pH/mV/ISE/Temperature

Bench Meters

HI5221 & HI5222

INSTRUCTION MANUAL
Thank you for choosing a Hanna Instruments product.

Please read this instruction manual carefully before using this instrument. 

This manual will provide you with the necessary information for correct 

use of this instrument, as well as a precise idea of its versatility.

If you need additional technical information, do not hesitate to 

e-mail us at tech@hannainst.com or view our worldwide contact 

list for a Hanna representative near you at www.hannainst.com.

Dear

Customer

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent 

of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
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TABLE OF CONTENTS

PRELIMINARY EXAMINATION ...............................................................................................

GENERAL DESCRIPTION .......................................................................................................

FUNCTIONAL DESCRIPTION ..................................................................................................

SPECIFICATIONS .................................................................................................................

OPERATIONAL GUIDE ..........................................................................................................

DISPLAYING MODES...........................................................................................................

SYSTEM SETUP ...................................................................................................................

pH SETUP ..........................................................................................................................

mV SETUP .........................................................................................................................

ISE SETUP (HI5222 only) ..................................................................................................

pH CALIBRATION ................................................................................................................

pH MEASUREMENT .............................................................................................................

mV & Relative mV MEASUREMENTS .....................................................................................

ISE CALIBRATION (HI5222 only) .........................................................................................

ISE MEASUREMENT (HI5222 only) .....................................................................................

TEMPERATURE CALIBRATION ...............................................................................................

LOGGING ............................................................................................................................

PC INTERFACE ....................................................................................................................

ADDITIONAL INFORMATION .................................................................................................

pH BUFFER TEMPERATURE DEPENDENCE .............................................................................

ELECTRODE CONDITIONING AND MAINTENANCE ....................................................................

TROUBLESHOOTING GUIDE .................................................................................................

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ........................................................

ACCESSORIES .....................................................................................................................
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The meters are supplied complete with:

• HI1131B Glass body Combination pH Electrode

• HI7662-W Temperature probe

• HI7082S Electrolyte Solution

• HI76404W Electrode Holder

• pH Calibration Solutions Kit

• Capillary dropper pipette

• 12 Vdc Power Adapter

• Instruction Manual and Quick Reference Guide

 •Certificate

HI5221-01 and HI5222-01 are supplied with 12 Vdc/120 Vac adapter.

HI5221-02 and HI5222-02 are supplied with 12 Vdc/230 Vac adapter.

Note: Save all packing material until you are sure that the instrument works properly.

Any defective item must be returned in the original packing with the supplied accessories.

Remove the instrument from the packing material and examine it carefully to make sure 

that no damage has occurred during shipping. If there is any damage, notify your Dealer or 

the nearest Hanna Customer Service Center.

PRELIMINARY EXAMINATION
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HI5221 and HI5222 are professional bench meters with a color graphic LCD for pH (with Calibration 

Check), ORP (Oxidation Reduction Potential), ISE (HI5222 only) and Temperature measurements.

The display can be configured as a single channel or dual channel display (HI5222 only). Each channel 

can be configured as pH, mV, Relative mV or ISE (HI5222 only).

The main features of the instruments are:

• Single (HI5221) or two (HI5222) input channels;

• Capacitive touch keypad;

• pH calibration using up to five pH buffers: choose from the list of Hanna buffers (pH 1.68, 3.00, 

4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) or custom buffers (up to 5 custom buffers);

• ISE calibration using up to five standards: choose from the list of nominal standard values

(e.g. for ppm: 0.010, 0.100, 1.00, 10.0, 100, 1000, 10000 ppm) and/or from 5 user-supplied 

custom standard values (up to 5 custom solutions);

• AutoHold feature to freeze first stable reading on the LCD;

• Two selectable alarm limits;

• User Selectable logging modes: Automatic logging, Manual logging with or without AutoHold;

• Continuous Lot logging directly on meter, with selectable log interval: Store up to 100,000 total 

data points; 

• Up to 100 logging lots; a single lot can have up to 50,000 data points; HI5222 (same for each 

measurement channel). Log files include GLP information;

• Basic Measurement can be viewed with detailed GLP information, or with a Graph or a Log History;

• User-friendly interface on large color graphic LCD (240 x 320 pixels) with user selectable color 

palette;

• PC interface via USB; download logged data to PC or use for Real time logging (HI92000

PC application required);

• Dedicated Help key with contextual message;

• Profile feature: store up to ten different user setup (HI5222- five on each channel).

GENERAL DESCRIPTION
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HI5221 DESCRIPTION

FRONT PANEL

REAR PANEL

1) Liquid Crystal Display (LCD)

2) Capacitive touch keypad

3) ON/OFF switch

4) Power adapter socket

5) USB connector

6) BNC electrode connector for pH/ORP measurements

7) Temperature probe socket

8) Reference input socket

FUNCTIONAL DESCRIPTION
7

HI5222 DESCRIPTION

FRONT PANEL

1) Liquid Crystal Display (LCD)

2) Capacitive touch keypad

3) ON/OFF switch

4) Power adapter socket

5) USB connector

6) BNC electrode connector for pH/ORP/ISE measurements (Channel 1)

7) Temperature probe socket (Channel 1)

8) Reference input socket (Channel 1)

9) Reference input socket (Channel 2)

10) Temperature probe socket (Channel 2)

11) BNC electrode connector for pH/ORP/ISE measurements (Channel 2)

1

2

pH ISE

3

11 10 9 8 7 6

4 5

Ref.

Temp. pH/OR P/ISE

Channel 1

USB

ON

OFF

pH/OR P/ISE Temp.

Channel 2 Ref.

HI 5222

Power

12Vdc

+ -

REAR PANEL

FUNCTIONAL DESCRIPTION
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KEYBOARD DESCRIPTION

FUNCTION KEYS

VIRTUAL KEYS

The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow 

you to perform the displayed function, depending on the current menu (e.g. for HI5222 ,

 and in Measure mode).

Note: All the virtual keys are assigned to the highlighted channel (HI5222 only).

LCD GENERAL DESCRIPTION

To enter/exit calibration mode.

To select the desired measurement mode, pH, mV, Rel mV (or ISE – HI5222 only).

To enter Setup (System, pH, mV or ISE) and to access Log Recall function.

To obtain general information about the selected option/operation.

FUNCTIONAL DESCRIPTION
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HI5221 HI5222

Range

-2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000 pH

±2000.0 mV

- e.g. 10-7 to 10M, 0.005 to 105

 ppm,

5·10–7 to 5·107 conc.

-20.0 to 120.0 °C / -4.0 to 248.0 °F / 253.2 to 393.2 K

Resolution

0.1 pH / 0.01 pH / 0.001 pH

0.1 mV

- 1, 2, 3 significants digit

0.1 °C / 0.1 °F / 0.1 K

Accuracy

@25 °C / 77 °F

±0.1 pH / ±0.01 pH / ±0.002 pH ±1 last significant digit

±0.2 mV ±1 last significant digit

- ±0.5% (monovalent ions)

±1% (divalent ions)

±0.2 °C / ±0.4 °F / ±0.2 K

Relative mV offset range ±2000.0 mV

pH Calibration Up to five points calibration, 8 standard buffers available

(pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and 5 custom buffers

ISE Calibration -

Up to five points calibration,

seven fixed standard solutions available,

for each measurement unit,

and five custom solutions

Temperature User Calibration 3 points (0, 50, 100 °C)

Input Channel 1 pH/mV 2 pH/mV/ISE

Temperature Compensation Manual or Automatic

GLP Electrode offset / slope, calibration points, calibration time stamp, date, time and 

buffers/standards used

Logging Record Up to 100 lots, 50,000 records max/lot / maximum 100,000 data points/channel

Logging Interval 14 selectable between 1 second and 180 minutes

Logging Type Automatic, Manual and Auto Hold

pH Electrode HI1131B

Temperature Probe HI7662-W

PC Interface Opto-isolated USB

Input Impedance 1012 Ω

Display Color Graphic LCD (240 x 320 pixels)

Power Supply 12 Vdc adapter

Enviroment 0 - 50 °C / 32 - 122 °F / 273 - 323 K max. 95% RH non-condensing

Dimensions 160 x 231 x 94 mm (6.3 x 9.1 x 3.7”)

Weight 1.2 Kg (2.6 lbs.)

SPECIFICATIONS
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POWER CONNECTION

Plug the 12 Vdc adapter into the power supply socket.

Notes: These instruments use non-volatile memory to retain the pH, ISE calibrations and all 

other settings, even when unplugged.

Make sure a fuse protects the main line.

ELECTRODE AND PROBE CONNECTIONS

For pH or ORP measurements, connect a pH/ORP electrode with internal reference to the BNC 

connector located on the rear panel of the instrument (to the desired channel - HI5222 only).

For ISE measurements, (HI5222 only) connect, to the desired channel, an ISE electrode with internal 

reference to the BNC connector located on the rear panel of the instrument.

For electrodes with a separate reference, connect the electrode’s BNC to the BNC connector and the 

electrode’s reference to the reference input socket.

For temperature measurement and automatic temperature compensation, connect the temperature 

probe to the appropriate socket (to desired channel - HI5222 only).

INSTRUMENT START UP

• Please ensure that the capacitive keypad is not covered by hand or other objects at the meter 

power on.

• Turn the instrument on from the power button located on the rear panel of the instrument.

• Please wait until the instrument finishes the initialization process.

Note: It is normal for the loading process to take a few seconds. If the instrument doesn’t 

display the next screen, restart the meter using the power button. If the problem persists, 

contact your dealer.

OPERATIONAL GUIDE
11

CHANNEL SELECTION (HI5222 only)

• Press while in Measure mode to access channel 

selection menu. 

Four available options will be displayed: Channel 

1, Channel 2, or multi-channel with the first or 

the second channel focused. The “Choose Channel 

Configuration” message is displayed in the Reminder 

messages area.

• Select the desired option by pressing the appropriate 

key: , , or .

The instrument will display in the selected option 

Measure mode.

OPERATIONAL GUIDE

For each measurement mode (pH, mV, Rel mV or ISE) the following display configurations are 

available: Basic, Good Laboratory Practice (GLP) (pH, ISE only), Graph and Log History.

Basic

The main measured value and it’s units are displayed on the LCD, along with the temperature 

value, temperature probe status and basic calibration information when this option is accessed.

To choose the Basic display mode:

• Press while in Measure mode. The “Choose Display Configuration” message will be 

displayed in the Reminder messages area.

• Press . The instrument will display the basic information for the selected measurement 

mode.

DISPLAYING MODES
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GLP

Detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only 

when this option is selected: Last Calibration date and time, Offset and Slope values, Calibration

Buffers/Standards and general information regarding the buffers/standards: the calibration

temperature, temperature compensation mode, date and time. For pH Measure, the Electrode 

Condition is also displayed on the LCD in percent.

Note: If a one point pH calibration is performed or the current calibration does not include at

least two consecutive standard buffers of pH 4.01, 7.01 (6.86) and 10.01 (9.18) buffers, the

Electrode Condition will be unknown. Electrode condition remains active for 24 hours after a 

calibration.

To access the GLP display option:

• Press while in Measure mode. The “Choose Display Configuration” message will be 

displayed in the Reminder messages area.

• Press . The instrument will display the detailed GLP data.

DISPLAYING MODES
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Graph

The on-line graph with currently logged values (pH, mV, Rel mV, or ISE vs. Seconds) will be 

displayed when this option is selected.

If there is no active log, the previously logged data for the selected parameter will be shown.

To access the off-line / on-line graph:

• Press while in Measure / Logging mode. The “Choose Display Configuration” message 

will be displayed in the Reminder messages area.

• Press . Press to begin an online graph.

To Zoom Graph

• Press then . and will appear in virtual keys.

• Use and to move along the X (Time) axis of the graph.

• Press to access the zoom menu for Y axis. Use or for zooming Y 

(parameter) axis.

• Press to return to the main menu.

When the off-line graph is displayed:

• Use the arrow keys to move along the X (Time) and Y (parameter) axes of the graph.

• Press to access the zoom menu for X and Y axes. Use or / /

 / to switch between the active zooming axes. Press or to 

zoom the selected axis.

Note: While in zoom graph menu the key is not accessible.

Press to return to the main menu.

DISPLAYING MODES
14

Log History

The measurement, along with Log History, will be visible when this option is selected:

1) The last stored logged data (Not actively logging) or

2) The last data logged from an active logging lot or

3) An empty display - NO LOTS saved, NOT currently logging

The log history list also contains the appropriate mV values, the logged temperature, the temperature 

probe status source, as well as the records time stamp.

To access the Log History display option:

• Press while in Measure mode. The “Choose Display Configuration” message will be 

displayed in the Reminder messages area.

• Press . The instrument will display the log history regarding the selected Measure

mode.

Notes: When an alarm condition is active, all logged records will have an exclamation mark “!”

If logged in Auto Hold, logged records will have an “H”.

If another Measure mode is selected, the Log History will reset.

If the temperature unit is changed, all logged temperature values will be

automatically displayed in the new temperature unit.

DISPLAYING MODES
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To access a System Setup option:

• Use or to highlight the desired 

option.

• Press to access the selected option.

The following is a detailed description of the System Setup option screen:

Beeper

This option allows the user to turn an acoustic warning 

signal on or off. This function can be used to signal 

4 different events: a stable signal, an alarm state, 

when every key is pressed or when an incorrect key 

is pressed. Enable (or disable) the Beeper for these 

events. Disabling the Beeper will stop audible signals.

The System Setup menu allows the user to customize the user interface, view meter information, 

set the external serial communication interface and to restore the manufacturer settings.

Accessing System Setup

• Press while in Measure mode.

• Press . The system setup options will be 

displayed on the LCD.

SYSTEM SETUP
16

Saving Confirmation

Enable this option to force confirmation of a change made 

to a setting in GLP data option field or a Sample ID

name. If Saving Confirmation is enabled, the user will 

have to accept the change with a key stroke. If Saving 

Confirmation is disabled, the changes made to these 

fields change automatically without asking for confirmation

GLP Data

Use this option to customize logging GLP information with 

specific identification data. When enabled, these ID tags will 

be included in the GLP section of all data logs for all modes of operation and both channels (HI5222). 

Each data field can use up to 10 characters. 

The five available fields are:

Operator ID : used to add the name of the operator

Instrument ID : used to name an instrument with a discrete name, location or number

Company Name : used to include the Company ID to the GLP data field.

Additional Info : two data fields are available for general notes or notations.

To add the GLP Data:

• Press while in Measure mode.

• Press .

• Use or to select the GLP Data option.

• Press and use or to highlight 

the desired option.

• Press to edit the desired information. The Text 

Editor menu will be displayed on the LCD.

• Enter the desired information by accepting the

highlighted character which is added to the text bar, 

using . The and keys help the 

user to select the desired character. It is also possible to delete the last character by positioning the 

cursor on the Backspace character ( ) and pressing .

SYSTEM SETUP
17

• Press to return to the GLP Data options. If the Saving Confirmation is enabled, press 

 to accept the modified option, to escape without saving or to return 

to the editing mode. Otherwise, the modified options are saved automatically.

Date & Time

Set the current date & time and the format in which they appear.

Set Date and Time

This option allows the user to set the current date (year/month/day) and time (hour/minute/second).

Notes: Only years starting with 2000 are accepted.

The time is set using the selected time format. For 12 Hour time format only, the AM/PM

can also be selected with or .

Set Time Format

Choose between 12-Hour (AM/PM) time format or 24-Hour time format.

Set Date Format

Choose the desired date format from 7 available options: DD/MM/YYYY, MM/DD/YYYY, YYYY/MM/DD, 

YYYY-MM-DD, Mon DD, YYYY, DD-Mon-YYYY or YYYY-Mon-DD.

To set the Date & Time:

• Press while in Measure mode.

• Press .

• Use or to select the Date & Time 

option.

• Press and use or to 

highlight the desired option you want to modify.

• Press to confirm your selection. Use

 / to select next/previous entry 

to be edited. Press and use or 

 to set the desired value, then press 

to save the modified value (for Set Date and Time

option). For the Set Date Format option press 

 to confirm your selection and select one of 

the displayed options with or .

• Press to confirm your selection and return to the Date & Time options or press 

to cancel operation.

SYSTEM SETUP
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Note: If the time is changed with more than one hour before last pH/ISE calibration, a 

pop-up warning will appear on the LCD, notifying the user that a date/time conflict has 

occurred and some time-dependent modes could work improperly (e.g. Measure, GLP, Log).

LCD Setup

This option allows the user to set the Contrast, the Backlight of the LCD and the Backlight Saver. The 

Contrast parameter can be adjusted within 7 steps, while the Backlight parameter within 8 steps. 

The Backlight Saver can be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are 

visible on the LCD for each parameter.

Note: If the instrument backlight turns off after the set period of time, press any key to turn 

it back on.

To set the LCD Setup:

• Press while in Measure mode.

• Press .

• Use or to select the LCD Setup option.

• Press and use key to highlight the desired 

parameter.

• Use or to adjust the contrast / backlight 

or to set the desired backlight saver time.

• Press to confirm the modified options and return 

to the System Setup menu.

SYSTEM SETUP

Color Palette

This option allow the user to choose desired color palette.

To select the Color Palette:

• Press while in Measure mode.

• Press .

• Use or to select the Color Palette

option.

Color 1 White background blue text

Color 2 Blue background white text

Color 3 White background black text

Color 4 Black background white text
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• Press and use or to highlight the desired color.

• Press to confirm your selection and return to the System Setup menu or press 

to return to the System Setup menu without changing.

Language

This option allows the user to choose the desired language in which all information will be displayed.

To select the Language:

• Press while in Measure mode.

• Press .

• Use or to select the Language

option.

• Press and use or to 

highlight the desired language.

• Press to confirm your selection and return to 

the System Setup menu or press to return to 

the System Setup menu without changing.

Serial Communication

This option allows the user to set the desired speed for the serial communication (baud rate) in bps. 

The meter and the PC program must have the same baud rate.

To set the Serial Communication:

• Press while in Measure mode.

• Press .

• Use or to select the Serial

Communication option.

• Press and use or to 

highlight the desired baud rate.

• Press to confirm your selection and return to 

the System Setup menu or press to return to 

the System Setup menu without changing.

SYSTEM SETUP
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Meter Information

This option provides general information about the instrument serial number (each instrument has a 

unique identification serial number), the software version and the factory calibration date and time 

(for mV and temperature).

Note: All instruments are factory calibrated for mV and temperature. One year after factory 

calibration, a warning message “Factory Calibration Expired” will be displayed when powering 

up the instrument. The instrument will still function, however, it should be taken to the nearest 

Hanna Customer Service for factory calibration. 

To view the Meter Information:

• Press while in Measure mode.

• Press .

• Use or to select the Meter

Information option.

• Press to acces the Meter Information menu.

• Press to return to the System Setup menu.

Restore Factory Settings

This option allows the user to erase all user settings and reset the instrument to the default factory 

settings in pH mode.

To restore the Factory Settings:

• Press while in Measure mode.

• Press .

• Use or to select the Restore Factory 

Settings option.

• Press to confirm your selection. A pop-up 

menu will be displayed, asking for confirmation.

• Press to confirm your selection and return to 

the System Setup or press to return to the 

System Setup menu without restoring defaults.

• Press to return to Measure mode.

SYSTEM SETUP
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Software update

This function allows the user to update instrument software. In order to start the PC upgrade application, you 

need to select the proper baud rate, the software update 

package and start the update.

The pH Setup menu allows the user to set the parameters associated with pH measurement and 

calibration. These parameters can be set specifically for each channel (HI5222 only). The settings 

will be applied only to the active channel.

Accessing pH Setup

• Press while in Measure mode and then 

 to select pH range for the desired channel.

• Press and then to access pH Setup

menu.

To access a pH Setup option:

• Use or to highlight the desired 

option.

• Press to access the selected option.

The following is a detailed description of the pH Setup 

option screens.

SYSTEM SETUP pH SETUP
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Profile

This option opens the Profile manager. Enabling Profile

allows the user to Save, Load or Delete an application 

Profile. The Profile option allows the user to store up 

to ten separate profile applications (HI5222 - five profiles 

for each channel). Each Profile can be named and recalled 

at a moment’s notice. A profile is a sensor setup complete 

with measurement units, logging and display preferences, 

calibration standards (Buffer or Standards including

custom), setup of the Display screen for measurement

(i.e. single, dual, graphing, GLP) and any other sensor 

configuration. Once saved, the exact same profile can be 

used at another time. This is a handy feature if the meter 

is used occasionally for additional applications because it 

saves time in the setup of the meter and ensures that the same procedure will be used.

To save the measurement configuration for pH mode:

• Press and use or to highlight Profile.

• Press / to enable / disable this feature.

The available options are:

Save Profile: save the current profile.

Save Profile As...: save current profile using a specific name.

Load Profile: load from available profiles.

Delete Profile: delete a profile.

Save Current Profile

To save the current profile:

• Use or to select Save Profile As...

• Press . The Text Editor box will be displayed on the LCD.

• Enter the desired profile name by using and to highlight the desired character 

and then press to add it to the text bar. It is also possible to delete the last character by 

positioning the cursor on the Backspace character( )and pressing .

• Press to return to the Profile options.

• Use Save Profile to save changes made to a presently used Profile. Changes will overwrite 

existing configurations.

pH SETUP
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• Select Load Profile to select a profile to use from the list of saved profiles. Highlight the desired 

profile and press .

• Select Delete Profile to remove a selected profile from the saved list.

Highlight the profile and press .

Temperature

The temperature has a direct influence on pH. This option allows the user to choose the temperature 

source and units, as well as the desired manual temperature for manual temperature compensation 

mode.

Temperature Source (HI5222 only)

If using a temperature probe, Automatic Temperature Compensation will be performed relative to the 

displayed temperature, with the “ATC” indicator displayed on the LCD. For the HI5222, one temperature 

probe can be used for both measurement channels if desired. Select the source by selecting Manual, 

Channel 1 or Channel 2 . If no temperature probe is detected, Manual Temperature Compensation 

will be performed, with the “MTC” indicator on the LCD.

Temperature Unit

The desired temperature unit can be chosen (Celsius, Fahrenheit or Kelvin degrees) and the meter will 

automatically make the conversion for the selected unit.

If no temperature probe is connected, the desired temperature can be set manually. The default setting 

is 25.0 °C. If the measured temperature is different, the value can be manually adjusted to obtain 

an accurate pH measurement.

To set one of the Temperature options:

• Press while in pH Measure mode.

• Press .

• Use or to select the Temperature 

option.

• Press and use or to highlight 

the desired Temperature option you wish to modify.

• Press and use or to highlight 

the desired option (for Temperature Source & Unit 

options) or use or to adjust the 

temperature value between the displayed limits (for 

Manual Temperature option).

pH SETUP
24

• Press to confirm your selection (for Temperature Source & Unit options) or press 

to save the current value (for Manual Temperature option). Otherwise, press to cancel 

operation.

Calibration

This option allows the user to setup desired buffers and calibration reminder.

Buffer Entry Type

Three settings are available for the pH buffers used for electrode calibration:

Automatic - the instrument automatically selects the closest buffer to the measured pH value from 

the predefined buffers chosen in the option Edit Buffer Group.

Semiautomatic - the instrument automatically selects the closest buffers to the measured pH value 

from all available buffers and you can choose the one used to be, from standard and custom buffers.

Manual Selection - the desired pH buffer is manually selected from all available buffers (standard 

and custom).

To set the Buffer Entry Type:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the Buffer Entry Type option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

to cancel operation.

1st Cal. Point

Two options are available for the 1st Cal. Point parameter: Point and Offset.

Point: A new buffer can be added to an existing calibration. The electrode slope will be reevaluated 

with the addition of this buffer (normal operation).

Offset: The new buffer calibration point can create a constant offset to all existing pH calibration 

data (existing calibration must have a minimum of two pH buffers). 

pH SETUP
25

Edit Custom Buffers

If special custom pH buffers are required during calibration, the Edit Custom Buffers option is available. Up to 

five pH custom buffers can be added. If a custom buffer 

is used, the user must verify it’s value at temperature 

of calibration.

To edit/set the Custom Buffers:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the Edit Custom Buffers option.

• For a previous set value, press to set the custom buffer value to “----” if desired and 

confirm the setting by pressing , otherwise press to edit the selected custom 

buffer.

• While in edit custom buffer menu press to set the custom buffer value to 7.000 pH and 

then use or to set the desired custom buffer value.

To set the 1st Cal. Point:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the 1st Cal. Point option.

• Press / as desired.

• Press to return to previous menu.

pH SETUP
26

• Press to exit custom buffer edit menu. If the Saving Confirmation is enabled, press 

 to accept the modified option, to escape without saving or to return 

to the editing mode. Otherwise, the modified option is saved automatically.

• Use key to select the next custom buffer to be set or press to return to Calibration 

options.

Edit Buffer Group

Accessing this option, the user can edit the desired group of 

five pH buffers for automatic buffer recognition (Automatic 

Buffer Entry Type). If the Buffer Group already contains five 

pH buffers, at least one pH buffer has to be removed in order 

to add another buffer.

To edit/set the Buffer Group:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration option.

• Press and use or to highlight 

the Edit Buffer Group option.

• Press and use and to choose the pH buffer to be included in the buffer 

group.

• Press or to add/remove the selected pH buffer to/from the buffer group.

• Press to return to Calibration options and to save the changes.

Calibration Reminder

This option allows the user to select a calibration reminder 

schedule if desired. Three options are available for the

calibration reminder: Daily, Periodic or Disabled.

To set the Calibration Reminder:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration option.

pH SETUP
27

Set Reminder Period

Schedule the calibration reminder timing with this option 

(verify Daily or Periodic is set for Calibration Reminder).

If a Daily reminder is desired, set the time of day you 

wish the reminder to occur.

If a Periodic reminder is desired, schedule time in days, 

hours and/or minutes after the last calibration for the 

reminder to occur. 

To set the Reminder Period:

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to highlight the Set Reminder Period option.

• Press and use / to select next/previous entry to be edited.

• Press and use or to set the desired value, then press to save 

the modified value.

• Press to return to the Calibration options. If the Saving Confirmation is enabled, press 

 to accept the modified option, to escape without saving or to return 

to the editing mode. Otherwise, the modified option is saved automatically.

Clear Calibration

This feature deletes the pH electrode calibration for the attached electrode and channel (HI5222). A 

default meter calibration will replace the actual electrode calibration until a new electrode calibration 

is made.

• Press and use or to highlight the Calibration Reminder option.

• Press and use or to highlight the desired option.

• Press to confirm your selection or press to cancel operation.

pH SETUP
28

• Press while in pH Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the Clear Calibration option.

• Press to clear calibration. A pop-up menu 

will be displayed asking for confirmation (when a 

calibration is available).

• Press to confirm or press to escape 

without saving and return to the Calibration options.

Sample ID

This option allows the user to assign an identification 

number/name. Two Sample ID options are available:

ID Increment and Edit Sample ID.

ID Increment 

Two choices are available for the sample ID:

None - the sample ID will be fixed and it can be set 

alphanumeric (see Edit Sample ID).

Automatic -the sample ID will automatically increment 

by one for each new log lot.

To set the ID Increment mode:

• Press while in pH Measure mode.

• Press .

• Use or to select the Sample ID option.

• Press and use or to highlight the ID Increment option.

• Press / as desired.

• Press to return to previous menu.

pH SETUP

To clear Calibration:
29

Edit Sample ID

This option allows the user to edit the sample ID.

Note: The ID Increment mode must be set to None, 

to use this feature.

To edit the Sample ID:

• Press while in pH Measure mode.

• Press .

• Use or to select the Sample ID 

option.

• Press and use or to 

highlight the Edit Sample ID option.

• Press to confirm your selection.

• If the selected increment is None, the Text Editor menu will be displayed on the LCD, allowing you 

to enter the desired sample number/name by accepting the highlighted character which is added 

in the text bar, using . The and keys help the user to select the desired 

character.

It is also possible to delete the last character; position the cursor on the Backspace character and 

press .

• Press to return to Sample ID options. If the

Saving Confirmation is enabled, press 

to accept the modified option, to escape 

without saving or to return to the editing 

mode.

Otherwise, the modified options are saved

automatically.

• If the selected increment mode is Automatic, the desired 

sample ID value can be set using or 

• Press to save the current value or press 

to cancel operation.

pH SETUP
30

Stability Criteria

This option allows the user to select the signal stability criterion for the measured parameter (pH, 

mV, ISE):

Fast - this setting will give faster results with less accuracy.

Medium - this setting will give medium speed results with medium accuracy.

Accurate - this setting will give slower results with high accuracy.

To set the Stability Criteria:

• Press while in pH Measure mode.

• Press .

• Use or to select the Stability 

Criteria option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

to cancel operation.

Reading Mode

This option allows the user to select between Direct and 

Direct/AutoHold pH reading mode. If choosing the AutoHold option, the current reading can be frozen 

on the LCD when is pressed and the stability criterion is reached.

To set the Reading Mode:

• Press while in pH Measure mode.

• Press .

• Use or to select the Reading Mode

option.

• Press / to select Direct / AutoHold 

option as desired.

• Press to cancel operation.

pH SETUP
31

Log

Note: See section Logging for available types of logging.

This option allows the user to edit the logging settings: Logging Type, Logging Data Configuration, 

Sampling Period and New Lot.

Logging Type

Three logging types are available: Automatic, Manual and Auto Hold. 

Automatic - the measurement data is logged automatically at predefined time intervals;

Manual - a snapshot of the displayed measurement data is logged with time stamp when the user 

manually depresses Log;

Auto Hold - this is configured along with the Direct/AutoHold Reading Mode to take a snapshot of 

stable measurement data. Press to initiate a logging session. Press to initiate an 

Auto Hold event. The log occurs automatically once measurement stability is reached. This type log 

removes subjective data, as it only captures stable measurements.

To set the Logging Type:

• Press while in pH Measure mode.

• Press .

• Use or to select the Log option.

• Press and use or to 

highlight the Logging Type option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

 to cancel operation.

Logging Data Configuration

This option allows the user to select which additional parameters will accompany a log report: 

Date/Time, Calibration Data, Sample ID, Instrument ID, Operator ID, Company Name, Additional 

Info 1 and Additional Info 2.

pH SETUP
32

To set the Logging Data Configuration:

• Press while in pH Measure mode.

• Press .

• Use or to select the Log option.

• Press and use or to highlight 

the Logging Data Configuration option.

• Press and use or to highlight 

the desired parameter to be logged in file.

• Press to enable the parameter or to 

disable it.

• Press to return to previous menu.

pH SETUP

Sampling Period

This option allows the user to select the desired sampling period 

for automatic logging type.

To set the Sampling Period:

• Press while in pH Measure mode.

• Press .

• Use or to select the Log option.

• Press and use or to highlight 

the Sampling Period option.

• Press and use or to select the 

desired option.

• Press to confirm your selection or press to cancel operation.

New Lot

This option is used to create a new lot when manual logging is used.

Note: If New Lot option is accessed and the Logging Type is Automatic, a warning message 

appears on the LCD informing the user that a new lot can be created only if the Logging Type 

is set as Manual.
33

pH SETUP

To generate a New Lot:

• Press while in pH Measure mode.

• Press .

• Use or to select the Log option.

• Press and use or to highlight the 

New Lot option.

• Press to generate a new manual lot. A pop-up 

menu will be displayed asking for confirmation.

• Press to confirm or press to escape 

without saving and return to the Log options.

Alarm

This option allows the user to select the alarm settings: Alarm State and Alarm Limits. If the Alarm 

option is enabled, a continuous double beep will be heard, along with the “Alarm” indicator blinking 

on the LCD, each time the set limits in Measure mode are exceeded.

Note: Alarm Beeper must be set On for audible beep to be heard.

See: System Setup > Beeper > Alarm.

Alarm State

Three settings are available for the Alarm State option:

Disabled - the alarm will be disabled.

Inside Limits - the alarm state will trigger when the 

measured value is inside the set limits.

Outside Limits - the alarm state will trigger when the 

measured value is outside the set limits.

To set the Alarm State:

• Press while in pH Measure mode.

• Press .

• Use or to select the Alarm option.

• Press and use or to 

highlight the Alarm State option.

• Press and use or to highlight the desired option.

• Press to confirm your selection or press to cancel operation.

Alarm Limits

This option allows the user to set the alarm limits for the measured value.

Note:The Alarm High value can not be lower than the Alarm Low value.
34

To set the Alarm Limits:

• Press while in pH Measure mode.

• Press .

• Use or to select the Alarm option.

• Press and use or to 

highlight the Alarm Limits option.

• Press and use / to select 

next/previous entry to be edited.

• Press and use or to set the 

desired value, then press to save the modified 

value.

• Press to return to the Alarm options. The 

modified option is saved automatically.

Isopotential Point

This option allows the user to edit the isopotential point of the electrode used for pH measurements. 

The isopotential point is the mV reading for an electrode at which temperature has no effect on the 

measurement. The ideal electrode has an isopotential point of 0.0 mV and 7.00 pH, while an actual 

electrode typically deviates slightly from the ideal values.

If the actual isopotential pH for an electrode is known, it can be set by accessing this option.

Note: If the isopotential point has been modified, recalibration must be performed.

To set the Isopotential Point:

• Press while in pH Measure mode.

• Press .

• Use or to select the Isopotential 

Point option.

• Press and set the desired isopotential pH value 

using or .

• Press to save the current value or press 

to cancel operation.

pH SETUP
35

The mV Setup menu allows the user to set the parameters associated with mV and Relative mV 

measurements. These parameters can be set specifically for each channel (HI5222 only). The settings will be applied only to the active channel.

Accessing mV Setup

• Press while in Measure mode and then or to select mV / Rel mV 

range for the desired channel.

• Press and then to access mV Setup 

menu.

To access a mV Setup option:

• Use or to highlight the desired 

option.

• Press to access the selected option.

The following is a detailed description of the mV Setup 

option screens.

pH SETUP

pH Resolution

Select the desired pH resolution with this option. Choose 

from one (X.X), two (X.XX) or three (X.XXX) digits

displayed past the decimals.

To set the pH Resolution:

• Press while in pH Measure mode.

• Press .

• Use or to select the pH Resolution

option.

• Press and use or to highlight 

the desired option.

• Press to confirm your selection or press to cancel operation.

mV SETUP
36

Profile- See pH Setup section.

Temperature

ORP measurements are not temperature compensated, 

although ORP values can change with temperature

(e.g. reference electrode potential changes, sample 

equilibrium changes). It is important to report ORP 

values together with the reference electrode used and the

temperature of measurement.

This option permits selection of the temperature source

(HI5222 only) and measurement units.

Temperature Source

If using a temperature probe, sample temperature will be displayed with the “ATC” indicator displayed 

on the LCD. For HI5222 only, the ATC option can be selected from Channel 1 or Channel 2. If no

temperature probe is detected, a Manual set value will be displayed (and logged) with the measurement.

Temperature Unit

Select the desired temperature unit (Celsius, Fahrenheit or Kelvin degrees) and the meter will

automatically convert to the selected unit.

Manual Temperature

If no temperature probe is connected, the desired temperature can be manually entered. The 

default setting is 25.0 °C.

Calibration (Relative mV only)

Calibration Reminder

This option allows the user to select a calibration reminder schedule if desired.

See pH Setup section > Calibration Reminder section for option access details.

Set Reminder Period

See pH Setup section > Set Reminder Period section.

mV SETUP
37

This feature deletes the Relative mV calibration for the selected channel.

• Press while in Rel mV mode.

• Press then use or to access Calibration option.

• Press and use or to highlight Clear Calibration option.

• Press to clear calibration. A pop-up menu will be displayed asking for confirmation 

(when a calibration is available).

• Press to confirm or press to escape without saving and return to the Calibration 

options.

Sample ID - See pH Setup section.

Stability Criteria - See pH Setup section.

Reading Mode - See pH Setup section.

Log - See Logging section, or pH Setup section.

Alarm - See pH Setup section.

mV SETUP ISE SETUP (HI5222 only)

The ISE Setup menu allows the user to set the parameters regarding ISE measurement and 

calibration. These parameters can be set specifically for each channel. The settings will be applied 

only to the active channel.

Accessing ISE Setup

• Press while in Measure mode and then 

 to select ISE range for the desired channel.

• Press and then to access ISE Setup 

menu.

To access an ISE Setup option:

• Use or to highlight the desired 

option.

• Press to access the selected option.

The following is a detailed description of the ISE Setup 

option screens.

Clear Calibration
38

Profile - See pH Setup section.

Reading Mode

This option allows the user to select the desired reading mode: Direct, Direct/AutoHold, Known Addition, Known Subtraction, Analyte Addition and Analyte Subtraction. Four of these Reading Modes are 

collectively known as Incremental Methods (see ISE Theory section for details). Direct measurements 

and Direct/AutoHold measurements are also available.

Direct

Direct measurements are analogous to taking pH measurements. The ISE is calibrated in ion standards 

and sample measurements are made directly. The ISE’s manual should be consulted for tips and practices of making Direct measurements. The ion concentration can be read directly from the instrument.

Direct/AutoHold

Direct/AutoHold measurements are made similar to Direct measurements. The advantage of using 

Direct/AutoHold is a measurement that has not reached equilibrium will not be used. Only after 

the chosen stability criteria has been met will the meter go into the Direct/AutoHold mode. Using 

Direct/AutoHold removes the subjective nature of stability.

Known Addition

In the Known Addition method, a sample is measured with an ISE before and after the addition of 

a known volume of a standard. The mV difference is then used to calculate the concentration of the 

ion in the original sample.

Known Subtraction

In the Known Subtraction method, a sample is measured with an ISE before and after the addition 

of a known volume of a reactant standard. The reactant standard reacts with the measured ion in 

the sample, reducing it’s concentration. The mV difference is then used to calculate the concentration 

of the ion in the original sample. The stoichiometric ratio between reactant standard and ion in the 

sample must be known.

Analyte Addition

Analyte Addition is similar to the Known Addition method, with the difference being that an aliquot 

of sample is added to a known volume of standard. Both solutions contain the same measured ion. The 

standard is measured with an ISE before and after the addition of a known volume of a sample. The 

ion concentration is then calculated using the difference in mV potential. The sample should increase 

the concentration of the ion being measured.

ISE SETUP (HI5222 only)
39

Analyte Subtraction

In the Analyte Subtraction method, an aliquot of sample is added to a reactant standard of known 

concentration and volume. The sample partially reacts with the measured ion. The stoichiometric ratio 

between standard and sample must be known. The ion concentration is then calculated using the 

difference in mV potential.

To set the Reading Mode:

• Press while in ISE Measure mode.

• Press .

• Use or to select the Reading Mode 

option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

to cancel operation.

Temperature

This option permits the user to configure all parameters 

related to ISE temperature measurements.

Temperature Source

The options are: Manual, Channel 1 or Channel 2. If no 

temperature probe is detected, a manually set value will 

be displayed (and logged) with the measurement. If a 

temperature probe is connected to either channel, it may be 

selected. The temperature measurement will be displayed 

and logged with the measurement and may be used for 

temperature compensation calculation if Temperature 

Compensation is enabled.

Temperature Unit

Select the desired temperature unit (Celsius, Fahrenheit or Kelvin degrees) and the meter will automatically convert to the selected unit.

Manual Temperature

If no temperature probe is connected, the desired temperature can be set manually. The default setting 

is 25.0 °C. If the measured temperature is different, the value can be manually adjusted to obtain 

an accurate ion measurement.

ISE SETUP (HI5222 only)
40

Temperature Compensation

ISE measurements benefit from temperature compensated corrections if:

• standards and sample temperatures differ from each other

• the Isopotential Point of the ISE is known. 

If sample and standards are made at the same temperature, leave this option disabled.

Isopotential Point

If the Temperature Compensation is enabled, the isopotential point of the ISE must be added in this parameter. Verify 

the Electrode Type and Concentration Unit are configured 

for the desired application. The Isopotential point will use 

the selected concentration unit. Use and 

to edit the isopotential point value and press to 

save the value or press to cancel operation.

Notes: A warning message will appear on the LCD 

informing the user to perform a new calibration.

A minimum of two ion standards is required for the 

ISE calibration.

Calibration

This option allows the user to view and configure all ISE parameters related to ISE calibration.

Manual Entry

Two different standard groups can be used for calibration of ISE:

All Standards - During calibration the user can select 

the desired standards from a large list containing all the 

predefined standard values and the custom standards.

Group Standards - the user can pre-select a group of 

standards from the existent group of standards to be used 

during sensor calibration.

ISE SETUP (HI5222 only)
41

• Press while in ISE Measure mode.

• Press .

• Use or to select the Calibration option.

• Press and use or to highlight the Manual Entry option.

• Press or to select the desired option.

Edit Custom Standards

Use Edit Custom Standards function to add additional 

ISE standard values. Up to five custom standard values 

can be added. Set Electrode Type and Concentration Unit

prior to adding these standards.

To edit/set the Custom Standards:

• Press while in ISE Measure mode.

• Press .

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the Edit Custom Standards option.

• If you want to disable the custom standard, press . A pop-up menu will be displayed 

asking for confirmation. Press to confirm (the custom standard value will turn to “----”) 

or press to cancel the operation.

• Use key to select the next custom standard to be set.

• Press to return to Edit Custom Standard options.

ISE SETUP (HI5222 only)

Edit Standard Group

If a Group Standard was selected in the parameter Manual Entry, this parameter is used to create 

your group of standards. If the Standard Group already contains five ISE standards, at least one 

ISE standard has to be removed in order to add another standard.

To edit/set the Standard Group:

• Press while in ISE Measure mode.

• Press .

To set the Manual Entry:
42

• Use or to select the Calibration 

option.

• Press and use or to 

highlight the Edit Standard Group option.

• Press and use and to 

choose the ISE standard to be included in the standard 

group.

• Press / to add/remove the selected 

ISE standard to/from the standard group.

• Press to return to Calibration options and to 

save the changes.

Calibration Reminder - See Calibration option from 

pH Setup section.

Set Reminder Period - See Calibration option from pH Setup section.

Clear Calibration - See Calibration option from pH Setup section.

Electrode Type

This option allows the user to select the desired Ion Selective Electrode used for measurements from 

a list: Ammonia, Bromide, Cadmium, Calcium, Carbon Dioxide, Chloride, Cupric, Cyanide, Fluoride, 

Iodide, Lead, Nitrate, Potassium, Silver, Sodium, Sulfate, Sulfide and five custom ISE. For the standard 

ISE it is possible to view the Ion constants (Name, Molar Weight and Electric Charge/Slope), while for 

the custom ISE all these constants can be manually set.

To set the Electrode Type:

• Press while in ISE Measure mode.

• Press .

• Use or to select the Electrode Type 

option.

• Press and use or to select 

the desired standard ISE or a custom one from the list.

For standard ISE:

• Press to visualize the Ion Constants and then 

press at any time to exit Ion Constants view 

mode.

ISE SETUP (HI5222 only)
43

• Press to confirm your selection and return to ISE Setup options.

For custom ISE:

• Press to edit the Ion Constants for the selected 

custom ISE. Use or to select the 

desired constant and press to enter edit mode 

or to cancel operation.

• For the ion Name the Text Editor menu will be

displayed on the LCD. Enter the desired information by 

accepting the highlighted character which is added in 

the text bar, using . The and 

keys help the user to select the desired character. It is 

also possible to delete the last character by positioning 

the cursor on the Backspace character ( ) and 

pressing . Press to return to the 

Ion Constants menu.If the Saving Confirmation is 

enabled, press to accept the modified option, 

 to escape without saving or to return 

to the editing mode. Otherwise, the modified option is 

saved automatically.

• To set the appropriate Ion Molar Weight (in g/mol units) 

use or to set the desired value and 

then press to save the current value or press 

to cancel operation.

• To select the appropriateIon Charge/Slope use 

or and then press . If the ion electric 

charge is None, its slope can be manually set by 

pressing .

A pop-up menu will be displayed on the LCD, in which the slope value can be set using 

or . Press to save the modified value or press to return to the previous 

menu.

ISE SETUP (HI5222 only)
44

Note: If an ISE calibration was performed and a 

different Ion Selective Electrode is selected (standard 

or custom), a warning message appears on the LCD 

informing the user to perform a new calibration or to 

select the previous ISE in order to perform accurate 

measurements.

Concentration Unit

Select the desired concentration unit for the measured ion or chemical compound. The available 

concentration units are: ppt, g/L, ppm, mg/L, μg/mL, ppb, μg/L, mg/mL, M, mol/L, mmol/L, 

%w/v and User (custom unit).

To set the Concentration Unit:

• Press while in ISE Measure mode.

• Press .

• Use or to select the Concentration 

Unit option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

to cancel operation.

Sample ID - See pH Setup section.

Stability Criteria - See pH Setup section.

Log - See pH Setup section.

Note: The Logging Data Configuration option includes also the Ion Constants parameter. If you 

want it to appear in the log reports, it must be enabled.

Alarm - See pH Setup section.

Note: The Alarm Limits (Low and High) are set in the selected concentration unit of the measured 

ISE SETUP (HI5222 only)
45

ISE Significant Digits

Accessing this option, the number of ISE significant 

digits can be set, with one (X), two (XX) or three (XXX) 

significant digits. 

To set the ISE Significant Digits:

• Press while in ISE Measure mode.

• Press .

• Use or to select the ISE Significant 

Digits option.

• Press and use or to 

highlight the desired option.

• Press to confirm your selection or press 

 to cancel operation.

ISE SETUP (HI5222 only)

Calibrate the instrument often, especially if high accuracy is required. 

The instrument should be recalibrated:

• Whenever the pH electrode is replaced.

• At least once a week.

• After testing aggressive chemicals.

• When “Electrode Cond. Unknown”, “pH x Default Calibration” or “pH x Calibration Expired” 

message appears on the LCD, in the Reminder messages area. The “x” represents channel “1” or 

channel ”2”.

PREPARATION

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to 

minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each buffer 

solution. One for rinsing the electrode and one for calibration.

pH CALIBRATION
46

If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and pH 4.01, 3.00 

or 1.68 as second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as first 

buffer and pH 10.01/9.18 or 12.45 as second buffer.

For extended range measurements (acidic and alkaline), perform a five points calibration by selecting 

five of the available buffers.

pH CALIBRATION

CALIBRATION PROCEDURE

There are 8 standard pH buffers that are temperature-compensated during pH calibrations:

pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45. The standard pH buffers are temperature 

compensated during calibration. Custom buffers require the user to use the actual buffer value at 

the temperature of use.

A minimum of a two point calibration using the standard pH buffers is required to determine the 

pH electrode condition. The buffers should bracket the sample measurement pH.

An extended pH measurement range will require calibration at multiple points. The meter is able 

of calibration with 5 pH buffers. For improved measurement accuracy, perform a multiple buffer 

calibration bracketing and including the pH range the sample measurements. 

The buffer group that will be available during calibration was set in pH setup → Calibration Buffer 

Entry Type. The following example demonstrates pH electrode calibration if Manual selection was 

selected. In this case all of the 8 standard buffers will be available for calibration.

pH Calibration screen description

• Press . If the instrument was calibrated before and calibration was not cleared, the old 

calibration can be cleared by pressing . After 10 seconds, will no longer be 

available.

Note: It is very important to clear calibration history when a new electrode is used because 

most errors and warning messages that appear during calibration depend on calibration 

history.
47

• Immerse the pH electrode and the temperature probe approximately 4 cm (1.5”) into a buffer 

solution of your choice (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 or a custom 

buffer) and stir gently. The temperature probe should be close to the pH electrode.

• Select the pH calibration buffer used with or . The “Please 

wait...” message will appear on the LCD until the reading is stable or the 

buffer is validated.

• If the pH buffer is validated, will appear on the LCD. Press 

to update calibration. The calibration buffer will be added to the Calibrated 

Buffers section.

• Immerse the pH electrode and the temperature probe into the next buffer solution and follow the 

above procedure or press to exit calibration.

Notes: The new added calibration point will replace 

an old one if the difference between them is

±0.2 pH.

If the existing stored calibration is full (five calibration 

points), a pop-up menu will be displayed on the LCD 

in which you can select with or 

keys the buffer you want to replace with the current 

buffer. Press to delete the selected buffer 

and then press to update calibration with 

the new buffer.

pH CALIBRATION

• If using manual temperature, after selecting the standard buffer, press . A pop-up menu 

will be displayed on the LCD in which the temperature 

value can be adjusted using or . 

Press to save the new temperature value.

• If using Custom buffers, press after buffer 

has been accepted to change actual buffer conditions. 

A pop-up menu will be displayed on the LCD in which 

the custom buffer and the temperature value (MTC) 

can be adjusted by pressing and then 

 or keys. Press to save 

the modified value and then / to 

select next/previous value to be adjusted.
48

CALIBRATION MESSAGES

• Move sensor to next buffer or check buffer: this message appears when the difference 

between the pH reading and the value of the selected calibration buffer is significant. If this 

message is displayed, check if you have selected the appropriate calibration buffer.

• Wrong buffer temperature: this message appears if the buffer temperature is out of the 

defined buffer temperature range.

• Clean the electrode or check the buffer. Press to update calibration: this message alerts the user that some dirt or deposits could be on the electrode. Refer to the electrode 

Cleaning Procedure.

• Slope too low. Please check the buffer / Slope too high. Please check the buffer: these 

messages appear if the current slope is under 80 % or over 110 % of default slope. Recalibrate 

the instrument using fresh buffers.

• Slope too low. Press to clear old calibration / Slope too high. Press to 

clear old calibration: verify the correct buffer has been selected and poured.

• If the Automatic buffer entry type has been selected for the calibration procedure, the instr ment 

will automatically select the closest buffer to the measured pH value from the edit buffer group 

(see pH Setup for details).

• If the Semiautomatic buffer entry type has been selected for the calibration procedure, the 

instrument will display only the closest buffers to the measured pH value from all the available 

buffers and the user must select with or the buffer being used

• Unrecognized buffer. Please check the buffer or the buffer list (for Semiautomatic and 

Automatic buffer entry type): this message appears if the current buffer value is not close to 

any of the buffers from the buffer list/group. Check if the current buffer is present in the buffer 

list or the appropriate buffer group was selected.

• The current buffer was already calibrated: change the buffer or press to exit 

calibration mode.

pH CALIBRATION
49

pH MEASUREMENT

DIRECT MEASUREMENT

To measure the pH of a sample using the Direct reading 

mode:

• Press and then to select pH Measure mode (select channel first for HI5222).

• Select the Direct reading mode (see pH Setup for 

details).

• Place the electrode tip and the temperature probe 

approximately 4 cm (1.5”) into the sample to be 

tested. Allow time for the electrode to stabilize. 

“Stable” will appear.

• The measured pH value will be displayed on the LCD, 

together with a short GLP information and display preferences.

Note: If the reading is out of range, “----” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure pH of a sample using the Direct/AutoHold 

reading mode:

• Press and then to select pH Measure mode (for the selected channel - HI5222 only).

• Select the Direct/AutoHold reading mode (see pH 

Setup for details).

• Place the electrode tip and the temperature probe 

approximately 4 cm (1.5”) into the sample to be 

tested.

• The measured pH value will be displayed on the LCD. 

Press and the “AutoHold” indicator will 

start blinking on the LCD until the stability criterion 

is reached. The pH value will be frozen on the LCD, along with “AutoHold” indicator.

• To return to normal Measure mode press .

Note: If the reading is out of range, “----” will be displayed on the LCD.

Verify the pH electrode and instrument has been calibrated before making pH measurements.
50

Outside Cal Range warns the user if the current reading is out of the calibrated area. 

The calibrated area is that part of the pH range in which the calibration point ensures an

accurate reading. If the reading is taken out of the calibration area, the “Outside Cal 

Range”message will start blinking on the LCD. The 

calibrated area is calculated in accordance with the pH

resolution used during the measurement. To avoid triggering 

this message, the buffer values have to be well-distributed 

in the desired measurement range.

If measurements are taken successively in different samples, 

it is recommended to rinse the electrode thoroughly with 

deionized water or tap water and then with some of the next 

sample before immersing it into the next sample solution.

The pH reading is affected by temperature. In order to 

measure the pH accurately, the temperature effect must be 

compensated. To use the Automatic Temperature Compensation (ATC) feature, connect and place 

the HI7662-W temperature probe into the sample as close as possible to the electrode and wait 

for a few seconds.

If the temperature of the sample is known, Manual Temperature Compensation (MTC) can be used 

by disconnecting the temperature probe.

Notes: For single channel pH measurements (HI5221), “MTC” or “ATC” indicators will be 

displayed on the LCD, while for dual channel pH measurements (HI5222) “MTC1”/”MTC2” 

or “ATC1”/”ATC2” indicators will be displayed (Channel dependent).

For mV/Rel mV measurements, “NoProbe” or “TEMP” indicators will be displayed on the LCD 

(HI5221 only), respectively, “NoProbe 1”/“NoProbe 2” or “TEMP1”/”TEMP2” indicators

(HI5222 only) for mV/Rel mV/ISE measurements, depending on the temperature probe 

status and channel selected.

When in MTC mode, the temperature can be modified by pressing for pH Measure 

mode and for mV /Rel mV Measure mode (HI5221 only) if the Reading Mode option 

is Direct (HI5222 only). The temperature value can be adjusted with or from 

-20.0 °C to 120.0 °C. Press to save the new temperature value or press 

to return to Measure mode.

When in ATC mode for pH, or TEMP for mV/Rel mV, “----” will be displayed on the LCD if the 

measured temperature is under or over temperature range (-20.0 °C to 120.0 °C).

pH MEASUREMENT
51

mV & Relative mV MEASUREMENTS

mV/ORP MEASUREMENTS

Oxidation-reduction potential (ORP) measurements provide the quantification of the oxidizing or 

reducing power of the tested sample.

To correctly perform a redox measurement, the surface of the ORP electrode must be clean and 

smooth.

DIRECT MEASUREMENT

To measure the mV of a sample using the Direct reading 

mode:

• Press and then to enter mV Measure

mode (Select channel first for - HI5222 only).

• Select the Direct reading mode (see mV Setup for details).

• Place the tip of the ORP electrode 4 cm (1.5”) into the 

sample to be tested and allow a few seconds for the 

reading to stabilize.

• The instrument will display the measured mV value on 

 the LCD.

Note: If the reading is out of range, “----” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure mV of a sample using the Direct/AutoHold 

reading mode:

• Press and then to select mV Measure 

mode (Select channel first for - HI5222 only).

• Select the Direct/AutoHold reading mode (see mV Setup 

for details).

• Place the tip of the ORP electrode approximately 4 cm 

(1.5”) into the sample to be tested.

• The measured mV value will be displayed on the LCD. 

Press and the “AutoHold” indicator will start 

blinking on the LCD until the stability criterion is reached. The mV value will be frozen on the 

LCD, along with “AutoHold” indicator.

• To return to normal Measure mode press .

Note: If the reading is out of range, “----” will be displayed on the LCD.
52

Relative mV MEASUREMENTS

To measure the Relative mV of a sample:

• Press then (select channel first for 

HI5222).

• Verify if a current calibration has been made.

• If required, conduct the single point Rel mV calibration. 

Verify the tip of the electrode is immersed into the 

known solution or ORP standard.

• Press . Use and keys 

set the standard value. Press to store the 

calibration.

• Press then (select channel first for 

HI5222). 

• Place calibrated sensor tip into the sample to be 

analyzed. The instrument will display the measured 

Relative mV value on the LCD, together with a short 

GLP information about the last calibration or Offset: 

0.0 mV no Rel mV calibration was performed.

Notes: If the ORP sensor is not in solution or the 

measured mV potential is out of range, “----” will be 

displayed on the LCD.

mV & Relative mV MEASUREMENTS
53

For greater accuracy, it is recommended to calibrate the ISE sensors frequently. The instrument 

should be recalibrated when “ISE x Calibration Expired” (the “x” represents channel “1” or channel 

”2”) message appears on the LCD, in the Reminder messages area.

Due to electrode conditioning time, the electrode must be kept immersed a few seconds to stabilize. 

The user will be guided step by step during calibration with easy-to-follow messages on the display. 

This will make the calibration a simple and error-free procedure.

PREPARATION

Pour small quantities of the standard solutions into clean beakers. If possible, use plastic beakers 

to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each standard 

solution. One for rinsing the electrode and one for calibration.

Note: To read concentration (not activity) ISA must be added to the standards and samples. 

No corrections are needed due to dilutions.

CALIBRATION PROCEDURE

The ISE calibration and measurement can be performed with or without temperature compensation. 

If the temperature compensation option is enabled, the isopotential point of the electrode must be 

set in ISE Setup in order to perform correct concentration measurements.

Before calibrating, make sure that the appropriate Electrode Type has been selected in ISE Setup 

according to the measured Ion/compound.

ISE Calibration screen description

ISE CALIBRATION (HI5222 only)
54

The group of calibration standards is set under ISE Setup → Calibration. Select standards that are 

in the measurement range of the samples.

To calibrate the instrument:

• Press . If the instrument was calibrated before and calibration was not cleared, the old 

calibration can be cleared by pressing . After 10 seconds, will no longer be 

available.

Note: It is very important to clear calibration history when a new electrode is used because 

most errors and warning messages that appear during calibration depend on calibration history.

• Add ISA to both standard solutions and samples.

• Immerse the Ion Selective Electrode and the temperature probe

approximately 2 cm (1”) into the less concentrated standard solution 

and stir gently.

• Select the appropriate standard solution concentration with 

or . For All Standards manual entry mode, the standard

concentration can be selected from a list containing all the predefined and 

custom standards. For Group Standard manual entry mode the standard 

concentration can be selected from the predefined group of standards. 

Press to calibrate the electrode in the standard.

Note: To adjust standard value: Press . A pop-up menu will be displayed on the LCD 

in which the concentration value can be adjusted using or . Press 

to save the new concentration value.

• The “Please wait...” message will appear on the LCD for 10 seconds. Remove ISE from first 

standard, rinse tip and immerse the Ion selective electrode and the temperature probe into the 

next standard solution and follow the above procedure or press to exit calibration.

Notes: The new added calibration point will replace 

an old one if the difference between them is less than 

20 % of the standard solution.

If the existing stored calibration is full (five calibration 

points), a pop-up menu will be displayed on the LCD 

in which you can select with or 

the standard solution you want to replace with the 

current one.

2 cm (1”)

ISE CALIBRATION (HI5222 only)
55

Press to delete the selected calibrated point and then press to update 

calibration with the new standard solution.

If the isopotential point of the electrode is unknown, 

the ISE calibration and measurements can be 

performed without temperature compensation (see 

ISE Setup, Temperature option for details). 

 When in MTC mode, after selecting a standard press 

, a pop-up menu will be displayed on the 

LCD in which the concentration and the temperature 

value can be adjusted by pressing and then 

 or keys. Press to save 

the modified value and then / 

to select next/previous value to be adjusted. MTC 

value will have no effect on measurement but will 

be included on log data.

CALIBRATION MESSAGES

• Wrong standard solution. Check the standard solution: this message appears when the 

difference between the reading and the value of the selected standard solution concentration is 

significant. If this message is displayed, check if you have selected the appropriate calibration 

standard.

• Standard to close. Check the standard or clear calibration: this message appears when 

the difference between current ISE standard and the already calibrated standard is too low. 

• Slope to low/high. Check the standard solution. Recalibrate using fresh standards.

• Difference between standards temperature is too high. Press to update calibration 

or clear old calibration.

ISE CALIBRATION (HI5222 only)
56

Make sure the instrument and ISE sensor have been calibrated before making ISE measurements.

When using one of the incremental methods for measurement, at least a two point ISE calibration 

must be performed to establish the electrode slope.

For accurate measurements, add the appropriate ISA (Ionic Strength Adjuster) to both samples and 

standards. Consult ISE manual for sensor preparation details.

DIRECT MEASUREMENT

To measure the concentration of a sample using the Direct 

reading mode:

• Press and then to select ISE Measure 

mode for the selected channel.

• Select the Direct reading mode (see ISE Setup for 

details).

• Add ISA to the sample solution.

• Submerge the Ion Selective Electrode tip and the 

temperature probe approximately 2 cm (1”) into the 

sample. Allow time for the electrode to stabilize.

• The measured concentration value will be displayed on 

the LCD in the selected units.

Note: If the reading is out of range, “----” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure the concentration of a sample using the 

Direct/AutoHold reading mode:

• Press and then to select ISE Measure 

mode for the selected channel.

• Select the Direct/AutoHold reading mode (see ISE Setup 

for details).

• Add ISA to the sample solution.

• Dip the Ion Selective Electrode tip and the temperature 

probe approximately 2 cm (1”) into the sample to be 

tested.

• The measured concentration value will be displayed on the LCD. Press , the “AutoHold” 

indicator will blink on the LCD until the stability criterion is reached. The concentration value will 

be frozen on the LCD, along with “AutoHold” indicator.

ISE MEASUREMENT (HI5222 only)
57

• To return to normal Measure mode press .

Note: If the reading is out of range, “----” will be displayed on the LCD.

KNOWN ADDITION

To measure the concentration of a sample using the 

Known Addition incremental method:

• Press and then to select ISE 

Measure mode for the selected channel.

• Select the Known Addition method (see ISE Setup for 

details).

• Prior to starting a KA procedure, the ISE sensor must 

be calibrated with a minimum of two standards 

containing ISA. The slope of the electrode will be 

used in all calculations involved in KA.

• If following an established procedure: Press ,

Edit the method variables and follow the procedure 

below.

• Press to set the method parameters. Press 

 / to select next/previous parameter 

to edit, then press and use or 

 to set the desired parameter value. Press 

 to save the modified value and then press 

 to exit method parameters edit menu.

• If developing a procedure: Before attempting Known 

Addition analysis it is important to determine what 

sample volume, standard concentration and standard 

volume will produce the best results. As a general rule, 

the addition of standard should change the mV value 

of the sample by 15 - 20 mV. For a positively charged ion (i.e. Sodium, Potassium, Calcium), the 

standard addition should increase the mV. For a negatively charged ion (i.e. Sulfide, Fluoride, 

Chloride), the standard addition should decrease the mV. Start with a small trial. For example: 

Measure 50 mL of sample, add a magnetic stir bar, place on a stirrer, add ISA (consult ISE 

manual) and place ISE electrode tip into the sample. Put instrument in mV mode and record 

the observed mV. Using a micropipette, add a volume of the highest ISE standard available 

(i.e. 0.1M or 1000 ppm). 

ISE MEASUREMENT (HI5222 only)
58

 Start by adding 500 μL at a time (for example). Watch the change in mV. When you have 

observed approximately a 15 mV change from the original sample, calculate the total volume 

added. Adjust sample and standard volumes proportionally to standard volumes that can be 

measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.

• Press : Edit the procedure variables to the volumes determined in the prior step.

Procedure:

• Press to enter Known Addition mode.

• Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate. 

Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and 

a mV value will show on the display.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of 

the method will be displayed on the LCD in which the user is notified to add the Volume of 

Standard to the sample.

• Press to take the second mV reading.

• When the reading is stable, press to store 

the second mV reading. The ISE measurement results 

will be displayed on the LCD.

• Press to log the current results into a ISE 

Method Report. Press to return to ISE

Measure mode. Press to measure additional 

samples. Rinse ISE sample between samples.

• Press to modify the method parameters.

Note: Press at any time to stop the

measurement and return to ISE Measure mode.

KNOWN SUBTRACTION

To measure the concentration of a sample using the Known Subtraction method:

• Press and then to select ISE Measure mode for the selected channel.

• Select the Known Subtraction method (see ISE Setup → Reading Mode).

• Prior to starting a KS procedure, the ISE sensor must be calibrated with a minimum of two 

standards containing ISA. The slope of the electrode will be used in all calculations involved in 

KS.

ISE MEASUREMENT (HI5222 only)
59

• If following an established procedure: Press then edit the method variables and follow 

the procedure below.

• Press to set the method parameters. Press / to select next/previous parameter to edit, then press and use or to set the desired 

parameter value. Press to save the modified value and then press to exit 

method parameters edit menu.

• If developing a procedure: Before attempting Known Subtraction analysis it is important to 

determine what sample volume, standard reactant concentration and standard volume will 

produce the best results and the way the reagent will react with the measured ion on a molar 

basis (stoichiometric factor). As a general rule, the addition of standard should change the mV 

value of the sample by 15-20 mV.

 For a positively charged ion (i.e. Calcium), the reactant addition should decrease the mV. For 

a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the reactant addition should increase 

the mV. Start with a small trial. For example: Measure 50 mL of sample, add a magnetic stir 

bar, place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip in to the 

sample. Put instrument in mV mode and record the observed mV. Using a micropipette, add 

a volume of the reactant standard. Start by adding 500 μL at a time (for example). Watch 

the change in mV. When you have observed approximately a 15 mV change from the original 

sample, calculate the total volume added. Adjust sample and standard volumes proportionally 

to standard volumes that can be measured with accuracy. Use volumetric pipettes for standard, 

ISA and reagent addition.

• Press then edit the procedure variables to the volumes determined in the prior step.

Procedure:

• Press to enter Known Subtraction mode.

• Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate. 

Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and 

a mV value will show on the display.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the 

method will be displayed on the LCD in which the user is notified to add the volume of reagent 

to the sample.

• Press to take the second mV reading.

ISE MEASUREMENT (HI5222 only)
60

• When the reading is stable, press to store the second mV reading. The ISE measurement 

results will be displayed on the LCD.

• Press to log the current results into an ISE Method Report. Press to return to 

ISE Measure mode. Press to start another measurement. Rinse ISE sensor between 

samples.

• Press , to modify parameters.

Note: Press at any time to stop the measurement and return to ISE Measure mode.

ANALYTE ADDITION

To measure the concentration of a sample using Analyte Addition method:

• Press and then to select ISE Measure mode for the selected channel.

• Select the Analyte Addition method (see ISE Setup → Reading Mode).

• Prior to starting an AA procedure, the ISE sensor must be calibrated with a minimum of two 

standards containing ISA. The slope of the electrode will be used in all calculations involved in 

AA.

• If following an established procedure: Press then edit the method variables and follow 

the procedure below.

• Press to set the method parameters. Press / to select next/previous parameter to edit, then press and use or to set the desired 

parameter value. Press to save the modified value and then press to exit 

method parameters edit menu.

• If developing a procedure: Before attempting Analyte Addition analysis, it is important to 

determine which standard volume, concentration and sample size will produce the best results. 

As a general rule, the standard must be less concentrated than the sample so the addition of 

sample will increase the total ion content of the solution from the beaker and change the mV 

value by at least 10 mV. For a positively charged ion (i.e. Sodium), the AA increases the mV. 

For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the AA should decrease the mV. 

Start with a small trial. For example: Measure 50 mL of standard, add a magnetic stir bar and 

place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put 

instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the 

sample. Start by adding 500 μL at a time (for example). Watch the change in mV. When you 

have observed approximately a 10 mV change from the original standard, calculate the total 

volume added. Adjust sample and standard volumes proportionally to standard volumes that 

can be measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.

ISE MEASUREMENT (HI5222 only)
61

• Press then edit the procedure variables to the volumes determined in the prior step.

Procedure:

• Press to enter Analyte Addition mode.

• Volumetrically add standard to a clean beaker. Add a magnetic stir bar and place on a stir plate. 

Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution 

and a mV value will show on the display.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the 

method will be displayed on the LCD, in which the user is notified to add the Sample Volume 

to the standard solution. The method parameters are also displayed on the LCD.

• Press to take the second mV reading.

• When the reading is stable, press to store the second mV reading. The ISE measurement 

results will be displayed on the LCD.

• Press to log the current results into an ISE Method Report. Press to return to 

ISE Measure mode.

• Press to start another measurement. Rinse ISE sensor between samples.

• Press , to modify the method parameters.

 Note: Press at any time to stop the measurement and return to ISE Measure mode.

ANALYTE SUBTRACTION

To measure the concentration of a sample using Analyte Subtraction method:

• Press and then to select ISE Measure mode for the selected channel.

• Select the Analyte Subtraction method (see ISE Setup → Reading Mode).

• Prior to starting an AS procedure, the ISE sensor must be calibrated with a minimum of two 

standards containing ISA. The slope of the electrode will be used in all calculations involved in 

AS.

• If following an established procedure: Press then edit the method variables and follow 

the procedure below.

• Press to set the method parameters. Press / to select next/previous parameter to edit, then press and use or to set the desired 

parameter value. Press to save the modified value and then press to exit 

method parameters edit menu.

ISE MEASUREMENT (HI5222 only)
62

• If developing a procedure: Before attempting Analyte Subtraction analysis, it is important to 

determine which sample volume, reactant volume and concentration, will produce the best results 

and the way the reagent will react with the measured ion on a molar basis (stoichiometric factor). As a general rule, the reactant should contain the measured ion so the sample addition 

will react with the ion and reduce the measured concentration of the sample. The change of the 

mV value, before and after the sample addition, should be at least 10 mV. Start with a small 

trial. For example: Measure 50 mL of reactant, add a magnetic stir bar and place on a stirrer, 

add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put instrument in 

mV mode and record the observed mV. Using a micropipette, add a volume of the sample. 

Start by adding 500 μL at a time (for example). Watch the change in mV. When you have 

observed approximately a 10 mV change from the original value, calculate the total volume 

added. Adjust sample and standard volumes proportionally to standard volumes that can be 

measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition. 

• Press then edit the procedure variables to the volumes determined in the prior step.

Procedure:

• Press to enter Analyte Subtraction mode.

• Volumetrically add reactant to a clean beaker. Add a magnetic stir bar and place on a stir plate. 

Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution 

and a mV value will show on the display.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the 

method will be displayed on the LCD in which the user is notified to add the Sample Volume 

to the standard solution.

• Press to take the second mV reading.

• When the reading is stable, press to store the second mV reading. The ISE measurement 

results will be displayed on the LCD.

• Press to log the current results into an ISE Method Report. Press to return 

to ISE Measure mode. Press to start another measurement. Rinse sensor between 

samples.

• Press to modify the method parameters.

Note: Press at any time to stop the measurement and return to ISE Measure mode.

ISE MEASUREMENT (HI5222 only)
63

The user temperature calibration menu can be accessed during meter startup by simultaneously 

pressing three keys as shown in the drawing below. Press the keys after the short beep is heard at 

the meter power on. Keep all three keys pressed until Temp. Calibration menu appears.

Note: The user temperature calibration is performed at three points: around 0 °C, 50 °C and

100 °C.

To perform the user temperature calibration:

• Select the desired temperature channel by pressing 

 (HI5222 only).

• Press to start the temperature calibration. 

Adjust the temperature preset value using 

or when necessary.

• Insert the temperature probe into the beaker with water 

at 0 °C.

• Wait for measurement to stabilize and then press 

 to confirm the calibration point.

• Repeat the previous steps for 50 °C and 100 °C.

• Save the calibration.

• Press to return to measure mode.

Note: Press if you want to clear the temperature user calibration.

TEMPERATURE CALIBRATION
64

Reading Mode Log log Recall

Automatic (1) Automatic Log

Direct Manual (2) Manual Log

Auto Hold (NA) Not Applicable

Automatic (3) Automatic Log

Direct/AutoHold Manual (4) Manual Log

Auto Hold (5) Manual Log

There are 5 ways the Reading Mode and Log may be configured together. The table below shows 

the combinations and indicates where the completed log will be stored.

1) Direct Reading Mode and Automatic Log:

Real time continuous measurements are on display with 

continuous logging to meter memory. Press .

LOGGING
65

2) Direct Reading Mode and Manual Log:

Real time continuous measurements are on display and 

snapshots of measurement data are stored in the Manual 

log when the user presses . Subsequent snapshots 

will be added to the same Manual Lot every time the 

 is depressed unless New Lot is selected under 

Log options.

Note: When the is pressed the lot ID along 

with the current record number will appear for 

short time on the selected channel window on the

top/left corner (e.g. L033_MV 8 - this means lot 

ID L033_mV and recod number 8).

3) Direct/AutoHold Reading Mode and Automatic Log

Press and then keys must be pressed 

on front display to initiate this function. Real time continuous 

measurements are on display with “AutoHold” flashing 

and real time continuous logging into meter memory, 

until the meter reaches the stability criteria to go into 

AutoHold mode. The stored sample logs will be marked 

with an “H” to indicate the AutoHold mode. The virtual 

key returns operation to real time continuous 

measurements and stops the logging session.

4) Direct/AutoHold Reading Mode and Manual Log

Press in order to add one new record in the log report. The manual log is working even 

if it is in AutoHold or Continuous Reading mode. Press to initiate the AutoHold event. 

“AutoHold” will flash until the stability criteria is reached and then the screen freezes in Auto 

Hold mode, the data is marked with an “H”.

LOGGING
66

5) Direct/AutoHold Reading Mode and Auto Hold Log

Press and then keys initiate and automate the capture of stable data which is 

stored in the Recall Manual Log file. During the process, “AutoHold” will flash until the stability 

criteria is reached and then the screen freezes in AutoHold mode, the data is logged and marked 

with an “H”. The virtual key returns operation to Real time continuous measurement. 

Press again to log a second stable data point. The lot ID along with the record index will 

appear for short time on the top/left corner on the selected channel window, every time a record 

will be added to the lot.

LOG RECALL

This feature allows the user to view all stored data. If 

no data was logged, the “No records were found” 

message will be displayed on the LCD in the Log Recall 

screen. Otherwise, the instrument will display all the 

memorized lots in accordance with the selected option: 

Automatic Log, Manual Log or ISE Method Report

(HI5222 only).

To view the memorized data:

• Press while in Measure mode.

• Press . The “Choose Log Report Type” 

message will be displayed in the Reminder

messages area.

LOGGING

• Press , or to select the 

desired Log Report type. All logged lots for the selected 

Log Report type will be displayed on the LCD.

• To filter the displayed lots, press and then 

the desired parameter , or 

-HI5222 only. Only the selected measurement

parameter lots will be displayed on the LCD.
67

• Select the desired lot with or and 

press to display the logged data from the 

highlighted lot. The “Please wait...” message will 

be displayed on the LCD for one second. The selected 

Logging Data Configuration options will be displayed 

on the LCD, together with GLP information (last 

calibration date and calibrated buffers/standards) 

if a calibration has been performed on the selected 

mode and the logged values (measured value, mV 

value, temperature value, temperature compensation 

mode and the logging time).

Note: For automatic logging only, it is possible to view the plotted graph.

Press to display the graph.

By pressing it is possible to move the graph 

along the X or Y axis with the arrow keys.If pressing 

 while the graph is displayed, the 

zoom menu for the X and Y axes will be accessed. 

Press or / /

 / to switch between the

active zooming axes and then zoom in or out 

on the selected axis by pressing the appropriate 

virtual key. 

• Press to return to the previous menu at 

any time.

LOGGING
68

Data transmission from the instrument to the PC can be done with the HI92000 Windows®

compatible software (optional). HI92000 also offers graphing and on-line help features.

Data logged on the HI5221 and HI5222 meters can be exported to the most popular spreadsheet 

programs for further analysis.

HI5221 and HI5222 instruments have an USB interface available.

Use a standard USB cable to connect your instrument to the PC.

Make sure that the instrument and the HI92000 software have the same baud rate and the

appropriate communication port.

The PC software may also be used for real time logging.

To delete lots:

• Press while in Log Recall mode.

• Press or to access delete or delete 

all mode. Otherwise, press to return to Log 

Recall view mode.

• After selecting one of the deleting modes, use 

or to select one lot and then press 

or to delete the selected lot or all lots. The 

“Please wait...” message will be displayed on the 

LCD until the selected lot or all lots are deleted.

• Press and then press to exit deleting 

mode and return to Log Recall view mode.

• Press to exit Log Recall mode and return 

to Measure mode.

Note: Logged lots should also be deleted whenever “Please Delete Old Log Files” or “Low Data 

Logging Space” message appears on the LCD, in the Reminder messages area.

LOGGING PC INTERFACE
69

ISE THEORY

An Ion Selective Electrode (ISE) is an electrochemical sensor that changes voltage with the activity or 

concentration of ions in solutions. The change in voltage is a logarithmic relationship with concentration, 

and is expressed by the Nernst equation:

where: E - the measured voltage;

Eo - standard voltage and other standard system voltages;

a - the activity of the ion being measured;

S - the Nernst slope factor and is derived from thermodynamic principles:

R - the universal gas constant (8.314 J/(K⋅mol));

T - the temperature in degrees Kelvin;

F - the Faraday’s constant (96,485 C/mol);

n - the ion charge.

The slope may be positive or negative depending upon the Ion charge (n).

SPECIES SLOPE

(mV/decade)

Monovalent cation +59.16

Monovalent anion –59.16

Divalent cation +29.58

Divalent anion –29.58

E = Eo + S log(a)

S = 

2.303RT

nF

Activity and concentration are related by an “activity coefficient”, expressed as:

where: a - the activity of the ion being measured;

γ - the activity coefficient;

C - the concentration of the ion being measured.

a = γ · C

ADDITIONAL INFORMATION
70

ION SELECTIVE ANALYSIS METHODS 

Direct Analysis

This method is a simple procedure for measuring multiple samples. It should only be used in the 

linear working regions of the sensor. A direct reading instrument such as the HI5222 determines 

concentration of the unknown by a direct reading after calibrating the instrument with the standards. 

The instrument is calibrated as described in “ISE CALIBRATION & MEASUREMENTS” section, with two 

or more freshly made standards that are in the measurement range of the unknowns. Ionic strength 

adjustment is made to samples and standards. Unknowns are measured directly by the instrument.

At lower concentrations, in non-linear regions of the electrode response, multiple calibration points will 

extend measurements to a practical detection limit. Calibrations must be performed more frequently 

in these cases.

Incremental Methods

Incremental methods are useful for the measurement of samples whose constituents are variable 

or concentrated. Incremental techniques can reduce errors from such variables as temperature, 

viscosity, or pH extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement. There are four commonly used different incremental methods for 

sample measurement. They are Known Addition, Known Subtraction, Analyte Addition and Analyte 

Subtraction. HI5222 allows the analyst to use these techniques as a simple routine procedure, thus 

eliminating calculations or tables. The method once set up can be used for repetitive measurements 

on multiple samples.

Known Addition and Known Subtraction

With Known addition, standard is added to a sample being measured. The standard and sample 

contain the same ion. mV are taken before and after the standard addition. From the change in 

mV, the sample concentration is determined.

E = Eo + S log(C)

CSAMP = CSTD · VSTD · 

(VSAMP + VISA)

(VSAMP + VSTD + VISA)·10∆E- (VSAMP + VISA) VSAMP

S

In very dilute solutions γ approaches 1 so activity and concentration are the same.

Actual samples that are more concentrated have much smaller activity coefficients (γ < 1). The 

addition of an inert background salt to standards and samples stabilizes the activity coefficient so that 

concentration measurements may be made directly. Some of Hanna’s Ionic Strength Adjuster formulations also may optimize pH and complex interferences, in addition to standardizing the ionic strength.

The Nernst equation can be written:

ADDITIONAL INFORMATION
71

CSAMP = CSTD · VSTD · f · 

(VSAMP + VISA)

(VSAMP + VISA) - (VSAMP + VSTD + VISA)·10∆E VSAMP

S

∆E - the difference of potential from the electrode;

S - the electrode slope, determined in a previous calibration;

f - the stoichiometric ratio between sample 

and standard;

where: CSAMP - the sample concentration;

CSTD - the standard concentration;

VSAMP - the sample volume;

VSTD - the standard volume;

VISA - ISA volume

Example 1

You have sulfide samples and you are adding Ag+. The reaction is:

One mole sulfide sample reacts with 2 moles silver standard (f = 1/2).

Example 2

You have sulfide samples and you are adding Pb2+. The reaction is:

One mole sulfide sample reacts with 1 mole lead standard (f = 1).

Analyte Addition and Analyte Subtraction

Analyte Addition and Subtraction are variations of the previous two methods.

With Analyte Addition, sample (analyte) is added to an ion standard being measured. The 

standard and sample contain the same ion. mV are taken before and after the sample addition. 

From the mV the analyte concentration is determined.

With Analyte Subtraction, sample (analyte) is added to an ion standard being measured. The 

analyte reacts with the measured ion in a known manner thus removing measured ions from the 

solution. From the change in mV the concentration of the analyte is determined.

S2- + 2Ag+ → Ag2S

S2- + Pb2+ → PbS

CSAMP =

 CSTD · VSTD · (VSTD + VSAMP + VISA)·10∆E- (VSTD + VISA)

(VSTD + VISA) VSAMP

S

With Known subtraction, a known standard is added to an ionic sample being measured. The 

standard reacts with the measured ion in the sample in a known manner, thus removing measured 

ions from the solution. From the change in mV, the concentration of the sample is determined.

CSAMP = f ·{(VSTD + VISA) - [1 + (VSTD + VISA)]·10∆E}·(CSTD·VSTD ) VSAMP VSAMP VSTD + VISA

S

ADDITIONAL INFORMATION
72

Temperature has an effect on pH. The calibration buffer solutions are affected by temperature 

changes to a lower degree than normal solutions.

During calibration the instrument will automatically calibrate to the pH value corresponding to the 

measured or set temperature.

TEMP pH BUFFERS

°C K °F 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.454

0 273 32 1.670 3.072 4.007 6.982 7.130 9.459 10.316 13.379

5 278 41 1.670 3.051 4.002 6.949 7.098 9.391 10.245 13.178

10 283 50 1.671 3.033 4.000 6.921 7.070 9.328 10.180 12.985

15 288 59 1.673 3.019 4.001 6.897 7.046 9.273 10.118 12.799

20 293 68 1.675 3.008 4.004 6.878 7.027 9.222 10.062 12.621

25 298 77 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.450

30 303 86 1.683 2.995 4.017 6.851 6.998 9.137 9.962 12.286

35 308 95 1.688 2.991 4.026 6.842 6.989 9.108 9.919 12.128

40 313 104 1.693 2.990 4.037 6.837 6.983 9.069 9.881 11.978

45 318 113 1.700 2.990 4.049 6.834 6.979 9.040 9.847 11.834

50 323 122 1.707 2.991 4.062 6.834 6.978 9.014 9.817 11.697

55 328 131 1.715 2.993 4.076 6.836 6.979 8.990 9.793 11.566

60 333 140 1.724 2.995 4.091 6.839 6.982 8.969 9.773 11.442

where: CSAMP - the sample concentration;

CSTD - the standard concentration;

VSAMP - the sample volume;

VSTD - the standard volume;

VISA - ISA volume;

∆E- the difference of potential from the electrode;

S - the electrode slope, determined in a previous 

calibration;

f - the stoichiometric ratio between sample and 

standard;

65 338 149 1.734 2.998 4.107 6.844 6.987 8.948 9.757 11.323

70 343 158 1.744 3.000 4.123 6.850 6.993 8.929 9.746 11.211

75 348 167 1.755 3.002 4.139 6.857 7.001 8.910 9.740 11.104

80 353 176 1.767 3.003 4.156 6.865 7.010 8.891 9.738 11.003

85 358 185 1.780 3.002 4.172 6.873 7.019 8.871 9.740 10.908

90 363 194 1.793 3.000 4.187 6.880 7.029 8.851 9.748 10.819

95 368 203 1.807 2.996 4.202 6.888 7.040 8.829 9.759 10.734

During calibration the instrument will display the pH buffer value at 25 °C.

pH BUFFER TEMPERATURE DEPENDENCE
73

Reference

Fill Hole

Sensing

Wire

Reference

Wire

Reference

Junction

Glass

Bulb

Reference

Fill Hole

Reference

Wire

Sensing

Wire

Reference

Junction

Glass

Bulb

Glass Body

pH Electrode

Plastic Body

pH Electrode

Reference

Wire

Reference

Junction

Platinum or

Gold tip

Plastic Body

ORP Electrode

Glass Body

ORP Electrode

Reference

Wire

Reference

Junction

Platinum or

Gold tip

ELECTRODE CONDITIONING AND MAINTENANCE

PREPARATION PROCEDURE

Remove the protective cap off the pH electrode. 

SALT DEPOSITS MAY BE PRESENT. This is normal with electrodes. They will disappear when rinsed 

with water.

During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning 

of the electrode. These bubbles can be removed by “shaking down” the electrode as you would 

do with a glass thermometer.

If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300 Storage Solution 

for at least one hour.

For refillable electrodes:

If the filling solution (electrolyte) is more than 2 cm (1”) below the fill hole, add HI7082 or

HI8082 3.5M KCl Electrolyte Solution for double junction or HI7071 or HI8071 3.5M KCl+AgCl 

Electrolyte Solution for single junction electrodes.

Unscrew the fill hole screw during measurements. This will allow electrolyte to flow out of the junction.
74

For AmpHel® electrodes:

If the electrode does not respond to pH changes, the battery may have run down and the electrode 

or battery (if replaceable) should be replaced.

MEASURE

Rinse the pH electrode tip with distilled water. Immerse the tip bottom 4 cm (1.5”) in the sample 

and stir gently for a few seconds.

For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip with 

a few drops of the solution to be tested, before taking measurements.

STORAGE PROCEDURE

To minimize clogging and ensure a quick response time, the glass bulb and the junction of pH 

electrode should be kept moist and not allowed to dry out.

Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage

Solution or, in its absence, Filling Solution (HI7071 or HI8071 for single junction and HI7082 or

HI8082 for double junction electrodes). Follow the Preparation Procedure before taking

measurements.

 Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

PERIODIC MAINTENANCE

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact 

and there must be no points of broken insulation on the cable or cracks on the electrode stem or 

bulb. Connectors must be perfectly clean and dry. 

If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water.

pH PROBE MAINTENANCE

For refillable electrodes:

Refill the reference chamber with fresh electrolyte (HI7071 or HI8071 for single junction or

HI7082 or HI8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. 

Follow the Storage Procedure above.

pH CLEANING PROCEDURE

• General Soak in Hanna HI7061 or HI8061 General Cleaning Solution for approximately 

30 minutes.

• Protein Soak in Hanna HI7073 Protein Cleaning Solution for 15 minutes.

• Inorganic Soak in Hanna HI7074 Inorganic Cleaning Solution for 15 minutes. This is good 

at cleaning a black ceramic junction.

• Oil/grease Rinse with Hanna HI7077 or HI8077 Oil and Fat Cleaning Solution.

ELECTRODE CONDITIONING AND MAINTENANCE
75

TROUBLESHOOTING GUIDE

SYMPTOMS PROBLEM SOLUTION

Slow response/excesive drift Dirty pH electrode.

Dirty reference junction.

Soak the electrode tip in 

HI7061 solution for 30 min.

and then clean the electrode.

Soak in HI7074.

Readings fluctuate up and 

down (noise).

Clogged/dirty junction.

Low electrolyte level (refilable 

electrodes only).

Clean the electrode.

Refill with fresh solution (for 

refilable electrodes only).

The LCD displays “_” during 

measurements (pH, mV, mV 

Rel or ISE).

Out of range in the appropriate scale.Check to see if the pH bulb 

and reference junction are in 

the solution. Make sure the 

sample is in the specified 

range.Check the electrolyte 

level and the general state of 

the pH/ORP or ISE electrode.

IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly 

with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled 

electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour 

before taking measurements.

Out of range in the mV scale. Dry reference junction. Soak in HI70300 Storage 

solution for at least one hour.

The instrument does not work 

with the temperature probe.

Out order temperature probe. Replace the probe.

The meter fails to calibrate or 

gives faulty readings.

Broken or out of order 

electrode.

Replace the electrode.

Explicit warnings are displayed during calibration.Dirty/broken electrode, 

contaminated buffers.

Follow displayed instructions.

The electrode condition is not 

displayed after calibration.

Only one-point calibration has 

been performed.

Perform at least a two-point 

calibration.

The instrument does not 

start up.

Internal or software error. Restart the instrument using 

the power switch or unplug 

adapter.If the error persists, 

contact your vendor.
76

Since the resistance of the pH electrode is in the range of 50 – 200 MΩ, the current across the 

membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode 

for many hours.

The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, 

the electrode life is drastically reduced.

Typical Electrode Life

Ambient Temperature 1 – 3 years

90 °C (194 °F) Less than 4 months

120 °C (248 °F) Less than 1 month

The resistance of glass electrodes partially depends on the temperature. The lower the temperature, 

the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher.

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS

Alkaline Error

High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which 

the interference starts to be significant depends upon the composition of the glass. This interference 

is called alkaline error and causes the pH to be underestimated.
77

ACCESSORIES

pH BUFFER SOLUTIONS

HI6016 pH 1.679 Buffer Solution, 500 mL bottle

HI6003 pH 3.000 Buffer Solution, 500 mL bottle

HI8004L pH 4.01 Buffer Solution in FDA approved bottle, 500 mL

HI6004 pH 4.010 Buffer Solution, 500 mL bottle

HI8006L pH 6.86 Buffer Solution in FDA approved bottle, 500 mL

HI6068 pH 6.862 Buffer Solution, 500 mL bottle

HI8007L pH 7.01 Buffer Solution in FDA approved bottle, 500 mL

HI6007 pH 7.010 Buffer Solution, 500 mL bottle

HI8009L pH 9.18 Buffer Solution in FDA approved bottle, 500 mL

HI8010L pH 10.01 Buffer Solution in FDA approved bottle, 500 mL

HI6010 pH 10.010 Buffer Solution, 500 mL bottle

HI6124 pH 12.450 Buffer Solution, 500 mL bottle

ELECTRODE STORAGE SOLUTIONS

HI70300L Storage Solution, 500 mL bottle

HI80300L Storage Solution in FDA approved bottle, 500 mL

ELECTRODE CLEANING SOLUTIONS

HI70000P Electrode Rinse Sachets, 20 mL, 25 pcs

HI7061L General Purpose Solution, 500 mL bottle

HI7073L Protein Cleaning Solution, 500 mL bottle

HI7074L Protein Cleaning Solution, 500 mL bottle

HI7077L Oil & Fat Cleaning Solution, 500 mL bottle

HI8061L General Purpose Solution in FDA approved bottle, 500 mL

HI8073L Protein Cleaning Solution in FDA approved bottle, 500 mL

HI8077L Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL

ELECTRODE REFILL ELECTROLYTE SOLUTIONS

HI7071 3.5M KCl + AgCl Electrolyte, 4x30 mL, for single junction electrodes

HI7072 1M KNO3 Electrolyte, 4x30 mL

HI7082 3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
78

pH ELECTRODES

All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3’) cable, 

as shown below:

HI1043B

Glass body, double junction, refillable, combination pH electrode.

Use: strong acid/alkali.

HI1053B

Glass body, triple ceramic, conical shape, refillable, combination pH electrode.

Use: emulsions.

ACCESSORIES

HI8071 3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL, for single 

junction electrodes

HI8072 1M KNO3 Electrolyte in FDA approved bottle, 4x30 mL

HI8082 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes

HI8093 1M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL

ORP SOLUTIONS

HI7020L Test Solution 200-275 mV, 500 mL bottle

HI7021L Test Solution 240 mV, 500 mL bottle

HI7022L Test Solution 470 mV, 500 mL bottle

HI7091L Reducing Pretreatment Solution

HI7092L Oxidizing Pretreatment Solution, 500 mL
79

HI1083B

Glass body, micro, Viscolene, non refillable, combination pH electrode.

Use: biotechnology, micro titration.

HI1131B

Glass body, refillable, double junction, combination pH electrode. 

Use: general purpose.

HI1330B

Glass body, semimicro, single junction, refillable, combination pH electrode.

Use: laboratory, vials.

HI1331B

Glass body, semimicro, single junction, refillable, combination pH electrode.

Use: flasks.

HI1230B

Plastic body (PEI), double junction, gel filled, combination pH electrode.

Use: general, field.

ACCESSORIES
80

Plastic body (PEI), double junction, refillable, combination pH electrode.

Use: general purpose.

HI1413B

Glass body, single junction, flat tip, Viscolene, non refillable, combination pH electrode.

Use: surface measurement.

FC100B

Plastic body (PVDF), double junction, refillable, combination pH electrode.

Use: general purpose for food industry.

HI2031B

Glass body, semimicro, conical, single junction, refillable, combination pH electrode.

Use: semisolid products.

HI1332B

FC200B

Plastic body (PVDF), single junction, conical, Viscolene, non refillable, combination pH electrode.

Use: meat & cheese.

ACCESSORIES
81

FC210B

Glass body, double junction, conical, Viscolene, non refillable, combination pH electrode.

Use: milk, yogurt.

FC220B

Glass body, triple ceramic, single junction, refillable, combination pH electrode.

Use: food processing.

FC911B 

Plastic body (PVDF), double junction, refillable with built-in amplifier, combination pH electrode.

Use: very high humidity.

ORP ELECTRODES

HI3131B

Glass body, refillable, combination platinum ORP electrode. 

Use: titration.

HI3230B

Plastic body (PEI), gel filled, combination platinum ORP electrode.

Use: general purpose.

ACCESSORIES
82

CONNECT TO

SCREW TYPE

ELECTRODES

CONNECT TO THE

BNC SOCKET

OF THE METER

HI 7855 SERIES CABLE CONNECTORS

CONNECTOR AND 3.0 mm (0.12") CABLE WITH BNC

HI4430B

Plastic body (PEI), gel filled, combination gold ORP electrode. 

Use: general purpose.

Consult the Hanna General Catalog for more electrodes with screw-type or BNC connectors.

EXTENSION CABLE FOR SCREW-TYPE ELECTRODES

(SCREW TO BNC ADAPTER)

HI7855/1 Extension cable 1 m (3.3’) long

HI7855/3 Extension cable 3 m (9.9’) long

ACCESSORIES

OTHER ACCESSORIES

HI710005/8 Voltage adapter from 120 Vac / 12 Vdc 800 mA (USA plug)

HI710006/8 Voltage adapter from 230 Vac / 12 Vdc 800 mA (European plug)

HI76404W Electrode holder

HI8427 pH and ORP electrode simulator with 1 m (3.3’) coaxial cable ending in 

female BNC connectors

HI931001 pH and ORP electrode simulator with LCD and 1 m (3.3’) coaxial cable ending 

in female BNC connectors

HI7662-W Temperature probe with 1 m (3.3’) cable

HI92000 Windows® compatible software

HI920013 USB cable
Hanna Instruments reserves the right to modify the design, construction or appearance of it’s 

products without advance notice.

Before using this product, make sure that it is entirely 

suitable for your specific application and for the 

environment in which it is used. 

Operation of this instrument may cause interference to 

other electronic equipment, requiring the operator to take 

steps to correct interference. Any variation introduced 

by the user to the supplied equipment may degrade the 

instrument’s EMC performance.

To avoid damages or burns, do not put the instrument in 

microwave ovens. For your and the instrument’s safety, do 

not use or store the instrument in hazardous environments.

Recommendations for 

Users

HI5221 and HI5222 are warranted for two years against defects in 

workmanship and materials when used for its intended purpose and 

maintained according to instructions. Electrodes and probes are warranted 

for six months. This warranty is limited to repair or replacement free of 

charge. Damage due to accidents, misuse, tampering or lack of prescribed 

maintenance is not covered. If service is required, contact your local Hanna 

Office. If under warranty, report the model number, date of purchase, serial 

number and the nature of the problem. If the repair is not covered by the 

warranty, you will be noticed of the charges incurred. If the instrument 

is to be returned to Hanna Instruments, first obtain a Returned Goods 

Authorization (RGA) number from the Technical Service department and then 

send it with shipping costs prepaid. When shipping any instrument, make 

sure it is properly packed for complete protection.

Warranty
MAN5222 05/16 Printed in ROMANIA

Local Sales and Customer Service Office

World

Headquarters

Hanna Instruments Inc.

Highland Industrial Park

584 Park East Drive

Woonsocket, RI 02895 USA

www.hannainst.com

Local Office

Hanna Instruments USA

270 George Washington Highway

Smithfield, RI 02917

Phone: 800.426.6287

Fax: 401.765.7575

e-mail: tech@hannainst.com

Printed in ROMANIA