# Patent

## Title
Chemokine receptor modulators

## Abstract

The invention provides compounds of Formula (I)




and pharmaceutically acceptable salts, solvates, tautomers, stereoisomers, and/or esters thereof. These compounds, and pharmaceutical composition comprising such compounds are useful treating or preventing HIV infections, and in treating proliferative disorders such as inhibiting the metastasis of various cancers.

## Background
REFERENCE TO RELATED APPLICATIONS
This application claims priority to U.S. Provisional Application No. 61/040,516, filed Mar. 28, 2008, the disclosure of which is hereby incorporated by reference in its entirety.


FIELD OF THE INVENTION
The invention provides compounds, pharmaceutical compositions and methods of use of certain compounds that are antagonists of the chemokine receptor. The compounds are useful to modulate a medical condition that is mediated by chemokine receptor activity or signaling, such as diseases and disorders associated with reduced hematopoietic stem cell mobilization. The compounds can also be useful in the treatment or prevention of inflammation, the treatment or prevention of human immunodeficiency virus infections (HIV), or the diagnosis, prevention, and treatment of cancer or cancer related pathologies and side effects.
BACKGROUND
Chemokines are considered to be principal mediators in the initiation and maintenance of inflammation. This superfamily of small, secreted cytokines induce, through their interaction with G-protein-coupled receptors, cytoskeletal rearrangements and directional migration of several cell types (Butcher, et al. (1999) Adv Immunol 72: 209-253; Campbell and Butcher (2000) Curr Opin Immunol 12: 336-341; Zlotnik and Yoshie (2000) Immunity 12: 121-127). They have also been found to play an important role in the regulation of endothelial cell function, including proliferation, migration and differentiation during angiogenesis and re-endothelialization after injury (Gupta et al. (1998) J Biol Chem, 7:4282-4287).
The chemokine receptor, CXCR4, is known in viral research as a major coreceptor for the entry of T cell line-tropic HIV (Feng, et al. (1996) Science 272: 872-877; Davis, et al. (1997) J Exp Med 186: 1793-1798; Zaitseva, et al. (1997) Nat Med 3: 1369-1375; Sanchez, et al. (1997) J Biol Chem 272: 27529-27531). T Stromal cell derived factor 1 (SDF-1) is a naturally occurring chemokine that interacts specifically with CXCR4. When SDF-1 binds to CXCR4, CXCR4 activates Gα1-protein-mediated signaling (pertussis toxin-sensitive) (Chen, et al. (1998) Mol Pharmacol 53: 177-181), including downstream kinase pathways such as Ras/MAP Kinases and phosphatidylinositol 3-kinase (PI3K)/Akt in lymphocyte, megakaryocytes, and hematopoietic stem cells (Bleul, et al. (1996) Nature 382: 829-833; Deng, et al. (1997) Nature 388: 296-300; Kijowski, et al. (2001) Stem Cells 19: 453-466; Majka, et al. (2001) Folia. Histochem. Cytobiol. 39: 235-244; Sotsios, et al. (1999) J. Immunol. 163: 5954-5963; Vlahakis, et al. (2002) J. Immunol. 169: 5546-5554). In mice transplanted with human lymph nodes, SDF-1 induces CXCR4-positive cell migration into the transplanted lymph node (Blades et al. (2002) J. Immunol. 168: 4308-4317). These results imply that the interaction between SDF-1 and CXCR4 directs cells to the organ sites with high levels of SDF-1.
Studies have shown that CXCR4 interactions may regulate the migration of metastatic cells. Hypoxia, a reduction in partial oxygen pressure, is a microenvironmental change that occurs in most solid tumors and is a major inducer of tumor angiogenesis and therapeutic resistance. Hypoxia increases CXCR4 levels (Staller, et al. (2003) Nature 425: 307-311). Microarray analysis on a sub-population of cells from a bone metastatic model with elevated metastatic activity showed that one of the genes increased in the metastatic phenotype was CXCR4. Furthermore, overexpression of CXCR4 in isolated cells significantly increased the metastatic activity (Kang, et al. (2003) Cancer Cell 3: 537-549). In samples collected from various breast cancer patients, Muller et al. (Muller, et al. (2001) Nature 410: 50-56) found that CXCR4 expression level is higher in primary tumors relative to normal mammary gland or epithelial cells. These results suggest that the expression of CXCR4 on cancer cell surfaces may direct the cancer cells to sites that express high levels of SDF-I. Consistent with this hypothesis, SDF-1 is highly expressed in the most common destinations of breast cancer metastasis including lymph nodes, lung, liver, and bone marrow. Moreover, CXCR4 antibody treatment has been shown to inhibit metastasis to regional lymph nodes when compared to control isotypes that all metastasized to lymph nodes and lungs (Muller, et al. (2001)).
In addition to regulating migration of cancer cells, CXCR4-SDF-1 interactions may regulate vascularization necessary for metastasis. Blocking either CXCR4/SDF-1 interaction or the Gα1 signaling inhibits VEGF-dependent neovascularization. These results indicate that SDF-1/CXCR4 controls signaling systems that are regulators of endothelial cell morphogenesis and angiogenesis. Numerous studies have shown that VEGF and MMPs actively contribute to cancer progression and metastasis.
Cancer is currently the second leading cause of death in developed nations. In 2008, the American Cancer Society estimated that approximately 1.43 million new cases were diagnosed in the U.S. alone, and approximately 565,000 deaths occurred due to cancer (American Cancer Society, Cancer Facts & Figures 2008). Metastasis, the spread and growth of tumor cells to distant organs, is the most devastating attribute of cancer. Most morbidity and mortality associated with certain types of cancer, such as breast cancer, is associated with disease caused by metastatic cells rather than by the primary tumor. Therapy for metastasis currently relies on a combination of early diagnosis and aggressive treatment of the primary tumor.
The establishment and growth of metastases at distant sites is thought to depend on interactions between tumor cells and the host environment. Although a number of mediators have been implicated in the metastasis of cancers such as breast cancer, the precise mechanisms determining the directional migration and invasion of tumor cells into specific organs remain to be established. An incomplete understanding of the molecular and cellular mechanisms underlying metastasis has hindered the development of effective therapies that would eliminate or ameliorate this condition.
Several strategies have been developed to reduce metastatic invasion of malignant cells by regulating adhesion of endothelial cells with antibodies or adhesion molecules (see for example, PCT Publication No. WO 97/00956, U.S. Pat. Nos. 5,993,817; 6,433,149; 6,475,488; and 6,358,915). However, no commercial strategy has provided an effective treatment to prevent metastasis.
As of the end of 2007, an estimated 33 million people worldwide were living with HIV/AIDS, and the Centers for Disease Control and Prevention (CDC) estimate that 1,200,000 U.S. residents are living with HIV infection (UNAIDS/WHO AIDS epidemic update, December 2008; The Henry J. Kaiser Family Foundation HIV/AIDS Policy Fact Sheet, July 2007). Although new infections have decreased in recent years, an estimated 2.6 million new HIV infections occurred worldwide during 2007 and approximately 40,000 new HIV infections occur each year in the United States.
HIV entry within the target cells involves a series of molecular events. The three main steps of virus entry within the cell are: (i) attachment of the virus to the host cells; (ii) interaction of the virus with the co-receptors; (iii) fusion of the virus and host cell membranes. Considering the complexity of the molecular events involved in viral infection, all three of these steps have been considered for the drug design of HIV entry inhibitors. The T-lymphocyte cell surface protein CD4 is the primary receptor involved in the interaction with the viral glycoprotein gp120, but a cellular co-receptor is also needed for the successful entry of the virus within the cell. At least two types of such co-receptors have been identified so far, both of which are chemokine receptors. These chemokine receptors are therefore gateways for HIV entry, determinants of viral tropism and sensitivity.
Compounds targeting CXCR4 have been developed primarily for treatment of HIV because CXCR4 is a major coreceptor for T-tropic HIV infection. For example, U.S. Pat. No. 6,429,308 to Hisamitsu Pharmaceutical Co., Inc. discloses an antisense oligonucleotide to CXCR4 to inhibit the expression of the CXCR4 protein for use as an anti-HIV agent. PCT Publication No. WO 01/56591 to Thomas Jefferson University describes peptide fragments of viral macrophage inflammatory protein II which are described as selectively preventing CXCR4 signal transduction and coreceptor function in mediating entry of HIV-1.
Peptide antagonists of CXCR4 receptors have also been disclosed. Tamamura et al. (Tamamura, et al. (2000) Bioorg. Med. Chem. Lett. 10: 2633-2637; Tamamura, et al. (2001) Bioorg. Med. Chem. Lett. 11: 1897-1902) reported the identification of a specific peptide-based CXCR4 inhibitor, T140. T140 is a 14-residue peptide that possessed high levels of anti-HIV activity and antagonism of T cell line-tropic HIV-1 entry among all antagonists of CXCR4 (Tamamura, et al. (1998) Biochem. Biophys. Res. Commun. 253: 877-882). The compound has been altered to increase its efficacy and bioavailability by, for example, amidating the C-terminal of T-140 and reducing the total positive charges by substituting basic residues with nonbasic polar amino acids to generate TN14003, which is less cytotoxic and more stable in serum compared to T140. The concentration of TN14003 required for 50% protection of HIV-induced cytopathogenicity in MT-4 cells is 0.6 nM in contrast to 410 mM leading to 50% toxicity. U.S. Pat. No. 6,344,545 to Progenics Pharmaceuticals, Inc. describes methods for preventing HIV-1 infection of CD4+ cells with peptide fragments. U.S. Pat. No. 6,534,626 to the U.S. Department of Health & Human Services describes certain peptide chemokine variants for treating HIV infections. PCT Publication No. WO 04/087068 to Emory University describes CXCR4 peptide antagonists, particularly TN14003, and methods of their use to treat metastasis.
Other peptide-based antagonists have also been disclosed. For example, European Patent Publication Nos. 1286684 and 1061944 to the University of British Columbia cover methods of treatment of diseases, including metastasis, using modified peptide CXCR4 antagonists derived from the native SDF-1 ligand. PCT Publication No. WO 04/020462 to Takeda Chemical Industries, Ltd. provides peptide CXCR4 antagonists for treatment and prevention of breast cancer and chronic rheumatoid arthritis. U.S. Patent Application No. 2004/0132642 to the U.S. Dept. of Health & Human Services in part covers methods of inhibiting metastasis or growth of a tumor cell with a polypeptide CXCR4 inhibitor.
Several groups have now also identified chemokines including CXCR4 as a target for treatment of metastatic cancers. For example, PCT Publication Nos. WO 01/38352 to Schering Corporation, WO 04/059285 to Protein Design Labs, Inc., and WO 04/024178 to Burger generally describe methods of treating diseases and specifically inhibiting metastasis by blocking chemokine receptor signaling.
Although advances have been made, inadequate absorption, distribution, metabolism, excretion or toxicity properties of peptide inhibitors have limited their clinical use. Small non-peptide drugs remain a major goal of medicinal chemistry programs in this area.
At the present time, the metal-chelating cyclams and bicyclams represent one of the few reported non-peptide molecules to effectively block CXCR4 (Onuffer and Horuk (2002) Trends Pharmacol Sci 23: 459-467.36). One of these non-peptide molecules is AMD3100, which entered clinical trials as an anti-HIV drug that blocks CXCR4-mediated viral entry (Donzella, et al. (1998) Nat Med 4: 72-77; Hatse, et al. (2002) FEBS Lett 527: 255-262; Fujii, et al. (2003) Expert Opin Investig Drugs 12: 185-195; Schols, et al. (1997) Antiviral Res 35: 147-156).




However, a clinical study showed cardiac-related side effect of AMD3100 which in part caused its withdrawal from clinical development (Scozzafava, et al. (2002) J Enzyme Inhib Med Chem 17: 69-7641); Hendrix, et al. (2004) Journal of Acquired Immune Deficiency Syndromes 37(2)).

Other nitrogen containing bicyclic molecules have also been developed as CXCR4 antagonists. European Patent Publication No. 1431290 and PCT Publication No. WO 02/094261 to Kureha Chemical Industry Co., Ltd cover CXCR4 inhibitors that are potentially useful in treating various diseases including HIV infection.
U.S. Patent Publication No. 2004/0254221 to Yamamazi, et al. also provides compounds and use thereof to treat various diseases including HIV infections that are CXCR4 antagonists. The compounds are of the general Formula:




in which A is A1-G1-N(R1)—; A1 is hydrogen or an optionally substituted, mono- or polycyclic, heteroaromatic or aromatic ring; G1 is a single bond or —C(R2)(R3)—; R1, R2, and R3 can be optionally substituted hydrocarbon groups; W is an optionally substituted hydrocarbon or heterocyclic ring; x is —C(═O)NH—; y is —C(═O)—; and D1 is hydrogen atom, alkyl with a polycyclic aromatic ring, or amine.

PCT Publication No. WO 00/56729 and U.S. Pat. No. 6,750,348 to AnorMED describe certain heterocyclic small molecule CXCR4 binding compounds, teaching that these are useful for the protection against HIV infection. The compounds are of the general Formula:




in which W can be a nitrogen or carbon atom; Y is absent or is hydrogen; R1 to R7 can be hydrogen or straight, branched or cyclic C1-6 alkyl; R8 is a substituted heterocyclic or aromatic group; Ar is an aromatic or heteroaromatic ring; and X is specified ring structure.

PCT Publication No. WO 2004/091518 to AnorMED also describes certain substituted nitrogen containing compounds that bind to CXCR4 receptors. The compounds are described as having the effect of increasing progenitor cells and/or stem cells, enhancing production of white blood cells, and exhibiting antiviral properties. PCT Publication No. WO 2004/093817 to AnorMED also discloses substituted heterocyclic CXCR4 antagonists which are described as useful to alleviate inflammatory conditions and elevate progenitor cells, as well as white blood cell counts. Similarly, PCT Publication No. WO 2004/106493 to AnorMED describes heterocyclic compounds that bind to CXCR4 and CCR5 receptors consisting of a core nitrogen atom surrounded by three pendant groups, wherein two of the three pendant groups are preferably benzimidazolyl methyl and tetrahydroquinolyl, and the third pendant group contains nitrogen and optionally contains additional rings. The compounds demonstrate protective effects against infections of target cells by a human immunodeficiency virus (HIV).
PCT Patent Application PCT/US06/000604, filed Jan. 9, 2006, describes certain compounds for the treatment of medical disorders mediated by CXCR4. These compounds include two nitrogen linked cyclic substituents off a central aromatic or cyclic alkyl or heteroalkyl.
In light of the fact that chemokine receptors are implicated in metastatic signaling as well as a number of other pathogenic conditions, it is important to identify new effective chemokine receptor modulators.
It is therefore an object of the invention to provide new compounds, methods and compositions that modulate chemokine receptors.
It is another object of the invention to provide compounds, methods and compositions that bind to chemokine receptors and interfere with their binding to their native ligands.
It is also an object of the invention to provide new compounds, methods and compositions for the treatment of viral infection, such as HIV, as well as for treatment of inflammatory and proliferative disorders.
SUMMARY
The present invention provides compounds and compositions that antagonize chemokine receptor signaling. The compounds described herein, are particularly useful for the treatment or prevention of a disorder associated with chemokine receptor binding or activation. These compounds can be used to treat diseases and disorders that are associated with chemokine activation, including diseases and disorders associated with reduced hematopoietic stem cell mobilization. Methods for the treatment of inflammatory, proliferative or infectious diseases and disorders mediated by chemokine signaling are also provided, including the prevention of symptoms associated with certain chemotherapeutics. The compounds and compositions are particularly useful in disorders associated with the chemokine CXCR4 receptor.
In general embodiments, the compounds, compositions and methods of the invention are of Formula (I)




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof, wherein:

R1 and R2 are each independently H, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted heteroalkyl including alkoxyalkyl, haloalkyl, CF3, halogen, hydroxy, amino, optionally substituted alkyl or dialkyl amino, optionally substituted alkoxy, hydroxyalkyl, optionally substituted aminoalkyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aralkyl, optionally substituted arylalkyl, carboxy, acyl, optionally substituted alkoxycarbonyl, or optionally substituted aminocarbonyl; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl;
M is H or -G-L3-Z;
E is H, —W-J-L2-Y,




L1, L2, and L3 are each independently selected from the group consisting of a covalent bond, substituted or unsubstituted alkylene, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted carbocyclyl, and substituted or unsubstituted heterocyclyl;
X and Y are independently H, NRaRb, —ORc, halogen, CF3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, acyl, carboxy, optionally substituted alkoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted alkyl- or dialkylaminocarbonyl, cyano, optionally substituted heterocyclylacyl, optionally substituted carbocyclylacyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl;
Y is selected from the group consisting of H, NRaRb, —ORc, substituted or unsubstituted carbocyclyl, and substituted or unsubstituted heterocyclyl;
Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or
Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl;
Rc is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl,
J is selected from the group consisting of a covalent bond, alkylene, —C(O)—NRd—, —C(O)—O—, —NRd—, and —C(O)—;
Rd is selected from the group consisting of H, alkyl, and substituted or substituted arylalkyl;
Q is CRe or N; Re is selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl;
G is selected from the group consisting of a covalent bond, alkylene, —C(O)—, —C(O)—O—, and —C(O)—NRd—;
W is selected from the group consisting of a covalent bond and a substituted or unsubstituted heterocyclyl;
R3 and R4 are each independently selected from the group consisting of H, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; or
R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl;
R5 and R6 are each independently selected from the group consisting of substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted heterocyclylalkyl, alkyl, alkenyl, and alkynyl; or
-L2-Y and R6, together with the atoms to which they are shown attached form a fused substituted or unsubstituted ring; or
E and -L1-X, together with the atoms to which they are shown bonded form a substituted or unsubstituted heterocyclyl;
R7 is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted carbocyclylalkyl, and substituted or unsubstituted heterocyclylalkyl; or
L2-Y and R7 together with the atoms to which they are shown bonded form a fused substituted or unsubstituted ring; and
A and B are each independently one or more substituents selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl;
with the proviso that only one of M, E and L1-X is H.
In certain embodiments, the present invention provides compounds, compositions and methods including the following Formulae:







or a pharmaceutically acceptable salts, solvates, tautomers, stereoisomers, and/or esters thereof, wherein variables are defined as for Formula I.

In certain more specific embodiments, the present invention provides compounds, compositions and methods including the following Formulae:




or a pharmaceutically acceptable salts, solvates, tautomers, stereoisomers, and/or esters thereof, wherein variables are defined as for Formula I. In some embodiments, the present invention is directed to a pharmaceutical composition comprising at least one compound of Formula (I), or a pharmaceutically acceptable salt, solvate, prodrug, tautomer, or ester thereof, and a pharmaceutically acceptable excipient.
In yet another embodiment, the present invention is directed to a pharmaceutical composition comprising at least one compound of Formula (I), or a pharmaceutically acceptable salt, solvate, prodrug, tautomer, or ester thereof, and a pharmaceutically acceptable excipient, and at least one additional pharmaceutically active compound.
In still another embodiment, the present invention is directed to a method of treating a disorder, symptom or disease in a patient in need of such treatment, comprising administering to the patient an effective amount of at least one compound of Formula (I). Typically, the methods of use of the present invention are directed towards treatment or prophylaxis of a disorder, symptom or disease that is modulated by chemokine receptor activity or signaling. Disorders to be treated generally are those in which inhibition of chemokine signaling, particularly that of a CXCR4 receptor, is desirable. In certain embodiments, the compounds are administered to a patient at risk of or suffering from a disorder causing a reduction in lymphocytes or myeloid cells.


DETAILED DESCRIPTION OF THE INVENTION
The compounds, methods, and compositions of the present invention can modulate the effect of chemokine receptors. These compounds can be used to treat diseases and disorders that are associated with CXCR4 activation, including diseases and disorders associated with reduced hematopoietic stem cell mobilization. In addition, methods of treatment of disorders including tumor metastasis or any other disease, particularly hyperproliferative diseases involving chemokine receptors are provided. The compounds can also be used to treat or prevent HIV infection, reduce viral load, or alleviate progression towards, or the symptoms of, AIDS in a host in need thereof.
Compounds described herein have the capacity to interact with chemokine receptors and potentially inhibit receptor signaling. The compounds of the present invention have increased bioavailability and efficacy in inhibiting chemokine receptors.
Although not bound by theory, these compounds may inhibit certain disorders such as metastasis through their capacity to inhibit SDF-1-chemokine receptor interactions, which can decrease cell targeting, and may also reduce VEGF-dependent endothelial cell morphogenesis and angiogenesis. This endothelial cell growth is a key event in metastases of tumors.
DEFINITIONS
The term “organism,” “host,” or “patient” are used interchangeably to refer to any living entity comprised of at least one cell. A living organism can be as simple as, for example, a single eukaryotic cell or as complex as a mammal, including a human being. In certain embodiments, the organism is a human in need of treatment.
The term “chemokine receptor modulator” means a substance including but not limited to a molecule, polypeptide, polynucleotide, inhibitory polynucleotide, or siRNA, that interferes or inhibits the biological activity of the chemokine receptors including, but not limited to, the binding of a ligand to the receptor. The term “chemokine peptide antagonist” means a polypeptide that specifically binds to a chemokine receptor, particularly polypeptides that are not an antibody. Representative chemokine peptide antagonists include T140 and derivatives of T140. Exemplary derivatives of T140 include, but are not limited to, TN14003, TC14012, and TE14011 as well as those found in Tamamura, H. et al. Org. Biomol. Chem. 1:3656-3662, 2003, which is incorporated by reference herein in its entirety.
The term “therapeutically effective amount” or “effective amount”, as used herein, means an amount of a compound or composition which is sufficient enough to significantly and positively modify the symptoms and/or conditions to be treated (e.g., provide a positive clinical response). The effective amount of an active ingredient for use in a pharmaceutical composition will vary with the particular condition being treated, the severity of the condition, the duration of the treatment, the nature of concurrent therapy, and the particular active ingredient(s) being employed, and like factors within the knowledge and expertise of the attending physician. For example, in reference to cancer or pathologies related to unregulated cell division, a therapeutically effective amount refers to that amount which has the effect of (1) reducing the size of a tumor, (2) inhibiting (that is, slowing to some extent, preferably stopping) aberrant cell division, for example cancer cell division, (3) preventing or reducing the metastasis of cancer cells, and/or, (4) relieving to some extent (or, preferably, eliminating) one or more symptoms associated with a pathology related to or caused in part by unregulated or aberrant cellular division, including for example, cancer, or angiogenesis. With respect to stem cell mobilization, an effective amount is sufficient to improve improve stem cell mobilization as measured, for example, by an increase in the presence of and/or activity of blood cell types including those of myeloid (monocytes and macrophages, neutrophils, basophils, eosinophils, erythrocytes, megakaryocytes/platelets, dendritic cells), and/or lymphoid lineages (T-cells, B-cells, NK-cells). Representative dosage ranges and administrative regimes are further described below, however these can be subject to adjustment in individual patients. A “pharmaceutical composition” refers to a mixture of one or more of the compounds described herein, or pharmaceutically acceptable salts thereof, with other chemical components, such as physiologically acceptable carriers and excipients. One purpose of a pharmaceutical composition is to facilitate administration of a compound to an organism.
As used herein, a “pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” refers to a carrier or diluent that does not cause significant irritation to an organism and does not abrogate the biological activity and properties of the administered compound.
An “excipient” refers to an inert substance added to a pharmaceutical composition to further facilitate administration of a compound, such as binders, anti-adherents, coatings, disintegrants, fillers, diluents, flavors, colors, glidants, lubricants, preservatives, sorbitans, and sweeteners. Examples, without limitation, of excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils and polyethylene glycols.
“Treating” or “treatment” of a disease includes preventing the disease from occurring in an animal that may be predisposed to the disease but does not yet experience or exhibit symptoms of the disease (prophylactic treatment), inhibiting the disease (slowing or arresting its development), providing relief from the symptoms or side-effects of the disease (including palliative treatment), and relieving the disease (causing regression of the disease). With regard to HIV or cancer, these terms can mean that the life expectancy of an individual affected with HIV or cancer will be increased or that one or more of the symptoms of the disease will be reduced.
The term “prodrug” refers to an agent, including nucleic acids and polypeptides, which is converted into a biologically active form in vivo. Prodrugs are often useful because, in some situations, they may be easier to administer than the parent compound. They may, for instance, be bioavailable by oral administration whereas the parent compound is not. The prodrug may also have improved solubility in pharmaceutical compositions over the parent drug. A prodrug may be converted into the parent drug by various mechanisms, including enzymatic processes and metabolic hydrolysis. Harper, N. J. (1962). Drug Latentiation in Jucker, ed. Progress in Drug Research, 4:221-294; Morozowich et al. (1977). Application of Physical Organic Principles to Prodrug Design in E. B. Roche ed. Design of Biopharmaceutical Properties through Prodrugs and Analogs, APhA; Acad. Pharm. Sci.; E. B. Rocke, ed. (1977). Bioreversible Carriers in Drug in Drug Design, Theory and Application, APHA; H. Bundgaard, 15 ed. (1985) Design of Prodrugs, Elsevier; Wang et al. (1999) Prodrug approaches to the improved delivery of peptide drug, Curr. Pharm. Design. 5(4):265-287; Pauletti et al. (1997). Improvement in peptide bioavailability: Peptidomimetics and Prodrug Strategies, Adv. Drug. Delivery Rev. 27:235-256; Mizen et al. (1998). The Use of Esters as Prodrugs for Oral Delivery of P-Lactam antibiotics, Pharm. Biotech. 11:345-365; Gaignault et al. (1996). Designing Prodrugs and Bioprecursors I. Carrier Prodrugs, Pract. Med. Chem. 671-696; M. Asgharnejad (2000). Improving Oral Drug Transport Via Prodrugs, in G. L. Amidon, P. I. Lee and E. M. Topp, Eds., Transport Processes in Pharmaceutical Systems, Marcell Dekker, p. 185-218; Balant et al. (1990) Prodrugs for the improvement of drug absorption via different routes of administration, Eur. J. Drug Metab. Pharmacokinet., 15(2): 143-53; Balimane and Sinko (1999). Involvement of multiple transporters in the oral absorption of nucleoside analogues, Adv. Drug Delivery Rev., 39(1-3): 183-209; Browne (1997). Fosphenytoin (Cerebyx), Clin. Neuropharmacol. 20(1): 1-12; Bundgaard (1979). Bioreversible derivatization of drugs—principle and applicability to improve the therapeutic effects of drugs, Arch. Pharm. Chemi. 86(1): 1-39; H. Bundgaard, ed. (1985) Design of Prodrugs, New York: Elsevier; Fleisher et al. (1996). Improved oral drug delivery: solubility limitations overcome by the use of prodrugs, Adv. Drug Delivery Rev, 19(2): 115-130; Fleisher et al. (1985). Design of prodrugs for improved gastrointestinal absorption by intestinal enzyme targeting, Methods Enzymol. 112:360-81; Farquhar D, et al. (1983). Biologically Reversible Phosphate-Protective Groups, J. Pharm. Sci., 72(3): 24-325; Han, H. K. et al. (2000). Targeted prodrug design to optimize drug delivery, AAPS PharmSci., 2(1): E6; Sadzuka Y. (2000). Effective prodrug liposome and conversion to active metabolite, Curr. Drug Metab., 1(1):31-48; D. M. Lambert (2000) Rationale and applications of lipids as prodrug carriers, Eur. J. Pharm. Sci, 11 Suppl2:S15-27; Wang, W. et al. (1999) Prodrug approaches to the improved delivery of peptide drugs. Gurr. Pharm. Des., 5(4):265-87.
The term “alkyl”, as used herein, unless otherwise specified, includes but is not limited to a saturated straight or branched, primary, secondary, or tertiary hydrocarbon of C1 to C20 or C1 to C10 and specifically includes methyl, ethyl, propyl, isopropyl, cyclopropyl, butyl, isobutyl, t-butyl, pentyl, cyclopentyl, isopentyl, neopentyl, hexyl, isohexyl, cyclohexyl, cyclohexylmethyl, 3-methylpentyl, 2,2-dimethylbutyl, and 2,3-dimethylbutyl. The term optionally includes substituted alkyl groups. Moieties with which the alkyl group can be substituted are selected from the group consisting of halo (e.g., trifluoromethyl), hydroxyl, amino, alkylamino, arylamino, alkoxy, aryloxy, nitro, cyano, sulfonic acid, sulfate, phosphonic acid, phosphate, or phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art, for example, as taught in Greene, et al., Protective Groups in Organic Synthesis, John Wiley and Sons, Second Edition, 1991, hereby incorporated by reference.
The term “alkenyl” refers to an alkyl, as defined herein, in which at least one C—C (single) bond is replaced with a C═C (double) bond. The alkenyl can be branched or straight chain, and can have one, two or more C═C double bonds, which can be conjugated or unconjugated.
The term “alkynyl” refers to an alkyl, as defined herein, in which at least one C—C (single) bond is replaced with a C≡C (triple) bond. The alkynyl can be branched or straight chain, and can have one, two or more C≡C triple bonds.
Whenever the terms “C1-C5 alkyl”, “C2-C5 alkenyl”, “C1-C5 alkoxy”, “C2-C5 alkenoxy”, “C2-C5 alkynyl”, and “C2-C5 alkynoxy”, “C3-C5” are used, these are considered to include, independently, each member of the group, such that, for example, C1-C5 alkyl includes straight, branched and C1, C2, C3, C4 and C5 alkyl functionalities; C2-C5 alkenyl includes straight and branched C2, C3, C4 and C5 alkenyl functionalities; C1-C5 alkoxy includes straight and branched, C1, C2, C3, C4 and C5 alkoxy functionalities; C2-C5 alkenoxy includes straight and branched C2, C3, C4 and C5 alkenoxy functionalities; C2-C5 alkynyl includes straight and branched C1, C2, C3, C4 and C5 alkynyl functionalities; and C2-C5 alkynoxy includes straight and branched C2, C3, C4 and C5 alkynoxy functionalities, etc.
The term “lower alkyl”, as used herein, and unless otherwise specified, includes a C1 to C4 saturated straight or branched alkyl group, optionally including substituted forms. Unless otherwise specifically stated in this application, when alkyl is a suitable moiety, lower alkyl is preferred. Similarly, when alkyl or lower alkyl is a suitable moiety, unsubstituted alkyl or lower alkyl is preferred.
The term “alkylene”, as used herein, means an organic radical formed from an unsaturated aliphatic hydrocarbon. Typically, an alkylene can be represented by the following Formula: —C(RR′)n—, wherein n is an integer of one or more, and R and R′ is hydrogen, halo, hydroxyl, amino, cyano (i.e., —CN), nitro, alkoxy, alkylamino, arylamino, sulfate, sulfonic acid, phosphonic acid, phosphate, phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art. Preferably, n is an integer from 1 to 20. More preferably, n is an integer from 1 to 6. The alkylene can be straight, branched, or cyclic. Non-limiting examples of alkylene include —CH2— (methylene), —CH2CH2-(ethylene), —CH2CH2CH2— (propylene), etc.
The term “amino” includes an amine group (i.e., —NH2) as well as an amine group substituted with one or more alkyl groups, substituted alkyl groups (e.g., hydroxyalkyl, alkoxyalkyl, thioalkyl, alkylthioalkyl, etc.), one or two aryl groups, one or two heteroaryl groups, one or two arylalkyl groups, one or two heteroarylalkyl groups, combinations of H, alkyl, aryl, heteroaryl, arylalkyl, and heteroarylalkyl groups. When the amino group has one or more alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl groups, the alkyl, aryl, heteroaryl, arylalkyl, or heteroarylalkyl groups can be unsubstituted or substituted. The terms “alkylamino” or “arylamino” refer to an amino group that has one or two alkyl or aryl substituents, respectively. The terms “arylalkylamino” or “heteroarylalkylamino” refer to an amino group that has one or two arylalkyl or heteroaryl alkyl groups, respectively. In any instance in which the valence of a compound is drawn in error in this application, the valence is to be corrected by including and/or excluding a hydrogen.
The term “amino” can also include amino groups substituted with acyl groups such as —C(O)-alkyl, —C(O)-aryl, —C(O)-heteroaryl, —C(O)—O-alkyl, —C(O)—O-aryl, —C(O)—O-heteroaryl, —C(O)—N(R)-alkyl, —C(O)—N(R)-aryl, —C(O)—N(R)-heteroaryl; sulfonyl groups such as —S(O)2-alkyl, —S(O)2-aryl, —S(O)2-heteroaryl, —S(O)2—O-alkyl, —S(O)2—O-aryl, —S(O)2—O-heteroaryl, —S(O)2—N(R)-alkyl, —S(O)2—N(R)-aryl, —S(O)2—N(R)-heteroaryl, etc. (wherein R is H, alkyl, aryl, heteroaryl). When the substituent on the amino group is an acyl group, the moiety can also be referred to as an “amido” group (i.e., when the acyl group is —C(O)-alkyl, —C(O)-aryl, or —C(O)-heteroaryl), a “urea” moiety (i.e., when the acyl group is —C(O)—N(R)-alkyl, —C(O)—N(R)-aryl, or —C(O)—N(R)-heteroaryl), or a “urethane” moiety (i.e., when the acyl group is —C(O)—O-alkyl, —C(O)—O-aryl, or —C(O)—O-heteroaryl).
Unless stated to the contrary, a substituent is bound to a structure through the last named moiety of the substituent. For example, an “arylalkyl” substituent is bound to a structure through the “alkyl” moiety of the substituent.
The term “aminoalkyl”, as used herein, means an amino groups bonded to the parent moiety through an alkyl moiety (i.e., amino-alkyl-), wherein the amino and alkyl portions of the aminoalkyl are each as defined herein. Non-limiting examples of aminoalkyl include H2N—(CH2)2—CH2—, H2N—(CH2)3—CH2—, (CH3)2N—CH2CH2—, CH3—O—CH2CH2NH—CH2—, aryl-NH—(CH2)3—CH2—, heteroaryl-NH—(CH2)3—CH2—, H2N—C(O)—NH—(CH2)2—CH2—, etc. The term aminoalkyl can also refer to nitrogen containing heterocycles attached to an alkylene through the nitrogen atom of the heterocycle, e.g., pyrrolidine-CH2—, piperidine-CH2CH2—, morpholine-CH2CH2—, etc.
The term “amido”, “aminoacyl”, or “aminocarbonyl”, as used herein, means amino-C(O)—, wherein the amino moiety is any amino as defined herein. Non-limiting examples of aminoacyl include phenyl-NH—C(O)—, piperazine-C(O)—, pyrrolidine-C(O)—, (CH3—O—CH2CH2)2N—C(O)—, pyridine-CH2—NH—C(O)—, phenyl-CH2—NH—C(O)—, etc.
The term “aminoacylalkyl”, as used herein, means amino-C(O)-alkyl-, wherein the amino-C(O) moiety and alkyl moiety are as defined herein.
The term “arylamino”, as used herein, means aryl-amino-, wherein the amino moiety is any amino as defined herein. Non-limiting examples of arylamino include phenyl-NH—, halo substituted phenyl-NH—, etc.
The term “heteroarylamino”, as used herein, means heteroaryl-amino-, wherein the amino moiety is any amino as defined herein. Non-limiting examples of arylamino include pyrimidine-NH—, halo substituted pyrimidine-NH—, haloalkyl substituted pyrimidine-NH—, etc.
The term “protected” as used herein and unless otherwise defined refers to a group that is added to an oxygen, nitrogen, or phosphorus atom to prevent its further reaction or for other purposes. A wide variety of oxygen and nitrogen protecting groups are known to those skilled in the art of organic synthesis.
The term “aryl”, as used herein, means an aromatic monocyclic or multicyclic ring system comprising about 6 to about 14 carbon atoms, preferably about 6 to about 10 carbon atoms. Non-limiting examples of suitable aryl groups include phenyl, biphenyl, or naphthyl. The term aryl refers to unsubstituted aryl groups or aryl groups substituted with one or more substituents which may be the same or different. The aryl group can be substituted with one or more substituents, including but not limited to substituents selected from the group consisting of hydroxyl, thiol, amino, alkylamino, arylamino, alkoxy, aryloxy, nitro, cyano, sulfonic acid, halo (F, Cl, I, Br), carboxy, ester, acyl, alkyl (i.e., any of the alkyl groups described herein, such as methyl, ethyl, propyl, butyl, etc.), alkenyl (i.e., any of the alkenyl groups described herein, such as vinyl, allyl, 1-propenyl, 1-butenyl, 2-butenyl, etc.), alkynyl (i.e., any of the alkynyl groups described herein, such as 1-ethynyl, 1-propynyl, 2-propynyl, etc.), haloalkyl (i.e., any of the haloalkyl groups described herein), sulfate, sulfonate, sulfonic esters and amides, phosphoric acid, phosphonic acid, phosphate, or phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art, for example, as taught in Greene, et al., Protective Groups in Organic Synthesis, John Wiley and Sons, Second Edition, 1991.
The term “alkaryl” or “alkylaryl” refers to an alkyl group with an aryl substituent. In one embodiment, the “alk” or “alkyl” portion of the alkaryl is a lower alkyl group. Non-limiting examples of suitable alkylaryl groups include o-tolyl, p-tolyl and xylyl. The bond to the parent moiety is through the aryl.
The term “aralkyl” or “arylalkyl” refers to an aryl group attached to an alkyl group. In one embodiment, the “alk” or “alkyl” portion of the aralkyl is a lower alkyl group. Non-limiting examples of suitable aralkyl groups include benzyl, 2-phenethyl and naphthalenylmethyl. The bond to the parent moiety is through the alkyl. The aryl portion of the arylalkyl group may be substituted or unsubstituted.
The term “alkoxy”, as used herein, means alkyl-O—, wherein the alkyl moiety of the alkoxy group is an alkyl group as defined herein.
The term “cycloalkyl” means a non-aromatic mono- or multicyclic fused ring system comprising 3 to 10 ring carbon atoms, preferably 3 to 7 ring carbon atoms, more preferably 3 to 6 ring carbon atoms. The cycloalkyl can be optionally substituted with one or more substituents which may be the same or different. Non-limiting examples of suitable monocyclic cycloalkyls include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like. Non-limiting examples of suitable multicyclic cycloalkyls include 1-decalinyl, norbornenyl, adamantyl and the like. Suitable substituents for cycloalkyls include substituents selected from the group consisting of hydroxyl, thiol, amino, alkylamino, arylamino, alkoxy, aryloxy, nitro, cyano, sulfonic acid, halo (F, Cl, I, Br), carboxy, ester, acyl, alkyl (i.e., any of the alkyl groups described herein, such as methyl, ethyl, propyl, butyl, etc.), alkenyl (i.e., any of the alkenyl groups described herein, such as vinyl, allyl, 1-propenyl, 1-butenyl, 2-butenyl, etc.), alkynyl (i.e., any of the alkynyl groups described herein, such as 1-ethynyl, 1-propynyl, 2-propynyl, etc.), haloalkyl (i.e., any of the haloalkyl groups described herein), sulfate, sulfonate, sulfonic esters and amides, phosphoric acid, phosphonic acid, phosphate, or phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art, for example, as taught in Greene, et al., Protective Groups in Organic Synthesis, John Wiley and Sons, Second Edition, 1991, herein incorporated by reference in its entirety. Substituents can also include fused aromatic rings, e.g.:




wherein the fused aromatic or heteroaromatic ring can itself be unsubstituted or substituted with one or more substituents as described herein.

The term “halo”, as used herein, includes chloro, bromo, iodo, and fluoro.
The term “haloalkyl”, as used herein, means an alkyl as defined above wherein one or more hydrogen atoms on the alkyl are replaced by a halo defined above. Non-limiting examples of haloalkyl groups include —CF3, —CH2CF3, etc.
The term “hydroxyalkyl”, as used herein, means an alkyl group having at least one hydroxy substituent. Non-limiting examples of hydroxyalkyl groups include hydroxyethyl, 3-hydroxypropyl, 2-hydroxy propyl, etc.
The term “alkoxyalkyl”, as used herein, means alkyl-O-alkyl-, wherein each of the alkyl moieties is as defined herein. The skilled practitioner will recognize that a divalent alkyl group (i.e., an alkyl group bonded to two other moieties) can also be referred to as an “alkylene” group. An alkylene group is an alkyl group in which one of the C—H bonds is replaced with a covalent bond to another moiety. Non-limiting examples of alkoxyalkyl groups include CH3—O—CH2CH2—, CH3—O—CH2CH2CH2—, CH3CH2—O—CH2CH2—, CH3CH2—O—CH2CH2CH2—, t-Bu-O—CH2CH2—, etc.
The term “acyl” refers to a carbonyl group (—C(O)—). For example, arylacyl refers to groups such as phenyl-C(O)—, alkylacyl refers to acetyl, aminoacyl refers to H2N—C(O)— (wherein the N atom can be substituted with aryl, alkyl, heterocyclyl, etc), etc. When the acyl group forms, for example, a ketone, a carboxy group, a carbonate group, a urea group, a thio ester, etc, the non-carbonyl moiety of the such a group is selected from straight, branched, or cyclic alkyl or lower alkyl, alkoxyalkyl including methoxymethyl, aralkyl including benzyl, aryloxyalkyl such as phenoxymethyl, aryl including phenyl optionally substituted with halogen, C1 to C4 alkyl or C1 to C4 alkoxy, sulfonate esters such as alkyl or aralkyl sulphonyl including methanesulfonyl, the mono, di or triphosphate ester, trityl or monomethoxytrityl, substituted benzyl, trialkylsilyl (e.g. dimethyl-t-butylsilyl) or diphenylmethylsilyl. In one embodiment, aryl groups in the esters comprise a phenyl group. The term “lower acyl” refers to an acyl group in which the non-carbonyl moiety is a lower alkyl.
The term “carboxy”, as used herein, means —C(O)OH or an ester thereof.
The term “alkoxycarbonyl”, as used herein, means —C(O)—O-alkyl, wherein the alkyl moiety is any alkyl as defined herein.
The term “alkylthioalkyl”, as used herein, means alkyl-5-alkyl-, wherein each of the alkyl moieties is as defined herein. Non-limiting examples of alkylthioalkyl groups include CH3—S—CH2CH2—, CH3—S—CH2CH2CH2—, CH3CH2—S—CH2CH2—, CH3CH2—S—CH2CH2CH2—, t-Bu-S—CH2CH2—, etc.
The term “alkylamino”, as used herein, means alkyl-amino-, wherein the amino moiety can be any amino as defined herein.
The term “a 5- to 18-membered saturated heterocyclic ring containing at least one nitrogen atom” means a saturated monocyclic or multicyclic ring system comprising 5 to 18 atoms as the members constituting the ring system wherein the 5 to 18 atoms are chosen from carbon, nitrogen, sulfur, or phosphorous and at least one of the 5 to 18 atoms are nitrogen. The term encompasses a 6-18 membered saturated heterocyclic ring containing one or more, e.g., 2 nitrogen atoms. The multicyclic ring system can be fused or bridged multicyclic rings. The 5- to 18-membered saturated heterocyclic ring can optionally be substituted at any substitutable position (including at a heteroatom) by groups including substituted or unsubstituted alkyl (e.g., hydroxyalkyl, haloalkyl, alkoxyalkyl, etc.), halo, hydroxyl, oxo, amino (as defined herein, e.g., —NH2, amido, sulfonamido, urea moiety, urethane moiety), aminoacyl (as defined herein), aminoalkyl (as defined herein), amino-S(O)2—, alkyl-S(O)2—, arylamino (as defined herein), heteroarylamino (as defined herein), alkylamino (as defined herein), alkoxy, alkoxycarbonyl, aryloxy, nitro, cyano, aryl, heteroaryl, carboxy (as defined herein), sulfonic acid, sulfate, phosphonic acid, phosphate, or phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art. Non-limiting examples of substituted or unsubstituted 5- to 18-membered saturated heterocyclic rings containing at least one nitrogen atom include the following:




With reference to the number of moieties (e.g., substituents, groups or rings) in a compound, unless otherwise defined, the phrases “one or more” and “at least one” mean that there can be as many moieties as chemically permitted, and the determination of the maximum number of such moieties is well within the knowledge of those skilled in the art.
The term “pharmaceutically acceptable salt, solvate, ester or prodrug” is used throughout the specification to describe any pharmaceutically acceptable form (such as an ester, phosphate ester, salt of an ester or a related group, or hydrate) of a compound which, upon administration to a patient, provides the compound described in the specification. Pharmaceutically acceptable salts include those derived from pharmaceutically acceptable inorganic or organic bases and acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, malic acid, maleic acid, succinic acid, tartaric acid, citric acid and the like. Suitable salts include those derived from alkali metals such as potassium and sodium, alkaline earth metals such as calcium and magnesium, among numerous other acids well known in the art, for example as described herein.
The term “solvate” means a physical association of a compound of this invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. “Solvate” encompasses both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like. “Hydrate” is a solvate wherein the solvent molecule is H2O.
The term “heterocyclic” or “heterocyclyl” refers to a cyclic group that may be unsaturated, partially or fully saturated and wherein there is at least one heteroatom, such as oxygen, sulfur, nitrogen, or phosphorus in the ring. Heterocyclic or heterocyclyl groups include heteroaryl groups. Non-limiting examples of non-aromatic heterocyclyls include piperidinyl, piperazinyl, morpholinyl, pyrrolidinyl, morpholino, thiomorpholino, oxiranyl, pyrazolinyl, dioxolanyl, 1,4-dioxanyl, aziridinyl, tetrahydrofuranyl, pyrrolinyl dihydrofuranyl, dioxanyl, tetrahydropyranyl, dihydropyranyl, indolinyl, imidazolyl, tetraazacyclotetradecanyl, dioxadiazacyclododecanyl, diazepanyl, etc., wherein each of the aforementioned heterocyclyls can be unsubstituted or substituted at any substitutable position (including a heteroatom) with one or more substituents (e.g., any of those described herein, including carbonyl groups in the heterocyclic ring).
The term “heteroaryl” or “heteroaromatic”, as used herein, refers to an aromatic ring that includes at least one sulfur, oxygen, nitrogen or phosphorus in the aromatic ring. Nonlimiting examples of heteroaromatics are furanyl, pyridyl, pyrimidinyl, benzoxazolyl, 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,3,4-thiadiazole, indazolyl, 1,3,5-triazinyl, thienyl, tetrazolyl, benzofuranyl, quinolyl, isoquinolyl, benzothienyl, isobenzofuryl, indolyl, isoindolyl, benzimidazolyl, purine, carbazolyl, oxazolyl, thiazolyl, benzothiazolyl, isothiazolyl, 1,2,4-thiadiazolyl, isooxazolyl, pyrrolyl, quinazolinyl, cinnolinyl, phthalazinyl, xanthinyl, hypoxanthinyl, pyrazole, 1,2,3-triazole, 1,2,4-triazole, 1,2,3-oxadiazole, thiazine, pyridazine, benzothiophenyl, isopyrrole, thiophene, pyrazine, or pteridinyl wherein said heteroaryl or heterocyclic group can be optionally substituted with one or more substituent. In one embodiment, heterocyclyl and heteroaromatic groups include purine and pyrimidines.
Substituted aromatic or heteroaromatic rings (including aromatic or heteroaromatic portions of functional groups such as arylalkyl or heteroarylalkyl groups) can be substituted with one or more substituents. Non-limiting examples of such substituents selected from the group consisting of hydroxyl, thiol, amino, alkylamino, arylamino, alkoxy, aryloxy, nitro, cyano, sulfonic acid, halo (F, Cl, I, Br), carboxy, ester, acyl, alkyl (i.e., any of the alkyl groups described herein, such as methyl, ethyl, propyl, butyl, etc.), alkenyl (i.e., any of the alkenyl groups described herein, such as vinyl, allyl, 1-propenyl, 1-butenyl, 2-butenyl, etc.), alkynyl (i.e., any of the alkynyl groups described herein, such as 1-ethynyl, 1-propynyl, 2-propynyl, etc.), haloalkyl (i.e., any of the haloalkyl groups described herein), sulfate, sulfonate, sulfonic esters and amides, phosphoric acid, phosphonic acid, phosphate, or phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art, for example, as taught in Greene, et al., Protective Groups in Organic Synthesis, John Wiley and Sons, Second Edition, 1991.
Functional oxygen and nitrogen groups (e.g., on a aryl or heteroaryl group) can be protected as necessary or desired. Suitable protecting groups are well known to those skilled in the art, and include trimethylsilyl, dimethylhexylsilyl, t-butyldimethylsilyl, and t-butyldiphenylsilyl, trityl or substituted trityl, alkyl groups, acyl groups such as acetyl and propionyl, methanesulfonyl, and p-toluenesulfonyl.
The term “heteroarylalkyl”, as used herein, means heteroaryl-alkyl-, wherein the heteroaryl and alkyl moieties can be any heteroaryl or alkyl defined herein. Non-limiting examples of heteroarylalkyl include pyridine-methyl- and benzimidazole-methyl-.
The term “heterocyclylalkyl”, as used herein, means heterocyclyl-alkyl-, wherein the alkyl moiety may attach to the heterocyclyl ring at any available position, and the heterocyclyl and alkyl moieties can be any heterocyclyl or alkyl defined herein. Non-limiting examples of heteroarylalkyl include pyrrolidine-methyl- and piperidine-methyl.
The term “carbocyclyl” or “carbocyclic” as used herein means a cyclic group in which all of the ring atoms are carbon, which may be unsaturated, partially or fully saturated. Carbocyclic rings can be monocyclic or polycyclic and include aromatic rings (e.g., phenyl, naphthyl, etc.), partially unsaturated non-aromatic rings (dehydronaphthyl, cyclopentenyl, cyclohexenyl, etc.), or fully saturated hydrocarbon rings (cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, etc.). The carbocyclic rings can be substituted with any substituents described herein, e.g., those described for substituted aromatic and heteroaromatic rings herein (including carbonyl groups in the ring).
The term “carbocyclylacyl”, as used herein, means —C(O)-carbocyclyl, wherein the carbocyclyl moiety is any carbocyclic as defined herein, and include “arylacyl” groups.
The term “arylacyl”, as used herein, means —C(O)-aryl, wherein the aryl moiety is any aryl as defined herein.
The term “aldiminyl” as used herein means a group of the Formula —C═N—R, where R is any alkyl, carbocyclyl, or heterocyclyl groups described herein. For example, aldiminyl includes —C═N-alkyl, wherein the alkyl moiety includes any alkyl moiety described herein; —C═N-aryl wherein the aryl moiety includes any described or defined herein; —C═N-cycloalkyl, wherein the cycloalkyl moiety includes any described or defined herein; —C═N-heteroaryl wherein the heteroaryl moiety includes any described or defined herein; —C═N-heterocycloalkyl, wherein the heterocycloalkyl moiety includes any described or defined herein
The term “heteorycyclylacyl”, as used herein, means —C(O)— heteorycyclyl, wherein the heteorycyclylmoiety is any heteorycyclylas defined herein, and include “heteroarylacyl” groups.
The term “heteroarylacyl”, as used herein, means —C(O)-heteroaryl, wherein the heteroaryl moiety is any heteroaryl as defined herein.
The term purine or pyrimidine includes, but is not limited to, adenine, N6-alkylpurines, N6-acylpurines (wherein acyl is C(O)(alkyl, aryl, alkylaryl, or arylalkyl), N6-benzylpurine, N6-halopurine, N6-vinylpurine, N6-acetylenic purine, N6-acyl purine, N6-hydroxyalkyl purine, N6-thioalkyl purine, N2-alkylpurines, N2-alkyl-6-thiopurines, thymine, cytosine, 5-fluorocytosine, 5-methylcytosine, 6-azapyrimidine, including 6-azacytosine, 2- and/or 4-mercaptopyrmidine, uracil, 5-halouracil, including 5-fluorouracil, C5-alkylpyrimidines, C5-benzylpyrimidines, C5-halopyrimidines, C5-vinylpyrimidine, C5-acetylenic pyrimidine, C5-acyl pyrimidine, C5-hydroxyalkyl purine, C5-amidopyrimidine, C5-cyanopyrimidine, C5-nitropyrimidine, C5-aminopyrimidine, N2-alkylpurines, N2-alkyl-6-thiopurines, 5-azacytidinyl, 5-azauracilyl, triazolopyridinyl, imidazolopyridinyl, pyrrolopyrimidinyl, and pyrazolopyrimidinyl. Purine bases include, but are not limited to, guanine, adenine, hypoxanthine, 2,6-diaminopurine, and 6-chloropurine.
Compounds of the present invention, and salts, solvates and prodrugs thereof, may exist in their tautomeric form (for example, as an amide or imino ether). All such tautomeric forms are contemplated herein as part of the present invention.
All stereoisomers (for example, geometric isomers, optical isomers and the like) of the present compounds (including those of the salts, solvates and prodrugs of the compounds as well as the salts and solvates of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this invention. Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations. The use of the terms “salt”, “solvate” “prodrug” and the like, is intended to equally apply to the salt, solvate and prodrug of enantiomers, stereoisomers, rotamers, tautomers, racemates or prodrugs of the inventive compounds.
Polymorphic forms of the compounds of the present invention, and of the salts, solvates and/or prodrugs of the compounds of the present invention, are intended to be included in the present invention.
Chemokine Inhibitory Compounds
Compounds of Formula I
In one embodiment, the invention provides compounds of Formula (I), or pharmaceutically acceptable salts, solvates, prodrugs, tautomers, stereoisomers, and esters thereof:




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof, wherein:
R1 and R2 are each independently H, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted heteroalkyl including alkoxyalkyl, haloalkyl, CF3, halogen, hydroxy, amino, optionally substituted alkyl or dialkyl amino, optionally substituted alkoxy, hydroxyalkyl, optionally substituted aminoalkyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aralkyl, optionally substituted aryalkyl, carboxy, acyl, optionally substituted alkoxycarbonyl, or optionally substituted aminocarbonyl; or
R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl;
M is H or -G-L3-Z;
E is H, —W-J-L2-Y,




L1, L2, and L3 are each independently selected from the group consisting of a covalent bond, substituted or unsubstituted alkylene, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted carbocyclyl, and substituted or unsubstituted heterocyclyl;
X, Y and Z are independently H, NRaRb, —ORc, halogen, CF3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, acyl, carboxy, optionally substituted alkoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted alkyl- or dialkylaminocarbonyl, cyano, optionally substituted heterocyclylacyl, optionally substituted carbocyclylacyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl;
Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or
Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl;
Rc is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl,
J is selected from the group consisting of a covalent bond, alkylene, —C(O)—NRd—, —C(O)—O—, —NRd—, and —C(O)—;
Rd is selected from the group consisting of H, alkyl, and substituted or substituted arylalkyl;
Q is CRc or N;
Re is selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl;
G is selected from the group consisting of a covalent bond, alkylene, —C(O)—, —C(O)—O—, and —C(O)—NRd—;
W is selected from the group consisting of a covalent bond and a substituted or unsubstituted heterocyclyl;
R3 and R4 are each independently selected from the group consisting of H, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; or
R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl;
R5 and R6 are each independently selected from the group consisting of substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted heterocyclylalkyl, alkyl, alkenyl, and alkynyl; or
-L2-Y and R6, together with the atoms to which they are shown attached form a fused substituted or unsubstituted ring; or
E and -L1X, together with the atoms to which they are shown bonded form a substituted or unsubstituted heterocyclyl;
R7 is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted carbocyclylalkyl, and substituted or unsubstituted heterocyclylalkyl; or
L2Y and R7 together with the atoms to which they are shown bonded form a fused substituted or unsubstituted ring; and
A and B are each independently one or more substituents selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl;
with the proviso that only one of M, E and L1X is H.
In certain embodiments, the compounds of Formula I have sufficient chemical stability for Formulation in a pharmaceutical composition.
In certain embodiments of Formula I, R1 and R2 are each independently H or alkyl; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl.
In certain embodiments of Formula I, X and Z are each independently selected from the group consisting of H, NRaRb, and —ORc.
When R1 and R2, taken together with the carbon atoms to which they are shown attached form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl, the carbocyclyl groups can include saturated fused rings, such as cyclopentyl, cyclohexyl, cycloheptyl, etc. fused to a pyridyl ring of the parent moiety of Formula (I) or partially or fully unsaturated fused rings. Certain non-limiting examples of such saturated fused rings include:




and non-limiting examples of unsaturated fused rings include:




When R1 and R2 combine to form a substituted or unsubstituted heterocyclyl, the substituted or unsubstituted heterocyclyl includes one or more heteroatoms at any suitable stable position(s) in the ring. Non-limiting examples include:




wherein R is any suitable substituent (including H) described herein, and L1, X, E and M are as defined above for Formula (I). In certain embodiments, R is H. In certain other embodiments, R is a substituted alkyl, alkenyl, aralkyl, a residue of an amino acid, or the like.

In some embodiments of Formula (I), the group




is selected from:




In a particular embodiment of Formula (I), the group




In some embodiments, when X, Y, or Z is NRaRb, R, and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl. In certain embodiments, the heterocyclyl is selected from piperidyl, piperazyl, morpholinyl, pyridyl, pyrimidyl, pyrrolyl, diazinyl, triazinyl or the like.
When E is —W-J-L2-Y, non limiting examples of —W-J-L2-Y include substituted or unsubstituted heterocyclyl rings (including, as non-limiting examples, pyridyl, piperazyl, piperadyl and pyrimidyl) substituted with an aminoalkyl, carbocyclyl, heterocyclyl, or the like, any of which can be substituted or unsubstituted.
Non-limiting examples of groups formed when R3 and R4 taken together with the carbon atoms to which they are shown attached form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl include:







Each of the preceeding may be further substituted with one or more substituents.

Compounds of Formula IA

In one embodiment, the compounds of the present invention have the following Formula (IA):




wherein each of R1, R2, R3, R4, L1, L2, X, Y, Q, A, and B are as defined or described herein.

In a particular embodiment of the compounds of Formula (IA), R1 and R2 are each independently H or alkyl; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl; L1 and L2 are each independently selected from the group consisting of a covalent bond, substituted or unsubstituted alkylene, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; X and Y are each independently H or NRaRb wherein Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl; Q is CRe or N; Re is selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl; and R3 and R4 are each independently selected from the group consisting of H, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; or R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl.
In another embodiment of the compounds of Formula (IA), R2 is H; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl; L1 is substituted or unsubstituted alkylene; L2 is a covalent bond or substituted or unsubstituted alkylene; X and Y are each independently H or NRaRb; Ra and Rb are each independently selected from the group consisting of H, alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl; Q is CH or N; and R3 and R4 are each H; or R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted aryl.
In some embodiments of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In other embodiments of the compounds of Formula (IA),




In other embodiments of the compounds of Formula (IA),




In other embodiments of the compounds of Formula (IA),




In some embodiments of the compounds of Formula (IA), Q is N.
In other embodiments of the compounds of Formula (IA), Q is CH.
In certain embodiments of the compounds of Formula (IA), L2Y is H.
In other embodiments of the compounds of Formula (IA), L2Y is -alkylene-NRaRb.
In yet other embodiments of the compounds of Formula (IA), L2Y is -alkylene-NH2.
In further embodiments of the compounds of Formula (IA), L2Y is -alkylene-N(alkyl)2.
In further embodiments of the compounds of Formula (IA), L2Y is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IA), L2Y is —(CH2)4—N(alkyl)2.
In some embodiments of the compounds of Formula (IA), L1X is H.
In other embodiments of the compounds of Formula (IA), L1X is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH2.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—N(alkyl)2.
In certain embodiments of the compounds of Formula (IA),
R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In other embodiments of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In some embodiments of the compounds of Formula (IA), L1X is alkyl; and L2Y is -alkylene-NRaRb.
In other embodiments of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-NH2.
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-N(heteroarylalkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-N(arylalkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is CH3; and L2Y is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NRaRb; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-N(alkyl)2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—N(alkyl)2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—N(alkyl)2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—N(alkyl)2; and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH(alkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH(alkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH(alkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH(alkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH(heterocyclyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH(heterocyclyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH(heterocyclyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH(heterocyclyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH(heterocyclylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH(heterocyclylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH(heterocyclylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH(heterocyclylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH(heteroarylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH(heteroarylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH(heteroarylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH(heteroarylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is -alkylene-NH(arylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)2—NH(arylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)3—NH(arylalkyl); and L2Y is H.
In another embodiment of the compounds of Formula (IA), L1X is —(CH2)4—NH(arylalkyl); and L2Y is H.
In one embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is alkyl; and L2Y is -alkylene-NRaRb.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NRaRb.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH2.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH(alkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH(heterocyclyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is -alkylene-NH(arylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH2.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH(alkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)2—NH(arylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH2.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH(alkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)3—NH(arylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH2.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH(alkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is CH3; and L2Y is —(CH2)4—NH(arylalkyl).

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NRaRb; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-N(alkyl)2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH(alkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH(heterocyclyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH(heterocyclylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH(heteroarylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is -alkylene-NH(arylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—N(alkyl)2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH(alkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH(heterocyclyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH(heterocyclylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH(heteroarylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)2—NH(arylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—N(alkyl)2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH(alkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH(heterocyclyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH(heterocyclylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH(heteroarylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)3—NH(arylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—N(alkyl)2; and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH(alkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH(heterocyclyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH(heterocyclylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH(heteroarylalkyl); and L2Y is H.

In another embodiment of the compounds of Formula (IA), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




L1X is —(CH2)4—NH(arylalkyl); and L2Y is H.

In certain specific embodiments, the invention provides compounds of Formula (IA-1), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, R1, R2, L1, L2, Q, X, and Y are as defined above for Formula (I).
In one embodiment of Formula IA-1, Q is nitrogen. In another embodiment of Formula IA-1, Q is CRe.
In another embodiment of Formula IA-1,




In still another embodiment of Formula IA-1, Q is nitrogen and




In yet another embodiment of Formula IA-1,




In another embodiment of Formula IA-1, Q is nitrogen and




In another embodiment of Formula IA-1, Q is nitrogen, and R1 is hydrogen, alkyl, halogen, hydroxy, amino, alkyl or dialkyl amino, alkoxy, acyl, alkoxycarbonyl or CF3; and R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl.
In another embodiment of Formula IA-1, Q is nitrogen and R1 is hydrogen, alkyl, halogen or CF3.
In another embodiment of Formula IA-1, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.

In still another embodiment of Formula IA-1, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.

In another embodiment of Formula IA-1, Q is nitrogen,




In yet another embodiment of Formula IA-1, Q is nitrogen,




In still another embodiment of Formula IA-1, R1 is hydrogen, alkyl, halogen, alkoxy, acyl, alkoxycarbonyl or CF3; R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl; and L1-X is




In another embodiment of Formula IA-1, L2 is a bond or alkylene, and Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3.
In still another embodiment of Formula IA-1, L2 is a bond or alkylene, and Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3; and L1-X is




In certain embodiments of Formula IA-1, the compound has the stereochemical configuration of Formula IA-1S:




wherein the variables R1, R2, A, B, L1, L2, Q, X, and Y are as defined for Formula IA-1.

In another embodiment of Formula IA-1, the compound has the stereochemical configuration of Formula IA-1R:




wherein the variables R1, R2, A, B, L1, L2, Q, X, and Y are as defined for Formula IA-1.

In another embodiment, the invention provides compounds of Formula (IA-2), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, L1, L2, Q, X, and Y are as defined above for Formula (I).

In one embodiment of Formula IA-2, Q is nitrogen. In another embodiment of Formula IA-2, Q is CRe.
In another embodiment of Formula IA-2, Q is nitrogen, L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.
In another embodiment of Formula IA-2, Q is nitrogen, and L1-X is




In certain embodiments of Formula IA-2, Q is nitrogen, L2 is a bond or alkylene, and Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3.
In still another embodiment of Formula IA-2, Q is nitrogen, L1 and L2 are independently a bond or alkylene, Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen, or CF3; and X is NRaRb.
In still another embodiment of Formula IA-2, Q is nitrogen, L1 and L2 are independently a bond or alkylene, Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen, or CF3; and L1-X is




In one embodiment of Formula IA-2, the compound has the stereochemical configuration of Formula IA-2S:




wherein the variables A, B, L1, L2, Q, X, and Y are as defined for Formula IA-2.
In another embodiment of Formula IA-2, the compound has the stereochemical configuration of Formula IA-2R:




wherein the variables A, B, L1, L2, Q, X, and Y are as defined for Formula IA-2.
In another embodiment, the invention provides compounds of Formula (IA-3), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, R1, R2, L1, L2, Q, X, and Y are as defined above for Formula (I).

In one embodiment of Formula IA-3, Q is nitrogen. In another embodiment of Formula IA-3, Q is CRe.
In another embodiment of Formula IA-3,




In still another embodiment of Formula IA-3, Q is nitrogen and




In yet another embodiment of Formula IA-3,




In another embodiment of Formula IA-3, Q is nitrogen and




In another embodiment of Formula IA-3, Q is nitrogen, R1 is hydrogen, alkyl, halogen, hydroxy, amino, alkyl or dialkyl amino, alkoxy, acyl, alkoxycarbonyl or CF3; and R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl.
In another embodiment of Formula IA-3, Q is nitrogen and R1 is hydrogen, alkyl, halogen or CF3.
In another embodiment of Formula IA-3, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.

In still another embodiment of Formula IA-3, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.

In another embodiment of Formula IA-3, Q is nitrogen,




In yet another embodiment of Formula IA-3, Q is nitrogen,




In still another embodiment of Formula IA-3, R1 is hydrogen, alkyl, halogen, alkoxy, acyl, alkoxycarbonyl or CF3; R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl; and L1-X is




In another embodiment of Formula IA-3, L2 is a bond or alkylene, and Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3.
In still another embodiment of Formula IA-3, L2 is a bond or alkylene, Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3; and L1-X is




In one embodiment of Formula IA-3, the compound has the stereochemical configuration of Formula IA-3S:




wherein the variables R1, R2, A, B, L1, L2, Q, X, and Y are as defined for Formula IA-3.

In another embodiment of Formula IA-3, the compound has the stereochemical configuration of Formula IA-3R:




wherein the variables R1, R2, A, B, L1, L2, Q, X, and Y are as defined for Formula IA-3.

In another embodiment, the invention provides compounds of Formula (IA-4), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, L1, L2, Q, X, and Y are as defined above for Formula (I).

In one embodiment of Formula IA-4, Q is nitrogen. In another embodiment of Formula IA-2, Q is CRe.
In certain embodiments of Formula IA-4, Q is nitrogen, L1 is an optionally substituted alkylene group, and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl or NRaRb.
In another embodiment of Formula IA-4, Q is nitrogen, and L1-X is




In another embodiment of Formula IA-4, Q is nitrogen, L2 is a bond or alkylene, and Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3.
In still another embodiment of Formula IA-4, Q is nitrogen, L1 and L2 are independently a bond or alkylene, Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen, or CF3; and X is NRaRb.
In still another embodiment of Formula IA-4, Q is nitrogen, L1 and L2 are independently a bond or alkylene, Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl including triazolyl and tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen, or CF3; and L1-X is




In one embodiment of Formula IA-4, the compound has the stereochemical configuration of Formula IA-4S:




wherein the variables A, B, L1, L2, Q, X, and Y are as defined for Formula IA-4.

In another embodiment of Formula IA-4, the compound has the stereochemical configuration of Formula IA-4R:




wherein the variables A, B, L1, L2, Q, X, and Y are as defined for Formula IA-4.

In various embodiments of Formulae IA, IA-1, IA-1S, IA-1R, IA-2, IA-2S, IA-2R, IA-3, IA-3S, IA-3R, IA-4, IA-4S and IA-4R, -L2-Y is selected from:







In various embodiments of Formulae IA, IA-1, IA-1S, IA-1R, IA-2, IA-2S, IA-2R, IA-3, IA-3S, IA-3R, IA-4, IA-4S and IA-4R, L1-X is selected from:







Compounds of Formula 1B and 1C

In another embodiment, the compounds of the present invention have the following Formula (IB) or (IC):




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof wherein variables A, X, Q, R1, R2, R5, R6, L1, L2 are all defined as in Formula I.
In certain embodiments of the compounds of Formula (IB) or (IC), R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl; L1 is a substituted or unsubstituted alkylene; L2 a covalent bond or a substituted or unsubstituted alkylene; X is H or NRaRb; Y is H, substituted or unsubstituted carbocyclyl, or substituted or unsubstituted heterocyclyl; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; Q is N; R5 is substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, or substituted or unsubstituted heterocyclylalkyl; and R6 is substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted heterocyclylalkyl, alkyl, alkenyl, and alkynyl.
In another embodiment of the compounds of Formula (IB) or (IC), R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl; L1 is a substituted or unsubstituted alkylene; L2 a covalent bond or a substituted or unsubstituted alkylene; X is H or NRaRb; Y is H, substituted or unsubstituted carbocyclyl, or substituted or unsubstituted heterocyclyl; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; Q is CH; R5 is substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, or substituted or unsubstituted heterocyclylalkyl; and R6 is substituted or unsubstituted aminoalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted heterocyclylalkyl, alkyl, alkenyl, and alkynyl.
In another embodiment of the compounds of Formula (IB) or (IC),




In another embodiment of the compounds of Formula (IB) or (IC),




In another embodiment of the compounds of Formula (IB) or (IC),




In another embodiment of the compounds of Formula (IB) or (IC), L2Y is H.
In another embodiment of the compounds of Formula (IB) or (IC), L2Y is substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is alkyl.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is CH3.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB) or (IC), L1X is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH2.
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R5 is substituted or unsubstituted aryl.
In another embodiment of the compounds of Formula (IB), R5 is substituted or unsubstituted phenyl.
In another embodiment of the compounds of Formula (IB), R5 is substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (IB), R5 is H.
In another embodiment of the compounds of Formula (IC), R6 is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH2.
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IB), R6 is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted aryl.
In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted phenyl.
In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted arylalkyl.
In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted benzyl.
In another embodiment of the compounds of Formula (IC), R6 is alkenyl.
In another embodiment of the compounds of Formula (IC), R6 is H.
In another embodiment of the compounds of Formula (IB) or (IC),




L2Y is H; and L1X is alkyl.

In another embodiment of the compounds of Formula (IB) or (IC),




L2Y is H; and L1X is CH3.

In another embodiment of the compounds of Formula (IC), R6 is substituted or unsubstituted




Compounds of Formula 1D

In another embodiment, the compounds of the present invention have the following Formula (ID):




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof wherein variables A, X, Y, Z, G, J, L1, L2 and L3 are as defined in Formula I.
In some embodiments of the compounds of Formula (ID), L1X is H; L2 is a covalent bond or a substituted or unsubstituted alkylene; L3 is substituted or unsubstituted alkylene; Y is substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, or NRaRb; Z is NRaRb; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; J is a covalent bond, —C(O)—NRd—, or —C(O)—; Rd is selected from the group consisting of H, alkyl, and substituted or substituted arylalkyl; and G is a covalent bond or —C(O)—NRd—.
In another embodiment of the compounds of Formula (ID), L1X is H.
In another embodiment of the compounds of Formula (ID), G is a covalent bond.
In another embodiment of the compounds of Formula (ID), G is —C(O)NH—.
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH2.
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L3Z is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is substituted or unsubstituted carbocyclylalkyl.
In another embodiment of the compounds of Formula (ID), L2Y is substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH2.
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (ID), L2Y is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (ID), J is —C(O)NH—.
In another embodiment of the compounds of Formula (ID), J is a covalent bond.
In another embodiment of the compounds of Formula (ID), J is —C(O)—.
In another embodiment of the compounds of Formula (ID), L1X is H; G is a covalent bond; L3Z is -alkylene-NRaRb; J is —C(O)NH—; and L2Y is substituted or unsubstituted carbocyclylalkyl or substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (ID), L1X is H; G is —C(O)NH—; L3Z is -alkylene-NRaRb; J is a covalent bond; and L2Y is substituted or unsubstituted carbocyclylalkyl or substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (ID), L1X is H; G is a covalent bond; L3Z is -alkylene-NRaRb; J is —C(O)—; and L2Y is -alkylene-NRaRb.
Compounds of Formula 1E
In another embodiment, the compounds of the present invention have the following Formula (IE):




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof, wherein variables R1, R2, A, X, Y, L1 and L2 are defined as in Formula I.
In one embodiment of the compounds of Formula (IE), R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl; L1 is substituted or unsubstituted alkylene; L2 is a covalent bond or a substituted or unsubstituted alkylene; X is H or NRaRb; Y is a substituted or unsubstituted carbocyclyl, or a substituted or unsubstituted heterocyclyl; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; and R7 is a substituted or unsubstituted alkyl or a substituted or unsubstituted aminoalkyl.
In certain embodiments of the compounds of Formula (IE),




In another embodiment of the compounds of Formula (IE), R7 is alkyl.
In another embodiment of the compounds of Formula (IE), R7 is CH3.
In another embodiment of the compounds of Formula (IE), R7 is aminoalkyl.
In another embodiment of the compounds of Formula (IE), R7 is (CH2)2NH2.
In another embodiment of the compounds of Formula (IE), R7 is (CH2)3NH2.
In another embodiment of the compounds of Formula (IE), R7 is (CH2)4NH2.
In another embodiment of the compounds of Formula (IE), L1X is alkyl or alkylene-NRaRb.
In another embodiment of the compounds of Formula (IE), L2Y is substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, or substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (IE),




and R7 is alkyl.

In another embodiment of the compounds of Formula (IE),




R7 is alkyl; and L2Y is substituted or unsubstituted aryl.

In another embodiment of the compounds of Formula (IE),




R7 is alkyl; and L2Y is substituted or unsubstituted





Compounds of Formula 1F

In another embodiment, the compounds of the present invention have the following Formula (IF):




or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof wherein variables R1, R2, A, X, Y, J, W, L1 and L2 are defined as in Formula I.
In one embodiment of the compounds of Formula (IF), R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl; L1 is substituted or unsubstituted alkylene; L2, is a covalent bond or a substituted or unsubstituted alkylene; X is H; Y is NRaRb or a substituted or unsubstituted heterocyclyl; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl; W is a substituted or unsubstituted heterocyclyl; J is a covalent bond, alkylene, —C(O)—NRd—, —NRd—, or —C(O)—; and Rd is selected from the group consisting of H, alkyl, and substituted or substituted arylalkyl.
In certain embodiments of the compounds of Formula (IF), J is a covalent bond.
In another embodiment of the compounds of Formula (IF), J is —NH—.
In another embodiment of the compounds of Formula (IF), J is —C(O)—.
In another embodiment of the compounds of Formula (IF), J is —C(O)—NH—.
In another embodiment of the compounds of Formula (IF), L1X is substituted or unsubstituted alkyl.
In another embodiment of the compounds of Formula (IF), L1X is substituted or unsubstituted CH3.
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH2.
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (IF), L2Y is substituted or unsubstituted heterocyclylalkyl.
In another embodiment of the compounds of Formula (IF),




In another embodiment of the compounds of Formula (IF),




W is a substituted or unsubstituted heterocyclyl; L1X is substituted or unsubstituted alkyl; and L2Y is -alkylene-NRaRb.

In another embodiment of the compounds of Formula (IF), J is a covalent bond.
In another embodiment of the compounds of Formula (IF), J is NH.
In another embodiment of the compounds of Formula (IF), J is —C(O)—.
In another embodiment of the compounds of Formula (IF), J is —C(O)—NH—.
In another embodiment of the compounds of Formula (IF), W is substituted or unsubstituted substitutent selected from:




Compounds of Formula 1G

In yet another embodiment, the compounds of the present invention have the following Formula (IG):




wherein each of R1, R2, R3, R4, L1, X, Q, A, and B are as defined for Formula I.

In a particular embodiment of the compounds of Formula (IG), R1 and R2 are each independently H or alkyl; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl; L1 is selected from the group consisting of a covalent bond, substituted or unsubstituted alkylene, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; X is H or NRaRb; Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl; Q is CRe or N; Re is selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl; and R3 and R4 are each independently selected from the group consisting of H, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; or R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl.
In another embodiment of the compounds of Formula (IG), R2 is H; or R1 and R2, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl; L1 is substituted or unsubstituted alkylene; X is H or NaRb; Ra and Rb are each independently selected from the group consisting of H, alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl; Q is CH or N; and R3 and R4 are each H; or R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted aryl.
In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In another embodiment of the compounds of Formula (IG),




In another embodiment of the compounds of Formula (IG),




In another embodiment of the compounds of Formula (IG),




In another embodiment of the compounds of Formula (IG), Q is N.
In another embodiment of the compounds of Formula (IG), Q is CH.
In another embodiment of the compounds of Formula (IG), L1X is H.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH2.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NRaRb.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NRaRb.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NRaRb.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IG),
R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In another embodiment of the compounds of Formula (IG),
R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In another embodiment of the compounds of Formula (IG),
R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




In another embodiment of the compounds of Formula (IG), L1X is alkyl.
In another embodiment of the compounds of Formula (IG), L1X is CH3.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NRaRb.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH2.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—N(alkyl)2.
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH(alkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH(alkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH(alkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH(alkyl).
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH(heterocyclyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH(heterocyclyl).
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH(heterocyclylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH(heteroarylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is -alkylene-NH(arylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)2—NH(arylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)3—NH(arylalkyl).
In another embodiment of the compounds of Formula (IG), L1X is —(CH2)4—NH(arylalkyl).
In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is alkyl.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is CH3.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NRaRb.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-N(alkyl)2.
In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH(alkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH(heterocyclyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is -alkylene-NH(arylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—N(alkyl)2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH(alkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)2—NH(arylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—N(alkyl)2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH(alkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)3—NH(arylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—N(alkyl)2.

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH(alkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH(heterocyclyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH(heterocyclylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH(heteroarylalkyl).

In another embodiment of the compounds of Formula (IG), R3 and R4, taken together with the carbon atoms to which they are shown attached, form:




and L1X is —(CH2)4—NH(arylalkyl).

In one embodiment, the invention provides compounds of Formula (IG-1), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, R1, R2, L1, Q, and X are as defined above for Formula (I).
In one embodiment of Formula IG-1, Q is nitrogen. In another embodiment of Formula IA-1, Q is CRe.
In another embodiment of Formula IG-1,




In still another embodiment of Formula IG-1, Q is nitrogen and




In yet another embodiment of Formula IG-1,




In another embodiment of Formula IG-1, Q is nitrogen and




In another embodiment of Formula IG-1, Q is nitrogen, and R1 is hydrogen, alkyl, halogen, hydroxy, amino, alkyl or dialkyl amino, alkoxy, acyl, alkoxycarbonyl or CF3; and R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl.
In another embodiment of Formula IG-1, Q is nitrogen and R1 is hydrogen, alkyl, halogen or CF3.
In another embodiment of Formula IG-1, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.

In still another embodiment of Formula IG-1, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.

In another embodiment of Formula IG-1, Q is nitrogen,




In yet another embodiment of Formula IG-1, Q is nitrogen,




In still another embodiment of Formula IG-1, R1 is hydrogen, alkyl, halogen, alkoxy, acyl, alkoxycarbonyl or CF3; R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl; and L1-X is




In one embodiment of Formula IG-1, the compound has the stereochemical configuration of Formula IG-1S:




wherein the variables A, B, L1, L2, Q, and X are as defined for Formula IG-1.
In another embodiment of Formula IG-1, the compound has the stereochemical configuration of Formula IG-1R:




wherein the variables A, B, L1, Q, and X are as defined for Formula IG-1.

In another embodiment, the invention provides compounds of Formula (IA-2), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, L1, Q, and X are as defined above for Formula (I). In one embodiment of Formula IG-2, Q is nitrogen. In another embodiment of Formula IA-2, Q is CRe.
In another embodiment of Formula IG-2, Q is nitrogen, L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.
In another embodiment of Formula IG-2, Q is nitrogen, and L1-X is




In one embodiment of Formula IG-2, the compound has the stereochemical configuration of Formula IG-2S:




wherein the variables A, B, L1, Q, and X are as defined for Formula IG-2.
In another embodiment of Formula IG-2, the compound has the stereochemical configuration of Formula IG-2R:




wherein the variables A, B, L1, Q, and X Y are as defined for Formula IA-2.
In another embodiment, the invention provides compounds of Formula (IG-3), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, R1, R2, L1, Q, and X are as defined above for Formula (I).
In one embodiment of Formula IG-3, Q is nitrogen. In another embodiment of Formula IA-1, Q is CRe.
In another embodiment of Formula IG-3,




In still another embodiment of Formula IG-3, Q is nitrogen and




In yet another embodiment of Formula IG-3,




In another embodiment of Formula IG-3, Q is nitrogen and




In another embodiment of Formula IG-3, Q is nitrogen, and R1 is hydrogen, alkyl, halogen, hydroxy, amino, alkyl or dialkyl amino, alkoxy, acyl, alkoxycarbonyl or CF3; and R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl.
In another embodiment of Formula IG-3, Q is nitrogen and R1 is hydrogen, alkyl, halogen or CF3.
In another embodiment of Formula IG-3, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.

In still another embodiment of Formula IG-3, Q is nitrogen,




L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.

In another embodiment of Formula IG-3, Q is nitrogen,




In yet another embodiment of Formula IG-3, Q is nitrogen,




In still another embodiment of Formula IG-3, R1 is hydrogen, alkyl, halogen, alkoxy, acyl, alkoxycarbonyl or CF3; R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl; and L1-X is




In another embodiment, the invention provides compounds of Formula (IG-4), or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof:




wherein variables A, B, L1, Q, and X are as defined above for Formula (I). In one embodiment of Formula IG-2, Q is nitrogen. In another embodiment of Formula IA-4, Q is CRe.
In another embodiment of Formula IG-4, Q is nitrogen, L1 is an optionally substituted alkylene group, and X is heterocyclyl or NRaRb.
In another embodiment of Formula IG-4, Q is nitrogen, and L1-X is




In one embodiment of Formula IG-4, the compound has the stereochemical configuration of Formula IG-4S:




wherein the variables A, B, L1, Q, and X are as defined for Formula IG-4.
In another embodiment of Formula IG-2, the compound has the stereochemical configuration of Formula IG-4R:




wherein the variables A, B, L1, Q, and X Y are as defined for Formula IA-4.
In certain specific embodiments of the compounds of Formula (I), said compounds are selected from the group consisting of:
















































































































or a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, and/or ester thereof.

In certain other embodiments, the compounds can be selected from:













In certain embodiments, a compound that is useful in the methods of the invention can be identified based on in vitro criteria. In some embodiments, a compound can be considered to inhibit a chemokine signal, be a chemokine antagonist, or be useful in the methods of treatment described herein, if it interferes with Ca+ mobilization in vitro or in vivo by a chemokine receptor agonist at an IC50 of less than about 1000 nM. In certain embodiments, the IC50 is less than about 500 nM, 450 nM, 400 nM, 350 nM, 300 nM, 250 nM, 200 nM, 100 nM, 90 nM, 80 nM, 70 nM, 60 nM, 50 nM, 40 nM, 30 nM, 25 nM, 20 nM, 10 nM, 5 nM, 1 nM, 0.5 nM, or 0.1 nM. In particular embodiments, the IC50 is in the range of about 500 nM to about 1 nM, about 400 nM to about 5 nM, about 350 nM to about 10 nM, about 300 nM to about 25 nM, about 250 nM to about 50 nM, about 200 nM to about 50 nM, about 100 nM to about 10 nM, about 70 nM to about 1 nM, or about 50 nM to about 1 nM. In some embodiments, the receptor is a CXCR4 receptor. In specific embodiments, the chemokine agonist is a natural agonist and in specific embodiments is SDF-1. In particular embodiments, the Ca+ mobilization is measured in vitro. The Ca+ flux can, for example, be measured in cells that naturally express high levels of chemokine receptor or, alternatively, a chemokine receptor can be recombinantly expressed.
In certain other embodiments, the compound is considered to inhibit a chemokine signal, be a chemokine antagonist, or be useful in the methods of treatment described herein when it inhibits impedance caused by a chemokine agonist. In particular embodiments, the impedance is stimulated by a natural agonist and in specific embodiments is SDF-1. The chemokine receptor can be any chemokine receptor but in certain embodiments is CXCR4. In particular embodiments, the IC50 of impedance is less than about 500 nM, 450 nM, 400 nM, 350 nM, 300 nM, 250 nM, 200 nM, 100 nM, 90 nM, 80 nM, 70 nM, 60 nM, 50 nM, 40 nM, 30 nM, 25 nM, 20 nM, 10 nM, 5 nM, 1 nM, 0.5 nM, or 0.1 nM. In particular embodiments, the IC50 of impedance is in the range of about 500 nM to about 1 nM, about 400 nM to about 5 nM, about 350 nM to about 10 nM, about 300 nM to about 25 nM, about 250 nM to about 50 nM, about 200 nM to about 50 nM, about 100 nM to about 10 nM, about 70 nM to about 1 nM, or about 50 nM to about 1 nM.
In one embodiment, the compound demonstrates inhibition of a chemokine agonist response in vitro or in vivo that is greater than that of AMD3100. In specific embodiments, the potency of inhibition of the compound as measured by an IC50 is at least 2 times, at least 3 times, at least 4 times, at least 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, at least 10 times, at least 15 times, or at least 20 times that of AMD3100. In a particular embodiment, the potency of inhibition of the compound as measured by an IC50 is in the range of about 2 times to about 20 times, about 3 times to about 15 times, about 2 times to about 10 times, or about 5 times to about 10 times that of AMD3100.
In certain embodiments, the compounds exhibit an enhanced safety profile. The safety profile can be tested in vitro or in vivo, but in certain embodiments is tested in vitro. Preferably, the dose at which cytotoxicity of a compound occurs in vitro or in vivo is at least 2 times, at least 3 times, at least 4 times, at least 5 times, at least 10 times, at least 25 times, at least 50 times, at least 100 times, at least 250 times, at least 500 times, or at least 1000 times the IC50 of the compound in acting as a chemokine receptor antagonist.
In other embodiments, the compound exhibits reduced hERG binding. In particular embodiments, the compound exhibits hERG binding of less than 50% at its IC50 for inhibition of a chemokine receptor. In other embodiments, the compound exhibits hERG binding of less than 50% at a concentration that is at least 2 times, at least 3 times, at least 4 times, at least 5 times, at least 10 times, at least 25 times, at least 50 times, at least 100 times, at least 250 times, at least 500 times, or at least 1000 times its IC50.
Formulations
In cases where compounds are sufficiently basic or acidic to form stable nontoxic acid or base salts, administration of the compound as a pharmaceutically acceptable salt may be appropriate. Examples of pharmaceutically acceptable salts are organic acid addition salts formed with acids, which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate, citrate, malonate, tartarate, succinate, benzoate, ascorbate, α-ketoglutarate, and α-glycerophosphate. Suitable inorganic salts may also be formed, including, sulfate, nitrate, bicarbonate, and carbonate salts.
Pharmaceutically acceptable salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion. Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
Exemplary acid addition salts include acetates, adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides, hydrobromides, hydroiodides, 2-hydroxyethanesulfonates, lactates, maleates, methanesulfonates, 2-naphthalenesulfonates, nicotinates, nitrates, oxalates, pectinates, persulfates, 3-phenylpropionates, phosphates, picrates, pivalates, propionates, salicylates, succinates, sulfates, sulfonates (such as those mentioned herein), tartarates, thiocyanates, toluenesulfonates (also known as tosylates,) undecanoates, and the like. Additionally, acids which are generally considered suitable for the formation of pharmaceutically useful salts from basic pharmaceutical compounds are discussed, for example, by S. Berge et al, Journal of Pharmaceutical Sciences (1977) 66(1) 1-19; P. Gould, International J. of Pharmaceutics (1986) 33201-217; Anderson et al, The Practice of Medicinal Chemistry (1996), Academic Press, New York; and in The Orange Book (Food & Drug Administration, Washington, D.C. on their website). These disclosures are incorporated herein by reference thereto.
Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as benzathines, dicyclohexylamines, hydrabamines (formed with N,N-bis(dehydroabietyl)ethylenediamine), N-methyl-D-glucamines, N-methyl-D-glucamides, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quaternized with agents such as lower alkyl halides (e.g. methyl, ethyl, propyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g. decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides), aralkyl halides (e.g. benzyl and phenethyl bromides), and others.
All such acid salts and base salts are intended to be pharmaceutically acceptable salts within the scope of the invention and all acid and base salts are considered equivalent to the free forms of the corresponding compounds for purposes of the invention.
The active compound can also be provided as a prodrug, which is converted into a biologically active form in vivo. A prodrug may be converted into the parent drug by various mechanisms, including enzymatic processes and metabolic hydrolysis: T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems, Vol. 14 of A.C.S. Symposium Series (1987) Harper, N. J. (1962) in Jucker, ed. Progress in Drug Research, 4:221-294; Morozowich et al. (1977) in E. B. Roche ed. Design of Biopharmaceutical Properties through Prodrugs and Analogs, APhA (Acad. Pharm. Sci.); E. B. Roche, ed. (1977) Bioreversible Carriers in Drug in Drug Design, Theory and Application, APhA; H. Bundgaard, ed. (1985) Design of Prodrugs, Elsevier; Wang et al. (1999) Curr. Pharm. Design. 5(4):265-287; Pauletti et al. (1997) Adv. Drug. Delivery Rev. 27:235-256; Mizen et al. (1998) Pharm. Biotech. 11:345-365; Gaignault et al. (1996) Pract. Med. Chem. 671-696; M. Asghamejad (2000) in G. L. Amidon, P. I. Lee and E. M. Topp, Eds., Transport Proc. Pharm. Sys., Marcell Dekker, p. 185-218; Balant et al. (1990) Eur. J. Drug Metab. Pharmacokinet., 15(2): 143-53; Balimane and Sinko (1999) Adv. Drug Deliv. Rev., 39(1-3):183-209; Browne (1997). Clin. Neuropharm. 20(1): 1-12; Bundgaard (1979) Arch. Pharm. Chemi. 86(1): 1-39; H. Bundgaard, ed. (1985) Design of Prodrugs, New York: Elsevier; Fleisher et al. (1996) Adv. Drug Delivery Rev, 19(2): 115-130; Fleisher et al. (1985) Methods Enzymol. 112: 360-81; Farquhar D, et al. (1983) J. Pharm. Sci., 72(3): 324-325; Han, H. K. et al. (2000) AAPS Pharm Sci., 2(1): E6; Sadzuka Y. (2000) Curr. Drug Metab., 1:31-48; D. M. Lambert (2000) Eur. J. Pharm. Sci., 11 Suppl 2:S1 5-27; Wang, W. et al. (1999) Curr. Pharm. Des., 5(4):265, each of which is incorporated herein by reference in its entirety.
The active compound can also be provided as a lipid prodrug. Nonlimiting examples of U.S. patents that disclose suitable lipophilic substituents that can be covalently incorporated into the compound or in lipophilic preparations, include U.S. Pat. Nos. 5,149,794 (Sep. 22, 1992, Yatvin et al.); 5,194,654 (Mar. 16, 1993, Hostetler et al., 5,223,263 (Jun. 29, 1993, Hostetler et al.); 5,256,641 (Oct. 26, 1993, Yatvin et al.); 5,411,947 (May 2, 1995, Hostetler et al.); 5,463,092 (Oct. 31, 1995, Hostetler et al.); 5,543,389 (Aug. 6, 1996, Yatvin et al.); 5,543,390 (Aug. 6, 1996, Yatvin et al.); 5,543,391 (Aug. 6, 1996, Yatvin et al.); and 5,554,728 (Sep. 10, 1996; Basava et al.).
Preferably, the pharmaceutical preparation is in a unit dosage form. In such form, the preparation is subdivided into suitably sized unit doses containing appropriate quantities of the active compound, e.g., an effective amount to achieve the desired purpose.
The actual dosage employed may be varied depending upon the requirements of the patient and the severity of the condition being treated. Determination of the proper dosage regimen for a particular situation is within the skill of the art. For convenience, the total daily dosage may be divided and administered in portions during the day as required.
For preparing pharmaceutical compositions from the compounds described by this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets and suppositories. The powders and tablets may be comprised of from about 0.1 to about 95 percent active compound. Suitable solid carriers are known in the art, e.g. magnesium carbonate, magnesium stearate, talc, sugar or lactose. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions may be found in A. Gennaro (ed.), Remington's Pharmaceutical Sciences, 18th Edition, (1990), Mack Publishing Co., Easton, Pa.
Liquid form preparations include solutions, suspensions and emulsions. As an example may be mentioned water or water-propylene glycol solutions for parenteral injection or addition of sweeteners and opacifiers for oral solutions, suspensions and emulsions. Liquid form preparations may also include solutions for intranasal administration.
Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier, such as an inert compressed gas, e.g. nitrogen.
Also included are solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for either oral or parenteral administration. Such liquid forms include solutions, suspensions and emulsions.
The compounds of the invention may also be deliverable transdermally. The transdermal compositions can take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose.
Methods of Treatment
The compounds described herein, are particularly useful for the treatment or prevention of a disorder associated with chemokine receptor binding or activation. The methods of treatment or prevention of diseases or disorders herein by administration of a compound as described herein may alternatively be a method of use of the compounds for treatment or prevention of the diseases or disorders, or may be a method of use of the compound in the manufacture of a medicament for treatment or prevention of the disease or disorder in a host. In certain embodiments, the host is a human, an in particular, a human in need of chemokine receptor inhibition. In certain embodiments, the host in need is at risk of suffering from a disorder causing a reduction in lymphocytes or myeloid cells.
Generally, the disclosure provides compositions and methods for treating or preventing a chemokine receptor mediated pathology by administering a compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof to a host in a therapeutic amount, for example in an amount sufficient to inhibit chemokine signal transduction in a cell expressing a chemokine receptor or homologue thereof.
In certain embodiments, the compounds are useful for treating disorders associated with reduced hematopoetic stem cell mobilization. The interaction between SDF-1 and CXCR4 is important in hematopoietic stem cell homing to the bone marrow and in hematopoietic stem cell quiescence. Hematopoietic stem cells can be mobilized into all blood cell types including myeloid (monocytes and macrophages, neutrophils, basophils, eosinophils, erythrocytes, megakaryocytes/platelets, dendritic cells), and lymphoid lineages (T-cells, B-cells, NK-cells).
Because the interaction between SDF-1 and CXCR4 plays an important role in holding hematopoietic stem cells in the bone marrow, the compounds described herein that are CXCR4 antagonist compounds are capable of “mobilizing” hematopoietic stem cells into the bloodstream as peripheral blood stem cells. Peripheral blood stem cell mobilization is very important in hematopoietic stem cell transplantation (an alternative to transplantation of surgically-harvested bone marrow) and is currently performed using drugs such as G-CSF.
In some embodiments, the compounds can be useful for affecting disorders that are associated with a disorder causing a hormone fluctuation or affecting the autonomic nervous system. In particular, such disorders can affect sensations involving temperature, such as hot flashes, cause dizziness or lightheadedness, or have similar somatosensory affects.
Another embodiment provides uses of a compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof for the treatment of, or for the manufacture of a medicament for the treatment of chemokine receptor mediated pathologies including, but not limited to cancer or inflammation. Still another embodiment provides uses of a chemokine peptide antagonist for the manufacture of medicament for the prevention of tumor cell metastasis in a mammal.
In a separate embodiment, a method for treating diseases of vasculature, inflammatory and degenerative diseases is provided including administering a compound of Formula (I)-(V) to a host. In one embodiment, a compound of Formula (I)-(V) is used to stimulate the production and proliferation of stem cells and progenitor cells.
In a separate embodiment, a method for the treatment of, prevention of, or reduced severity of inflammatory disease states, neovascularization, and wound healing including administering at least one compound described herein is provided.
Vascular endothelial cells express a multitude of chemokine receptors, with CXCR4 being particularly prominent (Gupta, et al. (1998) J Biol Ghem. 273: 4282; Volin, et al. (1998) Biochem Biophys Res ommnun. 242: 46). A RT-PCR based strategy which utilized CXCR4 specific primers demonstrated that mRNA for the chemokine receptor CXCR4 is expressed not only in primary cultures and transformed type II alveolar epithelial cells (pneumocytes) but also in a number of epithelial cell lines derived from various other tissues. (Murdoch, et al. (1998) Immunology. 98(1): 36-41). Unlike with endothelial cells, CXCR4 is the only chemokine receptor expressed on epithelial cells. The receptor may have a functional role in epithelial pathology. Whether CXCR4 participates in inflammatory responses remains unclear. CXCR4 expressed on the epithelium may facilitate the recruitment of phagocytic cells to sites of inflammation by direct effects on epithelial cells. CXCR4 may also have other functional roles within the immune response or participate in wound healing or neovascularization. CXCR4 may also be involved in the pathophysiology of several acute or chronic inflammatory disease states associated with the epithelium. (Murdoch, et al. (1999) Immunology. 98(1): 36-41).
In addition, the invention is directed to methods of treating animal subjects, in particular, veterinary and human subjects, to enhance or elevate the number of progenitor cells and/or stem cells. The progenitor and/or stem cells may be harvested and used in cell transplantation. In one embodiment, bone marrow progenitor and/or stem cells are mobilized for myocardial repair. Further, the invention is directed to methods of treating animal subjects, in particular, veterinary and human patients, who are defective in white blood cell (WBC) count, or who would benefit from elevation of WBC levels using the compounds disclosed herein. Moreover, the invention is directed to methods of effecting regeneration of cardiac tissue in a subject in need of such regeneration using the disclosed compounds.
The compounds of the invention may be used for the treatment of diseases that are associated with immunosuppression such as individuals undergoing chemotherapy, radiation therapy, enhanced wound healing and burn treatment, therapy for autoimmune disease or other drug therapy (e.g. corticosteroid therapy) or combination of conventional drugs used in the treatment of autoimmune diseases and graft transplantation rejection, which causes immunosuppression; immunosuppression due to congenital deficiency in receptor function or other causes; and infectious diseases, such as parasitic diseases, including but not limited to helminth infections, such as nematodes. The method of the invention thus targets a broad spectrum of conditions for which elevation of progenitor cells and/or stem cells in a subject would be beneficial or, where harvesting of progenitor cells and/or stem cell for subsequent stem cell transplantation would be beneficial. In addition, the method of the invention targets a broad spectrum of conditions characterized by a deficiency in white blood cell count, or which would benefit from elevation of said white blood cell count.
The term “progenitor cells” refers to cells that, in response to certain stimuli, can form differentiated hematopoietic or myeloid cells. The presence of progenitor cells can be assessed by the ability of the cells in a sample to form colony-forming units of various types, including, for example, CFU-GM (colony-forming units, granulocytemacrophage); CFU-GEMM (colony-forming units, -multipotential); BFU-E (burst-forming units, erythroid); HPP-CFC (high proliferative potential colony-forming cells); or other types of differentiated colonies which can be obtained in culture using known protocols. “Stem” cells are less differentiated forms of progenitor cells. Typically, such cells are often positive for CD34. Some stem cells do not contain this marker, however. In general, CD34+ cells are present only in low levels in the blood, but are present in large numbers in bone marrow.
The compounds of the invention may be administered as sole active ingredients, as mixtures of various compounds of Formula (I)-(V), and/or in admixture with additional active ingredients that are therapeutically or nutritionally useful, such as antibiotics, vitamins, herbal extracts, anti-inflammatories, glucose, antipyretics, analgesics, granulocyte-macrophage colony stimulating factor (GM-CSF), Interleukin-I (IL-i), Interleukin-3 (IL-3), Interleukin-8 (IL-8), PIXY-32 1 (GM-CSF/IL-3 fusion protein), macrophage inflammatory protein, stem cell factor, thrombopoietin, growth related oncogene or chemotherapy and the like. In addition, the compounds of the invention may be administered in admixture with additional active ingredients that are therapeutically or nutritionally useful, such as antibiotics, vitamins, herbal extracts, anti-inflammatories, glucose, antipyretics, analgesics, and the like.
The binding of SDF-1 to CXCR4 has also been implicated in the pathogenesis of atherosclerosis (Abi-Younes et al. Circ. Res. 86, 131-138 (2000)), renal allograft rejection (Either et al. Transplantation 66, 1551-1557 (1998)), asthma and allergic airway inflammation (Yssel et al. Clinical and Experimental Aller 28, 104-109 (1998); JBC vol. 164, 59355943 (2000); Gonzalo et al. J. Immunol. 165, 499-508 (2000)), Alzheimer's disease (Xia et al. J. Neurovirology 5, 32-41 (1999)) and Arthritis (Nanici et al. J. Immunol. 164, 5010-5014 (2000)).
In one particular embodiment, a method of preventing metastasis of a malignant cell is provided that includes contacting the cells with a compound of Formula I-V, or a pharmaceutically acceptable salt, ester or prodrug thereof. In a separate embodiment, a method of treating proliferative disorders by administering a compound of Formulas (I)-(V) to a host in need of treatment is provided.
In another embodiment, the invention provides a method of reducing neovascularization, particularly VEGF-dependent neovascularization, by contacting a cell with a compound of Formula (I)-(V). The cell can be in a host animal. In a separate embodiment, a method for treating diseases of vasculature, inflammatory and degenerative diseases is provided including administering a compound of Formula (I)-(V) to a host. In one embodiment, a compound of Formula (I)-(V) is used to stimulate the production and proliferation of stem cells and progenitor cells.
The compounds can prevent or reduce the severity of diseases associated with CXCR4 acitivity, and in particular of proliferative diseases in any host. However, typically the host is a mammal and more typically is a human. In certain subembodiments the host has been diagnosed with a hyperproliferative disorder prior to administration of the compound, however in other embodiments, the host is merely considered at risk of suffering from such a disorder.
Host, including humans suffering from, or at risk for, a proliferative disorder can be treated by administering an effective amount of a pharmaceutical composition of the active compound.
Abnormal cellular proliferation, notably hyperproliferation, can occur as a result of a wide variety of factors, including genetic mutation, infection, exposure to toxins, autoimmune disorders, and benign or malignant tumor induction.
There are a number of skin disorders associated with cellular hyperproliferation. Psoriasis, for example, is a benign disease of human skin generally characterized by plaques covered by thickened scales. The disease is caused by increased proliferation of epidermal cells of unknown cause. In normal skin the time required for a cell to move from the basal layer to the upper granular layer is about five weeks. In psoriasis, this time is only 6 to 9 days, partially due to an increase in the number of proliferating cells and an increase in the proportion of cells which are dividing (G. Grove, mt. J. Dermatol. 18:111, 1979). Chronic eczema is also associated with significant hyperproliferation of the epidermis. Other diseases caused by hyperproliferation of skin cells include atopic dermatitis, lichen planus, warts, pemphigus vulgaris, actinic keratosis, basal cell carcinoma and squamous cell carcinoma.
Other hyperproliferative cell disorders include blood vessel proliferation disorders, fibrotic disorders, autoimmune disorders, graft-versus-host rejection, tumors and cancers. Blood vessel proliferative disorders include angiogenic and vasculogenic disorders. Proliferation of smooth muscle cells in the course of development of plaques in vascular tissue cause, for example, restenosis, retinopathies and atherosclerosis. The advanced lesions of atherosclerosis result from an excessive inflammatory-proliferative response to an insult to the endothelium and smooth muscle of the artery wall (Ross, R. Nature, 1993, 362:801-809) Both cell migration and cell proliferation play a role in the formation of atherosclerotic lesions.
Fibrotic disorders are often due to the abnormal formation of an extracellular matrix. Examples of fibrotic disorders include hepatic cirrhosis and mesangial proliferative cell disorders. Hepatic cirrhosis is characterized by the increase in extracellular matrix constituents resulting in the formation of a hepatic scar. Hepatic cirrhosis can cause diseases such as cirrhosis of the liver. An increased extracellular matrix resulting in a hepatic scar can also be caused by viral infection such as hepatitis. Lipocytes appear to play a major role in hepatic cirrhosis.
Mesangial disorders are brought about by abnormal proliferation of mesangial cells. Mesangial hyperproliferative cell disorders include various human renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombotic micro-angiopathy syndromes, transplant rejection, and glomerulopathies.
Another disease with a proliferative component is rheumatoid arthritis. Rheumatoid arthritis is generally considered an autoimmune disease that is thought to be associated with activity of autoreactive T cells (See, e.g., Harris, B. D., Jr. (1990) The New England Journal of Medicine, 322:1277-1289), and to be caused by autoantibodies produced against collagen and IgE.
Other disorders that can include an abnormal cellular proliferative component include Behcet's syndrome, acute respiratory distress syndrome (ARDS), ischemic heart disease, post-dialysis syndrome, leukemia, acquired immune deficiency syndrome, vasculitis, lipid histiocytosis, septic shock and inflammation in general.
Examples of proliferative disorders which can be the primary tumor that is treated, or which can be the site from which metastasis is inhibited or reduced, include but are not limited to neoplasms located in the: colon, abdomen, bone, breast, digestive system, liver, pancreas, peritoneum, endocrine glands (adrenal, parathyroid, pituitary, testicles, ovary, thymus, thyroid), eye, head and neck, nervous (central and peripheral), lymphatic system, pelvis, skin, soft tissue, spleen, thorax, and urogenital tract.
Specific types of diseases include Acute Childhood Lymphoblastic Leukemia; Acute Lymphoblastic Leukemia, Acute Lymphocytic Leukemia, Acute Myeloid Leukemia, Adrenocortical Carcinoma, Adult (Primary) Hepatocellular Cancer, Adult (Primary) Liver Cancer, Adult Acute Lymphocytic Leukemia, Adult Acute Myeloid Leukemia, Adult Hodgkin's Disease, Adult Hodgkin's Lymphorria, Adult Lymphocytic Leukemia, Adult Non-Hodgkin's Lymphoma, Adult Primary Liver Cancer, Adult Soft Tissue Sarcoma, AIDS-Related Lymphorria, AIDS-Related Malignancies, Anal Cancer, Astrocytoma, Bile Duct Cancer, Bladder Cancer, Bone Cancer, Brain Stem Glioma, Brain Tumors, Breast Cancer, Cancer of the Renal Pelvis and Ureter, Central Nervous System (Primary) Lymphoma, Central Nervous System Lymphorria, Cerebellar Astrocytoma, Cerebral Astrocytoma, Cervical Cancer, Childhood (Primary) Hepatocellular Cancer, Childhood (Primary) Liver Cancer, Childhood Acute Lymphoblastic Leukemia, Childhood Acute Myeloid Leukemia, Childhood Brain Stem Glioma, Childhood Cerebellar Astrocytoma, Childhood Cerebral Astrocytoma, Childhood Extracranial Germ Cell Tumors, Childhood Hodgkin's Disease, Childhood Hodgkin's Lymphoma, Childhood Hypothalamic and Visual Pathway Glioma, Childhood Lymphoblastic Leukemia, Childhood Medulloblastoma, Childhood Non-Hodgkin's Lymphoma, Childhood Pineal and Supratentorial Primitive Neuroectodermal Tumors, Childhood Primary Liver Cancer, Childhood Rhabdomyosarcoma, Childhood Soft Tissue Sarcoma, Childhood Visual Pathway and Hypothalamic Glioma, Chronic Lymphocytic Leukemia, Chronic Myelogenous Leukemia, Colon Cancer, Cutaneous T-Cell Lymphoma, Endocrine Pancreas Islet Cell Carcinoma. Endometrial Cancer, Ependymoma, Epithelial Cancer, Esophageal Cancer, Ewing's Sarcoma and Related Tumors, Exocrine Pancreatic Cancer, Extraeranial Germ Cell Tumor, Extragonadal Germ Cell Tumor, extrahepatic Bile Duct Cancer, Eye Cancer, Female Breast Cancer, Gaucher's Disease, Gallbladder Cancer, Gastric Cancer, Gastrointestinal Carcinoid Tumor, Gastrointestinal Tumors, Germ Cell Tumors, Gestational Trophoblastic Tumor, Hairy Cell Leukemia, Head and Neck Cancer, Hepatocellular Cancer, Hodgkin's Disease, Hodgkin's Lymphoma, Hypergammaglobulinemia, hypopharyngeal Cancer, Intestinal Cancers, Intraocular Melanoma, Islet Cell Carcinoma, Islet Cell Pancreatic Cancer, Kaposi's Sarcoma, Kidney Cancer, Laryngeal Cancer, Lip and Oral Cavity Cancer, Liver Canôer, Lung Cancer, Lympho proliferative Disorders, Macroglobulinemia, Male Breast Cancer, Malignant Mesothelioma, Malignant Thymoma, Medulloblastoma, Melanoma, Mesothelioma, Metastatic Occult Primary Squamous Neck Cancer, Metastatic Primary Squamous Neck Cancer, Metastatic Squamous Neck Cancer, Multiple Myeloma, Multiple Myeloma/Plasma Cell Neoplasm, Myelodysplastic Syndrome, Myelogenous Leukemia, Myeloid Leukemia, Myeloproliferative Disorders, Nasal Cavity and Paranasal Sinus Cancer, Nasopharyrigeal Cancer, Neuroblastoma, Non-Hodgkin's Lymphoma During Pregnancy, Nonmelanoma Skin Cancer, Non-Small Cell Lung Cancer, Occult Primary Metastatic Squamous Neck Cancer, Oropharyngeal Cancer, Osteol Malignant Fibrous Sarcoma, Osteosarcomal Malignant Fibrous Histiocytoma, Osteosarcomal Malignant Fibrous Histiocytoma of Bone, Ovarian Epithelitj Cancer, Ovarian Germ Cell Tumor, Ovarian Low Malignant Potential Tumor, Pancreatic Cancer, Paraproteinemias, Purpura, Parathyroid, Cancer, Penile Cancer, Pheochromócytoma, Pituitary Tumor, Plasma Cell Neoplasm/Multiple Myeloma, Primary Central Nervous System Lymphoma, Primary Liver Cancer, Prostate Cancer, Rectal Cancer, Renal Cell Cancer, Renal Pelvis and Ureter Cancer, Retinoblastoma, Rhabdomyosarcoma, Salivary Gland Cancer, Sarcoidosis Sarcomas, Sezary Syndrome, Skin Cancer, Small Cell Lung Cancer, Small Intestine Cancer, Soft Tissue Sarcoma, Squamous Neck Cancer, Stomach Cancer, Supratentorial Primitive Neuroectodermal and Pineal Tumors, T-Cell Lymphoma, Testicular Cancer, Thymoma, Thyroid Cancer, Transitional Cell Cancer of the Renal Pelvis and Ureter, Transitional Renal Pelvis and Ureter Cancer, Trophoblastic Tumors, Ureter and Renal Pelvis Cell Cancer, Urethial Cancer, Uterine Cancer, Uterine Sarcoma, Vaginal Cancer, Visual Pathway and Hypothalamic Glioma, Vulvar Cancer, Waldenstron's Macroglobulinemia, Wilm's Tumor, and any other hyperproliferative disease located in an organ system listed above.
Hyperplastic disorders include, but are not limited to, angiofollicular mediastinal lymph node hyperplasia, angiolymphoid hyperplasia with eosinophilia, atypical melanocytic hyperplasia, basal cell hyperplasia, benign giant lymph node hyperplasia, cementum hyperplasia, congenital adrenal hyperplasia, congenital sebaceous hyperplasia, cystic hyperplasia, cystic hyperplasia of the breast, denture hyperplasia, ductal hyperplasia, endometrial hyperplasia, fibromuscular hyperplasia, foca epithelial hyperplasia, gingival hyperplasia, inflammatory fibrous hyperplasia, inflammatory papillary hyperplasia, intravascular papillary endothelial hyperplasia, nodular hyperplasia of prostate, nodular regenerative hyperplasia, pseudoepitheliomatous hyperplasia, senile sebaceous hyperplasia, and verrucous hyperplasia; leukemia (including acute leukemia (e.g., acute lymphocytic leukemia, acute myelocytic leukemia (including myeloblastic, promyelocytic, myelomonocytic, monocytic, and erythroleukemia)) and chronic leukemia (e.g., chronic myelocytic (granulocytic) leukemia and chronic lymphocytic leukemia)), polycythemia vera, lymphomas (e.g., Hodgkin's disease and non-Hodgkin's disease), multiple myeloma, Waldenstron's macroglobulinemia, heavy chain disease, and solid tumors including, but not limited to, Sarcomas and, carcinomas such as fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma, pancreatic cancer, breast cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilm's tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, emangioblastoma, acoustic neuroma, oligodendroglioma, menangioma, melanoma, neuroblastoma, and retinoblastoma.
In a separate embodiment, a method for the treatment of, prevention of, or reduced severity of, age-related macular degeneration (ARMD) and other pathogenic states involving macular retinal pigment epithelial (RPE) cells including administering at least one compound described herein is provided.
CXCR4 plays a crucial role in ocular diseases involving the retina such as age-related macular degeneration (ARMD). The retinal pigment epithelium has a major role in the physiological renewal of photoreceptor outer segments in the provision of a transport and storage system or nutrients essential to the photoreceptor layer. The retinal pigment epithelial (RPE) cells predominantly express CXCR4 receptors. (Crane, et al. (2000) J. Immunol. 165: 4372-4278). CXCR4 receptor expression on human retinal pigment epithelial cells from the blood-retina barrier leads to chemokine secretion and migration in response to stromal cell-derived factor 1a. J. Immunol. 200; 165: 4372-4278). The level of CXCR4 mRNA expression increases upon stimulation with IL-i 3 or TNFa (Dwinell, et al. (1999) Gastroenterology. 117: 359-367). RPE cells also migrated in response to SDF-1a indicating that SDF-1a/CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier. (Crane I J, Wallace C A, McKillop-Smith S, Forrester N. CXCR4 receptor expression on human retinal pigment epithelial cells from the blood-retina barrier leads to chemokine secretion and migration in response to stromal cellderived factor 1a J. Immunol. 200; 165: 4372-4278).
Age-related macular degeneration is characterized by both primary and secondary damage of macular RPE cells. Early stages of ARMD are characterized by macular drusen, and irregular proliferation and atrophy of the RPE. The late stages of ARMD present with geographic RPE atrophy, RPE detachment and rupture, choroidal neovascularization and fibrovascular disciform scarring. Common first symptoms include metamorphopisia and/or general central vision loss resulting in reading disability and difficulties in detecting faces. Late stages of ARMD cause central scomota, which is extremely disabling if occurrence is bilateral (Bressler and Bressler (1995) Ophthalmology. 1995; 102: 1206-1211).
The compounds, or pharmaceutically acceptable salts, solvates, prodrugs, or esters thereof of the present invention described herein can be used to treat or prevent cancer, in particular the spread of cancer within an organism. Cancer is a general term for diseases in which abnormal cells divide without control. Cancer cells can invade nearby tissues and can spread through the bloodstream and lymphatic system to other parks of the body. It has been discovered that the administration of a chemokine receptor antagonist to a host, for example a mammal, inhibits or reduces the metastasis of tumor cells, in particular breast cancer and prostate cancer.
There are several main types of cancer, and the disclosed compounds or compositions can be used to treat any type of cancer. For example, carcinoma is cancer that begins in the skin or in tissues that line or cover internal organs. Sarcoma is cancer that begins in bone, cartilage, fat, muscle, blood vessels, or other connective or supportive tissue.
Leukemia is cancer that starts in blood-forming tissue such as the bone marrow, and causes large numbers of abnormal blood cells to be produced and enter the bloodstream. Lymphoma is cancer that begins in the cells of the immune system.
When normal cells lose their ability to behave as a specified, controlled and coordinated unit, a tumor is formed. A solid tumor is an abnormal mass of tissue that usually does not contain cysts or liquid areas. A single tumor may even have different populations of cells within it with differing processes that have gone awry. Solid tumors may be benign (not cancerous), or malignant (cancerous). Different types of solid tumors are named for the type of cells that form them. Examples of solid tumors are sarcomas, carcinomas, and lymphomas. Leukemias (cancers of the blood) generally do not form solid tumors. The compositions described herein can be used to reduce, inhibit, or diminish the proliferation of tumor cells, and thereby assist in reducing the size of a tumor.
Representative cancers that may treated with the disclosed compositions and methods include, but are not limited to, bladder cancer, breast cancer, colorectal cancer, endometrial cancer, head & neck cancer, leukemia, lung cancer, lymphoma, melanoma, non-small-cell lung cancer, ovarian cancer, prostate cancer, testicular cancer, uterine cancer, cervical cancer, thyroid cancer, gastric cancer, brain stem glioma, cerebellar astrocytoma, cerebral astrocytoma, ependymoma, Ewing's sarcoma family of tumors, germ cell tumor, extracranial cancer, Hodgkin's disease, leukemia, acute lymphoblastic leukemia, acute myeloid leukemia, liver cancer, medulloblastoma, neuroblastoma, brain tumors generally, non-Hodgkin's lymphoma, ostessarcoma, malignant fibrous histiocytoma of bone, retinoblastoma, rhabdomyosarcoma, soft tissue sarcomas generally, supratentorial primitive neuroectodermal and pineal tumors, visual pathway and hypothalamic glioma, Wilms' tumor, acute lymphocytic leukemia, adult acute myeloid leukemia, adult non-Hodgkin's lymphoma, chronic lymphocytic leukemia, chronic myeloid leukemia, esophageal cancer, hairy cell leukemia, kidney cancer, multiple myeloma, oral cancer, pancreatic cancer, primary central nervous system lymphoma, skin cancer, small-cell lung cancer, among others.
A tumor can be classified as malignant or benign. In both cases, there is an abnormal aggregation and proliferation of cells. In the case of a malignant tumor, these cells behave more aggressively, acquiring properties of increased invasiveness.
Ultimately, the tumor cells may even gain the ability to break away from the microscopic environment in which they originated, spread to another area of the body (with a very different environment, not normally conducive to their growth) and continue their rapid growth and division in this new location. This is called metastasis. Once malignant cells have metastasized, achieving cure is more difficult.
Benign tumors have less of a tendency to invade and are less likely to metastasize. They do divide in an uncontrolled manner, though. Depending on their location, they can be just as life threatening as malignant lesions. An example of this would be a benign tumor in the brain, which can grow and occupy space within the skull, leading to increased pressure on the brain. The compositions provided herein can be used to treat benign or malignant tumors.
In certain embodiments, the compounds described herein are useful for the treatment of HIV or AIDS in a host in need thereof.
In one embodiment, a method of treating or preventing HIV infection or reduction of symptoms associated with AIDS is provided including administering a compound of at least one of Formula (I)-(V) to a host. In certain embodiments, the compound can be provided to a host before treatment of infection with another compound. In a separate embodiment, the compound is provided to a patient that has been treated for HIV infection to reduce the likelihood of recurrence, or reduce mortality associated with AIDS related symptoms. In another embodiment, the compound is administered to a host at high risk of suffering from HIV infections.
Human and simian immunodeficiency viruses (HIV and SIV, respectively) enter cells through a fusion reaction triggered by the viral envelope glycoprotein (Env) and two cellular molecules: CD4 and a chemokine receptor, generally either CCR5 or CXCR5. (Alkhatib G, Combadiere C, Croder C, Feng Y, Kennedy P E, Murphy P M, Berger E A. CC CKR5. a RANTES, MIP-1apha, MIP-1Beta receptor as a fusion cofactor for macrophage-tropic HIV-1. Science. 1996; 272: 1955-1988).
In approximately 50% of infected individuals, CXCR4-tropic (X4-tropic) viruses emerge later in HIV infection, and their appearance correlates with a more rapid CD4 decline and a faster progression to AIDS (Connor, et al. (1997) J Exp. Med. 185: 621-628). Dualtropic isolates that are able to use both CCR5 and CXCR4 are also seen and may represent intermediates in the switch from CCR5 to CXCR4 tropism (Doranz, et al. (1996) Cell. 85: 1149-1158).
Hosts, including humans suffering from, or at risk for, HIV infection can be treated by administering an effective amount of the active compound or a pharmaceutically acceptable prodrug or salt thereof in the presence of a pharmaceutically acceptable carrier or diluent.
The administration can be prophylactically for the prevention of HIV infection or reduction of symptoms associated with AIDS. The active materials can be administered by any appropriate route, for example, orally, parenterally, intravenously, intradermally, subcutaneously, or topically, in liquid or solid form. However, the compounds are particularly suited to oral delivery.
In a separate embodiment, a method for the treatment or prevention of HIV infection or reduction of symptoms associated with AIDS by administering a compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof to a host in need of treatment is provided. The compounds of the invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof can be administered to a host in need thereof to reduce the severity of AIDS related disorders. In one embodiment of the invention, the host is a human.
In a separate embodiment, a method for the treatment of, prevention of, or reduced severity of liver disease associated with viral infections including administering at least one compound described herein is provided.
Chronic hepatitis C virus (HCV) and hepatitis B virus (HBC) infection is accompanied by inflammation and fibrosis eventually leading to cirrhosis. A study testing the expression and function of CXCR4 on liver-infiltrating lymphocytes (LIL) revealed an important role for the CXCL12/CXCR4 pathway in recruitment and retention of immune cells in the liver during chronic HCV and HBV infection (Wald, et al. (2004) European Journal of Immunology. 34(4): 1164-1174). High levels of CXCR4 and TGFβ have been detected in liver samples obtained from patients infected with HCV. (Mitra, et al. (1999) Int. J. Oncol. 14: 917-925). In vitro, TGF-β has been shown to up-regulate the expression of CXCR4 on naïve T cells and to increase their migration. The CD69/TGFβ/CXCR4 pathway may be involved in the retention of recently activated lymphocytes in the liver (Wald, et al. European Journal of Immunology. 2004; 34(4): 1164-1174).
In another embodiment, the invention provides a method of treating symptoms associated with other infections associated with chemokine receptor activation, for example, liver diseases associated with flavivirus or pestivirus infection, and in particular, HCV or HBV, by contacting a cell with a compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof. The cell can be in a host animal, in particular in a human.
The compounds can treat or prevent HIV infection, or reduce the severity of AIDS related symptoms and diseases in any host. However, typically the host is a mammal and more typically is a human. In certain embodiments the host has been diagnosed with AIDS prior to administration of the compound, however in other embodiments, the host is merely infected with HIV and asymptomatic.
An exemplary dose of the compound will be in the range from about 1 to 50 mg/kg, preferably 1 to 20 mg/kg, of body weight per day, more generally 0.1 to about 100 mg per kilogram body weight of the recipient per day. The effective dosage range of the pharmaceutically acceptable salts and prodrugs can be calculated based on the weight of the parent compound to be delivered. If the salt, ester or prodrug exhibits activity in itself, the effective dosage can be estimated as above using the weight of the salt, ester, solvate, or prodrug, or by other means known to those skilled in the art.
The amount and frequency of administration of the compounds of the invention and/or the pharmaceutically acceptable salts thereof will be regulated according to the judgment of the attending clinician considering such factors as age, condition and size of the patient as well as the condition and/or severity of the symptoms being treated. A typical recommended daily dosage regimen for oral administration can range from about 0.1 mg/day to about 2000 mg/day, in one to four divided doses.
Pharmaceutical Compositions
In one embodiment, pharmaceutical compositions including at least one compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof is provided.
The compound of the present invention, or a pharmaceutically acceptable salt, solvate, prodrug, or ester thereof is conveniently administered in unit any suitable dosage form, including but not limited to one containing 7 to 3000 mg, preferably 70 to 1400 mg of active ingredient per unit dosage form. A oral dosage of 50-1000 mg is usually convenient. Ideally the active ingredient should be administered to achieve peak plasma concentrations of the active compound of from about 1 μM to 100 mM or from 0.2 to 700 μM, or about 1.0 to 10 μM.
The concentration of active compound in the drug composition will depend on absorption, inactivation, and excretion rates of the drug as well as other factors known to those of skill in the art. It is to be noted that dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the concentration ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition. The active ingredient may be administered at once, or may be divided into a number of smaller doses to be administered at varying intervals of time.
A preferred mode of administration of the active compound is oral. Oral compositions will generally include an inert diluent or an edible carrier. They may be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches or capsules. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition.
The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring. When the dosage unit form is a capsule, it can contain, in addition to material of the above type, a liquid carrier such as a fatty oil. In addition, dosage unit forms can contain various other materials which modify the physical form of the dosage unit, for example, coatings of sugar, shellac, or other enteric agents.
The compound can be administered as a component of an elixir, suspension, syrup, wafer, chewing gum or the like. A syrup may contain, in addition to the active compounds, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors.
The compound or a pharmaceutically acceptable prodrug or salts thereof can also be mixed with other active materials that do not impair the desired action, or with materials that supplement the desired action, such as antibiotics, antifungals, anti-inflammatories, or antiviral compounds, or with additional chemotherapeutic agents. Solutions or suspensions used for parenteral, intradermal, subcutaneous, or topical application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parental preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
In a preferred embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release Formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters and polylactic acid. Methods for preparation of such Formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation. If administered intravenously, preferred carriers are physiological saline or phosphate buffered saline (PBS).
In certain embodiments, the active is provided an aqueous solution. The active can be dissolved in, for example, water, PBS, or a similar inert liquid. Similarly, when in a solid form, the active can be provided in conjunction with an inert carrier. Typically, the inert carrier makes up the remained of any given volume of liquid or solid Formulation, i.e. up to 100%. When actives are combined, the ratio of the actives can vary from about 1:10,000 to about 1:1, however the ratio is more typically from about 1:1 to about 1:50, or more typically about 1:1 to about 1:10. Ratios can be measured either on a molar basis or by weight.
Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) are also preferred as pharmaceutically acceptable carriers. These may be prepared according to methods known to those skilled in the art, for example, as described in U.S. Pat. No. 4,522,811 (which is incorporated herein by reference in its entirety). For example, liposome Formulations may be prepared by dissolving appropriate lipid(s) (such as stearoyl phosphatidyl ethanolamine, stearoyl phosphatidyl choline, arachidoyl phosphatidyl choline, and cholesterol) in an inorganic solvent that is then evaporated, leaving behind a thin film of dried lipid on the surface of the container. An aqueous solution of the active compound or its monophosphate, diphosphate, and/or triphosphate derivatives is then introduced into the container. The container is then swirled by hand to free lipid material from the sides of the container and to disperse lipid aggregates, thereby forming the liposomal suspension.
Combination and Alternation Therapy
In one embodiment, the compounds described herein are administered in combination or alternation with another active compound.
In certain embodiments, at least a second active compound is administered in combination or alternation with the first compound.
The second active compound can be a chemotherapeutic agent, for example an agent active against a primary tumor. Hosts, including humans suffering from or at risk for a proliferative disorder can be treated by administering an effective amount of a pharmaceutical composition of the active compound. In particular embodiments, the compound is useful in disorders in which hematopoietic stem cell mobilization is desired, such as upon administration of a chemotherapeutic agent. In these embodiments, it may be beneficial to administer the compound described herein in combination or alternation with a chemotherapeutic agent to reduce side effects of chemotherapy.
In certain other embodiments, the second active compound can be an antiviral, particularly an agent active against a HIV and in a particular embodiment, active against HIV-1. Hosts, including humans suffering from or at risk of contracting HIV can be treated by administering an effective amount of a pharmaceutical composition of the active compound.
In one embodiment, the active compound is a compound that is used as a chemotherapeutic. The compound provided in combination or alternation can, for example, be selected from the following list:















13-cis-Retinoic Acid
2-Amino-6-
2-CdA
2-



Mercaptopurine

Chlorodeoxyadenosine


5-fluorouracil
5-FU
6-TG
6-Thioguanine


6-Mercaptopurine
6-MP
Accutane
Actinomycin-D


Adriamycin
Adrucil
Agrylin
Ala-Cort


Aldesleukin
Alemtuzumab
Alitretinoin
Alkaban-AQ


Alkeran
All-transretinoic
Alpha interferon
Altretamine



acid


Amethopterin
Amifostine
Aminoglutethimide
Anagrelide


Anandron
Anastrozole
Arabinosylcytosine
Ara-C


Aranesp
Aredia
Arimidex
Aromasin


Arsenic trioxide
Asparaginase
ATRA
Avastin


BCG
BCNU
Bevacizumab
Bexarotene


Bicalutamide
BiCNU
Blenoxane
Bleomycin


Bortezomib
Busulfan
Busulfex
C225


Calcium Leucovorin
Campath
Camptosar
Camptothecin-11


Capecitabine
Carac
Carboplatin
Carmustine


Carmustine wafer
Casodex
CCNU
CDDP


CeeNU
Cerubidine
cetuximab
Chlorambucil


Cisplatin
Citrovorum Factor
Cladribine
Cortisone


Cosmegen
CPT-11
Cyclophosphamide
Cytadren


Cytarabine
Cytarabine
Cytosar-U
Cytoxan



liposomal


Dacarbazine
Dactinomycin
Darbepoetin alfa
Daunomycin


Daunorubicin
Daunorubicin
Daunorubicin
DaunoXome



hydrochloride
liposomal


Decadron
Delta-Cortef
Deltasone
Denileukin diftitox


DepoCyt
Dexamethasone
Dexamethasone
dexamethasone sodium




acetate
phosphate


Dexasone
Dexrazoxane
DHAD
DIC


Diodex
Docetaxel
Doxil
Doxorubicin


Doxorubicin
Droxia
DTIC
DTIC-Dome


liposomal


Duralone
Efudex
Eligard
Ellence


Eloxatin
Elspar
Emcyt
Epirubicin


Epoetin alfa
Erbitux
Erwinia L-
Estramustine




asparaginase


Ethyol
Etopophos
Etoposide
Etoposide phosphate


Eulexin
Evista
Exemestane
Fareston


Faslodex
Femara
Filgrastim
Floxuridine


Fludara
Fludarabine
Fluoroplex
Fluorouracil


Fluorouracil (cream)
Fluoxymesterone
Flutamide
Folinic Acid


FUDR
Fulvestrant
G-CSF
Gefitinib


Gemcitabine
Gemtuzumab
Gemzar
Gleevec



ozogamicin


Gliadel wafer
Glivec
GM-CSF
Goserelin


granulocyte colony
Granulocyte
Halotestin
Herceptin


stimulating factor
macrophage colony



stimulating factor


Hexadrol
Hexalen
Hexamethylmelamine
HMM


Hycamtin
Hydrea
Hydrocort Acetate
Hydrocortisone


Hydrocortisone
Hydrocortisone
Hydrocortone
Hydroxyurea


sodium phosphate
sodium succinate
phosphate


Ibritumomab
Ibritumomab
Idamycin
Idarubicin



Tiuxetan


Ifex
IFN-alpha
Ifosfamide
IL-2


IL-11
Imatinib mesylate
Imidazole
Interferon alfa




Carboxamide


Interferon Alfa-2b
Interleukin-2
Interleukin-11
Intron A (interferon


(PEG conjugate)


alfa-2b)


Iressa
Irinotecan
Isotretinoin
Kidrolase


Lanacort
L-asparaginase
LCR
Letrozole


Leucovorin
Leukeran
Leukine
Leuprolide


Leurocristine
Leustatin
Liposomal Ara-C
Liquid Pred


Lomustine
L-PAM
L-Sarcolysin
Lupron


Lupron Depot
Matulane
Maxidex
Mechlorethamine


Mechlorethamine
Medralone
Medrol
Megace


Hydrochlorine


Megestrol
Megestrol Acetate
Melphalan
Mercaptopurine


Mesna
Mesnex
Methotrexate
Methotrexate Sodium


Methylprednisolone
Meticorten
Mitomycin
Mitomycin-C


Mitoxantrone
M-Prednisol
MTC
MTX


Mustargen
Mustine
Mutamycin
Myleran


Mylocel
Mylotarg

Navelbine


Neosar
Neulasta
Neumega
Neupogen


Nilandron


Nilutamide
Nitrogen Mustard
Novaldex
Novantrone


Octreotide
Octreotide acetate
Oncospar
Oncovin


Ontak
Onxal
Oprevelkin
Orapred


Orasone
Oxaliplatin
Paclitaxel
Pamidronate


Panretin
Paraplatin
Pediapred
PEG Interferon


Pegaspargase
Pegfilgrastim
PEG-INTRON
PEG-L-asparaginase


Phenylalanine
Platinol
Platinol-AQ
Prednisolone


Mustard


Prednisone
Prelone
Procarbazine
PROCRIT


Proleukin
Prolifeprospan 20
Purinethol
Raloxifene



with Carmustine



implant


Rheumatrex
Rituxan
Rituximab
Roveron-A (interferon





α-2a)


Rubex
Rubidomycin
Sandostatin
Sandostatin LAR



hydrochloride


Sargramostim
Solu-Cortef
Solu-Medrol
STI-571


Streptozocin
Tamoxifen
Targretin
Taxol


Taxotere
Temodar
Temozolomide
Teniposide


TESPA
Thalidomide
Thalomid
TheraCys


Thioguanine
Thioguanine
Thiophosphoamide
Thioplex



Tabloid


Thiotepa
TICE
Toposar
Topotecan


Toremifene
Trastuzumab
Tretinoin
Trexall


Trisenox
TSPA
VCR
Velban


Velcade
VePesid
Vesanoid
Viadur


Vinblastine
Vinblastine Sulfate
Vincasar Pfs
Vincristine


Vinorelbine
Vinorelbine tartrate
VLB
VM-26


VP-16
Vumon
Xeloda
Zanosar


Zevalin
Zinecard
Zoladex
Zoledronic acid


Zometa









In another embodiment, the second active compound is a compound that is used as an anti-HIV agent, including but not limited to a nucleoside or nonnucleoside reverse transcriptase inhibitor, a protease inhibitor, a fusion inhibitor, cytokine and interferon. The compound provided in combination or alternation can, as a nonlimiting example, be selected from the following lists:














Brand Name
Generic Name









Agenerase
amprenavir



Combivir
lamivudine and zidovudine



Crixivan
indinavir, IDV, MK-639



Emtriva
FTC, emtricitabine



Epivir
lamivudine, 3TC



Epzicom
abacavir/lamivudine



Fortovase
saquinavir



Fuzeon
enfuvirtide, T-20



Hivid
zalcitabine, ddC, dideoxycytidine



Invirase
saquinavir mesylate, SQV



Kaletra
lopinavir and ritonavir



Lexiva
Fosamprenavir Calcium



Norvir
ritonavir, ABT-538



Rescriptor
delavirdine, DLV



Retrovir
zidovudine, AZT, azidothymidine, ZDV



Reyataz
atazanavir sulfate



Sustiva
efavirenz



Trizivir
abacavir, zidovudine, and lamivudine



Truvada
tenofovir disoproxil/emtricitabine



Videx EC
enteric coated didanosine



Videx
didanosine, ddI, dideoxyinosine



Viracept
nelfinavir mesylate, NFV



Viramune
nevirapine, BI-RG-587



Viread
tenofovir disoproxil fumarate



Zerit
stavudine, d4T



Ziagen
abacavir










Further active agents include: GW5634 (GSK), (+)Calanolide A (Sarawak Med.), Capravirine (Agouron), MIV-150 (Medivir/Chiron), TMC125 (Tibotec), RO033-4649 (Roche), TMC114 (Tibotec), Tipranavir (B-I), GW640385 (GSK % Vertex), Elvucitabine (Achillion Ph.), Alovudine (FLT) (B-I), MIV-210 (GSK/Medivir), Racivir (Pharmasset), SPD754 (Shire Pharm.), Reverset (Incyte Corp.), FP21399 (Fuji Pharm.), AMD070 (AnorMed), GW873140 (GSK), BMS-488043 (BMS), Schering C/D (417690), PRO 542 (Progenics Pharm), TAK-220 (Takeda), TNX-355 (Tanox), UK-427,857 (Pfizer).
Further active agents include: Attachment and Fusion Inhibitors (i.e. AMD070, BMS-488043, FP21399, GW873140, PRO542, Schering C, SCH 417690, TAK-220, TNX-355 and UK-427,857); Integrase Inhibitors; Maturation Inhibitors (i.e. PA457); Zinc Finger Inhibitors (i.e. azodicarbonamide (ADA)); Antisense Drugs (i.e. HGTV43 by Enzo Therapeutics, GEM92 by Hybridon); Immune Stimulators (i.e. Ampligen by Hemispherx Biopharma, IL-2 (Proleukin) by Chiron Corporation, Bay 50-4798 by Bayer Corporation, Multikine by Cel-Sci Corporation, IR103 combo); Vaccine-Like Treatment (i.e. HRG214 by Virionyx, DermaVir, VIR201 (Phase I/IIa)).
In one embodiment, the compounds of the invention are administered in combination with another active agent. The compounds can also be administered concurrently with the other active agent. In this case, the compounds can be administered in the same Formulation or in a separate Formulation. There is no requirement that the compounds be administered in the same manner. For example, the second active agent can be administered via intravenous injection while the compounds of the invention may be administered orally. In another embodiment, the compounds of the invention are administered in alternation with at least one other active compound. In a separate embodiment, the compounds of the invention are administered during treatment with an active agent, such as, for example, an agent listed above, and administration of the compounds of the invention is continued after cessation of administration of the other active compound.
The compounds of the invention can be administered prior to or after cessation of administration of another active compound. In certain cases, the compounds may be administered before beginning a course of treatment for viral infection or for secondary disease associated with HIV infections, for example. In a separate embodiment, the compounds can be administered after a course of treatment to reduce recurrence of viral infections.
In another embodiment, the active compound is a compound that is used as a chemotherapeutic. A compound provided in combination or alternation can, for example, be selected from the following list:















13-cis-Retinoic Acid
2-Amino-6-
2-CdA
2-Chlorodeoxyadenosine



Mercaptopurine


5-fluorouracil
5-FU
6-TG
6-Thioguanine


6-Mercaptopurine
6-MP
Accutane
Actinomycin-D


Adriamycin
Adrucil
Agrylin
Ala-Cort


Aldesleukin
Alemtuzumab
Alitretinoin
Alkaban-AQ


Alkeran
All-transretinoic acid
Alpha interferon
Altretamine


Amethopterin
Amifostine
Aminoglutethimide
Anagrelide


Anandron
Anastrozole
Arabinosylcytosine
Ara-C


Aranesp
Aredia
Arimidex
Aromasin


Arsenic trioxide
Asparaginase
ATRA
Avastin


BCG
BCNU
Bevacizumab
Bexarotene


Bicalutamide
BiCNU
Blenoxane
Bleomycin


Bortezomib
Busulfan
Busulfex
C225


Calcium Leucovorin
Campath
Camptosar
Camptothecin-11


Capecitabine
Carac
Carboplatin
Carmustine


Carmustine wafer
Casodex
CCNU
CDDP


CeeNU
Cerubidine
cetuximab
Chlorambucil


Cisplatin
Citrovorum Factor
Cladribine
Cortisone


Cosmegen
CPT-11
Cyclophosphamide
Cytadren


Cytarabine
Cytarabine liposomal
Cytosar-U
Cytoxan


Dacarbazine
Dactinomycin
Darbepoetin alfa
Daunomycin


Daunorubicin
Daunorubicin
Daunorubicin
DaunoXome



hydrochloride
liposomal


Decadron
Delta-Cortef
Deltasone
Denileukin diftitox


DepoCyt
Dexamethasone
Dexamethason
dexamethasone




Acetate
sodium phosphate


Dexasone
Dexrazoxane
DHAD
DIC


Diodex
Docetaxel
Doxil
Doxorubicin


Doxorubicin
Droxia
DTIC
DTIC-Dome


liposomal


Duralone
Efudex
Eligard
Ellence


Eloxatin
Elspar
Emcyt
Epirubicin


Epoetin alfa
Erbitux
Erwinia-L-
Estramustine




asparaginase


Ethyol
Etopophos
Etoposide
Etoposide phosphate


Eulexin
Evista
Exemestane
Fareston


Faslodex
Femara
Filgrastim
Floxuridine


Fludara
Fludarabine
Fluoroplex
Fluorouracil


Fluorouracil (cream)
Fluoxyrnesterone
Flutamide
Folinic Acid


FUDR
Fulvestrant
G-CSF
Gefitinib


Gemcitabine
Gemtuzumab
Gemzar
Gleevec



ozogamicin


Gliadel wafer
Glivec
GM-CSF
Goserelin


granulocyte colony
Granulocyte
Halotestin
Herceptin


stimulating factor
macrophage colony



stimulating factor


Hexadrol
Hexalen
Hexamethylmelamine
HMM


Hycamtin
Hydrea
Hydrocort Acetate
Hydrocortisone


Hydrocortisone
Hydrocortisone
Hydrocortone
Hydroxyurea


sodium phosphate
sodium succinate
phosphate


Ibritumomab
Ibritumomab
Idamycin
Idarubicin



Tiuxetan


Ifex
IFN-alpha
Ifosfamide
IL-2


IL-11
Imatinib mesylate
Imidazole
Interferon alfa




Carboxamide


Interferon Alfa-2b
Interleukin-2
Interleukin-11
Intron A (interferon


(PEG conjugate)


alfaL2b)


Iressa
Irinotecan
Isotretinoin
Kidrolase


Lanacort
L-asparaginase
LCR
Letrozole


Leucovorin
Leukeran
Leukine
Leuprolide


Leurocristine
Leustatin
Liposomal Ara-C
Liquid Pred


Lomustine
L-PAM
L-Sarcolysin
Lupron


Lupron Depot
Matulane
Maxidex
Mechlorethamine


Mechlorethamine
Medralone
Medrol
Megace


hydrochloride


Megestrol
Megestrol Acetate
Melphalan
Mercaptopurine


Mesna
Mesnex
Methotrexate
Methotrexate





Sodium


Methylprednisolone
Meticorten
Mitomycin
Mitomycin-C


Mitoxantrone
M-Prednisol
MTC
MTX


Mylocel
Mylotarg
Navelbine
Neosar


Neulasta
Neumega
Neupogen
Nilandron


Nilutamide
Nitrogen Mustard
Novaldex
Novantrone


Octreotide
Octreotide acetate
Oncospar
Oncovin


Ontak
Onxal
Oprevelkin
Orapred


Orasone
Oxaliplatin
Paclitaxel
Pamidronate


Panretin
Paraplatin
Pediapred
PEG Interferon


Pegaspargase
Pegfilgrastim
PEG-INTRON
PEG-L-asparaginase


Phenylalanine
Platinol
Platinol-AQ
Prednisolone


Mustard


Prednisone
Prelone
Procarbazine
PROCRIT


Proleukin
Prolifeprospan 20
Purinethol
Raloxifene



with Carmustine



implant


Rheumatrex
Rituxan
Rituximab
Roveron-A





(interferon α-2a)


Rubex
Rubidomycin
Sandostatin
Sandostatin LAR



hydrochloride


Sargramostim
Solu-Cortef
Solu-Medrol
STI-571


Streptozocin
Tamoxifen
Targretin
Taxol


Taxotere
Temodar
Temozolomide
Teniposide


TESPA
Thalidomide
Thalomid
TheraCys


Thioguanine
Thioguanine Tabloid
Thiophosphoamide
Thioplex


Thiotepa
TICE
Toposar
Topotecan


Toremifene
Trastuzumab
Tretinoin
Trexall


Trisenox
TSPA
VCR
Velban


Velcade
VePesid
Vesanoid
Viadur


Vinorelbine
Vinorelbine tartrate
VLB
VM-26


VP-16
Vumon
Xeloda
Zanosar


Zevalin
Zinecard
Zoladex
Zoledronic acid


Zometa









EXAMPLES
Example 1
General Preparation of Compounds of Formula (IA)
Compounds of Formula (IA):




wherein R1, R2, R3, R4, L1, L2, X, Y, Q, A, and B are as defined herein. Exemplary compounds of Formula (IA) are shown in Table 1.













TABLE 1





Com- pound
—R3
—R4
—L2—Y
Q
—L1—X
























A




H
N










 













B
H
H
H
CH










 


C
H
H
H
CH
CH3






 












D




H
N










 


E




H
N










 


F




H
N










 


G




H
N










 


H




H
N










 


I




H
N










 


J




H
N










 


K




H
N










 


L




H
N










 


M




H
N










 


N




H
N










 


O




H
N










 


P




H
N










 


Q




H
N










 


R








N
CH3






 


S








N
CH3






 


T








N
CH3






 


U








N
CH3






 


V




H
N
CH3






 


V1




H
N

















The compounds of Formula (IA) can be prepared, for example, as described below.








Example 2
Preparation of N-((9H-pyrido[3,4-b]indol-1-yl)methyl)-N-(4-aminobutyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (A)
Step a: Preparation of ethyl-9H-pyrido[3,4-b]indole-1-carboxylate (1). To a suspension of tryptamine hydrochloride (2.00 g, 10.17 mmol) in ethanol (25 mL) was added a solution of glyoxylic acid ethyl ester (3.12 g, 15.26 mmol) in toluene (50% v/v) at 0° C. After the reaction mixture was stirred overnight at ambient temperature, the solvent was removed under reduced pressure. The resulting residue was treated with saturated aqueous sodium bicarbonate. The product was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over sodium sulfate and concentrated under reduced pressure. The crude product was used in the next step without further purification.

Step b: To a solution of the resulting crude residue in o-xylene (20 mL) at 0° C. was added 10% Pd/C (0.51 g). The mixture was stirred overnight at 140° C. under an air atmosphere. The reaction mixture was filtered through Celite, washed with chloroform and concentrated. The resulting residue was purified by chromatography on silica gel (0-20% ethyl acetate/hexanes) to afford 1.26 g (52% yield) of product 1: 1H NMR (400 MHz, CDCl3) δ 9.92 (bs, 1H), 8.59 (d, J=4.8 Hz, 1H), 8.16-8.13 (m, 2H), 7.62-7.55 (m, 2H), 7.32 (dt, J=1.2, 7.0 Hz, 1H), 4.59 (q, J=7.2 Hz, 2H), 1.53 (t, J=7.2 Hz, 3H); ESI+ MS: m/z (rel intensity) 241.1 (100, [M+H]+).

Step c: Preparation of 9H-pyrido[3,4-b]indole-1-carbaldehyde (2). To a stirred solution of ethyl-9H-pyrido[3,4-b]indole-1-carboxylate, 1, (1.26 g, 5.24 mmol) in CH2Cl2 (50 mL) was added a DIBAL-H solution (1.0 M in toluene; 36.0 mL, 36.71 mmol) at −50° C. The mixture was stirred at −50° C. for 10 min and quenched by sequential addition of methanol (14.0 mL) and 10% NaOH (10 mL) at −50° C. Then the mixture was stirred at ambient temperature for an additional 1 h. The precipitates were removed by filtration through Celite and washed with CHCl3-methanol (10:1). The combined filtrates were washed with brine, dried over sodium sulfate and concentrated under reduced pressure. The product was purified by chromatography on silica gel (2-20% methanol/CH2Cl2) to afford 0.46 g (45% yield) of β-carboline-1-carbaldehyde, 2: 1H NMR (400 MHz, CDCl3) δ 10.33 (s, 1H), 10.04 (bs, 1H), 8.63 (d, J=5.2 Hz, 1H), 8.18-8.15 (m, 2H), 7.64-7.57 (m, 2H), 7.35 (dt, J=1.6, 7.6 Hz, 1H); ESI+ MS: m/z (rel intensity) 197.0 (100, [M+H]+).

Steps d and e: Preparation of tert-butyl 4-(5,6,7,8-tetrahydroquinolin-8-ylamino)butylcarbamate (3). To a solution of 6,7-dihydroquinolin-8(5H)-one (2.0 g, 13.6 mmol) in ethanol (15 mL) was added concentrated acetic acid (5 drops), tert-butyl 4-aminobutylcarbamate (2.7 g, 14.3 mmol) and 4 Å molecular sieves. The reaction mixture was heated at 150° C. in microwave reactor for 10 min. The mixture was cooled to room temperature and NaBH4 (0.8 g, 20.4 mmol) was added all at once. The crude reaction mixture was absorbed onto silica gel and the product purified by silica gel chromatography (0% to 10% methanol/CH2Cl2) to afford the desired product: ESI+ MS: m/z (rel intensity) 320.2 (100, [M+H]+)

Step f: Preparation of tert-butyl 4-(((9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)butylcarbamate (4). A heavy-walled Pyrex tube was charged with a solution of 9H-pyrido[3,4-b]indole-1-carbaldehyde, 2, (0.20 g, 1.02 mmol) in 1,2-dichloroethane (5 mL). This solution was treated with tert-butyl 4-(5,6,7,8-tetrahydroquinolin-8-ylamino)butylcarbamate, 3, (0.36 g, 1.12 mmol), sodium triacetoxyborohydride (0.43 g, 2.04 mmol), 4A molecular sieves and a catalytic amount of acetic acid (2 drops). The reaction mixture was exposed to microwave irradiation for 10 min at a temperature of 100° C. After the irradiation, the reaction mixture was cooled with high-pressure air until the temperature had fallen below 40° C. The crude reaction mixture was poured onto a saturated aqueous solution of sodium bicarbonate. The product was extracted with dichloromethane. The combined organic layers were washed with brine, dried over potassium carbonate, filtered and concentrated in vacuo. The crude product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.12 g (24% yield) of desired product 4: 1H NMR (400 MHz, CDCl3) δ 13.43 (bs, 1H), 8.67 (d, J=3.6 Hz, 1H), 8.24 (d, J=5.6 Hz, 1H), 8.10 (d, J=7.6 Hz, 1H), 7.82 (d, J=5.2 Hz, 1H), 7.69 (d, J=8.4 Hz, 1H), 7.54 (dt, J=1.2, 7.4 Hz, 1H), 7.41 (d, J=7.2 Hz, 1H), 7.21 (dt, J=0.8, 7.2 Hz, 1H), 7.14 (dd, J=4.8, 7.6 Hz, 1H), 4.28-4.02 (m, 4H), 2.90-2.60 (m, 5H), 2.44-2.26 (m, 2H), 2.12-2.04 (m, 1H), 1.96-1.84 (m, 1H), 1.80-1.65 (m, 1H), 1.44-1.25 (m, 1H), 1.33 (s, 9H), 1.22-1.12 (m, 1H); ESI+ MS: m/z (rel intensity) 500.2 (100, [M+H]+).

Step g: Preparation of N-((9H-pyrido[3,4-b]indol-1-yl)methyl)-N-(4-aminobutyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (A). A solution of tert-butyl 4-(((9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)-butylcarbamate, 4, (0.12 g, 0.24 mmol) in methanol (5 mL) was treated with thionyl chloride (1 mL) at room temperature. The resulting mixture was stirred for 30 min. The reaction mixture was then concentrated and dried under reduced pressure to afford 0.11 g (80% yield) of the desired product A: 1H NMR (400 MHz, d6-DMSO) δ 13.49 (bs, 1H), 8.80-8.41 (m, 3H), 8.00-7.82 (m, 3H), 7.80 (d, J=8.0 Hz, 1H), 7.78-7.70 (m, 1H), 7.39 (t, J=7.2 Hz, 1H), 4.95-4.85 (m, 1H), 4.68-4.50 (m, 2H), 2.98-2.82 (m, 2H), 2.61-2.50 (m, 2H), 2.47-2.35 (m, 3H), 2.12-1.94 (m, 2H), 1.80-1.30 (m, 5H); ESI+ MS: m/z (rel intensity) 400.2 (100, [M+H]+).





Example 3
Preparation of N-((9H-pyrido[3,4-b]indol-1-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (V)
A solution of β-carboline-1-carbaldehyde, 2, (0.10 g, 0.50 mmol) in methanol (10 mL) was treated with N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.09 g, 0.56 mmol). The resulting mixture was warmed to 65° C. and stirred for 18 h. The reaction mixture was then cooled to 0° C. and sodium borohydride (0.08 g, 2.03 mmol) was added portionwise. The reaction mixture was slowly warmed to room temperature and stirred for 1 h. A saturated aqueous solution of sodium bicarbonate was added. The product was extracted with dichloromethane. The combined organic layers were dried over potassium carbonate, filtered and concentrated in vacuo. The product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.07 g (40% yield) of the desired product V: 1H NMR (400 MHz, CDCl3) δ13.20 (bs, 1H), 8.73 (d, J=4.4 Hz, 1H), 8.27 (d, J=5.6 Hz, 1H), 8.12 (d, J=7.6 Hz, 1H), 7.85 (d, J=5.2 Hz, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.55 (t, J=7.6 Hz, 1H), 7.44 (d, J=8.0 Hz, 1H), 7.25-7.15 (m, 2H), 4.26-4.18 (m, 3H), 2.94-2.72 (m, 2H), 2.34-2.18 (m, 1H), 2.24 (s, 3H), 2.15-2.05 (m, 1H), 2.04-1.90 (m, 1H), 1.85-1.70 (m, 1H); ESI+ MS: m/z (rel intensity) 343.1 (90, [M+H]+).




Example 4
Preparation of Compound 1128 of Scheme 3
Step a: Preparation of 9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indole-1-carbaldehyde: 4.00 mmol of cesium carbonate was added to the solution of 2.00 mmol of carboline aldehyde in 20 ml DMF. After stirring 1 hour at room temperature 3.00 mmol of N-(chloroacetyl)morpholine was added to the reaction mixture. The reaction mixture was heated to 65° C. for 24 hours and then poured to ice-water. The precipitate was filtered off, and washed with water and dried. The product was pure enough to use for the next step (75% yield). ESI+ MS: m/z (rel intensity) 324.10 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 10.21 (s, 1H), 8.66 (d, 1H, J=4.8 Hz), 8.20 (d, 1H, J=4.4 Hz), 8.17 (d, 1H, 6.8 Hz), 7.64 (dt, 1H, J=1.2, 7.2 Hz), 7.38 (q, 2H, J=7.2 Hz), 5.81 (s, 2H), 3.92 (t, 2H, J=4.4 Hz), 3.74 (t, 4H, J=4.8 Hz), 3.61 (t, 2H, J=4.8 Hz).

Step b: Preparation of (S)-1-morpholino-2-(1-((5,6,7,8-tetrahydroquinolin-8-ylamino)methyl)-9H-pyrido[3,4-b]indol-9-yl)ethanone. 1.5 mmol of 9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indole-1-carbaldehyde, 1.5 mmol of (S)-5,6,7,8-tetrahydroquinolin-8-amine, and 3.00 mmol of sodium triacetoxyborohydride in 20 ml dichloromethane were stirred for 2 hours at room temperature, then reaction was quenched with saturated sodium bicarbonate solution. Organic layer was dried over magnesium sulfate, filtered off, and evaporated. Desired product was purified with column chromatography using dichloromethane:methanol:NH4OH (9:1:0.1) solvent system (85% yield). ESI+ MS: m/z (rel intensity) 456.2 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 8.38 (d, 1H, J=4.8 Hz), 8.36 (d, 1H, J=1.6 Hz), 8.12 (d, 1H, J=7.6 Hz), 7.92 (d, 1H, J=5.6 Hz), 7.55 (dt, 1H, J=1.2, 8.0 Hz), 7.37 (d, 1H, J=7.6 Hz), 7.29 (q, 2H, J=4.8 Hz), 7.07 (q, 1H, 4.8 Hz), 6.11 (d, 1H, J=18.0 Hz), 5.74 (d, 1H, J=17.6 Hz), 4.46 (d, 1H, J=12.0 Hz), 4.33 (d, 1H, J=12.0 Hz), 4.00 (t, 1H, J=5.6 Hz), 3.82-3.76 (m, 1H), 3.73-3.60 (m, 6H), 3.55-3.50 (m, 1H), 2.78 (t, 2H, J=6.4 Hz), 2.18-2.08 (m, 1H), 1.96-1.66 (m, 4H).

Step c: Preparation of (S)-4-(((9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)-(4-(Bis-N-tertbutoxycarbonyl)amino) butylcarbamate. 1.00 mmol of (S)-1-morpholino-2-(1-((5,6,7,8-tetrahydroquinolin-8-ylamino)methyl)9H-pyrido[3,4-b]indol-9-yl)ethanone, 1.00 mmol of Bis-1-(N-tertbutoxycarbonyl)-4-oxobutylcarbamate, and 2.00 mmol of sodium triacetoxyborohydride in 20 ml 1,2-dichloroethane were stirred at room temperature for 2 hours. The reaction was quenched with saturated sodium bicarbonate solution. Organic layer was dried over magnesium sulfate, filtered off, and evaporated. The desired product was purified with column chromatography using dichloromethane:methanol:NH4OH (9:1:0.1) in 50% dichloromethane (95% yield). ESI+ MS: m/z (rel intensity) 727.3 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 8.29 (d, 1H, J=5.2 Hz), 8.21 (d, 1H, J=4.4 Hz), 8.09 (d, 1H, J=7.6 Hz), 7.86 (d, 1H, J=4.8 Hz), 7.56 (dt, 1H, J=1.2, 8.0 Hz), 7.30-7.23 (m, 3H), 7.17 (d, 1H, 18.4 Hz), 6.88 (dt, 1H, J=3.2, 4.4 Hz), 5.80 (d, 1H, J=17.2 Hz), 4.36 (s, 2H), 4.13 (q, 1H, J=6.8 Hz), 3.97 (d, 1H, J=12.8 Hz), 3.89 (d, 1H, J=14.4 Hz), 3.81-3.77 (m, 1H), 3.67-3.56 (m, 4H), 3.46-3.33 (m, 2H), 3.11-3.01 (m, 2H), 2.79-2.65 (m, 1H), 2.64-2.60 (m, 2H), 2.48-2.31 (m, 3H), 2.10-2.04 (m, 2H), 1.93-1.86 (m, 1H), 1.68-1.55 (m, 2H), 1.50 (s, 9H), 1.37 (s, 9H).

Step d: Preparation of (S)-2-(1-(((4-aminobutyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)-9H-pyrido[3,4-b]indol-9-yl)-1-morpholinoethanone. 0.95 mmol of (S)-4-(((9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)-(4-(Bis-N-tertbutoxycarbonyl)amino)butylcarbamate was dissolved in 2 ml of dichloromethane and treated with 2 ml of trifluoroacetic acid. After the reaction was stirred at room temperature for 2 hours it was cooled to 0° C. with ice bath and then neutralized with 1M NaOH solution carefully. The mixture was warmed to ambient temperature. The organic layer was separated, dried over magnesium sulfate, filtered off, and evaporated. The desired product was purified with column chromatography using dichloromethane:methanol:NH4OH (9:1:0.1). ESI+ MS: m/z (rel intensity) 527.2 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 8.31 (d, 1H, J=5.2 Hz), 8.25 (d, 1H, J=4.0 Hz), 8.11 (d, 1H, J=8.0 Hz), 7.89 (d, 1H, J=4.8 Hz), 7.57 (dt, 1H, J=1.2, 8.0 Hz), 7.31-7.25 (m, 3H), 7.18 (d, 1H, 19.6 Hz), 6.90 (dt, 1H, J=2.8, 4.8 Hz), 5.76 (d, 1H, J=17.2 Hz), 4.36 (s, 2H), 4.12 (t, 1H, J=9.6 Hz), 3.97 (d, 1H, J=12.8 Hz), 3.89 (d, 1H, J=13.6 Hz), 3.79 (td, 1H, J=3.6, 12.0 Hz), 3.70-3.55 (m, 3H), 3.45-3.40 (m, 1H), 3.35-3.30 (m, 1H), 2.82-2.76 (m, 1H), 2.68-2.59 (m, 2H), 2.42-2.32 (m, 2H), 2.21-2.17 (m, 2H), 2.10-2.04 (m, 2H), 1.63-1.59 (m, 1H), 1.25 (s, 2H), 0.97-0.76 (m, 4H). Elemental Analysis (C/H/N): C31H38N6O2×1.25 mol H2O; Calculated: 67.80/7.43/15.30; Found: 67.65/7.12/15.21

Example 5
Preparation of Compound 1162 of Scheme 3
Step e: Preparation of (S)-tert-butyl 4-(3-(((9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)propyl)piperazine-1-carboxylate. 1.00 mmol of S)-1-morpholino-2-(1-((5,6,7,8-tetrahydroquinolin-8-ylamino)methyl)9H-pyrido[3,4-b]indol-9-yl)ethanone, 1.2 mmol tert-butyl-4-(3-bromopropyl)piperazine-1-carboxylate, and 2.5 mmol triethyl amine in 10 ml acetonitrile were heated to reflux for overnight. The reaction was cooled to room temperature and poured to saturated sodium bicarbonate solution. The mixture was extracted with ethyl acetate. Organic layer was dried over magnesium sulfate, filtered and evaporated. Desired product was purified with column chromatography using dichloromethane:methanol:NH4OH (9:1:0.1) (46.88% yield). ESI+ MS: m/z (rel intensity) 682.4 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 8.32 (d, 1H, J=4.4 Hz), 8.12 (d, 1H, J=7.6 Hz), 7.91 (d, 1H, J=4.8 Hz), 7.57 (t, 1H, J=8.0 Hz), 7.38 (d, 1H, J=17.6 Hz), 7.32-7.27 (m, 3H), 6.97 (dt, 1H, J=3.2, 4.4 Hz), 5.62 (d, 1H, J=17.6 Hz), 4.36 (s, 2H), 4.14 (t, 1H, J=8.8 Hz), 4.01 (d, 1H, J=14.4 Hz), 3.93 (d, 1H, J=14.0 Hz), 3.81-3.51 (m, 6H), 3.46-3.20 (m, 4H), 3.08-2.96 (m, 2H), 2.88-2.58 (m, 4H), 2.41-2.32 (m, 2H), 2.11-2.02 (m, 2H), 1.90-1.81 (m, 2H), 1.75-1.59 (m, 6H), 1.40 (s, 9H).

Step f: Preparation of (S)-1-morpholino-2-(1-(((3-(piperazin-1-yl)propyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)9H-pyrido[3,4-b]indol-9-yl)ethanone. 0.50 mmol of (S)-tert-butyl 4-(3-(((9-(2-morpholino-2-oxoethyl)-9H-pyrido[3,4-b]indol-1-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)propyl)piperazine-1-carboxylate was dissolved in 2 ml of dichloromethane and treated with 2 ml of trifluoroacetic acid. After the reaction was stirred at ambient temperature for 2 hours it was cooled to 0° C. with ice bath and then neutralized with 1M NaOH solution carefully. Let the mixture warmed up to room temperature. The organic layer was separated, dried over magnesium sulfate, filtered off, and evaporated. The desired product was purified with column chromatography using dichloromethane:methanol:NH4OH (9:1:0.1). ESI+ MS: m/z (rel intensity) 582.3 (100, [M+H]+). 1H-NMR (400 MHz, CDCl3): δ 8.31 (d, 1H, J=5.6 Hz), 8.28 (d, 1H, J=4.8 Hz), 8.11 (d, 1H, J=9.2 Hz), 7.89 (d, 1H, J=5.2 Hz), 7.57 (t, 1H, J=6.8 Hz), 7.32-7.25 (m, 5H), 6.94 (dt, 1H, J=2.8, 4.8 Hz), 5.67 (d, 1H, J=16.4 Hz), 4.37 (s, 2H), 4.14 (t, 1H, J=9.2 Hz), 4.05 (d, 1H, J=16.8 Hz), 3.93 (d, 1H, J=14.8 Hz), 3.83-3.76 (m, 1H), 3.72-3.47 (m, 4H), 3.45-3.30 (m, 1H), 3.29-3.26 (m, 1H), 2.88-2.51 (m, 6H), 2.41-2.32 (m, 2H), 2.11-2.02 (m, 2H), 1.90-1.59 (m, 8H), 1.02-0.96 (m, 1H), 0.95-0.80 (m, 1H). Elemental Analysis (C/H/N): C34H43N7O2×0.95 mol H2O; Calculated: 68.19/7.56/16.37; Found: 68.18/7.38/16.23.





Example 6
Preparation of N1-((1H-indol-7-yl)methyl)-N1-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine (B)
Step a: Preparation of tert-butyl 4-(((1H-indol-7-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)butylcarbamate (6). A heavy-walled Pyrex tube was charged with a solution of indole-7-carboxaldehyde (0.50 g, 3.44 mmol) in 1,2-dichloroethane (5 mL). This solution was treated with tert-butyl 4-(5,6,7,8-tetrahydroquinolin-8-ylamino)butylcarbamate, 3, (1.21 g, 3.78 mmol), sodium triacetoxyborohydride (1.46 g, 6.88 mmol) and a catalytic amount of acetic acid (2 drops). The reaction mixture was exposed to microwave irradiation for 10 min at a temperature of 100° C. After the irradiation, the reaction tube was cooled with high-pressure air until the temperature had fallen below 40° C. The crude reaction mixture was poured onto a saturated aqueous solution of sodium bicarbonate. The product was extracted with dichloromethane. The combined organic layers were washed with brine, dried over potassium carbonate, filtered and concentrated in vacuo. The crude product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.54 g (35% yield) of the desired product 6: 1H NMR (400 MHz, CDCl3) δ 12.87 (bs, 1H), 8.56 (d, J=3.6 Hz, 1H), 7.53 (d, J=7.6 Hz, 1H), 7.41-7.37 (m, 2H), 7.10 (dd, J=5.2, 7.6 Hz, 1H), 6.97-6.88 (m, 2H), 6.51-6.49 (m, 1H), 4.26-4.20 (m, 2H), 3.96 (d, J=13.2 Hz, 1H), 3.60 (d, J=13.2 Hz, 1H), 2.92-2.72 (m, 3H), 2.65-2.55 (m, 2H), 2.50-2.40 (m, 1H), 2.29-2.20 (m, 1H), 2.14-2.04 (m, 1H), 1.95-1.68 (m, 2H), 1.47-1.15 (m, 2H), 1.39 (s, 9H); ESI+ MS: m/z (rel intensity) 449.2 (100, [M+H]+)

Step b: Preparation of N1-((1H-indol-7-yl)methyl)-N1-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine (B). A solution of tert-butyl 4-(((1H-indol-7-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)butylcarbamate, 6, (0.27 g, 0.60 mmol) in methanol (5 mL) was treated with thionyl chloride (1 mL) at room temperature. The resulting mixture was stirred for 30 min. The reaction mixture was then concentrated and dried under reduced pressure to afford 0.26 g (87% yield) of the desired product, B: 1H NMR (400 MHz, d6-DMSO) δ 11.97 (bs, 1H), 8.44 (d, J=4.8 Hz, 1H), 7.59 (d, J=7.2 Hz, 1H), 7.53 (d, J=7.2 Hz, 1H), 7.48-7.40 (m, 1H), 7.35-7.20 (m, 2H), 6.98 (t, J=6.8 Hz, 1H), 6.44 (bs, 1H), 4.90-4.78 (m, 2H), 3.60-3.53 (m, 2H), 3.10-3.00 (m, 2H), 2.80-2.60 (m, 4H), 2.50-2.39 (m, 1H), 2.30-1.40 (m, 7H); ESI+ MS: m/z (rel intensity) 349.2 (100, [M+H]+).





Example 7
Preparation of N-((1H-indol-7-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine (C)
A solution of indole-7-carboxaldehyde (0.48 g, 3.36 mmol) in 1,2-dichloroethane (10 mL) was treated with N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.60 g, 3.70 mmol), sodium triacetoxyborohydride (1.07 g, 5.05 mmol) and a catalytic amount of acetic acid (2 drops). The resulting mixture was warmed to 65° C. and stirred for 2 h. The reaction mixture was cooled to room temperature and a saturated aqueous solution of sodium bicarbonate was added. The product was extracted with dichloromethane. The combined organic layers were dried over potassium carbonate, filtered and concentrated in vacuo. The product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.75 g (76% yield) of the desired product: 1H NMR (400 MHz, CDCl3) δ 12.69 (bs, 1H), 8.61 (d, J=4.4 Hz, 1H), 7.85-7.81 (m, 1H), 7.68-7.65 (m, 1H), 7.59 (d, J=7.2 Hz, 1H), 7.44-7.38 (m, 2H), 7.12 (dd, J=4.4, 7.2 Hz, 1H), 7.03-6.96 (m, 2H), 6.55 (t, J=2.0 Hz, 1H), 4.22-4.17 (m, 1H), 4.00 (d, J=13.4 Hz, 1H), 3.84 (t, J=6.8 Hz, 1H), 3.73 (d, J=13.4 Hz, 1H), 3.42 (t, J=6.8 Hz, 1H), 2.92-2.72 (m, 2H), 2.28 (s, 3H), 2.14-2.04 (m, 1H), 2.00-1.88 (m, 1H), 1.85-1.70 (m, 1H); ESI+ MS: m/z (rel intensity) 292.1 (90, [M+H]+).
The skilled artisan will recognize that other compounds described in Table 1 can be prepared in a similar manner to the procedures described above, for example by substituting the appropriately substituted tetrahydroquinoline for compound 3.
Example 8
General Preparation of Compounds of Formulae (IB) and (IC)
Compounds of Formula (I), can have the following general structures (IB) and (IC):




wherein R1, R2, R5, R6, L1, L2, X, Y, Q, A, and B are as defined herein. Specific embodiments of the compounds of Formula (IB) and (IC) are shown in Table 2, below. For each of the compounds of Table 2, Q is N.










TABLE 2







Com-





pound
—R5
—R6
—L2—Y





W
—




H


 


X




—
H


 


Y
—




H


 


Z
—




H


 


AA




—
H


 


AB
—




H


 


AC
—




H


 


AD
—




H


 


AE
—




H


 


AF
—




H


 


AG
—




H


 


AH
—




H


 


AI
—




H


 


AJ
—




H


 


AK




—
H


 


AL
—




H


 


AM
—




H


 


AN
—




H


 


AO
—




H


 


AP
—




H


 


AQ
—




H


 


AR
—




H


 


AS
—




H


 


AT
—
H






 


AU
—
H






 


AV
—
H






 


AW
—
H






 


AX
—
H






 


AX1
—
H






 


AX2
—
H






 


AX3
—
H






 


AX4
—
H






 


AX5
—










 


AX6
—



















        Com- pound
          —L1—X











W
CH3







 



X
CH3







 



Y
CH3







 



Z











 



AA











 



AB











 



AC











 



AD
CH3







 



AE
CH3







 



AF
CH3







 



AG
CH3







 



AH
CH3







 



AI
CH3







 



AJ
CH3







 



AK
CH3







 



AL
CH3







 



AM
CH3







 



AN
CH3







 



AO
CH3







 



AP
CH3







 



AQ
CH3







 



AR











 



AS











 



AT
CH3







 



AU
CH3







 



AV
CH3







 



AW
CH3







 



AX
CH3







 



AX1
CH3







 



AX2
CH3







 



AX3
CH3







 



AX4
CH3







 



AX5
CH3







 



AX6
CH3


















Example 9
Preparation of N-((1H-benzo[d]imidazol-2-yl)methyl)-N-(4-aminobutyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (Y)
Step a: Preparation of (1-phenyl-1H-1,2,3-triazol-4-yl)methanol (7). Phenyl azide (2.02 g, 17.0 mmol) was dissolved in neat propargyl alcohol (3 mL) and the mixture was heated to 90° C. for 2 h. The reaction mixture was cooled to room temperature and the reaction mixture was diluted into diethyl ether and left overnight during which time 7 precipitated as a crystalline solid to afford 1.03 g (35%) of the desired product 7 while the mother liquor contained primarily the regioisomer 9: 1H NMR (400 MHz, CDCl3) δ 7.97, (s, 1H), 7.70, (d, J=8.40 Hz, 2H), 7.50, (m, 2H), 7.41 (m, 1H), 4.88, (d, J=6.00 Hz, 2H), 2.70, (bs, 1H).

Step b: Preparation of 1-phenyl-1H-1,2,3-triazole-4-carbaldehyde (9). To a solution of (1-phenyl-1H-1,2,3-triazol-4-yl)methanol, 7, (1.03 g, 5.87 mmol) in CH2Cl2 (50 mL) was added MnO2 (2.05 g, 23.5 mmol). The reaction mixture was stirred for 3 days at room temperature. The reaction mixture was then filtered through Celite© and the resulting filtrate was concentrated in vacuo. The crude material was purified by silica gel chromatography (0-5% methanol/dichloromethane) yielding 0.83 g (82%) of 9: 1H NMR (400 MHz, CDCl3) δ 10.22 (s, 1H), 8.51 (s, 1H), 7.53, (d, J=9.6 Hz, 2H), 7.58-7.49, (m, 3H).

Step c: Preparation of N-methyl-N-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (Y). To a solution of 1-phenyl-1H-1,2,3-triazole-4-carbaldehyde, 9, (0.23 g, 1.35 mmol) in 1,2-dichloroethane (2 mL) was added N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.20 g, 1.23 mmol), sodium triacetoxyborohydride (0.10 g, 1.60 mmol), and 3 drops of acetic acid. The reaction mixture was stirred for 16 h at room temperature. The reaction mixture was then poured into aqueous saturated NaCl (10 mL) and was extracted with ethyl acetate (10 mL). The organic phase was dried over MgSO4, filtered, and concentrated in vacuo. The crude material was purified by silica gel chromatography (0-5% methanol/dichloromethane) yielding 35 mg (9%) of Y: 1H NMR (400 MHz, CDCl3) δ 8.74 (s, 1H), 8.47 (s, 1H), 7.78, (d, J=8.4 Hz, 2H), 7.50-7.39, (m, 4H), 7.20, (m, 1H), 4.72, (d, J=13.6 Hz, 1H), 4.63-4.58 (m, 2H), 2.90-2.60 (m, 6H), 2.22-2.17 (m, 1H), 2.10-1.95, (m, 1H), 1.90-1.78, (m, 1H).





Example 10
Preparation of N-(4-aminobutyl)-N-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydro-quinolin-8-amine tetrahydrochloride (Z)
Step a: Preparation of tert-butyl 4-(((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)butylcarbamate (10). To a solution of 1-phenyl-1H-1,2,3-triazole-4-carbaldehyde, 9, (0.54 g, 3.10 mmol) in 1,2-dichloroethane (15 mL) was added tert-butyl 4-(5,6,7,8-tetrahydroquinolin-8-ylamino)butylcarbamate, 3, (1.10 g, 3.40 mmol), sodium triacetoxyborohydride (1.00 g, 4.70 mmol), and 3 drops of acetic acid. The reaction mixture was stirred for 4 h at 65° C. The reaction mixture was then cooled to room temperature and poured into saturated aqueous NaHCO3. The product was extracted with CH2Cl2 (3×10 mL) and the combined organic phases were dried over K2CO3, filtered, and concentrated in vacuo. The crude material was purified by silica gel chromatography (0-10% methanol/dichloromethane) yielding 850 mg (57%) of 13: 1H NMR (400 MHz, CDCl3) δ 8.74 (d, J=4.0 Hz, 1H), 8.11 (s, 1H), 7.73, (d, J=8.0 Hz, 2H), 7.52-7.46, (m, 2H), 7.39, (d, J=7.6 Hz, 1H), 7.31, (d, J=8.0 Hz, 1H), 7.01, (dd, J=7.6, 4.4 Hz, 1H), 4.08, (m, 1H), 3.93, (d, J=14.8 Hz, 1H), 3.83, (d, J=14.8 Hz, 1H), 3.10-3.00 (m, 2H), 2.85-2.61 (m, 4H), 2.15-1.95 (m, 2H), 1.93-1.60, (m, 3H), 1.55-1.35, (m, 12H); ESI+ MS: m/z (rel intensity) 477 (100, M+H).

Step b: Preparation of N-(4-aminobutyl)-N-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydro-quinolin-8-amine tetrahydrochloride (Z). To a solution of tert-butyl 4-(((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)-amino)butylcarbamate, 10 (0.85 g, 1.80 mmol) in methanol (20 mL) was added thionyl chloride (1.27 g, 10.7 mmol) at room temperature. The reaction mixture was stirred for 2 h. The reaction mixture was concentrated in vacuo yielding 790 mg (85%) of Z: 1H NMR (400 MHz, d6-DMSO) δ 10.78, (bs, 1H), 8.93, (s, 1H), 8.51 (d, J=4.4 Hz, 1H), 8.25-8.06 (m, 3H), 7.84, (d, J=7.6 Hz, 2H), 7.71, (d, J=7.2 Hz, 2H), 7.62-7.55 (m, 2H), 7.52-7.46, (m, 1H), 7.40-7.35, (m, 1H), 4.77, (m, 1H), 4.50-4.38, (m, 2H), 3.32-3.22, (m, 1H), 3.15-3.02 (m, 1H), 2.88-2.66 (m, 4H), 2.50-2.39 (m, 1H), 2.15-2.00, (m, 2H), 1.95-1.68, (m, 3H), 1.64-1.50, (m, 2H), ESI+ MS: m/z (rel intensity) 377 (100, M+H).





Example 11
Preparation of N-(4-aminobutyl)-N-((3-phenyl-3H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (AA)
Compound AA was prepared using the procedure for compound Z, except that the aldehyde corresponding to compound 8 was used in place of compound 9. 1H NMR (400 MHz, d6-DMSO) δ 8.54 (d, J=4.8 Hz, 1H), 8.48 (s, 1H), 8.24-8.10 (m, 3H), 8.07 (d, J=7.6 Hz, 1H), 7.67-7.63 (m, 1H), 7.60-7.52 (m, 3H), 4.48-4.39 (m, 1H), 4.13 (d, J=15.2 Hz, 1H), 3.95 (d, J=15.2 Hz, 1H), 2.90-2.50 (m, 7H), 2.00-1.40 (m, 7H); ESI+ MS: m/z (rel intensity) 377.2 (90, [M+H]+)




Example 12
Preparation of N1-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-N4-(pyridin-2-ylmethyl)-N1-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine hydrochloride (AB)
A solution of N-(4-aminobutyl)-N-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydro-quinolin-8-amine tetrahydrochloride, Z, (0.20 g, 0.38 mmol) in 1,2-dichloroethane (10 mL) was treated with Hünigs base (0.08 mL, 0.42 mmol), 2-pyridine carboxaldehyde (0.04 mL, 0.42 mmol), sodium triacetoxyborohydride (0.12 g, 0.57 mmol) and a catalytic amount of acetic acid (2 drops). The resulting mixture was warmed to 65° C. and stirred for 18 h. The reaction mixture was cooled to room temperature. A saturated aqueous solution of sodium bicarbonate was added. The product was extracted with dichloromethane. The combined organic layers were washed with brine, dried over potassium carbonate, filtered and concentrated in vacuo. The crude product was purified by silica gel chromatography (0-15% methanol/CH2Cl2) to afford 0.08 g (45% yield) of the desired product AB: 1H NMR (400 MHz, CDCl3) δ 8.54-8.34 (m, 2H), 8.15 (bs, 1H), 7.71 (d, J=7.6 Hz, 1H), 7.60-7.52 (m, 1H), 7.45 (t, J=7.2 Hz, 2H), 7.39-7.32 (m, 1H), 7.32-7.22 (m, 2H), 7.14-7.04 (m, 1H), 7.02-6.84 (m, 1H), 4.15-4.04 (m, 1H), 3.92-3.70 (m, 4H), 2.82-2.50 (m, 6H), 2.15-2.04 (m, 1H), 2.04-1.82 (m, 1H), 1.82-1.78 (m, 1H), 1.70-1.40 (m, 5H); ESI+ MS: m/z (rel intensity) 468.3 (100, [M+H]+)




Example 13
Preparation of N1-(4,5-dihydro-1H-imidazol-2-yl)-N4((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-N4-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine (AC)
A heavy-walled Pyrex tube was charged with a solution of Z (0.19 g, 0.36 mmol) in Ethanol (2 mL). The solution was treated with Hunig's base (N,N-diisopropylethylamine) (0.25 mL, 1.45 mmol) and 2-methylthio-2-imidazoline hydroiodide (0.05 g, 0.40 mmol). The tube containing the resulting mixture was sealed with an aluminum crimp cap fitted with a silicon septum and then it was exposed to microwave irradiation for 10 min at a temperature of 150° C. After the irradiation, the reaction tube was cooled with high-pressure air until the temperature had fallen below 40° C. The crude reaction mixture was concentrated in vacuo. The crude product was purified by silica gel chromatography (0-10% methanol/CH2Cl2) to afford 0.07 g (44% yield) of the desired product AC: 1H NMR (400 MHz, CDCl3) δ 9.13 (bs, 1H), 8.80 (t, J=5.6 Hz, 1H), 8.30 (d, J=3.6 Hz, 1H), 8.09 (s, 1H), 7.93 (bs, 1H), 7.75-7.68 (m, 2H), 7.48 (d, J=7.2 Hz, 2H), 7.44-7.32 (m, 2H), 7.05 (dd, J=4.8, 7.6 Hz, 1H), 4.12 (dd, J=6.0, 9.6 Hz, 1H), 4.00 (d, J=14.4 Hz, 1H), 3.79 (d, J=14.4 Hz, 1H), 3.40-3.22 (m, 2H), 2.80-2.48 (m, 4H), 2.20-2.10 (m, 1H), 2.05-1.90 (m, 1H), 1.90-1.45 (m, 6H); ESI+ MS: m/z (rel intensity) 445.2 (100, [M+H]+).







Example 14
Preparation of N-((1-(2-(aminomethyl)phenyl)-1H-1,2,3-triazol-4-yl)methyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine tetrahydrochloride (AD)
Step a: Preparation of tert-butyl 2-aminobenzylcarbamate (11). To a THF (30 mL) solution of 2-aminobenzyl amine (2.0 g, 16.4 mmol) was added triethylamine (4.60 mL, 32.7 mmol) and di-tert-butyldicarbonate (4.3 g, 19.6 mmol). The reaction mixture was stirred at room temperature for 16 h. The crude reaction mixture was then poured over saturated aqueous NaHCO3 and the product was then extracted with ethyl acetate. The organic phase was washed with brine and then dried over MgSO4, filtered and concentrated in vacuo. The crude product was purified by silica gel chromatography (0-5% methanol/dichloromethane) yielding 730 mg (20%) of 11: 1H NMR (400 MHz, CDCl3) δ 7.12-7.80, (m, 1H), 7.00 (d, J=7.6 Hz, 1H), 6.69-6.62 (m, 2H), 4.77, (bs, 1H), 4.29-4.11, (m, 4H), 1.43 (s, 9H); ESI+ MS: m/z (rel intensity) 223 (80, M+H).

Step b: Preparation of tert-butyl 2-azidobenzylcarbamate (12). To a acetonitrile (40 mL) solution of 15 (5.00 g, 22.5 mmol) at 0° C. was added tert-butylnitrite (4.00 mL, 33.7 mmol) and trimethylsilylazide (33.55 mL, 26.9 mmol). The reaction stirred at 0° C. for 3 h. The reaction mixture was concentrated in vacuo, and the crude material was taken on to the next step without further purification. ESI+ MS: m/z (rel intensity) 271 (80, M+H).

Step c: Preparation of tert-butyl 2-(4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl)benzylcarbamate (13). tert-butyl 2-azidobenzylcarbamate, 12, (5.4 g, 21.7 mmol) was dissolved in neat propargyl alcohol (10 mL). The reaction mixture was stirred at 90° C. for 18 h. The reaction mixture was then concentrated in vacuo and the resulting residue dissolved in diethyl ether. Upon standing, triazole 13 crystallized. The crystals were collected by filtration yielding 2.5 g (38%) of 13: 1H NMR (400 MHz, CDCl3) δ 7.82, (s, 1H), 7.64-7.35 (m, 3H), 7.34-7.30 (m, 1H), 5.35, (bs, 1H), 4.87, (s, 2H), 4.15 (s, 2H), 1.42 (s, 9H); ESI+ MS: m/z (rel intensity) 249 (100, M-tBu).

Step d: Preparation of tert-butyl 2-(4-formyl-1H-1,2,3-triazol-1-yl)benzylcarbamate (14). To a solution of tert-butyl 2-(4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl)benzylcarbamate, 13, (2.5 g, 8.31 mmol) in CH2Cl2 (10 mL) was added MnO2 (5.0 g, 57.5 mmol). The reaction mixture was stirred at room temperature for 18 h. The crude reaction mixture was then filtered through a plug of Celite® and then concentrated in vacuo. The crude material was purified by silica gel chromatography (0-5% methanol/dichloromethane) yielding 610 mg (25%) of 14: ESI+ MS: m/z (rel intensity) 225 (100, M+Na).

Step e: Preparation of tert-butyl(2-(4-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)-1H-1,2,3-triazol-1-yl)phenyl) (15). To a solution of tert-butyl 2-(4-formyl-1H-1,2,3-triazol-1-yl)benzylcarbamate, 14, (0.61 g, 2.03 mmol) in 1,2-dichloroethane (4 mL) was added N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 11, (0.30 g, 1.85 mmol), sodium triacetoxyborohydride (0.70 g, 3.33 mmol), and AcOH (10 drops). The reaction mixture was heated to 65° C. and stirred for 18 h. The reaction mixture was poured into saturated aqueous NaHCO3 (10 mL) and was extracted with EtOAc (2×10 mL). The organic phases were combined and washed with brine, dried over MgSO4, filtered, and concentrated in vacuo. The crude material was purified by silica gel chromatography (0-5% methanol/dichloromethane) yielding 360 mg (44%) of 15: 1H NMR (400 MHz, CDCl3) δ 8.48 (d, J=4.4 Hz, 1H), 8.04 (s, 1H), 7.79 (bs, 1H), 7.64 (d, J=7.6 Hz, 1H), 7.47-7.30 (m, 4H), 7.08 (dd, J=8.0, 4.8 Hz, 1H), 4.18-3.95 (m, 5H), 2.88-2.65, (m, 2H), 2.41, (s, 3H), 2.24-2.14, (m, 1H), 2.10-1.90, (m, 2H), 1.76-1.64 (m, 1H), 1.39 (s, 9H); ESI+ MS: m/z (rel intensity) 449 (100, M+H).

Step f: Preparation of N-((1-(2-(aminomethyl)phenyl)-1H-1,2,3-triazol-4-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (AD). Through a methanol (2 mL) solution of tert-butyl(2-(4-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)-1H-1,2,3-triazol-1-yl)phenyl), 15, (0.36 g, 0.80 mmol) was bubbled HCl(g) (generated by adding conc. H2SO4 to dry NaCl) until complete conversion was observed by LC-MS. The reaction mixture was then concentrated in vacuo yielding 250 mg (68%) of AD: 1H NMR (400 MHz, d6-DMSO) δ 8.81-8.68, (m, 4H), 8.52, (d, J=4.4 Hz, 1H), 7.87, (d, J=6.8 Hz, 1H), 7.75-7.50, (m, 4H), 7.38, (dd, J=7.6, 4.8 Hz, 1H), 4.81-4.73, (m, 1H), 4.61, (d, J=13.6 Hz, 1H), 4.47, (d, J=13.6 Hz, 1H), 3.91-3.83, (m, 2H), 2.90-2.70, (m, 5H), 2.51-2.49, (m, 1H), 2.15-2.03, (m, 2H), 1.72-1.60, (m, 1H); ESI+ MS: m/z (rel intensity) 349 (100, M+H).





Example 15
Preparation of N-methyl-N-((1-(2-((pyridin-2-ylmethylamino)methyl)-phenyl)-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (AG)
To a solution of N-((1-(2-(aminomethyl)phenyl)-1H-1,2,3-triazol-4-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride, AD, (0.20 g, 0.43 mmol) in methanol (2 mL) was added 2-pyridine carboxaldehyde (0.04 mL, 0.43 mmol) and triethylamine (0.18 mL, 1.30 mmol). The reaction mixture was heated to 60° C. and stirred for 18 h. The reaction mixture was then cooled to room temperature and NaBH4 (0.05 g, 1.30 mmol) was added. The reaction stirred 1 h at room temperature. The reaction mixture was poured into brine (10 mL) and was extracted with ethyl acetate (2×10 mL). The combined organic phases were dried over MgSO4, filtered, and concentrated in vacuo. The crude product was purified by chromatography on basic alumina (0-5% methanol/dichloromethane) yielding AG. ESI+ MS: m/z (rel intensity) 440 (100, M+H).




Example 16
Preparation of N-methyl-N-((1-(piperidin-4-ylmethyl)-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (AI) and N-methyl-N-((3-(piperidin-4-ylmethyl)-3H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (AK)
Step a: Preparation of tert-butyl 4-(azidomethyl)piperidine-1-carboxylate (16). To a solution of tert-butyl 4-(hydroxymethyl)piperidine-1-carboxylate (2.00 g, 9.28 mmol) in CH2Cl2 (40 mL) was added methanesulfonyl chloride (0.76 mL, 9.75 mmol) followed by triethylamine (1.43 mL, 10.21 mmol). The reaction mixture was stirred at room temperature for 3 h. The mixture was diluted with NaHCO3 and extracted with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude solid was used without further purification: 1H NMR (d6-DMSO) δ 4.02 (d, J=6.8 Hz, 2H), 3.95-3.85 (m 2H), 3.13 (s, 3H), 2.75-2.60 (m, 2H), 1.85-1.77 (m, 1H), 1.61 (d, J=13.2 Hz, 2H), 1.35 (s, 9H), 1.04 (ddd, J=12.4, 12.4, 4.4 Hz, 2H).

Step b: To a solution of crude tert-butyl 4-((methylsulfonyloxy)methyl)piperidine-1-carboxylate (0.50 g, 1.71 mmol) in dimethylformamide (6 mL) was added sodium azide (0.14 g, 2.22 mmol). The reaction mixture was heated to 70° C. and stirred for 17 h. The mixture was diluted with water, extracted with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude solid was used without further purification: 1H NMR (d6-DMSO) δ 3.22 (d, J=6.8 Hz, 2H), 2.68-2.55 (m 2H), 1.67-1.57 (m, 3H), 1.35 (s, 9H), 0.99 (ddd, J=12.4, 12.4, 4.4 Hz, 2H); ESI+ MS: m/z (rel intensity) 263.1 (5, [M+H]+).

Step c: Preparation of N-methyl-N-(prop-2-ynyl)-5,6,7,8-tetrahydroquinolin-8-amine (17). To a solution of N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.50 g, 3.08 mmol) in THF (15 mL) was added propargyl bromide (0.28 mL, 3.08 mmol), sodium iodide (0.05 g, 0.31 mmol) and sodium carbonate (0.98 g, 9.24 mmol). The reaction mixture was stirred at room temperature for 3 h. The mixture was diluted with water, extracted with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude solid was used without further purification: 1H NMR (d6-DMSO) δ 8.30 (d, J=4.4 Hz, 1H), 7.44 (d, J=8.0 Hz, 1H), 7.13 (dd, J=8.0, 5.2 Hz, 1H), 3.74 (dd, J=4.8, 4.8 Hz, 1H), 3.47 (ddd, J=16.8, 2.4, 2.4 Hz, 2H), 3.06 (s, 1H), 2.80-2.60 (m, 2H), 2.12 (s, 3H), 2.12-1.87 (m, 2H), 1.74-1.68 (m, 1H), 1.62-1.55 (m, 1H); ESI+ MS: m/z (rel intensity) 201.1 (100, [M+H]+).

Step d: Preparation of tert-butyl 4-((4-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)piperidine-1-carboxylate (18) and tert-butyl 4-((5-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)piperidine-1-carboxylate (19). A neat mixture of N-methyl-N-(prop-2-ynyl)-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.31 g, 1.54 mmol) and tert-butyl 4-(azidomethyl)piperidine-1-carboxylate, 16, (0.37 g, 1.54 mmol) was heated in microwave at 150° C. for 20 min. The resulting residue was partitioned between aqueous saturated NaHCO3 solution and CH2Cl2. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude solid was purified by column chromatography (basic alumina, 0% methanol/CH2Cl2 to 20% methanol/CH2Cl2) to afford a 1.4 to 1 ratio of the 1,4-substituted (18) and 1,5-substituted (19) triazole regioisomers: 1H NMR (d6-DMSO) δ 8.36 (d, J=5.6 Hz, 1H), 7.90 (s, 1H), 7.44 (dd, J=7.6 Hz, 1H), 4.27-4.24 (m, 1H), 4.22 (d, J=7.2 Hz, 1H), 3.88-3.77 (m, 2H), 3.88-3.77 (m, 4H), 2.72-2.07 (m, 4H), 1.95-1.85 (m, 4H), 1.64-1.55 (m, 1H), 1.42-1.35 (m, 1H), 1.35 (s, 9H), 1.05-0.98 (m, 1H); ESI+ MS: m/z (rel intensity) 441.2 (100, [M+H]+).

Step e: Preparation of N-methyl-N-((1-(piperidin-4-ylmethyl)-1H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (AI) and N-methyl-N-((3-(piperidin-4-ylmethyl)-3H-1,2,3-triazol-4-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (AK). To a mixture of triazole regioisomers 18 and 19 (0.18 g, 0.42 mmol) in methanol (10 mL) was bubbled HCl gas (generated from H2SO4 and NaCl). The reaction mixture was stirred at room temp. for 30 minutes. The mixture was concentrated in vacuo to afford a 1.3 to 1 molar ratio of triazoles AI and AK. The tetrahydrochloride salt was used without further purification: ESI+ MS: m/z (rel intensity) 341.2 (100, [M+H]+).








Example 17
Preparation of N-((1-(3-aminopropyl)-1H-1,2,3-triazol-4-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (W)
Step a: Preparation of 2-(3-azidopropyl)isoindoline-1,3-dione (20). A heavy-walled Pyrex tube was charged with a suspension of N-(3-bromopropyl)phthalimide (1.00 g, 3.72 mmol) and sodium azide (0.32 g, 4.84 mmol) in H2O (2 mL). The tube containing the resulting mixture was sealed with an aluminum crimp cap fitted with a silicon septum and then it was exposed to microwave irradiation for 30 min at a temperature of 120° C. After the irradiation, the reaction tube was cooled with high-pressure air until the temperature had fallen below 40° C. The product was extracted with ethyl acetate. The combined organic layers were dried with magnesium sulfate, filtered and concentrated in vacuo. The crude material (0.62 g, 72% yield) was used without further purification in the next step. 1H NMR (400 MHz, CDCl3) δ 7.86-7.83 (m, 2H), 7.73-7.70 (m, 2H), 3.77 (t, J=6.4 Hz, 2H), 3.36 (t, J=8.0 Hz, 2H), 1.94 (m, 2H).

Step b: Preparation of 2-(3-(4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl)propyl)-isoindoline-1,3-dione (21). A solution of 2-(3-azidopropyl)isoindoline-1,3-dione, 20, (0.62 g, 2.69 mmol) in propargyl alcohol (4 mL) was warmed to 90° C. and stirred for 18 h. The reaction mixture was concentrated under reduced pressure. The crude product containing both regioisomers ( ̃1:1 ratio) was used in the next step without further purification. ESI+ MS: m/z (rel intensity) 301.1 (100, [M+H]+).

Step c: Preparation of 1-(3-(1,3-dioxoisoindolin-2-yl)propyl)-1H-1,2,3-triazole-4-carbaldehyde (22). A solution of the regioisomers 21 (0.77 g, 2.69 mmol) in dichloromethane (10 mL) was treated with manganese dioxide (0.94 g, 10.77 mmol). The resulting mixture was stirred at room temperature for 2 h. The reaction mixture was filtered through Celite and concentrated. The crude product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.24 g (31% yield) of 22 as a single regioisomer: 1H NMR (400 MHz, CDCl3) δ 10.08 (s, 1H), 8.34 (s, 1H), 7.85-7.65 (m, 4H), 4.45 (t, J=12.0 Hz, 2H), 3.77-3.71 (m, 2H), 2.42-2.34 (m, 2H); ESI+ MS: m/z (rel intensity) 285.0 (100, [M+H]+).

Step d: Preparation of 2-(3-(4-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)-methyl)-1H-1,2,3-triazol-1-yl)propyl)isoindoline-1,3-dione (AH). A solution of 1-(3-(1,3-dioxoisoindolin-2-yl)propyl)-1H-1,2,3-triazole-4-carbaldehyde, 22, (0.24 g, 0.84 mmol), N-methyl-N-(prop-2-ynyl)-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.17 g, 1.01 mmol), sodium triacetoxyborohydride (0.27 g, 1.26 mmol) and a catalytic amount of acetic acid (2 drops) in 1,2-dichloroethane (10 mL) was warmed to 65° C. and stirred at this temperature for 24 h. The reaction mixture was cooled to room temperature. A saturated aqueous solution of sodium bicarbonate was added. The product was extracted with dichloromethane. The combined organic layers were dried over potassium carbonate, filtered and concentrated. The crude product was purified by silica gel chromatography (0-5% methanol/CH2Cl2) to afford 0.19 g (80% yield) of the desired product, AH: 1H NMR (400 MHz, CDCl3) δ 8.45 (d, J=4.4 Hz, 1H), 7.79-7.76 (m, 2H), 7.71 (s, 1H), 7.68-7.65 (m, 2H), 7.30 (d, J=7.6 Hz, 1H), 7.00 (dd, J=4.8, 8.0 Hz, 1H), 4.32 (dt, J=3.2, 7.2 Hz, 2H), 3.91 (dd, J=6.0, 9.2 Hz, 1H), 3.77-3.66 (m, 4H), 2.82-2.70 (m, 1H), 2.70-2.60 (m, 1H), 2.29 (s, 3H), 2.29-2.23 (m, 2H), 2.12-2.02 (m, 1H), 2.02-1.92 (m, 1H), 1.92-1.80 (m, 1H), 1.70-1.56 (m, 1H); ESI+ MS: m/z (rel intensity) 431.2 (100, [M+H]+).

Step e: Preparation of N-((1-(3-aminopropyl)-1H-1,2,3-triazol-4-yl)methyl)-N-methyl-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (W). A solution of 2-(3-(4-((methyl(5,6,7,8-tetrahydroquinolin-8-yl)amino)-methyl)-1H-1,2,3-triazol-1-yl)propyl)isoindoline-1,3-dione, AH, (0.16 g, 0.37 mmol) in ethanol (5 mL) was treated with hydrazine (23 μL, 0.74 mmol). The resulting mixture was warmed to 80° C. and stirred for 20 h. The reaction mixture was cooled to room temperature. The white precipitate that formed was filtered and the filtrate was concentrated in vacuo. Chloroform was added. More of the precipitate crashed out and was filtered off. The solution was concentrated and dried under reduced pressure to afford 0.08 g (72% yield) of product W: 1H NMR (400 MHz, CDCl3) δ 8.47 (d, J=3.6 Hz, 1H), 7.65 (s, 1H), 7.33 (d, J=7.6 Hz, 1H), 7.02 (d, J=4.8, 8.0 Hz, 1H), 4.39 (t, J=7.2 Hz, 1H), 3.95 (dd, J=5.6, 9.2 Hz, 1H), 3.75 (q, J=13.6 Hz, 2H), 2.85-2.60 (m, 2H), 2.68 (t, J=6.8 Hz, 2H), 2.31 (s, 3H), 2.25-2.05 (m, 2H), 2.05-1.95 (m, 2H), 1.95-1.80 (m, 1H), 1.75-1.58 (m, 1H); ESI+ MS: m/z (rel intensity) 301.2 (95, [M+H]+).

Example 18
General Preparation of Compounds of Formulae (IB) and (IC) in which R5 and R6 are both H and L2Y is a Substituted or Unsubstituted Aminoalkyl
Compounds of Formula (IB) and (IC) in which R5 and R6 are both H and L2Y is a substituted or unsubstituted aminoalkyl can be prepared, for example, as generally described in Scheme 14 below. The skilled artisan will recognize that substituted aminoalkyl compounds can be prepared by the appropriate derivatization of the unsubstituted aminoalkyl compound which is prepared according to Scheme 14. Other modifications (e.g., modifying the chain length of the alkylene portion of the aminoalkyl group, functionalization of the alcohol intermediate, etc.) are prepared by providing modified intermediates of Schemes 6-10.







Example 19
General Preparation of Compounds of Formula (ID)
Compounds of Formula (I) can have the following general structure (1D):




wherein L1, L2, L3, X, Y, Z, G, J, and A are as described or defined herein. Specific embodiments of the compounds of Formula (ID) are shown in Table 3, below.













TABLE 3





Com- pound
—L1—X
—G
—L3—Z
—J
—L2—Y







AY
H
—




CONH






 


AZ
H
—




CONH






 


BA
H
—




CONH






 


BB
H
—




CONH






 


BC
H
—




CONH






 


BD
H
—




CONH






 


BE
H
—




CONH






 


BF
H
CONH




—






 


BG
H
CONH




—






 


BH
H
CONH




—






 


BI
H
CONH




—






 


BJ
H
—




CO






 


BK
H
—




CO






 


BL
H
—




CO






 


BM
H
—




CO






 


BN
H
—




CO






 


BO
H
—




CO






 


BO1
H
CO




—






 


BO2
H
CO




—






 


BO3
H
CO




—






 


BO4
H
CO




—




















Example 20
Preparation of (S)-5-(diisobutylamino)-N-((R)-1-(naphthalen-1-yl)ethyl)-2-(5,6,7,8-tetrahydroquinolin-8-ylamino)pentanamide trihydrochloride (AY)
Step a: Preparation of benzyl (S)-4-(tert-butoxycarbonyl)-amino-5-((S)-1-(naphthalen-1-yl)ethylamino)-5-oxopentylcarbamate (23). To a solution of Boc-Orn(Z)—OH (Bachem) (51.6 g, 141.0 mmol) in ethyl acetate (800 mL) was added 1,1-carbonyl diimidazole (24.0 g, 148.0 mmol). The mixture was stirred at room temp. for 30 min. A solution of(S)-(−)-(1)-(1-naphthyl)ethyl amine (25.3 g, 148.0 mmol) in CHCl3 was added to the reaction mixture dropwise. The reaction mixture was stirred at room temperature for an additional 1 h. The mixture was diluted with water, extracted with ethyl acetate. The organic phase was washed with 0.3 N HCl (500 mL), water and then aqueous saturated NH4Cl solution. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (0% to 20% methanol/CHCl3) to afford the desired product 23: ESI+ MS: m/z (rel intensity) 520 (100, [M+H]+).

Step b: Preparation of tert-butyl(S)-5-amino-1-((S)-1-(naphthalen-1-yl)ethylamino)-1-oxopentan-2-ylcarbamate (24). A solution of (S)-4-(tert-butoxycarbonyl)-amino-5-((S)-1-(naphthalen-1-yl)ethylamino)-5-oxopentylcarbamate, 23, (28.0 g, 54.0 mmol) in ethanol (600 mL) was added ammonium formate (10.2 g, 160.0 mmol) and palladium (10 wt % on carbon, 1.0 g). The reaction mixture was warmed to 55° C. and stirred for 18 h at 55° C. The mixture was filtered through Celite and washed with ethanol. The resulting filtrate was concentrated in vacuo. The residue was dissolved in ethyl acetate and the organic phase was washed with aqueous saturated NaCl solution. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was used without further purification: 1H NMR (d6-DMSO) δ 8.55 (d, J=7.6 Hz, 1H), 8.04 (d, J=8.4 Hz, 1H), 7.89 (d, J=8.0 Hz, 1H), 7.78 (d, J=8.0 Hz, 1H), 7.53-7.42 (m, 4H), 6.81 (d, J=8.0 Hz, 1H), 5.65-5.62 (m, 1H), 3.96-3.89 (m, 1H), 2.60-2.53 (m, 2H), 1.46-1.40 (m, 7H), 1.33 (s, 9H); ESI+ MS: m/z (rel intensity) 386.2 (100, [M+H]+).

Step c: Preparation of tert-butyl(S)-5-(diisobutylamino)-1-((S)-1-(naphthalen-1-yl)ethylamino)-1-oxopentan-2-ylcarbamate (25). To a solution of tert-butyl(S)-5-amino-1-((S)-1-(naphthalen-1-yl)ethylamino)-1-oxopentan-2-ylcarbamate, 24, (20.8 g, 54.0 mmol) in CH2Cl2 (600 mL) was added acetic acid (conc. 1 mL), followed by isobutyraldehyde (10.8 mL, 120.0 mmol) and sodium triacetoxyborohydride (28.6 g, 135 mmol). The reaction mixture was warmed to 65° C. and stirred for 30 min. The mixture was diluted with water, extracted with ethyl acetate. The organic phase was washed with aqueous saturated NaCl. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was used without further purification: 1H NMR (d6-DMSO) δ 8.39 (d, J=7.6 Hz, 1H), 8.04 (d, J=7.2 Hz, 1H), 7.89 (d, J=8.0 Hz, 1H), 7.77 (d, J=8.0 Hz, 1H), 7.51-7.39 (m, 4H), 6.68 (d, J=8.4 Hz, 1H), 5.68-5.60 (m, 1H), 3.97-3.90 (m, 1H), 2.41-2.22 (m, 2H), 1.91 (d, J=7.2 Hz, 2H), 1.58-1.52 (m, 2H), 1.44 (d, J=6.8 Hz, 3H), 1.33 (s, 9H), 0.75 (d, J=6.8 Hz, 12H); ESI+ MS: m/z (rel intensity) 498.3 (100, [M+H]+).

Step d: Preparation of (S)-2-amino-5-(diisobutylamino)-N—((S)-1-(naphthalen-1-yl)ethyl)pentanamide (26). To a solution of tert-butyl(S)-5-(diisobutylamino)-1-((S)-1-(naphthalen-1-yl)ethylamino)-1-oxopentan-2-ylcarbamate (2.0 g, 4.0 mmol) in CH2Cl2 (35 mL) was added trifluoroacetic acid (20 mL) dropwise. After stirring the reaction mixture for 45 min at room temperature, the mixture was concentrated in vacuo to afford the trifluoroacetic acid salt. The crude salt was used without further purification: ESI+ MS: m/z (rel intensity) 398.3 (100, [M+H]+).

Steps e and f: Preparation of (S)-5-(diisobutylamino)-N—((R)-1-(naphthalen-1-yl)ethyl)-2-(5,6,7,8-tetrahydroquinolin-8-ylamino)pentanamide trihydrochloride (AY). To a solution of (S)-2-amino-5-(diisobutylamino)-N—((S)-1-(naphthalen-1-yl)ethyl)-pentanamide, 26, (0.60 g, 1.51 mmol) in ethanol (4 mL) was added 6,7-dihydroquinolin-8(5H)-one (0.21 g, 1.43 mmol). The mixture was heated in microwave reactor at 150° C. for 10 min. After cooling the reaction to room temp., NaBH4 (0.11 g, 2.86 mmol) was added to the mixture. The reaction mixture was stirred at room temp for 1 h. The mixture was diluted with aqueous saturated NaHCO3, extracted with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (0% to 5% methanol/CH2Cl2) to afford the desired product: 1H NMR (d6-DMSO) δ 8.34 (d, J=4.8 Hz, 1H), 8.30-8.25 (m, 1H), 8.11-8.05 (m, 1H), 7.92-7.87 (m, 1H), 7.80-7.76 (m, 1H), 7.52-7.38 (m, 5H), 7.17-7.11 (m, 1H), 5.74-5.67 (m, 1H), 4.50 (d, J=4.0 Hz, 1H), 2.71-2.55 (m, 4H), 2.95 (m, 1H), 2.05-1.80 (m, 6H), 1.58-1.40 (m, 11H), 0.76 (d, J=6.4 Hz, 12H); ESI+ MS: m/z (rel intensity) 529.3 (100, [M+H]+).








Example 21
Preparation of (2S)-6-amino-N-(pyridin-2-ylmethyl)-2-(5,6,7,8-tetrahydroquinolin-8-ylamino)hexanamide tetrahydrochloride (AZ)
Step a: Preparation of (S)-benzyl 6-((tert-butoxycarbonyl)-amino)-1-oxo-1-(pyridin-2-ylmethylamino)hexan-2-ylcarbamate (27). To a cold (0° C.) solution of Boc-Lys(Z)—OH (Bachem, 10.0 g, 26.3 mmol) in dimethylformamide (80 mL) was added hydroxybenzotriazole-hydrate (4.3 g, 31.6 mmol), triethylamine (7.3 mL, 52.6 mmol) and EDAC (6.1 g, 31.6 mmol). After stirring at 0° C. for 1 h, 2-aminomethylpyridine (3.0 mL, 28.9 mmol) was added to the mixture. The mixture was warmed to room temperature and stirred for an additional 17 h. The mixture was diluted with aqueous saturated NaHCO3 and extracted with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (0% to 5% methanol/CHCl3) to afford the desired product 27: 1H NMR (d6-DMSO) δ 8.43 (d, J=4.0 Hz, 1H), 8.39 (t, J=5.6 Hz, 1H), 7.68 (t, J=7.6 Hz, 1H), 7.34-7.19 (m, 8H), 6.94 (d, J=7.6 Hz, 1H), 4.96 (s, 2H), 4.31 (d, J=5.6 Hz, 2H), 3.90-3.83 (m, 1H), 2.95-2.90 (m, 2H), 1.60-1.25 (m, 6H), 1.35 (s, 9H); ESI+ MS: m/z (rel intensity) 471.2 (100, [M+H]+).

Step b: Preparation of (S)-tert-butyl-5-amino-6-oxo-6-(pyridin-2-ylmethyl-amino)hexylcarbamate (28). To a solution of (S)-benzyl 6-((tert-butoxycarbonyl)-amino)-1-oxo-1-(pyridin-2-ylmethylamino)hexan-2-ylcarbamate, 27, (3.8 g, 8.2 mmol) in methanol (80 mL) was added palladium (10 wt % on carbon, 0.5 g). The mixture was stirred under a hydrogen atmosphere for 2 h. The mixture was filtered through Celite and washed with methanol. The resulting filtrate was concentrated in vacuo. The crude residue was used without further purification: ESI+ MS: m/z (rel intensity) 337.2 (100, [M+H]+).

Steps c and d: Preparation of tert-butyl(S)-6-oxo-6-(pyridin-2-ylmethylamino)-5-(5,6,7,8-tetrahydroquinolin-8-ylamino)hexylcarbamate (29). To a solution of (S)-tert-butyl 5-amino-6-oxo-6-(pyridin-2-ylmethyl-amino)hexylcarbamate, 28, (2.16 g, 6.43 mmol) in ethanol (14 mL) was added 6,7-dihydroquinolin-8(5H)-one (0.90 g, 6.12 mmol). The mixture was heated in microwave reactor at 150° C. for 13 min. After cooling the reaction to room temp., NaBH4 (0.46 g, 12.24 mmol) was added to the mixture. The reaction mixture was stirred at room temp for 1 h. The mixture was diluted with aqueous saturated NaHCO3, extracted twice with ethyl acetate. The organic phase was dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (5% to 25% methanol/CH2Cl2) to afford the desired product 29: 1H NMR (d6-DMSO) δ 8.44 (d, J=4.4 Hz, 1H), 8.40 (m, 1H), 8.32-8.31 (m, 1H), 7.66 (t, J=7.2 Hz, 1H), 7.45 (d, J=7.6 Hz, 1H), 7.24 (d, J=8.0 Hz, 1H), 7.20 (t, J=4.8 Hz, 1H), 7.13 (dd, J=8.0, 4.2 Hz, 1H), 6.94 (d, J=7.6 Hz, 1H), 4.32 (d, J=5.6 Hz, 2H), 3.95-3.89 (m, 1H), 3.62-3.57 (m, 1H), 2.69 (d, J=4.8 Hz, 2H), 2.61-2.56 (m, 2H), 1.99-1.95 (m, 1H), 1.89-1.83 (m, 1H), 1.63-1.28 (m, 8H), 1.35 (s, 9H); ESI+ MS: m/z (rel intensity) 468.3 (100, [M+H]+).

Step e: Preparation of (2S)-6-amino-N-(pyridin-2-ylmethyl)-2-(5,6,7,8-tetrahydro-quinolin-8-ylamino)hexanamide tetrahydrochloride (AZ). To a solution of tert-butyl(S)-6-oxo-6-(pyridin-2-ylmethylamino)-5-(5,6,7,8-tetrahydroquinolin-8-ylamino)-hexylcarbamate, 29, (1.07 g, 2.29 mmol) in methanol (50 mL) was bubbled HCl gas (generated from H2SO4 and NaCl). The reaction mixture was stirred at room temp. for 20 minutes. The mixture was concentrated in vacuo to afford the desired product. The tetrahydrochloride salt was used without further purification: ESI+ MS: m/z (rel intensity) 368.2 (100, [M+H]+).

Example 22
Preparation of Compound BI, of a Compound of Formula (ID)
Compounds of Formula (ID), such as compound BI, are prepared using the procedure of Scheme 17. The skilled artisan will recognize that various modifications of such compounds are possible by providing modified intermediates. For example, different heterocyclyls corresponding to group Y can be incorporated into the structure by the selection of heterocyclyl in the starting material in step 1 of Scheme 8, different alkylene groups corresponding to L1 can be provided by the choice of diamine (or diamine synthon) using in step 1 of Scheme 7, etc.







Example 23
General Preparation of Compounds of Formula (IE)
Compounds of Formula (I) can have the following general structure (1E):




wherein R1, R2, R7, L1, L2, X, Y, and A are as defined herein. Exemplary compounds of Formula (IE) are shown in Table 4.












TABLE 4





Compd.
—R7
—L1—X
—L2—Y











BP
CH3
CH3










 


BQ
CH3














 


BR
CH3














 


BS
CH3














 


BT
CH3














 


BU




CH3










 


BV




CH3










 


BW
CH3
CH3










 


BX
CH3
CH3










 


BY
CH3
CH3










 


BZ
CH3
CH3










 


CA
CH3
CH3





















Example 24
Preparation of N-methyl-N-((4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (BP)
Step a: Preparation of (4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methanol (35). To a o-xylene (20 mL) solution of 4-methyl-3-phenyl-4H-1,2,4-triazole, 34, (Ivanova, N. V.; Sviridov, S. I.; Shorshnev, S. V.; Stepanov, A. E. Synthesis, 2006, 1, 156; herein incorporated by reference in its entirety for all purposes) (3.0 g, 18.8 mmol) was added p-formaldehyde (2.8 g, 94.2 mmol). The reaction mixture was heated to 120° C. for 3 h. The reaction mixture was then cooled to room temperature and was diluted into chloroform (40 mL) and was washed with water (30 mL). The organics were dried over MgSO4, filtered, and concentrated in vacuo. The crude material was purified by silica get chromatography (0-5% methanol/dichloromethane) yielding 1.3 g (37%) of 35: 1H NMR (400 MHz, CDCl3) δ 7.56-7.52 (m, 2H), 7.48-7.40 (m, 3H), 5.40 (bs, 1H), 4.83 (s, 2H), 3.74 (s, 3H); ESI+ MS: m/z (rel intensity) 190 (100, M+H).

Step b: Preparation of 4-methyl-5-phenyl-4H-1,2,4-triazole-3-carbaldehyde (36). To a solution of (4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methanol, 35, (1.3 g, 6.9 mmol) in THF (20 mL) was added MnO2 (6.0 g, 68.7 mmol). The reaction stirred at room temperature for 2 h. The reaction mixture was then filtered through a plug of Celite® and concentrated in vacuo. The crude material was purified via silica gel chromatography (0-5% methanol/dichloromethane) yielding 820 mg (64%) of 36: 1H NMR (400 MHz, CDCl3) δ 10.14, (s, 1H), 7.68-7.60 (m, 2H), 7.59-7.45, (m, 3H), 3.98 (s, 3H); ESI+ MS: m/z (rel intensity) 188 (100, M+H).

Step c: Preparation of N-methyl-N-((4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine (BP). To a solution of N-methyl-5,6,7,8-tetrahydroquinolin-8-amine, 5, (0.39 g, 2.40 mmol) in 1,2-dichloroethane (4 mL) was added 4-methyl-5-phenyl-4H-1,2,4-triazole-3-carbaldehyde, 36, (0.41 g, 2.20 mmol), sodium triacetoxyborohydride (0.84 g, 3.90 mmol), and AcOH (1 drop). The reaction mixture was heated to 65° C. and stirred for 18 h. The reaction mixture was cooled to room temperature, poured into saturated aqueous NaHCO3 (10 mL) and extracted with ethyl acetate (2×10 mL). The combined organic phases were washed with brine then dried over MgSO4, filtered, and concentrated in vacuo. The crude material was purified via chromatography on basic alumina (0-5% methanol/dichloromethane) yielding BP: 1H NMR (400 MHz, CDCl3) δ 8.39, (d, J=4.8 Hz, 1H), 7.58-7.53 (m, 2H), 7.46-7.39, (m, 3H), 7.32, (d, J=8.4 Hz, 1H), 7.01, (dd, J=7.2, 4.4 Hz, 1H), 4.04-3.92, (m, 2H), 3.78, (d, J=14.0 Hz, 1H), 2.85-2.74, (m, 1H), 2.71-2.62 (m, 1H), 3.33, (s, 3H), 2.12-1.94 (m, 3H), 1.74-1.62 (m, 1H); ESI+ MS: m/z (rel intensity) 334 (100, M+H).





Example 25
Preparation of tert-butyl 4-(((4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methyl)(5,6,7,8-tetrahydroquinolin-8-yl)amino)butylcarbamate (37)
Step f: To a solution of 4-methyl-5-phenyl-4H-1,2,4-triazole-3-carbaldehyde, 36, (0.41 g, 2.20 mmol) in 1,2-dichloroethane (4 mL) was added tert-butyl 4-(5,6,7,8-tetrahydroquinolin-8-ylamino)butylcarbamate, 3, (0.76 g, 2.40 mmol), sodium triacetoxyborohydride (0.84 g, 3.95 mmol), and AcOH (10 drops). The reaction mixture was heated to 65° C. and stirred for 18 h. The reaction mixture was cooled to room temperature and poured into saturated aqueous NaHCO3 (15 mL) and extracted with ethyl acetate (2×10 mL). The combined organic phases were washed with brine then dried over MgSO4, filtered, and concentrated in vacuo. The crude material was purified by silica gel chromatography (0-5% methanol/dichloromethane) to yield 37: 1H NMR (400 MHz, CDCl3) δ 8.40, (d, J=5.2 Hz, 1H), 7.60-7.57 (m, 2H), 7.48-7.44, (m, 3H), 7.31, (d, J=7.2 Hz, 1H), 7.0, (dd, J=8.0, 5.2 Hz, 1H), 5.01, (bs, 1H), 4.20-3.92, (m, 3H), 3.20-2.60, (m, 6H), 2.20-1.85, (m, 4H), 1.75-1.30 (m, 13H); ESI+ MS: m/z (rel intensity) 491 (100, M+H).

Step g: Preparation of N-(4-aminobutyl)-N-((4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)methyl)-5,6,7,8-tetrahydroquinolin-8-amine tetrahydrochloride (BQ). Through a methanol (2 mL) solution of 37 (0.16 g, 0.32 mmol) was bubbled HCl(g) generated by the addition of sulfuric acid to dry NaCl. Upon total conversion of the reaction as monitored by LC-MS, the reaction mixture was concentrated in vacuo to yield BQ: ESI+ MS: m/z (rel intensity) 391 (100, M+H).

The skilled practitioner will recognize that the remaining compounds of Table 4 can be prepared using similar methods using the appropriately substituted starting materials and reagents. Alternatively, substituted amine derivatives can be prepared by appropriate derivatization of the unsubstituted amine (e.g., BR-BT can be prepared from BQ, etc.).
Example 26
General Preparation of Compounds of Formula (IF)
Compounds of Formula (I) can have the following general structure (IF):




wherein R1, R2, L1, L2, X, Y, W, J, and A are as defined herein. Specific embodiments of the compounds of Formula (IF) are shown below in Table 5.













TABLE 5





Com- pound
—W—
—J—
—L1—X
—L2—Y











CB




—
CH3










 


CC




—
CH3










 


CD




—
CH3










 


CE




—
CH3










 


CF




—
CH3










 


CG




—
CH3










 


CH




—
CH3










 


CI




NH
CH3










 


CJ




NH
CH3










 


CK




NH
CH3










 


CL




NH
CH3










 


CM




NH
CH3










 


CN




NH
CH3










 


CO




CO
CH3










 


CP




CO
CH3










 


CQ




CO
CH3










 


CR




CO
CH3










 


CS




CO
CH3










 


CT




CONH
CH3










 


CU




CONH
CH3










 


CV




CONH
CH3










 


CW




CO
CH3










 


CX




CO
CH3










 


CY




CO
CH3

















The compounds of Formula (IF) generally can be prepared by the procedures of Schemes 20 and 21, below.

















The skilled practitioner will recognize that the various embodiments of the compounds of Formula (IF) disclosed in Table 5 can be prepared by analogous methods using appropriately substituted starting materials, or by appropriate modification e.g. of an amino-functional compound (e.g., compound CD can be prepared by di-alkylation of compound CC).
Example 27
General Preparation of Compounds of Formula (IG)
Compounds of Formula (I), can have the following general Formula (IG):




wherein R1, R2, R3, R4, L1, X, Q, A, and B are as defined herein. Specific embodiments of the compounds of Formula (IG) are shown in Table 6, below.












TABLE 6





Com- pound
—R3
—R4
Q
—L1—X























CZ




N










 












DA
H
H
CH










 


DB
H
H
CH
CH3






 











DC




N










 


DD




N










 


DE




N










 


DF




N










 


DG




N










 


DH




N










 


DI




N










 


DJ




N










 


DK




N










 


DL




N










 


DM




N










 


DN




N










 


DO




N










 


DP




N










 


DQ




N
CH3






 


DR




N
CH3






 


DS




N
CH3






 


DT




N
CH3






 


DU




N
CH3






 


DV




N

















The compounds of Formula (IG) could be prepared as described below.




The skilled practitioner will recognize that the various embodiments of the compounds of Formula (IG) disclosed in Table 6 could be prepared by analogous methods using appropriately substituted starting materials, or by appropriate modification e.g. of an amino-functional compound (e.g., compound DP could be prepared by di-alkylation of compound CZ).
Assay Examples
Small molecule chemokine receptor modulation, agonism or antagonism, can be mediated by direct binding to the receptor affecting the signaling and chemotatic effects of the natural ligand for its receptor. In addition modulation can be obtained by interaction of the small molecule with effectors of the particular chemokine receptor pathway. Small molecules can also affect CXCR4 homodimerization (Rodriguez-Frade, et al., J. Cell. Biol. 1999; Mellado et al., Annual Review of Immunology 2001; Toth et al., J. Pharm. and Exp. Ther. 2004; Wang et al., Mol. Cancer. Ther. 2006), heterodimerization with CCR2 (Percherancier, et al. JBC 2005, Sohy et al. JBC 2007) or CCR5 (Babcock, et al., JBC 2003) or CXCR7 (Sierro et al., PNAS 2007), delta opioid receptor (DOR) (Pello et al European J of 1 mm. 2008, Hereld and Jin European J. of 1 mm. 2008) or a T cell receptor (Kumar et al., Immunity 2006). Modulation of the SDF-1/CXCR4 pathway can also be accomplished by targeting the GPR54/KISS receptor (Navenot et al., Cancer Res. 2005), cannabanoid receptor 2 (CB2R) (Coopman et al., International Immunopharmacology 2007), ZAP-70 tyrosine kinase (Ottoson et al., J. Immunology 2001) or sphingosine 1-phosphate receptors (Yopp et al., J. Immunology 2005).
Example 28
Screening by Functional Calcium Mobilization Assay
Functional modulation of CXCR4 was determined by calcium mobilization assay using leukemic lymphoid CEM cells, which naturally express high levels of CXCR4. Generally, the assay is carried out as follows: Cells are grown in vitro to confluency. On the day of the assay, cells are removed from the incubator, culture medium removed and replaced with a calcium sensitive dye (Calcium3 assay kit; Molecular Devices, Sunnyvale, Calif.). The cells are allowed to dye load for 45-60 min at 37° C. in the cell culture incubator and then allowed to equilibrate to room temperature for no more than 15 min before assay.
Compound plates were generated containing up to 3% dimethyl sulfoxide in media kept at room temperature. Test compounds were added to the cells at a 1:3 dilution, and calcium mobilization was measured using a Flex Station fluorescence imager (Molecular Devices). This first read was used to determine direct agonist activation of the CXCR4 receptor by the test compounds. Approximately 45 min after the test compound was added, cells were challenged with SDF-1α at EC80-EC90 concentration and calcium mobilization measured using a Flex Station fluorescence imager. Full dose response curves were generated daily for SDF-1α to calculate IC50 concentrations. The IC50 values are reported. In cases, where one was not determined, a percent inhibition at the 1 μM concentration was extrapolated.
The compounds of the invention generally have an IC50 value below 100 micromolar for inhibition of calcium mobilization induced by SDF-1a. Results for specified compounds are shown in the Table 7.








TABLE 7






Ca Flux IC50 (nM) or


Compound
% inhibition at 1 μM











142 nM 


 






3,200 nM  


 






1,666 nM  


 






5,100 nM  


 






45% 


 






27% 


 






0%


 






4%


 






0%


 






0%


 






6%


 






565 nM 


 






0%


 






729 nM 


 






168 nM 


 






38 nM


 






1,720 nM  


 






55% 


 






1%


 






20% 


 






0%


 






49% 


 






9,999 nM  


 






69 nM


 






10 nM


 






5%


 






18 nM


 






1,930 nM  


 






27% 


 






1,955 nM  


 






22 nM


 






699 nM 


 






39% 


 






451 nM 


 






19% 


 






0%


 






23% 


 






18% 


 






87 nM


 






0%


 






0%


 






30 nM


 






1,340 nM  


 






1,180 nM  


 






100 nM 


 






2,810 nM  


 






 1 nM


 






79 nM


 






1,970 nM  


 






57 nM


 






0%


 






0%


 






331 nM 


 






0%


 






864 nM 


 






22 nM


 






0%


 






949 nM 


 






0%


 






6,671 nM  


 






0%


 






15 nM


 






0%


 






0%


 






663 nM 


 






110 nM 


 






20% 


 






949 nM 


 






1,440 nM  


 






15 nM


 






32% 


 






30 nM


 






3,370 nM  


 






217 nM 


 






44 nM


 






4,390 nM  


 






329 nM 


 






1,046 nM  


 






142 nM 


 






 9 nM


 






1,810 nM  


 






0%


 






 4 nM


 






0%


 






0%


 






8,890 nM  


 






11 nM


 






31 nM


 






1,640 nM  


 






59 nM


 






123 nM 


 






0%


 






0%


 






3,730 nM  


 






21 nM


 






120 nM 


 






6,650 nM  


 






997 nM 


 






0%


 






135 nM 


 






63 nM


 






481 nM 


 






212 nM 


 






9,585 nM  


 






206 nM 


 






24 nM


 






56 nM


 






8,393 nM  


 






61 nM


 






0%


 






3,597 nM  


 






17 nM


 






176 nM 


 






21 nM


 






8,650 nM  


 






628 nM 


 






1,398 nM  


 






2,830 nM  


 






13 nM


 






0%


 






 4 nM


 






78 nM


 






47 nM


 






0%


 






0%


 






8,720 nM  


 






26 nM


 






270 nM 


 






405 nM 


 






152 nM 


 






138 nM 


 






124 nM 


 






583 nM 


 






44 nM


 






30 nM


 






2,150 nM  


 






1,260 nM  


 






20 nM


 






44 nM


 






 9 nM


 






 5 nM


 






0%


 






20 nM


 






 4 nM


 






14 nM


 






49 nM


 






 1 nM


 






0%


 






423 nM 









Example 29
Testing Inhibition of CXCR4 Pathway by Functional Morphological SDF-1a Induced Changes
This assay measures the change in impedance that occurs when cells are stimulated with SDF-1a. Changes in shape and cytoskeleton result in a change of impedance that is dependent on the activation of the CXCR4 receptor. This assay is contracted to MDS Pharma Services and performed as described in http://discoverv.mdsps.com/Catalog/Services/Screening/CellKey/AssayDetails.aspx?id=7 (Assay 930070). Briefly, human HeLa cells expressing endogenous CXCR4 are grown in vitro and receptor activation in live cells is measured using cellular dielectric spectroscopy (CDS). EC50 is defined as a greater than 50% change in cellular impedance relative to the EC100 (10 nM SDF-1a) response. IC50 is defined as a greater than 50% inhibition of the cellular impedance change induced by an EC80 concentration of SDF-1a incubation at 30 minutes.
The compounds of the invention generally have an IC50 value below 100,000 nM the cellular impedance change induced by an EC80 concentration of SDF-1α incubation at 30 minutes. Results for specified compounds are shown in the Table 8.








TABLE 8






Antagonist


Compound
IC50 (nM)




















5.7


 






5.5


 






5.6


 






5.8


 






7.3


 






7.7


 






8.1


 






8.3


 






9.3


 






9.9


 






11


 






12


 






12


 






12


 






15


 






26









Example 30
Test Compound Activity Against HIV Strains
A selected set of compounds are tested for their ability to inhibit the cellular entry of T-tropic HIV. The assay for this inhibition is carried out on a contractual basis at Monogram Biosciences, Inc. using their well established PhenoscreenTM assay. Briefly, HIV strains of interest are tagged with a luciferase indicator gene to create an appropriate test vector. The test vector is amplified through transfection and the resulting virus is incubated in the presence of target host cells where intracellular florescence activity then becomes a measure of infection. Amplified virus is exposed to target host cells in the presence of a range of test drug concentrations to determine IC50 measurements of entry inhibition. A modification of this test is further reapplied as a novel drug assay used in partnership with a number of pharmaceutical companies to test the effectiveness of novel entry inhibitors that target specific chemokines. It can used to detect activity against T-tropic, M-tropic, and dual-tropic viruses and Monogram Biosciences has a large bank of over 10,000 different virus strains to ultimately assess the range of applicability of our chemokine modulators. Certain compounds are tested to establish efficacy in a number of viral strains.
The compounds of the invention generally have an IC50 value for viral entry inhibition in the one of the above HIV viral strains of interest of less than or equal to 100 μM. For example, compounds A, B, Z, AB, AY, and BP have IC50 values of less than or equal to 10 μM.
In addition, compounds are tested in an HIV replication assay carried out by Southern Research Institute. The primary assay screens compounds in a microtiter assay which measures the ability of selected compounds to inhibit HIV-induced cell killing as well as the toxicity of the test compounds to host cells. Quantitation is performed spectrophotometrically using the tetrazolium dye MTS (Cell Titer; Promega) which is converted to a soluble, colored formazan product by mitochondrial enzymes present in metabolically active cells at six days post-infection. The basic assay involves infection of CEM-SS cells with virus in the presence of the test compound. Data are analyzed using a statistical software program developed at Southern and efficacy and toxicity endpoints are determined, as well as selectivity indices. A follow-up assay is performed using in fresh human peripheral blood lymphocytes and monocyte-macrophages infected with low passage primary virus isolates. Blood is obtained from the American Red Cross (screened for HIV and HBV) and mononuclear cells are isolated by Ficoll-hypaque centrifugation. The following are given as examples (and should not be taken as an exhaustive list) of virus strains tested: 92UG029 (X4), 92UG046 (X4), 93UG065 (X4), CMU02 (X4), 91US005 (R5) and 92UG001 (dual X4/R5), the co-receptor used by each given strain is described in paranthesis.
The compounds of the invention generally have an IC50 value for viral entry inhibition in the one of the above HIV viral strains of interest of less than or equal to 100 μM and an antiviral index>10.
Example 31
Screening for CXCR7 Activity
CXCR7 modulation activity was accessed using PathHunterTM β-Arrestin GPCR Assay Pharmacology from DiscoveRx using the protocol recommended by the manufacture for their CXCR7 β-Arrestin cell line. The compounds of the invention generally have an IC50 value below 100 micromolar for CXCR7 modulation activity using this assay.
Example 32
Screening by Competition Assay Using Radiolabelled SDF-1
For radioligand binding competition test of CXCR4 or CXCR7, the following components are mixed in the wells of a 96 well plate (Master Block, Greiner, 786201) up to 100 μL assay buffer (50 mM HEPES; 5 mM MgCl2; 1 mM CaCl2, 250 mM Sucrose, 100 mM NaCl and 0.5% BSA), compounds to be tested or 200-fold excess of cold ligand for non specific binding determination (SDF1-α R&D, 350-NS), radioligand [125I]-SDF-1α (PKI NEX346, 2200 Ci/mmol, diluted in assay buffer to give 0.03 nM) and 1 μg membrane extracts. The plate is incubated during 30 min at 37° C. in a water bath, filtered over GF/B filters (presoaked in 0.5% PEI for 1 h at room temperature) with a Filtermate Harvester (Perkin Elmer), and washed 6 times with 0.5 mL of ice cold filtration buffer (50 mM HEPES; 5 mM MgCl2; 1 mM CaCl2, 250 mM Sucrose, 0.5 M NaCl and 0.5% BSA). Following addition of 50 μL of Microscint 20 (Packard), and incubation during 15 min. on an orbital shaker, the plates are counted with a TopCountTM for 1 min/well.
The compounds of the invention generally have an IC50 value below 100 micromolar for competitive binding versus CXCR4 or CXCR7 activity using this assay.
Example 33
Results of Functional Calcium Mobilization Assay, Cytotoxicity and hERG Binding Assessments for Selected Compounds
Functional calcium mobilization assay was conducted as described in Example 27.
Cytotoxicity of the compounds was assessed by the following assay. CCRF-CEM cells in media (RPMI 1640, 15% FBS, Pen/Strep., 1% non-essential amino acids) are incubated with various concentrations of test compound for 6 days undisturbed. Cell number relative to no compound controls is determined using either Serotec's “Alamar blue” per manufacturers protocol or Promega's “Cell Titer 96 Aqueous One Solution Cell Proliferation Assay” kit per manufacturers protocol. The number cells not surviving after test compound administration is determined for each concentration tested and the EC50 (concentration of compound in which 50% of the cells survive) is determined.
The in vitro effects of the selected compounds on the hERG (human ether-à-go-go-related gene) potassium channel current (a surrogate for IKr, the rapidly activating, delayed rectifier cardiac potassium current) expressed in mammalian cells were evaluated at room temperature using the PatchXpress 7000A (Molecular Devices), an automatic parallel patch clamp system. Each compound was evaluated at varying concentrations in duplicate up to 10 μM, and the duration of exposure to each test article concentration was 5 minutes. For select compounds, the IC50 values for compounds were determined if the concentration in which 50% channel block was observed was <10 μM. For <50% channel block at the 10 μM concentration, an % inhibition at 10 μM is listed. All other compounds have an % inhibition and concentration listed.
A summary of the results is shown in Table 9.










TABLE 9






Ca Flux
Cytotoxicity
hERG


Compound
IC50
EC50
%/IC50











12 nM
30 μM
42% at 10 μM


 






22 nM
30 μM
2.7 μM


 






126 nM 
30 μM
16% at 10 μM


 






16 nM
30 μM
4.1 μM


 






30 nM
50 μM
39% at 10 μM


 






44 nM
30 μM
25% at 10 μM


 






37 nM
0.6 μM 
26% at 1 μM


 






123 nM 
5.3 μM 
18% at 1 μM


 






 7 nM
6.4 μM 
46% at 10 μM


 






113 nM 
>10 μM 
20% at 10 μM


 






55 nM
>10 μM 
45% at 10 μM


 






24 nM
—
3.1 μM


 






56 nM
—
2.4 μM


 






18 nM
—
1.8 μM









Various references have been cited herein, each of which is incorporated herein by reference in its entirety.

## Claims

1. A compound of formula I:



or a pharmaceutically acceptable salt, tautomer, stereoisomer, and/or ester thereof, wherein:




M is H or -G-L3-Z;
E is




L1, L2, and L3 are each independently selected from the group consisting of a covalent bond, substituted or unsubstituted alkylene, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted carbocycyl, and substituted or unsubstituted heterocyclyl;
X, Y and Z are independently H, NRaRb, —ORc, halogen, CF3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, acyl, carboxy, optionally substituted alkoxycarbonyl, optionally substituted aminocarbonyl, optionally substituted alkyl- or dialkylaminocarbonyl, cyano, optionally substituted heterocyclylacyl, optionally substituted carbocyclylacyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl;
Ra and Rb are each independently selected from the group consisting of H, substituted or unsubstituted alkyl, aldiminyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl, and substituted or unsubstituted heterocyclyl; or
Ra and Rb, together with the nitrogen atom to which they are shown attached form a substituted or unsubstituted heterocyclyl;
Rc is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted carbocyclylalkyl, substituted or unsubstituted heterocyclylalkyl,
Q is N;
G is selected from the group consisting of a covalent bond, alkylene, —C(O)—, —C(O)—O—, and —C(O)—NRd—;
Rd is selected from the group consisting of H, alkyl, and substituted or substituted arylalkyl;
R3 and R4, taken together with the carbon atoms to which they are shown attached, form a substituted or unsubstituted carbocyclyl or a substituted or unsubstituted heterocyclyl;
A and B are each independently one or more substituents selected from the group consisting of H, alkyl, halo, substituted or unsubstituted amino, cyano, nitro, haloalkyl, hydroxyl, and alkoxyl;
with the proviso that only one of M, and L1X is H.

2. The compound of claim 1 wherein the compound is of Formula IA-1 or IA-3:



or a pharmaceutically acceptable salt, tautomer, stereoisomer, and/or ester thereof.

3. The compound of claim 2, wherein:
R1 is hydrogen, alkyl, halogen, alkoxy, acyl, alkoxycarbonyl or CF3;
R2 is hydrogen, alkyl, heteroalkyl, hydroxyalkyl, alkoxyalkyl, carbocyclyl, or heterocyclyl; and
L1 is alkylene and X is optionally substituted carbocyclyl, optionally substituted heterocyclyl, or NRaRb; or L1-X is

4. The compound of claim 2, wherein the compound has the Formula IA-2S:




wherein,
L1 and L2 are independently a bond or alkylene; Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl, triazolyl, tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3; and
X is optionally substituted carbocyclyl, optionally substituted heterocyclyl, or NRaRb; or L1-X is

5. The compound of claim 4, wherein:
Q is nitrogen;
L2 is a bond or alkylene;
Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl, tetrazolyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, cyano, halogen or CF3; and
L1-X is

6. The compound of claim 2, wherein the compound has the Formula IA-4S:




wherein,
L1 and L2 are independently a bond or alkylene; Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl, triazolyl, tetrazolyl, acyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, heterocyclylacyl, cyano, halogen or CF3; and
X is optionally substituted carbocyclyl, optionally substituted heterocyclyl, or NRaRb; or L1-X is

7. The compound of claim 6, wherein:
Q is nitrogen;
L2 is a bond or alkylene;
Y is H, NRaRb, —ORc, carbocyclyl, heterocyclyl, tetrazolyl, alkoxycarbonyl, aminocarbonyl, alkyl- or dialkylaminocarbonyl, cyano, halogen or CF3; and
L1-X is

8. The compound of any one of claim 1 or 2 wherein:
L1-X is

9. The compound of claim 1 wherein the compound is:
















































or a pharmaceutical acceptable salt, tautomer, stereoisomer, and/or ester thereof.

10. A pharmaceutical composition comprising a compound of claim 1, 2, or 9 or a pharmaceutically acceptable salt, tautomer, stereoisomer, and/or ester thereof, and a pharmaceutically acceptable carrier.

11. The pharmaceutical composition of claim 10, further comprising at least one additional pharmaceutically active compound.