Chapter 1 - General Information

Chapter 2 - Liability, Warranty and Safety

Chapter 3 - Principles of Operation

Chapter 4 - Touch Panel Operation

Chapter 5 - System Screens

Chapter 6 - Installation Requirements

Chapter 7 - Installation

Chapter 8 - Commissioning the System

Chapter 9 - System Operation

Chapter 10 - Maintenance & Troubleshooting
Edition Information: Edition January 2007 

M.Braun, Inc. 

14 Marin Way • Stratham, NH 03885 

Phone 603-773-9333 • Fax 603-773-0008 

Email: info@mbraun.com 

www.mbraunusa.com 

Revision Information: Revision 002 

Title Operating Instructions – Supplement A 

Technical Consultation: K. Prince, Programming 

T. Daly, Service 

Technical Assistance: K. Prince, Programming 

T. Daly, Service 

Layout: B. Adams, Quality Manager, M.Braun, Inc. 

Publication edited by means of: Adobe Acrobat®, Adobe Photoshop®, Microsoft Word®

Important Notes: This technical documentation is not liable to any 

obligations on the part of the manufacturer. The 

manufacturer M.Braun, Inc. reserves the right for 

technical and optical modifications as well as functional 

modifications on the system or system’s components 

described therein. 

Any duplication of this documentation, even in the 

forms of excerpts, is only permitted after having 

obtained the manufacturer’s information and 

concession. 
C

hapter1

GeneralInformation

1.1Liability....1-1

1.2ContactInformation....1-2
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 1: General Information 

© MBraun, Inc. Edition 1/2007 Page 1-1

CHAPTER 1: GENERAL INFORMATION

1.1 Entries Referring to the System 

This documentation is part of the system: 

Designation / Type: 

Series Number(s): 

Persons In Charge of System: 

Space left for notes on system settings, instructions for maintenance, etc. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 1: General Information 

© MBraun, Inc. Edition 1/2007 Page 1-2

1.2 Contact Information 

M.Braun, Inc. 

14 Marin Way 

Stratham, NH 03885 

Phone: 603-773-9333 

Fax: 603-773-0008 

Email: service@mbraunusa.com 

Internet: www.mbraunusa.com 
Chapter 2

Liability, Warranty

and Safety

2.1 General Information....2-1

2.2 Liability....2-1

2.3 Warranty....2-2

2.4 Operation Guidelines....2-2

2.5 Safety - General Hazards....2-3

2.6 Safety - Mechanical....2-3

2.7 Safety - Electrical....2-4

2.8 Safety - Handling of Electronic Components....2-5

2.9 Safety - Chemicals and Gases....2-5

2.10 Safety - Symbols Used on the System....2-6

2.11 Safety - Emergencies....2-6

2.12 Additional Information....2-7
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-1

CHAPTER 2: LIABILITY, WARRANTY & SAFETY

2.1 General Information 

Prior to installation, initialization and operation of the system, this manual should be read in its 

entirety and positioned in a suitable area near the system to allow for easy reference. Any 

persons charged with the transport, storing, installation, commissioning, operating, maintenance 

and/or service of this system must be familiar with the entire contents of this manual. 

To ensure safe operation of the system and to maintain a safe working environment, the 

information contained within this chapter must be adhered to by all users of the system. Advice 

contained in this chapter is intended to supplement, not supersede, the safety advice given in 

other chapters of this manual and the general safety regulations and guidelines prevailing in the 

user’s workplace. 

In addition to the guidelines and information contained within this manual all internal and local 

health, safety and environmental guidelines should be followed. 

Safety instructions and pertinent information are marked in the following manner: 

Indicates an imminently hazardous situation which, if not avoided, 

will result in death, serious injury or serious damage to the system, 

other equipment or surrounding environment. 

Indicates a potentially hazardous situation which, if not avoided, 

could result in death, serious injury or serious damage to the 

system, other equipment or surrounding environment. 

Indicates a potentially hazardous situation which, if not avoided, 

may result in minor or moderate injury or damage to the system, 

other equipment or surrounding environment. 

Indicates additional advice or recommendations for using the 

system or completing a task. 

Please consider all instructions, in particular safety instructions, in order to achieve safe 

operation of the system. 

2.2 Liability 

The manufacturer will not take any liability of object, personal or secondary damage caused by 

improper use or ignoring of safety instructions as well as caused by the owner’s manual due to 

missing updates after the system or its software have been modified, nor will the manufacturer 

take any liability of damages due to loss of data. In addition, the terms of business that are part 

of the order/contract will apply. 

Our products are continuously modified an improved due to innovation, legal requirements and 

standards. Consequently, the information give in this documentation may not accurately reflect 

every detail of the system actually delivered. Please contact the manufacturer in cases of 

uncertainty. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-2

2.3 Warranty 

We guarantee the equipment as stated in the order/contract. 

This warranty will expire in case of: 

• Interference into or modification of the system without prior consent of the manufacturer; 

• Improper use of the system; 

• Insufficient maintenance of the system; 

• Inappropriate operation of the system; 

• Negligence of correct supply requirements; 

• Application of third-part components to the system without prior consent of the 

manufacturer; 

• Alteration of program or configuration write-ups without manufacturer’s consent. 

This applies to a single unit and multi-unit system types. 

2.4 Operation Guidelines 

MBRAUN glovebox systems are operated using inert gas enabling the user to handle 

substances which are sensitive to oxygen and/or moisture. It is the responsibility of the user to 

follow all local health, safety and environmental guidelines with regards to the handling and 

disposing of substances which may be injurious to health. This also applies to the disposal of 

vacuum pump oil and all components and filtering devices which come in contact with the gas 

flow. 

There is a risk of suffocation when working with high inert gas 

concentrations. 

On request, MBRAUN can recommend a personal measuring instrument that alerts the 

operator to a reduction of oxygen content in the ambient air. 

The following general safety guidelines must be considered when working with inert gas 

concentrations: 

• The selected location should have a “room” volume that is significantly larger than the 

glovebox interior volume. 

• The system should be located in a well ventilated area. This is especially important 

during a purging procedure or when opening an active system (i.e. antechambers, etc.). 

• All exhaust fumes should be vented through an adequate disposal/ventilation system. 

Contact MBRAUN prior to the acquisition of the system if it is not 

possible to adhere to all the above recommendations. This way the 

system can be equipped with additional safety devices. 

• Prior to performing maintenance or service inside an active system, remove one glove to 

allow a slow equalization of the glovebox interior atmosphere with the ambient room air. 

To better avoid the risk of suffocation, it is necessary for the 

glovebox atmosphere to be completely replaced with ambient room 

air prior to servicing the interior of an active glovebox. 

Standard MBRAUN glovebox systems are not designed for the use 

of strongly poisonous or radioactive substances. Use of these types 

of materials must be coordinated with MBRAUN prior to the 

acquisition of a system. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-3

2.5 Safety – General Hazards 

This system has been designed and manufactured considering all relevant safety regulations. 

Improper use or operation by persons not qualified accordingly may result in danger to the: 

• Life and health of the operator; 

• System itself; 

• Surroundings of the user; 

• Performance and efficiency of the system. 

General hazards of the system may arise in the following ways: 

• Mechanical hazard caused by squeezing, shearing and cutting, catching and winding, 

stretching or by freely moving parts; 

• Thrust caused by kinetic energy of moving mass; 

• Sharp corners and edges; 

• Electrical hazard caused by touching live parts (directly or indirectly); 

• Thermal hazard causing burns; 

• Chemical hazard causing poisoning, corrosion and explosion; 

• Toxic hazard due to inhalation of vapours and gases; 

• Gases under pressure; 

• Liquids under pressure; 

• Combination of hazards caused by: 

– faulty installation 

– incorrect loading 

– breakdown of power or media supply 

– breakdown and/or incorrect arrangement of preventive measures 

– combination of escaping media 

• Hazards caused by: 

– human misconduct 

– noise 

– allergies, excitations of mucous membrane, unknown effects caused by media 

– ejection of parts 

– disturbance / malfunction of control system 

– leaking of hoses or pipes 

– combination of atmospheres or vapors 

– fire hazard 

– natural hazards e.g. lightning, flooding, environmental catastrophes etc. 

2.6 Safety – Mechanical 

Freely moving parts may cause squeezing, shearing and cutting, 

catching and winding, stretching of extremities. Extreme caution 

should be taken to avoid touching any moving parts of the system 

during operation. 

When handling materials with mechanical, pneumatic or vacuum systems it is possible that 

materials may be ejected. Extreme caution should be taken to avoid any possible contact with 

the ejected materials without proper protection. 

Only genuine parts supplied by MBRAUN should be used in the operation of the system. These 

parts are constructed in conformance with applicable safety regulations. No liability will be taken 

by MBRAUN in the event of installation of parts manufactured by companies other than 

MBRAUN which may result in additional and unknown hazards. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-4

Simultaneous operation of the system by two or more persons is not recommended as this may 

cause hazards based on misconduct or mutual misunderstanding. In case of the system being 

operated by two or more persons, operation should be conducted in such a way as to ensure 

each individual’s respective task does not influence other tasks in any way. 

Safety covers, panels, panes, windows or doors may not be removed at any time, unless there 

is a need for service. The system may not be opened (i.e. antechambers, etc.) during 

processing or power failures. In the case of any safety deficiencies, the system must be 

decommissioned and the service personnel informed accordingly. During decommissioning 

compliance with all local health, safety and environmental guidelines must be followed. 

2.7 Safety – Electrical 

This system operates under high voltage. Risk of injury caused by high voltages exists anytime 

the system is connected to the power supply, this includes when the system is powered off. 

Capacitors within the system may be charged when the system is switched off and 

disconnected from main power supply. 

Interchange of current bearing wires can result in electrical hazards 

such as shock, involuntary muscle reaction, muscle paralysis, burnt 

tissues and organs, or death. 

Connection to the main power supply must be performed by a qualified electrician according to 

local area guidelines. All neutral and ground wires must be connected accordingly. 

Opening the system or removing parts when the system is powered on, may result in exposure 

to live electrical connectors. Extreme caution should be taken to avoid directly or indirectly 

touching live connectors to avoid possible electric shock. 

Prior to performing any electrical service work on the system, 

ensure the system is powered off and disconnected from the power 

supply. 

Service required while the system is in operation should only be performed only by qualified 

personnel trained in the knowledge and prevention of all potentially dangerous and hazardous 

situations. 

The system must be grounded/earthed at all times. Do not remove or cut off any ground wire for 

the system or its components. In case of insufficient grounding or damaged ground conductor 

ensure the system will be inoperable and secure it against unauthorized or unintentional 

operation. 

Insufficient grounding can cause electrostatic charging of plastic 

parts, hoses or pipes, wiring and/or the system as a whole, which 

could cause solvents and process chemicals to ignite.

Replacement of fuses should be of the same type and current rating. 

Makeshift fuses and/or short circuit fuse holders should never be 

used in the operation of the system. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-5

2.8 Safety – Handling of Electronic Components 

Electrostatic discharges can cause damage to parts. When handling electronic components the 

following precautions should be observed: 

a) Wear a grounded wrist strap or work on a grounded static-dissipating work surface. If 

this not possible touch an adjacent earth ground (i.e. central heaters or water pipes) 

before handling electronic components or printed circuit boards. 

b) Leave electronic components and printed circuit boards in their original packaging 

until final installation. 

c) Handle electronic components by their body or case, avoid touching of leads. 

d) Keep electronic components and printed circuit boards away from such static 

generating materials as vinyl, plastic bags, etc. 

Maintenance and repair work required, but not listed in this manual, 

should only be carried out by MBRAUN service or by persons of 

equivalent qualification. 

2.9 Safety – Chemicals and Gases 

Chemicals used in the system are not supplied by MBRAUN. Chemicals are provided and 

applied by the system user. 

Proper handling of chemicals, corrosives and solvents is the user's responsibility. Materials 

used may be flammable, explosive, toxic. 

Below are some guidelines to refer to when handling chemical substances: 

• Ensure the all relevant Control of Substance Hazardous to Health (COSHH) guidelines 

are followed; 

• Observe relevant safety regulations as well as material safety data sheets (MSDS) and 

additional advice provided by the supplier; 

• Wear proper protective safety masks, gloves and eyewear whenever working with 

chemicals, corrosives or solvents; 

• Mark all containers and supply lines of chemicals (i.e. containers of media and waste) 

with appropriate labels and warning signs; 

• Ensure proper ventilation and exhaustion of vapours; 

• Wear proper breathing protection to avoid the risk of suffocation; 

• Keep away from ignition sources; 

• Do not smoke; 

• Do not ingest food or beverage while working with the chemicals, corrosives or solvents 

to avoid the risk of poisoning. 

Released chemicals may react with each other, leading to 

unwanted and/or unknown substances, which may cause additional 

risks. 

Proper handling of gases is the user's responsibility. Gases used may be flammable, explosive, 

toxic. Below are some guidelines to refer to when handling gaseous materials: 

• Do not inhale the gas to avoid risk of suffocation. 

• Prevent electrostatic charging and beware of ignition sources. 

• Do not smoke. 

When using corrosive, gassing or noxious materials, the safety of 

all employees must be ensured by whatever means necessary. 

Specifically, all employees must be trained in the safe handling of 

the materials to be used. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-6

2.10 Safety – Symbols Used on the System 

The following symbols refer to MBRAUN components and parts. However, components and 

parts of sub-suppliers may show other symbols, not expressly mentioned or referred to in this 

manual. The following caution and command symbols may be seen on the system: 

General hazard Electrical hazard Fire hazard 

Toxic hazard Explosion hazard Pressurised gas hazard 

Wear safety mask Wear safety goggles Wear protective gloves 

The owner of the system is responsible to place adequate danger 

signals and labels in suitable places. This applies in particular to 

signals and labels concerning process chemicals used. Regardless 

of the number of caution symbols and information placed on or 

around the system, all safety instructions of this manual must be 

observed! 

2.11 Safety – Emergencies 

In case of an emergency, please observe the following instructions: 

1. Immediately shutdown the system using the main power switch. 

2. Disconnect the system from all gas supplies. 

3. Refer to the material safety data sheets for information on treating the emergency. 

Contact the appropriate emergency response personnel in the area and/or listed on the 

material safety data sheets. 

Do not disconnect the water supply for systems containing 

components requiring a cooling water source. 

Prior to restarting, the system must be fully checked for safety, contact the MBRAUN Service 

Department after the emergency has been rectified. 

In addition to the information contained in this manual all local 

health, safety and environmental guidelines must be followed. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 2: Liability, Warranty & Safety 

© MBraun, Inc. Edition 1/2007 Page 2-7

2.12 Additional Information 

The system is considered to be unsafe for operation if: 

• there is any visible damage; 

• it fails to perform according to specification; 

• it has been subject to prolonged storage under unfavourable conditions; 

• it has been subjected to severe transport stress. 

If the system meets any or all of the above: 

• make it inoperable; 

• secure it against any unauthorized or unintentional operation; 

• contact the MBRAUN Service Department. 

Do not perform any service or repair of the system or its 

components other than described in this manual. 

Maintenance, repair and service other than described in this manual 

may only be performed by MBRAUN service personnel or properly 

trained/qualified individuals. 
Chapter 3

Principles of Operation

3.1 Description of the System....3-1

3.2 Pressure Control System....3-1

3.3 Purging the System....3-2

3.4 Circulation Mode....3-3

3.5 Regeneration Mode....3-3

3.6 Antechamber Operation....3-4

3.7 Analyzers....3-5
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-1

CHAPTER 3: PRINCIPLES OF OPERATION

3.1 Description of the System 

M.Braun glovebox systems, which are operated using a closed loop circulation method, provide 

the user with a non-reactive atmosphere of <1ppm moisture and oxygen content. The system 

has three major functions: Purging, Circulation and Regeneration. Each of these processes is 

explained in detail in the following sections. For information for operating the system functions, 

refer to Chapter 9 of this manual. 

3.2 Pressure Control System 

General Information 

The system is equipped with a PLC operated pressure control system which starts automatically 

when the system is activated. Upper and lower working pressure parameters, which are set by 

the user, provide a comfortable range for the user to work in. Depending upon the application, 

the system can be operated in the positive or negative pressure state. 

Definition of Terms 

Box Pressure Current pressure within the glovebox. 

Working Range The range within the working setpoints at which the box 

pressure fluctuates. 

Upper Working Setpoint 

Lower Working Setpoint 

Adjustable setpoint parameters used to establish the working 

range. 

Limit Setpoint 

The fixed value range between which the working parameters 

can be set. This is a factory established range which cannot be 

adjusted. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-2

3.3 Purging the System 

Purging the glovebox replaces the ambient air inside the box with the working gas by providing 

a constant flow of working gas into and out of the box. The purge function can be performed 

either manually by the user or automatically by the system. The automatic or “quick purge” 

function is not included with all systems but is an available option that can be purchased at any 

time after the glovebox has been installed and/or running for any period of time. 

As a general guideline a system should be purged when the oxygen level inside the box 

exceeds 100ppm. Possible reasons for an elevated oxygen level are: 

• Initial commissioning of the system; 

• Recent service or repair made to the system; 

• Room atmosphere entering the box due to improper use; 

• Leaks in the system or other damage (e.g. torn gloves, etc.). 

The purging process can be performed three (3) different ways: 

Reverse Purge 

Performed on systems with no manual purge valve or automatic purge 

function. This can also be performed on systems with either of the 

mentioned options if the user chooses to do so. 

The Reverse Purge method purges the working gas directly into the 

user’s room environment, use caution when working in the vicinity of 

a glovebox undergoing reverse purge. 

Manual Purge 

Performed on systems with a manual purge valve located on the top of 

the glovebox system. This valve should be vented to a fume hood or 

facility exhaust system. 

Automatic Purge 

Performed on systems with this function incorporated into the PLC 

program via the VSA valve. This valve should be vented to a fume hood 

or facility exhaust system. 

Both reverse purge and manual purge procedures require the user to enter box pressure 

setpoints in the PLC prior to beginning operation. The automatic purge function does not require 

any parameters to be set prior to beginning the operation. 

Purge time and gas consumption varies and is based on glovebox volume and the level of purity 

required in the glovebox. 

Figure 1 - Purge Gas Consumption 

Figure 1 above shows that a desired box atmosphere purity of 10ppm requires approximately 

14.50m3 of purge gas per cubic meter of box volume (refer to the Box Volume Table below): 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-3

Box Volume Table

Box Length 1250 Box Length 1500 Box Length 1800 

Box Depth 780 28.86 f3

 / .81 m3 34.63 f3/ .98 m3 41.56 f3 / 1.18 m3

Box Depth 1000 35.51 f3

 / 1.00 m3 42.61 f3 / 1.21 m3 51.13 f3 / 1.45 m3

Box Depth 1200 43.45 f3

 / 1.23 m3 52.14 f3 / 1.48 m3 62.57 f3 / 1.77 m3

3.4 Circulation Mode 

Circulation mode is the normal operation mode for working within the glovebox system. During 

normal operation working gas is continuously circulated between the glovebox and the gas 

purification system. As the working gas passes through the filter column the oxygen and 

moisture content is scrubbed from the gas. This provides the user with a consistent inert 

glovebox environment in which to work. 

Figure 2 - Principle of Circulation

3.5 Regeneration Mode 

When the glovebox is used for an extended period of time, oxygen and moisture begins to 

accumulate inside the purifier filter column which decreases the effectiveness of the circulation 

process. Regeneration is the process of removing the excess oxygen and moisture from the 

filter bed material inside the purifier column. This is a multi-step 16 hour process during which 

the glovebox cannot be used for normal operation (circulation). 

The regeneration procedure should not be interrupted at any time 

during the process. If for any reason the process is terminated 

prematurely, do not restart any system processes. Contact the 

M.Braun Service Department immediately. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-4

3.6 Antechamber Operation 

General Information 

The system is equipped with a large (and/or small) antechamber designed for transferring 

material into or out of the glovebox without polluting the internal box atmosphere or the room 

atmosphere. The antechamber(s) is equipped with an inner door, outer door and sliding tray to 

ease in transferring materials. 

Transferring material into and out of the box can be done in the Automatic or Manual Modes. 

Both modes utilize the antechamber components and the touch screen. The chamber 

undergoes several evacuation/refill cycles to clear the chamber of room or glovebox 

atmosphere prior to removing the material from the chamber. 

• An evacuated chamber cannot be opened. Attempting to open an 

evacuated antechamber may damage the door locking 

mechanism. 

• Keep both antechamber doors closed when not transferring 

materials. 

Method of Operation 

Open outer door and transfer material into antechamber Close outer door 

Evacuate antechamber 

Refill antechamber with box gas 

Repeat the Evacuate and Refill antechamber 

steps according to the guidelines listed in 

Chapter 9 Section 9.4 Antechamber Operation. 

Open inner door and transfer material into glovebox 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-5

Important Notes 

• Never open the inner and outer antechamber doors 

simultaneously. 

• Never open the inner door of an antechamber filled with room 

atmosphere. This would result in pollution of the box atmosphere 

which could cause damage to instruments and/or materials 

inside the box. 

• Depending on the materials used inside the glovebox, vapors 

may be released into the room when material is transferred into 

or out of the glovebox. 

Material transferred between the glovebox and room atmosphere 

must be able to withstand the pressure difference during the 

antechamber purge process (e.g. partially filled vessels, etc.). Open 

the seal slightly (e.g. lids of bottles, etc.) to allow the vessel to be 

vented to prevent implosion. 

3.7 Analyzers 

General Information 

This section applies only to systems that are equipped with oxygen and/or moisture analyzers. 

Each analyzer contains a sensor head that is protected with a metal cap and electronics that are 

used to measure (depending on the analyzer type) the oxygen or moisture content of the 

working gas inside the glovebox. The analyzers are connected to the PLC via a cable with an 

RJ45 connector. 

While not all systems are equipped with one or both analyzers at the time of initial installation 

they can be purchased at any time. Please contact the M.Braun Sales or Service Department 

for further information.

Oxygen Analyzer (MB OX-SE-1©) 

The oxygen analyzer, with a measuring range of 0 – 

1,000ppm, monitors the atmosphere inside the glovebox for 

residual oxygen content. The analyzer is designed to provide 

exact readings within the range of 0 – 100ppm. An estimated 

reading of the oxygen content is provided when the oxygen 

level rises above 100ppm. 

The sensor element is a miniaturized Zirconium Dioxide plate 

operated at elevated temperature that is controlled by a 

platinum resistor. The electronics are supplied with 24 V DC 

and deliver a 0 – 10V signal proportional to the concentration 

of Oxygen. An additional input for the electronics allows the 

switching on and off of the sensor heating by means of the 

PLC.1

Contact with Hydrogen, solvents and other aggressive gaseous 

substances will cause permanent damage to the sensor. 

 

1

 M.Braun, Inc., Sales Literature, MB OX-SE1© Oxygen Probe, (Edition 2/2004) 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 3: Principles of Operation 

© MBraun, Inc. Edition 1/2007 Page 3-6

Moisture Analyzer (MB MO-SE-1©) 

The moisture analyzer, with a measuring range of 0 – 

350ppm, monitors the atmosphere inside the glovebox for 

residual moisture content. The analyzer is designed to 

provide exact readings within the range of 0 – 50ppm. An 

estimated reading of the oxygen content is provided when the 

oxygen level rises above 50ppm. 

The sensor element consists of two (2) parallel platinum coils 

coated with phosphoric acid, and a PT1000 temperature 

sensor. The electronics are supplied with 24VDC and deliver 

a 0 – 10V signal in proportion to the concentration of H20.2

 

2 M.Braun, Inc., Sales Literature, MB MO-SE1©

 Oxygen Probe, (Edition 2/2004) 
Chapter 4

Touch Panel Operation

4.1 General Information....4-1

4.2 Input and Password Fields....4-1

4.3 Displaying the System Information....4-2
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 4: Touch Panel Operation 

© MBraun, Inc. Edition 1/2007 Page 4-1

CHAPTER 4: TOUCH PANEL OPERATION

4.1 General Information 

The glovebox system utilizes a Touch Screen Display panel. All functions and/or selections are 

performed by lightly touching the button or icon on the screen. 

An optional protective self adhesive foil is available for the touch 

screen. This protects the screen from scratches, dirt and grime.

This protective foil also provides a matte surface to reduce glare 

and can be removed at any time without leaving any residue on the 

screen.

Do not use any sharp or pointed objects to remove the protective 

foil as this may result in damage to the touch screen. 

4.2 Input and Password Fields 

Input Fields 

Certain screens contain information which can 

be changed by the user. This information is 

entered using an alphanumeric pad which is 

displayed when an input field is touched. 

When an input field is activated, a keyboard is 

displayed on the screen. 

Using this keyboard, you can enter new values 

into an input field.

After completing the entry, press the enter key to 

confirm the value. 

Password Fields 

Certain input fields require a user password to be 

entered. When an input field requiring a password is 

touched, a password screen is displayed. To enter the 

password, touch the white area in the screen. Only 

authorized users can make changes to the input fields. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 4: Touch Panel Operation 

© MBraun, Inc. Edition 1/2007 Page 4-2

4.3 Displaying the System Information 

Touch the Common Parameters button on 

the Start Screen. 

Touch the System button to access the 

System settings screen. 

Touch the Info button. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 4: Touch Panel Operation 

© MBraun, Inc. Edition 1/2007 Page 4-3

The Information Screen is displayed 

providing M.Braun contact information and 

information regarding the project number or 

serial number for the system. 
Chapter 5

System Screens

5.1 General Information....5-1

5.2 Start Screen....5-1

5.3 Common Parameters Screen....5-3

5.4 Alarm Setpoints Screen....5-4

5.5 Min./Max. Values Screen....5-4

5.6 Functions Screens....5-5

5.7 Antechamber Screen....5-7

5.8 Antechamber Parameters Screen....5-8

5.9 Antechamber Trends Screen....5-9

5.10 Box Pressure Parameters Screen....5-9

5.11 Purifier Layout Screen....5-10

5.12 Purifier Parameter Screen....5-11

5.13 Purifier Status Screen....5-11

5.14 Operating Hours Screen....5-12

5.15 Purifier Trends Screen....5-12

5.16 System Screen....5-13

5.17 Box Purging Screen....5-13

5.18 Freezer/Box Cooling Parameters Screen....5-14

5.19 Warning Screen....5-11
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-1

CHAPTER 5: SYSTEM SCREENS

5.1 General Information 

The system is operated using a Touch Screen located on the side of the system above the 

antechamber. This chapter provides an introduction to the various screens that the user will 

access during operation of the system. 

To activate a component or operation touch the corresponding button. To deactivate a 

component or operation touch the corresponding button again. 

Red buttons and icons represent inactive functions, green buttons and icons represent active 

functions and gray buttons represent functions which are nonexistent or temporarily unavailable. 

The purifier icon will also be displayed in yellow signaling the regeneration process is active. 

The list below displays the buttons and information fields which are located on multiple screens 

associated with the system. 

Button / Information Field Description 

Displays the Alarm Screen. Blinks black & red when an alarm 

is present. 

Displays the actual glovebox pressure. 

Displays the moisture level inside the glovebox as detected by 

the analyzer. 

Displays the oxygen level inside the glovebox as detected by 

the analyzer. 

Displays the previous screen. 

Displays the Start screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-2

5.2 Start Screen 

Main screen displayed on the touch 

panel. 

All functions and screens related to the 

operation of the glovebox and other 

components are accessed via the Start 

Screen. 

Icon / Button Description 

Displays the status of the blower. 

Displays the status of the vacuum pump. 

Purifier 

Inactive 

Circulation 

Active 

Regeneration 

Active 

Displays the status of the purifier. 

Used to access the Purifier Layout screen. 

Displays the current pressure inside the glovebox. 

Displays the Moisture and/or Oxygen levels inside the 

glovebox as determined by the analyzer(s) when present. 

Touching any area in this box will display the Box Pressure 

Parameters Screen. 

Displays the actual freezer temperature. This field is not 

displayed when there is no freezer present on the system. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-3

5.3 Common Parameters Screen 

Accessed via the Start Screen by 

touching the Common Parameters

button. 

Button Description 

Displays the Alarm Setpoints screen. 

Displays the analyzer Min./Max. Values screen. 

Displays the Operating Hours screen. 

Displays the Box Trends screen. 

Displays the System information screen. 

Displays the Service screen. 

Displays the Box Purging Screen. 

Displays the Freezer/Box Cooling parameters screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-4

5.4 Alarm Setpoints Screen 

Accessed via the Common Parameters 

screen by touching the Alarm Limits

button. 

Button Description

Used to set the alarm parameter for the 

moisture analyzer when present on the 

system. 

Used to set the alarm parameter for the 

oxygen analyzer when present on the 

system. 

Used to set the alarm parameter for the 

freezer when present on the system. 

Used to set the alarm parameter for the box 

cooling unit when present on the system. 

5.5 Min./Max. Values Screen 

Accessed via the Common Parameters 

screen by touching the Min./Max. Values

button. 

This screen displays the highest and 

lowest oxygen and moisture levels as 

detected by the analyzers. 

Use the Reset button to reset the 

displayed values to zero. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-5

5.6 Functions Screens 

Not all functions shown on this screen are included with every 

system. In the event of a component or function not being included, 

the button will not be displayed on this screen. 

Functions Screen 1 

Accessed via the Start Screen by 

touching the Functions button. 

Circulation Purifier and Regeneration 

Purifier 2 buttons are permanently grayed 

out for single purifier systems. 

Button Description

Used to activate and deactivate the 

circulation process. 

Used to activate the regeneration 

process. 

Used to activate and deactivate the 

vacuum pump. 

Used to activate and deactivate the 

analyzer(s). (This button is grayed out 

when analyzers are not present on 

the system.) 

Used to activate the automatic purge 

process. 

Used to turn the light on and off. 

Used to turn the freezer on and off. 

(This button is only present when 

there is a freezer on the system.) 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-6

Functions Screen 2 

Accessed via Functions Screen 1 by 

touching the Next button. 

Antechamber 2 buttons are permanently 

grayed out when only one large 

antechamber is present on the system. 

Button Description

Used to start an automatic antechamber cycle for 

the large antechamber. 

Used to evacuate the large antechamber in 

manual mode. 

Used to refill the large antechamber in manual 

mode. 

Used to activate the vacuum pump for the 

antechamber. (This is activated automatically 

when the antechamber is operated using the 

purifier vacuum pump.) 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-7

5.7 Antechamber Screen 

Accessed via the Start Screen by 

touching the Antechamber icon. 

This screen is used to operate the large 

antechamber only. 

Information Field / Icon / Button Description 

Displays the active step number and step description 

when the system is being run. 

Identifies which mode the system is being run in. 

Displays the actual pressure inside the chamber. 

Displays the status of the pressure level inside the 

antechamber. 

Displays the status of the refill valve. 

Displays the status of the evacuation valve. 

Displays the status of the vacuum pump. 

Used to activate and deactivate the various components 

and processes. These buttons work the same as the 

buttons located on the Functions screen. 

Used to access the antechamber parameters screen. 

Used to access the antechamber trends screen. 

Used to operate the antechamber in Automatic Mode. 

Used to operate the antechamber in Manual Mode. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-8

5.8 Antechamber Parameters Screen 

Accessed via the Antechamber Screen 

by touching the Parameters button. 

Information Field / Icon / Button Description 

Level at which the antechamber refills during 

the automatic evacuation/refill cycle. 

The level at which a leak test is performed on 

the antechamber in automatic mode. 

Level at which the refill process of the 

antechamber is initiated in automatic mode. 

Number of intermediate refill cycles performed 

in automatic mode. 

The maximum amount of time allowed for the 

antechamber to evacuate to the vacuum set 

point. 

Level at which the system alarm will display, 

above the vacuum leak test set point value. 

For example if the setpoint is 5x10-1 mbar and 

the maximum leak rate value is 3, the alarm 

will not display until the set point reaches 

8x10-1 mbar. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-9

5.9 Antechamber Trends Screen 

Accessed via the Antechamber Screen by 

touching the Trends button. 

Use the various buttons on the screen to scroll 

through the designated time period and also to 

zoom in on a selected range over a 24 hour 

period. 

5.10 Box Pressure Parameters Screen 

Accessed via the Start Screen by 

touching the Box Pressure and 

Analyzer Details field. 

Information Field Description 

Used to enter the upper working box pressure setpoint. 

Used to enter the lower working box pressure setpoint. 

Displays the setpoint at which the box pressure will rise 

or drop when the actual box pressure reaches the upper 

or lower working limit. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-10

Information Field Description 

Displays the default pressure alarm limits for the system. 

Displays the Freezer/Box Cooling screen. 

5.11 Purifier Layout Screen 

Accessed via the Start Screen by 

touching the RKM icon. 

Valve Definitions 

VHE1 

VHA1 

VRE1 

VRS 

VS1/2 

VG 

VRV 

VV 

VPG 

- 

- 

- 

- 

- 

- 

- 

- 

- 

Purifier Inlet 

Purifier Outlet 

Regen. Gas Inlet 

Regen. Gas Outlet 

Pressure Compensation 

Working Gas Inlet 

Regen. Vacuum 

Box Vacuum 

Vacuum Pump 

Icon / Information Field / Button Description 

Displays the status of the various valves throughout the 

system. 

Displays the status of the blower. 

Displays the status of the vacuum pump. 

Purifier 

Inactive 

Circulation 

Active 

Regeneration 

Active 

Displays the status of the purifier. 

Displays the active step of the regeneration process. 

Used to activate and deactivate the various components 

and processes. These buttons work the same as the 

buttons located on the Functions screen. 

Used to access the purifier parameters screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-11

5.12 Purifier Parameter Screen 

Accessed via the Purifier Layout screen 

by touching the Parameters button. 

Choose Yes to have circulation start 

automatically after the regeneration 

process is completed. 

Choose No to start circulation manually 

after the regeneration process is 

completed. 

5.13 Purifier Status Screen 

Accessed via the Purifier Parameter 

screen by touching the Status button. 

Button / Information Field Description 

Displays the current mode being used to operate 

the system. 

Displays the time remaining until the 

regeneration process is complete. 

Displays the number of hours the system has 

been operating in circulation mode since the last 

regeneration process was performed. 

Displays the total hours the purifier has been in 

operation. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-12

5.14 Operating Hours Screen 

Accessed via the Common Parameters 

screen by touching the Operating 

Hours button. 

Displays the number of hours which the 

components have been running. 

Under normal use the vacuum pump oil should be changed every 

3,000 hours per the manufacturer’s recommendation. 

5.15 Purifier Trends Screens 

Accessed via the Common Parameters 

screen by touching the Trends button. 

Use the various buttons on the screen to 

scroll through the designated time period 

and also to zoom in on a selected range 

over a 4 hour period. 

Touch the 24h button to change the 

display range to a 24 hour period. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-13

5.16 System Screen 

Accessed via the Common Parameters 

screen by touching the System button. 

Used to display system information or 

perform maintenance on the touch 

screen. 

5.17 Box Purging Screen 

Accessed via the Common Parameters 

screen by touching the Box Purging

button. 

Information Field Description

Used to automatically purge the glovebox 

when the oxygen level reaches the Oxygen 

Limit Value setpoint entered below. 

Value at which the purging process will initiate 

when the above field is set to Yes. 

Used to automatically initiate the purge 

procedure when the box pressure reaches the 

factory established Lower Limit Setpoint. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 5: System Screens 

© MBraun, Inc. Edition 1/2007 Page 5-14

5.18 Freezer/Box Cooling Parameter Screen 

Accessed via the Common Parameters 

screen by touching the Freezer Box 

Cooling button. This screen is also 

accessed via the Box Pressure 

Parameters by touching the More

button. 

This screen applies only to systems with 

a freezer and/or box cooling unit. 

Information Field Description 

Used to set the freezer operating temperature. 

Displays the actual temperature inside the 

freezer. 

Used to set the box cooling unit operating 

temperature. 

Displays the actual temperature inside the 

glovebox. 

5.19 Warning Screen 

Accessed via any screen by touching the 

Alarm button when displayed. 

This screen lists the: 

• Warning number; 

• Time the error occurred; 

• Description of the error. 

Depending upon the type of alarm, the 

message: 

• Will disappear upon fixing the error; 

• Must be acknowledged by touching 

the ACK button after the error has 

been fixed before it will disappear. 
Chapter 6

Installation

Requirements

6.1 Location....6-1

6.2 Gas Supply and Connection Requirements....6-1

6.3 Venting Requirements....6-2

6.4 Electrical Connection Requirements....6-2

6.5 Water Cooling Requirements....6-2
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 6: Installation Requirements 

© MBraun, Inc. Edition 1/2007 Page 6-1

CHAPTER 6: INSTALLATION REQUIREMENTS

6.1 Location 

The room in which the glovebox will be used should be dry and well ventilated with a 

temperature between 59°F and 86°F (15°C to 30°C). The floor should be level and stable 

enough to support the weight of the glovebox system. 

Position the glovebox in an area that allows the user to have adequate working space in and 

around the system (e.g. gloveports, antechamber(s), etc.). If positioned against or near a wall, 

the clearance should be a minimum of approximately 24 inches (600 mm) for the rear and side 

of the glovebox without antechamber(s). 

6.2 Gas Supply and Connection Requirements 

Working Gas 

Use: To establish and maintain the ultra pure gas atmosphere. Used as the 

operating gas for electropneumatic valves. 

Gas Type: Nitrogen, Argon, Helium supplied at 80psi. 

Purity: Industrial grade or better; from tanks or other facility gas supply source. 

Quantity: Permanent supply for the system’s operation (e.g. pressure compensation, 

etc.). 

Regulator Type: Two (2) stage with a 0 – 100psi gauge. 

Pressure: 80psi 

Flow rate: 200 liters/minute 

Material: 3/8” Braided TygonTM hose (supplied with the system). 

Connections: 1⁄4” male NPT to 3/8” O.D. hose barb (connects TygonTM hose to gas 

regulator). 

Regeneration Gas 

Use: Reprocessing saturated purifier columns. 

Gas Type1: 

Nitrogen as the Working Gas: 90-97% Nitrogen with 3-10% Hydrogen 

balance 

Argon as the Working Gas: 90-97% Argon with 3-10% Hydrogen balance 

Helium as the Working Gas: 90-97% Helium with 3-10% Hydrogen balance

Quantity: Approximately 3,500 liters for each regeneration process. 

Regulator Type: Two (2) stage Hydrogen regulator with a 0 – 20psi gauge. 

Pressure: 5psi 

Flow Rate: 15 – 20 liters/minute 

Material: 3/8” Braided TygonTM hose (supplied with the system). 

Connection: 1⁄4” male NPT to 3/8” O.D. hose barb (connects TygonTM hose to gas 

regulator). 

 

1

 Gas combination will vary depending upon the type of working gas used based on user’s application. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 6: Installation Requirements 

© MBraun, Inc. Edition 1/2007 Page 6-2

6.3 Venting Requirements 

Venting to the glovebox system and vacuum pump to the facility ventilation exhaust system is 

not mandatory but is recommended. 

Vacuum Pump: 1” inner diameter braided TygonTM (or similar type) hose. 

Regeneration Exhaust Gas: 3/8” inner diameter braided TygonTM (or similar type) hose. 

Purge Valve† ‡: 13⁄4” inner diameter braided TygonTM (or similar type) hose. 

†

 This is an optional item which is not included with all systems. 

‡

This applies to both automatic and manual purge valve types. 

6.4 Electrical Connection Requirements 

The power supply for the system will vary according to local area guidelines. Please refer to the 

system label located on the side of the box in the upper right hand corner above the 

antechamber for information specific to the system. 

Type NEMA 5-20R non GFCI outlet(s) are required for all electrical 

connections. 

6.5 Water Cooling Requirements 

The glovebox system includes a heat exchanger which is required to be connected if the box 

atmosphere is to be cooled. 

Temperature: 15°C - 25°C 

Flow Rate: 2 liters/minute at 10°C, 5 liters/minute at 15°C 

Inlet Pressure: 2.0 – 4.0 bar 

Outlet Pressure: Depressurized (maximum 0.5mbar) 

Material: 3/8” Braided TygonTM (or similar type) hose. 
Chapter 7

Installation

7.1 General Information....7-1

7.2 Tools and Materials Required....7-1

7.3 Assembling the Glovebox....7-3

7.4 Connecting the Antechamber Gauges and Sensors....7-3

7.5 Electrical Feedthorugh and Power Strip....7-4

7.6 Stand Mounted Purifier.....7-6

7.7 Stand Alone Purifier....7-8

7.8 Attaching the Vacuum Pump Oil Mist Filter....7-11

7.9 Attaching the Gloves....7-12

7.10 Connecting the Freezer and Compressor....7-13

7.11 Attaching the Analyzers....7-14

7.12 Lowering the Leveling Feet....7-15

7.13 Power and Gas Supply....7-16
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-1

CHAPTER 7: INSTALLATION

7.1 General Information 

The M.Braun glovebox has been fully assembled, operated and tested at our production facility. 

The information binder contains the: 

• Glovebox Project Checklist – Displays detailed information regarding the glovebox 

system 

• Test Sheet – displays the various operations and components which were tested during 

production 

• Product Identification and Traceability – Lists all serial numbers for the components 

included on the system 

• Vendor Component Manuals – Operating instructions, etc. for components included on 

the system which are not manufactured by M.Braun. 

The glovebox and antechamber(s) have been fully tested for leaks under positive and negative 

pressure. The vacuum level during the regeneration process has also been tested to ensure 

that the glovebox purifier performs optimally. 

This chapter provides information regarding the actual assembly of the glovebox prior to use. All 

instructions included in this chapter should be strictly adhered to. Any questions that arise 

during the installation process should be directed to the M.Braun Service Department. 

The Unilab system comes complete and ready to assemble. The components, which have been 

numbered for ease in assembly, should be installed in the order listed in these instructions not 

in the order which they are numbered. 

All clamp and o-ring assemblies used to 

attach pipes and hoses should be tightened 

finger tight. 

Prior to tightening the clamp onto the flange, 

ensure the o-ring and other components are 

seated properly to avoid leaks. 

Figure 1 - Clamp, Flange, O-Ring Assembly 

7.2 Tools and Materials Required 

Tools (not supplied by M.Braun): 

• 13mm Wrench • 18mm wrench 

• 19mm Wrench • Adjustable Wrench

• Flathead Screwdriver
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-2

Materials: 

The quantity for each material or component listed below will vary depending upon the length 

of the system (i.e. 1250/780, 2500/1000, etc.). Please refer to the checklist included with the 

system for exact quantities.

Gloves KF25 Clamp KF40 Clamp 

Glove O-ring KF25 O-ring KF40 O-ring 

Electrical Feedthrough Power Strip 3/8” Braided Tygon® Tubing 

KF25 Stainless Steel 

Flexible Hose 

1” Pipe 

(copper or stainless steel) Hose Clamp 

Vacuum Pump Oil Mist Filter 

Quantity (1) per vacuum pump 

Funnel O-ring 

Quantity (1) per vacuum pump 

Oil 

Quantity (1) bottle per vacuum pump 

U-shaped clamp, O-ring, Bolt and Thumb Screw for 

Oil Mist Filter 

Quantity (1) each per vacuum pump 

O2 Analyzer 

Optional Item 

H2O Analyzer 

Optional Item 

Manual Purge Valve 

Optional Item 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-3

7.3 Assembling the Glovebox 

General Information 

A packing checklist has been included with the system. Most components included on this 

checklist have been packed in the antechamber. Prior to beginning the installation process, 

unpack, unwrap and identify each component and ensure the appropriate number of items has 

been included with the shipment as indicated on this checklist. Contact the M.Braun Service 

Department if any of the items are missing or damaged. 

Place all components in an easily accessible location close to the glovebox in an organized 

manner to ease in the assembly process. 

This chapter contains assembly information for the Labmaster© 130 (stand mounted purifier) 

and MB20G (stand-alone) style gloveboxes. Please refer to the pictures below to determine the 

type of system and follow the corresponding assembly instructions. 

Figure 2 - Stand Mounted Purifier Figure 3 - Stand-Alone Purifier 

Section 7.6 provides instructions for installing a Stand Mounted purifier glovebox system. 

Section 7.7 provides instructions for installing Stand-Alone purifier glovebox systems. Sections 

7.4-7.5 and 7.8 through 7.13 provide instructions which pertain to both types of glovebox 

systems. 

Prior to beginning the installation process move the system into the permanent location but 

allow enough clearance during the installation process to work behind the box and/or purifier. 

These installation instructions include all possible components for a 

glovebox system. Please note, not all components are included 

with every system. 

7.4 Connecting the Antechamber Gauges and Sensors 

The gauges and sensors depicted below will depend on the options purchased with the system. 

Please refer to your Project Checklist for more information. 

The gauges are attached with KF16 clamps and o-rings and the electrical connections are made 

by attaching the corresponding labeled plug from the system to the gauge and/or sensor. The 

picture below provides information for the various gauges and the completed assembly. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-4

Figure 4 – Antechamber Valve, Gauge & Sensor Assembly

A. Evacuation Valve (VSV) – Standard on an LM130 and optional on an MB20G 

B. Refill Valve (VSF) – Standard on an LM130 and optional on an MB20G 

C. Pirani Gauge (GP114) – Present when auto antechamber control option is included. 

D. Rough Gauge (GP113) – Present when auto antechamber control option is included. 

7.5 Electrical Feedthrough and Power Strip 

There are multiple flanges located on the back of the glovebox which are used to attach 

feedthroughs to allow components to be used inside the glovebox. Any one of these can be 

used for the supplied electrical feedthrough. 

Select one feedthrough that is close to the 

main power supply (outlet) and remove the 

clamp, blank cap and o-ring. 

Place the o-ring on the female connector side 

of the feedthrough. 

A

B

C

D
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-5

Place the female connector end of the 

feedthrough into the flange and feed into the 

box until the feedthrough flange is flush with 

the box flange. 

Place the clamp around the flange and tighten. 

Place the 6 outlet power strip inside the 

glovebox through one of the gloveports located 

on the front of the box. 

Plug the power strip into the feedthrough. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-6

7.6 Stand Mounted Purifier 

Systems with stand mounted purifiers only require the vacuum pump and other miscellaneous 

components to be connected prior to use. Please refer to sections 7.7 through 7.<> for 

instructions on connecting the other components pertaining to the system. 

Connecting the Vacuum Pump 

Attach the KF25 stainless steel flexible hose 

to the end of the 1” pipe with the port using a 

KF25 clamp and o-ring. 

Attach the pipe to the valve located on the 

bottom of the rear side of the antechamber 

using a KF40 clamp and o-ring. 

Attach the stainless steel flexible hose to the 

port on the vacuum pump with the funnel 

shaped o-ring using a KF25 clamp. 

Attach the fitting on the purifier vacuum hose 

(H) to the port on the copper or stainless pipe 

and tighten hand tight. 

Using the 19mm and 18mm wrenches 

Tighten an additional 1⁄4 turn. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-7

If system is equipped with a mini 

antechamber: 

Attach the end of the vacuum hose (H) with 

the elbow to the port located on the rear side 

of the mini antechamber. 

Use care not to cross thread brass to brass connections and 

stainless steel to stainless steel connections when fitting together 

and tightening. 

7.7 Stand Alone Purifier 

Below is a rear view of the purifier showing the various components and cables which require 

connection to the glovebox. Please refer to these pictures when following the instructions. 

Figure 5 - Purifier Layout Figure 6 - Completed Purifier Setup 

Purifier Cart 

A. PLC-Touch Screen Panel and Housing 

B. Inlet Hose from Glovebox to Purifier 

C. Outlet Hose from Blower to Glovebox 

D. Lighthood Cable 

E. Pressure Sensor Cable 

F. Ground Wire 

G. Freezer Connection Cable 

H. Purifier Vacuum Hose 

I. Main Power Cable 

J. Foot Pedal 

A 

B 

C

D

E

F 

G

H 

I J
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-8

Connecting the Purifier 

After the purifier is attached, it is possible to move the glovebox 

forward and backward (to allow additional connections to be made) 

but side to side will be extremely difficult. Therefore, it is 

recommended that prior to connecting the purifier, the glovebox be 

moved into the final location where it is to be used. 

Roll the purifier cart into place under the antechamber. 

The recommended distance between the front leg of 

the stand and the electrical cabinet is 2 1⁄2 “–3”. 

Remove the bolt from the black PLC bracket located 

on the end panel where the antechamber(s) is located. 

Place the hinge side of the touch panel enclosure 

between the openings of the bracket. 

Affix the enclosure to the bracket using the bolt, by 

guiding it down and through the holes in the bracket 

and hinge. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-9

Secure the hinge by using the 13mm and adjustable 

wrenches. 

Attach the KF40 stainless steel flexible hose (B) from 

the VHE valve on the filter column to the circulation 

pipe using a KF40 clamp and o-ring. 

Attach the “Out” KF40 stainless steel flexible hose (C) 

on the blower to the bottom of the heat exchanger 

using a KF40 clamp and o-ring. 

Connecting the Ground Wire 

Using the 13mm wrench remove the 

grounding bolt located on the inside of the 

rear leg of the stand adjacent to the purifier 

cart by turning in a counterclockwise motion. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-10

Remove the two bottom washers from the 

bolt. 

Place the end of the ground wire (F) onto the 

bolt. 

Place the washers onto the bolt with the 

toothed washer teeth pointing towards the 

leg of the stand. 

Screw the bolt back into the leg by turning in 

a clockwise motion. 

7.8 Attaching the Vacuum Pump Oil Mist Filter 

Place the black o-ring onto the remaining 

port on the vacuum pump. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-11

Place the oil mist filter onto the port. 

Push the u-shaped clamp onto the port 

making sure it covers both the port and 

filter flange. 

Turn the clamp so the open ends face 

out. 

Secure in place with the bolt and red 

thumb screw. Tighten hand tight. 

Turn the filter so the label faces the gas 

ballast on the top of the vacuum pump. 

Push the black hose from the pump onto 

the port on the filter. Secure in place with 

black plastic clamp. 

7.9 Attaching the Gloves 

Slide the glove onto hand and insert arm into 

the box through the gloveport angling the 

thumb to a comfortable working position. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-12

Slide hand out of the glove and work the cuff 

of the glove into the groove in the gloveport 

closest to the glass. At this time the thumb 

position can still be adjusted by rotating the 

cuff of the glove. 

Secure the glove with two o-rings. 

Place the o-ring in the top of the second 

groove from the glass and in one smooth 

motion pull down across the circumference 

of the gloveport. 

Repeat the process for the remaining glove(s) 

and o-ring(s). 

7.10 Connecting the Freezer and Compressor 

General Information 

This section applies only to systems that are equipped with freezers. 

Remove the cover from the horizontal wire 

track located on the rear of the stand. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-13

Run the freezer connection cable (G) along 

the inside of the wire track. 

Place the end of the cable on the stand and 

replace the wire track cover. 

Connect the power supply plug for the 

compressor. 

 

The connector type will vary from system to system. The picture 

shown above displays the two possible connector types that will be 

used. Contact the M.Braun Service Department with any questions. 

7.11 Attaching the Analyzers 

General Information 

This section applies only to systems that are equipped with analyzers. 

Carefully place a KF40 o-ring on the analyzer 

port. 

Carefully place the sensor end of the analyzer 

into the corresponding port on the circulation 

piping running the length of the system on the 

underside of the glovebox. 

H2O analyzer is positioned on the side of the 

box opposite the antechamber(s). 

O2 analyzer is positioned on the same side of 

the box as the antechamber(s). 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-14

Secure the analyzer to the pipe using a KF-40 

clamp and o-ring. 

Repeat this process with the remaining analyzer(s).

Insert the analyzer cable plug into hole on the 

corresponding analyzer by pushing the plug 

into the hole gently until it snaps into place. 

The Green plug attaches to the H2O Analyzer. 

The Blue plug attaches to the O2 Analyzer. 

 

The green plug cable should be wrapped along the circulation pipe 

and then plugged into the moisture analyzer. 

7.12 Lowering the Leveling Feet 

Move the system into its permanent location. 

Lower each of the leveling feet by hand until 

they touch the ground. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 7: Installation 

© MBraun, Inc. Edition 1/2007 Page 7-15

Using a 13mm wrench continue to lower the 

leveling feet until the casters are raised off the 

ground. 

7.13 Power and Gas Supply 

Plug the main power cable (I) on the purifier into the main power supply outlet. 

Attach the working gas line from the purifier to the working gas supply. 

Attach the regeneration gas line from the purifier to the regeneration gas supply. 

Place the foot pedal on the floor in front of the system in an easily accessible location. 

The installation is now complete. 
Chapter 8

Commissioning the System

8.1 General Information....8-1

8.2 System Activation....8-1

8.3 Static Pressure Test....8-2

8.4 Purging the System....8-4
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-1

CHAPTER 8: COMMISSIONING THE SYSTEM

8.1 General Information 

After completing the installation, the system is ready to be activated. Gas can now be added to 

the box to prepare for normal operation. The system is equipped with a foot pedal which is used 

during a pressure leak test or during normal operation of the box to temporarily increase or 

decrease the pressure inside the glovebox. 

While a leak test has been performed during production of the system, after initial installation 

and gas has been added, it is necessary to perform a static pressure test to check the system 

for any possible leaks. This is done to ensure all connections made during the installation are 

tight and seated properly. The static pressure test also checks other components on the system 

which may have shifted during the shipping process. 

After the leak test has been completed, the purge operation must be performed to rid the box of 

all room air and replace it with the chosen working gas. After the purge procedure is complete, 

the box can be used for normal operation in circulation mode. 

8.2 System Activation 

Turn the Main Power switch located on the side of 

the electrical cabinet on the purifier cart from the Off

position to the On position. 

The panel will display a series of screens (program 

starting up) before the start screen is displayed. 

Touch the Functions button on the start screen. 

Touch the Vacuum Pump button to activate the 

vacuum pump. 

Touch the Box Light button to turn the light on. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-2

The vacuum pump should remain on at all times while the system is 

in operation. 

Touch the End button to return to the start screen. 

Using the regulator, turn on the working gas supply and set the pressure to 80psi. 

8.3 Static Pressure Leak Test 

The static pressure leak test is performed in both the positive and negative pressure states. The 

process for running the test in either state is the same with the exception of the parameter 

setpoints and foot pedal controls. 

Touch the Box Pressure/Analyzer display box on 

the start screen. 

Use the arrows listed under Upper Working Limit 

and Lower Working Limit to increase or decrease 

the box atmosphere pressure setpoint in one tenth 

decimal intervals. 

or 

Touch the number field next to the corresponding 

parameter to display the alphanumeric keypad. 

Recommended Positive State Setpoints 

• Upper Working Limit: 14.0mbar 

• Lower Working Limit: 0.0mbar 

Recommended Negative State Setpoints 

• Upper Working Limit: -14.0mbar 

• Lower Working Limit: 0.0mbar
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-3

Enter the desired limit and touch the 

button to return to the Box pressure screen. 

Use the button to set the parameter to the 

positive or negative. 

Touch the End button on the Box Pressure 

Parameters screen. 

Using the foot pedal control, adjust the gas pressure inside the box until the pressure reaches 

the desired level range. 

The pressure will fluctuate until it settles at a fixed level between +/- 10-12mbar.

If the pressure drops below +/-10mbar, use the foot pedal to add 

gas until the pressure reaches the desired level. 

Once the pressure settles, monitor the pressure level for five (5) minutes.

It is normal for the pressure level to fluctuate slightly during the 5 

minute static pressure test. However, a pressure decrease of more 

than .3mbar indicates there is a leak in the system. Check all 

connections made during the installation process. If this doesn’t fix 

the problem, refer to the Troubleshooting chapter of this manual or 

contact the M.Braun Service Department. 

After five (5) minutes, if the pressure level has stayed constant, the glovebox can be tested in 

the opposite pressure state.

After testing the box in both the positive and negative states with no leaks present, the box is 

ready for purging.
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-4

8.4 Purging the System 

Systems equipped with freezers or other components that may be 

protected by covers must be open during the purging process. 

If the system has been in use prior to purging, ensure that the 

freezer(s) is deactivated and is at room temperature before 

beginning the process. 

The instructions below include information regarding glovebox systems equipped with 

analyzers. The analyzers, which are used to monitor the oxygen and moisture levels inside the 

glovebox, can be used to determine when the glovebox atmosphere reaches the desired purity 

level. 

Glovebox systems which are not equipped with analyzers require the gas consumption to be 

monitored to determine when the glovebox atmosphere reaches the desired purity level. Refer 

to the Gas Consumption chart in Chapter 3 for more information. 

Box Purge Volume Table

Box Length 1250 Box Length 1500 Box Length 1800 

Box Depth 780 13,000 liters 14,000 liters 18,000 liters 

Box Depth 1000 14,000 liters 18,000 liters 22,000 liters 

Box Depth 1200 18,000 liters 22,000 liters 26,000 liters 

Adjusting the Parameters 

Adjusting the parameters is only necessary when using the 

Reverse or Manual purge methods. 

Touch the Box Pressure/Analyzer display box on 

the start screen. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-5

Use the arrows listed under Upper Working Limit 

and Lower Working Limit to increase or decrease 

the box atmosphere pressure setpoint in one tenth 

decimal intervals. 

or 

Touch the number field next to the corresponding 

parameter to display the alphanumeric keypad. Use 

the keypad to adjust the parameters in the same 

manner as before. 

Recommended Setpoints 

• Upper Working Limit: 12.0mbar 

• Lower Working Limit: 9.0mbar 

Touch the End button. 

Reverse Purge Method (no manual purge valve or quick purge function present) 

Open the inner antechamber door. 

Very slowly turn the outer antechamber door handle 

in a counterclockwise motion until the glovebox 

pressure drops to a level between 1.0mbar and 

8.0mbar and the VG valve stays open* providing a 

constant flow of gas into and out of the box. 

*When the valve stops clicking it is open. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-6

Manual Purge Method (manual purge valve present) 

Open the manual hand valve located on the top of 

the glovebox. 

The VG valve will open and close to allow working gas to flow into the box. Once the pressure 

inside the box drops to a level between 1.0mbar and 8.0mbar, the valve will stay open* 

providing a constant flow of gas into and out of the box. 

*When the valve stops clicking it is open.

Automatic Purge Method (quick purge function present) 

Touch the Functions button on the start screen. 

Touch the Quick Purge button on the functions 

screen to activate the purging process. 

The circulation button is blanked out as this function 

is unavailable during the purging process. 

Continue purging for 45 minutes. 

Touch the Analyzer button to activate the 

analyzers. 

Touch the End button to return to the Start Screen.

Continue purging until the oxygen and moisture content inside the box reach the desired ppm 

levels.
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 8: Commissioning the System 

© MBraun, Inc. Edition 1/2007 Page 8-7

Touch the Quick Purge button on the functions 

screen to deactivate purging once the desired levels 

have been attained. 
Chapter 9

System Operation

9.1 General Information....9-1

9.2 Circulation Mode....9-1

9.3 Analyzers....9-3

9.4 Large Antechamber Operation....9-4

9.5 Mini Antechamber Operation....9-12

9.6 Freezers....9-15

9.7 Regeneration....9-17
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-1

CHAPTER 9: SYSTEM OPERATION

9.1 General Information 

After the system has been fully commissioned, it may now be used for normal operation in 

circulation mode. Prior to using the glovebox in circulation mode the box pressure parameters 

can be adjusted for the upper and lower working limits. There are no recommended set 

parameters, the setpoints are based on user preference. 

At any time during use of the box, the foot pedal can be used to add or remove working gas 

from the box temporarily. This is especially useful when attempting to place hands inside the 

gloves and into the box when working in the positive pressure state. 

Adjusting the parameters is done in the same manner as was done during the static leak test 

and purging during the commissioning of the box. Please refer to Chapter 8 of this manual for 

instructions. 

9.2 Circulation Mode 

• The circulation process can only be started if all other functions 

(purge and regeneration) are inactive. 

• There are no recommended setpoints for the circulation mode. 

Upper and lower working pressure limits are based on user 

preference. 

• If the oxygen level in the system rises above 150ppm, deactivate 

circulation and begin purging. Continue purging until the oxygen 

level drops below 100ppm. 

Touch the Box Pressure/Analyzer display box on 

the start screen. 

Use the arrows listed under Upper Working Limit 

and Lower Working Limit to increase or decrease 

the box atmosphere pressure setpoint in one tenth 

decimal intervals. 

or 

Touch the number field next to the corresponding 

parameter to display the alphanumeric keypad. 

Use the keypad to adjust the parameters in the 

same manner as before. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-2

Touch the End button. 

Touch the Functions button on the start screen. 

Touch the Circulation Purifier 1 button on the 

functions screen. 

The regeneration and quick purge (if existing) 

buttons are blanked out as these functions are 

unavailable during the purging process. 

Touch the End button to return to the Start Screen. 

The glovebox will continue to circulate the working gas until the function is deactivated. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-3

9.3 Analyzers 

General Information 

This section applies only to systems that are equipped with moisture and/or oxygen analyzers. 

Touch the Functions button on the Start screen.

Touch the Analyzer button to activate the 

analyzer(s). 

Touch the End button to return to the Start Screen. 

Follow the steps above to deactivate the analyzer(s).
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-4

9.4 Large Antechamber Operation 

Follow the steps below to perform a transfer of materials from room environment into the 

glovebox using the large antechamber. If equipped, the large antechamber evacuation and refill 

cycles can be operated in Automatic or Manual modes. After placing the material into the large 

antechamber please refer to the corresponding directions below for moving the material into the 

glovebox. 

Placing Material in the Antechamber 

Before opening the outside antechamber door, 

verify: 

• The inner antechamber door is closed; 

• The antechamber is not under vacuum 

(gauge (A below) should read zero). 

Turn the handle on the outer antechamber door in 

a counterclockwise direction to open it. 

Carefully open the antechamber door by lifting it in 

an upward direction. 

A

A
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-5

Let go of the door handle and allow the door to 

rise completely. 

Gently pull out the sliding tray. 

Place the materials to be used on the tray and 

slide the tray back into the antechamber. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-6

Gently push the sliding tray back into the 

antechamber. 

Close the outer door by pulling it down until 

groove at the end of the arm rests on the spindle. 

Place four fingers, two each on opposite spindles, 

on the door handle and gently turn in a clockwise 

motion until the door touches the chamber. 

Tighten 1⁄4 turn more. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-7

Automatic Mode 

Touch the Antechamber icon on the Start 

Screen to display the antechamber screen. 

Touch the Parameters button to display the 

Antechamber parameters screen. 

Use the drop down menus and information fields 

to specify the settings for the antechamber cycle. 

Touch the Back button to return to the 

Antechamber layout screen. 

Touch the Evacuate/Start Autom. button to 

initiate the automatic antechamber cycle. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-8

A message on the upper right hand corner of the 

screen displays the active step number and 

description of the cycle. 

Touch Manual button any time during the automatic antechamber 

cycle to abort the cycle. 

Manual Mode 

Touch the Antechamber icon on the Start 

Screen to display the antechamber screen. 

If Automatic Mode is displayed in the upper right 

hand corner, touch the Manual button to switch 

from Automatic to Manual mode operation. 

Skip this step if Manual Mode is already 

displayed. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-9

Touch the Evacuate/Start Autom. button to 

evacuate the antechamber of room atmosphere.

Wait for the gauge to settle at the negative

position and continue to evacuate for: 

Non-porous Material: 5 minutes 

Porous Material: 12 hours

Touch the Evacuate/Start Autom. button to 

deactivate the evacuation process.

Touch the Refill button to refill the antechamber 

with box gas. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-10

Non-porous Material 

Wait for the gauge to settle at the halfway 

mark on the gauge. 

Porous Material 

Wait for the gauge to settle at zero (0). 

 

Touch the Refill button to deactivate the refill 

process.

Repeat this process as necessary according the following recommended guidelines: 

Non-porous Material 

Evacuation (3 total cycles) 

• 2 Additional Cycles – 5 minutes each 

Refill (3 total cycles – 2 intermediate, 1 final) 

• 1 Intermediate Cycle – refill chamber halfway 

• 1 Final Cycle – refill chamber to atmosphere (or zero on the gauge).

Porous Material 

Evacuation 

• 1 Cycle – 12 hours 

Refill 

• 1 Cycle – refill chamber to atmosphere (or zero on the gauge).

Transferring Material from the Antechamber into the Glovebox 

Grasp the door handle with two fingers in a 

counterclockwise motion. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-11

Carefully open the antechamber door by lifting it 

in an upward direction and guiding it until it stops. 

Gently pull out the tray and transfer the material 

into the glovebox. 

At this time, materials to be transferred out of the 

box may be placed on the tray. 

After all materials are transferred into the 

glovebox, grasp the door handle and gently pull 

the door down until it rests on the door stop. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-12

Grasp the door handle with two fingers and turn in 

a clockwise motion. Tighten finger tight and then 

an additional 1⁄4 turn. 

9.5 Mini Antechamber Operation 

Operating the mini antechamber is done manually. Follow the steps below to perform a transfer 

of materials from room environment into the glovebox using the large antechamber. 

Before opening the outside antechamber door, verify: 

• The inner antechamber door is closed; 

• The hand valve (A) is in the closed position; 

• The antechamber is not under vacuum (gauge 

(B) should read zero). 

The picture to the right displays the correct hand valve 

position and the reading on the gauge at the zero 

position. 

Turn the handle on the outer antechamber door in a 

counterclockwise direction to open it. 

A 

B
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-13

Remove the door. 

Gently pull out the sliding tray. 

Place the materials to be used on the tray and slide the tray back into the antechamber.

Replace the cover and lock into place by turning in a clockwise motion.

Evacuate the antechamber by turning the hand valve 

to the Evacuate position. 

Wait for the gauge to settle at the negative position 

and continue to evacuate for: 

Non-porous Material: 3 minutes 

Porous Material: 12 hours

Refill the antechamber by turning the valve to the 

Refill position. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-14

Non-porous Material 

Wait for the gauge to settle at the halfway mark 

on the gauge. 

Porous Material 

Wait for the gauge to settle at zero (0). 

 

Repeat this process as necessary according the following recommended guidelines: 

Non-porous Material 

Evacuation (3 total cycles) 

• 2 Additional Cycles – 3 minutes each 

Refill (3 total cycles – 2 intermediate, 1 final) 

• 1 Intermediate Cycle – refill chamber halfway) 

• 1 Final Cycle – refill chamber to atmosphere (or zero on the gauge).

Porous Material 

Evacuation 

• 1 Cycle – 12 hours 

Refill 

• 1 Cycle – refill chamber to atmosphere (or zero on the gauge).

Turn the hand valve to the Closed position. 

Turn the handle on the inner antechamber door in a 

counterclockwise direction to open it. 

Remove the door. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-15

Gently pull out the sliding tray. 

Remove the materials to be used from the tray and slide the tray back into the antechamber.

Replace the cover and lock into place by turning in a clockwise motion.

Transferring Material out of the Glovebox 

Follow the above steps in reverse to transfer materials out of the glovebox into room 

environment. 

9.6 Freezers 

General Information 

The actual temperature inside the freezer will always be displayed 

even if the freezer is inactive. 

This section applies only to systems that are equipped with freezers. 

Touch the Common Parameters button on the 

Start screen.

Touch the Freezer/Box Cooling button on the 

parameters screen to display the Freezer/Box 

Cooling parameters screen. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-16

Touch the number field next to the Setpoint 

Temperature Freezer to display the alphanumeric 

keypad. Use the keypad to adjust the parameters 

in the same manner as before. 

The default setpoint is -35°C however, the freezer parameters can 

be set at any temperature between -10°C and -35°C. 

Touch the End button to return to the start screen. 

Touch the Functions button to display the 

functions screen. 

Touch the Freezer button to activate the freezer. 

Touch the End button to return to the start screen. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-17

9.7 Regeneration Mode 

The regeneration process can only be started if the circulation 

process is inactive. 

Circulation Auto Start 

If desired, the circulation process can be started automatically following the end of the 

regeneration procedure. Follow the steps below to have circulation start automatically after the 

regeneration procedure is completed. Skip this step to restart the circulation process manually 

after the regeneration procedure is completed. 

Touch the RKM (Purifier) icon on the start screen. 

Touch the Parameter button on the purifier layout 

screen. 

Select Yes from the drop down menu. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-18

Touch the End button to return to the start screen. 

Regeneration 

Follow the steps below to start the regeneration process.

Touch the Functions button on the start screen. 

Deactivate the circulation process, if active, by 

touching the Circulation Purifier button. 

Touch the Regeneration Purifier button on the 

functions screen. 

The circulation button is blanked out as this function 

is unavailable during the purging process. 
Operating Instructions – Labmaster © 130 and MB20G Systems 

Instruction Manual 

Chapter 9: System Operation 

© MBraun, Inc. Edition 1/2007 Page 9-19

The purifier layout screen is displayed with the 

following message. 

Please confirm that you wish to regenerate the 

purifier? 

This message must be acknowledged (yes or no) 

before regeneration will initialize. 

Using the regulator, turn on the regeneration gas supply and set the pressure to 5psi. 

Place the end of the regeneration vent line running from the VRS valve on the valve block 

located on the back of the purifier to a 100ml (minimum size) collection vessel. 

Vent the line and vessel to fume hood or other facility ventilation system. 

A second message is displayed: 

Prove flow of the regeneration gas! If OK Æ

Confirm this button 

This message must also be acknowledged before 

regeneration will initialize. Touch the message on 

the screen after confirming the flow is correct 

according to the above information. 

After acknowledging the second message the 

purifier layout screen can remain displayed. This 

screen will display the active step in the 

regeneration process. 

or 

Touch the End button to display the start screen. To 

display the purifier layout screen at any time during 

the regeneration process touch the RKM icon on the 

start screen. 

• After completing a regeneration process, the vacuum pump oil 

should be changed. Refer to vendor supplied manuals for 

instructions on changing the oil. 

• Used oil should be disposed of in accordance with all facility, 

local and federal guidelines. 
Chapter 10

Maintenance &

Troubleshooting

10.1 General Information....10-1

10.2 Maintenance Requirements....10-1

10.3 Touch Panel Maintenance....10-2

10.4 Replacing the Gloves....10-5

10.5 Moisture Analyzer Maintenance....10.6

10.6 Alarms....10-12
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-1

CHAPTER 10: MAINTENANCE & TROUBLESHOOTING

10.1 General Information 

This chapter provides general information for general maintenance of a single purifier glovebox 

system and troubleshooting various problems which may be encountered during operation of 

the system. For information regarding troubleshooting of vendor manufactured components, 

please consult the vendor supplied user manuals. 

Contact the M.Braun Service Department with questions 

regarding any of the troubleshooting steps listed below. 

10.2 Maintenance Requirements 

Daily Maintenance and Service 

Windows: Clean the exterior with a mild solution of soap or detergent and lukewarm 

water. 

Using a soft lint free cloth or sponge, gently wash the sheet to loosen dirt and 

grime and rinse well with clean water. 

To prevent water spotting, thoroughly dry with chamois or cellulose sponge. 

Avoid the use of abrasive cleaners, squeegees and/or other cleaning 

implements that may mar or gouge the coating. 

Compatible Cleaning Agents: 

Aqueous Solutions of Soaps and Detergents 

Windex1

 Top Job2 Joy2 

Mr. Clean2 Fantastik3 Formula 4094

1 Registered Trademark of Drackett Products Co. 

2 Registered Trademark of Procter & Gamble 

3 Registered Trademark of Texize, Division of Norton Norwich Products Inc. 

4 Registered Trademark of Clorox Co. 

Organic Solvents 

Butyl Cellosolve Hexcel, F.O. 554 

Kerosene Neleco–Placer 

Naphtha (VM&P) grade Turco 5042 

Alcohols 

Methanol and Isopropyl Alcohol 

Gloves: Check all gloves for damage. 

The surface of the gloves in which the user’s hands are placed should be 

coated lightly with talcum powder to prevent drying and brittleness. 

Replace gloves immediately if any signs of damage or wear are detected. 

Never attempt to repair gloves. 

Antechamber: Check antechamber door seals for damage and/or debris. Coat lightly, as 

needed, with high vacuum grease. 

If the antechamber doors are difficult to open or close, apply a light coating of 

white lithium grease or other lubricant to the pivot points and/or door spindle. 

Connections: Check all connections to ensure they are seated properly and are leak free. 

Components: Observe the maintenance instructions of the third-party manufactured 

components as directed in supplied manuals. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-2

Quarterly and Annual Maintenance and Service 

Quarterly Annually 

• Inspect the gloves and glove ports, replace 

as needed. 

• Inspect the fluorescent box lights, replace as 

needed. 

• Inspect the solenoid valves, replace as 

needed. 

• Leakage test. 

• Test of the functions. 

• Inspect box filters, replace as needed. 

• Replace the gloves. 

• Inspect the glove ports, replace as needed. 

• Inspect the fluorescent box lights, replace 

as needed. 

• Inspect the solenoid valves, replace as 

needed. 

• Leakage test. 

• Test of the functions. 

• Replace box filters. 

• Calibrate oxygen and moisture analyzers. 

10.3 Touch Panel Maintenance 

General Information 

By accessing the System Screen the user can: 

• Calibrate the touch panel screen 

• Clean the touch panel screen 

• Adjust the date & time settings 

Below are instructions for each function. 

Screen Calibration 

Touch the System button on the Start Screen to 

access the System Settings Screen. 

Touch the Touch Calibration button to activate 

the calibration process. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-3

Five calibration crosses are displayed in 

succession at random points on the screen. 

Follow the instructions displayed on the screen and 

touch each calibration cross as it is displayed. 

To accept the changes made during the calibration 

process touch the screen at any time. 

To reject the changes made during calibration wait 

for 30 seconds until the overlaid timer bar has 

reached zero. The system will revert back to the 

default settings. 

Upon completion of the calibration procedure, 

touch the Main or Back buttons to access the Start 

Screen and resume normal operation. 

Screen Cleaning 

Touch the System button on the Start Screen to 

access the System Settings Screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-4

Touch the Screen Cleaning button. 

All system functions are locked out for 30 seconds 

while a screen with a timer bar is displayed to allow 

the user to clean the screen without interfering with 

system processes. 

Clean the screen by applying a small amount of 

non-abrasive cleanser directly to a soft lint free 

cloth and gently wipe the screen. Do not apply the 

cleanser directly to the screen! 

Upon completion of the cleaning procedure, touch 

the Main or Back buttons to access the Start 

Screen and resume normal operation. 

Setting the Date and Time 

Touch the System button on the Start Screen to 

access the System Settings Screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-5

Touch the input field for the date and/or time to 

display an alphanumeric pad. 

Enter the correct information according to the 

instructions detailed at the beginning of Chapter 4. 

After making the appropriate changes, touch the 

Main or Back buttons to access the Start Screen 

and resume normal operation. 

10.4 Replacing the Gloves 

General Information 

M.Braun recommends replacing the gloves at regular intervals. The gloves must be changed 

upon signs of wear and tear that may or have caused a leak. 

Before replacing the gloves ensure that the glovebox is atmosphere 

is safe to breathe. If necessary purge the glovebox to fill it with 

ambient air prior to changing the gloves.

Carefully remove the two o-rings 

securing the glove in place. 

Carefully remove the glove from the 

gloveport. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-6

Slide the glove onto hand and insert arm 

into the box through the gloveport 

angling the thumb to a comfortable 

working position. 

Slide hand out of the glove and work the 

cuff of the glove into the groove in the 

gloveport closest to the glass. At this 

time the thumb position can still be 

adjusted by rotating the cuff of the glove. 

Secure the glove with two o-rings. 

Place the o-ring in the top of the 

second groove from the glass and in 

one smooth motion pull down across 

the circumference of the gloveport. 

Repeat the process for the remaining 

glove(s) and o-ring(s). 

After replacing gloves, purge the glovebox to remove any undesired 

oxygen and/or moisture. (Refer to Chapter 8 Commissioning the 

System, Section 8.4 Purging)

Gloveport Covers 

M.Braun gloveport covers, which are used to assist in replacing gloves or to block off an unused 

gloveport, are made for standard round glove ports and are available for either interior or 

exterior use. 

Internal gloveport covers allow the user to replace gloves without contaminating the glovebox 

atmosphere with room air. 

External gloveport covers allow the user to remove a glove(s) and continuing working within a 

glovebox without contaminating the glovebox atmosphere with room air. 

Contact the Service Department for further information regarding price and availability of the 

gloveport covers. 

Do not seal off all gloveports at the same time. Sealing off all 

gloveports simultaneously could cause pressure to build up inside 

the glovebox which could cause the window to crack or rupture.

10.5 Moisture Analyzer Maintenance 

General Information 

If it seems necessary to clean a moisture analyzer probe, follow the instructions carefully to 

avoid damaging the sensor probe. Prior to cleaning the analyzer, contact the M.Braun Service 

Department to determine if service by M.Braun is required or if the analyzer only requires 

cleaning. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-7

Materials Required 

• Soft absorbent lint free cloth

• Phosphoric Acid

• Distilled Water

• Personal Protective Equipment (gloves, goggles, apron)

Instructions 

Touch the Functions button on the Start Screen. 

Touch the Analyzers button to deactivate the 

analyzers. 

Remove the RJ45 plug connector from the 

analyzer. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-8

Remove clamp and analyzer. 

Place a blank KF40 cap over the pipe and secure 

in place with the clamp. 

Separate the protective cover on the probe by 

turning in a counter clockwise direction. 

Slowly and carefully remove the cover from the 

probe. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-9

Place an absorbent lint free cloth under the probe 

and moisten the probe with distilled water. 

Wrap the moistened cloth around the probe. Gently 

and slowly spin the cloth around the probe to clean 

it. 

Dry the probe in the same manner with a dry lint 

free cloth. 

Do not clean the probe by moving the cloth in 

an up and down motion. This will damage the 

coil and will render the analyzer unusable 

resulting in the need for repair by M.Braun. 

Place an absorbent lint free cloth under the probe 

and moisten the probe with Phosphoric Acid. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-10

Carefully replace the protective cover and secure in 

place by turning a clockwise direction. 

Remove KF40 clamp and blank cap. 

Carefully place the sensor end of the analyzer into 

the port on the circulation piping. 

Secure in place with the KF40 clamp. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-11

Insert the analyzer cable plug into the hole on the 

analyzer by pushing the plug into the hole gently 

until it snaps into place. 

Touch the Analyzers button on the Functions 

screen to reactivate the analyzers. 

Touch the End button to return to the Start Screen. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-12

10.6 Alarms 

General Information 

The following troubleshooting guidelines address the possible alarm warnings that the user may 

encounter during operation of the system. 

Contact MBRAUN directly with any questions that arise while 

attempting to troubleshoot the system or if an alarm message is 

displayed that is not included below. Do not attempt to resolve the 

problem. 

Circulation Gas Flow 

There is no gas flow to the experiment during circulation process. 

Check the gas supply line. 

Is there gas from the main supply line into the 

system? 

Yes – proceed to next step. 

No – turn on gas supply and continue normal 

operation. Proceed to next step if there is still no 

gas flow to the experiment. 

Check the valve activation gas supply for 

correct flow rate (between 50 and 80psi) and 

adjust, if necessary. 

Did this correct the problem? 

Yes – continue normal operation. 

No – Proceed to next step. 

Check the connector plug on each VHA & VHE 

valve to ensure it is securely tightened to the 

valve body. 

Did the valve open? 

Yes – continue normal operation 

No – contact M.Braun Service Department. 

Regeneration Gas Flow 

There is no gas flow through the system during regeneration process. 

Check the gas supply line. 

Is there gas from the main supply line into the 

system? 

Yes – proceed to next step. 

No – turn on gas supply and continue normal 

operation. Proceed to next step if there is still no 

gas through the system. 

Check the gas supply for correct flow rate 

(between 5 and 10psi) and adjust, if necessary. 

Did this correct the problem? 

Yes – continue normal operation. 

No – Proceed to next step. 

Check the connector plug on each VRE and 

VRS valve to ensure it is securely tightened to 

the valve body. 

Did the valve open? 

Yes – continue normal operation 

No – proceed to next step 

Check each VRE and VRS valve on the active 

filter for a yellow LED which indicates there is a 

signal being sent to the valve from the PLC. 

Is the light on? 

Yes, the light is on but the valve is not open and 

there is no flow to the experiment. – contact 

M.Braun Service Department. 

No – contact M.Braun Service Department. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-13

Power Supply 

The Main Power switch on the front of the system is set to the On position but the system is not 

functioning. 

Is the system plugged into the main power 

supply? 

Yes – proceed to next step 

No – Turn the main power switch to the Off 

position and plug the system in. Turn the 

switch to the On position and resume normal 

operation. Proceed to next step if this does 

not fix the problem. 

Open the electrical cabinet located inside the 

system. Check main power breakers F1. 

Reset the blown breaker. 

Did this correct the problem? 

Yes – continue normal operation 

No – contact M.Braun Service Department. 

If a breaker that has been reset blows again, contact the M.Braun 

Service Department. 

Purifier Inlet/Outlet Not Open 

One or both of the valves controlling circulation flow to and from the purifier bed fails to open. 

Possible reasons for failure are: 

• The gas pressure required to open the valve is too low; 

• The gas supply tanks are empty. 

Reset the regulator on the gas supply to 80psi. 

Did the valve open? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 

Vacuum Pump VPG1: Motor Protective Switch Activated 

The vacuum pump controlling antechamber evacuation is drawing too much current. Possible 

reasons for failure are: 

• Debris in the vacuum pump; 

• Vacuum pump oil is low or degraded; 

• Vacuum pump is defective. 

Check the vacuum pump oil level using the sight 

glass. Top off the oil level as needed. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Check the vacuum pump oil color using the sight 

glass, it should be clear. Change the oil in the 

vacuum pump. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Open the electrical cabinet and check the F4

breaker. Reset it if it is blown. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-14

Blower GB1: Motor Protective Switch Activated 

The blower has exceeded current draw limit. Possible reasons for failure are: 

• The filter(s) is clogged; 

• The blower is drawing too much amperage; 

• A power outage or power surge; 

• System restart; 

• The blower is defective. 

Check the inlet and outlet filters inside the 

glovebox. Replace or clean as needed. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Open the electrical cabinet and check the F3

breaker. Reset it if it is blown. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Deactivate circulation via the Functions menu. 

Push the Fn button on the frequency converter 

to reset it. Reactivate circulation. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 

Box Pressure Too Low 

Vacuum Pump VPG1 Switched Off 

The glovebox pressure has dropped to a level below -15mbar. Possible reasons for failure are: 

• The antechamber evacuation valve is open and the inner door is not sealed properly; 

• An open auxiliary vacuum source inside the glovebox not controlled by the PLC. 

Turn the antechamber evacuation valves to the 

closed position. Verify the inner door is sealed 

completely. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Check auxiliary vacuum sources located within 

the glovebox. Ensure all seals are seated 

properly and securely. Fix the connections as 

needed. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 

Box Pressure Too High 

The glovebox pressure has risen to a level above +15mbar. Possible reasons for failure are: 

• Entering the glovebox, using the gloves, too quickly; 

• Upper working setpoint parameter is too close to the upper working limit; 

• Auxiliary gas supply is open. 

Check the upper working setpoint parameter. 

Adjust as necessary to a limit of 10mbar or 

below. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Turn off any unused auxiliary gas supply. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 
Operating Instructions – Labmaster© 130 and MB20G Systems 

Instruction Manual 

Chapter 10: Maintenance & Troubleshooting 

© MBraun, Inc. Edition 1/2007 Page 10-15

Sensor Box Pressure Defective 

The main pressure sensor for the system has failed causing circulation, analyzers, gas in and 

gas out to be deactivated. Contact the M.Braun Service Department to obtain a replacement 

sensor. 

O2 or H2O Sensor Defective 

The oxygen or moisture analyzer is not displaying a numerical reading on the PLC screen. 

Possible reasons for failure are: 

• The cable is damaged; 

• The RJ45 connector is not inserted correctly; 

• The sensor head is damaged or defective. 

Check the RJ45 connector to ensure it is 

plugged into the analyzer properly. Adjust as 

necessary. 

Did this fix the problem? 

Yes – continue normal operation 

No – proceed to the next step. 

Unplug the RJ45 connector. Inspect the cable 

and plug for damage. Reinsert the connector 

back into the analyzer. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department. 

Freezer Motor Protective Switch Activated 

Box Cooling Motor Protective Switch Activated 

The compressor used to operate the freezer or box cooling unit has exceeded current draw 

limit. Possible reasons for failure are: 

• The compressor is drawing too much amperage; 

• A power outage or power surge; 

• System restart; 

• The compressor is defective. 

Open the electrical cabinet and check the F7

breaker (freezer) or F8 breaker (box cooling). 

Reset it if it is blown. 

Did this fix the problem? 

Yes – continue normal operation 

No – contact the M.Braun Service Department.