Such a program of planned preventive
maintenance, involving a thorough cleaning, check
ing and adjustment of mechanisms is recommended.

This work should be performed by qualified per-
sonnel with the proper training and equipment.
Cambridge Instruments, or your Cambridge In-
struments service dealer can arrange this service.
Should unexpected trouble be experienced with
your instrument — proceed as follows:
© Contact your Cambridge Instruments dealer, He

may be able to suggest simple remedies to correct
the apparent difficulty without having to send the
instrument out for servicing.

© It however, it becomes necessary to return your
instrument, pack the microscope carefully in a crush
resistant carton with at least three inches of shock
absorbing material surrounding it to prevent in-
transit damage. Should a suitable carton not be
available, one may be ordered from the factory

@ A detailed letter should be included* in the shipping
carton, preferably fastened to the instrument,
describing the trouble experienced. This information
will enable the service technician to make required
repairs promptly

“Please mark on the shipping container FIRST

CLASS LETTER ENCLOSED. First Class postage must

be paid only on the letter. The carton will be accepted

at standard package rates.


accomplished by removing one of the eyepieces
and looking down the eyepiece tube at the
illuminated back aperture of the objective. While
observing this aperture, close down the condenser
aperture diaphragm until the size of the objective
back aperture is reduced to about 75 percent of its
onginal size

@ Specimens with contrast so low that the specimen
is invisible or barely visible often can be made
visible by closing down the condenser aperture
diaphragm to a pinhole size.

@ An alternate and otten superior method of
improving contrast is to use oblique illumination

This is accomplished by covering ¥2 or more of the
condenser aperture with an opaque piece of paper.

© Both of these methods of contrast enhancement
introduce diffraction effects which reduce the ability
of the microscope to resolve the fine details of the
specimen and are not recommended where a critical
examination of the specimen is required. Instead,
the technique ot phase contrast microscopy should
be employed.

Accessories tor converting your standard

Galen III Microscope into a phase contrast microscope
are available.

5.0 Maintenance

Your Galen III Microscope has been engineered for

long life with a minimum of maintenance required. In

general, routine maintenance on your part is limited to

keeping the microscope clean. Two important steps to

remember are

© Never leave the microscope with any of the
objectives or eyepieces removed.

© Always protect the microscope with the dust cover
when not in use

5.1

Cleaning

Accumulated dirt on the metal surtace should be

cleaned with a damp cloth. If this is inadequate, a mild

soap solution should be used.

The outer surfaces of the optics should be
inspected from time to time for dirt and dust. Dust is
best removed by an air stream from a rubber air bulb
Remove more persistent dirt with a soft cloth or cotton
swab dampened in a mild solution of soap and water,
alcohol, or as a last resort, xylol. A small-amount of
absorbent cotton wound on the end of a tapered stick
makes a handy tool for cleaning recessed optical
surfaces. Avoid excessive use of solvents as this may
cause problems with cemented optics, or the flowing
solvent may pick up grease from the mounts, making
cleaning more difficult

Clean immersion objectives immediately after use
by removing the oil with lens tissue or a clean soft
cloth

Occasionally the underside of the mechanical
stage fingers may become coated with immersion oil
and require cleaning.

Detach the mechanical stage by removing the
three screws on the underside of the stage table. When
cleaning the oil from the underside of the stage fingers,
be careful not to remove or contaminate the lubncant
in the stage slide ways. Reinstall the mechanical stage
on the stage table with great care to prevent damage to
the slide way, rack, or pinion
5.2
Lamp Replacement
The only component in your Galen III Microscope
which requires periodic replacement is the illuminator
lamp. Before replacement, UNPLUG the instrument
Move the mechanical stage to the extreme right
position and carefully place the microscope on its lett
side. Open the lamp window in the bottom plate by
pulling the window knob. After the lamp has cooled,
carefully remove it from its socket and replace with a
new lamp. When replacing the Tungsten-Halogen
lamp, be careful not to touch the new lamp with your
fingers.

CAUTION
Never operate the microscope illuminator unless
the lamp window is securely in place.
5.3
Service
All optical and mechanical equipment requires
periodic servicing to keep it performing properly and
compensate for normal wear. Anticipating this need by
establishing a schedule of regular preventive
maintenance will help to assure long life and sustained
optimum performance by your instrument. It will also
help prevent unexpected trouble and the necessity of
having the instrument serviced at inconvenient times.


1.0 Introduction

Your Cambridge Instruments Galen TM II] Protession-
al Microscope is the most recent addition to our
line of professional laboratory microscopes for the
biological and medical sciences. Its exclusive
design features, professional quality, and full range
of accessories make it a truly versatile instrument
for most laboratory applications.

Optional accessories allow you to adapt your
Galen III Professional Microscope to Bright Field,
Dark Field, Phase Contrast, Polarized Light, or
Photomicrography.

The Galen III Professional Microscope is a
member of the Cambridge Instruments family of
guality microscopes, which includes the
sophisticated StereoZoom® , and MicroZoom®
Microscopes for college and laboratory use.

Cambridge Instruments is a world leading
manufacturer of scientific and optical instrumenta-
tion, as well as quality ophthalmic instruments. The
Galen III Professional Microscope and all our in-
struments are backed by our worldwide sales and
service organizations.

I SS Ty
2.0 Unpacking

Remove the microscope components from the packing
carton and, before any of the packing material is
discarded, check the components against the following
standard equipment list

1. Stand, which includes supporting arm, focusing
mechanism, mechanical stage, nosepiece and mirror
or integral illumination system

2. Viewing head (monocular, binocular or triocular
depending upon the model ordered).

3. Objectives as ordered.
4. Eyepiece(s) as ordered.

S. Abbe condenser with aperture diaphragm and
swing-out filter holder (bright field models).

Blue filter

Plastic cover.

fo

on

Immersion oil

9. Spare Lamp.

Optional accessory items such as phase contrast
dark field condenser, carrying case and camera
accessones are not shipped as part of the standard
equipment. These items are shipped in separate
packages.


3.0 Preparation

3.1

Objectives

Remove the objective lens units from their plastic
containers and attach to the revolving nosepiece holes
from the lowest magnification to the highest, in a
clockwise fashion. Then move the 10X objective into
working position. Always rotate the nosepiece by using
the knurled nosepiece ring

3.2

Viewing Head

Loosen the viewing head clamp screw a turn or two
and carefully seat the head onto the microscope arm.
Lock the head in any desired orientation by tightening
the clamp screw.

3.3

Condenser

Loosen the condenser clamp screw a turn or two and
insert the condenser from below. Position the
condenser with the handle of the aperture diaphragm
conveniently accessible. Tighten the condenser clamp
screw finger-tight. To eliminate light irregularity when
using low-power objectives such as 4X, and 10X, raise
and lower the condenser using the condenser
adjustment knob.

3.4

Filter

The filter holder is attached to the condenser by a pivot
allowing you to swing the filter into or out of the light
path, as desired. Swing the filter holder outward and
insert the filter.

3.5

Eyepieces

Place eyepiece(s) into eyepiece tub The built-in
eyeguards may be pulled out to reduce stray light. This
completes assembly of the microscope.

REL Raia Seibel
4.0 Operation

Plug the microscope power cord into a suitable
grounded electrical outlet. A grounded 3-wire cord is
provided.

CAUTION

As a safety precaution, the power cord has been
grounded to the microscope base. Never use a 2-
prong adapter between the power cord and the
power source; it will render this feature ineffective.

Move the illuminator switch to the “on” position. To
obtain the desired illumination slide the light control
rheostat. The degree of light intensity depends on
individual conditions such as specimen contrast,
objective magnification, eye accommodation, etc. Too
little or too much light is not recommended. The
“energy saving” illuminator indicator lamp remains lit
when the illuminator is in use.
44
Auto Focus Stop
Before focusing, turn the auto focus stop knob
counterclockwise for free stage motion. After focusing,
if the auto focus stop is required (to prevent the stage
from moving past a certain point), tighten the auto
focus knob by turning it clockwise.

4.2
Binocular & Triocular Head

Setting Interpupillary Distance Using two hands,
grasp the head face plate moving the eyepiece tubes
together or apart until the full field of view is
simultaneously visible through both eyepieces. An
improper adjustment will cause operator fatigue and
will upset the objective partocality. Once the proper
setting is achieved, note the reading on the interocular
scale. Rotate the diopter ring on the right eyepiece tube
to the same reading as noted on the interocular scale.
Close the left eye, or cover the left eyepiece with
opaque maierial, and carefully focus the microscope
with the fine adjustment knob. Close the right eye, and
without touching either adjustment knob, rotate the lett
diopter ring until the specimen is in sharp focus for the
lett eye.
If the interocular and diopter ring adjustments
have been made properly, you will
© Note the same state of focus in the right and left
eyepieces whenever any specimen is brought into
focus with either the coarse or fine adjustment knob
© Comfortably see the full field of view through both
eyepieces while holding the head in a fixed position.
© Need only a fine adjustment “touch-up” to bring the
specimen into sharp focus as one objective on the
nosepiece is exchanged for another.


If these effects are not observed, go back and
repeat the adjustments. To eliminate the need for
repeating these adjustments each time you use the
microscope, memorize the scale values. Wearers of
eyeglasses with strong corrections may require slightly
different optimum settings for the diopter rings trom
those obtained by the above procedure. The previous
instructions assume that both eyes have about the
same degree of correction. However, if you have one
eye requiring much greater correction than the other.
use the following procedure: Set the diopter ring on the
eyepiece tube which corresponds to your “strong” eye
(the one which is best or closest to normal) to the
reading noted on the interocular scale. Close the
“weak” eye and caretully focus the microscope with
the fine adjustment knob. Now close the ‘strong’ eye
and, without touching either adjustment knob, rotate
the diopter corresponding to your “weak” eye until the
specimen is in sharp focus.

4.3

Focusing

Coarse Adjustment - Turn the coarse adjustment
knob counterclockwise. The stage will stop at the
bottom of its travel. Turning the knob in the opposite
direction will raise the stage.

Place a specimen slide on the center of the stage
Using a 10X objective and a 10X eyepiece for
preliminary observation, bring the specimen into focus
with the coarse adjustment knob

CAUTION

To prevent damage to the specimen and objective,

use the following focusing procedure:

© Look at the slide directly (not through the
eyepieces) and raise the stage by slowly turning
the coarse adjustment knob clockwise. Continue
raising the stage until the cover glass nearly
touches the objective or the stage reaches its
upper limit.

® Look at the specimen through the eyepieces and
turn the coarse adjustment knob counter-
clockwise to lower the stage, bringing the
specimen into focus.

Then adjust the autofocus stop knob as described
in Section 4.1. The other objectives will remain in
parfocality with no danger of touching the specimen
slide. If the stage moves too freely, using the coarse
adjustment tension knob, adjust the tension, turning
clockwise to tighten, counter-clockwise to loosen

4.4
Blue Daylight Filter

If the light is excessively yellow, place the blue
daylight filter in the condenser filter holder

4.5
Mechanical Stage

The specimen movement range is about 50mm x
75mm. This is accomplished by rotating the

nobs.

mechanical stage contr
Reading vernier.

mis the main scale, “n” is the vernier scale

1 division of n =

Note Fig. 1. The n scale is positioned between
12mm and ‘Tamm of m. Find the nearest point where m
and n are aligned to determine the added millimeter
division.

Example

The figure shows 20 of m is aligned with 8 of n,
which is 0.8mm. Therefore, the reading is 12mm of m
plus 0.8mm of n, or 12.8mm. This reading is the sum of
the m indication, plus the n reading at the closest

aligned point.

CAUTION
Retract the mechanical stage fully when you've
finished using the microscope. If the mechanical
stage is fully extended either way, it is possible to
damage the stage when attempting to put the
microscope into the carrying case.

MAIN SCALE “m”

VERNIER SCALE “n”

Fig. 1

4.6

For Best Results

If you have carefully followed the preceding

instructions, the microscope can be operated over a

wide range of conditions with a minimum of

adjustments. There are variables, however, which you
might want to change from specimen to specimen.

® The light level should be adjusted to the lowest
level compatible with comfortable viewing; this will
greatly extend the life of the lamp. The optimum lamp
setting will change depending upon specimen
density and objective magnification.

@ When specimens of inherently low contrast are
examined, contrast usually can be improved by
reducing the condenser numerical aperture (NA) to
about 75 percent of the objective NA. This is best