Crime Scene Investigation

2013

A Guide For Law Enforcement

Bureau of Justice Assistance

U.S. Department of Justice
CRIME SCENE INVESTIGATION A Guide for Law Enforcement 

www.nfstc.org i

Crime Scene Investigation A Guide for Law Enforcement 

September 2013

Original guide developed and approved by the Technical Working 

Group on Crime Scene Investigation, January 2000. 

Updated guide developed and approved by the Review Committee, 

September 2012. 

Project Director: Kevin Lothridge 

Project Manager: Frank Fitzpatrick

National Forensic Science Technology Center

7881 114th Avenue North 

Largo, FL 33773
CRIME SCENE INVESTIGATION 

A Guide for Law Enforcement 

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Revision of this guide was conducted by the National Forensic 

Science Technology Center (NFSTC), supported under cooperative 

agreements 2009-D1-BX-K028 and 2010-DD-BX-K009 by the 

Bureau of Justice Assistance, and 2007-MU-BX-K008 by the National 

Institute of Justice, Office of Justice Programs, U.S. Department of Justice. 

This document is not intended to create, does not create, and may 

not be relied upon to create any rights, substantive or procedural, 

enforceable at law by any party in any matter, civil or criminal. 

Opinions or points of view expressed in this document represent a 

consensus of the authors and do not necessarily reflect the official 

position of the U.S. Department of Justice. 
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Contents 

A. Arriving at the Scene: Initial Response/ Prioritization of Efforts ................................. 1

1. Initial Response/ Receipt of Information .......................................................................... 1

2. Safety Procedures ................................................................................................................... 2

3. Emergency Care...................................................................................................................... 2

4. Secure and Control Persons at the Scene ............................................................................ 3

5. Boundaries: Identify, Establish, Protect and Secure .......................................................... 4

6. Turn Over Control of the Scene and Brief Investigator(s) in Charge ............................ 6

7. Document Actions and Observations ................................................................................. 6

8. Establish a Command Post (Incident Command System) and Make Notifications..... 7

9. Manage Witnesses................................................................................................................... 8

B. Preliminary Documentation and Evaluation of the Scene .......................................... 10

1. Conduct Scene Assessment................................................................................................. 10

2. Conduct Scene “Walk-Through” and Initial Documentation .......................................11

3. Note-Taking and Logs .........................................................................................................13

C. Processing the Scene .................................................................................................... 15

1. Determine Team Composition...........................................................................................15

2. Ensure Contamination Control ..........................................................................................15

3. Documentation .....................................................................................................................16

Sketching ........................................................................................................................... 17

Photography...................................................................................................................... 27

Videography ...................................................................................................................... 35

4. Prioritize Collection of Evidence .......................................................................................38

5. Crime Scene Search Methods.............................................................................................39

6. Collect, Preserve, Inventory, Package, Transport, and Submit Evidence....................42

7. Detailed Crime Scene Evidence Collection......................................................................42

Ignitable Liquids ............................................................................................................... 43

Bodily Fluids ..................................................................................................................... 43

Male Suspect Evidence Collection, Including Sexual Assault .......................................... 54

Bombs and Explosives ...................................................................................................... 56
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Documents ........................................................................................................................ 57

Firearms............................................................................................................................ 61

Ammunition ....................................................................................................................... 67

Tool Mark Evidence .......................................................................................................... 71

Trace Evidence ................................................................................................................. 73

Footwear and Tire Impressions ........................................................................................ 98

Footwear Impression Evidence ....................................................................................... 101

Motor Vehicles ................................................................................................................ 111

Electronic and Digital Evidence ..................................................................................... 113

Fingerprints .................................................................................................................... 134

Comparison/Elimination Prints ...................................................................................... 139

Tool Mark Evidence ........................................................................................................ 142

D. Completing and Recording the Crime Scene Investigation ...................................... 145

1. Establish Crime Scene Debriefing Team....................................................................... 145

2. Perform Final Survey of the Crime Scene ..................................................................... 146

3. Documentation of the Crime Scene ............................................................................... 146

4. Acknowledge Specialized Crime Scene Circumstances ............................................... 147

Crime Scene Investigation in Correctional and Custodial Facilities ............................. 148

Time-Limited Crime Scene Investigation ........................................................................ 149

E. Crime Scene Equipment ............................................................................................ 150

1. Initial Responding Officer(s) ........................................................................................... 150

2. Crime Scene Investigator/Evidence Technician .......................................................... 151

3. Evidence Collection Kits (Examples) ............................................................................. 153

F. Appendices ..................................................................................................................... i

Appendix A. Glossary..........................................................................................................................ii

Appendix B. Reference List.............................................................................................................viii

Appendix C. Diagrams ........................................................................................................................ x
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A Guide for Law Enforcement 

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Acknowledgements 

This updated Crime Scene Investigation: A Guide to Law 

Enforcement is a revision of the original publication published in 

January 2000, and borrows heavily from that work. The original 

publication was based upon the work of the National Crime Scene 

Planning Panel and additional Technical Working Group Members. 

Their contributions remain as vital today as when the original Guide 

was published. 

To develop this expanded edition, a Review Committee of 

recognized experts was assembled. This committee selected 

additional material from content developed for Department of 

Justice−funded crime scene projects, Scientific Working Groups and 

other open-source documents, which are reflected in the Reference 

section. Additional vetting of the material was accomplished through 

recognized subject matter experts. 

NFSTC wishes to thank the Bureau of Justice Assistance (BJA), the 

National Institute of Standards and Technology (NIST) and the 

National Institute of Justice (NIJ) for providing input to this project. 

The resulting document includes detailed procedural guides for the 

complete range of crime scene investigation tasks – from securing the 

scene to submitting the evidence. This publication provides law 

enforcement professionals and first responders step-by-step guidance 

in this crucial first phase of the justice process. 

Agencies are encouraged to use this reference to enhance existing 

training and promote quality. While these methods can be 

implemented at any agency, jurisdictions will want to carefully 

consider the procedures and their applicability to local agencies and 

circumstances. 
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National Crime Scene Planning Panel 

(NCSPP) 

January 2000

Dr. José R. Almirall, Associate Director/ 

Assistant Professor

International Forensic Research Institute

Department of Chemistry 

Florida International University 

Miami, Florida

Susan Ballou, Forensic Scientist 

Montgomery County Police Department 

Crime Laboratory 

Rockville, Maryland

Paul Carroll, Sergeant (Ret.)

Chicago Police Department

Big Pine Key, Florida

Elizabeth Farris, Chief Trial Counsel 

Hampden County District Attorney’s Office

Springfield, Massachusetts 

Jo Ann Given, ASCLD/LAB

Naval Criminal Investigative Service

Norfolk, Virginia

Marjorie Harris, Senior Forensic Scientist

Department of Criminal Justice 

Division of Forensic Science 

Richmond, Virginia 

Larry McCann, Senior Special Agent (Ret.) 

Virginia State Police 

Richmond, Virginia 

Dr. Joseph L. Peterson 

Department of Criminal Justice 

University of Illinois

Chicago, Illinois

Elliot B. Spector, Director

Center for Police and Security Training 

Suffield, Connecticut 

 

Ann Talbot, ASCLD/LAB

Albuquerque Police Department 

Albuquerque, New Mexico

James T. “Tom” Thurman, Associate 

Professor

College of Law Enforcement 

Eastern Kentucky University 

Richmond, Kentucky 
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Additional Technical Working Group 

Members

Hal R. Arenstein, Attorney at Law

Law Offices of Hal Arenstein

Cincinnati, Ohio

Dexter J. Bartlett, Inspector 

Illinois State Police

Crime Scene Services Command

Joliet, Illinois

Eric Buel, Director

Department of Public Safety

Crime Laboratory

Waterbury, Vermont 

Jeff Cover, Supervisor

Crime Scene Unit

Anne Arundel County Police Department 

Millersville, Maryland

Elizabeth Devine, Supervising Criminalist 

Scientific Services Bureau

Los Angeles County Sheriff’s Department 

Los Angeles, California

Henry Escobar, Detective

San Antonio Police Department 

San Antonio, Texas 

Jerry N. Estes, District Attorney General

10th Judicial District

Athens, Tennessee

 

James Estrada, Detective Investigator 

Homicide Unit 

San Antonio Police Department 

San Antonio, Texas 

Drew Findling, Attorney

Atlanta, Georgia

Nan Horvat/John Sarcone

Assistant Polk County Attorney/ Polk County 

Attorney

Des Moines, Iowa 

N. Michael Hurley, Regional Director 

Oregon State Police

Forensic Services Division

Springfield, Oregon

Gary L. Kaldun, Forensic Scientist, Crime

Scene Coordinator

Bureau of Criminal Apprehension 

St. Paul, Minnesota 

Joe Marchan, Supervising Criminalist 

Texas Department of Public

Safety Crime Laboratory

McAllen, Texas

Joseph John Moseley, II, Detective

Central Homicide Evaluation Support Squad 

Chicago Police Department

Chicago, Illinois

 

Robert Mullins, Detective Investigative 

Services

New Haven Police Department

New Haven, Connecticut

Steve Nash, Detective

Marin County Sheriff’s Department

San Rafael, California

Kathryn Normington-Hollenbach, Senior 

Forensic Scientist

Wyoming State Crime Laboratory

Cheyenne, Wyoming
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Galen Paine, Assistant Public Defender 

Public Defender’s Office 

Sitka, Alaska

Michael J. Rafferty, Chief of Forensics 

Florida Department of Law Enforcement

Fort Myers Regional Operations Center

Fort Myers, Florida

Eugene Rifenburg, Senior Investigator (Ret.) 

New York State Police Investigator (current) 

Oneida Nation Police 

Munnsville, New York

Gary A. Rini, Police Commander (Ret.) 

Director

The American Institute for Police Science

Elkhorn, Nebraska 

Heidi Robbins, Supervising Criminalist 

Scientific Services Bureau

Los Angeles County Sheriff’s Department 

Los Angeles, California

Darrell Ryan, Lieutenant 

Nashville Police Department

Nashville, Tennessee

Norman Shapiro, Vice President

New York State Defender’s Association 

Counselor at Law 

Law Offices of Norman Shapiro

Middletown, New York 

Clarene Shelley, Lieutenant 

Lakewood Police Department

Lakewood, Colorado 

Gregory Smith, Assistant County Prosecutor 

Office of the County Prosecutor 

Camden County

Camden, New Jersey

Richard Stanek, Captain

Minneapolis Police Department 

Minneapolis, Minnesota 

Brad Townsend, Sergeant

Corona Police Department

Corona, California 

Larry Turner, Director of Forensic Services

Jackson Police Department Crime Laboratory

Jackson, Mississippi

Stephen Weichman, County and Prosecuting 

Attorney 

Teton County 

Jackson, Wyoming 

James Wiser, Crime Scene Investigator/ 

Evidence Custodian 

Mount Pleasant Police Department 

Mount Pleasant, South Carolina 

Larry Wood, Detective

Major Case Unit

Smyrna Police Department

Smyrna, Georgia

John Yarbrough, Sergeant Homicide Bureau

Los Angeles County Sheriff’s Department 

Commerce, California
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Review Committee

Gregory B. Bailey, Evidence Technician 

(Ret.) 

Contract Assessor, Forensic Quality Services

Berkley, Michigan

Detective Brian Cochran, Teaching 

Associate

National Forensic Academy 

Boone County, Kentucky 

Robert C. Gaffney, CSCSA

Special Agent/Operations Officer

Forensic Research and Training Division 

US Army Criminal Investigation Laboratory 

Forest Park, Georgia

Lesley Hammer, D-ABC, CSCSA, CFWE

Forensic Examiner

Hammer Forensics

Anchorage, Alaska

Joseph Heppler, Computer Forensic Team 

Lead

CACI Contractor for U.S. Dept of State 

Computer Investigations and Forensics 

Arlington, Virginia

Mike James, Assistant Sheriff (Retired) 

Orange County Sheriff's Department 

Orange County, California

Gregory S. Klees, Firearms and Toolmark 

Examiner

Bureau of Alcohol, Tobacco, Firearms and 

Explosives

Ammendale, Maryland 

Sgt. Jim Markey, M.Ed

Consultant/Trainer 

Investigative Lead, LLC

Fountain Hills, AZ

Cory Latham, CSCSA, CBPE

Teaching Associate

National Forensic Academy 

Oak Ridge, Tennessee

Wayne Moorehead, F-ABC, F-AAFS

Forensic Consultant 

forensicTRACE

Rancho Santa Margarita, California 

Steve O'Dell, CSCSA, CBPE 

Senior Police Advisor 

Senior Forensic Laboratory Management 

Advisor 

International Narcotics and Law Enforcement

US Department of State

Washington, DC 

Joseph L. Parker, D-ABFDE

Forensic Document Examiner 

Atlanta Forensic Document Examiner 

Services, Inc.

Peachtree City, Georgia

Rodney A. Schenck, CLPE, CFWE, CCSA 

Physical Scientist, Forensic Latent Print 

Examiner 

U.S. Army Criminal Investigation Laboratory 

Expeditionary Forensic Division 

Forest Park, Georgia

Elizabeth Thompson, DNA Laboratory 

Director

Orange County Crime Laboratory 

Orange County Sheriff-Coroner 

Orange County, California
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Crime Scene Investigation A Guide for Law Enforcement

Section A Arriving at the Scene: Initial 

Response/Prioritization of Efforts 

Section B Preliminary Documentation and 

Evaluation of the Scene 

Section C Processing the Scene 

Section D Completing and Recording the 

Crime Scene Investigation 

Section E Crime Scene Equipment 

Authorization: Actions taken pursuant to this guide shall be 

performed in accordance with department policies and procedures and

Federal and State laws.
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This handbook is intended as a guide to recommended practices for 

crime scene investigation.

Jurisdictional, logistical, or legal conditions may preclude the use of 

particular procedures contained herein.

For potentially devastating situations, such as biological weapons or 

radiological or chemical threats, the appropriate agencies should be 

contacted. The user should refer to the National Institute of Justice’s 

publications for fire and arson investigation, bomb and explosives 

investigation, electronic crime investigation, and death investigation 

where applicable.
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Arriving at the Scene: Initial 

Response/ Prioritization of 

Efforts 

Note: Words and phrases that are defined in the glossary appear 

in bold italics on their first appearance in the body of the Guide.

1. Initial Response/ Receipt of

Information 

Principle: One of the most important aspects of securing the 

crime scene is to preserve the scene with minimal contamination

and disturbance of physical evidence. The initial response to an 

incident should be expeditious and methodical. 

Policy: The initial responding officer(s), upon arrival, shall assess 

the scene and treat the incident as a crime scene. They shall promptly, 

yet cautiously, approach and enter the crime scene, remaining 

observant of any persons, vehicles, events, potential evidence, and 

environmental conditions. 

Procedure: The initial responding officer(s) should:

a. Note or log dispatch information (e.g., address/location, time, 

date, type of call, parties involved).

b. Be aware of any persons or vehicles leaving the crime scene.

c. Approach the scene cautiously, scan the entire area to 

thoroughly assess the scene, and note any possible secondary 

crime scenes. 

d. Be aware of any persons and vehicles in the vicinity that may be 

related to the crime.

e. Make initial observations (look, listen, smell) to assess the scene 

and ensure officer safety before proceeding.

f. Remain alert and attentive. Assume the crime is ongoing until 

determined to be otherwise.

g. Treat the location as a crime scene until assessed and determined 

to be otherwise. 

h. Safely direct additional responding units into the area. 

A.
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Summary: It is important for the initial responding officer(s) to 

be observant when approaching, entering, and exiting a crime scene.

2. Safety Procedures

Principle: The safety and physical well-being of officers and other 

individuals, in and around the crime scene, are the initial responding 

officer(s’) first priority.

Policy: The initial responding officer(s) arriving at the scene shall 

identify and control any dangerous situations or persons. 

Procedure: The initial responding officer(s) should: 

a. Ensure that there is no immediate threat to other responders; 

scan area for sights, sounds, and smells that may present danger 

to personnel (e.g., hazardous materials such as gasoline, natural 

gas). If the situation involves a clandestine drug laboratory, 

biological weapons, or radiological or chemical threats the 

appropriate personnel/agency should be contacted prior to 

entering the scene.

b. Approach the scene in a manner designed to reduce risk of harm 

to officer(s) while maximizing the safety of victims, witnesses, 

and others in the area. 

c. Survey the scene for dangerous persons and control the 

situation. 

d. Notify supervisory personnel and call for assistance/backup. 

Summary: The control of physical threats will ensure the safety 

of officers and others present. 

3. Emergency Care

Principle: After controlling any dangerous situations or persons, 

the initial responding officer(s’) next responsibility is to ensure that 

medical attention is provided to injured persons while minimizing 

contamination of the scene. 

Policy: The initial responding officer(s) shall ensure that medical 

attention is provided with minimal contamination of the scene.

Procedure: The initial responding officer(s) should: 
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a. Assess the victim(s) for signs of life and medical needs and 

provide immediate medical attention. 

b. Call for medical personnel. 

c. Guide medical personnel to the victim to minimize 

contamination/alteration of the crime scene.

d. Point out potential physical evidence to medical personnel, 

instruct them to minimize contact with such evidence (e.g., 

ensure that medical personnel preserve all clothing and personal 

effects without cutting through bullet holes, knife tears), and 

document movement of persons or items by medical personnel. 

e. Instruct medical personnel not to “clean up” the scene and to 

avoid removal or alteration of items originating from the scene.

f. If medical personnel arrived first, obtain the name, unit, and 

telephone number of attending personnel, and the name and 

location of the medical facility where the victim is to be taken.

g. If there is a chance the victim may die, attempt to obtain “dying 

declaration.”

In some instances, fingerprint and shoe impressions of medical 

personnel may need to be taken for elimination purposes.

h. Document any statements/comments made by victims, suspects, 

or witnesses at the scene.

i. If the victim or suspect is transported to a medical facility, send 

a law enforcement official with the victim or suspect to 

document any comments made and preserve evidence. (If no 

officers are available to accompany the victim/suspect, stay at 

the scene and request medical personnel to preserve evidence 

and document any comments made by the victim or suspect.)

j.

Summary: Assisting, guiding, and instructing medical personnel 

during the care and removal of injured persons will diminish the risk 

of contamination and loss of evidence. 

Safeguard evidence, such as a weapon, that is taken into custody. 

Follow chain-of-custody procedures as soon as the evidence is 

confiscated.

4. Secure and Control Persons at the 

Scene

Principle: Controlling, identifying and removing persons at the 

crime scene, and limiting the number of persons who enter the crime 
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scene and the movement of such persons is an important function of 

the initial responding officer(s) in protecting the crime scene. 

Policy: The initial responding officer(s) shall identify persons at the 

crime scene and control their movement.

Procedure: The initial responding officer(s) should: 

a. Control all individuals at the scene—prevent individuals from 

altering/destroying physical evidence by restricting movement, 

location and activity while ensuring and maintaining safety at the 

scene.

b. Identify all individuals at the scene, such as:

• Suspects: Secure and separate.

• Witnesses: Secure and separate.

• Bystanders: Determine whether witness, if so treat as 

above; if not, remove from the scene. 

• Victims/family/friends: Control while showing 

compassion. 

• Law Enforcement, Medical and other assisting personnel. 

c. Exclude unauthorized and nonessential personnel from the 

scene (e.g., law enforcement officials not working the case, 

politicians, media).

Summary: Controlling the movement of persons at the crime 

scene and limiting the number of persons who enter the crime scene 

is essential to maintaining scene integrity, safeguarding evidence and 

minimizing contamination. 

5.Boundaries: Identify, Establish, 

Protect and Secure

Principle: Defining and controlling boundaries provide a means 

for protecting and securing the crime scene(s). The number of crime 

scenes and their boundaries are determined by their location(s) and 

the type of crime. Boundaries are established beyond the initial scope 

of the crime scene(s) with the understanding that the boundaries can 

be reduced in size if necessary but cannot be as easily expanded.

Policy: The initial responding officer(s) at the scene shall conduct 

an initial assessment of the extent of the crime scene(s) and then 

establish and control its boundaries.
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Procedure: The initial responding officer(s) should: 

a. Establish boundaries of the scene(s), starting at the focal point 

and extending outward to include: 

• Where the crime occurred.

• Potential points and paths of exit and entry of suspects and 

witnesses.

• Places where the victim/evidence may have been moved

(be aware of trace and impression evidence while 

assessing the scene).

b. Secure the scene. Set up physical barriers (e.g., ropes, cones, 

crime scene barrier tape, available vehicles, personnel, other 

equipment) or use existing boundaries (e.g., doors, walls, gates). 

c. Document the entry/exit of all people entering and leaving the 

scene, once boundaries have been established. 

d. Protect the scene. Control the flow of personnel and animals 

entering and leaving the scene to maintain integrity of the scene.

e. Institute measures to preserve/protect evidence that may be lost 

or compromised (e.g., protect from the elements (rain, snow, 

wind) and from footsteps, tire tracks, sprinklers). 

f. Document the original location of the victim or any objects that 

you observe being moved. 

g. Consider search and seizure issues to determine the necessity of 

obtaining consent to search and/or obtaining a search warrant. 

Note: Persons should NOT smoke, chew tobacco, use the telephone 

or bathroom, eat or drink, move any items from the scene including 

weapons (unless necessary for the safety and well-being of persons at 

the scene), adjust the thermostat or open windows or doors (maintain 

scene as found), touch anything unnecessarily (note and document 

any items moved), reposition moved items, litter, or spit within the 

established boundaries of the scene. 

Summary: Establishing boundaries is a critical aspect in 

controlling the integrity of evidentiary material.

Do not allow suspect to use 

bathroom facilities, or to alter his/her appearance, including brushing 

hair or washing hands. 
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6. Turn Over Control of the Scene and 

Brief Investigator(s) in Charge

Principle: Briefing the investigator(s) taking charge assists in 

controlling the crime scene, helps establish further investigative 

responsibilities and assists with the managing of resources.

Policy: The initial responding officer(s) at the scene shall provide a 

detailed crime scene briefing to the investigator(s) in charge of the 

scene.

Procedure: The initial responding officer(s) should: 

a. Brief the investigator(s) taking charge. 

b. Assist in controlling the scene. 

c. Turn over responsibility for the documentation of entry/exit. 

d. Remain at the scene until relieved of duty.

Summary: The scene briefing is the only opportunity for the next 

in command to obtain initial aspects of the crime scene prior to 

subsequent investigation. 

7.Document Actions and Observations

Principle: All activities conducted and observations made at the 

crime scene must be documented as soon as possible after the event 

to preserve information. 

Policy: The initial responding officer(s) shall maintain 

documentation as a permanent record. 

Procedure: The initial responding officer(s) should document: 

a. Observations of the crime scene, including the location of 

persons and items within the crime scene and the appearance 

and condition of the scene upon arrival. 

b. Conditions upon arrival (e.g., lights on/off; shades up/down, 

open/closed; doors and windows open/closed; smells; ice, 

liquids; movable furniture; weather; temperature; and personal 

items.) 

c. Personal information from witnesses, victims, suspects and any 

statements or comments made.
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d. Their own actions and actions of others. 

Summary: The initial responding officer(s) at the crime scene 

must produce clear, concise, documented information encompassing 

his or her observations and actions. 

This documentation is vital in providing information to substantiate 

investigative considerations.

8. Establish a Command Post (Incident 

Command System) and Make 

Notifications

Principle: Setting up a location where crime scene investigation 

activities can be coordinated, media meetings can be held, and team 

meetings can occur is very valuable. This command post provides a 

central location for crime scene investigation activities and 

assessment of resources. The activities also relate to ensuring that 

other key investigative participants are told of the investigation and 

included in activities as needed. 

Policy: The investigator(s) in charge shall set up a location where 

crime scene investigation activities can be coordinated, media 

meetings can be held, and team meetings can occur.

Procedure: The investigator(s) in charge should: 

a. Set up a temporary command post in a location where media 

can take necessary photographs without jeopardizing the scene 

(and evidence) security.

b. Notify investigators or appropriate department(s) (such as 

Homicide) of information gathered at the crime scene. Discuss 

details of the scene during this step. 

c. Notify Communications Department (Dispatch) of phone 

numbers at the command post. 

d. Ask Communications Department (Dispatch) to notify 

surrounding agencies and send teletypes regionally and nationally 

when a suspect has fled the scene. These alerts should include a 

description of the suspect, vehicles involved and contact 

information for the person these agencies should contact if they 

locate the suspect.

e. Brief the supervisor as required.
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f. Verify that a command post is established. If not, make the 

necessary arrangements to correct the situation.

g. Debrief with first responder and officer(s)/investigator(s).

h. Make necessary assignments, recording each on a formal 

assignment sheet.

i. Use assignment sheet to record assignment updates throughout 

the investigation. Make this assignment sheet available to 

personnel working on the case. Assign evidence recorder, 

entry/exit recorder (who is also responsible for keeping event 

timetable).

j. Ensure that the Communications Department is aware of 

important contact phone numbers including the command post. 

k. Establish the status and locations of victims and suspects. 

l. Establish the status of bulletins that have been broadcast 

regarding victims and suspects. Ensure that missing suspect 

alerts are broadcast. Establish a schedule for investigative team 

meetings (including all uniformed officers), during which status 

will be given, assignment updates will be made, and other key 

information will be shared. 

Summary: The establishment of a command post is critical to 

the communication among the crime scene responders, Dispatch and 

others providing information to the crime scene responders. 

9. Manage Witnesses

Principle: The timely interviewing of witnesses is crucial to the 

solution of a crime. Witnesses to crimes must be identified, secured, 

questioned at the scene, if applicable, and processed according to 

departmental regulations. 

Policy: The investigator(s) in charge shall identify and secure 

witnesses to crimes, interview them at the scene, if applicable, and 

process them according to departmental regulations. 

Procedure: The investigator(s) in charge should: 

a. Interview any witnesses at the scene separately to best use their 

reported experiences to benefit the overall investigation. Obtain 

written/recorded statements from each witness at the police 

station.

b. Transport each witness to the police station separately from 

other witnesses or suspects.
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c. When possible, the following tasks should be performed by the 

Supervising Officer:

• Establish the status and locations of each victim and 

suspect.

• Establish the status of bulletins that have been broadcast 

regarding each victim and suspect. Ensure that any 

necessary missing suspect alert is broadcast in a timely 

manner. 

Summary: The timely separate interviewing of witnesses is 

important to obtain information about any crime.
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Preliminary Documentation 

and Evaluation of the Scene

1.Conduct Scene Assessment

Principle: Assessment of the scene by the investigator(s) in 

charge allows for the determination of the type of incident to be 

investigated and the level of investigation to be conducted. 

Policy: The investigator(s) in charge shall identify specific 

responsibilities, share preliminary information, and develop 

investigative plans in accordance with departmental policy and local, 

State, and Federal laws.

Procedure: The investigator(s) in charge should: 

a. Converse with the first responder(s) regarding 

observations/activities.

b. Evaluate safety issues that may affect all personnel entering the 

scene(s) (e.g., blood-borne pathogens, hazards). 

c. Evaluate search and seizure issues to determine the necessity of 

obtaining consent to search and/or obtain a search warrant. 

d. Evaluate and establish a path of entry/exit to the scene to be 

utilized by authorized personnel. 

e. Evaluate initial scene boundaries. 

f. Determine the number/size of scene(s) and prioritize. 

g. Establish a secure area within close proximity to the scene(s) for 

the purpose of consultation and equipment staging. 

h. If multiple scenes exist, establish and maintain communication 

with personnel at those locations. 

i. Establish a secure area for temporary evidence storage in 

accordance with rules of evidence/chain of custody. 

j. Determine and request additional investigative resources as 

required (e.g., personnel/specialized units, legal consultation/ 

prosecutors, equipment). 

k. Ensure continued scene integrity (e.g., document entry/exit of 

authorized personnel, prevent unauthorized access to the scene). 

B.
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l. Ensure that witnesses to the incident are identified and separated

(e.g., obtain valid ID).

m. Ensure the surrounding area is canvassed and the results are 

documented. 

n. Ensure preliminary documentation/photography of the scene, 

injured persons and vehicles. 

Summary: Scene assessment allows for the development of a 

plan for the coordinated identification, collection, and preservation 

of physical evidence and identification of witnesses. It also allows for 

the exchange of information among law enforcement personnel and 

the development of investigative strategies.

2.Conduct Scene “Walk-Through” and 

Initial Documentation

Principle: The scene “walk-through” provides an overview of 

the entire scene, identifies any threats to scene integrity, and ensures 

protection of physical evidence. Written and photographic 

documentation provides a permanent record. A “walk-through” 

should only be completed if there will be no disturbing of evidence. 

There may be the need for the immediate documentation and 

collection of evidence prior to the walk through. 

Policy: The investigator(s) in charge shall conduct a walk-through 

of the scene. The walk-through shall be conducted with individuals 

responsible for processing the scene.

Procedure: During the scene walk-through, the investigator(s) in 

charge should: 

a. Avoid contaminating the scene by using the established path of 

entry. 

b. Consider whether personal protective equipment (PPE) should 

be used. 

c. Prepare preliminary documentation (e.g. notes, rough sketches) 

of the scene as observed.

d. Identify and protect fragile and/or perishable evidence (e.g., 

consider climatic conditions, crowds/hostile environment). 

Ensure that all evidence that may be compromised is 

immediately documented, photographed and collected. 
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e. When involved in the initial walkthrough, note the condition of 

the scene. Record relevant observations, which may include 

things such as: 

• Ceilings

• Doors, including entry and exit points: Are they open, 

closed, locked or forced open? On which side was the key?

• Windows: Are they open or closed? Is there broken glass? 

Were they locked or forced open? 

• Lights: On or off? If left on, which lights were on? 

• Shades or shutters: Open or closed? 

• Floors/Rugs 

• Interior lighting conditions 

• Odors: Cigarette smoke, gas, powder, perfume, etc.

• Description of perpetrator (when present) 

• Description of crime-related people present

• Description of emergency medical or search-and-rescue 

personnel present 

• Weapons observed 

• Furniture present, including location relative to victim, as 

applicable and overall scene 

• Signs of activity: Meal preparation, dishes in sink, condition 

of housekeeping (clean, dirty or items in disarray), 

appliances left on, television/stereo left on (note the 

channel), etc.

• Date and time indicators: Mail, newspapers, dates on milk 

cartons, stopped clocks, spoiled foods, items that should 

have been hot or cold, but are at room temperature 

• Temperature of the room and environmental conditions 

f. Develop a general theory of the crime

Summary: Conducting a scene walk-through provides the 

investigator(s) in charge with an overview of the entire scene. The 

walk-through provides the first opportunity to identify valuable 

and/or fragile evidence and determine initial investigative procedures, 

providing for a systematic examination and documentation of the 

scene. Written and photographic documentation records the 

condition of the scene as first observed, providing a permanent 

record.
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3.Note-Taking and Logs

Principle: Note-taking and logs provide a permanent record of 

crime scene activities.

Policy: All personnel assigned to the crime scene investigation shall 

maintain notes and logs of their activities. 

Procedure: Detailed entry/exit logs should be created. An 

entry/exit log is used to document the people who come to and go 

from a crime scene during the investigation. People who were at the 

crime scene before the investigation began are also noted in this log. 

a. The officer monitoring the log, the “Log Officer,” is assigned 

the task by the Supervising Officer and is responsible for 

completing this task and monitoring the log at all times. The Log 

Officer is responsible for ensuring that the log is filled out 

thoroughly and anyone entering the scene has a stated purpose 

there.

b. Position the log so that it is clearly visible. Set up the log for 

people to use when arriving to and departing from the scene. 

Record the following information about the crime scene: 

• Crime scene location

• Name of witnesses

• Name of victims

• Name of persons taken into custody 

• Name of first responders and approximate arrival times

• Name of Supervising Officer and approximate arrival time 

(approximate time should be used if arrival time was before 

the log was established)

c. Record the information below for each person at the scene. If 

not using an official log book or forms, leave spaces where this 

information can be recorded: 

• Arrival date

• Time of arrival

• Name

• Identification and Unit numbers 

• Organization (if not with the investigating department)

• Reason for being at the scene 

Log information should include: 
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• The arrival and departure times of all personnel at the crime 

scene, including the Coroner or Medical Examiner, crime 

scene technicians, and State's Attorney.

• Information about: who is at the crime scene and why they 

are there; incident number; first responder names, Log 

Officer and Supervising Officer names, shield numbers, 

Unit numbers; location of crime scene; name of victim(s) 

• Before making it available to crime scene visitors, record 

logistical data (time, crime scene location, names of victims 

and witnesses, etc.) in the entry/exit log.

 Ensure that the departure time for any person departing from 

the scene is recorded prior to that person actually leaving. 

 If someone exits the scene without reporting to the Log Officer, 

that officer can enter an estimated departure time along with a 

note stating the rationale for it being estimated. 

 Store the log in a secure location and as mandated by 

departmental regulations. 

Figure B-1. Sample crime scene entry log (see Appendix C-6 for 

full size document) 

Summary: Note-taking and an entry/exit log records the persons 

present at a crime scene for investigative and prosecutorial purposes. 
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Processing the Scene 

1.Determine Team Composition

Principle: It is essential to a successful investigation to select a 

team of trained personnel to perform scene processing, based on the 

type of incident and complexity of the scene. 

Policy: The investigator(s) in charge shall assess the scene to 

determine team composition and specialized resources required. 

Procedure: Following the walk-through, the investigator(s) in 

charge should: 

a. Assess the need for additional personnel. They should be aware 

of the need for additional personnel in cases involving multiple 

scenes, multiple victims, numerous witnesses or unique 

circumstances.

b. Assess forensic needs and call forensic specialists to the scene 

for expertise and/or equipment. 

c. Ensure that scene security and the entry/exit documentation are 

continued. 

d. Select qualified person(s) to perform specialized tasks (e.g., 

photography, sketch, latent prints, evidence collection).

e. Document team members and assignments.

Summary: The assessment of the scene(s) determines the 

number of personnel and how responsibilities will be assigned. 

2. Ensure Contamination Control

Principle: Contamination control and preventing crosscontamination at single or multiple scenes is essential to maintaining 

the safety of personnel and the integrity of evidence. 

Policy: The investigator(s) in charge shall require all personnel to 

follow procedures to ensure scene safety and evidence integrity.

Procedure: Other responders and/or team members should: 

C.
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a. Limit scene access to people directly involved in scene 

processing. 

b. Follow established entry/exit routes at the scene.

c. Identify first responders and consider collection of elimination 

samples. 

d. Designate a secure area for trash and equipment.

e. Use personal protective equipment (PPE) to prevent 

contamination of personnel and minimize scene contamination. 

f. Clean/sanitize or dispose of tools/equipment and personal 

protective equipment between each item of evidence collection 

and/or scenes. 

g. Utilize single-use equipment when performing direct 

collection of biological samples. 

Summary: Minimize contamination by being safe, clean and 

careful to ensure the welfare of personnel and the integrity of the 

evidence.

3.Documentation

Principle: An assessment of the scene determines what kind of 

documentation is needed (e.g., photography, video, sketches, 

measurements, notes).

Policy: The investigator(s) in charge shall ensure documentation of 

the scene.

Procedure: The team member(s) should:

a. Review the assessment of the scene to determine the type of 

documentation needed. 

b. Coordinate photographs, video, sketches, measurements and 

notes. 

c. Photograph (see Photography section for details): 

• Scene utilizing overall, medium, and close-up coverage. 

• Evidence to be collected with and without measurement

scale and/or evidence identifiers. 

• Victims, suspects, witnesses, crowd and vehicles.

• Additional perspectives (e.g., aerial photographs, witness’s 

view, area under body once body is removed). 
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d. Record video as an optional supplement to photographs 

e. Prepare preliminary sketch(es) and measure: 

• Immediate area of the scene, noting case identifiers and 

indicating north on the sketch. 

• Relative location of items of evidence, correlating evidence 

items with evidence records.

• Evidence prior to movement. 

• Rooms, furniture or other objects. 

• Distance to adjacent buildings or other landmarks. 

f. Generate notes at the scene: 

• Document location of the scene, time of arrival and time of 

departure. 

• Describe the scene as it appears.

• Record transient evidence (e.g., smells, sounds, sights)

and conditions (e.g., temperature, weather).

• Document circumstances that require departure from usual 

procedures. 

Sketching 

Equipment Needed 

• Graph paper 

• Paper

• 50- to 100-foot retractable measuring tape 

• 1000-foot walking wheel

• Folding rule 

• Ruler 

• Oversize clipboard with storage pocket

• Eraser

• Magnetic compass

• Personal protective equipment (when needed)

• Flashlight

• Notebook 

• Pencil

The accuracy of all measuring devices should be ensured by 

comparison to a measure of certified accuracy, such as a NIST 

traceable ruler.
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General Considerations 

 A sketch of a crime scene is required when spatial relationships 

or proportional measurements are needed. Use spatial 

relationships to relate evidence to other objects. Use 

proportional measurements to calculate such things as bullet 

trajectory angles or to reconstruct accident details.

 The rough sketch is the first sketch drawn at the scene; multiple 

rough sketches may be required depending on the crime. The 

sketch includes a scene outline with the location of objects and 

evidence clearly marked. A finished sketch is derived from the 

rough sketch. 

 Draw the rough sketch before anything is moved or destroyed, 

and after photographs are taken. Do not alter the scene.

 Depending on the crime, draw one or all of these types of 

sketches: a sketch showing the surrounding areas, a sketch 

showing only measurements, and a sketch showing locations of 

objects, such as the locations of evidence, victim(s), etc.

 Measurements should be accurate to within 1⁄4”. 

 Include, outside of the drawn crime scene, measurements for 

dimensions of rooms, furniture, doors and windows, and 

distances between objects, entrances and exits, bodies and 

persons. Draw details, such as object size, proportionally in a 

rough sketch. 

 Take measurements from fixed location reference point, such as 

walls or curbs, or from stationary appliances. 

 Include as much information as possible in the sketch: streets, 

plants, entry and exit points, location of bullets and cartridges, 

etc.

 Do not alter a rough sketch after leaving the crime scene. If 

changes are required of the rough sketch, photocopy the original 

rough sketch to preserve its integrity. 

 Newer technology makes use of laser scanning devices to 

perform crime scene sketching. The employment of these 

devices should be reserved to investigators trained in their use. 

Information That Should be Documented 

• Record the time, date, name of the person who contacted 

the authorities, and incident information as soon as 

notification of a crime is received. These notes should be 

kept separate from the sketch.
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• Initial notes about the incident should answer the who, 

what, when, where, why and how questions. 

• Incident information includes: who reported the incident, 

when the incident was first reported, the crime scene 

location, a description of incident and participant names. 

• Gather information to use when sketching by talking to 

others at the scene. Record that information in the notes. 

• Questions that can provide valuable information include: 

− How did the victim or suspect arrive at or leave from 

the scene?

− How was the crime committed?

− Which items were handled?

− Which items were moved?

− Which items are broken or stained? 

• Have potentially flammable vapors been detected at the 

scene? (When potentially flammable conditions exist, take 

appropriate precautions.) 

• While sketching the scene, record related information in the 

notes. It is critical to use a systematic approach to notetaking while sketching to maintain a record of your 

activities and the order of sketches made. 

• Specify the changes made to a scene prior to sketching, 

such as when objects were moved or placards added. Note 

who made the changes and why they occurred.

Determining the Scale 

Determine the scale to use for all sketches. The usual scale for 

outdoor scenes is one inch equals twenty feet. The usual scale for 

indoor scenes is one-eighth inch equals one foot. Select which 

standard units of measurement will be used: metric (meters, 

centimeters) or English (feet, inches).

An important consideration when determining the scale is fitting the 

scene to the sketch paper. To calculate the scale:

1. Determine the longest measurement at the scene.

2. Divide this measurement by the longest measurement of 

the sketch paper. The resulting number establishes the 

largest measurement end of the scale.
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3. Often graph paper is used for scale drawings. When using 

graph paper, assign a specific number of squares to the 

measurement identified in Step 2.

 Use this method to establish other measurements by substituting 

the actual measurement in place of the longest measurement in 

Step 1.

 Create a title block on the graph paper being used for the sketch 

in the lower right corner of the paper. The title block typically 

includes: 

• Case number 

• Crime type

• Victim name

• Name and ID# of sketcher

• Name and ID# of person verifying measurements

• Location of sketch 

• Date completed

 Create a legend for the sketch on the graph paper used for the 

sketch. Each sketch must include a legend that is specific to it. A 

legend identifies: 

• North-facing direction (usually points to top of page) 

• Identification symbols used for information in sketch 

• Sketch ID#

• Scale used

 An accepted practice for assigning identification symbols is: 

• Use compass points to identify walls. 

• Use evidence numbers assigned to objects to identify them 

in the sketch. 

 The legend will be updated after drawing the sketch to ensure 

accuracy and completeness, and to include:

• Measurements for dimensions of rooms, furniture, doors 

and windows 

• Distances between objects, entrances and exits, bodies and 

persons 

Categories of Sketches 

There are four categories of sketches:
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Perspective − A perspective sketch contains a vanishing point and 

depicts objects of evidence as they would appear to the eye with 

reference to relative distance and depth. 

Figure C-1. Perspective sketch of vehicle homicide scene 

Projection − A projection sketch usually contains only one 

viewpoint and depicts objects on one plane. The overview sketch (of 

the horizontal plane) is the most common type of sketch and is 

usually done from a bird’s eye view; it shows the floor plan. Less 

common is the elevation sketch (of the vertical plane), which shows a 

side view typically of landscapes or buildings. 

Figure C-2. Projection sketch of bathroom 

   Cases

   Cases

   Possible
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One extrapolation of the projection sketch is the “Exploded” view 

sketch that contains more than one wall from one viewpoint. It 

combines the overview and elevation sketches.

Schematic − The schematic sketch is used when it is desirable to 

represent a sequence of events such as following the trajectory of a 

bullet through a crime scene location. 

Detailed − The detailed sketch is used when describing a small area 

that is not easily incorporated into the overall drawing due to the 

scale chosen for the rough or finished scale drawing. This is 

especially useful for large crime scenes.

Creating a Projection Sketch 

1. Determine the view to be shown in the sketch: overhead or 

exploded. 

− The overhead view shows a floor plan. This is the 

most frequently used view in sketches.

− The exploded view shows a floor plan with walls laid 

out flat. Objects on the floor and on walls, such as 

bullet holes or bloodstains, are shown in their relative 

positions in the exploded view sketch. 

2. Draw an outline that is to scale of the area of interest, 

including locations of approaches and accurate 

measurements of the perimeter. The size of the outline 

should fill as much of the paper as possible. 

3. Draw the rough sketch before anything is moved or 

destroyed, and after photographs are taken. Do not alter 

the scene. Show locations of windows and doors. Use a 

curved line to indicate the direction that each door opens. 

4. Use only the selected units of measurement. The sketch or 

accompanying notes should indicate where a measurement 

of an object was taken (e.g., middle of the object, nearcorner, far-corner, etc.). Measurements of bloodstains are 

often done on a metric scale (e.g., millimeters).

5. Whenever possible, have another officer or crime scene 

investigator observe measurements for confirmation 

purposes. 
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Figure C-3. Detailed sketch of handgun indicating areas of 

possible blood stains 

There are four measuring techniques used to obtain 

accurate measurements for the sketch: 

Triangulation Method − The triangulation method 

utilizes two fixed permanent objects within the crime scene. 

Measurements are taken from each fixed point to each 

piece of evidence.

 

Figure C-4. Triangulation method of measurement 

Rectangular Coordinate Method − The rectangular 

coordinate method is used when measuring the distance to 

an object from two mutually perpendicular objects, such as 

walls that meet at a 90-degree angle.

Possible 

blood stains
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Figure C-5. Rectangular coordinate method of measurement 

Polar Coordinate Method − The polar coordinate 

method is more appropriate for an outdoor scene in which 

only a single fixed or reference point is present. Measure 

both the distance and direction (angle) an object is from a 

known reference point. The angle can be measured with 

either a large protractor or an optical device such as a 

transit or a compass. The protractor technique with a 360-

degree protractor is useful for underwater scenes.

Transecting Baseline Coordinate Method − The 

transecting baseline coordinate method is used to measure 

items of evidence when there are numerous objects in the 

crime scene and other measuring techniques will not work. 

This is accomplished by laying a tape measure down so that 

it crosses the entire room or area to be measured. This first 

tape measure becomes the baseline for all other 

measurements in the crime scene. Measurements are then 

made perpendicularly from this tape by laying another tape 

measure at a 90-degree angle to the first tape and measuring 

out to the evidence. 
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Figure C-6. Transecting baseline coordinate method of 

measurement 

6. Take accurate measurements of the exact locations and 

relative positions of evidence using the triangulation 

method when there are at least two fixed points within the 

outlined area. 

7. Use triangulation indoors or outdoors; it is an especially 

good method to use in areas lacking straight lines. 

8. Take measurements from fixed locations, such as a wall or 

curb, or from a stationary appliance. Identify these 

locations in your legend. 

9. Measurements should be accurate to within 1⁄4 inch. 

10. Include height measurements to show how far off of the 

ground an object was found. 

11. When determining distance based on triangulation: 

a) Select two fixed points within the outlined area, such 

as walls, curbs, or street signs.

b) Draw a baseline between the two fixed points.

c) Select another object within the outlined area.

d) Measure the distance to that object from each of the 

baseline's fixed end points, creating a triangle.

12. Use a measuring tool to ensure accurate measurements are 

taken.

13. Take accurate measurements of the exact location and 

relative position of evidence using rectangular coordinates 

and the baseline method when: there are two known points 

or accurate measurements are needed for an object located 
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on or perpendicular to the line between those two points 

(the baseline).

14. Use the baseline method in outdoor areas that are 

irregularly shaped and where no natural baseline is present. 

This method is useful in situations such as scenes that 

occur in the desert or on farmland. 

15. Take measurements from fixed locations, such as a lamp 

post or curb, or from a stationary appliance. 

16. Measurements should be accurate to within 1⁄4 inch. 

17. Include height measurements to show how far off of the 

ground an object was found. 

18. When determining distance based on the transecting 

baseline coordinate method: 

a) Select two fixed points within (at the outer edges of) 

the outlined area, such as a kitchen appliance, a door, a 

window, a corner or wall (a wall is preferred).

b) Create the baseline by drawing a line between the two 

selected fixed points. 

c) Measure the length of the baseline.

d) Select an object within the outlined area.

e) When the object is on the baseline, measure from one 

of the fixed end-points to the object. 

f) When the object is not on the baseline, draw a straight 

line from the object at a 90-degree angle to the 

baseline.

g) Measure the length of the line drawn. 

h) Measure from one of the fixed end-points to the point 

where the new line meets the baseline.

19. When a sketch is complete, prominently write “Not to 

Scale” outside of the sketch, then update related 

documentation, such as the legend and notes. Note: 

Although accurate measurements were taken, potential 

courtroom controversies related to those measurements 

may be avoided by placing the “Not to Scale” disclaimer on 

the rough sketch. 

20. Ensure that all identification symbols used on the sketch 

are included and defined on the legend. 

21. Include descriptive details related to the sketch in notes 

such as lighting conditions, names of people in the area, 

colors of objects, odors, weather. 
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22. Include updates such as distances between objects and 

dimensions of rooms, windows, doors, etc. 

23. Until all rough sketches for a crime scene are complete, 

repeat the previous steps. 

24. Do not update any rough sketch after leaving the scene. 

25. Finish note-taking at the scene. Include the time that 

sketching was completed in the notes. Note-taking should 

occur throughout the sketching activities. 

26. Finished sketches can be completed either by the originator 

of the rough sketches or by another staff member, such as a 

draftsperson or artist.

Photography 

Common crime scene investigation photography is an important part 

of the documentation of a crime scene. Photographs are typically 

taken based upon the perspective of the camera to the target.

a. Overall 

b. Midrange

c. Close-up 

Note: Videography is discussed on page 35. 

Equipment Needed 

• Personal protective equipment 

• Camera: 35mm and/or digital single-lens reflex (SLR) of 12 

megapixels or greater recommended (a backup camera is 

also advisable)

• Several rolls of color film and black-and-white film, if using 

film camera

• Battery backups

• Memory cards for digital cameras 

• Detachable flash or additional lighting 

• Flashlight

• Tripod 

• Camera cleaning supplies 

• Pen

• Notebook 

• Evidence placards

• Rulers

Courtesy of Scott Campbell
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General Considerations 

 Always use the designated safe route when moving through the 

scene. Avoid disturbing the scene. 

 When it is necessary to alter the scene, such as by placing 

placards or disassembling equipment, always take photographs 

of the scene before and after alteration, and with scale when 

appropriate. 

 When using a digital camera, never delete a photograph from 

camera or digital media memory. When using film, never discard 

used film or negatives. 

 Use a sturdy tripod with a cable release or the camera timer 

feature when placing the camera 90 degrees to the subject, such 

as when taking fingerprint/footprint/shoeprint/tire track 

photos. 

 Take interior photographs using a vertical orientation to get the 

full length of a wall in a photograph. 

 Take a complete set of pictures, including overall (long-range), 

midrange and close-ups. 

 Remove the film or download the digital images and store in a 

secure location according to departmental regulations when 

photography is completed.

Preliminary Steps 

1. Upon arrival at the scene, record names and arrival times 

(when known) of all personnel involved, including 

investigators, medics, first responders, etc.

2. When applicable, include the names of those assigned to 

specific tasks in either the photo log or, when using a photo 

list, a notebook. 

3. Plan the photography route. Photograph transient objects, 

such as bloodstains or latent prints, as soon as possible. 

Move from the exterior to the interior of the crime scene, 

and from general to specific focus.

4. The photography session should occur in an uninterrupted, 

systematic, focused manner. When planning the route, ask: 

− How did the victim or suspect arrive at or leave from 

the scene?

− How was the crime committed?

− Which items were handled?

− Which items were moved?
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 Which items are broken or stained? 

 Have potentially flammable vapors been detected at 

the scene? 

Caution: Some photographic and flash equipment is 

flammable. When potentially flammable conditions exist, 

appropriate precautions must be taken. 

Note: Do not go beyond boundary markers to take 

photographs unless absolutely necessary. 

5. Plan and prepare lighting for each scene and camera angle. 

The following techniques are commonly employed: 

a) Front lighting places the camera, lens, and flash or 

light source, directly in front of the object to be 

photographed. It is often the most appropriate type of 

lighting to use at crime scenes. 

b) Side or oblique lighting places the camera directly in 

front of the object while the flash or light source is 

placed to the side of the object to be photographed. 

This can range from 45 to 90 degrees depending on 

the subject and the shadow detail observed. Oblique 

lighting produces the best results for three-dimensional 

(3D) objects and is recommended for the following: 

 To show details such as tool marks, surface 

irregularities or textures 

 To show vehicle accident damage 

 When photographing in closets or other small 

spaces 

 When photographing polished surfaces 

6. Control the use of lighting by manually changing the 

aperture, shutter speed settings and turning on/off flash 

settings. It is important to have a detachable flash, or, if the 

flash is not detachable, another light source. 

Film Photography 

Most agencies no longer use film for crime scene photography. If 

film is used, consider the following guidelines: 

 Plan the type of film to be used for a series of photographs 

when anticipating taking midrange then close-up photographs 

immediately after overall photographs. 
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 If it is anticipated that a change of film will be necessary, change 

the film before taking the overall photographs. 

 Black-and-white film: Use black-and-white film for close-up 

photographs of fingerprint/footprint/shoeprint/tire track 

evidence.

 Color film: Use color film for close-up photographs of 

bloodstains and other bodily fluids. 

Overall Photographs 

 Take overall photographs of the area surrounding the scene 

from its perimeter, from multiple locations and angles. Include 

exteriors of buildings, cars, both sides of entries and exits, and 

bystanders. 

Overall photographs should: 

• Encompass the entire large scene and be overlapping

• Represent a 360-degree perspective and include a landmark

• Include identifying marks, such as house number(s) or 

license plate(s)

 Overall photos may also encompass smaller “sub-scenes” that 

exist within the larger scene. For indoor scenes, consider 

photography from each corner of a room, looking into the 

room. 

 After the scene is photographed as found, additional 

photographs with placards, used as evidence identifiers, must be 

wholly visible in overall photographs when they are used, such 

as when midrange and close-ups will be taken of the scene. They 

must be placed in close proximity to the subject(s) of the 

photograph. 

 While taking photographs of a scene, record related information 

in notes. 

 It is critical to use a systematic approach to note-taking while 

taking photographs to maintain a record of your activities and 

the order and location of pictures as they are taken.

 Specify the changes you made to a scene while taking 

photographs, such as when a light was turned on or objects were 

moved. 

 Note-taking should occur throughout the photography session. 

Finish note-taking at the scene. Include the time that 

photographing began and was completed in the notes. 
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Midrange Photographs 

 Take midrange photographs before and after placing placards or 

rulers. It is important to take photographs that establish the 

relationships of objects or reference points in the scene. 

 Take close-up photographs immediately after taking the 

midrange photograph, when appropriate. 

 It may be appropriate to follow midrange photographs of a 

scene with close-up pictures of the same scene when showing a 

scene before, during, and after placard/ruler placements, nearviews of human injuries or vehicle accident damage.

 While taking photographs of a scene, record related information 

in notes. 

 Take photographs of transient evidence from a position that: 

• Shows the spatter relative to other objects in the area

• Is perpendicular to the spatter 

 Take pictures of the scene before and after placing the placards 

and rulers, and after removing the placards and rulers. 

Photographing the Deceased 

Before the body of a deceased person is moved, it should be 

photographed. The following guidelines should be followed: 

 Take photographs from all possible angles. Show a facial view, 

and the positions of the hands and feet when possible to do so 

without altering the body, its clothing or position. Wound 

photography should be conducted at close-up range. 

 Take photographs while moving around the body and from an 

overhead perspective. Photograph the body from two 

perspectives, when possible: 

a) As though looking at the body from a standing position 

b) From the same level as the body is lying, such as at ground 

level when the body is lying on the ground 

 Use oblique lighting to show wounds on the body, such as bite 

marks, with and without a scale.

 After the deceased has been removed from the scene, 

photograph the area where the body was. 

 Signs of activity can include: 

• TV and room lights turned on 

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• A glass holding a cold beverage (ice melting or still frozen) 

and a plate with fresh food on it 

• Scattered clothing, magazines, or other objects 

• A landline phone that was in use and is making a loud 

notification sound 

• Misplaced furniture, as with a tipped stool beside a body 

• Cigarettes, lit or remains piled in ashtray

• Tool marks in unusual location or near entry/exit 

• Shoeprints and/or fingerprints 

• Drug paraphernalia 

 Include the time that photographing was begun and completed 

in the notes. 

 Remove the film or download the digital images and store in a 

secure location according to departmental regulations. 

Close-up Photographs 

 Photograph fingerprint/footprint/shoeprint/tire track evidence 

using black-and-white film (when using film). Carefully place the 

ruler and camera perpendicular to each other and relative to the 

impression. 

 When the photograph needs to be accurately scaled: 

• The ruler must be on the same plane as the impression. 

• The camera lens must be perpendicular (90 degrees) to the 

subject. 

• Use a level and tripod to position the camera accurately.

 Take multiple shots with the light or flash at different angles, 

such as 0 degrees, 15 degrees, 45 degrees, etc., to achieve the 

best possible photographs. 

Taking Photos of Impression Evidence

Tire Impressions: For tire impressions, take a series of overlapping 

photographs showing the tire's entire circumference. 

Impressions on Glass: When the impression is on glass and when 

possible: 

• Protect latent prints

• Position a colored card or piece of cloth that contrasts with 

the impression behind the glass 

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• Include in notes that this approach was used for contrast 

purposes to obtain the photograph 

Impressions on a Mirror: When the impression is on a mirror, hold 

the flash to the side (oblique lighting); use a tripod to avoid being in 

the photograph. 

Dust Impressions: When photographing a dust impression or an 

impression in a soft material (e.g., wax or putty), use reflective 

lighting (also known as oblique lighting). When using reflective 

lighting, if detail does not appear sufficiently, block the ambient light 

and then experiment with positioning the light or flash in other 

locations until the desired result is achieved. 

Impressions on a Porous Surface: When the impression is on a 

porous surface, position the light or flash wherever the best results 

or contrast can be achieved, such as at a 90-degree angle from the 

impression. 

Photograph bloodstains or other bodily fluid stains using color film 

or digital camera. Carefully place the camera plane perpendicular to 

the plane of the stain and ruler. Stay alert to the location of the 

bloodstains, so equipment isn’t inadvertently touched to the stain. 

• It is extremely important that the ruler be on the same 

plane as the impression. 

• If the stain is on a wall, use an adhesive label with a ruler on 

it. Otherwise, tape a ruler beside the stain, or have an 

assistant hold the ruler beside the stain. Indicate upward 

direction. 

• Ensure that the camera lens is perpendicular (90 degrees) to 

the subject. Adjust lighting when photographing the stain 

to obtain the best contrast and result. 

• When the stain is on glass, position a colored card or piece 

of cloth that contrasts with the stain behind the glass, 

making sure to protect latent prints; include in the notes 

that this approach was used for contrast purposes to obtain 

the photograph. 

• When the stain is on a mirror, hold the flash off to the side 

(oblique lighting) and use a tripod to avoid being in the 

photograph. The camera will show in the photograph when 

the mirror is 90 degrees from the lens. 

Additional Techniques for Close-up Photography 

If not using a digital camera, photograph wounds using color film. 

Carefully place the camera perpendicular relative to the wounds to 

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obtain accurate measurements. Photograph the body of a deceased 

person before moving it and also photograph it at the morgue. 

Include scales where appropriate.

• Adjust lighting when photographing the wounds to obtain 

the best contrast and result. Take multiple shots with the 

light held or placed at different angles to the subject in 

order to achieve the best results.

• Retake photographs of wounds such as bruises at different 

intervals to capture changes, such as in color, over several 

days.

Photograph serial numbers on weapons or VIN numbers on vehicles: 

1. Carefully place the ruler, camera and placard relative to the 

item to obtain accurately scaled photographs. 

2. Place placard and ruler on the same plane as the weapon. It 

is extremely important, when the photo needs to be 

accurately scaled, that the ruler be on the same plane as the 

subject. The camera lens should be perpendicular (90 

degrees) to the subject.

3. Position the lighting to obtain the best possible contrast 

and results. Take multiple shots with the light held or 

placed at different angles to the subject in order to achieve 

the best results.

4. Photograph vehicular damaged areas, the license plate and 

the registration decal.

5. Include the time that photographing was begun and 

completed in the notes. 

6. Remove the film or download the digital images and store 

in a secure location according to departmental regulations. 

Aerial Photographs 

 Take aerial and/or overhead photographs of a scene to show 

geographic relationships of locations or objects and aid 

identification of objects shown in other photographs. 

 Obtain aerial photographs by taking the pictures from a 

helicopter or plane. News footage can sometimes be a useful 

source of aerial photographs. 

 Overhead photographs, in this context, are taken from above 

the scene, such as from a ladder, a second story, a cherry picker; 

they are not taken from the sky, as from a plane.

 Aerial and overhead photographs must be overlapping. 
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 Remove the film or download the digital images and store in a 

secure location according to departmental regulations. 

Videography 

Equipment Needed 

• Video camera

• Video kit with battery and recording media 

• Personal protective equipment 

• Additional sources of lighting 

• Camera cleaning supplies 

• Tripod 

• Pen

• Notebook 

General Considerations 

 Plan the video shoot carefully. Take video of the scene in its 

original state from multiple angles and distances. Take video of 

fragile evidence first.

 Avoid disturbing the scene. Always take video of the scene 

before and after alteration, such as when placards and scales are 

placed near evidence.

 Exclude officers, bystanders, and others at a scene from the 

video. Turn audio off. 

 Take overall (long-range) video to show where the crime 

occurred, midrange video to show relationships of evidence and 

other points of interest, and close-up video to show individual 

items and their characteristics.

 Use a sturdy tripod whenever possible to reduce movement 

while taking video. Take video from angles that result in the best 

representation of that scene. Avoid panning side to side or up 

and down. Avoid zooming while out of focus. 

 Always use the designated safe route when moving through the 

scene.

 When applicable, include the names of those assigned to specific 

tasks in your notebook. 

 Plan the videography route. Take video of transient objects, 

such as bloodstains or latent prints, as soon as possible. Move 

from the exterior to the interior of the crime scene, and from 

general to specific focus.
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 The videography session should occur in an uninterrupted, 

systematic, focused manner. When planning the route, ask: 

• How did the victim or suspect arrive at or leave from the 

scene?

• How was the crime committed?

• Which items were handled?

• Which items were moved?

• Which items are broken or stained? 

• Have potentially flammable vapors been detected at the 

scene?

Caution: Some equipment is flammable. When potentially 

flammable conditions exist, appropriate precautions must be 

taken.

 Go beyond boundary markers to take video only when 

necessary.

 Plan and prepare lighting for each scene and camera angle. 

a) Front lighting places the camera lens at a 90-degree angle to 

the recorded object. It is often the most appropriate type of 

lighting to use at crime scenes.

b) Side lighting places the lighting source at a 45-degree angle 

to an object. It is used: 

o to show details such as tool marks, surface 

irregularities or textures

o to show vehicle accident damage

o when videotaping in closets or other small spaces 

o when videotaping polished surfaces 

 Control the use of lighting by manually changing the focus 

settings and turning on/off flash settings. 

 It is important to have a detachable flash or, if the flash is not 

detachable, another light source. 

 Turn audio off. 

 Record overall video of the house/building exterior, vehicles, 

other structures at the crime scene, including entrances and 

exits, and bystanders. Slowly pan in one directional sweep; never 

move the camera side to side or up and down. 
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 Overall video should include a 360-degree view of the entire 

scene including landmarks, entrances and exits, and identifying 

marks, such as a house number or license plate. 

 Always use slow camera movements such as when panning and 

zooming. 

 Use a tripod whenever possible, unless using it will disturb either 

the scene or other team members.

 When recording a long, narrow area, such as a side yard or train 

tracks, use a tripod and slow zooming. Always avoid walking 

while taping these shots 

 Record entry/exit points from all possible angles. Show any 

paths used during the crime, when possible. 

 While taking video of a scene, record related information in 

notes. Specify any changes made to a scene while taking video, 

such as when a light was turned on or the tripod left a mark. 

 Film midrange and close-up exterior video (within 5 feet of 

subject) immediately following the overall recording of a scene. 

 Record in a systematic, focused way. 

When recording video: 

• Use slow camera movements such as when panning and 

zooming. Before zooming, stop filming, zoom, focus, then 

start the filming.

• Use the tripod whenever possible. 

• When recording a long, narrow area, such as a side yard or 

train tracks, use a tripod and slow zooming unless using it 

will disturb either the scene or other team members. Always 

avoid walking while recording a long, narrow area.

• A high camera angle, such as with an overhead view, may 

be required to show individual objects that are on similar 

planes. 

• Record entry/exit points from all possible angles. Show any 

paths used during the crime. 

 Move to the interior and take overall, midrange, and close-up 

video. When recording interiors: 

• Always use slow camera movements such as when panning 

and zooming. 
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• Use a tripod whenever possible, even though it takes more 

time to set up, unless using it will disturb either the scene or 

other team members.

• When recording overall video in tight spaces, such as closet 

or bathroom, use a high camera angle from a corner. 

• When recording a long, narrow area, such as a hallway or 

porch, use a tripod and slow zooming. Always avoid 

walking while recording these shots. 

• When necessary and possible, use artificial lighting to get 

the best possible clarity.

• Consider using a blue filter over artificial light to achieve 

similar lighting as daylight.

 Complete note-taking. Include in the notes such items as events 

that occurred while recording and the time recording was 

completed. 

 Remove the videotape from the camera or download the digital 

video before storing the camera in a secure location according to 

departmental regulations. 

Summary: A well-documented scene ensures the integrity of the 

investigation and provides a permanent record for later evaluation. 

4. Prioritize Collection of Evidence

Principle: The collection of evidence must be prioritized to 

prevent loss, destruction, or contamination. 

Policy: The investigator(s) in charge and team members shall 

determine the order in which evidence is collected.

Procedure: The team member(s) should:

a. Identify roles of the team members (e.g., scribe, collector, 

packager, etc.)

b. Conduct a careful and methodical evaluation considering all 

physical evidence possibilities (e.g., biological fluids, latent 

prints, trace evidence).

c. Focus first on the easily accessible areas in open view and 

proceed to out-of-view locations.
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d. Select a systematic search pattern for evidence collection based 

on the size and location of the scene(s). 

e. Select a progression of processing/collection methods so that 

initial techniques do not compromise subsequent processing/ 

collection methods. 

• Concentrate on the most transient evidence (e.g., most 

susceptible to environmental conditions) and work to the 

least transient forms of physical evidence.

• Move from least intrusive to most intrusive 

processing/collection methods. 

f. Continually assess environmental and other factors that may 

affect the evidence.

g. Be aware of multiple scenes (e.g., victims, suspects, vehicles, 

locations). Processing one scene at a time to avoids cross 

contaminating these various scenes

h. Recognize other methods that are available to locate, technically 

document, and collect evidence (e.g., alternate light source

enhancement, blood pattern documentation, projectile trajectory 

analysis).

Summary: Prioritization provides for the timely and methodical 

preservation and collection of evidence.

5.Crime Scene Search Methods

Principle: The thorough search of a crime scene helps ensure that 

all relevant evidence will be recognized, documented and collected. 

Policy: The investigator(s) in charge shall consider different search 

strategies for crime scenes depending upon locale and the number of 

officials available to aid in searching.

Procedure: There are four types of search methodology that can 

be considered to search a crime scene:

• Lane or strip search

• Grid search

• Zone Search

• Spiral search
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General Considerations 

 The size of the lane of the search should be approximately the 

arms’ length of the searcher.

 As the search of an area is completed, some marking should be 

made to indicate that the area has been completed.

 A mechanism should exist for the circumstance when potential 

evidence is found (e.g. who is called over, what path they should 

take, whether the other searches should halt moving until this 

finding is resolved). 

Lane or Strip Searches are accomplished by the searchers walking 

in parallel along defined lanes in the same direction. 

Figure C-7. Lane or strip search 

A Grid Search is a lane search that is conducted by completing a 

lane search in one direction and then completing a lane search in a 

perpendicular direction. While it takes twice as long as a lane search, 

it provides a more thorough search of an area. 

Figure C-8. Grid search 
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A Zone Search involves dividing the area to be searched into 

adjacent zones. The smaller the size of the zone, the more methodical 

the search can be. Zone searches may be done by multiple searchers 

per zone. 

Figure C-9. Zone search 

Spiral search involves a spiral into (inward) or out from (outward) a 

crime scene. A practical disadvantage with outward spiral searches is 

the evidence may be destroyed as the searchers move to the center of 

the crime scene area to begin their outward search.

Figure C-10. Spiral search (outward) 
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6.Collect, Preserve, Inventory, Package, 

Transport, and Submit Evidence

Principle: The handling of physical evidence is one of the most 

important factors of the investigation. 

Policy: The team member(s) shall ensure the effective collection, 

preservation, packaging, and transport of evidence. 

Procedure: The team member(s) should:

a. Maintain scene security throughout processing and until the 

scene is released. 

b. Document the collection of evidence by recording its location at 

the scene, date of collection, and who collected it. 

c. Collect each item identified as evidence.

d. Establish chain of custody. 

e. Obtain standard/reference samples from the scene.

f. Obtain control samples. 

g. Consider obtaining elimination samples. 

h. Immediately secure electronically recorded evidence (e.g., 

answering machine tapes, surveillance camera videotapes, 

computers) from the vicinity.

i. Identify and secure evidence in containers (e.g., label, date, initial 

container) at the crime scene. Different types of evidence require 

different containers (e.g., porous, nonporous, crush-proof). 

j. Package items to avoid contamination and cross-contamination. 

k. Document the condition of firearms/weapons prior to 

rendering them safe for transportation and submission. 

l. Avoid excessive handling of evidence after it is collected.

m. Maintain evidence at the scene in a manner designed to diminish 

degradation or loss. 

n. Transport and submit evidence items for secure storage. 

7.Detailed Crime Scene Evidence 

Collection
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Principle: The accurate and timely collection of evidence can lead 

to the reconstruction of a scene to determine if a crime has been 

committed, to the identification of suspect(s) and to the successful 

completion of the investigation. 

Policy: First responders, investigators and specialized personnel 

who are properly trained, equipped and prepared shall collect any and 

all potential evidence.

Procedure: Appropriate personnel with the required knowledge 

and training should collect evidence in each of the following evidence 

categories as applicable.

Ignitable Liquids 

Accelerants and ignitable liquids recognition and collection are best 

performed by specialized personnel. For scene personnel, evidence 

may be observed through smell, sight and sound, and should be 

recorded in notes. 

a. NEVER attempt to collect any evidence until you have spoken 

with an accelerants and ignitable liquids investigator or specialist.

b. Ensure the safety of people at or near the scene.

c. Follow the instructions provided by the accelerants and ignitable 

liquids investigator or specialist with whom you speak. 

Bodily Fluids 

Bodily fluids include blood, semen, urine and other physiological 

fluids. 

Blood 

Possible substrates with blood stains:

• Clothing 

• Entire portable object 

• Part of a non-portable object 

• Stain on a non-porous surface 

Equipment needed for bodily fluid collection includes:

Paper bags, boxes, and envelopes; cotton-tipped swabs; paper bindles 

or other sterile swab storage container; distilled water or one-time use 

sterile water; scalpel, utility knife, or scissors; clean paper; waterproof 

pen; evidence tape; protective gloves; face protection. Commercial 

products are also available for crime scene collection of stains
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Blood and other physiological fluids are fragile, and certain 

best practices must be maintained. 

a. Do not package bloodstained evidence in plastic bags. 

b. If possible, collect the entire stained garment.

c. Avoid altering the stain or transferring blood from one portion 

of the garment to another; do not fold or crumple the garment. 

d. Be careful not to lose or contaminate any remaining trace 

evidence on the garment. 

e. Avoid excessive heat when collecting, transporting or storing 

blood evidence. 

f. Avoid moisture, water or other liquids. 

g. Avoid exposing the bloody evidence to strong light, especially 

UV light.

h. Avoid touching, taking off gloves, or coughing/sneezing over or 

near the evidence.

i. Describe the stain as a “red stain” or “apparent bloodstain”. Do 

not label it as blood if the stain has not been forensically 

identified as such. 

j. Mark package with appropriate cautions about contents, such as 

“Store Frozen” or by affixing Biohazard stickers. 

Caution: Leaving evidence exposed at a crime scene can lead to 

contamination. It may not be possible to dry an item at the 

scene without risking contamination. 

Bodily Fluids Packaging Guidelines 

Documentation

Ensure that the portion of the area or object with the stain has been 

documented as it was found. When photographing the object: 

• Include a scale and an identification label.

• Take one or more location photographs that show the 

object where it was found. 

• Show the relationship of the object to other evidence in the 

photograph. 

Marking Evidence 

Evidence labeling: Label a container such as a paper bag or 

envelope with your initials and identification number, the date and 

time, case number, evidence number, location and evidence 

description. 
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Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description should include: 

• Type of item (e.g., victim’s shirt, glass, carpet fibers)

• Location of the stain 

• Whether the stain is wet or dry

• Location of the item at the crime scene

Drying Wet Material 

a. If the item is wet, place it on a clean piece of paper and allow it 

to dry before packaging, or transport it for drying at a laboratory 

facility or a properly outfitted evidence holding area. 

b. If you have access to a drying rack, dry the item in it. Place a 

clean piece of paper on the floor of the drying rack. Hang the 

item over the paper.

c. If you do not have a drying rack: 

1. Lay a clean piece of paper on a clean, flat surface in a secure 

location where the item will not be disturbed and 

contamination will be minimized.

2. Carefully place the item on the paper.

3. Be sure to keep the stain intact in its original form and 

avoid transferring the stain from one area of the item to 

another. 

4. Allow the item to dry naturally. Never expose it to heat, 

such as from a blow dryer. Avoid exposing the sample to 

direct sunlight. 

d. Do not place two items in the same container for drying 

purposes. Clean the surfaces of the drying rack with a 

disinfectant such as 10% bleach solution after the item has been 

dried and removed. 

Packaging

a. Collect and label the paper on which the object was dried. 

b. Place the paper into a labeled container, as needed. 

c. Carefully pick up and fold the paper on or over which the object 

was dried. Contain any trace evidence that may have fallen on 

the paper. 

d. Label the folded paper, indicating the evidence number of the 

item that was dried. 
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Storing Blood

Ideally, bloodstained items should be stored in a temperaturecontrolled environment (between 60-75 degrees, with less than 60% 

humidity). If stored at ambient temperature:

• Place the container in a secure, dry storage area.

• Never expose the container to extreme heat, such as from a 

heater vent.

• Avoid exposing the container to direct sunlight. 

Specific Items Procedure: On Clothing 

a. Label a container that will be used to collect the object. (See 

Bodily Fluids Packaging Guidelines) 

b. Document the location of the garment with photography, 

measurement and sketching, where appropriate. If wet, dry 

according to guidelines.

c. Fold garment and, whenever appropriate, wrap the garment in 

clean paper. 

d. When folding a garment or large object: 

1. Do not crumple or wad any portion of the garment. 

2. Fold the garment only enough so that it fits into the 

container. 

3. Do not crease the stained area.

4. Make sure, if using paper, that the paper protects trace 

evidence and prevents transferring the stain to other areas 

of the garment.

e. Only wrap an item if wrapping the object will not disturb the 

position of a stain or mark. 

f. An item should be wrapped in clean paper when the location or 

pattern of the stain or mark is significant (such as a handprint or 

spatter pattern).

g. Position the paper to keep the stain or mark intact in its original 

form. Avoid transferring any of the stain or mark to another 

portion of the object. 

h. Mark package with appropriate Biohazard cautions regarding 

contents. 

i. Place the item into the labeled container, such as a paper bag. 

The container should be large enough to allow air to circulate 

around the object inside of it. If an object is too large to be 

packaged in a container, protect the stained area(s) with clean 

paper during transport. 
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j. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

Specific Items Procedure: Entire Portable Object (e.g., a sheet) 

a. Label a container that will be used to collect the object. (See 

Bodily Fluids Packaging Guidelines.) 

b. Document the location of the object with photography, 

measurement and sketching, where appropriate. Dry, if wet, by 

placing it on or over a clean piece of paper and allowing it to dry 

before packaging; or dry in place. 

c. Whenever appropriate, wrap the object in clean paper. Only 

wrap an item when wrapping the object will not disturb the 

position of a stain or mark. 

d. Objects should be wrapped in clean paper when the: 

• Location or pattern of the stain or mark is significant (such 

as a handprint or spatter pattern). 

• Object is saturated and liquid will leak through the 

container if not wrapped. 

e. Position the paper to: 

• Keep the stain or mark intact in its original form. 

• Avoid transferring any of the stain or mark to another 

portion of the object. 

f. Place the object into the labeled container. If an object is too 

large to be packaged in a container, protect the stained area(s) 

with clean paper during transport. 

g. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

Specific Items Procedure: Part of a Non-portable Object (e.g., 

carpeting, car seat) 

a. Label a container that will be used to collect the object. (See 

Bodily Fluids Packaging Guidelines.) 

b. Document the location of the stain with photography, 

measurement and sketching, where appropriate. 

c. When multiple stains are found, take one or more photographs 

that show the relationship among those stains. 

d. It is important to collect the entire stained area if the shape of 

the stain is significant (such as a handprint). 
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e. If possible, cut out the entire stained area using a clean scalpel, 

utility knife, or scissors, including a large portion of the nonstained area. If the stain has been absorbed into multiple layers 

(such as carpet and carpet pad), collect a cut-out from each layer.

Figure C-11. Stained areas of carpeting are cut out for transport. 

f. If the entire stained area is too large to collect, cut out a smaller 

section of the area. 

g. Opposite the stained side, mark the orientation of the cut-out; 

for example, mark the area that pointed north, when collected. 

h. If the cut-out is wet, place it on clean paper and allow it to dry 

before packaging.

i. Whenever appropriate, wrap the object in clean paper if 

wrapping the object will not disturb the position of a stain or 

mark.

j. Objects should be wrapped in clean paper when the: 
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• Location or pattern of the stain or mark is significant (such 

as a handprint or spatter pattern). 

• Object is saturated and liquid will leak through the 

container if not wrapped. 

k. Position the paper to keep the stain or mark intact in its original 

form. Avoid transferring any of the stain or mark to another 

portion of the object. 

l. Control Sample: Label a second container with your initials and 

identification number, the date and time, evidence number, 

location of the control in relation to the original sample, and 

description of the control sample. 

m. Control sample number: Each piece of evidence, including the 

control sample, must have a unique number. A letter or number 

may be appended to the original evidence number to denote the 

control sample; e.g., If the original evidence number was #32, 

the control sample could be #32A or #32.1. 

n. Control sample description: The description should include: 

• Type of material

• Location of material

• Location of the control sample in relation to the stain

o. Collect a control sample: Cut out a portion of unstained 

material.

1. First, locate an unstained area of the same material from 

which the original sample was taken. Select the least 

contaminated area possible (such as an unstained area of 

carpet).

2. Cut out the control sample using a scalpel, utility knife, or 

scissors. (Use a clean blade; never use a blade that was used 

to cut another sample.) If multiple layers (such as carpet 

and carpet pad) of material were collected in the original 

sample, collect multiple layers for the control. 

3. On the side of the cut-out opposite the stained side (of the 

original non-control sample), mark the orientation of the 

cut-out to north when collected. 

4. If the cut-out is wet, place it on clean paper and allow it to 

dry before packaging. Package the control sample separately 

from the corresponding stained material. 

5. Place the cut-out in the container. Close the container and 

seal the entire opening with evidence tape. Write your 
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initials, identification number, and the date and time across 

the evidence tape seal.

Specific Items Procedure: Stain on a Non-porous Surface (e.g., 

glass, a door, concrete surface) 

a. Label a container that will be used to collect the item. (See 

Bodily Fluids Packaging Guidelines.) 

b. Document the location of the stain with photography, 

measurement and sketching, where appropriate. 

c. When multiple stains are found, take one or more photographs 

that show the relationship among those stains. 

d. Pre-label with distinguishing markings any swabs that you will 

use.

e. If the stain is dry, moisten the cotton tip of a swab using two or 

three drops of distilled water. If the stain has some residual 

moisture in it, touch the dry swab tip to the moist area of the 

stain. 

f. To avoid contamination, do not touch the cotton tip of the swab 

to any surface other than the sample area.

g. Hold the bottle of distilled water, or a one-time use vial of sterile 

water, above the swab. Use a minimum amount of water to 

moisten the swab: drop two or three drops of water onto the 

swab:

• Do not touch the tip of the water bottle to the swab. 

• Do not saturate the swab. (It should be moist, but not 

dripping wet.) 

h. Swab the stain with the cotton-tipped end of the swab. Touch 

the swab gently and firmly to the stain. Rotate the swab to 

ensure that the stain is collected on as much of the cotton tip as 

possible. Do not smear the stain when swabbing it. 

i. Dry the swab in a sterile container, swab dryer or drying box. If 

necessary, break off the end of the swab so it fits into the drying 

container. 

j. Place the swab into a bindle; fold the bindle so it seals around 

the swab. Close the bindle and place it into an envelope large 

enough to allow air to circulate around the object inside of it. If 

the swab is thoroughly dried, it can be placed directly into a prelabeled envelope.

k. If no bindle or swab drying box is available, use another sterile 

container that can hold the swab while it dries. Ensure that the 

swab is positioned so that air freely circulates around it. 
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l. Close the envelope or other container and seal the entire 

opening with evidence tape. Write your initials and identification 

number, and the date and time across the evidence tape seal.

m. Control sample: Label the second envelope with your initials 

and identification number, the date and time, evidence number, 

location of the control in relation to the original sample, and 

description of the control sample. 

n. Control sample number: Each piece of evidence, including the 

control sample, must have a unique number. A letter may be 

appended to the original evidence number to denote the control 

sample; e.g., If the original evidence number was #32, the 

control sample could be #32.A or #32.1. 

o. Control sample description: The description includes location 

of the control sample in relation to the original stain. 

p. Collect a control sample: Moisten the cotton tip of the second 

swab using two or three drops of distilled water. Swab an 

unstained area of the same surface from which the original swab 

was taken.

q. Dry the swab and package in the same manner as the stained 

sample.

Additional Physiological Fluid Types 

Semen 

a. Label a container that will be used to collect the item. (See 

Bodily Fluids Packaging Guidelines.) 

b. Document the location of the stain with photography, 

measurement and sketching, where appropriate. 

c. Locating semen stains: Unlike blood stains, semen stains are 

not always obvious to the unaided eye at a crime scene. Semen 

stains are difficult to see under room and ambient lighting 

conditions. They may appear as a slightly yellow stain on lightcolored fabrics or a whitish stain on dark-colored fabrics. Semen 

stains may also appear “crusty.” 

Still, many stains will be missed by normal or unaided visual 

examination; therefore, it is best to collect any item that may 

have semen stains. Common items to collect are:

• Victim’s clothing, especially underwear of sexual assault 

victims

• Suspect’s clothing 

• Bedding (e.g., blankets, sheets) where an alleged sexual 

assault took place 
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• Towels

• Tissue paper, car seats

d. Detecting semen stains: Items that are impractical to submit 

to the laboratory (e.g., vehicles, carpets) can be screened using 

special lighting techniques. 

All visual lighting techniques are screening tests that can fail to 

detect semen stains; detection varies depending on the type of 

fabric or material on which the stain may be deposited. Visual 

tests will not discriminate between many possible physiological 

fluids or fluorescent contaminants. 

Forensic light source is an alternate light source (ALS) that 

may cause semen stains to fluoresce when viewed through an 

appropriate color filter.

• Optimal wavelength is dependent on surface characteristics 

of item.

• Certain surfaces appear to quench the fluorescent reaction.

Argon ion laser causes a similar reaction as ALS.

Long-wave ultraviolet (UV) lamp 

• As a precaution, analysts must wear plastic UV eye 

protection and cover any bare skin that will be exposed, 

such as hands and arms, during the UV examination or 

viewing.

• Semen stains may appear on a dark background. 

• It should be noted that some clothing could fluoresce due 

to such materials as detergents and food stains.

 Figure C-12. A variety of substances, in addition to 

semen, may fluoresce under an ALS. 
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• Avoid prolonged exposure of physiological fluids to the 

lamp.

e. Collection of semen stains: Minimize disturbance, 

transference/swiping and contamination of the stain. Gloves 

should be worn during the collection and handling of the swab. 

Always collect a control sample.

Order of preference for collecting dry semen stains should be: 

1. Collect entire item bearing the stain. Ensure that the stain 

will not flake off or become dislodged. 

2. Cut the stain from carpet, upholstery or other item that 

cannot be collected. 

3. Moisten a sterile swab with distilled water, swab the 

suspected semen stain, and air dry prior to packaging. 

Saliva

a. Label a container that will be used to collect the item. (See 

Bodily Fluids Packaging Guidelines.) 

b. Document the location of the item with photography, 

measurement and sketching, where appropriate. 

c. Cigarette butts and used beverage cans or bottles are common 

types of saliva-containing evidence found at crime scenes. 

d. In sexual assault cases, consider swabbing the breast or other 

bodily areas to collect any potential saliva evidence if case 

circumstances dictate. Use a dry swab or moistened swab 

depending upon the circumstance of the stain.

e. Use gloved hands or forceps to collect the item to prevent 

contamination. Do not lick the envelope flap or cough/sneeze 

on sample, or contamination of the sample may occur. 

Standard/reference sample for Bodily Fluids 

In order to compare DNA types from suspect(s) and victim(s) to 

evidence analysis results, a standard/reference sample must be 

collected. In cases involving semen as evidence, it is not necessary to 

obtain a semen reference standard. Saliva or buccal swab standards 

are recommended.

Note: Adhere to legal standards for search and seizure, if any, for your 

jurisdiction. 

DNA reference material:

Cellular material obtained by buccal swab (see Male Suspect 

Evidence Collection, Including Sexual Assault below) is sufficient 

for DNA analysis.
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 The preferred method is to collect buccal or saliva swabs with 

several clean swabs.

 Finger-pricking the suspect and placing the blood drops on filter 

paper, or specialty paper designed for this purpose, is acceptable.

 This reference material should be handled and processed as 

evidence. 

Male Suspect Evidence Collection, Including Sexual 

Assault 

Equipment Needed 

Paper bags, boxes, and envelopes; cotton-tipped swabs; paper bindles 

or other sterile swab storage container; distilled water; toothpicks; 

clean butcher paper; comb; waterproof pen; evidence tape; protective 

gloves; face protection. Commercial products are also available for 

suspect evidence collection.

 Do not package bloodstained or other biological evidence in 

plastic bags.

 Avoid altering the stain or transferring blood from one portion 

of the garment to another by folding or crumpling the garment. 

 Be careful not to lose or contaminate any remaining trace 

evidence on the garment. 

 Avoid excessive heat when collecting, transporting or storing 

blood and other biological evidence. 

 Avoid unnecessary moisture, water or other liquids. 

 Avoid exposing the bloody evidence to strong light, especially 

UV light.

 Avoid touching, coughing/sneezing over or near the evidence. 

Do not collect any samples without a court order, the suspect’s 

consent or an exigent circumstance.

Documentation 

 Suspects should be photographed in the clothing that they are 

wearing, in both overall and midrange photographs. A separate 

photograph of the face as well as the hands should be taken. 

Any scar, marks, tattoos and injuries, or lack thereof, should be 

photographed close-up. 

 When photographing the subject, include a scale and an 

identification label.
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Marking Evidence 

Evidence labeling: Label a container such as a paper bag or 

envelope with your initials and identification number, the date and 

time, case number, evidence number, location and evidence 

description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence. 

Evidence description: The evidence description should include: 

 type of item (e.g., shirt, pants, shoes) 

 from whom it was obtained 

 color of clothing or other identifying marks on the clothing 

Place suspect in a controlled area away from the victim’s clothing and 

crime scene. Avoid having the collector of the victim’s clothing 

collect the suspect’s clothing so that cross-contamination is 

minimized. 

Trace evidence: Unfold both sheets of paper; lay one on top of the 

other. Examine suspect for any extraneous hairs, fibers, plant 

material, soil, glass, paint, etc., found on the suspect. Place into 

bindle. Fold bindle to contain trace evidence; return bindle to 

envelope; seal envelope and complete label. Note location(s) of 

recovery. 

Have suspect disrobe on the top sheet of paper. Place each garment 

in separate paper bags. Make sure any blood or semen stains are dry 

before packaging. 

Refold top sheet of paper to contain any collected debris. 

Pubic hair combing: Open bindle and place under pubic area. 

Using comb provided, comb pubic region for foreign material; fold 

paper to contain any debris collected and comb. Place in envelope, 

seal envelope and label. 

Pubic hair standard: Pull (using fingers, or witness suspect pulling) 

25 pubic hairs from various areas of the pubic region. Place hairs in 

paper bindle, fold bindle to contain hairs, place bindle in envelope. 

Seal envelope and label. Pubic hair standards are not always required. 

Check with your forensic service laboratory for their preference. 

Penile and digit swabs: Use two cotton-tipped swabs held together 

and dampened with distilled water to swab the exterior of the penis 

and scrotum. Air-dry swabs or use a drying box, place dry swabs in 

envelope, seal and label. For digit swabs (if digital penetration 

indicated): Use 2 cotton-tipped swabs held together and dampened 

with distilled water to swab the fingers of each hand separately, to 
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remove possible vaginal fluid, etc., deposited by the victim. Air-dry 

swabs, place swabs in envelope, seal and label. 

Foreign stains on body: Use two cotton-tipped swabs held together 

and dampened with distilled water to remove possible semen, vaginal 

fluid, saliva, urine, etc., deposited on suspect by victim. 

Bite marks: Photograph as above, swab inside and outside the bite 

mark with a moistened swab, concentrating any material on the tip.. 

Air-dry swabs or use a drying box, place in envelope, seal and label. 

Collect and package evidence from each area separately. Note 

location of recovery. 

Fingernail swabbings/clippings: Swab under nails of each hand or 

clip the fingernails; place resulting residue and swab/clipping in a 

bindle. Fold bindle to contain debris; return bindle to envelope. Seal 

and label. 

Buccal swabs: (Suspect should have rinsed mouth and had nothing 

in mouth for 15 minutes prior to collection of this sample.) Holding 

multiple swabs together, swab the inside of the cheeks. Air-dry swabs 

or place in a drying box, place in envelope, seal and label. 

Head hair standard: Pull (using fingers, or witness suspect pulling) 

25 head hairs from various areas of the head. Place hairs in paper 

bindle. Fold bindle to contain hairs. Place bindle in envelope, seal and 

label. Note: Head hair standards are not always required. Check with 

your forensic service laboratory for their preference. 

Blood standards: See Standard/reference sample for Bodily 

Fluids

Package remaining sheet of paper suspect is standing on. Package 

separately. Close the container and seal the entire opening with 

evidence tape. Write your initials and identification number, and the 

date and time across the evidence tape seal. 

Bombs and Explosives 

Bombs and explosives recognition and collection are best performed 

by specialized personnel. For scene personnel, evidence may be 

observed through smell, sight and sound; these factors should be 

recorded in notes. 

 NEVER attempt to collect any evidence until you have spoken 

with a bombs and explosives investigator or specialist. 

 Follow the instructions provided by the bombs and explosives 

investigator or specialist with whom you speak. 
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Documents 

For crime scene investigations containing voluminous documents, a 

Forensic Document Examiner (FDE) can provide valuable on-site 

support. The FDE can be effective in screening large numbers of 

questioned documents to assist in selecting representative samples 

that are more likely to provide useful information for a specific 

investigation. Similarly, the FDE can effectively screen larger 

numbers of existing known writing samples, e.g., forms within 

records available in personnel offices. The expertise of the FDE for 

these screening tasks can save many hours of investigator work time, 

limit the amount of unnecessary evidence taken for evaluation, and 

consequently reduce the time required for FDE examinations of the 

more relevant documents submitted. 

Equipment Needed 

Paper envelopes1 and boxes; pie boxes lined with sheet cotton2

 Handle documents in a manner that prevents changes or 

alterations of the evidence. Never fold, unfold, staple, attempt to 

reassemble torn paper fragments, or allow shifting of torn or 

shredded paper fragments collected from waste containers, etc. 

Collect all questioned documents found at the scene. Check 

pockets for paper and paper fragments in clothing worn by 

suspects, victims, and other persons of interest at the scene; 

these fragments may be useful in associating the person with 

other document evidence. 

; 

paper towels; rolls or large sheets of window screen fabric; manila or 

other similar folders; clean sheets of paper; waterproof pen; evidence 

tape; protective gloves; face protection.

 Collect all tablets, notepads, spiral-bound notebooks, etc., 

containing visible writing or not, as these may contain pages 

with decipherable indentations and/or paper fragments to 

associate them with questioned documents3

 

1 Toner on documents produced from photocopiers or laser printers, correctible 

typewriting, stamps, glossy photograph surfaces, and other document components 

can adhere to plastic document protectors, resulting in damage when the items are 

removed from the protectors. They should not be used.

. Collect handwriting 

samples: letters, diaries, and other existing written documents at 

2 Pizza and regular pie-shaped boxes containing sheet cotton stapled to both inner 

surfaces will immobilize documents placed within for safekeeping. This is 

particularly critical for charred documents and fragments.

3 Indentations can be present from anonymous letters (some may be yet to surface) 

in an investigation. Paper fragments should be immobilized to avoid shifting and 

mixing that can delay reassembly of torn and shredded documents.
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the scene (standards); also collect requested writing samples 

(exemplars) from victims, witnesses, and suspects who may not 

be available later to render samples of their handwriting. 

 Collect computers, printers, typewriters, adding machines, check 

protectors, rubber stamps, embossers, etc., for comparison 

purposes, when these devices may be related to questioned 

documents in an investigation. Check any service documentation 

for these devices that may also be useful in associating devices 

with questioned documents. 

 Collect paper, printer cartridges (ink, toner, and imaging drums), 

pens, markers, and other supplies that may be related to content 

of questioned documents. 

 Never write on packaging containing a collected document once 

the document is inserted. 

 Avoid altering a stain or mark in any way, other than as 

necessary for evidence collection (e.g., moistening a dried stain 

for swabbing). 

 Always change gloves and use sterile tools when collecting a new 

sample.

 Do not cough, sneeze, or talk over any sample being collected or 

dried, to prevent contaminating the documents with additional 

DNA.

Documentation 

Ensure that the item has been documented as it was found. When 

photographing the item: 

 Include a scale and an identification label.

 When possible, shoot accurately scaled photography. 

 Take one or more location photographs that show the item 

where it was found. 

 Show the relationship of the item to other evidence in the 

photograph. 

Marking Evidence 

When acceptable, mark evidence containers instead of evidence. 

Mark documents for identification as inconspicuously and 

unobtrusively as possible. For instance, make small, limited markings 

in an area not intersecting any of the document printing or writing 

(e.g., very small initials and an abbreviated time/date entry along a 

bottom corner on the back of a document). Use a pencil for 

markings if documents might be examined later for latent prints. 
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Evidence container labeling: Label a container for the object as 

specified by your agency (e.g., your initials and identification number, 

the date and time, evidence number, location, and evidence 

description). When selecting a container:

• For paper that is charred or burned, use a rigid, flat box 

lined with sheet cotton or similar material. 

• Use an envelope when collecting checks, receipts, letters, 

reports, or other similar documents. Always use envelopes 

that are larger than documents collected.

Evidence number: Each piece of evidence must have a unique 

number for identification. This number should correspond to the 

placard next to the evidence when photographing the items at a 

scene.

Evidence description: The evidence description should include: 

• Sufficient detail to distinguish each item from similar items 

collected as evidence.

• Condition of the document. 

• Orientation of the document to prominent nearby 

landmark. 

Label the container just before inserting evidence into them. Seal 

containers as soon as appropriate to avoid cross-contamination, 

unnecessary movement of fragments, etc. 

 Handle documents with gloves to avoid adding fingerprints that 

may obscure latent prints of evidentiary value. 

 Use thicker paper, e.g., pieces of manila folders, to scoop up 

documents and insert them into paper envelopes or paper box 

evidence containers, etc. 

 When necessary, pick up documents by touching the smallest 

area possible, such as one corner. 

 Avoid bending, folding, unfolding, stapling, or otherwise altering 

document evidence.

Drying Wet Documents 

To scoop up wet single-page documents, use cardboard sheets, e.g., 

pieces of manila folders. Wet documents can be dried by placing 

them on a clean piece of paper towel or a sheet of window screen, 

and then placed in a secure location for drying. Sheets of clean paper 

towels, etc., should be spread beneath the area used to handle moist 

documents in order to collect any trace evidence that falls from the 
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documents during handling. The items used to catch trace items may 

also need to be collected and handled as evidence.

 DO NOT attempt to unfold wet documents, as this should 

ONLY be done by laboratory forensic document examiners. 

 Documents in a container of water or other liquid may need to 

be kept in the liquid for transportation to the laboratory and 

processing by forensic document examiners. 

 Depending on the nature of the investigation, it may be 

important to avoid potential contamination that results from 

handling two documents in the same area at the same time.

Packaging

a. Place dried documents between clean sheets of paper, such as 

paper towels or cardboard sheets, to provide a protective 

covering before placing them into labeled evidence containers. 

Label evidence containers before placing objects into them to 

avoid degrading existing evidence. Handle documents carefully 

to avoid bending, folding, or otherwise degrading them. Handle 

documents appropriately to protect any latent prints that may 

exist. 

b. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time, as required, across the evidence tape seal.

c. Carefully pick up and fold the paper used as a catch-surface 

beneath drying documents. Retain any trace evidence that may 

have fallen onto this catch-surface paper from the evidence, by 

folding it inward from the corners. Then place the catch-surface 

paper in a labeled evidence container, as needed. 

d. Label the folded paper, indicating the evidence number of the 

item that was dried; e.g., “This paper was used below evidence 

#36 while it was drying.” 

e. Repackage the object using the original packaging and container 

if possible. Save all original packaging with evidence if it is not 

used for repackaging. 

f. If the original container cannot be reused: 

• Label the container indicating the evidence number of the 

item; e.g., “Original packaging for evidence #36.”

• Put the labeled, original packaging into a new container 

with the evidence it was used to collect.
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Firearms 

Packaging Guidelines 

Equipment Needed 

Rigid, paper firearm and ammunition boxes, or other rigid, paper 

containers; clean paper; waterproof pen; evidence tape; evidence tags; 

and protective gloves. 

 Avoid altering any stain on the firearm.

 Firearms should be unloaded and placed in a safe condition at 

the point of collection. If the collector is unsure of the proper 

procedure, assistance should be sought from a competent source 

such as a firearms instructor, departmental armorer, or an 

on-site firearm examiner. Always follow your department 

regulations. 

 Ship firearms and ammunition according to your Department 

and the U.S. Department of Transportation’s regulations. 

 Do not cough, sneeze, or talk over any sample being collected or 

dried. 

Documentation

a. When photographing the firearm, include a scale and an 

identification label.

b. Take one or more location photographs that depict the object 

where it was found in relationship to other evidence at the 

scene.

c. Turn over the firearm, and photograph the other side if stains, 

serial number, or safety position is apparent on that side. 

d. If no serial number is present, mark the firearm with identifying 

data, such as your initials and the time and date on at least one 

component that cannot be removed. Also, place identifying data 
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on removable component(s), such as cylinder, grips or 

ammunition magazine.

e. When there is no serial number on the firearm due to the 

firearm’s age, or the removal or defacement of the number, 

mark the firearm on non-removable parts, such as the barrel and 

the frame.

f. Photograph the firearm to capture any existing stains (such as 

blowback), the serial number, and the safety position. 

g. Place the mark in a location that will not interfere with existing 

markings.

Marking Evidence 

Evidence labeling: Label the firearm box or a container for the 

object with your identifying data, such as initials and identification 

number, the date and time, evidence number, location, and evidence 

description. 

Evidence number: Each piece of evidence must have a unique 

number or identifying data. This number should correspond to the 

placard next to the evidence.

Evidence description: The evidence description should include: 

• Type of firearm

• Location of any stains on the firearm

• Whether the stains are wet or dry

• Location of the firearm

Label the container just before collecting an object, and seal the 

container immediately after collection. These actions help to protect 

the chain of custody. 

When handling the firearm, do not touch areas of the firearm where 

latent fingerprints are likely to be found (such as on smooth 

surfaces). Handle the firearm by touching only the areas that are 

checkered or knurled. 

Specific Items Procedure: Revolvers 

A revolver is defined as a repeating firearm that has a cylinder 

containing multiple chambers and at least one barrel for firing. 

a. Mark the cylinder with your initials and the time and date. Put 

the mark in a location that will not interfere with existing 

markings.

b. Mark the cylinder position. 

c. Document the condition of the firearm. Information needed 

includes: 
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• Number of rounds in the chamber 

• Type and location of any stains 

• Serial number, make, model, and caliber

d. Protect the barrel, chamber, or other operating surfaces from

contact with other objects. 

e. Handle the firearm by touching only those areas that are unlikely 

to contain latent fingerprints, such as areas that are checkered or 

knurled.

Note: Check agency evidence submission policy. For firearms 

that will be analyzed by a trained firearms examiner, a particular 

agency may require or prefer alternative procedures for 

submission of evidence to their laboratory. 

f. Determine which way the cylinder turns. Cylinder turn can be 

either clockwise or counterclockwise, from the perspective of 

the shooter. 

g. Create a cylinder diagram like the one below:

 

Figure C-13. Cylinder diagram with assigned numbers for each 

chamber 

On the diagram, assign a number to each chamber in the 

cylinder. The chamber below the hammer/firing pin is number 

one. From number 1, continue numbering chambers as you 

move around it in the direction that the chamber turns. The 

diagram should show the state of each chamber – whether it 

contains a live or spent cartridge case, or is empty.

h. Document in your notes the type of projectiles/bullets 

contained in each chamber of the cylinder that you observe. It is 

important to note the type of projectile/bullet used because 

more than one type could be in use in the firearm at the same 
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time; e.g., “Chamber one (1) contained .357 caliber ‘Acme’ full 

metal jacket.”

Firearms should be unloaded and placed in a safe 

condition at the point of collection. If the collector is 

unsure of the proper procedure, assistance should be 

sought from a competent source such as a firearms 

instructor, departmental armorer, or an on-site firearm 

examiner. 

i. Remove the cartridge or case from the chamber carefully to 

avoid disturbing any potential trace evidence or latent prints on 

it. Mark each cartridge or case with the related chamber number.

j. When labeling the cartridge or case, make all marks in or near 

the mouth of the casing. Do not label a cartridge or case near 

the rim, head, or primer. 

k. Mark each cartridge or case with the number of the chamber in 

which they were contained. The cartridge or case directly below 

the firing pin is in position number one. Start with number one 

and move in the direction of the cylinder rotation. Label the 

container into which you will place the ammunition with the 

same chamber number as you wrote on the cartridge or case.

If necessary, wrap each object carefully so as to protect any 

potential trace evidence. 

l. Mark each chamber once you have marked the ammunition. On 

each side of the strap, mark the chamber with the number it was 

assigned when numbering cartridges and cases.

m. Place the firearm, with available filled ammunition container(s), 

in the rigid box or container that you prepared for the firearm. 

n. If you are collecting multiple firearms, be sure to package each 

firearm and associated ammunition separately from other 

firearms.

o. When the firearm is too large to fit into a container, securely 

attach an evidence tag to it. The evidence tag should include the 

following firearm information: 

• Caliber

• Make

• Model 

• Serial number 

p. Place the diagram into the labeled container with the firearm and 

filled ammunition containers. Make a copy of the diagram for 

your notes before placing the original copy into the box. 
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q. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

r. Consider the possible presence of bodily fluids, latent prints and 

trace evidence when handling the firearm or submitting it for 

processing. 

Specific Items Procedure: Other Firearms (e.g., SemiAutomatics, Automatics, Rifles) 

Refer to firearms packaging guidelines

a. Document the identity of the firearm or the listed firearm’s 

markings. 

b. If no serial number exists, mark the firearm with your initials 

and the time and date on at least one part that cannot be 

removed. 

c. When the firearm has a bolt, mark the bolt with your initials and 

the time and date.

d. Information needed includes: 

• The safety position – on or off 

• Existence of ammunition in the chamber 

• Type and location of any stains 

• Serial number, make, model, and caliber or gauge 

e. Protect the barrel, slide, chamber, and other operating surfaces 

from contact with other objects. 

f. Handle the firearm by touching only those areas that are unlikely 

to contain latent fingerprints, such as areas that are checkered or 

knurled. 

Firearms should be unloaded and placed in a safe condition at 

the point of collection. If the collector is unsure of the proper 

procedure, assistance should be sought from a competent 

source such as a firearms instructor, departmental armorer, or 

an on-site firearm examiner. 

a. If removing ammunition, mark each cartridge, case, shell, or 

magazine as it is removed from the firearm.

b. Some departmental policies allow labels to be placed directly on 

a cartridge or case. When labeling the ammunition, make all 

marks near the mouth of the casing. Do not label ammunition 

near the rim, head, or primer. 
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c. Mark each cartridge or case with the number of the chamber or 

position in the magazine in which they were contained, starting 

with number one, or use another schema that is consistent 

within your department for the way that the ammunition is 

stored in the firearm.

d. Document, in your notes, the type of projectile/bullet that you 

observe in the chamber. 

e. It is important to note the type of projectile/bullet used; e.g., 

“Chamber contained .357 caliber ‘Acme’ full metal jacket.” 

f. Mark each magazine to note where cartridges, cases, or shells are 

found in it. 

g. On each side of the magazine, mark the position in which each 

numbered cartridge or shell is found. 

h. The mark should include the number assigned to the related 

cartridge, case, or shell. The number reflects the position in the 

magazine in which cartridge, case, or shell is found.

i. Label a container into which you will place the ammunition with 

the same number as you wrote on the cartridge, case, shell, or 

magazine. The label on the box or container should include the 

number of the position in the magazine in which cartridge or 

case was found.

j. If necessary, wrap each object carefully so as to protect any trace 

evidence on it.

k. When the firearm does not fit into a container, attach an 

evidence tag that describes the caliber, make, model, and serial 

number. 

l. The trigger guard is frequently the attachment point for the 

evidence tag.

m. The evidence tag should include the following information: 

• Caliber

• Make

• Model 

• Serial number 

Label cartridge casing 

in this area
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n. Place the firearm and ammunition container(s) in the container 

that you prepared for the firearm. 

o. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

p. Consider the possible presence of bodily fluids, latent prints and 

other trace evidence when handling the firearm or submitting it 

for processing. 

Ammunition 

Specific Items Procedure: Embedded Ammunition 

Equipment Needed 

Hand tools (hammer, chisel/screwdriver, metal and plastic forceps, 

hand saw, etc.); flashlight; paper bags, boxes, or envelopes; clean 

paper; waterproof pen; evidence tape; personal protective equipment. 

 Gunshot residue and fingerprints are extremely fragile. Collect 

gunshot residue and fingerprints as soon as possible. 

 When possible, collect the entire object in which the 

ammunition is embedded. 

 Always wear protective gear when handling objects that could 

cut or otherwise cause injury to you. 

 Do not cough, sneeze, or talk over any sample being collected or 

dried. 

Documentation

When photographing the object: 

• Include a scale and an identification label.

• Take one or more location photographs that show the 

object where it was found. 

• Show the relationship of the object to other evidence in the 

photograph. 

• Use rods or strings to show ammunition entry and exit 

points 

a. In your notes, document characteristics of the object in which 

the ammunition is embedded. Information needed includes: 

• Type of object 

• Condition of object 

• Location of object 
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• Color of object 

• Location of ammunition in the object 

• Type of ammunition, when possible 

• Apparent entry angle 

b. Collect the object containing the embedded ammunition, when 

possible. 

c. If you were unable to collect the entire object, collect the 

embedded ammunition: 

1. Use available tools (hammer, chisel/screwdriver, forceps, 

hand saw, etc.) to carefully loosen and remove the 

ammunition. 

2. Handle the ammunition carefully to avoid destroying marks 

on it. Pad the tips of the forceps, for example, to protect 

the extracted item from forceps marks.

3. When fragments and other items related to the embedded 

ammunition are found in the object or fall from the object, 

collect them also.

d. During collection, handle the object very carefully to avoid 

damaging evidence, or dislodging the embedded ammunition or 

any related residue. 

Marking Evidence 

Evidence labeling: Label a container for the object with your initials 

and identification number, the date and time, evidence number, 

location and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of object 

• Color of object 

• Type of ammunition, when possible 

• Location of the ammunition in the object, including 

orientation to key object feature(s); e.g., “Bullet of 

unknown type in piece of unstained wood beam in garage 

next to washing machine.”

Label the container just before collecting an object, and seal the 

container immediately after collection. 
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Packaging

Place the object in the labeled container. Seal the opening of the 

container with evidence tape. Write your initials and identification 

number, and the time and date across the evidence tape seal.

Specific Items Procedure: Fired Cases or Wads 

Equipment Needed 

Hand tools (hammer, chisel/screwdriver, forceps, hand saw, etc.); 

flashlight; paper bags, boxes, envelopes, and bindles; clean paper; 

waterproof pen; evidence tape; personal protective equipment. 

 Gunshot residue and fingerprints are extremely fragile. Collect 

gunshot residue and fingerprints as soon as possible. 

 Use a “druggist fold” to create bindles that will be used to 

hold cases and wads. 

 Always use clean gloves when handling evidence. 

 Do not cough, sneeze, or talk over any sample being collected or 

dried. 

Documentation

When photographing the object: 

• Include a scale and an identification label.

• Take one or more location photographs that show the 

object where it was found. 

• Show the relationship of the object to other evidence in the 

photograph 

Marking Evidence 

Evidence labeling: Label a container for the object with identifying 

data, such as your initials and identification number, the date and 

time, evidence number, location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of case or wad

• Type of weapon used, when known 

• Location where the case or wad was found; e.g., “.357 

caliber spent case found lying on the garage floor, beneath 

the stool.” 
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 Label the container just before collecting an object, and seal the 

container immediately after collection. 

 Pick up the case or wad carefully to avoid damaging or 

dislodging fingerprints or other evidence. Use tools available to 

carefully loosen and remove the case or wad when it is difficult 

to reach and to avoid damaging evidence.

Packaging

a. Place the case or wad into the labeled envelope.

b. When multiple cases or wads are collected from the same area, 

place each case or wad in a separate container. Each container 

must be individually labeled. 

c. Individual, labeled containers with cases found in the same area 

can be packaged together for transport. Wads should not be 

packaged with other ammunition or related objects. 

d. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

Specific Items Procedure: Loose, Unspent Ammunition 

Equipment Needed 

Hand tools; flashlight; paper bag, box, or envelope; waterproof pen; 

evidence tape; protective gloves; face protection. 

 Gunshot residue and fingerprints are extremely fragile. Collect 

gunshot residue and fingerprints as soon as possible. 

 Always use clean gloves when handling evidence.

 Do not cough, sneeze, or talk over any sample being collected or 

dried. 

Documentation

Photograph the ammunition and the location where it was found. 

When photographing the object: 

• Include a scale and an identification label.

• Take one or more location photographs that show the 

object where it was found. Show the relationship of the 

object to other evidence in the photograph. 

Marking Evidence 

Evidence labeling: Label an envelope for the ammunition with your 

initials and identification number, the date and time, evidence 

number, location and evidence description. 
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Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of ammunition 

• Location of unspent ammunition 

• Orientation of ammunition relative to firearm or other 

point of interest, when possible; e.g., “Acme full metal 

jacket case for a .357 caliber revolver found on garage 

floor.”

Label the envelope just before collecting an object, and seal the 

envelope immediately after collection. 

Photograph the ammunition and the location where it was found. 

When photographing the object:

• Include a scale and an identification label.

• Take one or more location photographs to depict the object 

where it was found in relationship to other evidence in the 

photograph. 

Pick up the ammunition carefully to avoid dislodging fingerprints or 

other evidence. Place the ammunition into the labeled container.

Unless departmental regulations state otherwise, do not wrap the 

casing. Wrapping it could disturb evidence that has not already been 

collected.

Packaging

a. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

b. Collect and package any additional ammunition or related items. 

c. When multiple pieces of ammunition or related objects are 

found in an area, place each object in a separate container. Each 

container must be individually labeled. Those individual 

containers can then be packaged together for transport. 

Tool Mark Evidence 

A tool mark is any impression, scratch, gouge, cut, or abrasion made 

when a tool is brought into contact with an item, leaving an 

impression of the tool. In some cases, tool mark identification may 

link a person to the tool used in the commission of a crime. 
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Equipment Needed for Casting

Casting kit (e.g., Duplicast©, Mikrosil©, silicone-type sealant); mixing 

pad; stirring stick; flashlight and other available light sources; paper 

envelopes and boxes; waterproof pen; measuring tape/ruler; 

identification labels; protective gloves; face protection.

 Photograph the impression before casting it. 

 When making a cast, be prepared to act quickly and 

methodically. Time is often a critical factor in successfully 

making a cast.

 Always use clean gloves when handling evidence.

Documentation

Photograph the impression. When photographing the object: 

• Include a scale and an identification label.

• Take at least one photograph where the camera lens is 

perpendicular or “orthogonal” to the tool mark surface. 

• Take one or more mid-range location photographs that 

depict the object where it was found. 

• Show the relationship of the object to other evidence in the 

photograph. 

Evidence Marking 

Evidence labeling: Label a container for the object with identifying 

data, such as your initials and identification number, the date and 

time, evidence number, location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of item being cast

• Location of the item being cast

• Orientation of the item being cast; e.g. to the north, to a 

feature of the object with the impression on it, or to a 

nearby object.

Casting the Evidence

a. Label the container just before collecting an object, and seal the 

container immediately after collection. 

b. Clean out loose material from the impression, when possible, 

without disturbing the impression. Never discard a cast, 
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regardless of condition when removed from the impression. Be 

sure to save and submit all casts to the laboratory. 

Packaging

a. Close the container and seal the entire opening with evidence 

tape. Write your initials, identification number, and the date and 

time across the evidence tape seal.

b. Make sure that the container is also labeled with a description of 

the item cast, appropriate identifying data, such as your initials 

and identification number, the date and time, location and, when 

possible, the evidence number. 

c. If the item with the tool mark is collected, it should be packaged 

separately from a suspect tool(s) to prevent any additional 

marks, impressions or other damage. Never place the 

suspected tool(s) into the impression. 

Trace Evidence 

Trace Evidence deals with the collection of all forms of matter, 

natural or manufactured, usually very tiny materials, but may also be 

larger forms of matter. Examples are gas from a container (bag or 

metal cylinder), hair, pollen, stains (non-biological), volatile liquids, 

fibers, paint, glass and soil. Trace evidence can be easily destroyed, 

contaminated, or transferred, so take precautions when approaching 

the scene.

 Since trace evidence items are often difficult to see without the 

aid of magnification, the prudent course of action is to collect 

items of evidence that may contain trace evidence. 

 Care should be used when collecting weapons, as improper 

handling and packaging can compromise the trace evidence. 

 Careful photography, documentation, and sketching are critical 

for the optimal use and interpretation/reconstruction of trace 

evidence.

 Always package items to prevent damage or alteration of the 

evidence: use packaging that corresponds to the size and type of 

the item and evidence.

 Always collect sufficient amounts of the trace evidence when 

possible. 

 Do not package evidence if it is wet or damp.

 Do not package exemplar, standard/reference, or control 

samples with evidence. Package separately.

 Use of a bright or alternative light source may help in locating 

trace evidence.
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 Standard, reference, and control samples should be collected for 

laboratory comparison, examination, or elimination purposes. 

Collect enough for multiple analyses. 

 Always wear powder-free gloves while collecting trace evidence 

to avoid contamination. 

 Change gloves as often as needed to minimize contamination. 

Trace Evidence Documentation and Packaging Guidelines 

Equipment Needed 

Paper/manila envelopes; paper bags; 4-inch and/or 6-inch glassine 

weigh paper (for paper bindles or self-made envelopes); 2.5- to 4-inch 

wide clear adhesive tape, clear acetate sheet protectors or clear 

secondary liners (non-silicone or silicone coated one-side); 

tweezers/forceps with different tips; one-quart and one-gallon metal 

friction lid cans with fitting lids (clean, empty paint cans); glass jars, 

bottles, and vials with Teflon®-lined screw cap lids or other 

appropriate air-tight inert lid; glass Pasteur pipettes with rubber bulb 

or all-in-one plastic transfer pipettes; transparent tape; cotton-tipped 

swabs; swab-drying boxes, or other sterile swab storage containers; 

distilled water; cutting tool (knife, scalpel, single-edge razor blade, 

drywall saw, carpet knife, or box cutter); waterproof pen; evidence 

tape; powder-free protective gloves; face protection.

 Do not package evidence stained with bodily fluids or other 

liquids in plastic bags. 

 Avoid altering the trace evidence in any way, other than is 

necessary for evidence collection (e.g., scraping paint from a 

metal surface). 

 When possible, when storing evidence, use a freezer/refrigerator 

that is dedicated to evidence storage to guard against potential 

biohazard contamination or explosion hazards. 

 Do not cough, sneeze, talk, or scratch yourself over any sample 

being collected or to be collected. 

 When using tweezers/forceps, use only sufficient pinching force 

to collect item to prevent altering or damaging the trace 

evidence.

 Keep adhesive tape to be used in lifting evidence in a plastic bag 

until just before use to prevent contamination of the edges/side 

of the tape roll. Put unused portion of tape roll back into the 

plastic bag when finished with tape lifting. 

 Do not place trace evidence directly into manila envelopes (of 

any size), paper envelopes, or paper bags without placing the 
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evidence into a smaller, leak-proof container such as a glassine 

bindle, canister, Teflon-lined screw cap glass vial (or bottle/jar 

as size dictates), or other container. 

 Keep clear acetate sheet protectors free from contamination by 

storing in an appropriate size manila envelope or plastic 

resealable bag.

 Careful photography, documentation, and sketching are critical 

for the optimal use and interpretation/reconstruction of the 

evidence.

 To reduce breakage and loss of evidence, secure glass containers 

(e.g., vials, bottles, jars) in cushioned metal friction lid cans with 

lids. Cushioning material may be balled-up paper towels. 

 Using a swab to collect a trace evidence sample – whether liquid, 

powder, gel, or other form of matter, evidential or control – is a 

last resort.

Documentation

Ensure that the portion of the object with the stain or other trace 

evidence has been photographed and documented in notes and 

sketches. When photographing the object: 

• Include a scale and an identification label.

• Photograph with and without scale. 

• Take one or more location photographs that show where 

the object was found in relation to other objects in the 

crime scene.

• Show the relationship of the object to other evidence in the 

photograph. 

Marking Evidence 

Evidence labeling: Label a container for the evidence object with 

your initials and identification number, the date and time, evidence 

number, location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard or 

identification label next to the evidence and the evidence log as 

appropriate. 

Evidence description: The evidence description includes: 

• Type of evidence (paint, plant stem, glass, etc.)

• Original location of the trace-evidence-containing object 

• Location of the trace evidence on the object (as 

appropriate) 
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When describing non-DNA-related stains, use the word “apparent” 

or the phrase “of unknown origin” when the source of the stain is 

not scientifically identified; e.g., “Pillow containing a brown stain of 

unknown origin found on the lower bed sheet on the bed.” 

Label the container just before collecting the object, and seal the 

container immediately after collection. These actions help to protect 

the integrity of the trace evidence and the chain of custody. 

Packaging Wet Evidence 

a. If the item is wet, determine if the liquid is significant or relevant 

to the case (for instance, acid splashed on a victim). You may 

not wish to dry the item if the liquid is relevant to the case. 

b. If the liquid is not relevant, place item on a clean piece of paper 

in a secure location used for evidence drying, such as a drying 

rack, until it is dry.

c. If you have access to a drying rack, dry the item in it. Place a 

clean piece of paper below the evidence to be dried in the drying 

rack. Lay the item on or hang it above the paper.

d. If you do not have a drying rack: 

• Lay a clean piece of paper on a clean flat surface (e.g., table 

top) in a secure location where the item will not be 

disturbed and contamination or loss of the trace evidence 

due to drafts created by people walking by will be 

minimized.

• Carefully place the item on or hang it over the paper. If 

possible, gently fold the paper over the object to cover it 

from airborne particles (such as hair or fibers) that might 

land on it. 

• Allow the item to dry naturally. Never expose it to heat or 

significant drafts, such as from a blow dryer. Avoid 

exposing the sample to direct sunlight. 

e. Do not place two items in the same container for drying 

purposes. 

f. If the liquid is relevant to the case, determine if the liquid is: 

• related to a fire or explosion and in or on an object (e.g., 

ignitable liquid in carpet) – contact fire investigator or 

bomb technician or forensic fire debris or explosives 

analyst as appropriate

• involved in injury or damage to another person or property 

and on an object 
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o if small sample – secure in appropriate size glass 

container with a Teflon-lined screw cap lid or other 

airtight, non-reactive lid.

o if large sample – document location of wet area by 

photography, notes, and sketches, then cut or remove 

the wet area and treat as if small sample

• soaking the object or there are drops of liquid on the 

surface (non-porous surface) – using gloved hands, attempt 

to squeeze liquid drops into glass container or use swab to 

collect drops and treat as small sample

• forming a large drop or pool of liquid. Transfer liquid using 

glass Pasteur pipette or plastic transfer pipette into 

appropriate size glass vial. 

g. Consider appropriate hazardous labeling. 

Packaging Dry Evidence

a. When object has dried, carefully pick up and fold the paper on 

or over the object that has dried. Contain any trace evidence that 

may have fallen on the paper. 

b. Label the folded paper, indicating the evidence number of the 

item that was dried; e.g., “This paper was used below evidence 

#36 while it was drying.”

c. If possible, repackage the object using the original paper and 

container. 

d. If the original container cannot be reused: 

1. Save all original packaging as evidence if it is not used for 

repackaging.

2. Put the labeled, original packaging into a new container 

with the evidence it was used to collect.

3. Label the container indicating the evidence number of the 

item; e.g., “Original packaging for evidence #36.”

e. When possible, place glass vials, bottles, and jars into a 

cushioned metal friction lid container to reduce breakage. 

f. To reduce breakage and loss of evidence, secure glass containers 

(e.g., vials, bottles, jars) in cushioned metal friction lid cans with 

lids. Cushioning material may be balled-up paper towels. 

g. Do not place trace evidence directly into manila envelopes (of 

any size), paper envelopes, or paper bags without placing the 

evidence into a smaller, leak-proof container such as a 
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glassine/paper bindle, canister, Teflon-lined screw cap glass vial 

(or bottle/jar as size dictates), or other container. 

h. Keep clear acetate sheet protectors free from contamination by 

storing in an appropriate size manila envelope or plastic 

resealable bag.

i. Tape lifts should be placed onto clean, clear (not translucent) 

acetate sheet protectors. Then place the sheet protectors into 

appropriate size manila envelope.

Collecting a Control or Comparison Sample

A control sample is one that the collector knows, that does not 

appear to have evidence present. It represents the matrix material on 

which the evidence rests, for instance a piece of wallboard or 

carpeting. A comparison sample may have evidence present 

without the collector’s knowledge (e.g., fire debris), but represents 

the matrix material on which the evidence rests or is made of the 

same matrix material as the evidence (e.g., glass fragments collected 

from a broken window). It will be compared to the evidential sample. 

These will be referred to as “control” samples. Label a second 

container for the control sample with your initials and identification 

number, the date and time, evidence number, location of the control 

in relation to the original sample, and a description of the control 

sample. Clearly identify this sample as a control (or comparison) 

sample (e.g., write the words “Control Sample” or “Comparison 

Sample” in bold print on the container). 

Control sample number: Each piece of evidence, including the 

control sample, must have a unique number. A letter or number may 

be appended to the original evidence number to denote the control 

sample (e.g., if the original evidence number was #32, the control 

sample could be #32A or #32.1.)

Control sample description: The description includes: 

• Type of sample or matrix material (e.g., wallboard)

• Location of the material

• Location of the sample in relation to the evidence sample 

Collect a control sample: Using the appropriate tool, cut out, collect, 

or remove a portion of the same matrix material on which the 

evidence sample rests or was part of the evidence sample (e.g., paint 

smear evidence on paint). Locate an area of the same material from 

which the original trace evidence sample was taken, but without 

evidence being present (e.g., undamaged area of paint). 

Cut out, collect, or scrape the control or comparison sample using, as 

appropriate, a scalpel, utility knife, wallboard saw, carpet knife, single 

edge razor blade, or scissors. (Whenever possible, use a clean blade; 
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never use a blade that was used to cut an evidential or other control 

sample without changing or thoroughly cleaning.) If multiple layers 

(such as carpet and carpet pad or multi-layer paint) of material were 

observed or collected in the original sample, collect all of the multiple 

layers for the control or comparison sample. 

A liquid sample should be collected using a glass or plastic transfer 

pipette and placed into a glass Teflon-lined screw-cap vial or bottle of 

the smallest permitted size for the sample. Using a swab to collect a 

liquid trace evidence sample (evidential or control) is a last resort. Put 

the swab into an appropriate size screw-cap vial or other air-tight 

container. 

Trace Evidence Collection Procedures 

Remove an Entire Portable Object 

Part of a Non-Portable Object 

Scrape from Non-Portable Object 

Lift with Tape or Adhesive 

Recovery with Tweezers 

Swab a Surface 

Soil or Rock Samples 

Gunshot Residue 

Collection Procedure: Remove an Entire Portable Object 

Follow Trace Evidence Documentation and Packaging 

Guidelines

1. Photograph and document the object with the apparent trace 

evidence.

2. Whenever appropriate, wrap the entire object in clean (butcher) 

paper or in a brown paper bag. 

 Only wrap an object when doing so will not disturb the 

position of a stain or other trace evidence (glass fragment, 

metal fragment, paint smear, etc.).

 Objects should be wrapped in clean paper when the location 

or pattern of the stain or other trace evidence is significant 

(such as a spatter pattern) or the object is saturated and 

liquid will leak through the container if not wrapped. 

3. Position the paper to keep the trace evidence intact in its original 

form. Avoid transferring any of the trace evidence to another 

portion of the object. 
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4. Place the wrapped object into the brown paper bag or other 

labeled container.

5. If an object is too large to be packaged in a container, protect 

the stain or trace evidence area(s) with clean paper during 

transport. 

6. Close the container and seal the entire opening with evidence 

tape. Write your initials, the identification number, and the date 

and time across the evidence tape seal. Ensure that any small 

openings in the package are also sealed. Place initials over these 

seals.

7. If an object is too large to be packaged in a container, protect 

the relevant area(s) with clean paper during transport.

8. Repackage the object using the original packaging, if possible, 

and reseal. 

9. Store the object in the sealed container. 

10. Place the container in a secure, dry storage area.

11. Never expose the container to extreme heat, such as from a 

heater vent.

12. Avoid exposing the container to direct sunlight. 

Procedure: Cut from a Non-Portable Object 

Follow Trace Evidence Documentation and Packaging 

Guidelines

a. Label a container for the object to be collected with your initials 

and identification number, the date and time, evidence number, 

location, and evidence description. 

b. Photograph, sketch, and take notes on the object with the trace 

evidence or stain. 

c. Collect a larger area than where the trace evidence is observed, 

especially if the shape or pattern of the trace evidence or stain is 

significant (e.g., paint spray, broken glass pane, etc.).

d. If possible, cut out the entire area using a scalpel, single-edge 

razor blade, utility knife, carpet knife, dry wall saw, scissors or 

other tool as needed to remove section. If the trace evidence has 

been absorbed into multiple layers (such as carpet and carpet 

pad), collect a cut-out from each layer.

e. If the entire stained or evidence area is too large to collect as one 

piece, using the appropriate tool, cut out a smaller section of the 

area and label to re-assemble the sections later if needed.
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f. On the side of the cut-out opposite the side with a stain or trace 

evidence, mark the orientation of the cut-out to north when 

collected. Be careful not to dislodge trace evidence while 

marking.

g. If the item is “wet”, determine if the liquid is water, a biological 

fluid, volatile, or hydrocarbon. 

1. If water or biological fluid, place it on or over a clean piece 

of paper and allow it to dry before packaging. See 

Packaging Wet Evidence. 

2. If the liquid is volatile, acidic, caustic, or a hydrocarbon, the 

liquid itself may be significant evidence and must be 

packaged in an airtight container to prevent evaporation. 

Contact a fire investigator, fire debris analyst, or bomb 

technician for instructions. (See Trace Evidence 

Documentation and Packaging guidelines.) 

h. Whenever appropriate, wrap the cut object in clean paper, 

glassine bindle, or place in appropriate size glass or plastic 

container or metal friction lid can. 

i. Close and seal the labeled container after having placed the 

object into it. Write your initials and identification number and 

the date and time across the evidence tape seal.

j. If an object is too large to be packaged in a container, protect 

the area(s) with clean paper during transport.

Control sample: Label a second container for the control sample 

with your initials, identification number, the date and time, evidence 

number, location of the control in relation to the original sample, and 

a description of the control sample. Clearly identify this sample as a 

control or comparison sample (e.g., write the words “Control 

Sample” or “Comparison Sample” in bold print on the container). 

Control sample number: Each piece of evidence, including the 

control sample, must have a unique number. A letter or number may 

be appended to the original evidence number to denote the 

comparison or control sample (e.g., if the original evidence number 

was #32, the control sample could be #32A or #32.1.)

Control sample description: The description includes: 

• Type of sample or matrix material (e.g., wallboard)

• Location of the material

• Location of the control sample in relation to the evidence 

sample

Collect a control sample: Using the appropriate tool, cut out or 

remove a portion of the same matrix material on which the evidence 
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sample rested or was part of the evidence sample (e.g., locate an area 

of the same material from which the original trace evidence sample 

was taken but without evidence being present such as an undamaged 

area of paint). 

a. Cut out the control or comparison sample using, as appropriate, 

a scalpel, utility knife, wallboard saw, carpet knife, single-edge 

razor blade, or scissors. (Use a clean blade; never use a blade that 

was used to cut an evidential sample on a control sample 

without a thorough cleaning or replacement.) If multiple layers 

of material (such as carpet and carpet pad or multi-layer paint) 

were observed or collected in the evidential sample, collect all of 

the multiple layers present for the control or comparison 

sample.

b. On the opposite side of the evidential side of the original noncontrol sample, mark the orientation of the cut-out to north 

when collected.

c. Whenever appropriate, wrap the control sample in clean paper 

or other appropriate containment. 

d. Place the object into the labeled container. If an object is too 

large to be packaged in a container, protect the sample with 

clean paper during transport. 

e. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

Treat the control sample with the same care as you treat the 

transfer evidence sample.

Collection Procedure: Scrape from Non-Portable Object 

Follow Trace Evidence Documentation and Packaging 

Guidelines

Equipment Needed 

Paper/manila envelopes; brown paper bags; 4-inch and/or 6-inch 

glassine weigh paper (for paper bindles or self-made envelopes); 

tweezers/forceps with different tips; cutting tool (knife, scalpel, 

single-edge razor blade, or box cutter); waterproof pen; evidence 

tape; powder-free protective gloves; face protection.

 Use scraping when the entire object may not be collectable (e.g., 

quarter panel of car, etc.)

 Often scraping is used to collect evidence on non-porous 

surfaces too large to collect, but may be used to collect evidence 

on porous surfaces (e.g., stain on car seat, furniture fabric, 

canvas, etc.)
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 Paint transfer is commonly located on the object on the 

impacted surface.

 Collect the clothing and shoes of people who have been in the 

area of the painted surface at the time of and since the impact.

 Collect paint control-comparison samples, when the entire item 

cannot be removed, using the scrape method. 

 Always use clean and/or fresh, disposable scraping tools. 

Change blades frequently when applicable. 

 Always change gloves and use thoroughly cleaned tools when 

collecting each new sample (between every evidential or control 

sample.)

 The stain or marking should be dry for scraping. 

 Do not use a commercially manufactured envelope of any kind 

as they have gaps that permit leakage. If a commercial envelope 

must be used, seal around all edges of the envelope with tape. 

 Label a container for the object to be collected with your initials 

and identification number, the date and time, evidence number, 

location, and evidence description. 

 Photograph, sketch, and take notes on the object with the trace 

evidence or stain. 

 Collect as much of the evidence area as possible using a scalpel, 

single-edge razor blade, utility knife, or other tool as needed to 

remove section. 

 On vertical surfaces, place or tape a self-made envelope or 

glassine bindle below the area to be scraped before scraping to 

capture all of the scraping. 

 By scraping the sample, typically all shape or pattern of the trace 

evidence or stain is lost. 

Evidence labeling: Label a container for the evidence-scraped 

object with your initials and identification number, the date and time, 

evidence number, location, and evidence description. Use a druggist 

fold to create a glassine paper bindle or self-made envelope.

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence and the evidence log as appropriate. 

Evidence description: The evidence description should include the: 

• Type of scrape evidence (paint, apparent vomit, etc.)

• Original location of the object containing the trace evidence 
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• Location of the trace evidence on the object (as 

appropriate) 

a. Label an appropriate container just before collecting the 

scraping, and seal the container immediately after collection.

b. Place a large sheet of clean paper beneath the area that you will 

scrape. The paper is used to contain any debris that breaks loose 

while you are scraping. Avoid standing on the paper while you 

scrape.

c. Place or tape a self-made envelope or glassine bindle below the 

area to be scraped before scraping to capture all of the scraping 

possible. Do not use a commercially manufactured envelope of 

any kind as they have gaps that risk leakage.

d. Scrape off as much of the material as possible and go as deep as 

possible to obtain all layers. 

Note: When scraping flakes, attempt to leave the flake as intact as 

possible as you remove it. 

e. When scraping paint, as when found on a car, scrape down deep 

to the base material, such as the metal of the car. Collect all 

layers of paint available (all evidence layers and all matrix layers 

on which evidence rests) in one scrape. This method applies to 

all trace evidence to be scraped.

f. If ample evidence is present, attempt to collect a sample that is 

at least the size of a quarter when scraping material that is not a 

flake. Otherwise, scrape and collect all of the evidence present.

g. Scrape the material directly into a paper bindle or self-made 

envelope. A bindle or self-made envelope is preferred for 

collection to be able to recover as much of the evidence from 

inside of it as possible. 

h. Close and seal the bindle or envelope to prevent leakage. 

i. Never use staples to seal a bindle containing trace evidence.

j. Place a small section of tape at the point where the top is tucked 

into the bottom just sufficient to keep the bindle top tucked into 

the bottom. Do NOT over wrap the bindle or envelope with 

tape.

k. Place the bindle or self-made envelope into an appropriate size 

labeled container.

l. Take necessary precautions to avoid breaking or damaging larger 

flakes by affixing the bindle or envelope to a piece of cardboard 

or other rigid material. Secure the bindle or envelope to keep it 
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from moving with minimum amount of transparent tape (not 

evidence tape or packing/tape lifting tape). 

m. Fold the paper that was below the paint when scraped, and place 

it into a labeled container. Keep all evidence from the same 

scrape together using detailed labeling of the containers and 

evidence tape.

n. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

Control sample: Label a container for the control or comparison 

sample with your initials, identification number, the date and time, 

evidence number, location of the control in relation to the evidence 

sample, and a description of the control sample. Clearly identify this 

sample as a control or comparison sample (e.g., write the words 

“Control Sample” or “Comparison Sample” in bold print on the 

container). 

Control sample number: Each piece of evidence must have a 

unique number. This number should correspond to the placard next 

to the evidence and on the evidence log; e.g., “#36.A - Control 

sample for evidence #36.”

Control sample description: The description includes: 

• Type of item scraped (e.g., paint, apparent brown stain on 

glass, etc.)

• Location of the object scraped 

• Location of the scrape on the object 

Collect a control sample: Collect a comparison or control sample 

by scraping an area close to or adjacent to the transfer scraping. (Use 

a clean, new blade; never use a blade that was used to scrape another 

sample.) Collect a control or comparison sample that is, at a 

minimum, the size of a quarter. Collect a sample that cuts through all 

layers of material down to the solid or base surface, as when 

collecting a paint sample from a car. 

a. Place or tape a self-made envelope or glassine bindle below the 

area to be scraped before scraping to capture all of the scraping 

possible. Do not use a commercially manufactured envelope of 

any kind as they have gaps that permit leakage.

b. Scrape off as much of the material as possible and go as deep as 

possible to obtain all layers. 

• Note: When scraping flakes, attempt to leave the flake as 

intact as possible as you remove it.
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c. When scraping (e.g., paint, as when found on a car) scrape down 

deep to the base material, such as the metal of the car. Collect all 

layers available in one scrape if possible. 

d. Place the scraping directly into the bindle or self-made envelope.

Never package comparison or control samples with transfer 

evidence samples.

e. Place the bindle or envelope into the labeled container. 

f. When the bindle or envelope contains larger flakes, place it onto 

a rigid material similar to the evidence sample then into a 

container and secure it to prevent the bindle or envelope from 

moving. Take necessary precautions to avoid damaging the 

flakes.

g. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

h. Store the object in the sealed container. 

Treat the control or comparison sample as you would treat the 

transfer evidence sample.

Collection Procedure: Lift with Tape or Adhesive 

Follow Trace Evidence Documentation and Packaging 

Guidelines

Additional Equipment Needed 

Clear tape (packing or box sealing tape with a width of 2.5 inches to 4 

inches) on a roll (tape lift), sheet lift tape, clear acetate page protector 

or acetate sheets, clear secondary liners (non-silicone or silicone 

coated one-side), and clean butcher paper. 

 A tape lift has the advantage over vacuuming by collecting 

evidence most recently deposited relative to the crime.

 Suitable types of tape to use are packing or box-sealing tape with 

a width of 2.5 inches to 4 inches. 

 Do not use fingerprint lift tape, latent print tape strips or lifters 

unless as a last resort. While excellent for fingerprints, these 

often have insufficient adhesive for trace evidence.

 Do not use masking tape, clothing hair-removal tape, duct tape, 

or other non-transparent tapes.

 Do not use paper as a backing for tape adhesive. Use acetate 

page protectors or clear secondary liners.
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 Hair is frequently located at a crime scene near a weapon, near 

the point of impact, and below a deceased person, on clothing 

worn during the crime. 

 Fiber is frequently located at a crime scene on clothing, carpet, 

furniture, and bedding. Fiber is usually defined as carpet and 

clothing filaments. 

 Broken glass is frequently found on clothing, shoes, head hair, 

skin, tools, and weapons. 

 Paint is commonly located on the object that impacted the 

painted surface, the area below/around the painted surface that 

was impacted, and the clothing and shoes of people who have 

been in the area of the painted surface at the time of or since the 

impact.

 Do not freeze the lift. 

 To collect paint samples when the entire item cannot be 

removed, use the tweezers collection method first, then the 

scrape method, then the tape lift method. 

 Always use powder-free clean gloves when handling evidence.

 Do not cough, sneeze, scratch, or talk over any sample being 

collected or dried. 

 Whenever possible, collect the entire item and submit it to the 

lab. 

 Collect trace evidence (e.g., hair, fiber, paint, etc.) using tweezers 

when possible, then use tape lifts. 

Marking Evidence 

Evidence number: Each piece of evidence must have a unique 

identification number. This number should correspond to the placard 

next to the evidence and the evidence log as appropriate. 

Evidence labeling: Label the acetate page protector or secondary 

liner along an edge and a container for the sheet or liner with your 

initials, identification number, the date and time, evidence number, 

lift location (e.g., lower, bed sheet, left-front shirt). Label each tape 

lift with your initials, the date, and identification evidence number. As 

appropriate (e.g., when collecting a series), add additional specific 

location information to the tape end. 

On the tape, a letter may be appended to the original evidence 

number to denote the lift. If the original evidence number was #36, 

for example, the number on the bindle could be #36A or #36.1. If 

several lifts are from the same evidence item (e.g., shirt) then the 

same sub-number (e.g., #36A) can be used for all lifts on the same 
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acetate page protector or unique numbers used for each separate lift, 

according to department preference.

Evidence Description: The evidence description should include 

the:

• Type of evidence 

• Location of the evidence 

• Description of surface from which lift is taken 

• Brief description of evidence, when appropriate, such as 

“blue-colored glass” or “apparent smokeless powder” 

 On the envelope, avoid using adjectives, such as “long” or 

“blonde”, to describe hair; e.g., “Hair and other items lifted with 

tape from green area rug lying across the floor two feet east of 

the back door.” 

 On the bindle, when it is used for debris, refer to the evidence 

with which the content of the bindle is associated; e.g., “Debris 

from evidence #36.”

 Label the container just before collecting a sample, and seal the 

container immediately after collection. 

Lifting Trace Evidence 

(Tape on a roll or as used in sheets are considered “tape.” Acetate 

sheets, page protectors, and release liners will be “backing.”)

a. Remove a little longer clear tape from the roll than will be 

needed to tape lift the intended surface. Fold approximately an 

inch of tape onto itself on the ends. 

b. Place the tape evenly on the surface to be tape lifted. Press 

repeatedly and firmly along the length of the tape. Peel the tape 

off of the surface carefully so as to keep evidence from falling 

off of it. If surface pattern is important, place adhesive side of 

tape on the backing. If pattern is not important, repeatedly place 

the tape onto new areas of the surface with potential evidence 

several times, but before the tape looses stickiness. Place the 

adhesive side of the tape onto backing. 

• If lift tape is being used, open the tape lift and remove the 

protective seal over the sticky part of the lift. 

• If clear tape is being used, remove a piece of tape.

c. While holding the tape over clean paper, close the lift tape or 

place the piece of clear tape against the sheet of acetate.
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• If lift tape is being used, carefully place the backing over the 

sticky surface.

• If clear tape is being used, carefully place the tape on the 

acetate.

d. Be sure not to let any evidence fall from the tape lift or clear 

tape.

e. If the tape lift or clear tape is not sticky enough to securely 

attach to the backing or acetate, use an additional piece of tape 

to secure it to the backing. 

f. Label the tape with your initials, identification number, the date 

and time, evidence number, and location as appropriate. The 

backing should be labeled along a margin parallel to the 

direction of the tape with your initials, identification number, the 

date, evidence number, location and description 

Packaging

a. Place the tape lift into the labeled envelope. The envelope and 

tape backing should have identical information on their labels. 

b. Save any debris that fell off of the tape onto the clean paper by 

folding the paper into a bindle and placing it into an envelope. 

Take care to fold the paper in such a manner as to contain all 

debris; using a “druggist fold” to make a bindle is 

recommended. The container should be labeled in the same 

manner to match the lift envelope’s label. 

c. If a control or comparison tape lift is made, package separately 

from the evidence tape lift.

d. Close the container and seal the entire opening with evidence 

tape. Write your initials, identification number, and the date 

across the evidence tape seal.

e. Store lifts in a secure, dry storage area until they are submitted.

Collection Procedure: Recovery with Tweezers 

Follow Trace Evidence Documentation and Packaging 

Guidelines

Additional Equipment Needed 

Fresh disposable or clean, smooth-tipped tweezers. Paper/manila 

envelopes; 4-inch and/or 6-inch glassine weigh paper (for paper 

bindles or self-made envelopes); tweezers/forceps with different tips; 

waterproof pen; evidence tape; powder-free protective gloves; face 

protection 
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 Hair is frequently located at a crime scene near a weapon, near 

the point of impact, and below a deceased person, on clothing 

worn during the crime. 

 Fiber is frequently located at a crime scene on clothing, carpet, 

furniture, and bedding. Fiber is usually defined as carpet and 

clothing filaments. 

 Broken glass is frequently found on clothing, shoes, skin, tools, 

and weapons. 

 Paint is commonly located on the object that impacted the 

painted surface, the area below/around the painted surface, and 

the clothing and shoes of people who have been in the area of 

the painted surface at the time of or since the impact.

 Always use clean, powder-free gloves when handling evidence.

 Do not cough, sneeze, talk, or scratch over any sample being 

collected or dried. 

 Broken glass is frequently found on clothing, shoes, head hair, 

skin, tools, and weapons. 

 Paint is commonly located on the object that impacted the 

painted surface, the area below/around the painted surface that 

was impacted, and the clothing and shoes of people who have 

been in the area of the painted surface at the time of or since the 

impact.

Marking Evidence 

Evidence Labeling: Label a container for the bindle or envelope 

with your initials, identification number, the date and time, evidence 

number, location, and evidence description. Label the bindle or 

envelope with your initials, identification number, evidence number, 

the date, and evidence description. 

Evidence Number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence and the evidence log as appropriate. 

Evidence Description: The evidence description should include 

the:

• Type of evidence

• Location of the evidence 

• Brief description of evidence, when appropriate, such as 

“glass fragments” or “hair.” Avoid using adjectives, such as 

long or blonde, to describe objects being collected, such as 

hair; e.g., “Fibers and hair found on a denim-covered 

couch.” 
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 Label the bindle and container just before collecting an object, 

and seal the container immediately after collection. These 

actions help to protect the integrity of the sample and the chain 

of custody. 

Collecting Evidence 

a. Gently pick up the evidence using clean tweezers. Use only clean 

tweezers when collecting a piece of evidence.

b. Grasp the evidence gently and with only sufficient force to 

securely collect the evidence with the tweezers. When collecting 

hair, be sure to capture the root of the hair when possible since 

DNA is contained in the hair root. 

c. Continue using the tweezers on all evidence large enough to be 

collected and place the evidence into the bindle or envelope. Use 

and label a different bindle for different apparent types of 

evidence, different areas of collection (as appropriate), or for 

other reasons. 

d. Close the bindle or envelope to contain the evidence and protect 

it from contamination or leakage. When closing the bindle, make 

sure to contain the evidence placed into it. 

Packaging

a. Place the bindle into an envelope or other container. 

b. Close the container and seal the entire opening with evidence 

tape. Write your initials, identification number, and the date 

across the evidence tape seal.

c. Store the sealed envelope or container.

Collection Procedure: Swab a Surface 

Follow Trace Evidence Documentation and Packaging 

Guidelines

For most trace evidence, all other methods should be considered 

superior and therefore, the most appropriate ones attempted first 

before using a swab to collect evidence. Often the swab collects too 

little of the evidence or embeds the evidence within the fibers of the 

swab making removal or recovery difficult for analysis. Reasonable 

uses for a swab exist, however, such as recovery of pepper spray 

from the face of a subject or dye-pack contents from a vehicle 

interior or hands of a subject. 

Marking Evidence 

Evidence labeling: Label a container for the swab with your initials, 

identification number, the date and time, evidence number, location, 

and evidence description. 
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Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence and the evidence log as appropriate. 

Evidence description: The evidence description includes:

• Type of evidence

• Location of the stain 

• Whether the stain is wet or dry

When describing stains, use the word “apparent” or the phrase “of 

unknown origin” when the source of the stain is unidentified; e.g., 

“Brown stain of unknown origin on bathroom floor.” 

a. Label the container just before collecting the swab, and seal the 

container immediately after collection. These actions help to 

protect the integrity of the evidence and the chain of custody. 

b. If the stain is dry, moisten the cotton tip of a swab using an 

appropriate solvent depending on the evidence such as two or 

three drops of distilled water. Do not saturate the swab with 

solvent, just enough to “dampen” the swab with solvent. To 

avoid contamination, do not touch the cotton tip of the swab to 

any surface other than the sample area. Also ensure that the 

portion of the area or object with the stain has been 

photographed and documented in notes before proceeding. 

c. Hold the bottle of solvent above the swab. Use a minimum 

amount of solvent to moisten the swab: drop two or three drops 

of solvent on the swab. 

• Do not touch the tip of the water bottle or transfer pipette 

to the swab. 

• Do not saturate the swab. (It should be moist, but not 

dripping.) 

d. In some instances, distilled water may not place the stain in 

solution so a different solvent such as rubbing alcohol may need 

to be used. Consult with your local crime laboratory personnel 

for specific procedures to use. 

e. Swab the stain with the cotton-tipped end of the swab. Touch 

the swab gently and firmly to the stain. Rotate the swab to 

ensure that the stain is collected on as much of the cotton tip as 

possible. Do not smear the stain when swabbing it. 

f. Dry the swab.

g. Place the swab into a container (e.g., bindle, self-made envelope, 

or drying box). If necessary, break off the end of the swab so the 

swab fits. Then, close the bindle and place it into a container. 
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Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number and the date 

and time across the evidence tape seal.

Control sample: Label the control container with your initials, 

identification number, the date and time, evidence number, location 

of the control in relation to the original sample, and description of 

the control sample. Clearly identify this sample as a control sample 

(e.g., write the words “Control Sample” in bold print on the bindle). 

Control sample number: Each piece of evidence, including the 

control sample, must have a unique identification. A letter or number 

may be appended to the original evidence number to denote the 

control sample; e.g., If the original evidence number was #32, the 

control sample could be #32.A or #32.1.

Control sample description: The description includes: 

• Description of the stain – what is being collected.

• Location of the control sample in relation to the stain 

evidence 

• Location of the original stain 

Label the container just before collecting an object, and seal the 

container immediately after collection. 

Collect a control sample: Moisten the cotton tip of the control 

swab(s) using the same solvent as the evidence. Hold the bottle of 

distilled water or transfer pipette containing solvent above the swab 

and drop two or three drops of water or solvent on the swab. 

 Do not touch the tip of the transfer pipette with solvent to the 

swab.

 Do not saturate the swab. (It should be moist, but not dripping 

wet or saturated.)

a. Locate an unstained area of the same surface from which the 

evidence stain sample was taken. Swab the unstained area of this 

surface.

• Touch the swab gently and firmly to the stain. 

• Rotate the swab to ensure that the stain is collected on as 

much of the cotton tip as possible. 

b. Allow the swab to dry while continuing evidence collection. 

Place the dry swab into a bindle or self-made envelope. Then, 

place the bindle into a manila or other envelope. Close the 

envelope and seal the entire opening with evidence tape. Write 
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your initials, identification number, and the date across the 

evidence tape seal.

Treat the control sample as you would treat the evidence 

sample. 

Do not package control or comparison samples with evidence 

samples.

Collection Procedure: Soil and Rock Samples 

Follow Trace Evidence Documentation and Packaging 

Guidelines

Additional Equipment Needed 

Compass; measuring tape; gardener’s hand shovel; mason’s trowel; 

screwdriver.

 Always use clean tools and individual bindles when collecting 

soil or rock samples.

 If an impression, print, body or other evidence is in the area, 

photograph and document it in notes before collecting nearby 

soil or rocks. 

 Collect samples from the known crime scene, any “alibi” site(s) 

(e.g., a site that the victim or accused claims to have visited), or a 

“representative” site (such as a site where prints that match 

recovered shoes or tires are found). 

 When soil is firmly attached to a movable object, collect and air 

dry the object before packaging it. If it cannot be collected, 

gently scrape samples from the object onto clean paper.

 Never package soil directly into commercially manufactured 

envelopes or bags. 

 Always package soil in a sealable container: glass or plastic vials, 

bottles or jars with screw cap lids, self-made envelope, paper 

bindle, or other container. 

 Collect minimum of three tablespoons of soil from each 

location. Go a little deeper than at least as deep as the evidence 

sample appears to have penetrated the ground soil. Usually the 

top layer of soil is only disturbed for the evidence sample. 

 Collect a comparison sample close to the suspected or known 

evidence sample location and at various locations around the 

evidential sample up to 100 feet, attempting to include varieties 

of soil in the scene area. (See Figure D-14.)
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 Layering of soil can be important to show recent or historical 

presence at a location. This is particularly true of vehicle 

collection. Preserve layering whenever possible. 

Documentation

Ensure that the portion of the object with the soil has been 

photographed and documented in notes and sketching. Include 

measurements of collected soil (evidence or control/comparison 

samples) locations. 

Marking Evidence 

Evidence labeling: Label a container for each soil sample with your 

initials, identification number, the date and time, evidence number, 

location, and evidence description. Label each bindle, envelope, 

bottle, or jar with your initials, identification number, evidence 

number, the date and time, location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence (evidence, control, or comparison) and the evidence log as 

appropriate. 

Evidence description: The evidence description includes:

• Location of each soil sample relative to specific landmarks 

at the scene

• Whether the soil was wet or dry when collected

• Whether the soil contains any detectable odor or other 

unusual characteristics or objects

• Estimated amount of sample

When describing soil contents, use the word “apparent” or the 

phrase “of unknown origin” when the source of the stain/mark is 

unidentified; e.g., “Soil sample taken from approximately 1 foot 

north of apparent boot print (#6A.1).” 

a. Determine where to begin sample collection.

1. If a victim died on the ground, and the body has not been 

removed, collect the sample from as close to the body as 

possible without disturbing it. Otherwise, prepare to collect 

a sample from the center of where the body laid and other 

appropriate locations of the body. 

2. If collecting a series of samples along the path of an 

impression, determine the start and end points of the path. 

The starting point is the place where the first impression is 

made and the first sample must be collected, then along the 

path traveled by the subject. 
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3. Examine the ground for trace evidence that may not have 

been collected. 

b. Dig straight down into the soil/rock to collect a sample of three 

tablespoons to one cup of the soil. 

 Be sure to start with clean digging tools. Clean the tools after 

each sample. Use the mason’s tool, gardener’s hand trowel, 

and screwdriver as needed to dig straight into the ground or 

rocks.

c. Place the soil sample into the container (bottle, jar, vial, bindle).

 Mix soil as little as possible, keeping in mind the potential 

for layering of soil.

 To avoid contamination and leakage, it is critical that each 

sample is stored in its own sealed container and kept apart 

from other soil/rock samples and tools that were used. 

d. Collect and package the remaining samples in separate bindles. 

Take additional samples at distances of 1, 10, 25, and 50 feet 

from the original impression/impact point. [recreate the diagram 

below] Size of area comprising the scene will determine how far 

out the samples need to be taken. 

Figure C-14. Collect soil and rock samples at regular distance 

intervals from the original impression. 
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 Take at least four samples at varying compass points each 

time the distance from the initial evidence area and sampling 

location is increased.

 Ensure that your notes and the label on the container 

include the compass direction and distance from the 

previous location, or the initial evidence sample, or 

permanent markers used for measurement in the scene. 

Whichever reference point is used, consistently use that 

point throughout. 

e. If the sample is wet, place it on a clean piece of paper in a secure 

location used for evidence drying, such as a drying rack, until it 

is dry.

f. If drugs or ignitable liquids are suspected, the sample must 

be frozen. Contact a fire investigator or fire debris analyst if 

ignitable liquids are suspected. 

 Keep the sample as cold/cool as possible helps to slow 

degradation. Natural components of some soils may degrade 

the composition of added chemicals in the sample. 

Packaging

Place each initial container (bottle, jar, vial, bindle) into its labeled 

container. Only one soil sample per container. Do not package 

evidence with comparison/control samples. 

Close the container and seal the entire opening with evidence tape. 

Write your initials, identification number, and the date and time 

across the evidence tape seal.

Gunshot Residue Collection Methods 

Follow Trace Evidence Documentation and Packaging 

Guidelines

Equipment Needed 

Gunshot residue (GSR) collection kit or GSR collection stubs; paper 

envelopes; paper towels; clean paper; waterproof pen; evidence tape; 

powder-free protective gloves; face protection.

 Determine if the suspect(s), victim(s), or witness(es) should be 

tested; collect the GSR as soon as possible. 

 Do NOT use tape lifts in place of a GSR kit stub.

 The instructions included in the GSR kit should always be 

followed. 

Courtesy Jack Dillon
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 Sample the hands for GSR using the collection kit as soon as 

possible. If collection cannot be made immediately on contact 

with the subject, individually bag the subject’s hands using paper 

bags and not plastic bags (as plastic may cause hands to sweat). 

 Good GSR samples can generally be obtained from the web 

portions of the hands. 

 GSR collection kits should contain materials (e.g., carbon-coated 

adhesive stubs or adhesive-coated discs) required to perform 

scanning electron microscopy (SEM) residue tests. 

 Do not use GSR kits that have swabs or color tests. 

 Do not allow the suspect(s), victim(s), or witness(es) to wash 

their hands or subject the hands to any liquids after the 

shooting, or any rubbing onto other surfaces (e.g., clothing, bag 

on hand, furniture, etc.). 

 Keep GSR kits away from firearms evidence. 

 Evidence such as vehicles can also be tested for the presence of 

GSR, by using the same procedure as would be used on hands. 

Footwear and Tire Impressions4 

Tire Impression Evidence 

 A vehicle may be associated or disassociated to a crime scene by 

tire impressions. 

 A comparison of the accident/crime scene impressions can 

result in an identification, inclusion or elimination of a tire. 

 Impression(s) can be found in a variety of substrates including 

soil and snow, as well as on cement and asphalt. 

 The evidentiary value of a comparison usually depends upon the 

quality of the impression and the manner in which it is 

documented and collected. 

Casting Considerations 

The decision to cast is affected by the conditions of the substrate that 

the impression is in or on. Impressions in fine, humus soil, wet sand, 

and even snow are often excellent candidates for casting. Coarse 

substrates may not always the best substrate for retaining detail of the 

tire impression(s). It is recommended that all impressions are 

 

4 See also Guide for the Collection of Footwear and Tire Impressions in the Field, 

http://www.swgtread.org/images/documents/standards/published/01_field_collection_03-2005.pdf. 

Accessed February 28, 2012.
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impressions are photographed and cast, to recover the maximum 

amount of impression detail. 

Documentation5

Photography is a valuable way of collecting impression evidence for 

later comparison. As with all evidence, overall photographs should be 

taken using a standard-format lens showing the impressions in 

relation to the other features of the scene. It is critical that distortions 

are minimized by adhering to the following: 

 

 Impression photography requires the use of a tripod and 

detachable flash.

 Documentation must include a photograph with a measurement 

scale. The scale should not be placed over or across the 

impression. The scale must be level with the bottom of the 

impression and be approximately the same size as the 

impression for proper documentation. The scale should contain 

case identification information. 

• Case number 

• Orientation

 Documentation should indicate the direction of travel, if this can 

be determined. 

 The camera should be mounted on a tripod directly over the 

impression, with the film plane parallel to the impression. 

 The impression should be shaded from direct sunlight. 

 It is recommended that the detachable flash or other light source 

be at an angle of 45 degree or less depending on the depth of 

the impression. A variety of flash angles are recommended. 

These oblique light photographs should be taken with the 

direction of the flash coming from at least 3 different directions 

around the impression. 

 Tire impressions are photographed in an overlapping series that 

should continue the length of the impression in which detail is 

present. 

• Each frame should overlap by approximately 20%, and no 

more than two feet should appear in each frame. A scale or 

 

5 See also General Guidelines for Photographing Tire Impressions, 

https://www.swgit.org/pdf/Section%209%20General%20Guidelines%20for%20Photographing%20Tire%20Impressio

ns?docID=53. Accessed February 29, 2012
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tape measure placed the length of the track will help 

reconstruct the length of the entire impression from the 

separate photographs. 

Single-lens reflex or similar cameras with changeable lenses 

should be used for capturing impression evidence. 

Tire Vehicle Measurements 

The following measurements should be recorded: 

 The track width of a vehicle is the distance between the 

center of the tire mounted on one side of the vehicle to the 

center of the tire on the opposite side. The front and rear 

track widths may be different. 

 The wheelbase of a vehicle is the distance between the 

center of the front axle and the center of the rear axle. 

Tire Impression Measurements 

Measure the track width and wheelbase recorded in the impressions, 

if possible: 

 The track width may be measured from the inside of one 

tire track to the outside of the adjacent tire track, if they can 

be determined to be a pair made by a single vehicle. 

 If the positions of the front and rear tires can be 

determined where a vehicle stopped, these positions can be 

measured to determine an approximate wheelbase 

measurement. 

Casting (see Cast an Impression) 

After photography, casting may be performed to document the 

impression in three-dimensional form. The decision to cast is 

affected by the substrate conditions and other environmental factors. 

 Impressions should be photographed before casting. 

 Do not remove soil adhering to the cast or attempt to clean the 

cast after recovery as this may damage cast detail. 

 Place each casting in a protective, breathable container after 

drying. 

Enhancements/Optimization 

Chemicals and/or powders may be used to enhance or optimize 

impression(s). It should be determined prior to chemical application 

if a sampling of the blood is required, as the chemicals used to 

optimize the impression(s) may interfere with DNA analysis. 
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 Forensic light sources may optimize visualization and 

photography of the impression(s). 

 Examination of the impression(s) using a forensic light source 

may be conducted prior to applying any chemicals or powders. 

 Blood does not fluoresce, but views as black in infrared (IR)

range and may offer contrast between the impression and the 

substrate of the item it is on.

Footwear Impression Evidence 

 A shoe or boot may be associated or disassociated to a crime 

scene by footwear impressions. 

 A comparison of the crime scene impressions can result in an 

identification, inclusion or elimination of a footwear outsole. 

 Impressions can be found in soil, snow, on counters, tile floors, 

doors, wood and vinyl furniture, paper items, as well as other 

surfaces.

The decision to cast an impression is affected by the conditions of 

the substrate the impression is in or on. Impressions in fine, humus 

soil and even snow are often excellent candidates for casting. Coarse 

substrates may not always be the best substrate for retaining detail of 

the tire impression(s). It is recommended that all impressions are 

photographed and cast, to recover the maximum amount of 

impression detail. 

Documentation (also see Photography) 

Photography is a valuable way of collecting impression evidence for 

later comparison. The evidentiary value of a comparison usually 

depends upon the quality of the impression and the manner in which 

it is documented and collected. 

Overall photographs should be taken using a standard-format lens 

showing the impressions in relation to the other features of the scene. 

It is critical that distortions are minimized by adhering to the 

following: 

 Impression photography requires the use of a tripod and 

detachable flash.

 Documentation must include a photograph with a measurement 

scale. 

 The scale should not be placed over or across the impression. 

 The scale must be level with the bottom of the impression and 

be approximately the same size as the impression for proper 

documentation. 

Courtesy of Rebecca Carter
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 The scale should contain case identification information: 

• Case number 

• Orientation

 The camera should be mounted on a tripod directly over the 

pattern, with the film plane parallel to the impression. 

 The impression should be shaded from direct sunlight for flash 

photographs. 

 It is recommended that the detachable flash or other light source 

should be at an angle of 45 degrees or less depending on the 

depth of the impression. A variety of flash angles are 

recommended. These oblique light photographs should be 

taken with the direction of the flash coming from at least 3 

different directions around the impression. 

 The entire impression should be captured in one frame unless 

overlapping photographs are needed to capture sufficient 

resolution. 

Single-lens reflex or similar cameras with changeable lenses 

should be used for capturing impression evidence. 

Casting (see Cast an Impression) 

After photography, casting may be performed to document the 

impression in three-dimensional form. The decision to cast is 

affected by the substrate conditions and other environmental factors. 

Impressions should be photographed before casting. 

• Do not remove any soil adhering to the cast or attempt to 

clean the cast after recovery as this may damage cast detail.

• Place each casting in a protective, breathable container after 

drying. 

Two-dimensional Impressions

Two-dimensional impressions are usually a deposit or removal of 

material to or from a surface. These may be found on paper items, 

doors, counters, tile floors, and other hard surfaces/substrates. There 

are generally two ways footwear impressions are made:

• by the removal of dust or other material from a surface by 

adhering to an outsole leaving a void (negative) impression 
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• the deposition of a material or contaminate such as blood, 

dirt, and oil present on a footwear outsole, transferred to a 

surface, leaving an impression

If possible, submit the entire item that has the impression on it. If 

that is not practical, the impressions may be lifted using various 

techniques such as: 

• Electrostatic dust lifter

• Gel print lifter

• Tape or clear adhesive material (if no other material is 

available)

Enhancements/Optimization6

Chemicals and or powders may be used to enhance or optimize 

impression(s). It should be determined prior to chemical application 

if a sampling of the blood is required, as the chemicals used to 

optimize the impression(s) may interfere with DNA analysis.

 

Forensic light sources may optimize visualization and photography of 

the impression(s). Examination of the impression(s) using the 

forensic light source may be conducted prior to applying any 

chemicals or powders. It should be noted that blood does not 

fluoresce, but views as black in IR range and may offer contrast 

between the impression and the substrate of the item it is on. 

Collection Procedure: Cast an Impression7

Casting an impression should only be attempted by someone trained 

and experienced in the technique employed. 

 

Equipment Needed 

Casting kit (dental stone); mixing container (such as a heavy duty 

baggie or a bucket); stirring stick; material for forms; water; packaging 

materials; waterproof pen; evidence tape; measuring tape/ruler; 

identification labels; protective gloves; face protection.

Documentation

Properly photograph the impression before casting. 

 

6 See also SWGTREAD Guide for the Detection of Footwear and Tire Impressions in the Field, 

http://www.swgtread.org/images/documents/standards/published/03_field_detection_03-2005.pdf. 

Accessed February 28, 2012.

7 See also 

http://swgtread.org/images/documents/standards/published/01_field_collection

_03-2005.pdf, accessed June 18, 2012.
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Marking Evidence 

Evidence labeling: Label a container for the object with your initials 

and identification number, the date and time, evidence number, 

location, and evidence description. 

Evidence number: Each impression must have a unique number. 

This number should correspond to the placard next to the evidence. 

Evidence description: The evidence description includes:

• Type of item being cast

• Location of the item being cast

• Orientation of the impression to north, to a feature of the 

object with the impression on it, or to a nearby object 

Casting the Evidence

 When making a cast, be prepared to act quickly and 

methodically. Time is often a critical factor in successfully 

making a cast.

 When casting tire impressions, cast as much of the tire 

impression as possible. 

 Use dental stone for casting in dirt, soil and sand as well as 

snow. Sulfur may be used for casting in snow. 

 Impression coating materials such as SnowPrint Wax, stabilizers 

(e.g., hairspray) and highlighters (e.g., paint) may be used 

depending upon conditions present. 

 Casts should be marked with the impression identifier, general 

evidence collection information (date, case number, etc.) and 

with a directional indicator.

 Casting material should be allowed to thoroughly harden before 

removal from surface. 

 Prepare the casting material. Follow the manufacturer’s 

instructions for preparing the casting material. 

 A sturdy plastic bag is used for mixing dental stone for footwear 

impressions, a bucket is usually necessary for mixing casting 

material for tire tracks.

 The initial pour of the casting material should occur off the 

impression to avoid damaging detail; the casting material should 

be allowed to flow into the impression. 

Casting impressions with dental stone in soil and sand:

a. If necessary, prepare impressions for casting:
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1. When casting a fragile impression, it may be necessary to 

apply a fixative. Care should be exercised when applying 

fixatives to minimize any possibility of damage to the 

impression. 

2. When casting in dense soils, it may be necessary to apply a

release agent. Care should be exercised when applying 

release agents to minimize any possibility of damage to the 

impression. 

b. Add appropriate amount of water to pre-measured amount of 

dental stone. The average footwear impression requires 

approximately two (2) pounds of dental stone and approximately 

ten (10) ounces of water. The amount of water required may 

vary depending on the casting product. The resulting mixture 

should have the viscosity of heavy cream. The viscosity of the 

mixture may need to be adjusted based upon the nature of the 

impression. 

c. Mix continuously for a minimum of 3-5 minutes so that the 

powder can thoroughly absorb the water. 

d. Pour casting material carefully outside the perimeter of the 

impression and direct the flow into the impression. Ensure the 

impression is completely filled and/or covered evenly. In the 

event that the casting material does not flow completely into the 

impression, the top surface of the casting material can be 

agitated to help it flow. Casts should be of sufficient thickness to 

avoid breakage. If necessary, additional casting material may be 

poured over the top of the original cast to complete the cast 

and/or add thickness. 

For fragile and shallow impressions: pour casting material 

from outside the perimeter so that it rapidly flows over the 

impression. A thinner mixture of casting material is necessary 

for this technique. Avoid pouring directly onto the uncovered 

impression. 

Note: 

 Larger quantities of dental stone can be mixed in a bucket to 

cast large segments of tire impressions. 

 Impressions under water may be cast using dental stone and 

specialized techniques.

Casting impressions with dental stone and sulfur in snow:

a. If necessary, prepare impressions for casting. It is noted that 

snow varies considerably in texture and type. Application of 

highlighting materials (such as Snow Print WaxTM or aerosol 
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paints) may be advantageous during photography. These 

materials may or may not be necessary for the casting process.

b. To increase the contrast of the detail, a thin application of 

highlighting spray may be directed at the impression from an 

oblique angle. The application of highlighting sprays to the snow 

impression may increase melting; therefore, the impression may 

need to be shielded from the sun until it can be photographed 

and cast.

c. A thick application of SnowPrint WaxTM may be applied if 

needed before using the dental stone casting material.

d. Casting with dental stone: 

1. Add a heaping tablespoon of Potassium Sulfate to the preweighed bag of dental stone.

2. Add snow to the water source and place the bags of dental 

stone in the snow to pre-cool the ingredients. 

3. Add the appropriate amount of water to the pre- measured 

dental stone. A thicker mixture should be used for snow. 

4. Pour the casting material from outside the perimeter and 

direct the flow into the impression. The surface of the 

casting material can be agitated to help it flow.

e. Casting with sulfur: 

1. Snow impressions may be cast using sulfur; however, it is 

recommended that specific training in this technique be 

acquired before using. 

Packaging

a. Collect and package debris that may have fallen from the cast 

when it was removed. 

b. Store the packaged debris with the cast.

c. Do not clean the cast. 

d. Package the debris in a bindle or other container that will 

securely store it. Label the container with your name, 

identification number, and the date and time. Place the cast into 

the labeled container. When necessary, use shock absorbing and 

protective materials to cushion the cast.

1. A cast is delicate and can be easily damaged. Package the 

cast so that it is stable and secure and the face of the 

impression is up and protected from disturbance during 

transport. 
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2. If a cast is too large to box, wrap it in clean paper and 

shock-resistant material (such as bubble-wrap). The goal is 

to seal the impression and protect it from damage during 

transport. 

e. Never discard a cast, regardless of condition when removed 

from the impression. Be sure to save and submit all casts to the 

laboratory. Even a broken cast may be useful during the 

examination process. 

f. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

g. Make sure that the container is labeled with a description of the 

item cast, your initials and identification number, the date and 

time, location and, when possible, evidence number. 

Collection Procedure: Lifting Dust Footwear Impressions8

Equipment Needed 

 

Fingerprinting kit (fingerprint powder, soft-bristled brush, lifting 

tape); contact paper and clear acetate; electrostatic dust lifter; 

adhesive and gelatin lifting materials; MikrosilTM or other 

polyvinylsiloxane (PVS) casting materials; flashlight and other 

available light sources; clean wrapping paper; envelopes; waterproof 

pen; evidence tape; measuring tape/ruler; identification labels; 

protective gloves; face protection.

 Photograph the impression before collecting or removing it. 

 An electrostatic dust lifter is appropriate to use only when the 

impression is left in dry dust. 

 Whenever possible to do so without damaging the impression, 

collect the object containing the impression. 

 Always use clean gloves when handling evidence.

 Always collect soil/rock samples from the immediate 

surrounding area when the impression is on the ground. 

Documentation

Properly photograph the impression before lifting. 

 

8 See also 

http://swgtread.org/images/documents/standards/published/12_lifting_03-

2007.pdf Accessed June 20, 2012.
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Marking Evidence 

Evidence labeling: Label a container and an identification label for 

the developed impression with your initials and identification 

number, the date and time, evidence number, location, and evidence 

description. 

Make sure that the information on the container matches the 

identification labels.

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes: 

• Type of print collected

• Location of the impression 

• Orientation of the impression to north, or to a feature of 

the object with the impression on it, or to a nearby object 

Label the container just before collecting an object, and seal the 

container immediately after collection. 

Lifting the Impression 

When lifting impressions, examiners should use the least destructive 

method first. If in doubt, treat all impressions as dry-origin and apply 

the methods listed below. If unsuccessful, attempt collection as 

indicated for wet-origin impressions. All procedures shall be 

performed when applicable and noted when appropriate. The order 

and use of these individual techniques is determined by 

considerations such as substrate, components of the impression, and 

environmental conditions. 

Electrostatic dust lifter 

Electrostatic lifting is useful for the detection and lifting of dry-origin 

dust and residue impressions that are the result of tracking from dry, 

dirty surfaces onto relatively cleaner surfaces. Electrostatic lifting is 

normally the first technique used, as unsuccessful attempts will not 

prevent subsequent lifting and enhancement techniques. There are a 

number of electrostatic lifters available. Consult the manual provided 

by the manufacturer for specific operating instructions. 
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All of these devices utilize a film which has a black side and an 

aluminum-coated side. The black side of the film is placed against the 

impression, and a high-voltage charge is applied to the film, resulting 

in the transfer of the dry dust or residue impression. 

 To visualize the lifted impressions, the lifts should be examined 

in a darkened room with a high-intensity light source held at an 

oblique angle to the surface of the lift. 

 The lifting film should never be reused.

 Smaller lifts can be stored in individual clean file folders. These 

folders should never be reused. 

 Larger lifts can be stored by carefully rolling with the aluminum 

side out. After rolling, the edge can be secured with a small piece 

of tape. 

 Electrostatic lifts are fragile and impressions can be destroyed by 

any wiping action across the surface of the lift. Consideration 

should be given to photographing lifts prior to packaging. 

Electrostatic lifts retain a charge and should never be packaged 

in cardboard, cardboard boxes, or plastic bags. 
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Adhesive and gelatin lifters 

 Footwear-size adhesive and gelatin lifters are used for the lifting 

of dust and residue impressions, wet-origin impressions, as well 

as impressions developed with fingerprint powder. 

 Gelatin lifters are available in white, black and clear. White lifters 

provide greater contrast with impressions enhanced with darkcolored powders. Black lifters provide greater contrast with 

light-colored powders and residue impressions. Clear lifters 

normally do not provide good contrast. Gelatin lifts of residue 

impressions should be photographed as soon as possible after 

collection. 

 Adhesive lifters are available in white and clear. They include 

footprint-sized sheets and various widths of rolled tapes. White 

backgrounds are recommended for clear adhesive. Clear 

adhesive on a clear background is not recommended for residue 

impressions, these lifts are normally used for impressions 

developed with dark-colored powders. Residue or powdered 

impressions may also be lifted with tape if other lifting material 

is not available. Sections of tape may be overlapped and lifted as 

a single lift to recover the entire impression intact. The tapelifted impression should be placed on a contrasting or white 

background. Rolled tapes are available in five-inch widths and 

are preferred to narrower tapes.

 Dental Stone can be used to lift impressions such as mud and 

tire residues from surfaces such as concrete and tile. Refer to the 

“Guide for Casting Footwear and Tire Impression Evidence” 

for mixing instructions. A thick layer of dental stone can be 

poured over the impression area and lifted when dry. Note that 

a border of cardboard or other material should be placed around 

the impression to aid the lifting of the dental stone after drying. 

 MikrosilTM or other polyvinylsiloxane (PVS) casting materials 

can be used to lift impressions enhanced with powder, from any 

surface. These products lift the complete powdered impression 

and are particularly useful on textured surfaces.

Packaging

a. Insert the lifted impression into the labeled envelope. 

b. Do not bend or fold the lifted impression to fit it into the 

envelope. 
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c. Close the envelope in which the print is stored and will be 

transported, and seal the entire opening with evidence tape. 

Write your initials and identification number, and the date and 

time across the evidence tape seal.

Motor Vehicles 

Procedure: Examine a Motor Vehicle 

Equipment Needed 

Boundary markers (rope, tape, cones, etc.); clean paper bags, boxes or 

envelopes; evidence tape; waterproof pen.

 Process a vehicle with the same caution and detailed approach as 

you would any crime scene. Obtain warrants as you would other 

crime scenes if required. 

 Establish well-defined boundaries around the vehicle using 

boundary markers (such as rope, tape, cones, etc.) to preserve 

evidence.

 Photograph the interior and exterior of the vehicle; including the 

vehicle identification number (VIN). Consider exterior 

photography from all four corners of the vehicle, towards the 

center of the vehicle.

 Collect DNA, fingerprint or trace evidence before moving the 

vehicle to avoid damaging or losing it when the vehicle is 

moved. 

 Examine exterior surfaces for latent prints including the side 

mirrors, fenders (when a wheel is missing), and the six-inch-wide 

areas surrounding the sides, hood, trunk, and roof support post. 

 Examine interior surfaces for latent prints including door 

handles, rear-view mirror, seat belt buckles, windows and 

window handles/buttons, stick shift knob, and glove box door. 
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 Tow the vehicle to a well-covered, dry, secure area, such as a 

police compound, when a detailed search for evidence is 

required. 

• Take steps to ensure that any evidence that can fall from 

the vehicle during towing can be retained; e.g., placing a 

tarp below the car or stabilizing bullet holes in glass with 

tape.

 Maintain and continue to protect the boundary around the 

vehicle until it is towed.

Documentation

Record critical information about the vehicle before it is moved. 

Information to record in your notes includes: 

• Odometer reading 

• Gas level

• Apparent damage 

• State of windows, head and tail lights, turn signal lights and 

mirrors

• Ambient temperature and radiator/hood temperature 

• Possible reason for vehicle being at the location

Whenever appropriate, wrap all stray vehicle parts related to the 

vehicle in clean paper. Lay the object on the paper and fold the paper 

around it. 

Marking Evidence 

Evidence labeling: Label the container for the object with your 

initials and identification number, the date and time, evidence 

number, location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of item

• Location of the item found 

• The proximity of the item to the vehicle 

Label the container just before collecting an object, and seal the 

container immediately after collection.
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Packaging

a. Place the wrapped object into the labeled container. Some 

objects, such as tires, may be transported without placing into 

another container. If the object will not fit into a container, seal 

the paper wrapping the object with evidence tape. 

b. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

c. Seal the openings of the vehicle with evidence tape. It is 

important to seal the vehicle by taping exterior parts of the 

vehicle that can be opened with evidence tape. 

d. Avoid applying tape to areas that might be or are known to 

contain evidence that could be damaged when touched. When 

the weather is inclement, cover the necessary areas with a clean, 

weather-resistant material, such as plastic.

e. The vehicle should be accompanied to a secured storage facility. 

f. When the vehicle arrives at its destination, sign the evidence log 

to verify that the vehicle was checked into the storage facility, 

then check that all seals on the vehicle are intact.

g. When seals are broken, note when the break occurred and 

reapply evidence tape to secure the vehicle.

Electronic and Digital Evidence9

Computers and other electronic devices are often found at crime 

scenes and may contain evidence of criminal wrongdoing. This 

evidence includes: 

 

• Media 

• Networked computers 

• Non-networked computers 

• Wireless phones and tablet computers 

• Other devices

Procedure: Loose media 

Electronic media, in this context, are objects on which digital data 

can be stored. They include storage media such as hard drives, 

compact disks (CDs), digital video disks (DVDs), “floppy” disks, 

audio and video cassette tapes, and USB flash drives. They also 

 

9 See also Best Practices for Computer Forensics,

https://www.swgde.org/documents/Current%20Documents/2006-07-

19%20SWGDE%20Best%20Practices%20for%20Computer%20Forensics%20v2.1 Accessed 11-14-

2012
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include objects like smart cards (such as some credit cards) and 

memory cards such as Secure Digital (SD) cards, Mini or Micro SD 

cards, Compact Flash Cards, and xD cards. Common brand names 

are Kingston®, SanDisk® and Lexar®. 

Equipment Needed 

Anti-static bags, bubble-wrap and other packing materials; clean 

paper bags, boxes, or envelopes; evidence tape; flashlight; waterproof 

pen; labels; personal protective equipment. 

Precautions for seizing: 

 Electronic evidence is fragile and sometimes includes timesensitive data of investigative value that is stored on or 

transmitted by electronic devices.

 Handle electronic evidence carefully because it is fragile. It can 

be easily damaged by heat, cold, shock or magnetic fields. 

 Do not handle contacts, bend connections, and expose media to 

extreme heat/cold or magnetic fields.

 Do not use destructive techniques, such as applying 

fingerprinting chemicals while collecting the electronic evidence.

 Do not try to view the contents of electronic evidence without 

assistance from a qualified analyst. 

Determine whether electronic media is present; it may or may not be 

associated with the visible computer or other electronic equipment, 

such as DVD or video player. Electronic media is small, can be easily 

hidden, and is sometimes camouflaged to appear as a non-electronic 

object. 

Use these methods to locate media: 

• Interview people at the scene or informants. 

• Examine monitors, keyboards, and mice for media slots. 

• Examine key rings, desk mat/pad, game machines (Xbox®, 

PS2TM) and telephones for media. 

• Search vehicles, toys, cameras, TVs, and any personal 

electronic device.

• Look for common types of media, such as DVDs, CDs, 

cassette tapes in drawers, boxes, closets.

• Look for smart cards (card appearance is similar to a credit 

or ATM card), especially when different names are on 

several cards found together. 

• Examine desk areas completely for usernames, passwords, 

and email accounts. 
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Fulfill the legal requirements necessary for seizure of electronic media 

by contacting the appropriate authorities to obtain legal advice and 

warrants, as needed. 

To determine legal requirements, follow departmental regulations 

while establishing whether there is probable cause to collect:

• Hardware

• Software

• Data

• Cell Phones 

Identify whether a warrant is needed or there is an exception to the 

warrant requirement.

When the media is inside of a computer or other electronic 

component, do not remove it without the assistance of an electronic 

evidence collection expert. When the state of the computer or other 

electronic component is disturbed: 

• Data can be lost or damaged.

• Files can be modified (e.g., creation dates/times).

• The computer can be damaged.

• Legitimate business can be disturbed. 

• Liability for officer and department can be created.

Documentation

When the media is readily accessible, document it as it was found 

before collecting it. Photograph the media as found using standard 

crime scene photographic techniques (overall, mid-range and closeup). 

Photograph all connections (network cables, power cables, and 

peripheral cables) to the device as you found them. 

Sketch a diagram of the placement of the media in the area and 

relative to devices, equipment, and objects. Sketch and label the cable 

connections to the device prior to collection. 

Record information that supports the photograph, including: 

• Type of media 

• Media location

• Media appearance/condition, including visible damage or 

other characteristics

• Media storage capacity (e.g., SanDisk® 256MB)

• Serial or other identifying numbers 
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Marking Evidence 

Evidence labeling: Prepare a label and apply it to the electronic 

media prior to packaging if practical. Include your initials and 

identification number, the date and time, evidence number, location 

and evidence description. Do not obscure serial numbers or 

manufacture’s labels with the evidence label. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the numbered placard 

photographed and diagramed next to the evidence. 

Evidence description: The evidence description includes:

• Media type

• Media location

• Media appearance/condition, including visible damage or 

other characteristics

• Media storage capacity

• Serial or other identifying number 

Handle electronic evidence with care. Do not use destructive 

techniques, such as applying fingerprinting chemicals, while collecting 

the electronic evidence. Do not expose electronic media to extreme 

heat, cold, or magnetic fields. 

Packaging

a. Package media to prevent the loss of data. Protect it from shock, 

bending, tearing, scratching and magnetic exposure. Use plastic 

bubble-wrap or foam pads as packing material when original 

packing materials are not available. Do NOT use Styrofoam as it 

creates static charges.

b. When the media requires fingerprinting, do not wrap it. 

c. Place the media into the labeled container.

d. When transporting electronic media, attach a label indicating: 

1. FRAGILE

2. SENSITIVE ELECTRONIC MEDIA

3. KEEP AWAY FROM MAGNETIC FIELDS

e. Close the container and completely seal the opening with 

evidence tape. Write your initials, identification number, and the 

time and date across the evidence tape seal.

f. Store electronic media in a secure area in a controlled climate 

away from magnetic sources, dust, and other contaminants. 
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g. Electronic media can be damaged when stored in areas where 

temperatures and humidity vary significantly.

Procedure: Networked Computers 

The seizure of networked computers presents unique challenges. 

Always consult with a qualified computer forensic analyst or network 

administrator before shutting down and seizing a networked system. 

If possible, work with the administrator of the system you are trying 

to seize. He/she will be the best resource to safely access and image 

media. Due to the size and complexity of modern computer 

networks, seizure is frequently not possible. Be prepared for 

extended time on site to logically or physically image media. 

When dealing with networked devices the amount of time it takes to 

secure the network and data is critical. By design many of these 

devices can be accessed from offsite and critical evidence can be 

remotely destroyed, copied, or encrypted. 

Equipment Needed 

Hand truck; cable tags and ties; rubber bands; non-magnetic flatblade and Philips-type screwdrivers; hex and Star-type nut drivers; 

needle-nose and standard pliers; secure-bit drivers; small tweezers; 

wire cutters; anti-static bags, bubble-wrap and other packing 

materials; packing tape; paper bags and sturdy boxes; magnifying 

glass; evidence tape; flashlight; printer paper; CDs and 31⁄2-inch 

diskettes; mobile phone; waterproof pen; labels; personal protective 

equipment. 

 Electronic evidence is fragile and sometimes includes timesensitive data of investigative value that is stored on or 

transmitted by electronic devices.

 Handle electronic evidence carefully because it is fragile. It can 

be easily damaged by heat, cold, shock or magnetic fields. 

 Do not use destructive techniques, such as applying 

fingerprinting chemicals, before you examine the electronic 

evidence.

 Federal and state laws regarding the interception of messages 

exist. State laws vary.

a. Contact an electronic evidence collection expert as soon as 

discovering computer equipment and related components (e.g., 

desktop or laptop computer, printer, disk drive). Never move or 

alter the state of electronic hardware without first talking with an 

electronic evidence collection expert. 

b. Look for proprietary software. Depending on the investigation, 

proprietary software such as database or financial software may 
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have been used by the suspect. Seizure of the original software 

may be critical for the computer forensic analyst’s investigation. 

c. Fulfill the legal requirements necessary for network seizure by 

contacting the appropriate authorities to obtain legal advice and 

warrants, as needed. 

d. To determine legal requirements, follow departmental 

regulations while establishing whether there is probable cause to 

collect:

• Hardware

• Software

• Data

e. Identify whether a warrant is needed or there is an exception to 

the warrant requirement.

f. Determine whether the computer equipment is stand-alone (i.e., 

not attached to a network) or attached to a network. Use these 

methods to locate a network: 

• Interview people at the scene, or informants. 

• Look for multiple computers in the area. 

• Determine whether a printer is in the area.

• Look for cables or connectors linking a computer, card 

scanner, fax machine, etc., to each other, to a phone jack or 

phone, to a printer, etc. 

• When a laptop is present, look for a network cable or a 

USB modem from a cell carrier’s network (Verizon, ATT, 

Sprint) in a USB port. 

• If no cables or USB modem are apparent and multiple 

devices are in the area, consider that a wireless network 

(WiFi) may be present. Work closely with an electronic 

evidence collection expert in this situation. 

g. Establish whether the evidence will be processed on- or off-site 

and make the necessary arrangements. Determine whether 

components or data will be damaged or degraded if the network 

is moved. 

h. Determine whether an outside electronic evidence collection 

expert is needed and available to: 

• Remove the network components or provide instructions 

on how to remove them. 
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• Conduct the network and data investigation or provide 

instructions on how to do so. 

i. Maintain the state of the computer components as found until 

after documentation is complete and after being directed to do 

otherwise.

j. Until there is an electronic evidence collection expert advising, 

do not disturb the network components. Whether the hardware 

was ON or OFF when found, leave it in that state. Never turn 

off power to the hardware (such as a computer, printer, phone) 

without: 

• Being advised to do so by an electronic evidence collection 

expert.

• Following shutdown procedures for that piece of hardware 

(locate and use documentation when needed). 

k. If it is determined that the device is running low on battery 

power, work with the expert to resolve this situation. 

Documentation

a. Document the state of the machine as it was found using 

photography, sketching, and note-taking.

b. Photograph the screen, the back of the computer, and the 

connections to other equipment (e.g., printer, external drive). 

c. Sketch a diagram of the connections between the computer and 

other equipment. 

d. Record information that supports the diagram and photographs, 

including the state of the computer, existing connections, and: 

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial numbers 

e. When preparing a computer for packaging, label the cable with 

the port and evidence numbers before removing the cable from 

the port. 

f. Starting in the upper left corner of the device, assign each port a 

number (e.g., Port 1). Write the port number and evidence 

number on a label or piece of tape and adhere it to the cable. 

g. Do the same for the other end of the cable. If the other end of 

the cable is plugged into the wall, indicate this.
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h. After each cable is removed from a port, label the port with the 

assigned port number. Write the port number on a label or piece 

of tape. After the cable is removed from the port, place the label 

or tape over the port. 

Marking Evidence 

Evidence labeling: Place a label on each component that will be 

collected. Include your initials and identification number, the date 

and time, evidence number, and evidence description. Most often, 

only central processing units (CPUs) and the internal and external 

storage media (such as hard drives, CDs, or DVDs) are collected.

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the numbered placard 

next to the evidence. 

Evidence description: The evidence description includes:

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial number 

When a component is large, such as a monitor, and/or needs to be 

fingerprinted, only attach a tag directly to it unless the lead 

investigator provides the direction to place it in a transport container. 

When practical, wrap each component with anti-static or other 

protective material. Wrap items to protect them from physical 

damage and from loss of data due to magnetic exposure. 

When a component needs to be fingerprinted, do not wrap it. 

Packaging

a. Place the component into the labeled container. 

b. Package items to protect them from damage due to dropping or 

other physical impacts and from loss of data due to magnetic 

exposure: 

c. Use large, plastic bubble-wrap or foam pads as packing material 

when original packing materials are not available.

d. Do not use Styrofoam as it creates static charges.

e. Protect internal storage from data loss (e.g., keep the CPU away 

from magnetic sources such as radios, heated seats, speakers).

f. CPUs and hard drives should be packaged to protect 

fingerprints and transported upright, in a position that prevents 

movement while traveling. 
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g. Monitors should be packaged and transported in a secure 

manner while protecting the screen and fingerprints. 

h. Close the container and completely seal the opening with 

evidence tape. Write your initials and identification number and 

the time and date across the evidence tape seal.

i. When transporting fragile computer components, label the 

outside of the transport container with handling instructions. 

j. When transporting a CPU or hard drive, attach a label indicating:

• THIS END UP

• FRAGILE

• SENSITIVE ELECTRONIC EQUIPMENT

• KEEP AWAY FROM MAGNETIC FIELDS

k. Some data may be lost if battery power ceases while the related 

computer components are in storage. Inform the necessary 

personnel of computer components that rely on battery power 

so that they can be maintained. When seizing laptops, tablet 

computers, and other battery powered devices, make every 

effort to locate and seize the power cables for the device. 

l. Store computer components in a secure area, in a controlled 

climate away from magnetic sources, dust, and other 

contaminants. 

m. Computer components can be damaged when stored in areas 

where temperatures and humidity vary significantly.

Procedure: Non-networked Computers 

Equipment Needed 

Hand truck; cable tags and ties; rubber bands; non-magnetic flatblade and Philips-type screwdrivers; hex and Star-type nut drivers; 

needle-nose and standard pliers; secure-bit drivers; small tweezers; 

wire cutters; anti-static bags, bubble-wrap and other packing 

materials; packing tape; paper bags and sturdy boxes; magnifying 

glass; evidence tape; flashlight; printer paper; CDs and 31⁄2-inch 

diskettes; mobile phone; waterproof pen; labels; personal protective 

equipment. 

 Electronic evidence is fragile and sometimes includes timesensitive data of investigative value that is stored on or 

transmitted by electronic devices.

 Handle electronic evidence carefully because it is fragile. It can 

be easily damaged by heat, cold, shock or magnetic fields. 
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 Do not use destructive techniques, such as applying 

fingerprinting chemicals, before you examine the electronic 

evidence.

 Federal and state laws regarding the interception of messages 

exist. State laws vary.

a. Contact an electronic evidence collection expert as soon as 

discovering computer equipment and related components (e.g., 

desktop or laptop computer, printer, disk drive). Never move or 

alter the state of electronic hardware without first talking with an 

electronic evidence collection expert. 

b. Look for proprietary software. Depending on the investigation, 

proprietary software such as database or financial software may 

have been used by the suspect. Seizure of the original software 

may be critical for the computer forensic analyst’s investigation. 

c. Determine whether the computer equipment is stand-alone (i.e., 

not attached to a network) or attached to a network.

d. Use these methods to locate a network: 

1. Interview people at the scene or informants. 

2. Look for multiple computers in the area. 

3. Determine whether a printer is in the area.

4. Look for cables or connectors linking a computer, card 

scanner, fax machine, etc., to each other, to a phone jack or 

phone, to a printer, etc. 

5. When a laptop is present, look for a network cable or a 

USB modem from a cell carrier’s network (Verizon, ATT, 

Sprint) in a USB port. 

6. If no cables or USB modem are apparent and multiple 

devices are in the area, consider that a wireless network 

(WiFi) may be present. Work closely with an electronic 

evidence collection expert in this situation. 

e. Fulfill the legal requirements necessary for seizure of the 

computer and related hardware and software by contacting the 

appropriate authorities to obtain legal advice and warrants, as 

needed. 

f. To determine legal requirements, follow departmental 

regulations while establishing whether there is probable cause to 

collect:

• Hardware

• Software
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• Data

g. Identify whether a warrant is needed or there is an exception to 

the warrant requirement.

h. Establish whether the evidence will be processed on- or off-site 

and make the necessary arrangements. Determine whether 

components or data will be damaged or degraded if the 

computer is moved. 

i. Determine whether an outside electronic evidence collection 

expert is needed and available to: 

• Remove the computer or provide instructions on how to 

remove it.

• Conduct investigation of the computer and related 

components or provide instructions on how to do so. 

j. Maintain the state of the computer components as found until 

after documentation is complete and after being directed to do 

otherwise.

k. Until there is an electronic evidence collection expert advising, 

do not disturb the network components. Whether the hardware 

was ON or OFF when found, leave it in that state. Never turn 

off power to the hardware (such as a computer, printer, phone) 

without: 

• Being advised to do so by an electronic evidence collection 

expert.

• Following shutdown procedures for that piece of hardware 

(locate and use documentation when needed). 

l. If it is determined that the device is running low on battery 

power, work with the expert to resolve this situation. 

Documentation

a. Document the state of the machine as it was found using 

photography, sketching, and note-taking.

b. Photograph the screen, the back of the computer, and the 

connections to other equipment (e.g., printer, external drive). 

c. Sketch a diagram of the connections between the computer and 

other equipment. 

d. Record information that supports the diagram and photographs, 

including the state of the computer, existing connections, and: 

• Equipment type 

• Equipment location 
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• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial numbers 

e. When preparing a computer for packaging, label the cable(s)

with the port and evidence numbers before removing the 

cable(s) from the port(s). 

• Starting in the upper left corner of the device, assign each 

port a number (e.g., Port 1). Write the port number and 

evidence number on a label or piece of tape and adhere it to 

the cable.

• Do the same for the other end of the cable(s). If the other 

end of the cable is plugged into the wall, indicate this. 

• After each cable is removed from a port, label the port with 

the assigned port number. Write the port number on a label 

or piece of tape. After the cable is removed from the port, 

place the label or tape over the port (see photo, next page). 
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Evidence Marking 

Evidence packaging: Place a label on each component that will be 

collected. Include your initials and identification number, the date 

and time, evidence number, and evidence description. 

Most often, only central processing units (CPUs) and the internal and 

external storage media (such as hard drives, CDs, or DVDs) are 

collected.

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the numbered placard 

next to the evidence. 

Evidence description: The evidence description includes: 

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial number 

Label a container for each component. Include your initials and 

identification number, the date and time, evidence number, and 

evidence description. 
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 When a component is large, such as a monitor, and/or needs to 

be fingerprinted, only attach a tag directly to it unless the lead 

investigator provides the direction to place it in a transport 

container. 

 Whenever possible, use the original packing materials, including 

the fitted padding. Otherwise, use sturdy cardboard containers. 

 When practical, wrap each component with anti-static or other 

protective material. Wrap items to protect them from physical 

damage and from loss of data due to magnetic exposure. When a 

component needs to be fingerprinted, do not wrap it. 

Packaging

a. Place the component into the labeled container. 

b. Package items to protect them from damage due to dropping or 

other physical impacts and from loss of data due to magnetic 

exposure: 

 Use large, plastic bubble-wrap or foam pads as packing material 

when original packing materials are not available.

 Do not use Styrofoam as it creates static charges.

 Protect internal storage from data loss (e.g., keep the CPU away 

from magnetic sources such as radios, heated seats, speakers).

c. CPUs and hard drives should be packaged to protect 

fingerprints and transported upright, in a position that prevents 

movement while traveling. 

d. Monitors should be packaged and transported in a secure 

manner while protecting the screen and fingerprints. 

e. Close the container and completely seal the opening with 

evidence tape. Write your initials and identification number, and 

the time and date across the evidence tape seal.

f. When transporting fragile computer components, label the 

outside of the transport container with handling instructions. 

g. When transporting a CPU or hard drive, attach a label indicating: 

• THIS END UP

• FRAGILE

• SENSITIVE ELECTRONIC EQUIPMENT

• KEEP AWAY FROM MAGNETIC FIELDS

h. Some data may be lost if battery power ceases while the related 

computer components are in storage. Inform the necessary 

personnel of computer components that rely on battery power 
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so that they can be maintained. When seizing laptops, tablet 

computers, and other battery powered devices, make every 

effort to locate and seize the power cables for the device.

i. Store computer components in a secure area that is in a 

controlled climate away from magnetic sources, dust, and other 

contaminants. 

j. Computer components can be damaged when stored in areas 

where temperatures and humidity vary significantly.

Procedure: Other Devices 

Other devices include copy and facsimile (fax) machines, printers, 

scanners, bar coding machines, answering machines, digital cameras, 

GPS devices, etc.

Equipment Needed 

Hand truck; cable tags and ties; rubber bands; non-magnetic flatblade and Philips-type screwdrivers; hex and Star-type nut drivers; 

needle-nose and standard pliers; secure-bit drivers; small tweezers; 

wire cutters; anti-static bags, bubble-wrap and other packing 

materials; packing tape; paper bags and sturdy boxes; magnifying 

glass; evidence tape; flashlight; printer paper; CDs and 31⁄2-inch 

diskettes; mobile phone; waterproof pen; labels; personal protective 

equipment.

 Electronic evidence is fragile and sometimes includes timesensitive data of investigative value that is stored on or 

transmitted by electronic devices.

 Handle electronic evidence as latent evidence because frequently 

it is not readily visible without the aid of other equipment 

and/or software. 

 Do not use destructive techniques, such as applying 

fingerprinting chemicals, before you examine the electronic 

evidence.

 Federal and state laws regarding the interception of messages 

exist. State laws vary.

a. Contact an electronic evidence collection expert as soon as the 

electronic device is discovered. Never move or alter the state of 

electronic hardware without first talking with an electronic 

evidence collection expert.

b. Fulfill the legal requirements necessary for seizure of the device 

by contacting the appropriate authorities to obtain legal advice 

and warrants, as needed.
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c. To determine legal requirements, follow departmental 

regulations while establishing whether there is probable cause to 

collect:

• Hardware

• Software

• Data

d. Identify whether a warrant is needed or there is an exception to 

the warrant requirement.

e. Determine whether the device is stand-alone (i.e., not attached 

to a network) or attached to a network. Use these methods to 

locate a network: 

1. Interview people at the scene or informants. 

2. Look for multiple computers in the area. 

3. Determine whether a printer is in the area.

4. Look for cables or connectors linking a computer, card 

scanner, fax machine, etc., to each other, to a phone jack or 

phone, to a printer, etc.

5. If no cables are apparent and multiple devices are in the 

area, consider that a wireless network may be present. Work 

closely with an electronic evidence collection expert in this 

situation. 

f. Establish whether the evidence will be processed on- or off-site 

and make the necessary arrangements. Determine whether the 

device or data will be damaged or degraded if the device is 

moved. Do not use destructive techniques, such as applying 

fingerprinting chemicals, before you examine the electronic 

evidence.

g. Determine whether an outside electronic evidence collection 

expert is needed and available to:

1. Remove the device or provide instructions on how to 

remove it.

2. Conduct the device and data investigation or provide 

instructions on how to do so. 

h. Maintain the state of the device as found until after 

documentation is complete and after being directed to do 

otherwise.

i. Until there is an electronic evidence collection expert advising, 

do not disturb the network components. Whether the hardware 

was ON or OFF when found, leave it in that state. Never turn 
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off power to the hardware (such as a fax or answering machine) 

without: 

1. Being advised to do so by an electronic evidence collection 

expert.

2. Following shutdown procedures for that piece of hardware 

(locate and use documentation when needed). 

j. If it is determined that the device is running low on battery 

power, work with the expert to resolve this situation. 

Documentation

a. Document the state of the device as it was found using 

photography, sketching, and note-taking.

b. Photograph the screen, the back of the device, and connections 

to other equipment (e.g., camera with its lens cap and power 

cord nearby). 

c. Sketch a diagram of the placement of the device in the area and 

connections to other devices, equipment, and objects. 

d. Record information that supports the diagram and photographs, 

including the state of the device, any existing connections, and: 

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial or other identifying numbers

e. When preparing a device for packaging, label the cable with the 

port and evidence numbers before removing the cable from the 

port. 

f. Starting in the upper left corner of the device, assign each port a 

number (e.g., Port 1). Write the port number and evidence 

number on a label or piece of tape and adhere it to the cable. 

g. Do the same for the other end of the cable. If the other end of 

the cable is plugged into the wall, indicate this.

h. After each cable is removed from a port, label the port with the 

assigned port number. Write the port number on a label or piece 

of tape. After the cable is removed from the port, place the label 

or tape over the port. 

Marking Evidence 

Evidence labeling: Place a label on the device and any 

accompanying components such as battery chargers. Include your 
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initials and identification number, the date and time, evidence 

number, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the numbered placard 

next to the evidence. 

Evidence description: The evidence description includes:

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial number 

Label a container for the device. Include your initials and 

identification number, the date and time, evidence number, and 

evidence description. 

When practical, wrap the device with anti-static or other protective 

material. Wrap items to protect them from physical damage and from 

loss of data due to magnetic exposure. 

When a device needs to be fingerprinted, do not wrap it. 

Packaging

a. Place the device into the labeled container.

b. Package items to protect them from damage due to dropping or 

other physical impacts and from loss of data due to magnetic 

exposure: 

 Use large, plastic bubble-wrap or foam pads as packing 

material when original packing materials are not available.

 Do not use Styrofoam as it creates static charges.

 Protect internal storage from data loss (e.g., keep the CPU 

away from magnetic sources such as radios, heated seats, 

speakers).

c. CPUs and hard drives should be packaged to protect 

fingerprints and transported upright, in a position that prevents 

movement while traveling. 

d. Monitors should be packaged and transported in a secure 

manner while protecting the screen and fingerprints. 

e. After placing the component into a container, close the 

container and completely seal the opening with evidence tape. 

Write your initials and identification number and the time and 

date across the evidence tape seal.
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f. When transporting fragile electronic devices, label the outside of 

the transport container with handling instructions. Attach a label 

indicating: 

• THIS END UP

• FRAGILE

• SENSITIVE ELECTRONIC EQUIPMENT

• KEEP AWAY FROM MAGNETIC FIELDS

g. Some data may be lost if battery power ceases while the related 

device is in storage. Inform the necessary personnel when the 

device relies on battery power so that they can maintain it.

h. Store the device in a secure area, in a controlled climate away 

from magnetic sources, dust, and other contaminants. 

i. Electronic devices, such as cameras, copy, fax and answering 

machines, can be damaged when stored in areas where 

temperatures and humidity vary significantly.

Procedure: Wireless Phones and Pagers10,

11

Wireless phones and pagers, in this context, include cellular phones; 

numeric, alphanumeric and two-way pagers; Personal Digital 

Assistants (PDAs); radio scanners; etc.

 

Equipment Needed 

Faraday Bags, Anti-static bags, bubble-wrap and other packing 

materials; packing tape; paper bags and boxes; evidence tape; 

waterproof pen; labels; personal protective equipment. 

 Phones, pagers, and PDAs are capable of receiving and storing 

information, such as text and voice messages, phone lists, 

memorandums, and caller identification and appointment 

information. 

 Radio scanners can be powered by a cigarette lighter in a vehicle 

and by other common power sources. The scanners can be used 

to monitor phone conversations, detect radar, and capture 

personal Internet or network information from afar. 

 GPS devices may store information on previous locations and 

searches conducted by the user. 

 Federal and state laws regarding the privacy of pagers and 

intercepting messages exist. State laws vary.

 

10 See also Special Considerations When Dealing with Cellular Phones, 

https://www.swgde.org/documents/Current%20Documents . Accessed February 29, 2012 11 See also Best Practices for Mobile Phone Examinations, 

https://www.swgde.org/documents/Current%20Documents. Accessed February 29, 2012.
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a. Fulfill the legal requirements necessary for seizure of the wireless 

phone or pager by contacting the appropriate authorities to 

obtain legal advice and warrants, as needed.

b. To determine legal requirements, follow departmental 

regulations while establishing whether there is probable cause to 

collect:

• Hardware

• Software

• Data

c. Identify whether a warrant is needed or there is an exception to 

the warrant requirement.

d. Maintain the state of the wireless phone or pager as found until 

after documentation is complete and after being directed to do 

otherwise.

e. Whether the device was ON or OFF when found, leave it in that 

state until advised by an electronic evidence collection expert. 

Never turn off a mobile phone or pager without: 

• Being advised to do so by an electronic evidence collection 

expert.

• Following shutdown procedures (locate and use 

documentation when needed). 

f. If it is determined that the wireless phone or pager is running 

low on battery power, work with the expert to resolve this 

situation. 

Documentation

a. Document the state of the wireless phone or pager as it was 

found using photography, sketching, and note-taking.

b. Photograph the screen, the back of the phone or pager, and 

other related equipment (e.g., battery charger regardless of 

whether phone is connected to the charger). 

c. Sketch a diagram of the placement of the device in the area and 

relative to other devices, equipment, and objects. 

d. Record information that supports the diagram and photographs, 

including the state of the wireless phone or pager, existing 

connections, and: 

• Equipment type 

• Equipment location 
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• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial or other identifying numbers 

e. Establish whether the evidence will be processed on- or off-site 

and make the necessary arrangements. Determine whether the 

device or data will be damaged or degraded if it is moved. Do 

not use destructive techniques, such as applying fingerprinting 

chemicals, before you examine the wireless phone or pager 

evidence.

f. Determine whether an outside electronic evidence collection 

expert is needed and available to:

• Examine the wireless phone or pager and related data.

• Provide instructions on how to do so. 

Marking Evidence 

Evidence packaging: When preparing a wireless phone or pager for 

packaging, label a container with your initials and identification 

number, the date and time, evidence number, and evidence 

description. 

Whenever possible, use the original packing materials, including the 

fitted padding. Otherwise, use sturdy cardboard containers. 

Retrieve and store power cables and adapters with recovered phones 

and pagers. It may be necessary to use the power cable or adapter for 

a device while the device is stored.

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the numbered placard 

next to the evidence. 

Evidence description: The evidence description includes:

• Equipment type 

• Equipment location 

• Equipment appearance/condition, including visible damage 

or other characteristics

• Serial or other identifying number 

When practical, wrap the wireless phone or pager with anti-static or 

other protective material. Wrap items to protect them from physical 

damage and from loss of data due to magnetic exposure. 

When a wireless phone or pager needs to be fingerprinted, do not 

wrap it.
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Packaging

a. Place the wireless phone or pager into the labeled container. 

Wireless devices should be packaged in a Faraday bag or in a 

manner that prevents them from receiving incoming 

transmissions. Many devices have the ability to receive 

commands to delete all data or be remotely locked. One method 

of preventing incoming signals, if a Faraday bag is not available, 

is to place the device into “Airplane mode”. 

b. Package items to protect them from damage due to dropping or 

other physical impacts and from loss of data due to magnetic 

exposure: 

 Use large, plastic bubble-wrap or foam pads as packing 

material when original packing materials are not available.

 Do not use Styrofoam as it creates static charges.

 Protect internal storage from data loss (e.g., keep away from 

magnetic sources such as radios, heated seats, speakers).

c. Label the outside of the container with handling instructions. 

d. When transporting electronic evidence, attach a label indicating:

• FRAGILE

• SENSITIVE ELECTRONIC EQUIPMENT

• KEEP AWAY FROM MAGNETIC FIELDS

e. Some data may be lost if battery power ceases while the wireless 

phone or pager is in storage. Inform the necessary personnel of 

wireless phones or pagers that rely on battery power so that they 

can be maintained.

f. Store the wireless phone or pager in a secure area, in a controlled 

climate away from magnetic sources, dust, and other 

contaminants. 

g. Electronic devices can be damaged when stored in areas where 

temperatures and humidity vary significantly.

Fingerprints 

Processing Material for Fingerprints 

The processing of items for fingerprints is done in accordance with 

the goals of the investigation. The prints should be obtained using 

accepted practices for that type of item, taking into consideration the 

weather conditions, substrate, matrix and nature of the case. The 

following procedures are addressed in this section: 

• Procedure: Latent Fingerprints 

Courtesy Ron Smith 

& Associates
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• Procedure: Patent (Visible) Fingerprints 

• Procedure: Chemical Processing

Physical Processes: Latent Fingerprints 

Equipment Needed 

Fingerprinting kit (fingerprint powder, soft bristled brush, single-use 

brushes if chemical or biological contamination is possible, lifting 

tape, 81⁄2" x 11" card stock papers); contact paper; envelopes; 

waterproof pen; evidence tape; ruler; identification labels; protective 

gloves; face protection.

 Prints on evidence are fragile. The slightest amount of handling 

can degrade a print.

 Whenever possible, collect the object on which you find prints. 

 Photograph the powdered print before collecting it. 

 Always use clean gloves when handling evidence.

 Remember: Your prints and DNA may be transmitted by gloves 

when you touch anything, such as when you scratch your nose. 

a. Identify the object that needs to be dusted. 

• Smooth surfaces yield the best latent prints. 

• When looking for latent prints, examine windows, mirrors, 

glasses, door handles, doors, etc. 

b. Position the print powder, brush, and lifting tape within reach of 

the object. 

• Use a powder color that contrasts with the background of 

the item from which the print is being collected. 

• The color of the backing material must contrast with the 

color of the print powder used. 

c. Gently brush the powder onto the object. When available, 

follow manufacturer’s development process instructions. 

1. Pour a small amount of powder on a clean piece of paper 

or jar lid.

2. Lightly dip the brush into the powder. 

3. Tap the brush lightly to cause excess powder to fall off of 

the brush onto the paper. 

4. Carefully and gently brush the object being printed using 

curved strokes that follow the natural lines of the print. 
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Note: Don’t ever blow on the surface since it can 

contaminate the surface with DNA.

Documentation

Endeavor to photograph the developed print. When photographing 

the developed print: 

 Ensure that each photograph shows the scale and identification 

label.

 Always shoot location photographs as well as one-to-one/closeup photographs. 

Evidence Marking 

Evidence packaging: Label a lift card for the print with your initials 

and identification number, the date and time, evidence number, and 

evidence description. 

Record the information on the back of the card holding the lifted 

print. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of print being collected 

• Location of the print 

• Orientation of the print to north or to a prominent nearby 

object; e.g., “Fingerprints found on telephone receiver.” 

• A brief diagram of the location of the print on an object, 

depicted with an "x".

Lifting Fingerprints 

a. Remove a piece of lifting tape.

b. If a pre-made tape lift is being used, open the tape lift and 

remove the protective seal over the sticky part of the tape.

c. If contact paper is being used, remove the protective backing 

that covers the adhesive side of the paper.

d. Press the sticky side of the lifting tape to the developed print. 

Use firm but gentle pressure taking care not to smear the print. 

e. Place the sticky side of the lifting tape onto the card stock. 

Protect the print by pressing the lifting tape to the card stock 

while taking care not to smear the print. 
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Packaging

a. Place the print lift in a container.

b. Multiple print lifts can be placed in the same container. 

c. Submit all print lifts to the laboratory. Do not attempt to 

determine which lifts are suitable for comparison purposes. 

d. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

e. If printing a surface that may contaminate the fingerprint brush 

or powder with physiological fluids or controlled substances, do 

not use them on subsequent items until they have been 

decontaminated. 

f. If possible, collect the item on which the print was found. 

g. If the print is still moist, allow it to dry before collecting it. 

h. When packaging an item with a developed print on it, be sure 

the transport container is made of paper and large enough to 

hold the item without damaging the print. 

i. Place the item with the print, print side up, into the container 

while protecting the print from being touched. Secure the item 

so that the print will not move or be disturbed during transport. 

j. Seal the container with the evidence tape. Label the evidence 

tape.

Procedure: Patent (Visible) Fingerprints 

Equipment Needed 

Photography kit; flashlight and other available light sources; 

containers; waterproof pen; evidence tape; ruler; identification labels; 

protective gloves; face protection

 Prints on evidence are fragile. The smallest amount of handling 

can degrade a print.

 Always photograph the print before collecting it. 

 Always use clean gloves when handling evidence.

 Your prints may be transmitted through your gloves when you 

touch anything, such as when you scratch your nose. 

Documentation

Photograph the visible print. When photographing the print: 

• Ensure that each photograph shows the scale and 

identification label.
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• Always shoot location photographs as well as one-toone/close-up photographs. 

Collection

• If possible, collect the item containing the visible print.

• If the print is still moist, allow it to dry before collecting it. 

Packaging

a. Select a suitable container to transport the print. Make sure it is 

made of paper and is large enough to hold the item without 

damaging the print. 

b. Place the item with the print on it, print side up, into the 

transport container while protecting the print from being 

touched. 

c. Secure the item so that the print will not move or be disturbed 

during transport. 

d. Seal the container with the evidence. Label the evidence tape.

Procedure: Chemical Processing 

 Chemical processing is best performed in a laboratory or 

controlled environment. Chemical processing involves safety 

considerations since the chemicals used may constitute a hazard.

 Chemical processing should only be performed by someone 

trained in the use of the process in the field. 

Some of the chemical processes available are listed in the table below: 

Chemical Reagent Suitable Surfaces

Ninhydrin

(Caution: If ninhydrin is used at 

the crime scene, proper safety 

precautions must be taken. 

Solvents used in the preparation 

of ninhydrin can be flammable 

or deplete oxygen.)

Porous surfaces:

• Paper

• Wood

• Wall board

Cyanoacrylate Ester or Super 

Glue

Non-porous surfaces:

• Glass

• Metal

• Semi-porous surfaces (e.g., 

glossy or coated)

Small particle reagent • Wet surfaces
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Crystal Violet • Sticky side of adhesive tapes

Sudan Black • Plastic baggies

• Coated drinking cups and 

plates

• Food stuff–contaminated 

non- and semi-porous items

• Cyanoacrylate ester–

processed items

Amido Black, Leuco-Crystal 

Violet, DAB, etc. • Blood-contaminated 

impressions

Latent prints can also be enhanced by use of a forensic light source

in combination with the following processing chemicals and 

powders: 

• DFO

• Indanedione 

• Rodamine 6G, RAM, Basic Yellow, etc.

• Redwop, Greenwop

Comparison/Elimination Prints 

Procedure: Inked Prints 

Equipment Needed 

Fingerprinting kit (fingerprint ink pads, fingerprint cards, fingerprint 

card holders); ink remover towels or waterless hand cleaner; 

envelopes; waterproof pen; identification labels; protective gloves 

 Coordinate with the Medical Examiner or Coroner before 

attempting to collect prints from a deceased person. Protect a 

deceased person’s hands and feet by covering them with paper 

bags that are secured at the victim’s wrist and ankle. Never use 

plastic bags to protect these areas of a body.

 Elimination prints can be collected after evidence collection has 

occurred. 

 The preferred method for collecting comparison/elimination 

prints is using ink.

 Finger, palm, hand, toe, heel, feet, and major case prints can be 

inked and rolled. 

 Always use clean gloves when handling evidence. 
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a. Before collecting prints, ensure that the hands are photographed 

to document any trace material or physiological fluid which may 

be present, and collect the material before proceeding.

b. Set up the location where prints will be taken, preferably on a 

flat surface. Ensure that fingerprint supplies are easily accessible 

and ready for use. 

c. Instruct the person to clean their hands before rolling the prints 

if applicable. 

d. Complete as much of the information requested on the print 

card as possible. Critical information includes your name and 

identification number, the name of the fingerprinted person, the 

date and time, and which hand was printed. 

e. Insert the print card into a card holder. 

f. Position the person being printed so that the hands are easily 

accessible to you without your firearm side being exposed, if you 

are wearing a firearm.

g. Just before rolling prints, put on clean gloves to ensure that you 

have eliminated the possibility of transferring your prints to the 

card.

h. The person being printed should stand facing the print card. 

You can stand, being sure to safeguard your firearm if you are 

wearing one, to the side of the hand being printed or in front of 

the person. 

i. Hold the person’s thumb tip and wrist gently, and press the 

thumb onto the ink strip. Roll the thumb on the ink from the 

edge of the nail on one side to the edge of the nail on the other 

side.

j. Be sure to ink the tip of the thumb (or finger) well so that the 

developed print is as clear and complete as possible. 

k. Gently and with steady force, press the inked thumb to the card 

and roll it from the edge of the nail on one side to the edge of 

the nail on the other side. 

l. Repeat the inking and rolling process for all fingers on one hand, 

starting with the index finger. Print the fingers in this order: 

index, middle, ring, and little.

m. When a print is smudged or otherwise defective on the card, 

take another print on a new card for that finger only. Label the 

card so that it clearly corresponds with the card containing the 

smudged print. Never discard a print card; do not cover the 

print with a fingerprint tab designed for this purpose. 
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n. When print impressions are not clear due to a skin condition or 

other circumstance, write “Best print possible due to (reason)” 

in the space nearest the print on the card.

o. When a finger is injured or missing, note the condition in the 

space for that finger.

p. When necessary, obtain a new card for the prints from the other 

hand. When using a ten print card, use the space provided for 

the other hand. 

q. Package to protect the completed card.

Procedure: Flexible Lifter 

Equipment Needed 

Fingerprinting kit (fingerprint powder, soft bristled brush); flexible 

plastic lifters (e.g., Handiprint©, Kinderprint©); fingertip moistener; 

waterless hand cleaner; envelopes; waterproof pen; protective gloves.

 Coordinate with the Medical Examiner or Coroner before 

attempting to collect prints.

 Protect a dead victim’s hands and feet by covering them with 

paper bags that are secured at the victim’s wrist and ankle.

 Elimination prints can be collected after evidence collection has 

occurred. 

 Finger, palm, hand, toe, heel, and feet prints, and major case 

prints can also be developed using this method. 

 Always use clean gloves when handling evidence.

a. Prepare to take the prints by setting up the location where prints 

will be taken and instructing the person to clean their hands if 

they are very soiled.

b. Before collecting prints, ensure that the hands are photographed 

to document any trace material or physiological fluid which may 

be present, and collect the material before proceeding.

c. Complete as much of the information requested on the print 

card as possible. Critical information includes your name and 

identification number, the name of the fingerprinted person, the 

date and time, and which hand was printed. 

d. Avoid touching the lifter in the area where the person’s prints 

will be developed. 

e. Cut a sheet of flexible plastic lifter into 11⁄2" x 8" strips to record 

fingers.
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f. Rub a small amount of fingertip moistener onto the subject’s 

fingers. Any excess may be wiped off with a paper towel. 

g. Lightly dust palm side of hand with black fingerprint powder. 

h. Separate release paper from flexible plastic lifter. 

i. Spread fingers and press hand on adhesive side of lifter. 

j. Lift hand. Press the lifter along the length of each finger, and 

around the sides of the fingertips. 

k. Press around thumb as much as possible. 

l. Larger pieces of lifter can be used to capture palm detail.

m. Prepare a backing material, such as clear acetate, and cover the 

adhesive side of the lifter with the acetate. Write identifying 

information on the back of the lifter. 

n. Trim and add to a tenprint fingerprint card, if applicable. 

o. When print impressions are not clear due to a skin condition or 

other circumstance, write “Best print possible due to (reason).” 

in the space nearest the print on the card.

p. When a thumb or finger is injured or missing, note the condition 

of the thumb or finger in the space for the print. 

q. Repeat the rolling and dusting process to collect the thumb and 

fingerprints from the person’s other hand. 

r. Place the print cards in the container.

Tool Mark Evidence 

A tool mark is any impression, scratch, gouge, cut, or abrasion made 

when a tool is brought into contact with an item, leaving an 

impression of the tool. In some cases, tool mark identification may 

link a person to the tool used in the commission of a crime. 

Equipment Needed 

Casting kit (e.g., DuplicastTM, MikrosilTM, silicone-type sealant); 

mixing pad; stirring stick; flashlight and other available light sources; 

paper envelopes and boxes; waterproof pen; measuring tape/ruler; 

identification labels; protective gloves; face protection.

 Photograph the impression before casting it. 

 When making a cast, be prepared to act quickly and 

methodically. Time is often a critical factor in successfully 

making a cast.

 Always use clean gloves when handling evidence.
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Documentation

Photograph the impression. When photographing the object: 

• Include a scale and an identification label.

• Take one or more location photographs that show the 

object where it was found. 

• Show the relationship of the object to other evidence in the 

photograph. 

Evidence Marking 

Evidence labeling: Label a container for the object with your initials 

and identification number, the date and time, evidence number, 

location, and evidence description. 

Evidence number: Each piece of evidence must have a unique 

number. This number should correspond to the placard next to the 

evidence.

Evidence description: The evidence description includes:

• Type of item being cast

• Location of the item being cast

• Orientation of the item being cast to north, to a feature of 

the object with the impression on it, or to a nearby object. 

Casting the Evidence

a. Label the container just before collecting an object, and seal the 

container immediately after collection. 

b. Clean out loose material from the impression, when possible, 

without disturbing the impression. Never discard a cast, 

regardless of condition when removed from the impression. Be 

sure to save and submit all casts to the laboratory. 

Packaging

a. Close the container and seal the entire opening with evidence 

tape. Write your initials and identification number, and the date 

and time across the evidence tape seal.

b. Make sure that the container is also labeled with a description of 

the item cast, your initials and identification number, the date 

and time, location and, when possible, the evidence number. 

c. If the item with the tool mark is collected, it should be packaged 

to prevent any additional marks, impressions or other damage. 

Never place the suspected item into the impression. 

Summary: Evidence at crime scenes that is in the process of 

documentation, collection, preservation, or packaging should be 
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handled with attention to scene integrity and protection from 

contamination or deleterious change. During the processing of the 

scene, and following documentation, evidence should be 

appropriately packaged, labeled, and maintained in a secure, 

temporary manner until final packaging and submission to a secured 

evidence storage facility or the crime laboratory. 
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Completing and Recording 

the Crime Scene 

Investigation 

1. Establish Crime Scene Debriefing 

Team

Principle: The crime scene debriefing enables law enforcement 

personnel and other responders to share information regarding 

particular scene findings prior to releasing the scene. It provides an 

opportunity for input regarding follow-up investigation, special 

requests for assistance, and the establishment of post-scene 

responsibilities.

Policy: Law enforcement personnel and other responders shall 

participate in or initiate a crime scene debriefing to ensure the crime 

scene investigation is complete and to verify post-scene 

responsibilities.

Procedure: The investigator(s) in charge of the crime scene 

should establish a crime scene debriefing team. When participating in 

a scene debriefing, law enforcement personnel and other responders 

should: 

a. Establish a crime scene debriefing team, which includes the 

investigator(s) in charge of the crime scene, other investigators 

and evidence collection personnel (e.g., photographers, 

evidence technicians, latent print personnel, specialized 

personnel, and initial responding officer(s) if still present).

b. Determine what evidence was collected.

c. Discuss preliminary scene findings with team members. 

d. Discuss potential technical forensic testing and the sequence of 

tests to be performed. 

e. Initiate any action(s) identified in discussion that are required to 

complete the crime scene investigation.

f. Brief person(s) in charge upon completion of assigned crime 

scene tasks.

g. Establish post-scene responsibilities for law enforcement 

personnel and other responders. 

D.
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Summary: The crime scene debriefing is the best opportunity for 

law enforcement personnel and other responders to ensure that the 

crime scene investigation is complete.

2. Perform Final Survey of the Crime 

Scene

Principle: Final survey of the crime scene ensures that evidence 

has been collected and the scene has been processed prior to release. 

In addition, a systematic review of the scene ensures that evidence, 

equipment, or materials generated by the investigation are not 

inadvertently left behind and any dangerous materials or conditions 

have been reported and addressed. 

Policy: The investigator(s) in charge shall direct a walk-through at 

the conclusion of the scene investigation and ensure that the scene 

investigation is complete.

Procedure: The investigator(s) in charge should ensure that: 

a. Each area identified as part of the crime scene is visually 

inspected. 

b. All evidence collected at the scene is accounted for.

c. All equipment and materials generated by the investigation are 

removed. 

d. Any dangerous materials or conditions are reported and 

addressed. 

e. The crime scene is released in accordance with jurisdictional 

requirements. 

f. Consider taking photographs depicting the condition of the 

scene at the time.

Summary: Conducting a scene walk-through ensures that all 

evidence has been collected, that materials are not inadvertently left 

behind, and that any dangerous materials or conditions have been 

reported and addressed. 

3.Documentation of the Crime Scene

Principle: Compiling reports and other documentation pertaining 

to the crime scene investigation into a “case file” provides a record of 
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the actions taken and evidence collected at the scene. This 

documentation allows for independent review of the work 

conducted. 

Policy: The investigator(s) in charge shall ensure that reports and 

other documentation pertaining to the crime scene investigation are 

compiled. 

Procedure: The investigator(s) in charge should obtain the 

following for the crime scene case file:

a. Initial responding officer(s’) documentation. 

b. Emergency medical personnel documents.

c. Entry/exit documentation. 

d. Photographs/videos. 

e. Crime scene sketches/diagrams.

f. Evidence documentation. 

g. Other responders’ documentation. 

h. Record of consent form or search warrant. 

i. Reports such as forensic/technical reports, when they become 

available.

Note: The above list is limited to crime scene documentation. This 

should not be considered a comprehensive list of the documents 

involved in an investigative case file.

Summary: This procedure will ensure that reports and other 

documentation pertaining to the crime scene investigation are 

compiled into a case file by the investigator(s) in charge of the crime 

scene and allow for independent review of the work conducted. 

4. Acknowledge Specialized Crime Scene 

Circumstances

Principle: While all crime scene investigations pose their 

individual complexities, some situations may involve atypical crime 

scene locations or requirements for which Law enforcement 

personnel and other responders should be aware. 

Policy: Law enforcement personnel and other responders shall 

vigilantly prepare for crime scene investigations under specialized 

crime scene circumstances.
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Procedure: Crime scene investigators should adjust their 

approach to an investigation to warrant specific needs of the 

investigation which includes:

• Crime Scenes in correctional and custodial facilities

• Crime scenes in which the safety of the crime scene 

investigators must be considered in the approach to the 

time spent at the scene

Crime Scene Investigation in Correctional and 

Custodial Facilities 

Investigations conducted in correctional and custodial facilities 

require significant awareness on the part of the crime scene 

investigator. Using the appropriate search methodology is key to a 

thorough investigation. Additional guidance is provided if the focus 

of the search is a place of confinement, e.g., a cell. 

a. Use a search method appropriate for the size of the area of 

confinement. 

b. Consider that evidence could exist or be placed at higher levels 

than is typical in a crime scene. Make sure you look up.

c. Consider that some evidence may be deliberately covered up. 

Move layers of material, looking for potential evidence 

underneath. 

d. Move material aside and search. In cells, this includes: 

• bedding material 

• bedding frames 

• lamps

• air grates

• plumbing and chases

• cell bar attachments, such as to walls

• trash receptacles

e. Carefully examine materials encountered. Even an item such as a 

pen can be modified to be a weapon. Assume nothing is as it 

first appears.

f. Have custodial staff advise if anything appears different or out 

of place for their facility.
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Time-Limited Crime Scene Investigation 

In some instances, deteriorating security or environmental conditions 

limit the amount of time available for the investigation of the crime 

scene. While these time limits will not allow for a thorough crime 

scene investigation to be conducted, the following procedure will 

maximize the use of the limited time onsite. 

In such circumstances, preparation prior to staging or entry into the 

crime scene area is paramount. This could include a site survey (e.g., 

in-person, photographic, photogrammetric or videographic) prior to 

the team’s arrival at the scene or conducting extensive interviews of 

any witnesses from the area. 

Elements of this preparation and execution are designed to: 

a. Determine the time available to remain at the crime scene based 

upon best knowledge of time-limiting factors.

b. Determine the most critical objective of being on the site of the 

investigation; e.g., removal of a deceased body, identification of 

suspect, collection of explosive residue, etc.

c. Determine the equipment needed to fulfill the objective. Prepackage from established crime scene collection kits a ready-kit 

for this specific event. 

d. Determine any specialized personnel that may be needed onscene for this investigation. 

e. Develop a documentation and collection plan to include: 

• Type and nature of documentation expected 

• Priority of evidence collection

• Responsibility for onsite collection 

• Responsibility for evidence custody 

Summary: Crime scene investigation in specialized circumstances 

requires the application of techniques that have been adapted for 

unusual conditions. 
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Crime Scene Equipment 

1. Initial Responding Officer(s)

Essential* 

• Consent/search forms. 

• Crime scene barricade tape. 

• First-aid kit.

• Flares.

• Flashlight and extra batteries. 

• Paper bags.

• Personal protective equipment (PPE)

* These items should be in police vehicles or readily available to 

initial responding officer(s). 

Optional

• Audiotape recorder 

• Camera with flash and extra film, if not digital camera

• Chalk 

• Directional marker/compass

• Disinfectant (such as a 10% bleach solution) 

• Maps

• Plastic bags

• Pocket knife

• Reflective vest

• Tape measure

• Tarps to protect evidence from the weather

• Traffic cones

• Waterless hand wash (towelette with germicide)

• Wireless phone 

E.
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2.Crime Scene Investigator/Evidence 

Technician

Essential* 

• Bindle paper 

• Biohazard bags 

• Body fluid collection kit 

• Camera with flash and tripod; extra film, if not digital; extra 

flash memory cards, if digital

• Casting materials

• Consent/search forms 

• Crime scene barricade tape

• Cutting instruments (knives, box cutter, scalpel, scissors, 

etc.)

• Directional marker/compass

• Disinfectant (such as a 10% bleach solution) 

• Evidence collection containers including rigid containers 

for firearms and ammunition boxes, pie boxes with sheet 

cotton for document recovery; manila folders 

• Evidence identifiers (numbers, placards)_ 

• Evidence seals/tape

• First-aid kit

• Flashlight and extra batteries

• High-intensity lights

• Latent print kit

• Magnifying glass

• Measuring devices

• Permanent markers

• Personal protective equipment (PPE)

• Photographic scale (ruler)

• Presumptive blood test supplies 

• Sketch paper

• Tool kit

• Tweezers/forceps

• Window screen fabric in rolls or sheets 

* These items should be in vehicles or readily available to Crime 

Scene Investigator/Evidence Technician.
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Optional

• Audiotape recorder 

• Bloodstain pattern examination kit 

• Business cards

• Chalk 

• Chemical enhancement supplies

• Compass 

• Entomology (insect) collection kit 

• Extension cords 

• Flares

• Forensic light source (alternate light source, UV lamp/laser, 

goggles)

• Generator

• Gunshot residue kit 

• Laser trajectory kit

• Maps

• Marking paint/snow wax 

• Metal detector

• Mirror

• Phone listing (important numbers) 

• Privacy screens

• Protrusion rod set 

• Reflective vest

• Refrigeration or cooling unit 

• Respirators with filters

• Roll of string. 

• Rubber bands. 

• Sexual assault evidence collection kit (victim and suspect)

• Shoe print lifting equipment 

• Templates (scene and human)

• Thermometer

• Traffic cones

• Trajectory rods

• Video recorder 

• Wireless phone 
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3. Evidence Collection Kits (Examples)

Blood Collection

• Bindle 

• Coin envelopes 

• Disposable scalpels

• Distilled water or single use sterile water droppers

• Evidence identifiers

• Drying box 

• Latex gloves

• Photographic ruler (ABFO scales) 

• Presumptive chemicals

• Swabs

Fingerprint

• Adhesive and gelatin lifting materials

• Brushes

• Chemical enhancement supplies

• Cyanoacrylate (super glue) wand/ packets

• Fingerprint ink pads, cards and card holders for exemplar 

collection

• Flashlight

• Forensic light source 

• Lift cards, including 8 1⁄2” x 11” card stock

• Lift tape

• Measurement scales

• Powders 

Bloodstain Pattern Documentation

• ABFO scales

• Calculator

• Laser pointer 

• Permanent markers

• Protractor

• String

• Tape
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Electronic and Digital Evidence Recovery

• Anti-static bags

• Bubble-wrap and other packing materials

• Cable tags and ties 

• CDs and 31⁄2-inch diskettes 

• Faraday Bags 

• Hand truck 

• Nut drivers, hex and star-type 

• Pliers: needle-nose and standard 

• Rubber bands 

• Magnifying glass

• Printer paper 

• Secure-bit drivers 

• Screwdrivers, non-magnetic flat-blade and Philips-type

• Tweezers, small non-magnetic

• Wire cutters

Excavation and Evidence Recovery

• Cones/markers

• Evidence identifiers

• Hand tools (hammer, chisel/screwdriver, forceps, hand 

saw, box cutter, drywall saw, etc.)

• Metal detectors 

• Paintbrushes 

• Shovels/trowels 

• Sifting screens

• String

• Weights

• Wooden/metal stakes 

Impressions – footwear, tire tracks and tool 

mark

• Bowls/mixing containers 

• Boxes

• Casting Kit (e.g. Duplicast©, Mikrosil© or 

polyvinylsiloxane (PVS) materials, silicone-type sealant)

• Dental stone

• Evidence identifiers
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• Material for forms

• Measurement scales

• Permanent markers

• Snow print wax 

• Stirring sticks

• Water

Pattern Print Lifter

• Chemical enhancement supplies

• Electrostatic dust lifter

• Gel lifter

• Wide format lift tape

Trace Evidence Collection 

• Acetate sheet protectors or clear secondary liners

• Bindle paper or weigh paper for bindles 

• Butcher paper 

• Clear packing/sealing tape 2 1⁄2- to 4 inches wide 

• Cotton-tipped swab 

• Flashlight (oblique lighting) 

• Forceps/tweezers (disposable or clean smooth tipped) 

• Glass jars, bottles, vials with air-tight, screw-on lids 

• Metal friction lid cans with fitting lids

• Slides and slide mailers

• Trace evidence vacuum with disposable collection filters

• Transfer pipettes (glass or plastic)
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Appendices 

Appendix A Glossary

Appendix B Reference List

Appendix C Diagrams
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Appendix A. Glossary 

The definitions below apply to terms as used in this document. 

ABFO scales: (American Board of Forensic Odontology scales). An 

L-shaped piece of plastic used in photography that is marked with 

circles, black and white bars, and 18-percent gray bars to assist in 

distortion compensation and provide exposure determination. For 

measurement, the plastic piece is marked in millimeters. 

Alternate light source (ALS): Equipment used to produce visible 

and invisible light at various wavelengths to enhance or visualize 

potential items of evidence (fluids, fingerprints, clothing fibers, etc.). 

Argon ion laser: The first generation of lasers used for detection of 

latent fingerprints. Expensive and non-portable, they have been 

supplanted by the introduction of solid-state and semi-conductor 

lasers. 

Bindle paper: Clean paper that is folded to contain trace evidence, 

sometimes included as part of the packaging for collecting trace 

evidence. 

Biohazard bag: A container for materials that have been exposed to 

blood or other biological fluids. 

Biological fluids: Fluids that have human or animal origin, most 

commonly encountered at crime scenes (e.g., blood, mucus, 

perspiration, saliva, semen, vaginal fluid, urine). 

Biological weapon: Biological agents used to threaten human life 

(e.g., anthrax, smallpox, or any infectious disease). 

Bloodborne pathogen: Infectious, disease-causing microorganisms 

that may be found or transported in biological fluids. 

Boundaries: The perimeter or border surrounding potential physical 

evidence related to the crime. 

Case file: The collection of documents comprising information 

concerning a particular investigation. (This collection may be kept in 

case jackets, file folders, ring binders, boxes, file drawers, file 

cabinets, or rooms. Sub-files are often used within case files to 

segregate and group interviews, media coverage, laboratory requests 

and reports, evidence documentation, photographs, videotapes, 

audiotapes, and other documents.) 

Case identifiers: The alphabetic and/or numeric characters assigned 

to identify a particular case. 

Chase: A space in a wall or floor for pipes or ducts. 
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Chain of custody: A process used to maintain and document the 

chronological history of the evidence. (Documents should include 

name or initials of the individual collecting the evidence, each person 

or entity subsequently having custody of it, dates the items were 

collected or transferred, agency and case number, victim’s or 

suspect’s name, and a brief description of the item.) 

Chemical enhancement: The use of chemicals that react with 

specific types of evidence (e.g., blood, semen, lead, fingerprints) in 

order to aid in the detection and/or documentation of evidence that 

may be difficult to see. 

Chemical threat: Compounds that may pose bodily harm if 

touched, ingested, inhaled, or ignited. These compounds may be 

encountered at a clandestine laboratory, or through a homemade 

bomb or tankard leakage (e.g., ether, alcohol, nitroglycerin, 

ammonium sulfate, red phosphorus, cleaning supplies, gasoline, or 

unlabeled chemicals). 

Clean/sanitize: The process of removing biological and/or 

chemical contaminants from tools and/or equipment (e.g., using a 

mixture of 10-percent household bleach and water). 

Collect/collection: The process of detecting, documenting, or 

retaining physical evidence. 

Comparison samples: A generic term used to describe physical 

material/ evidence discovered at crime scenes that may be compared 

with samples from persons, tools, and physical locations. 

Comparison samples may be from either an unknown/questioned or 

a known source. 

Samples whose source is unknown/questioned are of three basic 

types: 

1. Recovered crime scene samples whose source is in question 

(e.g., evidence left by suspects, victims). 

2. Questioned evidence that may have been transferred to an 

offender during the commission of the crime and taken 

away by him or her. Such questioned evidence can be 

compared with evidence of a known source and can 

thereby be associated/linked to a person/ vehicle/tool of a 

crime. 

3. Evidence of an unknown/questioned source recovered 

from several crime scenes may also be used to associate 

multiple offenses that were committed by the same person 

and/or with the same tool or weapon. 

Samples whose source is known are of three basic types: 
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1. A standard/reference sample is material of a 

verifiable/documented source which, when compared with 

evidence of an unknown source, shows an association or 

linkage between an offender, crime scene, and/or victim 

(e.g., a carpet cutting taken from a location suspected as the 

point of transfer for comparison with the fibers recovered 

from the suspect’s shoes, a sample of paint removed from a 

suspect’s vehicle to be compared with paint found on a 

victim’s vehicle following an accident, or a sample of the 

suspect’s and/or victim’s blood submitted for comparison 

with a bloodstained shirt recovered as evidence). 

2. A control/blank sample is material of a known source that 

presumably was uncontaminated during the commission of 

the crime (e.g., a sample to be used in laboratory testing to 

ensure that the surface on which the sample is deposited 

does not interfere with testing. For example, when a 

bloodstain is collected from a carpet, a segment of 

unstained carpet must be collected for use as a blank or 

elimination sample). 

3. An elimination sample is one of known source taken from a 

person who had lawful access to the scene (e.g., fingerprints 

from occupants, tire tread impressions from police vehicles, 

footwear impressions from emergency medical personnel) 

to be used for comparison with evidence of the same type. 

Contamination: The unwanted transfer of material from another 

source to a piece of physical evidence. 

Control/blank sample: See comparison samples. 

Cross-contamination: The unwanted transfer of material between 

two or more sources of physical evidence. 

Documentation: Written notes, audio/videotapes, printed forms, 

sketches and/or photographs that form a detailed record of the 

scene, evidence recovered, and actions taken during the search of the 

crime scene. 

Drying box: A box intended to quickly dry multiple swabs with the 

aid of a fan blowing air through a chamber in which multiple swabs 

can be held apart from one another. 

Dying declaration: Statements made by a person who believes he or 

she is about to die, concerning the cause or circumstance surrounding 

his or her impending death. 

Electrostatic dust lifter: A device that operates by charging a plastic 

film, placed over the dust print, which creates electrostatic adhesions 

and draws the film onto the surface bearing the print. The dust 
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particles are attracted to the film because of this charge and adhere to 

it. 

Elimination sample: See comparison samples. 

Evidence identifiers: Tape, labels, containers, and string tags used 

to identify the evidence, the person collecting the evidence, the date 

the evidence was gathered, basic criminal offense information, and a 

brief description of the pertinent evidence. 

Exemplars: A known sample of evidence created at the request of 

an investigator used for comparison to an unknown sample. 

First responder(s): The initial responding law enforcement 

officer(s) and/or other public safety official(s) or service provider(s) 

arriving at the scene prior to the arrival of the investigator(s) in 

charge. 

Faraday bag: Specialty collection bags for electronic parts with 

lining to protect the contents from electromagnetic forces. 

Fluorescent powders: Powder intended to reveal latent prints which 

contain fluorescent chemicals that reveal itself under a forensic light 

source. 

Forensic light source: see Alternate light source (ALS). 

Impression evidence: Objects or materials that have retained the 

characteristics of other objects that have been physically pressed 

against them. 

Infrared photography: A photographic process of recording images 

by using light from the infrared (IR) spectrum only, generally 700 to 

900 nanometers.

Initial responding officer(s): The first law enforcement officer(s) 

to arrive at the scene. 

Investigator(s) in charge: The official(s) responsible for the crime 

scene investigation. 

Known: See comparison samples. 

Latent print: A print impression not readily visible, made by contact 

of the hands or feet with a surface resulting in the transfer of 

materials from the skin to that surface. 

Long-wave ultraviolet (UV) lamp: An ultraviolet light source that 

operates between 300-400 nanometers; useful for quickly scanning 

and documenting crime scenes when used in tandem with a UVsensitive camera. 

Major case prints: The recording of all friction ridge detail on the 

hands. This includes the fingers, fingertips, finger joints and edges of 
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the fingers as well as the entire palm. Also known as “complete 

friction ridge exemplars.” 

Matrix: The substance that is deposited or removed by the friction 

ridge skin when making an impression. 

Measurement scale: An object showing standard units of length 

(e.g., ruler) used in photographic documentation of an item of 

evidence. 

Multiple scenes: Two or more physical locations of evidence 

associated with a crime (e.g., in a crime of personal violence, evidence 

may be found at the location of the assault and also on the person 

and clothing of the victim/assailant, the victim’s/assailant’s vehicle, 

and locations the victim/assailant frequents and resides). 

Nonporous container: Packaging through which liquids or vapors 

cannot pass (e.g., glass jars or metal cans). 

Other responders: Individuals who are involved in an aspect of the 

crime scene, such as perimeter security, traffic control, media 

management, scene processing, and technical support, as well as 

prosecutors, medical personnel, medical examiners, coroners, 

forensic examiners, evidence technicians, and fire and rescue officers. 

Outsole: The portion of footwear that is in direct contact with the 

ground. 

Personal protective equipment (PPE): Articles such as disposable 

gloves, masks, and eye protection that are utilized to provide a barrier 

to keep biological or chemical hazards from contacting the skin, eyes, 

and mucous membranes and to avoid contamination of the crime 

scene. 

Porous container: Packaging through which liquids or vapors may 

pass (e.g., paper bags, cloth bags). 

Porous surface: any surface that has tiny openings that absorbs 

liquids or allows them to pass through (e.g., furniture fabric, canvas, 

wood, wall board) 

Presumptive test: A non-confirmatory test used to screen for the 

presence of a substance. 

Projectile trajectory analysis: The method for determining the path 

of a high-speed object through space (e.g., a bullet emanating from a 

firearm). 

Radiological threat: The pending exposure to radiation energy. 

(This energy can be produced by shortwave X-rays or through 

unstable isotopes.) 
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Single-use equipment: Items that will be used only once to collect 

evidence, such as biological samples, then discarded to minimize 

contamination (e.g., tweezers, scalpel blades, droppers). 

Secondary Liner: Secondary liner is a clear polyester sheet or roll 

used in industry for stickers, seals, and other adhesive-containing 

materials to protect the adhesive until ready for use. 

Standard/reference sample: See comparison samples. 

Substrate: The surface upon which a friction ridge impression is 

deposited. 

Team members: Individuals who are called to the scene to assist in 

investigation or processing of the scene (e.g., scientific personnel 

from the crime laboratory or medical examiner’s office, other 

forensic specialists, photographers, mass disaster specialists, experts 

in the identification of human remains, arson and explosives 

investigators, clandestine drug laboratory investigators, as well as 

other experts). 

Trace evidence: Physical evidence that results from the transfer of 

small quantities of materials (e.g., hair, textile fibers, paint chips, glass 

fragments, gunshot residue particles). 

Transient evidence: Evidence which by its very nature or the 

conditions at the scene will lose its evidentiary value if not preserved 

and protected (e.g., blood in the rain). 

Ultraviolet photography: A photographic process of recording 

images by using light from the ultraviolet (UV) spectrum only, 

generally less than 400 nanometers. 

Unknown/questioned: See comparison samples. 

Walk-through: An assessment conducted by carefully walking 

through the scene to evaluate the situation, recognize potential 

evidence, and determine resources required. Also, a final survey 

conducted to ensure the scene has been effectively and completely 

processed. 
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Appendix B. Reference List

Bevel, T. and R. Gardner. Bloodstain Pattern Analysis. Boca Raton, 

Florida: CRC Press, Inc., 1997.

Bodziak, W.J. Footwear Impressions Evidence. New York: Elsevier

Science Publishing Co., 1990.

Crime Scene and Evidence Collection Handbook. Washington, D.C.: Bureau 

of Alcohol, Tobacco and Firearms, 1999. 

Colorado Evidence Collection Protocol, Colorado Coalition Against Sexual 

Assault, Colorado Bureau of Investigation, 2000. 

Crime Scene Awareness and Investigation, Vienna, Austria: United Nations 

Office on Drugs and Crime, 2011.

Crime Scene Investigation Guidelines, Mendota Heights, MN: 

International Association for Identification, 2010. 

Crime Scene Measurement, 

https://www.criminology.fsu.edu/faculty/nute/CSmeasurement.htm

l, (accessed April 13, 2012).

DeForest, P.R., R.E. Gaensslen, and H.C. Lee. Forensic Science: An

Introduction to Criminalistics. New York: McGraw-Hill, Inc., 1983.

FBI Handbook of Forensic Science, Collection, Identification and Shipping 

Index (with modifications). Washington, D.C.: Federal Bureau of 

Investigation, 1992.

Fisher, B.A.J. Techniques of Crime Scene Investigation, 5th Edition. Boca 

Raton, Florida: CRC Press Inc., 1993.

Forensic and Crime Scene Tool Set, NLECTC – Small, Rural, Tribal and 

Border Regional Center, National Institute of Justice, Office of 

Justice Programs, US Department of Justice, 2010.

Fox, R. H. and C. L. Cunningham. Crime Scene Search and Physical 

Evidence Handbook. Washington, D.C.: U.S. Department of Justice, 

National Institute of Justice, 1973.

Geberth, Vernon, J. Practical Homicide Investigation Checklist and Field

Guide. New York: CRC Press, 1996.

Guide for the Collection of Footwear and Tire Impressions in the 

Field. Journal of Forensic Identification 770 / 55 (6), 2005.

Handbook of Physical Evidence. Miami, Florida: Metro-Dade Police

Department, 1996.

Kirk, P.L. Crime Investigation, 2nd Edition. New York: John Wiley & 

Sons, 1974.
CRIME SCENE INVESTIGATION A Guide for Law Enforcement 

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NIST/NIJ Technical Working Group on Biological Evidence 

Preservation: Best Practice Handbook 

www.cstl.nist.gov/strbase/pub_pres/NIJ2012-Kline-TWGBEP.pdf

Physical Evidence Handbook, 8th Edition. Madison, Wisconsin: 

Wisconsin Department of Justice, 2009.

Physical Evidence Manual, State of Oregon, Forensic Services 

Division, 2008 

http://egov.oregon.gov/OSP/FORENSICS/LawEnfResources.sht

ml, (accessed April 14, 2012).

Rini, Gary A. Crime Scene Search and Physical Evidence Management: 

Student Training Manual. Elkhorn, Nebraska: The American Institute 

for Police Science, 1998.

Saferstein, R. Criminalistics: An Introduction to Forensic Science, 6th Edition. 

Englewood Cliffs, N.J.: Prentice-Hall, 1998.

Saferstein, R. Forensic Science Handbook, Volumes I, II, III. Englewood

Cliffs, N.J.: Prentice-Hall, 1982/1988/1993.

Suggested Guidelines for Establishing Evidence Response Teams. Washington, 

D.C.: Federal Bureau of Investigation Laboratory. 

Related References

Dirkmaat, D., E. Chapman, M. Kenyhercz, and L. Cabo. Enhancing 

Scene Processing Protocols to Improve Victim Identification and Field Detection 

of Human Remains in Mass Fatality Scenes, Washington, D.C.: U.S. 

Department of Justice, National Institute of Justice, 2012. 
CRIME SCENE INVESTIGATION A Guide for Law Enforcement 

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Appendix C. Diagrams

C-1 Overview Sketch 
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C-2 Perspective Sketch 

C-3 Projection Sketch 

 Cases

 Cases

 Possible
CRIME SCENE INVESTIGATION A Guide for Law Enforcement 

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C-4 Creating a Druggist Bindle 

 

C-5 Creating an Envelope Bindle 

All diagrams courtesy of Wayne Moorehead.
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C-6 Crime Scene Entry Log