# Patent

## Title
Process for the stabilization of proteins in an aqueous solution comprising cysteine in a concentration between 150 and 220mM

## Abstract

The present invention relates to a process for the storage of proteins in an aqueous solution. The addition of cysteine delays the temporal decrease in the effective concentration of the protein. The process is suitable for use in the production of heterologous proteins in microorganisms.

## Background
This application is a continuation of application Ser. No. 09/991,964, filed Nov. 26, 2001, now U.S. Pat. No. 6,734,164, issued May 11, 2004; which is a continuation of application Ser. No. 09/608,297, filed Jun. 30, 2000, now U.S. Pat. No. 6,339,061 B1, issued Jan. 15, 2002. This application also claims priority to German Application No 19930676.1, filed Jul. 2, 1999. All of the above applications', in their entirety, are incorporated by reference herein.


FIELD OF THE INVENTION
The present invention relates to a process for the storage of proteins in an aqueous solvent.
BACKGROUND OF THE INVENTION
The chemical state of the SH groups of proteins or other oxidation-sensitive structures often has an effect on the identity, activity, or effective concentration of proteins. Identity means the respective folding of a protein. Activity should be understood as meaning enzyme activity. The effective concentration of a protein should be the proportion of the protein in a solution, which is correctly folded with respect to the biological in vivo function.
It is known from the literature that structure-modifying oxidations of proteins can be suppressed by the use of thiol reagents such as, for example, 2-mercaptoethanol or cysteine.
For example, D-amino acid oxidase (DAO) can be stabilized by thiols. The flavoprotein DAO catalyzes the stereospecific deamination of D-amino acids to the corresponding α-ketoacids and ammonium (P. Golini et al., Enzyme and Microbial Technology 17:324-329 (1995)). However, the addition of thiols such as, for example, cysteine can also reduce the activity of proteins. This effect can also be explained by the presence of cysteine radicals. An example of this is aminoacylase. Aminoacylase is a dimeric enzyme having one Zn2+ atom per subunit. Each subunit of the enzyme contains 2 cysteine SH groups and 2 disulfide bonds. The chemical modification of the SH groups, such as the breaking of the disulfide bonds, can lead to an inactivation of the enzyme. It was possible to show that, by the addition of 2-mercaptoethanol, the activity of the aminoacylase is reduced, whereas after removal of the 2-mercaptoethanol by dialysis or gel filtration the original enzyme activity can be almost completely restored (W. Kördel and F. Schneider, Biochem. Biophys. Acta 445:446-457 (1976)).
For cysteine and some derivatives, it was possible for certain preparation forms and specific applications to demonstrate an antibacterial, antiviral or antifungal activity to a certain extent. Thus, for example, it was possible to show that the addition of cysteine is suitable to a certain extent as protection against the spoilage of foods (U.S. Pat. No. 4,937,085).
With the aid of genetic engineering processes, it is possible to synthesize recombinant proteins, such as insulin or its precursors, and also insulin derivatives which have amino acid compositions differing from the derived gene sequence (e.g., human), in genetically modified microorganisms, such as the bacterium Escherichia coli. 
Recombinant synthesis in microorganisms is carried out with the aid of expression vectors. These expression vectors consist of a vector plasmid containing a control sequence for the replication of the plasmid and a selection gene (inter alia, antibiotic resistance gene, metabolic marker). The coding region of the gene for the protein of interest (e.g., insulin) may be inserted under the control of a promoter that is active in the chosen microorganism. For example, if one chooses E. coli, the lac promoter may be used to control expression of the protein of interest.
A process for the production of recombinant proteins (e.g., insulin or insulin derivatives) with the cooperation of genetically modified microorganisms is composed of a series of process steps, which intermesh with each other and must be coordinated with one another.
Thus, for example, a process for the production of human insulin in E. coli can be constructed from the following process steps:
Fermentation of the microorganisms; cell separation; cell disruption; isolation and intermediate storage of the fusion protein with cysteine; refolding into the native spatial structure including formation of the correct disulfide bridges and subsequent separation of foreign proteins not containing material of value; enzymatic cleavage to the arginylinsulin; basic purification of the aqueous protein solution; 1st chromatographic purification; enzymatic cleavage to human insulin; 2nd chromatographic purification; high purification by means of HPLC; recrystallization; and drying.
The large number of individual steps carried out, as a rule, leads to a considerable loss in total yield, because losses in the specific yield of each individual process step are unavoidable.
By optimizing these intermediate steps, the total yield can be improved. There is considerable interest in such processes in order to improve economical utilization of the resources employed and to decrease environmental pollution.
For example, EP 0906918 describes an improved process for the production of a precursor of insulin or insulin derivatives having correctly linked cystine bridges in the presence of cysteine or cysteine hydrochloride and of a chaotropic auxiliary.
Insulin derivatives are derivatives of naturally occurring insulins, such as human insulin or animal insulins. These insulin derivatives differ from the naturally occurring insulin by the deletion, substitution, and/or addition of at least one genetically encodable amino acid residue in the naturally occurring insulin.
During the storage of proteins, a decrease in the effective concentration usually occurs.
The storage of production products between individual process steps may be necessary for various reasons. For example, a subsequent industrial processing step may not be able to accept the total amount of product of the preceding process step, thus requiring storage of a portion of the previous product.
The time needed for intermediate storage may be of differing length. Owing to capacity, the need to coordinate industrial units, the required delivery of more chemicals or appliances, or other reasons, it may be necessary to extend the intermediate storage to several weeks.
One effective way of increasing the yield of the final product is to reduce the loss of active protein that may occur during the unavoidable intermediate storage of products from individual process steps before further processing takes place.
The use of cysteine or its derivatives for the control of the potential loss in yield of active protein during intermediate storage of production products has not been described until how. These products can differ in composition, as well as in form, including biological components, for example, with participation of enzyme catalysts or genetically modified microorganisms. Such processes are used, for example, in the preparation of insulin.
Depending on the process step, the production products to be intermediately stored can consist of, inter alia, different amounts of complex macromolecules of a biological nature (e.g., proteins, DNA, fats), microorganisms, buffer substances, and starting materials.
The production process, as a rule, is aimed at the preparation of a substance that is as uniform as possible, e.g., the production of insulin from a genetically modified microorganism. If intermediate products have to be stored, e.g., in the preparation of insulin, loss of effective concentration of this protein regularly occurs.
Storage of a protein should be understood as meaning any storage of the protein, regardless of the volumetric amount in which the protein is present, the time period of the storage, or the temperature conditions under which the storage takes place. The storage of proteins normally takes place in aqueous solutions.
An aqueous solution of a protein may contain constituents of nutrient media for the culture of microorganisms in defined form or as a complete media containing, in particular, carbon sources or nitrogen sources, amino acids, inorganic salts, and trace elements. The aqueous solution may also contain buffer components of different chemical buffer types, as well as macromolecules of biological origin, such as DNA or fats. The aqueous solution may additionally contain organic or inorganic compounds such as, for example, sodium dodecylsulfate (“SDS”) or potassium acetate, and also proportions of solvents of differing priority, such as methanol or petroleum ether.
SUMMARY OF THE INVENTION
The present invention relates to a process for the storage of a protein in an aqueous solution, which comprises delaying the temporal decrease in the effective concentration of the protein by the addition of cysteine to the aqueous solution.
The process is suitable for the stabilization of proteins during storage thereof. The addition of cysteine slows the temporal decrease in the effective concentration of a protein during its storage in aqueous solutions. The process can be used, for example, in the production of proteins by microorganisms. Examples of appropriate microorganisms are, inter alia, preferably bacteria, particularly Escherichia coli; yeasts, particularly Saccharomyces cerevisiae or Pichia pastoris. In addition, the production of proteins may be by insect cells. In the preferred embodiment, these microorganisms can be transformed using expression vector constructs for the induced or constitutive expression of said protein. The microorganisms are cultured and then disrupted after synthesis of the protein. Suitable disruption methods include all processes that are suitable for the release of the protein from the microorganism, for example, ultrasound, chemical methods using potassium acetate, SDS, or lysozyme, heating of the microorganisms, or the French press. The disruption of the microorganisms may be omitted if the protein to be prepared is secreted directly into the medium. In principle, the process is also applicable to extruded proteins.
The complex protein mixtures, which result when the microorganisms are disrupted or when the protein is extruded directly into the medium, are usually in aqueous solution. The proteins may be dissolved therein or present in suspended form. These protein solutions are preferably stored from about 0° C. to about 50° C. or from about 5° C. to about 30° C. or at about 5° C. To delay the inactivation of the protein of interest, cysteine is added to this protein mixture. The concentration of cysteine in the protein mixture may be between 100 and 500 mM or preferably between 150 and 220 mM. A concentration of 170 mM is more preferable. In this way, proteins in complex molecule solutions can be stored for up to several weeks with only a slight decrease in the effective protein concentration. The process is applicable to the synthesis of heterologous proteins, in particular in microorganisms, after their disruption with subsequent purification and possible renaturation, preferably for the preparation of insulin and its precursor.
Proteins used for storage in a process of the invention are preferably insulin or insulin derivatives.
The present invention further relates to a process for the preparation of a heterologous protein, comprising the expression of the heterologous protein or its precursor in a transformed microorganism. The heterologous protein is then stored by means of a process according to the present invention. This heterologous protein or a precursor thereof is optionally then renatured, purified of leader sequences or otherwise processed, and finally prepared to give the desired product after purification and isolation. Suitable heterologous proteins are preferably insulin or insulin derivatives, or precursors of insulin.


DETAILED DESCRIPTION OF THE INVENTION
During the implementation of the processes for the preparation of insulin, it was found that, by adding cysteine to the fusion protein, the product remained stable in storage for months. In comparison, the untreated product lost some of its activity irreversibly after just a few days.
The precursor molecules of human insulin or an insulin derivative were used in the following examples. The structure and amino acid sequence were disclosed in EP 906 918. The precursor of human insulin has the sequence of naturally occurring human insulin. The precursor of the insulin derivative contains a glycine instead of an arginine at position 21 of the A chain and two arginine molecules at positions 31 and 32 at the C-terminal end of the B chain.
Fusion proteins of the insulins can be prepared by fermentation of genetically modified E. coli cells according to EP 0 489 780 and EP 0 906 918. A protein suspension was obtained containing approximately 20 to 25% dry matter and 40 to 50% foldable insulin.
For the sought-after stabilization of the protein by means of cysteine, 75 kg of cysteine hydrochloride×H2O was introduced into about 2500 kg of this protein suspension (corresponding to one fermentation batch) over the course of 20 minutes with vigorous stirring. The pH fell from about 7.0 to about 2.5 during this process. The suspension became very pasty in the pH range around 5 and readily stirrable again from around pH 4. Under these experimental conditions, a cysteine hydrochloride concentration of about 170 mM was established in the protein suspension. The protein suspension was subsequently stirred for about 60 min., after which it remained without further stirring until working-up.
EXAMPLE 1
For experimental confirmation of the preserving action of cysteine, the following laboratory experiments were carried out:
A fusion protein of human insulin and a fusion protein of the insulin derivative prepared according to EP 906 918 were stored with and without cysteine at 5° C. and at room temperature for up to 2 months. During the experiment, aliquots were periodically removed from the batches. The fusion protein was converted into the prepro form of human insulin or prepro form of the insulin derivative by reductive folding (EP 906 918). The amount of cysteine necessary for folding was added to each batch that had been stored without cysteine hydrochloride monohydrate about 1 hour before the start of folding. In those batches containing cysteine in the form of the preservative, it was not necessary to add additional cysteine to obtain proper folding of the insulin molecule.
The prepro form of human insulin or prepro form of the insulin derivative was determined by means of HPLC.
HPLC analysis:
0.5 g of protein was dissolved in 40 ml of a solution containing 6 M guanidine hydrochloride, 50 mM TRIS, 5 mM ethylenediaminetetraacetate (EDTA) at pH 8.5, 1% 2-mercaptoethanol, and 10 mM dithiothreitol at 95° C. for 2 min and then centrifuged at 14,000 g for 20 min. 0.02 ml of the clear supernatant was applied to a high-pressure liquid chromatography column.

    Column: Nucleogel® RP 300-5/46 (Macherey & Nagel, Aachen, Germany)
    Gradient: buffer A: 0.1% trifluoroacetic acid (TFA)
    
        buffer B: 0.09% TFA in acetonitrile
    
    
    Temperature: 55° C.
    Total running time: 40 min.


The gradient was defined by the following amounts of buffer B after the corresponding running times: 10 min 25%, 12 min 60%, 13 min 90%, 15 min 100%.

    Flow: 1 ml/min
    Detection: 215 nm

Result of the Experiments










TABLE 1







Storage of fusion protein of human insulin at 5° C., valuable substance


(mg/l) after folding (valuable substance means folded human insulin)













1st day
1 week
2 weeks
4 weeks
8 weeks
















With cysteine
850
831
867
845
825


Without cysteine
879
827
790
712
625
















TABLE 2







Storage of fusion protein of human insulin at room temperature,


valuable substance (mg/l) after folding













1st day
1 week
2 weeks
4 weeks
8 weeks
















With cysteine
792
817
800
785
790


Without cysteine
812
654
312
%*
%*





*Application not possible because of severe putrefaction processes













TABLE 3







Storage of fusion protein of the insulin derivative at 5° C.,


valuable substance (mg/l) after folding













1st day
1 week
2 weeks
4 weeks
8 weeks
















With cysteine
590
553
540
573
552


Without cysteine
612
580
549
518
404
















TABLE 4







Storage of fusion protein of the insulin derivative at room


temperature, valuable substance (mg/l) after folding













1st day
1 week
2 weeks
4 weeks
8 weeks
















With cysteine
643
667
680
649
653


Without cysteine
597
547
420
271
%*





*Application not possible because of severe putrefaction processes






The addition of cysteine or cysteine hydrochloride allowed storage times of up to 2 months without significant loss of activity.

## Claims

1. A process for the storage of a protein in an aqueous solution, comprising adding an amount of cysteine to make the concentration of cysteine between 150 and 220 mM thereby delaying decrease in the effective concentration of the protein by reducing chemical modification of SH groups on the protein during storage.

2. The process as claimed in claim 1, wherein the effective concentration does not decrease by more than about 3%.

3. The process as claimed in claim 1, wherein the protein is a heterologous protein produced in an organism.

4. The process as claimed in claim 3, wherein the organism is a microorganism.

5. The process as claimed in claim 4, wherein the microorganism is a bacterium.

6. The process as claimed in 5, wherein the bacterium is Escherichia coli.

7. The process as claimed in claim 4, wherein the microorganism is a yeast.

8. The process as claimed in claim 7, wherein the yeast is Saccharomyces cerevisiae.

9. The process as claimed in claim 7, wherein the yeast is Pichia pastoris.

10. The process as claimed in claim 1, wherein the protein is a heterologous protein and is produced in an insect cell.

11. The process as claimed in claim 3 or claim 10, wherein the protein is encoded by an expression vector comprising a nucleic acid encoding the protein.

12. The process as claimed in claim 1, wherein the protein is present in dissolved form.

13. The process as claimed in claim 1, wherein the protein is present in suspension.

14. The process as claimed in claim 1, wherein the storage of the protein takes place at about 0° C. to about 50° C.

15. The process as claimed in claim 14, wherein the storage of the protein takes place at about 5° C. to about 30° C.

16. The process as claimed in claim 14, wherein the storage of the protein takes place at about 5° C.

17. The process as claimed in claim 1, wherein the protein is insulin, an insulin derivative, or a precursor thereof.

18. A process for the preparation and storage of a heterologous protein, comprising the expression of the heterologous protein or a precursor thereof in a transformed microorganism, optional disruption of the microorganism and/or isolation of the heterologous protein or its precursor from the culture medium, and the subsequent storage of the heterologous protein according to the process of claim 1.

19. The process of claim 18, wherein the protein has been denatured, said process comprising renaturation of the heterologous protein or its precursor and the purification of the heterologous protein, including optional removal of a leader sequence or other sequences that may be present in the precursor of the heterologous protein.

20. The process as claimed in claim 18, wherein the heterologous protein is animal insulin.

21. The process as claimed in claim 20, wherein the animal insulin is human insulin.

22. A process for the storage of a protein in an aqueous solution, comprising adding an amount of cysteine to make the concentration of cysteine between 150 and 220 mM thereby delaying decrease in the effective concentration of the protein by reducing chemical modification of SH groups on the protein during a period of greater than 24 hours.

23. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a storage period of 48 hours or more.

24. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a storage period of 1 week or more.

25. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a storage period of 2 weeks or more.

26. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a storage period of 4 weeks or more.

27. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a period of 8 weeks or more.

28. The process as claimed in claim 22, wherein the temporal decrease in the effective concentration of the protein is delayed for a period of from greater than 24 hours to 2 months.

29. The process as claimed in claim 22, wherein the protein is a heterologous protein produced in an organism.

30. The process as claimed in claim 29, wherein the organism is a microorganism.

31. The process as claimed in claim 30, wherein the microorganism is a bacterium.

32. The process as claimed in claim 31, wherein the bacterium is Escherichia coli.

33. The process as claimed in claim 30, wherein the microorganism is a yeast.

34. The process as claimed in claim 33, wherein the yeast is Saccharomyces cerevisiae.

35. The process as claimed in claim 33, wherein the yeast is Pichia Pastoris.

36. The process as claimed in claim 22, wherein the protein is a heterologous protein and is produced in an insect cell.

37. The process as claimed in claim 29 or claim 36. wherein the protein is encoded by an expression vector comprising a nucleic acid encoding the protein.

38. The process as claimed in claim 22, wherein the protein is present in dissolved form.

39. The process as claimed in claim 22, wherein the protein is present in suspension.

40. The process as claimed in claim 22, wherein the storage of the protein takes place at about 0° C. to about 50° C.

41. The process as claimed in claim 40, wherein the storage of the protein takes place at about 5° C. to about 30° C.

42. The process as claimed in claim 40, wherein the storage of the protein takes place at about 5° C.

43. The process as claimed in claim 22, wherein the protein stored is insulin, an insulin derivative, or a precursor thereof.

44. A process for the preparation and storage of a heterologous protein, comprising the expression of the heterologous protein or its precursor in a transformed microorganism, optional disruption of the microorganism and/or isolation of the heterologous protein or its precursor from the culture medium, and the subsequent storage of the heterologous protein according to the process of claim 22.

45. The process of claim 44, wherein the protein has been denatured, said process comprising renaturation of the heterologous protein or its precursor and the purification of the heterologous protein, including optional removal of a leader sequence or other sequences that may be present in the precursor of the heterologous protein.

46. The process as claimed in claim 44, wherein the heterologous protein is animal insulin.

47. The process as claimed in claim 44, wherein the animal insulin is human insulin.