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# Time-resolved SAC-IR absorption spectra of insulin solutions 

## Appears In

                        - Unfolding and Aggregation of Therapeutic Proteins

Figure 2. Time-resolved SAC-IR absorption spectra of insulin solutions at (a) 17.5 mg/mL and (b) 3.5 mg/mL concentrations at 74 °C and pH = 2 [2]. Each spectrum is baseline detrended by absorbances at 1480 and 1750 cm-1. (c) and (d) Peak fitting of the amide I band with two Gaussian functions. (e) and (f) Areas of the two underlying peaks plotted as a function of time.

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