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The 2022 NIST-Hosted Workshop on Standards for 

Microbiome and Multi'Omics Measurements

AGENDA

Updated 08/09/2022

Day 1: Tuesday, Aug 23, 2022 (all times in MT)

8:15 – 8:45 AM WELCOME Breakfast & INTRODUCTORY REMARKS

Welcome, Logistics, NIST & Workshop Overview –

Dr. Scott Jackson, Leader of the Complex Microbial Systems Group, 

Material Measurement Laboratory at the National Institute of Standards 

and Technology 

8:45 – 10:15 AM SESSION 1 MODERATOR: SCOTT JACKSON (NIST)

8:45 AM V-Establishing the 1st WHO International Reference Reagents for 

microbiome analysis (Chrysi Sergaki)

9:10 AM V-Non-Viable bacteria in Microbiome Analyses can Skew Taxonomic 

Results (Jason Arnold)

9:22 AM IP-Minimum Requirements for Producing High Performance Whole Cell 

Microbial Reference Standards for DNA and RNA Applications (Scott 

Tighe)

9:34 AM IP-Characterization, Hi-Fi Genome Assembly, and Data Sharing of a 

True Diversity Microbiome Reference (Raul Cano)

9:46 AM IP-NIST Stool Reference Material for Multi’omic Analyses (Stephanie 

Servetas)

10:35

10:15 – 10:35 AM Morning BREAK

10:35 – 12:05 PM SESSION 2 MODERATOR: CLAY DAVIS (NIST)

10:35 AM V-Linking individual microbial and metabolite biomarkers of Colorectal 

Cancer using computational models of the gut microbiota metabolism 

(Ali R. Zomorrodi)

10:45 AM IP-NIST Calibrant Solution for Fecal Metabolomics (Sandra Da Silva)

10:55 AM IP-Rapid Microbiome and Multi-Omics Data Analysis: A Case Study on 

Inflammatory Bowel Disease (Dana Walsh)

11:05 AM Metabolomics Association of North America- Microbiome Interest 

Group (MANA-MIG) Panel Discussion: Martha Carlin, BioCollective

Andrew Percy, Cambridge isotope laboratories; Mary Lipton, PNNL;

Laura-Isobel McCall, Oklahoma U; John Ryals, Consultant; Di wu, 

UNC; Tucker Hallmark, Avanti Polar Lipids; Maryam Goudarzi, Sciex;

Tom Metz, PNNL
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12:05 – 1:30 PM LUNCH

1:30 – 2:35 PM SESSION 3 MODERATOR: JASON KRALJ (NIST)

1:30 PM V-Standardizing Differential Abundance Approaches for Microbiome 

Data Analysis (Baris Ozdinc)

1:40 PM IP-Exploring a Novel Microbiome Sampling Technique: A Benchmark 

Experiment Comparing DNA Yield and Community Profiles Between 

PDMS and Swab Collection Methods (Pheobe Mankiewicz)

1:50 PM IP-Identifying driving factors in wastewater processing for monitoring 

via metagenomics (Ishi Keenum)

2:05 PM IP-Differential Abundance Analysis from Biased Metagenomic 

Measurements (Ben Callahan)

2:15 PM IP-Relative Abundance Estimation under Complex Measurement Error 

(Amy Willis)

2:25 – 3:05 PM DISCUSSION GROUPS SESSION 1 STEPHANIE SERVETAS (NIST)

2:25 PM Introduction/Overview IMMSA working groups

2:35 PM Break out into working groups

3:05 – 3:30 PM Coffee BREAK

3:30 – 4:15 PM KEYNOTE 1 MODERATOR: SCOTT TIGHE (UVM)

KEYNOTE Phil Hugenhotlz- SeqCode

Day 2: Wednesday, Aug 24, 2022 (all times MT)

8:15 – 8:30 AM WELCOME Breakfast & INTRODUCTORY REMARKS

Welcome, recap of day 1

Scott Jackson

8:30 – 10:25 AM SESSION 4; KEYNOTE 2 MODERATOR: ISHI KEENUM (NIST)

8:30 AM KEYNOTE Rita Colwell

9:15 AM V-Risk assessment at the gastrointestinal health and One Health concept 

(Sangeeta Khare)

9:25 AM IP-Measuring Antibiotic Resistance Propagation via Horizontal Gene 

Transfer in Complex Microbial Communities (Nadrat Chowdhury)

9:35 AM IP-Role of bacterial motility in evolutionary mechanisms for acquired 

antimicrobial resistance (Lisa Stabryla) 

9:45 AM IP-Summary of microbiome data analysis from statistical point of view 

(Yan Wang)
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9:55 AM IP-Developing metadata standards to improve omics sample 

interoperability and data analysis (Montana Smith)

10:05 AM IP-Metagenomic NGS (MGS) Performance Metrics with an Organismcentric Approach (Jason Kralj)

10:25 – 10:55 AM Group Photo & Morning BREAK

10:55 – 11:40 PM SESSION 5 MODERATOR: SANDRA DA SILVA (NIST)

10:55 AM IP-Standardizing approaches for stable isotope probing (SIP) 

metagenomics (Rex Malmstrom)

11:10 AM IP-Characterizing the Gut Microbiome of Dietary Patterns using Pooled 

Samples: Assessing a Novel Methodological Approach to a Complex 

Web of Confounders (Leigh Frame)

11:20 AM IP-Longitudinal Changes in Mental Health Symptoms May Be 

Associated with the Gut Microbiome in Inpatient Treatment-Seeking 

Adults with Alcohol Use Disorder (Katherine Maki)

11:30 AM IP-A Meta-Analysis Study On The Effects Of Breastfeeding Practices 

On The Gut Microbiome In The Mal-Ed Cohort Study (Aya Kutbi)

11:40 – 12:10 PM DISCUSSION GROUPS SESSION 2

12:10– 1:40 PM LUNCH

1:40 – 2:25 PM SESSION 6 MODERATOR: STEPHANIE SERVETAS (NIST)

1:40 PM V-Analyses of Mock Community Standards using a Highly Rapid 

Nanopore Sequencing Protocol (Cory Bernhards)

1:50 PM IP-Benchmarking DNA recovery rates from aerobiomes sampled with 

electret filters (Harrison J. Trethowan)

2:00 PM IP-ASVs/genomes indexing database for integrating datasets from 

different microbiome studies/Cultured-based quality evaluation of stool 

collection kits (Liping Zhao)

2:15 PM IP- Towards Genome Authentication: The ATCC Genome Portal

(John Bagnoli)

2:25 – 2:45 PM Coffee BREAK

2:45 – 3:30 PM KEYNOTE 3 MODERATOR: BHARATH PRITHIVIRAJ (RECKITT)

KEYNOTE Jack Gilbert
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Day 3: Thursday, Aug 25, 2022 (all times MT)

8:15 – 8:30 AM WELCOME Breakfast & INTRODUCTORY REMARKS

Welcome, recap of day 2 

Scott Jackson

8:30 – 10:20 AM SESSION 7 MODERATOR: ISHI KEENUM (NIST)

8:30 AM KEYNOTE Krista Wigginton

9:15 AM IP- Culture-free discovery and host-attribution of phages in diverse 

microbial ecosystems using high-throughput proximity ligation 

sequencing (Ivan Liachko)

9:25 AM V-Performance comparison of host DNA depletion methods to allow 

direct sequencing of blood samples for pathogen identification (Daniel 

Evans)

9:35 AM V-ViOS - an open access systems biology platform for next generation 

of diagnostic and therapeutic approaches (Momo Vuyisich)

9:45 AM V-Galileo ONE: Standardizing metagenomics research using a sampleto-result workflow with integrated controls (Meredith Carpenter)

9:55 – 10:25 AM Morning BREAK

10:25 – 12:00 PM DISCUSSION GROUPS SESSION 3

12:15 – 1:15 PM LUNCH

1:15 – 2:05 PM DISCUSSION GROUPS PRESENTATIONS 

MODERATOR: STEPHANIE SERVETAS (NIST)

DISCUSSION GROUP PRESENTATION TIMES AND TOPICS SUBJECT TO CHANGE

1:15 PM Reference material WG report

1:25 PM Bioinformatics WG report

1:35 PM Metabolomics WG report

1:45 PM Documentary standards WG report

1:55 PM Enumeration and viability WG report

2:05 – 3:35 PM Closing Remarks and End Reception