Polarizing Microscope 

ECLIPSE E600 POL 

Instructions 
I Thank you for purchasing the Nikon products. 

This instruction manual is written for the users of the Nikon Polarizing Microscope 

ECLIPSE E600 POL. 

To ensure correct usage. read this manual carefully before operating the instrument. 

• It is prohibited to reproduce or transmit this manual in part or whole without Nikon·s 

expressed permission. 

• The contents of this manual are subject to change without notice. 

• Some of the products described in this manual may not be included in the set you 

have purchased. 

• Although every effort has been made to ensure the accuracy of this manual. if you 

note any points that are unclear or incorrect. contact your nearest Nikon 

representative. 

Warning/Caution Symbols Used in This Manual 

Although Nikon products are designed to provide you with the utmost safety during 

use. incorrect usage or disregard of the instructions can cause personal injury or 

property damage. For your safety. read the instruction manual carefully and thoroughly 

before using the instrument. Do not discard this manual but keep it near the product for 

easy reference. 

In this manual. safety instructions are indicated with the symbols shown below. Be 

sure to follow the instructions indicated with these symbols to ensure correct and safe 

operation. 

Symbol 

&WARNING 

&CAUTION 

Meaning 

Disregarding instructions marked with this symbol may lead 

to death or serious injury. 

Disregarding instructions marked with this symbol may lead 

to injury or property damage. 

Meaning of Symbols Used on the Equipment 

Symbol Meaning 

Caution for heat. 

This marking near the lamphouse calls your attention on the 

following: 

• Lamphouse becomes very hot during and immediately 

after the illumination. 

• Risk of burns. Do not touch the lamphouse during and 

immediately after the illumination. 

• Make sure that the lamphouse is sufficiently cool before 

the lamp replacement. 
&WARNING 

2 

1. Intended product use 

This microscope should only be used for microscopic observation. Do not use it for any 

other purpose. 

2. Do not disassemble 

Disassembling may cause malfunction and/or electrical shock. Do not disassemhle any 

pans other than those mentioned in this manual. If you notice any malfunction. notify your 

nearest Nikon representative. 

3. Input voltage 

Make sure that the input voltage indicated on 

the back panel of the microscope is the same 

as your regional voltage. If not the same. do 

not use the microscope; instead. notify your 

nearest Nikon representative immediately. If 

the microscope is used with the wrong input 

voltage. a short circuit or fire may result. 

causing the damage of the microscope. 

4. Power cord 

To prevent electrical shock. always turn off 

the power switch (flip it to the" 0" side) 

before connecting or disconnecting the power 

cord. Use only the following power supply 

cord set. Using the wrong power cord could 

result in damage or fire. 

• For I OO-I 20V area 

UL Listed. detachable cord set. 3 conductor 

grounding type SVT, No. 18 AWG rated at 

125V, 7A minimum. 

Nllton MODEL ECLIPSE E600 POL 

430001 

In case of using the extension cord. use only the power supply cord with the PE (protective 

earth) wire. 

• For 220-240V area 

3 pole power supply cord set. which must be approved according to EU/EN standards. . 

Class I equipment should be connected to PE (protective earth) terminal. 

In case of using the extension cord. use only the power supply cord with the PE (protective 

earth) wire. 

5. Heat from the light source 

The lamp and the lamphouse become 

extremely hot. To avoid burns. do not touch 

the lamphouse while the lamp is lit or for 

thirty minutes after it has been turned off. 

Furthermore. in order to avoid the risk of fire. 

do not place fabric, paper or highly 

flammable materials such as gasoline, 

petroleum benzine. paint thinner or alcohol 

near the lamphouse while it is lit or for about 

thirty minutes after it has been turned off. 

The back of the microscope also becomes hot 

during use. Although this is not a 

malfunction. be careful not to touch the back 

of the microscope when it is hot. 
&CAUTION 

1. Only use the specified halogen lamp 

The power supply unit in the microscope provides the power for the halogen lamp that 

serves as the microscope light source. This unit can supply power for a halogen lamp of up 

to 12V-IOOW. The specified lamp and lamphouse must be used. otherwise. the microscope 

could be damaged. 

Specified lamphouse (for diascopic use): 

Nikon C-LP HALOGEN 12VIOOW model 

Specified lamphouse (for episcopic use): 

Nikon LHS-HIOOP-2 HALOGEN 12VIOOW model 

Specified lamp (for both diascopic and episcopic uses): 

I 2V- I OOW LONG LIFE halogen lamp (OS RAM HLXM62.~ or 

PHILIPS 7724 l 

2. Precautions for replacing the lamp 

To prevent burns. allow the lamp to cool for at least thirty minutes after turning off the 

power switch before replacing it. Furthermore. to prevent electrical shock. and damage to 

the microscope. always turn off the power switch (flip it to the·· ::_: ·· side) and unplug the 

power cord before connecting or disconnecting the lamphouse. 

After replacing the lamp. he sure to attach the lamphouse cover securely. Never use the 

lamphouse without its cover. 

3. Do not wet the microscope 

If the microscope gets wet. a short circuit may result that could damage it or make it 

extremely hot. If you accidentally spill a liquid on the microscope. immediately turn off 

the power switch (flip it to the" 0" side) and unplug the power cord. Then use a dry 

cloth to wipe away the moisture. If any liquid gets inside the microscope. do not use it: 

instead. notify your nearest Nikon representative. 

4. Weak electromagnetic waves 

This microscope emits weak electromagnetic waves. The accuracy of any precision 

electronic equipment may he adversely affected if positioned too close. If the microscope 

affects TV or radio reception. move the radio or TV further away from the microscope. 

5. Precautions for installation 

Be careful not to pinch your hands or fingers when installing the microscope. 

This microscope is a precision optical instrument. and using or storing it under unsuitable 

conditions may damage it or may have an adverse effect on its accuracy. See "Installation 

location" on p. 32 and always use the microscope in a suitable environment. 

6. Precautions for moving the microscope 

First detach the lamphouse. and then securely 

hold the microscope hy the hase of the arm 

from the back. 

Do not grasp the focus knobs. eyepiece tube. 

stage. etc. when carrying the microscope. 

This could cause the part to come off. 

resulling in damage to the microscope. 

This microscope is a precision optical 

instrument. so handle it carefully and do not 

subject it to a strong physical shock. (The 

accuracy of the ohjective in particular may be 

adversely affected by even a weak physical 

shock.) 

3 
CONTENTS 

Warning/Caution Symbols Used in This Manual ......................................................... 1 

& WARNING ................................................................................................................... 2 

& CAUTION .................................................................................................................... 3 

D Names of Component Parts and Operational Parts ............................................. 6 

Iii Microscopic Procedure ........................................................................................... 8 

D Diascopic Illumination Microscopy .................................................................................. 8 

El Episcopic Illumination Microscopy ................................................................................. I 3 

II Orthoscopic Observation/Conoscopic Observation ........................................................ I 7 

D Operation of Each Part ......................................................................................... 18 

D Coarse and fine focus knobs ..... .. ... . .. ... ... ... .. . ....... ....... ............ .. . ... . . . . . . .. . . .. . . . . . . . . . .. .. . .. . .. . . . . 18 

I. Rotation of the coarse and fine focus knobs and vertical movement of the stage .... 18 

2. Adjusting the torque of the coarse focus knob ........................................................... 18 

3. Coarse focus stopper ................................................................................................... 19 

El Trinocular eyepiece tube .................................................................................................. 20 

1. Optical path selection ................................................................................................. 20 

2. Vertical tube adapter ................................................................................................... 20 

3. Optical path selection lever clicking .......................................................................... 20 

II Diopter adjustment ........................................................................................................... 21 

D Interpupillary distance adjustment ................................................................................... 21 

II Condenser ......................................................................................................................... 22 

1. Focusing and centering ............................................................................................... 22 

2. Condenser aperture diaphragm ................................................................................... 23 

3. Swing-out the top lens of the condenser .................................................................... 24 

Iii Field diaphragm ............................................................................................................... 24 

I.I Filters ................................................................................................................................ 24 

Iii Auto-photo switch (for photomicrography) .................................................................... 25 

Iii Oil-immersion operation (using an oil-immersion type of objective and condenser) .... 25 

mJ Orientation of polarizing plates (Polarizer and Analyzer) .........................................•.... 26 

m Focusing and centering the Benrand lens ........................................................................ 28 

18 P-CL l/4A. & tint plate ..................................................................................................... 28 

Ill Centering the objectives ................................................................................................... 28 

ID Circular graduated stage for polarizing microscope ........................................................ 29 

ml Use of optional accessories (Sold Separately) ................................................................. 29 

1. P-CS Senarmont Compensator ................................................................................... 29 

'"' P-CQ Quartz wedge .................................................................................................... 30 

3. Attachable Mechanical Stage ..................................................................................... 31 

III Assembly ............................................................................................................... 32 

~Troubleshooting Tables ........................................................................................ 41 

n Care and Maintenance .......................................................................................... 45 

H Specifications and Standards .............................................................................. 46 

5 
Names of Component Parts 

and Operational Parts 

If the microscope has not yet been assembled. see chapter .. 4. Assembly'" first. 

Polarizing 

intermediate tube 

Bertrand lens 

centering screw 

45° click-stop lever 

screw 

Coarse torque adjustment ring (TORQUE) 

Coarse focus knob 

Fine focus knob 

Brightness adjuster 

(LAMP) 

Auto-photo switch 

Auto-photo voltage 

selection switch 

6 

Photomicrographic 

vertical tube adapter 

Trinocular 

eyepiecetube 

Eyepiece 

Diopter 

adjustment ring 

Analyzer slider 

P-CL 1 /4'A & tint plate 

Revolving nosepiece 

Objective centering screws 

Objective 

Stage rotation clamp screw 

Vernier 

Specimen clip 

Circular graduated stage 

Dia-polarizer 

(Inside lhe bottom of !he condenser) 
Bertrand lens turret 

Bertrand lens focus 

ring 

Condenser top lens 

swing-out knob 

Condenser aperture 

scale 

Condenser aperture 

diaphragm ring 

Swing-out condenser 

Condenser centering 

screws 

Field lens 

Field diaphragm ring 

Epi/Dia changeover switch 

Tool holder 

(Holds a hexagonal screWdnver. 

a hexagonal wrench and two centenng screws.) 

AC input connector 

Power cord 

1. Names of Component Parts and Operational Parts 1' 

(Rear) 

Photomicrographic 

equipment clamp screw 

Optical path 

selection lever 

Eyepiece tube 

clamp screw 

Intermediate tube 

clamp screw 

Revolving nosepiece 

clamp screw 

Substage clamp scr~vv 

Filter release lever 

Diffuser insertion/ 

removal screw 

Epi-illuminator connector 

Rating label 

Lamphouse 

7 
8 

Microscopic Procedure 

The general procedure for microscopy is described below. 

For details on each step. refer to the corresponding item in chapter .. 3. Operation of Each Part." 

If the microscope has not yet been assembled. see chapter "4. Assembly .. first. 

1 Diascopic Illumination Microscopy 

D Turn the Epi/Dia changeover 

switch on the back of the 

microscope stand to the DIA 

position. 

a Turn on the power. 

Kii:I (Flip the switch to the .. I •. side.) 

As soon as the power comes on. 

the switch lights. 

1:'11 Set the brightness adjuster to 

a give the desired brightness. 

Pressing the auto-photo switch 

will set the voltage for the 

brightness that provides the best 

color reproduction. (p.25) 

B Insert filters ND32 or 8 and 

U NCB 11 into the optical path. 

ND32 or 8 reduces glare in the 

binocular eyepiece. NCB 11 

improves color reproduction. 

(p.24) 

a Push in the analyzer slider of the 

a intermediate tube to remove the 

~ivi:v, analyzer from the optical path. ··~· i'·.·.;.··~;. 

;.:i~j: 

1'!!9 Put the Bertrand lens turret in the 

a .. 0 .. position to remove the 

Bertrand lens from the optical 

path. 

II Insert filters. ,_,~ 

II Adjust the brightness. ~ ·~,~ Auto-photo switch can be pressed _! 

1-11 Push in the 

, analyzer 

/ 

~' -1'1 Turn the turret to 

the "O" position. 

slider. 
n Move the !OX objective into the 

.. optical path. 

Rotate the revolving nosepiece 

,,, until the !Ox objective clicks 

into place. 

n Set the specimen in place with 

... the cover glass facing up. 

Raise the condenser as high as it 

will go. 

rr.i. Fully open the field diaphragm 

~ and condenser apenure 

diaphragm. 

II Set the 

2. Microscopic Procedure I 

-D Set the 10x 

objective. 

---

~ U!J Fully open the 

field diaphragm. 

9 
10 

m ~oosen the coarse focus stopper 

nng. 

Rotate it in the direction opposite 

the arrow on the base as far as it 

will go. (p.19) 

m Set t~e optical path to I 00</t of 

lliii:ll the bmocular eyepiece when 

· · using the trinocular eyepiece 

tube. (p.20) 

m Turn the diopter adjustment ring 

11iii1 of the right eyepiece (containing 

the crosshairs) and focus on the 

crosshairs. 

Focus on the specimen. (p.18) 

I I I 

, I i $] 

m Loosen the coarse focus stopper. 

!: 

DJ Focus on the 

reticle crosshairs. 

Im Set the optical path to 

100% of the binocular 

eyepiece. 

ID Focu~ on the specimen. 
m Adjust the diopter . f o the left 

lli:il eyepiece. (p.2 I) 

1!P-9 Adjust the m · terpupillary 

ll:il distance. (P-21 ) 

. . (p 28) m Center the objecuve. · 

m Focus and center the condenser. 

.... (p.22) 

\ / 

v . ID Center the objective. 

2. Microscopic · p ro cedure I 

_ Im Adjust the 

diopter nng. 

Im Adjust the interpupillary 

distance. 

h ondenser. Im Center t e c =-------

11 
12 

m Switch to any desired objective 

l&;I and view the specimen. 

j,.. Rotate the revolving nosepiece to 

its click-stop position. 

In the case of using an oilimmersion objective. apply oil 

while being careful that air 

bubbles do not form between the 

end of the objective and the 

specimen. 

Manipulate the coarse focus 

knob or fine focus knob to refocus on the specimen. 

Manipulate the ND filter 

insertion lever to adjust the 

brightness. 

(When adjusting the lamp 

brightness. turn off the autophoto switch and manipulate the 

brightness adjuster.) 

Close the field diaphragm so that 

it is just outside the field of view. 

(p.24) 

Close the condenser aperture 

diaphragm to about 70 - 80% of 

the numerical aperture of the 

objective. (p.23) 

BJ Switch the objective. 

m Adjust the 

brightness 

with ND filters. 

Sil Readjust the focus. 

Pa Adjust the field diaphragm. 

Pa Adjust the condenser 

aperture diaphragm. 
2 

2. Microscopic Procedure I 

Episcopic Illumination Microscopy 

D 

~ ~ .. 

B 

EJ 

II 

g 

m 

Push in the illumination 

changeover lever of the Epiilluminator to the limit 

(indication: B.F) to set the 

brightfield illumination. 

Turn the Epi/Dia changeover 

switch on the back of the 

microscope stand to the EPI 

position. 

Turn on the power. (Flip the 

switch to the·· I" side.) 

As soon as the power comes on. 

the switch lights. 

Set the brightness adjuster to 

give the desired brightness. 

Pressing the auto-photo switch 

will set the voltage for the 

brightness that provides the best 

color reproduction. 

Insert filters ND2 or 4 and 

NCB 11 of the Epi-illuminator 

into the optical path. 

ND2 or 4 reduces glare in the 

binocular eyepiece. NCB 11 

improves color reproduction. 

Confirm that the ND8 anti-glare 

filter lever is pushed in. 

Push in the analyzer slider of the 

intermediate tube to remove the 

analyzer from the optical path. 

Put the Bertrand lens turret in the 

"O'' position to remove the 

Bertrand lens from the optical 

path. 

D Set the brightfield illumination. 

•• .\=' lij ~ f: -1 _Jj)_J 

((_"°'+ii~ ~:---- ----~ fJ Flip the switch " ~oc::_l to the "EPI" side. 

II Adjust the 

brightness. 

0

i't - , 

1

11 Flip the switch 

to the .. I " side . ...-

'C. I 

:"'~ '~ 

,-61 Push in the 

analyzer slider. 

- Iii Push in the NOS 

filter lever. 

- l!I Turn the turret to the "O" position. 

13 
14 

n Move the lOX objective into the 

a optical path. 

" ,,, · Rotate the revolving nosepiece 

~· until the I Ox objective clicks 

:=.~ into place. 

!l:'t Set the specimen in place with 

IA:. the cover glass facing up. 

m Fully open the field diaphragm 

and aperture diaphragm of the 

Epi-illuminator. 

m ~oosen the coarse focus stopper 

lrii:I rmg. 

· · Rotate it in the direction opposite 

the arrow on the base as far as it 

will go. 

!1:1 Set the optical path to I 00% of 

1£.1 the binocular eyepiece when 

~~, ~::~ the trinocular eyepiece 

Im Set the 

specimen. 

-II Set the 1 Ox objective. 

'i: 

I, 

11 

--

I I'~~ 

...... 
Turn the diopter adjustment ring 

of the right eyepiece (containing 

the crosshairs) and focus on the 

crosshairs. 

IE Focus on the specimen. 

Adjust the diopter of the left 

eyepiece. 

~ Adjust the interpupillary 

Ill distance. 

m Center the field diaphragm of the 

11:.i Epi-illuminator. 

Im Center the objective. 

2. Microscopic Procedure I 

eJ Focus on the 

reticle crosshairs. 

ID Adjust the inter- . 

pupillary distance. 

Im Focus on the specimen. 

IIiJ Center the 

objective. 

111J Center the field 

diaphragm. 

,.----

r ~--

I 

15 
16 

~.:i4;~~­

f,:h4r~. 

0\j:}tf'. 

Switch to any desired objective 

and view the specimen. 

Rotate the revolving nosepiece to 

its click-stop position. 

In the case of using an oilimmersion objective, apply oil 

while being careful that air 

bubbles do not form between the 

end of the objective and the 

specimen. 

Manipulate the coarse focus 

knob or fine focus knob to refocus on the specimen. 

Adjust the brightness using the 

ND filter of the Epi-illuminator. 

(When adjusting the lamp 

brightness, turn off the autophoto switch and manipulate the 

brightness adjuster.) 

Adjust the field and aperture 

diaphragms of the Epiill uminator. 

Field diaphragm: 

Close it to the size just 

outside the field of view. 

Aperture diaphragm: 

Close it to the size about 

70 - 809C of the numerical 

aperture of the objective. 

ml Switch the objective. 

fD Readjust the focus. 

~f!J Adjust the field diaphragm. 

:! ,f!] Adjust the condenser 

I aperture diaphragm. 

This completes the procedure for microscopy for normal observation. Refer to the following 

section 3 for details regarding the microscopy procedures for "Orthoscopic Observation" and 

"Conoscopic Observation". 
2. Microscopic Procedure I 

3 Orthoscopic Observation/Conoscopic Observation 

The following provides an explanation of the characteristic observation method of polarizing microscope~ 

along with the microscopy procedure. If normal microscopy has not yet been completed. refer to the 

previous section I or 2 and complete normal microscopy. 

Orthoscopic Observation 

• In this method. the specimen is observed with the 

polarizer and analyzer placed in the optical path. 

•Operation 

Pull out the analyzer slider and move the analyzer 

into the optical path. 

• In this case. the shape of the specimen is visible 

(direction of optical axis) and the optical properties 

relative to the direction of the thickness of the 

specimen can be observed. 

Conoscopic Observation 

• In this method. in addition to the polarizer and 

analyzer. the Bertrand lens is also moved into the 

optical path when observing a specimen. 

•Operation 

Move the analyzer into the optical path. and place the 

Bertrand lens turret of the intermediate tube in 

position "B" to move the Bertrand lens into the 

optical path. (Refer to p.28 for details regarding the 

focusing procedure.) Place the P-CL 1/4/.. & tint 

plate in the hollow position. Use an objective having 

a large numerical aperture (high magnification: 

normally 40 or higher). 

Analyzer in the optical path 

[]] 

Analyzer and Bertrand lens 

in the optical path 

• Specimens can be observed from various angles with diascopic light in the form of a single image. 

Differing from orthoscopic observation. however. the shape of the specimen itself is not visible 

with this observation. 

The settings of each part in orthoscopic microscopy and conoscopic microscopy are summarized below. 

Orthoscopic Observation Conoscopic Observation 

Purpose of To investigate the vibration direction and birefringence To distinguish between umax1al and biaxial 

observation of observation light by observing the extinction and properties and observe the optic-axial angle 

interference color due to the specimen. and optical characteristics in minerals. 

Top lens of 1 Ox or higher IN 

the condenser 4x or lower OUT IN 

Benrand lens OUT <Turret position: 0) IN ITurret position: Bl 

Aperture 1 Ox or higher 70 - 80'k of the numerical aperture Circumscribed the circumference of the of the objective diaphragm 4x or lower Fully opened conoscopic field of view (or fully opened) 

Field 1 Ox or higher Circumscribed the circumference of Circumscribed the circumference of the the eyepiece field of view 

diaphragm 4X or lower Fully opened orthoscopic field of view 

17 
18 

Operation of Each Part 

D Coarse and fine focus knobs ,;:d~ 

: • 1. Rotation of the coarse and fine focus knobs and 

vertical movement of the stage ••••••••••••••••• 

Rotating the fine focus knob one step moves the stage 

1 μm. 

Rotating the fine focus knob one complete turn moves 

the stage 0.1 mm. 

Rotating the coarse focus knob one complete turn moves 

the stage 12 mm. 

The coarse/fine focus stroke (range of vertical motion) 

for the stage is 2 mm up and 23 mm down from the 

reference (focused) position. 

Never attempt either of the following actions. These 

actions will damage the microscope. 

• Rotating the left and right knobs in opposite 

directions at the same time. 

• Continuing to rotate the coarse focus knob after the 

stage has reached the limit of its motion. 

Adjusting the torque of the coarse focus knob 

It is possible to adjust the torque of the coarse focus 

knob. 

To increase the torque. turn the torque adjustment ring 

(TORQUE) located behind the coarse focus knob in the 

direction of the arrow on the microscope base (i.e .. the 

counter-clockwise direction). To reduce the torque. turn 

the ring in the direction opposite to the arrow (i.e .. the 

clockwise direction). 

i !: 

Direction of coarse/fine focus knobs 

and stage vertical motion 
3. Operation of Each Part I 

• 3. Coarse focus stopper 

Clamp the coarse 

focus stopper 

Purpose of the coarse focus stopper 

The coarse focus stopper marks the stage position at which the specimen is in focus by 

restricting the movement of the coarse focus knob. (Movement of the stage by the fine focus 

knob is not restricted.) 

Once the coarse focus stopper has been clamped in position. the coarse focus knob cannot be 

used to move the stage any higher. In effect. once the coarse focus knob has been clamped in 

place at the focus position. a rough focus can be attained the next time simply by turning the 

coarse focus knob as far as it will go. This feature is convenient when viewing similar 

specimens one after another. 

Using the coarse focus stopper 

With the specimen in focus. turn the coarse focus stopper ring as far as it will go in the direction 

of the arrow on the base of the microscope (about 3/4 revolution). The coarse focus stopper is 

now clamped in position. 

When changing the specimen. lower the stage by turning only the coarse focus knob. 

After changing the specimen, gently raise the stage by turning only the coarse focus knob as far 

as it will go. 

The specimen should be roughly in focus when the stage has been raised as far as it will go: use 

the fine focus knob to bring the specimen into perfect focus. 

If the coarse focus stopper is not being used, be sure to turn the coarse focus stopper ring in the 

direction opposite to the arrow on the microscope base as far as it will go. 

19 
20 

El Trinocular eyepiece tube 

~?fj 1. Optical path selection 

The optical path selection lever can be used to select the way to divide the amount of light 

between the binocular part and the vertical tube. 

·.< ·'· >'.:!':/'"· 

Lever 

position 

I 

::?:: 

:I 

'.<··: ~-.:· ': 

U~· 2. Vertical tube adapter 

A photomicrographic vertical tube adapter is provided as 

standard equipment that allows a photomicrographic 

equipment to be installed. To install the adapter. insert 

it into the vertical tube and clamp three screws with the 

provided screwdriver. Replace this adapter with the 

optional TV vertical tube adapter when using a TV 

camera. 

~:jj 3. Optical path selection lever clicking 

There is a switch identified by "NO CLICK" on the 

bottom surface of the eyepiece tube. Tum the switch in 

the direction of the arrow with the hexagonal 

screwdriver provided to disengage the clicking action of 

the optical path selection lever. Disengaging the 

clicking action minimizes small vibrations produced by 

operating the lever. 

Light proportion 

binocular vertical 

part tube 

100 0 

20 

0 

80 

100 
IJ Diopter adjustment 

The diopter difference between both eyes is adjusted by 

adjusting the diopter. In the case of a polarizing 

microscope. since an eyepiece containing crosshairs is 

used for the right eye. the procedure for adjusting the 

diopter differs from that of an ordinary microscope. 

To begin with, turn the diopter adjustment ring on the 

right eyepiece to bring the crosshairs in the eyepiece into 

focus clJ. Next, focus on a specimen on the stage while 

viewing with the right eye. and then turn the diopter 

adjustment ring on the left eyepiece 21 and bring the 

specimen into focus. 

IJ 1nterpupillary distance adjustment 

Before adjusting the interpupillary distance, perform 

steps D to ii in chapter "2-1. Diascopic Illumination 

Microscopy" so that the specimen is in focus with the 

!OX objective. (Perform steps D to Im in 

"2-2. Episcopic Illumination Microscopy".) 

Adjust the interpupillary distance so that the view field 

for each eye is at the same position on the specimen. 

Doing so will make observation through the binocular 

eyepiece with both eyes easier. 

3. Operation of Each Part I 

i~ 

I 

21 
22 

IJ Condenser 

Focusing and centering 

Image of field diaphragm 

Focus and center the condenser so that the light passing through the condenser forms the image at 

the correct position on the specimen (i.e .. at the center of the optical path). 

Before focusing and centering the condenser, perform steps D to IIiJ in chapter "2-1. Diascopic 

Illumination Microscopy" so that the specimen is in focus with the lOX objective. 

1 Close the field diaphragm to its minimum setting. 

2 Turn the condenser focus knob so that the image of the field diaphragm is formed on the 

specimen. 

3 Make rough adjustments with the condenser centering screws so that the image of the field 

diaphragm appears at the center of the eyepiece view field. 

4 Move the 40x objective into the optical path. Turn the fine focus knob to focus on the 

specimen. 

5 Turn the condenser focus knob so that the image of the field diaphragm is formed on the 

specimen. 

6 Adjust the condenser centering screws so that the image of the field diaphragm appears at 

the center of the eyepiece view field. This adjustment is easier to make if you adjust the 

size of the field diaphragm to be slightly smaller than the eyepiece view field. 
!; • 2. Condenser aperture diaphragm 

Numerical 

Aperture 

I 

40XJ(Q~~)p 

co/0.17 WD 0.65 

0.65 x 0.7 - 0.8 = 0.455 - 0.52 

• Orthoscopic Microscopy 

3. Operation of Each Part I 

The aperture diaphragm is important because it is related to the resolution. contrast. depth of focus 

and brightness of the optical image. Turning the condenser aperture diaphragm ring changes the 

size of the aperture diaphragm. 

As the aperture diaphragm is stopped down. resolution and brightness arc reduced while contrast 

and depth of focus are increased. Conversely. as the aperture diaphragm is opened. resolution and 

brightness are increased while contrast and depth of focus are reduced. It is not possible to adjust 

one pair of characteristics without affecting the other. Generally. a satisfactory image with 

appropriate contrast can be obtained with an aperture setting that is 70</1c to 809c of the numerical 

aperture of the objective. The numerical aperture is indicated on the barrel of each objective. 

An indication of 40X/0.65 means that the magnification is 40x and the numerical aperture is 

0.65. 

If the aperture diaphragm is stopped down too far. the resolution is reduced: therefore, except 

when viewing a nearly transparent specimen. we do not recommend stopping down the aperture to 

less than 60'7c of the numerical aperture of the objective. 

I Adjusting the size of the aperture diaphragm according to the condenser scale 

Since the condenser scale indicates the numerical aperture. adjust the aperture diaphragm ring 

according to the scale. (Normally. the index on the aperture diaphragm ring should be aligned 

with the scale line corresponding to 70% to 80% of the numerical aperture of the objective.) 

I 

Adjusting the size of the aperture diaphragm using the Bertrand lens 

Insert the Bertrand lens into the optical path (by placing in position "B''). Turn the diaphragm 

control ring to stop down the aperture diaphragm to its minimum setting. Turn the Bertrand 

lens focus ring to focus on the aperture diaphragm. Turn the diaphragm control ring to adjust 

the aperture diaphragm. (This is normally adjusted to 70-80% of the field of view.) 

• Conoscopic Microscopy 

In the case of conoscopic microscopy. the condenser aperture diaphragm functions as a field 

diaphragm on the conoscopic image surface. Stop down the diaphragm until it circumscribes the 

circumference of the field of view of the conoscopic image (pupil of the objective). 

23 
24 

, Top lens 

"' - Swing-out knob The rop lens of rhe swing-out condenser can be moved 

outside the optical path with the swing-out knob. 

During normal orthoscopic microscopy or conoscopic 

microscopy. the top lens is used while positioned in 

the oprical palh. During orthoscopic microscopy 

using a low-power objecrive of 4X or lower. swing out 

the top lens. 

~~-- ';;: . 

During measurement of retardation or evaluation by 

interference color. swing out the top lens (the 

condenser aperture diaphragm may be stopped down) 

and illuminate with light that is as parallel to the 

optical axis as possible. 

II Field diaphragm 

The field diaphragm restricts illumination to the area on the specimen being viewed. Turning the 

field diaphragm ring changes the size of the field diaphragm. For normal observation. the size of 

the diaphragm should be slightly larger than the boundary of the view field. If a broader area than 

necessary is illuminated. stray light will enter the view field, creating flaring and reducing the 

contrast of the optical image. The correct setting of the field diaphragm is especially important in 

photomicrography: generally, good results are obtained by stopping down the field diaphragm to 

just slightly larger than the area that will be reproduced on the film. i.e .. the size of the photo 

frame. 

B Filters 

Three filters are housed in the base of the microscope. 

A filter is inserted into the optical path by pressing in the filter insertion lever on the right side of 

the microscope. The filter is removed from the optical path by pressing the release lever down. 

NCB11 (color balancing filter) For color balance adjustment and color photomicrography 

ND32 (transmission rate: 3%) For brightness adjustment 

NOB (transmission rate: 12.5%) For brightness adjustment 

GIF (Optional) For retardation measurement and contrast adjustment 

(Place on the field lens.) 

A diffuser is contained in the microscope. When removing the diffuser from the optical path, turn 

the diffuser insertion/removal screw as far as it will go (about 90°) in the counter-clockwise 

direction with a hexagonal screwdriver. When returning the diffuser to the optical path, tum the 

screw as far as it will go in the clockwise direction. 
3. Operation of Each Part I 

m Auto-photo switch (for photomicrography) 

The color temperature of the lamp varies according to the voltage. If the voltage is high. the color 

temperature of the lamp increases and the light becomes bluer. If the voltage is low. the color 

temperature of the lamp decreases and the light becomes redder. Therefore. to obtain the best 

color reproduction in color photomicrography. it is necessary for the lamp voltage to he kept 

constant. When using daylight-type color film. the standard setup is to use the color balancing 

filter (NCB 11 land set the lamp voltage to 9 V. 

The auto-photo switch is used to automatically set the standard lamp voltage (9 V l. If the images 

on color film shot with the auto-photo switch on are 

reddish or bluish. finely adjust the voltage with the autophoto voltage selection switch. The center position of the 

5-level slide switch is roughly 9 V. Sliding the switch 

forward increases the bluish tint of the light. while sliding 

the switch towards the hack increases the reddish tint of 

the light. 

Use commercially available color compensation filters 

(CC filters) if this adjustment does not resolve the 

problem. 

m Oil-immersion operation 

(using an oil-immersion type of objective and condenser) 

An objective marked "Oil" is an oil-immersion type. 

These objectives are used with the immersion oil applied 

between the specimen and the tip of the objective. The 

immersion oil is provided for the microscope. 

Bubbles in the oil will adversely affect the viewing of 

the image. so he careful to prevent the formation of air 

bubbles. To check for air bubbles, remove the 

eyepieces. fully open the field and aperture diaphragms. 

and look at the exit pupil of the objective within the eyepiece tube. (The exit pupil will appear as 

a bright circle.) When it is difficult to see if there are any bubbles. mount a centering telescope 

(sold separately) on the eyepiece sleeve with an adapter (sold separately). Then. while turning the 

eyepiece on the centering telescope to change the focus. look through the centering telescope for 

air bubbles. If there are bubbles in the oil. remove them by one of the following methods: 

• Turn the revolving nosepiece slightly, moving the oil-immersed objective back and forth once 

or twice. 

• Add more oil. 

• Remove the oil and replace it with new oil. 

Use as little oil as possible Uust enough to fill the space between the tip of the objective and the 

specimen). If too much oil is applied, the excess will flow onto the stage. 

25 
26 

Any oil remaining on an oil-immersion type of objective or staining on the tip of a dry type of 

objective has a negative effect on viewing. After using oil. wipe all of it away and make sure that 

there is no oil on the tips of the other objectives. 

Use petroleum benzine to wipe away immersion oil. Removing the oil and wiping with absolute 

alcohol (ethyl alcohol or methyl alcohol) will complete cleaning. 

If you cannot obtain petroleum benzine, use methyl alcohol. Note that methyl alcohol does not 

clean as well as petroleum benzine and it will be necessary to repeatedly wipe the surfaces (three 

or four times is usually sufficient to clean the lenses.) 

&WARNING When using petroleum benzine or absolute alcohol. always follow the 

instructions provided by the manufacturer. Keep these flammable liquids away 

from fire or sparks. 

II!] Orientation of polarizing plates (Polarizer and Analyzer) 

; • Diascopic Observation 

The dia-polarizer and analyzer coincide with the directions of the orientation plate on the top of 

the microscope base when the protractor scale is at ''O'' (the polarizer is in the X direction: P and 

the analyzer is in the Y direction: A), and are in the crossed Nicols position. The dark cross image 

as shown in the diagram can be observed at this time in the conoscopic observation. 

It should be noted that the X direction is explained as that of the analyzer and Y direction 

as that of the polarizer in some commercially available technical manuals and reference 

books. 

Polarizer 

scale: O 

The dia-polarizer is attached to the bottom of the 

substage and can be rotated 360°. It can be removed 

from the bottom of the substage by pulling down as 

necessary. When re-attaching. align the positioning 

pin on the top of the polarizer with the groove in the 

bottom of the substage. Since the groove in the 

bottom of the substage is not visible when the 

condenser is attached to the substage, attach the 

polarizer after temporarily removing the condenser. 

Analyzer Dark 

scale: O cross image 
3. Operation of Each Part I 

The analyzer can be rotated hy first loosening the 

analyzer clamp screw i]J and then turning the rotation 

dial 2;. The angle of rotation can be read to 0.1 

degrees over a range of 0-360 degrees with the 

vernier. 

The analyzer can be moved out of the optical path by 

pushing in the analyzer slider .1..,. Although the 

analyzer is designed. as standard. to be inserted from 

the right relative to the intermediate tube. it can also 

be inserted from the left. In this case. it may be 

somewhat difficult to read the angle of rotation since 

the readings on the scale. etc. are backward. 

Since the intermediate tube contains a built-in depolarizer. it is not necessary to he 

concerned about the relationship between the orientation of the polarizing plate and 

photomicrographic devices. 

• Episcopic Observation 

- First click position 

- Second click position 

In the case of performing episcopic polarizing 

microscopy. an episcopic polarizer is used in 

combination with the analyzer of the intermediate tube. 

Place a mirror or other isotropic specimen having a high 

reflectance on the stage and focus on that specimen. Set 

the analyzer scale to "ff' and replace the hollow slider of 

the epi-illuminator with the polarizer slider and insert 

the polarizer slider into the epi-illuminator. The empty 

hole will enter the optical path when the polarizer slider 

is pushed in until it clicks once. 

= 

..,,~..., =t~-----' ·: - .- ·~ '.: ,: ~J_CT~~ -- -' _ , Polarizer 

--it!'-- =:..-:~r?tation 

The polarizer will enter the optical path when the 

polarizer slider is push in until it clicks twice. 

Turn the polarizer rotation ring of the polarizer slider 

until it reaches the crossed Nicols position in the same 

manner as during diascopic observation. 

4 _::,.,,. _c:c. nng ~\\•' ·, -:__.---· 

~ Polarizer slider 

Direction of 

polarizer 

I Lateral 

direction 

Vertical 

direction 

Be careful not to touch the polarizer rotation ring and change the orientation during 

observation. If this happens. the orientation must be reset. 

27 
28 

m Focusing and centering the Bertrand lens 

When changing the objectives. turn the Bertrand lens 

focus ring under the Bertrand lens turret of the 

intermediate tube and focus the Bertrand lens. 

Centering the Bertrand lens is performed with two 

centering screws. The centering procedure is the same 

as that for the condenser except that the condenser 

aperture diaphragm image is used instead of the field 

diaphragm image. 

IE P-CL 1/4A. & tint plate 

Bertrand lens 

centering screws 

~, 

The P-CL l/4A & tint plate has a hole in the center. By pushing it into the slot. the sensitive tint 

plate (530 nm) is brought into the optical path. Pulling it out brings the l/4A plate into the optical 

path. This plate is used for recognition of very weak birefringence and the determination of X 

and Y' of the specimen. 

Ii] Centering the objectives 

Before centering the objectives, focus on a specimen using the !Ox objective. 

I Tools Used: Centering tools x 2 (provided) (when not in use, place them in the tool holder in 

the back of the microscope stand). 

1 Bring an appropriate target such as granules that can be easily used as a marker in the 

specimen to the center of the crosshairs of the eyepiece. 

2 Insert the centering tools into the centering screws on the nosepiece. 

3 Rotate the stage about 180°. Move the objective using the centering tools so that the center 

of the crosshairs moves by one-half the amount of movement of the target. 

4 Next. move the specimen and bring the target to the center of the crosshairs. 

5 Repeat this procedure several times. Carry out this centering procedure for each objective. 

180° rotation 

Target 

Middle point of 

the movement 

The centering nosepiece has a DIN standard slot. This allows the use of a 1141.. & tint plate 

compatible with DIN standards. 
3. Operation Of Each Part I 

llJ Circular graduated stage for polarizing microscope 

• Stage rotation 

Loosening the stage rotation clamp screw can rotate the 

stage. The angle of rotation can be read to 0.1 degrees 

from the two vernier scales. 

• 45° click-stop 

Turning the 45° click-stop lever towards the front until it 

stops causes the click-stop to move in 45° increments 

from the position where the lever was rotated. This 

makes it possible to move easily and accurately from the 

extinction position to the diagonal position. Turning the 

lever towards the back until it stops releases the clickstop device. 

Stage rotation 

clamp screw 

Release the click-stop device where the stage has dropped into a click position. If the clickstop device is released where the stage is not at this position. the first 45° increment will 

not be accurate the next time the click-stop device is operated. 

m Use of optional accessories (Sold Separately) 

P-CS Senarmont Compensator 

The P-CS Senarmont compensator is used by inserting 

into the slot of the intermediate tube in place of the 

P-CL 1141. & tint plate. 

It can be used to measure retardation up to I A according 

to the following procedure. 

It is normal to bring the top lens of the condenser into the optical path when observing at a 

magnification of !Ox or greater. Measuring the retardation or evaluating by interference 

color. however. stop down the condenser aperture diaphragm or swing out the top lens even 

for a magnification of !Ox or higher (with the aperture diaphragm fully opened). and 

illuminate with light that is as parallel to the optical axis as possible. 

29 
30 

1

1 Determination of Extinction Position 

Rotate the stage with the specimen under the crossed Nicols to find the direction where the 

part of the specimen to be measured appears darkest. 

r 

Determination of Subtraction Position 

Rotate the stage 45° from the extinction position to the diagonal position. Insert the P-CL 

l/4A. & tint plate into the optical path and confirm that the interference color of the section 

of the specimen to be measured changes toward the lower order side. If the interference 

color changes toward the higher order side. rotate the stage another 90°. 

3 Measurement 

Place the GIF filter on the field lens and replace the P-CL l/4A. & tint plate with the P-CS 

Senarmont compensator. Rotate the analyzer so that the section of the specimen to be 

measured becomes darkest. When the rotation angle of the analyzer at that time is taken to 

be theta (8) degrees. then retardation (R) (nm) is determined with the following formula: 

e R = 

180 

/... (/...: wavelength used) 

The value of A. when using the GIF filter is 546 nm. 

• 2. P-CQ Quartz wedge 

The P-CQ quartz wedge is used by inserting it into the 

slot of the intermediate tube in place of the P-CL l/4A. & 

tint plate. The quartz wedge is engraved with a scale 

and can be used for rough measurement of retardation in 

the range of I /...-6A.. 

It is normal to bring the top lens of the condenser into the optical path when observing at a 

magnification of IOX or greater. Measuring the retardation or evaluating by interference 

color. however. stop down the condenser aperture diaphragm or swing out the top lens even 

for a magnification of IOx or higher (with the aperture diaphragm fully opened). and 

illuminate with light that is as parallel to the optical axis as possible. 

Determination of Extinction Position 

Rotate the stage with the specimen under the crossed Nicols to find the direction where the 

part of the specimen to be measured appears darkest. 

2 Determination of Subtraction Position 

Rotate the stage 45° from the extinction position to the diagonal position (direction where 

the specimen appears brightest). Insert the P-CQ quartz wedge into the slot of the 

intermediate tube and confirm that the interference color of the section of the specimen to be 

measured changes toward the lower order side. If the interference color changes toward the 

higher order side. rotate the stage another 90°. 
3 Measurement 

Move the section of the specimen to be measured 

to the center of the crosshairs of the eyepiece. 

Next. slide the P-CQ quartz wedge along the slot 

and observe that the interference color 

sequentially changes. Stop sliding the quartz 

wedge where the dark stripe covers the section of 

the specimen to be measured. Reading the value 

from the quartz wedge scale at that time can make 

a rough measurement of retardation. 

Retardation can be measured even more 

accurately by using the P-CS Senarmont 

compensator in combination with the P-CQ quartz 

wedge. 

• 3. Attachable Mechanical Stage 

The attachable mechanical stage is installed by inserting 

the two pins on the bottom into the two pinholes on the 

stage surface. Tighten the clamp screw using the 

supplied hexagonal wrench. 

3. Operation of Each Part I 

Read the scale 

- Clamp screw 

31 
32 

Assembly 

WARNING To prevent electrical shock or fire. turn off the power switch (flip it to the •·C'" 

side) during assembly. 

CAUTION • Be careful not to pinch your hands or fingers when setting up th.: microscope. 

---- • Viewing will be adversely affected if any of the lenses is scratched or has 

fingerprints on it. Handle the lenses carefully during assembly. 

• This microscope is a precision optical instrument. Handle it cardully and do 

t · not subject it to a strong physical shock. (The accuracy of the objectives in 

particular may be adversely affected by even a weak physical shock. l 

Assemble each part in sequence as numbered in the diagram. (For details. refer tn pages 33 to 40.) 

I Tools needed 

• A hexagonal screwdrivers (provided) with the microscope 

• A hexagonal wrench (provided with the microscope) rWhen not using. place these in the tool holder in the back of the microscope. 

Installation location 

1.3 mm 

3mm 

This product is a precision optical instrument and using or storing it under unsuitable 

conditions may damage it or may have an adverse effect on its accuracy. The following 

conditions should be kept in mind when selecting the installation location. 

• Avoid installing the microscope in a brightly lit location such as a room that receives direct 

sunlight, or directly under room lights. The quality of the view through the microscope 

deteriorates if there is excessive ambient light. 

• Install the microscope in a location that is free from dust or dirt. 

• Install the microscope on a flat surface with little vibration. 

• Install the microscope on a sturdy desk or table that is able to hear the weight of the 

instrument. 

• Do not install the microscope in a warm. humid location (environmental temperature: 40°C or 

higher and relative humidity: 60lff or more). 
Photomicrographic vertical 

tube adapter 

-Clamp the photom1crograph1c 

vertical tuber adapter 

(3 screws) 

Eyepiece 

(b........_~ Trinocular 

~~eyepiece tube 

Clamp the eyepiece tube I.I l 

Analyzer slider 

~ = .J Intermediate 

Hexagonal 

screwdriver 

4. Assembly I 

a!~a.~=====:::i<oi:a ·--.... -~ b---= 9 tube - Hexagonal wrench 

P-CS Senarmont 

compensator 

P-CQ Quartz wedge [ 

P-CL 1/4i.. & tint plate J 

I Centering 

revolving 

nosepiece 

I Clamp the intermediate 

j tube 

+~ 

c•mp•o..-o~ nosep1ece 

- Centenng tools 

Lamphouse cover 

m 

12VDC 100W 

halogen lamp 

~ 

1 c1ampthe lampho.se i 

+ Clamp the 

lamphouse cover 

L...-~~~--~..W.,..,...._..J--J1'", 

~ ~Objective 

Specimen E600 POL main unit 

clips 

Circular graduated ----f 

stage for polarizing r 

microscope 

(including substage) 

lilJ r~ C•mp <"rnb"'" 

~ 

~ Clamp the condenser 

(left side) 

Swing-out 

condenser 

Lamphouse 

~ 

Power cord 

33 
34 

• 1. Confirming the input voltage 

i::J. - ·-

ltllton MOOEL ECLIPSE E600 POL 

100-120V24A 

SOISOHZ 

430001 

Make sure that the input voltage indicated on the back panel of the microscope 

is the same as the voltage provided in your area. If the indicated voltage is 

different. do not use the microscope and notify your nearest Nikon 

representative immediately. If the microscope is used with the wrong input 

voltage. a short circuit. electrical shock or fire may result. causing damage to 

the microscope. 

• 2. Lamp and lamphouse (lamp replacement) 

CAUTION • To prevent electrical shock and damage to the microscope. always turn off the 

...._.._,. power switch (flip it to the "0" side) and unplug the power cord when 

connecting or disconnecting the lamphouse. 

• To prevent burns. allow the lamp and lamphouse to cool before replacing it 

for at least 30 minutes after using. 

• Use the Nikon C-LP HALOGEN 12V-IOOW model for the lamphouse. 

•Use a 12 V-100 W LONGLIFE halogen lamp (OSRAM HLX 64623 or 

PHILIPS 7724 ). 

• Do not touch the glass surface of the lamp with your bare hands. Doing so 

will cause fingerprints, grease. etc .. to burn onto the lamp surface, reducing. 

the illumination provided by the lamp. If you do get any fingerprints or dirt 

on the lamp. wipe it clean. 

• After replacing the lamp. make sure that the lamphouse cover is attached 

securely. Never use the lamphouse while its cover is off. 

Clamp lever 
4. Assembly I 

1 Check that the power switch is off (i.e .. that it is flipped to the OO::J .. side). 

2 If the lamphouse is mounted on the microscope. use a hexagonal screwdriver to loosen the 

lamphouse clamp screw. and then remove the lamphouse from the microscope (the screw is 

on the top of the lamphouse mount). 

3 Use a hexagonal screwdriver to loosen the clamp screw on the front of the Jamphouse and 

remove the lamphouse cover. 

4 While pressing the lamp clamp lever. push the lamp into the socket pin holes as far as it will 

go. (Do not touch the glass portion of the lamp with your bare hands.) 

5 Return the clamp lever to its original position. Make sure that the lamp is not tilted. 

6 Attach the cover securely and tighten the clamp screw. Make sure that the cm·er is fixed in 

place and will not come off even when shaken by hand. 

7 Connect the lamphouse to the microscope by inserting the lamphouse plug into the 

lamphouse socket. 

8 Tighten the lamphouse clamp screw to secure the lamphouse in place. 

• 3. Stage assembly 

Substage (including circular graduated stage) 

Use a hexagonal wrench to loosen the clamp screw on the right side of the substage. 

Fit the substage over the substage mount on the microscope and slide it down as far as it 

will go. Use a hexagonal wrench to tighten the substage clamp screw and secure the 

substage in place. 

1

2 Specimen clip 

Insert the specimen clips into the holes on the stage surface. 

Condenser 

Turn the condenser focus knob to lower the condenser holder as far as it will go. r Insert the condenser in the condenser holder. Tighten the clamp screw on the left side with 

the aperture scale on the condenser facing to the front. 

Turn the condenser focus knob to raise the condenser as far as it will go. 

4 Observing a relatively thick specimen 

1 Turn the coarse focus knob to lower the 

substage as far as it will go. 

2 Loosen the substage clamp screw with a 

hexagonal wrench and remove the substage. 

3 Remove the upper limiting screw with a 

hexagonal wrench. (Store the screw in a safe 

place to prevent its loss.) 

4 Re-install the substage in the original position. 

/,,,,Remove 

the upper 

limiting screw. 

35 
36 

r • 4. Revolving nosepiece assembly 

1 Revolving nosepiece 

Use a hexagonal screwdriver to loosen the revolving nosepiece clamp screw. Fit the 

revolving nosepiece into the revolving nosepiece mount on the microscope from the bottom 

and slide it toward the rear of the microscope as far as it will go. Tighten th.: clamp screw 

to secure the revolving nosepiece. 

Note on removing the revolving nosepiece 

First. remove all the objectives. Lower the stage completely. and hold the n:volving 

nosepiece in your hand so that it does not fall when you remove it. 

2 Objective 

Lower the stage completely. Screw the objective into the revolving nosepiece so that the 

magnification increases when the nosepiece is rotated in the clockwise direction when 

looking down on the nosepiece from above. 

When using the objective for episcopic observation 

When using the objective for episcopic observation (Mark: =10) with the Epi-illuminator. 

first screw in the objective attaching screw conversion adapter (M20.32 --+ ~2.5 and 25mm 

in thickness) to the revolving nosepiece and then screw in the objective to the adapter. 

Note on removing objectives 

If there is a specimen on the stage. remove it first. Lower the stage comple!ely. and hold 

each objective in both hands so that they do not fall when you remove them. 

• 5. Intermediate tube 

Completely loosen the intermediate tube clamp screw on the microscope arm. Fit the circular 

dovetail of the intermediate tube into the circular dovetail groove of the microscope arm by tilting 

it at an angle. When fitting. insert the positioning pin on the intermediate tube in the receiving 

groove on the arm. Lock it in position by tightening the clamp screw. 

Remove any looseness between the positioning pin and groove by pushing in the 

intermediate tube while rotating in the clockwise direction. 
4. Assembly I 

·• 6. Eyepiece tube assembly 

1 Eyepiece Tube 

Completely loosen the eyepiece tube clamp screw on the imermediate tube with the 

hexagonal screwdriveL Fit the circular dovetail of the eyepiece tube into the circular 

dovetail groove of the intermediate tube by tilting it at an angle. When filling. insert the 

positioning pin on the eyepiece tube in the receiving groove on the intem1ediate tube. Lock 

it in position by tightening the clamp screw. 

Remove any looseness between the positioning pin and groove by pushing in the eyepiece 

tube while rotating in the clockwise direction. 

2 Eyepieces 

Attach eyepieces of the same magnification. Attach the eyepiece containing crosshairs on 

the right side so as to be viewed with the right eye. The sleeve has a positioning pins. 

Insert the eyepieces by aligning the grooves of the eyepiece with the positioning pins of the 

eyepiece tube sleeve. Insert the rubber eyegard (sold separately) so that they fit into the 

grooves around the outside of the eyepieces. 

• 7. Power cord ·;, . 

. 'Ii\ WARNING Use only the following power supply cord set. 

•For I00--120V area 

UL Listed. detachable cord set. 3 conductor grounding type SVT. No. 18 

AWG rated at 125V. 7A minimum. 

In case of using the extension cord. use only the power supply cord with the 

PE (protective earth) wire. 

• For 220--240V area 

3 pole power supply cord set. which must be approved according to EU/EN -

standards. 

Class I equipment should be connected to PE (protective earth) terminal. 

In case of using the extension cord. use only the power supply cord with the 

PE (protective earth) wire. 

Make sure to turn off the microscope power switch (flip it to the "0'' side) before connecting the 

power cord. 

Plug the cord into the socket of the AC input connector on the back of the microscope. Securely 

plug the other end of the cord into an AC outlet. 

Covering the tapped holes with protective stickers 

The four tapped holes on the upper surface of the microscope arm are for mounting accessories. 

such as Epi-fluon:scence attachment. etc. When not used, cover the tapped holes with the provided 

protective stickers. 

37 
38 

· · • Installing separately sold accessories 

Install photomicrographic equipment and other separately sold accessories by referring 10 the 

·i.c~~:,;;, instruction manual for each accessory. 

;.~ · • 8. Epi-illuminator assembly 

In the case of performing episcopic polarizing microscopy. the optional accessory epi-illuminator 

is used by mounting between the E600 POL stand and the intermedia1e tube. 

Assemble the epi-illuminator in the order of the numbers while referring to the diagram. Refer Ill 

the previous instructions for de1ails concerning lhe assembly procedures for 01her pans. 

Tnnocular 

eyepiece tube 

,-- Clamp the EpHllum1nator (4 bolts) 

I I I Filter Lamphouse cover + Duma slider sloe~ D Filter m 

lntermediatetube ~ I l - i + Clampthelamphouse ~12VDC100W 

O.mmy ""~:m"""""'~::;"" o 0 io i -· ~" """ 

Epi-illuminator 

E600POL 

Clamp the 

lamp house Ept-lamphouse 

1 Remove the eyepiece tube and 1he intermediale lube from the microscope s1and. 

2 Mount the epi-illuminator nearly parallel to the microscope arm. 

3 Tighten the epi-illuminator clamp screw on the right side of the arm with the hexagonal 

screwdriver to Jock in position. 

4 Screw in the four hexagonal head bolts supplied with the epi-illuminator to the screw holes 

on the top of the epi-illuminator. and tighten the bolts using 1he hexagonal wrench. 

5 Attach the lamp and lamphouse. 
···:; 

., ... -·x-·, 

,,,_.-.. ··· 

,.r·:.:i,; 

.,: ~ . 

~=: ;:;_-:; ~~= 

j.;;· ;;+= 

~-:;~ "'.-.- ... 

I 

4. Assembly I • To prevent electrical shock and damage to the microscope. always turn off the 

power switch (flip it to the "C" side) and unplug the power cord when 

connecting or disconnecting the lamphouse. 

• To prevent burns. allow the lamp and lamphouse to cool before replacing it 

for at least 30 minutes after using. 

• Use the Nikon LHS-HIOOP-2 HALOGEN 12V-100W model for the 

lamphouse. 

• Use a 12 V-100 W LONGLIFE halogen lamp (OSRAM HLX 64623 or 

PHILIPS 7724 ). 

• Do not touch the glass surface of the lamp with your bare hands. Doing so 

will cause fingerprints. grease. etc .. to burn onto the lamp surface. reducing 

the illumination provided by the lamp. If you do get any fingerprints or din 

on the lamp. wipe it clean. 

• After replacing the lamp. make sure that the lamphouse cover is attached 

securely. Never use the lamphouse while its cover is off. 

5·1 Check that the power switch is off (the 

switch is in the ··o·· position). 

5-2 If the lamphouse is already mounted on the 

epi-illuminator. loosen the lamphouse clamp 

screw with the hexagonal screwdriver and 

remove the lamphouse JJ. (The clamp 

screw is on the right side.) 

5-3 Loosen the lamphouse cover clamp screw 

with a coin or other tool llJ and remove the 

cover '31. 

5-4 Securely insert the lamp into the pinholes of 

the socket [[] until it does not go in any 

farther while pressing down on the lamp 

clamp lever ~-

Do not touch the glass portion of the lamp with 

your bare hands. 

5·5 Return the lamp clamp lever to its original 

position. 

Be careful so that the lamp is not installed tilting 

to one side. 

5-6 Securely close the lamphouse cover and 

tighten the clamp screw. 

5-7 After completely loosening the lamphouse 

clamp screw. securely plug the lamphouse 

plug into the socket of the lamphouse mount 

and then tighten the lamphouse clamp screw. 

39 
40 

4.anJ 

6 Connect the lamphouse cord to the epi-illumintor connector on the back of the microscope. 

7 Insert the hollow sliders all the way into the front and right sides of the epi-illuminaior so 

that the open hole enters the optical path. 

8 Attachment of Filter Slider (Various Filters) 

8-1 Remove the filter slider from the epi-illuminator. 

8-2 Pull out and remove the locking plate. 

8-3 Insert the desired filter. 

The filter slider contains two filters. 

8-4 Insert and attach the locking plate. 

8-5 Affix the label at the same position as the filter. 

8-6 Attach the filter slider to the epi-illuminator. 

9 Mount !he intermediate tube on the epi-illuminator. 

10 Mour .. 1he eyepiece tube on the intermediate tube. 

-8-5 

The eyepiece tube. intermediate tube and epi-illuminator are provided wi1h positioning 

pins. while the intermediate tube. epi-illuminaior and microscope arm are provided wirh 

grooves for engaging with those pins. Remove any looseness between the pins and grooves 

that occurs during assembly by pushing in on the component provided with the pin while 

turning in the clockwise direction. 
Troubleshooting Tables 

Improper use of the microscope may adversely affect the performance even if it is not damaged. If 

any of the problems listed in the table below occur. follow the countermeasures. 

D Viewing and control systems 

Problem Cause Countermeasure 

The optical path seleccion lever on the 

eyepiece cube is in an intermediace 

Sec the opcical pach seleccion leYer w position. I OO'K of the binocular eyepiece. 

The opcical path seleccion lever on the (p.201 

eyepiece tube is not sec IO I 00'7c of the 

binocular eyepiece. 

The diffuser is in an intermediace 

Insert and remove correccly. lp.2~) position. 

The revolving nosepiece has not been Install the revolving nosepiece 

installed properly. correctly. (p.36) 

The revolving nosepiece has not been Tum the revolving nosepiece until it 

rotated until ii has clicked into place. clicks imo place. (Place the objeccive in 

(The objeccive is not in the optical path.) the optical path. l (p.12) 

Vignetting or uneven Position the condenser so that the image 

brightness in the view field; The condenser is 100 low. of the field diaphragm forms properly on 

the entire view field cannot che specimen. (p.22) 

be seen. The condenser is not centered. Center the condenser. (p.22) 

The condenser is not installed properly. I nscall the condenser correct I y. (p.35) 

The filters are not switched fully into Switch !he filters correctly. (p.241 position. 

The field diaphragm is s!Opped down Open the diaphragm to a suitable size. 

(00 far. (p.24) 

The lamp is not installed properly. Install the lamp correctly. (p.34) 

The Bertrand lens is in the optical path. Move out of the optical path. 

The !Op lens of !he swing-ouc condenser Correctly push in all the way. is not positioned properly. 

The P-CL. P-CS or P-CQ plate is not Position correctly. inserted correct! y. 

Position the condenser so that the image 

The condenser is too low. of the field diaphragm forms properly 

on the specimen. (p.22) 

Dirt or dust in the view The aperture diaphragm is stopped down Open the diaphragm to a suitable size. field. 

too far. (p.23) 

There is dirt or dust on the lens. Clean the components. (p.45) condenser. eyepiece. filter or specimen. 

41 
Problem Cause Countermeasure 

The aperture diaphragm is stopped down Open the diaphragm to a suitable sin:. 

too far. lp.231 

Position the condenser so that the image 

The condenser is too low. of the field diaphragm forms proper!~ on 

the specimen. \p.22l 

The cover glass is too thick. Use the specified type of cover glass 

There is no cover glass. (thickness: 0.17 mm). 

There is no oil on the tip of an oilimmersion type of objective. 

Apply Nikon immersion oil. \p.2.'il 

The specified immersion oil is not being 

used. 

There are bubbles in th.:- immersion oil. Remo\'e the buhhl.:-~. lp.251 

Viewing is poor (too much There is immersion oil on the tip of a dry I 

or too little contrast, poor type of objective. Clean the components. lp.2.'i! 

resolution). The compensation ring on an objective 

fitted with a compensation ring has not Adjust the compensation ring according 

been adjusted. to the cover glass. 

There is dirt or dust on the lens. 

Clean the components. (p.4.'i) condenser. objective or specimen. 

A specimen with a cover glass is being Use an objective that allows observation observed with an objective for observing of specimens with a cover glass. specimens without a cover glass. 

A specimen without a cover glass is being Use an objective for observing specimens 

observed with an objective for observing without a cover glass. 

specimens covered with a cover glass. Mark on 

the objective 

oo/O.J7: For observing a specimen with 

a cover glass 

oo/O: For observing a specimen 

without a cover glass 

oo/-: For observing either specimen 

with or without a cover glass 

The revolving nosepiece has not been Install the revolving nosepiece correctly. 

installed properly. (p.36) 

Uneven focus. The revolving nosepiece has not been Tum the revolving nosepiece until it 

rotated until it clicks into place. clicks into place. lp.12) 

The specimen is not secured in place on Install the specimen properly in the 

the stage. specimen clips on the stage. 

The revolving nosepiece ha~ not been Install the revolving nosepiece correctly. 

installed properly. (p.36) 

The revolving nosepiece has not been Tum the revolving nosepiece until it 

Image flows. rotated until it clicks into place. clicks into place. (p.12) 

The specimen is not secured in place on Install the specimen properly in the 

the stage. specimen clips on the stage. 

The condenser is not centered. Center the condenser. lp.22) 

42 
5. Troubleshooting Tables I 

Problem Cause Countermeasure ! 

An NCB 11 filter is not being: used. Use the NCB 11 filter. (p.2~1 

The image is yellowish. 

The lamp voltage is too low. 

Push auto-photo switch and then adjust 

The image is too bright. The lamp voltage is too high. the brightness through the ND filter 

combination. l p.2~ and 25) The lamp voltage is too low. 

Inadequate illumination The aperture diaphragm is stopped down Open the diaphragm to a suitable size. 

(also check the electrical too far. \p.2>l 

system problems and 

Position the condenser so that the image countermeasures). The condenser is too low. of the field diaphragm fonns properly on 

the specimen. (p.221 

The specimen is placed upside-down. Set the specimen on the stage with the 

Focusing is not possible cover glass facing up. 

with high-power objectives. Use the specified type of cover glas~ The cover glass is too thick. uhickness: 0.17 mml 

The specimen is placed upside-down. Set the specimen on the stage with the 

The objective strikes the cover glass facing up. 

specimen when changing Use the specified type of cover glass from a low-power objective The cover glass is too thick. \thickness: 0.17 mml. 

to a high-power objective. 

The diopter setting has not been adjusted. Adjust the diopter setting. (p.21) 

The difference in focus is 

large when the objective is The diopter setting has not been adjusted. Adjust the diopter setting. (p.21) 

changed. 

When viewing through The interpupillary distance has not been Adjust the interpupillary distance. (p.21) 

the binocular eyepiece, adjusted. 

the image does not resolve 

into a single image. The diopter setting has not been adjusted. Adjust the diopter setting. (p.21) 

The interpupillary distance has not been Adjust the interpupillary distance. (p.21) 

Eye strain develops while adjusted. 

viewing. The diopter setting has not been adjusted. Adjust the diopter setting. (p.21) 

The brightness level is not suitable. Adjust the brightness through the ND 

filter combination. (p.24) 

43 
5. Troubleshooting Tables I 

El Electrical system 

Problem Cause Countermeasure 

The power is not being supplied. Plug the power cord into an outlet. \p.37) 

The power cord is not connected to the Connect the power cord. ip.:m The lamp does not light microscope. 

when the power switch is 

turned on. The lamp has not been installed. Install the lamp. \p.34l 

The lamp is burned out. Replace the lamp. !p.34) 

The specified lamp is not being used. Use the specified lamp trefer to the 

electrical specifications on p.46 l. lp .. ~4l 

The lamp is near the end of its life. Replace the lamp. lp.34l 

The power cord is not connected securely. Secure the connection. (p .. ~7l 

The lamp flickers; The lamp is not plugged into its socket Insert the lamp securely into its ~ocket. the brightness is unstable. securely. (p.34) 

The lamphouse is not connected to the I Connect the lamphouse securely. (p.34) microscope securely. 

44 
Care and Maintenance 

D Lens cleaning 

Do not let dust. fingerprints. etc. get on the lenses. Dirt on the lenses. filters. etc. will adversely 

affect the view of the image. If any of the lenses gets dirty. clean it as descrihed hdow. 

• Either brush away dust with a soft brush. or gently wipe away with gauze. 

• To remove fingerprints or grease. use a piece of soft. clean cotton cloth. lens tissue. or gauze 

moistened with absolute alcohol (ethyl alcohol or methyl alcohol). 

• Use petroleum benzine to clean off immersion oil (p. ::!5). 

• Do not wipe the entrance lens on the eyepiece tube with petroleum benzine. 

• Absolute alcohol and petroleum henzine are both highly flammahle. Be careful when 

handling them. when around open flames. or when turning the power switch on and off. 

• Follow the instructions provided by the manufacturer when using ahsolute alcohol and 

petroleum benzine. 

El Cleaning painted components 

Do not use organic solvents such as alcohol. ether or paint thinner on painted components. plastic 

components or printed components. Doing so could result in discoloration or in peeling of the 

printed characters. For persistent dirt. dampen a piece of gauze with neutral detergent and wipe 

lightly. 

IJ Storage 

Store the microscope in a dry place where mofd is not likely to form. 

Store the objectives and eyepieces in a desiccator or similar container with a drying agent. 

Put the vinyl cover over the microscope after use to protect it from dust. 

Before putting on the vinyl cover. turn off the microscope power (flip the switch to the" " 

position), and wait until the lamphouse has cooled. 

IJ Regular inspection 

Regular inspection of this microscope is recommended to maintain peak performance. Contact 

your nearest Nikon representative for details about n:gular inspection. 

45 
46 

Specifications and Standards 

- • 1. Model Name: 

• 2. Focusing Mechanism: 

• 3. Stage: 

• 4. Revolving Nosepiece: 

• 5. Illumination: 

~. • 6. Input Power Supply 

' Voltage: 

• 7. Protection Class: 

8. Operating Environmental 

Conditions: 

ECLIPSE E600 POL (microscope main body) 

• Fine focus knob scale: 

1 step equals I μm 

• Amount of movement of fine focus knob: 

One turn moves the stage up or down by 0.1 mm 

• Amount of movement of coarse focus knob: 

One turn moves the stage up or down by about 12 mm 

• Range of stage vertical motion: 

2 mm up and 23 mm down from the refrrence (focused) 

position 

• Circular graduated stage with two verniers 

• Provided with a rotation mechanism with a clamp 

• Provided with a 45° click-stop 

• Provided with two specimen clips 

•Quintuple 

• Provided with an objective centering mechanism 

• Internal Koehler-type diascopic illumination optics 

• Provided with a PHOTO switch (5-level fine adjustment 

selector) 

• Lamp rating: I 2V DC. I OOW halogen lamp 

(OSRAM HLX64623 or PHILIPS 7724) 

• AC I 00- l 20V ± I Olk 50/60Hz 

Current consumption: 

Internal fuse rating: 

• AC 230V ± 10% 50/60Hz 

Current consumption: 

Internal fuse rating: 

Class I 

o to +40°C 

2.4A or less 

125V, 5A 

l.2A or less 

250V. T3.15A 

• Temperature: 

•Humidity: 

• Altitude: 

85% RH max., non-condensing 

2000m max. 

• Pollution: Degree 2 

• Installation: Category II 

• Indoor use only 
Conforming Standards: 

• Product with AC 100-120V 

• Product with AC 230V 

7. Specttications and Standards ' '~.;: 

• UL Listed product 

• FCC !SB Class A satisfied 

This equipment has been tested and found to comply with the 

limits for a Class A digital device pursuant to Part IS of the FCC 

Rules. These limits are designed to provide reasonabk 

protections against harmful interference when the equipment is 

operated in a commercial environment. This equipment 

generates. uses. and can radiate radio frequency energy and if 

not installed and used in accordance with the instruction manual. 

may cause harmful interference to radio communications. 

Operation of this equipment in a residential area is likd: to 

cause harmful interference in which the user will be required tt> 

correct the interference at this own expense. 

This Class A digital apparatus meets all requirements of the 

Canadian Interference-Causing Equipment Regulations. 

Cet appareil numt!rique de la Classe A respecte toutes Jes 

exigences du Reglement sur le materiel brouilleur du Canada. 

• GS approved product 

• EU Low Voltage Directive satisfied 

• EU EMC Directive satisfied 

47 
ISO 9001 Certified 

1509001 --· 

MKON CORPORATION 

--Yokohama Division Plant 

NIKON CORPORATION 

9-16. Ohi 3-chame. Sh•nagawa-ku. Tokyo 140-8505. Japan 

Tel: +81-3-3773-812118122 Fax: +81-3-3773-8115 

NIKON INC. 

Instrument Group 

1300 Walt Whitman Roac:. Melville. NY 11747-3064. U.S.A. 

Tel: +1-516-547-8500 Fax: +1-516-547-0306 

NIKON EUROPE B.V. 

SchiphOlweg 321. P.O. Box 222. 1170 AE Badhoevedorp, The Netherlands 

Tel: +31-20-44-96-222 Fax: +31-20-44-96-298 

This instruction manual •S prin!ed on lhe recycled paper. M2 I 5 E 98.8.VFI