# Patent

## Title
Compositions and methods of their use for improving the condition and appearance of skin

## Abstract

The present invention relates to compositions and methods for treating, preventing and improving the condition and aesthetic appearance of skin, particularly, treating, preventing, ameliorating, reducing and/or eliminating fine lines and/or wrinkles of skin, where the compositions include natural plant constituents which increase expression levels genes associated with the dermatological signs of aging. The compositions of the invention are topically applied to the skin, or are delivered by directed means to a site in need thereof, once daily in an amount effective in improving the condition and aesthetic appearance of skin.

## Background
FIELD OF THE INVENTION
This invention generally relates to cosmetic compositions and their use, and more particularly to cosmetic compositions and to their use in improving the condition and appearance of skin.
BACKGROUND OF THE INVENTION
There is an increasing demand in the cosmetics industry to develop products that may be applied topically to the skin that improve the condition and appearance of skin. Consumers are interested in mitigating or delaying the dermatological signs of chronologically or hormonally-aged or photo-aged skin, such as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis. During the aging process, the complexion of the skin, i.e., the color and appearance of the skin, deteriorates slowly from aging and/or exposure to sunlight. Numerous cosmetic and medical treatments have been developed in an attempt to treat aging or aged skin. Such cosmetics or treatments commonly contain organic acids as their active ingredients or components. These actives are frequently associated with consumer discomfort, such as burning, itching, and redness.
Therefore, there remains a general need in the cosmetics industry for products that retard or counter the aging effects on the skin, and more specifically for products that produce such effects without undesirable side effects. In particular, there remains a need for topically applied cosmetic compositions that have anti-aging and skin texture benefits using natural plant materials as active components.
Plumbago indica Linn. of the Plumbaginaceae family, also known as Red Plumbago and Jettamoonpleung have been employed for a variety of medicinal purposes. Scraped roots have been used in poultices where the poultices are applied to the head or area that is in pain or inflamed. The bark may also be used as an effective blistering plaster, and may be applied to the spine to treat certain fevers. Plumbago indica Linn. is also said to have anti-dyspeptic, anti-microbial, and anti-fertility activities. The Plumbago indica Linn. laurel is commonly found in tropical Asia and Thailand.
Cananga odorata Lamk. of the Annonaceae family, commonly known as Ylang-Ylang, Alanguilan, and Kradung-nga, and specifically its essential oils may be used in traditional medicine for relieving pain and for relaxation. Canango odorate Lamk. is distributed throughout the entire South Asian region, but principally as a cultivated plant. In particular, this tree is commonly found in all regions of Thailand.
Sapindus rarak of the Sapindaceae family commonly known as the Soap Nut tree and Ma Kam Dee Kao in Thai, may be used in traditional medicine. Decoction of Sapindus rarak fruits may be taken as an anti-pyretic and to relieve asthma. Hot water extracts of the fruits and flowers may also be used externally as an anti-fungal, anti-bacterial, anti-dandruff and against scalp diseases, and to reduce inflammation resulting from rashes. In addition to the aforementioned benefits, Sapindus rarak also has anti-parasitic and analgesic activities. Sapindus rarak commonly grows in a virgin forest up on a mountain mostly in the Northern part of Thailand.
Curcuma Xanthorrhiza Roxb. of the Zingiberaceae family, commonly known as Wild Turmeric and Wan Too Ra Hod in Thai, bears a striking similarity to Cape York Turmeric, only the flower bracts are a deeper pink/purple in color and have a dark stripe up the center of the leaf. However, Curcuma xanthorrhiza Roxb. is not the same as common turmeric or Khamin chan as it is known in Thai, although they bear a resemblance. Curcuma xanthorrhiza Roxb. has often been used medicinally as a drink by cooking dried slices of rhizome in water with palm sugar. Another herbal drink utilizes chopped up bulbs which are boiled in water and palm sugar. Shoots, flowers and rhizomes, as well as young parts of the plant, may be eaten raw or cooked. Asian cultures use this plant in mixtures for liver, gallstones, and indigestion complaints. Curcuma xanthorrhiza Roxb. has also been shown to aid in the reduction of cholesterol and cleansing the blood. The Curcuma plant family has been used for centuries in Thailand, Java, and Bali to beautify, perfume, tighten and deodorize the skin. Tumeric, or Curcuma xanthorrhiza Roxb, has been shown to have anti-bacterial, anti-fungal, and anti-viral properties. The key constituent of Curcuma xanthorrhiza Roxb., curcumin, reduces inflammation by up to about 50% and functions as an anti-oxidant.
Physalis minima Linn. of the Solanaceae family, also known as Sunberry, Gooseberry, and Tong Tang, is commonly used in traditional medicine. The fruit is considered to be a tonic, diuretic and purgative in the Punjab (India). The Mundas (a tribe) of Chhota-Nagpur mix the juice of the leaves with water and mustard oil and use it as a remedy against earaches. The Physalis minima plant has appetite stimulating, diuretic, laxative, and anti-inflammatory activities. The plant may also be used to treat enlargement of the spleen and various abdominal conditions. Physalis minima Linn. is commonly found in Thailand, particularly in the provinces of Chiang Mai, Angtong, Nakornsrithummarath, and Mahasarakham.
Stephania rotunda Lour. of the Menispermaceae family is a vined plant, and its common Thai name is Boorapet Pung Chang. Decoction of leaves and roots is taken as an appetite stimulant in traditional medicine. The flowers may be used externally for treating skin diseases such as ringworm and fungus infection. Stephania rotunda Lour. may also be used for ulcer treatment and is generally known to have anti-bacterial, anti-fungal, and anti-inflammatory activities. This plant commonly grows in Southern Thailand.
It would be desirable to have a topical composition comprising a natural plant material to improve the aesthetic appearance of skin. It would also be desirable to have an effective treatment for the dermatological signs of aging. Therefore, safe, effective and new compositions to treat, prevent, reduce, inhibit, and/or improve the dermatological signs of aging, would be advantageous for the formulation of treatments and products for the skin. As described herein, novel and beneficial methods and compositions, as well as their mode of action, for the treatment of wrinkles and the like, as well as for personal care products for the skin, are provided by the present invention.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide a topical cosmetic composition that delivers an effective level of an active component from a natural plant material.
It is another object of the present invention to provide a topical cosmetic composition having a natural plant material or blends of natural plant materials in a cosmetically, dermatologically, physiologically, or pharmaceutically acceptable vehicle.
It is a further object of the present invention to provide a topical composition having a natural plant material, or component therefrom, that results in increased expression of genes associated with dermatological signs of chronologically or hormonally-aged or photo-aged skin, such as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis, where the topical composition is a cosmetic product for the face and/or body.
It is yet another embodiment of the invention to provide a method of treating, preventing, and/or ameliorating the appearance of fine lines and/or wrinkles, comprising applying to skin a topical composition having a natural plant material in an amount effective to prevent, ameliorate and/or reduce dermatological signs of chronologically or hormonally-aged or photo-aged skin, such as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis.
It is still a further object of the present invention to provide a topical composition having one or more of the following natural plant materials: Plumbago indica, Cananga odorata, Sapindus rarak, Curcuma xanthorrhiza, Physalis minima, and Stephania rotunda, in an amount effective to treat, prevent, control, ameliorate, inhibit, and/or reduce dermatological signs of chronologically or hormonally-aged or photo-aged skin, such as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis, in order to improve the aesthetic appearance of skin.
Yet another object of the invention provides a topical cosmetic composition having a natural plant ingredient, which increases the expression levels of genes associated with the dermal-epidermal junction of skin, communication between the dermis and epidermis of skin, and mechanical properties of the skin, as well as, cell to cell cohesion in the epidermis of skin, anchoring of the cells of skin, communication between cells of skin, and tissue stability of skin, in order to improve the aesthetic appearance of skin.
These and other objects and advantages of the present invention, and equivalents thereof, are achieved by cosmetic compositions having a natural plant ingredient or blends thereof, and methods of use of such compositions for topical application in order to improve the aesthetic appearance of skin.


DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to topical compositions having an active component derived from a natural plant material. More particularly, one embodiment of the present invention relates to topical compositions having natural plant materials, including components or extracts derived therefrom, or blends of natural plant materials thereof, that improve the aesthetic appearance or condition of the skin and rejuvenate or enhance the skin by providing a variety of anti-aging and skin texture benefits. Improvements in the aesthetic appearance of the skin may be achieved by topical application of these cosmetic compositions to the skin on a daily basis.
One embodiment of the present invention relates to the novel use of natural plant materials, such as but not limited to, those from the Plumbaginaceae (plumbago), Annonaceae (Cananga), Sapindaceae (Sapindus); Zingiberaceae (Curcuma); Solanaceae (Physalis); and Menispermaceae (Stephania) families, or more specifically, the Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; and Stephania rotunda plants, respectively, in a topical cosmetic composition for application on the face and/or body in order to improve the condition and aesthetic appearance of skin.
In a further embodiment, the topical compositions having a natural plant material, or extract derived therefrom, may be used to treat, prevent, ameliorate, and/or reduce dermatological signs of chronologically aging, which represent the structural, functional, and metabolic changes in the skin that parallel the aging and degenerative changes in other body organs, or photo-aged skin, which is a separate process and largely involves damage to the collagen and elastin fibers in the skin, as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis, in order to improve the condition and aesthetic appearance of skin.
Symptoms of chronological aging include: dry and thin skin, fine wrinkles, abnormal blood vessels, age spots, and benign and malignant skin tumors. Young skin renews itself more frequently than older skin. The top layers thereby lose more moisture due to the aging process, and older skin has a dryer and more dehydrated appearance. Diminished production of collagen leads to fine wrinkles initially observed around the eyes (commonly known as “crow's feet”), forehead, and other sun-exposed areas. More pronounced effects include furrows at the site of facial expression lines and sagging folds over the eyelids, neck, jaw, and arms. Within the many small, delicate blood vessels supplying nutrients to the skin, abnormalities develop. This is particularly conspicuous over the nose and cheeks. Age spots are pigmentations that surfaced as a result of a deregulation of pigment cells in sun-exposed areas.
While genetics play a significant role, the number of wrinkles present is highly dependent on the amount of sun exposure. The lines in a “lived-in face,” especially for those who spend a considerable amount of time outdoors, is a consequence in part of oxidative damage due to overexposure to ultraviolet (UV) sunlight—both UVA (responsible for tanning, wrinkling, and melanoma) and UVB (responsible for sunburn and basal and squamous cell carcinoma). UV light may further damage skin by increasing the production of proteolytic enzymes that break down collagen, the connective tissue located beneath the dermis.
The skin is made up of multiple layers of cells that are constantly going through self shedding and regeneration once every 30 days in repeated cycles. The layers may be broadly divided into two sections—the top epidermis and the underlying dermis. Histological studies of the skin show that a wrinkle is formed following a series of major cellular changes. During the early phase of aging (from age 35-45), there is a gradual and progressive slowing of cellular turnover and regeneration. This results in the skin getting thinner. As a result, the normally undulating ridge-like dermal-epidermal junction (DEJ) becomes flatter. This flatness reduces the surface area of nutritional exchange between the underlying dermis on the bottom and the epidermis on top.
Reduced nutrition to the epidermis from aging is one factor that causes cellular exhaustion and weakness. Without proper nutrition to the epidermis, cellular metabolism of the epidermal cell is slowed. Furthermore, the transportation of certain unwanted byproducts of cellular metabolism such as free radicals are reduced. The accumulation of such free radicals within the cell may lead to undesirable mutational damages in the cell and ultimately cancer.
The adhesion in the DEJ is normally supplied by Collagen 4 (a multi-sheet structure or basal layer) and collagen 7 (anchored to the sheets structure). The progressive loss of nutrients to the DEJ slows the circulation of the messengers that serve to promote the neo-synthesis process of such collagen. Without an optimal amount of collagen, the skin sags even more, propagating the dearth of nutrients. Paradoxically, matured aging skin contains more elastin, which the body uses to fill in the empty space left by the deficiency of collagen. Such elastin, unfortunately, is fragmented, calcified, and contains excessive lipids. In addition to the loss of skin thickness due to the lack of collagen support, the aging or aged skin is more loose and lacks elasticity. These two properties are hallmarks of wrinkles. This process of aging and appearance of wrinkles is accelerated during the later phase of aging (age 45 and higher). By age 50, very few women can escape wrinkles.
Embodiments of the invention relate to the discovery that the natural plant materials identified in the present invention diminish skin lines and wrinkles, as well as relieves sagging, or other conditions due to a weakened skin matrix, in a topical cosmetic, composition, or formulation. The composition is preferably applied topically to the affected area of the skin once, twice, or more daily and allowed to remain on the affected area. The daily application may be left on the affected area of the skin for a period of at least one week, but may be for a period of up to two, four, eight, twelve weeks or more to improve the condition and aesthetic appearance of skin.
In particular, the natural plant material, such as but not limited to, Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; and Stephania rotunda, including components or extracts derived therefrom, increase expression of one or more of the following genes: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase, such that when the composition having the natural plant material is administered topically, improves the condition and aesthetic appearance of skin due to dermatological signs of aging by lessening facial lines, wrinkles, and sagging skin. The topical composition is preferably applied and left on the affected area in a daily manner.
Topical Composition
For purposes of the invention, the natural plant material may be in any form including, but not limited to, the whole plant, a dried plant, a ground plant, or parts thereof, including but not limited to, seeds, needles, leaves, roots, bark, cones, stems, rhizomes, callus cells, protoplasts, organs and organ systems, and meristems, or components and/or constituents found in, or isolated from, the natural plant material, and/or portions of the plant, or extracts derived either directly or synthetically from the plant, or any combinations thereof. A preferred natural plant material is in the form of an extract derived from the natural plant material.
It is to be understood that “natural plant material” also includes an ingredient, component, constituent, or extract derived from the natural plant material.
In one embodiment of the invention, the topical compositions having a natural plant material selected from one or more of the plants: Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; and Stephania rotunda are useful for improving the condition and aesthetic appearance of skin, particularly matured or maturing skin, by any one of the following methods: reducing dermatological signs of chronological aging, photo-aging, hormonal aging, and/or actinic aging; preventing and/or reducing the appearance of lines and/or wrinkles; reducing the noticeability of facial lines and wrinkles, facial wrinkles on the cheeks, forehead, perpendicular wrinkles between the eyes, horizontal wrinkles above the eyes, and around the mouth, marionette lines, and particularly deep wrinkles or creases; preventing, reducing, and/or diminishing the appearance and/or depth of lines and/or wrinkles; improving the appearance of suborbital lines and/or periorbital lines; reducing the appearance of crow's feet; rejuvenating and/or revitalizing skin, particularly aging skin; reducing skin fragility; preventing and/or reversing of loss of glycosaminoglycans and/or collagen; ameliorating the effects of estrogen imbalance; preventing skin atrophy; preventing, reducing, and/or treating hyperpigmentation; minimizing skin discoloration; improving skin tone, radiance, clarity and/or tautness; preventing, reducing, and/or ameliorating skin sagging; improving skin firmness, plumpness, suppleness and/or softness; improving procollagen and/or collagen production; improving skin texture and/or promoting retexturization; improving skin barrier repair and/or function; improving the appearance of skin contours; restoring skin luster and/or brightness; minimizing dermatological signs of fatigue and/or stress; resisting environmental stress; replenishing ingredients in the skin decreased by aging and/or menopause; improving communication among skin cells; increasing cell proliferation and/or multiplication; increasing skin cell metabolism decreased by aging and/or menopause; retarding cellular aging; improving skin moisturization; enhancing skin thickness; increasing skin elasticity and/or resiliency; enhancing exfoliation; improving microcirculation; decreasing and/or preventing cellulite formation; and any combinations thereof.
As stated above, the preferred natural plant materials for use in the present invention are selected from one or more of the following plants: Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; and Stephania rotunda. However, it is also contemplated that other natural plant materials may work equally as well if they increase expression levels of genes associated with dermatological signs of aging. These genes may include, but are not limited to, Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase.
In another embodiment, the composition may have an extract derived from a natural plant material in an amount from about 0.0001% to about 50%, preferably from about 0.001% to about 20%, more preferably from about 0.01% to about 5%, and most preferably from about 0.05% to about 1%, based on the total weight of the composition, where the composition is useful in improving the condition and aesthetic appearance of skin.
The natural plant material is derived from any one, or combinations thereof, of plants: Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; and Stephania rotunda, or any natural plant material that increases expression levels of any one, or combinations thereof, of genes: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase. The natural plant materials which increase expression levels of genes associated with dermatological signs of chronologically or hormonally-aged or photo-aged skin, such as fine lines, wrinkles, and sagging skin, and other conditions due to a progressive degradation of the dermal-epidermal junction (DEJ) and of the cell-cell cohesion in the epidermis, in order to improve the condition and aesthetic appearance of skin, and the composition comprising one or more of these plants are effective when topically applied, preferably in a daily manner. Without wishing to be bound by theory, the natural plant materials, which increase gene expression of the aforementioned genes, exert their effects through a mechanism of action involving the dermal-epidermal junction of skin, communication between the dermis and epidermis of skin, and mechanical properties of the skin, as well as, cell to cell cohesion in the epidermis of skin, anchoring of the cells of skin, communication between cells of skin, and tissue stability of skin. Topical application of the plant also facilitates the targeted delivery of the active components without the requirement of an injection or the expertise of a health practitioner.
The active components of the present invention are further useful in treating, which includes preventing, arresting, ameliorating, reducing or diminishing, medical and/or cosmetic conditions of the skin. Such conditions, as used herein, commonly include, but are not limited to, dermatological aging (chronological aging, hormonal aging and/or actinic aging), dermatitis, skin and hair fragility, hirsutism, rosacea, skin blemishes, sensitive skin, hyperpigmentation or hypopigmentation, thinning skin, roughness, keratosis, skin atrophy, wrinkles, lines, hyperplasia, fibrosis, and any combinations thereof. The active components of the present invention may also be useful in enhancing the general health, vitality, condition, and appearance of the skin.
Topical compositions having the aforementioned active components of a natural plant material, where the active components increase expression levels of genes, including, but not limited to: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase, improve the condition, cosmetic, and/or aesthetic appearance of skin.
Wnt proteins form a family of highly conserved secreted signaling molecules that regulate cell-to-cell interactions during embryogenesis. Wnt genes and Wnt signaling are also implicated in cancer. Insights into the mechanisms of Wnt action have emerged from several systems, including, genetics in Drosophila and Caenorhabditis elegans; as well as, biochemistry in cell culture and ectopic gene expression in Xenopus embryos. Many Wnt genes in the mouse have been mutated, leading to very specific developmental defects. Wnt proteins bind to receptors of the Frizzled family (FZ) on the cell surface. Through several cytoplasmic relay components, the signal is transduced to beta-catenin, which then enters the nucleus and forms a complex with transcription factor (TCF) to activate transcription of Wnt target genes.
Frizzled (Fz) genes encode a family of Wnt ligand receptors, which have a conserved cysteine-rich Wnt binding domain and include both transmembrane and secreted forms. Disturbing Wnt signaling results in aberrant hair formation, hair growth, and hair structure. Elevated expression of Fz10 was confined to developing hair follicles (Reddy, et al. “Expression of Frizzled Genes in Developing and Postnatal Hair Follicles.” Journal of Investigative Dermatology 123 (2), 275-282, 2004; incorporated by reference).
Beta-catenin is a cytoskeletal component that enters the nucleus to act as a transcriptional cofactor. Phosphorylation of Beta-catenin induces the ubiquitination and proteolytic degradation of beta-catenin by the proteasome. Non-phosphorylated beta-catenin is stable and enters the nucleus to regulate transcription with TCF. The beta-catenin/TCF complex thereby activates genes that promote cellular survival, proliferation and differentiation during development.
Collagen, which has several forms, is a major body protein and accounts for almost 20% of the total protein in the human body. In addition to its critical role in forming support structure of the skin, it is also the main constituent of the extra-cellular matrix. Mutations in Collagen 7 are known to be involved in many types of epidermolysis bullosa, a human skin disease characterized by separation of dermal and epidermal layers (Pulkkinen L, et al. “Mutation analysis and molecular genetics of epidermolysis bullosa.” Matrix Biol 18:29-42, 1999; incorporated by reference). Collagen 4 is associated with extracellular matrix and basement membrane zone formation, which is responsible for epidermal adhesion to the dermis in human skin.
Activation of ERα is responsible for many biological processes, including cell growth and differentiation, morphogenesis and programmed cell death (Katzenellenbogen, B. S. “Estrogen receptors: bioactivities and interactions with cell signaling pathways” Biol. Reprod., 54, 287-293, 1996; Katzenellenbogen, B. S., et al. “Antiestrogens: mechanisms of action and resistance in breast cancer” Breast Cancer Res. Treat., 44, 23-38, 1997; both incorporated by reference). In addition, ERa plays an important role in the development and progression of breast cancer by regulating genes and signaling pathways involved in cellular proliferation.
Hyaluronic acid (HA) is a polysaccharide found in the extracellular mactrix of vertebrate tissues and in the surface coating of certain Streptococcus and Pasteurella bacterial pathogens. HA synthases (HASs) are the enzymes that polymerize HA. HA is known to be secreted out of the cell; and accordingly, HASs are normally found in the outer membranes of the organisms.
Increases in expression levels of specific genes associated with the progressive degradation of the dermal-epidermal junction (DEJ), including poor communication between the dermis and epidermis, poor mechanical properties of the skin; and of the cell-cell cohesion, including poor anchoring of the cells, poor cell-cell communication and poor tissue stability, in the epidermis are particularly useful in the topical compositions of the present invention. In one embodiment, the useful genes include, but are not limited to, Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic Acid Synthase. Any natural plant material, including an extract derived therefrom, that increases expression of any one of these genes, is found useful in the topical compositions of the present invention.
Gene expression may be measured by the determination of RNA levels in cultured cells, for example, using techniques such as Northern blot technology and the polymerase chain reaction (PCR), e.g., “real time” PCR and reverse transcription PCR (RT PCR) as practiced in the art. (see, e.g., J. Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.; R. Higuchi et al., 1992, Biotechnology, 10:413-417; R. Higuchi et al., 1993, Biotechnology, 11: 1026-1030; E. S. Kawasaki, 1990, “Amplification of RNA”, In: RNA Protocols: A Guide to Methods & Applications, M. A. Innis et al., Academic Press, San Diego, Calif., pp. 21-27; all of which are herein incorporated by reference). In addition, gene expression in skin, skin substitute, or cultured cells may be evaluated using gene (cDNA) arrays (microarrays or nucleic acid genechip test arrays comprising membrane, glass, or plastic support materials), serial analysis of gene expression (SAGE), (e.g., as described by V. E. Velculescu et al., Science, 270(5235):484-487, 1995; A. Lal et al., Cancer Res., 59(21):5403-5407, 1999; both of which are herein incorporated by reference), or differential display techniques. Example 3 shows the results of gene expression of Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic Acid Synthase upon treatment with natural plant materials.
The invention encompasses using genes associated with dermatological signs of aging, such as fine lines, wrinkles, and sagging skin as a biomarker for compounds which may improve the condition and appearance of skin. If expression levels of such nucleic acid biomarkers are elevated in the presence of a natural plant material, the natural plant material may be used in a topical composition of the invention for improving the condition and appearance of skin. Such assays embrace a variety of methods for measuring nucleic acid levels in cells that have been exposed to one or more test substances. Suitable methods include detection and evaluation of gene activation or expression of, for example, DNA, RNA, or mRNA. As non-limiting examples, polymerase chain reaction (PCR) assays (e.g., RT-PCR), Northern blotting, in situ hybridization, and other assays as known and practiced in the art may be employed to quantify RNA in cells being assayed for tolerance to a particular treatment (see, e.g., J. O'Connell, 2002, RT-PCR Protocols, Humana Press, Totowa, N.J.; R. Rapley and D. L. Manning, 1998, RNA Isolation and Characterization Protocols, Humana Press; R. Rapley, 2000, Nucleic Acid Protocols Handbook, Humana Press; all of which are herein incorporated by reference). In accordance with such assays, if levels of at least one nucleic acid biomarker are elevated in the presence of one or more test substances, this may predict that the substance(s) will improve the dermatological signs of aging. These substances, or natural plant materials, may then be used in a topical composition, preferably applied daily to the skin, in order to treat, prevent, ameliorate, and/or reduce, signs of dermatological aging, especially fine lines, wrinkles, and sagging skin, thereby improving the condition and aesthetic appearance of skin.
In a further embodiment, the natural plant material as used herein, also includes “synthetic” extracts, i.e. various combinations of known the natural plant material components and/or constituents that are combined to substantially mimic the composition and/or activity of the natural plant material. Such synthetic extracts are included in the term “natural plant material extract.” Most preferably, the synthetic extracts have substantially the same number of active components as a natural plant material. The correspondence of the numerical incidence of actives between the synthetic extracts and the natural plant material may also be described in terms of “percent commonality.”
Another embodiment relates to a synthetic extract having about 50% or more commonality to the chemical composition of a natural plant material. For example, the synthetic extract has about 50% or more of the active components found in the natural plant material. The chemical composition of the synthetic extract may have about 70% or more commonality to the chemical composition of the natural plant material. A synthetic extract may have about 90% or more commonality to the chemical composition of the natural plant material.
For use in the compositions of the invention, the natural plant material and/or active components are derived directly from the natural plant material, including the entire plant, its seeds, needles, leaves, roots, bark, cones, stems, rhizomes, callus cells, protoplasts, organs and organ systems, and meristems. The components may be in a pure form, a semi-pure form, or unpurified form. The natural plant material in the form of an extract may be obtained by extracting either the entire plant, or various parts thereof, with 80% ethanol.
Briefly, the organic solvent extraction method involves washing and extracting the plant material using an organic solvent. Non-limiting examples of organic solvents include methanol, ethanol, isopropanol, dichloromethane, chloroform, hexane, xylene, and petroleum ether. An extracting machine may be used for organic solvent extraction as is well known in the field.
Organic solvent extraction involves collecting the raw materials from the plant that contain the desired constituent(s), such as seeds, needles, leaves, roots, bark, cones, stems, rhizomes, callus cells, protoplasts, organs and organ systems, and meristems. These plant materials are ground to small particle sizes, and then put into an extracting machine through an inlet for the raw materials by a measurable charging machine. The plant raw material is pushed in the extracting machine by a thruster, and slowly moves the plant raw material forward. Organic solvent (e.g., ethanol) may be added into the machine through a solvent inlet at the top of a waste discharge outlet. Due to the difference in gravity and equilibrium, the solvent flows toward the raw material inlet, soaks the materials and flows out from the opposite side of the solvent inlet. Since the plant materials and the solvent move in opposite directions against each other, the plant materials are constantly immersed in a solution that contains a low-concentration of extract. As a result of equilibrium, high yield of plant constituent(s) may be achieved by continuously extracting the plant material against the low-concentration solution.
An extraction time between about 1-8 hours is suitable, and more preferably is between about 2-6 hours, and most preferably is between about 3-5 hours. The temperature of extraction is between about 30° C.-90° C., preferably between about 40° C.-70° C., and more preferably between about 50° C.-60° C. The collected extract is then fine-filtered to remove debris, and may be used directly, or is concentrated, for example, by distilling the solvent or by other conventional processing, where the extract may also be provided as a dry powder.
Similarly, aqueous-organic solvent extraction involves initially collecting raw materials from a plant containing the desired alkaloid(s), such as seeds, needles, leaves, roots, bark, cones, stems, rhizomes, callus cells, protoplasts, organs and organ systems, and meristems of the plant, which are ground into small particle sizes. The ground plant material is soaked in aqueous solution that is acidic or alkaline, depending on the solubility and stability of the desired extract under acidic or alkaline (basic) conditions. For extraction under acidic conditions, an acid such as hydrochloric acid or sulfuric acid is added to water, e.g., at a concentration of about 3% (w/v). For extraction under alkaline conditions, an alkali such as sodium hydroxide or sodium carbonate is added to water. The extraction time and temperature of extraction are typically similar to that used in the organic solvent extraction method described above.
The extract is then collected and fine-filtered to remove debris. Alkaline agents (e.g., ammonia) or acidifying agents (e.g., sulfuric acid) may be added to the extract to neutralize the solution by adjusting the pH, depending on the acidity or alkalinity of the collected extract. The aqueous extract may be used directly, concentrated or dried. Alternatively, organic solvent may then be added to the neutralized solution to transfer the extract actives from an aqueous phase to an organic phase. Non-limiting examples of such organic solvents include, but are not limited to, ethanol, isopropanol, butanol, pentanol, hexanol and xylene. The extract comprising the transferred extract actives dissolved in organic solvent may be used directly, used as a concentrate, or dried.
Different plants containing different constituents may be mixed and extracted together. This process of mixed extraction may preferably be used for extracting those plants containing constituents having similar solubility in the solvent used for extraction, such as ethanol. The mixture of extracts may be concentrated and stored in an appropriate solvent.
Formulations
In accordance with the invention, compositions comprising components from the natural plant material include, but are not limited to, topically applied formulations, anti-oxidants, anti-inflammatories, sunscreens, cosmetics, including makeup, formulations for reducing dermatological signs of aging, including wrinkles, fine lines, and sagging skin, topicals, skin penetration enhancers, and the like. Also in accordance with this invention, the natural plant material, components, and additional constituents comprising such compositions may be formulated in a variety of product forms. The compositions may be prepared in targeted delivery systems, e.g. creams, lotions, masks, gels, toners, serums, transdermal patches, and the like, particularly for topical administration. Targeted delivery and/or penetration enhancement may also be achieved by iontophoresis.
The present invention further provides the compositions comprising the natural plant materials preferably for topical administration or for targeted delivery without inducing significant irritation. The inventive compositions are suitable for all skin types, such as sensitive, normal, dry, or oily, preferably sensitive to dry skin. In particular embodiments, the compositions may be suitable for dry skin. The compositions are applied to the skin for a period of time sufficient to improve the condition or aesthetic appearance of skin. The topical compositions may be applied topically once, twice, or more daily. The daily application may be applied for a period of one week, two weeks, four weeks, or more. The preferred topical composition or formulation may be applied and left on the affected area once daily.
The topical compositions may be formulated into liposomes which may comprise other additives or substances, and/or which may be modified to more specifically reach or remain at a site following administration. The compositions of embodiments of the present invention yield improvements to the conditions and aesthetic appearance of skin by treating at least one of the previously described conditions, or combinations thereof.
Another embodiment of the invention encompasses compositions comprising a cosmetically or dermatologically acceptable formulation which is suitable for contact with living animal tissue, including human tissue, with virtually no adverse physiological effect to the user. Compositions embraced by this invention can be provided in any cosmetically and/or dermatologically suitable form, preferably as a lotion or cream, but also in an anhydrous or aqueous base, as well as in a sprayable liquid form. Other suitable cosmetic product forms for the compositions of this invention include, for example, an emulsion, a cream, a balm, a gloss, a lotion, a mask, a serum, a toner, an ointment, a mousse, a patch, a pomade, a solution, a spray, a wax-based stick, or a towelette. In addition, the compositions contemplated by this invention can include one or more compatible cosmetically acceptable adjuvants commonly used and known by the skilled practitioner, such as colorants, fragrances, emollients, humectants, preservatives, vitamins, chelators, thickeners, perilla oil or perilla seed oil (WO 01/66067 to a “Method of Treating a Skin Condition,” incorporated herewith) and the like, as well as other botanicals such as aloe, chamomile, and the like, and as further described below.
Also, embraced by the invention are transdermal modes of delivery, such as patches and the like, with or without a suitable penetration enhancers. The methods and compositions embodied by the invention provide a means by which the natural plant material components may be effectively administered in a transdermal system. Accordingly, a transdermal means of delivering a composition or formulation (often with a penetration enhancing composition) to the skin is that of the transdermal patch or a similar device as known and described in the art. Examples of such devices are disclosed in U.S. Pat. Nos. 5,146,846; 5,223,262; 4,820,724; 4,379,454; and 4,956,171, all of which are incorporated herein by reference and such descriptions are not meant to be limiting. The transdermal mode of storing and delivering the compositions onto the skin, including hair, and forming the active composition is convenient and well-suited for the purposes of an embodiment of the present invention. In a preferred method, the application is through a sustained release vehicle, e.g., a topically applied sustained released patch. Preferably, when a topical patch is used, the patch is applied to the desired area for extended period of time. Preferably, the extended period of time is greater than one hour, most preferably the extended period of time is overnight, i.e., when the user is sleeping.
The natural plant material component(s) of the present invention are preferably contained in a cosmetically, dematologically, physiologically, and pharmaceutically acceptable vehicle, medium, diluent or carrier, for use in treating, reducing, ameliorating, or preventing conditions associated with excess accumulation, production, or excretion of sebum and excess accumulation or production of subcutaneous fat.
In an embodiment embracing topical application, the compositions of this invention comprise a medium (vehicle, diluent or carrier) that is compatible with human skin, including hair. The compositions can be formulated as an aqueous phase, an oil phase, alcohol, or aqueous/alcohol-based solutions, ointments, lotions, gels, a wax-in-water emulsion, or water-in-oil, oil-in-water, of water-oil-water triple emulsions having the appearance of a cream or gel, microemulsions, or aerosols.
The aqueous phase is a mixture of one or more water soluble or water dispersible ingredient, which can be liquid, semi-solid or solid at room temperature (25° C.). The vehicle comprises or can be in the form of a suspension, dispersion or solution in water or an aqueous-alcoholic vehicle, which may contain a thickener or gellant. A person skilled in the art can select the appropriate cosmetic form, the ingredients contained therein, as well as the method for preparing it, on the basis of the knowledge that the skilled artisan possesses.
In one embodiment, the composition may include an aqueous phase which may contain water or a mixture of water and at least one hydrophilic organic solvent in particular an alcohol, especially a linear or branched lower monoalcohol containing from 2 to 5 carbon atoms, e.g., ethanol or propanol; a polyol, e.g., propylene glycol, sorbitol, glycerol, diglycerol, panthenol, or polyethylene glycol, and mixtures thereof. This aqueous phase may represent from about 0.5 weight % to about 99.99 weight %, based upon the total weight of the composition.
Yet another embodiment when the composition of the invention is in the form of an emulsion, the composition may also optionally comprise a surfactant, preferably in an amount of from about 0.1 weight % to about 30 weight %, and in particular, from about 1 weight % to about 20 weight %, based upon the total weight of the composition.
In a further embodiment of the invention, the composition may also comprise a thickening polymer such as an amphiphilic polyurethane, a polyacrylic homopolymer or copolymer, a polyester, or a hydrocarbon-based resin.
One embodiment of the invention further relates to a composition of the invention which may also comprise an oil phase containing oil soluble or oil dispersible ingredients that are liquid at room temperature (25° C.) and/or oily or waxy substances that are solid at room temperature, such as waxes, semi-solids, gums, and mixtures thereof. This oily phase may also contain organic solvents.
Suitable oily materials that are liquid at room temperature, often referred to as oils, include hydrocarbon-based oils of animal origin such as perhydrosqualene; hydrocarbon-based plant oils such as liquid triglycerides of fatty acids of 4 to 10 carbon atoms, for instance, heptanoic or octanoic acid triglycerides, or oils such as sunflower oil, corn oil, soybean oil, grapeseed oil, castor oil, avocado oil, caprylic/capric acid triglycerides, jojoba oil; linear or branched hydrocarbons of mineral or synthetic origin such as liquid paraffins and derivatives thereof, petroleum jelly; synthetic esters and ethers, in particular esters of fatty alcohols, namely; for example, isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, isostearyl isostearate; hydroxylated esters such as isostearyl lactate, octyl hydroxystearate, octyldodecyl hydroxystearate, heptanoates, octanoates and decanoates of fatty alcohols; polyol esters such as propylene glycol dioctanoate, neopentyl glycol diheptanoate, diethylene glycol diisononanoate, and pentaerythritol esters; fatty alcohols containing from 12 to 26 carbon atoms such as octyldodecanol, 2-butyloctanol, 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol; partially hydrocarbon-based fluoro oils and/or fluorosilicone oils; silicone oils such as volatile or non-volatile, linear or cyclic polydimethylsiloxanes (PDMS) that are liquid or semisolid at room temperature such as cyclomethicones and dimethicones, optionally comprising a phenyl group, for instance phenyl trimethicones, siloxanes, and mixtures thereof. These oils are usually present in an amount of about 0 weight % to about 90 weight %, preferably from about 1 weight % to about 80 weight % by weight of the oil phase.
The oil phase of the composition of the invention may also comprise one or more cosmetically acceptable organic solvents. These solvents are present in an amount of about 0 weight % to about 60 weight %, preferably about 1 weight % to about 30 weight %, based on the total weight of the composition, and may be selected from the group consisting of lipophilic organic solvents, amphiphilic organic solvents and mixtures thereof. Suitable solvents which may be used in the composition of the invention include acetic acid esters such as methyl, ethyl, butyl, amyl or 2-methoxyethyl acetate; isopropyl acetate; hydrocarbons such as toluene, xylene, p-xylene, hexane or heptane; ethers containing at least 3 carbon atoms, and mixtures thereof.
The composition of the invention may further comprise any ingredient conventionally used in the cosmetics field. These ingredients include preserving agents, aqueous phase thickeners (polysaccharide biopolymers, synthetic polymers) and fatty-phase thickeners, fragrances, hydrophilic and lipophilic active agents, and mixtures thereof. The amounts of these various ingredients are those conventionally used in the cosmetics field to achieve their intended purpose, and range typically from about 0.01 weight % to about 20 weight %, based upon the total weight of the composition. The nature of these ingredients and their amounts must be compatible with the production of the compositions of the invention.
The composition of the invention may also comprise an additional particulate phase, typically present in an amount of about 0 weight % to about 30 weight %, based upon the total weight of the composition, preferably from about 0.05 weight % to about 20 weight %, and which can comprise pigments and/or pearlescent agents and/or fillers used in cosmetic compositions. Suitable inorganic pigments include, but are not limited to, titanium oxide, zirconium oxide and cerium oxide, as well as zinc oxide, iron oxide, chromium oxide and ferric blue. Suitable organic pigments include, but barium, strontium, calcium, and aluminium lakes and carbon black. Suitable pearlescent agents include mica coated with titanium oxide, with iron oxide, or with natural pigment.
Fillers are normally present in an amount of about 0 weight % to about 30 weight %, based on the total weight of the composition, preferably about 0.5 weight % to about 15 weight %. Suitable fillers include talc, silica, zinc stearate, mica, kaolin, nylon (in particular orgasol) powder, polyethylene powder, Teflon®, starch, boron nitride, copolymer microspheres such as Expancel® (Nobel Industrie; Sweden), Polytrap® (Dow Coming, Inc.; Midland, Mich.), and silicone resin microbeads (Tospearl®; GE Toshiba Silicones; Japan).
The oil phase of the compositions of the invention may comprise one or more waxes, gums, or mixtures thereof. The waxes include hydrocarbon-based waxes, fluoro waxes and/or silicone waxes and can be of plant, mineral, animal and/or synthetic origin. In particular, the waxes have a melting point of greater than 25° C., preferably greater than 45° C. The compositions of the present invention may contain from about 0 weight % to about 20 weight % waxes, based upon the total weight of the composition.
The gums are generally high molecular weight polydimethylsiloxanes (PDMSs), cellulose gums or polysaccharides, and the semi-solid materials are generally hydrocarbon-based compounds, such as, but not limited to, lanolins and derivatives thereof, or alternatively PDMSs. The compositions of the present invention may contain from about 0 weight % to about 20 weight % gums, based upon the total weight of the composition, typically from about 0.1 weight % to about 10 weight %.
More particularly, the compositions for topical application can be in the form of a protective care composition for the skin, preferably for the face, the neck, the hands, the feet, or other areas of the body. Non-limiting examples include day creams or lotions, night creams or lotions, salves, sunscreen creams, fluid lotions, oils, ointments, gels, masks, body milks, makeup (a foundation), artificial tanning compositions, depilatories, emulsifiers, patches, or a solid which is poured or cast as a stick or a dish, for example.
Another particular embodiment of the present invention is directed to the delivery of the described compositions comprising a natural plant material by targeted delivery systems, for example, liposomes, microspheres (see, e.g., U.S. Pat. No. 5,770,222 to Unger et al., incorporated herein by reference), and the like, so that the natural plant material compositions and/or active constituents may more readily reach and affect the dermal-epidermal junction layer of the area where the composition is topically applied, e.g., face or neck, or other affected areas of the skin.
In another embodiment, the topical compositions of the present invention may also include one or more of the following: a skin penetration enhancer, an emollient, a skin plumper, an optical diffuser, a sunscreen, an exfoliation promoter, and an antioxidant. Details with respect to these and other suitable cosmetic ingredients can be found in the International Cosmetic Ingredient Dictionary (ICID) and Handbook, 10th Edition (2004), published by the Cosmetic, Toiletry, and Fragrance Association (CTFA), at pp. 2177-2299, which is herein incorporated by reference in its entirety.
An emollient provides the functional benefits of enhancing skin smoothness, reducing the appearance of fine lines and coarse wrinkles, and moisturizing. Non-limiting examples include isopropyl myristate, petrolatum, isopropyl lanolate, silicones (e.g., methicone, dimethicone), oils, mineral oils, fatty acid esters, or any mixtures thereof. The emollient is preferably present from about 0.1 weight % to about 50 weight % of the total weight of the composition.
A skin plumper serves as a collagen enhancer to the skin. An example of a suitable, and preferred, skin plumper is palmitoyl oligopeptide. Other skin plumpers are collagen and/or glycosaminoglycan (GAG) enhancing agents. The skin plumper is preferably present from about 0.1 weight % to about 20 weight % of the total weight of the composition.
An optical diffuser is a particle that changes the surface optometrics of skin, resulting in a visual blurring and softening of, for example, lines and wrinkles. Examples of optical diffusers that can be used in the present invention include, but are not limited to, boron nitride, mica, nylon, polymethylmethacrylate (PMMA), polyurethane powder, sericite, silica, silicone powder, talc, Teflon, titanium dioxide, zinc oxide, or any mixtures thereof. The optical diffuser is preferably present from about 0.01 weight % to about 20 weight % of the total weight of the composition.
A sunscreen protects the skin from damaging ultraviolet rays. In an illustrative embodiment of the invention, the sunscreen would provide both UVA and UVB protection, by using either a single sunscreen or a combination of sunscreens. Among the sunscreens that can be employed in the present compositions are avobenzone, cinnamic acid derivatives (such as octylmethoxy cinnamate), octyl salicylate, oxybenzone, titanium dioxide, zinc oxide, or any mixtures thereof. The sunscreen may be present from about 1 weight % to about 30 weight % of the total weight of the composition. The addition of a sunscreen may protect the skin from ultraviolet radiation.
The compositions of the invention having sunscreen bring about additional improvements to the aesthetic appearance of skin, including at least one of the following: minimizes sunburning, minimizes tanning, and reduces redness.
In an embodiment of the invention, compositions may also have one or more exfoliation promoters. Suitable examples of an exfoliation promoter that can be used in the present compositions include alpha hydroxy acids (AHA); benzoyl peroxide; beta hydroxy acids; keto acids, such as pyruvic acid, 2-oxopropanoic acid, 2-oxobutanoic acid, and 2-oxopentanoic acid; oxa acids as disclosed in U.S. Pat. Nos. 5,847,003 and 5,834,513 (the disclosures of which are incorporated herein by reference); salicylic acid; urea; or any mixtures thereof. The preferred exfoliation promoters are 3,6,9-trioxaundecanedioic acid, glycolic acid, lactic acid, or any mixtures thereof. (See also, ICID at p. 2205).
When an embodiment of the invention includes an exfoliation promoter, the composition has about 0.1 weight % to 30 weight %, preferably about 1 weight % to about 15 weight % and more preferably about 1 wt % to about 10 wt %, of the exfoliation promoter based on the total weight of the composition.
An antioxidant functions, among other things, to scavenge free radicals from skin to protect the skin from environmental aggressors. Examples of antioxidants that may be used in the present compositions include compounds having phenolic hydroxy functions, such as ascorbic acid and its derivatives/esters; beta-carotene; catechins; curcumin; ferulic acid derivatives (e.g. ethyl ferulate, sodium ferulate); gallic acid derivatives (e.g. propyl gallate); lycopene; reductic acid; rosmarinic acid; tannic acid; tetrahydrocurcumin; tocopherol and its derivatives; uric acid; or any mixtures thereof. Other suitable antioxidants are those that have one or more thiol functions (—SH), in either reduced or non-reduced form, such as glutathione, lipoic acid, thioglycolic acid, and other sulfhydryl compounds. The antioxidant may be inorganic, such as bisulfites, metabisulfites, sulfites, or other inorganic salts and acids containing sulfur. Compositions of the present invention may have an antioxidant preferably from about 0.001 wt % to about 10 wt %, and more preferably from about 0.01 wt % to about 5 wt %, of the total weight of the composition. (See also, ICID at p. 2184).
In an embodiment of the invention, the composition may also have one or more of the following cosmetic and pharmaceutical active agents, excipients, ingredients, or adjuvants: anesthetics, antibiotics, e.g., erythromycins and tetracyclines, salicylic acids, anti-allergenics, antifungals, antiseptics, anti-irritants, anti-inflammatory agents, antimicrobials, analgesics, nitric oxide synthase inhibitors, insect repellents, self-tanning agents, skin penetration enhancers, skin cooling agents, chelating agents, colorants including dyes, lakes and pigments that may be untreated or chemically surface treated to improve wetability or some other property, demulcents, emollients, emulsifiers, fragrances, humectants, lubricants, skin protectants, moisturizers, pH adjusters, preservatives, stabilizers, surfactants, thickeners, film formers, plasticizers, viscosity modifiers, vitamins, or any mixtures thereof. The amounts of these various substances are those that are conventionally used in the cosmetic or pharmaceutical fields to achieve their intended purposes, for example, they may constitute from about 0.01% to 20% of the total weight of the composition.
Non-limiting examples of active agents for formulating into the compositions of the invention include those reagents having an effect on the treatment of wrinkles and/or fine lines, in addition to the actives as described, such as keratolytic agents, i.e., an active agent having desquamating, exfoliant, or scrubbing properties, or an active agent which can soften the horny layer of the skin. Other examples of anti-wrinkle or anti-fine line active agents include hydroxy acids and retinoids. These agents can be formulated, for example, in amounts of from about 0.0001% to 5% by weight relative to the total weight of the composition.
Suitable hydroxy acids include, for example, glycolic acid, lactic acid, malic acid, tartaric acid, citric acid, 2-hydroxyalkanoic acid, mandelic acid, salicylic acid and alkyl derivatives thereof, including 5-n-octanoylsalicylic acid, 5-n-dodecanoylsalicylic acid, 5-n-decanoylsalicylic acid, 5-n-octylsalicylic acid, 5-n-heptyloxysalicylic acid, 4-n-heptyloxysalicylic acid and 2-hydroxy-3-methylbenzoic acid or alkoxy derivatives thereof, such as 2-hydroxy-3-methyoxybenzoic acid.
Emulsifiers are typically present in the compositions of the invention in an amount of about 0.01 weight % to 30 weight %, by weight and preferably from about 0.1 weight % to 30 weight % by weight relative to the total weight of the composition. However, not all compositions will necessarily include emulsifiers. (See e.g., ICID at p. 2276-2285).
Non-limiting examples of suitable thickening agents include xanthan gum, hydroxypropyl cellulose, hydroxyethyl cellulose, carbomer, gum acacia, Sepigel 305 (available from Seppic Co., France), and clays such as magnesium aluminum silicate. (See, e.g., ICID at p. 2293-2299).
The topical compositions of the present invention may include, and their utility can be enhanced by one or more humectants, such as ureas, pyrrolidone carboxylic acids, amino acids, sodium hyaluronates, certain polyols and other compounds with hygroscopic properties. (See ICID at p. 2244).
The general activity and mildness to skin of the present topical compositions can also be enhanced by neutralization to pH about 3.5 to about 7.0, most preferably from pH about 3.7 to about 5.6. This neutralization is preferably accomplished with one or more of ammonium hydroxide, potassium hydroxide, sodium hydroxide, arginine or other amino acids, and/or triethanolamine.
Exemplary retinoids include, without limitation, retinoic acid (e.g., all-trans or 13-cis) and derivatives thereof, retinol (Vitamin A) and esters thereof, such as retinol palmitate, retinol acetate and retinol propionate, and salts thereof.
The natural plant materials, or extracts therefrom, of the present invention are preferably contained in a cosmetically or dematologically acceptable vehicle, medium, diluent or carrier. The topical composition comprising the natural plant materials may be further formulated according to procedures known in the art to provide cosmetic compositions such as emulsions, gels, creams, lotions, masks, toners, serums, oils, water-in-oil, oil-in-water, water-oil-water triple emulsions having the appearance of a cream or gel, microemulsions, ointments, pastes, sticks, cakes, pencils, aerosol, and essences, as well as other topical cosmetic vehicles. It is also contemplated that topical compositions of the present invention can be incorporated into delivery systems such as liposomes and topical patches, tapes, and sprays.
In addition, the compositions may be in the form of vesicular dispersions containing ionic and/or nonionic lipids, as described above. Dosage units suitable for such compositions are formulated according to the conventional knowledge and techniques used in the art.
In a further embodiment of the invention, the compositions for topical application may be in the form of a protective care composition for the skin, preferably for the face, the neck, the hands, the feet, or other areas of the body. Non-limiting examples include day creams or lotions, night creams or lotions, sunscreen creams, lotions, or oils, body milks, makeup (a foundation), artificial tanning compositions, depilatories, and patches.
Methods Of Using Natural Plant Materials
In another embodiment, the present invention encompasses a method of improving the condition and aesthetic appearance of skin, comprising applying to an affected area of skin, a composition containing a natural plant material, or extract therefrom, selected from: Plumbago indica; Cananga odorata; Sapindus rarak; Curcuma xanthorrhiza; Physalis minima; or Stephania rotunda, or combinations thereof, or any natural plant material, or extract therefrom, that increases expression levels of any one, or combinations thereof, of genes: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase.
One embodiment of the invention relates to methods of applying an effective amount of a natural plant material, or extract derived therefrom, to induce expression of at least one gene associated with the epidermal-dermal junction, communication between the dermis and epidermis, mechanical properties of the skin, cell-cell cohesion in the epidermis, anchoring of the cells, cell-cell communication, and tissue stability, to an affected area of the skin. The topical composition is preferably applied once daily and remains on the affected area of the skin, where the affected area of the skin includes, but is not limited to the face, neck, legs and thighs, and overall body.
Another embodiment of the present invention relates to a method of improving the condition and aesthetic appearance of skin and comprises applying to the skin, or introducing via a directed mode of delivery, a composition including one or more natural plant materials, or components derived therefrom, in an amount effective to improve the aesthetic appearance of conditions related to skin, where the natural plant material increases expression of one or more of the following genes: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic acid synthase.
In a specific embodiment, the component is in an extract of one or more of the natural plant materials described in a pharmaceutically, physiologically, cosmetically, and dermatologically-acceptable vehicle, diluent, or carrier, where the composition is topically applied to an affected area of skin and left to remain on the affected area in an amount effective for treatment for the dermatological signs of aging and improving the condition and aesthetic appearance of skin.
As will be appreciated by the practitioner, cosmetic treatments comprising compositions containing natural plant materials, including extracts, components, and/or constituents of the invention may be carried out, for example, by topically applying the cosmetic composition as described herein according to the routine technique for administering such compositions. The topical cosmetic composition preferably is applied once daily for a period of at least one week, but may include a period of about 2, 4, 8, or 12 weeks. The cosmetic composition is preferably applied to the face and neck, but may be applied to any area of skin in need of aesthetic improvement, where the cosmetic composition remains on the affected area of skin, and preferably not removed or rinsed off the skin. Routine and commonly practiced techniques encompass the application of creams, lotions, gels, masks, sera, ointments, patches, makeup, makeup-removing milks, sunscreen compositions, or the like, to the skin. Preferably the cosmetic composition is a topical leave on formulation, where spraying as a form of application is also envisioned.
EXAMPLES
The following examples describe specific aspects of the invention to illustrate the invention and provide a description of the present methods for those skilled in the art. The examples should not be construed as limiting the invention, as the examples merely provide specific methodology useful in the understanding and practice of the invention and its various aspects.
Example 1
A plant extract of the present invention may be extracted from natural raw materials by using the methods of aqueous-organic solvent extraction as is well known in the art. One such extraction process is set forth below.
Preparation of Extract
The following describes a suitable method of preparing a natural plant extract useful for preparing a topical composition of the present invention.
Extraction of Plumbago indica 
An extract was obtained by extracting the roots of the Plumbago indica Linn. plant using an ethanol extraction scheme. Briefly, the roots of the Plumbago indica Linn. were first manually ground into small particle sizes resulting in a powder of about 250 grams per flask (2 flasks). The ground powder was then extracted with 80% ethanol (2×1000 ml per flask). After filtering and vacuum evaporation, the total concentrated extract was lyophilized resulting in an ethanolic extract of 35.50 grams. Tannins were removed resulting in an ethanolic extract of Plumbago indica Linn. of 34.08 grams.
The roots of the Plumbago indica Linn. plant were also extracted using a hexane extraction scheme. Where the roots of the Plumbago indica Linn. plant were first manually ground into small particle sizes resulting in a powder of about 250 grams per flask (2 flasks). The ground powder was then extracted with hexane. After filtering and vacuum evaporation, the total concentrated extract was dried by a high vacuum oven, resulting in a hexane extract of Plumbago indica Linn. of 1.70 grams.
Extraction of Cananga odorata Lamk.
Flowers of the Cananga odorata Lamk. plant were extracted using an ethanol extraction scheme similar to that previously described except that there is no tannin removal step. The ethanolic extract of Cananga odorata Lamk. resulted in 62.22 grams.
Flowers of the Cananga odorata Lamk. plant were also extracted using a hexane extraction scheme similar to that previously described. The hexane extract of Cananga odorata Lamk. resulted in 6.26 grams.
Extraction of Sapindus rarak A.
Fruits of the Sapindus rarak A. plant were extracted using an ethanol extraction scheme similar to the previously described method used in extracting the Cananga odorata Lamk. plant except that the ground powder was extracted with 80% ethanol at 2×2000 ml per flask. The ethanolic extract of Sapindus rarak A. resulted in 218.0 grams.
Fruits of the Sapindus rarak A. plant were also extracted using a hexane extraction scheme similar to that of the Cananga odorata Lamk. plant except that the ground powder was extracted with hexane and dried in a hot air oven. The hexane extract of Sapindus rarak A. resulted in 1.03 grams.
Extraction of Curcuma xanthorrhiza Roxb.
Rhizomes of the Curcuma xanthorrhiza Roxb. plant were extracted using an ethanol extraction scheme similar to that used in extracting the Sapindus rarak A. plant. The ethanolic extract of Curcuma xanthorrhiza Roxb. resulted in 48.14 grams.
Rhizomes of the Curcuma xanthorrhiza Roxb. plant were also extracted using a hexane extraction scheme similar to that used in extracting the Sapindus rarak A. plant. The hexane extract of Curcuma xanthorrhiza Roxb. resulted in 29.58 grams.
Extraction of Stephania rotunda Lour.
Rhizomes of the Stephania rotunda Lour. plant were extracted using an ethanol extraction scheme similar to the previously described method used in extracting the Cananga odorata Lamk. plant. The ethanolic extract of Stephania rotunda Lour. resulted in 48.02 grams.
Rhizomes of the Stephania rotunda Lour. plant were also extracted using a hexane extraction scheme similar to that used in extracting the Cananga odorata Lamk. plant. The hexane extract of the Stephania rotunda Lour. plant resulted in 3.22 grams.
Extraction of Physalis minima Linn.
The entire Physalis minima Linn. plant was extracted using an ethanol extraction scheme. Briefly, the whole Physalis minima Linn. was first manually ground into small particle sizes resulting in a powder of about 250 grams per flask (2 flasks). The ground powder was extracted with 80% ethanol (2×2000 ml per flask) and charcoal treated. After filtering and vacuum evaporation, the total concentrated extract was lyophilized resulting in an ethanolic extract of Physalis minima Linn. of 29.57 grams.
The entire Physalis minima Linn. plant was also extracted using a hexane extraction scheme, where the entire Physalis minima Linn. plant was first manually ground into small particle sizes resulting in a powder of about 250 grams per flask (2 flasks). The ground powder was extracted with hexane and charcoal treated. After filtering and vacuum evaporation, the total concentrated extract was dried by hot air in an oven at 40° C., resulting in a hexane extract of Physalis minima Linn. of 4.89 grams.
Example 2
Cell Culturing
A vial of cryopreserved normal human epidermal keratinocytes (Adult or Neonatal) was removed from liquid N2 and placed in a 37° C. water bath for 1-2 minutes, or until completely thawed. The vial was then removed from the water bath and the excess water was wiped off. The vial was placed into a cell culture hood for sterility. The vial was wiped with 70% ethanol to air dry for 30 seconds. While in the cell culture hood, the cells were removed from the vial and placed into a 50 ml tube. The appropriate growth media (5 ml) was added drop wise while agitating the tube, allowing uniform mixture of the cells and medium while limiting cell death due to osmotic changes caused by freezing media. Medium (25 ml) was added to the 50 ml tube to bring the volume up to 30 ml. Into two 75 cm2 flasks, medium/cell solution (15 ml) was added, or to six 25 cm2 flasks, medium (5 ml) was added. Flasks were recapped and placed horizontally in a 37° C. humidified incubator with 5% CO2 for 24 hours. After 24 hours, the old medium was aspirated and fresh room temperature growth medium (15 ml) was added onto the cells. Prior to adding the fresh growth medium, it was brought up to room temperature using a room temperature water bath without the cover. The flasks containing cells with fresh media were placed back into the 37° C. incubator. Flasks containing the old media was replaced with fresh media repeatedly until the desired confluence (75%) was reached.
Plating and Treatment
When the cells reached the desired confluency, they were removed from the incubator and the media was aspirated off using a vacuum trap. Hank's Buffered Saline Solution (HBSS; 10 ml of 1×; Mediatech, Inc.; Herndon, Va.) was placed into the flask and the entire surface area rinsed. The HBSS was then aspirated. Trypsin (1×; Mediatech, Inc.) was added in a 5 ml volume. The flasks were placed back into the incubator for 8 minutes. The flasks were taken from the incubator and 15 ml of Trypsin Neutralizing Solution (1×; Cascade Biologics; Portland, Oreg.) was added to each flask to neutralize the trypsin. The medium/cell solution was removed from the flasks and placed into a 50 ml polypropylene tube. The cells were spun down using a bench top centrifuge at 1100 rpm for 6 minutes at room temperature. After spinning the cells, they were resuspended in growth medium (Basal Medium: Epilife medium with calcium chloride (Cascade Biologics, Inc.); supplemented with 1% human keratinocyte growth factor (Cascade Biologics); 1% Penicillin/Streptomycin (Mediatech, Inc.). The cells were resuspended by pipetting up and down. Cell culture was then placed into 100 mm tissue culture-treated plates in 10 ml volumes for incubation in a 37° C humidified incubator with 5% CO2 until 75% confluency is reached. When confluency was reached, medium was removed and replaced with fresh growth medium containing the test active at a specific concentration. Each test active was diluted in growth medium from a 10% stock vial containing a vehicle (i.e.,water, ethanol, DMSO) and the test active. Each vehicle was tested along side the test active as a control. The 100 mm tissue culture-treated plates were placed back into the 37° C. humidified incubator with 5% CO2 for varying amount of times in order to establish a time course for actives being tested. The tissue culture plates were collected at 1 hour, 4 hour, 8 hour, 24 hours, and 32 hours. At each time point, the growth medium was aspirated and the cells were washed with 1× Phosphate-buffered saline (PBS). The 1× PBS was removed and the 100 mm tissue culture plates were wrapped in parafilm and stored in −80° C. until needed.
RNA Isolation
Parafilm was removed from the outside of the 100 mm tissue culture plates and 2 ml of Trizol Reagent (1×; Invitrogen, Corp.; Carlsbad, Calif.) was added to the cells. The plate was scraped and the solution was placed into 2-1.7 ml microcentrifuge tubes. Chloroform (250 ml) was added to each tube and vortexed for 15-30 seconds. Each tube was incubated at room temperature (25° C.) for 3-5 minutes and centrifuged at 12,000 rpm at 4° C. for 10 minutes. The top layer of the solution was removed without disturbing the two bottom layers and placed into a separate 1.7 ml microcentrifuge tube. Ice cold isopropanol (1.0 ml) was added to the tube and incubated on ice for 10-15 minutes. The sample was centrifuged for 15 minutes at 4° C. and at 12,000 rpm. Isopropanol was poured off and ice cold 70% ethanol (1 ml) was added to the tube in order to remove the salt precipitate on the RNA sample. The sample was centrifuged for 10 minutes at 4° C. and at 12,000 rpm. The 70% ethanol was poured off and ice cold 100% ethanol was added. The sample was centrifuged for 10 minutes at 4° C. and at 12,000 rpm. The tube was dried by inverting and removing all of the 100% ethanol from the tube. The tube was stored in −80° C., either diluted in RNase-free water or dried.
Example 3
PCR Gene Expression using Plant Extracts
Gene expression levels of six different genes associated with dermatological signs of aging, specifically, Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, and Hyaluronic Acid Synthase, were shown to increase in the presence of one or more of the following plants: Plumbago indica, Cananga odorata, Sapindus rarak, Curcuma xanthorrhizza, Physalis minima, and Stephania rotunda. The polymerase chain reaction (PCR) assay was performed as is commonly practiced in the art (See, e.g., J. Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.). Specific thermocycle parameters used were as follows: 1 cycle, 1 minute, at 95° C.; 40 cycles, 12 seconds, at 95° C.; and 1 cycle, 30 seconds, at 60° C. in a volume of 50 microliters.











TABLE 1









β-Catenin
Collagen 4
Collagen 7














Transcript #
p
Transcript #
p
Transcript #
p

















Plumbago
12.51820555
0.01
11.81759611
6.28 × 10−5
14.09093
7.89 × 10−4


indica (E)
9.736382094

11.17880713

12.96366



9.272744852



12.96366


Plumbago
10.0111709
0.004
9.706302
0.0027
8.113628
3.07 × 10−4


indica (H)
9.269602689

6.130296

7.662871



8.157250366

7.918299

8.113628


Cananga
8.961288
0.01
12.34666
0.001
10.89411
 4.1 × 10−4


odorata (E)
7.46774

8.917033

10.89411



6.47204133

13.03259

11.49934


Cananga
11.72896598
0.002
10.66554
1.98 × 10−5
17.68084
0.002


odorata (H)
11.72896598

11.63513

17.11049



10.32149006

10.34234

15.39944


Sapindus
1.602004396
0.024
—
—
—
—


rarak (E)
1.602004396



1.127336427


Sapindus
—
—
—
—
—
—


rarak (H)


Curcuma
1.676812229
0.001
—
—
2.664477
0.015


xanthorrhizza
1.64739447



2.910429


(E)
1.559141195



2.254558


Curcuma
1.269907935
0.022
—
—
1.732678
0.00136


xanthorrhizza
1.455102842



1.769544


(H)
1.190538689



1.732678


Physalis
1.29537639
0.024
—
—
—
—


minima (E)
1.250707514



1.161371263


Physalis
—
—
—
—
—
—


minima (H)


Stephania
—
—
—
—
1.322025
0.04


rotunda (E)




1.092108







1.149587


Stephania
1.511618817
0.009
—
—
2.041838
 5.9 × 10−4


rotunda (H)
1.455632935



1.905715



1.287675289



1.905715





Only statistically significant numbers shown. Transcript numbers are normalized to vehicle control. Indicated p value from t test with vehicle control.


(E): Ethanol extract


(H): Hexane extract


(—): Not statistically significant


p < 0.05: Statistically significant

















TABLE 2











Hyaluronic acid



Frizzled 10
Estrogen receptor α
synthase














Transcript #
p
Transcript #
p
Transcript #
p

















Plumbago
12.91926
0.0061
10.60385
3.9 × 10−4
16.45067
0.008


indica (E)
10.33541

9.766702

12.04104



13.24224

10.04575

13.8532


Plumbago
5.553399
0.006
7.141224
0.004
11.35577
0.007


indica (H)
6.199143

5.802245

9.416984



7.103184

6.91806

9.278498


Cananga
8.843769
 2.61 × 10−4
9.288834
0.013
8.18149
0.016


odorata (E)
9.190583

11.68595

9.297147



9.190583

13.18415

6.32206


Cananga
10.78532
0.004
11.85962
0.011
12.96681
0.009


odorata (H)
9.64142

12.42436

11.56499



8.987765

16.09519

9.812723


Sapindus
23.06456
0.0025
—
—
—
—


rarak (E)
21.29036



19.51616


Sapindus
7.256429
 9.7 × 10−4
3.645494
0.023
—
—


rarak (H)
7.709956

3.410301



7.029666

2.783119


Curcuma
228.9185
6.99 × 10−4
—
—
—
—


xanthorrhizza
228.9185


(E)
211.3093


Curcuma
52.26041
0.005
—
—
—
—


xanthorrhizza
44.34217


(H)
41.96669


Physalis
—
—
—
—
—
—


minima (E)


Physalis
1.444326
0.022
—
—
1.135447
0.002


minima (H)
1.444326



1.173295



1.444326



1.324688


Stephania
—
—
—
—
—
—


rotunda (E)


Stephania
1.950059
0.013
—
—
—
—


rotunda (H)
2.145065



1.930559





Only statistically significant numbers shown. Transcript numbers are normalized to vehicle control. Indicated p value from t test with vehicle control.


(E): Ethanol extract


(H): Hexane extract


(—): Not statistically significant


p < 0.05: Statistically significant






The content of all patents, patent applications, published articles, abstracts, books, reference manuals and abstracts, as cited herein are hereby incorporated by reference in their entireties to more fully describe the state of the art to which the invention pertains.
It should be understood that the foregoing description is only illustrative of the present invention. Various alternatives and modifications can be devised by those skilled in the art without departing from the invention. Accordingly, the present invention is intended to embrace all such alternatives, modifications and variations that fall within the scope of the appended claims.

## Claims

1. A method of ameliorating, reducing, or treating progressive degradation of a dermal-epidermal junction and/or degradation of a cell-cell cohesion in skin, comprising topically applying to skin a composition, comprising:
(a) a cosmetically, dermatologically, pharmaceutically, or physiologically effective amount at least one plant extract, or any combinations thereof, sufficient to increase expression levels of at least one gene selected from the group consisting of: Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, Hyaluronic acid synthase, and combinations thereof, wherein said at least one plant extract is Plumbago indica, Sapindus rarak, or Stephania rotunda, either alone or in combination with each other and/or with Cananga odorata; and
(b) a cosmetically, dermatologically, pharmaceutically, or physiologically acceptable vehicle,

in an amount effective to ameliorate, reduce, or treat progressive degradation of a dermal-epidermal junction and/or degradation of a cell-cell cohesion in skin.

2. The method of claim 1, wherein said composition is applied for a period of time sufficient to ameliorate, reduce, or treat progressive degradation of a dermal-epidermal junction and/or degradation of a cell-cell cohesion in skin.

3. The method of claim 1, wherein said applying of the composition occurs at least once daily for a period of time sufficient to ameliorate, reduce, or treat progressive degradation of a dermal-epidermal junction and/or degradation of a cell-cell cohesion in skin.

4. The method of claim 1, wherein the plant extract is present in an amount effective to improve the condition and/or aesthetic appearance of skin.

5. The method of claim 1, wherein the extract is present in an amount from about 0.0001 weight % to about 50 weight %, based upon the total weight of the composition.

6. The method of claim 1, wherein the extract is present in an amount from about 0.001 weight % to about 20 weight %, based upon the total weight of the composition.

7. The method of claim 4, wherein the improvement in aesthetic appearance is selected from the group consisting of: reducing dermatological signs of chronological aging, photo-aging, hormonal aging, and/or actinic aging; reducing the appearance of lines and/or wrinkles; reducing the noticeability of facial lines and wrinkles, facial wrinkles on the cheeks, forehead, perpendicular wrinkles between the eyes, horizontal wrinkles above the eyes, and around the mouth, marionette lines, and particularly deep wrinkles or creases; reducing and/or diminishing the appearance and/or depth of lines and/or wrinkles; improving the appearance of suborbital lines and/or periorbital lines; reducing the appearance of crow's feet; rejuvenating and/or revitalizing skin, particularly aging skin; reducing skin fragility; reversing of loss of glycosaminoglycans and/or collagen; ameliorating the effects of estrogen imbalance; reducing skin atrophy; reducing and/or treating hyperpigmentation, minimizing skin discoloration; improving skin tone, radiance, clarity and/or tautness; reducing and/or ameliorating skin sagging; improving skin firmness, plumpness, suppleness and/or softness; improving procollagen and/or collagen production; improving skin texture and/or promoting retexturization; improving skin barrier repair and/or function; improving the appearance of skin contours; restoring skin luster and/or brightness; minimizing dermatological signs of fatigue and/or stress; resisting environmental stress; replenishing ingredients in the skin decreased by aging and/or menopause; improving communication among skin cells; increasing cell proliferation and/or multiplication; increasing skin cell metabolism decreased by aging and/or menopause; retarding cellular. aging; improving skin moisturization; enhancing skin thickness; increasing skin elasticity and/or resiliency; enhancing exfoliation; improving microcirculation; decreasing cellulite formation; and any combinations thereof.

8. A method of improving the condition and/or aesthetic appearance of skin comprising topically applying to skin a composition, comprising:
(a) a cosmetically, dermatologically, pharmaceutically, or physiologically effective amount at least one plant extract, or any combinations thereof, sufficient to increase expression levels of at least one gene selected from the group consisting of; Beta-catenin, Collagen 4, Collagen 7, Frizzled 10, Estrogen Receptor alpha, Hyaluronic acid synthase, and combinations thereof; wherein said at least one plant extract is Plumbago indica, Sapindus rarak, or Stephania rotunda, either alone or in combination with each other and/or with Cananga odorata; and
(b) a cosmetically, dermatologically, pharmaceutically, or physiologically acceptable vehicle,

in an amount effective to improve the condition and/or aesthetic appearance of skin.

9. The method of claim 8, wherein said composition is applied for a period of time sufficient to improve the aesthetic appearance of skin.

10. The method of claim 8, wherein said applying of the composition occurs at least once daily for a period of time sufficient to improve the aesthetic appearance of skin.

11. The method of claim 8, wherein the plant extract is present in an amount from about 0.0001 weight % to about 50 weight %, based upon the total weight of the composition.

12. The method of claim 8, wherein the plant extract is present in an amount from about 0.001 weight % to about 20 weight %, based upon the total weight of the composition.

13. The method of claim 8, wherein the improvement in aesthetic appearance is selected from the group consisting of: reducing dermatological signs of chronological aging, photo-aging, hormonal aging, and/or actinic aging; reducing the appearance of lines and/or wrinkles; reducing the noticeability of facial lines and wrinkles, facial wrinkles on the cheeks, forehead, perpendicular wrinkles between the eyes, horizontal wrinkles above the eyes, and around the mouth, marionette lines, and particularly deep wrinkles or creases; reducing and/or diminishing the appearance and/or depth of lines and/or wrinkles; improving the appearance of suborbital lines and/or periorbital lines; reducing the appearance of crow's feet; rejuvenating and/or revitalizing skin, particularly aging skin; reducing skin fragility; reversing of loss of glycosaminoglycans and/or collagen; ameliorating the effects of estrogen imbalance; reducing skin atrophy; reducing and/or treating hyperpigmentation; minimizing skin discoloration; improving skin tone, radiance, clarity and/or tautness; reducing and/or ameliorating skin sagging; improving skin firmness, plumpness, suppleness and/or softness; improving procollagen and/or collagen production; improving skin texture and/or promoting retexturization; improving skin barrier repair and/or function; improving the appearance of skin contours; restoring skin luster and/or brightness; minimizing dermatological signs of fatigue an d/or stress; resisting environmental stress; replenishing ingredients in the skin decreased by aging and/or menopause; improving communication among skin cells; increasing cell proliferation and/or multiplication;
increasing skin cell metabolism decreased by aging and/or menopause; retarding cellular aging; improving skin moisturization; enhancing skin thickness; increasing skin elasticity and/or resiliency; enhancing exfoliation; improving microcirculation; decreasing cellulite formation; and any combinations thereof.

14. The method of claim 1 wherein the gene is Beta-catenin.

15. The method of claim 1 wherein the gene is Collagen 4.

16. The method of claim 1 wherein the gene is Collagen 7.

17. The method of claim 1 wherein the gene is Frizzled 10.

18. The method of claim 1 wherein the gene is Estrogen Receptor alpha.

19. The method of claim 1 wherein the gene is Hyaluronic acid syntase.

20. The method of claim 1 wherein the plant extract is Plumbago indica.

21. The method of claim 1 wherein the plant extract is Sapindus rarak.

22. The method of claim 1 wherein the plant extract is Stephania rotunda.

23. The method of claim 8 wherein the gene is Beta-catenin.

24. The method of claim 8 wherein the gene is Collagen 4.

25. The method of claim 8 wherein the gene is Collagen 7.

26. The method of claim 8 wherein the gene is Frizzled 10.

27. The method of claim 8 wherein the gene is Estrogen Receptor alpha.

28. The method of claim 8 wherein the gene is Hyaluronic acid syntase.

29. The method of claim 8 wherein the plant extract is Plumbago indica.

30. The method of claim 8 wherein the plant extract is Sapindus rarak.

31. The method of claim 8 wherein the plant extract is Stephania rotunda