Set # Name Size (Towne) Forward Reverse Probe Author Date Citation

TRL / IRL

1 TRL11, IRL11 ? ? ? ? ? ? ? ? GTACACGCACGCTGGTTACC GTAGAAAGCCTCGACATCGC Markoulatos 2001 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2001, p. 4426–4432

UL54 / Polymerase

2 UL75 T A P R I M B 218 ACCCGTTACGTGACGGTCAA TTGTCGGCGTTTTCTCCAAA Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

3 UL75 T A P O I M B 268 TACAACAAACCGATTGCCGC CGGCGATGAAGATCTTGAGG Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

4 Pol outer A P O M 293 TGGCCGTGTTCGACTTTGC GAGCGCCATCTGTTCCTTG Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

5 Pol inner T A P O R M 161 CAGCCTCTACCCTTCCATCA GCACCGAGACGCGCACCGAA Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

6 UL54 A P 362 GCCTATCGGTGTCGCTGTACTC GCGAGGTGTCATGTTCGACG Weinberg 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1998, p. 3382–3384

7 CMV_Pol A P O R I M B 61 GCTGACGCGTTTGGTCATC ACGATTCACGGAGCACCAG TCGGCGGATCACCACGTTCG Sanchez 2002 JOURNAL OF CLINICAL MICROBIOLOGY, July 2002, p. 2381–2386

8 Pol A P O R 133 GCCGATCGTAAAGAGATGAAGAC CTCGTGCGTGTGCTACGAGA VIC-AGTGCAGCCCCGACCATCGTTC van Doornum 2003 JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 2003, p. 576–580

9 Pol 66 CATGCGCGAGTGTCAAGAC ACTTTGAGYGCCATCTGTTCCT TGCGCCGTATGCTGCTCCACATA Yun 2003 Journal of Virological Methods Volume 110, Issue 1, 9 June 2003, Pages 73-79

9 CATGCGCGAGTGTCAAGAC ACTTTGAGYGCCATCTGTTCCT TGCGCCGTATGCTCGTCCACATA Herrmann 2004 JOURNAL OF CLINICAL MICROBIOLOGY, May 2004, p. 1909–1914

9 A P O I M B TGCGCCGTATGCTGCTCGACA Ross Haynes Correction based on GenBank AD169 (X17403) sequence data

10 UL54 A P 213 GTAGCTGGCATTGCGATTGGT TCCAACACCCACAGTACCCGT ACGATAGAGCCAGGTGCTGCCGC-X Ibrahim 2005 Journal of Clinical Virology 32 (2005) 29–32

CTGTGCCACTGATCCTTGACCG-P

11 CP1 T A P O R I M B 72 GGCCGTTACTGTCTGCAGGA GGCCTCGTAGTGAAAATTAATGGT CCGTATTGGTGCGCGATCTGTTCAA Sassenscheidt 2006 Journal of Virological Methods Volume 138 (2006) 40-48

12 Pol UL54 T A P O R I M B 281 GGACCTATTCGTTTTCACACCTAC GTGACAGACACGGCGTATGG CAGTATCTGCGTCAACGTTTTCGGGCAG-F Englemann 2008 Journal of Medical Virology 80:467–477

640-GCAGCTACTTTTACTGTGAGTACAGCGACAC-p

13 UL54 A 70 CGCAGTCTACCTCGATATCACAA TGCTCCGTGAATCGTTACGA FAM-CCCTGCTGCCGCCA-MGB Sanghavi 2008 Journal of Clinical Virology 42 (2008) 335-342

UL55&56 / gB

14 gB-B A P 320 TACCCCTATCGCGTGTGTTC CCTCCTATAACGCGGCTGTA Bai 1997 Clinical Chemistry 43:10 p1843-1849

14 TACCCCTATCGCGTGTGTTC CCTCCTATAACGCGGCTGTA Pang 2003 JOURNAL OF CLINICAL MICROBIOLOGY, July 2003, p. 3167–3174

15 UL54 T A P O R I M B 254 TACCCCTATCGCGTGTGTTC ATAGGAGGCGCCACGTATTC Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

15 gB-A TACCCCTATCGCGTGTGTTC ATAGGAGGCGCCACGTATTC Bai 1997 Clinical Chemistry 43:10 p1843-1849

15 gB-A T A P O R I M B Bai 1997 Bai 1997 TTGCTGCCCAGCAGATAAGTGGTG Li 2003 Journal of Clinical Microbiology Vol: 41(1) p 187-191

15 gB-A Bai 1997 Bai 1997 CGTTTCGTCGTAGCTACGCRTACAT-fluorescein Schaade 2000 JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 2000, p. 4006–4009

T A P O R I M B CGTTTCGTCGTAGCTACGCTTACAT Ross Haynes Corrected based on AD169, Towne, Merlin, etc

LC-Red 640-ACACCACTTATCTYCTGGGCAGC-phosphate

T A P O R I M B ACACCACTTATCTGCTGGGCAGC Ross Haynes Corrected based on AD169, Towne, Merlin, etc

15 gB-A TACCCCTATCGCGTGTGTTC ATAGGAGGCGCCACGTATTCT Schaade 2000 Pang 2003 JOURNAL OF CLINICAL MICROBIOLOGY, July 2003, p. 3167–3174

ATAGGAGGCGCCACGTATTC Ross Haynes Corrected based on Bai 1997 sequence data

16 gB outer T A P 150 TGAAGGAATCGCCAGGACGC TGAGGCTGGGAAGCTGACAT Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

17 gB inner A P O R I M B 94 ACGACCCGTGGTCATCTTTA GCGGTGGTTGCCCAACAGGA Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

18 gB-up, -down, -probe A P M B 62 GCCCAAGACATCACCCATG CCATTCTCTCGGCCATTTACA CAAACCGATTGCCGCGCGTTT Yun 2000 Transplantation:Vol 69 p1733-1736

19 Outer UL54 A P R M B 150 GAGGACAACGAAATCCTGTTGGGCA GTCGACGGTGGAGATACTGCTGAGG Jones 2000 JOURNAL OF CLINICAL MICROBIOLOGY, Jan. 2000, p. 1–6

20 Inner UL54 T A P O R I M B 100 TCAATCATGCGTTTGAAGAGGTA ACCACCGCACTGAGGAATGTCAG Jones 2000 JOURNAL OF CLINICAL MICROBIOLOGY, Jan. 2000, p. 1–6

21 GlycoB (TaqMan) A P O I M B 116 AAGTACCCCTATCGCGTGTG ATGATGCCCTCRTCCARGTC TGGCCCAGGGTACGGATCTTATTCG Jebbink 2003 Journal of Molecular Diagnostics, Vol. 5, No. 1, February 2003

22 GlycoB (Mol Becon) A P M B 116 AAGTACCCCTATCGCGTGTG ATGATGCCCTCRTCCARGTC CGTCGAGTTCTATGGCCCAGGGTACGGTCGACG Jebbink 2003 Journal of Molecular Diagnostics, Vol. 5, No. 1, February 2003

23* UL55 A P M B 66 TGGGCGAGGACAACGAA TGAGGCTGGGAAGCTGACAT TGGGCAACCACCGCACTGAGG Boeckh 2004 Journal of Clinical Microbiology Vol: 42(3) p 1142-1148

24 gB A P R M B 138 AAAGAGCTGCGTTCCAGCAA GAGGTCGTCCAGACCCTTGA [FAM]CATGCGCGAATTCAACTCGTACAAGC[TAMRA] Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

UL65

25 UL65 ? ? ? ? ? ? ? ? 124 GCGGTAAGACGGGCAAATAC GGCGTCGAGATGTTCGTAGAG [FAM]CACCATCGACACCACACCCTCATGA[TAMRA] Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

UL75

26 UL75 ? ? ? ? ? ? ? ? 226 TGGACGAGGCTGCCCATGAGG TGGACCTGGCCAAACGAGCCC CCAGGCGTACGAGGCTGGTGATGTCGGTGA Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

27 UL75 ? ? ? ? ? ? ? ? 264 CCCTGTTCCAGTAGCGGGCGA GACGGTGGTGGAGACAGGGT Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

28 UL75 ? ? ? ? ? ? ? ? 231 ACCCATGCTGCTTATCTTCGG GCGTAACGGTGAAAGCTGTTACGTA Allen 1995 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1995, p. 725–728

UL83 / pp65

29 UL83 T A R I M B 283 GTCAGCGTTCGTGTTTCCCA GGGACACAACACCGTAAAGC FAM-CCCGCAACCCGCAACCCTTCATG-TAMRA Gault 2001 Journal of Clinical Microbiology Vol: 39(2) p 772-775

29 UL83 GTCAGCGTTCGTGTTTCCCA GGGACACAACACCGTAAAGC FAM-CCCGCAACCCGCAACCCTTCATG-TAMRA Gouarin 2002 Journal of Clinical Microbiology Vol: 40(5) p 1767-1772

30 UL83 T A R I B 279 GACACAACACCGTAAAGC CAGCGTTCGTGTTTCC TTGCGGGTCATCGTCAGGTCCTC-FL Stocher & Berg (2002) JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2002, p. 4547–4553

LC-Red-705-TCCACGTCAGAGCCCAGCGTGA-p

31 UL83 T A P R I M B 159 GCAGCCACGGGATCGTACT GGCTTTTACCTCACACGAGCATT FAM-CGCGAGACCGTGGAACTGCG-TAMRA Griscelli 2001 Journal of Clinical Microbiology Vol: 39(12) p 4362-4369

32 plasmid standard T A P R I M B 412 CGACGACGACGTCTGGACCAG CTGCCATACGCCTTCCAATTCG Piiparinen 2004 Journal of Clinical Virology 30 (2004) 258–266

33 UL83 T A P O R I M B 57 TCGCGCCCGAAGAGG CGGCCGGATTGTGGATT CACCGACGAGGATTCCGACAACG Piiparinen 2004 Journal of Clinical Virology 30 (2004) 258–266

UL122 - 126 / MIE

34 MIE outer A 242 TGAGGATAAGCGGGAGATGT ACTGAGGCAAGTTCTGCAGT Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

35 MIE inner T A M 146 AGCTGCATGATGTGAGCAAG GAAGGCTGAGTTCTTGGTAA Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

36 MIE outer T A 256 TTGTACCTGAGGATAAGCG GGGGAGCACTGAGGCAAGT Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

37 MIE inner T A P O R I M B 192 CGGGAGATGTGGATGGCTTG GCCATTGGTGGTCTTAGGGA Wirgart 1998 JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1998, p. 3662–3669

38 MIE T A 427 GCACCATCCTCCTCTTCCT GGCCTCTGATAACCAAGCC CCTCCTGAGCACCCTCCTCCTCTTCC Nitsche 1999 Clinical Chemistry 45:11 p1932–1937

38 MIE T A 215 GCACCATCCTCCTCTTCCT GATTCTCTGGTGTCACCCC CCTCCTGAGCACCCTCCTCCTCTTCC (In house reverse)

38 T A GCACCATCCTCCTCTTCCT GGCCTCTGATAACCAAGCC CCTCCCGCTCCTCCTGAGC-FL Nitsche 1999 Clinical Chemistry 45:11 p1932–1937

LC-CCCTCCTCCTCTTCCTCATCACTC-p

38 T A GCACCATCCTCCTCTTCCT GGCCTCTGATAACCAAGCC CACAGTGTCCTCCCGCTCCTC-FL Nitsche 1999 Clinical Chemistry 45:11 p1932–1937

LC-TGAGCACCCTCCTCCTCTTCCT-p

38 T A GCACCATCCTCCTCTTCCT GGCCTCTGATAACCAAGCC TGATGAGGAAGAGGAGGAGGGTGCT-FL Nitsche 1999 Clinical Chemistry 45:11 p1932–1937

LC-CTCAGGAGGAGCGGGAGGACAC-p

39 MIEA outer T A 432 AGCACCATCCTCCTCTTCCTCTGG AAGCGGCCTCTGATAACCAAGCC Drago 2004 BMC Infectious Diseases 2004, 4:55

40 MIEA inner T A P O R I M B 110 AGTGTGGATGACCTACGGGCCATCG GGTGACACCAGAGAATCAGAGGAGC Drago 2004 BMC Infectious Diseases 2004, 4:55

41 Outer MIE T A 434 CCAAGCGGCCTCTGATAACCAAGCC CAGCACCATCCTCCTCTTCCTCTGG Tokimatsu 1995 Chest 1995; 107:1024-27

41 Outer MIE CCAAGCGGCCTCTGATAACCAAGCC CAGCACCATCCTCCTCTTCTCTGG Ikewaki 2003 JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2003, p. 4382–4387

41 CAGCACCATCCTCCTCTTCCTCTGG Ross Haynes Correction based on Tokimatsu 1995

42 Inner MIE 162 CCACCCGTGGTGCCAGCTCC CCCGCTCCTCCTGAGCACCC Tokimatsu 1995 Chest 1995; 107:1024-27

42 Inner MIE CCACCCGTGGTGCCAGCTCC CCCGCTCCTCCTGAGCACCC Ikewaki 2003 JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2003, p. 4382–4387

42 Corrected For Primer A CCACCGCTGGTGTCAGCTCC Chou 1992 JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 1992, p. 2307-2310

42 T M CCACCGCTGGTGCCAGCTCC Ross Haynes Correction based on Towne (AY315197) sequence data

43 A T A 435 CAGAGGAAGAGGAGGATGGTGCTG CCAAGCGGCCTCTGATAACCAAGCC CTACGGGCCATCGCCGAGGAGT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

44 B T A 435 CAGAGGAAGAGGAGGATGGTGCTG CCAAGCGGCCTCTGATAACCAAGCC CCTCCTGAGCACCCTCCTCCTCTTCC Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

45 C T A P O R I B 435 CAGAGGAAGAGGAGGATGGTGCTG CCAAGCGGCCTCTGATAACCAAGCC CCGATCCTCTGAGAGTCTGCTCTCCT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

46 D T A 427 GCACCATCCTCCTCTTCC GGCCTCTGATAACCAAGCC CTACGGGCCATCGCCGAGGAGT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

47 E T A 427 GCACCATCCTCCTCTTCC GGCCTCTGATAACCAAGCC CCTCCTGAGCACCCTCCTCCTCTTCC Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

48 F T A P O R I B 427 GCACCATCCTCCTCTTCC GGCCTCTGATAACCAAGCC CCGATCCTCTGAGAGTCTGCTCTCCT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

49 G A 227 GCACCATCCTCCTCTTCC GTCAGCTCCTCTGATTCTC CCTCCTGAGCACCCTCCTCCTCTTCC Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

50 H T A 96 GGCTCAGACTTGACAGACACA GAACAGTGATCAGGAAGAAAGTGA CCTCCTGAGCACCCTCCTCCTCTTCC Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

51 J T A 173 GGGTGACACCAGAGAATCAGAG TCTGCATGAAGGTCTTTGCC CTACGGGCCATCGCCGAGGAGT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

52 K T A P O R I B 173 GGGTGACACCAGAGAATCAGAG TCTGCATGAAGGTCTTTGCC CCGATCCTCTGAGAGTCTGCTCTCCT Nitsche 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2734–2737

53 UL122 T A M 201 GACTAGTGTGATGCTGGCCAAG GCTACAATAGCCTCTTCCTCATCTG AGCCTGAGGTTATCAGTGTAATGAAGCGCC Tanaka 2000 Journal of Medical Virology 60:455–462 (2000)

53 RQ GACTAGTGTGATGCTGGCCAAG GCTACAATAGCCTCTTCCTCATCTG AGCCTGAGGTTATCAGTGTAATGAAGCGCC Lengerova 2007 JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2007, p. 1042–1044

Matches Genome
54 IE plasmid T A M 876 TTTTTAGCACGGGCCTTAGC CACGATGGAGTCCTCTGCCA Yun 2000 Transplantation:Vol 69 p1733-1736

55 IE4-up, -down, -probe T A M 77 TTTTTAGCACGGGCCTTAGC AAGGAGCTGCATGATGTGAGC TGCAGTGCACCCCCCAACTTGTT Yun 2000 Transplantation:Vol 69 p1733-1736

56 MIE 168 TTGCTCACATCATGCAGCTCCT AAGGCATTCTGCAAACATCCT Funato 2001 Journal of Viral Hepatitis, 2001, 8, 217-222

T A M AGGATGTTTGCAGAATGCCTT Ross Haynes Correction - reverse complement of published reverse primer 

57 MIE 213 ACGACGTTCCTGCAGACTAT AAGAGAAAGATGGACCCTGA Funato 2001 Journal of Viral Hepatitis, 2001, 8, 217-222

T A P O R I M B ATAGTCTGCAGGAACGTCGT Ross Haynes Correction - reverse complement of published forward primer 

58 Long MIE T A P O R I M B 578 ACAAGGTGCTCACGCACATTGATC CACTGGCTCAGACTTGACAGACAC TGAAGGTCTTTGCCCAGTACATTCT Boom 2002 Journal of Clinical Microbiology Vol: 40(11) p 4105-4113

59 Short MIE T A P O R I M B 138 CCAAGCGGCCTCTGATAACCAA GGTCATCCACACTAGGAGAGCAGAC TGAAGGTCTTTGCCCAGTACATTCT Boom 2002 Journal of Clinical Microbiology Vol: 40(11) p 4105-4113

60 IE T A P O R I B 127 CAAGCGGCCTCTGATAACCA ACTAGGAGAGCAGACTCTCAGAGGAT FAM-TGCATGAAGGTCTTTGCCCAGTACATTCT-TAMRA Kalpoe 2004 JOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2004, p. 1498–1504

61 IE 369 GGGTGCTGTCCTGCTATGTCTTA CATCACTCTGCTCACTTTCTTCC CGGCCTCTGATAACCAAGCCTG Li 2003 Journal of Clinical Microbiology Vol: 41(1) p 187-191

T A GGGTGCTGTGCTGCTATGTCTTA Ross Haynes correction based on Genbank Towne, AD169 and Merlin sequences

62 MIE T A P O R I B 73 AGCGCCGCATTGAGGA CAGACTCTCAGAGGATCGGCC ATCTGCATGAAGGTCTTTGCCCAGTACATT Leruez-Ville 2003 JOURNAL OF CLINICAL MICROBIOLOGY, May 2003, p. 2040–2046

63 UL125 A P 86 CCAGTGCCCGCAGTTTTTATT ACCGGAGAAGAGCCCATGTC AACATAACGTGGGATCTCCACGCGAAT Boeckh 2004 Journal of Clinical Microbiology Vol: 42(3) p 1142-1148

64 UL126 T A P O R I M B 67 ACCGTCAGATCGCCTGGA GATCGGTCCCGGTGTCTT ACGCCATCCACGCTGTTTTGACCTC Boeckh 2004 Journal of Clinical Microbiology Vol: 42(3) p 1142-1148

65* UL123-exon4 T A P R I M B 84 TCCCGCTTATCCTCRGGTACA TGAGCCTTTCGAGGASATGAA TCTCATACATGCTCTGCATAGTTAGCCCAATACA Boeckh 2004 Journal of Clinical Microbiology Vol: 42(3) p 1142-1148

66 MIE outer 435 CCAAGCGGCCTCTGATAACCAAGCC CAGCACCATCCTCCTCTTCTCTGG Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

T A CAGCACCATCCTCCTCTTCCTCTGG Ross Haynes correction based on Towne and AD169 sequences

P O I M B CAGCACCATCCTCCTCTTCCTCTGA Ross Haynes based on PH-BAC, Toledo, FIX-BAC, Merlin, and TB40/E sequences

67 MIE inner 162 CCACCCGTGGTGCCAGCTCC CCCGCTCCTCCTGAGCACCC Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

T M CCACCGCTGGTGCCAGCTCC Ross Haynes Correction based on Towne and Merlin sequence data

O R I CCACCGCTGGTGCTAGCTCC Ross Haynes Correction based on other sequequnce data

A CCACCGCTGGTGTCAGCTCC Ross Haynes Correction based on AD160 (X17403) sequnce data

68 IE T A P O R I B 94 CAGATTAAGGTTCGAGTGGACATG AGGCGCCAGTGAATTTCTCTT [FAM]TGCGGCATAGAATCAAGGAGCACATG[TAMRA] Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

69 IE 2 exon 5 region 53 GAGCCCGACTTTACCATCCA CAGCCGGCGGTATCGA VIC-ACCGCAACAAGATT-MGBNFQ Boppana 2010 JAMA, April 14, 2010—Vol 303, No. 14, 1375-1382

US7 - 9

70 US7, 8 ? ? ? ? ? ? ? ? GGATCCGCATGGCATTCACGTATGT GAATTCAGTGGATAACCTGCGGCGA Drouet et al. (1995) JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 1995, p. 389–394

GGATCCGCATGGCATTACCGTATGT Corrected in Habbal et al., 2005

71 US9 ? ? ? ? ? ? ? ? GGACGTATCCACCTCAGGTACACA TACGTTACGAAACTGAGCTCCCAC CGTGTTTCACAAACTGCACCAGTACCAC-Fluor Razonable et al. (2001) JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2001, p. 4472–4476

Red640-TAGAGGAATGTCAGGTAGCGTCTCTCCG-Phos

US17

72 US17 A P R I M B 151 GCGTGCTTTTTAGCCTCTGCA AAAAGTTTGTGCCCCAACGGTA [FAM]TGATCGGCGTTATCGCGTTCTTGATC[TAMRA] Machida 2000 JOURNAL OF CLINICAL MICROBIOLOGY, July 2000, p. 2536–2542

72 US17 GCGTGCTTTTTAGCCTCTGCA AAAAGTTTGTGCCCCAACGGTA [FAM]TGATCGGCGTTATCGCGTTCTTGATC[TAMRA] Ikewaki 2003 JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2003, p. 4382–4387

72 US17 GCGTGCTTTTTAGCCTCTGCA AAAAGTTTGTGCCCCAACGGTA [FAM]TGATCGGCGTTATCGCGTTCTTGATC[TAMRA] Ikewaki 2005 Bone Marrow Transplantation (2005) 35, 403–410

73 US17 T A P O I M B 60 CGATCAAGAACGCGATAACG ACCGTCGATGGCAGGTCAT FAM-CGATCACAAACAGCG-MGB Sanghavi 2008 Journal of Clinical Virology 42 (2008) 335-342

* Primers originally a duplex

T = Towne (AY315197.2)

A = AD169 (X17403.1)

P = PH-BAC (AC146904.1) Other resources

O = Toledo (AC146905.1) Errors in Published Sequences of Human Cytomegalovirus Habbal 2005 JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 2005, p. 5408–5409

R = TR-BAC (AC146906.1) Primers and Probes: Do We Need More

I = FIX-BAC (AC146907.1) Quality Control?

M = Merlin (NC_006273.2) Optimization of Human Cytomegalovirus LightCycler Real-Time PCR Habbal 2008 J Infect Developing Countries 2008; 2(5):406-410

B = TB40/E (EF999921.1) Comparative evaluation of published cytomegalovirus primers for Habbal 2009 Journal of Medical Microbiology (2009), 58, 878–883

rapid real-time PCR: which are the most sensitive?