FreeZone® 6 Liter Benchtop

Freeze Dry Systems

Models 

7752020 

7752021 

7752030 

7752031 

7752040 

7752041 

 

User’s Manual

Labconco Corporation 

8811 Prospect Avenue 

Kansas City, MO 64132-2696 

800-821-5525, 816-333-8811 

FAX 816-363-0130 

E-MAIL labconco@labconco.com

HOME PAGE www.labconco.com

Please read the User’s Manual before operating the equipment. 

To receive important product updates, 

complete your product registration card 

online at register.labconco.com 
Copyright © 2004, 2007 Labconco Corporation. All rights reserved. 

The information contained in this manual and the accompanying products are copyrighted and all rights 

reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design 

changes without obligation to notify any person or entity of such change. 

Returned or Damaged Goods 

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be 

accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. 

Labconco Corporation and its dealers are not responsible for shipping damages. 

The United States Interstate Commerce Commission rules require that claims be filed with the delivery 

carrier within fifteen (15) days of delivery. 

Limitation of Liability 

The disposal and/or emission of substances used in connection with this equipment may be governed by 

various federal, state, or local regulations. All users of this equipment are required to become familiar with 

any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, 

land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to 

user’s compliance with such regulations. 

Contacting Labconco Corporation 

If you have questions that are not addressed in this manual, or if you need technical assistance, contact 

Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 

or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time. 

Part #7400209, Rev. C 

ECO E233 

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas 

of defective material and workmanship, provided such defect results from normal and proper use of 

the equipment. 

The warranty for all Labconco products will expire one year from date of installation or two years 

from date of shipment from Labconco, whichever is sooner, except the following; 

• Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Lab Animal Research 

Stations carry a three-year warranty from date of installation or four years from date of 

shipment from Labconco, whichever is sooner. 

• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from 

date of installation or three years from date of shipment from Labconco, whichever is 

sooner. 

• Blood Drawing Chairs carry a ten year warranty. 

• Carts carry a lifetime warranty. 

• Glassware is not warranted from breakage when dropped or mishandled. 

This limited warranty covers parts and labor, but not transportation and insurance charges. In the 

event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If 

the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair 

or replace all defective parts to restore the unit to operation. Under no circumstances shall 

Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This 

statement may be altered by a specific published amendment. No individual has authorization to 

alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by 

this warranty. Damage due to corrosion or accidental breakage is not covered.
TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION 1 

 Freeze Dry Process 1 

 Freeze Dry Rates 2 

 Freeze Dry Capacity 3 

 Samples Containing Volatile Substances 4 

About This Manual 4 

 Typographical Conventions 6 

 

CHAPTER 2: PREREQUISITES 7 

 Electrical Requirements 7 

 Location Requirements 8 

 Vacuum Pump Requirements 8 

 Chamber or Manifold Requirements 8 

 

CHAPTER 3: GETTING STARTED 9 

 Unpacking Your Freeze Dryer 9 

 Freeze Dryer Components 10 

 Setting Up Your Freeze Dryer 10 

 Vacuum Pump Connection 11 

 Electrical Connection 11 

 Drying Chamber or Drying Manifold Installation 11 

 Chemical Resistance of Freeze Dryer Components 11 

 Solvent Safety Precautions 13 

CHAPTER 4: USING YOUR FREEZE DRYER 15 

 Freeze Dryer Controls 16 

 Operation Checklist 17 

 Vacuum Pump Ballast Setting 18 

 Operating the Freeze Dryer 18 

 Set-Up 18 

 Automatic Start-Up 20 

 Manual Start-Up 21 

Setting the Operating Vacuum 21 

Pre-Freezing Samples 22 

Adding Sample 22 

Shut Down 24 
Defrosting 24 

Alarms 25 

Power Failure 25 

Line Voltage Out of Range 26 

Temperature Out of Range 26 

Service Vacuum Pump 26 

Moisture in Collector 27 

CHAPTER 5: MAINTAINING YOUR FREEZE DRYER 28 

CHAPTER 6: USING THE RS232 RECEPTACLE 30

 Computer Connection for Computer Interface 30 

CHAPTER 7: TROUBLESHOOTING 35 

 Vacuum Pump 35 

 Gaskets, Tubing, Connections, Sample Valves 36 

 System Components & Collection Chamber Isolation 38 

 Refrigeration Module Operation 41 

CHAPTER 8: MODIFYING YOUR FREEZE DRYER DISPLAY 42 

 Temperature and Vacuum Display 42 

 Line Voltage Alarm Points 42 

 Moisture Sensor Alarm 43 

 Modifying the Display 43

APPENDIX A: FREEZE DRYER COMPONENTS 47 

APPENDIX B: FREEZE DRYER DIMENSIONS 50 

APPENDIX C: FREEZE DRYER SPECIFICATIONS 51 

 Electrical Specifications 51 

 Environmental Conditions 52 

 Wiring Diagram (115V) 53 

 Wiring Diagram (230V) 54 

 

APPENDIX D: FREEZE DRYER ACCESSORIES 55 

DECLARATION OF CONFORMITY 64 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 1 

CHAPTER 1

INTRODUCTION

Congratulations on your purchase of a Labconco FreeZone® 

Freeze Dry System, which is designed for laboratory lyophilization 

procedures. The refrigerant used in the refrigeration system is 

CFC-free so it will not endanger the environment. The unit is easy 

to install and maintain. Proper care and maintenance of this 

product will result in many years of dependable service. 

Freeze Dry Process 

Freeze drying is an important process in sample preparation and 

for the preservation and storage of biologicals, pharmaceuticals 

and foods. Of the various methods of dehydration, freeze drying 

(lyophilization) is especially suited for substances that are heat 

sensitive. Other than food processing (e.g., coffee, whole dinners), 

freeze drying has been extensively used in the development of 

pharmaceuticals (e.g., antibiotics) and preservation of biologicals 

(e.g., proteins, plasma, viruses and cell lines). The nondestructive 

nature of this process has been demonstrated by the retention of 

viability in freeze dried viruses and microorganisms. 

Freeze drying is a process whereby water or other solvent is 

removed from frozen material by converting the frozen water 

directly into vapor without the intermediate formation of liquid 

water. The basis for this sublimation process involves the 

absorption of heat by the frozen sample in order to vaporize the 

ice; the use of a vacuum pump to enhance the removal of water 

vapor from the surface of the sample; the transfer of water vapor to 

a collector; and the removal of heat by the collector in order to 

condense the water vapor. In essence, the freeze dry process is a 

balance between the heat absorbed by the sample to vaporize the 
2 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 1: Introduction 

ice and the heat removed from the collector to convert the water 

vapor into ice. 

Freeze Dry Rates 

The efficiency of the freeze drying process is dependent upon the 

surface area and the thickness of the sample, the collector 

temperature and vacuum obtained, the eutectic point and solute 

concentration of the sample. It is important to remember these 

factors when trying to obtain efficient utilization of your freeze dry 

system. A listing of selected materials and their approximate 

drying times are shown in Table 1 for your reference. 

SAFE TEMPERATURE AND DRYING TIMES 

FOR SELECTED MATERIALS 

Material 

10mm Thick 

Safe 

Temperature

°C 

Collector 

Temperature 

°C 

Hours 

(Approx.) 

Milk -5 -40 10 

Urea -7 -40 10 

Blood Plasma -10 to -25 -40 16 

Serum -25 -40 18 

Vaccinia -30 to -40 -50 22 

Influenza Vaccine -30 -50 24 

Human Tissue -30 to -40 -50 48 

Vegetable Tissue -50 -80 60 

*Total sample quantities are contingent on various freeze dryer capacities. 

Up to the point of overloading the system, the greater the surface 

area of the sample, the faster the rate of freeze drying. By contrast, 

for a given surface area, the thicker the sample the slower the rate 

of freeze drying. This is based on the fact that the heat of 

sublimation is usually absorbed on one side of the frozen sample 

and must travel through the frozen layer to vaporize water at the 

other surface. In addition, as the sample is freeze dried, the water 

vapor must travel through the layer of dried material. The thicker 

the sample, the greater the chance that the dried layer may collapse 

which would cause an additional decrease in the rate of freeze 

drying. 

The surface area and thickness of the sample can usually be 

ignored when each sample contains only a few milliliters. 

However, for larger volumes, the samples should be shell frozen to 

maximize the surface area and minimize the thickness of the 

Table 1 
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Chapter 1: Introduction 

sample. The volume of the freeze dry flask should be two to three 

times the volume of the sample. 

In order for lyophilization to occur, ice must be removed from the 

frozen sample via sublimation. This is accomplished by the 

collector and the vacuum pump. The collector, which should be at 

least 15 to 20°C colder than the eutectic temperature (melting 

temperature) of the sample, traps vapor as ice. Since the vapor 

pressure at the collector is lower than that of the sample, the flow 

of water vapor is from the sample to the collector. Since this vapor 

diffusion process occurs very slowly under normal atmospheric 

conditions, a good vacuum is essential to maintain an efficient rate. 

In many applications, the maintenance of a vacuum of 0.133 mBar 

or less is recommended. 

The rate of freeze drying is directly proportional to the vapor 

pressure and the vapor pressure is dependent upon both eutectic 

temperature and solute concentration of the sample. For example, 

a solution of sodium chloride and water would freeze dry at a 

slower rate than pure water. The eutectic temperature of a sodium 

chloride solution is about –21°C and at this temperature the vapor 

pressure is about 1/16 that of water at 0°C. Although the eutectic 

temperature is not dependent upon the concentration of sodium 

chloride, the vapor pressure of the water would decrease as the 

concentration of sodium chloride increased. This is due to the fact 

that as the solute concentration increases, less of the surface area of 

the frozen sample is occupied by water. In general, most solutions 

or biological samples will have a eutectic temperature of –10° to 

–25°C. However, if the sample contains a simple sugar such as 

glucose or if the sample is animal or plant tissue, the eutectic 

temperature may be as low as –30° to –50°C. 

Freeze Dry Capacity 

The volume of a sample that can be freeze dried at one time is 

related to factors discussed previously and the size and design of 

the freeze dry system. With any given instrument, the capacity is 

based on the surface area of the sample; the eutectic temperature 

and concentration of the sample; and the rate and amount of heat 

transferred to the frozen sample. Of these factors, the eutectic 

temperature is the most important factor in determining the amount 

of sample that can be freeze dried at one time, particularly when 

flasks are used. This is because as the eutectic temperature 

decreases, the vapor pressure decreases but the rate of heat 

absorption by the sample does not change. This tends to promote 

melting of the sample, which leads to a marked increase in vapor 
4 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 1: Introduction 

pressure and ultimately overloads the collector and vacuum pump. 

Samples that have eutectic temperatures of –20°C or lower should 

be placed on the freeze dry system one flask at a time so that the 

vacuum in the system may recover before adding another sample 

to the system. If the vacuum does not recover, the capacity of the 

freeze dry system has been exceeded and the sample should be 

removed. 

If there is a problem with a particular type of sample melting when 

placed on the freeze dry system, dilution of the sample with more 

water or providing some insulation around the flask to decrease the 

rate of heat absorption by the sample may help. If the eutectic 

temperature of the sample is –40 to –60°C, the freeze dry system 

selected for use must be equipped with cascade type refrigeration 

so that the collector temperature can be cooled to below –75°C, or 

a dry ice/solvent trap can be used between the collector and the 

vacuum pump. 

Samples Containing Volatile 

Substances 

In certain cases the solvent in a sample to be freeze dried may 

contain volatile components such as acetonitrile, methanol, acetic 

acid, formic acid or pyridine. In addition to these substances 

having an effect on the eutectic temperature, they may increase the 

vapor pressure at the surface of the sample. Also, compared to 

water, they will require the absorption of less heat for sublimation 

to occur. Hence, samples that contain volatile substances will have 

a greater tendency to melt, particularly when placed in flasks or 

exposed to room temperature. If a sample containing a volatile 

substance tends to melt when placed on a freeze dry system, 

dilution of the sample with more water will help keep the sample 

frozen. For example, a 0.2M solution of acetic acid is much easier 

to freeze dry than a 0.5M solution. 

About This Manual 

This manual is designed to help you learn how to install, use, and 

maintain your Freeze Dryer. Instructions for performing routine 

maintenance and making minor modifications to your Freeze Dryer 

are also included. 

Chapter 1: Introduction provides a brief overview of the Freeze 

Dryer, explains the organization of the manual, and defines the 

typographical conventions used in the manual. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 5 

Chapter 1: Introduction 

Chapter 2: Prerequisites explains what you need to do to prepare 

your site before you install your Freeze Dryer. Electrical 

requirements are discussed. 

Chapter 3: Getting Started contains the information you need to 

properly unpack, inspect and install your Freeze Dryer. 

Chapter 4: Using Your Freeze Dryer discusses the basic operation 

of your Freeze Dryer. Information on how to attach samples and 

run the Freeze Dryer is included. 

Chapter 5: Maintaining Your Freeze Dryer explains how to 

perform routine maintenance on your Freeze Dryer. 

Chapter 6: Using the RS232 Receptacle describes how to connect 

a computer for monitoring the operation. 

Chapter 7: Troubleshooting contains a table of problems you may 

encounter while using your Freeze Dryer, including the probable 

causes of the problems, and suggested corrective actions. 

Chapter 8: Modifying Your Freeze Dryer Display describes how 

to calibrate the vacuum display and restore factory settings. 

Appendix A: Freeze Dryer Components contains labeled diagrams 

of the components of the Freeze Dryer. 

Appendix B: Freeze Dryer Dimensions contains comprehensive 

diagrams showing the dimensions for the Freeze Dryer. 

Appendix C: Freeze Dryer Specifications contains product 

specifications. A wiring diagram for the Freeze Dryer is also 

included. 

Appendix D: Freeze Dryer Accessories lists the part numbers and 

descriptions of all of the accessories available for your Freeze 

Dryer. 
6 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 1: Introduction 

Typographical Conventions 

Recognizing the following typographical conventions will help 

you understand and use this manual: 

• Book, chapter, and section titles are shown in italic type (e.g., 

Chapter 3: Getting Started). 

• Steps required to perform a task are presented in a numbered 

format. 

• Comments located in the margins provide suggestions, 

reminders, and references. 

• Critical information is presented in boldface type in paragraphs 

that are preceded by the exclamation icon. Failure to comply 

with the information following an exclamation icon may result 

in injury to the user or permanent damage to your Freeze 

Dryer. 

• Important information is presented in capitalized type in 

paragraphs that are preceded by the pointer icon. It is 

imperative that the information contained in these paragraphs 

be thoroughly read and understood by the user. 

Refer to Chapter 7: Troubleshooting if you are experiencing 

problems with your Freeze Dryer. 

! 

)
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 7 

CHAPTER 2

PREREQUISITES

Before you install your Freeze Dryer, you need to prepare your site 

for installation. Carefully examine the location where you intend 

to install your Freeze Dryer. You must be certain that the area is 

level and of solid construction. An electrical source must be 

located near the installation site. 

Carefully read this chapter to learn: 

• the electrical supply requirements. 

• the vacuum pump requirements. 

Refer to Appendix C: Freeze Dryer Specifications for complete 

Freeze Dryer electrical and environmental conditions, 

specifications and requirements. 

Electrical Requirements 

The Freeze Dryer requires a dedicated electrical outlet. This outlet 

requires a 20 Amp circuit breaker or fuse for models rated at 115V 

(60 Hz). An outlet equipped with a 15 Amp circuit breaker or fuse 

is required for models rated at 230V (50/60 Hz). The power cord 

on 115V models is equipped with a 20 Amp NEMA 5-20P plug. 

The power cord on 230V models is equipped with a NEMA 6-20P 

plug. If this does not match with the available receptacle, remove 

this plug and replace it with an approved plug of the suitable style. 
8 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 2: Prerequisites 

Location Requirements 

The Freeze Dryer should be located in an area that provides an 

unobstructed flow of air around the cabinet. This air cools the 

refrigeration system. The refrigeration system draws air in through 

the rear and exhausts it through the sides. A minimum of 3" must 

be allowed between the rear and both sides of the Freeze Dryer and 

adjacent wall surfaces. Restriction of airflow during operation 

could adversely affect performance. 

Refer to Appendix C: Freeze Dryer Specifications for dimensional 

drawings of the Freeze Dryer. The space for benchtop models 

must be large enough to accommodate the vacuum pump, which 

will be used with the Freeze Dryer. 

Vacuum Pump Requirements 

A vacuum pump must be provided by the user. A vacuum pump 

with a displacement of 144 liters per minute and 0.002 mBar 

ultimate pressure is adequate for most samples. The inlet fitting on 

the vacuum pump must be suitable for 3/4" ID vacuum hose, 

which is provided. It is recommended that the vacuum pump is 

equipped with an exhaust filter to minimize oil mist exhausting 

from the vacuum pump. The operating vacuum level may be set 

on the Freeze Dryer. The higher the pressure is set, the more likely 

it is that oil mist will be exhausted. 

Vacuum pumps used with 115V models should be equipped with 

an 115V, 15 Amp NEMA 5-15P plug. Vacuum pumps used with 

230V models should be equipped with a reverse IEC plug. This 

plug is included with 230V models. This will allow the vacuum 

pump to be plugged into the receptacle on the back panel of the 

freeze dryer. See Appendix D: Freeze Dryer Accessories for 

vacuum pumps available from Labconco. 

Chamber or Manifold 

Requirements 

A freeze drying chamber is not included and must be purchased 

separately. These allow samples to be attached to the Freeze 

Dryer. See Appendix D: Freeze Dryer Accessories for available 

chambers and manifolds. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 9 

CHAPTER 3

GETTING STARTED

Now that the site for your Freeze Dryer is properly prepared, you 

are ready to unpack, inspect, install and test your Freeze Dryer. 

Read this chapter to learn how to: 

• unpack and move your Freeze Dryer. 

• set up your Freeze Dryer. 

• connect the electrical supply source to your Freeze Dryer. 

• properly exhaust your Freeze Dryer. 

• safely use solvents with your Freeze Dryer. 

The FreeZone 6 Liter Benchtop Freeze Dry 

System weighs over 135 lbs. 61 kg). If you must 

lift the Freeze Dryer manually, use at least two 

(2) persons and follow safe lifting guidelines. 

Unpacking Your Freeze Dryer 

Carefully unpack your Freeze Dryer and inspect it for damage that 

may have occurred in transit. If your Freeze Dryer is damaged, 

notify the delivery carrier immediately and retain the entire 

shipment intact for inspection by the carrier. 

DO NOT RETURN GOODS WITHOUT THE 

PRIOR AUTHORIZATION OF LABCONCO. 

UNAUTHORIZED RETURNS WILL NOT BE 

ACCEPTED. 

!

The United States 

Interstate Commerce 

Commission rules 

require that claims be 

filed with the delivery 

carrier within fifteen (15) 

days of delivery. 

)
10 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 3: Getting Started 

IF YOUR FREEZE DRYER WAS DAMAGED IN 

TRANSIT, YOU MUST FILE A CLAIM 

DIRECTLY WITH THE FREIGHT CARRIER. 

LABCONCO CORPORATION AND ITS 

DEALERS ARE NOT RESPONSIBLE FOR 

SHIPPING DAMAGE. 

DO NOT DISCARD THE CARTON OR 

PACKING MATERIAL FOR YOUR FREEZE 

DRYER UNTIL YOU HAVE CHECKED ALL OF 

THE COMPONENTS AND INSTALLED AND 

TESTED THE FREEZE DRYER. 

Freeze Dryer Components 

Locate the model of Freeze Dryer you received in the following 

table. Verify that the components listed are present and 

undamaged. 

Catalog # Product Description 

7752020 6 Liter Benchtop Freeze Dryer – 115V, 60 Hz 

7752021 6 Liter Benchtop Freeze Dryer with Teflon-Coated Chamber – 115V, 60 Hz 

7752030 6 Liter Benchtop Freeze Dryer – 220/240V, 50 Hz 

7752031 6 Liter Benchtop Freeze Dryer with Teflon-Coated Chamber – 220/240V, 50 Hz 

7752040 6 Liter Benchtop Freeze Dryer – 208/230V, 60 Hz 

7752041 6 Liter Benchtop Freeze Dryer with Teflon-Coated Chamber – 208/230V, 60 Hz 

 

Plus the Following: 

Part # Component Description 

7400209 User’s Manual 

1336400 

1342100 

Power Cord – 115V 

 or 

Power Cord – 230V 

1291000 Plug (230V models only) 

7646000 Vacuum Hose 

1967600 Hose Clamps (2) 

7532900 Chamber Lid 

7690800 Lid Gasket 

If you did not receive one or more of the components listed for 

your Freeze Dryer, or if any of the components are damaged, 

contact Labconco Corporation immediately for further instructions. 

Setting Up Your Freeze Dryer 

After you verify receipt of the proper components, move your 

Freeze Dryer to the location where you want to install it. Then, 

follow the steps listed below. 

)

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 Product Service: Domestic 1-800-522-7658, International 816-333-8811 11 

Chapter 3: Getting Started 

Vacuum Pump Connection 

A vacuum pump as described in Chapter 2: Prerequisites is 

required to operate your Freeze Dry System properly. The Freeze 

Dryer is equipped with a 3/4" ID, heavy wall, vacuum hose for 

connecting the collector chamber to the vacuum pump. 

Make certain that the oil in the vacuum pump is 

at the proper level. 

Place the vacuum pump near the Benchtop Freeze Dryer. 

Connect the vacuum pump power cord to the receptacle on the 

back of the cabinet labeled “vacuum pump.” If the vacuum pump 

has an off/on switch, turn the switch on. The vacuum pump will 

be controlled by the Freeze Dryer. Connect the vacuum pump inlet 

port to the vacuum tube on the back of the cabinet with the vacuum 

hose and clamps provided. If necessary, cut the hose to proper 

length to allow for gentle bends without kinks. 

Models rated for use on 230V have a reverse IEC connector for 

attaching the vacuum pump power cord. A plug is supplied that 

can be installed on the vacuum pump power cord if necessary. 

Electrical Connection 

Plug the power cord into the receptacle on the back of the Freeze 

Dryer and plug the other end into a suitable power receptacle. 

Drying Chamber or Drying Manifold 

Installation 

The drying chamber or drying manifold, purchased separately, may 

be positioned directly above the 3-inch port on the top of the 

Freeze Dryer. Follow the instructions supplied with the selected 

accessory. 

Chemical Resistance of Freeze Dryer 

Components 

The FreeZone Freeze Dry System is designed to be chemically 

resistant to most compounds that are commonly used in freeze 

drying processes. However, by necessity, the freeze dryer is 

comprised of a number of different materials, some of which may 

!
12 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 3: Getting Started 

be attacked and degraded by certain chemicals. The degree of 

degradation is dependent on the concentration and exposure 

duration. Some of the major components of the FreeZone Freeze 

Dry System that are susceptible to degradation are as follows: 

 Acids Buffers Solvents 

Component Material 

Acetic Acid 20% Formic Acid Trifluoroacetic Acid (TFA) Calcium Chloride Sodium Phosphate Acetone Acetonittirle Carbon Tetrochloride Cyclohexane Dioxane Methyl t-Butyl Ether (BTBE) Pyridine 

Valve Stem Acetal 

(Delrin) 

C D D D D 

Collector* 

Lid 

Acrylic D D D D 

Hoses, 

Gaskets & 

Valve 

Bodies 

Neoprene C D D C C D D D C D 

Flask Top Silicon 

Rubber 

 C D D D D D C D 

Chamber & 

Fittings 

Stainless 

Steel 

 C 

* An accessory glass lid is available. 

C – Moderate degradation; Limited use. 

D – Severe degradation; infrequent use recommended; immediate 

 thorough cleaning required. 

• Most common compounds used in freeze drying processes, if 

allowed to enter the vacuum pump, will degrade the oil and 

cause damage to the vacuum pump. 

• Sugars and proteins typically will have minimal negative effect 

on any of the materials of construction. 

When using compounds in the Freeze Dryer that are hostile to the 

materials of construction, it is imperative the equipment is 

thoroughly cleaned after use. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 13 

Chapter 3: Getting Started 

• Rubber and plastic components that have been exposed to 

damaging compounds should be removed and flushed with 

water. 

• The oil in the vacuum pump should be checked often. It must 

be changed if it is cloudy, shows particles or is discolored. The 

useful life of vacuum pump oil can be extended if the vacuum 

pump is operated for an extended period of time after a freeze 

dry run. This allows contaminants to be purged from the hot 

oil. This must be done with the inlet to the pump blocked off 

to prevent air from free flowing through the pump. This is 

accomplished by closing all sample valves on a clean, dry 

freeze dry system and turning on the vacuum pump. If the 

pump is operated at an elevated vacuum level (> 10mBar), oil 

may be expelled from the pump and damage could occur. 

Another way to extend the life of the vacuum pump is to install an 

optional secondary trap in the line between the Freeze Dryer and 

the vacuum pump. Contact Labconco for ordering information. 

With prudent maintenance the FreeZone Freeze Dry System will 

provide years of service. Warranty on the affected parts will be 

voided if maintenance has been obviously neglected. If you have 

questions about using specific compounds in the Freeze Dryer, 

contact Labconco Technical Service at 1-800-821-5525 or 816-

333-8811 or e-mail: labconco@labconco.com. 

Solvent Safety 

Precautions 

Solvents used in the Freeze Dryer may be 

flammable or hazardous to your health. Use 

extreme caution and keep sources of ignition 

away from the solvents. When using flammable 

or hazardous solvents, the vacuum pump must 

be vented to or operated inside a fume hood. 

Hazardous materials such as strong acids or 

bases, radioactive substances and volatile 

organics must be handled carefully and 

promptly cleaned up if spilled. If a sample is 

spilled in the collector chamber it must 

immediately be cleaned up. 

!
14 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 3: Getting Started 

WARNING: The disposal of substances used in 

connection with this equipment may be governed 

by various Federal, State or local regulations. 

All users of this equipment are urged to become 

familiar with any regulations that apply in the 

user’s area concerning the dumping of waste 

materials in or upon water, land or air and to 

comply with such regulations. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 15 

CHAPTER 4

USING YOUR FREEZE

DRYER

After your Freeze Dryer has been installed as detailed in Chapter 

3: Getting Started, you are ready to begin using your Freeze Dryer. 

Read this chapter to learn how to: 

• operate the controls. 

• understand the display. 

• connect samples. 

Do not use the Freeze Dryer in a manner not 

specified by the manufacturer (refer to Appendix 

C: Freeze Dryer Specifications). The electrical 

protection properties of the Freeze Dryer may be 

impaired if the Freeze Dryer is used 

inappropriately. 

!
16 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

Freeze Dryer Controls 

The control panel for the Freeze Dryer is shown below with a 

description about its function. 

1. LCD Display – Displays system operating parameters, set-up 

parameters and alarm messages. 

2. Menu Switch – This switch is used to change the display from 

operating system parameters to set-up parameters. 

3. Select Switch – Used to select set-up parameters. 

4. Vacuum Switch – Used to start or stop the vacuum pump when 

operating in manual start-up mode. 

5. Vacuum Indicator – This green LED indicates that power is being 

supplied to the vacuum pump receptacle on the back of the Freeze 

Dryer. 

6. Manual Refrigeration Switch – Used to start only the refrigeration 

module. 

7. Manual Operation Indicator – When lit, the green LED indicates the 

Freeze Dryer is being controlled manually by the operator. The 

operator must start each function. 

8. Auto Mode Switch – Used to start or stop the refrigeration and the Auto 

Mode process. 

1 

4 

7

9 

12 

8 

6 

3 

11 

5 2 10 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 17 

Chapter 4: Using Your Freeze Dryer 

9. Auto Mode Indicator – When lit, the green LED indicates that the 

Freeze Dryer is in Auto Mode. In this mode, the vacuum pump will 

start when the collector temperature reaches –40°C. 

10. Vacuum Graph Display – This display indicates the relative system 

vacuum level. The highest LED indicates that the vacuum level is 

above 2.0 mBar. The indicators will sequence down when the vacuum 

level reaches 2.0, 1.0, 0.8, 0.6, 0.45, 0.12 mBar. The lower green LED 

flashes when the system vacuum level is 0.45 to 0.12 mBar and 

illuminates steadily below 0.133 mBar. 

11. Collector Temperature Graph Display – This display indicates the 

temperature of the collector. The highest LED indicates the collector 

temperature is warmer than 10°C. The indicators will sequence down 

when the temperature reaches 10, 0, -10, -20, -30, -40°C. When the 

collector temperature is –40°C or lower the green indicator will light. 

12. Alarm Indicator – This red LED indicates that a system alarm has 

occurred. Press the Menu Switch to display the alarm message on the 

LCD display. 

13. Main Power Switch – Turns the Freeze Dryer on or off. (Not shown, 

located on the left side of the cabinet.) 

Operation Checklist 

The following checklist should be followed prior to each use of 

your Freeze Dryer: 

1. Wipe the interior of the collector chamber with a soft cloth or 

paper towel to remove any accumulated moisture. 

2. Check the collector chamber drain hose to ensure that the hose 

is free of moisture and that the drain plug is securely installed. 

The Freeze Dryer will not start if moisture is detected. 

3. Using a soft, lint-free cloth or paper towel, wipe the collector 

chamber lid gasket to remove any dirt and contaminants that 

could cause a vacuum leak. Vacuum grease is not required on 

the lid gasket to obtain a proper vacuum seal. 

4. Remove the accessory drying chamber or manifold from the 

connection port and using a soft, lint-free cloth or paper towel, 

wipe the port gasket and sealing surfaces of the drying 

chamber/manifold to remove any dirt and contaminants that 

could cause a vacuum leak. Reinstall the drying chamber or 

manifold on the port. Vacuum grease is not required on the 

port gasket to obtain a proper vacuum seal. 
18 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

5. Inspect each sample valve on the accessory drying chamber or 

manifold and check for any visible damage and for improper 

installation that might cause a vacuum leak. Also check that 

each sample valve is closed or in the “vent” position. 

Vacuum Pump Ballast Setting 

Most vacuum pumps are equipped with a gas ballast mechanism. 

The freeze dry process requires high vacuum. Therefore, it is 

recommended that the gas ballast be closed during the operation of 

the Freeze Dry System. If the gas ballast is left open for extended 

periods of operation, the oil can be pumped out the exhaust, 

causing the pump to fail. 

Operating the Freeze Dryer 

Set-Up 

The Freeze Dryer may be configured to automatically start the 

vacuum pump when the collector temperature reaches –40°C. The 

display units for vacuum may be selected to be mBar, Pascal (Pa) 

or Torr and the temperature may be displayed as °F or °C. The run 

time of the refrigeration system and the vacuum pump may be 

monitored. 

To configure your Freeze Dryer, turn the main power switch ON 

and press MENU. The display will show: 

• Press SELECT until the desired units are flashing. 

• Press MENU. 

• The display will show: 

Where YYY is the units selected above. 

VACUUM UNITS 

mBar Pa Torr 

VACUUM SET POINT 

XXXX YYY 
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Chapter 4: Using Your Freeze Dryer 

• Press SELECT until the desired vacuum operating level is 

displayed. See “Setting the Operating Vacuum Level” for 

guidelines 

• If “O” is selected, the vacuum control is disabled and the 

vacuum level in the Freeze Dry System will be determined 

by the capabilities of the vacuum pump. 

• Press MENU. 

• The display will show: 

• Press SELECT until the desired units are flashing. 

• Press MENU. 

• The display will show: 

• To reset the SERVICE HOUR to 0 press SELECT. This 

allows you to keep track of the time the refrigeration 

system operated since it was serviced. 

• The display will show the refrigeration system hours. 

• To reset the service hours to 0, continue to hold SELECT 5 

seconds until hours are reset. 

• TOTAL HOUR cannot be reset. 

• Press SELECT. 

• The display will show the vacuum pump hours. 

TEMPERATURE UNITS 

°C °F 

REFRIG TOTAL HOUR: XXXX 

 SERVICE HOUR: XXXX 

REFRIG TOTAL HOUR: XXXX 

RESET SERVICE HOUR: ? 

VACUUM TOTAL HOUR: XXXX 

 SERVICE HOUR: XXXX 
20 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

• To reset the SERVICE HOUR to 0, press SELECT. 

• The display will show: 

If you want to reset the service hours to 0, continue to hold 

SELECT five seconds until hours are reset. 

• Press SELECT. 

• The display will show the RS-232 data transmission rate. 

• To change the rate press and hold SELECT until the 

desired time interval is shown. The time between data 

transmissions may be set to occur at 10, 30, 60, 300 or 600 

second intervals. 

• Press SELECT to return to display operating parameters or 

after a short delay, the display will automatically switch to 

show operating parameters. 

Automatic Start-Up 

To run the Auto Mode, press the panel switch labeled 

REFRIGERATION AUTO. The green LED above the switch will 

illuminate. This will start the refrigeration system. When the 

collector reaches –40°C, the vacuum pump will start. The 

Temperature and Vacuum Graphs will indicate collector 

temperature and system vacuum. The LCD display will show the 

actual temperature of the collector. When the vacuum in the 

system is above 5 mBar the vacuum display will indicate “HI.” At 

5 mBar and below, the display will show the actual vacuum. 

When the system vacuum is between 0.450 and 0.133 mBar, the 

lower green vacuum graph LED will flash. When the system 

vacuum level is <0.133 mBar, the green LED will be lit steadily 

indicating that most samples may be added. 

RS-232 TRANSMISSION RATE 

10 SECONDS 

VACUUM TOTAL HOUR: XXXX 

RESET SERVICE HOUR: ? 
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Chapter 4: Using Your Freeze Dryer 

Manual Start-Up 

To manually run the freeze dry process, press the 

REFRIGERATION MAN switch. This will start the refrigeration 

system. The green LED above the switch will illuminate. When 

the collector temperature reaches –40°C, the vacuum pump may be 

started by pressing the VACUUM switch. The Temperature and 

Vacuum Graphs will indicate collector temperature and system 

vacuum. The LCD display will show the actual temperature of the 

collector. When the vacuum in the system is above 5 mBar the 

vacuum display will indicate “HI.” At 5 mBar and below, the 

display will show the actual vacuum. 

When the system vacuum is between 0.450 and 0.133 mBar, the 

lower green vacuum graph LED will flash. When the system 

vacuum level is <0.133 mBar, the green LED will be lit steadily 

indicating that samples may be added. 

Setting the Operating Vacuum 

Level 

The vacuum level may be set by the user to optimize the freeze dry 

process. Normally, the sublimation rate will increase if there is 

less vacuum (a higher pressure) in the Freeze Dryer. A good 

starting place is to set the vacuum so its level is equivalent to about 

10°C colder than the eutectic or collapse temperature of the 

sample. Adjustments to the vacuum level must be made for 

various freeze drying conditions. Factors that must be considered 

are whether the sample is freeze dried on heated shelves or in 

glassware attached to manifold valves, the volatility of the sample 

itself, the size of the sample and the heat energy supplied to the 

sample. 

When the vacuum control is set to operate at less vacuum, the ice 

holding capacity of the collector may be decreased. This can be 

improved by installing a baffle in the collector. See Appendix D 

Freeze Dryer Accessories for the baffle part number. Position the 

baffle so it rests on the top of the collector coil. The arrow should 

point toward the front. 
22 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

Some guidelines for setting the Freeze Dryer are shown below. 

These show pre-freezing temperatures and vacuum levels. Exact 

protocols must be determined by the user for the specific samples 

that are being freeze dried. 

Material Solidification/Eutectic 

Temperature 

Pre-Freeze 

Temperatures Vacuum Set Point 

Bacteria,Virus -40°C and Colder -50°C & 

Colder 

0.040 mBar & 

Lower 

Milk -5 to -13 -15 to -23 1.65 to 0.77 

Fungi -40 and Colder -50 & Colder 0.04 and lower 

Vegetable Tissue -25 to -50 -35 to -60 0.22 to 0.01 

Human Tissue -30 to –40 -40 to –50 0.12 to 0.04 

Blood Plasma -10 to –25 -20 to –35 1.03 to 0.22 

Vaccine -30 to –40 -40 to –50 0.12 to 0.04 

Pre-Freezing Samples 

Appropriate containers for freeze drying include ampules, serum 

bottles, and wide mouth freeze drying flasks. Shell freezing of 

samples is recommended for wide mouth freeze drying flasks. 

Smaller samples in ampules and serum bottles may be frozen in a 

freezer. The sample container size should always be at least two to 

three times the sample size (i.e., 40 ml samples should be prepared 

in 80 ml containers or larger). The temperature required for prefreezing is dependent on the characteristics of the sample. Prefreezing temperature typically is at least 10° to 20°C below the 

eutectic or collapse temperature of the sample. 

Adding Sample 

The following procedure should be followed when using sample 

valves in the freeze dry process: 

1. Connect a pre-frozen sample to a sample valve on the drying chamber 

or manifold using an adapter. Turn the plastic valve knob to the 

“VACUUM” position to open the valve. The bevel on the knob 

should be positioned toward the sample port to apply vacuum to the 

sample. 
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Chapter 4: Using Your Freeze Dryer 

2. Before adding another sample, allow system vacuum to return 

to 0.133 mBar or lower. Any combination of valves and 

sample sizes may be utilized at one time provided that the 

system vacuum and collector temperature remain sufficiently 

low to prevent melting of the frozen sample. 

3. When all the frost has disappeared from the outer surface of the 

sample container and no cold spots can be detected by handling 

the container, the sample is nearly dry. To be certain of low 

final moisture content, dry the sample for several hours past 

this point. 

4. To remove a container after drying is complete, turn the plastic 

knob on the valve to the “VENT” position, which closes the 

valve and vents the container. Should backfilling with an inert 

gas be required, connect the gas supply line to the vent port on 

the valve before turning the plastic knob on the valve to vent 

position. The sample container may now be removed. In the 

vent position the bevel on the valve knob should point away 

from the sample port. 

5. Ampules may be flame sealed while connected to a valve by 

using a sealing torch. Care must be taken not to burn the valve. 

An insulation material placed between the valve and the torch 

is recommended. 

Vent/Backfill Port 

Bevel in “vent” position 

Bevel in “vacuum” 

position 

Adapter 
24 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

Shut Down 

At the end of a run or when a sufficient amount of condensate 

accumulates on the collector coil to obstruct the flow of vapor to 

the collector chamber, the Freeze Dryer should be defrosted. First, 

release system vacuum by turning the plastic knob on a valve to 

the open position or by pulling the collector chamber drain plug 

out of the drain hose. Now press the Vacuum Switch on the 

control panel to turn the vacuum pump OFF. Press the 

Refrigeration Switch next to the illuminated LED to turn OFF the 

refrigeration system. Turn OFF the Main Power Switch on the left 

hand side of the cabinet. 

Defrosting 

The following procedure should be followed when defrosting the 

collector coil: 

1. Pull the collector chamber drain hose out from the right hand 

side of the Freeze Dryer and remove the drain plug. Place the 

drain hose in a suitable container to collect the condensate that 

will melt off the collector coil. 

2. Remove the collector chamber lid and allow ambient room air 

to melt the ice. Dispose of the liquid appropriately. 

3. Flush the collector chamber with water and wipe chamber dry. 

4. If rapid defrost is desired, pour warm water over the collector 

coil. Do not allow the liquid to enter the vacuum port on the 

upper rear of the chamber. 

5. Reinstall the drain hose plug and slide drain hose back into the 

side of the cabinet. Dispose of the liquid appropriately. 

Utilization of acid requires immediate cleaning 

and neutralization after defrost or physical 

damage to the collector chamber and collector 

coil will result. 

Do not attempt to chip ice from the collector coil 

as damage may occur to the coil. 

Never attempt to start the vacuum pump when 

there is liquid in the collector chamber. This 

could result in damage to the vacuum pump. 

! 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 25 

Chapter 4: Using Your Freeze Dryer 

Alarms 

A number of unusual events may occur during a lyophilization 

procedure that can adversely effect the operation of the Freeze 

Dryer. If an event occurs, the alarm indicator will flash and the 

beeper will sound. 

The beeper will automatically mute itself after one minute. The 

specific alarm can be identified by pressing MENU. Pressing 

MENU multiple times will display multiple alarms if they have 

occurred. The following “out of specification” conditions will 

initiate an alarm: 

Power Failure 

If a power failure occurs during a Freeze Dry process, the vacuum 

control valve will allow air to bleed into the Freeze Dryer System. 

If the failure is of a short duration and the collector does not warm 

up excessively, when power is restored the Freeze Dryer will 

restart and resume operation of the refrigeration and vacuum 

systems. If the power failure lasts for a longer duration and the 

collector warms above safe limits, when the power is restored, the 

Freeze Dryer will not automatically restart. This prevents melted 

sample from being drawn into the collector and prevents liquid 

from being sucked into the vacuum pump. 

When power is restored, the alarm indicator will flash and the 

alarm beeper will sound. When the MENU button is pressed the 

display will show 

Press and hold SELECT for five seconds until the error message is 

cleared from the display. 

POWER FAIL 

HOLD SELECT TO CLEAR IT 
26 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 4: Using Your Freeze Dryer 

Line Voltage Out of Range 

If the voltage supplied to the Freeze Dryer varies beyond allowable 

limits, the alarm will be activated. When an alarm occurs, press 

MENU and the display will show: 

The alarm will self-cancel when the proper voltage is restored. 

The high and low alarm points are preset at the factory to 

correspond to the normal allowable voltage variations based on the 

nominal voltage specified for the freeze dryer. Some models may 

be operated outside the normal voltage limits. See Appendix C. 

If necessary, the high and low alarm points may be adjusted for 

these models. See Chapter 8. 

Temperature Out of Range 

An alarm will be activated if the collector temperature rises above 

–40°C. If this occurs, the display will show: 

Press and hold SELECT for five seconds until the error message is 

cleared from the display. 

Service Vacuum Pump 

The vacuum pump normally plugs into the vacuum pump electrical 

receptacle on the back of the Freeze Dryer. When the Freeze 

Dryer has accumulated a total of 1000 operating hours, the alarm 

indicator will flash. Press MENU to display the alarm: 

Press and hold SELECT for five seconds until the error message is 

cleared from the display. 

This will reset the 1000 hour timer. It may be necessary to service 

the vacuum pump more frequently than every 1000 hours 

depending on the operation of the Freeze Dryer. The pump oil 

should be regularly monitored to verify that it is clean. 

LINE VOLTAGE ERROR 

COLLECTOR TEMPERATURE 

HOLD SELECT TO CLEAR IT 

CHANGE VACUUM OIL 

HOLD SELECT TO CLEAR IT 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 27 

Chapter 4: Using Your Freeze Dryer 

Moisture in Collector 

If there is liquid in the collector chamber and either a 

REFRIGERATION button or the VACUUM button is pressed, 

neither function will start and an alarm will be activated. When 

MENU is pressed, the display will show: 

When the collector is drained, the alarm will self-cancel. 

This feature prevents the inadvertent start of the vacuum pump 

when liquid is in the collector and thereby can extend the life of 

the vacuum pump. A few drops of liquid may remain in the drain 

hose after draining. When the vacuum pump is started these drops 

may be sucked into the collector chamber. This is normal. 

MOISTURE IN COLLECTOR 
28 Product Service: Domestic 1-800-522-7658, 

CHAPTER 5

MAINTAINING YOUR

FREEZE DRYER

Under normal operation, the Freeze Dryer requires little 

maintenance. The following maintenance schedule is 

recommended: 

As needed:

1. Clean up all spills; remove liquids from the chamber. 

2. Clean lid and gasket using soft cloth, sponge or chamois and a 

mild, non-abrasive soap or detergent. 

3. Check oil level of the vacuum pump. It should be between 

MIN and MAX. If the oil level is less than an inch (25.4 mm) 

above MIN, add oil to proper level. 

4. If oil shows cloudiness, particles or discoloration, drain the 

pump and replace with fresh oil. 

5. Utilization of acids requires immediate cleaning and 

neutralization after a run or physical damage to the 

collector chamber and collector coil will result. 

6. Check the collection chamber for condensed or frozen solvents 

and dispose of appropriately. Completely empty the collector 

chamber before the next run or before attempting to start the 

vacuum pump. 

7. If sample valves leak or stems become difficult to turn, pull the 

stem out of the rubber valve body and apply a thin coat of 

vacuum grease to the stem. Reinsert the stem into the valve 

body. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 29 

Chapter 5: Maintaining Your RapidVap 

Monthly:

1. The rubber components on the Freeze Dryer may eventually 

deteriorate and require replacement. The effective life of 

rubber parts depends upon both their usage and the surrounding 

environment. Check all rubber hoses and gaskets and replace 

any that show signs of hardening, permanent set or 

deterioration. 

2. Using a soft cloth, sponge or chamois and a mild, non-abrasive 

soap or detergent, clean the acrylic chamber lid. 

3. Using a soft cloth, sponge, or chamois and a mild, non-abrasive 

soap or detergent, clean the exterior surfaces of the Freeze 

Dryer. Liquid spray cleaners and polishes may be used on the 

exterior surfaces. Do not use solvents to remove stains from 

the exterior surfaces as they may damage the finish. 

Annually:

1. Every 12 months, or more often if the Freeze Dryer is operated 

in a dusty environment, the refrigeration system condenser 

should be cleaned. Using a vacuum cleaner with brush 

attachment, clean the condenser to ensure proper airflow for 

peak performance. 
30 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

CHAPTER 6

USING THE RS232

RECEPTACLE

The operation of the Freeze Dryer can be monitored using a 

computer when it is connected to the RS232 receptacle on the rear 

panel. The computer cannot control the operation of the Freeze 

Dryer. The monitored parameters are the collector temperature in 

°C and vacuum in microbars. 

When a Labconco Stoppering Tray Dryer is connected to a 

Labconco Freeze Dryer as detailed in the Stoppering Tray Dryer 

User’s Manual, the status of the Stoppering Tray Dryer may be 

monitored simultaneously with the status of the Freeze Dryer. 

Computer Connection for 

Computer Interface 

Check your computer to see which type of serial port is provided, 

then use a connecting cable below: 

1. Computers with a 25 pin D-sub male serial connector 

should use Connect Cable, Labconco part number 7537801, 

to connect a computer to the Labconco Freeze Dryer. 

2. Computers with a 9 pin D-sub male serial connector should 

use Connect Cable, Labconco part number 7537800, to 

connect a computer to the Labconco Freeze Dryer. 

The purpose of the RS232 interface is to send data to a data 

logging computer to monitor the state and activity of the Freeze 

Dryer. This data is half duplex data. The data properties are as 

follows: 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 31 

Chapter 6: Using the RS232 Receptacle 

1. Data Rate 2400 Baud 

2. 8 Bit word length 

3. 1 Start bit, 1 Stop bit 

4. No parity is transmitted 

5. Standard ASCII character set 

The time between data transmissions may be varied by the user to 

occur at 10, 30, 60, 300 or 600 second intervals. Press the MENU 

button until the RS232 screen appears on the display. 

Press select until the desired time interval is shown. 

The format of the transmitted message is as follows: 

B<T= -48 V= 18 SF = 999 C=999 PG=9 > 

Where: 

 B = Base Unit 

 T = Collector Temperature 

-48 = Collector Temperature in °Celsius 

 V = Vacuum 

18 = Vacuum Level in Micro Bars 

SF = Shell Freezer Temperature (not available on 6L 

 Benchtop models) 

 9 = Feature not installed or out of range 

 C = Vacuum Chamber Temperature (not available on 

 6L Benchtop models) 

PG = Purge (not available on 6L Benchtop models) 

If a Stoppering Tray Dryer is attached to the Freeze Dryer and the 

data interconnect cable is installed, additional data about that 

accessory will be transmitted from the Freeze Dryer. See the 

User’s Manual for the Stoppering Tray Dryer for a description of 

the data. 

There are several commercially available software packages, 

which can read RS232 data and enter the data into a computer 

program such as a word processor (to create a text file) or 

spreadsheet (to tabulate and plot the data). Consult your laboratory 

supply dealer regarding the latest software available. The 

RS-232 TRANSMISSION RATE 

10 SECONDS 
32 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 6: Using the RS232 Receptacle 

following describes how to use an IBM compatible computer with 

Hyper TerminalTM software (included with Windows® 95, 98, XP 

or 2000 operating systems) to collect the RS232 data from your 

Freeze Dryer: 

1. Make sure that the Freeze Dryer is properly connected to the 

communication port on the computer. 

2. Open Hyper TerminalTM software. 

i)Windows® 95 or 98 use: 

START/PROGRAMS/ACCESSORIES. 

ii) Windows® 2000 or XP use: 

START/PROGRAMS/ACCESSORIES/COMMUNICATIONS 

The first time Hyper TerminalTM is opened a dialogue box 

requesting an area code and phone number will appear. Enter 

the appropriate numbers and continue. 

3. The “Connection Description” dialogue box will open. Type in 

a user defined name and select an icon for the new connection. 

Press “OK.” 

4. The “Connect To” dialogue box will open. Using the down 

arrow selection button, select the communication port to which 

the cable has been connected. Press “OK.” 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 33 

Chapter 6: Using the RS232 Receptacle 

5. The “Com X Properties” dialogue box will open. Enter the 

appropriate data properties and press “OK.” 
34 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 6: Using the RS232 Receptacle 

6. When the Freeze Dryer main power switch is on, the data will 

be transmitted and updated at the time intervals selected by the 

user. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 35 

CHAPTER 7

TROUBLESHOOTING

Refer to the following if your Freeze Dryer fails to operate 

properly. If the suggested corrective actions do not solve your 

problem, contact Labconco for additional assistance. 

Labconco Freeze Dry Systems that are clean, dry and without 

samples attached should reach a vacuum of 0.133 mBar within 10 

minutes and should achieve an ultimate vacuum of 0.033 mBar 

within 18 hours when the refrigeration is operating. If the Freeze 

Dryer does not obtain a satisfactory vacuum, perform the following 

maintenance tests. 

I. Vacuum Pump 

First make sure that the vacuum pump operates. If it fails to 

operate, check the electrical connections of the Freeze Dryer to the 

power source and then check the electrical connection of the 

vacuum pump to the Freeze Dryer. If the vacuum pump has a 

power switch, make sure that it is turned on. If the vacuum is not 

adequate when the vacuum pump is operating, proceed with the 

following steps: 

1. Check the oil and ensure it is clear and clean. If the oil 

looks cloudy or has any particulates, replace the pump oil. 

Sometimes it may be necessary to flush the pump with 

clean oil several times. To flush the pump, run the pump 5 

to 10 minutes to allow the oil to warm up. Drain the oil 

and refill with clean oil. Repeat as necessary. 

2. Check the oil level in the pump. Ensure it is filled to the 

correct level. 

3. Check vacuum hose connections from the pump to the 

Freeze Dryer and try running the unit. 
36 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 7: Troubleshooting 

If vacuum problems continue, consider obtaining a second vacuum 

gauge capable of reading a vacuum of 0.010 mBar. It is often 

useful in determining if the vacuum pump is operating properly 

and the vacuum sensor reading is accurate. 

4. Isolate the pump by disconnecting the vacuum hose from 

the Freeze Dryer. Deadhead the pump by inserting the 

vacuum sensor from a secondary vacuum gauge into the 

end of the vacuum hose and observe the vacuum reading 

obtained. Confirm that the pump is capable of achieving an 

ultimate vacuum less than 0.010 mBar or approximately 10 

microns. If an inadequate vacuum reading is obtained, the 

pump has most likely failed and may need to be replaced or 

rebuilt. 

II. Gaskets, Tubing, Connections, Sample 

Valves 

1. Check all sample valves on the drying chamber/manifold 

and ensure all valves are closed or in the vent position. 

Check to ensure all fittings are tight and sealed properly 

against the chamber. Inspect valves for cracks or cuts. 

2. Inspect gasket(s) for cracks or defects. Ensure the gasket is 

clean. Apply a thin coat of vacuum grease to the gasket. 

Keep in mind too much vacuum grease can actually cause 

leaks. 

3. Check the drain line and ensure the drain plug is installed 

and the tube appears to be in good condition. 

4. Check all rubber vacuum tubing for signs of deterioration 

or cracking. 

5. Check all connections and make sure they are secure and 

leak tight. 

6. When checking the sample valves, pull as much vacuum as 

possible. If a vacuum indication is displayed, wiggle or 

rotate the valves and watch the gauge for any fluctuations. 

Fluctuations can indicate a potential vacuum leak. If the 

valve seems to be in good condition, remove the valve and 

apply a thin coat of vacuum grease to the stem and the 

outside sealing surface of the valve body, and reinstall the 

valve. DO NOT APPLY VACUUM GREASE TO THE 

SAMPLE VALVE GASKETS, WHICH SEAL TO THE 

WALL OF THE DRYING CHAMBER. If the valve still 

seems to be the source of the problem, remove the valve 

and stopper the hole with a rubber stopper. Continue 

checking the other valves. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 37 

Chapter 7: Troubleshooting 

7. Make sure that the sample valve bulkhead nuts are tight. 

Either tighten by hand or use the wrench supplied with 

chambers. To use the wrench, first remove the knob and 

stem assembly and also the valve body. The pins on the 

wrench fit into the round holes of the nut. The nut should 

be snug, but avoid over-tightening. Replace the valve 

body, knob and stem assembly. 

The illustrations below show how the sample valve installs on 

a chamber and a manifold. 

Valve Assembly (Clear Chamber with Valves) 

Knob & Stem 

Assembly

Valve Body 
38 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 7: Troubleshooting 

III. System Components and Collection 

Chamber Isolation 

1. Remove the drying chamber or manifold. 

2. Leave the gasket on and turn a large freeze dry flask upside 

down to cover the connection port. 

3. Start the freeze dryer and observe the vacuum indication. 

If the vacuum indication is good, then the problem is in the 

drying chamber or manifold and you should return to 

Section II, Gaskets, Tubing, Connections, Sample Valves. 

Thrust Washer Valve Body Knob & Stem 

Assembly 

Trim Ring 

Manifold Assembly 

Knob & Stem 

Assembly 

Bulkhead Nut 

Gasket 

(No grease 

required) 

Bulkhead 

Fitting 

Valve Body 

Valve Assembly (Manifold) Valve Assembly (Stainless Steel Chamber) 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 39 

Chapter 7: Troubleshooting 

The following test allows you to check the pump and the 

connections from the pump to the vacuum tube. 

1. Place a rubber stopper in the vacuum port in the left side of 

the collector chamber and another in the drain port in the 

bottom. 

2. Restart the vacuum system and check your vacuum 

indication. Insufficient vacuum indicates a bad pump or a 

leak in the connections from the pump to the side of the 

chamber. 
40 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 7: Troubleshooting 

The following test is used to check the collection chamber 

integrity. The figure below points out potential areas to locate a 

leak: 

1. Remove the stopper from the side of the chamber and place 

a freeze dry flask over the connection port. 

2. Fill the collection chamber with approximately 2 inches of 

water, enough to cover all fittings. Replace the collection 

chamber lid and turn on the vacuum pump. Allow the 

vacuum pump to run for approximately 5 to 10 seconds. (If 

the vacuum pump is left on, the water will begin to boil and 

the test will not be effective.) Look inside the chamber for 

any bubbling while the pump is running and after you shut 

the pump off. 

3. If bubbling is observed around the bulkhead fittings, access 

the bottom of the chamber and remove the insulation. 

Tighten the appropriate fitting by placing a wrench on the 

fitting inside the chamber and use a second wrench to turn 

the nut on the bottom of the chamber. Be careful not to 

damage the evaporator coils or the brazed joints to the 

refrigeration system. Once the fitting is tightened, perform 

the test again. Should the bubbling still be present, loosen 

the bulkhead fittings and apply vacuum grease to the Orings, then reassembly and test. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 41 

Chapter 7: Troubleshooting 

4. If bubbling was observed on the chamber surface, the 

chamber must be replaced. This type of leak cannot be 

successfully repaired. The leading cause of a chamber 

surface leak is corrosive residue left in the chamber after 

use. 

Refrigeration Module Operation 

Under a no-load condition, the FreeZone Single Stage Freeze Dry 

System can achieve a collector temperature of –47°C or lower 

when the system is under vacuum, within 40 minutes when the 

vacuum pump is running, depending on ambient temperature and 

humidity. 

If any repairs are required on the refrigeration module, contact 

your local laboratory supply dealer. If your laboratory supply 

dealer is unable to provide assistance, please call Labconco. 

Repairs should only be undertaken by a competent refrigeration 

technician or through an authorized Labconco service agency. 
42 Product Service Domestic 1-800-522-7658, International 816-333-8811 

CHAPTER 8

MODIFYING YOUR

FREEZE DRYER

DISPLAY

Your Freeze Dryer has been carefully calibrated and tested before 

shipping, however under certain circumstances it may be necessary 

to adjust the calibration of the temperature and/or vacuum display 

or to modify alarm points. Adjustments may be necessary if the 

Freeze Dryer requires service or if it is operating on a line voltage 

outside the voltage range at which the alarm was preset. This 

DOES NOT mean that the Freeze Dryer may be operated at a 

voltage outside the ranges shown in Appendix C. 

Temperature and Vacuum Display 

An offset may be added to the collector temperature or vacuum 

display. Use an accurate traceable reference to compare the 

readings. 

Temperature offset may be varied ±5°C in 1°C increments. 

Vacuum offset may be varied ±0.020 mBar in 0.001 mBar 

increments. 

Line Voltage Alarm Points 

The Freeze Dryer is designed to alarm if the line voltage varies 

more than ±10% from nominal line voltage. Models rated to 

operate on 115V are preset to alarm if the voltage drops below 

approximately 103V or rises above approximately 127V. Models 

rated to operate on 230V will alarm if the voltage drops below 

approximately 206V or rises above approximately 254V. Some 

models are designed to operate below these ranges (see Appendix 

C). In these cases it is permissible to lower the low voltage alarm 

point to prevent nuisance alarms from occurring. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 43 

Chapter 8: Modifying Your Freeze Dryer Display 

Low line limit may be lowered in 5 increments of 

approximately 2 to 3V increments for 115V models or 

disabled. 

Low line limit may be lowered in 5 increments of 

approximately 4 to 5V increments for 230V models or 

disabled. 

High line limit may be raised in 5 increments of 

approximately 2 to 3V increments for 115V models or 

disabled. 

High line limit may be raised in 5 increments of 

approximately 4 to 5V increments for 230V models or 

disabled. 

Moisture Sensor Alarm 

The moisture sensor alarm may be disabled. It is shipped enabled 

as the factory default setting. 

Modifying the Display 

To enter an offset or to configure the alarms, perform the following 

steps: 

Press and hold MENU button (approximately 10 seconds) 

until the display shows: 

It is recommended that you do NOT perform a Master 

Reset because all offsets and configurations that have been 

entered will be lost. If you must perform a Master Reset, 

hold SELECT approximately 5 seconds. 

This will reset: 

Vacuum units to mBar 

Vacuum set point to 0 mBar 

Temperature units to °C 

Refrigeration total hours to 0 

Vacuum pump total hours to 0 

RS232 transmission rate to 10 seconds 

Temperature offset to 0°C 

Vacuum offset to 0 mBar 

Low line voltage to 0 V offset 

High line voltage to 0 V offset 

Moisture sensor to enabled 

Refrigeration to operate in Manual Mode 

PRODUCTION MENU 

MASTER RESET?
44 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 8: Modifying Your Freeze Dryer Display 

The display will default to the main freeze dry display information. 

If you do not want to restore all factory settings, but want to adjust 

offsets, press MENU briefly and the display will show: 

Press SELECT until the appropriate offset is shown. Each 

number represents 1°C 

Press MENU and if the drying chamber has been installed, the 

display will show: 

Press SELECT until the appropriate offset is shown. Each number 

represents 1°C 

Press MENU and the display will show 

Press SELECT until the appropriate offset is shown. Each 

number represents 0.001mBar. 

Press MENU and the display will show 

Press SELECT until the appropriate offset is shown. If the 

Freeze Dryer is operating on nominal 115V, each number 

represents 2V. If 3 is entered into the display, the low line 

alarm will alarm when the voltage drops to approximately 

97V, for example 103-(2x3)=97V. If the Freeze Dryer is 

operating on nominal 230V each number represents 4V (if 

3 is entered into the display, the low line alarm will alarm 

when the voltage drops to approximately 194V for example 

206-(4x3)=194V. 

There will be no alarm indicated if DISABLED is selected. 

Press MENU and the display will show 

COLLECTOR TEMP OFFSET 

O C

SDC TEMP. OFFSET: 

O C 

VACUUM OFFSET: 

O uBar 

LOW LINE OFFSET: 

0 

HIGH LINE OFFSET: 

0 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 45 

Chapter 8: Modifying Your Freeze Dryer Display 

Press SELECT until the appropriate offset is shown. If the 

Freeze Dryer is operating on nominal 115V, each number 

represents 2V. If 3 is entered into the display, the high line 

alarm will alarm when the voltage raises to approximately 

133V, for example. 127+(2x3)=133V. If the Freeze Dryer 

is operating on nominal 230V each number represents 4V 

(if 3 is entered into the display, the low line alarm will 

alarm when the voltage drops to approximately 266V, for 

example. 254+(4x3)=266V. There will be no alarm 

indicated if DISABLED is selected. 

Press MENU and the display will show 

Press and hold SELECT to change the status. 

Press MENU and the display will show 

Press and hold SELECT to change the status. 

Press MENU and the display will show 

Press and hold SELECT to change the status. 

Press MENU and the display will show 

Press and hold SELECT if you want to disable the 

moisture sensor. 

Press MENU and the display will show 

Press and hold SELECT to change the status 

DEFROST HEATER: 

NOT INSTALLED 

SHELL FREEZER: 

NOT INSTALLED 

DRYING CHAMBER: 

NOT INSTALLED 

PURGE VALVE: 

NOT INSTALLED 

MOISTURE SENSOR: 

INSTALLED 
46 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Chapter 8: Modifying Your Freeze Dryer Display 

Press MENU and the display will show 

If you want to reset Total Hours, press and hold SELECT until an 

audible beep is heard. The display will default to the main freeze 

dry display information. 

If you do not want to set Total Hours, press MENU and the display 

will show. 

The routine will repeat by pressing MENU or wait approximately 

10 seconds for the display to default to the main freeze dry 

information display information. 

In order to store offsets in memory, you Must Press and Hold 

MENU until display shows, 

Wait approximately 10 seconds and the display will default to the 

main freeze dry display information. This can be done anytime 

throughout the Modifying the Display routine. 

HOLD SELECT TO CLEAR 

ALL TOTAL HOURS? 

PRODUCTION MENU: 

MASTER RESET? 

PASSWORD: 
47 Product Service Domestic 1-800-522-7658, International 816-333-8811 

APPENDIX A

FREEZE DRYER

COMPONENTS

The following pages list components that are available for your 

Freeze Dryer. The parts shown are the most common replacement 

parts. If other parts are required, contact Product Service. 
48 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Appendix A: Freeze Dryer Components 

Item Part No. Description 

1 7690800 Lid Gasket 

2 7532900 Lid 

3 7448000 Printed Circuit Board RS232 

4 7953800 Motor Fan, 115V 

 7953801 Motor Fan, 230V 

5 7750602 Compressor, 115V, 60 Hz 

 7750601 Compressor, 230V, 50 Hz 

 7750600 Compressor, 230V, 60 Hz 

6 1302300 Switch 

7 7426000 Control Panel Label 

8 7445590 Printed Circuit Board 

9 7728000 Drain Plug 

10 1643600 O-Ring 

11 7645904 Drain Hose 

12 7646000 Vacuum Hose (Not Shown) 

13 1336400 Power Cord 115V (Not Shown) 

 1342100 Power Cord, 230V (Not Shown) 

14 7445400 Vacuum Sensor 

15 7515300 Temperature Sensor (Not Shown) 

16 7400502 Wire Harness, Power, 115V (Not Shown) 

 7400503 Wire Harness, Power, 230V (Not Shown) 

17 7432700 Vacuum Control/Bleed Valve 

18 7429700 Moisture Sensor 

19 7418400 Wire Harness, Control 115V and 230V (Not Shown) 

20 1289320 Circuit Breaker 115V 20A 

 1289310 Circuit Breaker 230V 10A 

21 1289200 Relay – Vacuum Pump 115V 

 1289100 Relay – Vacuum Pump 230V 

22 1289200 Relay-Refrigeration 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 49 

Appendix A: Freeze Dryer Components 
50 Product Service Domestic 1-800-522-7658, International 816-333-8811 

APPENDIX B

FREEZE DRYER

DIMENSIONS
 Product Service Domestic 1-800-522-7658, International 816-333-8811 51 

APPENDIX C

FREEZE DRYER

SPECIFICATIONS

This Appendix contains technical information about the Freeze 

Dryer including electrical specifications, environmental operating 

conditions and wiring diagrams. 

Electrical Specifications 

Catalog # Voltage Frequency Phase Amperage 

Nominal Operating 

Range 

 Nominal 

w/o 

Vacuum 

Pump 

Max. with 

Vacuum 

Pump 

7752020 115V 103-127 60 1 9 16 

 100V 90-110 50 1 9 16 

7752021 115V 103-127 60 1 9 16 

 100V 90-110 50 1 9 16 

7752030 230V 198-254 50 1 3 8 

7752031 230V 198-254 50 1 3 8 

7752040 230V 187-253 60 1 3 8 

7752041 230V 187-253 60 1 3 8 
52 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Appendix C: Freeze Dryer Specifications 

Environmental Conditions 

• Indoor use only. 

• Maximum altitude: 6562 feet (2000 meters). 

• Ambient temperature range: 41° to 104°F (5° to 40°C). 

• Maximum relative humidity: 80% for temperatures up to 

88°F (31°C), decreasing linearly to 50% relative humidity 

at 104°F (40°C). 

• Main supply voltage fluctuations not to exceed ±10% of the 

nominal voltage. 

• Transient over voltages according to Installation Categories 

II (Over voltage Categories per IEC 1010). Temporary 

voltage spikes on the AC input line that may be as high as 

1500V for 115V models and 2500V for 230V models are 

allowed. 

• Used in an environment of Pollution degrees 2 (i.e., where 

normally only non-conductive atmospheres are present). 

Occasionally, however, a temporary conductivity caused by 

condensation must be expected, in accordance with IEC 

664. 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 53 

Appendix C: Freeze Dryer Specifications 

Wiring Diagram Catalog # 7752020 and 7752021, 

(115V, 60 Hz Models)
54 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Appendix C: Freeze Dryer Specifications 

Wiring Diagram Catalog #7752030, 7752031, 7752040 

7752041 (230V, 50 or 60 Hz Models) 
 Product Service Domestic 1-800-522-7658, International 816-333-8811 55 

APPENDIX D

FREEZE DRYER

ACCESSORIES

PART # DESCRIPTION 

1467700 Vacuum Pump 

Two stage direct drive pump, 195 liters/minute. 115 VAC, 

50/60 Hz, single phase, 7.8 amps. Includes Pump Exhaust Filter 

1473400. (Catalog #1473400) 

7739403 Vacuum Pump 

Two stage direct drive pump, 195 liters/minute. 230 VAC, 

50/60 Hz, single phase, 4.0 amps. Includes Pump Exhaust Filter 

1473400. (Catalog #1473400) 

7439000 Vacuum Pump 

Two stage direct drive pump, 173 liters/minute. 115 VAC, 

50/60 Hz, single phase 5.8 amps. Includes Pump Exhaust Filter. 

(Catalog #7670400) 

7439200 Vacuum Pump 

Two stage direct drive pump, 173 liters/minute. 230 VAC, 

50/60 Hz, single phase 2.9 amps. Includes Pump Exhaust Filter. 

(Catalog #7670400) 

7769600 Vacuum Pump, Chemical Resistant 

Two stage direct drive pump, 173 liters/minute. 115 VAC, 

50/60 Hz, single phase 5.8 amps. Includes Pump Exhaust Filter. 

(Catalog #7670400)

7769800 Vacuum Pump, Chemical Resistant 

Two stage direct drive pump, 173 liters/minute. 115 VAC, 

50/60 Hz, single phase 2.9 amps. Includes Pump Exhaust 

Filter.(Catalog #7670400)

The following accessories are available for the Freeze Dryer. 
56 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Appendix D: Freeze Dryer Accessories 

PART # DESCRIPTION 

1472200 Pump Inlet Filter 

Disposable filter that prevents oil back streaming and protects vacuum 

pump from sub micron particles. Fits vacuum pumps 1466700 and 

7739403. 

1473400 Pump Exhaust Filter 

Disposable filter that removes visible oil mist and odor from vacuum 

pump exhaust. Fits vacuum pumps 1467700 and 7739403. 

1473200 Replacement Element, Oil Mist, Pump Exhaust Filter 

Fits pump exhaust filter 1473400. 

1473300 Replacement Element, Odor, Pump Exhaust Filter, 

package of 5 

Fits pump exhaust filter 1473400 or vacuum pumps 1467700 and 

7739403. 

7670400 Replacement Filter – Element 

Disposable filter that removes oil mist from the vacuum pump exhaust. 

Fits vacuum pumps 7439000, 7439200, 7769600 and 7769800 

1988000 Vacuum Pump Oil, 1 Liter 

A molecularly distilled hydrocarbon oil with low vapor pressure. 

For vacuum pumps 1467700 and 7739403 

7772000 Soda Acid Trap 

Secondary trap that prevents migration of corrosive chemicals into 

vacuum pump. 

7772100 Replacement Media for Soda Acid Trap 

7772700 Vacuum Pump Oil 1 Liter 

A mineral oil with low vapor pressure. 

For vacuum pumps 7439000, 7439200, 7769600, 7769800 

7772500 Carbon Solvent Trap 

Secondary trap that prevents migration of organic solvents into vacuum 

pump. 

7772600 Replacement Activated Carbon Media for Carbon Solvent Trap 
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 57 

Appendix D: Freeze Dryer Accessories 

PART # DESCRIPTION 

7538000 Secondary Vacuum Cold Trap 

Provides additional protection for the vacuum pump when 

processing low eutectic samples. 

9 3⁄4" high x 7 7/8" diameter, 304 stainless steel with 3⁄4" vacuum 

connections. For use with dry ice and solvent. Cools to 

approximately –75°C. 

7522800 12 Port Drying Chamber 

9 3⁄4" high x 7 7/8" diameter, 304 stainless steel with 12 freeze dry 

valves. 

7522900 16 Port Drying Chamber 

13" high x 138" diameter, 304 stainless steel with 16 freeze dry 

valves. 

7443500 Clear Drying Chamber with 8 Valves 

For bulk drying or drying in flasks. 9.0 Dia. 

7444000 Clear Drying Chamber with 8 Valves 

For bulk drying or drying in flasks. 12.0 Dia.

7442900 Clearing Drying Chamber 

For bulk drying. 9.0 Dia. 

7867000 Clear Drying Chamber 

For Bulk Drying 12.0 Dia. 

7521000 Heated Drying Chamber 

10 1⁄2" high x 9" diameter type 304 stainless steel. The 3 shelf 

product heaters heat to 43°C (110°F). 115V. 

7521001 Heated Drying Chamber 

10 1⁄2" high x 9" diameter type 304 stainless steel. The 3 shelf 

product heaters heat to 43°C (110°F). 230V. 

7509200 Product Heater 

For use in chamber 7522800. Provides 3 heated shelves operating 

at 43°C (110°F). 115V 

7509201 Product Heater 

For use in chamber 7522800. Provides 3 heated shelves operating 

at 43°C (110°F). 230V 

7442100 Drying Rack- Unheated 

Provides three shelves. For use in chambers 7443500 and 

7522800. 

7441700 Drying Rack – Unheated 

Provides three shelves for use in chambers 7444000, 7867000, 

7522900

7522300 20 Port Manifold 

10" high x 27 1⁄2" wide x 8 5/8" deep. Type 304 stainless steel 

manifold with 20 freeze dry valves. 
58 Product Service: Domestic 1-800-522-7658, International 816-333-8811 

Appendix D: Freeze Dryer Accessories 

PART # DESCRIPTION 

7522400 10 Port Manifold with support shelf 

10" high x 26" wide x 6-5/8" deep. Type 304 

Stainless Steel Manifold with 10 Freeze Dry Valves 

& 1 Shelf

7522500 20 Port Manifold with support shelves 

10" high x 26" wide x 9-1/2" deep. Type 304 

Stainless Steel Manifold with 20 Freeze Dry valves 

and 2 shelves. 

7868500 48 Port Ampule Manifold 

28 5/8" high x 5" wide x 5" deep. Type 304 

stainless steel with 

48 each 1⁄4" tube stems. 

7509600 Sample Valve Kit 

Includes neoprene valve body, knob and installation 

parts. 

7439901 Glass Collector Chamber Lid 

For use with solvlents that attack the acrylic lid. 

7439902 Glass Collector Chamber Lid 

For use with solvlents that attack the acrylic lid.

7509300 Product Heater 

For use in chamber 7522900, 7444000 and 

7867000. Provides 3 heated shelves operating at 

43°C (110°F). 115V 

7509301 Product Heater 

For use in chamber 7522900, 7444000 and 

7867000. Provides 3 heated shelves operating at 

43°C (110°F). 230V 

7509400 Product Heater with Variable Heat 

For use in chamber 7522900, 7444000 and 

7867000. Provides variable heat up to 43°C 

(110°F). 115V 

7509401 Product Heater with Variable Heat 

For use in chamber 7522900, 7444000 and 7867000

7806020 Bulk Tray Dryer 

115V, 60 Hz, three shelves, 630 square inches of 

area, provides heat control 

7806021 Bulk Tray Dryer 

115V, 60 Hz, three shelves, 630 square inches of 

area, provides heat control with 6 port manifold

7806022 Bulk Tray Dryer 

115V, 60 Hz, three shelves, 630 square inches of 

area, provides heat control with RS232 output 

connection.
 Product Service: Domestic 1-800-522-7658, International 816-333-8811 59 

Appendix D: Freeze Dryer Accessories 

PART # DESCRIPTION 

7806023 Bulk Tray Dryer 

115V, 60 Hz, three shelves, 630 square inches of area, provides 

heat control with 6 port manifold and RS232 output connection.

7806030 Bulk Tray Dryer 

230V, 50/60 Hz, Three shelves, 630 square inches of area, 

provides heat control. 

7806031 Bulk Tray Dryer 

230V, 50/60 Hz, Three shelves, 630 square inches of area, 

provides heat control. With 6 port manifold.

7806032 Bulk Tray Dryer 

230V, 50/60 Hz, Three shelves, 630 square inches of area, 

provides heat control. With RS232 output connection.

7806033 Bulk Tray Dryer 

230V, 50/60 Hz, Three shelves, 630 square inches of area, 

provides heat control. With 6 port manifold and RS232 output 

connection.

7948020 Stoppering Tray Dryer 

115V, 60 Hz, three shelves, 600 square inches of area, provides 

temperature control and stoppering under vacuum. 

7948030 Stoppering Tray Dryer 

230V, 50 Hz, three shelves, 600 square inches of area, provides 

temperature control and stoppering under vacuum. 

7948040 Stoppering Tray Dryer 

230V, 60 Hz, three shelves, 600 square inches of area, provides 

temperature control and stoppering under vacuum.

7726500 6 Port Manifold for use with Stoppering Tray Dryer 

Mounts between the Stoppering Tray Dryer and the base unit 

and provides 6 freeze dry valves. 

7537800 Cable 

Connect the RS232 output from the Freeze Dryer (or Freeze 

Dryer/Tray Dryer combination) to an IBM compatible computer 

with a 9-pin serial data port. 

7537801 Cable 

Connects the RS232 output from the Freeze Dryer (or Freeze 

Dryer/Tray Dryer combination) to an IBM compatible computer 

with a 25-pin serial data port. 

7761500 Isolation Valve 

Mounts between the Stoppering Tray Dryer and the base unit 

which provides a way to isolate the base unit vacuum system 

from the Stoppering Tray Dryer vacuum system. 

7390702 Baffle 

Mounts in the collector chamber to increase ice holding capacity 

when operating with the vacuum control. 
Fast-Freeze Flasks are specially designed to be easier to

handle, faster to load and more convenient to use than other

freeze dry glassware now in your laboratory. Compatible

with all major brands of laboratory freeze dry equipment,

Fast-Freeze Flasks eliminate the risk of contamination from

vacuum grease, reduce spillage of valuable samples and

require no washers, gaskets or retainers.

How to select Fast-Freeze Flasks for

your Freeze Dry System

Select Fast-Freeze Flasks based on your sample sizes. Flasks

should be filled no more than one-third of their volume so

that maximum surface area is achieved and efficient

lyophilization is assured. A complete Fast-Freeze Flask

includes a rubber top, glass bottom and a supply of filter

paper. Tops, bottoms and filter paper are available separately as replacement components. Adapters are required to

attach flasks to freeze dry valve ports.

Adapters

Add the Adapters for connecting

the Fast-Freeze Flasks to the valve

ports on your drying chamber or

manifold. Choose borosilicate glass

or stainless steel adapters in 1/2"

and 3/4" diameters.

75448-10 Replacement Filter Paper

1000 (10 packages of 100). 1" diameter (28mm). Pore size 17 microns.

Shipping weight 0.5 lb.

*Valves on the freeze dry systems, drying chambers and manifolds offered in this catalog fit adapters

of both 1/2” and 3/4" sizes. Size information is provided for selecting adapters for older Labconco models and other freeze dryers.

Fast-Freeze® Flasks

Variety of adapters

available. You may

select from glass or

stainless steel

adapters, available

straight or with 45°

bend. (Required —

order separately.)

No complex filter

retainer is necessary.

For those who use

filters, they are easily

inserted between the

adapter and top of

the flask. One hundred filters are supplied with each flask.

Compatible with

all major brands of

laboratory freeze dry

apparatus.

Wide mouth

opening loads fast,

cleans easily.

Horizontal shell

freezing. Flask

may be placed in

a horizontal position and rotated

in a bath for shell

freezing.

Available in nine

convenient sizes.

Flat bottom

reduces spills,

provides better

balance.

Top seals easily. The

flexible silicone rubber

top snaps on and off

easily, yet provides a

reliable, high vacuum

seal.

No vacuum

grease is

necessary.

No threads,

hooks or springs

are needed.

Entire flask may

be autoclaved.

Only two pieces

per flask.

The complete

Fast-Freeze Flask

has only two

components: 

• a high strength

borosilicate

glass bottom 

to withstand

extreme 

temperatures

and high

vacuum.

• a flexible, noncontaminating

silicone rubber

top.

Flask Complete Flask Flask Flask Top Dimensions/ 

Size Flask Bottom Top Adapter Flask Bottom

Diameter* H x D

40 ml 7540000 7542000 7544000 1/2" 76 mm x 34 mm

80 ml 7540200 7542200 7544000 1/2" 115 mm x 34 mm

120 ml 7540300 7542300 7544200 3/4" 68 mm x 59.2 mm

150 ml 7540400 7542400 7544200 3/4" 85 mm x 59.2 mm

300 ml 7540600 7542600 7544200 3/4" 145 mm x 59.2 mm

600 ml 7540800 7542800 7544400 3/4" 135 mm x 90.2 mm

900 ml 7540900 7542900 7544400 3/4" 190 mm x 90.2 mm

1200 ml 7541000 7543000 7544400 3/4" 240 mm x 90.2 mm

2000 ml 7541200 7543200 7544400 3/4" 380 mm x 90.2 mm

Diameter Straight 45° Bend

Adapter Adapter

Borosilicate Glass

1/2" flask top to 1/2" valve* 7545000 7545600

3/4" flask top to 3/4" valve* 7545200 7545800

1/2" flask top to 3/4" valve* 7545400 7546000

3/4" flask top to 1/2" valve* 7545400 7546000

Stainless Steel

1/2" flask top to 1/2" valve* 7547000 7547400

3/4" flask top to 3/4" valve* 7547200 7547600

Product Service Domestic 1-800-522-7658, International 816-333-8811

Appendix D: Freeze Dryer Accessories

60
Lyph-Lock Flasks simplify your lyophilization procedures

because they have only three pieces per flask — a high

strength borosilicate glass top and bottom with a silicone

rubber ring seal. The unique wide-mouth design helps you

load samples easily and lyophilize efficiently.

How to select Lyph-Lock Flasks for

your Freeze Dry System

Select the Lyph-Lock Flasks based on your sample sizes.

Flasks should be filled to no more than one-third of their

volume so that maximum surface area is achieved and efficient lyophilization is ensured. A complete Lyph-Lock Flask

includes a glass top and bottom and a rubber ring seal. Tops,

bottoms and seals are available separately as replacement

components. Adapters are required to attach flasks to freeze

dry valve ports.

90° Bend Description

Adapter

7568000 Connects 19/38 STJ Flask Top to 1/2" valve*

7568200 Connects 19/38 STJ Flask Top to 3/4" valve*

7568400 Connects 24/40 STJ Flask Top to 1/2" valve*

7568600 Connects 24/40 STJ Flask Top to 3/4" valve*

*Valves on the freeze dry systems, drying chambers and manifolds offered in this catalog fit adapters

of both 1/2” and 3/4" sizes. Size information is provided for selecting adapters for older Labconco

models and other freeze dryers.

Borosilicate glass

adapters with

either 19/38 or 24/40

standard taper joints

have 90° bend.

(Required — order

separately.)

Silicone rubber

seal grasps top

and bottom.

The flexible ring

holds securely

together during

pre-freezing

and lyophilization.

Compatible with

all major

brands of laboratory freeze

dry apparatus.

Flat bottom

reduces spills,

provides better

balance.

Available

in seven

convenient sizes.

Entire flask may

be autoclaved.

Provides a high

vacuum seal for

efficient

lyophilization.

Only three pieces

per flask — no

hooks or springs.

The complete

Lyph-Lock Flask

includes: 

• a flask top of

high strength

borosilicate

glass

• a flexible, noncontaminating

silicone rubber

seal

• a flask bottom

of borosilicate

glass to withstand extreme

temperatures

and high

vacuum.

Adapters

Add the Adapters for connecting the Lyph-Lock Flasks to the

valve ports on your drying chamber or manifold.

Flask Complete Complete Flask Flask Lyph-Lock Flask Dimensions

Size Flask Flask Top Top Seal Bottom Flask Bottom

19/38 STJ 24/40 STJ 19/38 STJ 20/40 STJ H x ID

25 ml 7550000 7554000 7552000 7556000 7559000 7557000 37 mm x 34 mm

50 ml 7550200 7554200 7552000 7556000 7559000 7557200 67 mm x 34 mm

100 ml 75504-00 7554400 7552200 7556200 7559200 7557400 50 mm x 59.2 mm

250 ml 75506-00 7554600 7552200 7556200 7559200 7557600 110 mm x 59.2 mm

500 ml 7550800 7554800 7552400 7556400 7559400 7557800 103 mm x 90.2 mm

750 ml 7550900 7554900 7552400 7556400 7559400 7557900 145 mm x 90.2 mm

1000 ml 7551000 7555000 7552400 7556400 7559400 7558000 187 mm x 90.2 mm

Lyph-Lock® Flasks

Appendix D: Freeze Dryer Accessories

Product Service Domestic 1-800-522-7658, International 816-333-8811 61
Accessories

Seal Crimper

Secures tear-away Aluminum Seals.

7578000 Seal Crimper for 13 mm corkage. 

Shipping weight 3 lbs. (1.4 kg).

7578100 Seal Crimper for 20 mm corkage. 

Shipping weight 3 lbs. (1.4 kg).

Vacuum Stoppering Adapter

7593000 Adapter connects to valve ports for manual stoppering of 20 mm corkage serum bottles under original vacuum. The stopper and serum bottle are inserted into the

adapter body and lyophilization begins. After the process is

completed, the researcher slides the stopper into position

so when the valve is turned from vacuum to vent the serum

bottle is tightly sealed by the stopper. The ejector tube is

then used to force the serum bottle out of the adapter. 

Shipping weight 1 lb. (0.4 kg).

Perfect for long term storage of freeze dried samples.

Labconco Serum Bottles and Threaded Vials are specifically

designed for lyophilization applications. Their uniform thin

wall construction ensures even freezing and drying. Bottles

and vials are ideal containers for use in the FreeZone

Stoppering Tray Dryer. Serum bottles also connect to valve

ports on drying chambers and manifolds.

Serum Bottles

Serum Bottles, Stoppers and Seals are supplied in packages of 100.

Threaded Vials

Stoppers and Threaded Vials with Screw Caps are supplied

in packages of 200.

Serum Bottles and Vials

Size 20 mm 13 mm Split Aluminum Sleeve-Type 

Corkage Corkage Stoppers Seals Stoppers

2 ml 7575010 7576010 7577010

3 ml 7575210 7576010 7577010

5 ml 7573010 7576210 7577110 7577510

10 ml 7573210 7576210 7577110 7577510

20 ml 7573410 7576210 7577110 7577510

30 ml 7573610 7576210 7577110 7577510

50 ml 7573810 7576210 7577110 7577510

100 ml 7574010 7576210 7577110 7577510

125 ml 7574210 7576210 7577110 7577510

Size Vials with Stoppers

Screw Caps

5 ml 7762300 7762200

10 ml 7762600 7762200

Stoppering Adapter Body Serum Bottle

Vent Hole

Valve Port

Ejector Tube Stopper

Product Service Domestic 1-800-522-7658, International 816-333-8811

Appendix D: Freeze Dryer Accessories

62
Accessories

Oxygen/Natural Gas Sealing Torch

7578500 Torch specifically designed for flame sealing freeze

dry ampules. Seals all types of heat-resistant glass. Connects

to natural gas, butane or propane and oxygen with 1/4" ID

hose connectors. Shipping weight 3 lbs. (1.4 kg). 

Labconco Ampules are fabricated of highest quality borosilicate glass for strength and durability. They are available in

five different configurations to provide the ideal ampule for

your specific lyophilization requirements, from tray drying to

flame sealing applications.

Ampules

Ampules are supplied in packages of 100.

Ampules

Size Straight Bulb-Type Pre-Scored Round Stoppering Flat Bottom Bottom

1 ml 7572710 7570010 757010

2 ml 7570810 7572310

4 ml 757210

5 ml 7570210 7571010

10 ml 757110 7571610

25 ml 7571810

50 ml 7572010

Ampule Valve Adapters

7593401 Adapter connects ampules to valve ports or stainless

steel stems. Ten per package. Shipping weight 0.5 lb. (0.2 kg).

Stoppers

7572510 Stopper for Stoppering Ampules 75723-10.

100 per package. Shipping weight 3 lbs. (1.4 kg).

Trident Adapter

7762700 Three Way Adapter permits attachment of three

ampules to a single valve port. Ampules are attached using

Ampule Valve Adapters or 1/8" surgical tubing (not provided).

Cavity in adapter body can be filled with cotton fiber media

to help prevent contamination between samples.

Shipping weight 3 oz. (0.1 kg).

Appendix D: Freeze Dryer Accessories

Product Service Domestic 1-800-522-7658, International 816-333-8811 63
64 Product Service Domestic 1-800-522-7658, International 816-333-8811 

DECLARATION OF CONFORMITY 

Application Council Directive(s): 73/23/EEC, 89/336/EEC 

Standard(s) to which conformity is declared: EN61010, EN55022, EN50082-1 

Manufacturer’s Name: Labconco Corporation 

Manufacturer’s Address: 8811 Prospect Avenue 

 Kansas City, MO 64132 USA 

Importer’s Name: See Shipping/Customs Documents* 

Importer’s Address: See Shipping/Customs Documents for your equipment 

Type of Equipment: Laboratory Equipment – Freeze Dryer 

Model No.: 775 followed by two digit number based on model size; followed by 

another two digit number based on options. 

 77400 followed by two digit number based on options. 

 79340 followed by two digit number based on options. 

767 followed by two digit number based on model size; followed by 

another two digit number based on options. 

 79600 followed by two digit number based on options. 

 74200 followed by two digit number based on options. 

Serial No.: Various – See Individual Declaration 

Year of Manufacture: 1995 and Subsequent 

I, the undersigned, hereby declare that the equipment specified above conforms to the 

above Directive(s) and Standard(s). 

 

 See individual Declaration of Conformity which 

 will be signed by the importer for your country. 

Place: _______________________________________ 

 (Signature) 

Date: _______________________________________ 

 (Full Name) 

 _______________________________________ 

 (Position) 

*An individual version of this declaration is included with your shipping/customs 

documentation. 

Labconco P/N 36960-02, Rev. E, ECO C010