Labconco Corporation 

8811 Prospect Avenue 

Kansas City, MO 64132-2696 

800-821-5525, 816-333-8811 

FAX 816-363-0130 

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User’s Manual

Precise® HEPA-Filtered 

Glove Boxes 

Models Voltage, Frequency 

5220200 115V, 60 Hz 

5220220 230V, 50 Hz 

5220221 230V, 60 Hz 

5220230 100V, 50 Hz 

5220231 100V, 60 Hz 

Please read the User’s Manual before operating the equipment. 
Copyright © 2007, 2008 Labconco Corporation. All rights reserved. 

The information contained in this manual and the accompanying products are copyrighted and all 

rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic 

design changes without obligation to notify any person or entity of such change. 

Returned or Damaged Goods 

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be 

accepted. If your shipment was damaged in transit, you must file a claim directly with the freight 

carrier. Labconco Corporation and its dealers are not responsible for shipping damages. 

The United States Interstate Commerce Commission rules require that claims be filed with the 

delivery carrier within fifteen (15) days of delivery. 

Limitation of Liability 

The disposal and/or emission of substances used in connection with this equipment may be governed 

by various federal, state, or local regulations. All users of this equipment are required to become 

familiar with any regulations that apply in the user’s area concerning the dumping of waste materials 

in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held 

harmless with respect to user’s compliance with such regulations. 

Contacting Labconco Corporation 

If you have questions that are not addressed in this manual, or if you need technical assistance, contact 

Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-

5525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time. 

Part #5233702, Rev. A 

ECO E766 

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of 

defective material and workmanship, provided such defect results from normal and proper use of the 

equipment. 

The warranty for all Labconco products will expire one year from date of installation or two years 

from date of shipment from Labconco, whichever is sooner, except the following; 

• Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Lab Animal Research 

Stations carry a three-year warranty from date of installation or four years from date of 

shipment from Labconco, whichever is sooner. 

• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from 

date of installation or three years from date of shipment from Labconco, whichever is 

sooner. 

• Blood Drawing Chairs carry a ten year warranty. 

• Carts carry a lifetime warranty. 

• Glassware is not warranted from breakage when dropped or mishandled. 

This limited warranty covers parts and labor, but not transportation and insurance charges. In the 

event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If 

the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair 

or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco 

Corporation be liable for indirect, consequential, or special damages of any kind. This statement 

may be altered by a specific published amendment. No individual has authorization to alter the 

provisions of this warranty policy or its amendments. Lamps and filters are not covered by this 

warranty. Damage due to corrosion or accidental breakage is not covered.
TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION 1 

 

CHAPTER 2: PREREQUISITES 2 

 Support, Vibration & Preventive Requirements 3 

 Location Requirements 3 

 Exhaust and Blower Requirements 3 

 ISO Class 3 Definition 5 

 Electrical Requirements 7 

 Space Requirements 7 

CHAPTER 3: GETTING STARTED 8 

 Unpacking the Glove Box 9 

 Installing the Glove Box on a Supporting Structure & 

 Work Surface 10 

 Connecting to the Exhaust System (If Applicable) 10 

 Installation of Gloves to the Glove Ports 12 

 Connecting the Electrical Supply to the Glove Box 13 

 Setting Exhaust Volume with Speed Control Adjustment 13 

 Validating the Glove Box 14 

 Summary of Performance Testing Results 14 

CHAPTER 4: PERFORMANCE FEATURES AND 

 SAFETY PRECAUTIONS 16 

 Performance Features 16 

 Safety Precautions 19 

 

CHAPTER 5: USING YOUR GLOVE BOX 21 

Routine Daily Work Procedures 21 

 HEPA Filter Applications, Suitability & Guidelines 23 

 Definition of Terms 24 

 Appropriate Chemicals for Carbon Filters 24 

 Hazardous Misapplications for Carbon Filters with 

 Volatile Chemicals 25 

 Chemical Carcinogen Use with Carbon Filters 25 

 Prohibited Acid Use 26 
CHAPTER 6: MAINTAINING YOUR GLOVE BOX 27 

 Routine Maintenance Schedule 28 

 Decontamination 29 

 Determining When to Replace HEPA Filters 29 

 How to Install New HEPA Filters 29 

 HEPA Filter Leak Test 31 

 Initial Certification 33 

 Re-Certification 33 

 Fluorescent Light Replacement 33 

 Motorized Impeller Replacement 34 

 Speed Control Replacement 34 

CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 35 

CHAPTER 8: TROUBLESHOOTING 41

APPENDIX A: REPLACEMENT PARTS 42 

APPENDIX B: DIMENSIONS 44 

APPENDIX C: SPECIFICATIONS 45 

APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 46 

APENDIX E: PRESSURE & FLOW RATE CONVERSIONS 48 

APPENDIX F: REFERENCES 49

DECLARATION OF CONFORMITY 50 
Product Service 1-800-522-7658 1

Chapter 1:

Introduction

Congratulations on your purchase of a Labconco Precise® HEPA-Filtered 

Glove Box. Your glove box provides personnel protection through superior 

containment rated at Class 1 conditions for hazardous environments, per 

ISO 10648-2. 

These glove boxes will effectively contain toxic or noxious particulates when 

properly installed and operated. In positive or negative pressure operation 

modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean 

air environment. This glove box uses two HEPA filters, rated at least 99.99% 

efficient for 0.3-micron particles. 

The Precise HEPA-Filtered Glove Boxes offer many unique features to 

enhance safety and performance. To take full advantage of them, please 

acquaint yourself with this manual and keep it handy for future reference. 

All glove box operators must read and understand how HEPA filtered glove 

boxes operate; please review Chapter 4: Performance Features and Safety 

Precautions before beginning to work with this glove box. Even if you are 

an experienced user, please review Chapter 5: Using Your HEPA-Filtered 

Glove Box, which describes the Precise features so that you can use the 

filtered glove box effectively and safely. 
2 Product Service 1-800-522-7658 

Chapter 2:

Prerequisites

Before you install the glove box, you need to prepare your site for 

installation. You must be certain that the area is level and of solid 

construction. In addition, a dedicated source of electrical power should be 

located near the installation site to power the glove box. The glove box 

should be strategically placed in the lab to provide efficient workflow and 

prevent operator interference from normal traffic patterns. 

Carefully read this chapter to learn the requirements for your installation site: 

• The support, vibration and preventive requirements. 

• The location requirements. 

• The exhaust and blower requirements. 

• The electrical power requirements. 

• The space requirements. 

Refer to Appendix B: Precise HEPA-Filtered Glove Box Dimensions for 

complete glove box dimensions. 

Refer to Appendix C: Precise HEPA-Filtered Glove Box Specifications for 

complete electrical and environmental conditions, specifications and 

requirements. 
Chapter 2: Prerequisites

Product Service 1-800-522-7658 3

Support, Vibration and Preventive Requirements 

In the preparation of a glove box site, please consider the following: 

• A bench or stand that is rigidly mounted to the floor or fixed to the wall, 

but not both, may be appropriate. 35" to 40" (889mm-1016mm) is 

typical for standing height. (Labconco stands offered in Chapter 7 vary 

from 33" to 40".) 

• The corners of a building typically have less vibration than the center, 

which promotes analytical balance stability. 

• The bench typically should not contain any vibration-producing 

equipment, such as shakers or pumps. 

• A marble slab with dampening pads placed within the enclosure is an 

effective low cost means of controlling vibration (see Chapter 7: 

Accessorizing Your Filtered Glove Box). 

Location Requirements 

The Precise HEPA-Filtered Glove Boxes have been designed to rest on a 

typical 29"-30" (737mm-762mm) deep work surface. The height should be 

35"-40" for standing position. Avoid placing the glove box in high traffic 

areas where walking might disrupt the operator or experimentation. 

Exhaust and Blower Requirements 

The Precise HEPA-Filtered Glove Box, as manufactured, uses an integral 

motorized impeller to pull room air through the inlet and exhaust HEPA 

filters and discharge the clean air back into the laboratory. This negative 

pressure operation mode pulls all internally-contaminated air through the 

exhaust HEPA filter. The HEPA-filtered exhaust air is then forced out the 

top of the glove box. An optional FilterMateTM Portable Exhauster with a 

carbon filter may be installed downstream of the exhaust to adsorb low levels 

of organic fumes, formaldehyde, or ammonia gases. 

The HEPA-filtered exhaust air from the glove box can be exhausted to the 

outside with the installation of a thimble exhaust connection kit and remote 

blower listed in Chapter 7. As an option, if necessary, the built-in motorized 

impeller can be wall mounted if desired. 

Labconco does not recommend a hard duct connection using the built-in 

motorized impeller as the source of airflow out of the facility. This 

creates a positive pressure in the exhaust duct. 
Chapter 2: Prerequisites

4 Product Service 1-800-522-7658 

Labconco does not recommend hard duct connections with remote 

blowers due to the air volume balance requirements with such systems. 

Hard duct connections should only be used when the glove box impeller 

is disconnected and only a single roof-mounted blower is used. Note that 

reliance on an external exhaust blower does not provide airflow control 

at the glove box. 

If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1) 

is desired, the motorized impeller can be rotated to work in positive pressure 

mode (see Figure 3-3). See Chapter 7 for the Positive Pressure Conversion 

Kit. See the following Negative and Positive Pressure Particle Test data in 

the charts below. Both charts show the dilution rates of the glove boxes. 

Precise HEPA-Filtered Glove Box Particle Testing under Negative Pressure

0

24

14 13 11 12 11 11 9 6 4 0

0

60

0 1 1 1 1 000 000 0

50

100

150

200

250

300

350

400

450

500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Time (Minutes)

Number of Particles (Per Cubic Meter Per Minute)

> 0.3 Micron

> 0.5 Micron

Airflow Testing Performed at 50 Dial Setting (61 CFM) 

with Glove Box Under Negative Pressure. 

NOTE: Ambient Air Baseline Particle Count at 1 Minute

294132 Particles measuring 0.3 Micron detected within 1 Minute.

33296 Particles measuring 0.5 Micron detected within 1 Minute.
Chapter 2: Prerequisites

Product Service 1-800-522-7658 5

Precise HEPA-Filtered Glove Box Particle Testing under Positive Pressure

0

139

18 8 0

0

306

12 0 2 1 0

50

100

150

200

250

300

350

400

450

500

0123456

Time (Minutes)

Number of Particles (Per Cubic Meter Per Minute)

> 0.3 Micron

> 0.5 Micron

Airflow Testing Performed at 50 Dial Setting (61 CFM) 

with Glove Box Under Positive Pressure. 

NOTE: Ambient Air Baseline Particle Count at 1 Minute

198671 Particles measuring 0.3 Micron detected within 1 Minute.

25473 Particles measuring 0.5 Micron detected within 1 Minute.

ISO Class 3 Definition 

Airborne particulate cleanliness inside any clean air glove box or enclosure is 

designated by ISO Class 3, which is equivalent to 35 particles 0.5 μm or 

larger per cubic meter of air per minute as defined by ISO Standard 14644-1. 

ISO Class 3 cleanliness is illustrated in the table below and is equivalent to 

Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5 

μm or larger per cubic foot of air per minute. 

Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones. 

ISO 

classification 

number (N) 

Maximum concentration limits (particles/m3 of air/minute) for particles equal to 

and larger than the considered sizes shown below (concentration limits are 

calculated in accordance with 3.2 of Standard 14644-1) 

0.1 μm 0.2 μm 0.3 μm 0.5 μm 1 μm 5 μm 

ISO Class 1 10 2 

ISO Class 2 100 24 10 4 

ISO Class 3 1 000 237 102 35 8 

ISO Class 4 10 000 2 370 1 020 352 83 

ISO Class 5 100 000 23 700 10 200 3 520 832 29 

ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293 

ISO Class 7 352 000 83 200 2 930 

ISO Class 8 3 520 000 832 000 29 300 

ISO Class 9 35 200 000 8 320 000 293 000 

Table 2-1 ISO Classification Number (N) 
Chapter 2: Prerequisites

6 Product Service 1-800-522-7658 

Additionally, the motorized impeller can also be used in a full recirculation 

mode providing the operator with some control of the atmosphere chemistry 

within the glove box. See Chapter 7 for the Recirculation Conversion Kit. 

The airflow chart below represents typical operational data including air 

changes per minute, exhaust volume, noise levels, main chamber static 

pressures, and total system static pressures measured at the blower intake 

(suction side). Exhaust volumes are reduced by 17% for products operating 

on 100V, 60 Hz and reduced 34% for 100V, 50 Hz products. 

Dial Setting 

Air Changes 

per Minute 

(acm) 

Exhaust 

Volume 

(CFM) 

Noise Pressure 

dB (A) 

Static Pressure 

Inside Glove Box 

(inches of water) 

Total System 

Static Pressure 

(inches of water) 

Min. Dial 

20 Dial 

40 Dial 

50 Dial 

60 Dial 

80 Dial 

Max. 100 Dial 

Dial OFF 

1 

1.5 

3.3 

4.7 

6.2 

7.2 

7.7 

0 

13 

20 

43 

61 

80 

93 

100 

0 

44 

48 

48 

65 

68 

70 

71 

37 

.14 

.17 

.45 

.68 

.80 

.89 

.91 

0 

.34 

.51 

1.32 

1.81 

2.14 

2.38 

2.42 

0 

PRECISE HEPA-FILTERED GLOVE BOX

Static Pressure Test Results

0.14 0.17

0.45

0.68

0.8

0.89 0.91

0.3435

0.5072

1.323

1.81

2.141

2.375 2.422

0

0.5

1

1.5

2

2.5

10 20 30 40 50 60 70 80 90 100

Airflow Volume (Cubic Feet per Minute)

Negative Static Pressure 

(Inches of Water Column)

Static Pressure Inside Glove Box

Total Static Pressure at Blower

Intake (Suction Side)
Chapter 2: Prerequisites

Product Service 1-800-522-7658 7

For thimble-ducted glove boxes, the proper remote blower selection can be 

determined from these exhaust requirements as the remote blower only needs 

to be sized for the static pressure loss of the ductwork system. The thimble 

ducted exhaust blower should be sized for 25% additional airflow. When 

exhausting a glove box to the outside, it should be connected to a dedicated 

remote blower. 

Labconco offers accessory remote blowers listed in Chapter 7. Contact 

Labconco for blower sizing assistance. 

When a glove box is connected to a remote blower exhaust system, an 

adjustable damper (or valve) should be installed in the duct to control the 

airflow volumes properly. See Chapter 7 for Exhaust Dampers. 

Electrical Requirements 

Electrical service should be nearby for connecting the glove box, and 

accessory equipment. The glove box is rated 115V, 8A or 230V, 4A or 

100V, 8A. A convenience duplex outlet is located inside the glove box and is 

rated for 115V, 5A or 230V, 2A or 100V, 5A. Additional power strip 

accessories are available for connection to the duplex outlet and are listed in 

Chapter 7. 

Space Requirements 

The dimensions are shown in Appendix B: Dimensions. 

! 
8 Product Service 1-800-522-7658 

Chapter 3:

Getting Started

Once the site for your Precise Glove Box is properly prepared, you are ready 

to unpack, inspect, install, and validate your system. Read this chapter to 

learn how to: 

• Unpack and move the glove box. 

• Set up the glove box with the proper supporting structure and work 

surface. 

• Connect to an exhaust system if applicable. 

• Install the gloves. 

• Connect the electrical supply. 

• Set the exhaust volume with the speed control adjustment. 

• Arrange validation for the glove box. 

Each Precise Glove Box weighs 205 lbs. (90 kg). The 

shipping container allows for lifting with a mechanical lift 

truck or floor jack. If you must lift the enclosure manually, 

follow safe-lifting guidelines. Do not lift by the upper sheet 

metal façade and front panel as damage can occur to the 

plastic glove box liner (see Figure 3-1).

! 
Chapter 3: Getting Started

Product Service 1-800-522-7658 9

Figure 3-1 

Lifting Instructions 

Unpacking the Glove Box 

We recommend that you do not remove the glove box from its shipping 

container until it is ready to be placed into its final location. Move the unit 

by placing a flat, low dolly under the shipping skid, or by using a floor jack. 

Carefully remove the carton shrink-wrap on the glove box and inspect it for 

damage that may have occurred in transit. If damaged, notify the delivery 

carrier immediately and retain the entire shipment intact for inspection 

by the carrier. 

THE UNITED STATES INTERSTATE COMMERCE 

COMMISSION RULES REQUIRE THAT CLAIMS BE 

FILED WITH THE DELIVERY CARRIER WITHIN 

FIFTEEN (15) DAYS OF DELIVERY. 

DO NOT RETURN GOODS WITHOUT THE PRIOR 

AUTHORIZATION OF LABCONCO. UNAUTHORIZED 

RETURNS WILL NOT BE ACCEPTED. 

)

)
Chapter 3: Getting Started

10 Product Service 1-800-522-7658 

IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU 

MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT 

CARRIER. LABCONCO CORPORATION AND ITS 

DEALERS ARE NOT RESPONSIBLE FOR SHIPPING 

DAMAGES. 

Do not discard the packing material until you have checked all of the 

components and tested the glove box. 

Installing the Glove Box on a Supporting 

Structure and Work Surface 

Exercise caution when lifting or moving the glove box. 

When installing the glove box onto a work surface or benchtop, ensure that 

the structure can safely support the combined weight of the glove box and 

any related equipment. The work surface should be as wide as the glove box 

to properly support it. The front of the glove box should be aligned with the 

front of the work surface for optimal comfort. A height of 35"-40" (889mm1016mm) is appropriate for standing operation. Adjustable Height Base 

Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm). 

Connecting to the Exhaust System (If Applicable) 

WARNING: The weight of any exhaust ductwork system 

must be supported independently of the glove box 

superstructure or damage may occur. 

The exhaust system should be installed by a qualified 

HVAC contractor. 

The Precise HEPA-Filtered Glove Box has been manufactured to exhaust 

into the laboratory in its standard configuration. To remove chemical fumes 

and odors not trapped by the HEPA filter, the glove box can be exhausted to 

the outside, or connected to an accessory FilterMate Portable Exhauster with 

carbon filters (see Figure 3-2). See Chapter 7 for accessories including: 

Thimble Exhaust Connection, FilterMate Portable Exhauster, Positive 

Pressure Conversion Kit, Recirucation Kit, Hard Duct Connection. 

Consult Labconco Customer Service if you require help sizing a remote 

blower for the exhaust volume and the duct work system (static pressure loss 

listed in Chapter 2). 

To ensure compatibility, the selected exhaust duct material 

should match the enclosure, procedures and chemical 

applications. 

)

! 

! 
Chapter 3: Getting Started

Product Service 1-800-522-7658 11

Figure 3-2 

Exhaust Connection to Outside 

(All dimensions in inches) 
Chapter 3: Getting Started

12 Product Service 1-800-522-7658 

Figure 3-3 

Positive Pressure Glove Box Configuration 

Installation of Gloves to the Glove Ports 

With thumbs up and right/left orientation, secure the gloves in place on the 

glove ports by stretching the beaded cuff into the groove nearest the window. 

Install the separate 8" diameter O-ring over the gloves and into the outer 

groove of the glove port surface. Stainless steel band clamps are provided for 

securing the separate O-ring into the glove port groove. Replacement gloves 

and parts are listed in Appendix A with a description in Chapter 7. 

Figure 3-4 

Glove Installation 
Chapter 3: Getting Started

Product Service 1-800-522-7658 13

Connecting the Electrical Supply to the Glove Box 

115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models 

Connect the power cord supplied to the IEC electrical supply plug on the 

back of the glove box. 115V or 100V models are rated at 8 amps total. 

The maximum circuit load for the interior electrical duplex is 5 amps. 

230V, 50 Hz or 230V, 60 Hz Models 

The same procedure applies for the 230V model except it is shipped without 

a plug. Install the appropriate plug per local requirements. The 230V, 50 Hz 

or 230V, 60 Hz models are both rated at 4 amps total. The interior 

electrical duplex is rated at 2 amps. 

Setting the Exhaust Volume with the Speed 

Control Adjustment 

For Precise HEPA-Filtered Glove Boxes, adjustment of the speed control 

provides control of exhaust air volume and is located on the front panel. The 

exhaust air volume should be adjusted at the low setting for weighing 

operations. (Consult your Safety Officer for airflow recommendations 

for your application). Working at the lowest exhaust volume appropriate 

for the application will give the quietest operation. To increase the rate of air 

volume changes after working in the glove box and to ensure proper cleaning, 

the speed control should be set at a higher (50 to 100) dial setting for 5 to 10 

minutes as recommended by the operator’s predetermined protocol and the 

chart below. The exhaust volume is increased by turning the speed control 

counterclockwise. 

Dial Setting 

for Cleaning 

Internal Air 

Volume 

Approximate 

Air Volume 

Changes per 

Minute 

Estimated 

Total Exhaust 

Volume 

(CFM) 

Minimum 

Recommended 

Times to 

Clean Internal 

Air Volume 

(minutes) 

50 4.7 61 10 

100 7.7 100 5 
Chapter 3: Getting Started

14 Product Service 1-800-522-7658 

Validating the Glove Box 

The Precise HEPA-Filtered Glove Box has been leak tested at the factory for 

HEPA filter and main chamber pressure integrity. The HEPA filters should 

be leak checked upon start-up and annually thereafter per the procedure in 

Chapter 6. Upon start up, the main chamber pressure should be greater 

than 0.7" w.g. at full blower speed to validate proper blower operation; 

if not, contact Labconco for service.

NOTE: Main chamber pressure and smoke removal tests 

should be performed frequently, per your established 

quality system, to ensure safe performance. 

Summary of Performance Testing Results 

Labconco’s test results show that the Precise HEPA-Filtered Glove Box 

meets all the performance criteria when operated at the following estimated 

airflow volumes: 61 CFM or less for weighing with an analytical balance or 

handling powders and 61 CFM or above for safe air clean up. The Precise 

HEPA-Filtered Glove Box has been tested to the point of insufficient 

performance. In each case, the lack of performance occurred at airflow 

volumes below Labconco’s minimum recommended airflow volume for the 

specific operation. Contact Labconco or visit www.labconco.com for 

complete performance test data. The following provides a brief summary of 

the findings: 

• Smoke removal was tested at minimum 13-CFM to maximum 100-CFM 

airflow volumes. The time required to completely purge the main 

chamber of smoke varied from approximately 75 seconds at 100-CFM, to 

420 seconds at 13-CFM. 

• During tracer gas containment tests with the glove box vented to the 

outside, no gas was detected within the operator’s breathing zone. 

Perimeter scans were also conducted during tracer gas testing of the 

Precise HEPA-Filtered Glove Box. Under normal laboratory conditions 

the tracer gas levels detected during perimeter scan testing were below 

ASHRAE 110 permissible limits (0.10 PPM average or less) and 

achieved Labconco permissible limits (0.05 PPM average or less). 

• Sound levels “Noise Pressure” varied from 44 dB(A) at 13-CFM to 71 

dB(A) at 100 CFM. If vented to the outside, the sound level will be 

greatly reduced. 

• Airborne particulate cleanliness (measured under positive and negative 

pressures) within the main chamber of the glove box exceeded ISO Class 

5 conditions (equivalent to Class 100) and achieved ISO Class 3 

conditions (equivalent to Class 1) at both minimum and maximum 

operational airflow volumes. 

• Glove box fluorescent lamp light levels “illumination” (measured across 

the work surface) averaged 29.9 foot candles with room lights turned off 

! 
Chapter 3: Getting Started

Product Service 1-800-522-7658 15

and 33.7 foot candles with the test lab’s overhead fluorescent lights 

turned on. 

• Static pressure measured inside the glove box varied from negative 0.14 

inch of water column at minimum 13-CFM to negative 0.91 inch of water 

at maximum 100-CFM. The total system static pressure measured 

between the exhaust filter and the blower varied from negative 0.34 inch 

of water column at 13-CFM to negative 2.4 inches of water at 100-CFM. 

• The vibration level “displacement” measured on the work surface is 

negligible at minimum through maximum airflow volume settings. The 

measured level of displacement ranged from 0.59 x 10-5 inches to 1.45 x 

10-5 inches. 

• The static electricity measured on the work surface and interior surfaces 

of the glove box achieved a negligible level (below +/- 100V on any 

surface) with the glove box blower operating at a min 13-CFM to max 

100-CFM at 22% relative humidity. Test conditions included the ionizer 

fan speed set to high output and the potentiometer within the ionizer was 

adjusted slightly between positive and negative output in order to 

maintain balanced ionization. The time required for sufficient 

electrostatic decay will vary depending upon user application, moisture 

levels within the supply air and the level of cleanliness within the glove 

box interior. Anti-Static Ionizer Fan is sold separately (see Chapter 7). 

• The helium leaks ranged from 2 x 10-4 to 1 x 10-6 ml/sec with no 

individual leak greater than the specified 1 x 10-3 ml/sec. 

• Balance stability testing results were excellent with no detectable 

instability of an analytical balance to five decimal places and a top loader 

balance to three decimal places. Test results were confirmed for various 

sample weights with the glove box running at various blower airflow 

settings. 
16 Product Service 1-800-522-7658 

Chapter 4:

Performance Features and

Safety Precautions

Performance Features 

The Precise HEPA-Filtered Glove Box is designed to meet the needs of the 

laboratory scientist, and provide superior particulate containment. The 

HEPA-filtered glove boxes have been tested to effectively contain toxic and 

noxious materials when properly installed and operated. Labconco 

engineered the HEPA-Filtered glove boxes to minimize the effects of 

turbulence. The concentrations of particulate materials are predominantly 

removed from the main chamber by controlling the frequency of main 

chamber air volume changes. See Figure 4-1 to refer to a detailed description 

of the main performance features. 

1. Molded One-Piece Chemical-Resistant Glove Box Shell made of 

medium density polyethylene, which provides superior chemical 

resistance. The molded liner is seamless and easy to clean with coved 

interior corners. 

2. Versatile Vibration Isolated Motorized Impeller Isolation supports 

eliminate the transfer of vibration to the work surface which is extremely 

important in weighing operations with precision analytical and micro 

balances. The impeller wheel is also dynamically balanced capable of a 

minimum airflow of 13 CFM to a maximum of 100 CFM. Measured 

displacement for vibration is less than 1.5 x 10-5 inches. Additional 

unique features of the impeller include an optional Positive Pressure Kit, 

a Recirculation Kit, and a Blower Wall Mount Kit (see Chapter 7). 

3. HEPA Filter System consists of the inlet and outlet HEPA filters rated 

99.99% efficient on 0.3 micron particulates. Inlet and outlet HEPA filters 

are identical and include a test port for challenging the HEPA filter 

integrity. An optional filter bag may be ordered for bag-in/bag-out filter 

change procedures (see Chapter 7). 
Chapter 4: Performance Features and Safety Precautions

Product Service 1-800-522-7658 17

4. Main Chamber Static Pressure Gauge provides an indication of HEPA 

filter condition inside the glove box. Typically, HEPA filters should be 

replaced when the static pressure gauge reading has increased two-fold. 

The gauge can be changed to positive pressure for clean air glove box 

operations by reversing the hose on the gauge to the positive pressure 

connection. Positive Pressure Conversion Kit is sold separately (see 

Chapter 7). 

5. Glove Ports are 8" nominal size and molded into the main glove box 

shell. The double grooves provide two points of restraint for safe 

changing of the gloves. 

6. Neoprene Gloves are supplied with the glove box, .015" thick, 30" long, 

one pair, size 9 3⁄4. See Appendix A for replacement parts and Chapter 7 

for a description. 

7. Removable Framed Glass Window is 1/4" thick laminated safety glass 

and is reinforced with powder-coated steel frame. The removable glass 

window frame provides access to the interior for loading large apparatus. 

When removed, the window opening is 26.4" wide x 12.5" high (670mm 

wide x 317mm high). The framed glass window is sealed with a onepiece molded neoprene gasket. See Chapter 7 for optional Acrylic 

Viewing Window with Frame. 

8. Fluorescent Lamp provides 30-40 foot candles of interior illumination 

on the work surface. The lamp may be replaced from outside the glove 

box. See Chapters 6 and 7 for replacement. 

9. Control Panel consists of the main chamber static pressure gauge, 

switches for the light, blower, duplex outlet, and speed control. The 

speed control can vary the impeller speed from 13 to 100 CFM. 

10. Work Surface is black chemically-resistant phenolic core and sealed to 

the glove box floor. The black surface aids proper cleaning and provides 

high visibility of powders. Phenolic core has superior chemical resistance 

and stain resistance. 

11. Interior Switched Duplex is located on the glove box ceiling. The 

innermost outlet of the duplex labeled “AUX” is switch operated on the 

front control panel. An accessory Electrical Power Strip can be 

connected and ordered from Chapter 7. The interior duplex is rated at 

115V, 5A or 230V, 2A or 100V, 5A.

12. Transfer Chamber Doors have a molded one-piece neoprene gasket 

with a quick operating latch for user comfort. The counterweighted doors 

swing up to save space. IMPORTANT: Transfer chamber must have 

an optional High Vacuum Sleeve if the chamber is converted for 

vacuum operation. See Chapter 7.
Chapter 4: Performance Features and Safety Precautions

18 Product Service 1-800-522-7658 

 Figure 4-1 
Chapter 4: Performance Features and Safety Precautions

Product Service 1-800-522-7658 19

Safety Precautions 

1. It is the responsibility of the user to determine the suitability of this 

product for the intended applications. Consult your Safety Officer. 

2. Prior to using the glove box, check to make sure that the exhaust 

blower is operating and that air is entering the glove box by observing 

the static pressure gauge. 

3. Use good housekeeping in the enclosure at all times. Clean up spills 

immediately. Periodically clean glove box interior. 

4. This product is not designed or intended to be explosion proof. It is 

the responsibility of the user to determine and avoid lower explosive 

limits and flammability of any enclosed chemicals or gases. The user 

is also responsible for taking proper precautions to prevent equipment 

damage or injury due to explosion or combustion. 

5. Avoid the use of flammable gases or solvents in the glove box. Care 

must be taken to ensure against the concentration of flammable or 

explosive gases or vapors. Use of an open flame or exposure to high 

heat should be avoided in the glove box as heat can damage the liner. 

Heat may harm the HEPA filter and damage the filter’s adhesive. 

6. Perchloric acid use in this enclosure is prohibited. 

7. Radioisotope usage in the glove box should be cleared with your 

Safety Officer. 

8. A qualified certification technician should test the integrity of the 

HEPA filters before the box is initially used. 

9. The glove box should be recertified whenever it is relocated or 

serviced and at least annually thereafter. 

10. The use of safety goggles, protective clothing, and any other personal 

protective equipment recommended by your safety officer should be 

employed. 

11. The HEPA filter provides personnel and environmental protection 

from particulate matter. Should the blower be reversed to positive 

pressure inside, this glove box may be used for operations requiring 

product protection from environmental particulate contamination. 

12. HEPA filters are only effective for entrapment of particulate matter. 

Manipulations that generate gases or vapors from toxic chemicals or 

radionuclides are not captured by the HEPA filters. 
Chapter 4: Performance Features and Safety Precautions

20 Product Service 1-800-522-7658 

13. The surface of the HEPA filter is fragile and should not be touched. 

Care must be taken to avoid puncturing the HEPA filter during 

installation or normal operation. If you suspect that a HEPA filter has 

been damaged DO NOT use the glove box; contact a local 

certification agency or Safety Officer. 

14. The HEPA filters in the glove box will gradually accumulate airborne 

particulate matter from the room and from work performed in the 

glove box. The rate of accumulation will depend upon the cleanliness 

of the room air, the amount of time the glove box is operating and the 

nature of work being done in the glove box. With normal usage, the 

HEPA filters will last two to five years before requiring replacement 

(see Chapter 6). 

15. Ensure that the filtered glove box is connected to an electrical service 

in accordance with local and national electrical codes. Failure to do 

so may create a fire or electrical hazard. Do not remove or service 

any electrical components without first disconnecting the glove box 

from electrical service. 

16. Used HEPA filters may be hazardous waste. The user is responsible 

for recording chemicals used and disposal of used filters. 

17. Ensure only trained operators use the glove box. New users must

review the User’s Manual with emphasis on Routine Daily Work 

Procedures in Chapter 5 and become familiar with the safe operation 

of the glove box. 

18. Only powders and particulates removed by HEPA filters are 

appropriate for use in this glove box. Vapors and other gases should 

be exhausted to the outside or adsorbed with the use of an accessory 

FilterMate Portable Exhauster equipped with carbonfilters. See 

Chapter 7. 

19. Leave the impeller on for at least 5-10 minutes at 50%-100% speed 

after work in the glove box has been completed to purge the interior 

of containments. 

20. If a chemical, powder or particulates are spilled on the work surface, 

DO NOT switch off the blower until all traces have been removed. 

21. Tag glove box with appropriate warning if filters have been removed 

or the glove box requires servicing. 

22. If the blower fails during use, processes should cease and the area 

should be vacated. Inform your Safety Officer immediately. 

23. Consult your Safety Officer before removing any hoses to the static 

pressure gauge or exhaust hoses as they may be contaminated. 
Product Service 1-800-522-7658 21

Chapter 5:

Using Your Glove Box

Now that the installation of your glove box is completed, you are ready to use 

your Precise HEPA-Filtered Glove Box. 

Read this chapter to learn about: 

• Routine Daily Work Procedures 

• Appropriate HEPA Filter Applications, Suitability and Guidelines 

• Carbon Filter Exhaust Applications 

• Definition of Terms 

• Appropriate Chemicals for Carbon Filters 

• Hazardous Misapplications for Carbon Filters 

• Chemical Carcinogen use with Carbon Filters 

• Acid Use 

Routine Daily Work Procedures 

Planning

• Thoroughly understand procedures and equipment required before 

beginning work. 

Start-up

• Consult your Safety Officer for personal protective equipment 

recommendations. 

• Turn on exhaust system and light. 

• Allow the enclosure to operate for 2-5 minutes at 50-100% blower 

speed. 
Chapter 5: Using Your Glove Box

22 Product Service 1-800-522-7658 

Loading Materials and Equipment (see Figure 5-1)

• Load only the materials required for the procedure. Do not overload 

the glove box. 

• Be aware that gloves will inflate when the glove box is operated 

under negative pressure. Ensure items in the glove box will not 

obstruct the movement of the gloves. 

• After loading, wait 2-5 minutes at 50-100% blower speed to purge 

airborne contaminants from the work area before beginning 

procedures. 

Work Techniques

• Segregate all clean and contaminated materials in the work area. 

Final Purging (see Figure 5-1)

• All open trays, weigh vessels or containers must be sealed before 

being removed from the glove box. 

• Objects in contact with contaminated material must be surface 

decontaminated before removal from the glove box. 

• The interior surfaces should be cleaned per user’s protocol. 

• Upon completion of work, the glove box should be allowed to operate 

for 5 minutes undisturbed at 100% speed or 10 minutes at 50% speed 

to purge and dilute airborne contaminants from the work area. 

• After purging the main chamber, the transfer chamber should also be 

purged for 5 minutes at 100% speed or 10 minutes at 50% speed with 

the inner door open, before removing sealed material. 

Shutdown

• Turn off the exhaust system and light. 

• Keep outer door closed when not in use. 
Chapter 5: Using Your Glove Box

Product Service 1-800-522-7658 23

Figure 5-1 

Loading and Final Purging Technique 

HEPA Filter Applications, Suitability and 

Guidelines 

• Microbiological materials and hazardous solids containment protecting 

only the operator and the surrounding environment. 

• Weighing of powders. 

• Containment of hazardous particulates using a HEPA filter. 

• Powder or particulate procedures traditionally performed on an open 

bench. 

• The HEPA filtered glove box provides personal and environmental 

protection from particulate matter, hazardous powders and microbiological 

materials. 

• This glove box can be used for sample protection by reversing the 

blower airflow direction, causing the main chamber to be under positive 

pressure. Positive Pressure Conversion Kit is required (see Chapter 7). 

• Manipulations that generate gases or vapors, i.e., toxic chemicals or 

radionuclides, require the use of a HEPA and carbon filter in 

combination. Otherwise the glove box should be ducted to the outside. 

• Each filtered glove box is provided with two HEPA filters. Follow the 

HEPA Filter change procedure in Chapter 6 for disposal instructions. 

For microbiological materials, follow the Decontamination Procedure in 

Chapter 6 for disposal instructions. 

Safety Note: Must seal and wipe down all 

chemical containers.
Chapter 5: Using Your Glove Box

24 Product Service 1-800-522-7658 

Definition of Terms 

NIOSH – National Institute for Occupational Safety and Health/Mine Safety 

and Health Administration. (U.S.A.) 

TWA – Recommended Exposure Limits expressed as a Time Weighted 

Average. The exposure limit for that chemical for up to a 10-hour workday, 

40 hours a week. Expressed in units of parts per million or milligrams per 

cubic meter. 

Odor Threshold – The value in parts per million or milligrams per cubic 

meter for which one might expect to smell a chemical’s presence in the air. 

This value is very subjective and detection will vary with the sensitivity of 

one’s nose. The period of time until the odor threshold is reached in the 

exhaust stream can be estimated from Labconco exclusive computerized filter 

modeling program. Contact Labconco on carbon filter life for specific 

applications. See Chapter 6. 

Saturation Level or Time – There is a limit to the amount of chemical that 

can be adsorbed by activated carbon, or neutralized by chemically-treated 

carbon. Once the capacity of the carbon is reached, it is considered to be 

saturated and will adsorb (or neutralize) no further material; the outlet 

concentration of the chemical will equal the inlet concentration from that 

point until the filter is replaced. (Note that the capacity of activated carbon is 

not a constant, but varies with the inlet concentration). Labconco Technical 

Specialists can determine with the computerized carbon-modeling program 

the estimated time saturation for a particular chemical. When using a HEPA 

filter in all filtered glove boxes or in combination with a carbon filter, the 

speed control will need to be increased to allow for HEPA filter loading. 

IDLH (Immediately Dangerous to Life and Health). An atmosphere that 

poses an immediate hazard to life or produces immediate irreversible health 

effects. IDLH concentrations should not be approached in the glove box. 

Appropriate Chemicals for Carbon Filters 

Below is a general set of rules to determine appropriateness of chemical 

usage. 

Selected organic chemicals considered to be occupational 

carcinogens by NIOSH can be used in the filtered glove 

box with carbon filters under rigid restrictions. See 

separate discussion on carcinogens for special instructions. 

Organics must have time weighted exposure limits (TWA) of 1 PPM or 

greater. 

Chemicals must have a detectable odor at concentrations below the TWA for 

the chemical. 

! 
Chapter 5: Using Your Glove Box

Product Service 1-800-522-7658 25

Chemicals must be designated by NIOSH guidelines as acceptable for use 

with chemical cartridge-type respirators (the exception is formaldehyde and 

ammonia/amines, which use impregnated carbon). Chemicals not listed by 

NIOSH in the Pocket Guide must be approved by Labconco Product 

Specialist (or Engineering). 

Inlet concentration must never exceed the IDLH (Immediately Dangerous to 

Life and Health) concentrations. 

Chemicals having a recommendation by NIOSH of at least “Escape 

GMFOV” (Gas Mask Full-Face Respirator). 

When evaporating a mixture of chemicals, the chemical having the lowest 

TWA will be used to determine if the mixture meets the guidelines. 

Call a Labconco Product Specialist at 1-800-821-5525 for assistance in 

chemical appropriateness. 

Hazardous Misapplications for Carbon Filters 

with Volatile Chemicals 

There is one scenario where the accessory FilterMate carbon filter 

misapplication would be a part of a hazardous condition. If the user 

continues to operate the glove box with FilterMate Portable Exhauster with 

any of the following conditions, a potentially hazardous condition will exist: 

1. The inlet concentration of vapors is greater than the TWA. 

2. The carbon filter becomes saturated. 

3. The ventilation of the room is insufficient to dilute the exhaust of the 

enclosure to below the TWA for the chemical. 

When the inlet concentration is greater than the TWA, extra measures must 

be taken to monitor the filter and number of room air exchanges. 

Chemical Carcinogen Use with Carbon Filters 

Selected carcinogens may be used safely with accessory FilterMate carbon 

filters under the following restrictions. 

The use of a ventilated glove box with ducting to the 

outside is always the preferred method when working with 

carcinogens. The carbon filters should only be used, as a 

last resort, when venting to the outside is not an option.

The potential carcinogens are listed in the NIOSH Pocket Guide to Chemical 

Hazards as “Ca.” Each potential carcinogen must have a TWA of 1 or 

greater; have minimum respirator recommendation of Escape GMFOV, and 

an odor threshold significantly lower than the TWA for the chemical. 

! 
Chapter 5: Using Your Glove Box

26 Product Service 1-800-522-7658 

The inlet concentration or the evaporation rate of the chemical must never 

exceed the TWA for the chemical. 

Consult a Labconco Technical Specialist for estimated saturation life. See 

Chapter 6 for an example of estimating saturation life based on Labconco’s 

chemical guide. 

Prohibited Acid Use 

The Precise HEPA-Filtered Glove Box and internal metal parts must not be 

exposed to mineral acid use. No exceptions are permitted, as the glove box 

life span will be limited with acid use. 

NOTE: If mineral acids are used with the Precise HEPA-Filtered Glove 

Box, Labconco makes no claims about life span and warranties 

will become void.
Product Service 1-800-522-7658 27

Chapter 6:

Maintaining Your Glove Box

Review this chapter on maintenance for the following: 

1. Routine maintenance. 

2. Decontamination. 

3. Determining when to replace the HEPA filters. 

4. How to install new HEPA filters. 

5. HEPA filter leak test. 

6. Initial certification. 

7. Re-certification. 

8. Fluorescent light replacement. 

9. Motorized impeller replacement. 

10. Speed control replacement. 
Chapter 6: Maintaining Your Glove Box

28 Product Service 1-800-522-7658 

Routine Maintenance Schedule 

Weekly

• Wipe down the interior surfaces of the glove box with a disinfectant 

or cleaner, depending upon the application. 

• Using a damp cloth, clean the exterior surfaces of the glove box, 

particularly the front and top, to remove any accumulated dust. 

• Operate the exhaust system, noting the airflow through the glove box 

to effectively remove a source of visible smoke. 

Monthly (or more often as required)

• Chart the operating static pressure at a typical speed control setting. 

Change HEPA filters when the static pressure increases two-fold or 

speed setting increases two-fold. GuardianTM Jr. Airflow Monitors are 

available for constant monitoring (see Chapter 7). 

• While the glove box is filled with the contaminant gas, test filter 

condition of accessory FilterMate carbon filters using the appropriate 

gas detector tube at intervals of 20% of the total estimated time. The 

exception to the 20% recommendation is formaldehyde or any 

carcinogen or suspected carcinogen. These hazardous chemicals must 

be checked at least every 10% of the total estimated time. Gas 

detector tubes for the specific chemicals that are being used in the 

glove box can be obtained from your laboratory supply dealer (refer 

to FilterMate manual). 

• Replace accessory FilterMate carbon filters when chemical 

breakthrough is indicated by odor, time, detector tube, or for some 

chemicals, analytical instrumentation. 

Annually

• Replace the fluorescent lamps. 

• Have the glove box re-certified by a qualified certification technician. 

See Certification and Recertification in Chapter 6. 

Accessory 

FilterMate 

Carbon 

HEPA 

Accessory 

FilterMate 

Carbon 
Chapter 6: Maintaining Your Glove Box

Product Service 1-800-522-7658 29

Decontamination 

When used in conjunction with microbiologicals, the glove box should be 

decontaminated with formaldehyde gas before: 

• Performing maintenance work in contaminated areas 

• HEPA filter changes 

• Moving the glove box to a new location 

• Changing research programs 

The procedures for performing a gaseous decontamination are thoroughly 

outlined in the U.S. Department of Health, Education and Welfare booklet 

entitled Formaldehyde Decontamination of Laminar Flow Biological Safety 

Cabinets, available from NIH, Division of Safety, Bethesda, MD 20892, call 

301-496-2801. 

Determining When to Replace HEPA Filters 

The HEPA filters in the filtered glove boxes gradually accumulate airborne 

particulate matter and powders from the enclosure and room. The rate of 

accumulation will depend upon the cleanliness of the room air, the amount of 

time the enclosure is operating, and the nature of work being done in the 

enclosure. In typical installations and usage, the HEPA filters will last two to 

five years before requiring replacement. Replace HEPA filters if the speed 

control is adjusted to full speed or if the normal operating speed and/or static 

pressure is double the original starting point. Replace HEPA filters if it fails 

the HEPA Filter Leak Test in Chapter 6. 

How to Install New HEPA Filters 

The Precise HEPA-Filtered Glove Boxes include a true bag-in/bag-out filter 

disposal system for safely removing the HEPA filter and protecting the 

worker from toxic powders and particulates (see Figure 6-1, Filter Changing 

Diagram). The accessory bags can be ordered from Labconco (see Chapter 

7). Two bags will be required for each HEPA filter for a total of 4 each to 

complete the change process. For your safety, wear appropriate personal 

protective equipment during the change of HEPA filters. If working with 

micro-biologicals, use the decontamination procedure referenced above. 

(Consult your Safety Officer before performing filter changes). 

1. Turn the glove box OFF. Refer to Appendix A for replacement parts 

and Appendix B for dimensions. 

2. Loosen the six filter nuts that secure each HEPA filter. 

3. Place the accessory bag over the HEPA filter and seal to the filter 

housing. 
Chapter 6: Maintaining Your Glove Box

30 Product Service 1-800-522-7658 

4. Place both arms in the rear gloves of the bag, and carefully draw the 

HEPA filter out of the glove box. Rest the HEPA filter on the floor, 

table or cart. Remove arms from bag gloves. 

5. Create two seals between the glove box housing and the old HEPA 

filter. Seals can be heat sealed or sealed with tie wraps. Cut off the 

bag between the two seals and leave the bag stub on the access port to 

the glove box. 

6. Place the new HEPA filter in another new bag. Then place the new 

bag with the new HEPA filter over the old bag stub and onto the 

glove box filter housing. 

7. Carefully remove the old bag from the filter housing using the single 

glove near the access port. Draw the old bag and stub into the single 

glove and turn the glove inside out. Double seal this glove on the 

contaminated bag stub inside. Remove the bag and dispose of 

properly. 

8. Carefully install the new HEPA filter and uniformly tighten the six 

filter nuts until the HEPA filter gasket is compressed 50%. 

9. Turn the glove box ON and proceed to the HEPA Filter Leak Test in 

Chapter 6. 

Figure 6-1 

HEPA Filter Changing Diagram 
Chapter 6: Maintaining Your Glove Box

Product Service 1-800-522-7658 31

HEPA Filter Leak Test 

Purpose 

This test is performed to determine the integrity of the HEPA filter(s), the 

filter housing, and the filter mounting frames. The leak test is to be done by a 

qualified technician with calibrated equipment. Reference Leak Testing and 

Photometer scanning from the Institute of Environmental Services (IES-RPCC001.3) 

Equipment Required 

1. An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air 

Techniques Hamilton Associates inc. 11403 Cron Ridge Dr. Owings 

Mills, MD 21117 

2. One aerosol generator of the Laskin nozzle(s) type. An aerosol of 

mineral oil or suitable liquid shall be created by flowing air through it. 

The compressed air supplied to the generator should be adjusted to a 

pressure as indicated in the procedure. Air Techniques Inc. Model TDA4A or equal. 

3. Mineral oil (Labconco P/N 1491400). 

4. Sampling Nozzle, Rectangular 1/2" x 3-1/4", Air Techniques, Inc. 

Procedure 

When Using the ATI 2G Photometer 

NOTE: Because the downstream side of the HEPA filters cannot be 

scanned, a downstream average must be taken. 

1. Turn on the photometer and allow it to operate for a minimum of 5 

minutes. Leave the valve in the “CLEAR” setting. 

2. Press the “ENTER” keypad. Press the “REF” keypad. 

3. The display will show “P1” or “P2” for approximately 1 second, and 

then display a numerical value. 

4. Using the “▲” or “▼” keypads, increase or decrease the numerical 

value, respectively, until it equals 68. (Set for 100 CFM, adjust for 

other airflows.) 

5. Press the “ENTER” Keypad. The photometer will scan for 15 

seconds, and then the “0” keypad will flash. Press the “Enter” keypad. 

The unit will scan for 5 seconds, the display will read “0000,” and the 

unit will sound a confirming tone. 

6. Set the valve to “DOWNSTREAM.” Place the palm of your hand 

over the sampling port of the pistol. There should be a strong vacuum 

at this port. If the vacuum is weak, contact Air Techniques Hamilton 

Associates. 
Chapter 6: Maintaining Your Glove Box

32 Product Service 1-800-522-7658 

7. Ensure that the glove box speed control is adjusted to the maximum 

setting and the blower is exhausting 100 CFM through the glove box. 

8. Position the aerosol generator discharge near the internal opening of 

each HEPA filter. Check both filters. 

9. Start the aerosol generator. Ensure that the generator is operating 

with 1 nozzle on at 10 PSIG. 

10. Allow the generator to operate for a minimum of 15 seconds. Place a 

1/4" OD sampling tube in the exhaust downstream of the HEPA 

filters. The inlet HEPA filter can be checked from the test port on the 

exhaust HEPA filter and the exhaust HEPA filter can be checked from 

the blower inlet (suction side) test port. Connect this tube to the 

“downstream” sampling port of the photometer. 

Acceptance 

The downstream average penetration shall not exceed 0.005 percent as 

measured by the photometer. 

Procedure When Using the ATI 2-D or 2-E Photometer 

NOTE: Because the downstream side of the HEPA filters cannot be 

scanned, a downstream average must be taken. 

1. Turn on the photometer and allow it to operate for a minimum 5 

minutes. Leave the range switch at “100%” and the valve in the 

“CLEAR” setting. 

2. Use the screw on the face of the gauge to adjust the meter to 0 if 

necessary. 

3. If the “INT REF” switch is a square button, depress it. The switch will 

remain depressed and illuminated. If the switch is a toggle type 

switch, push and hold the switch to the “ON” position. Keep this 

switch in the “ON” position through step 4. 

4. Turn the “GAIN” knob until a value of 15 is obtained. (Set for 100 

CFM, adjust for other airflows.) 

5. Turn the range switch to 0.1. Turn the Stray Light knob until the 

meter reads “0.” 

6. Turn the valve to the “DOWNSTREAM” setting. Place the palm of 

your hand over the sampling port of the pistol. There should be a 

strong vacuum at this port. Return the valve to the “CLEAR” setting. 

7. Ensure that the glove box speed control is adjusted to the maximum 

setting and the blower is exhausting 100 CFM through the glove box. 

8. Position the aerosol generator discharge near the opening of each 

HEPA filter. Check both filters. 

9. Start the aerosol generator. Ensure that the generator is operating 

with 1 nozzle on at 10 PSIG. 

10. Allow the generator to operate for a minimum of 15 seconds. Place a 

1/4" OD sampling tube in the exhaust downstream of the HEPA 

filters. The inlet HEPA filter can be checked from the test port on the 

exhaust HEPA filter and the exhaust HEPA filter can be checked from 
Chapter 6: Maintaining Your Glove Box

Product Service 1-800-522-7658 33

the blower inlet (suction side) test port. Connect this tube to the 

“downstream” sampling port of the photometer. 

Acceptance 

The downstream average penetration shall not exceed 0.005 percent as 

measured by the photometer. 

Initial Certification 

The Precise HEPA-Filtered Glove Box has been leak tested at the factory 

with helium gas at positive 1" w.g. pressure; leaks cannot exceed 1 x 

10-3 cc/sec. The HEPA Filter Leak Test has been performed at the factory on 

both HEPA filters with no particulate leaks greater than .005 percent. The 

filtered glove box should be certified before use for the three tests listed 

under Re-Certification below. It is also recommended to perform the HEPA 

Filter Leak Test again should there be any damage caused during transport. 

Re-Certification 

Under normal operating conditions, the glove box should be re-certified at 

least annually if serviced. The certifier should perform the following tests. 

• Record airflow volume and glove box static pressure. Replace HEPA 

filters if pressure doubles or airflow is reduced to half the original 

volume. 

• Perform HEPA Filter Leak Test. 

• Perform a Smoke Test to determine proper dilution rate inside the glove 

box. Smoke removal should be completely purged in less than two 

minutes at full blower speed. 

Fluorescent Light Replacement 

1. Disconnect the power. Refer to Appendix A for replacement parts. 

2. Locate the light reflector located on the top of the glove box behind 

the front panel. 

3. Remove the light reflector support by removing two hex nuts located 

on both sides of the light reflector. 

4. Remove the old fluorescent lamp. 

5. Reinstall the new fluorescent lamp and light reflector by reversing the 

steps above. 
Chapter 6: Maintaining Your Glove Box

34 Product Service 1-800-522-7658 

Motorized Impeller Replacement 

The motorized impeller must be replaced as a complete unit. See Appendix A 

for replacement parts diagram. 

1. Unplug the motorized impeller from the electrical outlet. Refer to 

Appendix A for replacement parts. 

2. Remove the hose connecting the exhaust HEPA filter to the motorized 

impeller. 

3. Remove the motorized impeller and support braces from the light and the 

rear sheet metal façade. 

4. Reassemble the new motorized impeller by reversing the steps above. 

WARNING: High-speed blower. Never operate impeller 

with housing off. 

Speed Control Replacement 

1. Disconnect the power and remove the cover behind the front control 

panel where the speed control is located. 

2. Remove the two fasteners holding the speed control. Refer to Appendix 

A for replacement parts. 

3. Disconnect all wires leading to the speed control. Connect wires on new 

speed control in the same position as the old speed control. 

4. Reassemble to the system in the same position and with the same 

fasteners that were removed earlier. 

! 
Product Service 1-800-522-7658 35

Chapter 7:

Accessorizing Your Glove Box

There are many ways to accessorize and modify the glove box for your 

individual requirements. These include many different accessories listed in 

this chapter. 

1. Black Epoxy Flat Work Surface (Part #4882807) 

A solid epoxy resin 60" x 30" x 1.25" work surface is available to 

support the glove box. 

2. 30" Standard Base Cabinet (Part #9900200) 

Two optional base cabinets can be ordered to support the 60" x 30" 

work surface and glove box. Contact Labconco for additional sizes. 

3. Adjustable Height Base Stands with Attached Work Surface 

(Part #5235500 w/casters, Part #5235501 w/adjustable feet) 

These stands, 60" x 31", with attached black laminate work surfaces 

have an adjustable height range from 33" to 40" and include a lower 

shelf for supporting accessory equipment. 

4. Blower Foot Switch (Part #5241600) 

Provides a hands-free means to shut off power to the built-in blower. 

5. Bag Set for Bag-in/Bag-out HEPA Filter Replacement (Part #5241700) 

Set of two convenience bags and two safety straps for changing and 

disposing of one HEPA filter safely with a bag-in/bag-out procedure. 

6. Anti-Static Ionizer Fan (Part #5234400, 100/115V or 

Part #5234401, 230V) 

Used for weighing operations and attaches to the ceiling of the glove 

box. The ionizer fan circulates ionized air inside the glove box to 

reduce static charge. Ionizer Replacement Points (set of 8 emitter 

electrodes) are ordered separately (Part #5235100). See Appendix D 

for additional information. 

7. Balance Vibration Isolator (Part #5234600) 

Isolates building vibration and aids with balance weighing. Includes a 

20" x 13" x 2 1/4" marble slab with isolator pads and type 304 stainless 

steel top cover to protect the porous marble. 
Chapter 7: Accessorizing Your Glove Box

36 Product Service 1-800-522-7658 

8. Pressure Gauge for the Transfer Chamber (Part #5238100) 

Kit includes the pressure gauge, hose and fittings to add to the transfer 

chamber. Range 0 to 29 inches of mercury vacuum. 

9. Add-A-Valve Kit (Part #5225701) 

Includes the valve, O-ring, and nut to supply the main chamber or the 

transfer chamber to purge vacuum, fill gas, or connect a drying train. 

One each Identifier Label Set for valves (Part #5236600) is ordered 

separately. 

10. Vacuum Aspirator Kit 

Includes the valve, hose, canister, and vacuum pump for evacuating 

fluids around the work surface. 

Part # Descriptions 

5241800 Vacuum Aspirator Kit, 115V 

5241801 Vacuum Aspirator Kit, 230V 

11. Electrical Power Strips 

These convenience electrical power strips are available in US and 

international versions and plug into the interior duplex of the glove 

box. 

Part # Descriptions 

5211500 US Power Strip 115V, 4 Position, 5A 

5210600 UK Power Strip 250V, 6 Position, 2A 

5290400 European Power Strip 250V, 4 Position, 2A 

5332600 Australian Power Strip 250V, 6 Position, 2A 

5211501 Installation Tool 

12. Electrical 9 Pin Sealed Pass-Through Kit (Part #5075619) 

Includes sealed 9 pin (D-subminiature with male pins on one side and 

female pins on the other) connector and cord for data transfer to a 

typical printer outside the glove box from the balance. Note: Order 

the 9 pin installation kit separately which includes all the drill bits and 

hole saws for field installation (Part #5241100). 

13. Mechanical or Electrical Straight Pass-Through Kit 

(Part #5240700) 

Provides three ID pass-through ports with sizes of .230/.395, 

.230/.530, and .450/.705. Order the installation kit with drill bits 

separately (Part #5240800). Useful for sealing cords and plumbing. 

14. FilterMate Portable Exhausters and Filters for Secondary 

Filtration (see models and filters below) 

The FilterMate Portable Exhauster can successfully adsorb low level 

organic vapors, formaldehyde, ammonia, and secondary particulates 

via another HEPA filter. 
Chapter 7: Accessorizing Your Glove Box

Product Service 1-800-522-7658 37

Part # Descriptions 

3970000 115V FilterMate (HEPA only included) 

3970020 230V FilterMate (HEPA only included) 

3970001 115V FilterMate (Carbon Filter sold separately) 

3970021 230V FilterMate (Carbon Filter sold separately) 

3970002 115V FilterMate w/thimble (HEPA included) 

3970022 230V FilterMate w/thimble (HEPA included) 

3970003 115V FilterMate Combination HEPA/Carbon (order 

Carbon Filter separately) 

3970023 230V FilterMate Combination HEPA/Carbon (order 

Carbon Filter separately) 

3970004 115V FilterMate (Two Carbon Filters Sold separately) 

3970024 230V FilterMate (Two Carbon Filters sold separately) 

3707900 HEPA Filter for FilterMate 

3923400 Organic Carbon Filter for FilterMate, 12 lbs. 

3923401 Formaldehyde Carbon Filter for FilterMate, 14 lbs. 

3923402 Ammonia Carbon Filter for FilterMate, 16 lbs. 

3776002 Replacement Bag to Bag-In/Bag-Out HEPA Filter 

15. 5" Thimble Exhaust Connector for Connection to the FilterMate 

(Part #5241900) 

Connects to the top of the blower on the glove box for attaching the 

FilterMate Portable Exhauster. The thimble is necessary for 

balancing the blower in the glove box with the FilterMate Portable 

Exhauster. 

16. 6" Thimble Exhaust Connection to Building Exhaust (Part #5230200) 

Provides a 6" exhaust thimble connection to the blower on the glove 

box for exhausting to the outside. The thimble is necessary for 

balancing the blower on the roof with the glove box blower. 

17. 6" Hard Duct Connection to House Exhaust (Part #5230400) 

Provides a 6" hard duct connection for using a roof-mounted blower 

and by-passing the blower on the glove box. 

18. Remote Wall-Mount Blower Kit (Part #5229800) 

Provides a wall-mount bracket, 4" dia. x 6' long hose, hose clamps, 

and 8' power cord for moving the blower from the glove box and 

remotely mounting it to a wall within 5'. 

19. 6" Exhaust Dampers 

Exhaust dampers allow airflow adjustments to be made, maintaining 

proper airflow to roof-mounted remote blowers or building exhaust. 

Part # Descriptions 

3924000 6" Epoxy-Coated Steel In-Line Adjustable Damper 

4724200 6" PVC In-Line Adjustable Damper 
Chapter 7: Accessorizing Your Glove Box

38 Product Service 1-800-522-7658 

20. Remote Blowers 

Has a 1/4 hp direct drive motor and corrosion-resistant epoxy-coated 

steel housing and wheel with blower inlet of 6.00" ID. Outlet 

dimensions are 4.25" x 7.38" OD. 

CFM @ Static Pressure-Inches of H2O 

S.P. 0.0" 0.125" 0.25" 0.50" 0.75" 0.87" 

CFM 595 560 515 420 300 167 

Part # Description 

4863500 Remote Blower, 115 V, 60 Hz. 4.4 amps 

4863501 Remote Blower, 115/230 V, 50 Hz, 5.6/2.8 amps 

Figure 7-1 

All dimensions in inches 

21. Positive Pressure Conversion Kit (Part #5236900) 

Converts integral glove box blower from negative pressure operation 

to positive pressure. 

22. Recirculation Kit (Part #5236500) 

Allows the blower exhaust to be connected to the HEPA inlet filter 

for recirculation of the exhaust air. Useful with drying train 

components on the main chamber. 

23. Filtration Components for Moisture and Fume Removal 

The following components are useful in removing organics, acids, 

ammonia, moisture, and other gases as indicated. They are connected 

to the glove box using the #5225701 Add-A-Valve Kit. 

Part # Descriptions 

5248200 Vacuum Pump for Circulation, 115V, 60 Hz w/switch 

5248201 Vacuum Pump for Circulation, 230V, 50 Hz 

7815301 Canister with Stand for Traps 

7815200 Moisture & Solvent Trap Insert (molecular sieve) 

7814800 Acid Vapor Trap Insert 

7815000 Radioisotope Trap Insert 
Chapter 7: Accessorizing Your Glove Box

Product Service 1-800-522-7658 39

Part # Descriptions 

7995600 Ammonia Trap Insert 

5244100 Flowmeter, Visual 

5240100 Gas & Vacuum 0.2 Micron Filter 

24. Acrylic Viewing Window with Frame (Part #5236100) 

Acrylic window, 1/2" thick, for those users that prefer acrylic over the 

standard laminated safety glass (includes metal frame). 

25. Guardian Jr.TMAirflow Monitor (Part #5242000) 

Audio and visual airflow monitor is especially useful for installations 

that exhaust to the outside as it provides feedback on the roofmounted blower status. Monitor mounts to the top of the control 

panel. 

26. Interior Storage Shelf Kits 

Provide convenient storage in the rear or the left side of the glove box 

to maximize space. Additional hanging shelf kits (sold separately) 

can hang from the rear interior shelf kit. Each shelf kit adds 240 

square inches of usable space. Will support a large 15.2" x 4.8" x 

3.7" tissue box. 

Part # Descriptions 

5235000 Rear Storage Shelf Kit 

5235001 Side Storage Shelf Kit 

27. IV Bar Kit (Part #5242100) 

Provides a bar for supporting IV bags. Must be used with one of the 

interior storage shelf kits 5235000 or 5235001. 

28. External Glove Port Caps (Part #5242200) 

Cap and clamp attaches to the glove ports to eliminate glove inflation 

during dilution or venting operations. Sold as a set of two. 

29. Hanging Shelf Kits 

All shelves require the 5235000 rear interior storage shelf kit to 

function. 

Part # Descriptions 

3925000 Consists of three utility shelves (coated steel) 

3927700 Bottle Holder (coated steel) 

3927800 Tissue Holder (coated steel) 

5247300 Bin Organizer, 5.4" (plastic) 

5247301 Bin Organizer, 7.4" (plastic) 

30. DOP Test Kit for HEPA Filters (Part #5242400) 

Includes the connections and hoses to carry out the HEPA Filter Leak 

Test documented in Chapter 6 of this manual. 
Chapter 7: Accessorizing Your Glove Box

40 Product Service 1-800-522-7658 

31. Drying Train Tubing Kit 

Includes the (FEP) tubing and plumbing fittings for connecting typical 

moisture, and other gas fume removal components (see item 23 

above). Fluorinated ethylene-propylene (FEP) tubing has very low 

moisture absorption. 

Part # Descriptions 

5242500 Drying Train Tubing Kit; Moisture, Solvents, 

and Radioisotopes 

32. HEPA and ULPA Filters 

HEPA filters come standard with the glove box. ULPA filters may be 

substituted as an upgrade. 

Part # Descriptions 

5225105 Replacement Standard HEPA Filter, 99.99% 

efficient on 0.3 micron particles 

5225106 Optional ULPA Filter, 99.999% efficient on 0.3 

micron particles 

33. Gloves (see Appendix A: Replacement Parts for glove sizes and 

material types) 

Neoprene gloves are the most resistant to abrasion and tearing. Butyl 

gloves provide higher impermeability and improved dexterity. 

Hypalon gloves are made with a mixture of neoprene and butyl rubber 

to provide an intermediate level of neoprene and butyl features. 

34. Transfer Chamber High Vacuum Sleeve (Part #5236200) 

An internal steel reinforced transfer chamber sleeve is required if a 

vacuum will be pulled in the transfer chamber. 

35. Waste Chute (Part #5245800) 

The glove box can be configured to add a waste chute from which a 

waste bag can hang. Waste bags and bands can be ordered from your 

laboratory dealer. The minimum waste bag size is 8.5" wide. 

36. Exterior Transfer Chamber Shelf (Part #5245900) 

A powder-coated steel shelf, 10.7" wide x 15.1" deep, can be attached 

to the top of the transfer chamber. The exterior transfer chamber shelf 

maximizes work area, useful for printers, laboratory supplies, and 

notebooks. 
Product Service 1-800-522-7658 41

Chapter 8:

Troubleshooting

Refer to the following table if the Precise HEPA-Filtered Glove Box fails to 

operate properly. If the suggested corrective actions do not solve your 

problem, contact Labconco for additional assistance. 

PROBLEM CAUSE CORRECTIVE ACTION 

Blower won’t operate. Unit not plugged into 

outlet. 

Plug the glove box into appropriate electrical 

service. 

 Circuit breaker(s) or 

Ground Fault Interrupter. 

Reset circuit breaker. 

 Blower wiring is 

disconnected. 

Inspect blower wiring per wiring diagram on 

unit. 

 Blower switch is 

defective. 

Replace switch if no voltage at blower. 

 Motorized impeller or 

blower is defective. 

Replace motorized impeller or blower. See 

Chapter 6. 

Unit not plugged into 

outlet. 

Blower and lights won’t Plug unit into appropriate electrical service. 

operate. 

Circuit breaker(s) tripped. Reset or replace circuit breaker. 

Lights do not work. Lamp not installed 

properly. 

Inspect lamp installation. 

 Lamp wiring 

disconnected. 

Inspect lamp wiring. 

 Defective lamp. Replace lamp. 

 Light switch is defective. Replace light switch. 

 Defective electronic 

ballast. 

Replace ballast. 
42 Product Service 1-800-522-7658 

Appendix A:

Replacement Parts

The components that are available for the Precise HEPA-Filtered Glove Box 

are listed. The parts shown are the most commonly requested. If other parts 

are required, please contact Product Service. 

Item Qty. Part Number Description 

1A 1ea 5232200 Framed Glass Window (standard) 

1B 1ea 5236100 Framed Acrylic Window (optional) 

2A 1ea 1230700 Receptacle, 115V Duplex 

2B 1ea 1283900 Receptacle, 230V Duplex 

2C 1ea 5232900 Sealed Duplex, 115V Complete 

2D 1ea 5232901 Sealed Duplex, 230V Complete 

2E 1ea 5233400 Wallplate Duplex Gray 

3A 1pr 1640600 9 3⁄4 size Neoprene Gloves (standard) 

3B 1pr 1640601 9 3⁄4 size Butyl Gloves 

3C 1pr 1640602 9 3⁄4 size Hypalon Gloves 

3D 1pr 1640500 8 1⁄2 size Neoprene Gloves 

3E 1pr 1640501 8 1⁄2 size Butyl Gloves 

3F 1pr 1640502 8 1⁄2 size Hypalon Gloves 

3G 2ea 1640000 O-Rings 

3H 2ea 1965600 Clamp – O-Ring Gloves 

4 1ea 1952500 Pressure Differential Gauge 

5 1ea 1307000 Switch, Rocker (2 Position) 

6A 1ea 3704402 Blower Speed Control, 115V 

6B 1ea 1931500 Knob, Speed Control 

6C 1ea 5229400 Extension, Speed Control 

6D 2ea 1890302 Set Screw, Extension #8-32 x .12" 

6E 1ea 3704403 Blower Speed Control 230V 

7A 2ea 5225105 HEPA Filter 99.99% 

7B 2ea 5225106 ULPA Filter 99.999% 

7C 1ea 5241700 Bag-In/Bag-Out Kit 

7D 12ea 1905617 Nut, Keps #10-24 

8A 1ea 5227300 Motorized Impeller, 115V (also used on 100V units) 

8B 1ea 5227301 Motorized Impeller, 230V 

9A 1ea 5222700 Fluorescent Bulb, 8" Circleline FC8T9/CW, 22 Watt, 4 Pin 

9B 1ea 5222800 Ballast, 115V 60 Hz 

9C 1ea 5222801 Ballast, 230V 50 Hz (also used on 100V, 50 Hz) 

9D 1ea 5222802 Ballast, 230V 60 Hz (also used on 100V, 60 Hz) 

9E 1ea 5242900 Transformer, 230V to 100V (100V models only) 

10A 1ea 1305800 Inlet Power Cord, 115V 

10B 1ea 1305900 Inlet Power Cord, 230V 

11A 1ea 5226400 Inner Transfer Door w/attached gasket 

11B 1ea 5226500 Outer Transfer Door w/attached gasket 

12A 1ea 1327204 Circuit Breaker, 5A, 115V 

12B 4ea 1327205 Circuit Breaker, 2A, 230V 

12C 1ea 1327200 Circuit Breaker, 3A, 115V 

13A REF. 5226900 Wiring Diagram, 115V 

13B REF. 5226901 Wiring Diagram, 230V 
Appendix A: Replacement Parts

Product Service 1-800-522-7658 43

Figure A-1 

Replacement Parts for Precise HEPA-Filtered Glove Box 
44 Product Service 1-800-522-7658 

Appendix B:

Dimensions

See the following dimensions and exhaust options for the Precise HEPAFiltered Glove Boxes. All dimensions are in inches. 

Figure B-1 
Product Service 1-800-522-7658 45

Appendix C:

Specifications

This Appendix contains technical information about all the glove boxes 

including electrical specifications and environmental operating conditions. 

• 8 Amps, 115V, 50/60 Hz or 4 Amps, 230V, 50/60 Hz or 8 Amps, 100V, 

50/60 Hz, Precise HEPA-Filtered Glove Box. 

Environmental Conditions 

• Indoor use only. 

• Maximum altitude: 6562 feet (2000 meters). 

• Ambient temperature range: 41° to 104°F (5° to 40°C). 

• Maximum relative humidity: 80% for temperatures up to 88°F (31°C), 

decreasing linearly to 50% relative humidity at 104°F (40°C). 

• Main supply voltage fluctuations not to exceed ±10% of the nominal 

voltage. 

• Transient over-voltages according to Installation Categories II (Overvoltage Categories per IEC 1010). Temporary voltage spikes on the 

AC input line that may be as high as 1500V for 115V models and 

2500V for 230V models are allowed. 

• Used in an environment of Pollution degrees 2 (i.e., where normally 

only non-conductive atmospheres are present). Occasionally, 

however, a temporary conductivity caused by condensation must be 

expected, in accordance with IEC 664. 
46 Product Service 1-800-522-7658 

Appendix D:

Ionizer Fan Performance Criteria

Static Electricity Test 

The tests were performed by monitoring the level of static electricity found on the work 

surface and interior surfaces within the glove box. Static electricity levels were measured 

with an AlphaLab Surface DC Voltmeter http://scientificmeter.com/surface_dc.htm. 

A DESCO Emit Mini Zero Volt Ionizer http://www.descoemit.com/ViewProduct.aspx?pid=

50661&h=1323 was installed in the glove box. The DESCO ionizer is a dual steady state DC 

auto balancing bench top ionizer. The ionizer helps to neutralize static electricity on 

surrounding surfaces. The time required by the ionizer to neutralize static electricity will 

be the shortest when objects are within 12" to 36" directly in front of the ionizer. The time 

for neutralization increases as the distance from the ionizer increases. The time required 

for sufficient electrostatic decay will also vary depending upon user application, moisture 

levels within the supply air and the level of cleanliness within the glove box interior. 
Appendix D: Ionizer Fan Performance Criteria

Product Service 1-800-522-7658 47

Test Point Locations and Voltage Levels 

Metal 

Housing 

Inlet 

HEPA 

Metal 

Housing 

Exhaust 

HEPA 

Metal 

Transfer 

Chamber 

Handle 

Back 

Interior 

Surface 

Left 

Interior 

Surface 

Right 

Interior 

Surface 

Interior 

Lamp 

Window 

Center 

Work 

Surface 

Electrostatic with 

Glove Box Blower & 

Ionizer Fan OFF 

+ 420 + 220 + 734 + 375 + 106 + 151 + 242 + 153 

100 CFM Blower & 

Ionizer Fan ON 

After 1 Hour 

+ 96 + 114 + 290 + 93 + 45 + 52 + 46 + 35 

100 CFM Blower & 

Ionizer Fan ON 

After 2 Hours 

+ 28 + 58 + 51 - 52 - 15 - 20 - 28 - 22 

The mini zero volt ionizer’s neutralization time will be shortest when objects are 

approximately 12" to 36" directly in front of the ionizer and will increase as the 

distance from the ionizer increases. Set the fan speed switch on the side of the 

ionizer to the LOW or HIGH position. Higher airflow will result in faster 

neutralization rates. Position the ionizer so that the maximum airflow is directed 

at the items or area to be neutralized. The LED will turn on during power up and 

remain GREEN during normal operation. The ionizer has a grounding jack and 

must have a good earth ground to maintain proper balance. Always plug the AC 

power adaptor into the ionizer and then into the appropriate AC power source. 

The ionizer has no On/Off switch so it should be running as soon as it is plugged 

in. The ionizer is designed to run off of 24VDC ±10% 300mA. 

The balance adjustment for the ionizer can be accomplished by inserting a small 

screwdriver or trimmer adjustment tool into the balance adjustment hole located 

on the side of the unit. To increase the output in a positive direction, turn the 

potentiometer in a clockwise direction. Conversely, to increase the output in a 

negative direction, turn the potentiometer in a counter clockwise direction. It is 

recommended that you use a surface DC voltmeter when adjusting the balance of 

the ionizer. 

Under normal conditions, the ionizer will attract dirt and dust (especially on the 

emitter electrodes). To maintain optimum neutralization efficiency and operation, 

cleaning with isopropyl alcohol should be performed on a regular basis. If the 

performance of the ionizer degrades because of dirty or corroded points, the LED 

on the front of the unit will turn YELLOW; the audible alarm will sound 

continuously. Under this condition, the unit is not able to maintain balanced 

ionization. The input power cord must be disconnected before the unit is opened 

for maintenance. The emitter electrodes should be cleaned using the alcohol 

cleaners included or a swab wet with isopropyl alcohol. Unscrew the 4 screws on 

the back of the unit and then remove the screen. After cleaning the emitter 

electrodes, reinstall the screen and 4 screws. The emitter electrodes should not 

require replacement during the life of the unit with normal handling. Verify the 

balance of the ionizer with a surface DC voltmeter after cleaning. 

Static Electricity Test Results (VOLTS) on the Glove Box 

(Neutralization is best between +/-100V) 
48 Product Service 1-800-522-7658 

Appendix E:

Conversions

Pressure Conversions 

To 

From 

mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar 

mmHg 1 .03937 .5353 .04461 .00132 .01934 .00136 .1333 .0013 

in.Hg 25.40 1 13.60 1.133 .03342 .4912 .03453 3.387 .0339 

in.H2O 1.868 .07355 1 .08333 .00246 .03612 .00254 .2490 .0025 

ft.H2O 22.42 .8826 12 1 .02950 .4334 .03048 2.988 .0299 

atm 760 29.92 406.8 33.90 1 14.70 1.033 101.3 1.013 

lb/in.2 51.71 2.036 27.69 2.307 .06805 1 .07031 6.895 .0689 

Kg/cm2 735.6 28.96 393.7 32.81 .9678 14.22 1 98.05 .981 

kPa 7.500 .2953 4.016 .3347 .00987 .1451 .0102 1 .01 

bar 750 29.53 401.6 33.47 .987 14.51 1.02 100 1 

Flow Rate Conversions 

To 

From 

lit/sec gal/min ft3/sec ft3/min bbl/hr bbl/day 

lit/sec 1 15.85 0.03532 2.119 22.66 543.8 

gal/min 0.06309 1 0.00223 0.1337 1.429 34.30 

ft3/sec 28.32 448.8 1 60 641.1 1.54x104

ft3/min 0.4719 7.481 0.01667 1 10.69 256.5 

bbl/hr 0.04415 0.6997 0.00156 0.09359 1 24 

bbl/day 0.00184 0.02917 6.50x105

 0.00390 0.04167 1 
Product Service 1-800-522-7658 49

Appendix F:

References

Many excellent reference texts and booklets are currently available. The 

following is a brief listing: 

Pharmaceutical Isolators, A Guide to their application design and control. 

Pharmaceutical Press 2004, Editors: Midcalf, Phillips, Neiger, and Coles. 

Isolation Technology, A Practical Guide, 2004 CRC Press, 2nd Edition, 

www.crcpress.com 

International Standard ISO 10648-2 Containment Enclosures 

• Classification according to leak tightness and associated checking 

methods. 

International Standard ISO 14644-7 Clean Rooms and associated controlled 

environments 

• Separate devise such as glove boxes and isolators. 

NIOSH Pocket Guide to Chemical Hazards 

U.S. Dept of Health and Human Resources 

Centers for Disease Control and Prevention 

National Institute for Occupational Safety and Health 
50 Product Service 1-800-522-7658 

DECLARATION OF CONFORMITY 

Application Council Directive(s): 73/23/EEC, 89/336/EEC, 2002/95/EC (ROHS), 

 2002/96/EC (WEEE) 

Standard(s) to which conformity is declared: EN61010-1, EN61326-1 

Manufacturer’s Name: Labconco Corporation 

Manufacturer’s Address: 8811 Prospect Avenue 

 Kansas City, MO 64132 USA 

Importer’s Name: See Shipping/Customs Documents 

Importer’s Address: See Shipping/Customs Documents for your equipment 

Type of Equipment: Laboratory Equipment Precise Glove Boxes 

5220000 Series Precise Basic Glove Box 

5220100 Series Precise Controlled Atmosphere Glove Box 

5220200 Series Precise HEPA-Filtered Glove Box 

5220300 Series XPert Weigh Box 

Serial No.: Various – See Individual Declaration 

Year of Manufacture: 2006 and subsequent 

I, the undersigned, hereby declare that the equipment specified above conforms to the 

above Directive(s) and Standard(s). 

 

 See individual Declaration of Conformity which 

 will be signed by the importer for your country. 

Place: _______________________________________ 

 (Signature) 

Date: _______________________________________ 

 (Full Name) 

 _______________________________________ 

 (Position) 

Labconco P/N 36960-60, REV B, ECO E026