[M + H]+: 355


 


110




[M + H]+: 358


 


111




[M + H]+: 403


 


112




[M + H]+: 387


 


113




[M + H]+: 421


 


114




[M + H]+: 351


 


115




[M + H]+: 437


 


116




[M + H]+: 369


 


117




[M + H]+: 315


 


118




[M + H]+: 345


 


119




[M + H]+: 414


 


120




[M + H]+: 369


 


121




[M + H]+: 315


 


122




[M + H]+: 301


 


123




[M + H]+: 331


 


124




[M + H]+: 381


 


125




[M + H]+: 327


 


126




[M + H]+: 446


 


127




[M + H]+: 339


 


128




[M + H]+: 385


 


129




[M + H]+: 380


 


130




[M + H]+: 368


 


131




[M + H]+: 299


 


132




[M + H]+: 366


 


133




[M + H]+: 288


 


134




[M + H]+: 305


 


135




[M + H]+: 287


 


136




[M + H]+: 275


 


137




[M + H]+: 300


 


138




[M + H]+: 297


 


139




[M + H]+: 355


 


140




[M + H]+: 398


 


141




[M + H]+: 330


 


142




[M + H]+: 285


 


143




[M + H]+: 475


 


144




[M + H]+: 407


 


145




[M + H]+: 301


 


146




[M + H]+: 331


 


147




[M + H]+: 313


 


148




[M + H]+: 343


 


149




[M + H]+: 378


 


150




[M + H]+: 392


 


151




[M + H]+: 378


 


152




[M + H]+: 408


 


153




[M + H]+: 410


 


154




[M + H]+: 356


 


155




[M + H]+: 342


 


156




[M + H]+: 414


 


157




[M + H]+: 360


 


158




[M + H]+: 346


 


159




[M + H]+: 376


 


160




[M + H]+: 437


 


161




[M + H]+: 382


 


162




[M + H]+: 369


 


163




[M + H]+: 399


 


164




[M + H]+: 368


 


165




[M + H]+: 314


 


166




[M + H]+: 300


 


167




[M + H]+: 405


 


168




[M + H]+: 351


 


169




[M + H]+: 337


 


170




[M + H]+: 367


 


171




[M + H]+: 349


 


172




[M + H]+: 335


 


173




[M + H]+: 365


 


174




[M + H]+: 371


 


175




[M + H]+: 317


 


176




[M + H]+: 303


 


177




[M + H]+: 333


 


178




[M + H]+: 379


 


179




[M + H]+: 311


 


180




[M + H]+: 341


 


181




[M + H]+: 353


 


182




[M + H]+: 369


 


183




[M + H]+: 437


 


184




[M + H]+: 383


 


185




[M + H]+: 369


 


186




[M + H]+: 399


 


187




[M + H]+: 331


 


188




[M + H]+: 361


 


189




[M + H]+: 312


 


190




[M + H]+: 330


 


191




[M + H]+: 316


 


192




[M + H]+: 346


 


193




[M + H]+: 365


 


194




[M + H]+: 351


 


195




[M + H]+: 381


 


196




[M + H]+: 373


 


197




[M + H]+: 359


 


198




[M + H]+: 389


 


199




[M + H]+: 353


 


200




[M + H]+: 369


 


201




[M + H]+: 417


 


202




[M + H]+: 349


 


203




[M + H]+: 379


 


204




[M + H]+: 447


 


205




[M + H]+: 393


 


206




[M + H]+: 379


 


207




[M + H]+: 409


 


208




[M + H]+: 261


 


209




[M + H]+: 368


 


210




[M + H]+: 314


 


211




[M + H]+: 300


 


212




[M + H]+: 330


 


213




[M + H]+: 323


 


214




[M + H]+: 309


 


215




[M + H]+: 339


 


216




[M + H]+: 383


 


217




[M + H]+: 329


 


218




[M + H]+: 315


 


219




[M + H]+: 345


 


220




[M + H]+: 335


 


221




[M + H]+: 321


 


222




[M + H]+: 351


 


223




[M + H]+: 397


 


224




[M + H]+: 343


 


225




[M + H]+: 329


 


226




[M + H]+: 359


 


227




[M + H]+: 400


 


228




[M + H]+: 346


 


229




[M + H]+: 332


 


230




[M + H]+: 362


 


231




[M + H]+: 348


 


232




[M + H]+: 364


 


233




[M + H]+: 360


 


234




[M + H]+: 346


 


235




[M + H]+: 376


 


236




[M + H]+: 371


 


237




[M + H]+: 401


 


238




[M + H]+: 277


 


239




[M + H]+: 302


 


240




[M + H]+: 290


 


241




[M + H]+: 310


 


242




[M + H]+: 308


 


243




[M + H]+: 388


 


244




[M + H]+: 386


 


245




[M + H]+: 307


 


246




[M + H]+: 305


 


247




[M + H]+: 307


 


248




[M + H]+: 332


 


249




[M + H]+: 340


 


250




[M + H]+: 338


 


251




[M + H]+: 358


 


252




[M + H]+: 346


 


253




[M + H]+: 364


 


254




[M + H]+: 294


 


255




[M + H]+: 293


 


256




[M + H]+: 318


 


257




[M + H]+: 324


 


258




[M + H]+: 309


 


259




[M + H]+: 334


 


260




[M + H]+: 322


 


261




[M + H]+: 342


 


262




[M + H]+: 340


 


263




[M + H]+: 347


 


264




[M + H]+: 372


 


265




[M + H]+: 360


 


266




[M + H]+: 380


 


267




[M + H]+: 378


 


268




[M + H]+: 354


 


269




[M + H]+: 379


 


270




[M + H]+: 387


 


271




[M + H]+: 387


 


272




[M + H]+: 353


 


273




[M + H]+: 378


 


274




[M + H]+: 366


 


275




[M + H]+: 386


 


276




[M + H]+: 384


 


277




[M + H]+: 333


 


278




[M + H]+: 358


 


279




[M + H]+: 346


 


280




[M + H]+: 366


 


281




[M + H]+: 364


 


282




[M + H]+; 325


 


283




[M + H]+: 350


 


284




[M + H]+: 338


 


285




[M + H]+: 356


 


286




[M + H]+: 299


 


287




[M + H]+: 324


 


288




[M + H]+: 312


 


289




[M + H]+: 332


 


290




[M + H]+: 330


 


291




[M + H]+: 293


 


292




[M + H]+: 318


 


293




[M + H]+: 326


 


294




[M + H]+: 324


 


295




[M + H]+: 262


 


296




[M + H]+: 325


 


297




[M + H]+: 309


 


298




[M + H]+: 273


 


299




[M + H]+: 340


 


300




[M + H]+: 324


 


301




[M + H]+: 322


 


302




[M + H]+: 270


 


303




[M + H]+: 355


 


304




[M + H]+: 303


 


305




[M + H]+: 381


 


306




[M + H]+: 329


 


307




[M + H]+: 311


 


308




[M + H]+: 295


 


309




[M + H]+: 259


 


310




[M + H]+: 341


 


311




[M + H]+: 325


 


312




[M + H]+: 357


 


313




[M + H]+: 341


 


314




[M + H]+: 395


 


315




[M + H]+: 379


 


316




[M + H]+: 343


 


317




[M + H]+: 402


 


318




[M + H]+: 350


 


319




[M + H]+: 401


 


320




[M + H]+: 385


 


321




[M + H]+: 349


 


322




[M + H]+: 381


 


323




[M + H]+: 365


 


324




[M + H]+: 329


 


325




[M + H]+: 321


 


326




[M + H]+: 347


 


327




[M + H]+: 331


 


328




[M + H]+: 295


 


329




[M + H]+: 34


 


330




[M + H]+: 325


 


331




[M + H]+: 289


 


332




[M + H]+: 421


 


333




[M + H]+: 373


 


334




[M + H]+: 422









Example VIII
Insecticidal Testing
The compounds identified in TABLE 2 were prepared using the procedures illustrated in the previous examples and these compounds were tested against beet armyworm, mosquito, fruit fly, and cotton aphid using protocols described after.











TABLE 2





Compound #
LAPHEG
AEDSAE
DROSME
CA 200



















17
C
C
B
C


34
C
C
C
D


38
C
C
C
A


44
C
C
C
C


50
C
C
C
A


80
B
C
C
B


81
C
C
C
A


86
C
B
C
A


87
C
C
C
B


88
C
C
C
A


90
C
C
B
A


91
C
C
B
C


92
C
C
B
C


93
C
C
B
C


95
C
C
B
A


96
B
C
C
A


99
C
C
C
A


100
C
C
B
A


101
C
C
C
A


102
C
C
C
A


103
C
C
C
A


104
C
C
A
A


105
C
C
C
A


106
C
C
C
A


107
C
C
A
A


108
C
C
C
A


126
C
C
C
A


139
B
C
C
D


146
C
C
C
D


156
C
C
C
A


159
C
C
C
D


160
C
C
C
C


162
C
C
B
D


192
C
C
C
D


303
C
C
B
D


321
C
C
C
D


333
C
C
A
A





LAPHEG refers to % mortality at 4000 μg/ml against beet armyworm on artificial diet,


AEDSAE refers to % mortality at 20 μg/ml against mosquito larvae


DROSME refers to % mortality at 4000 μg/ml against adult fruit fly on artificial diet


CA 200 refers to % control at 200 ppm against cotton aphid in foliar spray tests.






In each case of Table 2 the rating scale is as follows:














% Mortality
Rating









50-100
A



26-49 
B



1-25
C



Not tested
D










Insecticidal Tests

Compounds were evaluated on mosquito (Aedes aegypti) larvae, beet armyworm (Spodoptera exigua) larvae, fruit fly (Drosophila melanogaster) adults and cotton aphid (Aphis gossypi). Newly hatched mosquito larvae (about 50 larvae/well) in 96-well microtiter plates in 230 ul of water containing powdered liver/yeast extract (1 gr/100 ml) were treated with 20 ppm solution of the test compound. Following treatment the plates were covered with a lid and mortality was determined three days after treatment. Beet armyworm eggs (about 5 per well) were placed on artificial diet (in a 96-well microtiter plate) treated with a 4000 ppm solution of a test compound in combination with piperonyl butoxide (in approximate ratio of 1:4). After infestation, the plates were covered with a lid and mortality was determined seven days following treatment. Five-day old fruit fly adults (about 5/well) were placed in deep-well microtiter plates containing 250 ul/well of a 2% agar-10% sucrose mixture that had been treated with a 4000 ppm solution of a test compound. Following infestation, the plates were covered with a lid and mortality was determined three days after treatment. Treatments for the mosquito and armyworm were replicated six times each, that of the fruit fly three times each.
Squash with fully expanded cotyledon leaves were trimmed to one cotyledon per plant and infested with cotton aphid (wingless adult and nymph) 1 day prior to chemical application.
Each plant is examined before chemical application to ensure proper infestation (ca. 30-70 aphids per plant). Compounds (3 mg) were dissolved in 3 mL of acetone:methanol (50:50) solvent, forming stock solutions of 1000 ppm. The stock solutions were then diluted with 0.025% Tween 20 (in H2O) to make 200 spray solutions. A hand-held Devilbiss sprayer was used to apply the spray solutions until runoff to both sides of the squash cotyledon leaves. Four plants (4 replications) were used for each concentration of each compound. Reference plants (solvent check) were sprayed with 0.025% Tween 20 only. Treated plants were held in a holding room for 3 days at approximately 23° C. and 40% RH before the number of live aphids on each plant was recorded. Insecticidal activity was measured by Corrected % Control using Abbott's correction formula and presented in TABLE 2:

Corrected % Control=100*(X−Y)/X

    
    
        where X=No. of live aphids on solvent check plants
        
            Y=No. of live aphids on treated plants
        
        
    
    


Example IX
Fungicidal Testing
The compounds identified in Table 4 were prepared using the procedures illustrated in the previous examples and these compounds were tested against wheat glume blotch and wheat leaf blotch using the protocols described herein.
Fungicidal Test for LEPTNO
Glume Blotch of Wheat (causal agent Leptosphaeria nodorum=Stagnospora nodorum; Bayer code LEPTNO): Wheat plants (variety Yuma) were grown from seed in a 50 pasteurized soil/50 percent soil-less mix until the seedlings had a fully expanded first leaf. Each pot contained 3-20 seedlings. These plants were sprayed until wet with the formulated test compound at a rate of 25 ppm. On the following day, the leaves were inoculated with an aqueous spore suspension of Leptosphaeria nodorum and the plants were kept in high humidity (one day in a dark dew chamber followed by four to seven days in a lighted dew chamber) to permit spores to germinate and infect the leaf. The plants were then transferred to a greenhouse until disease developed on untreated control plants.
Fungicidal Test for SEPTTR
Speckled Leaf Blotch of Wheat (Mycosphaerella graminicola=Septoria tritici; Bayer code SEPTTR): Wheat plants (variety Monon) were grown from seed in a greenhouse in 50% pasteurized soil/50% soil-less mix until the first true leaf was fully expanded, with 3-8 seedlings per pot. These plants were sprayed until wet with the formulated test compound at a rate of 25 ppm. On the following day, the leaves were inoculated with an aqueous spore suspension of Septoria tritici and the plants were kept in high humidity (one day in a dark dew chamber followed by four to seven days in a lighted dew chamber) to permit spores to germinate and infect the leaf The plants were then transferred to a greenhouse until disease developed on untreated control plants.









TABLE 3





Compound #
LEPTNO
SEPTTR

















80
A
A


81
A
A


88
C
A


92
A
C


93
C
A


109
C
A


110
C
A


111
A
A


112
C
A


114
C
A


118
A
A


119
A
B


128
A
A


187
A
A


234
A
C


236
A
C





LETPNO refers to in vitro control one day protectant at 25 ppm on wheat glume blotch


SEPTTR refers to in vitro control one day protectant at 25 ppm on wheat leaf blotch






In each case of TABLE 3 the rating scale is as follows:














% Control
Rating









50-100
A



26-49 
B



1-25
C

## Claims

1. A method for controlling a fungal attack said method comprising applying to the soil, plant, roots, foliage, seed or locus of the fungus, a fungicidally effective amount of a composition comprising a compound having the following formula



wherein
X and Y are each independently selected from the group consisting of a direct bond, alkyl, carbonyl, carbonylalkyl, carbonylalkylether, carboxylalkyl, alkylcarbonyl, alkylcarboxyl, and alkylether;
with the proviso that both X and Y cannot be a direct bond; and when either X or Y is a direct bond, then the R1 or R2 associated with that bond is not phenyl or optionally substituted phenyl; and
R1 and R2 are each independently selected from the group consisting of:
alkyl;
aryl, optionally substituted with cyano, alkoxy, halogen, alkylhalo, alkoxyhalo, carboxylalkyl, carbonylalkyl, benzyloxy, nitro, benzoyl, phenyl, carboxamido, or heteroaryl;
heteroaryl, optionally substituted with aryl, heteroaryl, alkyl, halogen, alkylhalo, alkoxy, cycloalkyl or cyano;
cycloalkyl, optionally substituted with alkyl or halogen; and
a heterocyclic ring, optionally substituted with alkyl
in a mixture with one or more fungicides or insecticides or both.