NIST GENOME EDITING WORKSHOP

MAY 3, 2019

NIST, 100 BUREAU DRIVE, GAITHERSBURG, MD 20899

BLDG. 101, GREEN AUDITORIUM

8:00 am Check-in at registration desk

8:30 am Opening Remarks

Eric Lin, Ph.D., Director, Material Measurement Laboratory, NIST

8:40 am Introduction to NIST Genome Editing Consortium

Samantha Maragh, Ph.D., Leader, NIST Genome Editing Program

8:50 am NIH Somatic Cell Genome Editing Program

Mary Perry, Ph.D., Program Leader, NIH Office of Strategic Coordination

9:10 am DARPA Safe Genes and PREPARE Programs

Renee Wegrzyn, Ph.D., Program Manager, DARPA Biological Technologies Office

9:30 am Genome in a Bottle: So you’ve sequenced a genome – how well did you do?

Justin Zook, Ph.D., Leader NIST Genome in a Bottle Consortium

9:50 am NIST Automation Suite and Experimental Design

John Elliott, Ph.D., Leader NIST Cell Systems Science Group

10:10 am BREAK

10:20 am

Large-scale CHANGE-seq Genome-wide Profiling of CRISPR-Cas9 Reveals Effects of 

Sequence, Epigenetic Factors, and Human Genetic Variation on Activity

Shengdar Tsai, Ph.D., Assistant Member, Dept. of Hematology St. Jude Children's Hospital 

10:45 am Challenges in Characterization of Genome Editing

Chris Fuller, Ph.D., VP Informatics, Caribou Biosciences, Inc.

11:10 am Panel Discussion

Mary Perry, Ph.D., Program Leader, NIH

Renee Wegrzyn, Ph.D., Program Manager, DARPA Biological Technologies Office

Justin Zook, Ph.D., Leader NIST Genome in a Bottle Consortium

John Elliott, Ph.D., Leader NIST Cell Systems Science Group

Shengdar Tsai, Ph.D., Assistant Member, Dept. of Hematology St. Jude Children's Hospital

Chris Fuller, Ph.D., VP Informatics, Caribou Biosciences, Inc.

Anna Kwilas, Ph.D., Biologist, FDA Center for Biologics Evaluation and Research (CBER)
NIST Genome Editing Consortium Updates

11:30 am Working Group 1: Specificity

Samantha Maragh, Ph.D., Leader, NIST Genome Editing Program

11:45 am Working Group 2: Data & Metadata

Luca Pinello, Ph.D., Investigator, MGH/Harvard Medical School

12:00 pm Working Group 3: Lexicon

Brett Robb, Ph.D., Scientific Director, RNA & Genome Editing, New England Biolabs

12:10 pm NIST Genome Editing Consortium Q & A 

12:20 pm LUNCH BREAK (lunch for purchase at NIST cafeteria)

 Technology Talks

1:10 pm Using PacBio’s Long, Accurate HiFi Reads in the Genome Editing Pipeline

Andy Larrea, Ph.D., Sr. Manager Field Business Development, Pacific Biosciences

1:30 pm

Multiplexed target enrichment and high-resolution NGS analysis of on- and off-target 

CRISPR-Cas9 editing events via rhAmpSeqTM technology

Garrett Rettig, Ph.D., Director of Molecular Genetics Product Development, IDT 

1:50 pm Safety considerations for delivering medicines by therapeutic genome editing

Roberto Nitsch, Ph.D., Associate Director, Drug Safety and Metabolism, AstraZeneca

2:10 pm Single cell assessment of multiplexed DNA variation using the Tapestri platform

Keith Jones, Ph.D., Senior Vice President of Product Development, Mission Bio

Laurakay Bruhn, Ph.D., Section Manager, Biological Chemistry, Agilent

2:40 pm Closing Remarks

2:45 pm Adjourn

 3:00 pm Shuttle departure from NIST to Hilton Gaithersburg 

3:00 – 4:30 pm Closed NIST Genome Editing Consortium Meeting (Portrait Room)

5:00 pm Shuttle departure from NIST to Hilton Gaithersburg