Dr. Bruce Crise

Director, Business Development

Scientific and Technical Operations

Advanced Technology Partnerships Initiative

SAIC-Frederick/NCI-Frederick

The Advanced Technology 

Partnerships Initiative: Access 

to World-Class Bio-Imaging 

Resources Through 

Collaboration 

10/06/2009 U.S. Department of Health and Human Services

National Institutes of Health
Advanced Technology Partnerships 

Initiative

•The Mission...

•To accelerate new discoveries, 

technologies, diagnostics and 

therapeutics to cancer and AIDS 

patients—by leveraging our 

unique combination of resources 

and advanced technologies, and 

through effective strategic 

partnerships.
ATPI Technologies, Capabilities, and 

Expertise

Biopharmaceutical

Development

Genetics and

Genomics

Proteins and 

Proteomics

Advanced

Biomedical Computing

Advanced

Technology Partnerships

Initiative

Imaging and

Nanotechnology

Integrated

In Vivo Services

Center for Applied 

Preclinical Research (CAPR)
Imaging and Nanotechnology Group (ING)

Optical Microscopy Analysis Lab (OMAL)

Stephen Lockett

Electron Microscopy Lab (EML)

Kunio Nagashima

Nanotechnology Characterization Lab (NCL)

Scott McNeil

U.S. Department of Health and Human Services

National Institutes of Health
Optical Microscopy Analysis Lab (OMAL)

• Live cell imaging

• Highly efficient software for 

segmentation and delineation 

of cells and nuclei in intact 

tissue

• Four state-of-the-art confocal 

microscopes

• Fluorescence resonance energy 

transfer (FRET) 

– to determine intracellular 

locations of interacting pairs

• Fluorescence recovery after 

photobleaching (FRAP)

– to assess cell membrane 

diffusion and protein binding 

• Photo-activated localization 

microscopy (PALM)

U.S. Department of Health and Human Services

National Institutes of Health
Transmission Electron 

Microscope (TEM),

Immuno-Electron 

Microscopy (IEM)

Scanning Electron 

Microscope (SEM)

Immuno-markers, Post-embedding IEM

Blood cells

Electron Microscopy Lab (EML)

Cryo-TEM

3D Tomography

Scanning Transmission 

Electron Microscopy 

(STEM)/EDS

U.S. Department of Health and Human Services

National Institutes of Health
Translational Support to Nanotechnology:

Nanotechnology Characterization Lab (NCL)

• EM coupled EDX

• Atomic Force Microscopy (AFM)

• TEM

• Cryo-EM

• PET/CT

• MRI

• ICP-MS

• FFF

• DLS/ ZetaSizer

• SEC MALLS

NCL Objectives

• Characterize nanoparticles using standardized methods

• Conduct structure-activity relationship studies

• Facilitate regulatory review of nanotech constructs

• Engage in educational and knowledge-sharing efforts

NCL provides infrastructure support to the Alliance and to nanotech 

researchers to overcome obstacles and translate ‘nano’ into the clinic.

NCL Support Capabilities:

U.S. Department of Health and Human Services

National Institutes of Health
Advanced Technology Research Facility 

and Research Park

Future Space: Accelerating R&D by bringing partners together

World-Class Design for Co-located R&D Programs

 ATRF = 330,000 feet2

 ATRP = 140 acres

 Beta testing, collaboration, & training space

 ATP, BDP, and CCR laboratories

 Frederick, Maryland

U.S. Department of Health and Human Services

National Institutes of Health
Working with SAIC-F FFRDC

A Research Institute for Support

 Support SBIR grant applications (e.g. LOI)

 Consultation

 Contracting and Business Mechanisms

• Interagency Agreements

• CRADAs

• MTAs

• Beta Testing

• Collaborative Agreements

• Training, Guest Researcher Agreements

U.S. Department of Health and Human Services

National Institutes of Health
Advanced Technology Partnerships Initiative

http://www.ncifcrf.gov/atpi/

U.S. Department of Health and Human Services

National Institutes of Health